LOCUS       DBNGSV010000001         5334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1108263, whole genome shotgun sequence.
ACCESSION   DBNGSV010000001 DBNGSV010000000
VERSION     DBNGSV010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1108263"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC159U_00005"
     CDS             complement(<1..418)
                     /locus_tag="AC159U_00005"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3335472.1"
                     /translation="MSKYNKDLVCSKITNRHIGSRLIFLDEVDSTNKYALSIIDQKPC
                     VSGTIVVADSQYAGKGRLDRSWYSPKGVNLYLSVILIPDIKTEEVNILTFVSSVATVN
                     TLLEYGLSPEIKWPNDVQVNRKKISGVLTEINMEGNK"
     gene            556..981
                     /locus_tag="AC159U_00010"
     CDS             556..981
                     /locus_tag="AC159U_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3335473.1"
                     /translation="MSDTEKKDIIRSNETFYKALGTRDLKLMESVWANDDKSCCVHPG
                     WIMFYGWDSIKQSWENVFDPEDQVDIKLTNINLEVKGNVAWLTCIQHLTYIKRDPMMA
                     NVSQSTNIFEKKGSTWLMVLHHASPVPVTEYEVPNTTLQ"
     gene            complement(1010..4264)
                     /locus_tag="AC159U_00015"
     CDS             complement(1010..4264)
                     /locus_tag="AC159U_00015"
                     /inference="COORDINATES: protein motif:HMM:NF053442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3335474.1"
                     /translation="MIKYIFILIAAILLNVQLAASDEQYTEADAKMLRFPDVSQDKIV
                     FVYAGDIWSVGKSGGVAARLSSPKGQEFFPKFSPDGTQIAFSGNYDGNIDVYLMPSEG
                     GEVKRLTHHPDDDYVVEWYPDSKDILFKSQMLSPIRRFNKLFKQSVQGGLAQKLPLPF
                     GEWASFNSDGSKMTFQIVSRESRTWKRYRGGMASDIWVYDFGEDKVSKLTNFEGKDSL
                     AMWHGNSIYFISDRGENKRNNIWKLNTSNNEITQVTHFKDFDIKWPSLGPDSIVFENG
                     AKIYLLDLKTQKSTHISISVPADLPDIRPRLKNLEKYIDSYELSPSGRQALFEARGEV
                     FLVGAKNEIIKNITNSSGSAEKYPTWSVNEDKIAYLSDSTGEYQLHILNLSDLTTDVI
                     ELEPGYYYNPVWSPDAKSVLLINNTGSLFIADIKTKEVKVIDTDNWEKIRYYTWSHDS
                     QFIAYSKKLDNYQSSIFIYDIKEGKSAQITSDYYNDYSPVFSSNGKYLAFFSDRHFNP
                     LYGDMDETWIYPGSTQLFIITLQKDLKSPFLPKGLNTQTDHENGDEKEKRNRSRKNTN
                     TLKIDFENIENRVIKVPVSHSNYKRLEFKDDNLFYMKAPAARSHHSKNKGNLNLYSLK
                     DKKEKTLIKGINNYSLSYDGKSYLYKANNKYGVTTSDKEQKAGAGKLATNKISSFVDL
                     RKEWNQIFHEAWRIERDFFYDPNMHGVDWNAVRDHYSGLLKHVVSRDDLNYLIGEMVS
                     ELNSSHTYVKGGDLDNAHKISVGLLGCDFEYDSKNDAYFIKKIYKGGVWDEEVRSPLA
                     LPGINIAEGDYVLSVNGKKINTSVDPWAAFQNLAGEVAELEVSSPDKPNESKTVLIKM
                     LNRKADLRLRYLDWVESKRKKVDELTGGKVGYIYVPDTGTHGQNELVRQFIPQRTKHG
                     LIIDERFNGGGQVPDRFIELLNRPVYNYWARREQNSHQSPFVSHTGPKVMIINGWAGS
                     GGDAFPHYFKKTGLGKLVGKRTLGGLIGIGGFPRLVDHGYITTPNYAFLGTDGNWDIE
                     GYGVSPDYEVENMSSINPDNSDPQLQKAVDVILEMLNNRQEQKPVVPEYPDRSN"
     gene            4439..4528
                     /locus_tag="AC159U_00020"
     tRNA            4439..4528
                     /locus_tag="AC159U_00020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4473..4475,aa:Ser,seq:cga)
     gene            complement(4652..>5334)
                     /locus_tag="AC159U_00025"
     CDS             complement(4652..>5334)
                     /locus_tag="AC159U_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3335475.1"
                     /translation="HKGPGDCIVINAENITLDCKSFKISGSSASISGIRINSARSVKV
                     RNCTIEKFRTGIMDVNGVSNIIEFNTVRFNSGSGMSISSVDGAVMSNISNDNSTNGIS
                     VVGVSNYIYNNTVRSNNKNGIVIIGSGNNKAKVNIKNNKNRIFAKKIESNYIVKNRTT
                     SNGENGIFLLLKATGNLVYANVSEKNKQHGIMDHSPVNNKLLNTYTRNRCSDNLIGDS
                     SPKDLCEP"
BASE COUNT         1544 a         1114 c          873 g         1803 t
ORIGIN      
        1 gtttattccc ttccatatta atttctgtta ataccccaga gattttcttt ctgtttacct
       61 gcacatcatt tggccatttt atctcagggc tcaaaccgta ctccaaaaga gtgttaacag
      121 ttgctacaga tgaaacaaat gtaagaatat ttacctcttc ggtttttata tcaggaatta
      181 atattacaga taagtatagg tttactcctt ttggggaata ccacgagcgg tccaacctgc
      241 cctttcctgc gtattgactg tctgctacta caatggtacc cgaaacgcat ggcttctgat
      301 ctataatgct caatgcgtat ttattggtcg agtcaacctc atccaaaaat ataagcctgc
      361 ttccaatatg acgattggta atttttgaac aaactagatc tttgttatat ttactcatta
      421 cacttaatta taatacactt taaaaaacca agatttaagt taattttatt tacagttaat
      481 tatggtattt tattctgtgc cggattagat agaataagca tcctggattt agataaacta
      541 tgaaaatcaa acaaaatgtc tgatactgaa aagaaagata ttataaggtc taacgaaaca
      601 ttttataaag ctcttggaac aagggatttg aagcttatgg aaagtgtttg ggcaaatgat
      661 gataaatcat gctgtgtaca cccgggctgg ataatgtttt atggctggga ttcaataaaa
      721 cagagctggg aaaacgtatt tgaccctgag gaccaggtag atataaagct aactaacata
      781 aacctggaag ttaagggtaa cgttgcctgg cttacctgca tacaacacct aacatacatc
      841 aagcgagacc ctatgatggc aaacgtctcc caatcaacaa atatcttcga gaaaaaaggc
      901 tcaacatggc ttatggtgct tcatcatgcc tccccggttc ctgtcaccga atatgaagtg
      961 ccaaacacta cattacaata gtcttgtatt agatatctaa tttttattac taattggatc
     1021 tgtcgggata ttccggtact acaggttttt gttcttgtct gttatttagc atctccaaaa
     1081 ttacatcaac cgctttttgg agctgaggat cagaattatc cggattaata ctgctcatat
     1141 tttccacttc ataatccgga gatacgccgt agccttcgat gtcccagttt ccatcggtac
     1201 ccaggaaagc atagtttggt gtagttatat agccgtggtc cactaacctt ggaaaacccc
     1261 caataccaat aagccctcca agagttcttt taccaacaag tttgccaagc cctgtttttt
     1321 taaaataatg aggaaatgca tctccacctg aacctgccca tccgttaatt atcataacct
     1381 tgggtcctgt gtgagaaaca aaagggcttt gatgagaatt ttgctcgcgc cttgcccagt
     1441 agttataaac aggcctgtta agtagctcaa taaacctgtc aggcacctgc cctcctccat
     1501 tgaacctctc gtcaattata agcccatgtt ttgttctttg aggtataaac tgtcttacaa
     1561 gctcattttg accgtgtgtt ccggtatcgg gaacgtatat ataacctact tttccacccg
     1621 ttagctcgtc cacttttttt ctcttagatt ctacccagtc aagataccta agccttaaat
     1681 ccgcttttct attaagcatt tttatgagta cagtttttga ttcatttggc ttatcgggtg
     1741 aactcacctc taattctgct acttctccag caagattttg aaacgcggcc catggatcaa
     1801 cagatgtatt tattttttta ccatttacag ataaaacata atcaccctca gctatattta
     1861 ttccaggtag tgccagagga gagcgaacct cttcatccca tactcctcct ttataaatct
     1921 tttttataaa gtaagcatca tttttagagt catactcaaa atcgcatccc aaaagcccga
     1981 ccgatatttt atgtgcatta tctaagtcgc cgccttttac ataagtatga gatgaattga
     2041 gctcagagac catttctcca atcagataat ttaaatcatc cctgcttact acgtgcttga
     2101 gcaaccctga gtaatgatcc cttactgcgt tccagtccac cccatgcata ttaggatcat
     2161 aaaagaaatc tctttcgatc ctccatgctt cgtgaaatat ctgattccat tcctttctta
     2221 aatcaacaaa tgagctgatc ttgttagttg caagcttgcc ggctccggct ttttgttctt
     2281 tatcactagt tgttactcca tatttattat tagctttata taaataggat ttaccatcat
     2341 atgagaggct ataattgtta atacccttta ttaatgtctt ttccttcttg tcttttaatg
     2401 aatacaaatt caggtttcct ttgtttttgg aatgatggct tcttgcagcc ggagctttca
     2461 tatagaaaag gttatcatct ttaaattcaa gcctcttgta attactatgt gatacaggaa
     2521 cttttatgac cctgttttct atattttcaa aatctatttt tagagtatta gtatttttcc
     2581 tgcttctatt tcttttttcc ttctcatctc cattttcatg atcggtctga gtgttgagac
     2641 cttttggtaa aaatggtgat tttaaatctt tttgtaatgt aataataaaa agctgagtag
     2701 agcctggata tatccaggtt tcatccatat ctccatatag agggttaaaa tgcctgtctg
     2761 aaaaaaatgc cagatacttt ccgttgctgc taaacaccgg agagtagtcg ttgtagtaat
     2821 cacttgtaat ctgagcagat ttcccctctt ttatgtcata tataaaaatt gatgattgat
     2881 agttatcaag tttttttgaa taagcaataa actgactgtc atgtgaccat gtgtagtatc
     2941 ttatcttttc ccagttatca gtatctatta cctttacttc tttggtcttt atatcggcta
     3001 taaaaaggct gcctgtatta tttataagca aaacactttt tgcatcgggt gaccagacag
     3061 ggttgtaata ataacctggc tccaactcta taacatctgt agttaaatct gataaattta
     3121 aaatatgtaa ttgatactct cctgtactgt cagagagata agcaatctta tcttcattta
     3181 ccgaccatgt gggatatttt tcggcactcc ctgaactgtt cgtaatattc tttattatct
     3241 catttttagc acccacaaga aacacctcac cccttgcttc aaacagcgcc tgcctgccgg
     3301 aaggagaaag ttcgtatgag tcaatatatt tttcgagatt tttaagcctt ggtctaatat
     3361 cgggaagatc agcaggaacc gagatagaga tatgggttga tttttgagtc ttaagatcga
     3421 gcaggtaaat tttagcgccg ttctcaaaca ctattgaatc aggccctagg cttggccact
     3481 tgatatcaaa atctttaaaa tgagttacct gtgttatttc attatttgat gtgtttagtt
     3541 tccaaatgtt gttacgtttg ttctctcccc tgtcagagat aaagtaaata gaattaccgt
     3601 gccacatagc aagagaatct ttaccttcaa aatttgtaag ctttgatact ttgtcctcac
     3661 caaaatcata gacccatata tcacttgcca tgccacccct gtatctcttc cacgttctgg
     3721 actcgcgcga tactatctga aaggtcatct tgctcccatc agagttaaaa cttgcccatt
     3781 caccaaaagg gagaggaagt ttttgcgcaa gcccaccttg tacagattgt ttgaaaagtt
     3841 tattaaatct tcttatggga cttaacatct gggatttaaa aagaatatct ttcgaatccg
     3901 gataccattc cactacataa tcatcatctg gatgatgggt gagtcgttta acctctcctc
     3961 cctccgaggg cattaaatat acatcaatat tcccgtcata attaccgcta aaagctatct
     4021 gtgttccgtc gggagaaaac ttggggaaaa actcctgtcc ttttggtgag ctcagcctcg
     4081 cagccactcc gccgctttta cctacgctcc agatgtctcc tgcatataca aaaacgattt
     4141 tgtcctggct cacatcggga aaccttagca ttttagcatc ggcctcagta tattgctcat
     4201 ccgatgctgc caactgaaca tttagtaaaa tagccgctat tagaataaat atatatttaa
     4261 tcacttgttg tttacccttt atataaaagc tgtgatactg ctaaacctat taaattaacc
     4321 aataaatcat aaaataacta tatttttatc agattagaaa taaatccttg aatatagttt
     4381 cagaagttga cttaaattat ctaaatggaa aaatctcagg aggctaatat tatagcacgg
     4441 agggatggcc gagtggacga aggcgctggt ctcgaaaacc agtgtgcgag caatcgcatc
     4501 gtgggttcga atcccactcc ctccgccagt aaactttata agacccgttt tatttgacct
     4561 cttaactact aaagaaatat accctaatcc ttacggagtg aaagtaaagt tagcaatctt
     4621 gggtcatgaa tatcggatat aaattctcaa atcagggctc acacaaatcc ttgggacttg
     4681 aatcaccaat caaattatca gaacatctgt ttctggtata tgtatttagt aatttattat
     4741 ttactggcga atggtccatt ataccgtgct gtttgttttt ctccgaaaca ttggcataaa
     4801 caagattacc cgtagccttg agtagtagaa aaatcccatt ttcaccgtta gatgttgtac
     4861 ggttttttac tatgtaattc gattcaatct ttttagcaaa tattctattc ttgttatttt
     4921 taatattcac tttagcctta ttgttaccac ttccaattat tacaataccg tttttgttgt
     4981 tcgatctaac agtgttgtta taaatatagt tgctcacacc cactactgaa attccgtttg
     5041 tagagttatc gttagaaata ttactcataa ctgcaccatc cacactactt atgctcattc
     5101 ctgaacccga attaaacctc actgtgttaa attctattat attgcttacc ccgttaacat
     5161 ccattatacc tgttctaaac ttctctatag tacagttcct tactttcaca cttcttgcag
     5221 agtttattct aataccggat atactggccg agcttccgct tatcttaaag ctcttacagt
     5281 caagagtaat attttcagcg tttattacaa tacagtcacc cggcccttta tgaa
//
LOCUS       DBNGSV010000002        32001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_138592, whole genome shotgun sequence.
ACCESSION   DBNGSV010000002 DBNGSV010000000
VERSION     DBNGSV010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 32001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32001
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_138592"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC159U_00030"
     CDS             complement(<1..341)
                     /locus_tag="AC159U_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3335476.1"
                     /translation="MLSFIGTIIVIGIIAVFIITIVVSQFLLICHPNEVIVLSGRSRR
                     LKDGSIVGYRIIRGGRALRIPMVEKAARMSLETIPLDLKVDNAYSKGGIPLKVEAIAN
                     VKVDSSEPTFG"
     gene            complement(357..953)
                     /locus_tag="AC159U_00035"
     CDS             complement(357..953)
                     /locus_tag="AC159U_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335477.1"
                     /translation="MEKVYIACFILGFIFLLVSIFTGMDTDADIEINTDVDVDIDADL
                     DSDIGSNAHGEGAVEAVKFLSLRNIIFFVAFFGFTGTVLTYLKTEQYLTVASSVLMGL
                     FTAFVGHKALSYLKRTEVGEGKSLSQLVGHNAKVLVSVTKNSQGKISVTTDEQLIQIL
                     AMVADESTRDEFKSGDEVVIVKIEGGTAYVGEKEFINS"
     gene            complement(966..1673)
                     /locus_tag="AC159U_00040"
     CDS             complement(966..1673)
                     /locus_tag="AC159U_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR3335478.1"
                     /translation="MKLLARISKLITANINSLLDEAEDPEVMVKQLIRDIEESIVELR
                     RETVKAVANEKQLEKQLSSTVDLIIDLESKARLALEKDEEALAREVLKKKLRTEKTRD
                     NLTKEHKSSVNLSAQLKSDLVRLEDRAQEVRRKKEELIRRKRSAEAALRTQKAAKKSS
                     EIVSDVKSFIDDIERSITDVDSIENEIIKIESQAEAEKEVLELEIKKELDLQSLAEEN
                     SVNEELDRLKKDLNKTN"
     gene            complement(1801..2769)
                     /locus_tag="AC159U_00045"
     CDS             complement(1801..2769)
                     /locus_tag="AC159U_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3335479.1"
                     /translation="MSVNIQGTDNNYKPGVGSSSEFLDIIRQAETYASVPRPILVRGE
                     RGTGKELLAHYIHAISNRSEYPYVIVNCAAFQEDLFIADMFGHEKGAFTGADGTKIGK
                     FELANKGTFFLDEVANMSIKVQEKLLRVIEYQSFNRLGGTKKIEVDVRVIAATNAPLE
                     EMMENGEFLPDLYDRLSFAVLVVPPLRKRRDDIPLLLKHFIDQLHQEMPDLEIKSFTK
                     AAVKVLKEYHWPGNIRELKNIVERLYISDIDGVICASELPIEITSLEPLDGSFYGRTK
                     AFKKTILLTTLKDCLGNQREAAAKLGMTYDQFRHYYKKFNLGELLV"
     gene            2948..3325
                     /locus_tag="AC159U_00050"
     CDS             2948..3325
                     /locus_tag="AC159U_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3335480.1"
                     /translation="MDNKEVDILEMFKLSNRYDVFEPGDIIFDKGSDGGIMFIILEGE
                     IEIQVDGFAVGKLFEGQILGEMALVEDQPRSATAIAITHTKIVPINRETFLLIVKEEP
                     LFALFVMKDLSHKIRVLNQRLTN"
     gene            3488..3664
                     /locus_tag="AC159U_00055"
     CDS             3488..3664
                     /locus_tag="AC159U_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335481.1"
                     /translation="MSCNTVVHEVEGKPDHLMQFLDDYDTDTEHEAISKQHDLLIEIL
                     ETFRADKSVRNTEY"
     gene            3846..4058
                     /locus_tag="AC159U_00060"
     CDS             3846..4058
                     /locus_tag="AC159U_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR3335482.1"
                     /translation="MSERVIGTVKWFNASKGFGFIERENENDVFVHYSEISSDGFRSL
                     EEGQRVEFTVADNGPKGPQAQNVVAV"
     gene            complement(4164..6746)
                     /locus_tag="AC159U_00065"
     CDS             complement(4164..6746)
                     /locus_tag="AC159U_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3335483.1"
                     /translation="MKTNLFFSFIALMLLSVIFASNPLSASKVLTGEVEAYIAEDFDS
                     FIQEDKSIIIPGKGVYKLKNSKNEPDNRYSDLITGDKVKIKTGGSLSGKSSIVLTKNP
                     NIENLPPQAQSAFANIIGIIPNSQADGTENTEQIADGPSAQAAGDPSEMRTVITFLVD
                     WGTGYTVANPDPMMNNMCDASDSTDVVWRDSSYNQLGLNCDLNNDTNNDVFGPYSVPV
                     PSNPCAFSNLVSMGNAVMNSARDAGVNVDAYQHKAFILPPELSCGWDGYAHIGCSSSS
                     CHSFYDGSSLNWPVLPTHELLHNYGMHHASTDTDNDCTVNSEYGDRSCDLGNPYGNIH
                     VNAFHKNQYSWIPTAAKPIAQTSGTYQIDPLNRDPGTLTDPILMLRIPLQDINGQYFY
                     VSNRQPEGIDTNLRTEYCNTVSVHRKCSSNSRSYLVANLAVGDTFTDAARNITITHDS
                     RTADHANVIVSLDEGSDITPPSVNITLPTDGETVSGVFNAIANASDNVGIDRVEFYMD
                     AEATPYCTVTGDQPSYSCNIDSTLYSFGAHTVTAWAYDANDNSASDSANINVDNLPPD
                     ITPPDVSITSPLNGDEVSGDVTVQVNATDNETVDFVTVLVDAVEVCPSLTQTPYQCTF
                     DSTQFTNGQRAITARAYDLEPNMGEDSIAVTVNNLAPLAITSSGLTNKTSSSADVNVS
                     TNIPTNAQVNYGLSKNSLNNSATDGVSDTNHTINLTGLSANTWYHYQIVVDSADGQSE
                     TTTTKKFRTSRSSGGGDPTPTPTPPPSGSLSIIDSGVQNKTSSSAEVVSTISGPSTVV
                     VNYGTSNNNLSQSATDGVTDTNHTVLLSGLQSSTRYYFEIVATSEDGTEQTTSGVSQF
                     RTRR"
     gene            complement(7075..7458)
                     /locus_tag="AC159U_00070"
     CDS             complement(7075..7458)
                     /locus_tag="AC159U_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR3335484.1"
                     /translation="MNWYIEVLKKYAVFGGRARRKEYWIFSLIHIIVCFVLTFIDSQL
                     GLLKDEGPGVIGGLYILATLIPNIAVSIRRVHDVGKSGWWLLILFVPFIGGILLLYYL
                     VKDSDPGQNQYGPNPKGESAGVNIT"
     gene            complement(7542..8780)
                     /locus_tag="AC159U_00075"
     CDS             complement(7542..8780)
                     /locus_tag="AC159U_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3335485.1"
                     /translation="MPESALIYIAVLIIGIVIGVAVSYLLEIMQGKNAKELAEEIFSE
                     NESKRQENIDQVISNMKESFGSMSLEALSKSTEQFLALAKSTLEPEREFTKQELETKK
                     GLIDQQLGKMTGELEKVSTLMKELEKDRVEKFGQLSNQLRITNQQTSELMKTTGSIRE
                     ALASTKTRGQWGERMAEDVLRLAGFVENINYVKQKTLEGSTQRPDFTFMLPKDLTINM
                     DVKFPLNNFVNYLEAQSERDKEGYRKTFLNDVKLRLKEVTTKDYISPESNTVDYVILF
                     IPNEQIYSFIQEEDSAIFDTAIQNKVLLCSPVNFFAVLAIIRQAIDNFALEQTSKEML
                     VQFGAFKKQWDAFVAKFDTLGQRIDAVQKEYEVLTTTRKRAVERPLNKIEGIRKQKGL
                     PSSDDEDDQIPKFEVVDSDS"
     gene            complement(8817..9347)
                     /locus_tag="AC159U_00080"
     CDS             complement(8817..9347)
                     /locus_tag="AC159U_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR3335486.1"
                     /translation="MDKLVERMIRAAKLDVDLYEEVEADKSTMTQAVVVVIISSLAAG
                     LGRFGELDLSGIVIFIILSLAGWFIWAYITYIIGTKLLPQPQTKADHGELLRTIGFSS
                     SPGVLRAFYFIPVVGGIITSIAVVWMLVTMIIAVRQALDYDSTLRAVGVCIIGWIIQA
                     FFILFLSSIFDIPIMK"
     gene            complement(9350..10507)
                     /locus_tag="AC159U_00085"
     CDS             complement(9350..10507)
                     /locus_tag="AC159U_00085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3335487.1"
                     /translation="MSPDSSYKLQFQGLYYDGRSARRHDVEVTLTRDSLRIDADTFSN
                     TITWLYPRITQVKDIYSENEVRLQYGQELPEILIIRDKSFIGSLRSISPQFGRNFSRH
                     GSDKRIKTVAYVFIALFISAVLLHKWIVPPLMNIMVEKFPVTWEEKIGKSFISQISKG
                     FGVCEDERLNTQIDKIKDRLGATLDSSKYKYNITIVNNSLVNAFALPGGEIILFRGLI
                     EKSNRPEEVAGVVAHEIQHIEQRHFTKILIKELSIGIFLGLMIGDSQVVGTALNYANM
                     LRSLAYKRSEESSSDAEGMKMLIKAGIDPKGMVDFFEIMKKEYDGTTEVSEYLSTHPN
                     TENRIEKLNTMIAQKQIESEKLLPNVDWEKTKMLCQETKEQQDVKMSEEDK"
     gene            complement(10517..12112)
                     /locus_tag="AC159U_00090"
     CDS             complement(10517..12112)
                     /locus_tag="AC159U_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HIR3335488.1"
                     /translation="MKCPNCNAIVPGSWKTCRCGYNLMSSQPVATPLYKKNVESAKLT
                     EKRRKFIEKTSRPAPADSAATKRVSEDATQILDTSDLMAKQQAEMQKAKQSIDTRADS
                     DATQIIDSESLVAKQKAEMLKASEAIRKRYKGGDSTQILDVTETPIQMTNPNLQTPAA
                     QVRSSINTEGIAPGDAGSTGNVSSGVGSYRRINFFGNGTTLLGIYIVNILLMIPTLLI
                     YFFWGKIRAARYLYSQADLDGDRFEFHGTGLELFLGFIKAILAIGVVIGSAFAIMYLV
                     PGVIAKILGGIFIYIVIVLLIPLAILFTVKYRYSRTSWRGVRFSFRGNTKEFLKLYVK
                     GFFLTSLTAGFYYYSFHYRTREYLINNIYFGDTKFEFDGNINDLYSRYTKLWLVAILI
                     SGAHQLLTVFMPTIGTDILDIGYFVLFIIWGIFFLKFLAQRHNHYWNHTKFVSARFNL
                     NIEEKDYVKLQIVNYILLIFTLGIAYPYVQIRNIKFLLDRLSLQGDLDLIAIQQDART
                     PTALGEEVASFLDIDLVGMDLGI"
     gene            12279..13499
                     /locus_tag="AC159U_00095"
     CDS             12279..13499
                     /locus_tag="AC159U_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3335489.1"
                     /translation="MELINLISIIISVSAAFSYINYRFIKLPTTIGVLVIAIICSLGL
                     IALSSLGFIEFEKNAKLIISSIDFDKTLLHGMLSFLLFAGALHVNINDLAKQKWVIST
                     LASIGVISSTFLIGTLTWLILSFLNINLDFIYCIVFGALISPTDPIAVLGILKKADIP
                     KSLETKVVGESLFNDGMGVVIFLVLYEIAFSGHTFDPSNALVLFLTEAVGGAVFGFAA
                     GWVVYKLMHSVDNYHVEILLTLALVMGGYSLAMYLHLSGPIAIVVAGLLIGNRGRILA
                     MSDNTRENLDTFWELIDEILNVILFVLIGLEILILSFSKSYIYASLLIIPVILLCRFI
                     VVYIPVSILKYLRDFSPNAVKIMTWGALRGGISVALALSIPAGEQREIIVSITYIVVV
                     FSIVIQGLTISKLT"
     gene            complement(13508..14497)
                     /locus_tag="AC159U_00100"
     CDS             complement(13508..14497)
                     /locus_tag="AC159U_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335490.1"
                     /translation="MKNLKTFFKNLGADYKNLGIFAAVIVFIIFGCLLYYQNQRVKQY
                     RSDLAKLDQQIDEKKAEINEQVNILEGQQKNLEHKFSDLTMIISDQIYSDQIGKDFFK
                     FIEDPNGRKLYRDQQKIVVNEIYEDLMVDLFLNESEENELIGLLVDKQMVNLEIIVSL
                     LKGDMNEAQIVENEKKFNEMIARADNNLRSFFEDDEFELFEEYNLTLQYRSWILEFKT
                     YLADRQIFLDLDQEEDLLFLILNESEDFNFTERLQANEFNSGKASEVDLARINRYMLE
                     KEELDQIITDKSQTFLDERQLKALEDYLKLKRTMDEMGFEITKDIAPTPTPVK"
     gene            complement(14503..14709)
                     /locus_tag="AC159U_00105"
     CDS             complement(14503..14709)
                     /locus_tag="AC159U_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR3335491.1"
                     /translation="MKKLYRSEKNKMVAGVCGGLAEMFDIDPTLVRLGLVFLCLVTAL
                     VPITIAYIFGWIIIPTESELNNNS"
     gene            complement(14717..15163)
                     /locus_tag="AC159U_00110"
     CDS             complement(14717..15163)
                     /locus_tag="AC159U_00110"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR3335492.1"
                     /translation="MKYFAYGSNMNIKRMEQRGVTVFKRAKACLFGYTLRFNKTTYTF
                     GGAGHANIVENENELVQGILYEIDNNGIFNLDKYESYPVEYDRRLMSVILKNGVEKEA
                     YVYIAQPTRTSDDLIPTKEYLSHLLCAEGLLSADYMSRLNTQKTID"
     gene            complement(15461..16474)
                     /locus_tag="AC159U_00115"
     CDS             complement(15461..16474)
                     /locus_tag="AC159U_00115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3335493.1"
                     /translation="MNGIGLILDAHNISDLAILAKQAEDHNFHSVWATELYRTSFQQL
                     SVAAHATKKIKLGTAVALAFTRSPFITAITSMDIDEISGGRLILGLGSGAKRTNEMWH
                     GVEHGKPVTKIKECIHIIRNVINSSHKKEDFTFEGEYFDINTKGYYRAYNPVRENVPI
                     YLAGVGAKMTEASGQVADGYLGHVVCSQEYLKKVVIQSINNGLSNSRKNRKDFTIASI
                     ITCAISENKKEALEAAKATIAFYATVKTYEAPFKLHGFEGECKKIREAYFKRDIGTMI
                     STVTDSMVNTFAVVGTADECREKINDYKQLIDLPILSAPHYFIDSSKVQKYQQAILNT
                     FGG"
     gene            complement(16846..17349)
                     /gene="rplQ"
                     /locus_tag="AC159U_00120"
                     /pseudo
     CDS             complement(16846..17349)
                     /gene="rplQ"
                     /locus_tag="AC159U_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(17390..18421)
                     /locus_tag="AC159U_00125"
     CDS             complement(17390..18421)
                     /locus_tag="AC159U_00125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3335494.1"
                     /translation="MKDLQLNWQDLIKPKRIVIDEKSSTEYYGRFICEPLEKGYGITL
                     GNALRRVLLSSIKGPAITSIKVEGVLHEFSTIPGVKEDVTQIILNLKGLQLKVHTFEK
                     QTITISAQGECDVTAGDIITTHQVDVLNPDHHIATLSEGSKLEMEMTVEVGYGYEPVE
                     SRGDIQTSIGLIPLDAIYSPVRKVNYSITNARVGRRTDYERLNLEIWTNGTVIPEDAV
                     AYAAKIIKEQLTVFINFDEEEVDAIIEVEDDKEISGTEEILFTSIEDMDLSARSLNCL
                     RKAEIAYVGELIQKSEDDLLNLENFGKRSLLEIRERIEEMELSLGSVIDVEAFESEKQ
                     KMLETEESA"
     gene            complement(18471..19103)
                     /gene="rpsD"
                     /locus_tag="AC159U_00130"
     CDS             complement(18471..19103)
                     /gene="rpsD"
                     /locus_tag="AC159U_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3335495.1"
                     /translation="MSRYIGSVCRFCRREGMKLFLKGDRCFSEKCAVDKRPEQVPGDH
                     GLRRPKFSEYGIRLREKQKVKRMYGLTEKQFSRYFETASRKKGVTGSLLISLLERRLD
                     NVVYKLGFASSRKEARIMVRHGHFMVNGRKVDIPSYQLQLNDTIQVSEKSNKVGRILQ
                     SLEGLARRGFPEWLEIDQEKLMGTVKRLPEREDVTIPIEEQLIVEFYSRV"
     gene            complement(19148..19531)
                     /gene="rpsK"
                     /locus_tag="AC159U_00135"
     CDS             complement(19148..19531)
                     /gene="rpsK"
                     /locus_tag="AC159U_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3335496.1"
                     /translation="MAKKKTTKKAKKFLEEGIVHIQATFNNTVVSISDKAGNVVAWSS
                     GGAKGFKGTRKGTPFAAQIASDDAAKKAASMGLKSVSIHIKGPGPGREPAMRSIQGAG
                     IKITMIKDVTPIPHNGCRPPGKRRV"
     gene            complement(19550..19930)
                     /gene="rpsM"
                     /locus_tag="AC159U_00140"
     CDS             complement(19550..19930)
                     /gene="rpsM"
                     /locus_tag="AC159U_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3335497.1"
                     /translation="MPRIAGVDIPEKKRIEISLQYIYGVGKDISKKILTEAEIDFSTK
                     SSDLSEQDIARIRDIIDSKFIVEGDLRREVQTNIKRLVEIGCYRGVRHRSNLPVRGQQ
                     TQSNARTRKGRKGTAIAKKKKVKG"
     gene            complement(19945..20058)
                     /gene="rpmJ"
                     /locus_tag="AC159U_00145"
     CDS             complement(19945..20058)
                     /gene="rpmJ"
                     /locus_tag="AC159U_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3335498.1"
                     /translation="MKIRASVKKICDKCKVIRRKGAIRVICVNKRHKQKQG"
     gene            complement(20077..20298)
                     /gene="infA"
                     /locus_tag="AC159U_00150"
     CDS             complement(20077..20298)
                     /gene="infA"
                     /locus_tag="AC159U_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3335499.1"
                     /translation="MAKEERLEFEGTVVEALPNAMFRVEIEGGHIILAYVSGKMRTRF
                     IRILPGDKVKLEISPYDLTKGRITYRLKK"
     gene            complement(20302..21072)
                     /gene="map"
                     /locus_tag="AC159U_00155"
     CDS             complement(20302..21072)
                     /gene="map"
                     /locus_tag="AC159U_00155"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3335500.1"
                     /translation="MIVLKNKEEIDKIRSSCSIVAEVLSALSEKAQPGISTYDLDRIA
                     EDMAIKSGAKPAFKGYRDYPGSVCFAINQEVVHGIPSKKKIVEEGDIVGMDFGIYKDG
                     FYGDSAITAAIGVVSDEAVNLLRITKEALYKGIEQAKPKNNLFEVSKAIQKHVESNGY
                     SVVRTFVGHGIGASLHEDPQVPNFVPKNSGWSKGGKLKAGMVIAIEPMVNVGSPDVKI
                     LKDGWTAVTIDGCLSAHFEHTVAITENGPEILTESGVH"
     gene            complement(21079..21639)
                     /locus_tag="AC159U_00160"
     CDS             complement(21079..21639)
                     /locus_tag="AC159U_00160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3335501.1"
                     /translation="MNLIIFGPPGAGKGTQAVNIVEKYGVAHISTGDILRGAVKNGTE
                     LGQKAKEYMDKGELVPDELIIGIIKDRIKEQDCQGGFLLDGFPRTIAQANALETMLSN
                     EGLEIDSVVSVEVQDSEIITRILKRAEEEGRADDTEDVVKNRLQVYRNQTEPLKDYYK
                     EKGKLSEVDGIGSVDEVFSRIAGTIG"
     gene            complement(21674..22984)
                     /gene="secY"
                     /locus_tag="AC159U_00165"
     CDS             complement(21674..22984)
                     /gene="secY"
                     /locus_tag="AC159U_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3335502.1"
                     /translation="MNSGISSLPKIPELNKRLLFVAFMLVVYRLGVFVPIPGVDSEQI
                     LDIFNQQASGTIFDMLNLFSGGALERASIFALGVMPYITSSIIMSLLVKAFPTLEAMQ
                     KEGEAGRRKINQYSRYGTILICLVQGGMLAATLNSGQLGNVVTNPGFSFVLTTIITLT
                     AGSMFVMWIGEQISERGIGNGISLIIAASIITTVPGAFWDLVRLIRTGEMTIFGTVIL
                     FVFLGCIVAAIVFVERSFRKIPVQYPRRVVGRKVYGGQSSYLPLKVNTAGVIPPIFAS
                     SLLFFPATILQFAEIPTLQDISSLILDNGLFYNLIYAALIIFFAYFYTAIIYNPDDLA
                     ENLRKNGGFVPGMRPGKKTSEYIDKILSRLTFIGAIYIAVVCIMPSVLRLDPFNVPFF
                     FGGTSLLIVVGVTLDTVQQIESFLINHSYEGLVKKKGMKGPRRY"
     gene            complement(22981..23409)
                     /gene="rplO"
                     /locus_tag="AC159U_00170"
     CDS             complement(22981..23409)
                     /gene="rplO"
                     /locus_tag="AC159U_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3335503.1"
                     /translation="MLNELKPTDGAKKKRKRIGRGPGSSGKTSGKGHKGQRSRSGRSV
                     PLWFEGGQTPLKMRSPKRGFTNINKVEFDVVNVGRLDIFESGEVTPEILREKGLVSGN
                     SPIKILGNGDLSKSLNIKANGFSASAKEKIEKQGGTIEII"
     gene            complement(23470..23988)
                     /gene="rpsE"
                     /locus_tag="AC159U_00175"
     CDS             complement(23470..23988)
                     /gene="rpsE"
                     /locus_tag="AC159U_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3335504.1"
                     /translation="MSNEKINPNGLDLKEKIVHIRRVAKVTKGGKRFHFTALVVIGNE
                     DGIIGSAIGKSNEVPNAIKKAIDKAKKKLIRVPRKGHTIPHEIIGEHSSSRIMLKPAG
                     PGTGVIAGGAVRAVIELSGIGDILSKVMGSTNPINVVNATIQGLSSLSMADEIAAIRD
                     KQLSDLDIPAAR"
     gene            complement(24022..24396)
                     /gene="rplR"
                     /locus_tag="AC159U_00180"
     CDS             complement(24022..24396)
                     /gene="rplR"
                     /locus_tag="AC159U_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3335505.1"
                     /translation="MIKVKSRKSMKNLRHRRVRKKVAGNSQTPRLSVFKSLSHIYAQV
                     IDDTTQNTLVSCSTLTPKISELIKKDKLGKAEASKLVGQELGDRCKEKGINTIKFDRG
                     GFPFTGRVKSLAEAVREKGIKF"
     gene            complement(24400..24954)
                     /gene="rplF"
                     /locus_tag="AC159U_00185"
     CDS             complement(24400..24954)
                     /gene="rplF"
                     /locus_tag="AC159U_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3335506.1"
                     /translation="MSRIGNNPITVADNVKVEFKQGIVKVTGPKGNLSRTYSDLIKIK
                     LEDGKLIFERSSDDNKAKALHGLTRSLIANMVTGVSEGFTKTLEIVGVGYKAEEAGKQ
                     GIKFSLGYSNPIDFVLPEGISSVIEERGTRVILSGIDKEAVGQTAAKIRQLRVPDAYK
                     GKGVRYQGEQPRLKPGKSGAKTGA"
     gene            complement(24967..25362)
                     /gene="rpsH"
                     /locus_tag="AC159U_00190"
     CDS             complement(24967..25362)
                     /gene="rpsH"
                     /locus_tag="AC159U_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3335507.1"
                     /translation="MTDPISDMLSRIRNALIAGHPSVRIPDSKIKSEILRVLKDEGYI
                     REVKEVQENKFKYLDVELKYHGSKKESVVKKLTRVSKPSRRVYVGSTQIPRVSGGLGT
                     SIISTSKGIMAGRAAREQKLGGEFICTVM"
     gene            complement(25396..25581)
                     /locus_tag="AC159U_00195"
     CDS             complement(25396..25581)
                     /locus_tag="AC159U_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3335508.1"
                     /translation="MTRKALIEKSNKEPKYKVRVKNRCPLCGRPRSFMRKFGMCRLCF
                     RKLASFGKLPGVRKASW"
     gene            complement(25595..26149)
                     /gene="rplE"
                     /locus_tag="AC159U_00200"
     CDS             complement(25595..26149)
                     /gene="rplE"
                     /locus_tag="AC159U_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3335509.1"
                     /translation="MSARLKTHYEQQVIPQLKEKFNYNNIMQVPKLHKIVLNMGLGKL
                     SEAGKDYKLIEDAASELSLIVGQKAVITRAKNSIAGFKLRENSAVGCMVTLRGTRMYE
                     FFDRLVSIALPRVRDFRGTSAKAFDGNGNYTLGLRDQTIFPEIDYNKVEKTKGMNISI
                     ITTAINDEQAKELLIGMGMPLVKQ"
     gene            complement(26149..26493)
                     /gene="rplX"
                     /locus_tag="AC159U_00205"
     CDS             complement(26149..26493)
                     /gene="rplX"
                     /locus_tag="AC159U_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3335510.1"
                     /translation="MKTNAIKKNSTHIKTGDTVYVIAGKEKGKTGKVLKIDRVKNKAV
                     VEKLNIAKKHTKPTQKSPTGGINEVEVGIPVSNMLLYCKKCAKPSRAGNKVLENGEKI
                     RYCKRCEEEFKD"
     gene            complement(26495..26863)
                     /gene="rplN"
                     /locus_tag="AC159U_00210"
     CDS             complement(26495..26863)
                     /gene="rplN"
                     /locus_tag="AC159U_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3335511.1"
                     /translation="MIQHHTVLDSADNSGAKNLFCIKVLGGSSRKYARIGDLIVVSVK
                     EAIPNSKVEKGSIQKAVVVRTRKEQRREDGSYVRFDDNAAVLITKDMEPIGTRVFGPI
                     ARELRNKGFMKIISLAPEVV"
     gene            complement(26860..27168)
                     /gene="rpsQ"
                     /locus_tag="AC159U_00215"
     CDS             complement(26860..27168)
                     /gene="rpsQ"
                     /locus_tag="AC159U_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3335512.1"
                     /translation="MARAKARTRIGVVKSNKMDKTVVVEIVSTVKHKVYNKPIKRSST
                     YKVHDKENKCSVGDKVQITESKPISKTKRWRVSKIIQQKVVPDLQIKDEAAVVAGEDQ
                     "
     gene            complement(27173..27376)
                     /gene="rpmC"
                     /locus_tag="AC159U_00220"
     CDS             complement(27173..27376)
                     /gene="rpmC"
                     /locus_tag="AC159U_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3335513.1"
                     /translation="MKISEIRNLSSDQIAKKLEESKNELFNIRVQVSTQQNTKVSEVG
                     RVRKLIARLNTVQRENELKGAKE"
     gene            complement(27373..27798)
                     /gene="rplP"
                     /locus_tag="AC159U_00225"
     CDS             complement(27373..27798)
                     /gene="rplP"
                     /locus_tag="AC159U_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3335514.1"
                     /translation="MLQPSRTKYRKQFKGRLRGKATKGDYLAFGDFGLQATENGMITS
                     RQIESARIAITRHVRRGAKLWIRIFPHKPITKRPAETRMGKGKGDVDGYVAPIKRGTI
                     LYEIGGLNDELAKEAFRLASHKLPLATRMVRREEGISIR"
     gene            complement(27811..28479)
                     /gene="rpsC"
                     /locus_tag="AC159U_00230"
     CDS             complement(27811..28479)
                     /gene="rpsC"
                     /locus_tag="AC159U_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR3335515.1"
                     /translation="MGQKVCPIGLRIGITKSWDSKWFAEKQKYTRLLHEDIKIRKVIK
                     DQFYQAGISKIELERAASERVRVVIFCSRPGMIIGRKGQEIETLRKRLGSMTGKDIYL
                     DIREVKRPELDAQLVAENVALQLQRRVAYRRAMKRSMSSSQRLGAIGIKIMVSGRLGG
                     AEIARKEWYREGRVPLTTLRADIDYGLAEAKTTYGIIGIKVWIFKGEVLEKKTSRQQP
                     QVAS"
     gene            complement(28481..28819)
                     /gene="rplV"
                     /locus_tag="AC159U_00235"
     CDS             complement(28481..28819)
                     /gene="rplV"
                     /locus_tag="AC159U_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3335516.1"
                     /translation="MVSSATLKFARLSHKKTKIVLDEIRGRGVEESFATLSLINRKAA
                     KIISKLLKSAVANASEKGHSDTDSLYIKEVFVNKGPFIKRIRPRAMGRAFSIKKKMSH
                     IKIVLEQRGL"
     gene            complement(28844..29128)
                     /gene="rpsS"
                     /locus_tag="AC159U_00240"
     CDS             complement(28844..29128)
                     /gene="rpsS"
                     /locus_tag="AC159U_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR3335517.1"
                     /translation="MGRSSKKGPFVDPKLQKKVDKAKETNDQKVIRTWSRSSMITPDM
                     IGLTFAVYNGRKFIPVYVTEHMVAHKLGEFSPTRTFYSHAGGDRKGKGRK"
     gene            complement(29142..29984)
                     /gene="rplB"
                     /locus_tag="AC159U_00245"
     CDS             complement(29142..29984)
                     /gene="rplB"
                     /locus_tag="AC159U_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3335518.1"
                     /translation="MGIKKYNPTSAGRRFMTVSDYSDISSKKSHKPLTIKLKKTGGRN
                     SEGRISVWHRGGGNKKLYRIIDFKRDKIDIPAKVSTIEYDPYRTSRIALLVYKDGEKR
                     YITAPDGLKVGDEIVSGNDVEIKTGNSLPLRRIPDGTTVHNIELKPGNGAKLSRSAGT
                     SAQIMAKEQKYAQIKLSSGEIRLVPLDCRATIGRVGNTDHELIVLGKAGRKRYRGKRP
                     VVRGVAMNPVDHPHGGGEGRATKGNPHPVSPWGWITIGYKTRNNKRTDKYILKRRRIG
                     YGMD"
     gene            complement(29987..30274)
                     /gene="rplW"
                     /locus_tag="AC159U_00250"
     CDS             complement(29987..30274)
                     /gene="rplW"
                     /locus_tag="AC159U_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3335519.1"
                     /translation="MNHPTQVIKKPLITEKSSMVKEAGWYLFSVDKKSNKIEIKNAVE
                     KLFKVKVDRVRTSISPGKTVKKYGRAIGKTSSVKKAYVKLKEGNIEFFEGV"
     gene            complement(30271..30888)
                     /gene="rplD"
                     /locus_tag="AC159U_00255"
     CDS             complement(30271..30888)
                     /gene="rplD"
                     /locus_tag="AC159U_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3335520.1"
                     /translation="MADVFDINKKKVNTIDLATTVFEAPVNRDIIYRVINWQLAKKRS
                     GTACTKTRGEVSGGGSKPWKQKGMGRARSGSNRSPIWRKGGVTFGPKPKDWSFSLPKK
                     VRRKALLSALSHANSENGVIVLDDFKASEIKTKNVSAILDKFDINSTLLIESGNYEVL
                     KKSSKNLSNVKVLKPEGLNVYDILKFKNIVFTVDSLTKVQEVLAH"
     gene            complement(30902..31543)
                     /gene="rplC"
                     /locus_tag="AC159U_00260"
     CDS             complement(30902..31543)
                     /gene="rplC"
                     /locus_tag="AC159U_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3335521.1"
                     /translation="MIEGLIGKKKGMTELFLETGEVIPTTIIEAGPCYVVDKKTEKRD
                     GYDSVQLGFAEIKPQRSNKAMSGVFKKAGVPPLGLLKEFKVLNNDSDKYNAGDVVSAD
                     IFAEGDYLDVTSVSKGKGFSGAMKRHRFSGQRDSHGGMAHRRVGSIGQASFPAKVWKG
                     QKMPGHYGARKFTIQGLKVVKVDIENNLVLVKGSVPGPNGSHIFLKHTVKGRV"
     gene            complement(31550..31870)
                     /gene="rpsJ"
                     /locus_tag="AC159U_00265"
     CDS             complement(31550..31870)
                     /gene="rpsJ"
                     /locus_tag="AC159U_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3335522.1"
                     /translation="MSRVPKIRIKLKSFDHKLLDKSTLEIVDTVKRTGARIAGPVPLP
                     TRISRYCVLRSPHVDKNSREHFEIRTHKRLVDILEPMQQTVDALMKLDLASGVEVEIK
                     LTGN"
     gene            complement(31886..>32001)
                     /locus_tag="AC159U_00270"
     CDS             complement(31886..>32001)
                     /locus_tag="AC159U_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3335523.1"
                     /translation="NMVVELITEVAMEEKLRFAIREGGRTVGAGVVTKILE"
BASE COUNT         8682 a         7082 c         5162 g        11075 t
ORIGIN      
        1 tttccaaacg taggctccga tgaatcgacc tttacgtttg caatagcctc taccttaaga
       61 ggtataccgc ctttcgaata cgcattgtca acttttaaat caagagggat agtctccagt
      121 gacattcgcg ccgctttttc aaccattggt atcctaagtg ccctgccccc tctgattatc
      181 ctgtaaccta ctatagatcc atcctttaat cttcttgacc gtccggacaa tactattact
      241 tcatttggat gacaaatcag taaaaactga cttaccacaa tagttattat gaatactgca
      301 ataataccga ttacaattat cgttcctata aaactaagca taatataaac ccccttttat
      361 gaatttataa actccttttc tcccacatag gcagtcccgc cctcaatctt tacaatcaca
      421 acctcatctc cggacttaaa ttcatccctt gtggattcat ctgctaccat ggctaatatt
      481 tgtatcaact gctcatcagt agttacggat attttgccct gactgttttt tgttactgaa
      541 acaagcacct ttgcattatg gccaactaac tgtgataagc tttttccctc cccaacctca
      601 gttcttttaa ggtagctgag ggccttatga ccaacaaacg ctgtaaacaa tcccataagt
      661 actgacgatg caactgttaa atactgttct gtctttaaat aggtaagcac agtgcccgta
      721 aaaccaaaga aggctacaaa gaatattatg tttcttagac ttagaaactt tacagcttct
      781 actgcacctt ctccatgagc atttgagcca atatccgaat caagatcagc atctatatcg
      841 acatccacat cagtatttat ttctatatcg gcatctgtat ccatacctgt aaaaatagat
      901 acgagcagaa aaataaatcc cagtataaaa caagctatat atactttttc catgatagtc
      961 agcgtttaat tggttttatt taaatccttt tttaatctgt ccagttcttc atttactgaa
     1021 ttttcttcag caagactttg cagatctaac tcttttttaa tttccagctc cagcacttct
     1081 ttttcagcct ctgcctgtga ttcaatcttt attatttcat tttcaattga atctacatct
     1141 gttattgatc tttcaatatc atcaataaag ctttttacat cagacactat ttccgatgat
     1201 ttttttgcag ccttctgtgt tctgagagcg gcctctgctg agcgtttcct tctgataagc
     1261 tcttcttttt ttcttcttac ttcctgagcc ctgtcttcaa gacgaaccag atcacttttt
     1321 agttgtgcag aaagatttac actactcttg tgttcttttg taagattgtc cctggtcttc
     1381 tcggtacgaa gcttcttttt taatacctct ctggccaatg cttcttcatc tttttccaga
     1441 gctagcctgg ctttactctc aagatcaatt atcaaatcga ctgtagaaga tagttgtttt
     1501 tcaagctgtt tttcattagc aacagcttta acagtttctc ttctaagctc aactatgctt
     1561 tcttctatat cccttataag ctgtttaacc ataacctcag ggtcctcagc ttcatcgagc
     1621 aaactgttta tgtttgccgt gattaatttt gaaattcttg caagcagttt catgtcatac
     1681 ctcctgacac tgtatttttt attgcaagaa tatatagcaa cagatatgcc agtggtaact
     1741 atctgtaatt attgatattt cggtttggcc ctggattata aaacaccaac cgatggtgaa
     1801 ttacaccaac aattcaccta ggttgaattt tttgtagtag tgtctgaact gatcataagt
     1861 catacccagt ttagccgcgg cttctctttg gtttccaaga cagtccttaa gtgtggttaa
     1921 tagaattgtt tttttaaatg cttttgttct gccataaaaa ctaccgtcta atggctccag
     1981 tgaggttatt tctattggca attccgatgc acatataact ccgtctatat ccgatatata
     2041 taacctctcg actatatttt tgagttccct gatgtttccc ggccagtgat attcttttaa
     2101 aacttttaca gctgccttgg taaaactctt tatctccaaa tcaggcattt cctgatggag
     2161 ctgatctata aagtgtttta gtaatagagg tatatcatcc cttctttttc taagtggtgg
     2221 aaccacgagt acagcaaacg agagcctgtc atataaatct ggaaggaact caccgttttc
     2281 catcatttct tcgagtggag cattggttgc ggcgatcact cttacatcaa cttctatttt
     2341 ctttgtaccg ccaagcctgt taaatgactg atactctatt acccttagta gtttttcctg
     2401 cacttttatc gacatgttgg caacttcgtc aagaaagaaa gtacctttgt ttgcaagctc
     2461 aaattttcca atcttggtac catctgcacc tgtaaaagcg cccttttcat ggccaaacat
     2521 atctgctatg aacaggtctt cctgaaatgc cgcacagttt acaataacat agggatactc
     2581 cgatcggtta ctaattgcat gaatataatg agcaagcagt tctttgcctg tacccctttc
     2641 ccctctaact agtataggtc tgggaacact ggcgtaggtt tcagcctgcc tgatgatatc
     2701 caaaaattca gagctgctgc cgactcctgg tttataatta ttatctgtcc cttgtatgtt
     2761 aacagacatt tactaccgac ccccaattaa acaatattaa tcatttttaa tataagtttc
     2821 aacagtaatt tttaaagacc ttaattacat ctatataaaa ataaactatt gatctagctg
     2881 tgctggctgt gatagaatga agaattggaa tatctaaatt agatctatat attaatggag
     2941 ttagggcatg gataacaaag aagtagacat ccttgagatg ttcaaacttt caaacaggta
     3001 cgatgttttt gaaccaggag atattatttt tgataaaggt tcagacggtg gaatcatgtt
     3061 tattatatta gaaggtgaaa tagaaataca ggttgacgga tttgcagtag gtaaattgtt
     3121 tgaaggacaa atactaggag agatggcact tgtagaagac cagccacgaa gcgccactgc
     3181 cattgctata acacatacta aaatagttcc aattaacaga gagacttttc tcttaatagt
     3241 aaaagaagaa ccgctgtttg cactctttgt aatgaaagac ctttcacata agatcagggt
     3301 tttaaaccag cgattaacta attgatcgca ctttgttatt atgtaatacc ttaactatat
     3361 tctgatctaa catagatttc ttacattctt cacattaatt caacttttct catttttact
     3421 atttactgaa tcctgagcgt atatatacag tctaaataca tatagtttga attggaggta
     3481 aaaatagatg tcatgcaata cagttgttca tgaagttgaa ggaaagcccg atcatcttat
     3541 gcagtttttg gatgattatg ataccgatac ggaacatgaa gcaataagca aacaacatga
     3601 tttgcttatt gagattcttg aaacttttcg cgcagacaaa agtgtaagaa atacggaata
     3661 ttaattagca ttgcagctta cgaaaagccc ttaaataaaa cagcgattga caaagtaaat
     3721 tcaatggtta ttatatacgt ataggaaata gaggcgtagt tccattctaa attaagattc
     3781 atttctaaat ttttttcaag agccaccccc gatgatccga gtttaattac aggaggtatc
     3841 ccattatgtc agaacgagta attggcacag taaagtggtt caatgcaagt aaaggctttg
     3901 gtttcataga gcgtgaaaac gaaaatgatg tattcgtaca ttatagtgaa attagcagtg
     3961 acggttttcg cagtcttgaa gaaggacaaa gagtagaatt tacagttgca gataatggcc
     4021 caaaaggccc acaagcacaa aatgtagttg cagtttaata tctaaaccat ttaaactaaa
     4081 acaaaaaaaa gcccggtttt tatgccgggc ttttttattt ctaaatcctc tattttactt
     4141 ttttaagcag ttctaaataa attctaacgc cttgtcctga actgactaac accgctagta
     4201 gtctgctcgg ttccatcttc agaagttgca actatctcaa agtagtagcg tgtgcttgac
     4261 tgaagtccgc taagaagcac cgtgtggtta gtatctgtaa caccatccgt ggcactttgt
     4321 gacagattgt tattgctagt accataattt accactacgg tcgaggggcc acttatagta
     4381 gacactacct cagcagatga tgaagtcttg ttttgtactc cgctatcaat aatagatagg
     4441 ctgcctgatg gtggaggtgt tggggttggt gttggatctc cgcctcctga acttctggaa
     4501 gttctaaact tctttgtggt agttgtctca gattgtccgt ctgcagagtc cacaacaatc
     4561 tgatagtggt accatgtgtt tgcgctaaga cccgtaagat ttattgtatg gtttgtatcc
     4621 gagacaccgt ctgttgcaga gttatttagt gaatttttgc taagtccgta atttacctgt
     4681 gcatttgtag gaatatttgt agatacattt acatcggctg atgaagaagt tttatttgtt
     4741 aggcctgagc ttgtgattgc aagcggggcc agattgttaa ccgtaactgc gatgctatcc
     4801 tcacccatgt ttggttccag atcataagcc ctagctgtga ttgcgcgctg tccgtttgta
     4861 aactgagtgc tgtcgaatgt acattgataa ggggtctgag ttagtgaggg gcaaacctct
     4921 accgcatcta caagcactgt gacaaaatcc actgtttcat tatcagtagc atttacctgt
     4981 actgtaacat ctcccgaaac ctcatcacca ttaagcggag aggtaatcga tacatccggt
     5041 ggtgtgatat ccggaggtaa attatccaca ttaatgtttg cgctgtcaga tgcactattg
     5101 tcgtttgcat catatgccca tgcagttact gtatgtgctc caaatgaata cagtgtagaa
     5161 tcaatgttgc agctgtatga aggctgatca cctgtaactg tacaataagg agtagcttct
     5221 gcatccatat aaaactctac tcggtcaata cccacattgt cacttgcatt tgctatggcg
     5281 ttaaagactc cggagacggt ctctccatct gttggtaagg taatatttac cgatggaggt
     5341 gttatatcag agccttcatc cagtgagaca attacatttg catgatcagc agtacggctg
     5401 tcatgtgtga tagtaatatt tcttgcagca tcagtaaaag tatctccaac cgcaaggttt
     5461 gcaacaagat aagaacggga gtttgatgaa cattttctat gtacgcttac cgtgttgcag
     5521 tattcagttc ttaaattagt atcaattcct tcgggctgtc tgtttgaaac ataaaaatac
     5581 tgtccgttta tatcctgtag aggaatcctt agcataagaa ttggatctgt aagcgtgccg
     5641 gggtctctat ttagtggatc gatttgataa gtacctgaag tctgagctat aggttttgca
     5701 gcagttggaa tccatgagta ctgatttttg tgaaatgcat ttacgtgaat atttccatat
     5761 ggatttccca aatcacagct tctgtctccg tattcactgt ttactgtaca atcgttatca
     5821 gtatctgttg aagcatggtg cattccataa ttatgtaaaa gttcgtgtgt cggcagaacc
     5881 ggccagttga gagagctgcc gtcataaaat gaatggcatg atgaagaact gcacccaata
     5941 tgcgcatatc cgtcccaacc gcatgatagc tccggaggaa gtatgaatgc cttgtgctga
     6001 tatgcatcaa catttacccc agcatcacgg gctgagttca taacggcatt acccatagat
     6061 acaaggtttg aaaaagcaca tggatttgat ggaaccggaa ctgaataagg accgaatacg
     6121 tcattatttg tatcgttatt aagatcgcag ttaagaccga gctgattgta tgagctgtct
     6181 ctccagacta catctgttga atctgatgca tcacacatat tattcatcat cggatcaggg
     6241 tttgctaccg tgtaacctgt accccaatct acaagaaatg tgattaccgt cctcatttca
     6301 gaaggatcgc ctgctgcctg tgctgatggg ccgtctgcaa tctgttctgt attttctgtt
     6361 ccgtctgcct gagaattagg gataatccct attatatttg caaaagcaga ttgagcctgt
     6421 gggggaagat tttctatatt gggatttttg gtaagtacga tgctggattt accacttagt
     6481 gaaccgccgg ttttaatttt gaccttgtca cctgtaataa gatcagaata tctgttatca
     6541 ggttcatttt tagaattttt tagtttgtaa acacccttac caggaatgat aatgctcttg
     6601 tcctcctgaa taaaagagtc aaagtcctct gcaatatatg cttcaacctc acccgttaag
     6661 accttcgagg ctgataatgg gtttgatgca aaaataacgc taagtagcat aagcgctata
     6721 aagctaaaaa ataaattggt tttcatctca atcctccttt aattataaac aaaacttcaa
     6781 caatcagatt aatactttag ttatagctta gtgaactcaa ctaaaccttt aatcgtatag
     6841 cgaattatac tatcgtctta tctaatagca aagtcttatt tctaaaagtg tcgtatttgc
     6901 tggcaattac aactaccatg tatataagca agtattatgc catctaaata ggcataatac
     6961 tccggcatta agggaaatta aacccgaaga tttttcggta gttagggaat taatttactc
     7021 tcaagaattt ttgaagtatg tagagagaat tgggtcttat aaaaaccact gcgtttatgt
     7081 gatattaaca cctgctgact caccctttgg attaggccca tattggtttt gacctggatc
     7141 actgtctttt acaagataat aaagcagcaa tattcctcct atgaaaggca caaatagtat
     7201 aagcaaccac caaccacttt tgcctacatc atgtacccta cgaatggaaa ctgctatgtt
     7261 gggtattaat gtagcaagaa tatataatcc accaatcaca cctggtcctt cgtcttttag
     7321 taatcctaat tgggaatcaa taaaagtaag cacaaaacat actattatat gaataagaga
     7381 aaatatccaa tattccttac gtctagccct accaccaaat acagcatatt tctttaaaac
     7441 ttcaatatac caattcatat tcccctccta ttatctttac gaaactatca taaagattag
     7501 atctatattt tgagtaatag ctggtgtcat gtcaaataat gttaactatc tgaatcaaca
     7561 acttcaaatt taggaatttg gtcatcctca tcatcagaag aaggaaggcc tttttgtttt
     7621 cttatgcctt caattttatt aagtggtctt tcgactgctc gttttcttgt ggtagttaat
     7681 acctcatatt ctttttgaac agcgtctatg cgttggccga gggtatcgaa cttagcaacg
     7741 aaagcatccc actgtttttt aaatgcacca aactgaacca gcatttcctt tgaggtctgc
     7801 tcgagggcaa agttgtctat tgcctgtctt atgattgcta gaacggcaaa gaagtttaca
     7861 ggtgagcata gcagaacctt attctgaatt gccgtatcaa aaatagccga atcctcttcc
     7921 tgtataaatg aatatatctg ctcgtttggg ataaagagaa tcacataatc aaccgtatta
     7981 ctttcagggc ttatataatc cttggtcgta acttccttaa gccttaattt aacatcattt
     8041 aaaaacgtct ttctataacc ttccttatcc ctttcagact gtgcctcaag ataatttaca
     8101 aagttattaa gtggaaactt aacatccata tttattgtta agtcctttgg aagcataaat
     8161 gtaaagtccg gtctctgcgt agagccttca agcgttttct gcttcacata gtttatattc
     8221 tcaacaaacc ctgcaagcct gagcacgtcc tctgccatcc tctctcccca ctgccctctc
     8281 gttttagtgc tggcaagagc ctcacggatg ctcccggtgg ttttcatgag ttcacttgtc
     8341 tgctgatttg tgatacgaag ctggtttgaa agctggccaa acttttccac cctgtccttc
     8401 tcaagttctt tcataagagt ggagaccttt tctagctcgc ctgtcatttt tccaagctgc
     8461 tgatctatta atcccttctt agtctcaagc tcctgtttag taaattctct ttcgggttca
     8521 agagtggact tagcaagagc aaggaactgc tctgttgatt tagaaagggc ttctaaagac
     8581 atactgccaa agctctcttt catgttcgag atgacctgat ctatattctc ctgtctctta
     8641 ctctcatttt cactgaatat ctcctcggca agctcctttg cattcttgcc ctgcattatt
     8701 tcaagaagat aagatacagc tacccctatc acgattccga ttataagtac agctatgtat
     8761 attaatgcag attcgggcat attctgttta attaatattt atatatattt ttataactac
     8821 ttcattatgg gtatatcaaa aatacttgat aggaataata taaaaaaagc ctgtattatc
     8881 catcctatta tacaaacacc tactgcacgc agggtactat catagtcaag cgcctgcctt
     8941 actgctataa tcatagttac aagcatccag actacagcaa tagaagtaat tatgccacct
     9001 acaacaggaa taaaataaaa tgctctaagc actcctggag aagaagagaa accaatagtt
     9061 ctcagaagct ccccatgatc tgctttagtc tgtggctgcg gcaataactt tgtgccgatt
     9121 atataagtaa tatatgccca tataaaccat ccggccaaag acaagataat aaaaataaca
     9181 atgccagata aatcaagttc tccgaacctt ccaagtcctg cggcaagact tgagataatt
     9241 acaacaacaa ctgcctgggt catagtagat ttatctgctt ctacctcttc atacaggtcc
     9301 acatcaagct tagccgctct tatcattctc tcaacaagtt tatccattct catttgtcct
     9361 cttcgctcat tttaacatct tgctgctctt ttgtctcttg gcacaacatc tttgtctttt
     9421 cccagtcgac attgggtagt agtttttctg attctatttg tttttgggca atcatcgtat
     9481 ttaatttctc tatcctgttt tctgtgttgg gatgagtaga caaatactcc gaaacctctg
     9541 ttgtaccatc atactccttt ttcattattt caaaaaaatc aaccatccct tttggatcaa
     9601 tgccggcttt aataagcatt ttcattcctt cagcatctga tgatgactcc tccgatcttt
     9661 tataagcaag gcttcttagc atattggcat aattcagagc tgtgcctacc acctgagaat
     9721 cacctatcat caagccaaga aaaataccga ttgaaagttc tttaatcagt atttttgtaa
     9781 aatgtctttg ctcaatatgt tgtatttcat gagcaaccac tccagcaacc tcttctggtc
     9841 tgttggactt ttctataagc ccccggaaaa gtatgatctc tcctccaggc agggcaaatg
     9901 catttacaag tgaattatta acaattgtaa tgttgtattt atatttacta ctgtctaatg
     9961 tggcaccaag tctgtcttta atcttatcaa tttgagtatt tagtcgttca tcttcacata
    10021 caccaaatcc tttacttatc tgtgaaataa aagatttgcc gatcttttcc tcccaggtaa
    10081 caggaaactt ttcaaccatt atattcatca gcggtggcac tatccatttg tgcaggagta
    10141 ctgctgatat aaaaagtgcg atgaaaacat aagcaacagt cttaatcctt ttatcagatc
    10201 catgtctgct aaaatttctg ccaaactgag gggatatact tcttagcgaa ccgataaaac
    10261 tcttatctct gattataagt atttccggca actcctgccc atattgcagt ctaacctcat
    10321 tttcagaata tatatcttta acctgtgtta ttcgaggata cagccacgtt atagtattgc
    10381 taaaagtatc agcatcaatc ctgagactgt ctcttgtaag agtaacttct acatcatgac
    10441 gccttgctga ccttccgtca taataaagac cttgaaattg aagcttataa gaactatcag
    10501 gggacatatt tatatattat atgccaagat ccataccaac caaatcaata tcgagaaaac
    10561 tggcaacttc ctcgccaagc gctgttggag ttctggcatc ctgctgtatg gctataagat
    10621 ccaaatcacc ctgaaggctc aggcgatcca gcaagaactt tatatttcgt atctgtacat
    10681 aaggatatgc aataccaagt gtaaatatga gcagaatata gtttactatc tgtaatttaa
    10741 cataatcttt ttcttcaata ttcagattaa acctggcaga tacaaatttg gtatgattcc
    10801 agtagtggtt gtgtctttga gcgaggaact taagaaagaa tataccccaa attataaata
    10861 acacgaagta gccgatatcc aggatatcgg taccaatcgt gggcataaat actgttagta
    10921 gctgatgagc gccagatatt agtattgcta ctaaccataa ttttgtatat ctgctgtaga
    10981 gatcatttat gttgccgtca aactcaaact ttgtgtctcc aaagtagata ttatttatca
    11041 gatattctct ggtcctatag tgaaaactgt aataataaaa tcctgcagtt aatgaagtaa
    11101 gaaaaaaccc tttaacatat agttttagaa actcctttgt attacctcta aacgaaaacc
    11161 ttactccgcg ccatgaagtc ctgctgtacc tgtatttgac tgtaaacaat atggcaagtg
    11221 gaattaacaa aacaatcaca atatatatga atattccacc taatatcttt gcaattacac
    11281 ctggcaccag atacataatt gcaaaggccg aacctatcac aacccctatg gctaatatgg
    11341 cctttataaa acccaggaat aactcgagcc cggtaccatg aaattcaaat ctgtccccat
    11401 caagatccgc ctgagagtag agataccttg cggctcttat tttcccccag aagaaataaa
    11461 taagcagagt tggtatcata agcaggatat ttacgatata tatgcccaat agtgtagtac
    11521 cattgccaaa gaaattaatt cttctataag agccgactcc actagaaaca ttaccagtac
    11581 tcccggcgtc cccaggcgca atgccttcag tatttataga agaacgaacc tgcgcagcgg
    11641 gtgtttgcaa attagggttt gtcatctgta ttggggtttc tgtaacatct aaaatctgtg
    11701 ttgagtcgcc gcctttataa cgttttctaa tagcctcact cgcttttagc atttcagctt
    11761 tttgttttgc aacaagactc tccgaatcaa ttatttgcgt ggcatctgaa tcagctctgg
    11821 tatcaatgga ttgtttcgct ttttgcattt cggcttgctg tttagccatc agatcacttg
    11881 tatctagaat ctgagtagca tcttcagata ctctttttgt agcagcactg tccgcagggg
    11941 ctggacgaga agttttttct ataaactttc tgcgtttttc ggttaatttt gcagactcta
    12001 catttttttt ataaagagga gtcgctacag gttgagaaga catgaggttg taaccgcacc
    12061 tgcaagtctt ccaactacct ggcactatag cattacagtt tggacatttc acttccttct
    12121 ctccatatat caaatgtgac tcttaacatt ttaatactta attgatacat ttatcaagta
    12181 gaattaatta atttataata aaccgatcaa aatagtttaa aaaagactac acttttatat
    12241 aatacaaatc ttaaatttaa ctataatcat caaaaataat ggaattaata aatcttatct
    12301 caattatcat ctccgtatca gctgcgttta gctacattaa ctacagattc ataaaacttc
    12361 ccacgaccat tggggtatta gtaatagcca ttatatgctc gcttggatta atcgctctta
    12421 gcagtcttgg atttattgag tttgagaaaa atgccaaact cataatatcg agcattgatt
    12481 ttgataaaac ccttctccat ggaatgctaa gctttttgct ctttgcaggc gcacttcatg
    12541 taaacataaa cgaccttgct aaacaaaaat gggttatcag cacccttgca agcataggtg
    12601 taatatcatc tacgttttta ataggcacac tgacgtggtt aatattaagc tttttaaata
    12661 taaatctgga ttttatttat tgcatagtct ttggagctct tatttccccc actgatccca
    12721 ttgcagttct gggtattcta aaaaaagcgg atattcctaa atcacttgaa accaaagtag
    12781 tgggtgaatc cctttttaat gatggaatgg gggtagttat atttctggtt ttatatgaaa
    12841 tagccttttc agggcatacg tttgatccat ctaacgcact ggttctattt cttactgaag
    12901 ccgtgggagg agcggtgttt ggttttgccg ccggatgggt agtttacaaa ctgatgcact
    12961 cggtagacaa ttatcatgta gaaatcctcc ttacgcttgc tcttgtaatg ggcgggtatt
    13021 cacttgccat gtaccttcat ctttcaggcc ctattgctat agtggttgcg ggtcttctta
    13081 taggaaacag ggggcgcatt cttgcaatgt ctgacaatac aagagaaaat ctggatacat
    13141 tctgggagct tatagatgaa attttaaatg taatattatt tgtgcttata gggcttgaga
    13201 ttttaatact gagtttttcc aagagctaca tttatgcatc acttctaatt attcccgtga
    13261 tactgctttg cagatttata gtagtatata ttcccgttag tatccttaag taccttagag
    13321 attttagccc aaacgcggtt aaaatcatga cttggggagc acttagggga ggaatttccg
    13381 ttgcgcttgc gctttctatt cccgctggag aacaaaggga aatcatagtt tcaattacgt
    13441 atatcgtagt agtattttca atcgtaattc agggacttac tataagcaag ttaacttaat
    13501 aaatctctta ttttacagga gtaggagtag gtgcaatatc ctttgtgatt tcaaaaccca
    13561 tttcatccat tgtcctttta agttttaaat aatcctcgag cgcttttagc tgcctctcat
    13621 ccagaaatgt ttgtgattta tctgttatta tctgatccag ctcttctttt tccagcatat
    13681 atctgttaat cctggccaag tcgacctcag aagctttgcc gctgttgaat tcatttgcct
    13741 gcaaacgttc tgtaaaatta aaatcctcag attcatttag tataagaaaa agcagatctt
    13801 cttcctggtc cagatcaaga aaaatctgtc tgtcagcaag gtaagtcttg aattctaata
    13861 tccaggatct gtactgtagc gtaagattat actcttcaaa taattcaaac tcatcatctt
    13921 caaaaaagct tcttaggttg ttatcggctc ttgctatcat ttcattgaat tttttctcat
    13981 tttctacaat ctgcgcctca ttcatatcac cctttagtaa agatactata atttcgagat
    14041 ttaccatctg tttatctact aaaagtccaa taagttcatt ttcttccgac tcatttaaaa
    14101 atagatcgac catgagatct tcataaattt catttacaac aatcttttgc tggtccctgt
    14161 agagctttct tccatttgga tcttcgataa atttaaaaaa atctttacca atttgatcag
    14221 aataaatctg atcggagatt atcatggtca aatcactaaa tttgtgctca agattttttt
    14281 gctggccttc gaggatatta acttgttcat taatctcggc ctttttttca tctatctgct
    14341 gatctagctt tgccaggtct gatctatact gtttcactct ttgattctgg taataaagta
    14401 aacaaccaaa aattatgaaa actataactg cggcaaatat ccctaagttc ttatagtcag
    14461 caccaagatt cttaaaaaat gttttaagat ttttcataat tattatgaat tgttattaag
    14521 ctcagactct gtcggaatga ttatccagcc aaaaatatat gcaattgtaa tcggaacaag
    14581 ggctgtgaca agacataaaa acacaagccc cagcctcact aaagtgggat caatatcaaa
    14641 catctcagca agaccaccgc aaacacctgc aaccatttta tttttttctg atcggtatag
    14701 ttttttcatt actttattaa tctattgtct tttgagtatt tagcctgctc atataatctg
    14761 cagacagcag accctctgca cacaggagat gagataaata ttcttttgtg ggaattaagt
    14821 cgtccgaggt tcttgtaggc tgtgctatat atacatatgc ttctttttca acaccatttt
    14881 ttaatatcac gctcattagt cttctgtcat attccacagg gtagctttca tatttatcca
    14941 gattaaatat gccattattg tctatttcat ataaaatacc ctgcacaagc tcattttcat
    15001 tctcaactat atttgcatgc cctgcaccac caaatgtata agtggtctta ttaaatctaa
    15061 gagtataacc aaatagacat gcttttgctc ttttaaagac ggttactcct ctttgctcca
    15121 tccgttttat gttcatattt gaaccatagg caaaatattt catttcttaa atttttataa
    15181 tatttttata aactacgccg caaggaaatc cgaatatgaa aaccgtgaga atcacaaaaa
    15241 ttccaggaat ctccaattgc cagaacaaaa tactaagctc aatcgaatta taattttgaa
    15301 aacaaattat tgtaataata catccaagaa ctattccacc tactaacttt aagctcataa
    15361 taagagatta cgataaagcc agatatcata ttataaatta tttaattaaa aaaatacaat
    15421 attttaaatt agatttaatt ataattgata aaattgtata ttaaccgcca aatgtattca
    15481 aaatagcttg ctgatatttc tgtactttac tagaatctat aaaatagtga ggggcactaa
    15541 gtatcggaag gtctattaat tgtttataat cattaatctt ctctctacac tcgtccgctg
    15601 tgccgactac agcaaacgtg tttaccatac tgtctgtaac agtgcttatc attgtaccta
    15661 tgtctctttt aaaatacgct tctcgtattt ttttgcattc tccttcaaat ccatgaagct
    15721 taaatggtgc ttcataggtt ttaacagttg cgtaaaaagc tattgtagct ttagctgctt
    15781 ctaaagcttc ttttttattt tcagaaatgg cacaggttat tatggatgca atagtaaaat
    15841 cttttctgtt ttttcgtgaa ttactaagac cattgttaat gctttggatt actacttttt
    15901 ttaaatattc ttgggagcat actacgtgac ccagatatcc atcggccacc tgccccgagg
    15961 cttcagtcat ttttgctcct actcctgcaa gatatatggg cacattttca cgaacaggat
    16021 tataagccct gtagtaacct tttgtgttta tatcaaagta ctccccttcg aacgtgaaat
    16081 cttctttttt gtgtgaacta ttaatcacat ttcttataat gtgaatacat tctttgattt
    16141 ttgtaacagg ttttccatgc tctacaccat gccacatttc atttgttctt ttcgccccgg
    16201 agccaagacc aagtatgagc cttccgccgc ttatttcatc aatatccatc gatgtgattg
    16261 cggtaataaa tggacttctt gtaaaggcaa gagctacggc tgtgccaagc ttgattttct
    16321 tggtagcatg cgccgctacg cttaactgct gaaatgaggt tctgtaaagt tcggtagccc
    16381 agacggaatg gaaattatgg tcttcagcct gtttggcaag gatggcaaga tccgaaatgt
    16441 tatgagcatc aagtatcaga cctatgccgt tcattatatt aggtaaacaa cccgtaataa
    16501 cttacaggtt ggatgtgcaa tagataagta taattactct ttcttatctg tagattcttc
    16561 ctcagccgct tgttgagagc tatcatcttc ttgtgcttta acttcaggct cagatgtctc
    16621 ttccacagac tgttcttctt gttcttgagg ttcagattta gcttcaggtt cttctacagc
    16681 tgcttgctca ggttcagctt ttgcttctgc ctctggttgt tcttcagtct cagattgctc
    16741 tgtagcctct tcagcagctg gttgcgcagt ttccggctct gctttcactt caggctctgg
    16801 agctgtttct gtctgtgtag cttcagtact ttgtgccttt gtttcttcta attgctcagt
    16861 tttctcttct gtatcgctca aacctagctc ttctgcagat tcttttttag tactttttac
    16921 ctcaggctct gcagctgcgg tttcagcctt tttcttcttg gctcgtttcg gtctcttctt
    16981 ctttggttta tactcttctt gtataagttc tattagtgaa ataggagcat tatctccagt
    17041 acgtactcca agtttaacaa ttcttgtgta accgcctggt ctgtctttga atcttgctgc
    17101 aagctcatca aaaagctttt gaatcacttc tttatccttt acaaacgcag cagctgcgcg
    17161 tcttgcagaa agagtgcctt tctttgcaat tgttatatat ttttctgcaa ctcttctaag
    17221 ctcttttgcc ctggtatcag ttgtttttat cttttcatgc ctaaacagat cggtaaccat
    17281 attcctaaac atagcccttc tgtgtttggt tgttactcca agctttctac caactctttt
    17341 gtgcctcatt ttatttctcg ctccagttta tatatataac tattgaatat tatgcgcttt
    17401 cttctgtctc aagcattttt tgtttttccg attcaaacgc ttctacatca ataacagaac
    17461 caagtgaaag ttccatctct tcaattcttt ctcttatttc aagaagtgac ctcttaccaa
    17521 agttttcaag atttagcaga tcatcttcgc ttttttgtat taattctcct acatatgcaa
    17581 tctcagcttt cctaagacag ttaagagatc ttgcagacag gtccatatct tcaatcgacg
    17641 taaacaaaat ctcctctgta ccgcttattt ccttatcatc ctctacctca attattgcat
    17701 caacttcttc ctcatcgaaa ttaataaaca cagttagctg ttcttttatt atctttgctg
    17761 cataagccac ggcatcttct ggaataaccg tgccatttgt ccatatttcg aggtttagtc
    17821 tttcataatc ggttcttcta ccaacccttg catttgtgat tgaatagtta acctttctta
    17881 caggagaata aattgcatcc agaggaataa gcccaataga ggtctgaata tctcccctgc
    17941 tctccacagg ttcatatcca taaccaactt ccacagtcat ttccatttcc agtttggacc
    18001 cttcggatag ggtagctata tggtggtccg gatttagtac atctacctga tgagtagtga
    18061 taatgtctcc tgcagtaaca tcacattcac cctgagcact aattgttatc gtctgttttt
    18121 caaaagtatg aaccttgagt tgcaaaccct taaggttaag aataatctga gtaacgtcct
    18181 cttttacacc cggaatggtt gaaaattcat gaagtactcc ctcaacctta attgatgtaa
    18241 tggcaggccc ctttatagac gatagcaaca cccttctaag cgcattaccc agagtgatgc
    18301 cataaccttt ttccagtggt tcacaaataa atctgccata atattctgta gacgatttct
    18361 cgtctatcac tattctctta ggtttaatta aatcctgcca attaagttga agatccttca
    18421 cgttccttcc tccatatctt ttttatttgt taggaagata aaattcaata ttatactctt
    18481 gaataaaact ccacaataag ctgctcttca atcggaatgg ttacatcctc tctctcaggc
    18541 aacctcttca ctgtccccat gagcttttcc tgatctattt ccagccactc aggaaatcct
    18601 cttctggcaa gcccctctaa agactgcaat attcttccga ccttattgct cttttcagat
    18661 acctgtattg tatcatttaa ctgtagttga taagaaggta tatctacctt acggccgtta
    18721 accataaaat gaccgtgtct taccataatc cttgcttctt ttctggagct tgcaaatcca
    18781 agtttataaa caacattatc aagtcgtctt tctagaagtg atatcaaaag gcttccagtt
    18841 acaccctttt ttcttgatgc tgtttcaaaa tatcggctaa actgtttttc agtaaggccg
    18901 tacattcttt taactttctg tttttctctg agcctgatgc cgtattcaga aaacttaggg
    18961 cgcctcagac catgatcccc cggtacctgc tcgggccttt tatcaacagc acatttttca
    19021 ctaaagcacc tgtcgccttt tagaaaaagc ttcattcctt cacgcctgca aaacctgcat
    19081 actgaaccta tatatctaga cactcttcct cggtcctcct tttgatatct gtaaacttcc
    19141 cataagctta aacacgtctt ttccccggag gtctacaacc attatgaggg attggtgtga
    19201 catcctttat cattgtaatt tttattcctg ccccctgaat cgacctcatg gcaggctctc
    19261 ttccaggacc cggtccttta atgtgtattg atactgattt aagccccatt gaggcggcct
    19321 tcttagcggc atcgtccgat gctatctgag cagcaaacgg agtacctttt cttgtaccct
    19381 taaaaccctt tgccccgccg cttgaccatg ccacaacatt accggcttta tcacttatac
    19441 tcactaccgt gttgttaaac gtagcctgaa tatgaactat tccttcttct agaaatttct
    19501 tagctttctt tgttgttttt ttcttagcca atgctgctac ccctccttat taacctttaa
    19561 ccttcttttt cttagcaata gcagttcctt ttctaccctt tctggttctt gcattagact
    19621 gtgtctgctg acctcttacc ggaaggttcg acctgtgcct cacccctctg taacaaccaa
    19681 tttcaacgag tctttttatg tttgtctgaa cctctcttct aaggtcacct tctactataa
    19741 attttgaatc aattatatct cttattcttg caatatcctg ttcggagaga tcggagcttt
    19801 ttgtactgaa atcaatctct gcttcagtta gaattttttt agaaatatcc ttgcccaccc
    19861 cataaatgta ctgaaggctt atctctattc tcttcttttc aggaatatct actccggcta
    19921 ttctaggcat aaaaaacttt cctcctaacc ctgtttctgt ttgtgtcttt tatttacaca
    19981 gatcactctt atagcaccct ttcttcttat cactttgcat ttatcacaaa tcttctttac
    20041 cgatgctcta attttcataa ttatctacct ccaaacttat ttcttaagtc tgtaagttat
    20101 tctgcccttt gttagatcat aaggcgaaat ctctaatttt actttatctc ctggcaatat
    20161 cctaataaat cttgttctca tcttacctga tacatatgct aatataatat gtccaccttc
    20221 aatttccact ctgaacatcg catttggcag agcttcaacc accgttccct caaattcaag
    20281 tctttcctct tttgccataa attaatgcac cccggactct gtaagtattt ccggtccgtt
    20341 ttctgtaatt gcaacagtat gttcaaaatg cgccgaaaga catccgtcaa ttgtaactgc
    20401 agtccagccg tcctttagta ttttaacatc cggactacct acatttacca tgggttcaat
    20461 tgcaattacc atacctgcct ttaattttcc tcccttgctc caaccactgt ttttgggaac
    20521 aaagtttggt acctgcgggt cttcatgaag acttgctcct atcccgtggc ccacaaatgt
    20581 tctaactacc gaatatccat tgctttctac atgcttttgt attgcttttg atacctcaaa
    20641 taaattattt tttggttttg cctgctcaat acccttgtat aaagcctctt ttgtgatcct
    20701 aagcaaattg actgcttcat cactaacaac tccaattgct gctgttattg ccgaatctcc
    20761 ataaaatccg tccttataaa taccaaagtc cattcctact atatcaccct cttctacaat
    20821 ctttttcttt gatggaattc cgtgaaccac ttcttgatta atcgcaaaac acacagagcc
    20881 ggggtaatca cgatatccct taaacgcagg ttttgcaccg ctttttatag ccatatcttc
    20941 agcaattcta tcaagatcat aagtagatat gcctggttgt gctttttcag aaagagcact
    21001 tagtacttct gcaacaatac tgcatgatga tctgatttta tctatttcct ctttgttttt
    21061 tagtacaatc ataaattact aacctattgt tccagctatg cgtgaaaaca cttcatctac
    21121 gcttccaatc ccatctactt cactgagttt tcctttttct ttataataat ccttaagcgg
    21181 ctctgtctga tttctgtata cttgcagtct gttctttacc acatcctcgg tgtcatcagc
    21241 ccttccttct tcttctgccc tttttagaat tcttgttatt atctcagagt cctgcacctc
    21301 taccgaaacc acagaatcaa tttccaaacc ttcgtttgag agcatggttt caagcgcatt
    21361 agcctgagca atcgtacgag ggaaaccatc cagcagaaac ccgccctggc agtcctgctc
    21421 ttttattctg tcctttataa ttcctattat tagttcgtct ggaacaagct cacccttatc
    21481 catgtattct tttgcctttt gtccaagctc cgtgccattt tttactgcac ctcttaaaat
    21541 atcaccggtg gaaatatggg caactccata tttttcaact atatttacag cctgagtacc
    21601 ttttcctgct cccggtggtc cgaatattat taaattcatt ttatctaaat atatctccta
    21661 atttttctct aaattaatat ctgcgtggcc ctttcatacc ttttttctta acaagccctt
    21721 catagctgtg gttaattaga aatgattcaa tctgctgtac cgtatccagg gtaaccccca
    21781 caacaatcag aagtgaagtg cctccaaaga aaaacggcac attaaatgga tcaagcctaa
    21841 gcacagaggg cataatacat acgacagcaa tatatatagc tccaataaag gtaagtctgc
    21901 ttaatatttt gtcaatatac tctgaagttt tcttccccgg tctcattccg ggaacaaacc
    21961 ccccgttttt cctcaggttt tctgcaaggt catcggggtt ataaattatt gctgtataga
    22021 aatatgcaaa gaagataatc agtgctgcat aaataaggtt ataaaataag ccgttatcaa
    22081 ggatcagtga agatatgtcc tgaagagtag ggatctcggc aaactgtaga atcgtagcag
    22141 gaaaaaacaa tagggatgaa gcaaaaatag gtgggataac ccctgcagtg ttaaccttta
    22201 gcggaagata agaagactgt ccgccatata cttttctgcc aactactctt cttgggtact
    22261 gaaccggtat ttttctaaat gatctttcaa caaacactat tgctgccaca atacatccaa
    22321 gaaatacaaa tagtattact gttccgaata tggtcatctc acccgttctg atgagtctta
    22381 caagatccca aaatgctccg ggaacggtag tgataattga tgcggcaatg ataagagaaa
    22441 taccatttcc aatacctctt tctgatatct gctcacctat ccacataaca aacatagaac
    22501 ctgcagtaag tgtaataatc gtagttaata caaaagaaaa accggggttt gtaactacgt
    22561 ttccaagctg accgctgtta agggtagctg caagcatacc tccctgcacc agacaaatga
    22621 gaatagttcc gtaccttgag tactgattaa tttttcttct accggcctct ccctcttttt
    22681 gcattgcttc aagtgtggga aacgccttta cgagaagaga cataataatg gatgaagtaa
    22741 tataaggcat tactccaaga gcaaagattg aagctctttc aagtgctccg ccagaaaaga
    22801 ggtttagcat atcaaagatt gttccgcttg cctgctggtt aaaaatatcc agtatttgtt
    22861 cggaatctac accgggaatg ggcacaaaaa ccccaagcct gtatacaaca agcataaaag
    22921 ctacaaataa aagcctttta ttcagctctg gaatcttggg aagcgatgat atacctgagt
    22981 tcatattatt tcaattgttc cgccctgctt ttcaattttt tcttttgcgc ttgcgctaaa
    23041 tccattggct ttaatgttaa gagatttgct gagatccccg tttccaagaa tctttatggg
    23101 gctattaccg cttacaagtc ccttctctct taaaatttcc ggtgttactt ctccgctttc
    23161 aaaaatatca agtctgccaa cattcactac atcaaattct actttattta tattagtaaa
    23221 tcctctttta ggagatctca ttttaagtgg agtctgtccg ccttcaaacc aaagtggtac
    23281 actacggcct gatctggaac gctgaccctt atgacctttt ccactggtct ttccgctcga
    23341 acccgggcct ctgccgattc tctttctttt cttctttgcc ccatcagttg gctttaattc
    23401 atttaacata agaaattctc cacataagtt taattgaata aacgaaatac cttgattctt
    23461 ttagattagt tacctggctg caggaatatc cagatcactt agctgcttat cccttatagc
    23521 agcaatttca tcagccatgc taagcgatga aagtccctgg attgttgcgt ttactacgtt
    23581 tataggatta gtagacccca ttacttttga taatatgtct ccaatacctg acaattcgat
    23641 aactgccctc accgcaccgc ctgcaataac acccgtacca gggcctgcag gttttagcat
    23701 tattctgcta gaactgtgct cacctataat ctcatgagga atagtgtgac cctttcttgg
    23761 aaccctaata agctttttct ttgctttatc tatagctttt ttaatagcat ttggtacttc
    23821 atttgatttt ccgattgcag accctattat gccgtcttcg ttgcctatga caacaagagc
    23881 tgtgaaatga aatctctttc caccttttgt tacctttgct actcttcgga tatgaactat
    23941 tttctcttta aggtccagcc catttgggtt tattttctca ttgctcaata atctattctc
    24001 ctcacttatt aaaacttttt attaaaattt gatccccttt tcccttacag cctcagccaa
    24061 ggatttaacc cttcctgtaa agggaaaacc acctctgtca aactttatcg tatttatacc
    24121 tttttcttta catctgtctc caagttcctg ccctaccagt ttagaagctt cggctttacc
    24181 gagcttatct ttctttatca gttccgagat tttcggtgta agtgtagaac atgaaaccaa
    24241 cgtgttttga gtcgtatcat caattacctg ggcataaata tgtgaaagtg atttaaacac
    24301 tgaaagtctt ggggtttgag aattacccgc aactttcttc ctcacacgtc tgtgcctgag
    24361 gtttttcata ctctttctgc tttttacttt tatcattaat taagctcccg tcttagcgcc
    24421 tgattttcca ggttttaacc ttggctgctc cccttgatat ctaacaccct tacctttata
    24481 tgcatcaggt actcttaact gtcttatctt tgcagcagtc tgacctacag cttctttatc
    24541 aataccgcta agaatcaccc tggttcccct ttcttcaata accgaggaga taccttctgg
    24601 aagtacgaag tctattggat tagaataacc aaggctaaat tttataccct gcttgccggc
    24661 ttcttcagcc ttataaccta ctcctacaat ttcgagtgtt ttagtaaaac cctcagaaac
    24721 tccggttacc atatttgcaa taagtgacct tgtgagtcca tgaagtgcct tggccttgtt
    24781 atcatcactg cttctctcaa atattaattt accatcttct agctttattt taataagatc
    24841 ggaataagta cgcgaaagat tacccttggg gccagttact tttactatgc cctgtttaaa
    24901 ttcgacttta acgttatcag ctacggttat aggattattt ccaattctag acataaaaac
    24961 ctctcattac attactgtac atataaactc tccgccaagc ttttgctccc ttgcagcccg
    25021 tccagccatt atcccttttg atgtagagat gattgatgtt ccaagcccgc cgcttactct
    25081 tggtatttgc gtacttccta catatactct cctgctgggc ttgctaactc ttgtaagttt
    25141 cttaacaacg gactcttttt ttgaaccgtg atatttaagc tcaacatcta aatatttaaa
    25201 cttattttcc tgcacttctt tcacttctct aatataccct tcatccttaa gcactcttag
    25261 tatttcactt tttatcttag agtctggaat tctcacgcta gggtgtcctg cgataagagc
    25321 atttctaatt ctcgacaaca tatcagatat aggatcagtc atataatttt aaacctcttt
    25381 ttattctaat agcctttacc agctggcttt tcttactcct ggaagcttgc caaaacttgc
    25441 aagttttcta aaacaaagtc tgcacattcc aaactttctc ataaaagacc tgggtcttcc
    25501 gcaaagcgga cacctgtttt tgacccttac tttatattta ggctctttat tcgatttttc
    25561 aattaatgct tttcttgtca tatgtccctc ttgtttactg ttttacaagc ggcatcccca
    25621 tgccaattag aagttctttt gcctgttcat catttatggc agttgttata attgatatat
    25681 tcataccttt tgttttttct actttattgt aatcgatttc aggaaaaatg gtctgatccc
    25741 taagacccag ggtgtagtta ccattaccat caaatgcttt agctgatgtc cccctaaaat
    25801 cccttaccct tggaagcgct atactaacaa gcctgtcgaa aaactcatac attcttgtac
    25861 ccctcagtgt aaccatacat ccaactgctg aattttccct aagtttaaaa cctgctatag
    25921 aattctttgc tcttgtgata actgctttct gaccaacaat taaagaaagc tccgatgccg
    25981 cgtcttcaat gagtttataa tcctttcccg cttcgcttag ttttccaagt cccatgttta
    26041 ggactatctt atgcagctta ggcacctgca tgatattgtt atagttaaat ttttctttta
    26101 gctgaggtat aacctgctgc tcataatgag tttttaatct tgctgacatt agtctttaaa
    26161 ctcctcttca catcttttgc agtaccttat tttttctcca ttctcaagca ctttattacc
    26221 tgcccttgat ggcttggcac attttttaca atataaaagc atatttgaaa ccggtattcc
    26281 tacctcaacc tcattaattc ctccggttgg acttttctga gtaggttttg tatgtttttt
    26341 agctatattt agtttttcaa ctacagcctt attcttaacc ctgtctattt ttagcacttt
    26401 gccggttttg cccttttcct tacccgctat aacataaaca gtatctccgg tctttatatg
    26461 agtgctattc ttttttattg cattagtctt caatctaaac aacctccggc gcaagtgaaa
    26521 taattttcat aaatcctttg tttctaagct ctcttgcaat tggtccaaac actcgggttc
    26581 caatcggctc catatctttt gttataagca ctgctgcatt atcatcaaac ctcacatacg
    26641 agccgtcctc tctcctctgt tcttttcgtg ttcttacaac tactgctttt tgtattgatc
    26701 ccttttctac cttggaatta ggaatcgctt cttttacaga aactactatc aaatcaccta
    26761 ttcttgcata ctttctcgat gaacctccaa gtacttttat acaaaagagg ttttttgcgc
    26821 cgctgttatc ggccgaatcc aaaactgtgt ggtgctgtat cattgatcct ctcctgcaac
    26881 tactgcagct tcgtctttaa tttgcaaatc tggtacaacc ttttgttgaa ttattttgct
    26941 aacgcgccat ctctttgttt tactgattgg ttttgattca gtgatctgca ctttatcacc
    27001 cacgctgcac ttattttcct tatcatgcac cttgtatgta gaagatcttt taataggctt
    27061 attatatact ttgtgtttta ccgtgcttac gatttcaaca acaacagttt tatccatctt
    27121 gttgcttttt actacgccta ttctcgttct tgcttttgct cttgccaatt tattactcct
    27181 tcgctccttt gagctcgttc tctctttgaa cagtgtttaa tctggctatc agctttctca
    27241 ctctgccaac ctcggacact ttcgtatttt gctgtgttga aacctgtacc cttatgttaa
    27301 aaagctcatt tttagattct tcaagctttt ttgcaatctg atcactgctt aaatttctga
    27361 tttcacttat tttcatctta tacttatccc ttcttcacgt cttaccattc tggttgctag
    27421 tggcagcttg tgtgatgcca gcctgaatgc ttcttttgca agctcatcat ttagaccgcc
    27481 aatctcataa aggattgtcc ccctttttat gggtgctaca tatccgtcta catctccctt
    27541 acctttaccc attctggtct cagcaggcct tttagtgatt ggtttgtgtg ggaaaattct
    27601 tatccacagc ttagcacctc ttctcacatg ccttgtgatc gcaattctag cagattctat
    27661 ctgcctcgaa gtaatcatgc cgttttcggt tgcctgaaga ccaaaatctc caaatgcaag
    27721 gtaatcccct tttgtagcct tgcctctaag tctaccttta aactgttttc tgtatttagt
    27781 tcttgatggt tgtaacattt aatactccat ttatgaagct acctgtggct gctgcctgct
    27841 agtttttttc tctaatactt cacctttaaa aatccatact tttataccaa taattccata
    27901 cgttgttttt gcttcggcaa ggccataatc gatgtcggcc ctgagagttg taaggggaac
    27961 ccttccttct ctataccatt ctttacgggc aatttctgct cctccgagcc ttccggaaac
    28021 cattatcttt ataccaattg caccaagtct ctgcgaggat gacatggaac gtttcatggc
    28081 ccttctgtaa gctacccttc tttgaagctg aagtgctacg ttttctgcta caagctgagc
    28141 atcgagctca ggtcttttga cttctcttat atctagatag atgtccttac ctgtcatact
    28201 tcccaggcgt tttctaagag tctcgatttc ctgacccttt cttccaatta tcataccggg
    28261 tcttgaacag aaaataacta ctcttaccct ttcagatgct gccctttcaa gctcaatctt
    28321 tgaaattcca gcctgataga actggtcttt tataaccttt ctgattttaa tatcttcgtg
    28381 aagaagccta gtgtactttt gcttttcagc aaaccacttg gaatcccaac tcttagttat
    28441 ccctattcta agtcctatcg gacatacttt ctgacccaaa ctataacccc ctctgctcta
    28501 atacaatttt tatatgactc attttctttt ttatactaaa tgctctgccc atggctcttg
    28561 gccttatacg cttgataaaa gggcctttat taacaaacac ctctttaata tacaggctgt
    28621 cagtatcact atgtcctttt tccgacgcat ttgccactgc cgattttaag agcttggata
    28681 ttatctttgc agcttttctg tttataagcg aaagagttgc aaaactttcc tcaacacccc
    28741 ttccccggat ctcatccaat acgatcttag tctttttatg tgacaaccta gcaaatttta
    28801 atgtagcgct tgataccatg acaattatct tatcctatat aaattatttt ctgcctttac
    28861 cctttctatc cccaccggca tgactataaa atgttcgtgt aggagaaaat tctccaagct
    28921 tatgagcaac catgtgctct gttacgtata ccggaatgaa tttcctgccg ttgtacactg
    28981 caaacgtaag ccctatcata tctggcgtga tcatagacga tctagaccag gttctgataa
    29041 ctttctgatc attcgtttct ttggctttat caactttctt ttgcagttta gggtctacaa
    29101 acggcccttt tttactcgat cttcccataa tttctcctaa attaatccat tccgtaacct
    29161 attcttcttc tcttcaatat atatttatca gttcgtttat tatttctggt tttatatcca
    29221 attgtaatcc aaccccaggg tgatacagga tgtgggttac cctttgttgc tctaccttct
    29281 cctccgccat gcgggtgatc cacagggttc atcgcaacac ctcttactac aggccttttg
    29341 cctctatatc tctttcttcc cgcctttcca agcactataa gctcatgatc ggtgttaccc
    29401 actcttccga ttgtagccct gcagtccaaa ggtacaagcc taatctctcc ggatgaaagc
    29461 ttaatctgag catatttttg ctcctttgcc ataatctgcg cagatgttcc tgcagatctt
    29521 gaaagcttgg ctccgtttcc gggtttaagt tctatattat gaactgttgt accatctggt
    29581 attcttctaa gaggcaggct gttacccgtt ttgatttcaa catcattgcc gcttacaatt
    29641 tcatcaccaa cctttaatcc atcaggtgca gtgatgtatc ttttctcacc gtctttatat
    29701 accaaaagag ctattcttga tgttctgtag ggatcgtatt caatggtgct tacctttgca
    29761 ggtatatcaa tcttatccct tttgaaatct attattctgt aaagcttttt attccctcca
    29821 cctctgtgcc acaccgaaat tcttccttcg gagtttctgc cgccagtttt cttaagctta
    29881 atcgtaagtg gtttatggct ttttttagaa gaaatatccg aataatcaga taccgtcata
    29941 aaccttcttc cagcagatgt agggttatat ttctttattc ccattattat acaccttcaa
    30001 aaaattcaat atttccttct tttaatttta cgtatgcttt tttaaccgaa gaagtctttc
    30061 ctattgctct tccgtatttc ttaactgttt ttcccggact gatagaagtt cttaccctat
    30121 ccaccttaac tttaaacagc ttttctactg cgttttttat ctcaatcttg tttgattttt
    30181 tatcaacaga aaacagatac catcccgcct cttttaccat cgatgatttt tcagttatta
    30241 aaggtttttt tattacttga gtaggatggt tcaatgtgct agcacctctt ggactttagt
    30301 taatgaatca accgtgaaaa ctatattttt aaattttagt atatcgtaaa cattcaggcc
    30361 ttctggtttt aggactttta cgtttgagag gttcttggat gattttttaa gaacttcata
    30421 attgcctgat tctattagaa gggttgagtt aatatcaaat ttatccaata ttgcagaaac
    30481 gtttttagtc ttgatttctg atgccttaaa atcatccagc actataacac cgttttcact
    30541 gttagcatgt gaaagtgccg acaacagcgc ttttcttcta actttctttg gaagtgaaaa
    30601 tgaccagtcc tttggtttag ggccaaaggt tactccaccc tttctccata taggtgacct
    30661 gttactacca gaccttgctc tgcccatacc cttttgcttc cagggtttag aacctccacc
    30721 actcacttca cctcttgttt tggtgcaggc tgtacctgac ctcttttttg caagctgcca
    30781 gttaataacc ctatatataa tatcgcggtt aaccggagct tcgaacacag tcgttgccag
    30841 gtcaatcgta tttacttttt tcttattaat atcgaatacg tctgccattt tatctaattc
    30901 cttaaactct tccctttaca gtgtgtttta agaatatgtg tgatccatta ggcccgggaa
    30961 ctgaaccttt tacaagaaca aggttattct caatgtctac ttttactacc ttaagaccct
    31021 gaatcgtaaa cttccttgct ccataatgac ccggcatttt ctgccctttc caaacttttg
    31081 caggaaagga agcctgacct attgaaccca ctcttctgtg tgccataccg ccatgtgaat
    31141 ctctctgacc actaaatcgg tgcctcttca tagcacctga aaagccttta cccttactta
    31201 ctgatgtaac atcaaggtaa tcaccttcag caaatatatc tgcagaaact acgtctccgg
    31261 cattatattt atccgagtca ttatttagta ctttaaattc ttttagcaga ccaagcggcg
    31321 gtactccggc ttttttaaac actccggaca tcgctttatt ggacctttgc ggctttatct
    31381 ctgcaaaacc tagctgtaca gaatcatagc catccctttt ctcggtcttt ttatcgacca
    31441 cataacaagg ccctgcttca attatagtag tgggtataac ttctcctgtt tccaagaaca
    31501 gctcggtcat ccctttcttt ttacctatta gaccttctat cattacctgt taattccctg
    31561 ttaatttaat ttctacttct actccgcttg ccagatccag tttcataaga gcatcgactg
    31621 tttgctgcat tggctctaga atatctacaa gcctcttatg cgttctgatt tcaaaatgtt
    31681 ctcttgagtt tttatctaca tgcggagacc tcagcacaca gtacctgcta atcctagtag
    31741 gtagtggaac tggccctgct attctggcac cggttctttt aaccgtgtct actatctcta
    31801 aagtagattt atcaagaagc ttatgatcaa atgattttaa tttaattcgt atttttggaa
    31861 ctcgactcat aataattttt tatcattact ccaatatttt agttaccaca ccggcaccta
    31921 ctgttctgcc gccttctctt attgcaaacc tcagcttctc ttccattgcc acttctgtta
    31981 ttaactccac aaccatgttg a
//
LOCUS       DBNGSV010000003        15955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1662466, whole genome shotgun sequence.
ACCESSION   DBNGSV010000003 DBNGSV010000000
VERSION     DBNGSV010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1662466"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /gene="ribF"
                     /locus_tag="AC159U_00275"
     CDS             complement(<1..667)
                     /gene="ribF"
                     /locus_tag="AC159U_00275"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIR3335524.1"
                     /translation="MQLLIDPKENIGKSVCATIGIFDGVHLGHKSIIDQVKKEARAKG
                     LSSCVITFDPHPQEVLTGQKLPFITPFEKRVKLFEKEDIDIAVCFTFDKEFSKITAEQ
                     FIRDTLVNVLNLKEIFIGHDFVFGSKRGGNYELLKKMGNELGFKATIIDPVTNGSNVI
                     SSTAIRNLILEGHISKANKYLGRNFSLEGTVTEGEKRGRLLGFPTANLKTDWELLPKP
                     GVYI"
     gene            complement(670..2061)
                     /locus_tag="AC159U_00280"
     CDS             complement(670..2061)
                     /locus_tag="AC159U_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3335525.1"
                     /translation="MLIPAFCSIIYKEDDIYSFLISGVITFLVGISGEQLTKSEADID
                     EFNRKDGFFIASFCWISAIAFGSMPYLIYGVFSNPMDAFFESTAGFTTTGASTVVNIE
                     ILPHGILFWRSFTQWLGGMGILILAIAILPKLSVGGMQLLNIEAPGPSAEKLTPRIAE
                     TAKHLWTVYLVLSILLFVILNLLGLPIFDSIIHVFSTVSIGGFSNQNLSVGAYNSVAV
                     EFTITAFMFISGINFALLFWLSRGKFKKLYSNPEFRFFVIIIVVSITLVTIDLYTYQF
                     SNLFESFRYAAFNVTSISTTTGFVSTDYNLWPEFSKWLLIILMFVGGCSGSTSGSIKV
                     MRIIILIKHAFREIKKNIYPNAVLPIRFGKNVLDDNVVSSIINFFLIYIFIFILSTII
                     LTLDEVPIITAFTACAATIGNVGPGMDLVGPASNYAGLSNLSKTVLSFLMIIGRLELF
                     AILVIFTPSFWKN"
     gene            complement(2121..3443)
                     /gene="trkA"
                     /locus_tag="AC159U_00285"
     CDS             complement(2121..3443)
                     /gene="trkA"
                     /locus_tag="AC159U_00285"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR3335526.1"
                     /translation="MRIVIIGSGEVGTFLANNLSDEHDIVIIEKNKQQTEYAKEHLDA
                     LIIHGEGDNPNVLKQAEIEKADIMLAVSGNDRTNILASIISHSLGIERIIARIRDPDY
                     FEYPQVIQIPQITVVNSGLLVAEKIANLISTPFAWKTETFASGKIRMLKLRIEDNSLI
                     EGKKLSEMDPPQAWIFVAISRDGEISIPTGDTRLKKGDYVFALGIPSISDKLEALLGV
                     KREKVDMAIISGGGRVGRRVARTLIENGIEVRVIESDHERAELIAEELPEVMVFDGDA
                     TNKEILKEAGAAHADYYVALTGDDEKNVLSALLAKNLGTKRSTVLYTKPDYIDLIESI
                     GVDRAISVRLSTANEILSMLHLKGVAHVALIEEGKAEILEFMIHEHSKILGVPLHKAG
                     FPKDSIVGIVVRGEEIIIPRGGYVSEIGDMLIIFCLPGAVKTVEKILS"
     gene            complement(3459..4220)
                     /locus_tag="AC159U_00290"
     CDS             complement(3459..4220)
                     /locus_tag="AC159U_00290"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3335527.1"
                     /translation="MYPTLFQIGSITISSYSVMVTLAYLAAYIFLSLEIKRRGLDDSI
                     ADWVLLAALFGGLGGAKILFLFQNATFAEFMNEPLKFLSSGLTFLGGLIGASILIFVV
                     GIWKKISFWTLTDSTAPGLVLAYGIGRIGCLLVGDDYGTPTSVPWALSFPNGSPPTTQ
                     LVHPTQIYDTILMIFIFLILWAIRKSDFPTGWMFSLTLIILGIQRFFIEFIRNTSPSF
                     IDGLSQAQLISIVIFIFGVLNLLRLKFMLNKPAIT"
     gene            complement(4344..6047)
                     /gene="recJ"
                     /locus_tag="AC159U_00295"
     CDS             complement(4344..6047)
                     /gene="recJ"
                     /locus_tag="AC159U_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3335528.1"
                     /translation="MKTRLIIQKENQKLSEKISKTLEISPITAQLLINRGINTAEEAH
                     QFLNSSLFDLPSPFLMHGMEKAVKRIIQAIENMEIIGIYGDYDVDGVTSTALLYNFLK
                     EIGSRVTYYNPDRFTEGYGVNIEAIKKLDKQGVKLIISGDCGITAVQEVEKARELGID
                     FIVTDHHKPPDQIPDAVAVLNPQLGQCNYPAKEITGVGVIFNLVLALRRELRESGFFE
                     GKEPNLGAYLDLVALGTVADCGSITNVNRILVKEGLKRMGSSKRQGLIALKEASSISG
                     EVSSFDIGYRLGPRINASGRLNTAKEAVELFISNNPQTAKELAGLLNTENTNRQGIEA
                     KILKEAFSMIDSDPKIKDSNSIVLASRNWHQGVIGIVASRIVDKYLKPTFLIAIDDTG
                     MGKGSGRSLEGVNIYTALTKCAGCFEEFGGHDLAAGIAIKEQNIDQFKLQFDKALKGF
                     KDKYVKKIIVDAQIETHDLSKELIMEFENLAPHGIGNPEPLFFTKSLEVVDRKIFKEK
                     HLSLKLKNTTKSYDAVWFNCKLQLEPANKIDIVYTPEINRWNGSNNIRLRIVDASKHE
                     S"
     gene            complement(6058..6498)
                     /gene="aroQ"
                     /locus_tag="AC159U_00300"
     CDS             complement(6058..6498)
                     /gene="aroQ"
                     /locus_tag="AC159U_00300"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3335529.1"
                     /translation="MNILIIHGPNLNMLGKREPDIYGSTTLSDINNLIEIVAKSLNVK
                     TEYFQSNSESEIVTKIQNSLGNIDGIVINPAAYTHTSVAIRDAILSTDIPTVEVHISN
                     IYKREKFRQNSYVSGVSLGVISGFGTYSYILGLKAITNHLKSKK"
     gene            complement(6500..7195)
                     /locus_tag="AC159U_00305"
     CDS             complement(6500..7195)
                     /locus_tag="AC159U_00305"
                     /inference="COORDINATES: protein motif:HMM:NF020855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3335530.1"
                     /translation="MNTETDSLNNLLESLIERYAQDPNSDLILAGISKSQGDIDKALS
                     ILFAALRKNQNDISAKLMIAEIYYERWMIDEAKKYLEQLIDQTPNNEKALDMLSKIYN
                     SENDSSKALDILYKKLIFCANKSQTLYEIERLKNKSLTQSSHFDITKNIKNKLSDSSQ
                     NVKHTTREVNLITETMADLYINQGYYEKAAEILEQLVKKYPHDPHLKTKFSLSMASKS
                     ISKKSFDASEELD"
     gene            complement(7236..7505)
                     /locus_tag="AC159U_00310"
     CDS             complement(7236..7505)
                     /locus_tag="AC159U_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335531.1"
                     /translation="MEEILDDVVIKLPVHKRLWKWWVPRAHRIGNFQARLILTIFYFT
                     MFMPFAILVRLFTDPLRIKPKTEKGWIDRNNEPVDDLLARARKQF"
     gene            complement(7586..8887)
                     /locus_tag="AC159U_00315"
     CDS             complement(7586..8887)
                     /locus_tag="AC159U_00315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3335532.1"
                     /translation="MNICMIGTGYVGLVTGACFAGSGNNVACVDIDEEKVKSLSKGKV
                     TFFEPGLEELVKENFDEGRLTFTTDLKSALDKSEIVFIAVGTPPKEDGSADLSFVRNV
                     AESIGKNMTDYKIIVTKSTVPVGTSEMIRDIVKKQTDIDFDVASNPEFLKEGAAVEDF
                     MKPDRVVIGVDNEQVGEKLKQLYEPFMRSSDRTQIVSIRSSELAKYASNAMLATRISF
                     MNEVANLCERIGADVSDVRKAMGSDKRIGTAFLFPGVGYGGSCFPKDVKALIQTAEES
                     GYKLKVCSAVDEANQYQREIFWQKINNYFNGNLKNKKIAVWGISFKPNTDDIREAPAL
                     YIIEKLLKNGAKISAHDPVATENAEKELGGSIEFVEHNYDACEGADALVIHTEWNQYR
                     QPDFNKLKEIMNSPVIFDGRNLYSPSKLKEVGFEYFPFGRT"
     gene            complement(8981..10534)
                     /gene="murJ"
                     /locus_tag="AC159U_00320"
     CDS             complement(8981..10534)
                     /gene="murJ"
                     /locus_tag="AC159U_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3335533.1"
                     /translation="MTDKKTIAKSASSVSFFTFISRILGLVRDIVITSLFGAGQVTDA
                     FWVAFRIPNLLRRLFAEGTLTVSFIPVFTERLEYGGKEKAKVISDSIFTILVCLLVII
                     SLLGIIFSPYIVKIFASGFSDDVFDLAVKLNRIMFPYILFISITALSMGILNSLKHFF
                     APAFSPVLFNICIILFALLLHDQFDIPIITLAIGVIAGGLLQLFFQIPFLKSRNFLFN
                     PKLVLNNPDVRKIFSLIIPQVFGLAVYNLNIIVNTQYASYLPEGTVSYLFLSERLIEF
                     PLGIIAVSLATVLLPNFSSYIAKGDLNKFRETYLSSIRILFYILVPACIGLIALSKPI
                     CNILYQRGEFGYEQMVFTSQCLFGYALGMWAVGLIRITVPAFFAQKDTKTPVYIAFTA
                     FILNAVLGYVLSFKLELNHIGLALSSSASSVLNISLLIYLINKKIVNIEIASLVGYMS
                     KIILIGTIMGISVYQLSLFTDWTGSNFDINKVGALGVCLIAGIVIYSGLSKILKIEEF
                     DKITSMIKK"
     gene            complement(10527..10772)
                     /locus_tag="AC159U_00325"
     CDS             complement(10527..10772)
                     /locus_tag="AC159U_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335534.1"
                     /translation="MPQILIKIKNKSDTVIFHSLLGTYSHLAWVRTEIPDQDIISVTP
                     TDDMLEETMQVLDNLKQEIEFEVLEVKHLDKYGLQND"
     gene            10866..11720
                     /gene="htpX"
                     /locus_tag="AC159U_00330"
     CDS             10866..11720
                     /gene="htpX"
                     /locus_tag="AC159U_00330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR3335535.1"
                     /translation="MNTLKTLILLASLSALIIWMGGAIGGSTGATIAVVIALAMNFAS
                     YWWSDKIVLSMYKAKEVTPEQAPELYNATLELAQNAGLPMPKLYIIPEDTPNAFATGR
                     NHNHSAVAVTSGIMRILNMDELKGVIAHELAHIKHRDILISSIAATISTAITYLSYMA
                     MFFGGGRDDNRGGNPIALIAMMILAPLAATIIRMALSRTREFLADKGGAEICGNPLHL
                     AGALRKLGEYSGRSQLQVSEQVADSTAHMFIVNPLLGKGLAALFSTHPPMEQRIEKLE
                     DMAAGIVR"
     gene            11727..12056
                     /gene="hspQ"
                     /locus_tag="AC159U_00335"
     CDS             11727..12056
                     /gene="hspQ"
                     /locus_tag="AC159U_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HIR3335536.1"
                     /translation="MNEIDKTIEGKFYIGQVIQHKLFDYRGVIVDVDSKFEGSEEWYE
                     AVAQSRPPKEKPWYHILPDGSAHKTYVAERNIENDKSGKPIEHPAVGAFFSGFKDGTY
                     ITNKGFN"
     gene            complement(12084..12683)
                     /locus_tag="AC159U_00340"
     CDS             complement(12084..12683)
                     /locus_tag="AC159U_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335537.1"
                     /translation="MTFIERIARKSLDTLDNVRTSATTSRRSRNSESDQQEYDMELDV
                     CCNGHTFKFLNSQTDSENNYTKTSFIVEGYIYKAGTFENFGYDKGVKTNGDPEFENLL
                     IGKWTNKGWYLNKGRQTKTGPYVDSTHTYDLTEGNSLKKILSSNGKELIDMNTPFNRI
                     LSAGQGGYSRIRGGNVIQINVGKNDTLNFNYTFRFNFRN"
     regulatory      complement(12771..12875)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(12956..13321)
                     /locus_tag="AC159U_00345"
     CDS             complement(12956..13321)
                     /locus_tag="AC159U_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335538.1"
                     /translation="MISYETYKVLHVAAVFFLLFSLSTYLIVINSNIEKFKKLAGIMH
                     GISMFIVLLGGFGLLARLGIIHGMDWPPWVWGKLVIWVIMGGMTFFIKKLQPQAAILW
                     FTVPILAIIAVYFAVYKPM"
     gene            complement(13338..15344)
                     /locus_tag="AC159U_00350"
     CDS             complement(13338..15344)
                     /locus_tag="AC159U_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3335539.1"
                     /translation="MIAIKNLSLSFGSYKILNGLNWHIKDCKKIGLVGPNGAGKTTLL
                     ELITGSLTADEGSVSLPSSSVIGYLPQQFALNNAQTSLLEETLTVFKDIKQLEQDNEQ
                     LLHDLSKYKDETTPEYKLLLKKIDSIQNELKIQNSETVNKRAEKMLMGLGFTVNDLSS
                     PLSTFSGGWRMRVELAKLLLQNPEFLFLDEPTNHLDIESIKWLEDYLKSFEGSVIVVS
                     HDKYFLDRMTEITAELSGGKITEYQGNYSKYLELRMRNVEIQKSAYENQQRQIKQYER
                     FIERFRYKNTKAKQVQSRIKMLDKLERIEETGTDKSVNFRFNNTKRSGKNVLEISAFS
                     KSYGSNEVFKDCDPLTILRGDKIALIGKNGIGKSTLVRIINGTEKFDGQRKTGYEVEL
                     TYFAQNQAKTLKPEYNIFEELAHSSDSKVSNETDIRTILGNFLFSGDDVFKKIKVLSG
                     GEKSRVALAKTLLSPKNFLILDEPTNHLDIQSRNVLIEALQSYDGTFIVVSHDRHFID
                     TICNKVWYIENGKVYEYPGNYSEFQYHIDHKSPANEHNQKSSGSDEQIDTEVYKSDKS
                     KKRLDAEKRNNAFKELKEKGIINFNSWDLLTENQLKNVIKELETEIEQKESRKKELEK
                     LLEKEETFKDLEYSNKITIELGNLDKEIEISYNKWSDISKSQEI"
     gene            15476..>15955
                     /locus_tag="AC159U_00355"
     CDS             15476..>15955
                     /locus_tag="AC159U_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3335540.1"
                     /translation="MLSILQYCKDLQTVTKNEGEILLSQGSKSDKFYIIKHGECEILR
                     SDFQINKISEPGSMLGEMSILLDNPHMATVRCTKDSEFYVVDNASDFLHSNKDITYHL
                     AKTLAQRLNGVTSYLVDLKNQFEGRDDHFSMVDEVLQALVSDQDDYQIELGSERSKDA
                     "
BASE COUNT         4752 a         3012 c         2802 g         5389 t
ORIGIN      
        1 taatatatac ccctggtttt ggtagcagtt cccagtctgt ttttagattt gctgtgggaa
       61 aaccaagtaa tctgcctctc ttttcacctt ctgttactgt gccttcgaga ctaaagtttc
      121 taccaagata tttgttggct ttacttatgt gaccttcgag aataagattt cttatagctg
      181 tgctgcttat aacattactt ccatttgtaa caggatcaat aatagtggct ttaaacccca
      241 attcatttcc cattttttta agcagctcat aattgccgcc ccttttgctt ccaaagacaa
      301 aatcatggcc aatgaatatt tcctttagat ttaaaacatt tacgagtgta tctctaataa
      361 attgttctgc tgtaattttt gaaaattcct tatcaaacgt aaaacagacg gctatgtcta
      421 tatcttcttt ttcgaaaagt tttactcttt tttcaaatgg agtgatgaaa ggaagttttt
      481 gcccggttaa aacttcctgg gggtgaggat caaatgttat tacacacgaa gaaagtccct
      541 ttgcacgagc ctctttttta acctggtcta taattgattt atgcccaaga tgaactccat
      601 cgaatatgcc aatagtagca cagacagatt tacctatatt ttctttagga tctattaaaa
      661 gctgcatatc taatttttcc aaaatgaggg agtaaatata acaaggatag caaaaagttc
      721 tagtctgccg attatcataa gaaatgaaag cacggtcttg gacaaattgg aaagacctgc
      781 gtaattactt gcagggccta caagatccat gcccggtccc acattaccta tagtagcagc
      841 gcaagctgta aatgccgtaa tgatcggaac ttcatccaga gttagtatta tagtggataa
      901 gataaagata aaaatataga tcaagaagaa atttattatg ctggatacta cattgtcatc
      961 cagcacattt ttaccaaatc ttattggaag gacggcattt ggatatatat tcttcttaat
     1021 ctctctgaat gcatgtttta taagaattat aattctcatt acttttatag agcccgatgt
     1081 agaaccgctg cacccgccca caaacatcaa gattattagc aaccatttag aaaactcagg
     1141 ccacaggttg tagtcagtgc tcacaaatcc cgttgtggtt gaaattgaag taacgttaaa
     1201 tgcagcgtat ctgaatgatt cgaatagatt agagaactga tatgtgtaca gatcaattgt
     1261 taccagggtg atgcttacaa caatgatgat aacgaaaaac ctgaattccg ggtttgaata
     1321 cagcttttta aatttacctc tggaaagcca aaaaagtagt gcaaagttta tccccgaaat
     1381 aaacatgaat gctgttatag taaactcaac agcaacgctg ttatacgccc ccacgcttaa
     1441 gttttgattg gaaaaaccac ctattgagac tgtactgaat acatggatta tagaatcaaa
     1501 aatcggaagt ccaagtagat ttaaaatcac aaataacaga atagataaaa cgagataaac
     1561 cgtccataga tgcttagcgg tttcagcaat acgcggggtt agcttttctg ctgatggtcc
     1621 cggggcttct atgtttaaaa gctgcatacc acccactgaa agctttggca ggatggcaat
     1681 agctaaaatt aaaattccca ttccacccag ccactgagta aagcttctcc aaaacagtat
     1741 gccgtgggga agtatctcta tgtttactac agtgcttgct ccagtagtag taaagcctgc
     1801 tgttgattca aaaaatgcat ccattggatt tgagaaaact ccgtatatta gatacggcat
     1861 agagccaaaa gcaattgcgc ttatccagca gaagcttgct ataaagaatc cgtcttttct
     1921 attgaactca tcaatatcgg cttctgattt tgtaagctgt tcacctgaaa tacctactaa
     1981 gaatgtaatt actccggaaa tcaagaatga atatatatca tcttctttat aaataatgga
     2041 acagaaggcc ggaatgagca ttaatagccc aaggagtttt aaaaacctgc aaagaatata
     2101 tatagagtgt tttaggttca ctatgataag attttttcta ccgtttttac ggcacctgga
     2161 agacaaaaga tgataagcat gtcacctatc tcggaaacat agccacctct tggaattatt
     2221 atttcctctc cacgcactac gattcccaca atagagtctt tgggaaatcc agccttgtgt
     2281 agtggtactc caagtatttt agaatgttcg tgtatcataa attcaagtat ttctgccttt
     2341 ccttcttcta taagtgctac atgtgccaca cccttgaggt gaagcatgct taaaatttca
     2401 tttgctgtag agagccttac gcttatagcc ctgtctacac ctatggattc aattagatct
     2461 atatagtctg gttttgtata taataccgtt gatctctttg tgccaaggtt ttttgcaagc
     2521 agagcgctta gtacattttt ctcatcgtct ccggtaagtg ctacatagta atcagcatga
     2581 gcggcaccgg cttccttaag aatctcttta tttgttgcat ccccatcaaa gaccattacc
     2641 tcgggcagtt cttctgctat aagttctgct ctttcgtgat ctgattctat tactcttact
     2701 tcaatcccat tttctattaa ggtccttgca actctccttc ctactctgcc accgccggat
     2761 attatagcca tatctacttt ttctctcttt acccctaaaa gagcttcgag cttatccgaa
     2821 atacttggaa tccccagtgc aaatacataa tccccttttt taagtctcgt gtcccccgtt
     2881 ggaattgaaa tctcaccatc tcttgatata gctacaaata tccaagcctg aggcgggtcc
     2941 atttcagaaa gctttttgcc ttcgataaga gaattatctt caatcctaag cttaagcatc
     3001 ctgattttcc ctgatgcaaa tgtttctgtt ttccaggcaa aaggtgtgct tatgaggttt
     3061 gctatttttt ctgcaaccag caaccctgag ttaacaactg taatttgagg gatttgtata
     3121 acctgaggat attcaaaata atcagggtct ctaatccgtg caattattct ctctattcct
     3181 aaagaatgtg aaattattga tgctaaaata tttgttctgt cgtttcctga tacagcaagc
     3241 attatatctg ctttttctat ctctgcctgt ttaagcacat tggggttatc tccctcacca
     3301 tgtattataa gggcatccag gtgttctttt gcatattctg tctgttgttt atttttttca
     3361 attataacaa tatcgtgttc atcggagaga ttatttgcta agaaagttcc tacttcaccc
     3421 gatcctataa taactatacg cataagttaa gctctttttt atgttattgc aggtttattt
     3481 agcataaatt ttaatcttag aaggtttagt actccaaata taaatattac aatagatatg
     3541 agctgagctt gcgagaggcc atcgatgaag cttggagagg tgtttctgat aaactctata
     3601 aagaatcttt gtattcctaa aatgataagt gttaaagaaa acatccagcc tgttgggaaa
     3661 tcggactttc tgattgccca gagaattaaa aatatgaata tcattaaaat cgtatcatag
     3721 atttgagtag ggtgaactaa ctgagtagta gggggtgagc cgtttggaaa agacagcgcc
     3781 cacggcacag atgtgggggt accataatca tcgcccacaa gcaggcatcc gatccttcca
     3841 atgccataag ctaaaacaag tcccggggcc gtactgtctg ttagagtcca aaaactaatt
     3901 tttttccata ttcctacaac aaatataaga atagaagctc ctatcaaacc tccaagaaaa
     3961 gtaagacctg aggataaaaa tttgaggggt tcgttcataa actcagcaaa tgtagcgttt
     4021 tggaataaaa acagtatctt tgctcctccc agtcctccga ataacgcagc aagtaatacc
     4081 cagtcggcaa ttgaatcatc aagccctctt ctctttattt ccagacttaa gaatatgtag
     4141 gctgcaaggt aggccagggt taccataaca gaatatgatg atatcgtgat acttcctatt
     4201 tggaaaagag ttgggtacat gtttgttctt ataaattatt tggttgattt ttagttaaaa
     4261 agatgaccag aataattaaa aattcaataa taattttaaa ttcgggtatt taataaaaat
     4321 tatttctggt tgtggattga aaattatgat tcgtgtttac ttgcatcaac gattctgagt
     4381 cttatattat tacttccatt ccatctgttt atttccggtg tatagactat atcaatttta
     4441 tttgccggtt caagctgcag tttgcaatta aaccatacag catcatagga tttcgtagtg
     4501 tttttcagtt tgaggctcag atgtttttct ttaaaaatct ttctgtccac aacttctagg
     4561 gacttagtaa aaaataatgg ttccggattt cctatgccat gaggtgctag gttttcaaat
     4621 tccattatta gttctttgct tagatcatga gtctctattt gggcatctac tattattttt
     4681 tttacatatt tatctttaaa acctttcagt gctttatcaa actgaagttt aaattgatcg
     4741 atgttttgct cttttatagc tatgccggcg gctagatcat gtccaccaaa ttcttcaaag
     4801 cagcctgcac attttgtaag tgctgtatat atatttactc cctcaagact tctgccggag
     4861 cctttgccca ttccagtatc atcaatagcg ataagaaagg tgggttttaa atatttatct
     4921 acaattctgg atgctacaat tcctataacc ccctgatgcc agtttcttga tgccagaact
     4981 atagaatttg aatctttgat tttgggatct gagtctatca tcgaaaatgc ttcttttagt
     5041 atttttgctt caataccctg tctattggtg ttttcagtgt tgagcagtcc agcaagctct
     5101 tttgctgtct gcggattatt tgagataaat aattcaactg cctcttttgc agtatttaat
     5161 cttcccgatg catttattct gggtccaagc ctgtagccta tgtcaaaaga gcttacttcg
     5221 cccgatatgc tgctcgcttc ttttagtgca ataagtcctt gccttttcga agaacccatt
     5281 cttttaagac cctctttaac taatatcctg tttacatttg taatagaacc acagtcggca
     5341 acagtaccaa gggcaacaag atcaaggtaa gctcccaggt ttggttcctt accctcaaaa
     5401 aaacctgatt ctctaagctc tcttctaagt gcaagcacaa gattaaatat aacccccaca
     5461 ccggtaattt cttttgccgg gtagttgcat tgtccaagct gagggtttag tactgcaaca
     5521 gcatcaggga tttgatcagg gggtttatga tgatctgtta caataaaatc aataccaagc
     5581 tctcttgctt tttccacttc ctgcactgcc gtgatcccgc agtcccctga aatgatcaat
     5641 tttacgccct gtttatccag tttttttata gcttcgatgt ttaccccata cccttcggta
     5701 aatctgtccg ggttataata ggtgactctg ctgccaatct cttttagaaa attatataag
     5761 agtgctgtgg atgtaactcc atcaacatca tagtcaccgt atatacctat aatttccatg
     5821 ttctctattg cctggattat tctctttact gctttttcca ttccgtgcat aagaaacggc
     5881 gatggcaggt caaagaggct tgagtttaaa aactggtggg cttcttctgc ggtattaata
     5941 cccctgttaa ttagcagctg ggcagtgata ggggaaattt ccaatgtttt ggagattttc
     6001 tcacttaact tctgattttc tttttgtatg atcaaccttg ttttcattat ggttggatta
     6061 ttttttagat tttaaatgat ttgttatagc ttttaatccc agaatatagc tataagttcc
     6121 aaagccggat attacgccca aggatacccc tgatacatat gagttttgtc taaatttctc
     6181 tcttttataa atatttgata tgtgaacttc aaccgttggt atgtcggttg aaagaatagc
     6241 gtctcttatt gctacacttg tgtgcgtgta agctgcagga ttaatgacta ttccatcaat
     6301 attcccaaga gaattttgta tctttgtaac aatttcactt tcactgttcg attgaaaata
     6361 ttctgtctta acatttagtg atttagctac tatttctatc agattgttaa tatctgatag
     6421 tgttgtgctt ccatatatat cgggttctcg tttgccgagc atattcaggt ttgggccgtg
     6481 tataattagt atattcattt tagtcaagtt cctctgatgc atcaaaactt tttttactaa
     6541 tggattttga tgccatagat aaagaaaatt tagttttaag atggggatca tgcggatatt
     6601 tttttacaag ctgctctaat atttctgcag ctttttcgta gtatccctga tttatataca
     6661 gatctgccat ggtctctgtt attaagttta cctcacgtgt tgtatgtttt acattttgag
     6721 atgaatcact gagcttgttt ttaatattct ttgtgatatc gaaatgagaa ctttgagtta
     6781 aactcttatt ttttagtctc tctatttcat aaagggtctg tgatttatta gcacaaaaaa
     6841 ttagtttttt atataaaatg tccagagcct ttgatgagtc gttttcgctg ttatatattt
     6901 tgcttaacat atcaagggct ttttcattgt tcggtgtttg atctattaat tgttccagat
     6961 acttttttgc ttcatctatc atccagcgtt cataatatat ctcagcaatc atgagttttg
     7021 ctgaaatgtc attttgattt tttctaagag ctgcaaacag aattgacagg gctttatcta
     7081 tatccccctg tgatttgctt atcccagcca gtataagatc gctattaggg tcttgtgcat
     7141 atcgttctat taaagattct agaagattgt ttagagaatc ggtttctgta ttcatacaga
     7201 gagttataaa atttattcag tatactactt atagattaaa actgttttcg ggctcttgca
     7261 agtaaatcgt ccaccggttc attatttcta tctatccagc ctttctcagt tttaggctta
     7321 attcgaagtg ggtcagtaaa aagtctgact aaaattgcaa agggcataaa catcgtgaaa
     7381 taaaaaatgg tgagtataag ccttgcctga aagttgccaa tcctgtgtgc acgaggcacc
     7441 caccacttcc agaggcgttt gtgaacaggc agttttatta caacatcgtc taaaatttct
     7501 tccatatttg tagtaaaaat taataaaaca ctaattatac aataggtatt atgtaagtaa
     7561 atttaataat catcacagaa tatttctaag ttcttccaaa aggaaaatac tcaaatccca
     7621 cctcttttag ttttgaagga ctatatagat tcctgccgtc aaagatcaca ggtgaattca
     7681 taatttcttt aagtttatta aaatcaggct gtctgtactg attccactct gtatgtatta
     7741 caagcgcatc ggcaccttca caggcatcat agttatgctc tacaaattct atagagcctc
     7801 caagctcttt ttctgcgttt tctgtggcta ccgggtcgtg ggcagaaatt ttggctccat
     7861 ttttaagcag tttttctatt atataaagtg caggggcttc tcttatgtca tcggtgttgg
     7921 gtttaaatga tattccccat accgctattt ttttgttttt taaattgccg ttaaaatagt
     7981 tgttaatctt ttgccagaaa atttcacgct gatactgatt ggcttcatcc acggcgctgc
     8041 atacttttaa tttataccca ctttcttctg cggtttgtat aagagctttt acatcctttg
     8101 gaaagcagga gcctccatat ccaactcccg gaaaaagaaa agcagtacct attcgtttgt
     8161 cagagcccat tgcctttctt acgtccgaaa catcagcccc tattctctca cacagatttg
     8221 ctacttcgtt cataaaagaa atccttgttg caagcattgc gtttgatgca tattttgcaa
     8281 gctccgatga tctaatgctt acgatctgcg ttctgtcaga tgatctcata aacggctcgt
     8341 aaagctgctt aagcttttca ccaacttgtt cattgtcgac tccaatcaca actctgtctg
     8401 gtttcatgaa gtcttcaact gctgcacctt cttttaaaaa ctcagggttt gaagcaacat
     8461 caaaatcaat atctgtttgt tttttcacta tgtctcttat catttcgctt gttccaacag
     8521 gaactgtact tttggttaca attattttgt agtccgtcat gtttttgccg atagactcgg
     8581 ctacgtttct tacaaaggaa agatcggcag agccgtcttc ttttggtgga gttccaactg
     8641 caataaatac aatttctgat ttatcaagtg cagacttaag atcggtcgta aatgtaagtc
     8701 tgccttcgtc aaagttttcc tttaccagtt cttctaaacc cggttcaaaa aaagtaacct
     8761 taccttttga caggcttttt actttttctt catcaatatc aacacatgca acattattac
     8821 cgcttcctgc aaagcatgcc cctgttacaa ggcccacata tccagttccg atcatacaaa
     8881 tattcattat aaagctttcc tccctaacta atccaaaaga tactgaagcg ctaaagttaa
     8941 cccatactat aaggttattc aaccccaatc tatgtgtttt ttattttttt atcatactgg
     9001 taatcttatc aaactcttca attttaagta tttttgataa tccactatag ataacaatac
     9061 ccgcaataag acaaacgccc agtgctccaa ctttattaat atcaaagtta ctcccggtcc
     9121 agtctgtaaa cagtgataat tgatacacag atattcccat aattgtccca atcagaatta
     9181 ttttagacat atatcccacc agacttgcga tttcaatatt tacgattttt ttatttatta
     9241 agtatataag aagtgaaata tttagcaccg atgatgctga gcttgaaaga gcaaggccaa
     9301 tatgatttag ctcaagtttg aaacttaaaa catatcccag tacagcattt aaaataaagg
     9361 cagtgaatgc gatatacact ggagttttgg tatctttttg agcaaaaaat gcgggcactg
     9421 taatccggat aagacccaca gcccacatac ccagtgcgta gccaaaaaga cactgagagg
     9481 taaaaaccat ctgctcataa ccaaactctc ccctttgata aagtatattg cagatgggtt
     9541 tactaagcgc aataagccct atgcatgcag gtaccagaat ataaaaaagt attcggattg
     9601 agctcaaata tgtttctctg aatttattta ggtctccctt ggcgatataa gaggaaaaat
     9661 ttggtaaaag tacggttgca agagatacgg caataatacc aagtggaaat tcaattaatc
     9721 tttcagaaag aaatagataa gatacggttc cttcgggaag gtaagaagca tactgtgtat
     9781 taactatgat gttcaggtta tatactgcta gtccaaacac ctgagggata ataagggaga
     9841 atatttttct tacatcggga ttattaagga caagtttagg gttgaacaag aagtttctgc
     9901 tctttaaaaa aggaatctgg aaaaacagtt gtaacaaccc tcctgcgatt acacctatag
     9961 ccaaggtaat tatggggata tcaaattggt catgaagcaa cagggcaaag agaataatgc
    10021 agatattaaa tagaactggt gaaaatgcag gtgcaaagaa gtgctttaaa gagtttagaa
    10081 ttcccattga tagagctgta atagatataa agagaatata aggaaacatt attctgttta
    10141 gctttacagc cagatcaaat acatcatcag aaaatccaga tgcaaatatt tttactatat
    10201 agggggaaaa gatgattcca agaagcgata taatcacaag aagacaaaca agtatagtaa
    10261 atatggaatc agaaattact tttgcttttt ctttaccacc gtattcgagt ctttcagtaa
    10321 acacgggtat gaatgaaaca gttagtgtgc cttcagcaaa aagtcttctt agaaggtttg
    10381 gtattctaaa tgcaacccaa aatgcatcgg tcacctgacc agcgccaaat agtgaagtta
    10441 taacaatatc acgaacaagc ccaagaattc tactgatgaa tgtaaaaaag cttacagagc
    10501 ttgctgattt tgctatagtc tttttatcag tcattttgta aaccgtattt gtccaggtgt
    10561 tttacttcca atacctcaaa ttctatttct tgttttaggt tatccaggac ctgcatggtt
    10621 tcttccaaca tatcatcagt tggtgtaaca ctaataatat cctgatcggg gatttcagtt
    10681 ctaacccagg cgaggtgaga atatgttccc agcagactgt ggaatatcac agtatcactt
    10741 ttatttttaa ttttaatcag tatttgaggc ataagttata tttttaacct tgaaacggca
    10801 aatttaagat atacattata tattaatatt cagtaatttt atttatatat tatttagggg
    10861 gtttaatgaa tactttaaaa acattaattc tacttgcttc attatctgca ttaataatct
    10921 ggatgggtgg cgctattggt ggcagcacgg gtgctactat tgcagtagta atagctctag
    10981 caatgaattt tgcaagctac tggtggagcg ataagattgt gctatctatg tataaagcca
    11041 aggaagttac tcctgaacag gctcctgagc tttataatgc gactcttgag cttgctcaaa
    11101 atgcagggct tccaatgcca aagctctata tcattccaga agatactcca aatgcatttg
    11161 caaccggtag aaaccataat cattccgcag ttgccgttac atcagggatt atgagaattt
    11221 taaacatgga tgaactaaaa ggagtaatag cacatgagct ggctcatatt aaacacaggg
    11281 atattctgat aagctctata gctgctacta tatccacggc tattacttac ctgtcttata
    11341 tggcaatgtt ttttggcggt ggaagagatg ataacagggg tggaaatccc atagcgctta
    11401 ttgctatgat gatacttgct cctttggcag caactatcat caggatggcg ctttcaagaa
    11461 caagagaatt tctagcggat aaaggcggtg cggagatttg cggcaatccg ctacaccttg
    11521 caggtgcact aagaaaactt ggggagtatt caggaagatc gcagcttcag gtaagcgagc
    11581 aggtagcaga cagcacggca catatgttta ttgttaaccc tcttctggga aagggcctgg
    11641 cagcgctttt tagtacacat cctcctatgg agcagagaat cgaaaaatta gaagacatgg
    11701 cggcaggaat agtgagataa taaattatga atgaaattga taaaacaata gaaggtaaat
    11761 tttatatagg ccaggtaata cagcataagc tctttgatta caggggagtt attgttgatg
    11821 ttgactctaa atttgaagga tcagaagaat ggtatgaagc agtagcacag tcaagacctc
    11881 caaaagagaa accctggtat catattctgc cagatgggtc tgcacataag acttatgttg
    11941 ctgaaaggaa tatagaaaat gataaatctg gtaagccaat agaacaccca gccgtaggag
    12001 cattcttctc aggatttaaa gacggtacct atattacaaa taaagggttt aactagtaag
    12061 ctaaggatta atatatcctt ttgttaattg cggaaattaa acctgaatgt gtaattaaaa
    12121 tttaatgtgt catttttccc gacattaatc tgaattacat taccccccct aatgcgtgaa
    12181 taaccaccct gtccggcaga caggatgcgg ttaaaagggg tgttcatgtc tataagttct
    12241 tttccgttac tgcttaatat ttttttaaga gaattgcctt cagtaagatc ataggtatgt
    12301 gtactatcca cataaggtcc agtttttgtc tgtcttcctt tatttaaata ccaaccttta
    12361 ttcgtccatt ttccaatcaa aagattttca aactcggggt cgccatttgt ttttactccc
    12421 ttgtcataac caaagttttc aaaagttccg gctttgtata tgtaaccttc gactataaaa
    12481 gaagttttcg tataattatt ttctgaatca gtttgagaat ttaaaaattt aaatgtatga
    12541 ccattacagc atacatccag ctccatatcg tattcttgtt gatcactttc agaatttctt
    12601 gatcttctgg aggttgtggc actagttctc acattatcca gagtgtccag gctcttcctt
    12661 gcaattcttt ctataaatgt cataagatca ccccaaatta ttagacataa actaaaacag
    12721 caattgccta aaaggatatg tgaaggaagg ttttagaata aactactcac ttcggcaacc
    12781 tgactaccat aggttattaa tattaatctc tactaataac cggtaggctc aaggctttgc
    12841 gtcccgtcct ttcaaacggt ttgccatttc ggtttaggac tatttattct taagtttgat
    12901 tctagtacaa tcccaaagta ttgtcaatcg ggaaaaataa aatcagacta tggtactaca
    12961 tgggtttata caccgcgaaa tacactgcaa ttattgctaa aatcggtact gtaaaccaga
    13021 ggatagctgc ttgtggctgc agcttcttta taaaaaatgt catgcctccc attattaccc
    13081 aaataacaag ttttccccaa acccaagggg gccagtccat gccatgaatt attcccagtc
    13141 ttgccagaag accaaatcct ccaagtaaaa ctatgaacat ggaaattcca tgcatgatac
    13201 ccgcaagttt tttaaatttc tctatattgg agttaattac tatcagatat gtcgacagtg
    13261 agaataaaag aaaaaatacg gctgctacat gaagcacttt ataagtttca tatgaaatca
    13321 tttcaatctc ctattattta tatttcctgc gattttgaaa tatctgacca cttattatat
    13381 gaaatctcaa tctctttatc caggtttcca agttctattg taattttatt cgaatactcc
    13441 aggtctttaa atgtttcttc tttttctaaa agtttttcaa gttccttctt gcggctttct
    13501 ttttgttcta tttctgtttc taactcttta attacgtttt ttagctgatt ttctgtaagc
    13561 aggtcccagc tgttaaagtt tattatgcct ttttctttta gttctttaaa tgcattattt
    13621 cttttttctg cgtccagtct tttttttgat ttatctgatt tatagacttc agtatctatc
    13681 tgctcatctg atcccgaact tttctgatta tgttcatttg ccggactttt atggtcaata
    13741 tgatattgga actctgaata gttgcccggg tattcgtata ccttgccatt ttctatgtac
    13801 caaaccttat tacatattgt atctataaag tgacgatcgt gactaactac aataaaagtg
    13861 ccgtcatagc tttgtagtgc ttctataaga acatttcttg actgtatatc aaggtggttt
    13921 gtaggctcat ctaaaattaa gaagttttta ggactaagca gggtctttgc aagggcaact
    13981 ctgctttttt ctccgccgga aagaaccttg atctttttaa atacatcgtc accagagaac
    14041 aagaaattac ccagtattgt tctaatatca gtttcattac ttacctttga atctgaagaa
    14101 tgagcaagtt cttcaaatat attgtattcg ggcttaagag tctttgcctg attttgggca
    14161 aagtaagtta gctctacttc gtaacctgtt tttctctgcc catcaaactt ttcggtaccg
    14221 ttaattattc taactagggt acttttacca attccgtttt taccaataag agcaatttta
    14281 tcccctctta gaattgtgag aggatcacaa tctttaaaca cttcattgct gccataagat
    14341 tttgaaaatg cagatatctc taaaacattt ttccctgagc gcttggtatt gttaaatcta
    14401 aaatttacag atttgtctgt gcctgtttct tcgattcttt caagcttatc aagcatcttt
    14461 attctgcttt gcacctgctt tgccttggtg tttttatatc tgaacctttc aatgaacctc
    14521 tcatactgtt ttatctgtct ttgctgattt tcatatgctg acttttgtat ttccacattt
    14581 ctcattctga gttcaagata tttactatag ttgccctgat actccgtgat ttttccgcct
    14641 gagagctctg ctgtgatttc agtcattctg tccaggaaat atttgtcatg agagactaca
    14701 atcacactcc cttcaaatga tttaaggtag tcttcgagcc atttgatact ctcaatatca
    14761 aggtgatttg taggctcatc caaaaataag aattcaggat tttgtaaaag aagctttgca
    14821 agctccactc tcattctcca accaccagaa aatgtagaaa gagggctgct taaatcatta
    14881 actgtgaagc caagccccat aagcatcttc tctgctcttt tatttacggt ttcagaattt
    14941 tggatcttta actcattttg tatagaatcg atttttttaa gaagcagttt gtattctgga
    15001 gttgtttcgt ctttatattt agaaagatca tgtaatagtt gctcattatc ctgctcaagc
    15061 tgtttaatat ctttaaatac agtgagagtc tcttcaagta gtgaggtttg agcattatta
    15121 agagcaaatt gctgaggcag gtaaccaata accgaactag atgggagtga tacactgccc
    15181 tcatctgctg ttagggagcc cgtgataagt tctaaaagtg ttgttttgcc tgcaccattg
    15241 ggccctacaa gaccaatttt cttgcagtct tttatgtgcc agtttagccc gtttaaaatt
    15301 ttataggagc caaaagaaag gcttaagttt tttattgcaa tcatttttta gtaaataata
    15361 taattaaaat gttcacaggt taaaatataa gtttaaataa tacatttgtt agaatttatg
    15421 tttataaaat taataaattg tgtagtatct gcaaaaggaa aataaaaaag taaatatgtt
    15481 atcaatacta caatactgta aagatttaca aacagttaca aagaatgagg gagagatatt
    15541 actttcccaa ggcagcaaat cagataagtt ttatataatt aaacacggag agtgtgaaat
    15601 tttacggagt gattttcaga ttaacaaaat atctgaaccc ggatcaatgc tcggggaaat
    15661 gtctatacta ttggataatc cacacatggc tacagtcagg tgcacaaaag attcagaatt
    15721 ttatgtagta gataatgcat cggatttttt acactcaaat aaggatatca catatcatct
    15781 tgcgaaaact ctagcacaaa gattgaatgg ggtaacgtcc tatttagtag atctaaaaaa
    15841 tcagtttgag ggaagggatg accatttttc aatggttgat gaggtgttgc aggcgctggt
    15901 ttctgatcag gacgattacc agatagaact tggttcagaa aggagtaaag atgct
//
LOCUS       DBNGSV010000004        10537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_139209, whole genome shotgun sequence.
ACCESSION   DBNGSV010000004 DBNGSV010000000
VERSION     DBNGSV010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_139209"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2974)
                     /gene="rpoC"
                     /locus_tag="AC159U_00360"
     CDS             complement(<1..2974)
                     /gene="rpoC"
                     /locus_tag="AC159U_00360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3335541.1"
                     /translation="MSMDIDTLFEKPKNPSDYSSVKISIASPDKIKSWSFGEVKKPET
                     INYRTFKPERTGLFCAKIFGPVKDYECLCGKYKRLKHRGHTCEKCGVEVITTKVRRER
                     MGHIELASPVAHIWFLRSIPSRIGMLLDMTLKDLEKVLYYESYVVTDPGQTPLKFAET
                     VSEERYRRALEEYGMSFKVGMGAQTVRDMLKSIDLVELSEILRVELRETSSPIKRARI
                     SKRLRIVEAFRNSKNKPEWMILTRIPILPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLRKLLELGAPDIIVRNEKRMLQEAVDALFENGRRGRPVLGHNHRPLKSLSDIIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPDLKLHQCGLPKQMALELFRPFIYQRLEKWGAA
                     ATIKIAKKLVEQEAPIVWDALDEVIQEHPVLLNRAPTLHRLSIQAFEPILIEDKAIKI
                     HPLVCPAYNADFDGDQMAVHLPLSLEAQTECRTLVMSTNNILSPAHGKPIILPTQDIV
                     LGVYYMTRDIPYEKGEGKTFYSIDDVRFAYDANEVAIHSKIKFHLNENFYDTTVGRVL
                     LYEILPEGLDFELINKVMTKKAIEVLVDTCFRTSGGKNTVLLADRLRTMGFQYSTRAG
                     ISISIDDMIIPERKKDLLDKANSEVVKVQNQYSQGLITDGERYNKVIDIWAKTGDEVA
                     HEMMDELSTDTIRTNEGEEVTRPTSNPIFMMVESGARGSQAQVRQLAGMRGLMAKPSG
                     EIIETPITSNFREGLTVLQYFISTHGARKGLADTALKTANAGYLTRRLIDVAQEVMVN
                     EHDCGTIEGITVSDFVEGGQVIDRVGERVLGRVALEDIIDPTTGEVIVKENNEIDEEA
                     AHRIDEAGNLSEVKIRSVLTCEARHGVCILCYGRNLSSGKLSNIGEAVGIVAAQSIGE
                     PGTQLTMRTFHIGGAASQRVAESTLDSQHEGTIKLVDVKSVMNKEGHLIVTNRTGVLA
                     VIDDKGFEREKYPLVLGAR"
     gene            complement(3006..7136)
                     /gene="rpoB"
                     /locus_tag="AC159U_00365"
     CDS             complement(3006..7136)
                     /gene="rpoB"
                     /locus_tag="AC159U_00365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3335542.1"
                     /translation="MSHNLNGANTLRFRKSFSKIPPILENPHLLDVQIKSYLEFLQID
                     VDPEQIQNQGIQRAFELVFPVEDYNEKATLEYVKYRFEKPKHEPLECRLKGYTYVAPL
                     YVTFRLVIWESPEDAPEEKSIRDVKEQEVYFGEIPLMTENGAFIVNGTERVIVNQLHR
                     SPGVFFDQVSAKDTTTGRSTYSARIVPQDGRWLDFEFDAKDLLQVRIDRKKRFPSSVL
                     LKALGLSNREIMYYYYPSETVYMDGDDVHKSIDPELLSYQRCSVDIVDPNTSKVLVKK
                     GRRFVKNSIDRVTSAGLDRIPILSTDIIGRISADELKDANDEIILNFNEEIDEEKLEV
                     LKSSGVKEFKLFYIDNVSVVASLRNTILVDKIANTEEAIIDIYKKFRPTDPPTYNVAE
                     TFFNNMFFNPDTYRLSQVGRLKINYKLDHGISEETMTLTKEDILESVRYILNLRGGRG
                     STDDIDHLGNRRVRTVGELVEDRFYHGLERLARSVKEKMGYQAVENLMPSELVIPKTV
                     ISVVKEFFTTGQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHSSHYG
                     RICPIETPEGPNIGLISSLSTYAKINDHGFIQTPYRIVENAVVTKEIRYLNALEEEQY
                     IIAQANAPIDENGKFKNDLISSRHKGEFLITEAENVQLMDVSPAQLVSVSASLIPFLE
                     HDDANRALMGSNMQRQAVPLIKSKAPLVGTGFENIVAKDSGVTVVAKRDGIVEFVDAS
                     KIVIRGIEEENGAEAGVELYNLLKFQKSNQNTCWNQIPIVKKGEKISGGQIIADGPST
                     QYGELALGQNAIVAFMPWGGYNFEDAILISQRLVKEDSFTSIHVEEFECIARETKLGR
                     EEITRDIPNVGDDALRNLDESGIIRIGAEVRAGDIIVGKISPKGETQLSPEERLLRAI
                     FGDKAGDVKDTSLKASPGVNGTVIDVKMLVSRVAEKDERAKSIEDFEVTKLKQDRDDE
                     IKILKEDSVDKIKTKLSGKTSASKLNVNRKAVLKPGDTITDEILDTIPFEKLADISVK
                     ESESIEHEVKKVIERTLEQTDLIKMVYDNKLTKISKPDELPPGVLKVVKVYVAVKRKL
                     SVGDKMAGRHGNKGVVSNIVPVENMPYLPDGTPVDMVLNPLGVPSRMNVGQILETHLG
                     WAAKELGNQIQEYMQTEYSPEVLKKKLMDIFDEPNVNKYIKQLNDEQVLELIEKYDEG
                     IPVKTPVFDGTEESEIKSMLAQAGLPLEGKTILFDGRTGEPFDQKVAVGVMYMLKLHH
                     LVDEKIHARAIGPYSLVTQQPLGGKAHFGGQRLGEMEVWALEAYGASYTLQEFLTVKS
                     DDVIGRTRVFDSIVKGNMDFEPGIPESFKVLQNELQALALDIELQEEEEETTTSAL"
     gene            complement(7221..7610)
                     /gene="rplL"
                     /locus_tag="AC159U_00370"
     CDS             complement(7221..7610)
                     /gene="rplL"
                     /locus_tag="AC159U_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3335543.1"
                     /translation="MAEVTRADVLTYLENSTMLELSELIKEVEDKFGVTAAAPAVAVA
                     APVGGADDQAAAGAEKTEFNVVLQSIGDNKIQVIKAVREITALGLKEAKELVESAPKA
                     IKEGLSKEEADDIKKKLEETGAGVEVQ"
     gene            complement(7664..8185)
                     /gene="rplJ"
                     /locus_tag="AC159U_00375"
     CDS             complement(7664..8185)
                     /gene="rplJ"
                     /locus_tag="AC159U_00375"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3335544.1"
                     /translation="MLSKQQKIDLAKDYGEKFKEASSVFVLDYKGLTVKEIQDLRRSV
                     KKANAQFSVVKNSVLKYGAEGSEVEKIADMFIGPTAVAISKEDPVSIAKAFVDSIKTL
                     PQVKLKGGLVDGSVLSESEITSLSKLPSREAMLGQFVGMLNSPLTGFLATIKNMQTKL
                     VYALNEVKNTKES"
     gene            complement(8208..8930)
                     /gene="rplA"
                     /locus_tag="AC159U_00380"
     CDS             complement(8208..8930)
                     /gene="rplA"
                     /locus_tag="AC159U_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3335545.1"
                     /translation="MASGKNYIAAKQKVDIDKLYSVDESLGLISETKVAKFDETVELA
                     IKLGIDPRQANQQLRGAKVLPHGVGKDIKVLVFAKGEKVKEAQDAGADYVGSDDLVEK
                     IQNENWFDFDVAVATPDVMSSVGKLGKVLGPRGLMPSPKVGTVTFEVKKAVEDSKAGR
                     IEIKNDKGGVVHAPFGKSSFSVDKLKANFLSLLDAIMKMKPSASKGVYLRKIVISNTM
                     GPGIKVDINDIKEQIKTFDFAA"
     gene            complement(8952..9368)
                     /gene="rplK"
                     /locus_tag="AC159U_00385"
     CDS             complement(8952..9368)
                     /gene="rplK"
                     /locus_tag="AC159U_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3335546.1"
                     /translation="MKKEVGQIKLLIEGGKASPSPPVGPALGQRGVNIMEFCKAFNAQ
                     TQNSPDLLPVTVIVYADRSFSFVVKTPPVSYLLKKAAKIKKGSSEVPRVKAGKVTKDQ
                     IQEIANTKMEDLNADTIESAMRIIEGTARSMGLEIQ"
     gene            complement(9371..9928)
                     /gene="nusG"
                     /locus_tag="AC159U_00390"
     CDS             complement(9371..9928)
                     /gene="nusG"
                     /locus_tag="AC159U_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3335547.1"
                     /translation="MSKRWYVVHTNTGFEDRVKTYITDRAKAENLEDSISEVLIPTET
                     IVEAAKDGKRKTSIRKFFPGYILVNMELNEVTWHFLRDIPKVIGFVGGKLKDGKIDPN
                     SVPDIDENEVMKIKTQVEEGSLKPKPKIFYEKGETVKVNEGPFANFTGVIEEVNSDKA
                     KVQVNVTIFGRTTPIELDFNQVEKL"
     gene            complement(9952..10155)
                     /gene="secE"
                     /locus_tag="AC159U_00395"
     CDS             complement(9952..10155)
                     /gene="secE"
                     /locus_tag="AC159U_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR3335548.1"
                     /translation="MSNFNDLKNRTSDFLKDVEVEITKITWPFLNDTLKSTLAVVVIS
                     TILIGFLFGVDSLFSMVVKYVLA"
     gene            complement(10180..10253)
                     /locus_tag="AC159U_00400"
     tRNA            complement(10180..10253)
                     /locus_tag="AC159U_00400"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10217..10219),aa:Trp,seq:cca)
     gene            complement(10254..10412)
                     /gene="rpmG"
                     /locus_tag="AC159U_00405"
     CDS             complement(10254..10412)
                     /gene="rpmG"
                     /locus_tag="AC159U_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3335549.1"
                     /translation="MGDNTVNVTLACGDCKRRNYSTIKNKRNHSEKLEFKKYCRWCKK
                     HTQHKETK"
     gene            complement(10422..>10537)
                     /locus_tag="AC159U_00410"
     CDS             complement(10422..>10537)
                     /locus_tag="AC159U_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3335550.1"
                     /translation="NMVVELITEVAMEEKLRFAIREGGRTVGAGVVTKILE"
BASE COUNT         2837 a         2345 c         1672 g         3683 t
ORIGIN      
        1 gtctagcacc caatacaagt ggatattttt ctctttcaaa acccttatca tcaataactg
       61 ccaacacacc ggttctgttt gtaacaatca ggtgtccctc tttattcata actgatttta
      121 catcaaccag tttaattgtt ccttcgtgtt gagaatcaag agtactttct gctaccctct
      181 gacttgcagc accaccgata tggaacgttc tcatagtaag ctgggttccg ggctccccga
      241 ttgactgggc agctacaata ccaactgctt caccaatatt ggaaagtttt ccggatgaaa
      301 gatttcttcc gtaacacagg atacatacac cgtgtctggc ttcgcatgtt aatactgatc
      361 tgattttaac ctcagacagg tttcctgctt catcgattct gtgtgctgct tcttcatcaa
      421 tctcgttgtt ttctttaaca attacttctc cagtggttgg atcaataata tcttctagtg
      481 ccactctgcc aagcactctt tctccaaccc tgtctattac ctgaccacct tcaacaaaat
      541 cagagaccgt aatgccttct attgttccgc agtcatgttc atttaccata acttcctgag
      601 ctacgtctat gagccttctt gtaaggtaac ccgcattagc agttttaaga gcagtatcgg
      661 caagaccctt acgagcacca tgggtagaaa taaaatattg aagtaccgta agtccttctc
      721 taaaattcga tgtaatcggc gtttcaatga tctcaccaga aggttttgcc ataagtcccc
      781 tcattcctgc aagctgtcta acctgagcct gacttcctct tgcacctgat tcaaccatca
      841 taaatattgg attggatgta ggacgtgtta cttcttcgcc ttcatttgtt ctaattgtgt
      901 ctgttgacag ctcatccatc atctcatgag ctacttcatc tcctgttttg gcccaaatat
      961 caattacctt attatatctt tctccatctg taatgagacc ctgcgaatac tgattctgaa
     1021 cttttactac ttcgctattg gctttatcca aaagatcttt ttttctttca gggattatca
     1081 tatcatcaat agatattgaa attcctgcac gggttgaata ctgaaacccc attgttctca
     1141 gacggtctgc gagcagtacg gtgtttttac cacctgaagt cctgaagcag gtatccacca
     1201 atacttcaat tgccttttta gtcatcactt tatttataag ctcaaaatca agaccttccg
     1261 ggagtatttc ataaagcagc accctgccta cagttgtatc atagaaattt tcatttaaat
     1321 gaaatttgat ttttgaatgt atcgctacct catttgcatc atatgcaaac cttacgtcat
     1381 caatcgaata gaatgtttta ccttcacctt tttcataggg gatatccctt gtcatataat
     1441 aaacaccaag cacgatatcc tgtgtgggaa gaataattgg ttttccgtgt gcgggagaaa
     1501 gaatattatt tgtagacata actagtgttc tacactctgt ctgagcttcc agtgataaag
     1561 gcaaatgtac tgccatctgg tcaccatcaa agtcggcatt gtatgctgga cagaccagag
     1621 ggtgaatctt gatagcctta tcctcaataa ggataggctc gaatgcctga atactaagtc
     1681 tatgaagtgt aggagccctg tttagaagca caggatgttc ctgaattact tcatcgagtg
     1741 catcccatac aattggagct tcctgttcta caagtttctt ggctatttta attgttgcag
     1801 ctgcgcccca tttctcgagt ctttgataaa taaaagggcg gaacagttca agggccatct
     1861 gcttaggcag accacactga tgaagcttta aatcagggcc aactgtaatt accgatcttc
     1921 cagaatagtc taccctctta cctaaaagat tctgtctaaa cctaccttgt ttacctttaa
     1981 taatatcgct caaactttta agtggtctgt gattatgacc aagtacaggt cttcctcttc
     2041 tgccgttttc aaaaagtgca tctaccgctt cttgaagcat tctcttttcg tttctaacaa
     2101 tgatatcggg agctccgagc tcaagtaatt ttcttagtct gttatttctg ttaataaccc
     2161 ttctgtataa atcatttaaa tccgaagttg caaacctgcc gccgtcaagt ggaacaagag
     2221 gtcttaaatc cggtggaaga ataggaattc ttgttagtat catccactcc ggtttatttt
     2281 tagaattcct aaaagcttct actatacgca gtctctttga tattcttgct ctttttattg
     2341 gagatgaggt ttctctaagt tcaactctaa gtatttctga tagctcaacc agatcaatgg
     2401 actttaacat gtcccttaca gtttgtgcac ccattcctac cttaaaagac atgccatatt
     2461 cttccaaagc ccttctgtat ctctcttcac ttacggtttc agcaaatttc agaggggtct
     2521 gtcctgggtc tgtaacaaca taagactcgt agtataaaac cttttcaagg tcttttagag
     2581 tcatatccaa aagcattccg attctactgg gtatggatct taaaaaccat atatgagcta
     2641 ctggtgaggc aagctctata tgccccattc tctctcttct aacctttgtg gtaattactt
     2701 ctactccaca tttttcacaa gtatgtcccc tgtgttttag tcttttatat tttccgcaaa
     2761 gacattcgta atcttttaca ggtccaaata tttttgcgca aaaaagcccg gttctttcag
     2821 gtttaaatgt tctgtaatta atggtttcag gctttttaac ttcaccaaaa gaccaacttt
     2881 taatcttatc gggagacgca atagatattt ttactgaaga atagtcagaa ggattttttg
     2941 gtttttcaaa taatgtatct atatccatac tcataatgac ctccaatcaa atgtttttca
     3001 tttgattata aagcagatgt tgttgtttct tcttcctctt cctgtaattc aatatccagg
     3061 gcaagggcct gtaattcgtt ttgcagtact ttaaaagact cgggaattcc cggctcaaaa
     3121 tccatattac cttttacgat tgaatcaaat actcttgttc tacctattac atcatcagat
     3181 ttaaccgtga gaaattcctg tagagtataa gatgcgccat aagcttccag tgcccatact
     3241 tccatttcac caagcctctg tccgccaaaa tgcgccttac caccaagtgg ctgttgagta
     3301 acaagagaat aaggtcctat ggcacgtgcg tgaatttttt catctacaag atgatgaagt
     3361 tttagcatat acataacccc tactgctact ttttgatcaa acggctctcc ggttctacca
     3421 tcgaaaagaa tagtcttacc ttcaagtgga agtcctgctt gcgctaacat tgatttaatc
     3481 tcagactctt cggttccatc aaacacagga gtctttaccg gtattccttc atcatatttt
     3541 tcaatcagtt ccaatacctg ttcgtcgttt aattgtttta tatatttatt tacgttgggc
     3601 tcatcaaata tatccattag ctttttctta agaacctctg ggctgtattc tgtttgcata
     3661 tattcttgta tctgattgcc aagttctttt gcggcccagc ccaggtgagt ttcgagaatc
     3721 tgcccgacgt tcatccttga aggtacacca agcggattca ataccatatc tacaggagtt
     3781 ccgtccggta aataaggcat gttctctaca ggaactatat tagaaacaac acctttgttt
     3841 ccatgccttc ccgccatttt atctccaaca gaaagcttcc ttttaacagc aacatatact
     3901 ttgacaactt tcagtactcc cgggggcagt tcatcgggtt ttgatatttt ggtaagttta
     3961 ttgtcataga ccatttttat aaggtctgtc tgttcgagtg ttctttctat aacctttttt
     4021 acttcgtgct cgattgattc actttctttt acgcttatat ccgcaagttt ttcaaatggt
     4081 attgtatcaa gaatttcatc tgtaatggta tccccaggtt ttagcactgc ctttctgttt
     4141 acgtttagtt ttgatgcaga tgtttttccc gaaagtttag tcttgatttt atctacagag
     4201 tcttctttta aaatcttaat ttcatcatct ctgtcctgtt taagtttagt aacctcaaaa
     4261 tcttcgatgc tttttgctct ctcatctttc tcggcaactc tcgataccag catcttcaca
     4321 tctattacgg ttccatttac ccccggtgaa gcctttaaag aagtatcttt tacatcgccg
     4381 gctttatctc caaagatcgc tctaagtagt ctttcttctg gtgagagctg tgtttcacct
     4441 ttcggtgata tcttaccaac aataatgtct cctgctctta cctcagcacc aattcgaatg
     4501 attccacttt catctaaatt cctaagcgca tcatcaccaa catttggtat gtcccttgtt
     4561 atttcttctc ttccaagctt ggtttctctt gcaatacact caaactcttc gacatgaatt
     4621 gaggtaaaac tgtcttcttt tacaagtctt tgacttataa ggatggcatc ttcaaagttg
     4681 tatccacccc atggcataaa tgctacaata gcattttgtc caagggcaag ctcaccatac
     4741 tgagtagatg ggccatcggc aataatctgc cccccactta ttttttcacc cttcttaact
     4801 attggtattt ggttccagca tgtattttga ttagattttt gaaattttaa taaattatac
     4861 aactccacac cggcttcagc tccgttttcc tcttctattc ctcttataac tatctttgaa
     4921 gcatcaacaa actctacaat cccatctctc ttggcaacca cagtcactcc tgaatctttt
     4981 gccacgatgt tttcaaatcc cgttcccaca agtggggctt ttgacttaat tagaggtaca
     5041 gcctgcctct gcatgttcga tcccataaga gccctgtttg catcatcatg ctccaaaaaa
     5101 ggaatgagtg atgctgaaac agaaacaagc tgtgcggggg aaacatccat tagctgaaca
     5161 ttttcagcct cagttattaa aaactctccc ttgtgtcttg aagatattaa atcgttttta
     5221 aattttccat tttcatcaat aggcgcatta gcctgagcaa taatgtactg ttcttcttca
     5281 agcgcgttta aatatcttat ttccttagta actacagcat tttctacaat tctgtatggt
     5341 gtttgaataa acccgtggtc atttatcttg gcatatgtac taagacttga gattagacca
     5401 atattaggcc cttcaggtgt ttcaatagga cagattcttc catagtgaga tgaatgtacg
     5461 tctctaactt caaaacctgc tctctctcta gtaagacccc ccgggccaag agcgctaagt
     5521 cttcttttat gtgtgatctc tgatagcgga tttgtttgat ccataaactg tgaaagctgt
     5581 cctgtagtaa aaaattcttt taccactgaa atcactgtct ttggaatcac aagctcactt
     5641 ggcatgaggt tttccactgc ctgatatccc attttttcct taacagatct ggcaagcctt
     5701 tccagtccat ggtaaaacct gtcctctaca agttctccca ctgttctaac ccttctgtta
     5761 cccagatggt cgatatcatc agttgatccc ctgccgcctc ttaaatttag tatatatctt
     5821 acggattcga gaatatcctc tttggtaaga gtcattgttt cttcagatat tccatggtct
     5881 agcttataat ttattttaag ccttcccacc tgagacaatc tatatgtatc aggattgaag
     5941 aacatattat taaaaaatgt ttcagcaaca ttatatgtag gaggatcagt tggtctgaac
     6001 tttttataaa tatctattat agcttcttca gtatttgcaa ttttatcaac aagaatagta
     6061 tttctaagag atgcaaccac tgaaacgtta tcaatataaa atagtttaaa ttcctttact
     6121 ccagaagact ttagaacttc aagtttttct tcgtcaatct cttcattaaa gtttaatatt
     6181 atttcatcat tagcgtcttt tagctcatca gcagatatcc ttcctattat gtcagtagac
     6241 aaaataggta tgcggtcaag acctgcggat gttactctat caatagaatt ctttacaaac
     6301 cttctacctt ttttcactaa caccttagat gtattaggat caactatatc tacactacag
     6361 cgctgatagg ataaaagctc cgggtcaatt gacttatgga catcatctcc atccatataa
     6421 acagtttcag aagggtagta ataatacata atctcacggt tgctaagacc aagagccttt
     6481 aggagcacag atgaaggaaa tctcttcttt ctatcgattc ttacctgcag taaatccttt
     6541 gcatcaaact caaaatcaag ccatctgccg tcctgaggga ctatccttgc agagtaggta
     6601 gatctgcctg ttgttgtatc ctttgcagat acctgatcaa aaaacacacc tggggagcgg
     6661 tgaagttggt ttacaattac cctttcagta ccatttacga taaatgcacc gttttcggtc
     6721 ataagaggaa tttcaccaaa atatacttcc tgttctttta catctcttat cgatttttct
     6781 tcaggggcat cttcagggct ttcccatatt accagtctga atgttacata cagcggagca
     6841 acataggtat atcctttcag tctgcattca agtggttcgt gcttgggttt ttcaaacctg
     6901 tatttaacat attccaaagt tgctttctca ttgtaatctt caacaggaaa gacaagctca
     6961 aacgctcttt gaataccctg attttgaatt tgctcaggat caacgtcaat ctgcagaaat
     7021 tcaagataag atttaatctg aacatccagc aaatgtggat tttctaaaat aggtggtatc
     7081 tttgaaaatg attttctaaa tcggagtgtg tttgcaccat ttaaattatg actcaattct
     7141 acacacctcc cctgttagtt tttggataaa aaacgtaaaa gttataacaa atattctgta
     7201 gattaatgta aaagataggc ttattgtact tctactcctg caccagtttc ttctaatttc
     7261 tttttaatat cgtcggcttc ttctttagaa aggccttctt taattgcttt gggtgcagat
     7321 tctaccagtt cctttgcttc ttttagtccg agagcggtaa tttctcttac agctttaata
     7381 acctgtattt tattatcacc aatcgattgc agtacaacat tgaattcggt cttttcagca
     7441 cctgcggcgg cttgatcatc tgctccacct acgggggctg caactgcaac tgcgggtgct
     7501 gctgctgtaa cgccaaattt atcttctact tcttttatta actctgaaag ctcaagcatt
     7561 gttgaatttt caaggtatgt aagaacatca gctctagtaa cttcagccat tttagtttcc
     7621 tcctaaggat aataattgtt gatttttcat ttaattttta aatttaagac tcttttgtat
     7681 ttttaacctc atttaacgca taaaccagtt tagtctgcat atttttaata gtagcaagaa
     7741 aaccagtcag tggactgttt aacattccaa caaactgccc gagcattgct tctctggaag
     7801 gtagttttga taagcttgta atctcggact cacttagaac gctgccatca actaagcctc
     7861 ctttgagttt tacctgagga agtgttttaa ttgaatcgac aaatgctttt gcgatagaaa
     7921 caggatcttc tttggatatt gctactgctg taggccctat aaacatatca gctatttttt
     7981 cgacctcact gccttcagcg ccgtatttca gtaccgagtt tttaactacc gaaaactgag
     8041 cattagcttt ttttacgctt cttctaagat cctgtatctc tttaacagta agccccttat
     8101 agtcgagcac aaatacagaa gatgcctcct taaacttttc accgtaatct tttgcaagat
     8161 caattttctg ttgtttacta agcatttaat aactccctta tatatttcta tgcagcaaaa
     8221 tcaaatgttt tgatctgttc tttaatatca ttaatatcta cttttatacc aggccccatg
     8281 gtgtttgata taactatttt tctaagatat acaccttttg aagcagatgg tttcattttc
     8341 ataatagcat ctagcaatga caagaaatta gcttttaact tatctacaga aaatgaagat
     8401 tttccaaacg gagcatgtac aacgccgccc ttatcatttt ttatctctat tcttccagcc
     8461 tttgaatcct cgactgcttt ttttacttca aaagtaaccg tgccaacctt aggacttggc
     8521 attagccccc tgggaccgag cactttacca agtttaccaa cactgctcat aacatccgga
     8581 gtggctacag ctacatcaaa atcaaaccag ttctcatttt gtattttttc aacaagatca
     8641 tcggaaccta cataatcagc ccctgcatcc tgagcctctt ttactttctc accctttgca
     8701 aaaacgagta ctttgatatc cttgccaact ccatgaggaa gaacttttgc tcctctaagc
     8761 tgttgattag cctgccttgg atcaatgcca agtttaatag caagctcgac agtttcatca
     8821 aattttgcta cttttgtctc cgatataagc ccaagcgatt catccacgct atacaactta
     8881 tcaatgtcta ctttttgttt agctgctatg taatttttcc ctgatgccat ttcttgctcc
     8941 taattttaat attactgtat ttcaagcccc atacttcggg ctgtaccttc gataatcctc
     9001 attgctgatt ctattgtatc agcattaagg tcttccattt tggtattagc tatttcttga
     9061 atctgatctt tagtaacttt gcctgcttta actctaggaa cctcactgga tccttttttg
     9121 atttttgctg cttttttaag taaataggaa accggtggag tttttacaac aaacgaaaac
     9181 gacctgtcag catatacgat aaccgtaacc ggcagaaggt ctggtgaatt ttgtgtttgt
     9241 gcattaaatg ccttacaaaa ctccataatg tttacccctc tttgacccag agcaggtcct
     9301 acaggaggtg atggtgatgc cttgccgcct tcgattaata gtttaatttg tcctacttct
     9361 tttttcatag ctataatttc tccacttgat taaagtctag ttcgattggt gtagttcttc
     9421 caaaaatagt tacattaacc tgtactttag ctttatcgga attcacctct tctatcactc
     9481 cagtaaaatt agcaaaagga ccctcattaa ccttaacagt ctcacctttt tcataaaaaa
     9541 tcttaggttt gggtttaagg cttccttcct caacctgagt tttgatcttc attacttcat
     9601 tctcatcaat gtcaggaaca gagttggggt caattttacc gtcttttaac tttcctccta
     9661 caaaacctat taccttggga atatccctta gaaaatgcca ggttacttca tttaattcca
     9721 tatttactag tatataacca gggaagaact ttctaattga agtttttctc ttgccatctt
     9781 tagcagcttc aacaatggtt tcagtaggaa taagcacttc agaaatagaa tcttcgagat
     9841 tttccgcttt agccctgtcg gttatatagg tttttaccct gtcttcaaat cctgtgttag
     9901 tatgtacaac ataccatctt tttgacatat tattattctc tccgtaaagc tttaagctaa
     9961 tacgtactta accaccatcg aaaaaagcga atcaacacca aaaagaaaac caatgagtat
    10021 tgttgatata acaactacag ccagtgttga tttaagtgta tcatttaaaa atggccaggt
    10081 aatttttgtt atctctacct ctacatcttt taaaaaatcg cttgttctat tctttagatc
    10141 gttaaaattg ctcatattta ataattaata aattttattc agggcaggag ggactcgaac
    10201 ccccaaccgc ttgatttgga gtcaagtgct ctagccaatt gagccactgc cctttatttt
    10261 gtttccttat gctgtgtatg cttcttgcac catctgcaat attttttaaa ttcaagcttt
    10321 tcactatggt ttcttttatt cttgattgta gagtaattcc ttcttttaca atctccacag
    10381 gcaagcgtta catttactgt gttatcaccc attttttatc attactccaa tattttagtt
    10441 accacaccgg cacctactgt tctgccgcct tctcttattg caaacctcag cttctcttcc
    10501 attgccactt ctgttattaa ctccacaacc atgttga
//
LOCUS       DBNGSV010000005         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1108964, whole genome shotgun sequence.
ACCESSION   DBNGSV010000005 DBNGSV010000000
VERSION     DBNGSV010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1108964"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="AC159U_00415"
     CDS             complement(<1..1402)
                     /locus_tag="AC159U_00415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR3335551.1"
                     /translation="MSVYKKYRNKKNNNNKNQTKTKSSLSTTTRPDSNLIGTVKKVGK
                     NYVFVPRKLNRFYKSFDIDKNGSKPKRNTLYSAKVIQSPKKGIIVRLENEIGSAGSLD
                     TERESLLHEYGMHEGFSEIVKKSVKHIKLSISERELNRRTDLRDKLIFTIDGEKARDY
                     DDAVGIEKLKTGYKLWVCIADVSHYVKPSSELDLEAYRRATSVYLSKKVVPMLPERLS
                     NNLCSLVPNEDRLTKTAEIVFNNDGTIKEYSVYRSVIKSKYRLTYNKVTEILNGDYRL
                     DKKDAKLLKSLQLMEQLYKKVKKLRLDAGYIDLNIPEAQIVEDEDGNVVDVTKLKRES
                     SHELIEFFMITANNVVASYINKTGSASIYRIHDSLKPDSMEELKDKLKGLGYNKKIKN
                     KVKASEVQEILNYFKDTPKEYAANLFILRSMSRAIYGTKDTGHFGLALDYYTHFTSPI
                     RRYPDLIIHRLLDSLID"
BASE COUNT          386 a          300 c          262 g          562 t
ORIGIN      
        1 tatcaatgag actgtcgagc agcctgtgta ttataagatc aggataacgc cttatgggag
       61 aggtgaagtg agtgtaataa tctagcgcaa ggccaaaatg ccctgtgtcc tttgtgccgt
      121 aaattgccct gctcatcgag cggagtataa acagattggc tgcatattcc ttgggggtat
      181 ctttaaaata gttaagaatt tcctgaacct cgctagcctt aaccttgttt ttaatctttt
      241 tattatatcc aagaccctta agtttatcct taagctcctc catggagtct ggttttaggg
      301 aatcatggat tctgtagata gatgcagaac ctgttttatt aatatacgat gccacaacat
      361 tgtttgccgt gatcataaaa aattctatta attcatgaga agactctctt ttaagcttgg
      421 taacatccac tacatttcca tcttcatcct ctacaatctg tgcttcaggg atgttgagat
      481 caatatagcc tgcatccagc ctgagctttt taactttctt gtataactgc tccatgagct
      541 gcaggctctt tagcagcttt gcgtcttttt tatcaagcct ataatctccg tttaatattt
      601 ccgttacctt attgtatgta agtctgtact tactttttat aacgcttctg taaaccgaat
      661 attctttaat agttccgtca ttattaaata caatctcggc agtctttgta agcctgtcct
      721 cgtttggcac caggctgcaa agattattgg aaagtctttc gggaagcatg ggcactactt
      781 tttttgacaa atacacgctt gtagccctgc ggtaagcctc taaatccagt tctgatgagg
      841 gttttacata atgtgataca tcggctatac aaacccacag tttataaccg gttttaagtt
      901 tttcgattcc aacagcatca tcatagtccc ttgccttctc cccatcaatg gtaaatatca
      961 gtttgtctct taagtcggtg cgtctattaa gttccctctc agaaatggag agtttaatat
     1021 gctttacact cttttttact atttcactaa acccttcatg cataccgtat tcatgcagaa
     1081 gtgactctct ttcggtatca agtgaacccg cactgccaat ttcattctca agtcttacta
     1141 ttattccctt tttggggctc tgtattacct ttgcgctgta cagagtattc cttttgggtt
     1201 ttgatccatt tttatcaata tcaaagctct tataaaacct gtttaacttg cgcggaacga
     1261 atacatagtt tttgccaacc ttttttaccg tccctatgag atttgaatca ggcctggtgg
     1321 tagtagataa agaactttta gtttttgttt ggtttttatt attattgttt tttttgtttc
     1381 tatatttttt gtatacactc atattggttt tacttttacc ccgtggatta ttgcccctag
     1441 atatttaaat tgaaatatta atgatttgtg tggatatggt tataagaatg tcttgtattt
     1501 atgaataaac
//
LOCUS       DBNGSV010000006         2188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_277788, whole genome shotgun sequence.
ACCESSION   DBNGSV010000006 DBNGSV010000000
VERSION     DBNGSV010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_277788"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2188)
                     /locus_tag="AC159U_00420"
     CDS             complement(<1..>2188)
                     /locus_tag="AC159U_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3335552.1"
                     /translation="RSRVLEYLNQVSGKLPEGVNPTLGPDATGVGWVYQYALVDESGN
                     NDLSDLRTFQDWYLRYWLESVPGVAEVASVGGFEKQYQVEIDPNKLVAYDIPLNKIIS
                     AIRRSNNDVGGRVVEFASTEYFVRGRGYIKSTSDIEKIPVGVGKDGTPIYIRDIADVH
                     MGPEIRRGLAELNGKGEAVGGIVVMRYGENALEVISNIKAKIQEIQPSLPKGVKIVTT
                     YDRSGLINRAIDTLKGKLIEEIIIVSIVIIIFLFHLRSAAVPILTIPFAVLMAFIPMF
                     YLKNTSNIMSLGGIAIAIGAMVDASIVLVENVHKRLEGKILGDNQQKRTEIIIDAMKE
                     VGKPIFFALLIITISFLPIFTLEAQEGRLFRPLAYTKTFSMFFASLLAITLTPALIVL
                     FIKGEIKSEENNPINRFLIKMYDPVVRFALKFKTAVIVVSVLILIVTYLPYKKLGEEF
                     MPPLNEGSILYMPTAVPGMSITEASKILHIQDKMLMKFPEVQTVFGKIGRAKTATDPA
                     PLSMVETVITLKPEKEWRAGMTWDKLISEMNTTMKFPGMANIFWMPIQTRTEMLSTGF
                     RSNLGIKVFGPDLNIIENIGIEIEKILTEVPGTRSVFADRVTGGYFMDFNISRERAAR
                     YGLTVGDVEDVIESAIGGKNISHTVEGQERYPINVRYQRGFRNDLEALGSVLVSSPTG
                     VNVPLSLLTDIKFTTGAPQIRNEDGQKVGFVNVDVSDGDYAGFVDKA"
BASE COUNT          601 a          507 c          378 g          702 t
ORIGIN      
        1 gctttatcaa caaatccggc atagtcgccg tctgagacat cgacatttac aaagccaact
       61 ttctgcccgt cctcgtttct tatctgtggt gcgccggttg taaattttat atccgttaat
      121 aatgaaagag gaacatttac tcctgttggg cttgatacaa gcacactgcc aagcgcctca
      181 agatcgttcc tgaaccccct ttgatatctg acattgatag gatatctttc ctgaccttct
      241 acagtgtgtg atatattctt acctccaata gcactttcta ttacatcttc gacatcaccc
      301 accgtgagac catacctggc tgctctctcc cggcttatat tgaaatccat aaaatatccg
      361 cctgtaaccc tgtcagcaaa aacacttctt gtaccaggaa cttcggtaag aattttttca
      421 atctcaattc cgatattttc aataatattc agatccggac cgaatacttt aataccaaga
      481 ttacttctaa aaccagttga tagcatctcg gttcttgttt gaatgggcat ccagaaaata
      541 tttgccattc ccggaaattt cattgtcgta ttcatctcag atataagctt gtcccaggtc
      601 attccagccc tccactcttt ctccggcttt aaagtgataa ctgtttcaac catactgagc
      661 ggagctgggt cagtagctgt cttggccctt ccgattttcc cgaatactgt ctgtacctca
      721 ggaaatttca tgagcatttt atcctgaata tgaagaattt ttgatgcttc tgttattgac
      781 atccccggta cggctgttgg catataaagg atgctgcctt cattaagagg cggcataaat
      841 tcttcgccaa gttttttgta gggaagataa gtgactatta atatcaggac agagacaact
      901 attacagcag ttttgaattt tagagcaaat cttacaacag gatcatacat ttttattaaa
      961 aacctgttaa tcggattgtt ttcttcagat ttaatctcac ctttaataaa caaaacaata
     1021 agcgctgggg taagagtaat agcaagaagg gatgcaaaaa acatagagaa tgtctttgta
     1081 tatgcaagtg gcctgaatag ccttccttcc tgtgcttcca aagtaaatat tggcagaaat
     1141 gatatcgtta ttatgagaag agcaaaaaat atgggctttc ctacctcttt catggcatct
     1201 ataattattt cggttctctt ttgttgatta tcaccaagaa tcttaccctc tagcctctta
     1261 tgtacatttt ccacaagaac aatagacgca tcaaccatgg cgccaattgc tatggctata
     1321 ccgccgaggg acatgatgtt tgatgtattc ttaaggtaaa acatcggtat aaatgccatt
     1381 agtactgcaa aggggatagt tagaatggga actgctgcgc ttcttagatg aaatagaaat
     1441 ataataatta ctatgcttac aatgatgatt tcttctatta atttcccctt tagtgtatca
     1501 attgcgcggt ttattagacc ggagcggtcg tatgtggtta caattttaac tcctttgggt
     1561 aaagatggct gtatctcttg tatttttgcc ttaatgttac ttatcacttc taaggcattt
     1621 tctccgtagc gcattactac tataccgcct accgcctcac ctttaccgtt taattcggca
     1681 agccctcttc tgatttcagg gcccatatga acatctgcaa tatctcttat ataaatcgga
     1741 gtaccgtctt ttcctactcc taccggtatt ttttctatat ccgaagtgga tttgatatac
     1801 cccctgcctc ttacaaaata ttctgtcgag gcaaattcta ctactcttcc ccccacatca
     1861 ttattgctcc ttctaatagc agaaattatt ttattaagcg gtatatcgta agctacaagc
     1921 ttattgggat caatctctac ctgatactgt ttttcaaatc ctcccacgct tgctacctct
     1981 gctacacccg ggacagattc aagccagtag cgcagatacc agtcctgaaa agtgcgtaaa
     2041 tcagacagat cgttatttcc gctttcatcc acaagcgcat actgataaac ccatcccaca
     2101 cccgtagcat ccggacccag tgtgggatta actccttcag gaagctttcc cgagacctgg
     2161 ttcagatact ctaacaccct gcttcttg
//
LOCUS       DBNGSV010000007         5742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1732257, whole genome shotgun sequence.
ACCESSION   DBNGSV010000007 DBNGSV010000000
VERSION     DBNGSV010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1732257"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC159U_00425"
     CDS             complement(<1..362)
                     /locus_tag="AC159U_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335553.1"
                     /translation="MDNYHSLPKRQLKKHFNNFDELSDELKEFIESVDSAYTEFDSDR
                     TMLERSLDLSSQELLQANTDMRAVFNASPDLFLWIDIDGVIFDTKGGDKSDLYLPLDK
                     LIGKKIQNVPPKILGNKF"
     gene            complement(366..1505)
                     /locus_tag="AC159U_00430"
     CDS             complement(366..1505)
                     /locus_tag="AC159U_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR3335554.1"
                     /translation="MKIEQSIWTKKKGWNPNIPGNLSKPPQLVLVFGSSNNINSKKLI
                     KEINNAYPDSQILGCSTAGEIIDNMVVDDSLTVTAIYFENTDIKSVKTNIKHASESFK
                     AGKSLAKSLNNNDLSHVLVLSEGLNIDGSELVKGLLSSLPANVTVTGGLSGDGDKFNK
                     THLLWNNKVYNNCIAAIGLYSKNLKIGYASLGGWDSFGPERIITKSRGNVLYELDGKS
                     ALELYKKYLGEHAEGLPATGLLFPLSLRETEEDIGVVRTILSVDEKDQSMTFAGDVPE
                     GSFARLMKANFNRLIDGASGAAKTSLEAKGSDPTDLAILISCVGRKMVLKQRIDEEVE
                     AVRDILGSEAVFTGFYSYGEISPFTPDAKCELHNQTMTITTLSEK"
     gene            1651..2346
                     /locus_tag="AC159U_00435"
     CDS             1651..2346
                     /locus_tag="AC159U_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3335555.1"
                     /translation="MNQNILVIIPAFNEQNSIGSVLTDIPKDLVSEVVVVNNNSTDDT
                     EKVAVSLGATVLSENRRGYGYACLGGIEYAKSKPAKEKPDVIVFLDGDYSDFPQEMTE
                     VVRPITEDGFDMVIGSRTIGNREAGSMLPQALAGNFIATKLIKLFYGYSFTDLGPFRA
                     IKFDKLLELDMRDTTYGWTVEMQIKAAKKKLKCTEVPVFYRKRIGVSKITGTIEGTIK
                     ASYKILWTIFKYL"
     gene            complement(2353..2604)
                     /locus_tag="AC159U_00440"
     CDS             complement(2353..2604)
                     /locus_tag="AC159U_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335556.1"
                     /translation="MRPVQYLSKEYLEQCKKMTPEQIIQFLDDFRTLHGSVDRSKSKL
                     ISIKIPENLLNAFKTRSKLVGVPYQTQIKKLMTEWIKEG"
     gene            complement(2601..3065)
                     /locus_tag="AC159U_00445"
     CDS             complement(2601..3065)
                     /locus_tag="AC159U_00445"
                     /inference="COORDINATES: protein motif:HMM:NF039330.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HIR3335557.1"
                     /translation="MFIYKLVKKLAENNIEYAIAGGYAVAFHGAVRGTVDIDLIIKLE
                     KESFLKIEKTLEQIGLQSRLPIKAEEVFNFREEYINNRNLIAWNFINLNNPIESVDIL
                     LLEDLKKFKTININSNNNILKVVSMNDLIKMKKRSGRPQDIEDIKILRSLKQ"
     gene            3172..3870
                     /locus_tag="AC159U_00450"
     CDS             3172..3870
                     /locus_tag="AC159U_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015120671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3335558.1"
                     /translation="MSNPVCAIIGIGPGNGLSFAKKFSKEGYSVALCSRNLNRLQEYA
                     DQIDNAHAFEYDVTDAEAADTVFSQIREKLGPVSTLIYNAGAGDFSNFDDSTLKSFQR
                     AWEVNAGGLFTAVKAVLADIRSCKGNIVVIGATASLRGGINTVPFASAKSAQRSLAQS
                     LSKSLGPEGVHVSYVIVDGMINLESTRRNMPGKPAEFFMIPDDIADSVYFLTEQKESA
                     WTFELDLRPFGEKW"
     gene            complement(3845..4873)
                     /locus_tag="AC159U_00455"
     CDS             complement(3845..4873)
                     /locus_tag="AC159U_00455"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR3335559.1"
                     /translation="MKKVISISEQFAIGKVLDVSEYGSGIVNDTYLVENTSGQKFILQ
                     KQHELIGKEVLEDIEAVTKRLESSDILTPKLIRNNSGELYIEKEGYIWRVLTFVEGKS
                     YEKLSDLQLVKSAGEIIGNFHCSLIGFQYHFKHEIPQFHDTEYIISKLQNITSKNQGN
                     KEYQQLNQLSDYINNEHKSVRGVLDHLPDRIIHGDLKLSNIRFNENQTKAICLLDLDT
                     LGRNKIVIDLGDAIRSFCNTQNKFDLEIFRSLMEGYFNSVSFLTHEEIDHIPAGIKTI
                     VLELAARYITDAIEEKYFKLEEMKFPTLYVQNTTKAQSLISLYEDLLNKIDSVQDIIS
                     TTTSLQKA"
     gene            4972..5445
                     /locus_tag="AC159U_00460"
     CDS             4972..5445
                     /locus_tag="AC159U_00460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3335560.1"
                     /translation="MRKETKKTIEVVHTELVLPGDTNHYGTIFGGKTLAMMDMTGALA
                     AMQFCNEDVVTASFESIDFKSPIKQGDITEVKAKVIYTSNTSMVVKIDVYKLGKFHSR
                     KSYTCGGYATFVAIDSNGEPRSIPQLKIATKEEKRLVEIGKQIKQKAMERAKSSN"
     gene            complement(5455..>5742)
                     /locus_tag="AC159U_00465"
     CDS             complement(5455..>5742)
                     /locus_tag="AC159U_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335561.1"
                     /translation="DAVGDTARIKVWSDNTTYLLTYADVNNELRFALEPDNASYICQK
                     RWLLESNGKKTKLTLIDIYTESGKQSQESLDKQVADWEKKLAKLKELAEAS"
BASE COUNT         1728 a         1001 c         1067 g         1946 t
ORIGIN      
        1 ttaaatttat taccaaggat tttgggtggg acattctgaa ttttcttgcc aatcaatttg
       61 tcgaggggta agtaaaggtc tgatttatcg cctccttttg tatcaaagat gaccccatct
      121 atatcaatcc ataaaaagag atctggagaa gcattaaaaa cagctctcat atcggtattt
      181 gcctgcagga gttcttgtga gcttaagtcc aaagaacgtt cgagcattgt tctgtcagaa
      241 tcaaactcgg tatatgcaga atcaactgat tcaataaact cttttagctc gtcggataat
      301 tcatcaaagt tattgaagtg tttttttaac tgtcttttag gaaggctgtg ataattatcc
      361 atttattact tctctgaaag tgtggtaatg gtcatagttt gattatgcag ctcacacttg
      421 gcatcaggag tgaaaggaga aatttctcca taagaataga atccggtaaa aacagcctca
      481 cttcccaaaa tgtctcttac ggcttcaacc tcttcatcaa tccgctgttt caagaccatc
      541 ttcctgccca cgcaactaat taatattgca agatcggtag gatctgatcc tttggcttcg
      601 agggatgttt ttgcagcccc cgaagcgccg tcaataagcc tgttgaaatt ggctttcata
      661 agccttgcaa aagaaccctc gggaacatca cccgcaaatg tcatgctttg atctttttca
      721 tctacagaga gaatggttct tactacacca atatcttctt cagtttctct cagactgaga
      781 ggaaatagta aacctgttgc cggaagcccc tctgcatgct ctccaaggta ttttttgtat
      841 agttccagag cagatttacc atcaagttca tatagtacat ttcccctgga ttttgtgata
      901 attctctccg ggccaaaaga atcccatcca ccaagtgatg catagcctat tttaaggttt
      961 ttactatata gcccgatagc tgcaatacag ttattgtaaa ccttgttgtt ccataataga
     1021 tgtgttttat taaacttgtc tccgtctccc gaaagccccc ctgtaacggt gacgttggcg
     1081 ggtaaactgc ttagaaggcc ttttacaagc tcacttccgt caatattcag accttctgat
     1141 aaaaccagaa catgtgaaag gtcattatta tttagtgatt tagccaggga tttacctgcc
     1201 ttaaagctct cagatgcatg tttaatattg gttttaacag attttatatc agtattttcg
     1261 aaataaattg ctgtaacagt aagtgaatca tccactacca tattgtctat tatttcacct
     1321 gcggtagagc atccaagaat ttgagaatca ggatatgcat tatttatttc tttaataagc
     1381 tttttgctat ttatattgtt tgagcttcca aagactaata ctaactgagg tggcttggag
     1441 agatttccgg gaatatttgg gttccagcct tttttctttg tccaaattga ttgttctatt
     1501 ttcatctcaa actaaattat ataaattttc ttgatattta tcaatttata tcttgtttca
     1561 ttactaaaat cagttagttc tatctcttat actttcagat tcagttatgt aaatccggtt
     1621 atatttaacg ctaacttatt aaataaagag atgaaccaaa atattctggt aataattcct
     1681 gcattcaatg agcagaattc cattggaagt gtgttaactg atattccaaa ggatttggtt
     1741 agtgaagtcg ttgttgtaaa taacaactcc accgatgata cagaaaaagt tgccgtgagt
     1801 ctaggtgcta cagttcttag tgaaaataga agagggtatg ggtacgcatg cttaggaggt
     1861 attgaatacg caaaatctaa gcctgcaaaa gaaaagccgg atgtgattgt atttttggat
     1921 ggggattact cagacttccc acaggaaatg acagaggttg taagacccat tactgaagat
     1981 ggttttgata tggtaatagg ttccagaact attggtaata gggaagccgg aagcatgctt
     2041 ccgcaggcat tggctgggaa ctttattgct acaaaactta taaagttatt ctatggatat
     2101 agctttacag accttggacc ttttagagcg attaagtttg ataaactttt agaattagat
     2161 atgcgggata caacatacgg ctggactgtt gagatgcaga ttaaagccgc caagaaaaaa
     2221 ctcaagtgta ctgaagttcc tgttttttac aggaagagaa tcggtgtgtc aaagataacg
     2281 ggaactatag aaggaactat taaagcaagc tacaaaatac tctggacaat atttaagtat
     2341 ttatgatatt gactacccct cttttatcca ttcagtcatg agctttttga tctgagtttg
     2401 atatggtact cccacaagct tagacctggt tttgaatgca tttaaaagat tttctggaat
     2461 tttgatgctt attagcttgc tttttgatct atcaactgag ccatgaagag ttctaaaatc
     2521 atctaggaat tgaatgattt gttctggtgt catttttttg cattgttcta aatattcttt
     2581 tgatagatac tgaactggtc tcattgtttt aagctccgta gaatttttat atcttctatg
     2641 tcctgaggtc ttccacttct ttttttcatt ttgataagat cattcataga aactactttt
     2701 aaaatattgt tattagaatt gatatttatt gttttaaatt ttttaaggtc ttctaatagt
     2761 aatatgtcca cagattctat tggattattt aaattaatga aattccaggc gataaggttt
     2821 ctgttattaa tgtattcttc tctaaaatta aatacttctt cagcttttat tggtaatctc
     2881 gattgtaaac caatttgttc taaagttttt tcgattttta agaaactctc tttttctaat
     2941 ttgatgatca aatctatatc aactgtacct ctaactgctc cgtgaaaggc aacggcatat
     3001 ccacctgcaa tagcatattc aatattgttt tcggctagtt ttttaactaa tttatatata
     3061 aacatatggg tatataccag tatataccca tatttaagta aaataaagag tacttcaagt
     3121 tgattaactt attataatat ataatacata aaacaattgg agggtgctta catgtctaat
     3181 cccgtatgcg caattattgg tatcggcccg ggaaacgggc tgtcatttgc caagaagttt
     3241 tcaaaagagg gttattctgt tgctctttgt tctagaaatt tgaacaggct gcaggaatat
     3301 gcagatcaaa tcgataatgc gcatgctttt gaatatgatg tgacggatgc tgaagcagcg
     3361 gatacagttt tttcacaaat cagagagaaa ttaggtccgg tcagcacatt aatatacaat
     3421 gccggtgcgg gggatttttc aaactttgat gattccacac ttaaaagttt tcagcgtgca
     3481 tgggaagtaa acgccggtgg actgtttaca gcagtaaaag ctgtactggc tgatatcaga
     3541 agttgtaaag ggaatatagt tgtaattggc gctacggcct cactgcgagg tggtattaat
     3601 acagtaccgt ttgcctctgc caaatctgcg cagagaagtc ttgcacagtc actctctaag
     3661 agtttgggcc ctgagggagt gcatgtttcg tatgtgattg ttgacgggat gataaatttg
     3721 gaatccacac gcagaaatat gccaggtaaa ccggccgagt tttttatgat cccggatgat
     3781 attgcagact cagtatattt cttaactgaa caaaaagaaa gtgcctggac atttgagctg
     3841 gacctcaggc cttttggaga gaagtggtag tggaaatgat atcctgaaca gagtcgatct
     3901 tattcaataa atcttcatat agactaatta atgactgagc ttttgtggta ttctgtacgt
     3961 aaagggtagg gaatttcatc tcttcaagct tgaaatattt ttcctcaatg gcatctgtta
     4021 tatacctggc tgccaattca agaactatag ttttaatgcc tgcaggaata tgatctattt
     4081 cctcatgagt aagaaaagat acagagttaa agtatccttc cattagactt ctaaagattt
     4141 ccagatcaaa cttattttga gtattacaaa aacttctaat tgcatcgcca aggtctatga
     4201 caattttgtt tcgtccaagt gtatcaagat cgagtaaaca aatggctttg gtttggtttt
     4261 cattaaatct tatattgcta agcttgaggt caccgtggat aatgcggtct ggaagatgat
     4321 ccaatacacc tctaacactt ttatgttcat tatttatata atcagaaagc tgatttaatt
     4381 gttgatattc cttattaccc tggtttttag atgtaatatt ttgtagtttt gaaattatgt
     4441 attctgtatc atgaaattga gggatttcat gtttaaagtg atattgaaat ccgattaatg
     4501 aacagtggaa attacctata atttcacccg cactttttac taactgcaga tcggaaagtt
     4561 tttcatatga tttcccttca acaaaagtga gcactctcca aatatagcct tccttctcaa
     4621 tatataattc tccgctatta tttctaatca attttggggt taaaatatcg ctagattcca
     4681 atctttttgt gacagcttca atatcttcta aaacttcttt gccaattaat tcatgctgct
     4741 tttggaggat aaatttttgc ccggatgtgt tttctacaag atacgtatca tttactattc
     4801 cgctgccgta ctctgataca tctaatactt tgccgattgc aaattgttct gatattgaga
     4861 taactttttt catataaatt aaatattata ctaaactaaa ttgtttttca gtttaatctg
     4921 gtagtataag aaactcaatt taagatagat catttttagg aaagaaataa aatgagaaaa
     4981 gaaaccaaga aaacaattga agtggttcac actgagcttg tacttccggg ggatactaac
     5041 cactatggga caatctttgg tggaaagaca cttgctatga tggatatgac tggagccctt
     5101 gcagccatgc agttttgcaa tgaagatgtt gtgacagctt catttgaatc aattgacttt
     5161 aaaagcccta tcaagcaggg tgatatcaca gaggttaaag ccaaagttat ttatacatct
     5221 aatacatcaa tggtagtgaa aatcgatgta tataagcttg gtaaattcca ttccagaaaa
     5281 agctatactt gtggaggcta tgcaactttt gtggcaatag attccaatgg tgagccacgc
     5341 tctataccgc agctcaaaat tgccactaaa gaagaaaaga gattggtgga aattggtaaa
     5401 caaatcaagc agaaagctat ggaaagggct aaaagctcaa actagaatta atgtttatga
     5461 tgcctcagca agttctttta acttagctag tttcttttcc caatctgcta cctgtttatc
     5521 gagactctcc tgactctgtt ttccggattc agtataaata tcgattaatg tgagctttgt
     5581 tttctttcca tttgattcaa gcagccacct cttctgacaa atataagatg cattgtcagg
     5641 ttcgagtgca aaccgcagtt cattgtttac atcggcataa gtcagcaaat atgtagtatt
     5701 gtccgaccag accttgattc tggcagtatc tccgacagca tc
//
LOCUS       DBNGSV010000008         7753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_970514, whole genome shotgun sequence.
ACCESSION   DBNGSV010000008 DBNGSV010000000
VERSION     DBNGSV010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_970514"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..506)
                     /locus_tag="AC159U_00470"
     CDS             complement(9..506)
                     /locus_tag="AC159U_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335562.1"
                     /translation="MKAIRILGKERMSESGASSQFSTLDFSFAEILHNIEELVSELYW
                     AHTGIIYSFPESLTQEQYNITKRNLEEYLLHEDKARSINYMHRGFIENFGQYIFSEWV
                     ELAGYDDIQKLKTFDPTDSDSIEENAVVFFRCVDAAYWAVYAKNEEIINILESNFKFT
                     ETIGT"
     gene            complement(522..2483)
                     /locus_tag="AC159U_00475"
     CDS             complement(522..2483)
                     /locus_tag="AC159U_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HIR3335563.1"
                     /translation="MGIAADIAIIIVAALIGGSAARMLRQPLIIGFILAGVAVGPYTG
                     FIEVSEVHNIELLAEIGVALLLFALGLEFSLKELNPVRNIALFGTPIQIILTTAFGAG
                     IGRLFDLDWNSSIWFGALISISSTMVILRTLMSQGRMGTLSSRVMIGMLIVQDLAVIP
                     MMIILPQLANLGSGISLIGIAALKGFTFLGVMILVGTKILPVLLSHIARWNSRELFLI
                     AITAIGLGVGYASFLVGLSFAFGAFIAGIVLSESDYGHQALSDIVPLRDIFGLLFFVS
                     VGMLLDPSFLISNIKTILLLVVLVMIGKGLIFSGISLLFNYRNVIPFALGLGLFQVGE
                     FSFVLARLGLSSGSISTELYSLILNTAVITMVLTPLISGLTGPIYTFFKGRLHYEEMD
                     TMNIPKSGLHNHIVIAGYGRVGKHISKILKLLKLDFVIIEFNYNRVLDAKNADMPVIF
                     GNIRQGVVLDAAKINDARLLIVTIPSILITQSVVSTVQSINPNLHIVARTEDIEHIKT
                     LHDMGVYEVVQPHFEAGLEITRQALLHLNVPAAEITKYEDAVRQDLYEPLYRINKEYK
                     EMADLKKAANMLDLSWINLADDSPLLGKTIRELDIRRKTGVSIVGILGSGGVNPNPGS
                     EYIFKAGDRLAVIGSHDQLEIFQNICESN"
     gene            2605..2832
                     /locus_tag="AC159U_00480"
     CDS             2605..2832
                     /locus_tag="AC159U_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin, RHH family protein"
                     /protein_id="HIR3335564.1"
                     /translation="MPTKNPRINIVLEKPLYNAIKKIAKKEGISISLKARDFIREALE
                     THEDALLDEIASEREKGFSKEKALSHEDIWS"
     gene            2845..3120
                     /locus_tag="AC159U_00485"
     CDS             2845..3120
                     /locus_tag="AC159U_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR3335565.1"
                     /translation="MAYSIIYHPDVKDDIKKFDSKIKQRVQDAIEQRLSTEPEKYGKP
                     LRKSLKGFWKLRVGDIRIVFKILKSEIVVLAILNRKEVYERINKRIE"
     gene            complement(3130..3711)
                     /locus_tag="AC159U_00490"
     CDS             complement(3130..3711)
                     /locus_tag="AC159U_00490"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3335566.1"
                     /translation="MKRICVFCGANKGNKPEYAEAARKLAEELVSHDIEIVYGGGRIG
                     LMGVIAERAIELGGKVIGIIPEALALKEVAHDTITDLIVVGSMHERKAKMAQLSNGFI
                     SLPGGIGTIEETFEMLTWSQLGFHSKPCGIINVLGFYDKLIEFLNHISEEKFFMDIYR
                     RMLIVETDPSALLGKFEQYKPPQVQHWIAEDEI"
     gene            complement(3807..4304)
                     /locus_tag="AC159U_00495"
     CDS             complement(3807..4304)
                     /locus_tag="AC159U_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3335567.1"
                     /translation="MKLEGSCHCKAVKFSVESQHPYPYMYCYCSICRKTAGGGGYAIN
                     LNGDYSTLKVEGKENITVYRAYLNDKDAKEDKKSPAERSFCRKCGSSLFVWDPRWPEQ
                     VHPFASAIDTELPTPPEKTHIMLEYKAGWVEVQKGSADKEHPEYPDESIAGWHERLGL
                     EDKSE"
     gene            complement(4323..5177)
                     /locus_tag="AC159U_00500"
     CDS             complement(4323..5177)
                     /locus_tag="AC159U_00500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3335568.1"
                     /translation="MDANNDFINCWNEILVPKWNRFRHILSGNGGVHSDMAFADFDIQ
                     PGDKVLDIGCGYGETCLDIGRIVGPEGEVLGLDCTQAFLDIANKERDEAGLTNVRFEL
                     GDAQTYDLPENYYDVVYSRFGVMFFQNVVYALKNAHKTLKPGGKVCMIVWRTIDDNPC
                     WGLGKQIALKHLPPPGENAATCGPGPFFLANEDTDREILKAAGFDNVVLFKRNDAEVC
                     VGTSLEEAVDFQILVGPSGEIIREAGELGKEKLPEVRKDMEDEMRKTLREDGVYLPSS
                     TWFIMAKK"
     gene            5273..5632
                     /locus_tag="AC159U_00505"
     CDS             5273..5632
                     /locus_tag="AC159U_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HIR3335569.1"
                     /translation="MTMCEDILSRLSKSKFRRSFRLKGKELEYYKAKGHDEIFNHARD
                     FIESRLAPADIPNDGKQTPFRNHPVFVAQHATATCCRGCLEKWHGIKKGKELCEEEIE
                     YVVNMIGKWLENQNENN"
     gene            5705..6259
                     /locus_tag="AC159U_00510"
     CDS             5705..6259
                     /locus_tag="AC159U_00510"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3335570.1"
                     /translation="MALTPSNMIPLGTTAPDFTLPDAVSGNDMNLEQLKSDKATVIMF
                     ICNHCPYVKHVQKGLVELANDYISKGVSFIAINSNDVDNYPDDSPENMKKDAERFGYP
                     FPYLFDESQQIAKAYDASCTPDFYIFDAELKLVYRGQMDDSRPGNGRPVTGKDIRKAL
                     DQILEGQSLSPDQIPSIGCNIKWK"
     gene            6420..7202
                     /locus_tag="AC159U_00515"
     CDS             6420..7202
                     /locus_tag="AC159U_00515"
                     /inference="COORDINATES: protein motif:HMM:NF040196.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HIR3335571.1"
                     /translation="MKKFMIALPILFIICMPNISYGVGGISNSKVIVPSADTVPKNRF
                     EVEPFFSFTAIDDAADSRNYGFGSRFTYGFLDNLEAGFNLNFLEIEDSDVQDSNSDFG
                     NIEAGVKYRLIDQTDNFPFSLAYQGGVTFPTAGDDEPWVFEPVGLILTRNYTDKLSSD
                     LDVVFSFDEDDNVAVVSNLGVGYFVSPLLQPVVEVSYTYEDPDSGSSTEVFNGTVGFT
                     SPVNEMLTIIMGVTYDLYTDNTDEAFTVSAAFTVSAAFTFLF"
     gene            7286..7693
                     /locus_tag="AC159U_00520"
     CDS             7286..7693
                     /locus_tag="AC159U_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR3335572.1"
                     /translation="MEITPTVEDYLKTIFYLQKDSEVVSTSSLAERLNIKDASATAMI
                     KKLSENKFINYVSHKGVNLTDSGKEIAINIIRKHRILEIYLNEKLNVSTDKVHGEAER
                     LEHYVSEDVMKKIENIVGQREFGIHGEKIPVLN"
BASE COUNT         2428 a         1597 c         1430 g         2298 t
ORIGIN      
        1 atgttaaatc aggtgccaat agtctcagta aacttaaaat ttgattcaag aatatttatt
       61 atttcttcat tttttgcata caccgcccaa taggctgcat ccacgcacct gaaaaacacg
      121 actgcatttt cttcgatact atcagagtct gtggggtcaa acgtcttcaa cttttgtata
      181 tcatcataac cggcaagctc tacccactca gaaaaaatgt attgcccaaa attctcaata
      241 aaccctctgt gcatatagtt tattgatctg gccttatctt catgaagaag atattcttct
      301 aaattccttt ttgtgatatt gtactgttcc tgtgtaagac tttccggaaa actgtagatg
      361 atacctgtat gagcccagta tagctctgaa acaagctctt ctatattgtg taatatttct
      421 gcaaatgaga aatccagggt agaaaattgt gacgatgccc cggattcaga catcctttcc
      481 tttcctaaaa tccttatggc cttcattttg atatcccgac cttaatttga ttcacatatg
      541 ttctgaaata tttctaactg atcatgactc ccgatcacgg ccagcctgtc cccggcttta
      601 aaaatatatt cggagccggg atttggatta actccaccac tgccaagtat acctactatt
      661 gaaacgcccg tcttccttct tatatcaagt tccctaatcg ttttacctaa tagcgggctg
      721 tcatctgcta gattgatcca gcttagatca agcatatttg cagccttctt aagatcggcc
      781 atctctttat attccttgtt tatcctgtaa agaggctcat agaggtcttg tcttacggca
      841 tcctcatatt ttgtgatttc ggcagcggga acattcaggt gaagcagcgc ctgtcttgtt
      901 atttccagcc cggcctcaaa gtgtggctgt accacttcat acacacccat atcgtgtagt
      961 gtttttatat gctctatgtc ttctgttctt gccactatat gaagattcgg attaatagat
     1021 tgaacggtgc taactacaga ttgcgttatc agaattgatg gaattgtgac aataagcagg
     1081 cgtgcgtcat ttattttagc agcatcgagc actactcctt gtctgatgtt tccgaaaatt
     1141 acgggcatat cggcattttt tgcgtccaat actctgttgt agttaaattc gataatcaca
     1201 aaatcaagct ttaatagttt taatattttt gagatatgtt tacctactct gccgtaacca
     1261 gctattacta tatggttatg aagtcctgac tttgggatat tcatggtatc catctcttca
     1321 taatgcagcc ttcccttgaa aaaggtataa atcgggccag taagacccga gataagcggc
     1381 gtcaatacca tcgttattac agcagtgttt aatatgagag aataaagctc ggtgctgatt
     1441 gagcctgagc ttagaccgag tcttgcaagt acgaaagaaa actcacctac ctgaaacagt
     1501 ccaagaccaa gagcaaaagg tattacgttt ctgtaattaa acagaaggct tattccacta
     1561 aagattaaac ccttcccgat cattacaagc actactaata gcaatattgt ctttatattt
     1621 gagattaaaa atgaaggatc aagcagcata cccacagata caaaaaacag gaggccaaaa
     1681 atatccctta gtggaactat atcgcttagg gcctgatgac cataatcaga ttcgctcagc
     1741 acaattcctg ctataaatgc cccaaatgca aatgaaagcc ctaccagaaa tgaagcatat
     1801 ccaacaccaa gacctattgc tgttatggct ataagaaaca actctcttga gttccagcgc
     1861 gctatgtgtg ataaaagaac cggcaggatt ttagttccaa ctaatatcat gactcctaaa
     1921 aaggtaaacc ccttaagggc agctatacca atcaaggata tcccagaccc aaggtttgct
     1981 agttgaggta gaattatcat catgggaata actgcaagat cctgcacaat gagcatgcct
     2041 atcataacac ggcttgatag tgtacccatc ctcccctggc tcattagtgt tctcaaaata
     2101 accatggtac tggaaattga aatcagcgcg ccaaaccata tggaagagtt ccagtccaga
     2161 tcaaatagtc ttcctatgcc tgcaccaaat gcagttgtaa gaattatctg gataggtgtt
     2221 ccgaagagtg caatgttacg caccgggttt aattctttta gagaaaattc gagccccaag
     2281 gcaaaaagca gcaatgccac accaatctct gcaagaagct caatattgtg aacctcagat
     2341 acttctataa aacctgtata gggacctacc gctactcctg caaggataaa cccaataata
     2401 agcggctgcc taagcatgcg cgcggcagaa cctccgataa gcgctgctac gataatgata
     2461 gctatatcag ctgcaattcc catagctatc tattttacct gtctatcgaa caaaaattag
     2521 attcataaga aatttgtaaa gatagtggac tgttactgaa taatatgatc ttgcttagta
     2581 catcacttat gatacacttt ctgtatgcca acaaaaaacc cccgaattaa cattgtatta
     2641 gaaaagccgc tttataatgc gattaagaag atcgcaaaaa aggaaggtat atccatctca
     2701 ttaaaggcca gggattttat tcgagaagca ttagaaactc acgaagatgc cctgctggat
     2761 gaaattgcat cagaaagaga aaagggcttt tcaaaagaaa aagctctctc tcatgaagat
     2821 atttggtctt aatacaatca tgccatggca tactctataa tttatcatcc agatgttaaa
     2881 gatgacatta agaaatttga cagtaagata aaacaaagag tacaagatgc aattgaacaa
     2941 agactctcta ctgagccaga aaaatatgga aaaccactta gaaagtcact aaaaggattc
     3001 tggaagctaa gagttggaga cataagaatt gtgtttaaga ttctaaaaag cgaaatagta
     3061 gttctagcaa tcttgaatag aaaagaagtt tatgaacgga tcaacaaacg aatagagtaa
     3121 taattattat tagatctcat cctcagcaat ccagtgctgg acctgagggg gtttgtattg
     3181 ttcgaatttt cctaacagtg ctgaggggtc cgtttcgaca atgagcatcc ttctgtatat
     3241 atccataaaa aacttttctt ctgatatatg gtttaaaaat tcgatgagct tgtcataaaa
     3301 cccgagcaca ttgattatgc cacagggttt gctgtgaaac ccaagctgac tccatgtgag
     3361 catttcgaag gtttcttcaa ttgttcctat accacccgga agcgaaataa acccattaga
     3421 tagctgtgcc atcttggctt ttctctcatg catcgacccc accactataa gatcagtaat
     3481 tgtatcatga gccacctctt ttaaggcaag tgcttcagga ataattccaa taactttacc
     3541 accaagctca attgctctct cggcaatcac tcccataagc ccgattctgc caccgccata
     3601 aacaatctct atatcatgtg aaacaagctc ctcggcaagt tttcttgccg cttcagcata
     3661 ctcaggttta ttgcctttat ttgctccgca gaatacacat attcttttca tttttcgttc
     3721 cattgtattt aattgggcag aagataactg gcacgttaat aatattcaat caagtttagt
     3781 aaagttagtt tagcgaatta cttagattat tcagatttat cttccagtcc cagacgctcg
     3841 tgccagccgg cgattgattc gtccgggtat tcgggatgct ctttatctgc tgagcctttt
     3901 tgaacttcaa cccagccggc tttatattca agcattatat gggttttttc aggaggcgta
     3961 ggaagctctg tatctattgc acttgcaaat ggatgcacct gctccggcca ccttgggtcc
     4021 cagacaaaga gcgagcttcc acattttctg caaaaacttc tttctgcagg gctcttcttg
     4081 tcttcctttg catctttatc attcaggtaa gctcggtaaa cagtgatatt ttccttaccc
     4141 tcaactttta gtgtgctgta atcgccgttg agatttattg catacccgcc tccgcctgca
     4201 gttttcctgc aaatagagca gtagcaatac atatacggat acggatgctg actttctaca
     4261 ctgaatttaa cagctttgca gtggcatgaa ccttctaatt tcaatatgac tcccggacta
     4321 atttattttt tagccatgat aaaccatgta ctcgatggaa gataaactcc gtcttccctg
     4381 agtgtctttc tcatttcatc ttccatgtcc ttcctcacct ctggaagctt ctcttttcca
     4441 agctcaccgg cttctcttat aatttctcct gaggggccta ctagtatctg aaagtcaacg
     4501 gcttcttcaa ggctggtacc aacacacacc tcggcatcat ttcttttgaa cagcacaaca
     4561 ttatcaaatc cggctgcctt tagaatttct ctgtcagtgt cttcattagc caggaaaaac
     4621 gggcccggac cgcatgttgc cgcattttct cccggaggag gcaggtgctt tagagctatc
     4681 tgttttccaa gcccccagca agggttgtca tctatagtac gccatacgat catacatacc
     4741 tttccaccag gtttaagagt cttatgagca ttcttaaggg cataaacaac attttgaaaa
     4801 aacatcacac caaagcgtga atatactacg tcatagtagt tttcgggcag atcatatgtc
     4861 tgcgcatcgc caagctcaaa cctcacgttt gtaagcccgg cctcatctct ttctttgttg
     4921 gcaatatcca aaaaagcctg tgtacagtcc agcccgagca cttcaccctc cgggcctact
     4981 atgcgtccta tatccaggca tgtctcgcca taaccacatc caatatcaag aactttgtca
     5041 ccgggctgaa tatcaaagtc ggcaaaagcc atatcgctgt gcactccgcc gtttcctgaa
     5101 agtatgtggc ggaatctgtt ccattttgga acaagtatct cgttccaaca gtttataaaa
     5161 tcattgtttg catccatatt atttctctcc catagatact gctaagatat attaattata
     5221 atactattaa taaaaagtta tagtcttttt agtaactaat acattatatt taattacaat
     5281 gtgtgaagat attctttcca gacttagcaa atcaaaattc aggaggagtt tcagactcaa
     5341 aggaaaggag cttgagtatt ataaagccaa gggacacgat gaaatattta atcatgcaag
     5401 ggattttatt gaaagcagac ttgcacccgc ggatatccca aatgacggca agcagactcc
     5461 tttcagaaat cacccagtgt ttgttgccca gcatgctaca gctacctgct gtagaggatg
     5521 tcttgagaag tggcatggga ttaaaaaagg gaaagaatta tgcgaggaag aaattgaata
     5581 tgtagttaat atgattggaa agtggttgga aaatcaaaat gaaaataatt aatttattta
     5641 tctgtatctt aataataaat ctgttaaatt aaatacttca aattttaaat atacggagaa
     5701 tattatggca ctaacaccct caaatatgat cccactggga actacagcac ctgattttac
     5761 acttcctgat gcagtgtcag gaaatgatat gaatctggaa cagttaaagt cagacaaggc
     5821 cactgtaata atgtttatat gcaaccactg cccgtatgta aagcatgtgc agaaggggct
     5881 tgtggagctt gcaaatgact atatatcaaa aggtgtgtct tttattgcta ttaattcaaa
     5941 tgatgtagat aactatcctg atgactctcc ggaaaacatg aaaaaagatg ctgaaagatt
     6001 tggatatccc tttccatatc tttttgatga atcccagcag attgcaaagg cctatgatgc
     6061 ttcctgcacg cctgatttct atatattcga tgccgagctt aaacttgtat accgtggaca
     6121 aatggatgat tcacgaccgg gaaatggcag acccgttaca ggtaaagaca tcagaaaggc
     6181 tctggatcaa atactcgaag gacaatcatt atctcccgat cagataccga gtataggctg
     6241 taatataaag tggaaataac ccgtattact acccactaac agctaattat actttccagt
     6301 attagttaaa aattatacca tccaacaaat ctaaattaga gctaatttcc tattgcctaa
     6361 tcaatatttt agtgcatact aaatatttta ttagtcaata caacaacaag gagattaaaa
     6421 tgaaaaagtt tatgatagca ctaccgattc tatttataat ctgtatgccc aacatttcat
     6481 atggagtggg aggtatatca aacagcaagg ttattgttcc tagcgcagac acagtaccca
     6541 aaaacagatt tgaggttgaa ccatttttta gttttacagc catagatgat gcagccgact
     6601 caagaaacta tggatttggc tcaaggttta cttatgggtt tttggataac ctggaagcgg
     6661 ggtttaattt aaatttcctg gagattgagg attctgacgt tcaggacagt aactctgatt
     6721 ttggaaatat agaagcaggg gtaaagtaca ggttaatcga tcaaacagat aattttccat
     6781 tttcacttgc ctatcaggga ggtgttacat ttcccacagc tggagatgat gagccctggg
     6841 tgtttgagcc tgtagggctt atccttacaa gaaactatac agataaatta tcatccgatc
     6901 tggatgttgt tttcagcttc gatgaagatg acaatgtagc tgtagtttcc aatctgggcg
     6961 tgggatattt tgtatctccc ctacttcagc ccgttgtaga ggtttcatat acatatgaag
     7021 atcctgacag cggcagcagc actgaggttt ttaatggaac tgtggggttt acctcacccg
     7081 ttaatgagat gcttacgata attatgggtg taacctatga tttatacaca gataatactg
     7141 acgaagcgtt tacagtctca gcagcgttta cagtctcagc agcgtttacc tttttattct
     7201 agcaaaaagc atttgacaaa ggtctaaaaa agattaaatt tagactatac taaaaatata
     7261 tatttattga tctaaaataa ttattatgga aattacaccg actgttgaag attatctaaa
     7321 aaccattttc tatttacaaa aagatagtga ggtggtttct acctcttcac ttgcagagag
     7381 actcaatata aaagatgctt cggctacagc aatgataaag aagctatccg aaaacaaatt
     7441 tataaactat gtgtctcata aaggggtaaa tctaacagat tcaggaaaag agatcgcaat
     7501 taacataata agaaagcaca ggattttgga aatatatttg aatgaaaaac ttaatgtctc
     7561 aacggataag gtacacggcg aagctgaaag gctcgagcat tatgtgtctg aggacgttat
     7621 gaaaaaaatc gaaaatatag taggccagag ggaatttgga atacatgggg aaaaaatccc
     7681 cgtattaaat taatttaagg aaaattaagt acaactaaaa aaaagatgag cacaaaaaaa
     7741 acattaatta taa
//
LOCUS       DBNGSV010000009         4735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_555137, whole genome shotgun sequence.
ACCESSION   DBNGSV010000009 DBNGSV010000000
VERSION     DBNGSV010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_555137"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="AC159U_00525"
     CDS             complement(<1..2074)
                     /locus_tag="AC159U_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3335573.1"
                     /translation="MKFDKLTIKAQEALSEAQSLAQDQSNQVIDIPHVLFVLLSEQGI
                     PGEIISKLGASSNEIKTIAENDYKALPKVEGAGDQIYVSKELSQALRTAEKEAKSLGD
                     EYISTEHLLIGIVQHSIGNLKNAFKSADITRDNILKALKDVRGSDPVTSQNPEDTMQS
                     LKQFGKDFTELARQGKIDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGDVPEGLKNKKLIALDIGSIIAGAKFRGQFEERLKAVLKEVTDSEGEIILF
                     IDEIHTVVGAGAAEGAMDASNMLKPALARGELHCIGATTLDEFRKHIEKDAALERRFQ
                     QVFVGEPSVEEAISILRGLKEKYEVHHGVKIKDDALIAAGVLSNRYITARFLPDKAVD
                     LIDEAAARLKMEIDSMPTEIDELNRKIMQLEIEKQALSKEKDASSKDKLKNIEKDIAN
                     LNEEVSGLKAHWQREKDTITEIRKTKEEIDNVKVEAERAQRIGELNQASELLYGKLPD
                     LHERLEKLNTELVEIQKSKKMLKEEVDAEDIAQIVAKWTGIPVSRLLEGEVEKLIQME
                     ERLVKRVIGQDDAIKAVSDALRRSRSGLSDPNRPIGSFIFLGPTGVGKTELAKALAEF
                     MFDDESALVRIDMSEYMEKHTVSRLIGAPPGYVGYEEGGQLSEAVRRRPYTVILFDEI
                     EKAHSDVFN"
     gene            2279..2650
                     /gene="speD"
                     /locus_tag="AC159U_00530"
     CDS             2279..2650
                     /gene="speD"
                     /locus_tag="AC159U_00530"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HIR3335574.1"
                     /translation="MESLGQHYLIELYECDPHILDDLDAVKEIMLSAAKKSGATILKD
                     FLHKFSPQGVSGVVIIAESHLAIHTWPENNYAGVDFFTCGAKVDPLVAYAYIKEKLHS
                     RHSNYTILERGKIEELEIAAG"
     gene            complement(2666..2998)
                     /gene="trxA"
                     /locus_tag="AC159U_00535"
     CDS             complement(2666..2998)
                     /gene="trxA"
                     /locus_tag="AC159U_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3335575.1"
                     /translation="MASGNVNEFSDDNFESEVINSDLPVLVDFWAPWCGPCRIIAPVV
                     EELSESLAGKLKVGKLNVDDNQQVSMKFGIRSIPTLLVFKDGEVAEQIIGAVPKTEIE
                     RVVAKVME"
     gene            complement(3135..4412)
                     /locus_tag="AC159U_00540"
     CDS             complement(3135..4412)
                     /locus_tag="AC159U_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3335576.1"
                     /translation="MSTELTGSNKKTVLTSSISIIIEWYDFSLFGFLAVVLAKLYFPS
                     ENETLSIIATYSVFAVSFIFRPLGGLILGRIGDSVGRKNVLLLSIIFITVPTVLIGLL
                     PTYEQIGILATILLVIFRIAQGFSIGGERSASLSFLVENAPENLRGVFGSVSLFSTCM
                     GIVLASAVISFISSILSPEQFLSWGWRIPFLLGIANGIVAYYLKKNVSESETFTKLKE
                     SGNIAKAPINEALQKNWKELSLILGITIAIAVPFYIIFIYIIRHAVAFESMHISQILK
                     INTIDLFIITLFIPVFGYISDRVGRKPVLIIGCLSSVITSYLFFPLFSNDALMYKILI
                     QICAGIAMSTVAGAAAPYMVESLPARVRMSGLSLGHVIGFSIFGGSAPLVATYMIKQT
                     GDIGSPGIYLTFCCVISLTAALLIKETYKGKIS"
     gene            4499..>4735
                     /locus_tag="AC159U_00545"
     CDS             4499..>4735
                     /locus_tag="AC159U_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335577.1"
                     /translation="MNIAKLTIIVLVLILTTASAFSQAPPPTPVEVALVEEKVLNRPV
                     SFVGNVVPFKRSVIASEVQGLVSNFPAYEGLFVKE"
BASE COUNT         1326 a          996 c          860 g         1553 t
ORIGIN      
        1 cgttaaatac atcgctatga gctttttcaa tctcatcaaa gagtatcacg gtataaggtc
       61 ttctccttac ggcctcagag agttggccgc cttcttcata accaacatat ccgggagggg
      121 cgccaatgag ccttgaaacc gtgtgctttt ccatgtattc actcatatcg attcttacca
      181 gtgcactctc atcatcaaac ataaactcgg caagagcctt tgcaagctca gttttcccaa
      241 cgcctgttgg ccccagaaat ataaatgaac ctataggcct gtttggatcg gaaagcccag
      301 agcgtgagcg cctgagcgca tcggaaactg cttttattgc gtcgtcctga ccgattaccc
      361 tttttacaag cctctcttcc atctgtatca gtttttctac ttcaccttca agcagacggg
      421 atacgggaat gccagtccat tttgcaacta tctgtgcaat gtcctcagca tcaacttctt
      481 cttttagcat ttttttgctc ttttgtatct caaccagttc tgtatttagc ttttctaatc
      541 tttcatgcag gtctggaagt tttccatata acaattcaga agcctgattt agctctccga
      601 ttctttgagc tctttcagcc tcgactttta cattatcaat ttcttctttg gtttttctaa
      661 tttctgttat tgtgtctttt tctctctgcc agtgtgcctt gagacccgaa acctcttcat
      721 tcaggtttgc aatatccttt tctatatttt tgagcttatc ttttgaagaa gcatcctttt
      781 cttttgacag tgcctgtttt tcaatctcta gctgcattat ttttctgtta agctcatcta
      841 tctccgtagg catggaatct atttccatct tgagccttgc agctgcttca tctatcagat
      901 ctactgcctt atccggcaga aaccttgctg tgatatatct gttggacagc acacctgcag
      961 caatgagcgc atcgtctttt atttttactc catgatgcac ttcgtatttc tcttttagtc
     1021 ccctaagaat agaaattgct tcttctacag aaggctctcc aacaaacacc tgctgaaacc
     1081 ttctttcaag tgcagcatct ttttcaatat gttttctgaa ttcgtcaaga gtagtagcac
     1141 caatacagtg aagctcccct cttgctagtg caggttttag catatttgat gcatccatcg
     1201 ccccctctgc tgcaccggca ccgacaactg tgtgaatctc atctatgaac agaattattt
     1261 caccctcaga gtccgtaact tctttaagta cagcttttaa tctttcctca aactgtcccc
     1321 taaacttggc tccggcgata atggagccaa tatccagtgc aatgagtttt ttattcttta
     1381 gcccttcggg tacgtcaccg tttacaatcc tctgggcaag tccctctaca atagcagttt
     1441 ttcccacacc cggttcacca ataagtacgg ggttgttctt agttcttctg gaaagtacct
     1501 gaatcaccct tctgatttct tcgtcccttc caattaccgg gtcaatcttt ccctgtctgg
     1561 caagttcggt gaagtctttt ccaaactgct tcaaagactg cattgtgtct tcggggtttt
     1621 ggcttgtaac agggtctgaa ccccttacat ctttaagtgc ttttaagata ttgtccctgg
     1681 ttatatcagc gcttttaaac gcatttttca gatttcctat agaatgttga acaattccta
     1741 taagcaggtg ttcggtgctt atgtattcat cacctaaaga ctttgcctct ttttctgctg
     1801 ttcttagagc ctggctaagc tctttgctta cataaatttg gtctccagcc ccctctacct
     1861 tgggtagagc tttatagtca ttttctgcta tagttttgat ttcatttgat gacgctccga
     1921 gcttggaaat aatctcgcct ggaattcctt gctcagagag aaggacgaac aaaacatgtg
     1981 gtatgtcgat tacctgattg ctttgatctt gcgctaaaga ctgtgcttca ctaagcgcct
     2041 cctgtgcttt gattgtaagt ttgtcaaatt tcatatttat ctccctaatt aatatctata
     2101 aagataaaat attaatcctt tttgttattt caaggtctga atcgggataa atttttcaac
     2161 taagatacaa ttataagaca tccatattta aaggatatga ctttgactaa tgataaatta
     2221 attgtatatt ctcgccttac tttttttata aaaagagtaa tatttcaaag aggtgaaatt
     2281 ggaaagttta gggcagcact atctaataga attgtacgaa tgtgatcctc atattctaga
     2341 tgatctggac gcagtaaaag agatcatgct ctcagctgct aaaaaatccg gagcaactat
     2401 ccttaaagat ttccttcata aattttctcc acagggagta agcggggtag taattattgc
     2461 agagtctcat ctggccatac acacctggcc tgagaacaat tatgcagggg ttgatttctt
     2521 tacttgcggg gcaaaagtag atcctctggt agcttacgca tatataaagg aaaagctgca
     2581 ttccaggcac agcaattata ctatacttga gaggggaaaa atcgaagaac tggaaatcgc
     2641 tgcaggatag ccggctatcc tgcttttatt ccataacttt tgctacaact ctttcaatct
     2701 ctgtttttgg aactgcacct ataatttgct cggccacttc tccatcttta aaaacaagca
     2761 gggtgggaat acttctaata ccaaacttca tacttacctg ctgattgtcg tcaacattta
     2821 atttgcccac ttttagtttt cctgcaagag attcagatag ttcttctact accggagcaa
     2881 taattctgca tgggccgcac cagggtgccc aaaagtctac taataccgga agatcgctgt
     2941 tgattacctc gctttcaaaa ttatcatcgc tgaattcgtt tacgttaccg cttgccattt
     3001 acttctcctt tttttcttaa gttaagcatc tagtatgcta tctaatatta gattttcaag
     3061 atgatttaaa tttttgatta cttttataat gcctataatt gttaagatag tattaagcag
     3121 atttggattc tacattaact gattttgcct ttgtaggttt ctttaataag aagtgcagca
     3181 gttagtgata ttacacagca aaatgtaaga taaattccag gtgatccaat gtctccggtt
     3241 tgttttatca tatatgttgc cacaagaggt gcgctacctc caaagatgga aaaaccgatc
     3301 acatgtccaa gagatagtcc ggacatccta acccttgcgg gcagggattc taccatataa
     3361 ggcgctgccg caccggcaac tgtagacata gcgataccgg cgcaaatctg gatcaatatt
     3421 ttgtacatga gggcatcatt agaaaatagc gggaaaaaca gataagaagt aattacagaa
     3481 gataaacagc ctataataag taccggtttt ctgcccactc tgtcagatat atatccaaat
     3541 acaggaataa aaagagttat gataaatagg tctatagtgt ttatttttag tatctgggaa
     3601 atatgcatgc tttcaaatgc caccgcatgt cttattatat agataaaaat aatgtaaaaa
     3661 ggaacagcaa ttgctattgt aattccaagg attagagata gctctttcca gtttttctgc
     3721 agagcttcat ttatgggtgc cttggcaata ttcccggatt ctttaagttt agtgaatgtt
     3781 tctgattcgc tgacattttt tttgagataa taggcgacta ttccatttgc aattccaaga
     3841 aggaagggga ttctccatcc ccaagagagg aattgctcag gggataatat tgagctgata
     3901 aaggaaatta cagctgaagc aagtacaata cccatacagg tactaaaaag tgagacactt
     3961 ccaaaaaccc ctctcaggtt ttccggagca ttttctacca gaaatgaaag agatgcagat
     4021 ctctcgccgc ctatggaaaa tccctgagct attctaaata ttactagaag aattgtggct
     4081 aatataccta tttgctcata agttggcaga agcccaatca gaacagttgg aaccgtgata
     4141 aatattatag aaagcagcag tacgtttttt cttccaacac tgtctccaat tcttcctaat
     4201 attaaaccgc caagtggcct gaaaataaaa ctcacggcaa atacggagta ggttgcaatt
     4261 atggatagag tctcattttc ggatggaaaa taaagcttag ctaatactac ggctaaaaat
     4321 ccgaatagtg aaaaatcata ccattcaata attatagaaa tagaactcgt taatactgtt
     4381 ttcttgtttg aaccggttag ttctgtagac attaacttaa tttttaaaat tcatgtggca
     4441 attcgataat attaagtcaa tatatataaa ttaaaaggga ttaaatttaa aaacgtttat
     4501 gaatattgct aaattaacta taattgtgct ggtattaata cttactacag caagcgcatt
     4561 ttctcaggcc ccgccgccaa cacctgtaga ggttgcactt gtagaagaaa aagtactcaa
     4621 cagacctgtg agttttgtgg gtaatgtggt gccgttcaaa agaagcgtaa tagcaagcga
     4681 ggtacagggt cttgtaagca acttcccggc ttatgagggg ctttttgtta aagag
//
LOCUS       DBNGSV010000010         7195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1593968, whole genome shotgun sequence.
ACCESSION   DBNGSV010000010 DBNGSV010000000
VERSION     DBNGSV010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1593968"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="AC159U_00550"
     CDS             <1..910
                     /locus_tag="AC159U_00550"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3335578.1"
                     /translation="TDNKVRVVLDINSFEKYNVFHLQNPFRIVMDIYGDKKSSPTTAI
                     AHSHTQSDIKSNSNRNTESLAGILGLKIRRVVIDPGHGGHDPGAIGPSGLKEKDVNLK
                     IAKALKAKLQKDGKKYGIEHVSLTRDNDKFIRLEDRTTIAQKKDADLFISIHCNAAKS
                     KNAYGIETYILSLTKDKRALEVAARENALSTQDISDMENIIKKIVNNAKIDESKELAS
                     HVQNSIITKVTKKYSNVKNKGVKKAPFMVLIGADVPSILVESSFITNPREEKRLKNNN
                     YIDKIADGIFAGVVEYSKGMQTAYAK"
     gene            911..2638
                     /gene="polX"
                     /locus_tag="AC159U_00555"
     CDS             911..2638
                     /gene="polX"
                     /locus_tag="AC159U_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3335579.1"
                     /translation="MSKNADIANIFSRIASSLQILDENRFKIRAYQNASRNISELSED
                     LEKLAKSGSLSSIPGVGKDLALKIEEYLSTGKIEYYEKIQKEVPSSLVDLLRIQSMGP
                     KTLSLLYKEFGVKNLTDLEKVLKTEELEKIKGMGKKKIEDLHKGIELMKKGLERLNIG
                     IAFPIAQSIVNLIENIQGTKKTTFAGSLRRMKETIGDIDILTSTEDGEKVIDEFVKLP
                     FVTEVLASGDTKGSVIIENDVQVDLRVVGEDSYGAALLYFTGSKEHNVKLRTMAVKRG
                     LSINEYGIYEGDKKLAGRTEKELYEVLGLPLFPPEIREDRGEFDAAASKSLPNLISFQ
                     DIRGDIHCHSNWSDGKSTIEEMARAAKKMGYEYIAITDHSPASRIANGLDIKRLKQKF
                     KEIEEVRKKVKDIKILSGTEVDILSDGSLDYPDKILKDLDVVVASVHSGFKMEKSAMT
                     KRIIRAIKNPYVHILGHPTGRLINEREPYNVDLEEVFKITKQYGKAVELNASYMRLDL
                     NDIHSRRAKDLGIPISINTDAHHTDQLQFMLYGIGTARRGWVEKNDVINCMSYSKLTK
                     WLKQVRDHT"
     gene            2656..4671
                     /gene="ligA"
                     /locus_tag="AC159U_00560"
     CDS             2656..4671
                     /gene="ligA"
                     /locus_tag="AC159U_00560"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3335580.1"
                     /translation="MSSIPFKDAKEKANKLRQEISLHDRLYYIENQPSISDSEYDALM
                     RELRSIEEEHPELITPDSPTRRVSGTVADGFDNIDHIVPMISIDNIQNEDDAYDFDKR
                     VKKYLELDENIDIEYVCEPKFDGVSASLTYENGILTKGATRGDGRKGEDITQNLKTIK
                     AIPLSLNTNNKQPKLIEIRGEVIYANEDFAKLNKQLAAEGKPVYANARNTASGSLRQL
                     DPSITASRPLDFYGWGIGHSEGLNGTTEWEVIENIKKWGFKIDARIAKFDNISGAISY
                     HHEIENSRDDLPYDADGVVIKVNDKKYQRELGSTAKYPRWAVAYKFKPRQATTVVNDI
                     IVQVGRMGLITPLAKVQPVVIAGVTISNASLHTEDIVMQKDVRKGDTVLIERAGDVIP
                     QIVKSIPEKRTGKEKKFKMPSNCPSCKTPIEKEGAYYYCPNLSCPDQIKGRIEHMASR
                     RAFDIVGLGEKIVVQFLEQGVIKDPSDIFYLKKENIINLERFADKSTENLINEIEKNK
                     KIPFDRFLNAISIRHVGQRMSQVIAENFGDLDSLMNASVQELEQIDSVGPEIAKSIVN
                     FFSIESNVNSINKMLGGGVEIIYSQKAKGDKLAGKTFVLTGTLENYTRDEAKEIIENE
                     GGKVTSSVTKRTSYVIAGESPGSKYDKAMKLDIPIIDENEFKVLIGK"
     gene            4719..5531
                     /locus_tag="AC159U_00565"
     CDS             4719..5531
                     /locus_tag="AC159U_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3335581.1"
                     /translation="MVQYFIELNPIYQALIATCFTWFLTALGASVVFVTKSINKKLLD
                     FSLGLSGGVMIAASVWSLLIPSIDMSESRYELPWVPAAVGFLFGAAFIRVADKIIPHL
                     HLGFPLAEAEGLKTHWRRNILLVLAITLHNIPEGLAVGVAFGALATGFEGATLASAVT
                     LALGIGIQNMPEGLAISMPLRRDGMSRLKSFYYGQLSAVVEPIAACIGAAAVLYINPI
                     LPYALGFAAGAMIFVVIEELVPESQRSGNTDLATSGTILGFILMMILDIQFG"
     gene            complement(5568..>7195)
                     /locus_tag="AC159U_00570"
     CDS             complement(5568..>7195)
                     /locus_tag="AC159U_00570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3335582.1"
                     /translation="FLISFATYLFVTRAHERHMLMPIVLLSFLIIRYKEYIIYLTVLS
                     GIYVFQMFWAYKRLITDYTTFTHNFFTPLIYFLGCILILVFIFMLIDYLKKSGAVSDL
                     VKFVKHICVKYKNNLISRNIKTVILVSAVIVGFLVRINDVGYPDKFMFDEVYYAFTAQ
                     EITKGNLYAWGKDYSYPGINEFEWTHPPLGKEIISLGILVFGDSPFAWRIFQVLFGAA
                     AAMLIFLLARNIFNSVNIGMFSAILYLLDPFPFVLSRIAMVDIFLFVFLISACLFTVK
                     YLRKENNKFLYLSGIFLGLAVSVKWTALSFNLFILICLFISYLYKNKDKKIEIKPASQ
                     KLLLLTLSLVFIPIVIYILSYIPFFIYKQSFSEFIALHKSMLRYHESVKNPHNYQSAW
                     WSWILVYKPLLLYFEKVGRLREYIYTIGNPVIWWTGIAAIIAAIIQIIRTRSYPLIFV
                     ISGMFISWLPWILSPKKVIFIYHFLPTLMFLYILVAYFIDKIWSISTKGKMIVLLYFF
                     LSLSAFVYLFPILTAETISVKEFNNLLWFKVWK"
BASE COUNT         2614 a         1222 c         1435 g         1924 t
ORIGIN      
        1 tacggacaat aaagtaaggg tagtactgga tataaatagc tttgaaaaat ataatgtatt
       61 tcacctccaa aatcctttca ggatagtgat ggatatatat ggtgataaga aaagctcacc
      121 aactactgca atagctcatt cacatacaca atcggatatt aaatctaact ctaatcgaaa
      181 tactgaaagc ctggctggta ttcttggctt aaaaataaga agagtggtaa tagaccctgg
      241 ccatggtggg catgatccag gcgcaatcgg tccttctggt ttaaaagaaa aggatgttaa
      301 cctaaaaatt gcaaaagctc ttaaagccaa actacaaaaa gacggcaaga aatatggaat
      361 agagcatgta tcattaacaa gagataatga taaattcata agacttgaag acagaacaac
      421 cattgctcag aaaaaagacg ccgatctttt tatatctatt cactgcaatg cagcaaaaag
      481 caagaatgcc tatggtatcg aaacatatat actcagtctc acaaaagaca aacgagctct
      541 tgaagttgca gcaagagaaa atgctctttc aactcaggat ataagcgata tggaaaacat
      601 catcaaaaag attgtgaaca atgccaaaat agacgaatca aaagaacttg catcacatgt
      661 gcaaaactca ataattacaa aagtgaccaa gaaatacagc aatgtgaaaa acaagggagt
      721 aaaaaaagct ccattcatgg ttctaattgg cgctgatgta ccaagcatac ttgttgaatc
      781 ttcttttatt accaatccga gagaagaaaa gaggcttaaa aataataact atatagacaa
      841 aattgctgac ggcatatttg ctggtgtggt agaatattct aaaggaatgc aaacagcata
      901 tgccaaatag atgtcaaaga acgcagatat agcaaacata ttcagccgta ttgcatcatc
      961 gctgcagata ctggatgaaa accgttttaa gattcgtgcc taccagaatg cttcgagaaa
     1021 catatcagag ctttcagaag atttagaaaa gctcgctaaa tcaggttcac tttcttcgat
     1081 accgggagtg ggaaaagacc tggctctaaa aattgaagaa tacctgtcta caggaaagat
     1141 tgagtattat gaaaaaattc aaaaggaagt cccctcctct cttgtagacc ttcttagaat
     1201 ccagagtatg ggtccaaaga cactttcact tctgtataaa gagtttggtg ttaaaaacct
     1261 tactgatctt gagaaagttc ttaaaaccga ggagcttgaa aagattaagg ggatgggcaa
     1321 aaaaaagatc gaggatttac acaagggaat tgagcttatg aaaaaaggcc tggaaaggct
     1381 aaatataggc attgcctttc caattgccca atccatagtg aacttgatcg agaacattca
     1441 aggaacaaaa aagaccacat ttgcgggttc actcagaaga atgaaggaaa caattggtga
     1501 tatagatata cttacaagca cagaggacgg agaaaaagtt atagatgaat ttgtaaagct
     1561 tccttttgtg accgaagttc ttgcatcggg ggataccaaa ggcagtgtaa tcatagaaaa
     1621 cgatgtacag gtagacctga gagtagttgg agaagactct tacggagcag ctctgctgta
     1681 ttttacaggc tctaaggaac ataatgtaaa gcttagaaca atggctgtga aaagagggct
     1741 gagtattaat gaatatggga tttacgaggg tgataaaaaa cttgctggac gtactgaaaa
     1801 agaattatat gaagttctag ggcttcccct gtttccaccc gaaatcaggg aggacagagg
     1861 tgagtttgat gctgcggcaa gcaaatcttt accaaatctt ataagttttc aggatataag
     1921 aggagatata cattgccatt ccaactggag tgatgggaag agtaccattg aggagatggc
     1981 acgagctgca aaaaaaatgg gttatgaata tattgccatt acagatcatt cccctgcttc
     2041 acgaattgct aatgggctgg atataaaaag gcttaaacaa aaatttaaag agatagaaga
     2101 agtaagaaaa aaagtaaaag acataaaaat actttcagga acagaggttg atatactaag
     2161 cgacggttca cttgattatc ccgataaaat actaaaagac ctggatgtgg ttgtagcatc
     2221 tgttcacagc ggctttaaga tggaaaagag tgcaatgaca aaaagaatta taagagctat
     2281 aaaaaacccc tatgtacata ttctgggcca tcctacagga agacttatta atgagagaga
     2341 accctataat gtagacttag aagaagtatt caaaatcact aaacaatatg gcaaagcagt
     2401 tgagcttaat gcatcataca tgaggcttga tctaaacgat atacacagca gaagagctaa
     2461 agacctaggt attccaatta gtataaacac agatgcccat catactgacc agcttcagtt
     2521 tatgttatac ggcataggta ctgccagacg cggatgggtt gagaaaaatg atgttataaa
     2581 ctgcatgagt tacagcaaac taacaaagtg gctcaaacag gtacgggatc atacttagtt
     2641 aacaaggact tttatatgtc atcaataccg tttaaagatg ctaaagaaaa ggctaataaa
     2701 ttaagacagg aaataagcct gcatgacagg ctctactata tagaaaacca gccatctata
     2761 tcagattctg aatatgatgc attaatgaga gaacttagaa gtatagaaga ggaacatccg
     2821 gagctaatca cccccgactc ccctaccagg agagtaagtg gtacggtagc agatggattt
     2881 gataatattg atcatattgt gccaatgata agtatagaca atattcaaaa cgaagacgat
     2941 gcttatgatt ttgacaaaag ggtaaaaaaa tatttggagt tggacgagaa cattgatatt
     3001 gagtatgtgt gtgagccaaa atttgatggg gtatccgcct ctttaacgta tgaaaatgga
     3061 attcttacaa agggcgctac aaggggtgat ggcaggaaag gagaggatat aacccaaaat
     3121 ctaaagacaa ttaaagctat tccacttagt ttaaatacca acaataaaca gccaaaactt
     3181 atagaaataa gaggtgaggt tatttatgca aatgaagatt ttgcaaaact aaataaacaa
     3241 ttggccgcag agggaaagcc ggtatatgca aatgcgagaa atactgcatc cggctcattg
     3301 agacagcttg accctagcat aaccgcctcg agaccacttg atttctatgg ctggggaata
     3361 ggacattcag aaggattaaa tggtacaaca gagtgggaag ttattgaaaa tattaaaaaa
     3421 tggggtttta agatcgatgc tagaatagcc aagtttgaca atatttcagg tgcaatctca
     3481 tatcatcacg agatagaaaa ctccagggac gaccttccct atgatgcaga tggtgttgta
     3541 ataaaagtta acgacaagaa atatcaaaga gaacttggat cgactgctaa atatccaagg
     3601 tgggcagttg catataaatt taaaccacgg caggcaacca cagttgtaaa tgatattatt
     3661 gttcaggttg gaaggatggg gcttattaca ccgcttgcaa aagtgcagcc ggttgtaatt
     3721 gccggagtta ccataagtaa tgcctcactt catactgaag acatcgttat gcaaaaagat
     3781 gttaggaagg gtgatacggt tcttatagaa agagcgggtg atgtgattcc tcaaatagta
     3841 aaatcgatac ctgagaagag aaccggcaaa gaaaagaagt ttaaaatgcc atctaattgc
     3901 ccttcttgta aaacacccat agaaaaggaa ggtgcatact attactgccc caacctttca
     3961 tgtccggatc agattaaggg acgcatagaa catatggcat caagaagagc ctttgatata
     4021 gttggtcttg gtgaaaagat tgtagttcag ttcctagaac aaggagtaat aaaagacccg
     4081 tcagatatat tttatcttaa aaaggaaaat ataattaatc ttgaaaggtt tgctgataag
     4141 tctactgaaa acctgataaa tgaaattgag aaaaataaaa agataccttt cgacagattt
     4201 ttaaacgcta taagtataag gcatgttggc cagaggatgt ctcaggttat agctgaaaat
     4261 tttggagact tagattcact tatgaatgca agtgtacaag agttagagca aatagattca
     4321 gtaggcccgg aaatagctaa aagcatcgta aatttcttta gtattgagag taatgttaat
     4381 tcgataaaca agatgctcgg tggcggcgtt gaaataattt attcacaaaa agcaaagggt
     4441 gataaattag ccggtaaaac ctttgtactc acaggcactt tagaaaacta cactagagac
     4501 gaagcaaagg aaatcataga aaatgaagga ggcaaggtca cttcatcagt cacaaaaagg
     4561 acaagttatg ttattgctgg cgagagccct gggtcaaagt atgataaggc tatgaaactc
     4621 gatattccca tcatagatga gaatgaattt aaagtgctta ttggtaaata gtggattgtt
     4681 taaagaatct ataaccaaaa taaagatatt aatagataat ggtacaatat tttattgagt
     4741 taaatcccat atatcaagca ctcattgcaa cttgcttcac ctggtttctg actgcactag
     4801 gtgcatctgt agtatttgta acaaagagta ttaacaaaaa actattagac ttttctttag
     4861 gtctttcagg cggagtaatg attgctgcaa gcgtctggtc tcttcttatt ccttcaatag
     4921 atatgtcaga atcaaggtat gaactgccgt gggtacccgc agcagttggt tttctttttg
     4981 gcgcagcttt tataagagta gcagataaaa ttattcccca ccttcacctt ggcttcccgc
     5041 ttgcagaagc tgaaggctta aagactcact ggagaagaaa catcttgctt gtccttgcaa
     5101 ttactttgca caatataccc gaaggtctag cagtaggtgt agcatttggc gccctagcca
     5161 cgggatttga aggcgccact cttgcatctg cagtaaccct tgcccttgga ataggaatac
     5221 agaatatgcc tgagggtctg gcaatatcaa tgcctcttag aagggatggt atgtccagac
     5281 ttaaaagctt ttactacgga caactttctg cagtagttga gccgattgca gcatgtatcg
     5341 gagccgctgc ggtgctatac atcaacccca tactccccta cgcactggga tttgccgcag
     5401 gcgctatgat atttgtggtt attgaagaac tggtacctga atctcaacgc tctggaaaca
     5461 ctgatttagc aacatccggc actatactgg gttttatact catgatgatt ctggacatac
     5521 agtttggata agaaatttac tatttcctga gtattttatt aataatacta tttccagacc
     5581 ttaaaccaga gaaggttgtt aaactccttc acactaatag tctctgctgt aagtatcgga
     5641 aatagataaa caaatgctga tagtgaaagg aagaaatata atagtacaat cattttaccc
     5701 ttggttgata tgctccagat cttgtctata aaatatgcaa ctaggatgta taaaaacata
     5761 agagtaggca gaaagtgata tataaatatt actttttttg gtgaaagtat ccacggaagc
     5821 catgaaataa acattcccga tattacaaat attaatggat atgacctggt tctaatgatc
     5881 tgaataattg ctgctattat tgctgcaata ccagtccacc atatgacagg attaccaatc
     5941 gtatatatgt attcacgcag ccttcctacc ttttcaaaat aaagcagtaa aggcttataa
     6001 accagaatcc atgaccacca ggcggactga taattatggg gattttttac actttcatga
     6061 tatcttaaca tcgatttatg aagcgcaata aattcagaaa atgactgctt gtaaataaaa
     6121 aacgggatgt agcttaatat atagataaca ataggtataa atactaaaga taaagtgagt
     6181 agtaaaagtt tttgactagc gggctttatt tctatcttct tatctttatt tttatataaa
     6241 tacgatatga acaaacaaat gagaataaaa agattaaatg aaagagcagt ccattttaca
     6301 gatactgcaa gccctaagaa tatacctgaa agataaagaa atttattatt ttctttcctt
     6361 aaatatttaa cagtaaacag gcaggccgaa attagaaata cgaaaaggaa tatatcgacc
     6421 attgcaattc ggcttagcac aaatgggaaa ggatcaagca gatataatat tgctgaaaac
     6481 ataccaatgt tcacgctatt gaaaatattt cttgcaagaa gaaatatcaa cattgctgca
     6541 gcagcaccaa acaagacctg aaatatccgc caggcaaatg ggctgtcacc gaatacaaga
     6601 attccaagac ttatgatctc ttttccaaga ggcgggtgtg tccattcaaa ctcgtttata
     6661 cctggataac tgtaatcctt gccccaagca tacaaattac cctttgtaat ctcctgtgca
     6721 gtgaatgcat aatagacttc atcaaacata aatttatcag gatatcccac atcatttatt
     6781 ctgactaaaa aacccacgat aacagctgat accaggatga ccgtctttat atttctactt
     6841 ataagattat ttttatattt aacacagata tgtttaacaa atttcactaa atcagatact
     6901 gctccagatt ttttcagata atcaatcagc atgaaaataa acaccaatat gagaatgcaa
     6961 cctagaaaat atatcaatgg agtaaagaaa ttatgtgtaa atgtggtgta atctgtaatt
     7021 agccttttat aagcccagaa catctgaaaa acataaatcc cgcttagaac agtcagataa
     7081 ataatatatt ctttatatct tattattaag aaagaaagca gcactatggg cataagcata
     7141 tgcctttcat gtgcgcgtgt aacaaacaga taggttgcaa atgagatcag aaaca
//
LOCUS       DBNGSV010000011         4941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_348081, whole genome shotgun sequence.
ACCESSION   DBNGSV010000011 DBNGSV010000000
VERSION     DBNGSV010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_348081"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="AC159U_00575"
     CDS             <1..851
                     /locus_tag="AC159U_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR3335583.1"
                     /translation="LSRSRQIVMKRLDPRIYQIIILSSLLVYGVIELDFEVHLARSIL
                     ILSSALITQYLFTRYLGLGKFDPRSPLISGLSLCLLLRSDSIVLIILCTVITISSKYF
                     LRWQGKHIFNPTNIGIVIMLLLFDNTWVSPGQWGNTAFMGFLIACIGGLVVNRAERSD
                     VTYAFMIFFFSLIFGRAIWLGDPLVIPFHQIQNGALLIFTFFMISDPKTTPDTRIGRI
                     LFALIVSLVAYYFQFFLFNTNALFYALASSSLTVPIIDMFIKGKKYEWKPVQKIQILK
                     PKEALS"
     gene            848..2185
                     /locus_tag="AC159U_00580"
     CDS             848..2185
                     /locus_tag="AC159U_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HIR3335584.1"
                     /translation="MNFLVTSLLLLLGVFTVSHNAYSFCGFYVAKADTKMYNKASQVV
                     LVRDGDKTVITMANDFKGDPKEFAIVVPVPTFIEREQINIGDQKIIDHLDAYSSPRLV
                     EYFDRDPCNVYPSAESAVGRFSLKSADNSSFGTEGLGVAVEAKYTVGEYDILILSAKE
                     GSGLEKWLKQNGYKIPQGASEILGSYIKQEMRFFVAKVNLKEQSKLGFTNLRPIQVAF
                     ESPKFMLPIRLGTVNADGPQELFVYVLNRKGRVETTNYRTVKLPTGMDIPVYVKDEFK
                     DFYLAMFDEQVKKEKMKTVFLEYAWDMNWCDPCAADPLSPEELQSLGVFWLNQGDSIS
                     RKSGLARNVFITRLHVRYDAEHFPEDLIFQETGDRTNFQGRYVLRQPWKGDNTCDATI
                     EYHKRVKTRQEQEAKILASLTGWDINDIRKKMKLDEMVVDVNDEKWWKKIWRD"
     gene            complement(2245..2910)
                     /locus_tag="AC159U_00585"
     CDS             complement(2245..2910)
                     /locus_tag="AC159U_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HIR3335585.1"
                     /translation="MNKLINKLVLVAILITLYLIGISTTYAHEDAKDHKDSANKESTT
                     KDMMGMDEETMKKWMEYATPNENHKVLDGLVGKWDYKLKWWQAPGAKPEKSTGRSKIK
                     WTMDGRFLRHKAKGTSMGQPFRGMGIVGFKNETKKYESVWIDNMGTGMARGTGTYDAK
                     TKKLTEEGTFSCPGEGNKKFRSVMTITGPDTFTYEWYMPGPDKKEYRAMVIDYTRKKE
                     EKK"
     gene            3080..3925
                     /locus_tag="AC159U_00590"
     CDS             3080..3925
                     /locus_tag="AC159U_00590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3335586.1"
                     /translation="MNYGIMMFATDFAIHPAKMAKEVEDRGFDSLWFPEHTHIPASRE
                     SEAPGGGDLPEQYWHTHDLFVALTAAATATTKLKIATGICLIIERDPIITAKEVATLD
                     MLSNGRFLFGIGGGWNAEEMANHGTEYKKRWKIMRERILAMKEIWTENEPEFHGEFVN
                     FDKIWSYPKPTQKPHPPILLGGDGPRTFERIVEYCDGWLPILLPSLDLDTKITELKYK
                     AHASGRDPSEISVSCIIPAPLVKDEMISKAKDLEVERVILGVPPGNEDSVLPLLDRLA
                     KSIFL"
     gene            4063..4569
                     /locus_tag="AC159U_00595"
     CDS             4063..4569
                     /locus_tag="AC159U_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335587.1"
                     /translation="MSAVSCDNDSNAEENFTESFSGSFRSGPMNTDTNEDGSPASIAS
                     LSGDSTFGPITIESVNEFMIVDTTGACPQGQLEFSLVRGNFIKRFAETGELLFGTWQT
                     GTSCFDPETTESETNQSGVFSGGTGQFENAGGPVEINFTSVDLATNAEEGFRFGGTTG
                     TGMGMLEQ"
     gene            complement(4585..>4941)
                     /locus_tag="AC159U_00600"
     CDS             complement(4585..>4941)
                     /locus_tag="AC159U_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3335588.1"
                     /translation="SAPELVELQNKYSEKGLVIIGVHHPKSEETKNNDHVKKQAEVFG
                     FKFPIAQDLEWETINSYWLGKKKRSFTSSSILIDKKGIIRFVHDGGQFFKSDYDLEAN
                     AAFEAMEENILKLLAQ"
BASE COUNT         1492 a          961 c          991 g         1497 t
ORIGIN      
        1 acttgtctcg tagtagacaa atagttatga aaagactcga tcctagaata tatcagatca
       61 taatactatc atccttgctt gtttatggtg taattgagct tgattttgaa gttcatctgg
      121 ctcggtcaat tcttatattg tcctctgctc tgattactca gtatttgttc acaagatatt
      181 tagggcttgg gaaattcgat ccaagaagtc ccctaatatc aggactttcc ctctgtctgt
      241 tactcagatc agattcaatt gttctcataa ttctctgcac tgtaataacc atctccagca
      301 aatacttttt aagatggcag ggaaaacaca tctttaaccc tactaatatt ggaatagtta
      361 ttatgctgct gctttttgat aacacatggg tctcaccagg gcagtgggga aatacggcat
      421 ttatgggatt tctcattgca tgtattggcg ggcttgttgt aaaccgcgca gaaagaagtg
      481 atgtgactta tgcatttatg atcttttttt tttcgctaat atttggaagg gcaatatggc
      541 ttggtgaccc tcttgtgatc ccgtttcatc agatacaaaa tggtgcttta ctaatattca
      601 cattctttat gatatccgat ccaaagacta ctccagacac aagaattggg agaatactct
      661 ttgcactgat cgtttcttta gtcgcttact attttcagtt tttcttgttt aacacaaacg
      721 cgcttttcta cgcacttgct agctcgtcat taacagttcc aataatcgat atgtttataa
      781 aaggcaaaaa gtatgaatgg aaacctgtac aaaaaataca gattttaaaa ccaaaggagg
      841 cattatcatg aattttttag taacatctct gctcctttta ttaggggtat tcacagtatc
      901 acataacgct tacagctttt gcggatttta tgtggcaaag gcagacacca agatgtacaa
      961 caaggcttcg caggttgtgc tggtaagaga cggtgacaaa accgtcatta ctatggcaaa
     1021 tgattttaag ggcgacccga aagagtttgc aattgttgtt cccgtaccca cttttataga
     1081 aagggaacag ataaatattg gtgatcagaa aatcatagat catctcgatg cctattcttc
     1141 tccaagactg gtcgaatatt tcgatagaga cccgtgtaat gtttaccctt cagcagagtc
     1201 tgcggttggc agattttctt taaaatcagc tgataactct tcttttggaa ctgaagggct
     1261 gggtgttgct gtagaagcca agtatacggt aggagaatat gatattttaa tcctgtctgc
     1321 aaaagagggt tctggcctgg aaaaatggct aaagcaaaac ggatataaaa ttccacaagg
     1381 tgcatcagag attctgggaa gctacataaa acaggagatg agattctttg ttgctaaagt
     1441 taatttaaaa gaacagtcaa agcttggctt tactaattta agacctatac aggtggcatt
     1501 tgaatcaccc aaatttatgc tgcccataag gcttggaacc gtaaatgccg atggtccgca
     1561 ggagcttttt gtttatgtac ttaatagaaa aggcagggtt gagactacaa attatagaac
     1621 cgtgaagctg ccaacaggta tggatattcc tgtatatgta aaagacgaat ttaaagattt
     1681 ttatctggct atgtttgatg aacaggtgaa aaaagaaaag atgaaaaccg tgtttttaga
     1741 atacgcctgg gacatgaact ggtgcgaccc gtgcgcagcc gacccgcttt caccagagga
     1801 attacagagt ctgggcgtgt tctggcttaa ccaaggtgat tcgatatcca ggaaatccgg
     1861 tcttgcaaga aatgtattta tcacccgcct gcatgttcgt tacgatgcag agcatttccc
     1921 ggaggatttg atctttcagg aaacaggaga caggacaaac tttcaggggc gttatgtttt
     1981 aagacaacca tggaaaggag ataatacatg tgatgcaact atagaatatc ataaaagggt
     2041 aaaaaccaga caagaacagg aagccaaaat tcttgcaagc cttacaggct gggacataaa
     2101 cgacatcagg aaaaaaatga agctggatga aatggtcgta gatgttaatg acgaaaaatg
     2161 gtggaaaaag atttggaggg attaatgtac taaaaatgag gcggcaatta atgccgcctc
     2221 cttatttttt atttatttaa caagttattt cttttcttct ttcttccttg tgtaatcaat
     2281 caccattgcg cggtactctt ttttatcagg tcccggcata taccactcat atgtgaaggt
     2341 gtcaggtcct gtgatagtca tcacactgcg aaacttctta ttaccctcac caggacagct
     2401 gaatgtgcct tcttcagtca atttttttgt tttagcatca taagtgccgg ttcctctagc
     2461 catacccgtg cccatattgt caatccatac agactcatat tttttagtct catttttaaa
     2521 tcctacaata cccatacctc taaaaggctg tcccatagag gtgcccttag ctttatgcct
     2581 taaaaacctg ccatccatag tccatttgat tttgcttctt ccagtggact tctctggctt
     2641 tgcgcccgga gcctgccacc atttaagttt gtagtcccac ttgccaacca aaccgtctaa
     2701 cactttatga ttttcgtttg gcgtggcgta ttccatccac tttttcattg tttcctcatc
     2761 catacccatc atatccttag tcgtagattc tttatttgca gaatccttgt gatcttttgc
     2821 atcttcgtgt gcatatgttg tgcttatacc tattaaatat aaagtaataa gtattgctac
     2881 caacaccaat ttattaatta atttattcaa aacacgacct ccaaaactta ttttattttc
     2941 ataattatag cggattatta taaccaatca tttattttaa acataacttt attctatcct
     3001 gatgaaaaat cgcatgatat tataatcagt actatttagg atgtatttat atacaatctt
     3061 aataaagagg aaatatatta tgaactatgg aataatgatg tttgcaacag actttgcgat
     3121 ccatcctgca aaaatggcaa aagaggtgga ggacagggga tttgattctc tctggtttcc
     3181 tgagcataca cacattcctg caagcaggga aagtgaggca cccggaggcg gagacctgcc
     3241 tgaacagtac tggcacacac atgacctgtt tgtagcgctt accgccgcag caaccgctac
     3301 tactaaacta aagatcgcaa ctggcatatg cctcataata gagcgcgacc ctattattac
     3361 cgcaaaagaa gtagcaactc tggacatgct ttcaaatgga cgatttttat tcggcatcgg
     3421 cggaggctgg aatgctgaag aaatggcaaa ccatggaacg gaatataaaa agagatggaa
     3481 gataatgcgg gagagaatcc ttgccatgaa agagatttgg actgagaatg aaccggaatt
     3541 tcacggagag tttgttaact ttgacaagat ctggagttat cccaaaccaa ctcaaaaacc
     3601 acatcctccc atacttcttg gaggagacgg cccaaggacc tttgaacgaa ttgttgagta
     3661 ttgtgacggc tggcttccga ttctattgcc ttcactagat ctggatacta agatcacaga
     3721 gcttaaatat aaggctcatg cttccgggcg tgacccatct gaaataagtg tgtcttgcat
     3781 aataccagca ccccttgtaa aagatgagat gatttcaaag gcaaaagatt tagaagtgga
     3841 aagagtaata ttaggtgtgc cacctggtaa tgaagactcc gtactcccct tacttgatag
     3901 actagccaag tctatatttt tataatattc tcgagagttg attttatata gcaatcgaat
     3961 ataaaatatg atatttaatt gaataaattt tcattctata atggaggcaa aatgtgtaaa
     4021 aagtatatag ttttttcatt accattgttt ctgtttgttt taatgtcagc tgtaagctgt
     4081 gataatgaca gcaatgcaga agaaaatttt actgagagtt tttcaggatc attccggagt
     4141 ggtccaatga atacagacac aaatgaagat ggctcccctg caagcattgc aagtctttcc
     4201 ggagacagca cattcggacc aatcacaatt gaaagcgtaa acgagttcat gatagtagac
     4261 acaacaggtg catgtccaca aggacaatta gaattttctc tagtccgtgg aaactttatc
     4321 aagcgatttg cagaaaccgg cgagctgcta ttcggcacat ggcagactgg tacaagctgt
     4381 ttcgaccccg agacaacaga atcagaaaca aatcagagcg gagtattctc aggaggcaca
     4441 ggacagttcg aaaatgccgg cggccctgtt gagataaact ttacttcagt tgatcttgca
     4501 acaaatgcag aagagggttt tcgttttggc ggaactacag gcactggtat gggtatgctt
     4561 gagcaataaa ttgtataata atagctattg agctagaagt ttaagaatat tctcttccat
     4621 cgcttcaaat gcagcgtttg cctcaaggtc ataatcgctc ttaaaaaact gtcctccgtc
     4681 gtggacaaac ctgataattc cttttttatc tatgaggata cttgaagagg tgaatgaacg
     4741 tttcttttta ccaagccagt aagagtttat tgtttcccac tccagatcct gggcaatggg
     4801 aaatttgaac ccaaaaacct cagcttgttt ctttacatga tcgttgttct tggtttcctc
     4861 cgatttcgga tgatgaactc caataataac taaacctttt tcagaatatt tgttctgtaa
     4921 ctccacaagt tcgggcgcag a
//
LOCUS       DBNGSV010000012         1210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_902270, whole genome shotgun sequence.
ACCESSION   DBNGSV010000012 DBNGSV010000000
VERSION     DBNGSV010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_902270"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..>1210
                     /locus_tag="AC159U_00605"
     CDS             493..>1210
                     /locus_tag="AC159U_00605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3335589.1"
                     /translation="MSLKDATAYNIQFENGKPIFIDTTSFEKYTEGQPWIAYKQFCQH
                     FLAPLALMSYTDIRLSQLLKTYIDGIPLDLASSLLPTKTKFSFSFLSHIHLHSKAQKK
                     YEKSGDGKDKKSHVSLNGLIGIIDNLKSTINGLKWAPDNTEWADYYNDTNYTDEAFNN
                     KRDLVTQFFNSVPNASMVWDLGANTGEFSRIASQKGVKTIAFDIDPSAVEKNYLTIIE
                     NDEKNLLPLELNLCNPSPGIG"
BASE COUNT          434 a          216 c          207 g          353 t
ORIGIN      
        1 agagtgaaat gggcttacag aaaccataga ggactggcta aacacaagag aattaattac
       61 ttcaaatggt acaccttttt aaagcaaatc tgactcaaat tagcacacat tttttgcaat
      121 tatgccagat tcgaatataa ccgaggataa caaaaaatag attattttac cgaacctagc
      181 tcatttagag atccgagcgc ttttatattt acaaaaaata gtatcactta ccgtcagatt
      241 aataatagtt acaaggatca atacgattta cttctaagct caggacttta tgaaaaactt
      301 tctaaagata gtctcattgt tgaacatgag gaacaggatt taaacattgc cgcaagctca
      361 gaatttgctt acaaagtaat aaagcccgag caaatttcat tcatatctta tccttatgaa
      421 tggagttttt cacagttaaa agacgccgcc cttctaactc ttaagatcca aaaaattgct
      481 atagagcatg gtatgtctct taaggatgca actgcgtata atattcagtt tgaaaatggt
      541 aaacctatat ttatagatac tacctctttt gaaaaataca ccgaaggtca gccatggata
      601 gcatataaac agttttgcca gcactttttg gcacccctgg ctcttatgag ttatacggat
      661 atacgcttaa gccagttatt aaaaacatat attgatggaa tcccccttga tctggcaagc
      721 agtctgctac ccacaaaaac aaaattcagc ttttcttttc tttcacatat tcaccttcat
      781 tctaaagctc agaaaaaata tgaaaaatct ggagatggta aggataagaa aagccatgta
      841 agtttaaatg gattaattgg gattatagat aacctgaaat caacaataaa tggccttaaa
      901 tgggcccctg ataatacaga atgggcagat tattataatg atacaaacta cactgatgaa
      961 gctttcaata ataaaagaga tctcgtaaca cagtttttta attcagttcc gaacgccagc
     1021 atggtatggg atttgggtgc aaatacagga gagttttcaa ggatagcttc acaaaagggg
     1081 gttaaaacca tagcgtttga tatagaccct tctgctgtag aaaaaaatta tttaaccata
     1141 attgaaaacg acgagaaaaa tcttctgcct ttggagctta atctgtgtaa tccaagccct
     1201 ggcataggat
//
LOCUS       DBNGSV010000013        19936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_971771, whole genome shotgun sequence.
ACCESSION   DBNGSV010000013 DBNGSV010000000
VERSION     DBNGSV010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19936
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_971771"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC159U_00610"
     CDS             <1..210
                     /locus_tag="AC159U_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3335590.1"
                     /translation="PDLLGVTKRREEEWLKKWLKSPDTMIFTDPIAKQMLKEYFVPMP
                     NLGLNDEQVEQLIIYFKKIDEQVKD"
     gene            234..2240
                     /gene="nosZ"
                     /locus_tag="AC159U_00615"
     CDS             234..2240
                     /gene="nosZ"
                     /locus_tag="AC159U_00615"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HIR3335591.1"
                     /translation="MKHNKRLIVSLCVIVAFAMGFLIFSSVSSDSKQGDLSPSAGEIA
                     QQRGLSPDDVTAALKTYVPTGKYDDYLMFSSGGHSGQVLVIGVPSMRLLKVIAAFTPE
                     PWQGFGTGSKESEELLKSGSMPIGKNRKLTWADTHHPALSETKGDYDGQFLFINDKAN
                     ARIAVIDLRDFEVKQMIKSPNIISDHGSTFVTPNTEYVIQGAQYATPIPYTDYAPLEE
                     YQEKYRGMIGFYKFNRQKGRIDMAKSFQIEVPPYWQDLCDAGKKVSEGWVFCGSLNSE
                     MATGGFGDGKPPFEAGVSQRDMDYLHVVNWKKAEAVVTAGKAKTISGIKVIPLQTAID
                     EGLLYFVPEPKSPHGADVSPDGNYIVISGKLDTHATIFGFDKIQKAIATKNFEGKDPF
                     GVPILDFNAVKAAQLEIGLGPLHTQFDDQGNAYTSVFIESTMAKWTLGEPYHSGEKAW
                     KLVEKIPVHYNIGHLVCAEGDTVSPDGKYCVSLNKWAIDRFTPVGPLHPQNFQLIDIS
                     GKKMQLLYDMPIGVGEPHYVQMIKADKLNPWLVYPQVGFNPSTMSVDPKAAKVGNEGV
                     VRNGNNVVVNMTAIRSHFNPEHIKVKEGDNVTIHLTSIERARDATHGFAIDGYNINLS
                     LEPGESSTVEFEADKPGVYPFYCTEFCSALHLEMAGYLLVEPKE"
     gene            2331..2711
                     /locus_tag="AC159U_00620"
     CDS             2331..2711
                     /locus_tag="AC159U_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335592.1"
                     /translation="MKLQKFLTCCLFLIVFVIAIPVNAADDAFKDSALSWQKQATGTR
                     AAVISVYEELTKIGDKGNADAKELIDDAVIQLGEGDKQLKAGDDLLAKEQFEKASYDY
                     NMAWQYYVKAATAGLNAKRILTGQ"
     gene            2745..3398
                     /locus_tag="AC159U_00625"
     CDS             2745..3398
                     /locus_tag="AC159U_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR3335593.1"
                     /translation="MKENELILGPRIPNELWQKERERFLIPTILMVTAAVLLLISIFL
                     PYWKLTLFAPQYPGGLKAELYVNRAAGDIEELDILNHYIGMKSMKEAAPLERSLSVAL
                     IVGVVLLTVAAIYVHSPIALFLTIPAIMFPAFFLGDLYYWLRNFGTNLDPNAPLNSVE
                     PFVPPLLGDSTIAQFKTTAQWEIGLHMSILASILIIAGLYFHRKAYKPLIEAKLKNE"
     gene            3436..4839
                     /gene="nosD"
                     /locus_tag="AC159U_00630"
     CDS             3436..4839
                     /gene="nosD"
                     /locus_tag="AC159U_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HIR3335594.1"
                     /translation="MKLYIYALMLIVSGGISAKANTYTVSPGGNLNSISKAILQAQNG
                     DSIIVDGGVYQEKLLISKEIKLIGINRPEIIGDGKGTIISVSAPNVTIQGFHISSTGS
                     NLNREDSAVVLDNSAGSVIKDNILEDVLFGIYLKNSPDCLVLHNKITGKDLPLPDRGD
                     SIRLWYSSGTAIKGNKISKTRDLVMWWSGDTVIEDNIVEQGRYGLHYMYSNNNIFKNN
                     IFRSNLVGGFLMYSKNIRFENNTFIQNQGVASGYGVGFKDLDLVTAVGNKFIDNRVGI
                     YMDNTPTSFDVWNTIEGNKIAFNDIGASLMPSIERNTIIGNNFIDNNEQVEVRGGGSL
                     KGNKWFEQSRGNYWSDYKGYDENSDGIGDFPYVAESLFEDMMDRNSVLRVFIHSPVTQ
                     ALDIAAKAFPVFRPAPKLKDEYPLTSPTEAHLVSDIIIKFNLRVLITYIILIVCPLLT
                     LIILIRPYKEQIIDRNS"
     gene            4823..5707
                     /locus_tag="AC159U_00635"
     CDS             4823..5707
                     /locus_tag="AC159U_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3335595.1"
                     /translation="MIEIAELTKSFGNFKALDCVSLSIADGESVCLLGENGAGKSTLI
                     KCILGMLEYSGQIKLNSIIIEPGSTEHRFDIGYIPQEPVFYDMKVDELISFFGILRRV
                     GNDKINESLKIAGLLEHRNKHTSELSGGLKQRLSFAIALISDPSVLILDEPTSNLDAS
                     ARLELMKLVKKLKETGKTVLFSSHRMDEVQFLADRVYLLKKGRVIKQSAENELTRQLG
                     LKTKIIVNVNVTDNQRTIEILKNSGFNHISKNGAGILVSIDNDDKTEALKKILDSGIA
                     INDINIEAPSMDEIMRSV"
     gene            5710..6549
                     /locus_tag="AC159U_00640"
     CDS             5710..6549
                     /locus_tag="AC159U_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3335596.1"
                     /translation="MNYTNILLITKKEVKESFQNRWFIIFTLAFSILAFMLSYVGTRN
                     LGYSEISGFGRTTASLINLALMLIPLMGLLLGSITISGERERGTLHYLLSHPIGKKEV
                     FAGKYVGLFFSLVFTITIGFALGGIAIFFKTGSSDIGKYILDLLITLILASSVLSIGI
                     LISVCTKKISKSIGIAIFVWFFLIILSDLGIIGSVILFNLGIEPTFYITLLNPTEVYK
                     ILSVLVLSTRFEILGPVGIYIFNTMGRYGSLVILSGVLALWSVVPLIIAYLYFCRFKR
                     EEI"
     gene            6546..6983
                     /locus_tag="AC159U_00645"
     CDS             6546..6983
                     /locus_tag="AC159U_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HIR3335597.1"
                     /translation="MKYPILISLILFVLSCSKESELLPVEIKYGQDLCAACSMIISEK
                     QYSAQYILSDGSIKKFDDIGCMIEHLKQEKNERGNISAVFVRDYIHKNWINTDIAHFV
                     HSNKIITPMGHGIAAFGTKEDAKNIETEIKGEYLGKLDILLDK"
     gene            complement(7195..7266)
                     /locus_tag="AC159U_00650"
     tRNA            complement(7195..7266)
                     /locus_tag="AC159U_00650"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7232..7234),aa:Met,seq:cat)
     gene            complement(7599..7982)
                     /locus_tag="AC159U_00655"
     CDS             complement(7599..7982)
                     /locus_tag="AC159U_00655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3335598.1"
                     /translation="MSSDVITLIGQGCIFEGNLTSPTSTRLDGTIKGNIDSQGTLVVG
                     EGGIISGEVKAAEISVHGRVEGNIHSEKLIIKSGGIVVGDINSKSFIIEDGGIYNGKC
                     LMQSSSYNGSAESADSSYEADSPNS"
     gene            complement(8047..8877)
                     /locus_tag="AC159U_00660"
     CDS             complement(8047..8877)
                     /locus_tag="AC159U_00660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3335599.1"
                     /translation="MDKNRLTVLLVSSNGSGTKSLSIDYKHIKIAAGVVLGFILISFV
                     SFAFSFNFYNEAKSYAGEKKEAMNLLVNQANLLNVMSSDKVVLQARLVDIEDKLLDMQ
                     SLLDKKGINKDLSIGGEYIPDDRWSLSYMDFMEKDINDLFQIMKSTPFGKPIKAKVNS
                     GFGHRKDPFAKKYAFHSGIDFDANMRQPVVATADGKVKHAGWYMSYGKTVIIKHNNGY
                     ETLYGHLDSLKVKKGEKVTTGQLIGKAGSTGRSTGPHLHYEIIKNGKKVNPREYLTFK
                     "
     gene            9283..9738
                     /locus_tag="AC159U_00665"
     CDS             9283..9738
                     /locus_tag="AC159U_00665"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR3335600.1"
                     /translation="MKSDNYYMQLAIDEAKKAKIRGEVPVGSIIVGNDGKVSAKGHNL
                     CEELSDPTAHAELIAIRKLSKKLGSWRLDGTTLFSTLQPCLMCMGAIVNARIGRLVYG
                     TDDPEYEGTNYIYELEDIYPLVKKLSVTNNVLQEDCALLLKSFFSAIRK"
     gene            complement(9743..10054)
                     /locus_tag="AC159U_00670"
     CDS             complement(9743..10054)
                     /locus_tag="AC159U_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR3335601.1"
                     /translation="MSNSKISKSEEEYFAKEDAEKKKRLEARLHDEQAKKKRAELKEI
                     CYLKCPKCAGELHELMFRGVAIDRCDDCNGVWLDDGELEKLAGAEDGNIVRDFMGMFK
                     K"
     gene            complement(10098..10766)
                     /locus_tag="AC159U_00675"
     CDS             complement(10098..10766)
                     /locus_tag="AC159U_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013583.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HIR3335602.1"
                     /translation="MSVELEVEGKTVSEAIINACEQMGVTRNQVDIEVLNEGSKGVLG
                     IGGRPAKVRAKIIQENVSEKGLKAKKVLDDILSYFCEDYSVNLRETADRIKLDVKMSD
                     NRGLIIGKSGEMLKSLEFLIGKISSRTTETGKGKRIYIDIEGYKRRKEDSISKMVRDS
                     VKKVRKNRKPVTLSPMSAYERRITYITLKREKGIRYDTKVDGDKKSITIIPESSNRQR
                     AESS"
     gene            complement(10782..12491)
                     /gene="yidC"
                     /locus_tag="AC159U_00680"
     CDS             complement(10782..12491)
                     /gene="yidC"
                     /locus_tag="AC159U_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3335603.1"
                     /translation="MNSDNKINYIIFFVLSFAIILGYSILFPPPKPVVEETAQKEKIS
                     DSIDDELIDNTGTVSKSIETPKSKDENIFKRLDDKSSPYATSENLITVESPLYTGKID
                     PLGARIVEWKLDKYKESTQNGSQEYNLFSDSPPGFNLGVQASFEDIPNPIPFSYAGSK
                     NIVLKDDAKELEFVWKSPGGITISKTYNISPNSYLVTSTIRIINSTGKTIKHIVSVDT
                     FSSALKVDSQGRSKEFIALVSDEIQKESSNPEQTIEYAGKIEWFGFLDKYFLYTFLPE
                     SDDTNKVKFSSISGGDELVRATYSYPAYELKPGNERPYKSKLYLGPLQVDSLKQADPE
                     LKHAMDFGWFDFLAKPVLSLLNYMNKYFNNYGISIIVITILIRVIFLPLTIKSMASMK
                     EVQVKMQTLKPKIDALKEKYKDDKQKQNQEMMNMYTAHGVNPLSSLGGCLPMLIQIPV
                     FIALYEVLLNSIDLRHSAFLWINDLSEAEVLFDIPAIGIPFRILPLAMGVSWFISQKM
                     TPMTTPGSDQMELQMKMMQFMPIIFTVMFWGLPSGLVLYWTVSNILSIVQQLYVNKKT
                     AKH"
     gene            complement(12524..12745)
                     /gene="yidD"
                     /locus_tag="AC159U_00685"
     CDS             complement(12524..12745)
                     /gene="yidD"
                     /locus_tag="AC159U_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3335604.1"
                     /translation="MIRNALLKIMLGGLRVYKTVSFLLPCACRFYPSCSVYCTHALEK
                     HGIFRGLYLSVKRILRCNPFSFGGYDPVK"
     gene            complement(12748..13098)
                     /gene="rnpA"
                     /locus_tag="AC159U_00690"
     CDS             complement(12748..13098)
                     /gene="rnpA"
                     /locus_tag="AC159U_00690"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3335605.1"
                     /translation="MKAVSSETFPKHLRIHNTLDFKRLFAHGSRSNLESVRVITLDNN
                     LGYPRLGIVASKKNLPKSVMRSKFKRAVREVFRKNKEYLDSKDYLVINLRKASDKGRL
                     YKARDFEGYIKSIH"
     gene            complement(13095..13244)
                     /gene="rpmH"
                     /locus_tag="AC159U_00695"
     CDS             complement(13095..13244)
                     /gene="rpmH"
                     /locus_tag="AC159U_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3335606.1"
                     /translation="MKRTYQPHAKKRLRTHGFRERMSTKAGRRVLKRRLSKGRHSLAV
                     QAQKK"
     gene            complement(13326..14720)
                     /locus_tag="AC159U_00700"
     CDS             complement(13326..14720)
                     /locus_tag="AC159U_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335607.1"
                     /translation="MAKKSDIETFMSKGYQEAINYPLFDSIFNRRSRRFGLGMELPNS
                     TLQYKSDAPPIPLDEFEEALIAWSGTGLTGLCLADLPPENGIDLLCQWTGRTWPSACN
                     NHGTELFFTNDEGLYHIDVKHMLPKNHELDMFFKMSREQRVERVMELYRESLVKLEDG
                     RADLPDKMPGLFDFNQWNTNKPGTSVYIPVTDITEEYINLLLLYCSNTYGFTIIDDLT
                     GKPAGVDKWVKKGRLKEQVKLSLFDLEVRILTGLNVEQAFICQNMSLALQALGLAGWT
                     FSGFIPRFAMGAAPDLFKGLGFRFIQPKKGAIDPPTTVPVGRDGVFEAYSPPYYKDMN
                     EAFDVFFEHKNEAWKEDKPFPYTEPDKHLMQAPKPTETTKEIVKDVLNYIYDTYGRFP
                     AFVDPMYMRLVFQAMHIDVDFYEKYYPKGSYTDLHLNHFKHFHSDIKDPFAHKTKKDK
                     KVAASGAKKKKARA"
     gene            14861..17452
                     /gene="mutS"
                     /locus_tag="AC159U_00705"
     CDS             14861..17452
                     /gene="mutS"
                     /locus_tag="AC159U_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3335608.1"
                     /translation="MSTETPMVRQYKKIKLEHPDAILFFRLGDFYEMFYEDAKTASKV
                     LGITLTSRNKSDTNPIPLCGVPFHSAEPYLAKLLEHGHKVAICEQIEDPKLARGIVER
                     KVVKILTPGVIVDTEHLEDKTNNYLTCIAKYGEHRAIATADISTGELRAGTFSSAETL
                     KSELTNLQPKEILIPCDTDGYDFINDLSNDIWKPVITKKEEYLWELQSCDELLKNYLG
                     VKTLQAFNLENKPDCIVALGVLINYLLSTQIDDMPPLKNPVFYDRNNFLQIDGTTMSN
                     LEILRTIRGDTKSGTLLSVIDMTLCAMGARLLRQWINYPLTDSVKINKRLQRVDVLSK
                     DPALKRNLRASLKEISDIERIITRISTNSAKPRDLGAIRDSIVFIKEIKNVLIDSNIL
                     ELGELEQKLDSLQDIYEIISESIVSEPPNTLRTPPVIKEGFNKELDSLRSLTKDGKKW
                     IAELEAKEKEATGINFLKIGYNKVFGYYIEVTKANIHLVPESYIRKQTLVNAERFITP
                     DLKEQEEKIVGAEEKILDLERTLFEQIRKQVEAHSHRIRKTSAVIAEIDVLCGLSETA
                     ERNSYVKPEINNSDIIDLHGSRHPVIESIITDERFVPNDIKFDCKENQLLLITGPNMA
                     EKSTLIRQAALCVLMAQIGSFVPAKSASIGVVDKIFTRVGASDNLSKGQSTFMVEMVE
                     TAYILRNATDKSLVILDEIGRGTSTFDGMSIAWSVAEFLHDLGCKTLFATHYHELAEL
                     SKDKERVKNYNVCVKESDDKIVFLRKLIEGAASHSYGIEVAKLAGIPGEVISSAKKVL
                     YNLEKTQSDIKSSISEDQLKLFNNKQEDKKETELIDQLKSLDLDNMTPMQALSKIAEL
                     KEKLKET"
     gene            17463..17873
                     /locus_tag="AC159U_00710"
     CDS             17463..17873
                     /locus_tag="AC159U_00710"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3335609.1"
                     /translation="MKLLEHISNIFNKKNELDNKSRGELGEKKACKFIKKNGYEVLEK
                     NYRTKFGEIDIVASEHDTICFIEVKARSNSDYGNPEEFVTKKKQEKLWKTASIYIDNN
                     PSDLENYRFDVIAIDFESNNVEIFKNAFEPEVSF"
     gene            complement(17874..18209)
                     /locus_tag="AC159U_00715"
     CDS             complement(17874..18209)
                     /locus_tag="AC159U_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335610.1"
                     /translation="MDKLYKLLEEIRDQFEQEGWYCVLTDKSSFNQNPDLKTDFDVGF
                     DYRDLRALSIIDFENRIVLNSYFYIVDDKTFYLHGDVTHSDKPVAYQIENIDQEKLIE
                     DIKRVINYY"
     gene            18327..19271
                     /gene="ispH"
                     /locus_tag="AC159U_00720"
                     /gene_synonym="lytB"
     CDS             18327..19271
                     /gene="ispH"
                     /locus_tag="AC159U_00720"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3335611.1"
                     /translation="MKVIVAKPRGFCAGVDRAIEIVERALEMYGPPIYVRHAIVHNKR
                     VVEKLKAKGVVFVEELEEIKEKDSRVIFSAHGVSPAVWNEAKDRSLQSVDAVCPLVTK
                     VHSEVKHYADKGYSIVLIGHRNHVEVIGTSGEAPEKVIVVESVEEAEKLELPDPNKVA
                     YVTQTTLSVDDTRDIVETLKARFPKIVAPSKLDICYATQNRQDAVKQLAKHSDMIFIM
                     GSPESSNSNRLVEVAKSCGVEKASLIEGIEDLDKDMFSDDMTIGLSSGASTPETVVQE
                     VISKLREFGAESVEELDGKEEFTRFPFEEHLEFRSSYN"
     gene            complement(19273..19608)
                     /locus_tag="AC159U_00725"
     CDS             complement(19273..19608)
                     /locus_tag="AC159U_00725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3335612.1"
                     /translation="MAAYIPKKGDFVTLTFDPQSGREQKGRRPALIISNDLFNKATGL
                     AIACPITNTDRNVPFHVKVPSESQLTGFIMVDQVKSIDYISRKIKFVEKASPEIFNEV
                     LEILDALIY"
     gene            complement(19609..>19936)
                     /locus_tag="AC159U_00730"
     CDS             complement(19609..>19936)
                     /locus_tag="AC159U_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3335613.1"
                     /translation="SNILIRYVEIIKLQCILCIYFFKEIVMVTKIQKWGNSQGLRVSK
                     YILEKAKIEVGDEVDMTVKDGKIIIKSVKPKRGKKKLEQLVSKIDKNYNPSEEDWGAP
                     VGKEVW"
BASE COUNT         6279 a         3841 c         3778 g         6038 t
ORIGIN      
        1 cccgatcttt taggtgttac aaaacgaaga gaagaagagt ggttaaagaa gtggctcaaa
       61 tcccctgaca caatgatttt tacagatccc attgctaagc aaatgcttaa ggaatatttc
      121 gttccaatgc ccaatcttgg attaaatgat gaacaagtag aacagcttat tatctatttt
      181 aagaaaattg acgaacaggt taaagattaa tctttattaa aggaggaaag aatatgaaac
      241 acaataaaag acttatagta agcctatgtg taattgttgc atttgccatg ggttttctaa
      301 tattctcttc tgtctcttct gacagtaaac agggtgatct ttcaccatct gccggtgaaa
      361 tcgcacagca gaggggttta tcacctgatg atgttacagc tgctttaaag acttatgttc
      421 caaccggtaa gtatgatgac tatttgatgt tctcatcagg aggtcactca ggacaggttc
      481 ttgtcattgg tgtaccttca atgcgtcttt taaaggttat agccgcattc acgccggaac
      541 catggcaagg atttggaaca ggttcaaaag aatctgaaga attattaaaa tccggctcca
      601 tgcctattgg aaaaaacaga aaactaacct gggctgacac gcaccatccg gcgctgtctg
      661 aaacaaaagg cgattatgac ggacagttcc tgtttattaa cgacaaggct aacgcgagga
      721 ttgcagtaat agatctgcgt gacttcgagg ttaagcaaat gattaaaagc cccaatataa
      781 tctcagacca cggatcaacc tttgtcactc caaataccga atacgttatt cagggtgcac
      841 agtatgcaac accaatccct tataccgatt atgctccttt agaagagtat caggaaaaat
      901 acagaggaat gattggtttt tataaattta accgtcagaa gggaagaatt gatatggcca
      961 aatcatttca gatagaagtg ccgccatact ggcaggacct gtgtgatgct ggcaagaagg
     1021 tcagtgaagg ttgggtgttc tgcggctctt tgaacagtga aatggcaact ggtggttttg
     1081 gtgacggaaa acctcctttt gaagcaggcg tatcccaaag agatatggac tacctgcatg
     1141 ttgtaaactg gaaaaaagcc gaagctgttg taacagcagg taaagcaaag acaatttctg
     1201 gtattaaggt catacctttg cagacagcta tcgacgaagg ccttctgtac tttgtgcccg
     1261 aacctaagag tccgcacggt gcagacgtat ctcctgacgg aaactatata gtaatctccg
     1321 gtaagctcga tactcatgca acaatatttg gatttgataa gattcaaaaa gcgattgcaa
     1381 ccaagaattt tgaagggaag gatccttttg gagttccaat tcttgatttt aatgccgtta
     1441 aagcagcaca gcttgaaatc ggtcttggac cactgcatac ccagttcgat gaccagggca
     1501 acgcatacac cagtgttttt attgaaagca ccatggctaa atggaccctt ggagagcctt
     1561 atcattcggg cgaaaaagcc tggaaacttg ttgaaaaaat acctgtacac tacaacatcg
     1621 gccacctcgt ttgcgcagaa ggtgacacag tatctcccga tggtaaatac tgtgtttcac
     1681 ttaataaatg ggcaatagac aggtttactc ctgtaggtcc gctgcaccca caaaactttc
     1741 agttaattga tatttcaggg aagaaaatgc agctgcttta tgacatgcca ataggagtcg
     1801 gtgaacctca ttatgttcaa atgattaagg ctgacaagct aaacccatgg cttgtttatc
     1861 cacaagttgg atttaaccca tcgactatgt cagtcgatcc aaaagccgca aaagtaggca
     1921 atgaaggggt agtcagaaat ggaaacaatg tcgtagtaaa catgacggct ataaggagtc
     1981 actttaatcc cgagcatatc aaggttaaag aaggagataa tgtaactata catctaacaa
     2041 gcatagaacg tgccagggat gccacgcatg ggtttgcaat tgatggctat aatatcaacc
     2101 tcagtcttga accgggtgaa tcaagcacgg ttgaatttga agccgataag cctggggttt
     2161 atccatttta ctgtaccgag ttctgttcgg cactgcactt agaaatggca ggctatctgc
     2221 ttgttgaacc aaaagaataa acttctcaca tgtaccgttt tgatcatctc ttctcttacc
     2281 cccagtatgg tcaaggcggt acatgaaaat agataaggag ggatcataaa atgaaattac
     2341 aaaagttctt aacatgttgt ttgtttttaa ttgtttttgt tatagcgatt cctgtaaatg
     2401 ccgcagacga cgcattcaaa gacagcgccc tttcatggca gaagcaggct accggcacca
     2461 gagcagctgt aatatcagta tatgaagagc tcaccaaaat tggtgataag ggtaatgcag
     2521 atgcaaagga gttaattgat gacgcggtaa ttcagctggg agaaggtgac aaacaactca
     2581 aagccggaga tgacctgctt gcaaaggagc agtttgaaaa agcgtcatat gactataaca
     2641 tggcctggca gtattatgtt aaggctgcaa ccgccgggct taatgcaaaa cgcattctca
     2701 caggacagta actatacacc attaaaggag atggaggata tataatgaaa gagaatgaac
     2761 taatattagg gccgcgtata ccaaatgaac tctggcagaa agagagggaa agatttctta
     2821 tcccaaccat attaatggta acagcagcag ttttgcttct aatctctata tttcttccct
     2881 actggaagct cacactattc gcacctcagt atcccggtgg gttaaaagca gagctttatg
     2941 taaaccgtgc tgcaggggat attgaggaac ttgatatact caatcactac atcggcatga
     3001 aatcaatgaa ggaagctgct ccccttgaaa ggtccttaag cgtagctctt atagtaggag
     3061 tagtcttgct taccgtagct gcaatatatg ttcacagccc catagctttg ttcctcacaa
     3121 tccctgcgat tatgttcccg gcgtttttcc tgggggatct gtattattgg ctgagaaatt
     3181 ttggaacgaa tcttgatcca aatgctccgc taaattcagt tgaacctttt gtgcctccac
     3241 tgcttggaga cagtacaata gcacagttta aaacaaccgc tcagtgggaa atcgggttgc
     3301 acatgtccat tcttgcatcg atcttaatta ttgcaggtct ttactttcat cgtaaagctt
     3361 ataaacctct tatagaagca aagcttaaga atgaatagca gttagcagta ggatttaatc
     3421 tgaatcggtg atattatgaa actatatatt tatgccttaa tgcttattgt ttcaggcggc
     3481 atatccgcaa aggccaacac atataccgta tcaccgggtg gtaatcttaa ctcaatttct
     3541 aaagctatac ttcaggcaca aaatggtgat tcaatcattg tagatggagg agtgtatcaa
     3601 gagaaattat taataagcaa agaaataaaa ctaatcggca taaacagacc tgagataatc
     3661 ggggatggaa agggtacaat tataagtgtt agtgctccaa atgtaacaat acagggtttt
     3721 catatctcaa gtacaggcag taatctaaac cgcgaagact ctgctgttgt tcttgataac
     3781 tcagcaggct ctgtaatcaa ggacaatata ctcgaagatg tactctttgg aatctacctt
     3841 aaaaactcac cagactgttt ggtacttcat aacaaaataa caggcaagga tcttccattg
     3901 cccgataggg gagacagcat aaggctgtgg tacagctccg gtactgctat taaaggaaac
     3961 aaaataagta aaacacgaga ccttgttatg tggtggtcgg gagatacggt gatcgaagat
     4021 aatatcgtag aacaaggaag atatggtctg cactatatgt acagcaacaa caatatattt
     4081 aagaacaata tcttcaggtc aaacctcgta ggcggctttc ttatgtacag caagaacatt
     4141 cggtttgaaa ataacacttt catccaaaac cagggtgttg caagcggata cggggtaggg
     4201 tttaaagacc tcgaccttgt aacagccgtg ggcaataaat ttattgataa ccgcgtggga
     4261 atatatatgg acaatactcc cacttctttt gatgtgtgga acactattga gggaaacaaa
     4321 atagctttta atgatattgg ggcttctctt atgccctcta tagaaagaaa cacgattatc
     4381 ggcaataatt ttatagataa taacgaacag gtagaggtca gaggcggggg ctcgcttaag
     4441 ggtaacaagt ggtttgaaca aagcagagga aactactgga gtgactataa agggtatgat
     4501 gaaaacagtg acggaatagg ggattttcct tatgttgcag agagcctgtt tgaagatatg
     4561 atggaccgta attcagtctt aagggtgttt attcacagcc ccgttacaca agcactcgac
     4621 attgctgcaa aagcattccc ggtatttaga cctgccccga aattgaaaga tgaatatccc
     4681 cttacttccc ctactgaggc tcatttagtg tcagatataa taataaaatt taatttaaga
     4741 gtattaatta catatataat tcttatagtc tgtcccctgc ttactcttat aatattgatt
     4801 agaccgtaca aggagcagat tattgataga aatagctgaa cttacaaaat catttgggaa
     4861 ttttaaggct cttgactgcg tatcactttc cattgcagat ggagaatctg tatgtctgct
     4921 cggtgaaaat ggtgccggta aaagtaccct catcaaatgc atactcggca tgctagagta
     4981 ctccgggcag attaaactaa acagcataat aatagagccg ggatctactg aacacaggtt
     5041 tgatatagga tacattccgc aagaacctgt tttttatgac atgaaggtag acgaattaat
     5101 atcgtttttt ggaatactta gaagagttgg taatgacaaa atcaatgagt cattaaaaat
     5161 tgcaggtctt ttagaacaca gaaataaaca tacatctgag ctttcgggag gattaaaaca
     5221 gcggctttcc tttgcaattg cccttatttc cgacccctca gtactcatac tcgatgaacc
     5281 cacttcaaac ctggatgcat cagcaagact cgaacttatg aagcttgtaa aaaaacttaa
     5341 agaaaccgga aaaaccgtac tgttttcatc gcaccgtatg gatgaagtgc agtttctcgc
     5401 agacagggtg tatctactaa aaaaaggcag agttataaag caaagcgctg aaaatgaact
     5461 tacaagacag cttggactta aaacaaaaat cattgtcaat gtgaatgtga cagataacca
     5521 aaggactata gaaatcctta aaaactcagg gttcaatcac attagtaaaa acggggcggg
     5581 aattttagtg agcattgaca atgatgataa gactgaggca ctaaaaaaga tattggatag
     5641 cggtatagca ataaatgata tcaacataga agctccatcg atggacgaga taatgaggtc
     5701 tgtataaaga tgaactatac aaatatatta cttattacaa aaaaggaagt taaggaatcc
     5761 tttcagaacc gctggtttat tatttttaca ctcgcatttt ccatactcgc ttttatgctc
     5821 tcatatgtag gaacaagaaa tctgggctac tctgaaatat cgggttttgg caggactacg
     5881 gcaagcctca taaaccttgc gcttatgctt atccccctca tgggtcttct actcggttct
     5941 attaccatat caggagaaag ggaaaggggc acactgcatt atttgctttc acatcccatt
     6001 ggaaaaaaag aggtttttgc aggcaagtat gtggggctgt ttttttcact tgtgtttacc
     6061 attacaattg gatttgcact gggtggaata gcgatattct ttaagacagg ctcttcggat
     6121 ataggcaagt atattttaga cctgctaatt acccttatcc tcgcatcatc cgtgcttagt
     6181 atcgggatac ttatttctgt atgtacaaag aaaatatcca agtccatagg aatagccatt
     6241 tttgtctggt tctttctcat catactgagt gatctgggta taataggatc ggtaatttta
     6301 tttaaccttg gaatagaacc gactttttat ataactcttt taaaccctac ggaggtctat
     6361 aaaatacttt cagtactggt actcagtaca cgctttgaaa tactgggacc agtgggtatt
     6421 tatattttta atacaatggg cagatacgga tcgcttgtaa tattatctgg tgttcttgca
     6481 ctatggtctg ttgttccact tataattgcg tatctatatt tttgcagatt taaaagagaa
     6541 gaaatatgaa atatcctatt ttgatttctt tgatcctatt tgtattatca tgttctaagg
     6601 aaagtgaact actccctgta gagataaagt acggacaaga cttatgtgct gcctgctcga
     6661 tgataataag cgaaaagcag tactcggcgc aatatatatt atccgacggc agtataaaaa
     6721 agtttgatga tataggatgt atgatagaac atttaaaaca agaaaaaaat gaacgtggta
     6781 atatatctgc agtatttgtt cgggattaca tacacaagaa ctggataaac acagatatag
     6841 cccactttgt acatagtaat aaaattatta caccaatggg acatggtata gccgcctttg
     6901 gaactaaaga agatgcaaaa aatattgaga ccgaaatcaa aggtgaatat ttaggtaaac
     6961 ttgatatatt attagataaa taactttctc aaaatgctat atatacaatg actacagcca
     7021 gtgttttgaa aattgttaca gacatatgtc actctaaata cggtttttca tttttataac
     7081 tatttttaat tttaaaagtg caaaacgcat tttattaaca gtaaagacta ctaactttca
     7141 ttaacactat attttgccga gggcacgcat tgggaatata attgaaataa ataatggcgg
     7201 cgggaggact cgaacctccg acaggcgcct tatgagagcg ctgctctacc tctgagctac
     7261 gccgccagag aaggtagtaa atcaattttg acccgcaatt atatcacttt gtatgccaga
     7321 ttcaataagt gtaaaatttt taggatcaat tacttgacat attaagaaat ttactttatt
     7381 ttaatattgt aagttaattg taagacttaa tatataatat atttaattag tagtacggta
     7441 acatcctcca tcctccttaa agtgggaatg ttttcaagag ctgaaaacat tcccactttt
     7501 tattttgtct caattaaaac tctgagaact tatgaaggaa gatagtaaat caaattaaag
     7561 aaatctaata tattaagtcg gtttttttaa gtcacaaatc agctgtttgg actgtctgct
     7621 tcataactgc tgtcagcaga ttctgcgctt ccgttataac tggaagactg cataaggcat
     7681 ttaccgttat aaataccgcc atcttcgatg ataaaagatt tggaattgat atcacctaca
     7741 acaatcccac cgctttttat tatcagcttt tctgaatgaa tattcccttc taccctgcca
     7801 tgcacagaaa tctctgctgc ttttacctct ccggatataa tcccgccttc tcccacaaca
     7861 agtgttccct gactgtcaat atttcctttt atagtaccgt caagtcttgt agaagtgggt
     7921 gaagtaaggt ttccctcaaa aatacacccc tggccgatta aagttattac gtcgctgctc
     7981 atatagtcac tcactgtttg agtaatatca ctactactct tttttttatt taatattccc
     8041 ataagattat ttaaatgtta aatactctct cggatttact tttttcccgt ttttaatgat
     8101 ttcataatgc aggtgtggtc ctgtggatct gccagtagaa ccggctttac ctataagctg
     8161 acctgttgtg accttttcac cttttttaac ctttaatgag tccaaatgac cgtatagtgt
     8221 ttcatatccg ttgttgtgct tgatgattac agttttacca tagctcatat accacccggc
     8281 gtgcttaact tttccatcag ctgtagctac taccggctgg cgcatattgg catcaaaatc
     8341 tatacctgaa tgaaatgcgt actttttagc aaacgggtct tttctgtgtc caaaacctga
     8401 attcaccttg gcttttatgg gtttgccaaa tggggtgctt ttcataatct ggaaaagatc
     8461 attgatatct ttttccataa aatccatgta tgacaggctc catctgtcat caggaatata
     8521 ctctccacct atagaaagat ctttattaat tcctttttta tcgagaaggc tctgcatatc
     8581 aagcagttta tcttctatat caaccagcct tgcctgaagc actactttat ccgagctcat
     8641 tacatttaga agatttgcct gatttacaag taggttcatg gcctctttct tctcacctgc
     8701 ataagattta gcttcattat aaaaattaaa actaaaagca aaagaaacaa aagatattag
     8761 aataaaacct agaacaacac cagctgcaat ttttatatgt ttatagtcaa tagataatga
     8821 ttttgtcccc gaaccatttg agcttacgag caaaactgtc aaacgatttt tatccattac
     8881 tctccatctc cttataacac acccgattaa atcattcttt aatgactaat atacgccttt
     8941 ataccttaac cccggtcttg aaaagaataa tgctgtgtta tataactcta attattatga
     9001 ttttttaata atattgcaag agcccacctg ctctatctac acttttataa tacaaaaaca
     9061 cttagaagtc aatacaaaat attttattag ctctatttta tttttatact tgacaaataa
     9121 agtaatttac tttattttta ttaacagagt taatgaaaat acttaaatca tcctccatcc
     9181 tcctttaaga taacgggaat ggttattgac ccaaataatc attcccgtta atttttaact
     9241 tagtacatca tataaatcct atcatcaaga tttccgcata atatgaaaag tgataattat
     9301 tatatgcagc ttgccataga tgaagcaaaa aaggctaaga taagaggtga ggtgcctgtt
     9361 ggatcaataa ttgttggcaa tgacggtaaa gtatccgcaa agggccataa tttatgcgaa
     9421 gaactatcag atccgaccgc acatgcagag ctaattgcta taagaaaact ctcaaaaaaa
     9481 ttagggtcct ggaggcttga cggtactact ctgttctcga ctctgcagcc atgccttatg
     9541 tgtatgggtg caatagtaaa tgcgaggata ggcagactgg tttacggtac ggatgacccg
     9601 gaatatgaag gaacaaatta tatatacgaa ttagaagata tttatcccct tgttaaaaaa
     9661 ttatccgtta cgaataatgt attacaagaa gactgcgctt tgttgttaaa aagttttttc
     9721 agtgcaataa gaaaatagaa tattatttct taaacattcc catgaaatct cttactatat
     9781 tgccgtcttc agcccctgca agcttctcaa gctcaccgtc atcaagccat actccgttac
     9841 aatcatcaca cctatcaatt gcaacgcctc taaacattaa ttcatgcagt tcacctgcgc
     9901 atttggggca tttgagataa catatttcct taagctcagc acgctttttt ttagcctgct
     9961 cgtcatgaag cctggcttct aacctctttt tcttctctgc atcttctttt gcaaaatatt
    10021 cttcttctga tttactgatt ttactgttac tcataatttt ttacctttta aatagaatat
    10081 aaaattatta tattaaccta tgatgattct gctctctgcc tgttactgct ctcaggtatt
    10141 attgtaatgc tttttttatc tccatcaacc tttgtatcat acctgattcc cttttcccgc
    10201 ttaagcgtaa tataggttat ccttctctca taagccgaca tggggcttaa tgttacaggt
    10261 tttctgtttt ttcttacttt cttgaccgaa tccctaacca tctttgaaat ggaatcctct
    10321 tttctccttt tatatccttc aatatcgata taaatccttt tgccttttcc tgtttcagtg
    10381 gttctgctcg atattttgcc tataagaaac tcaagggatt ttaacatctc accgctcttg
    10441 ccgattataa gccctctgtt atcagacatt tttacatcaa gcttgatcct gtctgctgtt
    10501 tcccttaagt tcacagagta gtcttcgcaa aagtatgaaa gtatatcatc gagcactttt
    10561 ttggccttca gacccttttc actcacattt tcctgaataa tctttgccct tacctttgca
    10621 ggtcttcccc caattccaag caccccttta gatccctcat ttaatacttc aatatcaacc
    10681 tggttccttg taacacccat ctgttcacag gcattaataa ttgcctctga aactgtttta
    10741 ccttcaacct caagttcaac tgacatcgta taaatcctcc attaatgttt agcagtcttt
    10801 ttgttaacat agagctgctg aacaatcgaa agtatgttgc ttacagtcca atacagaaca
    10861 agccccgaag gcagacccca aaacatgact gtaaatatga taggcataaa ctgcatcatc
    10921 ttcatctgaa gctccatttg atccgacccg ggtgtcgtca taggagtcat tttctgagat
    10981 ataaaccacg atacacccat tgcaagagga aggattctaa aaggtattcc tattgctggt
    11041 atatcaaaca gcacttcagc ttcggacaga tcgtttatcc ataaaaacgc actatgtctt
    11101 aaatcgatag aatttagcag tacttcgtaa agtgcaataa acaccggtat ctgaataagc
    11161 ataggcagac atccgcctag actgcttagg gggtttaccc catgggcagt atacatattc
    11221 atcatttcct gattttgctt ttgcttatcg tctttgtatt tttcttttag cgcatctatt
    11281 tttggcttca gtgtctgcat ctttacctga acctctttca ttgaagccat gctctttata
    11341 gttagcggaa gaaaaatgac acgaatcaat atagtaataa ctattattga aataccgtag
    11401 ttattgaaat acttattcat gtaatttagg agtgaaagca cgggctttgc caggaagtca
    11461 aaccacccaa aatccattgc gtgtttaagc tcaggatctg cctgttttag tgaatccacc
    11521 tgaagcggcc caaggtagag cttggatttg tatggtctct cattaccggg tttaagttca
    11581 taagctggat aagaatacgt tgcccgcact aattcgtctc cgccggaaat tgaggaaaac
    11641 ttaaccttat tcgtatcgtc tgattcagga aggaaggtgt ataaaaaata tttatccaaa
    11701 aaaccaaacc actctatttt tccggcatac tctattgttt gctcggggtt actggattct
    11761 ttttgtattt catcagaaac cagtgcaatg aattccttgc ttcttccctg cgaatctacc
    11821 ttaagcgcac tgctgaatgt atctacactc acaatgtgtt taatcgtttt accggtgctg
    11881 tttattatcc tgatggtaga ggtaacaagg tagctgtttg ggcttatatt atatgttttt
    11941 gaaatagtta ttccacctgg tgatttccac acaaattcca gttcctttgc gtcatcttta
    12001 agaactatat ttttactgcc cgcatagcta aacggtatgg ggtttggaat atcttcaaaa
    12061 cttgcttgta ctccaagatt aaagcccgga ggagaatcgc taaacaggtt gtattcttgt
    12121 gagccatttt gagtgctttc tttatattta tcgagtttcc attctactat ccttgcaccc
    12181 agaggatcaa ttttaccagt ataaagaggt gactcaactg ttatgaggtt ctcagaagta
    12241 gcataggggc ttgatttatc atcaagcctt ttaaaaatat tctcgtcttt agatttcggt
    12301 gtttctatac tcttacttac ggtgcccgta ttatcaatga gttcatcatc aatactgtca
    12361 gatatttttt ctttttgtgc agtctcttca acaacaggtt ttggtggcgg aaaaagtatt
    12421 gagtagccta gaatgattgc aaaagaaagt acaaaaaata taatataatt aattttatta
    12481 tcgctgttca ttacttatct ctaattgtta ttatttctct tatttattta accggatcgt
    12541 atccgccaaa gctaaaaggg ttgcatctca atattcgttt aactgaaaga tatagccccc
    12601 tgaaaattcc gtgtttttca agcgcatgcg tacaataaac cgagcagctt ggataaaatc
    12661 tgcatgcaca gggaaggagg aaagaaaccg tcttataaac tctaagaccc ccaagcataa
    12721 tttttagcaa cgcatttctt atcataatca atgaatactc tttatatatc cctcaaagtc
    12781 ccgggcttta tataaccgcc ctttatcact tgcttttctc aaatttatta ccaaataatc
    12841 tttactatcc agatactcct tattctttct gaatacctct ctaactgctc ttttaaattt
    12901 gcttctcatg acagattttg gaaggttttt cttgcttgct acgattccca atcgtggata
    12961 tcccaggttg ttatccaagg taataactct tacggactca agattgcttc tactgccatg
    13021 ggcaaacaat ctcttgaaat ccagtgtgtt atgtatcctt agatgtttag gaaaagtttc
    13081 agaagaaaca gccttcattt cttctgtgct tggactgcca gactgtgcct gcctttggat
    13141 agccttcttt ttagtactct tctaccagct ttggtactca ttctttcacg aaatccatgt
    13201 gtacgtaaac gcttcttcgc atgcggttga tatgttcttt tcattgtata taacccctaa
    13261 aatgtaaaaa tcttgtataa aaacccctag tctaccatgt aatttacaaa aatctattat
    13321 gctgattaag ctctggcctt tttcttcttg gcgcccgaag ctgcaacctt cttgtctttt
    13381 ttagttttgt gcgcaaaagg atccttaatg tcggaatgaa agtgcttgaa atggtttaga
    13441 tgaagatcgg tataagagcc ctttggataa tacttttcat aaaaatcaac gtctatatgc
    13501 attgcctgaa atacaagcct catgtacatt ggatcaacaa aggcagggaa cctgccatat
    13561 gtgtcatata tatagttcag aacgtccttt acaatttctt ttgtagtttc agtaggtttt
    13621 ggagcctgca tcaggtgctt gtcgggttca gtgtatggga acggcttgtc ttctttccat
    13681 gcctcgtttt tatgctcaaa aaatacatcg aatgcctcgt tcatatcctt ataataaggc
    13741 ggtgaatatg cttcaaacac gccgtctctt cctacaggaa cagtagtcgg aggatcgatt
    13801 gcaccctttt tgggctgtat aaacctaaaa ccaagaccct taaacagatc aggagcagca
    13861 cccatggcaa accttgggat aaacccgctg aatgtccagc cggcaagtcc aagagcctga
    13921 agcgcaaggc tcatgttctg acagataaat gcctgttcta cgtttaagcc cgtaaggatt
    13981 cttacctcca gatcaaaaag tgacagctta acctgctctt ttagtcttcc cttcttgacc
    14041 cacttgtcta caccggcggg ttttccggta aggtcatcaa taatagtaaa tccgtatgtg
    14101 ttactgcaat acagaagcag caaattaata tattcttcag taatatccgt tacaggtata
    14161 tagacagatg ttccgggctt gttggtattc cactggttaa aatcaaaaag gcccggcatt
    14221 ttatccggta gatcggccct tccgtcttca agcttaacaa ggctttctct gtaaagctcc
    14281 atcacccttt ccactctttg ctctctactc atcttaaaaa acatatcaag ctcgtggttt
    14341 ttagggagca tgtgttttac atctatatga tataaaccct cgtcgtttgt aaaaaaaagc
    14401 tccgttccgt ggttattaca tgccgatggc catgttcttc ctgtccactg gcaaagaaga
    14461 tcaatcccgt tttcaggtgg aagatcggca aggcaaagcc cggtaagccc ggtacctgac
    14521 catgcgatta gagcctcttc gaattcatct agcggtattg gaggagcatc ggacttatac
    14581 tgcagagtac tattaggaag ttccatccca agtccaaacc ttcttgatct cctgttaaat
    14641 atagaatcaa aaagcggata gtttatagcc tcctgatatc ctttactcat aaatgtttca
    14701 atatctgatt tcttagccat tttgttcctc caatattgtt aaatcattat aaaaaaccag
    14761 ttaagactct aaaatattaa taacataaaa tcaaccaaaa tttaaacata atttaatatt
    14821 agattgtagt tagtaaaccc agtcgatata atcttaagtc atgtctactg aaacacctat
    14881 ggtaaggcag tacaaaaaga taaagcttga gcaccccgat gccattcttt ttttccgtct
    14941 gggcgacttt tatgagatgt tttatgaaga tgcaaagact gcatcaaaag tgcttggaat
    15001 tacccttaca tcgagaaata agtcagatac aaacccgatt cctctttgcg gagtgccgtt
    15061 tcatagcgcc gagccttatt tggcaaaact tttagagcac ggacacaagg tagcaatctg
    15121 tgaacaaata gaagatccga agcttgccag gggaattgtg gagagaaagg tagtaaagat
    15181 attaacacca ggagtaatag ttgatactga acacctggaa gataaaacca ataactatct
    15241 aacctgtatt gcaaaatatg gggagcaccg ggcaattgcc acagctgata tatctacagg
    15301 cgagcttagg gcaggtacat ttagctctgc tgaaacactt aaaagtgagc ttacgaactt
    15361 acagccaaaa gaaatcctta tcccctgtga tacggacggg tacgacttta taaatgattt
    15421 atctaatgat atatggaaac ccgttattac aaaaaaagag gagtatctct gggagcttca
    15481 aagttgtgat gaactgctta aaaactattt aggtgttaaa accctgcagg catttaatct
    15541 tgagaacaag ccggactgca ttgtagctct tggagtctta attaactact tgttatctac
    15601 ccagattgac gatatgcctc cgcttaaaaa ccctgtgttc tatgacagga acaacttttt
    15661 acagatagac gggacaacaa tgtccaatct ggaaatcctg agaactatta gaggcgatac
    15721 caaatccggc actctactgt cagttattga tatgacgctt tgtgccatgg gtgcaagact
    15781 tctaagacag tggataaact accctcttac agattcagta aaaataaata aaagattaca
    15841 aagagtggac gttttgtcca aggatccggc tcttaaaaga aatcttaggg caagccttaa
    15901 agaaatatcc gatatagaaa ggataataac tcgcatctcc acaaactcag caaagccaag
    15961 agaccttggt gctattagag attccatagt tttcataaaa gaaattaaaa atgttctaat
    16021 agattctaat attttagaat taggagagtt agagcaaaag ctcgatagcc tgcaagacat
    16081 atatgaaatt attagtgaat ctattgtttc agagccgcca aatacgctta gaacaccgcc
    16141 tgttataaaa gagggtttta ataaggagct tgatagcctt agaagtctaa caaaagatgg
    16201 caagaagtgg atagcagaac ttgaggcaaa agaaaaagaa gcaacaggga taaatttcct
    16261 taagatcggt tataacaagg tctttggata ctacattgag gttaccaagg caaatatcca
    16321 tcttgtaccg gaaagctaca taagaaaaca gacacttgta aatgccgaga gatttatcac
    16381 tcctgatcta aaagagcagg aagaaaagat cgtgggtgca gaagaaaaga tacttgatct
    16441 ggagaggacg ctatttgaac agataagaaa gcaggtagag gcacattctc ataggataag
    16501 aaaaacaagt gcagttatag cagagataga cgtattgtgc ggactatcag aaactgctga
    16561 aaggaattcc tatgttaagc ctgaaataaa taattcagat ataatcgatt tacatgggtc
    16621 aaggcatccc gtcatagaat ctataattac agacgaaaga tttgttccaa acgacattaa
    16681 atttgactgt aaggaaaatc agctcctgct tattaccggc cccaacatgg cggaaaaatc
    16741 gactctaata agacaggctg ctctctgtgt gcttatggca cagattggat catttgtacc
    16801 tgctaaaagt gcatctatag gagtagtgga taagatattc acacgcgtgg gcgcatcgga
    16861 caatctttcc aagggccagt ccacctttat ggtagagatg gttgagacgg cttatatcct
    16921 aagaaatgca acagataaaa gtttggtaat tcttgatgag attggaaggg gaacaagcac
    16981 gtttgacggg atgagtatcg catggtctgt ggcagaattt ctgcacgacc tcggatgcaa
    17041 gacactgttt gcaacccact accatgagct tgccgagctt agtaaggata aggaaagggt
    17101 aaaaaactat aatgtatgcg taaaagagtc tgatgataaa atcgtatttc taagaaagtt
    17161 aatcgaaggt gccgcaagcc acagctacgg aatagaagtt gcaaaacttg caggtatccc
    17221 aggtgaagta ataagctcag caaaaaaagt tctttataat cttgaaaaaa ctcagtctga
    17281 cattaaaagc agtatttcag aagaccagct taagcttttt aacaataaac aggaagataa
    17341 aaaagaaacc gagcttatag atcaattaaa aagccttgat cttgataata tgactcccat
    17401 gcaggctctt tcaaagattg ctgaactaaa agaaaaacta aaagagacgt aaaatttttc
    17461 ttatgaaact acttgagcat atatcaaata tatttaataa gaaaaatgaa ctggataaca
    17521 aatcaagagg agagcttgga gaaaaaaagg catgtaagtt tataaagaaa aatggctacg
    17581 aggtactaga aaaaaattac aggaccaaat ttggtgagat agatattgtc gcttctgaac
    17641 atgacacaat ttgttttatt gaagtaaaag caagatcgaa ttcagattat ggtaacccgg
    17701 aggagtttgt aaccaaaaag aagcaggaaa agctctggaa aacggcttct atatatattg
    17761 ataacaatcc ttcagatctg gaaaattaca ggtttgatgt aattgcaatt gactttgaaa
    17821 gcaataatgt agaaatattt aaaaacgcat tcgagccgga ggtttcattt taactaatag
    17881 taattaatca ccctctttat atcctctatc aatttttcct gatctatatt ctctatctga
    17941 tatgcaacgg gtttatcact gtgagtaacg tctccgtgaa gataaaacgt cttgtcatca
    18001 actatataaa aataagaatt caaaacaatc ctgttttcaa aatctattat tgatagcgct
    18061 cttaaatctc tataatcaaa tcctacatca aaatctgttt ttagatcggg attttgatta
    18121 aaactgcttt tatcggtaag aacacagtac catccttcct gttcaaactg atctcttatt
    18181 tcttcaagca atttatatag tttgtccatc tcgtcctttt agttagaata gttgaatatt
    18241 taatattatt ttacaatact gagaattttc gagtaacatt gtaaatgttt attatactaa
    18301 tccaaagaga atataaagga aaatagatga aagtaatcgt agcaaagcca aggggttttt
    18361 gtgcaggagt ggacagggca atagaaattg tggaacgcgc gcttgagatg tacggcccgc
    18421 caatatatgt gcgtcatgca atagtgcata acaaaagggt agtagaaaaa ctaaaggcca
    18481 aaggtgttgt atttgtagaa gaactagaag agataaagga aaaagattcc cgtgtaatct
    18541 tcagtgcaca cggagtaagt cctgccgtct ggaatgaagc caaggataga agcctgcaat
    18601 ctgttgatgc agtttgtcca cttgtaacta aggtacacag tgaggttaaa cactatgcag
    18661 ataaaggcta ttccattgtt ctaatcggac atagaaacca cgtagaggta atcggtacaa
    18721 gcggtgaagc gcctgaaaaa gttatagtag tagaatcggt agaggaagcc gagaaactag
    18781 aattacccga tcccaataaa gttgcctatg taacacagac aactcttagt gtggacgata
    18841 caagagatat tgtcgaaaca ttaaaggcaa gatttccaaa gattgtagct ccatcaaaac
    18901 tggatatttg ttatgctacc caaaacaggc aggatgctgt gaagcagctt gcaaagcact
    18961 cagatatgat ttttattatg ggctctcctg aaagttccaa ctctaaccgt cttgtagagg
    19021 ttgctaagtc atgcggtgta gaaaaagcat ctttaataga aggaatagag gatttagata
    19081 aagatatgtt ttcagatgat atgactatag gacttagctc aggcgcatca acaccggaga
    19141 ctgtggttca ggaagtaatt tcaaaactca gagaatttgg tgcagagagt gtagaagaac
    19201 ttgacggcaa ggaagagttc acacgatttc cctttgaaga acacttggaa tttaggagtt
    19261 cttataactg agttaataaa taagcgcatc tagaatttct aaaacttcat taaatatttc
    19321 aggtgaagcc ttttcaacaa actttatttt tctgcttata taatcaattg atttaacctg
    19381 atcaaccata ataaaacctg ttagctgtga ctcgctggga acttttacat gaaatggtac
    19441 atttctgtcg gtgttggtaa taggacatgc aattgcaagc ccggtggctt tattgaatag
    19501 gtcgttgctt ataataagcg ctggcctcct gcctttttgt tcgcgacctg attgtggatc
    19561 gaaagttagg gttacgaaat cacctttttt aggtatataa gctgccactt accagacttc
    19621 cttgcctact ggcgctcccc agtcttcttc agaagggtta tagtttttat ctatctttga
    19681 tacgagctgc tcaagtttct tcttgcccct ttttggtttt acagacttaa tgataatctt
    19741 tccgtcttta accgtcatat ccacttcatc accaacttca atcttggcct tctccaaaat
    19801 atatttagat actctgagcc cctggctatt tccccatttt tgaatttttg taaccataac
    19861 aatttcctta aaaaagtata tacatagtat acactgcaat tttattatct caacatatct
    19921 gatgagtata ttagag
//
LOCUS       DBNGSV010000014         4168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_140680, whole genome shotgun sequence.
ACCESSION   DBNGSV010000014 DBNGSV010000000
VERSION     DBNGSV010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_140680"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="AC159U_00735"
     CDS             <1..2036
                     /locus_tag="AC159U_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3335614.1"
                     /translation="IGSIDTNSDEKILFTTNTILADIATAQNLLDFNGSLSRIDLDLT
                     SYPQEQIGQIKHFLTINYPQVSLIKADSLVEQSSRLTKSFELNLTALSLITLLIAFFL
                     IYNSFNYSLVIRRPVFSVLRSIGVTSNEIFYTILTEAFIIGILSSVLGIIVGIFLGKL
                     SVLLVSNTLSSIYSVFGQSGLSLSPITIIKAIILGITAVLSAAIIPSLESRFIKPVSL
                     MSESYFNKNFIRLIPYLFTAGIFLICSGLYISAFLSSNITLTFAGLFIIIIGYTLLLP
                     ALMLVLAKILNRYAKSLFGKIYLIPCRNYLGSIDKNFVATASLTVAVAVFLSMTVLIE
                     SFRSTLTAWLEDTFPSDVFISAVYRPEENNGLNFNLINKITSLEGTHKIGYSRKIDLL
                     TEDYGRLNLIALSENPPIKPQIISGLINDFKELNNTYDKAVLISESFSNRNSWQLKDG
                     YLFLPTMKGYEKFLIAGVFSDFATQTGTLIIGYESFRKLWDDDKLTSISLNISKGFEL
                     NSVLGNIRANISDEQEFRLSTNKDLKNSAHKIFNQTFVITNAMRVIAFAVAFFGILGA
                     LLALELSRKKEIGVLRALGVKVLEIRKIIFSETAIIGLISGVISIPLGLTMAYLLVNV
                     INFRSFGWTIDFLIKPGYLAEAVLISFIAAMLAGIYPSIIFSKINISEAIREE"
     gene            2045..3178
                     /locus_tag="AC159U_00740"
     CDS             2045..3178
                     /locus_tag="AC159U_00740"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR3335615.1"
                     /translation="MKSRIAYSVVGFFVLLITLALFNQNVNLFDESDNPTIDISAVLS
                     GKEKNKGFKRAVRPIQFSFPKDHGTHPDYQTEWWYFTGNLKDEENNNFGYQLTFFRRA
                     LTPDQPVDGSSWRASSIYFAHFAVTDISNKNYHSFEKWSRATPRLAGSDNKDLHVWID
                     DWMVNSDDNNNYILSSNSENISVSLVLKESKKIVLNGNKGLSQKSDELGNASYYYSHT
                     RLKTTGTITIDDKKHKVDGLSWFDHEWSTSALGDLQSGWDWFSLQLSNNTEIMIYMLR
                     LKDGTVDPVSSGTYVDKSGDTVHLKSTDFIVETKEHWKSPGTNIKYPSKWTVMIPKYN
                     IELDVQPVLNNQEFNHSFTYWEGAVKVKGDNISGLGYVELTGY"
     gene            3250..3681
                     /gene="mce"
                     /locus_tag="AC159U_00745"
     CDS             3250..3681
                     /gene="mce"
                     /locus_tag="AC159U_00745"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3335616.1"
                     /translation="MPQEKAKNINHIALAVTNLSEAENLYVKALGLEVKHREVIKDQG
                     VSTSMLKSVEGQTSIELLEPLDDNSPISKFLDKRGEGIHHICFEVDDIEQSLQHLKDL
                     DLELIDESPRIGAYGAKVAFVHPKSMNGVLIELAEFSKENS"
     gene            complement(3695..3937)
                     /gene="tatA"
                     /locus_tag="AC159U_00750"
     CDS             complement(3695..3937)
                     /gene="tatA"
                     /locus_tag="AC159U_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3335617.1"
                     /translation="MGNIGGWELIIILVILLLIFGPSRLGDLGTALGKGIKGFKRSMK
                     EPDEIDVTPNKDDSDKIEDTDLEKAPKKNEKESEVK"
BASE COUNT         1348 a          710 c          776 g         1334 t
ORIGIN      
        1 taatagggag tatagataca aacagcgatg aaaagatttt atttaccaca aacacaatat
       61 tagctgatat tgcaacagcc cagaacctat tagattttaa tggaagttta tcaaggattg
      121 atttggatct aacaagttat ccgcaagaac aaatcggcca gataaaacat tttctcacaa
      181 taaactatcc acaggtctct cttattaaag cagactcctt ggtagagcaa agcagcagac
      241 ttaccaagtc atttgagctc aatctaacag cccttagttt aattactctg cttattgcgt
      301 tttttttgat ttataattct tttaattact cacttgtaat aagaaggccg gtattttcgg
      361 tactaagatc gatcggggta acatcaaatg agatattcta tactattctc accgaagcat
      421 ttataattgg tattttgtcc tcagtgctag gaattatagt tggaatattt cttggaaaac
      481 tttctgttct tcttgtatca aatacactat ccagtattta ttcggtgttt ggccagtctg
      541 gattgagttt atctcctata accataatta aggctattat tttagggata actgctgtat
      601 tgtcggctgc aatcatacct tctttggaat ccagatttat taaaccggtc agtcttatga
      661 gtgagtcata tttcaacaag aactttatac gtctcatccc ttatttgttt actgccggta
      721 tttttcttat ctgttcagga ctctatatat cggcattttt atcatcaaac attacactta
      781 catttgcagg gctatttata attatcatag gatatactct tctgctacca gcattaatgc
      841 tagtgctagc aaagatttta aacagatatg caaaatcatt atttgggaaa atatatctta
      901 tcccatgcag aaactacctg ggctcaattg ataagaactt tgttgccacg gcgtcgctta
      961 ctgtggcggt agctgttttt ttaagcatga cagttctaat tgaaagtttc agatctactc
     1021 taacggcatg gctggaagac acctttccat cagatgtatt tatatcggct gtctacaggc
     1081 ctgaagaaaa taacggtttg aattttaatc tcataaataa aattacatca ttggaaggta
     1141 ctcacaagat tggatattca agaaaaatcg atcttcttac tgaagactac ggcaggttaa
     1201 atctgattgc tcttagtgaa aatccaccca taaaacctca aattatctca ggtctgataa
     1261 atgatttcaa agaattaaat aatacttacg ataaagctgt attaatatca gagagtttta
     1321 gtaacagaaa ttcttggcaa cttaaagatg gatatctctt tttacctaca atgaagggtt
     1381 atgaaaaatt tctgatagca ggcgtatttt cagactttgc aacgcaaaca ggcacattaa
     1441 taataggtta tgaatcgttt cgcaaacttt gggatgatga taagctaacc tctatttccc
     1501 ttaatatatc taagggattt gaattaaaca gtgtacttgg gaatataaga gcaaatatct
     1561 cagatgagca agaatttagg ttaagtacta ataaagacct taagaactca gcgcataaaa
     1621 tatttaatca gacttttgta attacaaacg ctatgagggt gattgctttt gcagtggcat
     1681 tttttggcat actcggagca ctccttgcac tcgaactgtc gaggaaaaaa gagattgggg
     1741 tgcttagagc tttgggtgta aaggtcttag agattagaaa gataatattt tctgaaacag
     1801 caattattgg cctcatatca ggggtaatat caatcccgct tggattaact atggcttacc
     1861 tgcttgtaaa cgtaataaat tttaggtcat ttggatggac tatagatttt ctaattaagc
     1921 ctggttattt agccgaggca gtcttaatat catttatagc ggcaatgctt gcgggtattt
     1981 atccctccat aattttctca aaaataaata tttcagaggc tataagagaa gagtgattaa
     2041 acatatgaaa tccagaatag cttattcggt agttggtttt ttcgtattgc taattaccct
     2101 tgccttattc aatcaaaatg taaatttatt tgatgaatct gataacccta caattgatat
     2161 atcggctgtg ttatctggta aggaaaaaaa caaaggattc aaaagagcag tacggccaat
     2221 acagttttcc tttccaaaag atcacgggac tcatcctgat tatcaaacgg agtggtggta
     2281 ttttacgggt aatttaaaag acgaagaaaa taacaatttt gggtatcagt taacattttt
     2341 caggcgggca ctcacacctg atcagcctgt agatgggtca tcatggagag caagctcaat
     2401 atattttgct cattttgcag ttacagatat aagtaataaa aactatcatt cctttgaaaa
     2461 gtggagcagg gctacaccca ggcttgcagg ttctgataat aaagatctgc atgtgtggat
     2521 tgatgactgg atggttaatt cggatgataa taacaactat attctcagtt caaatagtga
     2581 gaacatatca gtttcacttg tactaaaaga gtctaaaaaa atagtcctaa atggaaataa
     2641 gggactgagt caaaaaagtg atgaactggg caatgcatca tattactatt cacatacaag
     2701 acttaaaacc accggcacaa ttacaataga tgataagaaa cataaagtgg atggcctcag
     2761 ctggtttgat catgaatgga gcacaagtgc tctgggagat ttacaatcgg gctgggattg
     2821 gttttccctt cagctaagta ataatacaga aattatgatt tatatgctgc ggttaaaaga
     2881 cgggacggtt gatcctgtat cttctggaac ctacgtggat aaatcgggtg atacagtaca
     2941 tctaaagagc actgatttta ttgtagagac aaaagagcat tggaaaagtc ccgggacaaa
     3001 catcaagtat ccttcaaaat ggactgtcat gattcctaaa tacaatatag agttagatgt
     3061 gcagcccgtt ttaaacaacc aggagttcaa ccactccttt acctattggg agggggcagt
     3121 gaaagtaaaa ggggataaca tcagtgggct ggggtatgta gaattaactg gttactgatt
     3181 aatttatatt ctcttttagg atgttgaggg tatcattcta tttttaattt gagataaacg
     3241 gagaccttaa tgcctcagga aaaagccaag aacataaatc atatagcgct tgcagtcaca
     3301 aatttatcag aagcagaaaa cttatatgta aaagcccttg ggcttgaagt taaacaccgc
     3361 gaagtaataa aagatcaggg agtaagtact tctatgctaa aatctgtaga gggacaaact
     3421 tcgattgaac tgctggagcc tttggatgat aattctccaa tctcaaaatt cctagataaa
     3481 aggggagaag ggattcacca tatatgtttt gaggttgatg atattgagca aagtttgcag
     3541 cacttaaaag acctggatct tgagctaatt gatgaaagtc ccagaatagg cgcttatgga
     3601 gccaaagtag cttttgttca tccaaagtct atgaacgggg tgttaatcga gcttgctgaa
     3661 ttttcaaaag aaaatagtta gataaataat cctattattt aacctctgat tccttttcat
     3721 ttttttttgg ggctttttcc aggtctgtat cttctatttt atcgctatca tctttattag
     3781 gagtaacatc tatttcatcg ggttctttca tcgatctttt aaaacctttg atcccctttc
     3841 caagtgcagt acccaaatct ccaagcctgc ttggcccgaa tataagtagt aaaatcacaa
     3901 gaattattat taattcccag ccgccaatat tacccatgat gcttttctcc tataaatcac
     3961 aaatctaaag taagtatata gttttattat acctaatcag tataaattga attatgcctt
     4021 tagtaaaaaa ttatagaaaa gtaattaggt gactaaaacc tgctggcttg tttatatacc
     4081 tgctcaagtt ctttggtttt actcagaact tttacaccca tttctgcctg agcgccttct
     4141 tttggagggg aattccaaac aacttcac
//
LOCUS       DBNGSV010000015        10629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1110431, whole genome shotgun sequence.
ACCESSION   DBNGSV010000015 DBNGSV010000000
VERSION     DBNGSV010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1110431"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..630
                     /gene="rimI"
                     /locus_tag="AC159U_00755"
     CDS             154..630
                     /gene="rimI"
                     /locus_tag="AC159U_00755"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3335618.1"
                     /translation="MTVGSSYKIEPMNKDHISQIMEIEKVSFPTPWHKRIFELEIGKP
                     RSFHSVFKLEDKVVGYLISWMLYDEIHILNVAVHPEHRRGGIGRKLIEYTIAHFRPKG
                     AMTVILEVRTSNIAAQNLYEKMGFEVLRTRKKYYSDTGEDALVMMLDISGDYDNDW"
     gene            complement(640..2250)
                     /locus_tag="AC159U_00760"
     CDS             complement(640..2250)
                     /locus_tag="AC159U_00760"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3335619.1"
                     /translation="MSKKPKERRESFTTPSGIEINRLYGPEDTKNIDYESEIGQPGSY
                     PFTRGVQSTMYRGRLWTMRQYAGFGTAEETNERFKYLLDQGQTGLSVAFDLPTQMGYD
                     SDDAISRGEVGKVGVAIDSLEDMEILFSGIPLSDVSTSMTINSTASMLLAMYMVAAEK
                     QGTGPEQIKGTVQNDLLKEFIARGTYVFPPGPSVKITIDILEYCRDHIPSWNPISVSG
                     YHIREAGSSATQESAFMLADAIEYLENARQRGLDIEKIAERVSFFFAVHNNFLEEVAK
                     FRATRRLWAKILKDRFNVTNERAQKFRVHSQTSGVTLTAQQPKNNIMRVTMQALASVL
                     GGTQSLHTNSYDEALALPSEEAATIALRTQQLIAHESGVADTIDPLAGSYAVESLTNE
                     IEDKVLEYITKIDSKGGMIKCIEDGYINQEIEEASYNYQLEVEKKERIIVGVNDYVAD
                     NEQPVDILKINPEIESIKKKKLKDLREKRDNKKVANGLDELENAARNSENLMPYIVQA
                     VRDYATIGEMTRALEKVYSRFGRQSQAV"
     gene            complement(2277..3278)
                     /locus_tag="AC159U_00765"
     CDS             complement(2277..3278)
                     /locus_tag="AC159U_00765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3335620.1"
                     /translation="MGLTDILSIISDDLNRVEQEIDKNIQSPAPLVYEISKYLLEGGG
                     KRIRPAILLLCSGSLGLKNGALQDGWKKIASAAAIELIHTASLLHDDVVDEASVRRGK
                     ESSNVVWGNKPTVLVGDFLLSKALGLIQSCGNLDLIKAVTDASAKLAEGQVLEVMSSA
                     SMIETTEDVCFGIIENKTASLIESSARVGALLATDSDETNKVDAVGNYGLNLGIAFQL
                     VDDDLDYSSTEQEFGKQVGQDLVEGKMTLPLYYSIQDATKTEQEKVSRILGKDIFDEQ
                     ELSFIFEIVSKYKGVDKTKDSAKSFVNKAKESLTYLPVNEFNKSLDMLADFVVERTN"
     gene            3445..5685
                     /locus_tag="AC159U_00770"
     CDS             3445..5685
                     /locus_tag="AC159U_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3335621.1"
                     /translation="MKNHLIKKLLSNFGSAACMVLSAVFAVFVVINPTNVKGAETNKM
                     GKQNRLSKEKSPYLLQHADNPVDWYPWGKEAFDKAKKEDKPILLSIGYSTCHWCHVME
                     HESFEDEEVAKLMNEVFISVKVDREERPDIDNVYMSVCHMLSQGSCGWPLNIIMTPDK
                     KPFFAATYIPKHNRFGRAGMMDLVPKVGEMWKKQREDLVKSADKITFALKQNSSTKIL
                     DSTVSYEVSILDTAFSQLEQGFDTIHGGFGRAPKFPSPHNNLFLLRYWKRTGNEKALA
                     IVEKTLKEMRMGGIFDHIGYGFHRYSTDPEWLVPHFEKMLYDQAMIAMAYVETYQATK
                     DEFYKETAKEIFEYVLRDMTSDNGGFYSAEDADSEGVEGKFYVWEKEELIKVLGKEQG
                     ELFSQIFEATDKGNFADEASGKIEGDNILHLKKTISQHAVDIKQSEKELLGKLESARE
                     KLFKIREKRIHPYKDDKILTDWNGLMIAALAKAARAFDDDKYTKAAQMSAEFILSKMK
                     TPEGGLLHRYRLGDAAIDATIDDYAFMIWGLTELYETTFDIDYLKEAVELNKFALDKF
                     WDDKGWGFYFTPDGGEQLLVRPKEIYDGAIPSANSVFLLNLLKLAKLTADSDLEDRAH
                     ELSQAFFEDVSRRPAGFTQFMAGLDFALGPSFEVVLVGTPGKADTNKMIDSLRNEYFP
                     SKVVLFKNVADDTEQLNKIAPYSKSHIQIKDKATAYVCKNYICNLPTSDSEKMMSLLK
                     NYKEKK"
     gene            5755..5922
                     /locus_tag="AC159U_00775"
     CDS             5755..5922
                     /locus_tag="AC159U_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335622.1"
                     /translation="MARESRRKVKDLVEMLENPDDSGYVGGDYEDMMEELERELMLEE
                     EDDEENEDEDY"
     gene            complement(5919..7820)
                     /gene="mnmG"
                     /locus_tag="AC159U_00780"
     CDS             complement(5919..7820)
                     /gene="mnmG"
                     /locus_tag="AC159U_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR3335623.1"
                     /translation="MMSIKYDKIHEVIVIGAGHAGCEAALASARMGCKTLVLTANIDT
                     IGLMSCNPSIGGVGKGHLVKEIDALGGEMGKAADATAIQFRRLNTRKGPAVQATRTQA
                     DRSLYRLYMRKTMESEPNMDIKQRMVEGFIVEGNKIAGVRTNIGEDFYAGCVVVTPGT
                     FPNGLIHIGKTQIVSGRAGEQSATEISNAFEQLGFNVGRLKTGTPPRLDGRTIDWQRT
                     EIQPGDENPVPFSLSTKSIDSKQLPCHITYTNEKTHQVIRDNLDKSPLYSGSIKGVGP
                     RYCPSIEDKIVKFPDRERHQIFLEPEGYDTYEIYPNGISTSLPLEVQYQIVRTIEGLE
                     NVEIMRPGYAVEYDFCDPTQLKNTFETKLVENLFFAGQINGTTGYEEAGAQGLIAGIN
                     AALKIKGEPELTLDRSEAYIGILIDDLITKGVDEPYRMFTSRAEHRLILREDNADIRL
                     REIGYKIGLVSEEDFAKFEAKQKRVEEEMTRLHGKKIYPTPEVNNILEKFNSSALKKP
                     LSLREVLKRPEISYNELSFIDINSVKMTNSDIRSQIEMEIKYEGYIKREKEQALKFRK
                     LENVTIPHDFTYENIPGLSNELVQKLSRVKPNSIGQACRISGITPAAISVLMVYLKKS
                     ESEQAARMD"
     gene            7898..8926
                     /locus_tag="AC159U_00785"
     CDS             7898..8926
                     /locus_tag="AC159U_00785"
                     /inference="COORDINATES: protein motif:HMM:NF045247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPL family radical SAM protein"
                     /protein_id="HIR3335624.1"
                     /translation="MNKNMQKPDRLYIEKGVEDFELTKKILSRLGPIDTVYVEDYKKI
                     GFDKNFTDRANEDKNSLALGQKKGEVLKSIGRMDDGQYYLFHEMDCKYDCEYCYLQYY
                     FQTKVPVIFVNRSEVLDRMKEAMKLHSTTYFHVGEIADSLAFDNLTDFSLDLAYLAKN
                     NENCTIEFRTKSTNIENLLSIENPTPNIIPSWTMSPQIVSETLEHKTPKFSERLEAGK
                     ACQENGYTVGVRIDPIIIYEDWENGYSEMINQIMTTLDPKCIDYITLGSIKIHKILVD
                     VIRMRFPKSPLLLGEIFPGEDGKYRYMKFQRVDVYKQILSWIRDYDSDIEVELSLESE
                     DIRELVFG"
     gene            complement(8954..10339)
                     /locus_tag="AC159U_00790"
     CDS             complement(8954..10339)
                     /locus_tag="AC159U_00790"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR3335625.1"
                     /translation="MVKEKPDADNSSQRGNNKIATSKQKSKKRIKAPKGKLGVLLPGI
                     GGAVSTTFVAGVEAIKAGIGKPIGSLTQLGSIRLGKRTEKQSPLIKDLVPLYDIEDLV
                     FCGWDPYPENCYDAALNSGVLEQELLAQIKEPLQNISPNKAVFERKFVKNLSGRHVKK
                     AKTKMDLANELIKDIETFKKENNLDRLVMVWCASTEVYLEKSDVHKSIKKFEKGLKEN
                     NPHIAPSMIYAYAAIKTGTPYANGAPNLSADIPALEELAKKNKVPICGKDFKTGQTLM
                     KTILAPGLKSRLLGLNGWFSTNILGNRDGEVLDDPYSFKTKEASKLSVLEYIFQPDLY
                     PELYSDYYHKVRINYYPPRGDEKEAWDNIDIFGWLGYPMQIKVNFLCRDSILAAPIVL
                     DLVLFLDLAQRAGMSGIQEWLSFYFKSPMVGKRLYPEHDVFIQQMKLKNTLRYIAGEE
                     LITHLGQDYDD"
     gene            complement(10293..>10629)
                     /gene="rpsI"
                     /locus_tag="AC159U_00795"
     CDS             complement(10293..>10629)
                     /gene="rpsI"
                     /locus_tag="AC159U_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3335626.1"
                     /translation="LRPGDGTITVNNREVNTHFPRKTWQLKVLKPLELTGSTNKYNVM
                     MHVKGGGLTGQADACAHGLSRALVKADEANNKKPLKAADLLTRDPRMVESKKYGKRKA
                     RRGQQFSKR"
BASE COUNT         3112 a         2050 c         2123 g         3344 t
ORIGIN      
        1 gcacttccaa tacaaacatt tgtattagct tgcttatctt actatttgat agttttgtat
       61 tcttaagtaa cttcttttgc atagtttcag cttagtatac tttgctgtcc tatgcgagct
      121 ccaaaaacaa agcagatgta atattttcgc gttatgaccg ttggtagcag ctataaaatc
      181 gagcctatga acaaagatca tatctctcag atcatggaga tagaaaaagt cagttttcct
      241 accccatggc acaagaggat ttttgagctg gaaatcggga agccgcgctc atttcactcg
      301 gtctttaaat tagaggataa agtagtgggg taccttattt catggatgct ttatgatgaa
      361 atacatattc taaacgttgc tgtacatcct gagcatagac gaggcggtat aggcaggaag
      421 cttatcgaat acactattgc tcactttaga ccaaaggggg caatgacagt tattttagaa
      481 gtgagaacaa gtaacatagc tgcacaaaat ctttatgaaa aaatgggctt tgaggttctg
      541 agaaccagaa aaaaatatta ctcagacaca ggagaagacg cgcttgttat gatgcttgat
      601 ataagcgggg attatgataa tgattggtaa accaataggt tacacagcct gactttgcct
      661 gccaaagcga gaatagacct tttctaatgc tcttgtcatc tctccgattg tagcatagtc
      721 cctgacagct tgaactatat agggcataag gttttcgctg tttcttgccg cattttcaag
      781 ctcatcgaga ccatttgcaa ctttcttatt atctcttttt tcccttaggt cttttaattt
      841 ctttttctta attgattcta tttcaggatt aattttaaga atatcaaccg gttgctcatt
      901 atctgcaacg tagtcattaa cgcctacaat aattctttcc ttcttttcga cctcaagttg
      961 atagttataa gaagcctctt ctatttcctg attgatatat ccgtcttcaa tgcactttat
     1021 catcccgccc tttgaatcaa tctttgtaat gtattctaaa actttatctt caatttcatt
     1081 tgtaagtgat tcaactgcat aagagcctgc cagagggtct attgtgtctg caacaccgct
     1141 ttcatgtgca atgagctgtt gggttctaag agcaatagtg gccgcttctt ctgatggcag
     1201 agcaagggct tcatcataag aatttgtgtg cagtgactgt gtacctccaa gaacagaagc
     1261 aagagcctgc atcgtaactc tcattatatt gttttttggc tgctgtgccg tgagtgtgac
     1321 gcctgatgtt tgagaatgca ccctgaactt ttgtgctcgc tcgtttgtta cattaaatct
     1381 gtctttaagg atttttgccc atagccttct agtggctcgg aattttgcaa cctcttctaa
     1441 aaagttatta tgaacagcaa agaaaaaaga aaccctttcg gctatttttt caatatccag
     1501 gcctctctgc ctggcatttt caagatattc tattgcatct gcaagcataa aggcagattc
     1561 ctgtgttgct gaagacccgg cttctctgat atgataacca ctcacactaa tgggattcca
     1621 gcttggtata tgatcccggc agtactcaag gatgtctatt gtgattttca cagacgggcc
     1681 tggaggaaaa acataagtcc ctctggcaat aaactcttta agcagatcgt tttgtacagt
     1741 gcctttaatc tgctccgggc ctgttccctg tttttctgct gcaaccatat acatcgcaag
     1801 gagcattgat gctgtagaat ttattgtcat tgatgtgctt acatctgaga gcggaatgcc
     1861 ggaaaaaagg atttccatat cctcaagtga atctattgct acccctacct ttccaacttc
     1921 tcccctactt atagcatcgt ctgagtcata tcccatctga gtgggaagat caaatgctac
     1981 gctaagcccc gtctgtccct gatccagaag atatttaaac ctttcattag tttcttcagc
     2041 agtaccaaag ccagcgtact ggcgcattgt ccacagcctt ccgcggtaca tggtagactg
     2101 aactccacga gtaaacggat agcttccggg ctgtcctatc tcgctttcat agtctatgtt
     2161 tttagtgtct tcaggaccat agagtctgtt aatctctatt cctgagggtg tggtaaatga
     2221 ttctcttctt tcttttggct ttttactcat tttaataatt gaaggatcat tagaagttag
     2281 tttgttctct ctacaacaaa atcagcaagc atatccaggg atttattaaa ttcattgaca
     2341 ggcagataag ttaatgactc ttttgcttta ttaacaaaag attttgcaga gtcctttgtt
     2401 ttatctacac ctttgtattt gcttacaatt tcaaagataa aagatagttc ctgttcatca
     2461 aaaatgtctt taccaagaat tctgcttacc ttttcctgtt ctgttttagt tgcgtcctga
     2521 atggaataat agagcggtag tgtcattttt ccttcaacaa gatcctgccc tacctgcttt
     2581 ccaaactcct gctcagtgga gctatagtca agatcgtcgt caactaactg aaatgctata
     2641 ccaaggttga gtccgtaatt tcctacagca tctaccttat ttgtttcatc tgaatccgtt
     2701 gcaagaagcg ctcctactct tgcgctgctt tcaataagag aagcggtctt gttttctatt
     2761 attccaaaac aaacatcctc agtagtctca atcatgcttg cagagctcat gacttccaat
     2821 acctgacctt ctgcaagctt ggccgaagca tctgtaactg ctttgatgag gtccaaattt
     2881 ccgcaggatt gaattaaccc cagagccttt gataacagaa aatcacccac aagaactgta
     2941 ggtttgttac cccataccac attagatgat tccttgcccc ttctgactga agcttcatca
     3001 actacgtcat catgaagaag ggatgcagta tgaattaact caatagcagc agcagaggca
     3061 atctttttcc atccgtcctg aagcgctcca tttttaagtc ccagggagcc gctgcaaaga
     3121 agaagaattg caggccgtat tcttttgcca ccgccttcga gcaaatactt tgatatttca
     3181 taaacaagag gagcagggga ctgaatattt ttgtctatct cttgctctac cctattcaaa
     3241 tcgtctgaaa ttattgataa tatgtctgta agtcccatca gtaagcctta aattatctga
     3301 actaacctat atagtcaaag tttaggataa agaatacagt ttccacacaa ataactaaaa
     3361 tatttattaa aatatactaa tatacattat acttaaaagg gtttgataaa ctaaccggta
     3421 cgtaagcagg ttctgtaatc tataatgaaa aaccatttaa taaaaaaatt gttaagtaac
     3481 tttggatcag ctgcgtgcat ggttctatct gctgtatttg ctgtatttgt tgtgataaat
     3541 ccaactaatg ttaaaggagc tgaaacaaac aaaatgggaa aacaaaacag gcttagtaaa
     3601 gaaaagagtc catatctgct ccagcacgca gacaaccccg tggactggta cccatggggg
     3661 aaggaggcat ttgataaagc taaaaaagaa gacaaaccca ttttgctctc tattggatat
     3721 tccacatgcc actggtgcca tgtgatggag catgagtctt ttgaagacga agaagtagca
     3781 aagcttatga atgaagtatt tatttctgta aaagtagacc gggaggaacg tcctgatatt
     3841 gataacgtat atatgagtgt ttgccatatg ctctcacagg gaagctgcgg ctggccgcta
     3901 aatattataa tgacaccaga taaaaaacct ttttttgcag ctacgtatat tccaaaacat
     3961 aacaggtttg gaagggcggg tatgatggat ttagtaccca aagtaggcga aatgtggaaa
     4021 aagcaaagag aagatttagt aaaatcagcc gacaaaatta catttgcact aaaacagaat
     4081 tccagcacaa aaattctgga ttctaccgtg agttatgaag tttcaattct ggatactgca
     4141 ttttcacagt tggagcaggg gtttgataca attcatggag ggtttggcag agctccaaaa
     4201 tttccatcac cccacaacaa cctgtttttg ctaagatact ggaagagaac gggaaacgaa
     4261 aaagcacttg caatagtaga aaaaacgcta aaggaaatga gaatgggcgg gatttttgat
     4321 catataggct acggatttca ccgctactca actgacccgg agtggcttgt acctcatttt
     4381 gaaaagatgc tttacgatca ggctatgatt gctatggctt atgtagaaac ctaccaggca
     4441 acaaaggatg aattctataa agaaacagct aaggagatat tcgaatatgt gttaagggat
     4501 atgacctctg acaacggagg gttttattcg gcagaagatg cggatagcga aggggtagaa
     4561 ggaaaatttt atgtctggga aaaagaggag cttataaagg ttttaggaaa ggagcagggg
     4621 gagctttttt ctcaaatatt tgaagcaaca gataaaggta attttgcaga tgaagcaagc
     4681 ggcaaaattg aaggagataa tatccttcat cttaaaaaaa ctattagtca gcatgctgta
     4741 gatataaaac agtccgaaaa agaacttctc ggaaaactcg aaagtgcaag agaaaagctt
     4801 tttaaaataa gagaaaaaag aatacatcca tataaagatg ataaaatcct tacagactgg
     4861 aacgggctta tgattgcggc tctagcaaag gcagcaaggg cttttgatga tgataaatat
     4921 actaaagccg cgcagatgtc tgcagaattt attctatcta aaatgaaaac tcccgaaggg
     4981 ggattacttc acagatacag gcttggagac gcagcgatag atgctacgat agatgattat
     5041 gcgtttatga tttggggttt aactgagctc tatgaaacta catttgatat tgattattta
     5101 aaagaagcgg tggagcttaa caagtttgcc ctggataaat tttgggatga taagggttgg
     5161 ggtttttatt ttactcctga tggtggagag cagcttttag ttaggcctaa agagatctat
     5221 gatggcgcaa taccatcagc aaactctgtg tttttactaa atctgttaaa gttagccaaa
     5281 ctcactgcag attcggatct tgaagataga gctcatgaac tctcacaggc attttttgaa
     5341 gatgtttcaa gacgccctgc agggtttact cagtttatgg caggtcttga ttttgctctc
     5401 ggaccttctt ttgaggtagt actggtcgga actcccggta aagctgatac aaataaaatg
     5461 attgattcac tcagaaatga gtatttcccg agcaaggttg tattatttaa aaatgtagct
     5521 gatgataccg agcagcttaa taaaattgca ccgtatagta aaagtcatat ccagattaaa
     5581 gacaaagcca cagcttatgt ctgcaaaaac tatatctgta atcttcccac gagtgattca
     5641 gaaaagatga tgtcattatt aaaaaattat aaagaaaaaa aataattact tgaaattaaa
     5701 aataactaat attatatttt tggtaatttt taaattcact tatcaggagg ccctatggca
     5761 agggagagtc gtaggaaagt aaaagactta gttgagatgt tagagaatcc tgatgattcc
     5821 ggatacgtcg gtggagacta cgaagacatg atggaggagc ttgaaagaga gctcatgctg
     5881 gaagaggaag atgacgagga aaatgaggat gaggattatt aatccatcct agccgcttgt
     5941 tcactctcag atttttttag gtaaaccata agtacagaaa tagctgcagg agttatgcct
     6001 gatattctac aggcctgccc aatggaattt ggttttactc tggagagttt ttgtacaagt
     6061 tcattggaaa gcccgggaat attttcatac gtaaagtcat ggggtattgt gacgttttcc
     6121 aactttctaa atttaagagc ctgttctttt tctcttttta tgtagccttc atacttaatt
     6181 tccatctcta tctgtgatct gatatcagaa ttagtcatct taacgctatt aatatcgata
     6241 aaagaaagtt cattatatga aatctctggt ctttttaaaa cttctcttaa gctaagaggt
     6301 tttttaagag cagatgaatt aaatttttca agaatattat taacttcagg ggtaggataa
     6361 attttcttac catgaagcct ggtcatttct tcttctaccc ttttttgttt ggcctcaaac
     6421 tttgcaaaat cttcttcact tacaagacca atcttataac ctatttctct cagtcttata
     6481 tctgcattat cttctctcaa tataagtctg tgctctgccc tggaagtgaa cattctgtac
     6541 ggttcatcca caccctttgt gataaggtca tcaattagta ttccaatata tgcctcagag
     6601 cggtcaagag taagttcagg ttcacctttt atttttagtg ctgcattaat accggcaata
     6661 agcccctgag cgccggcttc ttcataaccg gttgtgccgt ttatctgtcc ggcaaagaaa
     6721 agattttcta caagcttagt ttcaaatgtg ttttttagct gggtaggatc gcaaaaatca
     6781 tactctaccg catatccggg tctcatgatt tctacatttt caagcccctc gattgttctt
     6841 acgatttgat attggacttc taacggcaga cttgttgaga ttccgtttgg ataaatctca
     6901 taagtgtcat atccctcggg ctctaagaat atctgatgcc tttctctgtc gggaaatttt
     6961 acaatcttat cttcaataga tgggcagtac ctggggccca cacctttaat tgagccagag
     7021 tacagaggag atttatccag attgtctctt attacctgat gagtcttttc gtttgtataa
     7081 gtgatatgac agggaagctg tttactgtct attgattttg tagagagtga aaaaggcacg
     7141 gggttttcat cgcctggctg tatctccgtt ctctgccaat caattgtcct tccgtcaagc
     7201 cttggaggtg ttccagtctt gagccttcct acattaaacc caagctgttc aaaagcattt
     7261 gaaatttctg ttgcagattg ctctccggct ctgcctgaaa ctatctgggt ttttcctata
     7321 tgaataagtc cattaggaaa agttccgggg gttacaacaa cacagcctgc ataaaagtct
     7381 tcaccaatat tagttcttac accggcaatc ttgttccctt ctacgataaa cccctcaacc
     7441 atgcgctgct ttatgtccat attaggctca gactccatgg tttttctcat gtagagtctg
     7501 tagaggcttc tgtcagcctg ggttcttgta gcttgtacag cagggccttt tctggtgtta
     7561 agtcttctga actgaatagc tgtggcatct gctgcttttc ccatctcgcc gccaagggca
     7621 tcaatttctt ttacaaggtg ccctttacca acgccgccta tagaagggtt acaggacata
     7681 agcccaatgg tgtcaatgtt tgccgtaagc acgagcgttt tacatcccat acgagccgaa
     7741 gcaagcgcag cttcacatcc cgcgtgccct gcgccaatta ctataacctc atgaatctta
     7801 tcgtatttaa tagacataat gtaaacaacc tataataatg ccgatatttt acgagtcaaa
     7861 tatggtattt atgtggtagt ataaaacatt ctgaactttg aataagaata tgcaaaaacc
     7921 agacaggctt tatatagaaa agggtgtaga agattttgag ctaacaaaga aaatattaag
     7981 cagactaggt ccaatcgata ctgtgtatgt agaggactat aagaagattg ggtttgataa
     8041 gaactttaca gatagggcta atgaggataa gaacagcctt gctctggggc agaagaaggg
     8101 agaggtatta aagagtatag gaaggatgga cgatgggcag tattatctct ttcatgagat
     8161 ggactgtaaa tatgactgcg agtattgtta tcttcaatac tacttccaga ctaaagttcc
     8221 cgtgatattt gtaaaccgct ctgaagttct ggacaggatg aaagaagcta tgaagcttca
     8281 ttctactact tattttcatg ttggcgagat tgcagactca cttgcctttg acaaccttac
     8341 tgatttttct ttagacttgg cttatctcgc aaaaaataat gaaaactgca caatcgagtt
     8401 tcggacaaaa agtacaaaca tagaaaactt attatcaata gaaaacccaa caccaaatat
     8461 tatcccgtca tggacaatga gcccgcagat agtttcggaa acactggaac ataaaacacc
     8521 aaagttttct gagaggttag aagctggaaa ggcatgccaa gaaaatggtt atacagtggg
     8581 tgtacgaatc gatccgatca ttatatatga ggattgggaa aatggatatt cggaaatgat
     8641 aaaccagata atgaccacac tcgatccaaa atgcattgac tatataactc ttggttctat
     8701 aaagatccac aagattcttg tagatgtaat aagaatgaga tttccaaaaa gtccactatt
     8761 gcttggagaa attttccccg gtgaggatgg aaagtacaga tacatgaaat tccagagggt
     8821 tgatgtatat aaacaaatat tgtcatggat tagagattat gacagtgata ttgaggtcga
     8881 attatctctt gaatcggagg atattcggga gttggttttt ggttaattac ttactcaaaa
     8941 atattcagaa tacttaatcg tcgtaatcct gaccaagatg ggtaatcagt tcttctccgg
     9001 caatatatct tagagtgttc ttaagcttca tttgctggat aaatacatcg tgttctggat
     9061 agaggcgttt gcctaccatt ggtgatttga agtaaaaaga gagccattcc tgaattcctg
     9121 acataccagc tctttgtgcc aagtctaaaa atagtaccag atcaagaacg atcggtgcgg
     9181 ccaggatgct gtctctgcaa agaaagttaa ctttaatctg catcggatac ccaagccatc
     9241 cgaagatgtc tatattatcc caagcctctt tttcatcccc tcttggcggg tagtaattga
     9301 tcctgacttt atgataataa tcagaataaa gttcaggata gagatctggc tgaaatatat
     9361 attctaatac gcttaactta cttgcttctt ttgttttgaa tgaataagga tcgtctaaaa
     9421 cctcgccatc tctgtttcca agtatgtttg tggaaaacca gccgtttagt ccgagtagtc
     9481 tggatttaag ccccggggct agaatggttt tcataagagt ctggccggtt ttaaagtcct
     9541 taccgcagat tggtaccttg ttcttttttg caagttcttc cagtgccgga atatcggcag
     9601 aaaggttagg agcgccattt gcatatgggg ttcctgtttt tattgccgca taagcataaa
     9661 tcatgctcgg tgctatatgg gggttatttt ctttaagccc cttttcaaat ttctttatac
     9721 tcttatgaac atctgatttt tccaggtaga cctcagtgct ggcacaccat accatgacta
     9781 gacggtcgag attgttttct tttttaaagg tttcaatgtc ttttattaat tcatttgcca
     9841 gatccatctt ggtcttggct tttttaacat gtctgccgct taggtttttt acaaattttc
     9901 tttcaaatac ggctttattt ggagaaatgt tctgaagggg ttcttttatt tgtgctaaaa
     9961 gttcctgttc caaaactcca gaattcagag ctgcatcata gcagttttca ggataaggat
    10021 cccagccaca aaaaaccaga tcttctatat cataaagtgg aactaaatct tttatgagcg
    10081 gggattgttt ttctgttctt ttcccaagac gaatagagcc aagctgtgta agagacccga
    10141 ttggttttcc gattccagct ttaatagctt caacaccagc aacaaaagtg gtgctgactg
    10201 ctcctccaat accgggcagt aaaacgccca gctttccttt tggagccttg attctctttt
    10261 tacttttttg tttagaagta gcgatcttgt tattacctct ttgagaactg ttgtccgcgt
    10321 ctggcttttc ttttaccata cttcttactt tctaccatac gaggatcacg tgttagaaga
    10381 tcagctgctt tgagtggttt cttgttgttt gcttcatcgg ctttaacaag cgctcttgaa
    10441 agaccatgag cacaggcatc tgcctgtcct gttagtccgc cgccttttac gtgcatcatt
    10501 acattgtatt tatttgttga gccggttagt tcgagaggtt ttaaaacttt taactgccat
    10561 gtttttctag gaaaatgagt gttaacttca cggttgttaa ctgtaattgt accgtcaccc
    10621 ggtcttaag
//
LOCUS       DBNGSV010000016         5531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1110654, whole genome shotgun sequence.
ACCESSION   DBNGSV010000016 DBNGSV010000000
VERSION     DBNGSV010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1110654"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC159U_00800"
     CDS             complement(<1..586)
                     /locus_tag="AC159U_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3335627.1"
                     /translation="MKRIYIIIFLATFLLTATNNLYAEVYYQWTDEKGVVHATDNPDK
                     VPPRYKNSLKVVKEKDTGSKATLNNFIKLVKRNQQTIIFATVSLLGLFILYKLFKGLK
                     NRSSATSKNKYDEVIKKSGIDTMNIPQFKNYVKNVLSAKGYHLTEFEGDLDFGVDFIA
                     DKGNKNYLVKVVSDNMTTSKMVVNDILRDTSKYGC"
     gene            672..1724
                     /locus_tag="AC159U_00805"
     CDS             672..1724
                     /locus_tag="AC159U_00805"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR3335628.1"
                     /translation="MSLYALIMAGGEGKRFWPLSSKDNPKQFLNLTGDNSLLRNTYER
                     ICGLVKNENIFVVTTKNYVNKTAKHLPELNKNNILAEPVGKNTGPCIYYGSNIISDLD
                     KDAVIVVLPADHAISNKNKFLSTLKYAIKICSKPLFKDNYPLVTLGIKPTRPDTGYGY
                     IKKNDRIHGTTRHSTYTVSQFTEKPDGPKAERYIKTGKYFWNSGIFVWKASSILKEFS
                     IHCPQWDSYSHYDFCNKSLLGDYYKKIIAGPVDKMILEKSKNTVVIPASFEWSDIGTW
                     QSLDEYLRKNSNENIQRADSYSLGCSSNMIIGSKKPVVTIGVNDLIVVDSKQGILVIN
                     KNESQKVKHIAEKLGK"
     gene            1783..2340
                     /gene="gmk"
                     /locus_tag="AC159U_00810"
     CDS             1783..2340
                     /gene="gmk"
                     /locus_tag="AC159U_00810"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3335629.1"
                     /translation="MLYVISGPSGSGKTTLTKKLVEEFDSLKFATSYTTRSPREGERD
                     GVDYVFVNEQVFSAMIEENRFIEWAFVHGNYYGTPVDIIRQSHGRNFDVLLDINVDGA
                     NKIKKTGEDAVYIFLKPPSMEILRQRLIDRGDISEPEIDKRLEVVRKEIEKSINYDYI
                     IVNSELEYSFNKLVDIIKARRSIKK"
     gene            2366..4546
                     /locus_tag="AC159U_00815"
     CDS             2366..4546
                     /locus_tag="AC159U_00815"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3335630.1"
                     /translation="MIRSNDIIDKVGAYTTLSDQDVESIQASYVYSAKVHAGQKRVSG
                     EPYLSHPLEVSAILADMKLDVASVITGMLHDTIEDTLATKEEIESLFGKEVAFLVDGV
                     TKISRLSFNTKVEEQAESFRKLILATAKDIRVILVKLADRLHNMRTLEHLNPEKQKRI
                     SKETFDIYAPLAHRLGIYWLRTELEDLSFKYLKSEEYKNINGIIQKKKKEWDKYIDEV
                     KSLLLSKLEESNIEAEISGRMKHSYGIYTKMVNQNIEFDKVYDVIAFRIITDSVSKCY
                     EILGLVHSVWKPVPGRFKDFIALPKLNGYQSLHTTVIGPFGERMEIQIRTKEMHELSE
                     FGISAHWKYKEGKDSKNEFDRVYANLRQLLEWKDIKDPNEFLDAVKGDLIPHMVYIFT
                     PTGDLKEMPNGATPVDFAYSIHTEVGHHCSGSIVNGKIVPLDHTLKSGDTVEIMTRKD
                     KYPNQDWLKFVVTSSAKTKIRSWLRSKEQDQSKMVGKSICERKFKQQGIEFQKLLKGD
                     NFKKVLSDYNFKNVDDLYIAVGFGKISASDVMKYFITNDKDLDKVDKTSRIDKIIKTI
                     TKDSSDGVLVRGYNDILIRISHCCSPLPGEDIVGYITRGRGISIHKSDCPQLFEIDPS
                     RKIDVEWDKSYKGLMSARILVKCNDEPGILSNITKSISSSDINISKAEMSSPDEANAV
                     GTFDINVKDIKQLDKLMMRITKLKGVKSVERIISEDSSGSVAIS"
     gene            4640..>5531
                     /gene="lepB"
                     /locus_tag="AC159U_00820"
     CDS             4640..>5531
                     /gene="lepB"
                     /locus_tag="AC159U_00820"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3335631.1"
                     /translation="MFNIFSEEKKIESSAKQTISDIKSVLVKSDGLIDSSIKNKFGEK
                     ITAIEHSLNQKNIQDTKRLNQELRELESEHLSKFIKSKLRQNIESLIIALFLALFVRE
                     FIVQPFKIPSGSMIPNLLVGDHLLVTKFIYGTKIPFTNIQILPGIKNIEHGDVIVFIY
                     PNGDKEPSKKGVHYIKRVVGLPGDSITIKKRNLYINGKQIPLKFTSSYDSNSLNDTTY
                     VDEYEEDLFNHKHKVIYRNGKKSTDKGIFIPLDKVPEGHYFVMGDNRDNSKDSRYWGL
                     VPFENISGKALLTHWSWDDRN"
BASE COUNT         1988 a          831 c         1065 g         1647 t
ORIGIN      
        1 tacatccata cttggatgta tctcttagaa tatcatttac taccattttt gatgttgtca
       61 tattatctga cactaccttc acaagatagt tcttgtttcc cttatctgct ataaaatcaa
      121 ctccaaagtc aaggtcacct tcgaattctg ttaaatgata acccttggcc gatagcacgt
      181 tcttaacata atttttaaac tgagggatgt tcatcgtatc aattcctgat ttttttataa
      241 cttcgtcgta cttattttta cttgtcgccg aagatctgtt ttttaatccc ttaaataatt
      301 tatagaggat aaaaagtcca agaagtgaga ctgttgcaaa aataattgtt tgctggtttc
      361 gttttactaa ttttataaag ttatttagcg tggcttttga acctgtgtct ttttctttta
      421 ccacttttag tgagttttta tatcttggcg gtactttatc ggggttgtct gtagcatgta
      481 cgactccttt ttcatcagtc cactgataat aaacttctgc atacagatta tttgttgcag
      541 ttagaagaaa tgtagccaag aaaatgataa tatatattct tttcataatc ttttattcga
      601 ttagtactaa tataaaatag acgtattaag agattatata ttaaaataat atatttataa
      661 gggaatagtt tatgtcactt tatgctttaa taatggcagg aggggagggt aaaaggttct
      721 ggccgcttag cagcaaagat aatccaaagc agtttttaaa tctaacaggt gataattctc
      781 tattaaggaa tacatatgaa agaatatgtg gtcttgtaaa aaatgaaaac atatttgtgg
      841 ttactactaa aaactatgtt aacaaaactg caaaacatct tcccgagcta aataaaaata
      901 atatactggc cgagcctgta ggtaaaaata cagggccatg catttattac ggttcaaata
      961 ttatttctga tttagacaaa gacgcagtaa tagtagtgct tccggcagat catgcgattt
     1021 cgaataaaaa caaattttta agtactttga aatacgccat aaaaatatgt tcaaaaccat
     1081 tatttaaaga taactatccg cttgttacac ttggtattaa accaacaagg cctgacaccg
     1141 ggtatggata tataaaaaaa aatgacagga tacatggtac tacccgacat agtacttaca
     1201 cagtctcgca gtttacagaa aaacctgatg gccctaaagc tgagagatat ataaaaacag
     1261 gaaaatattt ttggaatagt gggatatttg tatggaaggc gtcatctata ttaaaagaat
     1321 tcagtattca ctgtccacag tgggatagtt acagtcatta tgacttttgt aataaatctt
     1381 tacttgggga ttattataaa aaaatcatag ccggtccagt ggataaaatg atattggaaa
     1441 aatctaaaaa cacggttgtt attcctgcca gttttgagtg gagtgatata ggtacctggc
     1501 agtcacttga tgaatatttg agaaaaaatt ccaatgagaa catacaaaga gctgattcat
     1561 acagtctggg ttgtagttca aacatgataa taggaagcaa aaaacctgtt gtgaccatag
     1621 gagtaaatga tttgattgta gtagactcta agcagggcat tttggtgatt aataaaaatg
     1681 aatcacagaa agtaaaacat attgcagaaa aactgggcaa atagattaga atatcagatg
     1741 ctaatcaaca gataatcaaa aaacaggtaa taaggcaact atatgctgta tgtaatttca
     1801 ggaccatcag gaagcggtaa aaccacactt actaagaaac tggttgaaga gtttgatagt
     1861 ctcaaattcg caacatccta tacaaccaga agccccagag aaggtgagag agatggagta
     1921 gattatgtgt ttgtaaatga gcaagtattt tctgcgatga tagaagagaa caggtttata
     1981 gaatgggcat ttgtacatgg gaattattac ggaacaccag ttgatataat cagacagtca
     2041 catggcagaa atttcgatgt actgctggat ataaatgtag acggcgctaa taagattaaa
     2101 aaaacaggtg aggatgcggt atatatattt ttaaagcctc catcgatgga gattttaaga
     2161 caaagactca tagatagggg agacatttct gagcctgaaa tagataaaag attggaagta
     2221 gtaagaaaag agatagaaaa aagcatcaac tatgattata ttattgttaa tagtgagtta
     2281 gaatactctt ttaataaatt agttgatata ataaaagctc gaagaagcat aaaaaagtaa
     2341 ataaaattta cttttaaaac aatagatgat tagatcgaat gacataatag ataaggttgg
     2401 tgcttatacc acactctctg atcaggacgt tgagtcgata caggcttctt atgtctattc
     2461 agccaaggtg catgctgggc aaaagagggt atccggagag ccatatttaa gccacccact
     2521 tgaggtatct gcaattcttg ccgatatgaa gcttgatgtt gcttcagtta ttacaggcat
     2581 gctgcatgac acgattgaag acacacttgc gacaaaagaa gagattgaaa gtctttttgg
     2641 aaaagaggta gcttttcttg tagacggtgt taccaaaata agccgtctga gttttaacac
     2701 caaggttgaa gaacaggctg aaagcttcag aaaacttata cttgcaactg caaaggacat
     2761 cagggttatt ctcgtaaaac ttgccgaccg tctccataac atgagaacat tggaacactt
     2821 aaatccagaa aaacaaaaga gaatatcgaa agaaacattc gatatttatg ccccacttgc
     2881 acacagatta gggatttact ggcttagaac tgagcttgag gacctttctt ttaagtattt
     2941 aaaaagcgaa gaatacaaaa acatcaacgg aataatacaa aaaaagaaaa aagaatggga
     3001 caagtatatt gatgaggtca aatctttgct tttatcaaaa ctggaagaat cgaacatcga
     3061 agccgagata tccggaagaa tgaaacattc ttatggaatt tacacaaaaa tggtaaatca
     3121 gaatatcgaa tttgataagg tttacgatgt tattgctttt agaataatta cagattcagt
     3181 cagtaagtgt tatgaaattc tgggtttggt acattcggtc tggaagccgg tacccggtag
     3241 atttaaggat tttattgctc ttcccaaatt aaacggatac caatcacttc atactactgt
     3301 gataggtcct tttggggaga ggatggagat tcagatcaga acaaaggaga tgcacgagct
     3361 ttctgaattt ggaatttctg cacactggaa atataaagaa ggtaaggatt caaaaaatga
     3421 gtttgacagg gtgtatgcaa atttaagaca gctacttgaa tggaaagata tcaaggatcc
     3481 aaatgaattt ctggacgcgg taaaaggaga tttaataccg catatggtct atatatttac
     3541 tccaactggt gatttaaaag agatgccaaa tggtgctaca ccagttgatt ttgcatattc
     3601 tattcataca gaggtgggac accactgttc cggatcaatc gtaaacggta agatagttcc
     3661 gttggatcac accctgaaaa gtggtgatac ggttgagatc atgacaagaa aggataaata
     3721 tcccaaccag gactggctta aattcgtagt tacttcgagc gctaaaacaa agataagatc
     3781 atggcttaga tccaaagaac aggatcagtc gaaaatggtt ggtaaatcaa tttgtgagcg
     3841 taaatttaag caacagggta ttgaatttca gaaattgctc aaaggggata attttaaaaa
     3901 agtgctctca gactacaatt ttaaaaacgt ggatgatcta tacatagctg ttgggtttgg
     3961 gaaaatatct gcttcggatg taatgaaata ttttatcact aatgataaag atttagataa
     4021 ggtagataaa acaagcagaa ttgataaaat aataaaaact attaccaaag atagctcgga
     4081 tggagttttg gttagaggtt ataatgatat cctgataagg atatcacatt gttgttcccc
     4141 gctacctgga gaggatattg ttggttatat aacaagaggc agaggaatat caatacacaa
     4201 gagcgattgt cctcaacttt ttgaaataga tccatcaaga aagatagatg tagaatggga
     4261 caaatcctat aagggcctca tgtcagctcg aattttggta aaatgcaatg atgagcccgg
     4321 aattctttct aatattacaa aatcaatatc gtcatcagac attaatattt ctaaagccga
     4381 aatgtcatcc ccagacgagg caaatgcagt aggaaccttt gacataaatg ttaaggacat
     4441 aaagcagcta gataaactta tgatgcgtat cactaaacta aagggagtaa agtctgtaga
     4501 acgtatcatc tcagaagact cttcaggtag tgttgctatt agttaatagt attaatataa
     4561 gaaatatgaa caaattgcat tattatttta cctaaccgtt gaaactaata tttctgcgtt
     4621 tatattattc gattaaatta tgtttaatat attttctgaa gaaaagaaga tcgagtcatc
     4681 cgctaaacag acaatatctg atataaagtc agtgcttgta aagagtgatg ggctcattga
     4741 ttcaagtata aagaataaat tcggtgagaa aatcaccgct attgagcatt cactgaacca
     4801 aaaaaatata caggatacca aaaggctcaa tcaggaactg cgggagcttg aatctgaaca
     4861 tttatccaaa tttataaaat ccaaactaag acagaatatt gaatcattaa taatagctct
     4921 ttttctggct ttgtttgtaa gagaatttat tgtacagcct tttaaaattc cttccggatc
     4981 gatgattcca aacctgcttg tgggtgatca ccttcttgtc acaaaattta tttatggaac
     5041 caaaatacct tttaccaata tacagatact tccggggata aaaaacatag aacatggtga
     5101 tgttattgtt tttatatatc ctaatggaga taaggaacct tcaaaaaaag gagttcacta
     5161 tattaaaaga gttgttggtc ttcctggaga ttctattact atcaagaaga gaaatttata
     5221 tataaatgga aaacaaatac ccttaaaatt tacaagttct tatgatagta acagcctcaa
     5281 cgacactact tatgtagatg aatatgaaga ggatctgttt aaccacaagc acaaagttat
     5341 atatcgcaat ggtaagaaat ctaccgataa gggtatattt atacctcttg ataaagtacc
     5401 tgaaggacat tactttgtga tgggtgataa cagggataat agtaaagaca gccgatattg
     5461 gggtcttgtt ccatttgaaa atatttcagg caaggcttta ctgacacact ggtcatggga
     5521 tgatagaaat a
//
LOCUS       DBNGSV010000017         2457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_279609, whole genome shotgun sequence.
ACCESSION   DBNGSV010000017 DBNGSV010000000
VERSION     DBNGSV010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_279609"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..272
                     /locus_tag="AC159U_00825"
     CDS             21..272
                     /locus_tag="AC159U_00825"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR3335632.1"
                     /translation="MLFVITQTHAPESCPKDEGGSKTLYNADAEGIELKAMYGAFAEH
                     VIYYVVEADSQDALHKFLLPGFKRCDCTVTPVAEEAIVK"
     gene            complement(279..1634)
                     /locus_tag="AC159U_00830"
     CDS             complement(279..1634)
                     /locus_tag="AC159U_00830"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR3335633.1"
                     /translation="MKGIIIGNKLDKSSLTKYFEDGCKPSSVWKLGSEYESLGVYPES
                     AKACSYSGQRGIEQALIHLSNNTKYSPELNDGHIIALHDKESSITLEPGSQVELSGKQ
                     HSSVHSIKHEIDDHFKELLEVGDKLNIKFINNALQPLSSLDEIELIPKPRYDIMYPYM
                     NEVGTLGHRMMKQSSAIQISIDYSDEADAKKKFKTAMGLVPVFYSIFANSPILEGKLN
                     GYKSYRGHVWTDTDNDRCGILSFPFEDKPIFESYTDYALDAGMYFVLRDGQLKNMTGI
                     TFRHFLDKGHDGHIATPEDWELHISTLFPEVRMKKYIEIRCFDSQPYENMLAVPVMIK
                     GIFYDTVSLDAAWKLVANWSWEERVKAYNSAHKYALQSISRHKSLRELAEELINIAKT
                     GLIKQDIKNELGENESIYLSGIEQDVNNGICPADKIIEKWQNEWGKDFTKLIEHCAYK
                     I"
     gene            complement(1677..2264)
                     /locus_tag="AC159U_00835"
     CDS             complement(1677..2264)
                     /locus_tag="AC159U_00835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3335634.1"
                     /translation="MNRQPVFEYHAETVQVPLSLPGGNTKSITIESGYAFGTGSHFTT
                     RLCLKMIEQIYEERILKNVLDVGCGSAILAISTIALGAKNALAVDIEPTVIGEARKNV
                     DKNGYSAQITVQNTKINDIEDRFDLVLANILTDQILKIADDLVSRVNVKGYLILSGIR
                     INEKEVIIDKLTKLNTEFKKILTDDDWCSLLFSKF"
BASE COUNT          753 a          462 c          416 g          826 t
ORIGIN      
        1 atatttaaag gagaaaacca atgttatttg taatcacaca gactcatgcg ccggaatcat
       61 gtccaaagga tgaaggggga tcgaagacgc tttataatgc agatgcggag ggtatagaat
      121 taaaagcaat gtatggagcg tttgctgaac atgtgatcta ctatgtagtt gaggctgata
      181 gtcaggatgc tcttcacaaa tttcttctac ctggttttaa acgctgcgac tgtactgtta
      241 ctcccgtggc agaagaagca attgtgaaat gagtgttatt aaatcttata ggcacagtgc
      301 tctattaatt ttgtaaaatc tttgccccat tcattttgcc atttttctat aattttatcg
      361 gcagggcaga tgccattgtt aacatcttgt tctatgccgc ttagataaat agattcgttt
      421 tcaccaagct cattctttat atcttgtttt attaggccgg ttttggcaat attaataagt
      481 tcttcggcaa gctcacgaag agatttgtgc ctgcttatag attgaagtgc atacttatga
      541 gctgaattat aggctttaac ccgttcctcc caactccaat tagcaactaa tttccatgct
      601 gcatcaagtg acacagtatc ataaaaaatc cccttgatca taacaggaac tgcaagcatg
      661 ttctcgtatg gctgactgtc aaaacatctg atttctatat attttttcat cctaacctct
      721 ggaaaaagcg tagaaatgtg tagttcccag tcctcaggtg tagctatgtg gccatcatga
      781 cctttatcta aaaaatgacg gaaggtgata cccgtcatgt tctttagctg tccgtcacgc
      841 agcacaaagt acatccctgc atcaagtgca taatcagtat aagattcaaa tatcggttta
      901 tcttcaaaag gaaatgacaa aataccacac ctgtcattat cagtatctgt ccatacatgc
      961 cctctatagg acttatagcc attgagcttt ccctctaata taggagagtt ggcaaatatg
     1021 gaataaaaaa caggaactaa tcccattgca gttttaaatt ttttctttgc gtctgcttcg
     1081 tccgaatagt ctatgcttat ctgaatagcc gatgactgct tcatcattct gtgcccaaga
     1141 gtgcctactt cattcatata aggatacata atgtcatatc ttggttttgg aataagctca
     1201 atttcatcaa gagagctcag aggctgtaga gcattgttta taaactttat gttaagctta
     1261 tctcccactt ccaataactc tttgaaatga tcatctattt catgttttat ggaatgtaca
     1321 gagctatgct gttttcctga tagttcaacc tgacttccgg gttcaagtgt aatgctggat
     1381 tctttatcgt gtaaggcaat gatgtgacca tcatttagtt caggactgta cttagtgttg
     1441 ttgctaagat gaataagagc ttgttctata cccctttgcc cggaataact gcaagccttt
     1501 gccgattcag ggtaaacacc aagtgactca tattctgagc caagtttcca gaccgaagat
     1561 ggtttgcaac catcttcaaa atatttagtt agtgaagact tatccagctt attgcctatg
     1621 atgattccct ttatatttta attaagaatg aaacacaaga ttatttacat ataaaattag
     1681 aatttactga acaataatga acaccaatca tcatcagtca agattttttt aaactccgta
     1741 ttaagtttag taagtttatc aataatcact tctttttcat taattctaat tcctgataat
     1801 attaaatatc ctttaacgtt tactctggat acaagatcat cagcaatttt taaaatttga
     1861 tctgtaagta tattagctaa taccagatcg aatctgtctt cgatatcatt tattttagta
     1921 ttttgaacag tgatttgggc ggaataacca ttcttgtcaa cattttttct tgcctctcca
     1981 attacagttg gctcaatatc aacagctaaa gcattcttgg caccaagggc aatagtcgat
     2041 atagccagta tcgcgcttcc gcacccaaca tctaggacat ttttcaatat tctttcttca
     2101 tatatctgct ctatcatttt tagacaaagc ctagtagtaa aatgactacc tgtaccaaat
     2161 gcatatcctg actcaatggt tatgctttta gtattcccac ccggtagcga taaaggaact
     2221 tgtacagttt ctgcatgata ttcaaataca ggttgtctat tcatatttct ttgatataga
     2281 ttgtcataaa gaattaaaat atatttactg ttttattaat aattgctagt ataattttag
     2341 gagctcgcat aggacagcaa agtatactaa gctgaaacta tgcaaaagaa gttacttaag
     2401 aatacaaaac tatcaaatag taagataagc aagctaatac aaatgtttgt attggaa
//
LOCUS       DBNGSV010000018         8922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_556503, whole genome shotgun sequence.
ACCESSION   DBNGSV010000018 DBNGSV010000000
VERSION     DBNGSV010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_556503"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC159U_00840"
     CDS             <1..421
                     /locus_tag="AC159U_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335635.1"
                     /translation="NYFALSILFVIILKTNREDVMKKIAIYTLLLNLIATGLILVNTA
                     SADTGLKQVESKYVCMITNKLFADEQILVDIEGRKYYGCCEMCKGKLEQDIASRKSFD
                     PVSGKEVDKATAIIGAAANSSVYYFENEDNLKAFNSK"
     gene            complement(428..1342)
                     /gene="trhO"
                     /locus_tag="AC159U_00845"
                     /gene_synonym="yceA"
     CDS             complement(428..1342)
                     /gene="trhO"
                     /locus_tag="AC159U_00845"
                     /gene_synonym="yceA"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF001133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent tRNA uridine(34) hydroxylase
                     TrhO"
                     /protein_id="HIR3335636.1"
                     /translation="MSNIHVILFYKFTPIEDPKRFSKHHLRFCKELGVLGKILVATEG
                     INGSISGSKDQIESYKSNLRSQSQFADINFKEEISTFHPFKKMIVRVKKEIIRMDKEL
                     DLEKTGKYITPKELIELYNSDEEFVVLDTRNNYESDVGKFKGAITPDIDSFRDFPKVI
                     SDLEDKKDKKIITYCTGGIRCEKATAYMVQEGFSDVYQLKDGILNFCKEYPNTLWEGK
                     CFVFDQRLLSHVDPDSEIISQCSVCGDKSDRYINCKNVECDDLVVLCEKCGKELSECC
                     SKECAVTHKNFLKDKSYKNQGYKSKVSA"
     gene            1466..3379
                     /locus_tag="AC159U_00850"
     CDS             1466..3379
                     /locus_tag="AC159U_00850"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3335637.1"
                     /translation="MSDLINKVIRHYKITDEIALGGMGKVYKATNQKLNRTVAIKVVN
                     PNLISSPSIINRFYNEAKIQAKLSHINIVTVYDFFEHNNNHFLVMEYMGGESVDKIIK
                     KNGPFDIVTSISMMKQMLAGISHAHSKGVIHKDIKTSNFILTPTVIKITDFGISQLIT
                     ESSNETTRMGTLDYMSPEQLLDNKIDYRTDLYSLSITFYELLTGRSPYNIEYSSENEI
                     KNAHLTVDPVPITSIIKNFPPQLNQMILKGLSKDPNTRFQSAADYLIAIDKFVENNKS
                     RKINTIEISEGKHSTGINFKNVNFSFNGEIQDVPFHKLLVDIFNSKKTGFLFIDSEIK
                     LQIYFQNGYIANVSGIIDDLTLGELLVRSSIITEEDQKQAINFSLETGLKIGEVLITM
                     NKISSHDLSSILEVQIKDKLNRGFLLRKGKYAFKDLDEIRLNIAYNIHPLQVIYNHVI
                     AAKYKGLDEDLLNNKLNSDYIVCSDIEDKLRGVVFSQSKEVRIIDQLKNINKEINPVK
                     DINIDKETKFNLLHFLTISGLIESAHLSESDNTDLNTNISKQKPNETKKVKYEIKRTL
                     SNGKTLSYKEELDVPTKTQDDDTTTKDNKGILGLDKEYKDINKIPKEDDDDDKTVIFT
                     PEQREEIVRKNNK"
     gene            3546..5369
                     /gene="typA"
                     /locus_tag="AC159U_00855"
     CDS             3546..5369
                     /gene="typA"
                     /locus_tag="AC159U_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3335638.1"
                     /translation="MIKNNLRNIAIIAHVDHGKTTLVDVMLGQSGVFGEHQHVEERVM
                     DSMDLEKERGITIMAKNTALMYKDIKINIVDTPGHADFGGEVERSLNMVDGALLLVDS
                     SEGPLPQTRFVVKKALEKKLPIILVINKIDRADARPEEVIDEVYDLFIDLDADEQQIE
                     FPIVYTNAKLGIALDKLNEETKDLKMLFETIVNSIPGPDCDNSADTQFLITNLDYDNY
                     VGRIAIGRLMNGVLESNKTYTLCTDNDQTTNIKTSVCYTYQGLNKIKSEKIEAGDIAA
                     IAGVDEIDIGDTICTLDKPAPLPRIKVDEPTISMIFYVNNGPFAGKEGKFLTSRHIRE
                     RLEREQIRNVAIKVRDTDRADAFEVAGRGELQMAVLIETMRREGYELMVSKPQVITKE
                     ENGKITEPVERIFVDVPEDNVGVVTEKVAERKGRMVNLRNNGNGRVDIEFIITSRGLI
                     GFRSQFQIDTKGAGVLNTLFEGYEPWYGPIPQRSTGALVSDRSGKSNTFASLAMVDRG
                     ELFIDVGTPVYEGMIIGERNRSGDLNVNITKEKKLTNMRAASSEATVVLRPPRPLSLD
                     QAIEFIAEDELVEVTPLCHRLRKMELNAAQRAINTKRAKKE"
     gene            complement(5378..6373)
                     /locus_tag="AC159U_00860"
     CDS             complement(5378..6373)
                     /locus_tag="AC159U_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HIR3335639.1"
                     /translation="MATNFKALVVSKNEDDKYIREIKERSTDDLPEGEVLVKVNYSSL
                     NYKDALSAIGSKGVTRNYPHTPGVDSSGVVEQSSSDGIKAGDEVIVHAYDLGVNTSGG
                     FGQYIRVPSDWVVKLPNNLSQREAMIFGTAGFTAAQCIYKMEKNGVTPSDGEILVTGA
                     TGGVGSTAVAILSKIGYDVVASTGKTDQAEFLKDIGAKEVISREDSVDLSRPLLKGRW
                     AGVVDTVGGDILATAIHSTKQHGTVTCCGNVGGHQLNTNVYPFILRGVDLLGVDSQNY
                     PMEGRVTIWNKMANTWKTSNLEKLCNEISLEELNENIDLILEGKQKGRVVVNLWK"
     gene            complement(6402..7049)
                     /locus_tag="AC159U_00865"
     CDS             complement(6402..7049)
                     /locus_tag="AC159U_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335640.1"
                     /translation="MKILLTFIIAAFVTASVLPSFAAPGDKSKGQQKKEQKMKEHKSN
                     KGKQKGNDMDSEDLEDIHEEMGDSKKINKESKGKSKNKGKRKKVEVNDPENSDESDDV
                     DESQESENKSNKGKDKDNKEKGNNKSNDNNDEEDEDVDDPDIDEQEEDNDDKDGSDEE
                     EKGNKIKKEKDEKTNKGLDKQREKKSEQEMKELGKGSEQGQESRQKRKKWWKFWE"
     gene            complement(7127..8710)
                     /locus_tag="AC159U_00870"
     CDS             complement(7127..8710)
                     /locus_tag="AC159U_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014719.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR3335641.1"
                     /translation="MIEQIGLFLAGLGLFIAGLRILSSSLQEISSGRIRHLFKWINNR
                     VIGVFWGLVAGAISQSAAGITVIVSIMVSNNVLNLTTGLSMVGWLGVGTTLLVFLVSF
                     NIKFAVLYLMGITGILYGFQKSSRRKLYMSALLGVAMLLYGFQIIGSSGKNFSDLTVF
                     QYLFEYQNLGLIIAFVIGAVLRVVLQSQAGVVLLAITIGLAGEISFYQIMMIVYGSYL
                     ASGPIMYMFSKGVSGRAREICIYKSLTYIFTSVVMVFVLVFERYFDLAGVKYVIEMFI
                     VSLELKVAMVYMLSCIVEGLISEVFMSPIYKLAQRLTPVDHTEGLSTSKYITSFIDDV
                     EASLDLVDLEKQRIIRRFPKIIDNIRKETSESEIVDNEILYNANRALIKNIKQYLSET
                     YKSKMNKTTADRLMKLQTNLDLIYGLNNVLNEFVTALKSACNVDEIIESEDVLIESLH
                     LLCLTMNDAARSQSQEDLILLSTLTSDKGDVMHKIRENFIKYNEANEKVDKQRFLHLT
                     NLYQRSVWLFNRWAHLNQS"
BASE COUNT         2949 a         1658 c         1477 g         2838 t
ORIGIN      
        1 aaactatttt gctctatcaa tattatttgt tattatttta aaaactaata gagaggatgt
       61 tatgaaaaaa atagccattt atacactact tctaaacctg atagcaacag gtctaatttt
      121 agtaaacact gcatctgccg atacgggatt aaaacaggtc gaatcaaaat atgtatgtat
      181 gattacaaac aagctttttg cagacgagca gatcttggta gacattgaag gcaggaaata
      241 ctatggatgc tgtgagatgt gcaaaggcaa gcttgaacaa gacattgcca gcagaaaatc
      301 atttgatccg gtttctggca aagaagtaga caaagccaca gcgattatag gagctgcagc
      361 taacagcagt gtatattatt ttgagaatga agataatctg aaggctttta attcaaaata
      421 attattttta agcagaaact tttgatttat aaccctgatt tttgtaggat ttatctttta
      481 gaaagttttt atgtgtaact gcgcactctt ttgagcagca ttcactaagt tctttaccgc
      541 atttttcaca tagtactaca agatcatcac attctacatt cttgcagttt atataacggt
      601 ctgatttatc tccgcacact gagcactgag atattatctc actgtcaggg tcaacgtgtg
      661 atagaagcct ctgatcaaac acaaagcact tcccttccca tagagtattg ggatactctt
      721 tacaaaagtt aagtattccg tcctttaact gatacacatc agaaaaaccc tcttgaacca
      781 tataagctgt agccttttcg caccttatgc cacctgtgca gtaggtaatt atcttcttat
      841 cttttttgtc ttctaaatct gaaatcactt ttggaaaatc cctgaatgaa tcaatatccg
      901 gagtaatagc gcccttaaat ttaccaacat ccgattcgta gttatttctg gtatcaagca
      961 caacgaactc ttcatcgctg ttatataatt ctatcaattc ttttggtgtg atatatttgc
     1021 ctgtcttttc taaatccagt tccttatcca ttctgattat ctctttttta acacggacaa
     1081 tcattttctt aaagggatga aatgtactta tttcttcttt aaagttaatg tctgcaaatt
     1141 gggattgtga cctaagatta ctcttatagg attctatttg gtcttttgag cccgatatcg
     1201 atccatttat gccttcagtt gctactaaga ttttgcccaa aactccaagc tctttacaaa
     1261 acctcaggtg atgcttggaa aacctcttcg gatcttcaat cggtgtaaat ttataaaaca
     1321 gaattacatg aatattactc ataagttaca aataaattac tcaaaatttc gctaatgtta
     1381 atagtctaaa ttaaatataa ataaatttgt gagttactat atttatttat tctcgttata
     1441 ctactaggat ttataattta tatagatgag cgatttgata aataaagtaa ttcgtcatta
     1501 taaaattact gacgagattg cccttggggg aatgggcaag gtctataaag caactaacca
     1561 aaagcttaac agaacagttg cgataaaagt agtaaatccc aaccttataa gtagcccaag
     1621 cattatcaac agattttata atgaagcaaa aatacaggca aaactctctc atatcaatat
     1681 agttacagtg tatgattttt ttgagcataa caataaccat tttcttgtca tggaatacat
     1741 gggcggagag agtgttgata aaataatcaa aaagaacggg ccttttgata tagtaacttc
     1801 tatttcaatg atgaagcaga tgcttgcagg cataagccat gcgcactcaa aaggtgttat
     1861 tcataaagac ataaagacca gtaattttat acttacccct acagtaataa aaattacaga
     1921 ttttggcatt tcacagttaa taaccgaaag cagtaatgaa acaacaagaa tgggtactct
     1981 ggactacatg tctcctgagc agcttttgga caataagatt gattacagga cagacttata
     2041 ttcattatcc attacatttt atgaactctt aacaggcagg tctccttata atattgagta
     2101 tagctcggaa aatgaaatca aaaatgctca tttaactgta gaccctgtac ctattacatc
     2161 aattataaag aattttcctc ctcagctcaa tcagatgatt ctaaaaggtc tatcgaagga
     2221 tccaaatacc aggttccagt cggctgctga ctatctcata gccatagata agtttgttga
     2281 gaataataag tccagaaaaa taaacactat tgaaatttca gagggaaagc attctaccgg
     2341 aattaatttt aaaaatgtta atttctcatt taatggtgag atacaggatg ttccatttca
     2401 taaattactg gttgatatat ttaatagtaa gaaaactggt tttttattta ttgattcgga
     2461 gataaaactt cagatttatt ttcaaaacgg ctatatagcc aatgtaagtg ggattattga
     2521 tgatcttact cttggtgagc tgcttgtaag aagctcaata atcactgaag aagatcagaa
     2581 acaggccata aacttttcac tagaaactgg tcttaagata ggagaagttc ttatcacaat
     2641 gaacaaaatt tcttctcatg atctaagctc aattctagag gtacaaatta aagataaatt
     2701 aaacagaggg tttttactca gaaaaggtaa atacgcattt aaagatcttg atgaaataag
     2761 gcttaatatt gcatacaata tccaccctct gcaggtaatt tacaatcatg taatcgccgc
     2821 aaaatataag gggctcgatg aagatcttct taataataaa ctaaattcag actacatagt
     2881 ctgcagcgat atagaagata aattgagggg tgttgtattt tcacaatcaa aagaagtaag
     2941 aataatagac cagctaaaga acatcaacaa agagataaat ccggtaaaag atataaatat
     3001 agacaaagaa actaaattta acctccttca ttttctaaca atatcaggac taatagagtc
     3061 agcacattta tctgagagtg ataataccga tttaaacacc aatatatcaa aacaaaagcc
     3121 taacgagacc aaaaaagtaa aatatgaaat aaaacgcaca ttatcaaacg gcaagactct
     3181 atcttataaa gaagagctgg atgtaccaac caagacacaa gatgatgata ctactactaa
     3241 agataataaa ggaatattgg gactagataa agaatataaa gatattaata aaattccaaa
     3301 agaagatgat gatgacgata aaactgtaat atttacccct gagcaaagag aagaaatagt
     3361 cagaaaaaac aataaataat tactcttttc ataaaagtta tattattgct aacttataca
     3421 tattaattag atatcaatac tttttgatat tctctctata gatatatttt ctccctcttg
     3481 taataaaatt aatatagtta ataatgtacg accttttaga accgggagat ttagtataaa
     3541 gagttatgat aaaaaacaat ttaagaaata tagctattat tgcacacgtt gatcacggca
     3601 aaactactct tgtggacgtg atgcttggac aaagcggagt gttcggagag caccagcatg
     3661 tagaagaacg ggtaatggac tcaatggatc tggaaaagga gcgcggtata accattatgg
     3721 caaaaaatac cgcactcatg tataaagata taaagattaa tattgtggat acccctgggc
     3781 atgctgattt tggtggggaa gtagaaagaa gccttaatat ggtagacggc gctctgcttt
     3841 tggtagattc aagtgaaggt ccgctgccac aaacaagatt tgttgtaaaa aaagcattag
     3901 agaaaaaact tccgattatc cttgtaatta acaagataga ccgggccgac gccagacctg
     3961 aagaggtaat agatgaagta tatgatcttt ttattgacct tgatgcagac gaacagcaga
     4021 ttgaatttcc tatagtttat acaaatgcaa aacttggtat tgcactcgat aagctgaatg
     4081 aagaaacaaa agacctcaaa atgctttttg agactatagt aaattccatt ccgggtcctg
     4141 attgtgataa tagcgctgat acacagtttc ttatcaccaa tcttgattat gacaactatg
     4201 taggaaggat tgctattgga aggttaatga atggagtatt ggaatcaaac aagacttata
     4261 ctctttgtac cgataacgat caaactacaa atattaaaac ttctgtgtgc tatacatatc
     4321 agggattaaa taaaataaag tctgaaaaaa tagaggcagg agatatagct gctatagccg
     4381 gtgttgatga aatcgatatt ggagacacaa tctgcacact tgataaaccc gcaccactac
     4441 cgaggataaa agtggatgaa cccacgatat ctatgatctt ttatgtaaat aacggcccat
     4501 ttgccggaaa ggaaggaaaa tttttaactt ccaggcatat aagggagcgg ctcgaacgtg
     4561 aacagataag aaatgtagca ataaaagtaa gagacacaga cagagccgat gcctttgagg
     4621 tcgccgggag gggtgagctt cagatggcag tgctcattga aaccatgagg cgggaaggat
     4681 atgaactcat ggtatccaag ccccaggtaa taacaaagga agaaaacggt aaaatcacag
     4741 aacctgttga aagaattttt gtagatgtac cggaggataa cgtaggtgta gttaccgaaa
     4801 aggttgccga aaggaagggc cgcatggtaa acctcagaaa caatggaaac ggcagggtag
     4861 acatagaatt tattattaca tcaaggggac taattggatt taggagtcag ttccagatag
     4921 acaccaaagg agctggcgtc ttaaacacct tatttgaagg ttatgagcca tggtatggcc
     4981 ctatccctca aagatcaaca ggagcgcttg tgtctgacag atcgggcaaa tccaacacct
     5041 ttgcaagcct tgccatggtt gatagaggtg agctgtttat tgatgtgggt acaccggttt
     5101 atgagggaat gattattggg gagagaaaca gaagcggtga cttaaacgtt aatatcacaa
     5161 aggaaaagaa attaacaaat atgagagcgg cttcttcaga agctacagtt gtgctgagac
     5221 ctccaagacc cctatcactg gatcaggcaa tagaatttat tgctgaagac gagcttgtag
     5281 aagtaacacc tctttgtcac agactgagaa aaatggagct aaatgccgct caaagagcca
     5341 taaataccaa gagagctaaa aaagaatagc aatactttta tttccagaga ttaacaacca
     5401 ccctgccctt ttgttttcct tcaaggataa ggtctatatt ttcgtttaac tcttcaagtg
     5461 atatctcatt acataatttt tccagattac ttgttttcca tgtatttgcc attttattcc
     5521 atatcgtcac tctgccttcc atggggtagt tctgcgaatc aacaccaagc agatcaacgc
     5581 ctcttagaat aaatgggtac acattggtat ttaactgatg cccgcctaca tttccgcagc
     5641 atgtaactgt accatgctgt ttagtggaat gtattgcagt tgcaaggatg tctcctccta
     5701 ctgtatcaac taccccggcc catctgcctt tcaaaagggg tctcgataaa tctaccgaat
     5761 cttctctgga tatgacttcc tttgcaccta tatcttttag aaactcagcc tgatcagttt
     5821 ttccggtgga tgccactaca tcgtatccaa tctttgaaag tattgctact gccgtgcttc
     5881 caactcctcc agttgcacct gttactaata tctctccatc agacggtgta acaccatttt
     5941 tttccatttt atatatacac tgagcagccg taaatccggc agtaccaaaa atcatagctt
     6001 ctctttgaga aaggttattt ggtaatttaa ctacccaatc ggaaggaact cttatgtact
     6061 gtccaaatcc tcccgaggta tttactccca gatcataagc atggactatt acttcatcac
     6121 cagccttaat accatccgaa gatgattgtt ctactactcc tgaagagtcc acgccaggag
     6181 tatgggggta atttcttgta acacccttac tgcctatagc agaaagagcg tctttataat
     6241 taagagatga ataatttact tttacaagaa cctcgccttc tggaagatca tcagtagatc
     6301 tttcctttat ttctctaatg tatttgtcat cttcattttt acttacaacg agcgctttaa
     6361 aattagttgc caatgtatta tccctctcta ttatttatat attattccca gaacttccac
     6421 cattttttcc ttttttgtct tgattcctgc ccctgctcag atcccttacc gagttctttc
     6481 atttcctgct cagatttctt ctctctttgt ttatcaagac ccttgtttgt tttctcatct
     6541 ttttcttttt ttatcttatt tcccttttct tcttcatcac taccgtcttt atcatcatta
     6601 tcttcttcct gttcatctat atcaggatca tccacatcct catcttcttc atcattatta
     6661 tcgttcgatt tattattacc tttttcttta ttatccttat ccttaccctt attgctcttg
     6721 ttttctgatt cctgagactc atctacatca tctgattcat cactgttttc tggatcattc
     6781 acttcaactt tttttctctt tcctttattt ttagatttac ctttgctttc cttgttaatc
     6841 ttctttgaat cacccatctc ttcatggata tcttctaaat cttctgagtc catgtcatta
     6901 cccttttgtt tccctttatt gcttttatgc tccttcatct tctgctcttt tttctgctgc
     6961 cctttggatt tgtcgccagg tgctgcaaat gaaggtaaaa ctgaagctgt aacgaatgct
     7021 gctatgataa atgttaatag aattttcata tatacttcct cccgtaaaat tatactaatt
     7081 ataataactt agatattgat ttaaagttta cacataaatt tattttctaa ctttgattta
     7141 aatgcgccca cctgttaaat agccacacag atctttgata taagtttgta agatgtaaaa
     7201 acctctgttt atctactttt tcatttgctt cattatattt aataaaattt tctctaattt
     7261 tatgcatcac atctcctttg tcagaagtaa gagtactgag aagaatcaaa tcctcttggc
     7321 tttgtgacct tgcagcatca ttcatggtta gacaaagcag atgcaggctt tctattagaa
     7381 catcttccga ttctattatt tcatcgacat tgcacgctga ttttagagct gttacaaatt
     7441 catttagaac attgttaaga ccataaatca aatccagatt tgtctgtagt ttcataagcc
     7501 tgtcagccgt ggttttattc attttagatt tataagtttc agataaatac tgtttaatgt
     7561 ttttaattaa agccctgttg gcattatata gtatttcatt atctacaatc tctgattctg
     7621 atgtttcttt tcttatattg tctataattt tagggaatct tctgattatt ctttgttttt
     7681 ccaaatcgac taaatcaagt gaagcttcta catcgtcaat aaaacttgtt atatactttg
     7741 aagtggatag tccttcagta tgatcaaccg gtgttagtct ttgagcaagt ttatagatcg
     7801 gagacataaa tacttctgaa attagtcctt caactataca gcttagcatg tataccatag
     7861 ccacttttaa ttcaagagat actatgaaca tttcaattac atattttaca cccgctaaat
     7921 caaaatacct ttcaaatacg agaacaaata ccattactac tgaagtaaaa atataggtaa
     7981 gtgatttata tatgcatatt tctcttgctc taccgctaac tcctttagaa aacatataca
     8041 ttattggacc tgaagccaga taggacccat atacaatcat catgatctga tagaacgata
     8101 tttcacctgc aagacctatc gtaatggcca aaagcacaac accagcctgt gattgtagga
     8161 caactcttaa tacagcccct attacaaatg caataatcaa acccaaattc tgatattcaa
     8221 aaagatactg aaatacagtt agatcactga agttcttacc gcttgatcca attatctgaa
     8281 aaccataaag tagcattgca acgccaagaa gtgcgctcat gtatagcttt cttctactgc
     8341 tcttttgaaa gccgtaaaga attcctgtta ttcccatcag atagagtact gcaaatttga
     8401 tattaaaaga gaccagaaaa acaagaagag tagttccaac cccaagccag ccaaccatac
     8461 taaggcccgt tgtcaggttt aaaacattat tggataccat aatggaaaca attacagtaa
     8521 ttcctgctgc actctgtgat attgctccgg caaccaaacc ccaaaacacc cctattaccc
     8581 tgttattaat ccatttaaac agatgtctta ttctaccact tgagatttcc tgaaggctgg
     8641 agcttagtat cctaagacct gctataaaaa gacctagacc tgcaagaaat agccctattt
     8701 gttcgatcat atattagtaa ttataatact ccaagactat cctgataatt gtatatacta
     8761 tatatattta aaattagcat atatgataat aattaagggc attagacgtt gttaggagta
     8821 gtatatatga taaagagcaa tgataaagcg gtattcatgg caattaacag tgggaaaaat
     8881 atcacgaatt ttttcacacg caaaatatgg aaaaaacaag ac
//
LOCUS       DBNGSV010000019         6943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_210325, whole genome shotgun sequence.
ACCESSION   DBNGSV010000019 DBNGSV010000000
VERSION     DBNGSV010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_210325"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="AC159U_00875"
     CDS             <1..1223
                     /locus_tag="AC159U_00875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3335642.1"
                     /translation="AQSIYSFRGADFKNVHYFTNRIPNSKVLRLEKNYRSTKGILELS
                     NWLLNESTLDYNKHLESIRGGGITPSIIDFENDFEEARWIAQDLVEKHDKGNRWDDQM
                     VITRSGLASRTLEAILIEKQVPYKFIGGTSLLQSAHVKDLLSLMRCSASHHDEIAWIR
                     YLTLFPKIGDITALRIIEEMKKAPLFNEAIEILRDNKKIMPEILAGPHKVIEKWAVPH
                     DAIRAGIDVLEPLLKTKYSKWDTRRKDFDLLARLAKRHKNLLSFIETFTMDPISTSEA
                     SRLENDDAVTLITVHSAKGTEAKTCYLIKVEPGMYPHVRSLGKLDDEEEERRVLYVAM
                     TRAQDELIITRNLSQGGRYNFHGGYRAEHSRDVSPYFLERIPDNLVDTSYVGFGSYGM
                     QNSDFIKPWDREGY"
     gene            1268..2032
                     /locus_tag="AC159U_00880"
     CDS             1268..2032
                     /locus_tag="AC159U_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335643.1"
                     /translation="MRIILSVLILTLLFVLSGYNSVNARSGSNKSAYSKSGHRKQSAQ
                     QFKSGKHSGGKHYNRQQKSHYNNGKYSGHHKYYKGHKSGHYAKKHHYKHHNKHKGSSY
                     GIYYGGNNLSFGYYGYNRPYYKPYGYYGYGYKPYYSYPTYYYSYDRGYYDRKYSQDRY
                     YDNQIYSEEIEPGRDDSQYEYLEEKPAEQYSEERYSNLKEDTEDYYPEEEYEDDSGEP
                     EYVYKWIDNDGTEHYTNNPKKVPEEFRETVVKLEVW"
     gene            2157..3314
                     /locus_tag="AC159U_00885"
     CDS             2157..3314
                     /locus_tag="AC159U_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3335644.1"
                     /translation="MIFLSLLGLFKLNVLAGLSANYIEVYSIIVLASSFLIGLSFKRS
                     RLIYSIIIISLAGFVLYKEFVTVNLNLFLLSLCLPLNFFVIYNMHERGVLQLSFWKYS
                     ILLVIQILLYYYISSHEKVFADNVFDVSTIDKPFFSDLLLSKFSFAIYISVLIYFLAD
                     FIYKKDFSDSGFFWSFLTTIFMFSEQFNTWNQIFCLSTSGLILLVSFVLRSYDVAYKD
                     KLTELPSRRSFDEAISGLGKEYSIGLVDIDFFKKFNDRYGHDVGDQVLKMVASRLSKV
                     GGGGKAYRYGGEEFAILFANRDKEHSYEHLENLRKDIEKQQFSVRGKKRPKKKPKIPV
                     FFSKDKTEKITVSIGVAQKTEKLSEVQDVIKAADNALYKAKNEGRNRVCLK"
     gene            3342..4496
                     /locus_tag="AC159U_00890"
     CDS             3342..4496
                     /locus_tag="AC159U_00890"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR3335645.1"
                     /translation="MKDYFSSGFKIGILGGGQLGKMLIQAASCWDIDIYVLDPTEGCP
                     ASNIATKFIKGDFKDFDDVYNFGKQVDLVTIEIEHVNTEALLKLKEEGKTICPDPDIL
                     KIIQDKGKQKNFFKNNNLRTANYKLYYNQQTILNDLDYKKLETPFVQKACRFGYDGRG
                     VQIVKNEDDLENLFDGQSVIEDKVYIKKELSVIVARNHSGEIKSYVPFEMEFNEQANL
                     VEDLISPARIDNESAQQAIKLAEQTIKTFGLVGVLAVEMFLDKENNILINEVAPRPHN
                     SGHHTIETALTSQYEQHIRAILDLPLGSTQLISSAVMVNILGEDGFSGQVRYEGLDKC
                     IGIDGVKIHIYGKKQTKPFRKMGHATIIDKDINEAIKKAQRLKLNLRAVS"
     gene            4493..5020
                     /gene="purE"
                     /locus_tag="AC159U_00895"
     CDS             4493..5020
                     /gene="purE"
                     /locus_tag="AC159U_00895"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR3335646.1"
                     /translation="MKKPLVSIIMGSDSDLSVMQEAAQFLDEMGIASELTIVSAHRTP
                     ERMFKFAKTAHTRGIKVIIAGAGGAAHLPGMVASLSPLPVIGVPIKSSNSIDGWDSVL
                     SILQMPAGVPVATVALNGARNAGILAAQIVGTGNKRTQNKISEYKDSLEKGVETKYKR
                     LEKLGFKQYLLKYKK"
     gene            5029..6048
                     /locus_tag="AC159U_00900"
     CDS             5029..6048
                     /locus_tag="AC159U_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3335647.1"
                     /translation="MFTKNSITIISFALILYFTALTNLLHAQVYKCVDSNGNVLYSNS
                     PCPQNTEEQNYKPRESFTINDETTDPEIEDLTETEQTSQNFGAGELDINIVQVRLSLP
                     AQQITAIHKGVHFVYDYFKEVYGITPGTDINIKIFGRHGDFLAYQKRLIGKIYSESGI
                     FSSRTGEALVNASKYRNEVVGISIHEACHAMIQQAGYRIPVWVNEGIAEYFETIEISD
                     NQVLFRPQYDRHSEIASLLSKKELISLLEYFGLSNNVWKNRNLVEDRTSRSIAYSLVH
                     YLMSSVEGTESIKKILKNFSVSDETPSMFVVNQSYSGGLNLLEKNWHEYLTQTPSGHV
                     FSTNQ"
     gene            6167..>6943
                     /locus_tag="AC159U_00905"
     CDS             6167..>6943
                     /locus_tag="AC159U_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3335648.1"
                     /translation="MAESGTNKHEFQAEVKQLLDIVVHSLYTDKEVFVRELVSNASDA
                     LEKLRHIKLTEKDIFDEQLDLEIEITTDDKANIITIKDFGVGMTEDELVENLGTIAHS
                     GSKAFLEAVKKGEEINSNLIGQFGVGFYSAFMVAKKVEVFTHNWKEDAKHLRWESDGS
                     GSYEISEEPGQRRGCKIVIHLKEEDKEFAEPEKIKGILTRYSSFVQFPIKLNGEQVNT
                     VQAIWMRNKNEIKDEEYTEFYKFQGNAFDEPRYTLHFSADA"
BASE COUNT         2386 a         1290 c         1427 g         1840 t
ORIGIN      
        1 atgcacagag catctactca ttcaggggtg cggactttaa gaatgtccat tatttcacaa
       61 acagaattcc aaattccaaa gtacttaggc ttgaaaaaaa ttacagatca acgaagggta
      121 ttctcgaact ctcaaactgg cttctaaatg aatccactct tgattacaat aaacatctcg
      181 aatcaataag gggtggtggg atcacgccat caataataga cttcgagaac gactttgaag
      241 aagcacgctg gattgctcag gatctggtcg aaaaacacga taagggcaac cgctgggacg
      301 atcagatggt catcacaaga agcggtcttg catcaagaac cttagaagca atcttaatcg
      361 aaaaacaggt accgtataaa ttcatcggcg gcacaagcct gctgcaatca gcccatgtaa
      421 aggatttgct atcactcatg cgctgctcag caagtcatca tgatgaaatt gcatggataa
      481 gatatttaac cttatttccg aaaataggtg atataactgc actgaggatc attgaagaga
      541 tgaaaaaggc accgctcttt aatgaggcaa tagaaatact cagagacaac aagaaaatta
      601 tgcccgagat acttgcaggc ccgcacaagg ttatagaaaa atgggctgtg ccccatgatg
      661 caataagggc aggtattgat gtattggaac ctcttctaaa aaccaaatac agcaagtggg
      721 atactagaag aaaagatttt gatttactgg caagacttgc aaagaggcac aaaaatttac
      781 tgtcctttat agagaccttt accatggatc ccatctctac aagtgaggcc agcaggctcg
      841 aaaacgacga tgcagtcact ctcataacag tacacagcgc caagggtaca gaggccaaga
      901 cctgttactt aattaaagta gagcccggga tgtacccgca tgtaaggagc ctcggtaagc
      961 tggatgatga agaagaagag cggagagtgc tctatgttgc catgacaagg gcacaggacg
     1021 agcttattat tacaaggaac ctatcgcagg gcggcaggta taactttcac gggggctaca
     1081 gagcagaaca ttccagagac gtttcaccct attttcttga aagaatcccg gacaaccttg
     1141 ttgatacaag ctatgtgggt tttggcagct acggcatgca gaattccgat tttataaagc
     1201 cctgggacag agaaggatat taatatttat aagttgtaat ctgcaaatta cgctttagac
     1261 tttacctatg aggattattt taagcgtact aatcctaacc ctgctttttg ttctatccgg
     1321 ttacaactct gttaatgcca gatccggcag caataaatcc gcttattcca aatcaggaca
     1381 ccgaaaacag tccgcccagc agtttaaatc cggcaagcat tcgggtggaa agcactacaa
     1441 cagacagcaa aaatctcatt ataataatgg caaatattca ggtcatcata aatattataa
     1501 aggtcataaa agcggacatt atgccaagaa gcatcactac aaacatcata ataagcataa
     1561 aggaagcagt tacggtatct actatggcgg caataacctg agctttggct attacggtta
     1621 caacaggccg tactacaaac cttacggata ctatggctac ggttataagc catactattc
     1681 ataccctaca tactattact cctatgacag agggtactat gacaggaaat attcgcagga
     1741 cagatactat gacaatcaaa tttactctga agaaattgaa cccggacgag atgactccca
     1801 gtatgagtat ttagaagaaa aacctgctga gcagtactcg gaggagcgtt attcaaatct
     1861 caaagaagat accgaagatt actacccgga agaagaatac gaagatgaca gcggcgagcc
     1921 ggaatatgtg tacaaatgga tagataatga cggtacagaa cactacacca acaaccccaa
     1981 aaaagtaccg gaagagttta gagagactgt ggttaaactc gaagtctggt aagaatttca
     2041 cactaaccat tttatttttt ttcaattgat ttaatattct tctaagtttc cattctcaat
     2101 ttatttaatg tattaaatca ataatataaa tgacttcttt ttttgtacct ggaggattga
     2161 tatttttatc cctgttggga ttatttaagc ttaatgtgct tgcaggccta tctgcaaact
     2221 atatagaagt atattcgatc atagtactgg cctcttcatt tttaataggg ctttctttta
     2281 aaagaagcag gcttatttac tcaatcatta taatttcact tgcaggattt gtactttata
     2341 aagaatttgt aacagtaaat ctgaatctgt ttttactctc gctttgtctt ccactgaatt
     2401 tctttgttat ttacaacatg cacgaaagag gagttttaca gctatcattt tggaagtatt
     2461 ccattctcct tgtaattcag attcttcttt actactacat aagcagccac gaaaaagttt
     2521 ttgcagacaa tgtctttgat gtaagcacaa tagacaagcc ctttttcagt gaccttttac
     2581 tctccaaatt ttcttttgcc atttatataa gtgtcctgat ctactttctt gccgacttta
     2641 tatataaaaa ggatttctct gacagcgggt ttttctggtc atttcttaca actatcttca
     2701 tgttcagcga acagtttaat acctggaatc agattttttg tctcagcaca tcaggcctga
     2761 tacttcttgt aagctttgtg cttaggtcat atgacgtagc ctataaagac aaactcaccg
     2821 agcttccctc aagaagatca tttgatgaag ccatttcggg acttgggaaa gagtattcaa
     2881 taggtcttgt ggatatagat ttctttaaaa aattcaatga caggtacggc catgacgttg
     2941 gtgatcaggt actaaagatg gtagcctcaa gactatcaaa agtagggggc ggcggaaaag
     3001 cgtatagata tggcggagaa gagtttgcta tattatttgc aaatagagac aaagagcact
     3061 catatgagca tttagaaaat ttaagaaaag atatcgaaaa acagcagttt agcgtgcggg
     3121 gcaagaaaag gccaaagaaa aaacccaaga ttccggtatt tttcagcaag gacaaaactg
     3181 aaaagataac agtgagtatt ggtgttgccc aaaaaactga aaagttaagt gaagtacaag
     3241 atgtaatcaa agctgctgac aatgcgctct ataaagcaaa gaatgaaggc agaaacaggg
     3301 tttgtttaaa gtgaattttt agtttataaa ttgtaggata tatgaaagac tattttagca
     3361 gcggatttaa gattggaata ctcggcggtg gtcagcttgg aaagatgctc atacaggctg
     3421 caagctgctg ggatatagat atttatgtgc tcgaccccac tgaaggctgt cctgcatcaa
     3481 acattgccac aaaatttatt aagggtgatt ttaaagattt tgatgatgta tataattttg
     3541 gcaagcaggt cgatcttgtt acaatagaga tagagcacgt aaataccgag gcactactta
     3601 aactaaaaga ggaaggcaaa accatctgtc ccgaccccga tatcctaaaa atcattcagg
     3661 ataagggcaa acagaaaaac tttttcaaaa acaataacct tcgcaccgcc aactacaagc
     3721 tatactataa tcagcaaact attttaaatg acctggacta caaaaagctc gagaccccat
     3781 ttgttcaaaa ggcctgccgc tttggatatg acggaagagg tgtacagatt gtaaagaacg
     3841 aagatgatct ggaaaacctc tttgatggcc aatcagttat tgaagataaa gtttatataa
     3901 aaaaggaact ctcagtaatc gtagcccgca atcattctgg cgagattaaa tcatacgttc
     3961 cctttgagat ggaattcaat gaacaggcaa accttgtaga agatcttatt tctccagcca
     4021 gaattgataa cgaatcagcc cagcaagcta tcaagttagc agaacaaact ataaaaacat
     4081 ttggacttgt aggggtactt gccgtagaaa tgttcctgga taaggaaaat aacattctta
     4141 taaacgaagt tgccccaagg ccccataaca gcggacatca tacgattgaa acagcgctca
     4201 cttctcaata tgaacaacac ataagagcaa tccttgacct tccgcttgga agcacccagc
     4261 ttattagcag tgcggttatg gtaaacatat tgggagagga cggatttagc ggacaggtaa
     4321 gatatgaagg gttagataaa tgtattggta tcgatggtgt caaaatacat atttatggta
     4381 aaaagcagac taaaccgttt agaaaaatgg gacatgctac aataattgat aaagacataa
     4441 acgaagcaat taaaaaagct caaaggctta aacttaattt gagggcagtg tcatgaaaaa
     4501 accgctagtt agtataatta tgggatcgga ttcagatttg agtgttatgc aggaagcagc
     4561 acagtttctt gatgaaatgg ggattgcatc agaactcacc atagtatcgg ctcaccgtac
     4621 tcctgaaaga atgtttaagt ttgcaaaaac agctcacaca aggggtataa aagtaattat
     4681 cgccggagcc ggaggagcgg ctcatctgcc agggatggta gcttcactaa gcccacttcc
     4741 cgtgattggg gtgccaatta aatcatcaaa ctcaattgac ggctgggatt ctgttctttc
     4801 catactgcaa atgcccgctg gagtacctgt tgctacagtt gcacttaacg gagcaaggaa
     4861 cgcagggata cttgcagcgc agatcgtagg tacaggaaac aaaagaacac aaaataagat
     4921 ttcagaatat aaagattccc tggaaaaggg ggttgaaacc aaatacaaac gccttgaaaa
     4981 actgggattt aaacaatatt tattaaaata taaaaagtag gttgtactgt gtttactaag
     5041 aacagcatta caattatttc gtttgcttta attctatact tcaccgcact aactaactta
     5101 ctccatgcac aggtatacaa atgtgtggat agtaacggaa atgttctata cagcaattcc
     5161 ccctgccctc aaaatacaga ggaacaaaat tataagccaa gagagagctt tacaataaac
     5221 gatgaaacaa ctgacccgga aattgaagat ttaactgaga ctgaacaaac ctctcagaac
     5281 tttggcgcag gcgagctgga cataaatata gtacaggtaa gattatcttt acctgctcaa
     5341 caaataactg ccatacacaa gggagtccat ttcgtgtacg attattttaa agaagtatat
     5401 ggaataaccc cgggtacaga tatcaatatc aagatattcg gcaggcacgg agattttctt
     5461 gcataccaaa aaaggctaat tggaaagatt tattcagaat caggaatttt cagctcccga
     5521 acgggtgagg cgcttgttaa tgcaagcaaa taccgaaacg aagtggtagg aatatcaata
     5581 cacgaggcat gccatgcaat gattcagcag gccggttaca gaatacctgt ttgggtaaat
     5641 gaaggtattg ccgagtattt tgaaacaata gaaatatcag ataatcaggt acttttcaga
     5701 ccccagtatg acagacacag tgaaattgct tcgctgctta gtaaaaaaga gctgatatca
     5761 ctgctcgagt actttggtct ttcaaataat gtttggaaaa acagaaacct tgtagaagac
     5821 agaacaagcc gctcaattgc ttattctctg gtccattatc taatgtccag tgtcgaaggc
     5881 accgaatcta taaagaaaat cctcaaaaac tttagtgttt ccgacgaaac cccatcgatg
     5941 ttcgtagtaa atcaaagcta ttccggcggg cttaatttgc ttgagaaaaa ctggcatgaa
     6001 tacctaacac aaaccccttc aggacatgtt tttagtacaa atcagtagaa taagcttaca
     6061 ttccatataa cttttagtaa ttcataagtt tcggtcttga aatttattat tccatacata
     6121 tattttcagg gaaattagac taaaaaaacc ccgcgaggag atcaacatgg cagaaagtgg
     6181 tacgaataaa catgaatttc aggctgaggt aaaacagctt ttagatattg tagtacattc
     6241 tctttataca gataaagagg tttttgtaag agagcttgta tcaaacgcct cggatgcgct
     6301 tgaaaaacta agacacataa aacttactga aaaagacatc tttgatgaac agcttgactt
     6361 agaaattgag atcacaactg acgataaagc taacataatt acgatcaagg attttggtgt
     6421 aggaatgaca gaggatgagc ttgtagaaaa tttgggaact attgctcact caggctcaaa
     6481 agcatttttg gaagctgtaa aaaagggtga ggaaataaac agtaatttaa tcgggcagtt
     6541 cggcgtgggt ttttacagtg catttatggt tgcaaaaaaa gtagaggttt ttactcacaa
     6601 ctggaaagaa gatgcaaaac acctaagatg ggaaagtgat ggatccggaa gctatgaaat
     6661 atcagaagag ccgggacaaa gaaggggctg caagattgtt atccacctaa aagaagagga
     6721 taaggagttt gcagaacctg agaaaataaa aggcatttta acccgctatt ccagctttgt
     6781 tcagtttcca atcaaactta acggcgagca ggtaaacaca gtccaggcta tctggatgag
     6841 aaacaaaaat gagataaagg atgaagaata tacagagttt tataagtttc agggaaatgc
     6901 gtttgatgag ccaagataca cgcttcactt cagcgccgac gca
//
LOCUS       DBNGSV010000020         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1387955, whole genome shotgun sequence.
ACCESSION   DBNGSV010000020 DBNGSV010000000
VERSION     DBNGSV010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1387955"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC159U_00910"
     CDS             complement(<1..373)
                     /locus_tag="AC159U_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3335649.1"
                     /translation="MKAIVFTQYGPPEVLKIVEVDKPVPKENEVLIKVHAAVVSSADC
                     TFRRGEPYFARLYFGILKPRTTILGTEFAGQIEAVGKDVKNFTIGDKVIGAPGAAFGA
                     HAEYIAIAEDGALALMPSNLSY"
     gene            complement(370..849)
                     /locus_tag="AC159U_00915"
     CDS             complement(370..849)
                     /locus_tag="AC159U_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335650.1"
                     /translation="MEKDYLINSITQAFSEITYPGDDDLTVHPLGLDEDFYDSIKGKT
                     WQQLDSQLLRYHHDCIGVLTARGFQYYIAAFLIEDLKSGSPITDQMFWIFSQAVIEDR
                     SPIIGISGKAWFDERISMLTPDQKSCLVWYFTNIKEEQTEDDCAAIDIVIEKIKEIK"
     gene            complement(1025..1240)
                     /locus_tag="AC159U_00920"
     CDS             complement(1025..1240)
                     /locus_tag="AC159U_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335651.1"
                     /translation="MVGRVLFLISAIGMILLGASIFRMPEWYDNTFNIGFSNNGFGEI
                     LAAVLILVGLILVVSIIRIALNNINES"
     gene            complement(1323..2330)
                     /gene="obgE"
                     /locus_tag="AC159U_00925"
     CDS             complement(1323..2330)
                     /gene="obgE"
                     /locus_tag="AC159U_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3335652.1"
                     /translation="MKFIDEANISVVAGRGGDGCVAFRREKFVPLGGPSGGDGGKGGN
                     VILFADANINSLLDFRYKKIYKAQRGANGRGKNQHGKYGQDIIVPLPVGTLVKDADTG
                     EVIYDLKQQGESYVIARGGIGGRGNARFASSVNQAPREFEEGTEGEQRNLDLELKLLA
                     DIGIVGFPNAGKSTLISRISAAKPKIADYPFTTLIPNLGVVEYGEHKTFVVADIPGLI
                     EGAHTGAGLGINFLKHIERTSILLYLIDISPVDNKDPVSQFEILQGELNKFSSELLEK
                     PMIIALNKIDTLSKDEQTEIVEVFRNRGLAVRLLSAFVGTGVKELVAELGQVYEKNKE
                     I"
BASE COUNT          751 a          570 c          356 g          769 t
ORIGIN      
        1 catagctaag attggaaggc ataagtgcaa gtgcaccatc ctcggctatg gctatatatt
       61 cagcatgggc tccaaaagca gccccgggtg ctccaatgac tttgtcaccg attgtaaaat
      121 tcttaacatc cttgcctaca gcttcaatct gtccggcaaa ctcagtaccc aatatagtag
      181 tccttggttt taaaattcca aaataaagcc gtgcaaaata tggttcaccc cttctaaatg
      241 tacaatcagc actggatacc accgccgcat ggacttttat aagcacttca ttttcttttg
      301 gtacaggctt atctacttct actattttta aaacttccgg cggtccatat tgtgtaaata
      361 caatcgcttt cattttattt ccttaatttt ctcaataaca atatcaattg cagcacaatc
      421 atcctctgtt tgttcttctt taatattagt gaaataccac accaggcatg atttttgatc
      481 aggtgttaac atacttatcc tctcatcaaa ccatgctttt ccactgatac caataatggg
      541 ggatctgtcc tcgataactg cctgtgaaaa aatccagaac atctgatctg taattggtga
      601 accggatttc aggtcttcaa ttaggaatgc cgctatataa tattgaaatc ctcttgcagt
      661 cagcacccct atgcagtcat gatgatatct gagtagttgg gaatccagct gctgccaggt
      721 tttacctttt atagaatcat aaaagtcctc atccaatcca agtggatgaa ccgtgaggtc
      781 atcatcaccc ggataggtaa tttcagaaaa ggcctgggtt atactgttta taagataatc
      841 tttttccatc tctaatgaga ccaaacataa atatggatta attatataga aaacacaaaa
      901 agaatggata ataaaaataa tacaatatta attttcgctg tactatctgt attatatgga
      961 catcaaggcg aattagtaag tattaatttg ccattgcata ctgacaaaca gatcaaacat
     1021 acaatcacga ctcattaata ttatttaatg ctatccttat aattgatact accagaatta
     1081 aacccacaag aataagaact gctgccagga tttcaccaaa accattattg ctaaaaccta
     1141 tattgaatgt attatcatac cattcaggca tcctaaaaat tgaagcacca agcaatatca
     1201 tccctatagc gcttattaag aataatactc ttcctaccat ggtcagatct aaaaaaactc
     1261 ctgataaaaa ctaaaatatt gttatattat aaccgacccc gatactttgt gccaatatta
     1321 ttttatattt ctttattttt ttcataaacc tgcccaagtt ctgcaacaag ctcttttaca
     1381 ccagtcccta caaaagcaga taacaacctg accgcaagcc ctctatttct aaaaacttct
     1441 actatttctg tttgttcatc cttacttagc gtatcaatct tatttagggc aataatcatt
     1501 ggtttttcta gtaactctga agaaaactta tttaattctc cctgaagtat ttcaaactgg
     1561 ctcaccgggt ctttattgtc aacaggggat atatctatta aatacagcag gatcgatgtc
     1621 ctctctatgt gctttaaaaa gttaattcct agccctgcac cggtgtgcgc cccttcaatc
     1681 agacccggaa tatctgcaac tacgaatgtt ttgtgttccc catactctac aacaccaaga
     1741 tttggtatga gcgttgtaaa aggataatca gcaatcttgg gctttgcagc agatattctg
     1801 gatattagag ttgatttccc cgcgtttgga aaccctacaa tacctatatc agcaagaagc
     1861 ttaagctcca ggtcaaggtt tctctgctcc ccttctgtcc cctcttcaaa ctcccttggt
     1921 gcctgattaa ctgagcttgc aaacctggca tttcccctgc ccccgatgcc gcctcttgca
     1981 ataacatagg actctccttg ttgtttaaga tcataaatca cttcaccagt atcggcatct
     2041 ttaacaagag tcccaacggg aagaggcact ataatatctt gtccatattt tccatgctgg
     2101 ttctttcccc tgccgtttgc tcccctctga gccttataaa tcttcttata tctaaaatcc
     2161 aataaagagt ttatatttgc atcggcaaac aatattacgt tgcctccttt gccgccatca
     2221 cccccactag gaccgcccaa aggtacaaac ttctcccttc taaacgctac gcatccatcg
     2281 cccccacgcc cggcaacaac acttatattt gcttcatcaa taaatttcat aatactttta
     2341 gtaaaactaa tatatctaaa cccttgacac tacacgggct tattaataat ataaagaacc
     2401 gatgagaaaa ttattactat taaatttaat taccttaaca ttaatt
//
LOCUS       DBNGSV010000021        15061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_418084, whole genome shotgun sequence.
ACCESSION   DBNGSV010000021 DBNGSV010000000
VERSION     DBNGSV010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_418084"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="AC159U_00930"
     CDS             <1..1305
                     /locus_tag="AC159U_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3335653.1"
                     /translation="ITDERTNSLIVIAYPDDMPKIKAEIERLDVEVDEPDHGIYVIRL
                     KNGDSEQIVNVLNSLISGAANTSSRTSRGFAATRESQQQQDQGRGVQRTSASGAIAEF
                     SLEDIRITSDISTNSIIVVGSRREFDAIKRVIDELDIRRRQVFVEAAILEVSLDQLKS
                     LGTNLSIGFTINDDNLGFGGTNLPGVPSLLGAAADSENTVSLVGSLSGMFLGVVGEEV
                     DPDGSGPIPPIPSFSALFQALTSVTDVNILSTPSIVTTDNEEAEIVVADVIPFPTGST
                     VGDSGVTVQTIDRQPVGIRLAITPQIGDGDFLHLELQTEVSSVRDAPTDLNTEDFGIA
                     TTTRTASSSVVVKNGQTIVIGGLVQDRESNLENRVPGLGSLPILGNLFKFKSKSSSKI
                     NLMILLTPRIIEDERDMKLILEDRQKRNMLLQQRGFDTEGVY"
     gene            1310..2986
                     /gene="gspE"
                     /locus_tag="AC159U_00935"
     CDS             1310..2986
                     /gene="gspE"
                     /locus_tag="AC159U_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR3335654.1"
                     /translation="MRSLQEILVDRNPELGSRIKDILTASEDSAEEAIIRSNVLNDKQ
                     IMEAFSEYYELPFRVNIFEKEIDPELAQLIPIAFAKKNRIIPLNKDNNKVYVAVSPPI
                     DLYSLDEVKSLFNCEVEPFLSPSHVVLDCINKVYERDREVTENIEGDVTGISEADFQE
                     PKDLLEAEDEAPIIRFVNSLLFQAVKEKASDIHVECFEKDVMIRFRKDGMLHKITEIP
                     KKLQSSVISRLKIMAELDIAERRKPQDGRIRVKVAGRDVDVRISTVPTSWGESVVMRL
                     LDRSSVLLSLEDLGVTGQKLTDINNFIKRPHGIILVTGPTGSGKTTTLYASLVRINSP
                     DKKIITIEDPVEYQLQGVNQIQVNTKVNLSFANGLRSILRQDPDVILVGEIRDRETAD
                     IAIHASLTGHLVFSTLHTNDSASAITRLIDMGIEPFLVSSSLMAVVAQRLVRHLCNHC
                     KEEYTPTDEELMKIGIPRASVLNDVLFRSTGCEQCLDTGFAGRSGIFEILKMDDEIRN
                     LTLQSTDSSTIKKKSVERGLITLRGDGIAKILMGQTSIDEVLRVTEQDEV"
     gene            3028..4239
                     /gene="gspF"
                     /locus_tag="AC159U_00940"
     CDS             3028..4239
                     /gene="gspF"
                     /locus_tag="AC159U_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR3335655.1"
                     /translation="MPVFKYKAINNQGNTVDGTIDAESIKTASDKLKKDGFFPSSIDE
                     VQKGGERNRLSLFKGVSTSELAISTRQFSTLISAGLPLEASLTTLSEQTEDKNLSQVL
                     SQVRDKVSEGGSLANSLKDHKNVFSDIYTSIVRAGEASGNLDVVLLRLADFLEKQAAL
                     RSKVRGAFVYPIFMFVIGSGVLVFMMTFVIPRITKIFEESKKALPFMTVLLINTSNFI
                     VQYYYLIFIFFIGIAYILYRYVQTDKGKAQKDKLSLKIPIFGKINRMVILARVTRTLG
                     TLLSSGIPLLDALRIGGEVSGNQVYIDSLNSVSENVKEGTSLARPLEQSGVFPPMMTR
                     MIAVGEQTGELEEMLDKVADSYDQQVETTLSALTSLLEPIMIVVMGGIVGFIVMAILL
                     PIFDITSTISN"
     gene            4258..4674
                     /gene="gspG"
                     /locus_tag="AC159U_00945"
     CDS             4258..4674
                     /gene="gspG"
                     /locus_tag="AC159U_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR3335656.1"
                     /translation="MNSSSGFTLIEILVVVIIIGTIASIVGVNIVQKLEEAKIDTTKI
                     QITNLESGLKLFKIKNGFYPETDQGLEALVSAPSVGREVKHSTGFIDGDKIPLDGWGS
                     EFEYIGPDQTGDKSYEIISPGPDAQLQTEDDISSKD"
     gene            4677..5186
                     /locus_tag="AC159U_00950"
     CDS             4677..5186
                     /locus_tag="AC159U_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3335657.1"
                     /translation="MRDEKGFTIIEIIIVVVILSSFLLVAVPRLDNFTEGNLKSGSRN
                     LSTTIRYLYSEAAFKKKIHKLAFDIDEGEYWVEVLEVDEYEVSTDPFLKKRKLPNNVS
                     FKDIVTLRSLGISTEGKDEFILFLPSGFVEPVVIHLESNSGDVYTLATKPYTGATAVF
                     DEYKEILIQ"
     gene            5158..5592
                     /locus_tag="AC159U_00955"
     CDS             5158..5592
                     /locus_tag="AC159U_00955"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3335658.1"
                     /translation="MNTKKFLFNKIMTVKKKYIKPNCNRQSGFTLLEVLVAVVILGSS
                     LAVLLGTVNRNLVLTSDSKSLSIARMLAQDKISEVELEGYPENKNEEGEFEDFPDFKW
                     YLSVAPLEIASLETDIKIVKLLITWDNDQKDFEITYAMANYE"
     gene            5570..6250
                     /locus_tag="AC159U_00960"
     CDS             5570..6250
                     /locus_tag="AC159U_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR3335659.1"
                     /translation="MQWQTTSKGFTLIEVMIALFIGSLIFGVIYSSFFQIMKSKDIAE
                     NELELYHEARVILSRITKDLNSVYPRGNIFVGKSNYISAEPYFEGGLENDNSWIRFTS
                     LSRSPSYNKSDSDQAQINYYVQKIEDRNSDRDDQLYSLFRRENPYIGGNEEGVNYSIS
                     DRIVLFRLNYLSGESIDTEDSFSDIWNTNENLAMPRAVEISFVLRGPEQKDVEFKNLV
                     YLQLSKFN"
     gene            6251..7333
                     /gene="gspK"
                     /locus_tag="AC159U_00965"
     CDS             6251..7333
                     /gene="gspK"
                     /locus_tag="AC159U_00965"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HIR3335660.1"
                     /translation="MKNKKSQSGVVLIVVLIAIAILTTVVVDLVYFTHVDAQIAANSK
                     DDIQTSYIARSGVNVVAGTLKNRSLEDLDSVVSLYTGDDLNAKGRWAITVPNFPVGQG
                     VVTIRVIDERSKINLNTLVNQNSNRIDLQVKEQLAELFDQLGIESSKYDRFISSLINW
                     LDRDLEGSQNNQDPSGADGGFYLSEENSYAIKDGPLDSLEEIRLIDGMDTEFYEEVKD
                     YLTVYPVDKRINFSTAPKVVLLAALQAATVSAVEGQGSFADIELNDDIAQRIADEIIS
                     ERDNEPIITRNEITQIIQNIDPTLRINSGIAGVSVSSGTSDTFYVNATGILGADNPTK
                     KIVDAVIRKQGSGNNSGVKIISWKEN"
     gene            7318..8469
                     /locus_tag="AC159U_00970"
     CDS             7318..8469
                     /locus_tag="AC159U_00970"
                     /inference="COORDINATES: protein motif:HMM:NF040105.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3335661.1"
                     /translation="MERELEKLKTVINELKSEFYERDDLIEGAVCALLTSSHVIIIGP
                     PGTAKSQLANELCSRVKGANYFQWLLTKFSTPEEIFGSVSLKGLENDEYRRVLTNKLP
                     EAHVAFLDEIFKSSSSILNSILTVINERIYFNGTEKINTPLISLFGASNELPSDEDEL
                     DALYDRFLLRFVVGYIEEDFRFLKMIGNKDDVGKNTSITIKELQNLQHGVQKVVIPQT
                     IYKLVLRLRTELSSKNIILSDRRYKSAISLIQAKAFINGRETACEEDIKILENVLWKD
                     PAQISDIKTTIHSVLFGFREQLKELYIQAKEIEAYSKQKWEDEETKIKANIEAQTKLK
                     MLINKVDVLSEELTDRGISTNEVSEIKVQIEDVKKEILNSLIDPKQAVI"
     gene            8510..8596
                     /locus_tag="AC159U_00975"
     tRNA            8510..8596
                     /locus_tag="AC159U_00975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8544..8546,aa:Leu,seq:cag)
     gene            8843..8980
                     /locus_tag="AC159U_00980"
     CDS             8843..8980
                     /locus_tag="AC159U_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR3335662.1"
                     /translation="MDSKDYVYIMIDKTIGYILPKKIFSSPSDIDTFLKQINQYKELK
                     Q"
     gene            9036..9974
                     /locus_tag="AC159U_00985"
     CDS             9036..9974
                     /locus_tag="AC159U_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3335663.1"
                     /translation="MARKPRIHVPGGFYHVILRGNAGQDIFYSVEDTKYFESLISEGI
                     ERFGHRVHTYCWMKNHVHMVIQVANIPLSKIIQNISFRYTLYINKKYDKTGHLFQGRY
                     KAILIDPQSYLLQLVRYINLNPLTANIVNKPEEYRWSSYRAFVGKCRTDWLTLDYILK
                     LFSDNKSEAIEQYQEFVQDRDEADLNIDFSSGNKHGYVLGDDLFAEKVIQASQSIDKN
                     RMSIEKIITTACRSFDISKDQLISNDRARDLGRIRTIIGYLIIEYGSSSLSEYARYIK
                     RDISTISGAVARYRKKLLSQSEEQIYMAKLEKELGV"
     gene            10225..10671
                     /gene="mraZ"
                     /locus_tag="AC159U_00990"
     CDS             10225..10671
                     /gene="mraZ"
                     /locus_tag="AC159U_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3335664.1"
                     /translation="MFRGRYEHTMTDKGRVSVPSRFRDLLKSKYEDETLIITNFNKCL
                     AAYPLQEWYEIENKVAELPQFKQEVISFLRYLMGGAVECPIDSQGRILIPQSLRSYAS
                     LDKDVVLIGMLNRFEVWSREVWGSEESVKSYEEFQKSSEILAGQGI"
     gene            10673..11638
                     /gene="rsmH"
                     /locus_tag="AC159U_00995"
     CDS             10673..11638
                     /gene="rsmH"
                     /locus_tag="AC159U_00995"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3335665.1"
                     /translation="MLNTAMKIEQNNIHVSVLKSEIGQYLRPFGGGLYIDATLGFGGH
                     TLEILERAGYKAKLICFEVDTDAIAITSELLSSYSENVTIVNRNFSELKSVLDQSGIE
                     KVDGIVADLGLSSYQLEQSGRGFSFQKDEVLDMRADMDSDLTAFDIINEYSAQDLAEI
                     FWEYGEERFSRQIAKAIVRKREEGEISTTLQLCDIIEKTIPQRFKPKKINCSTKVFQA
                     LRVCVNNELDNLSLFIETAIERLKVGARIAIISFNSLEDKIVKRAFAKYTSPCTCPSD
                     LPVCSCGNKPAIKLINKRAISPGEAEIRSNPRSRSARLRVAEKIL"
     gene            11649..11969
                     /locus_tag="AC159U_01000"
     CDS             11649..11969
                     /locus_tag="AC159U_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335666.1"
                     /translation="MREKLKQKVSSREILILILFIVGFFMFYLHTKIELIEVGYEISD
                     NKNYKRSLSEKNQMLKAEFLNLKSPDRIEKLALKRGLIYPSQKDILYSGNNKDLSAYS
                     GKDE"
     gene            11962..13875
                     /locus_tag="AC159U_01005"
     CDS             11962..13875
                     /locus_tag="AC159U_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR3335667.1"
                     /translation="MNNNKYKSKLNLARNSDKPKWKLSCIAVFICFMFVLVAARSAQL
                     QLLRDDKACVVGKDQHEFEIELLPDRGAIKDAGGKDLAMSVTSYSIYANPLKIENKDQ
                     FVSKVSKTIDLKKDYIEKKINAQKSFVWLKRLASEEDKLSLQQLQLSGLGYIEEPKRV
                     YPYNHLAGQLIGFTNIDSKGIEGLEYLYDTYLSGKRDKLKLKKDGLGRVIFEHPSEIK
                     DDALGYDIVLTIDSHIQHIADNSLKKHVESFDADSGFAIVIDSEKGSVLAISSYPFLD
                     PNKFSDSSDLARKNLPVWKSFEPGSTLKPLLVAGAIEEKLINPNSKFDCENGRMKVGD
                     SIINDVHPYKILAVSDVISLSSNICASKIGVKLGKNRLYDYLHDFGFGKKTEIEIPGE
                     SSGLLLSSDSWGPVELATISFGQGISVSALQLANAFSSIANKGYLMKPYIVKEIVDSK
                     GNIIMRNIPEIRKRVISYETANMVADMLIEAVDNGTGKNAQVKHYKVAGKTGTAQIPD
                     PKNGGYYADKYISSFAGFAPIDPPSITVVVVVENSKNGFYGGEIAAPIFKEIVEKTLY
                     YMEISPEGKGLDNMIMPNFEGKSRREILRWSETEGVKVNLSGKGFAIDQSPKPGEIIN
                     SKEVCSVKLDQTI"
     gene            13872..15023
                     /locus_tag="AC159U_01010"
     CDS             13872..15023
                     /locus_tag="AC159U_01010"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR3335668.1"
                     /translation="MNLSDLLQGYECKLINGDKFKVVESIEYSSREVNKNSLFVAIKG
                     EKLDGHRYISDAVNRGAGVIVVEDSAGEKQPESVTIVEVKDSLDALAYLSSKYYGDPT
                     KDIRITAVTGTNGKTTITYILESICTGENQIVGVIGTINYRYNNRQYPSSLTTPLSKE
                     LQHMFFEMKESGVQHVVVEASSHALDKKRLDYSNIDCAVFTNLTQDHLDYHKDIGSYF
                     LAKKRLFSNVLAHSCKDNKIAVSNYDDPYGKKIVEGFDGNILFYSIDNPVADIYAKDI
                     QISTDGIKARAVTPAGEVEINSELLGRHNLSNILASVACAVGAGVSADDIAKGIATLK
                     NVPGRLQRVSNSKGINIFVDYAHTPDALGNVLVSVKSLCRGRLIVVFGC"
BASE COUNT         5117 a         2356 c         3246 g         4342 t
ORIGIN      
        1 atcacagatg aaagaacaaa ttcacttata gttattgcct accctgatga tatgcctaaa
       61 attaaggcag aaattgaaag gcttgatgta gaagttgatg agccagatca tggaatatat
      121 gtgataagac tcaaaaacgg tgattcggaa cagattgtga atgttctaaa ttcattaata
      181 tcaggcgctg caaatacatc aagtagaaca tccagagggt ttgccgctac cagggaaagc
      241 cagcagcagc aggatcaggg aagaggagta caaagaactt ctgcttccgg agctattgct
      301 gaatttagtt tagaagacat aagaataaca tccgatattt ctacaaattc aataatcgtc
      361 gtaggttcaa gaagggagtt tgatgcaata aagagggtaa ttgatgagct tgatatacga
      421 agaaggcagg tatttgtaga agcagcaata ctcgaagtca gccttgatca gctaaagtca
      481 cttggaacaa accttagcat cggttttaca ataaacgatg ataacctggg gtttggaggt
      541 actaaccttc ctggtgttcc aagtctttta ggtgctgcag ctgacagcga gaatacagta
      601 tcacttgttg gaagtctgtc tggtatgttt ttaggagtcg ttggagagga agtagatcct
      661 gatggttctg gtccaattcc gccaatacca tcatttagcg cactttttca ggcacttaca
      721 tctgtaactg atgtaaatat tctttctact ccaagcattg ttactacaga taatgaagaa
      781 gcagagatcg tggtagctga tgtgattccg ttccccactg gtagtacggt tggagacagt
      841 ggggttacag tccagactat cgacagacaa cctgtgggta taaggcttgc aatcactccg
      901 caaataggtg atggagactt tttgcatctt gagcttcaga ccgaggtatc atctgtaaga
      961 gatgcaccga ctgatcttaa caccgaagat tttggtatcg ctacaactac aagaactgcc
     1021 agctcttcgg tagttgttaa aaacggtcag accattgtaa ttggcggctt ggttcaggac
     1081 agagagagta atttggaaaa cagagtgccc ggacttggaa gtcttccgat acttggaaac
     1141 ctatttaaat ttaaatcaaa atcaagtagc aaaatcaatt taatgatcct tctaacccca
     1201 agaattatag aagatgaaag agatatgaaa ttaatacttg aagataggca aaaaaggaat
     1261 atgctattac agcaaagggg ttttgataca gaaggagttt attgattgaa tgaggtctct
     1321 gcaagaaata ctggtagaca gaaatcctga gctaggatca agaataaaag atatattaac
     1381 agcaagtgaa gacagtgcag aagaagccat aataaggtct aatgtcctga atgacaaaca
     1441 gatcatggag gcgttttcag agtactatga gcttccattc agagttaata tttttgaaaa
     1501 agaaattgat ccggaacttg ctcagcttat acccatagca tttgccaaga aaaacaggat
     1561 tatccctcta aataaagata ataataaggt ttatgtagcg gtttcacccc ctatcgatct
     1621 atactctctt gacgaggtga aatctctatt taactgtgag gttgaacctt ttctttcacc
     1681 ttctcatgta gtgcttgatt gtataaataa agtatacgaa agggacagag aagttacaga
     1741 gaatattgag ggagatgtaa ccggtattag tgaagcagac tttcaggagc caaaagacct
     1801 gttggaagct gaagatgaag ctcctataat taggtttgta aactcgcttt tatttcaggc
     1861 agtaaaagag aaggctagtg atattcacgt agagtgtttt gaaaaagatg taatgatccg
     1921 ttttagaaaa gacgggatgc ttcataaaat taccgagata cccaaaaaac tacagtcctc
     1981 agtaatatcc agactcaaga tcatggcaga gctcgatatt gccgaaagaa gaaagccaca
     2041 ggacggcagg attagagtaa aggtagctgg aagggatgtt gatgtaagaa tttcaacagt
     2101 gcctacatcc tggggtgaga gcgttgtaat gaggcttctc gaccgctcat ctgtgctcct
     2161 tagtcttgaa gacctggggg taaccggcca gaagcttaca gatataaata attttataaa
     2221 aagacctcac gggattattc ttgttacagg gcctacggga agtggtaaga caaccactct
     2281 ttatgcctca cttgtaagaa taaattcccc tgataaaaaa attattacaa ttgaagatcc
     2341 tgtagagtat caacttcagg gagtaaacca aattcaggta aacaccaaag ttaaccttag
     2401 ttttgcaaac ggactgagat cgatattaag gcaagacccg gatgtgatac tggtgggtga
     2461 gataagagac agagaaactg cagatattgc tattcatgct tctcttaccg gtcatttggt
     2521 tttttccacc ctgcatacaa atgattcggc aagtgcaatt acaaggctca ttgatatggg
     2581 tatcgagccg tttttggtat catcatctct aatggctgtg gtagctcaga ggctagtaag
     2641 gcatttatgc aaccactgca aggaagaata tacaccaaca gatgaagagc ttatgaaaat
     2701 tggtatacca agagcatcgg tacttaatga tgtattattt agatcaaccg gctgtgaaca
     2761 atgtctggat accggttttg caggtagaag tggaatattt gagatattaa aaatggatga
     2821 cgaaatacga aatcttactt tacaaagcac agattccagt acaattaaga aaaaatcagt
     2881 cgagagggga cttataacac tcaggggaga cggtatagct aaaatactga tggggcaaac
     2941 atctatagac gaagtattaa gggttaccga acaggacgaa gtttaaactt tcttttttta
     3001 gatattccat taacattctt aacttatatg ccagtattta agtataaagc tattaataat
     3061 cagggaaata cggtagacgg cacaatagat gctgaatcta taaagactgc ttctgataag
     3121 cttaaaaaag acggattttt cccttcttca attgatgaag ttcaaaaagg cggcgagcga
     3181 aacagattat cgctttttaa gggtgtgagt acatctgagc ttgcaatttc aacaaggcag
     3241 ttttccacac tcatttctgc ggggctgcct ttagaggctt cactaaccac actatcggaa
     3301 caaaccgagg ataaaaactt aagccaggtt ctctctcagg tacgagataa agtaagtgaa
     3361 ggcggctctc ttgctaattc actcaaggat cataaaaacg ttttttctga tatttataca
     3421 agtattgtaa gggcaggtga ggcaagcgga aatcttgatg tggtacttct taggcttgct
     3481 gattttttgg aaaagcaggc tgcactaaga tcaaaggtaa gaggcgcgtt tgtttacccg
     3541 attttcatgt tcgttatcgg aagcggagta ctggttttta tgatgacatt tgtgattcca
     3601 agaattacta aaatttttga ggaaagtaag aaagcacttc cttttatgac tgttttatta
     3661 atcaacacaa gtaattttat agtacagtac tattatctga tttttatatt ttttattgga
     3721 attgcttata tattatatag atatgttcag acggataaag gtaaagcgca aaaagacaag
     3781 ctgtccttga aaatcccgat atttggaaag ataaacagga tggttatact agcaagggtt
     3841 acaaggactt tgggaacttt actatcaagt ggcataccac tactcgatgc cctgagaata
     3901 gggggtgaag tatcgggcaa tcaggtatat attgattctc tcaatagtgt gagtgaaaat
     3961 gtaaaagagg gtaccagtct tgcaaggcct cttgagcaga gcggagtatt tccaccaatg
     4021 atgaccagga tgatcgcagt aggagaacag acaggcgaac tagaggaaat gcttgataag
     4081 gtagcagata gctatgatca gcaggttgag actacactct cagcacttac gtctcttctt
     4141 gaaccgatta tgattgtagt catgggtggt atagtagggt ttattgttat ggctatttta
     4201 ttgccaattt ttgatattac atcaactata tctaattaat ttagggggta taaataaatg
     4261 aattcatcat caggttttac gttaattgaa atattggtag tggttatcat tataggcact
     4321 attgcttcga ttgtcggagt taatattgtg caaaaattag aagaagcaaa aatagatacg
     4381 actaaaattc aaatcactaa cctggaatcc ggactaaaac tatttaaaat aaaaaacgga
     4441 ttttatcctg aaactgatca aggcttggaa gctttggtat ctgcaccctc tgttggtaga
     4501 gaggttaaac attcaaccgg ttttatcgat ggagataaaa taccccttga tggttgggga
     4561 agtgaatttg agtatatagg tcccgatcaa actggagaca agagttacga aataatctca
     4621 cccgggccag atgcgcaact tcaaaccgag gatgacatat ccagtaaaga ctaacaatga
     4681 gagatgaaaa aggctttact attattgaaa taataatagt tgtagttatt ttgagcagtt
     4741 ttcttctggt agcagtacca aggttagata attttactga aggtaacctt aaaagcggct
     4801 caagaaatct gagtacgact ataaggtatt tgtatagcga agctgcattt aaaaaaaaga
     4861 ttcataagct tgcatttgat attgatgagg gtgaatattg ggtagaagtc ttagaggtag
     4921 atgaatatga ggtatcaacc gacccatttc ttaaaaagag gaaacttcct aataatgttt
     4981 catttaaaga tattgttaca ctaagaagcc ttgggatttc taccgagggc aaggatgaat
     5041 ttatactatt tctaccctca ggatttgtag aacctgtagt gattcatctg gaaagtaatt
     5101 caggggatgt ttatactcta gctacaaaac cttatacagg tgcaactgct gtgtttgatg
     5161 aatacaaaga aattcttatt caataaaata atgacagtaa aaaaaaagta tattaaacct
     5221 aattgtaata ggcaatctgg atttactctt ctcgaagttc ttgtagcagt tgttattctt
     5281 ggctcttcac ttgctgttct actgggtact gtgaacagga atctggtatt aacatctgat
     5341 tcaaaaagtc tctctatcgc aagaatgctt gcacaggata aaatttcaga agtagagctt
     5401 gagggttatc ctgaaaacaa gaacgaggag ggtgagtttg aggattttcc ggattttaaa
     5461 tggtatttat ctgttgcacc attagaaatt gcatcacttg aaacagatat taagatagta
     5521 aaattactaa taacatggga caatgatcaa aaagactttg aaattaccta tgcaatggca
     5581 aactacgagt aaaggcttca ctttaataga agtaatgatt gcactattta taggttctct
     5641 tatttttggt gttatctatt cttctttttt ccaaataatg aagtcaaaag atatagcaga
     5701 aaatgagctt gagctttatc atgaagcaag ggtaattcta tcgaggatca caaaagacct
     5761 aaactcggtt tatccaagag gtaatatctt tgttggtaaa tcaaactata tttcagctga
     5821 gccatatttt gagggtggtc ttgaaaatga taattcatgg atcagattta cctcactttc
     5881 caggtctcca agttataata aaagtgattc cgatcaggca caaataaatt attatgtaca
     5941 aaaaatagaa gatcggaatt cagatagaga tgatcagctc tattcactgt ttagaaggga
     6001 aaatccgtat attggaggaa atgaagaagg agtaaattat tcaatctctg atcgtatagt
     6061 actgtttagg cttaactatt taagtggaga atccattgat actgaagatt cattttcgga
     6121 tatctggaat acgaatgaaa atcttgcaat gccccgtgct gttgaaataa gctttgtact
     6181 acggggtcct gaacaaaaag atgtagagtt taaaaaccta gtatatttac agttatcgaa
     6241 gtttaattag atgaaaaata aaaaatcaca gagcggagta gtgcttatag tagtacttat
     6301 agcaattgcc atacttacta cggttgttgt tgatcttgtg tattttaccc atgtggatgc
     6361 acagattgct gcaaattcta aggacgatat tcagacaagc tacatagcaa ggtcgggggt
     6421 gaatgtggtt gccggtactt taaagaacag atcacttgaa gatttagaca gtgtggtctc
     6481 cctttacact ggtgatgatc ttaatgctaa agggcggtgg gccataactg tgcccaattt
     6541 ccctgtaggc cagggggtgg taactataag ggtgatagat gaaagatcta aaataaatct
     6601 aaatacactt gtaaaccaaa attctaacag gatagacttg caggtaaaag aacagcttgc
     6661 cgaattattc gatcaattgg ggatagaaag ctcaaaatac gacagattta tttcaagtct
     6721 tattaactgg cttgaccggg atttggaagg ttctcaaaat aatcaggatc cgtcgggtgc
     6781 ggatggaggg ttttatttat ctgaggaaaa ttcatatgct ataaaagacg ggccgttgga
     6841 cagtcttgaa gagatacgcc tgatagatgg aatggacaca gagttttatg aggaagtgaa
     6901 ggactatctt accgtatatc ccgtggataa aaggataaat tttagtacag caccaaaagt
     6961 tgttttactg gcagccctgc aggcagcaac ggtgtctgct gtggaaggtc aggggagttt
     7021 tgctgatatt gaactaaatg atgatattgc ccaaagaata gcagatgaga taattagtga
     7081 aagagacaat gagcccataa ttacgagaaa tgagattact caaataatac aaaatataga
     7141 tccaacactt agaattaatt caggtattgc gggggtatca gtctcaagcg gtacaagtga
     7201 tacgttttat gtaaatgcta cgggtatttt aggtgcagat aatccaacaa aaaaaatagt
     7261 tgatgcagtg attagaaaac agggatcagg aaataacagc ggggtgaaaa taatatcttg
     7321 gaaagagaat tagaaaaact aaaaactgta ataaatgaac ttaaatcaga attttatgaa
     7381 cgagatgacc tgattgaagg agcggtttgt gcactactta cctcaagtca tgtgattatc
     7441 ataggccccc cgggaacagc gaagtcacag cttgcaaatg aactttgttc gagggtaaaa
     7501 ggtgcaaatt attttcagtg gctccttacc aaattttcca cccccgaaga aatatttgga
     7561 tccgtaagtc taaagggact tgaaaatgat gagtatagaa gggtgcttac caataaactg
     7621 ccagaggccc atgtagcctt tcttgatgaa atattcaaat caagctcatc aatcttaaat
     7681 tcgatcctta ctgtaattaa cgagaggatt tattttaacg gcaccgaaaa aattaatact
     7741 ccgttaatct cattatttgg agcaagtaat gagctgccat cggatgaaga tgagctggat
     7801 gccctttatg acaggttcct tttaagattt gttgtgggat atatagaaga agatttcaga
     7861 tttttaaaga tgataggtaa taaagatgat gttgggaaaa atacaagtat aactattaaa
     7921 gaactacaaa atttacagca cggggtacaa aaagttgtaa ttccacaaac gatttacaaa
     7981 ttagtattaa ggctaagaac tgaactctct tcaaaaaata tcattctttc cgacagaaga
     8041 tataaaagtg ctatatcact tatccaagca aaagcattta taaatgggag agaaaccgct
     8101 tgtgaagagg atattaaaat cctggagaac gtattgtgga aagacccggc tcaaatatcg
     8161 gatattaaaa ccacaataca ttcggtattg tttggattta gggagcagtt aaaagaactc
     8221 tatatacagg caaaagaaat tgaggcttat tcaaagcaaa aatgggaaga tgaagagact
     8281 aaaataaagg ctaacattga agcccagact aagcttaaaa tgctcataaa caaggttgat
     8341 gtattgtctg aagagcttac ggacagagga atttcgacta atgaagtaag tgaaattaag
     8401 gtacagattg aagatgtaaa aaaagagata ctcaattcac taattgaccc taagcaagca
     8461 gtaatttagc aatctaatta ttcttgttat tattagcaag aaattttcag ccgaagtggc
     8521 ggaactggca gacgcgcagg actcagaatc ctgtgagtgt gagctcgtgg gggttcgaat
     8581 ccccccttcg gcaccattaa ttaccttaat atcccattta ataaccaaaa taccaaagcc
     8641 tgaccccact ttgtgctaaa ataatacttg cattttattt tggtaagtaa tatgatctac
     8701 ttataacttt agtattgtgt ttaatgcaag ataaaaaata tttttagtgg ggggaacatg
     8761 atgaaatatt ttctatcttt agtgtcatta cttacagcat tttgttttta cactgatttt
     8821 tcattttctc agcagagaaa taattgatag taaagattac gtttatatta tgattgataa
     8881 gactataggg tatatcttgc caaaaaaaat atttagttct ccttctgata tagatacatt
     8941 cctaaagcaa atcaatcaat ataaagaact gaagcaatag ggtcaggctt tgattatttg
     9001 gcttttatgg gttaatatgt taaatttatt gatctatggc acgaaaaccc agaatacatg
     9061 ttcctggtgg attttatcat gtaatactaa gaggtaatgc aggccaggac atattttatt
     9121 ctgtagaaga taccaaatat tttgaaagcc tgatatctga gggtattgaa aggtttggtc
     9181 acagagtgca tacatactgc tggatgaaaa accatgtgca tatggtaatc caggttgcca
     9241 atatcccact atcaaagatt attcagaata tttcatttcg ctatacactg tacatcaaca
     9301 aaaaatatga caagaccggc catctgtttc aaggcaggta caaggccatt ttgatcgatc
     9361 ctcaaagtta tttgttgcag cttgtaagat acattaattt aaacccgttg actgccaata
     9421 tagtaaataa acccgaggaa tataggtgga gcagttacag agcatttgtt ggtaagtgca
     9481 ggacggactg gcttactttg gactatatct taaagctatt ttcagacaat aaaagcgagg
     9541 ctattgagca atatcaggag tttgtacagg atcgtgatga agccgatttg aatatagatt
     9601 tttcatccgg gaataagcac ggttatgtgc tgggagatga tttgtttgca gagaaagtga
     9661 ttcaagcttc acaatctatt gataaaaacc gtatgagtat agaaaagata ataaccacgg
     9721 catgtaggtc gtttgacatt agtaaagatc agttgatttc caatgataga gcaagggacc
     9781 ttggaaggat tcgaactatt ataggttatc taataataga atatggaagc agcagtctct
     9841 ccgaatatgc aaggtatata aaacgggaca tttctacaat aagtggtgct gttgccagat
     9901 atcgtaaaaa actgttatct caaagtgaag agcaaatata tatggccaaa ctcgaaaaag
     9961 agctaggagt ctaaagttaa taaccaaaac accaaagcct gaccctattc catcgatgta
    10021 ttgttgtagg gggttgcgcc gaatacttca agtattactt taaataattg tcaaaaacct
    10081 ttgattttca atcggcaagc caatttaaat attcattcta acccccctaa aaaattgatt
    10141 attttttcat tttcggcttg acattaacgc cattttttta tatagtggta tcaagtggcg
    10201 taaagtggct tcaggtgtac caaaatgttt agaggacgat atgagcatac tatgactgat
    10261 aaggggaggg tgagtgttcc ttcacgcttc agggatcttc ttaagtctaa atacgaagac
    10321 gagaccctta ttattacaaa tttcaataaa tgtcttgctg cttatcctct acaggaatgg
    10381 tatgagattg agaataaagt tgcagagctt ccgcagttca aacaagaagt aatatccttt
    10441 ttaagatatt tgatgggtgg ggccgttgaa tgtccaatcg acagtcaggg aagaatactg
    10501 attcctcagt cactaagaag ttatgccagt cttgataaag atgttgttct tattggaatg
    10561 ttaaacaggt ttgaggtgtg gtccagggag gtttggggaa gtgaagagtc tgtaaaatct
    10621 tatgaagagt ttcaaaagag cagcgaaatt ctagccggtc agggtattta agatgctaaa
    10681 tacagcaatg aagatagaac aaaacaacat acatgtctca gttctgaagt ccgaaatcgg
    10741 tcagtatcta aggccatttg gtggtggatt atacattgat gctacccttg gttttggagg
    10801 gcacactctt gaaatactcg aaagagctgg ctataaagca aagttgatct gctttgaggt
    10861 tgataccgat gcaattgcaa taacatctga actcctctca tcttatagtg agaatgtaac
    10921 catagtaaac agaaatttta gtgaactcaa aagtgttctt gatcaatcag gaatagaaaa
    10981 agtagacgga attgtagcag acctgggttt atcttcctat cagcttgaac aaagcggcag
    11041 gggatttagt tttcaaaaag atgaagtatt ggatatgaga gcagatatgg attccgattt
    11101 aactgccttt gacataatta atgagtattc tgcccaagat cttgcagaaa tattttggga
    11161 atatggtgaa gagcgttttt caagacaaat tgcaaaggcg attgtacgta aaagagaaga
    11221 aggtgagata tctactactt tacagttatg cgatatcata gaaaaaacta tacctcagag
    11281 gtttaaacca aagaaaataa actgttctac aaaagtgttt caagcactaa gggtttgtgt
    11341 aaataatgag cttgataatt taagtttgtt catagaaaca gcaatagaaa ggcttaaagt
    11401 tggagctaga attgctataa tctcttttaa ttcacttgag gataaaatag taaagagagc
    11461 ttttgctaaa tacacgagcc cctgcacatg tccttctgat ctgccggttt gcagctgtgg
    11521 gaacaagcct gctatcaaac tcataaataa aagagctata agtcccggtg aagcagagat
    11581 cagatcaaat ccaagaagta gaagtgccag gttgagagta gcagaaaaaa tattatagaa
    11641 gatacaaaat gagagagaaa ctaaaacaga aagttagttc tagagaaatc ttaatcttga
    11701 tattatttat tgtcgggttt tttatgtttt atctgcatac aaaaatagaa ctaatagaag
    11761 taggttatga aatttctgac aataagaatt ataaaagatc actttcggaa aaaaatcaaa
    11821 tgctaaaagc ggagttttta aatctaaaat cacctgacag gattgaaaag cttgctttaa
    11881 aaagggggct tatatatccc tcgcaaaaag acatattgta ttcagggaac aacaaagatt
    11941 taagcgcata tagtggaaaa gatgaataac aataaataca aaagcaaatt aaatcttgca
    12001 agaaattccg ataagccaaa atggaagcta tcctgtatcg ctgtatttat ttgttttatg
    12061 tttgtacttg tagcagcaag gtctgcacag ctgcagctgc taagagatga taaagcttgt
    12121 gtagttggaa aagaccagca tgaatttgaa attgagcttc tgccagacag aggggcgatt
    12181 aaagatgcag ggggaaagga cctggcgatg agtgttactt cctattcaat ttatgccaat
    12241 cctctcaaga ttgaaaacaa agatcagttt gtatcaaaag tatctaaaac tattgatctt
    12301 aaaaaggatt atatagagaa aaaaataaat gcacaaaaat catttgtgtg gcttaagcgc
    12361 cttgcaagtg aagaggataa actctcacta cagcagctcc aactttctgg gcttggatat
    12421 attgaagaac ccaaaagagt ttatccttac aatcatctgg caggacaact tattgggttt
    12481 accaacattg attcgaaggg catagaggga ctggaatatt tatacgatac ttatctgtct
    12541 ggcaaacgag acaagctcaa gttaaaaaaa gacggacttg gaagggtgat atttgaacat
    12601 ccttcggaaa taaaagatga tgcccttggt tatgatattg ttcttacaat cgattctcat
    12661 atacagcata tagctgataa ttcacttaag aaacatgtgg agtcgtttga tgctgacagt
    12721 ggttttgcta ttgtgataga ctcagaaaaa gggtcagtgc ttgcaatatc gtcttatccg
    12781 tttttggatc ctaataagtt ttccgattcg agtgatcttg ccagaaaaaa tctgccagtc
    12841 tggaaatcat tcgagccagg atcaactcta aaacctttat tggttgcagg agcaattgaa
    12901 gaaaagctta ttaatcccaa cagcaaattc gattgtgaaa atggaaggat gaaagtaggt
    12961 gattcaataa ttaatgatgt tcatccttat aaaattcttg cagtatctga tgtgataagt
    13021 ctttctagta atatttgtgc ttctaaaata ggtgtaaagc ttgggaaaaa caggctttac
    13081 gactatttgc atgattttgg atttggcaaa aaaactgaaa tagaaatacc gggagagtct
    13141 tcaggactgc ttttaagttc agattcctgg gggcctgttg aactagcaac tatatcgttc
    13201 ggacagggaa tatcggtatc tgcactgcag ttagcaaatg cattttcatc aattgcaaat
    13261 aagggctatc ttatgaagcc ttatatagtt aaagaaatag tcgattccaa gggcaatata
    13321 ataatgagga atatcccaga aatcaggaaa agagtaattt catatgaaac agcgaacatg
    13381 gttgccgaca tgcttattga agctgtggac aatggaacag gaaaaaatgc acaggttaaa
    13441 cattataaag ttgccggcaa aacaggaaca gcgcaaatac cagaccctaa gaacggcggt
    13501 tactacgctg acaaatatat ttcgtcattt gcgggatttg ctccaattga tcccccgagc
    13561 ataacagttg ttgtagtggt tgaaaattct aaaaatggat tttatggggg tgaaatagca
    13621 gctcctattt ttaaagagat tgtagaaaag acactttatt atatggaaat aagtccggag
    13681 ggtaaggggc ttgataatat gattatgcct aacttcgagg gtaagagcag aagagaaata
    13741 ttaaggtggt ctgagacaga aggagtaaag gtgaatctgt ctggaaaggg ttttgctata
    13801 gatcaaagtc caaaaccagg agaaataata aattcaaaag aggtctgttc tgtaaaactg
    13861 gatcaaacta tatgaaccta agtgatttgc tgcagggcta tgaatgcaaa ttaataaatg
    13921 gagataaatt caaggttgta gagagtatag aatacagctc gagggaggtt aataagaatt
    13981 cactttttgt tgcaattaaa ggtgaaaagc tcgatggaca tagatatatt agtgacgcag
    14041 ttaatagggg ggcaggcgta atagttgttg aagattcggc gggggaaaaa caacctgagt
    14101 ctgtgacaat tgttgaggtc aaggattctc ttgatgcgct tgcctattta tcttcaaagt
    14161 attatggaga tcctacaaaa gatataagaa ttactgctgt cactggaaca aacggtaaaa
    14221 caacgattac ttatatacta gaatctattt gtacggggga aaatcaaata gtaggtgtaa
    14281 ttggaacaat aaattataga tataataaca ggcagtatcc ttccagcctg actacgccgc
    14341 tttcaaaaga gctgcagcat atgttttttg aaatgaagga gtccggggtc cagcatgtcg
    14401 tggttgaagc ctcatcacat gcgctggata agaagagact tgattattca aacattgact
    14461 gtgctgtttt tacaaacctc acccaggacc atctggatta tcataaggat attggttctt
    14521 atttcctggc aaagaagagg cttttttcaa atgtgctggc acattcttgt aaggataata
    14581 aaattgcagt ttcaaactac gatgatccct atggaaaaaa gatagtagaa gggtttgatg
    14641 gcaatatatt attttattct attgataacc ctgtagcaga catttatgca aaagatatac
    14701 agattagtac agatggaata aaggcaaggg cagtaacacc ggctggagag gtagaaataa
    14761 attcggagct tcttggaagg cataatttat caaatatttt agcctcagta gcttgtgctg
    14821 tcggtgcggg agtctcggcg gatgatattg caaaaggtat agcaactctt aaaaatgttc
    14881 cgggcaggct gcaaagagta agtaattcta aggggataaa tatatttgtc gattacgccc
    14941 acactcccga tgctcttgga aatgtactgg tttcagtaaa aagtttatgc cggggcaggc
    15001 ttatagttgt ttttggttgt tgaggtgacc gggataaaga aaaaagacct atgatgggaa
    15061 c
//
LOCUS       DBNGSV010000022         6680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1318854, whole genome shotgun sequence.
ACCESSION   DBNGSV010000022 DBNGSV010000000
VERSION     DBNGSV010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1318854"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..462
                     /locus_tag="AC159U_01015"
     CDS             76..462
                     /locus_tag="AC159U_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3335669.1"
                     /translation="MLVKECMTKDPITISTDEDVQYAFYILKKHDIKQLPVTNNGYIL
                     GIVTERDLRMLIEKEYGIKIESAMSTNLQTISEDDRIEKAAQIIHYKKYNALPVVDNT
                     DKLVGIITVTDIIEGLLTIINDQKNQ"
     gene            518..964
                     /locus_tag="AC159U_01020"
     CDS             518..964
                     /locus_tag="AC159U_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3335670.1"
                     /translation="MFYLKGFNYIITIILYVLIANFALSQYEEDPQELNEEQIIENIS
                     PEQAYKMIEANTGNNNFIILDLRTPQEHREEHIEKSKNINFLSENFRKELNNLDKENT
                     YIIHCRSGSRAAKTLPIMKESGFKKVYNFQGIIQWKEAGLETVKTK"
     gene            980..2050
                     /locus_tag="AC159U_01025"
     CDS             980..2050
                     /locus_tag="AC159U_01025"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3335671.1"
                     /translation="MLKLLRYSLLAFILLTIPEALAQDDLQTKAQALFKAIPDSPPQF
                     ENNPATADKIKLGKMLYFEPRLSASHLISCNTCHNLGTGGVDLQETSVGHGWQKGPRN
                     APTVLNAVFNIAQFWDGRAKDLADQAKGPVQATVEMNNNPQRVVATLKSMPEYRNLFA
                     LAFPKQKEHTSFENVVKAIEVFEATLLTPDSRFDEFLKGNAKALSSQEKEGLRLFMDK
                     GCASCHNGINLGGNSYFPFGVVQNPGAEILPPGDKGRFKVTKTASDEYVFKVPTLRNI
                     QLTPPYFHSGKVWSLKEAVSIMGSSQLGIKLDDNQIGKITIFLNSLNGKQVKTEYPIL
                     PANTEDTPKPILKTINGSNQGH"
     gene            complement(2127..3755)
                     /locus_tag="AC159U_01030"
     CDS             complement(2127..3755)
                     /locus_tag="AC159U_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013326478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR3335672.1"
                     /translation="MSNIQIIIQTAGGIGLFLLGMIVLTDGLRTLAGSAMRSVLMRFT
                     SSPATGALTGAASTAVLQSSSATTVAAVGFVGAEILTFPQALGIIFGANIGTTFKGWL
                     IAILGFKLSLVNIFLPLVFIGAVLRLFTKGRLSAIGYSIAGFGLIFVGITAMQQSMIE
                     LHGFISFQNLPADTFLSRILLLGIGLLFSAITQSSSAGVVAALTALFTDLINFNQAAA
                     LVIGMDIGTTITAAMATIGGSVGAKRTGFSHVIYNFLTATMALFLITPYTSIWEYLAP
                     GQLINNAEIALVAFHTFFNTLGVFLIIPFTGHFAGFMEKLIPGKISTYTQKLDFTLLE
                     DPNLALNAVQTTIKSETLGLLSHISAILGGKGKEKRIDIDELQSAINDTQSYIDKINV
                     ESGMGVNWERLVNMIHALDHLQRLLERCEEQEDRAITARESADLSVQADLLKISIDKI
                     LGDIKNHHWTHAVWQAKDTSSKIHKLVKPFRQSVMDKVASGQYDVATGTENLEAIRWL
                     RRVSKHIARIIQHYEQSILASGKTSQGNKANPGI"
     gene            complement(3850..4515)
                     /locus_tag="AC159U_01035"
     CDS             complement(3850..4515)
                     /locus_tag="AC159U_01035"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3335673.1"
                     /translation="METNADYNYTNARTNMVNEQIKARGIKSKKLLTTFLRVPRHLFI
                     PKEYSLYSYSDGPLPIGEDQTISQPYIVALMTDLLDLQKGDKVLEIGTGCGYQTAILS
                     DLGCRVYTIEIIESLGLRAKETLDNLGYENINFKIGDGYQGWKEKSPFDAIIVTACPK
                     VLPKPLVDQLKIGGRMVIPLEEEYQELKLIIKEKSGLTKKRITLVRFVPMTGEAENAS
                     YNV"
     gene            complement(4701..5168)
                     /locus_tag="AC159U_01040"
     CDS             complement(4701..5168)
                     /locus_tag="AC159U_01040"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3335674.1"
                     /translation="MPVQKLKNYLDENNIKYITIKHSPAFTAQQIAENAHVSGKELAK
                     TVMIKLDGKMAMAVLPASYHVDFKLLEEASGAIRISLADEQEFKDMFADCEVGAMPPF
                     GNLYGMDVYVARSLAEDEEIFFNAGTHSELIRLSYKDFERLVKPKILKFSEKN"
     gene            complement(5622..6530)
                     /locus_tag="AC159U_01045"
     CDS             complement(5622..6530)
                     /locus_tag="AC159U_01045"
                     /inference="COORDINATES: protein motif:HMM:NF039394.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HIR3335675.1"
                     /translation="MNKYLYILITICFIYIPDSFSQVHKQSELNVGITTNYDHGIDTD
                     IDNGGEISINRYSISVNGSRHINDRVGLDINTSYSFTDFDFSANSGFAEPNPWEGINK
                     AGLGFRLKYSINPEWSLSAGPFIRYSGEQGADFSDSLTYGGLAGFTYSPSRNFFIGTG
                     VYLSSRLEDHVIAYPGLIMNWVVSEKIRVSTLLTGVKSELGPRVQIYYEITDKIDTAI
                     SAGYEFNRFRLDDESIAQDGIGDVKILPVWASIGYEVSKHIRLELYSGFGFLGEIELE
                     DRNGDRLEKEDFDTMFFIGLGFKINK"
BASE COUNT         2165 a         1344 c         1131 g         2040 t
ORIGIN      
        1 tggaatatcg gttttatcag agcaattaag cgataagaat aatgtaataa aattaaatta
       61 tggaggtgat accttatgct tgtaaaagaa tgtatgacaa aagatcctat tactatatca
      121 accgatgaag atgttcagta tgcgttttat atattaaaga aacatgacat taaacaacta
      181 ccagtcacaa acaacggata tatattaggg attgtgactg aaagagatct gagaatgtta
      241 attgaaaaag agtacggtat aaaaatagaa tctgctatga gtactaatct gcaaacaata
      301 agtgaagacg ataggattga gaaagctgct caaatcatac attacaaaaa gtataatgct
      361 cttccagttg tggacaatac ggataaactt gtaggaatta taaccgtaac agacattatt
      421 gaaggacttt taacaattat aaatgatcaa aagaatcaat aatactaaac tttgtcagta
      481 tatataaatt tgtatattgg ggaacataac gataaccatg ttttatttga agggatttaa
      541 ctacataatc acgataatcc tttatgtgct tatagcaaac tttgcgctgt ctcaatatga
      601 agaagaccca caagaattaa acgaagaaca aatcattgaa aacatatcac ctgagcaagc
      661 atacaaaatg attgaggcta atacaggtaa caacaatttt attattcttg atctaagaac
      721 accccaagag catagagaag aacatattga aaaatctaaa aacattaact tcctttcaga
      781 aaattttaga aaagaattaa acaatctgga taaagaaaat acctatatca tccattgccg
      841 atccggtagt agagcagcaa aaactcttcc aattatgaaa gagtctggtt ttaaaaaagt
      901 atataatttt caaggaataa ttcagtggaa agaagccgga cttgaaacag tgaagaccaa
      961 ataaaatcgg gataaatata tgcttaaatt actgagatat tcattattag catttattct
     1021 acttactatt ccagaggctt tagcacagga tgatttacaa accaaagccc aggccctgtt
     1081 taaggcaata cctgatagcc cgcctcaatt tgaaaacaac ccggcaacgg ctgataaaat
     1141 caagcttggc aaaatgctgt attttgaacc tcgcttgtct gcctctcatt taataagctg
     1201 caatacttgt cataacttag gcacaggtgg tgttgacctg caggaaacct cagtaggtca
     1261 tggctggcag aaaggtccta gaaacgcgcc tacagttcta aatgccgttt tcaatatagc
     1321 acagttttgg gacggaaggg caaaggatct cgccgatcag gctaagggac ctgttcaggc
     1381 aacagtagag atgaataata atcctcaacg agtagttgca acgctaaaga gtatgcctga
     1441 atatcgcaat ttatttgctc tggcttttcc aaaacaaaaa gaacacacta gctttgaaaa
     1501 tgttgtaaag gctatagagg tatttgaggc aacacttcta acacctgatt cgaggtttga
     1561 tgaatttctt aaaggaaacg ctaaagctct aagcagccag gaaaaggaag gattaaggct
     1621 cttcatggat aagggatgtg caagttgtca caatggaata aaccttggcg gaaatagcta
     1681 ctttcctttt ggtgtagtac aaaatcccgg agctgaaata cttccccctg gagataaggg
     1741 ccggtttaag gtaacaaaga ccgcaagtga tgaatatgtg tttaaggtcc cgaccctgcg
     1801 taatattcag ctaacacctc catactttca ttccggcaaa gtatggagcc tgaaagaagc
     1861 tgtatccatc atgggctcat ctcaattagg aataaaactt gatgacaacc aaatcggtaa
     1921 aattaccatt ttcctaaact ccctaaatgg caaacaggtt aagacggaat atccaatact
     1981 acctgccaat actgaggata caccaaaacc tatattaaaa actataaatg ggagcaatca
     2041 aggtcattag taatatcttt tatactattt aaacgctagc tgaaaataat cagtagaaca
     2101 actacagttg ccattattaa catgattcat atgcccggat tggctttatt tccttgagat
     2161 gtcttaccgg atgccagaat agattgctca taatgttgta ttatccttgc aatatgttta
     2221 ctaacacgcc ttaaccatct aatagcctca aggttttctg tgcctgtcgc aacatcatac
     2281 tgtcccgatg ccactttatc cataacgctc tgtctgaagg gttttaccag cttgtgtatt
     2341 ttcgacgaag tatctttagc ctgccaaacg gcatgtgtcc aatgatgatt tttaatatct
     2401 cctaaaattt tatcaatact gattttaagc aaatcggcct gcactgaaag atcggcactc
     2461 tctctagcag ttattgccct atcctcctgc tcttcgcatc tctcaaggag ccgctgcaaa
     2521 tgatccaggg catgaatcat gtttaccagc ctttcccagt ttacccccat cccggattca
     2581 acattaattt tgtctatata agactgtgta tcgttaattg ccgattgtag ttcatcaatg
     2641 tctattcttt tttctttgcc cttaccgcct agaattgcgc taatatgtga gagtaaaccc
     2701 agggtttcac tttttatcgt agtttgaact gcattaagtg ctaaatttgg atcctcaagc
     2761 aaagtaaaat caagtttttg agtatatgtt gaaattttac caggaatcaa tttttccata
     2821 aaaccggcaa aatgccctgt aaacgggatt atcagaaaca cgcccaatgt attaaaaaat
     2881 gtatgaaacg ctacgagcgc aatttctgca ttatttataa gctgcccggg ggcaaggtat
     2941 tcccatattg aagtgtatgg agtaattaag aacaaagcca ttgtagctgt taggaagttg
     3001 taaatcacat gggaaaatcc tgttcttttt gcaccaacag aacctccgat ggtagccatt
     3061 gctgccgtaa tcgttgttcc tatatccatt cctattacta aagcagcggc ttggttaaag
     3121 tttatcaggt cagtaaagag tgcagtcaga gccgcaacga ctccagcact tgatgactgg
     3181 gtaatcgccg aaaataaaag cccaatacca agaagtaata ttctgcttaa aaaagtgtct
     3241 gcgggaaggt tttgaaatga aataaaaccg tgcagttcta tcattgactg ctgcatagcc
     3301 gtaattccaa caaatatcag tccaaaaccg gcaatcgaat acccaattgc actaagacgt
     3361 cccttggtaa aaagtctcag cactgcacct ataaatacca gcggtaaaaa tatatttacc
     3421 agacttagtt taaatccaag gattgcaatc agccagccct taaaagtggt cccgatatta
     3481 gcgccaaaaa taatcccgag cgcctgggga aatgttaata tctctgcccc cacaaaaccc
     3541 actgctgcca cagtagttgc gcttgatgat tgcagaaccg ctgtacttgc ggcacctgta
     3601 agagcacccg ttgccggact gcttgtaaaa cgcataagta ctgaccgcat tgcgcttcct
     3661 gcaagcgttc gaagtccgtc ggtaagcaca atcatcccta gcaaaaaaag accaatgcct
     3721 ccggcagtct gaattattat ttgaatattg ctcattagcc ctgccagtta ttaaattaat
     3781 atataaatgg caatcttgat tatagttgtt gatatttagc ttatactgag aggaaatatc
     3841 atataaattt cacacattat aactagcatt ttctgcttca ccggtcatag gaacgaacct
     3901 caccagggtt attctttttt ttgtcagtcc acttttttcc ttgataatta attttaattc
     3961 ctgatattcc tcttctaacg gaattaccat tcgcccccca atttttaact gatccacgag
     4021 gggttttggc aatactttag gacaagcagt tactattatt gcatcaaaag gagatttttc
     4081 tttccacccc tgatagccat ctccaatttt aaaatttata ttctcatagc caagattatc
     4141 cagggtctcc tttgctctaa gacccaggct ctcaataatt tcaatagtat ataccctgca
     4201 gcccagatca gagagaatgg ccgtttgata accgcaccct gtacctatct caagaacttt
     4261 atctcctttt tgtaaatcta ataaatcagt cattaaggca actatatatg gttgggatat
     4321 agtttgatct tctcctattg gcaaggggcc atcagaatag gaatacagtg agtattcttt
     4381 gggaataaac aggtggcgcg ggactcttaa aaatgttgtt aataactttt tgctttttat
     4441 gccgcgggcc ttaatttgct cgttaaccat attcgtacgc gcattcgtgt agttgtaatc
     4501 tgcattagtt tccattgtaa taccctcata ctattgccct gatctcataa accagattgc
     4561 tgcaaatatt attaaagcga ctactatgat ataaacaaac cctccgccta ttgcttccat
     4621 attaccttcc cataaaaatt atgtatttaa ccgacttttt tcttttgatt aattcccccg
     4681 tactgaatta tataattatt tcagtttttt tccgaaaatt tcaatatctt tggttttacc
     4741 agtctctcaa agtctttata agaaagcctt attaactcgg aatgagtacc ggcgttaaag
     4801 aatatctctt catcttcggc aagactccgg gctacataaa catccatgcc gtagaggttt
     4861 ccaaacggcg gcatggcacc gacctcacaa tcggcaaaca tatctttaaa ctcctgctca
     4921 tccgcaagac ttattctaat tgctcccgat gcttcctcaa gaagtttaaa atctacatgg
     4981 taagaagcag gaagcaccgc catagccatc ttaccatcaa gtttaatcat aacagttttt
     5041 gcaagctctt ttccagaaac atgcgcattc tccgctatct gctgagcagt aaatgcggga
     5101 gagtgcttta tggtaatata ttttatatta ttctcatcta aatagttttt aagtttttgt
     5161 acaggcattg tttaccccct tatttaaatt actcaaaact agattgttgt tgtttagttc
     5221 ctactactag taattcaatt aaggtgccaa aataatattc aatagttaaa ggtaattaga
     5281 tttatatata tttggtatgg tgtctaataa tcttacagct tacatcacaa gatatttaaa
     5341 gtggttaatt aatagaattt gatataattt aataattgag gaaatttgat aaatactgcg
     5401 ataataaatt aagatgtcag tgtctatgtc ggtaagtcaa ttggctattt catacaaaca
     5461 gactaaacaa aaaatgcagg aaaggaattt tttgcacaaa tactgaattt attgaatgtt
     5521 ttgcaatgtc ggaaaatcca ggaacatccc gttttagcct ttaggacgca atggcctctg
     5581 gtcagagctc ggtcacattt atggataatt ttaaaaccaa tctatttatt aatcttaaaa
     5641 cccaaaccta taaaaaacat agtatcaaaa tcttcttttt ctaatctatc tccgtttcta
     5701 tcttcaagct ctatttcacc caaaaaccca aaccctgagt atagctctag tcttatatgt
     5761 ttacttactt cataaccaat gcttgcccat acgggaagga tttttacatc tccgattcca
     5821 tcttgagcta tactttcatc atccaaccta aaacgattaa attcatagcc tgcgcttata
     5881 gcagtatcaa ttttatcggt tatttcatag taaatttgaa cccttgggcc aagctcactt
     5941 ttaacccctg taagtagagt agataccctt attttttcac tgaccaccca gttcataatt
     6001 aggcccggat aagctatgac atgatcctcc agtctgctgc ttagataaac tcctgtacct
     6061 atgaagaaat ttctgcttgg cgaataagta aaaccggcaa gacctccgta ggtcagagaa
     6121 tcgctaaaat cagctccctg ctcaccggaa tatctgataa acggtcctgc ggataatgac
     6181 cactccgggt ttatgctgta tttaagtctg aatccaagac cggctttgtt tattccctcc
     6241 catgggttcg gctctgcaaa cccactgttt gcagaaaaat caaaatctgt aaagctgtag
     6301 cttgtgttta tgtccaatcc aaccctatca ttaatatgcc tagaaccatt tacactaatt
     6361 gaatatctgt taatgctaat ctctccacca ttatcaatat ctgtatcaat cccatgatca
     6421 taattagtag ttatacctac gttaagttca ctttgcttat gaacctgaga aaaagaatca
     6481 ggtatataaa taaaacaaat ggttattaat atatataaat atttattcaa aatgctcccc
     6541 ttgtaaatta attattaagg agctcgcata gaacagagct ataagtaatg cagaatctcc
     6601 tttattttat taactaaatc tacatttctg ttattaaatc taaattccat ctcttttaga
     6661 tacaaaacaa agtgttttct
//
LOCUS       DBNGSV010000023         3167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1665313, whole genome shotgun sequence.
ACCESSION   DBNGSV010000023 DBNGSV010000000
VERSION     DBNGSV010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1665313"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC159U_01050"
     CDS             complement(<1..189)
                     /locus_tag="AC159U_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335676.1"
                     /translation="MRLILNKTFVAAVLHSLSVFLISMCTGISGVYAVTKLDGCGDLN
                     TKGETYNLINDIVHKGPGD"
     gene            442..2505
                     /locus_tag="AC159U_01055"
     CDS             442..2505
                     /locus_tag="AC159U_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3335677.1"
                     /translation="MIRAYSKIILCLLLFMGCILASSVSKALENGSFNINKFGSYNKT
                     DSKINPLITKTLKARDRFITLQNETLKANQVSTPFVKINDSGAVQVYLYLTGINNEVL
                     SLLKDINFEVEIINKDLNMIQGWLSEDSLNILSENEHVLKISPPSYGFTKQGTVKTEG
                     DEVLNSDDVRDMLGIDGAGVIVGVISDGVDSLIASKVSGNLPDVVTVLKPGIGDEGTA
                     MLETIHDIAPGAELCFSDGFSSSMAFMKSVDDLINICDVNIIVDDVGFLLEPYFQNGT
                     VAQKVDEAVSNGVIFVTAAGNNADEHYQHLFVQEVDDDELNLHDFGFASGGPSEISIP
                     ILVGGTDFAPNNFIAAVLQWSDPFGGSSNDYDLFLFDDEGVQIDSSTAIQDGSQDPVE
                     VVLFTNNSPNIVMVDLVINRFSGVPRVLELQFNGVITLLDFNVPTDSIFGHSGAQGAV
                     SVGAVPHFDPNTIEDFSSRGPVSIHFGPLNELNSINSSSGFTEISNNTPIETRMKPDV
                     VAPDGTSTSLSGFSPFFGTSVSAPHVAGVLALMIDANPQLEQISANTELNSKFNEKNI
                     INNPNAFINILKNTAVDLGAPGPENTFGSGRVDAFAAVQQVLSQSPSPTPTTTPIPTP
                     TPTTQPSPTPTTTPSGSGDTTSSGCSVTDANAESGIFNSFLTILVLLTALAINKNLRN
                     RRRDI"
     gene            complement(2502..>3167)
                     /locus_tag="AC159U_01060"
     CDS             complement(2502..>3167)
                     /locus_tag="AC159U_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIR3335678.1"
                     /translation="RIHDSLKPDSMEELKDKLKGLGYNKKIKNKVKASEVQEILNFFR
                     DTPKEYAANLFILRSMSRAIYGTKDTGHFGLALDYYTHFTSPIRRYPDLIIHRLLDSL
                     IDKKEPIYDQKMLSTISSHCSEKETVSDEIERESMKLERAFVLQRYMGRTMKGIIISI
                     QSFGLFVELIDIYAEGFVPRKKIRGFDESRFSIGQNVTVKISECDIDNRRAAFDLIKI
                     HKH"
BASE COUNT          957 a          654 c          615 g          941 t
ORIGIN      
        1 gtcacccggc cctttatgaa caatatcgtt tataagatta tatgtctcac cttttgtatt
       61 tagatcgcca cagccgtcaa gttttgtaac agcataaaca ccgctaatac ccgtacacat
      121 ggaaatcaaa aagacggata acgaatgcaa cactgctgca acaaacgttt tgtttaatat
      181 taatctcacc accactatat tatttatatc taatacccag gtagtagttt aatcacatat
      241 ttttttaaag taaacttaga tataaaaaca gatatgaata ctctttagtc ctctattctt
      301 cttttgttcc tactcctaat tatcatcatt taatgcatct attacatttc atatacattt
      361 actattgaga acaccacttt tatactctat ctttatagat caatatatat aagttaataa
      421 tgcagtttta gggagggtcg agtgattaga gcatacagta agattatatt gtgtttactt
      481 ctgtttatgg gttgtattct tgcttcatca gtatcaaagg cgcttgaaaa tggtagtttt
      541 aatattaata aatttggaag ctataataaa acagactcaa agataaaccc tcttatcacc
      601 aaaaccctga aggcccggga cagatttata acactgcaaa atgaaaccct taaagcaaac
      661 caggtttcaa ctccgtttgt aaaaataaat gattcaggcg ctgtacaggt ctatttatat
      721 ttaacaggga taaacaatga agttctttca cttcttaaag acatcaattt cgaggtagaa
      781 ataataaaca aagacttaaa catgatacag ggctggttat cggaagatag tctaaacata
      841 ctatccgaaa atgaacatgt gttaaagata tccccgccga gctacgggtt taccaaacag
      901 ggcacagtaa aaacagaggg ggatgaagta ctcaattctg acgacgtaag ggatatgctt
      961 ggtatagatg gcgctggagt tatagttggg gtcatatcgg acggtgtcga ctctcttata
     1021 gcttcaaagg tatcaggaaa tcttccagat gtggtaacag ttttaaaacc aggaataggt
     1081 gacgaaggaa cggcaatgct agagaccata cacgatattg cccccggggc cgagctttgt
     1141 ttttccgatg ggttttcttc gagcatggca tttatgaaat ccgtagatga tcttattaat
     1201 atctgtgacg tcaatatcat tgtagatgat gtcgggtttc ttttagagcc ctattttcag
     1261 aacggcacag ttgcccagaa agtagatgag gctgtgtcaa acggagtaat attcgtaaca
     1321 gctgctggaa ataatgccga tgagcactat cagcatctat tcgtacaaga agtggacgac
     1381 gatgagctca atctgcatga ctttgggttt gcatccggcg gtccgagcga aatttcaatc
     1441 cccattttag taggaggcac tgattttgcg ccaaacaatt ttattgctgc agtactacag
     1501 tggtctgatc catttggcgg ctcatcaaat gattacgacc ttttcctctt cgatgatgaa
     1561 ggtgtacaga ttgacagcag caccgccatt caagacggct ctcaggatcc cgtagaagta
     1621 gtacttttta caaataactc acctaatata gttatggttg atcttgtgat aaacagattt
     1681 agcggagtgc cgagggtgct tgagcttcag tttaacggag ttatcacgct cttggacttt
     1741 aatgtaccca ctgacagtat cttcggtcat tcgggagcgc aaggtgcagt ttctgtagga
     1801 gcagtaccgc attttgaccc taatactatc gaagatttca gctctcgggg tccggtttca
     1861 atacattttg gaccgttaaa tgaactgaac agtattaaca gctcatcagg ttttactgaa
     1921 atatcaaata atactcctat agaaacaagg atgaagcccg atgtggtggc cccagacggc
     1981 acaagtacat ctttatcagg cttttctcca ttctttggaa catccgtatc tgctcctcat
     2041 gtagcagggg tactggcgct tatgatagat gcaaatcctc agcttgagca aatttcggca
     2101 aataccgaat taaactctaa atttaacgaa aaaaacatta tcaataatcc aaatgcattt
     2161 ataaacattt taaaaaatac agcagttgat ctcggagcac cggggcctga aaatacattt
     2221 ggttcaggaa gagtagacgc ttttgcagca gtacagcagg tgctaagtca gtcaccaagc
     2281 ccaacaccca cgacaacccc aattcccact cccacgccta ctacacaacc ctcacccaca
     2341 cctacgacca cgccatcggg ttcaggagac actaccagca gcggctgctc ggttacagat
     2401 gcaaatgctg aatcaggaat attcaatagc ttcttaacta ttttggtgtt gctgacagca
     2461 ttggctatta ataaaaattt aaggaataga agaagagata tttagtgttt gtgaatcttt
     2521 ataagatcaa acgctgccct tctgttatca atatcgcatt ctgatatttt aacagtaaca
     2581 ttctgaccga ttgaaaatct ggattcgtca aagcccctta ttttctttct gggaacaaac
     2641 ccctcggcat atatatcaat cagctccacg aacaggccaa atgactgaat gcttattatt
     2701 atccccttca ttgttcggcc catataacgc tgcagcacaa aagccctttc gagtttcatg
     2761 gactcacgtt caatttcatc tgatactgtc tccttttcac tgcagtggct cgagattgta
     2821 ctgagcattt tctgatcata aatgggctct tttttatcaa tgagactgtc gagcagcctg
     2881 tgtattataa gatcaggata acgccttatg ggagaggtga aatgagtgta ataatcaagc
     2941 gcaaggccaa aatgcccggt gtcctttgta ccgtaaattg ccctgctcat cgagcggaga
     3001 ataaacagat tggctgcata ctcctttggg gtatctctga agaaattgag gatttcctga
     3061 acctctgaag ccttaacctt atttttaatc tttttattat atccaagacc cttaagttta
     3121 tccttaagct cctccatgga gtctggtttt agggaatcat ggattct
//
LOCUS       DBNGSV010000024         4098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_280062, whole genome shotgun sequence.
ACCESSION   DBNGSV010000024 DBNGSV010000000
VERSION     DBNGSV010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_280062"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /gene="ftsA"
                     /locus_tag="AC159U_01065"
     CDS             complement(<1..1069)
                     /gene="ftsA"
                     /locus_tag="AC159U_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3335679.1"
                     /translation="MSRDANLIVALDIGTTKVCAIVAEIKSPEQVDVLGVGIHPSDGL
                     KKGVVVNIEKTVNSIKSAVEEAEEMSGCDIRNVYVGVAGGHVQGMSGHGMITLKSKEV
                     ALPDVDRAIDSASAVMIPSDREVLHVIPQEYIVDGQAGIKDPVGIYGMKLETKVHIVT
                     GQITAAQNLVKCVHNAGMDVSDMVLEQIASSEAVLSRDEKEIGVVLVDCGGGTCDVAI
                     FYGGTIRYTENITLGGDHVDRDISLGLSTPLSEARKIKEEYGSAYAELVPSDEVIQIE
                     SVGGRPPRKILRKDLAMIIEPRMEEIFSMVRKAINRSGYKDLIPAGAVITGGTINIEG
                     SQEIAEKVLGMPVRIGVPNSVG"
     gene            complement(1092..1814)
                     /locus_tag="AC159U_01070"
     CDS             complement(1092..1814)
                     /locus_tag="AC159U_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3335680.1"
                     /translation="MMLNERLINYLYVMLAGSILLTSILFVFTLNLYGYLTVQTINIE
                     GNYRVSQTEIMKRTNLEKGQSSLLFLDSDLEAKIEKSPWVLDATVTKILPNSVSIRIK
                     EAEIFCLIDSGNKNLKYISDSGIILDNANIEFGLDFPVIIGESIIEPELLEYALSILK
                     ISRYDPVLNFEHISELNVNSVYGITVVTTEGVNIFFGFGENLEERWNTLKKTFLFSQN
                     SNIKQQFIDLSLKDKVVVKYDL"
     gene            complement(1816..2700)
                     /gene="murB"
                     /locus_tag="AC159U_01075"
     CDS             complement(1816..2700)
                     /gene="murB"
                     /locus_tag="AC159U_01075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3335681.1"
                     /translation="MIESKLDKIGIEYIADYPMSRFTWLKVGGSANFVIYPKTSEQLA
                     VVINLLTKEGYPWVVLGEGSNTIILDKGIEKAVIITKKMKSISITDSGEVVAEAGANM
                     GTIMNQTVRKALTGFEFAAGIPGTLGGGVFMNAGANDAEIKDVIKKVWLLIDGKEEIV
                     NRSGLNFEYRKSNLPENAVVMKAEIKLEKGDRQHSEKQVKEYLEKRSRTQPIRMSNTG
                     SIFKNPENIAAGRLIEELGLKGYEIGGAQISQLHGNFIVNTGSAKASEVLELIDLAKE
                     NALKIRGIKLEPEVRIIG"
     gene            complement(2697..4076)
                     /gene="murC"
                     /locus_tag="AC159U_01080"
     CDS             complement(2697..4076)
                     /gene="murC"
                     /locus_tag="AC159U_01080"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3335682.1"
                     /translation="MYGRIKKIHFVGIGGIGMSGIAEVMLNMGYTVTGSDIRTSKVIE
                     RLEDLGAQVSIGHKAKNVRGRDVVVYSSIITDKNPEIIEANKLGTPVIPRAEMLSELM
                     RLKYGIAVAGSHGKTTTTSLISSVLDFGGFDPTVVVGGRIQSLGTNAYLGKGSFMVVE
                     ADESDGSFLKLSPVIAVITNIDDEHMDHYKDSQQLDDAFSDFINKIPFYGLAVVCGDN
                     KRAMKLLKSYNKRYVTYGLGKDSCYQARDVEFSYLKTSYNLYRGNKLEGLVHIQLPGI
                     HNALNSLVAFAIGYELGMSFDDINKGLEKFGGIERRIEVKGIVNGITVVDDYGHHPKE
                     IDVTIKAVLNSFSPRKLKVIFQPHRYSRTKNLFDEFVGVFKNISPLYLADVYAAGEKE
                     MKNVNSKLLAEAIKKAGNKDVHHYSDNNKLIEKIIRTAKPGDVVITLGAGNIWAVGEK
                     IVQELKKTS"
BASE COUNT         1091 a          975 c          617 g         1415 t
ORIGIN      
        1 ccccgacact gttgggtact ccgattctta caggcatacc aagcactttt tctgcaatct
       61 cctggctccc ttcaatattg atagttccac ccgtaataac agcacctgcc gggatgaggt
      121 ctttatagcc tgacctgttt atcgcctttc ttaccatgct gaagatttct tccattctcg
      181 gctctataat catggcaaga tcttttctaa ggatttttct gggaggtctg ccccctacgc
      241 tttcaatttg aatcacttca tcactcggca ccaactcagc atatgccgag ccatattctt
      301 cttttatctt ccttgcttca gacagtggtg ttgacaggcc aagcgagatg tccctgtcca
      361 cgtggtctcc gccaagagta atattttcgg tataccttat tgttccgccg tagaatattg
      421 caacatcaca agttcctcct ccacagtcca caagaactac tccaatttcc ttttcatcac
      481 ggctaagcac ggcctcactt gaagcaatct gctcaagaac catgtcggat acatccattc
      541 ctgcgttatg gacacattta acaagatttt gcgctgctgt tatctgacct gtaacaatat
      601 gaaccttggt ttcgagtttc attccgtata ttccaacagg gtcttttatc cccgcttgtc
      661 cgtctacgat atattcctgg ggaataacat gaagtacttc tctgtccgat ggtatcatta
      721 ctgcacttgc tgaatcaatt gccctgtcca catcgggcag tgcaacttct ttacttttta
      781 aggtaatcat cccgtggccg ctcataccct gtacatgacc gcctgctact cctacatata
      841 catttctaat atcacaaccc gacatttctt cagcttcttc cacagcactt ttaattgagt
      901 tcactgtttt ttcaatattt acaactacac ctttttttaa tccatcagac ggatgtatac
      961 caacaccaag aacatccacc tgctcaggtg acttgatctc agcaacaatc gcacaaactt
     1021 ttgttgtgcc tatatcaaga gcaacaatca aatttgcatc tctgctcatt atctttacct
     1081 ataaaattcc cttatagatc atatttaact acgaccttat cttttaggct taagtcaata
     1141 aactgctgct ttatgttaga gttctgagaa aaaaggaaag ttttcttaag tgtgttccac
     1201 ctttcctcca gattttctcc aaatccaaag aatatgttaa ctccctctgt ggttactact
     1261 gtgataccat atacagaatt tacattcagc tcagatatgt gttcaaaatt aagaactgga
     1321 tcataccttg atatttttag tatcgagaga gcatattcca gtaattccgg ttctattata
     1381 ctttcaccta taattacagg aaaatcaagt ccaaattcaa tatttgcgtt atcgagaata
     1441 ataccggaat cactaatgta ttttaagttt ttattgcccg aatcaataag acaaaatatt
     1501 tcagcttctt ttatgcgtat ggaaaccgaa tttggcaaaa tctttgtaac agttgcatct
     1561 aaaacccagg ggcttttttc tatttttgct tccagatcgc tatcaagaaa aagcagtgag
     1621 ctctgtcctt tttcaaggtt agtgcgtttc attatctcgg tttgtgatac cctgtaattc
     1681 ccctctatgt taatggtctg aaccgtaaga tatccgtaga gatttagagt aaatacaaag
     1741 agtatgcttg tgagcagtat tgagcctgca agcattacat ataaatagtt tataagtctc
     1801 tcatttaaca tcattttatc ctattattct tacctctggt tctaatttta tacctcttat
     1861 tttgagtgca ttttcttttg ccagatcaat gagttccaac acttccgagg cctttgctga
     1921 gcctgtattt acgataaaat tgccatgaag ctgcgatatt tgtgctcccc ctatttcata
     1981 acccttgagc ccaagctcct ctattaacct tcctgccgct atattttcag ggtttttaaa
     2041 tatagaccct gtgtttgaca tccttatagg ctgtgttctg cttctttttt caagatactc
     2101 ttttacctgt ttttcgctat gctgccggtc acctttttct aatttaatct ctgctttcat
     2161 aacaactgca ttttctggta aattactctt tctgtattca aaattaagcc cacttctatt
     2221 tactatttct tccttaccgt ctattaaaag ccatactttt ttaattacat cttttatctc
     2281 cgcatcatta gctccggcat tcataaagac tcccccaccc aatgtgcccg gaattcccgc
     2341 tgcaaattca aaacctgtaa gcgctttcct gactgtttga ttcattatgg ttcccatatt
     2401 tgcaccagcc tctgcgacca cttctccaga atctgtaata gaaatagatt tcattttttt
     2461 tgttatgatc acagcctttt caataccttt atcaagtatt atagtgttag agccctctcc
     2521 gagaacaacc cacgggtaac cctctttagt cagcaggttg ataaccactg caagctgctc
     2581 agaagttttt ggatagataa caaaatttgc actcccccct accttaagcc acgtaaacct
     2641 gctcatggga tagtctgcaa tgtattctat tcctatttta tcaagctttg attcaatcaa
     2701 cttgtttttt taagctcctg tactattttt tcgccgactg cccagatatt tcctgcgcca
     2761 agtgttatca ctacatcacc cggctttgca gtacgtataa ttttctcaat aagtttattg
     2821 ttatcactgt agtgatgcac atctttattt cctgcttttt ttatagcctc ggcaagcagt
     2881 tttgaattca catttttcat ctccttttca cccgctgcat aaacatctgc aagataaaga
     2941 gggcttatgt ttttaaatac accgacaaat tcatcaaata aattttttgt tcttgaatat
     3001 ctgtgaggct ggaatattac ttttagcttt ctgggtgaaa atgaattgag tacggcttta
     3061 attgtcacat ctatttcttt tggatgatga ccgtaatcat ccacaactgt aataccgttt
     3121 actattcctt taacttcaat ccttctttct attccgccga atttttcaag tcccttattg
     3181 atatcatcaa acgacattcc caactcataa ccgattgcaa aagcaacgag agaatttaga
     3241 gcattatgaa ttcccggaag ctgaatatgt accagcccct ccagtttatt ccccctataa
     3301 agattatatg atgtctttag ataactaaac tcaacatctc ttgcttgata acaagaatct
     3361 ttaccaagac cataagtaac gtatctcttg ttatatgatt ttaacagttt catggctctt
     3421 ttgttgtcac cacatacgac cgcaagcccg taaaaaggta ttttatttat aaaatctgaa
     3481 aatgcatcat cgagctgctg tgagtcctta taatgatcca tatgctcatc atctatattt
     3541 gtaataactg ctatcacggg agaaagcttt aaaaatgaac cgtcactttc atccgcctct
     3601 actaccataa aacttccctt gccaagatat gcattagtac ccagtgattg aatcctgcct
     3661 cctactacca cagtaggatc aaatcctcca aaatcgagaa cagaggaaat caatgatgta
     3721 gtcgtagtct tgccatggct tccggctact gctatgccat atttaagcct cattagctcc
     3781 gagagcattt cagctcttgg aatcaccggg gtgccaagct tatttgcttc aattatctca
     3841 ggatttttat cggttattat tgatgaatat acgacaacat ccctaccacg cacatttttt
     3901 gctttatggc caattgaaac ctgagctcca agatcttcca ggcgctctat cactttagag
     3961 gttcttatat ccgaccctgt taccgtgtat cccatattca acatgacttc ggctattccg
     4021 ctcattccta ttccgccaat ccctacaaaa tgtatttttt ttatacgtcc gtacatggtt
     4081 gtaaatttaa tagttcat
//
LOCUS       DBNGSV010000025         3032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_834522, whole genome shotgun sequence.
ACCESSION   DBNGSV010000025 DBNGSV010000000
VERSION     DBNGSV010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_834522"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC159U_01085"
     CDS             <1..371
                     /locus_tag="AC159U_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335683.1"
                     /translation="KRGGSSELQGVVDKNKSHRGSRTNKKKLPTAGTHMSIDAESEIE
                     FKSPNLGSSEAEIDYRMITLAKSIEEKQVLLEEEFCRIYGRVINHAVARGKLPRDASV
                     KCSFAFPSFNIRDIEAEARS"
     gene            complement(479..862)
                     /locus_tag="AC159U_01090"
     CDS             complement(479..862)
                     /locus_tag="AC159U_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HIR3335684.1"
                     /translation="MPSEPLNELELTLPVDGSPMLFHMSGEFGKPSIEDKDAIVLTIN
                     QTTGNIIGETKLMVRIRKNANNLSSAERDRFISALATLNDRGMGKYSDFRNIHTDAGE
                     DEAHGRPGFLPWHRAFILDLERKQN"
     gene            complement(1470..2333)
                     /locus_tag="AC159U_01095"
     CDS             complement(1470..2333)
                     /locus_tag="AC159U_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3335685.1"
                     /translation="MGRIERILSKVDSFNETGRFEEAIKELNKAIDIAPDDPDVLLSF
                     ALTYDAMNDLDTSVTYFHKAIEQCPEDAYLWTQYGITLSRNNKHHKAIKIFQHALILD
                     PIYSIAKWNLGLTYRSIGHYEDAVREFKECLEIEPDSEYFKDEIHYHLGLCYFDMGWT
                     TEAISEFRKHTEIYPDDMFSHLSLGNCFFDLGWIDESVDRFRFIIEKSPAFIPAYNSL
                     ALSYAEKGWYDEALETLREALEIAPDDESVKENIDYIEGLKDNEDGLRSFIMYLLLKN
                     KLGIDSFSESN"
     gene            2556..2834
                     /locus_tag="AC159U_01100"
     CDS             2556..2834
                     /locus_tag="AC159U_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3335686.1"
                     /translation="MKRSELEKELAQEFNLQPNQCEHILDTIIDYMTEVLQTGDRIEI
                     RGFGSFFTKDYKSYNGRNPRTGETVHVSPKKLPHFRPSKELLSKLNDE"
     gene            2887..>3032
                     /locus_tag="AC159U_01105"
     CDS             2887..>3032
                     /locus_tag="AC159U_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3335687.1"
                     /translation="MPVLEILKYPNPKLKKLSTEVKKIDKTILNLLDGMAETMYEAPG
                     VGLAA"
BASE COUNT          937 a          552 c          591 g          952 t
ORIGIN      
        1 aaaaacgcgg gggttcatcc gagcttcagg gagttgtcga caagaacaag tcgcacaggg
       61 gatcaagaac taataaaaaa aaactcccca cggccggaac tcatatgagc atcgatgcag
      121 agagcgagat tgagtttaag agtcccaacc tgggctcatc cgaagccgaa atagactacc
      181 gcatgattac tcttgcaaag agcattgagg aaaagcaggt gttattagaa gaagagtttt
      241 gcaggattta cggccgtgtg atcaatcatg ctgtagcaag aggaaaactc ccacgtgacg
      301 catctgtaaa atgctccttc gcattcccat catttaacat ccgcgacatt gaggccgaag
      361 cccgctccta gtcactcgta gaacagaccg gcacgatgtc agactctaca tttaactcca
      421 agtttgggct tgattatgaa aaggaagtgg agcttttagg gggggagagt ggagagggtt
      481 agttttgttt tctctccaaa tctagtataa aagccctgtg ccaaggtaaa aatccaggac
      541 gtccgtgtgc ttcatcttca ccagcatccg tatgaatatt tctaaaatcg ctatatttac
      601 ccataccacg atcatttaat gttgccagag cagatataaa tcggtctcgc tcagcggaac
      661 taagattatt ggcatttttt cttatgcgaa ccattagttt ggtttcacca attatatttc
      721 ctgtagtttg attaatggtt aatacaattg catctttgtc ttctatgcta ggtttgccga
      781 attcgcctga catatgaaag agcatgggag atccatctac aggtaatgta agttctaact
      841 catttagtgg ttcacttggc attttattgt aaaaaactac ttgccaccat gattagcgct
      901 tttgtttttt aacttgacat taataggatt gttttggcct tgggtattca ctaactttat
      961 tgcacattcg acaggagccc atgatatata atttgcatca gtatcatcta ttctatataa
     1021 atgtatttct gattccataa taattggatt gtatattaaa tgttatatca tcaatctgtt
     1081 ttgtgctaat aatattgaat taataaaatg atgtaaagat actaaagagt cactctatta
     1141 aaagtgagag ttatgtttta gttactgtgt ctaagaatat ctattgttat atacattatt
     1201 gagtattatt taaccttagt ttcagataaa tttattttaa aaataaatat tatttagaaa
     1261 ggtgtccaaa taggtgtcca tttagtattg atttgctatg tttggctata ttttacatat
     1321 gttgacttat tcaataatta cagtggttta ggtttagtat agcttagtat agatttgcct
     1381 gcattgatcc ttggatggtt taggaatcct gcgctctatc ctgctgagct acggggactt
     1441 gtttgtgggg taagaaaaat tgttttatgt tagtttgatt ctgagaagga atcgatacct
     1501 agtttgtttt taagaagaag atacattata aatgagcgca ggccgtcttc gttgtcttta
     1561 aggccctcga tataatctat attttctttt accgattcat catcgggtgc gatttccagg
     1621 gcttcacgaa gtgtttcaag cgcttcatcg taccaccctt tttcggcata tgataaggca
     1681 agagaattat aggccggaat aaatgcaggg cttttctcaa ttatgaatct aaacctgtct
     1741 acagactcgt ctatccagcc aaggtcaaaa aagcagttgc caagggataa atgagaaaac
     1801 atatcatcag gatatatctc agtgtgcttt ctgaactctg atatagcctc agtggtccat
     1861 cccatatcaa aataacaaag ccccaggtga taatgtattt catctttaaa gtattcagag
     1921 tcaggctcta tttccaaaca ctccttaaac tctctcacgg catcttcata gtgcccaatg
     1981 gatctgtatg taaggccaag gttccatttg gcaattgagt aaattgggtc gagaattaga
     2041 gcatgctgaa aaattttaat agccttatga tgtttattgt ttcttgagag tgttatgcca
     2101 tactgtgtcc agaggtaggc atcttcgggg cattgttcaa tagctttgtg aaaatatgta
     2161 accgaagtat ccagatcgtt cattgcatca tatgtaagtg caaaggataa cagcacatcg
     2221 gggtcgtccg gggcaatgtc tattgcctta ttgagttctt ttatagcctc ttcaaatctg
     2281 cctgtttcat taaatgaatc tactttgcta aggattcttt cgattcgtcc cattcagttc
     2341 tccccatagt tttccatcaa tattatatta gaagattagt tattttgaga ataattgtca
     2401 aataaattga gtaaatattt agtgtcttag tataaaatac tctgaaaagt gaaatatttc
     2461 aatgatttta actacttaaa tgaaaaatat acttgacaaa aacttacaaa ttaactacat
     2521 tggaacttac cgatatacac caaaggggag acagaatgaa aagatctgag ctcgaaaaag
     2581 agttggctca agagtttaat ttacagccaa atcaatgcga acacatactg gacacaatta
     2641 ttgactatat gaccgaggta ctgcaaacag gcgacagaat cgagatcagg ggttttggta
     2701 gtttctttac aaaagactac aagtcctata acggcagaaa ccccagaacc ggtgaaactg
     2761 tacatgtgtc cccgaagaag cttcctcatt tcagacccag caaggagctt ctaagcaaat
     2821 taaatgatga ataatctgtc attacttagt aatagccttt gtaacttccg gatcaataaa
     2881 atatagatgc cagtactgga aatactcaaa tacccaaacc ccaagcttaa aaaactctca
     2941 actgaagtaa aaaaaattga taaaaccatc ttgaaccttc tggacgggat ggctgaaact
     3001 atgtatgaag ccccgggggt tggtcttgct gc
//
LOCUS       DBNGSV010000026         4908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1873644, whole genome shotgun sequence.
ACCESSION   DBNGSV010000026 DBNGSV010000000
VERSION     DBNGSV010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1873644"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1011
                     /locus_tag="AC159U_01110"
     CDS             292..1011
                     /locus_tag="AC159U_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335688.1"
                     /translation="MRKFVTIIIIIVSIGAGAMFIKAMLELEKEASNYLDSKQDSAVI
                     KSTENELSNTQNTDTDTDSESKKSDTSGGDDLDIRNKIYHLIDSSGLSDDIKAQPKLI
                     IGELYEDLFNDLGLTDEEIDQFSDLTIISSAEKFKYDETNNNDSKIKELIGELYYEDY
                     TGYNETLSERIRVIEYKQRLELSEIPLLPQQQQNLIELMLQDCDTKCDENIIDNSIDF
                     LDLEQVAVLEEYLSESSDSNH"
     gene            complement(1030..1596)
                     /locus_tag="AC159U_01115"
     CDS             complement(1030..1596)
                     /locus_tag="AC159U_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335689.1"
                     /translation="MSDVITDQQELESLIGFKLNEQSIHRINELRLKSGYDTFVDLIS
                     CCIGTLEIFLKERVKGRVFVTLSENYILDLEKWDKKVWMFDIEFLNKDIELHAKHLEV
                     VQGARIDEPDYNLDKIRGLLGNENYEDLVVNSIVLVEWVVTEINERRIIGSVMKDEVE
                     NMDSSNWRILVLYNTINDLADYKYSWDV"
     gene            complement(1623..1754)
                     /locus_tag="AC159U_01120"
     CDS             complement(1623..1754)
                     /locus_tag="AC159U_01120"
                     /inference="COORDINATES: protein motif:HMM:NF020917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR3335690.1"
                     /translation="MKIEKGLEPPTKDHYGLCRCGASKNKPYCDGSHSEAGFTDNEN"
     gene            complement(1764..2258)
                     /locus_tag="AC159U_01125"
     CDS             complement(1764..2258)
                     /locus_tag="AC159U_01125"
                     /inference="COORDINATES: protein motif:HMM:NF018595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HIR3335691.1"
                     /translation="MADEKTTIDIMENGPFIVDNLDVLENSKGEKIESKKKIALCRCG
                     ASENKPFCDGTHSKIDFTGKRESSTKLDKEIEYEGKDIKVYYNPAFCYHAAECVGNLP
                     AVFDVNIKPWINPDKSDADSIISTIKKCPSGALSYKIGDEHLRDFGGEQKIKIEKDGP
                     YSVC"
     gene            2420..2803
                     /locus_tag="AC159U_01130"
     CDS             2420..2803
                     /locus_tag="AC159U_01130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3335692.1"
                     /translation="MSNNRFETGKQTFEKITGEVGKKFLENLRTVAPDFADYLVEFPF
                     GAIYSRPGFDIKTREIITLSSLATQGNATIELKAHIKLALNAGVTREQIIELFMQISV
                     YAGFPVAVNAMLAAKDVFKELDRKK"
     gene            complement(2804..3979)
                     /locus_tag="AC159U_01135"
     CDS             complement(2804..3979)
                     /locus_tag="AC159U_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR3335693.1"
                     /translation="MKRIILYTGKGGVGKTTVAAATALKCANLGYKTIVVSTDPAHSL
                     RDSFNKELGPEPKKIGDNLYGQEIDVYYSVEKYWGKLTEYLHSLLGWMNVDDILAEEF
                     SVFPGMEEISAFLWVNNHYHDADYDVIIVDSAPTGETLRLLSLPDVARWWLVKIFPIE
                     KRVMKVIKPAVRVVSDMPLPQEATYDAIEILFEKLDAIRQVFADPKVTSMRLVMNLEN
                     MVIKETQRAYTYLNLYGYPVDSLIINRTMPEELDHPYFKRWRKKQGKYREQINDMFSP
                     LPAFDVSLFSEEIQGLASLEKFSDELFKDADPTEIFFSEKQYEIIKKNGNFEMQLKLP
                     FVSKKEVELFQVGDELTIHIENQKRNIFLPNFLAKLKVKKANFDGDVLKVLFEKKKV"
     gene            4109..4255
                     /locus_tag="AC159U_01140"
     CDS             4109..4255
                     /locus_tag="AC159U_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335694.1"
                     /translation="MSEQPNKSKVKYDPIMTPLFTILGLKILGATALLTIAGIYYSFI
                     NPSF"
     gene            4299..4853
                     /locus_tag="AC159U_01145"
     CDS             4299..4853
                     /locus_tag="AC159U_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335695.1"
                     /translation="MVKTSKVSQRESQDLNQEFQHLIASCKKSINRKVDPNLEKTLKK
                     LNKKIQSEVKSSKEKKDRDTLIYLKFLTKVTKKFKKANNILKSLKKTDSNFEELDTLT
                     ELDILLSHLKKIVNKSPKKINVKKLEADIEKIEKIEDQKVRVLMTYLMDDWKNLECVL
                     SLMKLGETYKDIADDLLSISLFKN"
BASE COUNT         1629 a          923 c          797 g         1559 t
ORIGIN      
        1 gagagattac aacgagtaca gacctcattc ggcacttgat aatatgacgc cggaggagta
       61 tgttaaacaa cacataaaaa agccggaaat ctctaattat cccggtacag cttttgggtg
      121 agggtcaatt acctaaaatt ctaacttata atctggtact attatatggg gcaaggtcag
      181 ggtggggtat atttataatt ctttttagca ctaattattt aacacattaa aaaaatttat
      241 tactattaag atagaaagat atttatattg tttttatttg gaatataaat cgtgcgtaaa
      301 tttgtaacaa taataattat tatagtttca atcggtgctg gagcaatgtt tataaaagca
      361 atgttggaac tggaaaaaga agcatccaac tatctggata gcaaacagga tagtgctgta
      421 ataaaatcta ctgaaaatga gttatccaac actcaaaata ccgatactga tacagattca
      481 gagagtaaga aatcagatac atcaggtggt gacgacttgg atataagaaa taagatatac
      541 cacctgatag acagttcggg tttgtctgat gatataaaag ctcagccaaa actaattata
      601 ggtgaactct atgaggatct attcaatgat ttgggtctta cagacgagga aatcgatcag
      661 ttttctgatc tcacaattat atctagcgca gaaaaattta aatatgatga gacaaacaac
      721 aacgacagta agataaaaga gttaattggt gagctttatt atgaagatta tacggggtat
      781 aatgaaactt tgagtgaaag aattagggtt atagaataca aacagaggtt agaactatcc
      841 gaaatacctt tacttcctca acaacagcaa aaccttatag agctaatgct gcaagactgt
      901 gatacgaagt gtgatgaaaa tattattgat aattcaatag attttctgga tttagaacaa
      961 gttgccgtac tcgaagaata tttgtctgaa tcttcagatt ctaatcacta aatctttcaa
     1021 tcaaacctac tatacatccc agctgtattt ataatcagct aaatcattga tagtgttata
     1081 cagaaccaga attctccagt ttgatgagtc catgttctcg acttcgtctt tcattacaga
     1141 acctattatt cttctttcat ttatttctgt aacaacccat tctacaagta ctatggaatt
     1201 tacaaccaga tcttcataat tttcatttcc gagcagacct cttattttat caagattgta
     1261 gtccggctca tcaattcttg ccccttgtac tacttctaaa tgtttggcgt gcagctctat
     1321 atccttgttt aaaaactcta tatcaaacat ccagactttc ttatcccact tttccagatc
     1381 taaaatatag ttttcactaa gtgtaacaaa aaccctgcct ttgacccttt ctttaagaaa
     1441 aatctctaat gttccaatac aacagctaat gagatcaaca aaagtatcat aaccgctttt
     1501 taacctaagc tcatttattc tgtgaatact ctgctcattt aatttaaagc ctataagaga
     1561 ctcaagctcc tgctgatcag taattacatc gctcatagaa ttataaaata tttttataaa
     1621 agttaatttt cattatctgt aaatcccgct tcggaatggc ttccgtcaca gtatggcttg
     1681 ttcttggaag ccccgcatct gcaaagacca taatgatcct ttgttggagg ttcaagacct
     1741 ttttcgattt tcaattctat atttcagcag acactgtatg gaccgtcttt ttcgattttg
     1801 attttctgct caccaccaaa atctcttaaa tgttcatccc caatcttgta acttagcgca
     1861 cccgaaggac atttttttat ggtgcttatt attgaatccg catctgattt atctgggttt
     1921 atccatggtt tgatgtttac atcaaaaaca gctggcaggt ttcccacaca ttcagctgca
     1981 tgataacaaa atgcgggatt ataataaacc ttgatatctt ttccttcata ctctatttct
     2041 ttatcaagtt tggtactgga ctctcttttt cctgtaaaat ctatctttga atgggtgccg
     2101 tcacaaaaag gtttattctc tgaagcaccg catctgcaca gggctatttt ctttttggat
     2161 tcaatttttt caccttttga attctcaagc acgtccaggt tatctacaat aaaaggtccg
     2221 ttttccataa tatctatcgt tgttttttca tcagccatat tattctcctt tgtagtaatg
     2281 gaatccgcta tatcaaaata atgttatcat ataagtcgct atagtcatgc taaaattaaa
     2341 tatgtaatca gcaaattccc attttccaac aaatagatat catttagtaa gatttaataa
     2401 aatataaagg agtataaatt tgagcaataa cagatttgaa acagggaagc aaacatttga
     2461 aaaaattact ggtgaagtag gcaaaaagtt tttagagaac cttagaacag tagcgcctga
     2521 ttttgcagac tatctggttg aatttccctt tggggcaata tacagccgtc ccgggtttga
     2581 tattaagaca agggagatca taactctttc ttctcttgca actcagggaa atgccacaat
     2641 tgaactgaag gctcatatca agcttgccct aaatgcaggg gtaaccaggg agcagataat
     2701 agaactattt atgcagatat cagtttacgc aggctttcca gtcgctgtaa atgcaatgct
     2761 tgcagcaaag gatgtgttca aagaactgga caggaagaag taatcagacc tttttctttt
     2821 caaataacac ctttaaaaca tcaccgtcga agtttgcttt ttttactttt agttttgcca
     2881 gaaagttggg aaggaatatg tttctctttt gattttctat gtgaattgta agttcatctc
     2941 ccacctgaaa aagctctact tctttcttgc taacaaatgg aagtttgagc tgcatctcaa
     3001 aattgccatt tttcttaata atttcatact gtttctcact aaaaaatatc tcagtcggat
     3061 cagcatcttt aaaaagctca tcggagaact tctcaagaga agcaagccct tgtatctctt
     3121 ctgaaaacag tgatacatca aaggcaggca gtggagaaaa catatcattt atctgctctc
     3181 tgtatttgcc ctgctttttt cgccaccttt taaaatatgg gtgatcgagc tcttctggca
     3241 tggttctgtt aataatcaga gaatccacag gatagccata aagatttaaa taagtgtatg
     3301 ccctctgagt ttcctttata accatgtttt cgagattcat tacaagacgc attgaggtta
     3361 ccttgggatc ggcaaatacc tggcggatgg catcgagttt ttcaaacaat atttcaatcg
     3421 catcatatgt agcctcctgg ggaagcggca tatccgaaac gacccttact gcaggcttta
     3481 ttaccttcat cactcttttt tctattggga atatctttac aagccaccac cttgctacat
     3541 cgggcagaga aaggagcctc aatgtttcac cagtaggcgc gctgtctacg atgatcacat
     3601 catagtccgc atcatgataa tgattgttaa cccacaaaaa ggcggatatt tcttccatac
     3661 ccggaaacac gctgaattcc tcagcaagaa tgtcatcaac gttcatccaa cccagcagtg
     3721 aatgcagata ctcggtaagc tttccccagt acttttcgac agagtaataa acatctattt
     3781 cctggccgta aagattgtct cctatttttt taggctcagg gcctaactcc ttattaaaag
     3841 aatctctgag gctatgggca ggatctgtgg aaactactat agttttatat cccagattag
     3901 cacattttag cgctgtagca gccgctactg tggtttttcc aaccccgcct ttacccgtat
     3961 atagtattat tctcttcatg ctactcacag taaactgtgt aaaatattaa acaataatat
     4021 tatgctctaa tctatttatc gtagttactt gacatcaatc acccagtaaa tatattatca
     4081 cttattatac ttaaaaaggt agagaaaaat gtcagaacaa ccaaataaat caaaagtaaa
     4141 atacgacccg atcatgactc ctctttttac tatactggga ttaaagatat taggagctac
     4201 tgcacttctt acaattgcag ggatttacta cagttttata aacccatcgt tttaatttct
     4261 atatattaca cactaagctt aagataaata acgtgggtat ggtaaaaact tcgaaagttt
     4321 cacaaagaga atctcaagat ttaaaccagg agtttcaaca cctaatcgcc tcttgcaaaa
     4381 aatcaatcaa cagaaaagtt gaccccaatc ttgaaaaaac actaaaaaag ttaaacaaga
     4441 aaattcaaag tgaagttaaa agttccaaag agaaaaaaga tagagatacg ttaatttatc
     4501 taaaattttt aacaaaagta accaagaagt ttaaaaaagc taataatata ctaaagtcgt
     4561 taaagaaaac tgattctaac tttgaagagt tagacacatt aacagaactt gatattttgc
     4621 tttcacacct taaaaaaata gtaaacaaat caccaaaaaa aataaatgta aaaaagctcg
     4681 aagccgatat tgagaaaatt gaaaaaatag aagaccaaaa agtaagggtg cttatgactt
     4741 acctgatgga tgactggaaa aacctggagt gcgtcctgag ccttatgaaa cttggtgaga
     4801 catataagga tatagcagac gatcttttga gtatttctct ttttaaaaac taaaagacac
     4861 ctaccctttg tacaatttct cctttagact ctttatcctt taaatctt
//
LOCUS       DBNGSV010000027         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_627242, whole genome shotgun sequence.
ACCESSION   DBNGSV010000027 DBNGSV010000000
VERSION     DBNGSV010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_627242"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            805..2190
                     /gene="ltrA"
                     /locus_tag="AC159U_01150"
     CDS             805..2190
                     /gene="ltrA"
                     /locus_tag="AC159U_01150"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR3335696.1"
                     /translation="MKKEELSKLPVSTKQQRIAEIAQRKSGEPITLLHHYIDENWLRT
                     AFFQLRKESAPGVDGETVLEYGKNLDSNVKSLLKRAKTGTYKAPAVRRSMIPKPGKRK
                     EFRPLGIPTTEDKVLQKAVLMVLEPIYELEFYGFSYGFRKGKSQHQALDYLWHEIMYK
                     NIQWIIDLDIRKFFDTVKHEILRQLLHKRVRDGVITRLIGKWLKAGVLEDGSIHYSDE
                     GTPQGGIVSPMLSNIYLHEALDKWFAQVVQPRLRGRSLIVRFADDVILGFENKAEAEK
                     VLSALTKRFEKYGLELHPEKTRLVCFRRSGKSSDSDSDGDSDEGENNKGPKGRSFDFL
                     GFTHYWGKSLKGSWVVKRKTSSKKLREKLRKMNEWCKKHRHFPIEYQYKKLCQKLKGH
                     YAYYGITGNMRGMQRYLDKVKRIWKKWLCRRSWKNKLNWEKFNIFLTQRCPLPPARVV
                     HSIYAKRAAKQ"
     gene            2323..>2543
                     /locus_tag="AC159U_01155"
     CDS             2323..>2543
                     /locus_tag="AC159U_01155"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3335697.1"
                     /translation="MKIKREIFIGNVGIGGHHPVSIQSMTSTDTHDPRATLTQIIELK
                     NAGCEIVRAAVPDREALAPLARIIKESPLP"
BASE COUNT          801 a          526 c          653 g          563 t
ORIGIN      
        1 aattagaaca cgaagacaca caaagaagaa aagattaaac aaattcaatt ggccaaaatt
       61 caactattat tttattttct tcgtgtaact tcgtgtacct tcgtggctaa aaataaaaag
      121 aaaaaaacct ttcgtgtacc ttcgcggcta aaattagaat tgcttggcgc caggtaagcc
      181 caggaagagg ggatgcgcca ggcacgtaag tgctgaaact cttcatagac acctagtggg
      241 tccgaatccc accggtaaag gctgaccacc agccggaagc gagtcttgcg tagtcgtcag
      301 taatgccggc acgaagcgta gacagcgggt acaaaagccg tgtgattgag ccacgaaatt
      361 aatattatcg atggagcctt cgtagtagca atcgcggggg cagcactgaa acgctcgagt
      421 ggtctggcgt ggaagtccgt ccgtggtcct ggagcggggc agatgtacac cagggtgtcc
      481 tgggaacctg ggagatccag tttcctcctt cttaaaagga ccgggtaagg ggctgccggt
      541 atatccaacc cctggcattg ctgcattggc tccaatgtaa caatgagcct gaagaaggga
      601 cacaaaccga tggagcgcaa gaaggtattg taagacgtaa tcaagtttta cgagacggga
      661 acctggaagt cttagctctc taatagtacc gtttgaaagc agggaaaccc accccaggga
      721 agcctgtgag tagggaagga gagagtcggg ttattgaccc gatgttggga aacacatctt
      781 acacaaggag gtaagaaaat ggttatgaaa aaggaagagt tgtcaaaact tccagtgtca
      841 acgaaacagc agcggatagc ggaaattgcg caacgtaaga gcggagaacc gataacttta
      901 ctgcaccact acatcgatga aaactggctc cggacagcct tcttccagtt acggaaggag
      961 agtgctcccg gagtagacgg agagacggtg ttagaatatg gcaagaatct ggatagcaat
     1021 gttaagtctc tgttaaaacg tgctaaaaca ggaacttaca aagcaccagc agtacgtaga
     1081 tcaatgatac ccaaaccagg gaagaggaag gaattccgac cgctgggaat tcccacgacg
     1141 gaagataagg tgctgcagaa agcggtatta atggtacttg aaccgatcta tgaactggaa
     1201 ttttacggat tttcgtatgg attcagaaaa gggaaatctc aacaccaggc gctcgattat
     1261 ctctggcatg agataatgta taagaatatt caatggataa tagaccttga tatccgaaaa
     1321 ttcttcgata cagtaaagca cgaaatactc cggcaattgt tgcacaagag ggtgcgcgat
     1381 ggtgtgatta cacgtttaat aggtaaatgg cttaaggcgg gagtgctgga agacggtagc
     1441 atacactaca gtgatgaagg tactcctcaa ggtggaattg tctcaccgat gctcagtaat
     1501 atctatcttc atgaggcatt agataagtgg tttgcgcagg tagtacagcc aaggcttcga
     1561 ggacgcagtc tgattgtgag atttgcagat gatgtgatac ttggatttga aaataaggca
     1621 gaagcagaaa aagtgttgtc ggcccttacc aaaagatttg agaagtatgg cctggaattg
     1681 caccccgaga aaacacggct tgtgtgcttt agaagatcag gaaaatccag tgacagtgac
     1741 agtgacggag atagtgacga gggcgaaaac aacaaaggtc caaaagggag aagctttgac
     1801 ttcctgggat ttactcatta ctggggcaaa tcattaaaag gtagctgggt tgtcaagaga
     1861 aagacatcga gcaagaagct gagagagaaa ttgaggaaga tgaacgaatg gtgcaagaag
     1921 caccgtcatt ttcctataga ataccagtac aagaagctat gccaaaagct aaaaggtcat
     1981 tacgcctatt acggaatcac aggaaatatg cgaggaatgc aacgatatct ggataaggta
     2041 aaacggatat ggaaaaaatg gttatgccgc agaagctgga aaaataaatt aaactgggaa
     2101 aaatttaata tatttctaac tcaaagatgc ccactcccac cagccagagt tgtacactcc
     2161 atatacgcga aacgcgcagc gaaacaataa ttcgaggaac cgtgtgcgtt aattgcgctc
     2221 gcacgggtct gcgggggtgc cagtgggcaa ctgctggcac tacccggagc cttccaaccg
     2281 tttttctttt actttttcgt tgatttttca tataatagct ccatgaaaat caaacgggaa
     2341 atttttatcg gaaatgtcgg catcggcggt catcacccgg tctccattca atccatgacc
     2401 tccacggaca cccatgatcc ccgcgccacc ttaacccaga tcattgagct gaaaaacgcc
     2461 ggctgtgaaa tcgtccgtgc tgctgtaccc gaccgggagg cattggcacc gctggcaaga
     2521 attataaaag aatcacccct ccc
//
LOCUS       DBNGSV010000028         2530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_280929, whole genome shotgun sequence.
ACCESSION   DBNGSV010000028 DBNGSV010000000
VERSION     DBNGSV010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_280929"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..2154)
                     /locus_tag="AC159U_01160"
     CDS             complement(25..2154)
                     /locus_tag="AC159U_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HIR3335698.1"
                     /translation="MASENKDAKRILSLVDYIGGDYQNAVADGKIINTDEYNEMLDFS
                     ATALELGSRIITDKEYVKNDTRLLSNLINDKADVQSVVNLSNKIKQNLISDFNLKTYP
                     DKAPSIKVGKMLYANNCSQCHGLTGMADGQLADGLVPHPANFAEGDLIEGLSAFKVYN
                     TASFGISGTGMPSFPNLTEEQKWDIAFYVLSIRYENDSQYLASTVDIPQDLNDHKVLA
                     TISDDDIRGSLGTRPYNENQIKNIIAGIRTHSHLSNGINSDNETLILTSSLLKESISL
                     YEKGDKELAYSKAIDAYLEGFEKIENKLAVKDNKLTRDIENKFGEYRGQIKSGEPLQT
                     VKTTYQQLNSGLNDASILLTNTKPIGKFLSFVQSFAIIVREGLEAILIVAAIIAFLTT
                     TGSRKAIKYVHYGWISAIVAGLGTWVIAQTVISITSAQREIIEGVTSLLAAAVLFYVS
                     YWFITKIEVQKWKEFIEGKVKQALTKKNVITLASVSFFAVYREAFETVLFYQALWLQA
                     ENTQSEVIWGLLVGTVFLIGIFIVIFKLGLKMPLRQFFAISSGLLYLLSFVLVGKGIR
                     EFQEAGIIGITPLQFIPNIDILGIYPTLQTTAPQAVLFLAFAFAILWIFVIKQERERK
                     EIVVSVTRIAEDMKTMHEAFDHIKGHITEWKRCEDIDLEAEDLDKQIHEVITHVDELE
                     TKLVDFFDVVSKNTDEESAPDTQPKYN"
BASE COUNT          774 a          492 c          427 g          837 t
ORIGIN      
        1 tgcaaaaata attagaaggg caaattagtt gtattttggc tgtgtgtcgg gggcagattc
       61 ttcatctgtg ttttttgata ctacatcaaa aaaatctaca agctttgtct caagctcatc
      121 cacatgggta ataacttcat gtatctgttt atccaggtct tcagcctcga gatctatgtc
      181 ttcacacctt ttccattctg tgatatgacc cttaatatga tcgaaggcct cgtgcatggt
      241 tttcatatcc tcggcaatcc gggttacact tacaacaatc tcttttctct ccctctcctg
      301 ctttatcaca aatatccaca atattgcaaa tgcgaatgct agaaagagta ctgcttgagg
      361 tgcggttgtt tggagtgtgg gataaattcc cagtatgtct atgttgggaa taaactgtag
      421 tggtgtaatt cctataattc ctgcttcctg aaattctctt atgcctttgc ctactaaaac
      481 aaatgaaagt aaataaagca gaccactgga tattgcaaaa aactgtctga gtggcatttt
      541 aagtccgagt ttgaatataa ctataaagat accaattaga aaaacggtac caacaagcag
      601 accccatatt acttctgact gagtgttctc tgcctgaagc cacagggcct gataaaaaag
      661 tacagtctcg aaggcttctc tgtacactgc aaaaaatgat acgcttgcaa gtgtgattac
      721 gttctttttg gtaagagcct gttttacctt tccttcaata aattccttcc atttctgaac
      781 ctctattttt gttataaacc agtaactaac ataaaaaagc acagctgccg ccaaaagaga
      841 cgtaacacct tcaattattt ctctttgagc gcttgtgatt gatataacag tctgagcaat
      901 aacccatgta cccagtcccg caacaatagc agaaatccag ccgtagtgga catatttaat
      961 ggcttttctt gaacctgtag ttgttagaaa agcaatgata gcagctacta tcaaaattgc
     1021 ttccagccct tccctcacta taattgcaaa agactgaaca aaagatagaa atttaccaat
     1081 tggctttgta tttgttagaa gaatagatgc atcattaagt ccggagttta gctgctgata
     1141 agtagtctta acagtttgca gaggttcacc gctctttatc tgtcctctat attcaccaaa
     1201 tttattttct atatcacgcg taagtttatt atctttaaca gcaagcttgt tttctatctt
     1261 ttcaaatccc tcaagatagg catcaatcgc tttcgagtat gcaagctctt tgtcaccttt
     1321 ttcatataaa ctaatagatt cttttaataa gctgcttgta agaatcaaag tctcgttatc
     1381 actatttatg ccattggata aatgggaatg agttcttatt cctgcaataa tattttttat
     1441 ctgattttca ttataaggtc gtgtaccaag actaccccta atgtcatcat cagatattgt
     1501 agcaaggact ttatggtcgt ttaagtcctg tgggatatca actgtagacg caaggtattg
     1561 agagtcattt tcatatctta ttgaaagcac ataaaaagca atatcccact tctgttcttc
     1621 agtaagatta ggaaacgaag gcatgcctgt accactaata ccaaaacttg cagtattata
     1681 gactttaaat gcagagagac cttctattaa atcaccttct gcgaagtttg ccggatgtgg
     1741 tacaagacca tctgccagtt gaccatctgc catacctgtt agaccgtggc attgagaaca
     1801 gttgttagca taaagcatct tgccaacttt aattgaaggt gctttatccg ggtatgtttt
     1861 caggttgaaa tcagaaataa ggttttgctt gattttattg gataggttta caacgctttg
     1921 aacatctgct ttatcgttaa taaggttgct taacagccgt gtatcatttt ttacatattc
     1981 tttatcagtt ataatacgac ttccaagttc taaggcggtt gcagaaaaat cgagcatctc
     2041 attatattca tcagtattga tgatttttcc atctgctaca gcgttttgat agtcgccacc
     2101 aatgtagtca actagagaca ggattctctt ggcatccttg ttttctgatg ccacagatgt
     2161 catcggtatt agaaaaacta agctacatac tatataaagt actactcgta acctgttata
     2221 tttacagatt ttatttaaaa tcaatttaaa tccccactat tatgtatata caatgtccag
     2281 attataaata agaagcgaat tcataagtca aaaacagagg ttaccatgag cctaatacct
     2341 gtcaagacgg tgttatttat gaccagaatc atcataatat agtagcggaa tttgataaaa
     2401 aacaaggagc tcgcatagaa cagagctata agtaatgcag aatctccttt attttattaa
     2461 ctaaatctac atttctgtta ttaaatctaa attccatctc ttttagatac aaaacaaagt
     2521 gttttctgtc
//
LOCUS       DBNGSV010000029         4482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1873923, whole genome shotgun sequence.
ACCESSION   DBNGSV010000029 DBNGSV010000000
VERSION     DBNGSV010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1873923"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="AC159U_01165"
     CDS             <1..1358
                     /locus_tag="AC159U_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3335699.1"
                     /translation="VVEKEGIQTRHISGLGGTRVLWSSLKKGEIDIYPEYTGTLIQEI
                     FSDRKIESTDQLRSELHKLGIGMTDPLGFNNTYILGMKKEKAAKLNIATISHLVKHPD
                     LKIGFSSEFIERADGWPGLKRAYNLTHKNIRGLDHDLAYRGLNSGSIDVIDLYSTDAE
                     IDYYNLAALEDDLNFFPQYFAVILYRLDTNNIYPEAIAKITSLENNISEKFMVGMNAE
                     VKIAGVAESTVASDYIKKTFSVTTRPAESTIFSRFIKNTFDHLVLVCISLFSAIIISI
                     PLGILSYKNEPIGRIILGFVGIVQTIPSIALLVFMIPLFGIGFIPAVIALFLYSLLPI
                     VRNTYSGLKDVPVEIKESAEAIGLADGARLKLVELPIASRSILSGIKTSAVINVGTAT
                     LAALIGAGGYGQPIFTGIRLDNIGLILEGAVPAAVLALLVQALFGLLEKVFIPKGLRL
                     N"
     gene            1422..2327
                     /locus_tag="AC159U_01170"
     CDS             1422..2327
                     /locus_tag="AC159U_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335700.1"
                     /translation="MRNTMALIILIALFSFACGSENTSSAENMSFGDYWYKGKAEITS
                     YKLQQARYGQINSGDAVLIFVTEDFSNSKQVKLDNLQSVPDDAVKVLKLNSTRKFLTG
                     IYPYSIMTSVFSPVDFVSKPNAMKLTFSSQEWCGHVYTQANHKNNKYVISSYSYFESE
                     GDAKTELDDALLEDELFTKIRLSPDSLPTGKIKIIPSLLFSRLKHFDLKVEDALAEKV
                     ASDSTNDLVSYKVKYKNLQRTVRIDFLNKFPFEIIGWSEEYKSGFGANAKMLTTTATN
                     HKSIMLDYWNKNSLDDEDLRKQLGL"
     gene            complement(2375..2788)
                     /locus_tag="AC159U_01175"
     CDS             complement(2375..2788)
                     /locus_tag="AC159U_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3335701.1"
                     /translation="MSLGIRLFEPFIDNAFGSLLNKDYYDEGRLSPSVDVYEKSDKYV
                     INAEIPGLSKEEIKIDVKDGTLTISGEKKYEEKKEDENYIRVERRYGKFERRFNLPEN
                     VNVESVTANYKNGVLEVSLPKKVEAKPKQIDVKVS"
     gene            2976..3548
                     /locus_tag="AC159U_01180"
     CDS             2976..3548
                     /locus_tag="AC159U_01180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3335702.1"
                     /translation="MCLFIKQFFTKPLSTGAIHPSSDELAELITDTAELKKRNLIVEI
                     GSGTGVFTQKICEKKNDNAGFFVIELNPLFVEQTKRRCPAVNVVEDSAANINLYLEKQ
                     GYKSCDCIISGLPWASFDNNMQDSILQNLYNSMTPGAVFLTFSYVQSLLMPSGRRFKN
                     KLNSMFGNVEQTKVIWKNVPPAFVYWVYKS"
     gene            3584..>4482
                     /locus_tag="AC159U_01185"
     CDS             3584..>4482
                     /locus_tag="AC159U_01185"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3335703.1"
                     /translation="METTKQDKKVIIIGGGPAGLTAAYELNKHGIRSEVFEKDNIVGG
                     ISRTVNYKGYRFDIGGHRFFTKVKVVQDMWDEVLQNEFLLRDRLSRIYYKRKFFSYPF
                     EVKNAVFGLGIFNSMTIVLSYLKAQIFPIKPENTFEHWITNRFGYRLYKIFFKTYTEK
                     VWGIPCSQISSEWAAQRIRGLSLFSALKNALLKQPAANKDVIKTLINSFHYPKMGPGM
                     MWEKVRDLVEQGLSTVHMNSGVDKIYWEDGKIVSIFSENTKSVQGTDFISSMPIRELI
                     AKLDPAAPKDIIEASQKLNYRDF"
BASE COUNT         1480 a          765 c          858 g         1379 t
ORIGIN      
        1 aagtcgtaga aaaggaagga atacaaacca gacacataag cggccttgga ggtacaagag
       61 ttttatggag ctctcttaaa aagggtgaga ttgatatcta tcccgaatat acaggcacac
      121 ttatacaaga gatttttagt gacagaaaaa tagagagtac cgatcagcta agatcagaac
      181 ttcataagct tggaatcggc atgactgacc ctctgggatt taataatact tatattctgg
      241 ggatgaaaaa agagaaggca gcaaaactca atattgcaac tatttctcat cttgtaaaac
      301 atccggattt aaaaattgga ttttcaagtg aatttataga aagagcagac ggatggccgg
      361 ggcttaaaag ggcttataat cttacacata aaaatataag aggtcttgat cacgaccttg
      421 cctacagggg ccttaacagc ggaagtattg atgtgatcga tctttattcc acagatgctg
      481 aaattgatta ttacaacctt gctgcgttgg aagatgattt gaactttttc ccccagtact
      541 ttgcagtaat actctacagg ctggatacca ataatattta tcctgaagca atagctaaaa
      601 taacttcatt agagaataat atatccgaaa aatttatggt ggggatgaat gcagaagtaa
      661 aaatagcggg tgttgctgag agtactgtgg cctcggacta tattaaaaaa actttttcgg
      721 taactacaag acccgccgaa tctacaatat tttccagatt tataaaaaac acatttgatc
      781 atcttgtact ggtttgtatc tcactttttt ctgcaataat catctcgata ccacttggaa
      841 tattatcata taagaatgaa cccattggca gaattatatt gggatttgtg ggcattgtgc
      901 agaccattcc atcaattgca ctgctggttt ttatgatccc tctatttgga ataggtttta
      961 taccggcagt tatagcgctt tttttataca gtttacttcc catagtacgc aacacctata
     1021 gcggtcttaa agatgtaccg gtagaaataa aggaatctgc cgaggccatt ggtcttgcag
     1081 acggagcaag gttaaagctt gttgaacttc ctattgcatc caggtccatt ctctcaggaa
     1141 ttaaaacatc tgctgttatt aatgtgggaa ccgcaacact tgctgcgctt ataggtgctg
     1201 gaggatacgg ccagcctata tttacaggca tccgtctgga caatattggg cttatacttg
     1261 aaggtgctgt acccgctgct gttctggctc ttttagtcca ggcgctattc gggttgctgg
     1321 aaaaggtatt tattccaaaa ggtttgagac ttaattaata ctattcaaat tattttggtt
     1381 atcctatttt aattacagac aattaacaaa agaggaaata tatgagaaat acaatggctc
     1441 taataatact aatagcactt tttagctttg cctgcggaag tgaaaataca agcagtgccg
     1501 aaaatatgtc atttggcgac tactggtaca agggaaaggc agagatcacc agttacaaat
     1561 tacagcaggc aagatatgga caaataaata gtggtgatgc ggtactgatt ttcgttacag
     1621 aagatttttc taattcaaaa caggtaaaac ttgataacct gcaaagtgtg cccgatgatg
     1681 cagtaaaagt actaaaactt aactcaacca gaaaatttct tacaggcatt tatccctatt
     1741 cgataatgac ctctgtgttc agccctgttg acttcgtatc aaaacctaat gcaatgaaac
     1801 taacattttc ctctcaggaa tggtgcggtc atgtatacac acaggcaaat cataaaaaca
     1861 ataaatatgt aatcagttca tattcctatt ttgaatccga gggtgacgca aaaactgaat
     1921 tagatgatgc cctactcgaa gatgagctgt ttacaaagat aagactatct cctgatagcc
     1981 tgcccacagg aaaaattaaa attattccct cacttctttt ttcaagacta aaacattttg
     2041 atctaaaagt agaggacgct ttagccgaaa aagttgcctc tgattctaca aatgatttag
     2101 tttcctataa agtgaaatat aaaaacctgc aaagaacagt aagaatagat tttctaaata
     2161 aatttccttt tgaaattata ggatggagcg aagaatataa gagcggattt ggagcaaatg
     2221 caaaaatgtt aacaacaact gcgactaatc ataagtctat tatgctggat tattggaaca
     2281 aaaatagttt agatgatgag gatttaagaa agcaacttgg gttataaata taagctgcca
     2341 cccttaatct taagagtggc agccggaatt acttttaact aacttttaca tcgatttgct
     2401 ttggttttgc ttctactttt tttggcagac ttacttcaag cacgccgttt ttatagtttg
     2461 ccgtaactga ttctacattt acgttttcgg gtagattgaa tcttctttcg aactttccgt
     2521 atcttctttc gactctgata tagttttcgt cttctttctt ttcttcatat tttttctcgc
     2581 cgctgattgt gagtgtaccg tcttttacgt ctatcttgat ttcctcttta cttagaccgg
     2641 ggatttcagc gtttatcaca tacttatcac ttttttcgta aacgtctacg cttggagaga
     2701 gtcttccctc gtcgtaatag tctttattaa gcaatgaacc aaaagcatta tcaataaaag
     2761 gctcaaataa tctaattcct aatgacattg tttttacctc ctaaacaatt tttagataat
     2821 attaaagtaa tcatccttcg atgggtttca aggggataat agagaaatat tttaaaaaaa
     2881 gtttatggat gttatatata aaacttagta taatcagtag cttaatagtt gtgggtatat
     2941 taaatcaatt ccgggcttaa tttaggatat tatcaatgtg tctttttata aaacagtttt
     3001 ttacaaaacc cctatctaca ggagccattc atccttcttc ggatgaactt gccgaattga
     3061 ttactgatac tgccgagctt aaaaaaagaa atcttatagt cgaaattggc tctggtactg
     3121 gagtatttac tcaaaagata tgcgaaaaaa agaatgacaa tgccggattt tttgtgatcg
     3181 agttaaaccc tctttttgta gaacaaacaa aaagaaggtg tcctgccgta aatgtggttg
     3241 aagattcagc agcaaacata aatctgtatt tagaaaagca gggttataag agctgtgatt
     3301 gtattatatc cggactgccg tgggcttctt ttgataataa tatgcaggac agtattttac
     3361 aaaatttata taattcgatg actccgggtg cagtattttt aaccttttcc tatgtgcaaa
     3421 gtttactaat gccgtctgga agaaggttta aaaataaatt aaatagtatg tttggaaatg
     3481 ttgagcaaac aaaagtaata tggaaaaatg tccccccggc atttgtatac tgggtttata
     3541 aatcataggt cttaatactc tctattaata ataaaacgta taaatggaaa caacaaagca
     3601 agataaaaaa gtgataatta taggcggagg tcctgcgggg cttactgcgg cctatgagct
     3661 gaataagcac ggtataaggt cggaagtttt cgaaaaggat aatatcgtag gaggtatttc
     3721 gcgcacggta aattacaaag gctacagatt tgatataggc ggtcacaggt tttttacaaa
     3781 agtaaaagta gtacaggata tgtgggatga agtattgcag aatgaatttt tactaagaga
     3841 ccgtctttcg agaatttatt ataaaaggaa attcttcagt tatccgttcg aagtcaaaaa
     3901 tgcagtgttt ggtcttggta tattcaacag tatgactata gttcttagct atctaaaagc
     3961 acagattttc ccaataaaac ctgaaaatac ttttgaacac tggatcacaa acagattcgg
     4021 atacaggctc tataagatat tcttcaaaac atatactgag aaagtgtggg ggataccatg
     4081 ctctcagata agttcagaat gggcagcaca acgaataaga ggactgtctc tgttttctgc
     4141 actaaaaaat gcactcctaa aacagcctgc tgccaataaa gatgttatta agaccttaat
     4201 aaattcattt cattacccta aaatgggccc cggtatgatg tgggaaaaag taagagacct
     4261 ggttgaacag ggtcttagta ccgtacatat gaacagcggg gtagataaaa tttactggga
     4321 agatggcaaa atcgtatcaa ttttttccga aaatacaaaa tctgtacaag gcacggattt
     4381 tataagcagc atgcccataa gggagcttat tgcaaagctt gaccccgccg ctccaaaaga
     4441 tattattgaa gcatctcaga aactaaacta cagggatttt at
//
LOCUS       DBNGSV010000030         5622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_557898, whole genome shotgun sequence.
ACCESSION   DBNGSV010000030 DBNGSV010000000
VERSION     DBNGSV010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_557898"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="AC159U_01190"
     CDS             <1..722
                     /locus_tag="AC159U_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3335704.1"
                     /translation="DSNNNSNSESTSSETMSKDLHLEDIELQNSVFQSANIRQTTESI
                     GSSSSSDLSSDSDELDKLISEIDSSIQQNYGQVIMSDFGEEAQAEDIDEQKYIVFTLS
                     NKMYGIPILNVLEIGTLSNVTPVPNLPVWVRGVTNIRGEIVSVVDLGTYLGIPKMDRF
                     DSGRMLVIRSLDEEISSAIIVDQVNQIYNVPKDSIMNTEAKVDDKIAPFLHGVCEFND
                     ELLILLDLEKFFAFPQIRQFD"
     gene            862..2673
                     /locus_tag="AC159U_01195"
     CDS             862..2673
                     /locus_tag="AC159U_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR3335705.1"
                     /translation="MNLSYKYFLLIYCISIFLCPFPVFSQDEPGEIYISKIKLNNIGA
                     VSKKEITENMATEFPSFLPWKKKPVFDEEILKEDVIRISNLYKEYGFYDVKIEYLIEQ
                     KNDDFVDVIIDIDRGEKTVVKDFVLDVKPKLLDEEYAELLDAATVMSNEDFSQREYQK
                     TKNIIANYFSNKGYPFAEIKSEALVNREDKWAKVDISVNRGYKYYFGTVSIKGNKDID
                     DKIISREILYKSGDEYSLTMLDRTRLAIFGLGYFKSVVIDPVFNEEQKVVDTVISVEQ
                     RKLGSVKVGVGFGTEDLFRGQIIWNHKNLFGGARDLEVSGKFSFLTQRLSASLIQPYL
                     FDRDMDFISTLSTSRDDFPSYTSESLNFSNKIKKKIYNNINFYSSFDIQLSRLSEISG
                     STSDFVEDDGYFLTFLSAGLDHSTVDNPLNPTSGRSLSFNIESSLGVLGSDEDFITSV
                     LEFIGYKEYKNIVFAKRLDIGLIQPFGSTDSLDVPIFKRFFTGGSTTMRGFPFQELGP
                     LDSGSEPVGGNTLLLGNLEGRFPIYKKLGGVLFFDYGNVFPDEFDFDFGEIKYAVGTG
                     LRFDTIIGPLRVDFGYTLNPEPEIDRFQFFLSIGHAF"
     gene            2663..>5622
                     /locus_tag="AC159U_01200"
     CDS             2663..>5622
                     /locus_tag="AC159U_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR3335706.1"
                     /translation="MRSKIIKIFSVLLVLLIVGIFSLSLLIKTSFVQNKIVDLIKSSV
                     SKSMGQQLKIGKVDFALIDGIVINDTELEIDNNKFLEARNITLGYPLSALNIINDFSA
                     GHINVDSFKIDGLKLVLSKNQDGIWNYSRLSRKEPKNEDEQLTQKESSSWIVSINSFE
                     ILDTRVSIFNKQADTVRTIDINDFNSTVRLATNPAAAEINFHSGRINIDPDEIIINNL
                     SSSTLKLSKEEVEIGELKFGFNGADIFMSGEFKNSGEKLFSVRTKVTDFSNEKLKLNL
                     DFKGEGDFTTTDDINGKFSIDIDNSQYLENKFSFDAGDISIKNSVLNMKNATLDGDAG
                     KAEIGIDTDLKNIFDKDKNNEFFAVLKLSDFDYSRLSKVPNIDTEIPADIEKIASALN
                     AELQISGFFNASENFDLKTDIRKFILIGEAGDANLGGVLNISDSLIVADITGSTDKLD
                     IGYLLNKKKYNSSINSKINIDASLPTNFSISGLKGSSQIDILKSRIFKNEIKSGAIRA
                     KFSPNKIENVSVKLLSDFVNINASGNAINDKLDFSYDIKSENLDFISKIKDDLGMHGS
                     ADIKGQIKGTLESPIIEYALKLSDFKVKKLIADKGNATGAISFDEGISTINSRGQFNR
                     LKYNDRPFNSLEYDVSTKNGRVIGDILLTENDNNNYNLEFKIADLELEKNIEFSKITL
                     NFGDTEFKNNDTLVVGYSNGIRISGLDLGSNGSSIKGVFNISASDELKGDIKFINFEL
                     QNISELINVQEAFSGVVNGHLDINGNTKAPRLNSQLSIDNLKYREFASSKLNLNTKYS
                     NKKFDINLTSDLQDNNKLNLSGFINTDLNFSNEKSSGVTSMDLSLFSQNLNLSPFSAF
                     IDTLDDINGVIDLDLKIKGTPKAPRVFGNTAIKDTEFKLKQLKNKITIDNGNMKFDGD
                     KAVFNEFMISSGDGTAGISGDFNLSDLSFKAQGEFDRFRINPKRIKTDLSGNLQLSGN
                     GKDISAAGDATLNKT"
BASE COUNT         1931 a          819 c         1109 g         1763 t
ORIGIN      
        1 aagattccaa taataacagc aatagtgaaa gcacctcttc agaaactatg tccaaagacc
       61 ttcatctcga agacatagag ctccaaaata gtgtttttca gagtgcaaat atcagacaga
      121 ctacagaatc tattggcagc agtagcagta gtgatttatc ttcagatagt gatgaactcg
      181 acaaactgat ttcagaaata gactcttcca tacagcagaa ttatgggcag gtaataatgt
      241 ctgactttgg agaagaagct caggcagagg atatagacga gcagaagtat attgtattta
      301 cgctgtcaaa taaaatgtat ggcataccta ttttaaatgt gctggaaata ggaactctca
      361 gcaatgttac cccggtacca aatcttcccg tatgggtaag aggtgtaact aacattagag
      421 gggagattgt ttcggttgtt gatcttggca catatttggg tattcccaag atggatagat
      481 ttgattcggg aagaatgctt gttatacgtt ctttggatga agaaataagc tctgctatta
      541 tagttgatca ggtaaatcag atatataatg ttccaaagga ttcaattatg aataccgaag
      601 caaaggtaga tgataaaatt gctccttttt tacatggagt ttgtgaattt aatgatgaac
      661 tattaatatt gcttgatctt gaaaaattct ttgctttccc tcaaataagg cagtttgatt
      721 agtatttagc gaagtaatta attgctttat tccatctctg ttgctaaact tcattgcggc
      781 tgatatttta ccttatcatt ttaatacagt ccgttattct cttaagtaat tatcggaatt
      841 gttataatat tagcttgggt gatgaattta tcatataaat atttcttact aatatattgt
      901 atctctatat tcttgtgtcc ttttcctgtt ttttctcaag atgagccggg tgaaatatat
      961 atttccaaaa tcaaattaaa taacattggt gctgtatcta aaaaagagat taccgagaat
     1021 atggcaacag aatttccatc gtttttaccc tggaagaaaa aacccgtatt tgacgaagaa
     1081 atattaaagg aagatgtaat acggatatct aatttatata aagaatatgg attttatgat
     1141 gtaaaaatag aatatctaat tgagcaaaaa aatgatgact ttgttgatgt gataatagat
     1201 atagatcgtg gcgaaaaaac ggttgtaaaa gattttgtac tggatgtgaa gccaaaacta
     1261 ttggatgaag agtatgccga gcttttggat gcagctactg taatgtccaa tgaagatttc
     1321 tcgcaaagag agtatcaaaa aacaaagaat attattgcga actatttttc aaataaaggc
     1381 tatccttttg ctgaaataaa atccgaggcg cttgtaaaca gggaagataa atgggctaag
     1441 gtagatattt ctgttaacag ggggtataaa tattactttg gcactgtatc cattaagggt
     1501 aataaggaca tagacgataa gattatatcg agagagattt tatataagtc gggtgatgaa
     1561 tattctctaa caatgctcga caggacaagg cttgcaatat ttggtcttgg gtatttcaaa
     1621 tccgttgtga ttgatcctgt atttaatgag gagcaaaaag tcgttgatac cgtaataagt
     1681 gttgaacaac ggaaacttgg gagtgtaaag gttggagtag gttttggcac cgaagacctt
     1741 ttccgaggac agattatatg gaaccataaa aatctgttcg gtggtgcaag agacttagag
     1801 gtttcaggca aattttcgtt tcttactcag cgcttaagcg cttcactaat tcagccttat
     1861 ttatttgaca gagacatgga ttttatctct actctttcta cttcaaggga tgattttcca
     1921 agctacacct cagagagcct caatttttct aataagatta agaagaaaat atacaataat
     1981 ataaattttt acagttcttt tgatatccag ctgtctcgat tatcggaaat atccggttct
     2041 acttctgatt ttgtagagga tgatggttat ttccttactt ttttaagtgc cgggctagac
     2101 cacagcacag ttgataaccc gcttaacccc acaagtggaa ggagtctgag ttttaatatc
     2161 gagtcgtctc taggggtttt agggtctgat gaagatttta ttacaagcgt tcttgaattt
     2221 atcggatata aagaatataa aaacattgtt tttgcaaaaa gactggatat agggttgata
     2281 cagccgtttg gcagtaccga tagtctggat gttcctatat ttaagagatt ttttacaggc
     2341 ggcagcacaa ctatgagggg gttccctttt caggagcttg gcccgcttga cagtggtagt
     2401 gagcccgtgg gtggaaacac acttttactt ggtaatcttg aaggaagatt tccgatctat
     2461 aaaaaacttg gaggagtgct gttttttgat tatggaaatg tattccccga cgaatttgat
     2521 ttcgatttcg gagaaataaa atatgcagtg ggcacaggtc ttaggtttga tactataata
     2581 ggccctttga gggttgattt tggttatact ttaaatcctg agccagagat tgacagattt
     2641 caatttttct taagcatagg tcatgcgttc taaaataata aagatatttt cagttttgct
     2701 ggttctacta attgtaggga tattttcatt atctctgctt attaagacga gttttgttca
     2761 aaataagatt gtcgatctta taaaaagctc cgtaagcaaa tctatgggac agcagttaaa
     2821 gataggcaag gtggattttg ctttaataga tggaattgta ataaatgaca ccgaactaga
     2881 aattgataac aataaatttc tggaagctag aaatattact cttggatatc cattaagtgc
     2941 attaaatatt attaatgatt tttcagcggg gcatataaat gtagacagct ttaaaattga
     3001 tggacttaaa ctagtactca gcaaaaacca ggacggaatc tggaactata gccggttaag
     3061 tcgtaaagaa cctaaaaatg aggatgaaca actcacacaa aaagagagtt ctagctggat
     3121 tgtttccata aatagttttg agattttaga taccagggtt tcgattttta ataaacaagc
     3181 tgatacagta agaactattg atataaacga ttttaattct acagtaagac ttgcaacaaa
     3241 ccctgctgct gctgaaataa actttcatag tggccggatt aatattgatc cggatgagat
     3301 aattataaac aatttatctt catccacact taagctcagc aaggaagaag ttgaaattgg
     3361 tgaattaaag tttggtttta atggcgctga tatattcatg tccggagaat ttaaaaattc
     3421 gggtgaaaaa ctattttctg tcagaacaaa ggtaactgat tttagtaacg aaaaactcaa
     3481 actgaacctg gattttaaag gtgaaggtga ttttacaact acagacgata ttaacggtaa
     3541 atttagtatt gatatcgata attctcaata tctggaaaac aaattctcat ttgatgctgg
     3601 tgacatatcg atcaaaaatt ctgttctaaa tatgaaaaat gctacccttg atggagatgc
     3661 cggtaaagca gaaattggca ttgatacaga tcttaaaaat attttcgata aagataagaa
     3721 taatgagttt tttgcagttc taaagttgtc tgattttgat tattcaagat tatctaaagt
     3781 tccgaatatt gatactgaaa ttccggctga catagaaaaa atagcatctg cattgaacgc
     3841 tgaactacag atctcaggtt ttttcaatgc aagtgaaaac tttgatctaa aaactgatat
     3901 cagaaaattt attttgattg gtgaggctgg cgatgcaaac ctaggcggag tcttaaatat
     3961 ttcagattct ctcattgttg cagatattac gggttctacc gataaactcg acataggcta
     4021 tttgttgaac aaaaagaaat ataatagctc aattaattca aagattaata ttgatgcgtc
     4081 attgcccaca aattttagca tcagtggttt aaaaggaagt tcccaaattg atatacttaa
     4141 atcaagaata tttaaaaatg agataaaaag tggtgcgatc cgagctaaat tttctccaaa
     4201 caagattgag aatgtatcgg taaagcttct gtctgatttt gtaaatatta acgcttccgg
     4261 aaatgccata aatgacaaac ttgatttttc ttatgatata aaaagtgaaa atttggattt
     4321 tatatccaaa attaaagatg atctgggtat gcatgggtca gctgatataa aaggacagat
     4381 taaaggtacg ttggaaagtc caatcattga gtatgctctc aagctgtctg atttcaaggt
     4441 aaaaaaattg atagcagaca aaggcaatgc cacgggcgct atatcatttg atgagggtat
     4501 ttcgactata aattcaaggg ggcaatttaa tagattaaaa tataatgaca gaccatttaa
     4561 tagcctggag tatgatgtat ctacaaaaaa tgggcgggtg atcggcgata ttctgcttac
     4621 tgagaatgac aataataatt acaacctgga gtttaagatt gccgatctgg aattggaaaa
     4681 aaatatagaa ttttcgaaaa tcacacttaa ctttggagat acagaattta agaacaatga
     4741 cacactcgtt gtaggctata gtaacggcat aaggataagc ggacttgatc tcggcagtaa
     4801 tgggtccagt attaagggtg tgtttaatat atcggcaagt gatgaattaa aaggtgatat
     4861 aaagtttata aattttgagc tgcaaaatat ctctgaatta ataaatgtac aggaagcttt
     4921 cagtggtgta gtgaacggcc atcttgatat aaacggaaac acaaaagcgc ccaggctaaa
     4981 ttctcagcta tctatcgaca accttaaata cagagaattc gcatcaagta aattaaacct
     5041 aaatacaaag tatagcaata agaaatttga tataaatctc acttcggatc tacaggataa
     5101 taacaagctt aatctctcag gctttattaa taccgatcta aattttagta atgaaaaatc
     5161 ttctggtgtt actagtatgg acttatcact attttctcaa aatctaaatc tcagcccttt
     5221 ttcagctttt atagatacat tagatgatat aaatggtgtg atcgatctgg acttaaaaat
     5281 caaaggtaca ccaaaagctc ccagagtttt cggaaataca gcgatcaaag atacagaatt
     5341 caaattaaaa cagctaaaaa ataaaattac aattgataat gggaatatga aattcgatgg
     5401 tgataaagct gtattcaatg aatttatgat atccagcgga gatggtactg cgggtataag
     5461 cggtgatttt aatttatctg atctgagttt taaggcacag ggagaattcg acaggtttag
     5521 aattaaccca aaaagaatta aaacagatct ttctgggaat ttgcagttaa gcggaaatgg
     5581 caaagatata agcgcagccg gagatgcaac tctaaacaag ac
//
LOCUS       DBNGSV010000031        18680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1112127, whole genome shotgun sequence.
ACCESSION   DBNGSV010000031 DBNGSV010000000
VERSION     DBNGSV010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18680
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1112127"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC159U_01205"
     CDS             <1..249
                     /locus_tag="AC159U_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335707.1"
                     /translation="LNRSPNADELSDMEIDHYMEQWIGMDWDCRKMLDFEASQLVPHG
                     SGITFSGRQDPVLNKYRALALMRREKACTRLVPIISAV"
     gene            complement(268..804)
                     /locus_tag="AC159U_01210"
     CDS             complement(268..804)
                     /locus_tag="AC159U_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3335708.1"
                     /translation="MKPIILITILMLFSACTKPMDQESIDPVVSKGKQLVSEGNCSYC
                     HTPLKEVNGEDVEDLDRYLSGHPESKPVPKLPDFPVDSDQWLEFLTKLDSTVWAGGWG
                     VTLSANITPDKETGIGNWTEEMFIETMRKGRHKGFGRNLQPPMPWQDYGKLSDEQLQS
                     IFAYLRSIRPIKNKVPRQ"
     gene            997..1458
                     /locus_tag="AC159U_01215"
     CDS             997..1458
                     /locus_tag="AC159U_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335709.1"
                     /translation="MNEILDKLEQLELRYSVARSYLPTLIFDVLILIAFIFIVFMIGA
                     DLKYLFFVILPIIHFSYIYFMKDNEHYLNRTVFIKIDKKGIYDYREDRDNPDFIGWGS
                     VRSISHDFLVYTTFGRTAQIPISGWDIPPNVIRKRTRYIIDSNGYDIHTDW"
     gene            1605..2981
                     /locus_tag="AC159U_01220"
     CDS             1605..2981
                     /locus_tag="AC159U_01220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3335710.1"
                     /translation="MDNNSESKAHVHPGRLPAFWDRSVIFFANLKSMFFGNIEQLGKL
                     ESEVSGVNTYGGRLITVINTLYQSGPNLLVLEKEPNKSILSYLGKTLKLSLPDILIVE
                     ADVFSAINKGTGDKEFRYKIDRAKERISSHPAGWIDGYVTDDMLVHWARKLNKSNINT
                     AEGCHIANNKLLLHYYLLEKGLPVIDTEVAQSQDKAQDCLDKLSKKGYTRAVAKSQIG
                     ASGIGMIALNTSGSNKPMPDYMFYEGPCIIQGWMKPGEGGIEKIYSPSVQLFVDDNSV
                     HLYDITEQILSKHSVHEGNIAYPPYLEDYPGLKKELLDQAIVAARWLHSQAYRGTASV
                     DYLVTIRNGELEVRICEINARVTGATYPSVLARHFIPHGAWIMRNLKTEQPIEGQKLL
                     ELLDIKGALFYPGKTEGFIPINFNLNNDGRVIKGQFLYIAENVGICSQALKNFSDALP
                     IGWEYDRD"
     gene            2968..4092
                     /locus_tag="AC159U_01225"
     CDS             2968..4092
                     /locus_tag="AC159U_01225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3335711.1"
                     /translation="MTETKNETTDSIREHLVGLTKDLVLIPGTAERPDDIERCVSFIK
                     DYLKNLDRVRINEHVQNEVPSIVALPKDITEPDILLVSHLDVISHADVGVYRPRINNG
                     RIYGPGSGDMKGAMSIMLELFHHFHSTYPGISLGLAITSDEEQGGVSGIGHLFNDVGL
                     RCGLAINPDGGSLNEITIAEKGVVQLNVICTGHSSHAARPWLGTNALEVLIERLTALK
                     QYFSQMKISGDNWYPTCALTLISTPNHTINRVPEKAEASLDIRFPPPYTVDKIVAECS
                     RILGSDVEAHVTLSAEPVEFESNPLFAKIIEEVTNNPASLIREDGASDCRFIHRYEIP
                     VIISRPLVGELHTKDEWIDIESMVVFYQICKGFIERHLNL"
     gene            4195..4617
                     /locus_tag="AC159U_01230"
     CDS             4195..4617
                     /locus_tag="AC159U_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3335712.1"
                     /translation="MIKNDNIKKILTTDVISVHVSQKLSDVNKIFRENMIHHVPVLKG
                     KKPIGIISTNDIFKLMFNIEMQDDRMLDAILDHHFTIKNAMSKDLVTLPITSTIKDVA
                     KILQFSTIHSIIITNEAGELEGIVTSTDLIKHLHDNIK"
     gene            complement(4666..5133)
                     /locus_tag="AC159U_01235"
     CDS             complement(4666..5133)
                     /locus_tag="AC159U_01235"
                     /inference="COORDINATES: protein motif:HMM:NF022338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3335713.1"
                     /translation="MKHNAAKRDLSIKIVTAIVVIMMAGFFIASIYINILIIAAVITI
                     VILIICYLYAPTAYEISDSKLIVYRNFGEIEFSNVTACRFIEDKVPFTVRLWGNGGVF
                     AGMGIFWNSRFGVFRMYVTNTKQKEFVLVETESQIVIISPENPQVFIDSWKTK"
     gene            5249..6148
                     /locus_tag="AC159U_01240"
     CDS             5249..6148
                     /locus_tag="AC159U_01240"
                     /inference="COORDINATES: protein motif:HMM:NF041383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPGUxxT family thioredoxin-like (seleno)protein,
                     type 2"
                     /protein_id="HIR3335714.1"
                     /translation="MKILIALLLALMTMLVVLKDMTEIYSVNAEVAEYKYSDDFKPNL
                     NNPAELGYINWHRNFNKAVELARTEDKPILLFFNEVPGCNTASGYGINVMRHPLITEA
                     AEALFVPVAIYNNVGGHDREVLNSFGEPTWNNPVVRFIDSDKKQLTPRLVGDYTKLGL
                     VRSMIKALKSDNKQVPEYLTILEKELSGKRSGLEKAVFSMYCFWSGEGKLGSIDGVVS
                     TKAGFMGGREVVMTKYNPQVISYEKLLKKARSEGAADNVFAVNENQKQIAKKLIGQGR
                     VSTEQTFRLDREPKYYMSKSHYK"
     gene            6155..6364
                     /locus_tag="AC159U_01245"
     CDS             6155..6364
                     /locus_tag="AC159U_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335715.1"
                     /translation="MTPLQASLVNSAIGNKKSPNKYLSQRQLGILKRVKDNPEAGWKD
                     HTASDDFIAEWNHTVGKLDAVVSKK"
     gene            complement(6388..7797)
                     /gene="pyk"
                     /locus_tag="AC159U_01250"
     CDS             complement(6388..7797)
                     /gene="pyk"
                     /locus_tag="AC159U_01250"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3335716.1"
                     /translation="MNNSLRKTKIICTIGPATSSFEMLEKMYHAGMNVVRLNMSHGTH
                     ESHEKVIRSIKSINQKIKDSIPVLLDTQGPEIRTGNLQNDLHLQDGDEISISVRPENV
                     EQSSFHINYEDLTNTVEEGDMITVDNGLINLQILRKDEGALHCKVIDGGRLTSKRHVN
                     LPGIRVNLPSITEKDKADIMFGIEQEVDFIALSFVREANDVLQLKELLDKKANKIKII
                     AKIEDRQGVSNIEEILDVSDAIMVARGDLGIEINIEELPNVQRTIVRKCHEKGKRVIV
                     ATHLLESMIENPVPTRAEVTDVANAIYEEVDAVMLSGETTVGKYPIRCIEHLDKIARA
                     SEKLPGLNFSSNLIKTDNRQHLAATAVGHAEAIDAKGIVVITRRGITANFVTNCRPFK
                     THIYAFTNDNRTRRQLFLNRSVMAHRISFSSDPEKTIQTALGVLKKREGFLAGDLVVV
                     ISDIIAESGISSIQTRSVV"
     gene            complement(7847..8752)
                     /locus_tag="AC159U_01255"
     CDS             complement(7847..8752)
                     /locus_tag="AC159U_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3335717.1"
                     /translation="MELHQLRYFISIVETGSFSKAARRCHIAQPSLSQQIIKLERELK
                     SKLFDRLGNKVVLTEAGTALLPRAKRIMQEALKVKADIADERSIDTGCISVGFITTIA
                     PYLLPESLKKFKKKFPNADIKVYENLTENLVEKLIAFEIDIAVMSLPIENALVGYNEL
                     FIDPLLLSVPKSFKLSKQKKVSINDLKNVSFIELDEQHCLGEQIKGICYQQQMTPDTV
                     CKTWNISTILNCVATGTGIAIIPLMTALTNKSKSYVYKKLSENPSRAVVSAFYKKRTR
                     KELIIEFEKIIVEVYKDLIHKNPKY"
     gene            8865..11078
                     /gene="katG"
                     /locus_tag="AC159U_01260"
     CDS             8865..11078
                     /gene="katG"
                     /locus_tag="AC159U_01260"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIR3335718.1"
                     /translation="MGKKNTISESKCPFSGTPAKHGAGGGTSNRDWWPNQLKLNILRQ
                     NSSLSNPMGEEFNYAKEFKSLNLKAVKKDIFDIMTTSQDWWPADYGHYGPLFIRMAWH
                     SAGTYRIADGRGGGGSGTQRFAPLNSWPDNANLDKARLLLWPVKQKYGKKLSWADLMI
                     LAGNCALESMGFKTFGFAGGREDVWEPEEDIYWGSEAQWLGDKRYSGDRELENPLGAV
                     QMGLIYVNPEGPNANADPLSAAHDIRETFGRMAMNDEETVALIAGGHTFGKTHGAGNP
                     DKYVGPEPAAAGIEEQGLGWKNTLGSGNAENTITSGLEGAWTTTPTKWSNNFFENLFE
                     YEWELTKSPAGAQQWRPKNGKGKGAIPDAHNPKKKHAPFMLTTDLALREDPIYGPISR
                     RFYENPDEFADAFARAWYKLTHRDMGPRARYLGKEVPDEVMIWQDPLPAVTHKLINNK
                     DITALKSRILASGLTVSQLVSTAWSSASTFRGSDKRGGANGGRIALAPQNNWKVNKPA
                     QLAKVLKALKAIQTKFNKSQKGGKSVSLSDLIVLGGCAAIEQAAKNAGHNIKVPFTPG
                     RADATQKQTDVESFAVLEPVADGFRNYVSTGCSVPAEEMLVDRAQLLTLTAPQMTVLL
                     GGMRVLRTNSDSSKNGVFTKRSEALTNDFFVNLLDLGITWKATSKAQDLFEGSDRKAG
                     KTRWTGTRTDLIFGSNAELRAIAEVYGSKDSEKRFVQDFVTAWNKVMNLDRFDLS"
     gene            complement(11152..12855)
                     /locus_tag="AC159U_01265"
     CDS             complement(11152..12855)
                     /locus_tag="AC159U_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335719.1"
                     /translation="MHKFLLLAFAIIFASCAAKDTAPPEAEVDKKPVQQATTDLSKLG
                     TVNFQTSCDPAAAVHLNRGLALLHHMTYGLANKEFQTATSVDPQCAIGYWGQAMTGIH
                     PLWSDKPSENVFNNGKSLIDKASASMNKSGWEADYIAATGQYFSPGWTADEKANLQGL
                     EKGWESVYLKYPDDLEARSLYALSFLANADPQDKSYARQLSTGQLLERVLEEVPNHPG
                     AHHYTIHAFDYPPLAEKALDIAYNYGNIAPDIPHALHMPTHIFTRLGLWDDSIEMNSR
                     SAAAALKNPAGGAISLHYLHALDYMAYSYLQKGQDLKAKEVLDTIAALDGPIQKHVVS
                     SYTLASVSARLAMERHDWQQASQVQAQTPGDYPIGSHPAMFAITHFTRALGAANTGQE
                     ELAKEEIAILGRLQEEESKSSAYWAKQVEIQQLSAVAWLTYNQGLKEQGLNVMKKAAD
                     LEATTDKHPITPGEILPASELYGDMLAKSEKYSQALKQYERTLVRSPNRFNSLLGAAQ
                     MAELSGDKGKAFWYYTKFVELTKDAEIQLDEVDKAKTFIGMIEKKKTEINNKKNKQSE
                     Q"
     gene            complement(12964..13428)
                     /locus_tag="AC159U_01270"
     CDS             complement(12964..13428)
                     /locus_tag="AC159U_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3335720.1"
                     /translation="MNKKLSNMETRAWIGFVKAQHTVLEKVDRDFKASGFPPLSWYDV
                     LLELEKAKGGRLRQKELGELILLSKYNVSRLLDRLEKEGLIKRMPCKEDTRGVFAVIT
                     AKGKKLRNNMWPVYYDSIKEHFTSKLSKDDLEHITGVSEKLVNISISRFKSD"
     gene            13524..13859
                     /locus_tag="AC159U_01275"
     CDS             13524..13859
                     /locus_tag="AC159U_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3335721.1"
                     /translation="MIERINRQGVVDLIGKPESAVLVEALPEKYYNDAHIPGAVQMDY
                     TEVTDKAPSVLGDKDRKIVVYCASSECENSTKAARTLQQLGYDRIYVYKEGKQDWIEA
                     GLRVESAGE"
     gene            13902..14339
                     /locus_tag="AC159U_01280"
     CDS             13902..14339
                     /locus_tag="AC159U_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3335722.1"
                     /translation="MIGKLMHTDSNDTGSLIARVVAGAVMLPHGLQKLLGMFGGYGFS
                     ATLDFFASSGLPKLIGFLIIMGESVGAVMLIVGLFSRFAAASLTIIMLGAIKIHLENG
                     FFMNWFGNQNGEGFEFHILMVGLLLIVAIKGSGKWSVDSILTK"
     gene            14409..14876
                     /locus_tag="AC159U_01285"
     CDS             14409..14876
                     /locus_tag="AC159U_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335723.1"
                     /translation="MTRPRLIISFLLLGLILNPFAVSSEEKKYKAFTDIGPIEKSDLT
                     ITAIFSDKDKYHREVLTVEGTVAKLKYKKMFNGRKFTYFSLADSENNTINVYARGYVK
                     DINEGSEIRIYGRYSKEKSFLFKKRKNVMKARKIEILDQSESVVLQTEQKTQP"
     gene            complement(14927..16483)
                     /gene="pckA"
                     /locus_tag="AC159U_01290"
     CDS             complement(14927..16483)
                     /gene="pckA"
                     /locus_tag="AC159U_01290"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3335724.1"
                     /translation="MSNSEVGKRVAKKHGITKPGNIYYNLPIPTLYEEFIKRNEGTIG
                     EGGTIVAHTGKHTGRSPQDRFIVQDSKNKNTINWGKVNKPFDSRKFDALLNRVLKHLN
                     GKDLFVRDLFACAHDDYKMPVRVINEYAWHNIFVNSLFLRPDDDKYSHKKVEFTVISS
                     PSFKANPKRDGTRTETFIILNLSKRIALIGGTEYAGEMKKSVFSALNYLLPEQNVFPM
                     HCSANVGQKGDVSLFFGLSGTGKTTLSADPKRPLIGDDEHGWFDKGVFNFEGGCYAKA
                     IKLSPKTEPEIYNASLKFGSVVENVEFDPDTKRIDFDSIKYTENTRVAYPIHHLDCIV
                     PEGVGGVPKTIFMLTYDAFGVLPPISKLTLKQAIYFFIIGYTAKVAGTERGVTEPQVT
                     FSSCFGAPFLPRHPKFYADMLHKKLKKNRANVWLLNTGITGGPYGVGKRMPLPETRAL
                     VTAALDGSLSKAKFTEVPIFGLNVPTRCPGVKKETLQPRLTWKSTKKYDEKAAELQAM
                     FDAEFAGHDL"
     gene            complement(16630..17523)
                     /locus_tag="AC159U_01295"
     CDS             complement(16630..17523)
                     /locus_tag="AC159U_01295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3335725.1"
                     /translation="MNISHKKLVNFKAKDGFEVNAVLTICKKRNEKELYNTPIIIQVH
                     GVLGNFLTRGTPRLLPPALFDVGIHSLSINSRMAHMGQIIDSAIFDETIYDIDAAVRY
                     LVKIGFHRIFILGYSLGANLTAHYASVNTHRNVKGVILEGCSYSLPESQKDRLQKWNS
                     IPGYEDIYEKAEHLLSPNPLKSINDRIFVVYRAWGPTFNPIDCEMFSYKTWWFMRSPQ
                     AENAKTYKLIDKIKVPVLFINGKNDDIVFAWEPKDLKKILERAGNSNVKLRFIPDAKH
                     DCMENPKATISAISGFVKKIK"
     gene            complement(17551..18450)
                     /locus_tag="AC159U_01300"
     CDS             complement(17551..18450)
                     /locus_tag="AC159U_01300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3335726.1"
                     /translation="MKYMEQELIAIPFGSSKVDALYYKSKKNVSEEISKTAVIHVHGF
                     LGNFLDGTQRFLPPILAKSGYSSLSINTRLANFGLFFGYGIIDDTIPQIDRSIEFLLE
                     QGFKNIVVSGYSLGGAVALRYMAMRNSEKKYPHIKGVIVLAAPYSMPDSIKRRWDKWG
                     SEPSYDQIYKEAEAIFKGDPDNTDEDRTIVIYKTRGDSYIPEHSGIYTYKTWWHLASP
                     HAESGKCYNQIEKIKLPILLVQGWYDEVVSSQETYHLAQVARDAGNKSVSVFFVDSGH
                     AFEGNEKELGDIAVGWLNSKFEE"
BASE COUNT         5443 a         3889 c         3878 g         5470 t
ORIGIN      
        1 ttaaacagat cacctaatgc agatgaatta tctgatatgg aaattgatca ttatatggag
       61 cagtggatag ggatggactg ggactgtaga aaaatgctgg attttgaggc aagccagtta
      121 gtcccccatg gaagcggcat cacgttttca ggcaggcagg acccggtgtt aaataaatac
      181 cgcgcgcttg cacttatgag aagagaaaag gcatgcacaa ggttagtacc gattatatca
      241 gcagtttaga agagatataa aattatatca ctgccttgga actttatttt taataggcct
      301 tatagaccgc agataagcaa atatggattg cagctgctca tctgagagct tgccgtaatc
      361 ctgccagggc atcggtggct gcagattcct gccaaacccc ttgtgcctgc ccttgcgcat
      421 ggtctcaata aacatttcct cagtccagtt tccaatgcca gtctctttat caggagtaat
      481 gtttgctgaa agcgtaactc cccagcctcc tgcccatacg gtggaatcca gctttgtaag
      541 aaactcaagc cactgatcag agtctacagg gaaatcaggc agcttcggta cgggtttact
      601 ttctggatgt ccagataaat acctgtccaa atcttcaaca tcctcgccgt ttacctcttt
      661 tagaggagta tggcagtaag agcagttgcc ctccgagaca agctgttttc ctttggagac
      721 taccggatcg atagattcct gatccatagg ctttgtgcag gctgaaaaaa gcatgagtat
      781 tgttatcaag attattggtt tcatgttttt aaagaataca taataaaatt aagatacata
      841 tattctctct gatatataaa atatctcaag ataataatca gatgaaaaac acaaaagtaa
      901 taaggcgctt tatcaaatta aacaggtcgt tattacatcc cctattaaac cctcccattc
      961 caaacgggat gtttatgtga taaattttat ggagtaatga acgaaattct ggacaaactg
     1021 gagcagttgg agcttaggta tagtgtagca cggtcttatc ttcctaccct gatttttgat
     1081 gtgcttattt taattgcatt tatattcata gtcttcatga taggcgcaga cctgaaatat
     1141 cttttttttg taatattacc cattatccat ttttcttata tctatttcat gaaagacaat
     1201 gagcactatc tcaacagaac ggtatttatc aagatagaca aaaaaggcat ttacgattac
     1261 agagaagata gagacaatcc cgacttcata ggctgggggt cggtacgcag tatttcccat
     1321 gatttcctgg tatatacaac ctttggaaga acggcccaga taccgatcag cggctgggac
     1381 atccctccaa acgtaatacg caaacgcacc cgctatataa tcgacagtaa cggctacgat
     1441 attcacacgg actggtaaga ctttaattat attctattat gaaataacag gcttaacaga
     1501 gtttgctgaa ttattattca tcctgccatg attttttaag acatattttc ccaaacagta
     1561 ctccaatctt atacatttaa tatttatccg gtaatatttt tttaatggat aacaactcag
     1621 aatccaaagc tcatgttcac cccggaaggc tacccgcatt ttgggaccgt tctgttattt
     1681 tctttgccaa tcttaaatca atgttttttg gaaacataga gcagctggga aagcttgaga
     1741 gtgaagtgtc tggggtgaat acgtatggag gaagactcat tactgtaatc aacacactgt
     1801 atcaaagcgg gccaaacctt ctggtattgg aaaaagagcc taataagtca atactgagct
     1861 acctgggaaa aaccttgaaa ttaagcctgc ccgatatatt gattgtggaa gccgatgtat
     1921 tttctgccat caataaaggc accggtgata aagagttcag gtataagatt gacagagcaa
     1981 aagaaaggat atcttctcat ccggcaggct ggattgacgg ctacgtgaca gacgacatgt
     2041 tagtacactg ggcccgtaag cttaataaat ccaatatcaa caccgctgag ggatgtcaca
     2101 tagcaaacaa caagcttctt cttcattact acctcctgga aaaaggcctg cccgttatcg
     2161 atacagaagt tgcgcagtcg caggataaag cacaggactg tctggataag ctctcaaaaa
     2221 aaggctatac gagagcagtt gcaaaatccc agatcggcgc atcgggcata ggcatgatcg
     2281 ccctaaatac atcgggaagc aataaaccaa tgccagatta catgttctat gagggaccgt
     2341 gtattattca gggctggatg aaacccggag agggcggtat cgagaagata tattctccaa
     2401 gcgtgcagct gtttgtggat gataattccg tgcatctata tgacataaca gaacagatac
     2461 taagtaaaca cagcgttcat gagggcaata tcgcatatcc cccttatctg gaagactacc
     2521 ccgggcttaa aaaagagctt ctcgaccagg ccatagttgc tgcaaggtgg ctgcactccc
     2581 aggcttaccg cggcactgcc agcgtggatt atctggtgac aataagaaat ggtgaacttg
     2641 aagtgcgaat ttgtgaaata aacgcccggg tcaccggagc cacatacccg tccgtgcttg
     2701 caagacactt catccctcat ggggcatgga tcatgagaaa cctaaagaca gaacagccca
     2761 tagagggaca aaagctgctt gagcttctgg acattaaagg cgcgcttttt tatccaggaa
     2821 agaccgaggg atttatacct attaacttta acctcaacaa cgatggaaga gtaataaaag
     2881 gccagttctt gtacattgca gaaaatgtgg gtatttgttc tcaagcactt aaaaatttca
     2941 gcgatgcgct tccaatagga tgggaatatg acagagacta agaatgaaac cacagacagc
     3001 atacgcgagc atcttgtggg ccttacaaaa gaccttgtcc taattccggg cacagccgaa
     3061 agaccggatg atattgagcg atgcgtctca tttataaaag actatcttaa gaatctggac
     3121 agggtacgaa taaacgaaca cgttcaaaat gaagtaccgt ctatcgtggc gcttccaaaa
     3181 gatatcacag agccggatat acttctcgtc tcacacctgg atgtaattag ccacgcagat
     3241 gtaggcgttt atagacccag gataaataat ggaagaatat acggcccggg aagcggcgac
     3301 atgaaagggg ccatgtctat tatgctggag ctcttccacc acttccacag cacctacccc
     3361 ggcatatcac tcgggcttgc cattacatcc gatgaggagc agggaggagt gagcggaatt
     3421 ggtcacctgt ttaatgatgt gggcttaagg tgcgggcttg ccataaaccc cgacggagga
     3481 agcctcaatg aaattacgat tgccgaaaag ggagtggtac agcttaatgt tatttgcacg
     3541 ggtcattcaa gccacgcagc caggccctgg ctcggcacaa atgcgcttga ggttttaata
     3601 gaaagactaa cggcgctaaa acagtatttc tcccaaatga aaataagcgg cgacaactgg
     3661 tatccgactt gtgcactcac actcataagc accccaaatc acactattaa ccgcgttccc
     3721 gaaaaggccg aggcgagcct tgatattcgt ttcccgccgc cctatacggt ggataagata
     3781 gtcgccgagt gttcgaggat attgggcagt gacgtcgaag cgcatgttac actttctgct
     3841 gaacctgtgg aatttgaatc aaatcccctg tttgctaaaa tcatagaaga ggttacgaat
     3901 aatcctgcaa gcctgatcag ggaagacggt gccagcgact gcaggtttat ccaccgttac
     3961 gaaatccccg taataatatc aagacccctt gttggtgagc ttcatacaaa ggacgagtgg
     4021 atagacattg aatccatggt tgtgttttat cagatatgca aagggtttat cgagcgccat
     4081 ctgaatttat aagttataag actgattgta ttgttcaagc tgagttattt atgtatttta
     4141 taaagaaata ttcatattag cattgcttct ctataaaaat caggaggtcc aactatgata
     4201 aaaaatgaca acattaagaa gattcttaca acagatgtga tttcagttca cgtaagtcaa
     4261 aaactaagtg atgttaacaa gatttttaga gaaaacatga tacaccatgt accggtgtta
     4321 aaggggaaaa aacctatagg cattatatct accaacgata tttttaagct catgtttaat
     4381 atagagatgc aggatgatag aatgctcgat gcaattctgg atcaccattt cacaattaaa
     4441 aatgcaatgt caaaggattt agtaactctt cccatcacat caacgataaa ggacgttgcc
     4501 aagattttgc agttcagcac aattcattcg attataatca ccaatgaggc tggcgaactc
     4561 gaaggtattg ttacaagtac agatctcata aaacatttac atgacaatat aaagtgattg
     4621 ctacttgtaa accaggatta atacggactg gcttttgaag ctcagttatt ttgttttcca
     4681 cgagtcaata aacacctgcg gattttcagg tgagataatt acgatttggc tctctgtttc
     4741 cactaaaaca aattcttttt gctttgtgtt tgtaacatac atcctgaata ctccaaaacg
     4801 actattccag aatatcccca tgcctgcaaa caccccgccg tttccccaca accttactgt
     4861 gaacggcact ttatcttcta taaagcggca ggctgtaaca ttgctaaatt ctatttcccc
     4921 gaaatttcta taaacaatga gtttgctgtc cgatatttca tacgcagtgg gtgcataaag
     4981 gtagcaaatt attaatatca caatagttat cacagcagca atgatcaata tatttatata
     5041 tatagatgca atgaaaaaac ctgccatcat aattaccact atggcggtta cgatcttgat
     5101 agataaatcc cttttagcag cattatgctt catatagtta ttttacctta tatagggttg
     5161 gtaaaagaat attcaagaat attttgaacc tgtttattag ttgctataat cagatatacg
     5221 agagatttta aatggcggtg aaataaagat gaagatatta atagctttgt tactggcgct
     5281 tatgacaatg cttgtagtgt taaaagatat gactgaaatt tattctgtaa atgcagaagt
     5341 tgccgagtac aaatatagtg atgactttaa gccgaatctg aataatcccg cagagcttgg
     5401 gtatataaac tggcatagaa actttaataa agctgtcgag ttagccagga ccgaggacaa
     5461 accaatcctt ttatttttca atgaagtacc cggatgcaat acggcatcgg gttacggcat
     5521 aaatgtaatg agacaccctc taattacaga agcagcagag gccctgttcg ttcccgttgc
     5581 gatatataat aatgttgggg gtcatgacag agaggtgctt aattcctttg gcgaacccac
     5641 atggaacaac cctgtagtaa gatttataga ttcggacaaa aaacagctca ctccaaggct
     5701 tgtcggtgac tacacaaaac ttgggcttgt gcgctctatg ataaaagccc tcaaatcaga
     5761 taacaagcaa gtgcccgagt atttaacaat ccttgaaaag gaactctctg gcaaaaggtc
     5821 tggactggaa aaggcagtat tttctatgta ctgcttttgg agtggagaag gcaaacttgg
     5881 cagtattgac ggagtggtat ctacaaaggc aggctttatg ggcggcagag aagtagtgat
     5941 gaccaagtat aatccgcagg ttatttcata tgaaaagtta ctaaagaagg caagaagtga
     6001 gggtgcagca gacaatgtat ttgcagtaaa tgaaaaccaa aagcagatcg ctaaaaagtt
     6061 aattggacaa ggcagagtgt caactgagca aacattcagg cttgaccgcg agccaaagta
     6121 ttatatgtcc aaatcacatt ataaataggt gcctatgacc cctcttcagg cctcacttgt
     6181 aaattctgca atcggaaaca aaaaatctcc aaataaatac ctgtcacaaa gacagcttgg
     6241 tatattaaaa agagtcaaag ataatccgga agctggatgg aaggatcata ctgccagtga
     6301 tgattttata gcagaatgga accatactgt tggtaaactg gatgccgtag ttagcaaaaa
     6361 atagagtttt agcagactaa attagcatta aacgacagac cttgtctgaa tagaagatat
     6421 acccgattct gcaattatgt ctgatatcac gaccacaaga tcacctgcta aaaaaccctc
     6481 ccttttcttt aataccccaa gggctgtctg aatagtcttt tcaggatcgg agctaaaaga
     6541 gatgcggtgc gccataacgc ttcggtttaa aaagagctgc cttctcgtgc ggttgtcatt
     6601 tgtaaatgca tatatatgtg tcttaaaggg tctgcaattg gtaacaaaat tagcagtaat
     6661 accgcggcgc gttattacaa cgataccctt tgcgtcgatg gcttcagcat ggcccacggc
     6721 agttgcagca aggtgctgtc tgttgtcggt ctttataaga ttgctgctaa aattaagccc
     6781 aggcagtttt tccgatgccc ttgcgatttt gtccaaatgc tctatacatc ttataggata
     6841 ctttcctacc gtagtctcgc cggagagcat cacggcatcc acttcttcat agattgcgtt
     6901 tgcaacatca gtaacctctg cccgggtagg cacggggttt tcaatcatcg attcgagcag
     6961 gtgagtagca acgataacgc gctttccttt ttcatggcat ttccttacta tagtgcgttg
     7021 tacgtttggc agttcttcaa tatttatctc tattcccaga tctccccttg caaccataat
     7081 cgcatctgat acatcgagga tttcctctat gttgcttact ccctgcctgt cttcaatttt
     7141 tgcaatgatc ttaattttat tggccttctt atcgagaagc tcctttagct ggagcacatc
     7201 gtttgcttct cttacaaaag aaagtgcaat aaaatcaacc tcctgctcga taccaaacat
     7261 tatatcggcc ttgtcttttt ctgtgattga cggaaggtta acccttatac ccggaaggtt
     7321 aacatgccgt ttgcttgtaa gccttccgcc gtctatcacc ttacagtgca gtgccccctc
     7381 gtctttgcgc agaatctgaa gattgataag cccgttgtcc acagtgatca tatcaccttc
     7441 ttccactgtg tttgtaaggt cttcatagtt tatatgaaat gaagactgct ctacattttc
     7501 cggccttacg gatatggaaa tttcatcccc gtcttgcaga tgcaggtcgt tttgcagatt
     7561 gcccgtgcgt atctcagggc cctgagtgtc cagcagaacc ggaatagaat ctttaatttt
     7621 ctgattaatg ctttttatcg atctaataac cttttcatga ctctcgtgag tgccgtgaga
     7681 catattaaga cgcaccacgt tcatccccgc atgatacatc ttttcaagca tttcaaagct
     7741 gctcgttgca ggccctatgg tacagatgat ctttgtcttt ctgagtgaat tgttcattgt
     7801 tttttgaatt aaattattcc ctgtatttaa ttaatgtaaa attatcttaa tattttgggt
     7861 ttttatgaat taagtctttg taaacttcga ctatgatctt ttcaaattct atgattagct
     7921 ccttccttgt tctttttttg taaaatgcgc ttactacagc cctggatggg ttttcggaaa
     7981 gtttcttata aacataactt ttggatttat ttgtaagcgc tgtcataaga ggaattattg
     8041 ctattcccgt gcctgttgct acgcagttga gtattgtact tatattccat gttttgcaaa
     8101 ctgtatcggg cgtcatctgt tgttgatagc aaataccttt tatctgttca cccaggcagt
     8161 gctgctcatc aagctcaatg aaggaaacgt ttttaagatc attgattgac actttttttt
     8221 gctttgaaag cttaaaactc tttggcacag agagcaaaag tgggtcaata aaaagctcat
     8281 tgtaccctac caaagcgttt tctatgggaa gactcataac agcaatatca atttcaaatg
     8341 caataagttt ttctacaaga ttttcggtca gattttcgta aacctttata tcggcatttg
     8401 gaaacttttt cttaaacttt tttaatgact caggcagcag atacggtgca atagttgtga
     8461 tgaaaccaac agatatacat cctgtatcaa tgctcctctc atcagctata tctgctttta
     8521 ccttcaaagc ttcctgcatg attcgttttg cccgcggaag aagagctgtg cctgcctcgg
     8581 tgagcacgac tttatttcca agcctgtcaa atagttttga cttaagctcc ctttcgagct
     8641 ttattatctg ctggcttaga gaaggctggg ctatatggca ccttcttgca gctttactaa
     8701 aactgcccgt ttcgacaata gagataaaat atcttagttg atgaagctcc atagctaaat
     8761 attactatag gtattaccta tttcaaatat tatatattta tatttgttat ctatagcttc
     8821 aaacgcaata atatagtatc aatacaattt gaggagaaaa ttaaatggga aagaaaaaca
     8881 caatttcaga aagcaagtgc ccgttttcgg gtactccggc caagcatggt gccggcggag
     8941 gcacatcaaa ccgtgactgg tggccaaacc agctaaagct aaacatcctt cgccaaaatt
     9001 cttctttgtc taaccctatg ggtgaggaat tcaattatgc aaaggagttt aagagcctta
     9061 acctaaaggc tgtaaaaaaa gacatatttg acattatgac aacttcacag gactggtggc
     9121 cggcagacta tggccattac ggaccacttt ttattcgtat ggcctggcac agcgcaggca
     9181 catacagaat tgcagacgga cgcggtggag gaggatcagg cacgcagcgc tttgccccgc
     9241 taaacagctg gcccgacaat gctaacctcg ataaggcccg ccttctgctt tggcccgtta
     9301 aacagaaata cggaaagaaa ctttcgtggg cagaccttat gattctggcc ggaaactgtg
     9361 cgcttgaatc gatgggcttt aagacatttg ggtttgccgg agggcgtgag gatgtttggg
     9421 agcctgaaga ggatatttat tggggaagcg aagcgcagtg gcttggagac aagcgttact
     9481 ctggtgacag ggaacttgaa aatccgcttg gtgctgttca gatgggcctt atatatgtaa
     9541 accccgaagg tccgaatgca aatgcagatc cgctttcagc agcccatgac attcgcgaaa
     9601 catttggccg aatggctatg aacgatgaag agaccgtagc tctgattgcc ggcggacaca
     9661 catttggaaa aacccacgga gccggaaatc ctgataagta tgttggcccg gaacccgcag
     9721 ctgcaggcat cgaagaacag gggcttggct ggaaaaacac acttggaagc ggcaacgcag
     9781 aaaatactat tacaagcggc ctggagggag catggactac tacacccaca aagtggagca
     9841 acaacttctt tgaaaacctt ttcgaatatg aatgggagct tacaaagagc cccgcaggag
     9901 cgcagcagtg gagacccaag aatggaaaag gaaaaggagc catacccgat gcgcataatc
     9961 ccaagaagaa gcatgcgcca ttcatgctga ctacagacct ggcgcttaga gaggatccaa
    10021 tatacggtcc tatttcaaga cgcttttatg aaaatcccga tgagtttgca gacgcttttg
    10081 caagagcatg gtacaagctc actcacagag atatgggccc ccgcgcacgc taccttggca
    10141 aagaagtgcc tgatgaagtg atgatctggc aggacccttt accggctgtt actcataaac
    10201 ttattaataa taaagacatc actgcactaa aaagcagaat tcttgcatca ggactcaccg
    10261 tttcacagct tgtatccaca gcctggtcat cggcttcaac attccgcggt tccgataaac
    10321 gcggcggggc aaacggcggg cgtattgcac ttgctcctca aaacaactgg aaagtaaaca
    10381 aacctgccca gttagcaaaa gtgctaaagg cactaaaagc cattcaaact aagttcaaca
    10441 aatctcaaaa aggaggcaag agtgtatcac tttctgacct tattgttctt ggcggatgtg
    10501 cagcaataga acaggctgca aaaaatgccg ggcacaatat taaagttcca tttacaccgg
    10561 gcagggcaga tgctacgcaa aagcagaccg atgtggaatc atttgccgtg cttgaaccgg
    10621 tagccgacgg atttcgtaac tacgttagta ccggatgctc agtgcccgca gaggaaatgc
    10681 ttgttgacag ggcacagtta ttaacactca ccgctcctca gatgacggtt cttcttggcg
    10741 gtatgcgcgt gcttagaact aactcggaca gttcaaaaaa cggcgttttt actaaacgct
    10801 ccgaagcgct aacaaatgac ttttttgtta accttctcga tctgggtata acctggaagg
    10861 caacctccaa ggcccaggat ctgtttgaag gcagcgaccg caaggcagga aagaccaggt
    10921 ggactggtac acgtaccgac cttatttttg gctctaacgc agaactaagg gccattgctg
    10981 aagtctacgg aagtaaagac tctgagaaac gattcgtaca ggactttgtt acagcatgga
    11041 ataaggttat gaatcttgac cgctttgacc ttagttgata tctgatattt ggtatttaaa
    11101 tgctgattga acaaaaaaag tgggggatgc aaaaagcatc ccccttaact attattgttc
    11161 ggattgtttg ttcttcttat tatttatttc tgtctttttc ttttctatca tgcctataaa
    11221 agtttttgcc ttatctacct catcaagctg tatttcggca tcttttgtaa gctctacaaa
    11281 tttggtgtaa taccagaatg ccttaccctt atcacctgag agttcagcca tttgtgcagc
    11341 acccaacagg ctgttaaatc tgttaggact tcttacaagg gttctttcat actgcttgag
    11401 cgcttgtgag tatttctcag actttgcaag catatctcca taaagctcac ttgcaggaag
    11461 aatttctccc ggagtaatag gatgcttgtc ggtcgtagcc tcgaggtcgg ctgctttttt
    11521 cattacgttt agtccctgct ctttaagacc ctgattatat gtaagccatg ccacggccga
    11581 gagctgctga atctcaacct gctttgccca gtaggcagag ctttttgatt cctcttcctg
    11641 taacctgcca agaatagcga tctcttcttt agcaagctct tcctgtcccg tatttgctgc
    11701 gccaagcgct cttgtaaaat gagtgatggc aaacatagca gggtgtgaac ctatgggata
    11761 atcgccaggg gtctgcgcct gtacctgtga ggcctgctgc cagtcatggc gctccattgc
    11821 aagtcttgca gagacagaag caagagtata ggagcttaca acatgttttt gaatcgggcc
    11881 atcaagggct gctattgtat ccagtacttc tttagctttt aggtcctgac ctttttgcag
    11941 gtatgaataa gccatgtagt caagggcatg aaggtagtga agagaaattg cgccccctgc
    12001 cgggttttta agagccgctg ccgctgatct tgagttcatt tcaattgaat cgtcccaaag
    12061 gccaagcctt gtaaaaatgt gtgtaggcat atgaagtgcg tgtgggatat ccggtgcaat
    12121 atttccgtag ttataagcta tatccagtgc tttttcagca agcggcggat agtcaaacgc
    12181 gtggattgta tagtgatgag ccccggggtg gtttggtact tcctccagca ctctttcaag
    12241 gagctgtccg gtgcttaact gtcttgcgta ggacttgtcc tgagggtctg catttgctag
    12301 aaatgaaagt gcatacaggc ttcttgcctc aaggtcatcg ggatatttca ggtacacaga
    12361 ttcccaccct ttttccagcc cctgcagatt tgccttttca tcggcagtcc agccggggga
    12421 aaaatactgc ccagtagctg caatatagtc tgcttcccat ccagatttat tcatagacgc
    12481 tgatgcctta tctatgaggc ttttcccatt gttgaataca ttttcagaag gtttatcaga
    12541 ccagagtgga tgtatacctg tcatggcctg tccccagtaa ccaattgcac actgcggatc
    12601 aaccgatgta gccgtctgaa actctttgtt tgcaagacca tatgtcatat gatgaagaag
    12661 agcaaggcca cggttaaggt gaaccgcagc tgccgggtcg catgaagtct gaaaatttac
    12721 agtcccaagc ttcgataaat cggttgttgc ctgctgtaca ggttttttat ctacttctgc
    12781 ctcagggggt gcggtatcct tggccgcgca tgaagcaaaa ataatcgcaa aagctaataa
    12841 taaaaattta tgcatattgt ccatctcctt gtgatttagt tcattttaaa atcccaaacc
    12901 tattttaata aagtttataa gaaaaataaa gataaattta tgttttagta actatttgga
    12961 attttaatca cttttaaatc ggcttattga aatattaaca agtttttcac taacccctgt
    13021 aatatgctca agatcgtctt tgctgagctt tgatgtgaaa tgttctttta ttgaatcata
    13081 gtacactggc cacatattgt ttcttagctt tttaccttta gcagtaatta ctgcaaacac
    13141 tcctcttgtg tcctctttac agggcatgcg ttttattagt ccttcttttt caagtcggtc
    13201 aaggagcctg gatacgttgt atttagaaag gagtataagc tctcccagtt ctttttgtct
    13261 cagccttcca ccttttgcct tttcaagctc aagcagaacg tcgtaccatg aaagaggagg
    13321 aaatccactt gccttaaaat ccctgtctac tttctctaat actgtatgct gagcctttac
    13381 aaaacctatc catgctcttg tttccatatt cgatagtttt ttattcataa aattatttta
    13441 acaaatatag ttgcatttgc aacattaatc cgtatattat atatagttgc agatgcaacc
    13501 attaaaatac ggaggtattt attatgattg aaagaattaa cagacaggga gttgttgatc
    13561 taatcggcaa acctgagagc gcggttttgg ttgaggcact gccggaaaaa tattataacg
    13621 acgcccacat tcccggtgcc gttcagatgg actacaccga agtcaccgat aaggcccctt
    13681 ctgtacttgg agacaaagac cgcaagatag tggtttactg tgctagctcg gaatgtgaga
    13741 attctacaaa ggccgcccgc acactgcagc agcttggata cgacagaata tatgtataca
    13801 aggaaggcaa gcaggactgg attgaagcag gtcttagggt tgaatctgct ggtgagtaaa
    13861 atcatcaaaa catataaata tataggagga ggaatataac tatgatcggc aaattaatgc
    13921 acacagacag taatgataca ggtagcctta ttgcaagggt agttgcagga gctgtaatgc
    13981 ttccccacgg actgcaaaaa ttgctcggga tgtttggagg ttatggcttt agcgccactc
    14041 ttgatttctt tgcatcaagc ggccttccaa aactcatcgg ttttttgata ataatgggtg
    14101 aatcagtagg tgctgtaatg ctaatcgttg gactattcag caggtttgca gcagctagtc
    14161 ttactataat aatgcttggc gcaataaaaa ttcatcttga aaacggattt tttatgaact
    14221 ggtttggaaa ccagaatggt gaagggtttg agtttcatat actcatggtc gggttacttt
    14281 taattgtagc cataaagggc tcgggaaagt ggtcagtaga ctctattctg actaaatagc
    14341 cagtatatat agccatgata ttacactagt tagtgtaacc cagatggatt tttgcagttt
    14401 atatatatat gactaggcca agacttatca tctctttttt attgctgggt ctgatattga
    14461 acccatttgc ggtttcttca gaagagaaga aatacaaggc atttaccgat atcggaccca
    14521 tagaaaaaag tgatttaacc atcactgcga tattttctga caaggacaaa tatcatagag
    14581 aggttctcac tgttgagggg actgtagcta agctcaaata caaaaaaatg tttaacggca
    14641 gaaagtttac atattttagc cttgctgata gtgagaataa tactataaac gtgtatgcaa
    14701 gaggatatgt aaaagatata aatgaaggct cggaaattag aatttacggc agatatagta
    14761 aagaaaagtc attcttgttt aaaaagcgaa agaatgttat gaaggcaaga aagatcgaga
    14821 tattagacca atctgaaagt gttgttttgc aaacagaaca aaaaactcag ccctaagatc
    14881 aacccagctg tattacagtt cagtaataat ttttactcta aaacaatcag agatcatgtc
    14941 ctgcaaactc ggcatcaaac attgcctgaa gctcggctgc tttctcgtca tacttcttgg
    15001 tgcttttcca tgtaagcctt ggctgtagag tttctttttt tacaccggga caccttgttg
    15061 gtacattgag cccgaatatc ggaacctcgg taaacttagc cttactgagt gatccatcaa
    15121 gcgcagcagt aacaagagcc cttgtttcag gaagcggcat acgttttccg actccatatg
    15181 ggccgcctgt aataccggtg ttgagaagcc acacatttgc tctgtttttt ttaagttttt
    15241 tgtgaagcat gtctgcatag aactttggat gacgcggcag aaacggagcg ccaaagcatg
    15301 aactgaatgt cacctgaggt tctgttaccc ctctttcagt accggctact tttgccgtgt
    15361 agccgatgat aaagaaatat atagcctgct tgagtgttag tttagatatg ggtggtagca
    15421 cgccaaacgc atcatatgta agcatgaaga tggtctttgg aacaccgcct actccctctg
    15481 ggacaatgca atcaagatga tgaatcggat aggcaaccct tgtgttctct gtatacttta
    15541 tgctgtcaaa atcaattctt tttgtatcgg gatcaaattc tacgttttca accacagagc
    15601 cgaactttaa tgatgcatta tagatttcag gctctgtctt ggggctgagc ttgatggctt
    15661 tagcatagca tccgccctca aaattaaata cgcctttatc aaaccagccg tgctcatcat
    15721 caccaataag tggtcttttg ggatcggcag agagagttgt ttttcctgta cccgagaggc
    15781 caaagaaaag tgatacatcc cctttctgtc ccacatttgc cgagcagtgc atcgggaata
    15841 cattttgttc aggaagcaga taatttagcg cactgaatac cgattttttc atctcgcctg
    15901 catattcggt tccgcctata agagcgattc tttttgaaag attcagtatt ataaaagtct
    15961 ctgtacgagt gccgtccctt ttgggattag ctttaaagct tggagaacta attacggtaa
    16021 attcgacttt cttatgtgaa tatttgtcat catcaggtct taaaaacaag ctgtttacaa
    16081 aaatattgtg ccatgcatat tcgttaatta cccttacagg catcttgtaa tcatcatgag
    16141 cgcaggcaaa aaggtctctt acaaagaggt ctttaccatt tagatgtttt agtaccctgt
    16201 ttagcagtgc atcaaatttc ctggaatcaa aaggcttgtt aacctttccc cagtttattg
    16261 tgttcttgtt ctttgagtcc tgcacaataa atctgtcctg cggggagcgc cctgtgtgtt
    16321 ttcctgtgtg ggcaacaata gtgccgcctt cacctatagt gccttcgttt ctctttatga
    16381 attcttcata taaagtggga ataggaaggt tatagtaaat atttcctggc tttgttatac
    16441 cgtgtttctt tgctactcgt ttgccaacct ctgaattaga cattcagtcc tccgtttttt
    16501 ttgtcaataa tggaaatcta tggagagtgc tagatttaca atttcaaatt ttaaaaaagg
    16561 aagaatttaa caaatctcaa caaaaattca acatgcagat aacattaatt ttaaataact
    16621 tcatattatc tactttattt tcttcacaaa acctgaaatt gccgagattg tggcctttgg
    16681 attttccata caatcgtgct ttgcgtcggg aataaatctt aatttaacat tactgtttcc
    16741 agctctttcc agaatctttt taaggtcttt tggctcccat gcaaaaacaa tatcgtcatt
    16801 tttgccgttt ataaaaagta ccggaacctt aatcttatct ataagtttat aggtctttgc
    16861 attctcggct tgcgggcttc tcataaacca ccaggtctta taagaaaaca tctcacaatc
    16921 aattggatta aaagtaggcc cccacgccct gtatactacg aatatcctgt catttatgga
    16981 ctttagcgga tttggagaca gtagatgctc agccttttca tatatatcct catagcctgg
    17041 aatactgttc cacttttgca gtctatcctt ttgagattcg ggaagagaat atgaacagcc
    17101 ctcgaggatt actcctttta cgtttctgtg tgtatttacc gaagcatagt gggcagtgag
    17161 atttgcgccc aggctgtatc cgagaataaa tattctgtga aatccaatct taacaagata
    17221 tctcacagct gcatctatat cataaatggt ttcatcaaaa attgcgctat caataatttg
    17281 gcccatatga gccattcgtg agttaatcga aagtgaatgt ataccaacat caaaaagcgc
    17341 cggtggaagc agtctcggag tgcctcttgt aagaaagttt ccaagaacac catgaacctg
    17401 aataattatg ggagtgttat atagctcttt ttcatttctt tttttacata tggtaagtac
    17461 ggcattaacc tcaaatccat ctttggcttt aaaattaaca agctttttat gagagatatt
    17521 catatgggga tttaattcta tgagataagt ctattcttca aatttagagt taagccatcc
    17581 aaccgcgata tctcccagtt ctttttcatt accctcgaaa gcatgaccac tgtctacaaa
    17641 aaacaccgat acactcttgt ttcctgcatc tcttgctacc tgagccagat gataggtttc
    17701 ctgcgaacta acaacttcat cgtaccagcc ctgaacaagt aaaataggaa gctttatctt
    17761 ctctatctga ttataacact taccactctc ggcatgggga ctagcaagat gccaccatgt
    17821 tttgtatgta taaattccac tgtgttccgg tatataagag tctcctctgg tcttatatat
    17881 cacaattgtc ctgtcttcat cggtattatc gggatcgccc ttgaagattg cttccgcttc
    17941 tttataaatc tggtcatatg acggctcgct tccccattta tcccatcttc tttttataga
    18001 atcgggcatc gaatacggag ctgccaggac tatgactcct tttatatgag gatacttctt
    18061 ttctgaattt ctcatggcca tgtacctgag cgcaacggca ccgccaaggc tgtaacctga
    18121 taccacaata ttcttaaatc cctgttctaa taaaaactct attgatctgt ctatttgggg
    18181 gatagtatcg tctatgattc cgtagccaaa aaacaggccg aaatttgcaa gcctggtatt
    18241 aattgaaagg gatgaatagc cggactttgc caatattgga ggcagaaatc tctgcgtacc
    18301 atctaaaaaa ttgcccagaa atccatgaac atgaattacc gctgtttttg atatttcttc
    18361 tgatacattt ttcttgcttt tataatatag agcgtcaacc ttgctacttc caaagggaat
    18421 agctatgagc tcctgttcca tatatttcat agttagagaa taatcctttc aatgcaaaat
    18481 tggtctgctt aaacaaatca accatggacc taaaatacag aataaccaat tttatagatt
    18541 taagaaggaa aaaatttgga ggggtcgctt aaaacttcat cggcaaattt tttagactca
    18601 aaccagtcat ttatatcagc aagctgtata attttcttgg actgcgcccc ttttgcatag
    18661 aatttatggt ttatagaatc
//
LOCUS       DBNGSV010000032         1231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_281209, whole genome shotgun sequence.
ACCESSION   DBNGSV010000032 DBNGSV010000000
VERSION     DBNGSV010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_281209"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="AC159U_01305"
     CDS             <1..1053
                     /locus_tag="AC159U_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335727.1"
                     /translation="TIVPTPLAKKTITDKLAELEKIGLKRNDPIMSTLEDWEQALEHK
                     DIATIEKIRSDIFESFTGAEGNIRKRGQKAVDDVYSALRDDMDNYIKLKGEPGDFNKF
                     NDAMSELSNLSKDYNTEGISTLMENRKAPTKTIENIILNGKPDEIELIYRRLPPEGQS
                     NARAAMISSFIEKATSGGQLTARGVEADISPNKIAANIRKYSGQHGVLFSAEDSEHLS
                     SIGKIFESTSRAEKDALIREIFPGESVPGVKYGIARLISQNPVVGIPAALSWGPFTGA
                     MARFMEKPAMRNLMLKYDDLPTRARSGLQGDELLKRMGEVIKGYLETEEVEESDAAME
                     RISGAYSQENTLEGRK"
BASE COUNT          445 a          184 c          269 g          333 t
ORIGIN      
        1 accatagttc caactccttt agctaaaaaa actataactg ataagttagc agaacttgaa
       61 aaaataggtt taaaaagaaa tgatccaatt atgagtactt tagaagattg ggaacaggca
      121 ttagaacata aggatatagc tactattgaa aagatacgtt cagatatatt tgagtccttt
      181 acaggtgcag aaggaaacat aaggaaacgt ggtcaaaaag ctgtagatga tgtgtatagt
      241 gctcttagag atgacatgga caactatata aaacttaaag gggagccagg agactttaat
      301 aagtttaatg atgcaatgtc tgaactaagt aatttaagta aagactataa tacagaaggt
      361 atttctacac taatggaaaa tagaaaagca cccactaaaa caattgaaaa tattattcta
      421 aacggtaagc ctgatgaaat agagcttata tatagaagat tacctcctga aggtcaaagc
      481 aatgctcgtg ctgccatgat ctctagcttt atagaaaaag ctacttcagg tgggcaactt
      541 actgctagag gtgtggaagc agacatatct cctaataaaa tagcagcaaa tataagaaag
      601 tacagtgggc aacatggtgt acttttcagt gctgaagata gtgaacatct atcttctatt
      661 ggaaagattt ttgagagtac aagtagagct gaaaaagatg cccttattag agaaatattt
      721 ccaggtgaaa gtgtaccagg tgtaaaatat ggaattgctc gtcttatatc tcagaaccct
      781 gttgttggta ttccggcagc attaagttgg ggtccattta caggagctat ggctcgattt
      841 atggaaaaac ctgcaatgag aaatctgatg cttaaatatg atgatttacc cacaagagct
      901 agaagcggtc ttcaaggaga tgagcttctt aaaagaatgg gggaagttat caaaggatat
      961 cttgaaacgg aagaagttga agagtctgat gcagcaatgg aaagaataag tggagcatat
     1021 tcacaagaaa acactttaga gggtaggaaa taatggcaga actagtacag aacccatatc
     1081 cggtgttcac tgatatagat ggaacttcat tagaagatgg atttatcttt attggtgttg
     1141 cgggacttga tccaattaca aatcctcaac aggcttattg ggatgaggct ctcactatcc
     1201 ctgctaacaa tatcaggact cgtggtggtt a
//
LOCUS       DBNGSV010000033         4434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_696761, whole genome shotgun sequence.
ACCESSION   DBNGSV010000033 DBNGSV010000000
VERSION     DBNGSV010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_696761"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..96)
                     /locus_tag="AC159U_01310"
     tRNA            complement(23..96)
                     /locus_tag="AC159U_01310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:Arg,seq:acg)
     gene            complement(126..214)
                     /locus_tag="AC159U_01315"
     tRNA            complement(126..214)
                     /locus_tag="AC159U_01315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Ser,seq:gct)
     gene            complement(279..1241)
                     /locus_tag="AC159U_01320"
     CDS             complement(279..1241)
                     /locus_tag="AC159U_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335728.1"
                     /translation="MMISLTGLFTVLFSLLISSYAFSDDLNIEIIKKGSLSKDIIVDS
                     VVANDKESLSELWKQMQDKSQIPTVNFDKESVFFIISKNVKAEKIEITKVEAKPGNKV
                     NIIYTISESNGKSINSRLSGTDYPYIIGRIENPNNPKYEISLRENYKVPPIPANQALE
                     QDIKYTNTLSQYNLEIVNYVPLDLGNKWTYKIESDKRNAEVTQEVQSVSNGWSVLNSY
                     FGTQNIAMKIEHSGQLIVSSDAGVRAFYTNDVQTQFVKEEIKTPAGSFKDLMIVTIPQ
                     NNNFWFRDVYAKGVGLVLHEHISPKGNGKYTLVKAKVRGKDYPG"
     gene            complement(1300..2565)
                     /locus_tag="AC159U_01325"
     CDS             complement(1300..2565)
                     /locus_tag="AC159U_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335729.1"
                     /translation="MTKPVLSAYIDTSEFADLIDLELRAPLYQILSIVDSLYSTQITG
                     RNLHETENAKYKEIINLLYKCADYLSSESASKLREQVQRIENGIYDLAVIDISTSDGI
                     TVVLVFGKVISSTDSKKYYSAALGTEDTRFYSLIADYKKFISENTQKFELVEKSELNR
                     LTNLNNSFKCVNAIKFAGYINKDHLPFSLFYSGGKKQHLSSLTNAVLFTNLYFERYKL
                     ISQKLADLIIDDDGNTENPEPSMSEKILTLWLWGHDTGHFLSNDNLRDNVESDNEYLY
                     EVLHELRSDIFSLYLLKYTCEQLLLIDQGKVYSVFVAEMLRYIRRGEFINQPDSVSAF
                     VVFKYMIYKHALVIEEGSNEIVLNFAKFGKLIDELMSFCINIFATGDFLLADDLLSIL
                     KMKRDFSNLDDQLLLDLTFTDIAVNIKIV"
     gene            complement(2606..4162)
                     /locus_tag="AC159U_01330"
     CDS             complement(2606..4162)
                     /locus_tag="AC159U_01330"
                     /inference="COORDINATES: protein motif:HMM:NF025499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HIR3335730.1"
                     /translation="MFELQDRDKEHLIKKGITEKKFYDQLKLLKQGVPFIDLVRPCTM
                     ENGVKSLGEIELEQCIDLYENSLGRVNPVKFVPASGAASRMFAVLSKLLNNYEQYEDK
                     ISGDSKDNDTNSLLQFTDNINSFAFAEDLKDVIKNNGGDLQGLIDDKNYKVILEHLLT
                     DKGLDYLNCPKGLIKFHKYEDQVRTAFEEHLFETKSYSKNSGGTAWVHFTILKEHEHK
                     FNEYARSVISKYEGDGVKLNVSFSFQSPDTDIVAVDFDDNPIRNKDGELVFRPGGHGA
                     LLQNLGDLDAEIIFVKNIDNVVPDRLKDKTIIYKKALAGYLLDLKKNIDDYLNLISEN
                     KINDSQLEDARGFINDRLEIEIPADFEAKSAEQKKEYIFNVLNRPLRVCGVVKNEGEP
                     GGGPFWVKDLSGNISKQIVESAQVDINNKEQSRIWNSSTHFNPVDLVCSIYDCKGNKF
                     DLDKFVDMNTVFITKKSKDGTDLKAMELPGLWNGSMAYWNTIFVEVPLITFNPVKTVF
                     DLLKPEHQAG"
     gene            4273..4344
                     /locus_tag="AC159U_01335"
     tRNA            4273..4344
                     /locus_tag="AC159U_01335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4305..4307,aa:Thr,seq:cgt)
BASE COUNT         1326 a          894 c          710 g         1504 t
ORIGIN      
        1 attaattaaa tatataaaga accgcgcccg gagggagtcg aacccccaac cttgtgatcc
       61 gtagtcacac gctctgtcca gttgagctac gggcgcaaaa cttaatttta attaaaatag
      121 aatttcggag agggtgggat ttgaacccac gagggggtat aaacccccta ctcgcttagc
      181 aggcgagcgc cttcggccac tcggccacct ctccatcact tttaaaagga cataaaataa
      241 ctgctagtat tataaaaagt caaacaaata atgccagatt atcccggata atccttacct
      301 cttacttttg ctttaacaag cgtatacttt ccatttcctt ttggcgagat atgttcgtgc
      361 agcaccaggc ccactccctt tgcataaaca tccctgaacc aaaaattatt gttttgaggt
      421 attgtaacaa tcataagatc cttaaaacta cctgcgggag tcttaatctc ttctttgaca
      481 aactgggtct gaacatcatt tgtataaaac gccctaacac ccgcatcgga tgagacaatg
      541 agctgtccgc tgtgttcgat tttcattgca atattttgtg tgccgaaata gctgtttaat
      601 actgaccagc cgtttgatac agactgaact tcctgtgtta cttccgcatt cctcttatca
      661 gactctattt tatatgtcca cttattgcca aggtccaaag gcacatagtt tacaatctca
      721 agattatatt gagatagagt gttggtgtac tttatatcct gctccaatgc ctggtttgcc
      781 gggattggag gaaccttata attttctcta agacttattt cgtactttgg attattaggg
      841 ttttcaattc tgccaattat ataaggataa tccgtaccgc ttagtctgct gttaattgat
      901 ttaccgttgg actcactgat tgtataaatt atattcactt tatttccagg ttttgcctct
      961 accttggtaa tctctatttt ctctgccttt acattttttg atattatgaa aaagacagat
     1021 tctttatcaa agttaacagt agggatttga gatttatcct gcatctgttt ccataactca
     1081 gaaagactct ctttatcatt tgcgactaca gaatctacta taatatcttt actaagcgat
     1141 ccttttttaa ttatctcaat atttaaatca tcactaaatg catatgatga aattaacaac
     1201 gagaacaata ctgtaaacag acctgttaat gaaatcatca tcttcatact tccttacctc
     1261 ccaaatgttc ataaaaatta tacaatcttt taagctcaat taaacaattt taatatttac
     1321 tgcaatatct gtgaatgtta gatcaagtag aagttgatca tctaaattgc taaaatctct
     1381 tttcatttta agtatactca ataaatcatc tgccagtaaa aaatctccgg tggcaaaaat
     1441 attaatgcaa aaactcatta actcgtcaat taatttacca aattttgcaa aattcaaaac
     1501 tatttcatta gacccctctt caattactaa agcgtgttta taaatcatat atttaaaaac
     1561 aacaaaggca gataccgaat caggctggtt tataaactcc cctcttctta tgtaccttag
     1621 catttcagca acaaacacac tgtagacctt tccttgatca attaatagta attgctcaca
     1681 tgtatatttg agcaggtata acgaaaatat atcggagcgc agctcatgaa gtacttcata
     1741 aagatactca ttatccgatt cgacattatc tctcaggtta tcatttgaga ggaaatgacc
     1801 tgtatcatgt ccccataacc ataatgtcag tatcttttcc gacattgagg gctctgggtt
     1861 ttcggtatta ccatcatcgt caattataag atctgcaagt ttttgtgata taagtttata
     1921 tctctcaaaa taaaggttcg taaatagtac tgcatttgta agtgatgaaa gatgctgttt
     1981 tttaccaccc gaataaaaga gcgaaaaagg aagatggtct ttatttatat atcctgcaaa
     2041 ttttatagca tttacgcatt taaagctatt gttcagattc gtcagtctat ttaattctga
     2101 tttttctaca agctcaaact tttgtgtatt ttctgatata aactttttat aatcagcaat
     2161 aaggctataa aatcttgtat cttcagttcc aagcgctgca ctgtagtatt tctttgaatc
     2221 tgttgaggat attactttac caaaaacaag aacaacagta atgccgtcag aagtgcttat
     2281 atcaataaca gcaagatcat aaataccatt ttctattctc tgcacctgct cccttagttt
     2341 agatgcactc tcggatgata aataatcggc gcatttatat aaaaggttaa ttatctcttt
     2401 atattttgca ttctcagttt catgcaggtt ccgtcctgtt atttgagtac tatataagga
     2461 atcaactatg cttagaatct gatagagcgg tgctcttaat tccaaatcta ttaagtcggc
     2521 aaactccgat gtatctatat aggcactaag aaccggttta gtcatttata taggttagca
     2581 ggcttatcaa tctaaaagat agaatctacc ccgcctgatg ttctggtttt agaaggtcaa
     2641 acactgtttt tactggatta aaggtaataa gaggaacttc tacgaatatg gtattccagt
     2701 atgccataga tccgttccaa agtccgggaa gctccattgc ttttaggtcg gtaccgtcct
     2761 ttgatttttt ggtaataaac actgtgttca tatctacaaa cttatcaaga tcaaatttat
     2821 ttcccttgca gtcatatatt gagcaaacaa gatcaacagg attaaaatgt gttgatgagt
     2881 tccatatacg gctttgctct ttattattaa tatcaacctg cgccgattct acgatttgct
     2941 tactgatatt tccagaaagg tctttaaccc aaaatggccc gccgccgggc tccccttcat
     3001 ttttaacgac tccacaaact ctaagtggtc tgttgaggac attgaatata tattcctttt
     3061 tttgttcagc tgatttagct tcaaaatctg ctgggatttc tatttccagt ctatcgttta
     3121 taaacccacg cgcatcttct aattgagaat catttatctt attttcagag atgaggttta
     3181 aatagtcatc tatattcttt tttagatcaa gcaagtaacc tgcaagagcc ttcttgtata
     3241 tgatagtctt atctttgagt ctgtcaggga cgacattatc aatatttttt acaaatatga
     3301 tctcggcatc caaatcaccc aggttttgaa gcagtgcccc gtgaccacct ggcctgaaca
     3361 caagctcacc atccttattc cttataggat tgtcatcaaa atcgactgcc actatatcag
     3421 tgtcaggact ctggaaggaa aatgaaacat tcaattttac tccatcgcct tcatatttac
     3481 ttattacaga cctggcatat tcattaaatt tatgctcgtg ttccttaagt atggtgaaat
     3541 gcacccatgc tgtaccacca ctattcttag aataactttt tgtttcaaaa agatgttctt
     3601 caaacgcggt tcttacctga tcttcatact tgtgaaattt tataagcccc ttgggacagt
     3661 taagatagtc aagccccttg tctgtgagca ggtgttctaa gataacctta taatttttat
     3721 catcaattaa cccctgcaag tccccaccgt tgtttttaat tacatcttta aggtcttcag
     3781 caaaggcaaa gctgtttata ttgtcggtga attgcaacaa tgaattagta tcgttgtctt
     3841 tagagtcacc cgaaatctta tcctcatatt gctcataatt gttcaacaat ttgctaagca
     3901 cagcaaacat tctactggca gcacctgatg cgggaacgaa ttttacaggg ttaaccctgc
     3961 caagactatt ttcatataga tctatgcact gctctaattc aatttcacca agggatttaa
     4021 ctccattttc catggtacag ggccgtacaa ggtcaataaa tggaacaccc tgtttcagaa
     4081 gctttaactg atcataaaac ttcttctctg taataccttt ttttataaga tgttctttgt
     4141 cacggtcttg cagttcaaac atagaaaagg attataaatt gtgagaacag attatcaaaa
     4201 atttagagaa aatcggccta tggaaggttt gattgtaaaa ttttggatgc tataatctgg
     4261 ttttaaattc ctgccgacgt agctcagtgg tagagcagct cactcgtaat gagcaggtcg
     4321 ggagttcaaa tctccccgtc ggcttaataa ttacaggcgg ttacaacgcc ttcaatattc
     4381 tctcaaattt attgctagag tttttgctct catttttgaa ctctcagact cttt
//
LOCUS       DBNGSV010000034         6982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_350907, whole genome shotgun sequence.
ACCESSION   DBNGSV010000034 DBNGSV010000000
VERSION     DBNGSV010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_350907"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="AC159U_01340"
     CDS             complement(<1..1432)
                     /locus_tag="AC159U_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3335731.1"
                     /translation="MKHSFNNLNVASFESRRSKEIGRLIEYHGGTAHIAQSVVEVPSK
                     SSKETEELCIKLKNGDIDIIIFLTGFGTKLLLENLGKRFGTKPAKKLLNNITVVGRGI
                     KSRSVLRNLGIKPLYSKPIPNTWREIVKVLADKDLLENKNIAIQEYSKKNSELIRALK
                     KSGANVLSIPVYKSELPKDTEDLENLVNNIIKGNIDICLFTSSQQVANIFRFAKKLKL
                     NEQLRQGFKKVVIGSVGTNTSLALTDHGIKADYEPENSRMGNLVREIARRSEYLLTKK
                     RASAKNRVDTNKWERIDTNWGKISDKNRRAITNNSQFMKACRRKKTDYTPIWIMRQAG
                     RFSRHYRAIRSNHTFLDLCKTPEVSSEVTLMAVDQLGVDAAIIFSDILLILEPLGINI
                     TYTKSDGPKILNPLRTKKNIERIREFNTDELDFMYESLRITRRALNPQIPLIGFAGAP
                     FTLASYAIEGGSSRNFELTKSLMYKDP"
     gene            1860..3107
                     /gene="lysA"
                     /locus_tag="AC159U_01345"
     CDS             1860..3107
                     /gene="lysA"
                     /locus_tag="AC159U_01345"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3335732.1"
                     /translation="MFSYKNGEFCAEDVPVNKIAQEVGTPCYVYSYKFIEDTFNDYKD
                     AFMHIEPIICYSAKANTNLSILKAFSSLGSGFDIVSVGELKKALKIGADPNKIVFSGV
                     GKTREEIVAAIEAGILFFNVESVDELEFINEIAISLGKKAPISLRVNPDINPKTHPYI
                     STGFKKSKFGIEINSAFEVYKDSVNKEGLEIIGIDAHIGSQIFEITPFIDSLRKLIVL
                     ADRLVAEGINIQYIDIGGGLGIKYENEEQPLSKKDFADAIIKELSGKSYKIVLEPGRS
                     LIGNGGILLTSVLYNKVGTEKKFIIIDAAMNDLIRPAFYNSYHEIVTATENNSTKNIY
                     DIVGPICESGDFLAKDREFPQVCKGDVLAVKSAGAYGFVMSFNYNSRPRAAEVLVRGD
                     KYFVIKQRESFEDLIKGEEIINF"
     gene            3171..3824
                     /locus_tag="AC159U_01350"
     CDS             3171..3824
                     /locus_tag="AC159U_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3335733.1"
                     /translation="MSELNSEIIEIIRKAGIFVYPIFLCSIVALSIFLQKMWTLRTRK
                     ILPDTFLERLYTYLSKHKHTEALNLASLNNSSIARIAYAALQKSGAPKVEIRESMEEA
                     GKREAIELGRYTEGLATISNVSTLLGLLGTIAGIIKVFSVISEQTVVDPPSLAGGISE
                     ALYTTAFGLVVAIPAFIAYKYLLGRSEEFVNNLEEEGRRIIEEMTVNKQKNSEDDLL"
     gene            3821..4264
                     /locus_tag="AC159U_01355"
     CDS             3821..4264
                     /locus_tag="AC159U_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3335734.1"
                     /translation="MNFRTRTNNGKKGVGATLDLIPLVDVVFNLLIFFALSLNFAMAS
                     KGIDVKLPSASSAKTIKSDQVTVNLTKQGEIFLNDKKTTTGNLGAQLKEKADKKALII
                     IRADNDVEHGRVVEVMDIIKSNDLNRIAIAVDAGAPAASDEGESN"
     gene            4286..5212
                     /locus_tag="AC159U_01360"
     CDS             4286..5212
                     /locus_tag="AC159U_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013999009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIR3335735.1"
                     /translation="MNTLSSINRILTRYFTLWIILFSVYSYYFPYELSKLTFLIKPAL
                     IVIMFGMGMTLTPGDFKEVLRRPKEILLGSSLQYFVMPLLGFLLAKIFGLSPELAAGV
                     ILLGSCPGGTASNVIAYLAKGDVALSVSLTSVSTLLSPLLTPIMTYIYADKFIDVPVY
                     GLFLSALQIVLAPVAMGVIIRSLVKDKIDTVIEFLPSISALAIIFIVGVIVAVSKDSI
                     AKLGFSLGIIIFLHNSLGLTIGYTAARLLGMDSKKARTISIEVGMQNSGLGVVLAKTH
                     FSLLAALPSALFSVWHNVSGSLLALLWQRKSN"
     gene            5234..6127
                     /locus_tag="AC159U_01365"
     CDS             5234..6127
                     /locus_tag="AC159U_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3335736.1"
                     /translation="MKSKTKKLPITGYLFLLSTAFFTAISYVCGKAVNKDLGPDVVTF
                     YWFFGAFLFSLFVLPLFKAQRNEFKNIGKYKMIFLLSSIVTSIGAALWIFSLRSIGLP
                     LTSFLMKAQTLFTLFLGIIFLKEKLNKGETIGIIITIIGGVIVAYQREGYLILGTATA
                     LTAAFFYSVLSFAVKKYASDLNMLTVANLRTMGVSIVVFIYLLILGKFELPSGIDFIY
                     MALGGLTGAYIAKASQFQSIKLLGIARSTAVMPLESIFVIIFSYTIFNELPSAIKLAG
                     GFIIVFGVYFLVIYRGGEQKN"
     gene            complement(6124..>6982)
                     /locus_tag="AC159U_01370"
     CDS             complement(6124..>6982)
                     /locus_tag="AC159U_01370"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3335737.1"
                     /translation="PKQKTKKISIDCAFPILHGTYGEDGAIQGLFEIMNVPYVGANVH
                     GSAIGMDKISMKSVLKDYGIPIVDFTAFDIGTWNKNSNSILRKIKAEIGFPCFVKAAN
                     LGSSIGITKVDSSKDLKKSIEYSLKFSDRILVEKAVKDPREIEVSVLGNQDPKASVAG
                     EIVPKRGFYDYEAKYSDDATELIAPAKLSKQITKKLQDIAVAAYKALDCSGLGRIDFL
                     MESESQRIFVSEINTIPGFTQISMYPKLWELSGIKYKKLVTTLIELAMDKHEEKNKLN
                     TDYEQKLKV"
BASE COUNT         2067 a         1224 c         1331 g         2360 t
ORIGIN      
        1 ccgggtcttt atacataagg ctctttgtaa gctcaaagtt tcgggacgat ccgccttcaa
       61 ttgcatagga agcaagagta aatggtgcgc cagcaaatcc tataagtggt atttggggat
      121 ttagagccct gcgagtgatt ctaagggact catacataaa atctaattcg tctgtattga
      181 actctcttat tctttctata ttttttttgg ttcttagtgg atttaatatt tttggcccgt
      241 ctgattttgt ataagtaata tttattccaa gcggctctaa aattaataat atgtctgaaa
      301 aaattattgc agcatcaacc cctagctgat caacggccat tagtgtgact tcggatgaaa
      361 cttctggagt tttgcaaaga tctaagaatg tatggttaga tcttattgct ctatagtgtc
      421 ttgagaacct tccagcctgt ctcatgatcc atataggagt gtaatctgtt ttttttcttc
      481 tgcatgcctt cataaattga gaattatttg ttattgctct tctattttta tcagaaatct
      541 ttccccagtt ggtatctatc ctttcccatt tgttagtatc gaccctgttt tttgcagaag
      601 ctctcttttt agttagtaag tattccgacc ttctggcgat ctctctcaca aggttgccca
      661 tgcgtgagtt ttctggttca taatcggcct taatgccatg gtcagtaagt gcaagggaag
      721 tatttgtccc aactgaacct attacaactt ttttaaatcc ctgcctgagt tgctcattca
      781 gttttagttt ttttgcaaat ctgaatatgt ttgcaacctg ctgggagctt gtgaaaagac
      841 atatatcgat attccctttg attatattat ttacaagatt ttccagatcc tctgtatcct
      901 ttggaagctc agatttatat accgggatac ttaggacatt tgccccactt ttctttagag
      961 ctctaattaa ttcactattc tttttagaat attcctgaat agctatattt ttgttttcaa
     1021 gtaaatcttt atctgcaagt acctttacta tttcacgcca tgtgttgggt atgggttttg
     1081 aatagagtgg cttgattcct aaattcctta aaaccgatct ggatttaata cccctgccca
     1141 ctactgtaat attatttaag agtttttttg ctggttttgt accaaacctc ttcccaaggt
     1201 tttccaaaag aagttttgtg ccaaatcctg taagaaaaat gatgatatca atatccccat
     1261 tttttagttt tatgcacagc tcttcagtct cctttgaact tttggatggc acttctacca
     1321 cagattgagc aatatgtgca gtaccaccgt ggtattctat cagcctgcca atttcttttg
     1381 atcttcggct ctcaaatgaa gctacattta gattgttaaa actatgtttc atattgaaat
     1441 tataaatcag ttagagataa agtctgagtt tagcacacga ctttttaagt tcaatctcga
     1501 gattaatcta aacacattat tctttcatat taatgatacg gcatgcctta ttgtcatata
     1561 cataatgtgt aataattttt ctataaatat atagtaagga gaaaataatg aaatattaaa
     1621 tattgtgatt tatgaagaat ttattaaata acctctcaag gctactttaa aatgttataa
     1681 gaatttttga tattgtgcta aaagagattg caagaggaga atctataaaa atgatattag
     1741 aattaaggga atattgatgt tgcattggta gtaaactatt tgagtttgta atttccgatt
     1801 tataagagat attattaggt caatttttga gattataata gtgctagagg taattattga
     1861 tgttttctta taagaatggt gaattttgtg ccgaagatgt gccagtaaac aagatcgctc
     1921 aagaggtggg aaccccgtgc tatgtgtaca gctataaatt cattgaagat acgtttaacg
     1981 attacaaaga tgcatttatg catattgagc caattatctg ctattcagca aaagcaaata
     2041 ctaatctttc aatcctaaag gctttctcct cgcttggttc cggatttgat atagtttcag
     2101 taggtgagct aaaaaaagcc ctcaaaatcg gagctgatcc aaacaaaata gtcttttcag
     2161 gagtaggaaa aacaagggaa gaaatagttg ctgctattga ggccgggatt ttgtttttta
     2221 atgtagaatc agttgatgaa ctggaattta taaatgagat tgcaatatca cttggtaaaa
     2281 aggctccgat ttccctaagg gttaatcctg atattaatcc aaagacacat ccttacattt
     2341 ctacaggctt taagaagagt aagttcggga ttgagattaa tagcgccttt gaggtttata
     2401 aggattctgt aaacaaagaa gggctcgaaa taatagggat tgatgcccat atcggatcac
     2461 agatttttga aattacaccg tttattgatt ctctcagaaa gcttattgtt cttgcagaca
     2521 gacttgtagc agagggcatt aacatacagt acatagacat tggcggaggt cttggaataa
     2581 aatatgaaaa tgaagaacag cctctatcca aaaaagactt cgcagatgca atcattaaag
     2641 agctttcggg caaatcatat aaaatagtac tcgaaccagg aaggtcgcta attggaaacg
     2701 gtggtattct ccttacctcg gttctatata ataaagttgg tacagagaaa aagtttataa
     2761 taattgatgc agcaatgaat gatcttatca ggcctgcatt ttacaactct tatcatgaaa
     2821 ttgttacggc tactgaaaac aattctacta aaaacatata tgatatcgtc ggcccgattt
     2881 gcgagtctgg agattttctt gctaaagaca gagaatttcc tcaggtttgt aaaggcgatg
     2941 tgctcgcagt taaaagcgca ggtgcttatg gttttgtgat gtcatttaat tataattcca
     3001 gaccgagagc cgcagaagta ttggttaggg gtgataaata ttttgttata aaacaacgtg
     3061 aatcttttga agatttaata aaaggtgagg aaatcataaa tttttgatta tacttatcag
     3121 aatataaaaa ctttgaatat aatattcgaa ttaatccgtc ttaactactt atgagtgaac
     3181 taaattctga aataattgaa ataataagaa aagccggcat ttttgtgtat cccatattcc
     3241 tttgttccat tgttgcgctt tccatatttc tgcaaaaaat gtggacacta cgtacaagaa
     3301 aaatactacc ggatactttt cttgaaagac tttatacata cttatccaaa cataaacata
     3361 ccgaagctct taaccttgca agcctcaaca attcttcaat tgccagaatt gcctatgcag
     3421 cattacaaaa aagtggtgca ccgaaagtag aaataagaga atccatggaa gaagccggca
     3481 agagagaagc aattgagctt ggtcgttaca ccgaggggct tgcaactata agtaatgtaa
     3541 gcactcttct cgggcttctt ggaactatcg ctggtatcat taaggtattc agtgttatat
     3601 ctgaacaaac ggtagtagac ccgccgagtc tagctggagg aatctctgag gctctttata
     3661 caactgcgtt tgggcttgtg gttgcaattc cagcttttat tgcttataaa tacctcttag
     3721 gtagatcaga agaatttgta aacaatctcg aagaagaggg cagaagaata atcgaagaga
     3781 tgaccgttaa taagcaaaag aattcagaag atgatctgtt atgaatttta gaacaagaac
     3841 aaataacggg aagaaaggag ttggagctac tcttgatcta atacccctgg tagatgtggt
     3901 gttcaatctt ttgatattct ttgcgctttc tcttaacttt gcaatggcct cgaaaggaat
     3961 tgatgtaaag ttgccttctg caagttctgc aaagacaata aaatcagatc aggtcactgt
     4021 taaccttaca aaacagggtg aaatattcct caatgacaaa aaaacaacta ccggcaatct
     4081 gggagcacaa ttaaaagaaa aagctgataa aaaagctctt attattataa gggcggataa
     4141 cgatgtagag catggaagag tagttgaagt gatggatata attaaatcta atgatctaaa
     4201 cagaattgcc attgctgtcg atgccggagc tccggcagca tcggatgaag gtgagagcaa
     4261 ctaggcactt cattgattta ataaattgaa taccctaagt agtattaatc gaattcttac
     4321 aagatatttt actctctgga ttatactttt ttctgtatat tcatactact ttccctacga
     4381 gctatcaaag cttacctttt taataaaacc agcgcttata gtgataatgt ttgggatggg
     4441 aatgactttg actccaggcg attttaagga agtgctaaga aggccaaaag aaatattgct
     4501 tggtagttct ctacagtatt tcgtaatgcc tcttctcgga tttttactcg caaaaatatt
     4561 tggtctaagc cctgagcttg cagcaggggt aatcctgttg ggttcgtgcc cgggcggaac
     4621 tgcttcaaat gttatagcat atcttgcgaa aggagatgtc gcactttcag taagtcttac
     4681 gtctgtttct actcttctgt ctccattgtt aactcctata atgacatata tttatgcgga
     4741 taagtttatt gatgtaccag tttatggatt atttctttcg gctttacaaa ttgttttggc
     4801 gcccgtcgcc atgggggtaa taattagaag tctggttaaa gataagattg atacagtaat
     4861 tgaatttctc ccttctatat cagctcttgc aattatcttt atagttggag ttattgtagc
     4921 agtttctaag gattcaattg ctaaacttgg gttttcacta ggaataatta tatttttaca
     4981 caattcactt ggacttacta tcggttatac tgcggctagg cttttgggta tggactccaa
     5041 aaaggcaagg acaatatcca tcgaggtcgg tatgcagaat tcaggccttg gagtggtgct
     5101 tgcaaaaacc cacttcagcc tgcttgcagc actgccatct gcgcttttta gtgtttggca
     5161 taatgtgtct ggttctttgc ttgctttatt atggcaaagg aaatctaatt gaagttattt
     5221 ataaagatta atattgaaaa gcaagactaa aaaactccca ataacaggtt atctgttttt
     5281 acttagcaca gcttttttta cagcgatatc atacgtgtgc ggaaaggctg taaataaaga
     5341 cctggggcct gatgtggtaa cattctactg gtttttcggc gcattcttgt tttctctttt
     5401 tgtgcttccg ttatttaaag cccaaaggaa cgaattcaag aatatcggca aatacaagat
     5461 gatctttttg ttaagctcaa ttgttacctc tataggtgcg gctctgtgga tattctcact
     5521 aaggtccatt ggcctgccgc ttacttcatt tcttatgaaa gcacagaccc tttttactct
     5581 ctttttgggg ataatatttc taaaggaaaa actgaataaa ggtgaaacaa tcgggataat
     5641 aattacaata attggaggcg taatcgtagc ctatcaaagg gaaggatatc tgatcctggg
     5701 tacagcaacg gctcttaccg ctgcattctt ctattctgta ttatcttttg cggtcaagaa
     5761 atatgcctct gatctcaata tgcttactgt tgcaaatttg aggactatgg gagtgtcgat
     5821 agttgtgttt atttacctgc taatcctggg aaagtttgag cttccctcgg gcattgattt
     5881 catatacatg gcccttggtg gtcttacagg cgcttatatt gcaaaggcaa gccagttcca
     5941 atccataaag cttcttggga ttgccagatc tacggccgta atgccgcttg agtccatatt
     6001 tgtgattatc ttttcatata cgatttttaa tgaactgccc tctgcaatca aactcgctgg
     6061 tggatttatt attgtatttg gtgtttattt tcttgtgata tataggggag gggagcaaaa
     6121 aaactaaacc tttaattttt gctcataatc agtgttcagc ttgtttttct cttcatgttt
     6181 atccatagca agctctatta atgtagtgac aagcttttta tattttattc cagataattc
     6241 ccacagtttt ggatacatgc tgatctgagt gaaacccggg atagtattta tttcgctaac
     6301 aaagattctc tgagattctg attccattag aaaatctatt cttccaagcc ctgaacaatc
     6361 taaagcttta tatgcggcta ctgctatgtc ctgcagcttt tttgtgattt gcttgctaag
     6421 ttttgcaggg gcaataagct cggtggcgtc gtctgagtac ttggcttcat agtcataaaa
     6481 acctcttttg ggaacaattt ctcctgctac cgaagccttt ggatcttggt ttcctaaaac
     6541 actcacctca atctctcttg ggtctttcac agctttttca acaagtatcc tatctgaaaa
     6601 tttgagtgag tactctatag atttctttaa atctttactc gaatcgactt tagtgattcc
     6661 tatgctggaa ccaagatttg ctgcttttac aaaacaagga aagccgatct cggccttaat
     6721 ttttctaaga atgctgtttg aatttttatt ccaggtacct atatcaaatg cagtaaaatc
     6781 aacaatgggt atgccataat cttttagcac tgacttcatg gatattttat ccatacctat
     6841 agctgatcca tgcacattgg caccaacata tggaacattc attatttcaa agagaccctg
     6901 tattgcaccg tcttcaccat aagtgccatg taagatggga aatgcgcaat cgatgctgat
     6961 cttttttgtc ttttgtttgg ga
//
LOCUS       DBNGSV010000035         6217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_4604, whole genome shotgun sequence.
ACCESSION   DBNGSV010000035 DBNGSV010000000
VERSION     DBNGSV010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_4604"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="AC159U_01375"
     CDS             <1..2116
                     /locus_tag="AC159U_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3335738.1"
                     /translation="VDYWLEIKNGKKYYEARLIPLTKHQIIVIIRNSTEAKEAEGNLR
                     ESEERIRLIIENALDAVIMIDEKGTVIDWNSQAEIIFGWSKKEIIGNTMSDMIIPIQH
                     RNAHKKGLRNFLKTGESKILDKRIEITALRKNGEEFPVELSVSPIHMRDKLVFSAFVS
                     DITLRKKAEDNLIRKNKYESIISSVTKSVHSSIDLDKVLQSASDSICTNMDGVDAVAI
                     YIKEDMKAILKAHTGLTSDYIKSAGTIQYPKGFTWKTLRYSKPIYCKDASKDKHIGPA
                     GIRLGTKSYISLPLTYEGMPVGTVNTHSYSKNAFDDEQLNMLKIVIQQIEVAIRNANK
                     ANELRQSEERYRTLFDQSPIGICIFDKDMTITECNDAMVRIFQSSRDKIIGLNMHKLK
                     DKTFLPIVEDAIKGKIAYHESFYQASTSNAKLHLLASVVPIKDEAGNVDSVMGVVEDI
                     TQRKKDENEIRSLNIDLEKRVAERTTQLRAANNELEAFSYSVSHDLRSPLRALDGFSQ
                     AVLEDYHDKIDDKGKDYLTRIRKASQRMSDLIDDLLNLSRLTRSDMEFADVNLSKLVS
                     DKIDELKRIEPTRKIKVNIKNNVNVTGDPRLLNIAIENLIGNAWKFTSKSTNPMIEFG
                     ISNNSDKLIYYIKDNGAGFNMEYAGKLFGPFQRLHSDQDYRGSGIGLATVQRVITRHG
                     GKIWAEGKEGKGATFFFTLTEA"
     gene            2124..2573
                     /locus_tag="AC159U_01380"
     CDS             2124..2573
                     /locus_tag="AC159U_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3335739.1"
                     /translation="MGNKKDKVILLVEDNPDDEELTLRALKKNNIRNEIVVARDGVEA
                     LDYLFGKGKYEGRDTSDTPQLILLDLKLPKIDGIEVLKQIRSDDRTKLLPVVILTLSK
                     EQQDLVNGYSYGANSYIRKLVNFKEFIEAVGQLGLYWLVLNECPNDK"
     gene            2581..4107
                     /locus_tag="AC159U_01385"
     CDS             2581..4107
                     /locus_tag="AC159U_01385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3335740.1"
                     /translation="MQKKLKLLLIEDSLDDTKLLIRQIEKSGYELEYTRVETEKQLKG
                     ALECEKWDFVISDYSLPNFTGLSAINIIKQLGVDIPIIIVSGAIGEDIAVEAMKAGAH
                     DYIMKDNLARLSPAIERELREVQIREERRQAVNSLRESEQKYHNLFDQSPVGVFIVNK
                     DLVITESNKRAADIFLTNLEHLIGSDIRKLVDKSLISLINQAFKGKSSQKDIHYRSEF
                     DQDDTWLYCCVSPLKDSGKKVTDVMGVLEDITQRKHAEQGIQKLNINLEKRVELRTHE
                     LTNTNLKLLNEINHRKEAEKKLKSSLKEKEVLLKEVHHRVKNNLQLISSMIGLQMDGL
                     EDSNSLYMLEESERRIQSIALIHEHLYSSEDFEHINFSDYISLLVENLYSSFQIYPGH
                     IETKMELDEFSLDIDKAVPCSLIINELISNSLKHAFPLKPLKSSKSNNIIDIKARMLK
                     NSDIQITISDNGVGFPKDIDYKKTNTLGMQLVCSLVDQLHGTIKLSSKAGTKFSINFH
                     "
     gene            complement(4094..>6217)
                     /locus_tag="AC159U_01390"
     CDS             complement(4094..>6217)
                     /locus_tag="AC159U_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3335741.1"
                     /translation="SGMAAALEAKKNNLNFEIFEATEPFSTVVNFPKGKPIYTYPTDM
                     VPRGDLQFTAEVKEPLVEELKAQTLDKGVVPKIKRVEKVEKKGDTFELHIPKEDNLKA
                     RRVIVGIGRSGNFRKLGIPGENLDKVSNRLHDPKDYCSKNVLVVGGGDSALETAISIA
                     QCGGYVTLSYRKEEFSRPKPENIEKINMLREDPMADVAIETPTSERVTTSSGNFLGED
                     RKPGSINLIMKSSVRQISESEVKLLNSEGNEVAIPNDAVFSMIGREAPLEFFRRSGVK
                     ISGELGIKGLVSMIAFLLFCAFVYTWKAGGTVTKFFQERNTFPHNIPDIFVQLGAKMD
                     ELVSIPVTVTGSLAITVSEPGFYYSLLYTSLVVIFGYKRIKRRKTPYIKLQTISLACV
                     QVIPLFLLPYFVLPLMGIYGVFDSGVIKTIADELFPQVTYGHGREYWRAFGFILAWPL
                     FIWNVFSYQPLWGWLIISLIQTFVIIPFIIYRWGKGAYCGWLCSCGAMAETLGDTHRH
                     KMPHGAKWNRLNMIGQVILLLAFILLITRVVSWATPDSEIGQSIRGFYEGFLYGWSFK
                     GIELNYKWIVDLLLAGIVGYGFYFWYSGRVWCRFMCPLAALMHIFARFSKFRIIADKK
                     KCISCNVCTSVCHQGIDIMNFANKGLPMDDPECVRCSACVQSCPTGVLQFGQIDTKTG
                     QTLKVDGLAASPVLMKESAVSISEN"
BASE COUNT         2156 a         1149 c         1196 g         1716 t
ORIGIN      
        1 tgtagactac tggctggaaa taaaaaatgg taaaaaatat tatgaagcaa ggttaatacc
       61 gctcacaaaa catcagataa tcgttatcat tagaaattct acagaagcaa aagaagcgga
      121 gggaaaccta agagaaagtg aagaaagaat tagacttatc attgaaaatg cactcgacgc
      181 tgtgataatg attgatgaga aaggaactgt aattgattgg aactctcagg ctgagataat
      241 ttttggctgg tccaaaaaag aaatcattgg caacaccatg tctgatatga ttatacctat
      301 tcaacaccgc aatgcccaca aaaaaggact tagaaacttc ttaaagacgg gtgaaagcaa
      361 aattttagat aagagaattg agatcacagc acttcgtaaa aatggtgagg agttccccgt
      421 ggaactttct gtatctccta ttcacatgag agataaatta gtatttagcg cgtttgtaag
      481 cgatataact ttacgcaaaa aagctgagga taatttaata agaaaaaata aatatgaatc
      541 cattattagt tctgtcacaa aaagtgtgca ttcttcaata gacttagata aagtcctgca
      601 aagcgcttct gattctatat gtacaaatat ggatggtgtt gatgctgtag caatttatat
      661 taaagaagat atgaaggcca tattaaaagc acatacagga ctaaccagtg attatataaa
      721 aagtgcagga acaatacaat accccaaggg gtttacctgg aagaccctta gatatagtaa
      781 accaatttac tgtaaagatg caagtaagga taaacatatt ggacctgcag gaataagact
      841 tggaactaag agctatattt ctttgcccct tacttacgaa gggatgccag tgggaactgt
      901 aaacacgcat tcatacagca aaaatgcatt tgacgatgaa caattgaata tgctgaaaat
      961 agttatacaa cagatagaag ttgctataag aaatgcaaat aaagccaatg aattaaggca
     1021 atcagaagaa cgatatagaa cattattcga tcaatcccca atcggtatat gtatttttga
     1081 taaagacatg actattacag agtgcaatga tgcaatggta agaatttttc agtcatcacg
     1141 tgacaaaatt ataggtctta atatgcataa gcttaaggac aaaacatttc ttcccattgt
     1201 tgaagatgct ataaaaggaa agatagcata tcatgaaagc ttttatcagg catcaaccag
     1261 caatgctaaa ttgcacttac ttgcttcagt ggtacctata aaggatgaag caggtaatgt
     1321 tgatagtgtg atgggagtgg tagaagatat aacccagaga aaaaaagatg agaatgagat
     1381 aagaagtcta aatattgatt tggaaaaaag ggttgctgaa aggactactc agctaagagc
     1441 tgctaacaat gagctggagg catttagcta ttctgtatcc catgacctaa gatcaccact
     1501 aagggcattg gatggattta gtcaggcagt tctcgaagat tatcatgaca aaattgatga
     1561 taagggaaaa gactatctga cgcgtataag aaaagcctcg caacgaatgt cggatctcat
     1621 agatgacctg ctaaacctat caagactaac ccgtagtgac atggaatttg cagatgttaa
     1681 tctatcaaaa ttagtgagtg acaaaataga tgaactcaaa agaatagagc caacacgtaa
     1741 gattaaagta aatattaaaa acaatgtaaa tgtgactggt gatcccagac ttctcaatat
     1801 agcaatagag aatttaatag gtaatgcatg gaaatttact tcaaaaagca ctaatccaat
     1861 gatagaattt ggaataagta ataattcgga caagcttatt tattatataa aagataatgg
     1921 tgctggattt aatatggaat atgcagggaa gttgtttggg ccgtttcaaa gactacattc
     1981 tgatcaggac tacaggggaa gcggtatagg gcttgccaca gtgcaaagag taatcacaag
     2041 acatggtgga aaaatctggg cagagggtaa agaaggtaaa ggagcaacat tcttttttac
     2101 attaacggag gcttaaatat taaatgggta ataagaaaga taaagtgatt ttgcttgttg
     2161 aagataatcc ggacgatgaa gaacttacgc taagagctct taagaaaaat aatatcagaa
     2221 atgaaattgt ggttgcaaga gacggagtcg aggctttgga ctatctgttt ggtaaaggca
     2281 aatatgaggg cagagataca tctgatacac ctcaacttat attgctcgat ctgaaactcc
     2341 ctaaaattga cgggatagaa gtgttaaaac agattaggtc agatgatagg acaaaacttc
     2401 tgcccgtagt tattcttaca ctttccaaag agcagcagga cctggtaaac ggatacagtt
     2461 atggtgctaa cagttatatt agaaaactcg taaattttaa agaatttatt gaagccgtgg
     2521 gtcaattggg tttgtattgg ttagttttaa acgaatgtcc aaatgataaa tagtaaaaaa
     2581 atgcaaaaga agttaaagtt attactgata gaggattctt tagacgacac caagctgctt
     2641 ataagacaga tagaaaaatc tggatatgag cttgaataca caagggtaga aactgaaaag
     2701 caattaaagg gcgctctgga gtgtgaaaaa tgggattttg taatatctga ttattcactt
     2761 cccaacttta cgggtctttc cgctatcaat atcataaagc aactgggcgt tgatatacct
     2821 attattatag tgtcaggtgc aataggagaa gatattgcgg ttgaagcaat gaaagcaggg
     2881 gctcatgact atatcatgaa agataatctt gcaagactta gccctgccat tgaaagggag
     2941 cttcgtgaag tacaaattag agaagagcgt aggcaggcgg taaactctct tagagagagc
     3001 gagcaaaaat atcacaatct tttcgaccag tcacccgtag gggtttttat tgtaaataaa
     3061 gatttggtaa ttacagagag taataaaaga gctgccgata tatttcttac taaccttgaa
     3121 cacttaatag gatcagatat cagaaaactt gttgacaagt cactcatatc actcatcaat
     3181 caagcattta aaggtaaatc ctcacaaaag gacattcact acagatctga attcgatcag
     3241 gacgacacct ggctttattg ctgtgtatca cctctaaaag actccggcaa aaaagtaaca
     3301 gatgtaatgg gagtgctcga ggatatcact caaagaaaac atgccgaaca gggcatacag
     3361 aaactaaata ttaatttgga aaaaagggta gaactacgca cacatgaact tactaacaca
     3421 aatttaaaat tattaaatga aatcaatcac cgtaaagaag cagaaaaaaa gctcaagtca
     3481 tcgttaaaag aaaaagaagt gctcttaaaa gaagtccacc atagagtcaa aaataattta
     3541 cagcttatct cgagcatgat aggactgcaa atggatggtc ttgaggacag caactcactt
     3601 tatatgcttg aagagagtga gagaagaatc cagtccatag cccttataca tgaacatctt
     3661 tatagttcag aagactttga gcatataaac tttagtgatt atatatctct attagttgaa
     3721 aatctctaca gttcattcca gatttatccg ggtcatatag aaacgaaaat ggagcttgat
     3781 gaatttagct tagatattga taaagctgtg ccatgcagcc ttattattaa tgaactcata
     3841 tctaactctc taaagcatgc atttccacta aaaccattaa aatcttctaa gagtaataat
     3901 ataatagata ttaaagcccg catgctgaaa aactcagata tacagattac aattagtgat
     3961 aacggcgttg gatttccaaa ggatattgat tataaaaaga ccaatactct tggaatgcag
     4021 ctagtatgca gtcttgtaga tcagcttcat ggcactataa agctatcaag caaagcaggt
     4081 accaaattct caatcaattt tcactaattg atactgcact ttctttcatt agcactggtg
     4141 aagctgcaag accgtcgact tttaatgttt gtcctgtctt agtatctatc tgaccaaact
     4201 gaagcacgcc tgtggggcag gactgaacac acgcactaca cctgacacac tcggggtcat
     4261 ccatcggcag acccttgttt gcaaagttca taatatctat tccctgatgg cacactgatg
     4321 tgcaaacatt acacgatatg catttctttt tatcggcaat aatcctgaac ttactaaacc
     4381 ttgcaaaaat atgcataagc gctgccagtg ggcacataaa cctgcaccat actctgcctg
     4441 agtaccagaa ataaaatcca tatccaacta ttcctgcaag aagcagatct actatccatt
     4501 tataattaag ctcgatgccc ttaaatgacc agccatataa aaaaccttcg taaaaacctc
     4561 ttatagactg gcctatttca ctgtcaggtg ttgcccatga tacaactctt gtaataagca
     4621 gtatgaatgc cagcagaagt ataacctgtc ctatcatgtt tagcctgttc cattttgcac
     4681 cgtgaggcat cttgtgtctg tgtgtatcac ccagtgtttc agccatagcg ccgcatgaac
     4741 aaagccagcc gcagtaagcg cccttacccc agcggtatat aataaaagga atgataacaa
     4801 aggtttggat cagactaatg attaaccatc cccaaagagg ctgatagcta aagacgttcc
     4861 agataaatag cggccaggca agtatgaatc caaacgccct ccagtattcc cgtccatgac
     4921 catatgtaac ctgtggaaat agctcatcgg caatggtttt aataactcct gaatcaaaaa
     4981 caccataaat acccataagc ggcagcacaa aatacggaag caaaaacagg ggaattacct
     5041 gaacacaagc aagtgaaatt gtttgtagct ttatataagg agtttttctt cttttaattc
     5101 ttttgtaacc aaatataacg accagacttg tatacagaag cgagtaataa aagcctggtt
     5161 cagaaactgt aatagcaagt gagcccgtga cagtcacagg aattgagaca agctcgtcca
     5221 tctttgcgcc aagctgtaca aatatatccg gtatattatg aggaaaagta tttctttcct
     5281 gaaagaactt tgtaacagta cctccggcct tccatgtata gacaaatgca cagaaaagca
     5341 aaaacgcaat catcgacacc aagcctttta ttccaagctc accagaaatt ttaactccgc
     5401 ttcttctaaa gaactcgagc ggggcttctc tgcctatcat tgaaaatact gcgtcatttg
     5461 gaattgcaac ttcgtttccc tcagagttta gtagttttac ctcactttca cttatttgcc
     5521 ttacactact tttcattatc aggttaatgc ttccaggctt tctatcctcg cccagaaaat
     5581 taccagagct tgtagttact ctctcagagg tcggcgtctc aatagcaaca tcggccatgg
     5641 gatcttctct gagcatgttt attttttcaa tattctcagg ttttggcctt gaaaattctt
     5701 ctttacgata agagagtgtc acgtacccgc cacactgtgc aatagaaata gcagtttcaa
     5761 gtgcactgtc tcccccaccc actacaagta catttttgct gcaatagtcc tttgggtcat
     5821 gaagcctgtt tgatacctta tcaagatttt ccccgggtat accaagcttc ctgaaatttc
     5881 ctgaacgccc tataccaacg ataacacgtc ttgcttttag attatcttct ttaggaatat
     5941 gtagttcaaa cgtatcccct ttcttttcaa ctttctccac tctcttaatt ttaggaacaa
     6001 cccctttatc aagagtctgg gcttttagct cctctactag aggttctttt acttctgctg
     6061 taaactgcag gtctcccctc ggtaccatat cggtaggata tgtgtagatg ggctttcctt
     6121 ttgggaaatt tactaccgta gaaaacggct cggttgcctc aaatatctcg aaattaagat
     6181 tatttttctt ggcttcaagc gccgctgcca tccctga
//
LOCUS       DBNGSV010000036         1700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1874646, whole genome shotgun sequence.
ACCESSION   DBNGSV010000036 DBNGSV010000000
VERSION     DBNGSV010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1874646"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="AC159U_01395"
     CDS             <1..1623
                     /locus_tag="AC159U_01395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3335742.1"
                     /translation="NELESLSERTGKLLSFAYLMFAGDTNDPKTGAFLQQMQETATEI
                     RKHLLFFELEWIRVSDDKANALINHEKLKSYDHFLENERKYKPHRLLEGEEKVLDIKS
                     NTGGRAFKRLFDEVVNNIEFKVRLDGKTKPLTETETLSLQYDPDREKRKAGAKGLTKG
                     LKQNARVLTFIFNTLIKDHESTDSLRSYDYAMQARNMDNEIDKKTVDALLKASEKNFD
                     IVARYYDLKKQILGMDKFYDYDRYCPIMENKRNIPYTRAKEIVLQSFAEFSTEMHDIT
                     KQFFDKNWIDSEVREGKRGGAFSHSTVPSVHPYVFTNFNGKMRDVMTLAHELGHGVHQ
                     YLSRQQGYFHSDTPLTTSETASVFAEMLVFKKLMDDEDDPNEKLALLCGKLEEMFATV
                     FRQVVMTRFEQSVHDASRKKGELSTEDFNKLWIKTNRKMFGKSVTLTKDYSYWWLYIP
                     HFVHSPFYCYAYSFGELLVLSLYKKYLKEGADFVPKYMELLASGGMDSPNNLLEKLNI
                     DISNPEFWQEGLDYLSDILGEAETLSSQIKNN"
BASE COUNT          579 a          314 c          356 g          451 t
ORIGIN      
        1 aatgagcttg aatcgctcag tgaaagaaca ggcaagctgc tatcctttgc ttatcttatg
       61 tttgctggtg atacgaatga tcccaaaacc ggagcgttcc ttcagcaaat gcaggaaacc
      121 gctaccgaaa taagaaagca tctcttgttt tttgagcttg aatggataag ggtttcagat
      181 gataaagcaa acgctcttat aaaccacgaa aagctaaaaa gctacgacca ttttttggaa
      241 aatgagagaa agtacaaacc ccacagatta ttagaaggag aagaaaaggt attagatata
      301 aaatcaaaca ctggaggtcg ggcgtttaaa aggctctttg atgaggtagt aaataatatt
      361 gagtttaaag taaggctcga tggcaaaaca aaacccctta ctgaaaccga gaccctttca
      421 ctccaatacg accctgacag agaaaaaaga aaggctgggg caaaaggact taccaaaggg
      481 cttaaacaaa atgcccgggt tctcacattt atctttaata cgcttataaa agaccatgag
      541 agcactgaca gtctcaggtc atatgactat gctatgcagg cccgcaacat ggacaacgag
      601 attgataaaa aaactgtgga cgcactgcta aaagcatcag aaaagaactt tgatatagtt
      661 gccagatact atgatcttaa aaaacaaata ctgggcatgg ataagttcta tgattatgac
      721 cgttactgcc cgattatgga aaacaaaaga aatattccct acacgagagc aaaagagata
      781 gtactgcagt catttgcaga gttttctaca gagatgcatg atattactaa acagttcttt
      841 gataaaaact ggatagattc tgaagtaagg gaagggaaaa gaggtggagc ttttagccac
      901 agcaccgtgc caagtgtaca cccatatgtg tttacaaatt ttaacggaaa gatgagagac
      961 gtgatgaccc ttgcccatga gttaggacac ggggtacatc agtatttatc aaggcagcag
     1021 ggatactttc atagcgatac ccctcttaca acatctgaga ctgccagcgt ttttgctgaa
     1081 atgctcgtat ttaaaaaact tatggatgat gaagatgatc caaacgaaaa attggctcta
     1141 ctgtgtggta agctagaaga gatgtttgct actgttttca ggcaggtggt aatgacaaga
     1201 ttcgagcaga gcgtacatga tgcctcacgt aaaaaaggcg agcttagtac agaagatttt
     1261 aacaagctgt ggataaaaac caacagaaag atgtttggta aatctgtaac ccttacaaaa
     1321 gattacagct actggtggtt atatattcca cactttgtac attctccttt ttactgctat
     1381 gcatattctt ttggagagct tttggtgctt tctctataca aaaaatacct taaagaaggc
     1441 gctgactttg ttccaaaata tatggaactg cttgcctccg ggggtatgga ttcgcctaac
     1501 aacctgcttg aaaagctgaa tatagatatt tccaatcctg agttttggca ggaaggcctg
     1561 gattacctat ctgatattct tggtgaagct gagactctaa gctctcaaat taaaaacaat
     1621 taaatttgag taaatacatg tttctaaccc taattaccca ccttattgcc tatgaaaata
     1681 cgtgtggtag tataagcccg
//
LOCUS       DBNGSV010000037         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_74324, whole genome shotgun sequence.
ACCESSION   DBNGSV010000037 DBNGSV010000000
VERSION     DBNGSV010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_74324"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC159U_01400"
     CDS             complement(<1..395)
                     /locus_tag="AC159U_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HIR3335743.1"
                     /translation="MSKKLLVIGGSGELGYQIIKNAGSWKTFATYYSNKLNLSNIESF
                     KLDITNKNEIEELITNIKPHIVINCAISDRSINNIEDDEDKKFAIINGSLNVANTCNS
                     IGSRAIFISTDLVFDGKKGNYTESDKPNPV"
     gene            466..969
                     /locus_tag="AC159U_01405"
     CDS             466..969
                     /locus_tag="AC159U_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3335744.1"
                     /translation="MLDAQKIIEMFDLKPLPQEGGFYKESYRSDDKILKQALPERYSG
                     DKNFGTAIYYFLTPETKSLMHRLTSDEIFHFYLGDSVEMLQLFPDSSGKIITLGSNIT
                     QGHLVQVLVPNAIWQGMRLRPGGSHALMGTTMAPGFNFDDFELGSRRNLVEQYPDFKD
                     QIIKLTN"
     gene            973..2142
                     /gene="xseA"
                     /locus_tag="AC159U_01410"
     CDS             973..2142
                     /gene="xseA"
                     /locus_tag="AC159U_01410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3335745.1"
                     /translation="MEDFPSFQGLISGDIFTVSQINERIKLSIEDEFGLDFVWIVGEI
                     SNFRGNYSSGHWYFTLKDENTQISAVCFKWANQYIKFNPENGMEVICCGQINVYEKQG
                     SYQINIRYIEPKGIGAQALALEQLKEKLFAEGLFDESRKRELPFLPETIGIVTSPTGA
                     AIKDILKVLDRRFADLHIIISPAGVQGKEAAADIVSALNILYKEKYLDEIIVARGGGS
                     KEDLMVFNEESVVRAIAKSPVPVISAVGHEIDITLTDLVADVRAATPSMAAEIAVREK
                     ADLVYTLDEIKSRLLSSLVGFQQTWNKEISDLYDDLTRALRYKLESTAMELGSISGQL
                     DALSPLKVLDRGYSITQKIPGNKVIKDSKKLQKNDEVLIRFLKGTAKCKVEETKS"
     gene            complement(2139..>2481)
                     /locus_tag="AC159U_01415"
     CDS             complement(2139..>2481)
                     /locus_tag="AC159U_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335746.1"
                     /translation="SSEDQSFSYLLASELYEKIIHLLAICLGIRLVIAEDKECFYEDI
                     LEAKPTILFETKSGIEQICNNLNGTGLKHMLGGRVRYLITDEEPAVDISNRLKRENIN
                     VIAVQQNAKPN"
BASE COUNT          799 a          445 c          476 g          761 t
ORIGIN      
        1 accggattcg gcttgtctga ttctgtgtaa ttacctttct taccatcaaa tacaagatct
       61 gtagaaataa aaatagccct gcttcctatg ctattacatg tattggcaac attgagagag
      121 ccattaataa tagcaaattt tttatcttca tcatcttcta tattattaat agaccgatca
      181 cttatggcac aattaattac tatatgcggt ttgatatttg ttattaattc ttcgatttca
      241 ttcttgttag taatatcaag cttgaatgat tcgatattac tcagattcag cttgttagaa
      301 tagtaagtgg caaatgtttt ccagctaccc gcatttttta ttatctgata gcctagctct
      361 ccactcccac ctattaccaa taacttttta ctcatttgat ttttataaaa ttcaatttgt
      421 aatgtaatat ctaattttaa tttcagttgt taattatact ttgtaatgct tgatgcccaa
      481 aagatcattg aaatgtttga tttaaaaccc ctgccccagg aaggaggatt ttataaagag
      541 tcatacaggt ctgatgataa aatattaaag caggcattgc ctgagagata ttcaggtgat
      601 aaaaattttg ggacggctat ttattacttt ctcacacctg agactaaatc tcttatgcat
      661 agattaactt ccgatgagat atttcacttt tatctaggag actctgtaga aatgctgcaa
      721 ctttttccag actcttcagg gaaaataatc actttaggaa gcaatataac acaaggacat
      781 cttgtacagg tcctggttcc taatgcaatc tggcagggaa tgagattaag acccggaggt
      841 agtcatgctt taatgggtac taccatggcg ccaggattca attttgatga ttttgaactt
      901 ggcagtagaa gaaacttagt tgaacaatat cctgatttta aagatcagat cataaaatta
      961 actaactaaa atatggaaga ctttccttca tttcagggct taatatcagg agacatattc
     1021 acagtctcac agataaacga aagaatcaaa ttatctatag aggacgagtt tggtttggat
     1081 tttgtctgga ttgtaggaga aatctcaaac tttagaggaa actactcaag cggccactgg
     1141 tattttactt taaaagacga aaatacccag atatctgcag tctgctttaa atgggcaaac
     1201 caatatataa agttcaatcc cgaaaacggc atggaagtaa tctgctgcgg tcagattaat
     1261 gtatatgaaa aacaaggctc atatcagata aatatcagat acatagaacc aaaaggaata
     1321 ggcgctcaag ctctggcatt agaacaactt aaggaaaaat tatttgccga aggactgttc
     1381 gatgagagca ggaaaagaga gcttcctttt ctgccggaaa caatcggtat cgtaacctca
     1441 ccaacaggag ctgccataaa agatatatta aaggtccttg atagacgctt tgctgatctt
     1501 catattatta tttcccccgc aggggttcag ggcaaagaag ctgcggcgga cattgtaagt
     1561 gccttgaata ttttatataa agaaaaatat ctggatgaaa tcatagtcgc acggggcggg
     1621 ggatcgaaag aggacttgat ggtcttcaat gaagaatctg ttgtaagagc tatagcgaag
     1681 tcgcctgtgc ctgtcatatc ggcagtcgga catgagatcg atatcacact tacagacctg
     1741 gtagcagatg ttagggctgc tacaccttct atggcagcag aaatagcagt tagagaaaag
     1801 gctgatcttg tgtatacatt ggatgaaatt aaaagcaggc tcctttcatc tcttgtcggt
     1861 tttcagcaaa catggaataa agaaatttcg gacttgtatg acgatctaac tagagcgctt
     1921 cgttacaagt tagagagtac agcaatggag cttggctcta tttcaggaca gttggatgct
     1981 cttagcccct taaaagtttt agacagagga tatagcatta ctcaaaagat tcccggaaac
     2041 aaagtaatca aagactcaaa aaaactacaa aaaaatgacg aagttttgat tagatttctt
     2101 aagggaactg caaagtgtaa ggtggaagaa actaaaagct aatttggctt tgcattttgc
     2161 tgtactgcta taacgttaat gttttcacgt tttagcctgt ttgatatatc aaccgccggc
     2221 tcttcatccg taatgagata ccgcaccctg ccgcccagca tgtgttttaa gcctgttccg
     2281 tttaggttat tacaaatttg ttcaatgccg gatttggttt caaatagtat agtaggtttt
     2341 gcttctagta tatcctcata aaaacactct ttatcttcgg ctataacaag ccttataccc
     2401 aaacaaattg ccagcaaatg aataattttc tcatacagct ccgatgcgag gagataagaa
     2461 aatgactgat cctctgatga a
//
LOCUS       DBNGSV010000038         2717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_974768, whole genome shotgun sequence.
ACCESSION   DBNGSV010000038 DBNGSV010000000
VERSION     DBNGSV010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_974768"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="AC159U_01420"
     CDS             <1..868
                     /locus_tag="AC159U_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR3335747.1"
                     /translation="DATLNKTRITITQQKKQQVSEIKFVDQETNENDEIVIKDEEKGS
                     YFKDHMALDLNVTIPKNSWVRGRGANIELKGKLKIDKEYKDEPLITGNISTVRGTYKI
                     FGKLFKIQEGNVNFPAIKDLNPLLDITALYNVSDVDVFVGIGGDVKNPEIKLTSNPSL
                     QETDIVSYLIFGTSSDKLGSGERSALGSAATGLAGGVALNQLKGVLGDNVSPDVLSFG
                     SGESGPELEVGKYLNDDIYFAYERKSSSDSGSSIPTNTVKVEYRLFDFLSVGSDVGSE
                     QSGGDIFFNFEY"
     gene            complement(888..1550)
                     /locus_tag="AC159U_01425"
     CDS             complement(888..1550)
                     /locus_tag="AC159U_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3335748.1"
                     /translation="MLLERRIETGNKTNYLSTDLDYNNIADEKLAALYVNGRDEAAFN
                     ELVNRFGDKIYRLAMRITKSPESAEEVLQRVFVKLVGKLRLFRGDSKLSTWIYTVSSN
                     EAFRYLRERNGSREYSLDELSDKQSDEHKGWLQPRDTNPDPESNAINTEISEILDNAI
                     NELQEEYRTVFQLRDVEGLSNCEVAEILGLSIPAVKSRILRARTQLKDKLSKQISKDI
                     IS"
     gene            complement(1626..2630)
                     /locus_tag="AC159U_01430"
     CDS             complement(1626..2630)
                     /locus_tag="AC159U_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3335749.1"
                     /translation="MKQVKSFLTKTLVGTIVVISVLFITLGYLEAAESNHETKPNEHK
                     TLHKTVEIDGLEIFYREAGPKDAPTILLLHGFPTSSHMFRNLIEELSDDFHLVAPDYP
                     GYGFSSMPTVDEFDYSFDNLANIVHKFTKKIGLENYSLYLQDYGAPVGFRLFTKNPNK
                     VEALIIQNGNAYDEGLRDFWVPLKAYWKDKSDKNAEPLKGFLTLDVTKWQFTHGVRNP
                     QTISPDNWGHVQPLLDRPGNQAIQLELFYSYGTNPPLYPKWQAYMRNYQPPTLIVWGK
                     NDYIFPAEGAYPYKRDLKNVDFNILDTGHFALEEDLDVIAKHIRDFYGKKVAMKSRIK
                     "
BASE COUNT          768 a          509 c          509 g          931 t
ORIGIN      
        1 agatgcaact ctaaacaaga cacgaataac tattacgcag cagaaaaaac aacaggttag
       61 tgaaattaaa tttgttgatc aagagactaa cgaaaatgat gagattgtga tcaaggatga
      121 agagaaagga agctacttta aagaccatat ggctttggat ctaaatgtca cgattcccaa
      181 gaacagctgg gtgaggggaa ggggggctaa tattgagctt aaaggtaaac tcaaaattga
      241 taaggaatat aaagacgaac cacttataac gggaaatata tcgacggtta gaggtacata
      301 taaaatattt ggaaaactgt ttaaaataca ggaaggaaat gtgaatttcc cggcgataaa
      361 agatcttaac cctcttctcg acattactgc tctttataat gtctcggatg tggacgtgtt
      421 tgtaggtatt ggtggagatg ttaaaaatcc tgagattaag ttaacaagca atccttcact
      481 gcaggaaact gatattgttt catatttaat ctttggaaca tcgtctgaca aacttggaag
      541 tggtgaaaga tctgcacttg gatcagcggc tacaggtctt gccggtgggg tagctcttaa
      601 tcagcttaag ggagttttag gcgacaatgt ttcccctgat gtactctcat tcggatcagg
      661 tgaatccggt cctgagcttg aagtaggtaa atacttaaat gatgatatct attttgccta
      721 tgagaggaaa tcatcatctg attccggctc atctattcct actaatacag ttaaagtgga
      781 atatcgattg tttgactttc tttctgtagg cagtgatgta ggaagcgagc aatccggcgg
      841 tgatatattt ttcaattttg aatattaact ttatgactct tttcctttca tgatatgata
      901 tctttagaaa tctgcttaga taatttgtct ttaagctgag tccttgccct aagaatcctt
      961 gatttaacag caggaataga tagtccaaga atttctgcaa cttcacaatt ggataaaccc
     1021 tcaacatctc ttagctgaaa cacagtgcga tattcctctt gcagttcatt aattgcatta
     1081 tccaatattt ctgatatctc agtattaata gcattgcttt cagggtctgg attggtgtcc
     1141 cttggttgca accagccttt atgttcatca ctttgtttat cacttaattc gtccagactg
     1201 tattctctcg aaccattcct ttctcttaaa tacctgaatg cttcattgct gctaactgta
     1261 taaatccagg ttgataactt tgagtcacct ctgaataatc ttagcttccc aaccaattta
     1321 acaaataccc tttgtaaaac ctcttcggca gattcaggag atttggttat cctcattgca
     1381 agcctgtata tcttgtctcc aaatctgttt acaagttcat tgaatgctgc ttcgtctctt
     1441 ccatttacat atagtgcagc caacttctca tctgctatat tgttgtagtc taaatctgta
     1501 cttagataat ttgttttatt tcctgtctct atccttcttt ctaataacat ttttaacccc
     1561 cttttacttt tatattcaac tttgccggat gcttttatgt aagcacccgg ccagtttttt
     1621 acttgttatt taattcttga tttcattgca acctttttac cgtagaaatc tcttatgtgt
     1681 tttgctatca catccagatc ttcttctaat gcgaagtgtc ctgtgtccag tatattaaaa
     1741 tctacgtttt tcagatctct tttgtatgga taagcccctt ctgccgggaa gatataatca
     1801 ttcttacccc agactatgag tgttggcggc tgatagtttc tcatatatgc ctgccatttt
     1861 ggatatagcg gagggtttgt gccgtagctg tagaaaagct caagctgaat tgcctgattg
     1921 cccggacggt caagcagcgg ctgtacatgt ccccagttgt cagggcttat ggtctgcggg
     1981 tttcttactc catgagtaaa ctgccatttg gttacatcga gcgtaagaaa accttttaga
     2041 ggttcagcat ttttatctga tttatccttc cagtaagctt ttaaaggaac ccagaagtct
     2101 cttaggcctt catcatatgc attaccgttt tgaataataa gggcctctac tttatttgga
     2161 tttttagtaa aaagtctaaa tcctacggga gcgccatagt cctgtagata cagactgtaa
     2221 ttttcaagtc ctattttttt tgtgaattta tgtactatat ttgcaaggtt atcaaaactg
     2281 tagtcaaact catccactgt aggcatggag ctgaagccat agccaggata atccggggca
     2341 accagatgaa aatcatccga aagctcctct attaggtttc taaacatatg agatgatgtc
     2401 ggaaaaccgt gaagtaaaag tatggtcggt gcatcttttg gccctgcttc tctatagaat
     2461 atttcaagcc cgtctatctc tactgttttg tgaagggtct tatgttcatt gggttttgtt
     2521 tcgtggtttg actctgcagc ctctaaatat cctagggtta taaaaagaac tgagattacg
     2581 acaattgttc ctactaatgt ctttgttaaa aatgatttaa cttgtttcat tattattctc
     2641 cttttattag atttatttat atgcattaaa catacagaca agtctgtact atttaagata
     2701 aaaaaatata tcttagg
//
LOCUS       DBNGSV010000039         2672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_974864, whole genome shotgun sequence.
ACCESSION   DBNGSV010000039 DBNGSV010000000
VERSION     DBNGSV010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_974864"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2228)
                     /locus_tag="AC159U_01435"
     CDS             complement(114..2228)
                     /locus_tag="AC159U_01435"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3335750.1"
                     /translation="MFNEQIKRTLILGVVCSLLVLLTSYMGVTNDFKNATFDLFSRYL
                     NPESPIDAGISSNIVIVEIDQRSLDDINKEGINWPWPRQIYAPLIEYLSEADAVFVDV
                     LFTEPSSYGFEDDKIFADAIKSNKNVYMPVFLTKSFYELSENDKKLLDRISVDSSTRT
                     YMSFKSVITPIDLIKDAVVSSGNVTLAPDGDGIYRRMSFFFRVGEYVVPHFLINFLLD
                     KNIVSLKDGKLYGNGNKLPVEDGNLILNYYSKDQAFKSFSASHIIKSYLDSQQSKTPV
                     VPKDYFKGKIVLIGLTAPGLYDLRPTTVASVSTGINIHATTIDNFYHNRFIHKVPNFI
                     LYILIILICFFSIYYILQSPSLLRNLSYFVIVFSVITVVDILLFKFAIYLNHIDPLFS
                     LILSFIVAVAYSYATEGKKRELTEKTLLQYMDKQVAEYLLNNPSLIKPGGQKKRVTIF
                     FADISGFTTLAEKTSPEEISKILIDVLNLFTDEIIENKGVIDKYIGDCVMAFWGAPIE
                     SENDEINSCKAAVASIKLLEEKNKDFEKNGLPRISFRVGIHSGEAIVGNLGSDRVFDY
                     TVVGDSVNLASRMESLNKYFATNILISEDTLNKTAGLFITREIGPIEVKGKTVSTSVY
                     ELIDIKEEVSDNITTLVDKYHQALNIYKEQNFSAALQKFKDIIKEYGKDGPTEFYINR
                     CEELMSTNSLTKDWKVIKFDSK"
     gene            complement(2318..2497)
                     /locus_tag="AC159U_01440"
     CDS             complement(2318..2497)
                     /locus_tag="AC159U_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335751.1"
                     /translation="MLEVTKCKECGYEIGLKVDTCPGCGEKIGIEIGGITGLIRRISI
                     IIFALYILLSALKII"
BASE COUNT          877 a          515 c          390 g          890 t
ORIGIN      
        1 atataatcat ctgagaagac atggaggttt gaactatgaa accccttttg ataaactaca
       61 aaaagttacc gaattactga gctagtacaa cccttctctt tttacttaca acactactta
      121 ctatcaaatt ttattacttt ccagtctttt gtcaagctat ttgttgacat taattcctca
      181 cacctattaa tataaaactc tgttggcccg tcttttccat attccttaat gatatcctta
      241 aatttttgca gtgcagcact aaagttttgt tccttataaa tattcaaggc ttgatgatat
      301 ttatcaacta aagtggtaat attatcactt acttcttctt taatatcaat caactcatat
      361 acacttgtcg atacggtttt acctttaact tcaataggcc ctatctcacg agtaatgaac
      421 aggccggctg ttttatttaa tgtgtcttca cttattaata tgttagtagc gaagtacttg
      481 tttaaagatt ccatcctgga cgctagattt accgaatctc ctacaactgt gtaatcaaat
      541 accctgtcgc taccaagatt tcctacaata gcctcccccg aatgtatacc aactctaaac
      601 gatatgcgtg gaagtccgtt tttttcaaaa tccttattct tttcttctaa tagcttgatt
      661 gaggccactg cggccttaca cgaatttatt tcatcatttt cagactctat gggtgcaccc
      721 caaaacgcca taacgcagtc accaatgtat ttatctatta ctcctttgtt ttcaatgatt
      781 tcatcggtaa aaaggtttag cacatcaata agaatcttag atatttcctc aggtgaggtt
      841 ttttcagcaa gagtagtaaa tcctgaaata tctgcaaaaa atatagtaac cctctttttc
      901 tgtccgccgg gcttaatcag agatgggtta ttaagaagat actcggctac ctgtttgtcc
      961 atatactgaa gaagggtttt ttcagtcagc tctcttttct ttccttctgt agcataacta
     1021 taggcaactg cgacaataaa actcaatatg agtgaaaaaa gcggatcaat atggtttaga
     1081 tatattgcaa atttgaaaag cagaatatca accactgtta taacagagaa aacaatcaca
     1141 aaataagaaa gatttctaag tagagacgga ctttgcagaa tataataaat gctaaaaaaa
     1201 catatcaata taattaatat atacagaata aaatttggta ctttatgaat gaaacggttg
     1261 tgatagaaat tatctattgt tgttgcatga atattaattc cggtagatac cgatgccact
     1321 gtcgtaggtc ttaaatcata taaacccggg gctgtaagac caattaggac tattttccct
     1381 ttaaaataat cttttggtac taccggtgtt tttgactgct gactgtctag ataacttttt
     1441 atgatgtgag aggcagaaaa tgatttaaat gcctgatcct tactataata attaagtatc
     1501 aagtttccat cttccacggg aagtttattg ccgttaccat ataactttcc gtctttgaga
     1561 ctgacaatat ttttatccaa tagaaaattg ataagaaaat gaggtacaac atactcgccc
     1621 actctaaaaa agaacgacat tctcctgtaa atgccatcac catcgggagc aagagtcaca
     1681 tttcctgaac taactactgc atcttttatt agatcaatag gagtaataac tgatttaaaa
     1741 ctcatatatg ttctggtact gctgtctaca gatatacggt ctaataactt cttgtcattt
     1801 tcggataatt cataaaaact tttagtaaga aacacgggca tatatacgtt tttgttgctt
     1861 tttattgcgt ctgcaaatat cttgtcgtct tcaaaaccat aagaagaagg ctcagtgaaa
     1921 agcacatcca caaatacagc atcagcctct gaaagatatt caatcaatgg agcatagatt
     1981 tgccttggcc atggccagtt tatgccttcc ttatttatat cgtctaaact tctctgatcg
     2041 atctcaacta ttactatatt gctagatatg ccggcatcta taggcgattc agggttcaga
     2101 tatcttgaaa aaagatcaaa tgtagcgttc ttaaaatcat tggtaacccc catatacgag
     2161 gttaaaagca ctaaaagcga acacaccact ccaagtatta aagttctttt tatttgttca
     2221 ttaaacaaga tagttcaatt tcactccatt acacataaaa aacaggtggt tagtatatct
     2281 taaataatct gaaaaagatg ctgaatataa tcaaatatta aattatttta agtgcgctta
     2341 aaagaatata aagcgcaaat ataattatag aaattcttcg tattagccct gtaataccac
     2401 caatctcaat acctattttc tcaccacaac ccgggcatgt atcaactttt agcccaattt
     2461 cgtaaccgca ctctttacac ttcgtgactt cgagcatatg ctttccccgg taaaatcaat
     2521 aaatcctgat aatataaatt atacatggag ctcgcataga acagagctat aagtaatgca
     2581 gaatctcctt tattttatta actaaatcta catttctgtt attaaatcta aattccatct
     2641 cttttagata caaaacaaag tgttttctgt ca
//
LOCUS       DBNGSV010000040         3572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1182908, whole genome shotgun sequence.
ACCESSION   DBNGSV010000040 DBNGSV010000000
VERSION     DBNGSV010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1182908"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="AC159U_01445"
     CDS             <1..361
                     /locus_tag="AC159U_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3335752.1"
                     /translation="YPASQLFNRQMDCIRLLHNNASLRLDRMNQCNSIRVVAPLISGE
                     LLSYAIRIPSEYKLRPMQEAKIEKWIFRKAFENYLPDSIVWRPKQEFSQGAKSGLFEH
                     LILLCPNGFFFNYLLAV"
     gene            complement(540..1280)
                     /gene="istB"
                     /locus_tag="AC159U_01450"
     CDS             complement(540..1280)
                     /gene="istB"
                     /locus_tag="AC159U_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR3335753.1"
                     /translation="MIEATVEKLISMKLHGMADAVQEQMKNRAYKDLGFEERLGMLVD
                     REMDNRQDRKLRTLLTKARFRYPGASIEDINFRARRGISKDVILKLAQNGWIKDKRNM
                     IITGSTGVGKTYFACALGNSACRAGISTYYVRLTRLFHELKISRADGSYGKWLQKLSR
                     TNLLIVDDWGLSPLSDTERIDFLEIMEDRYNVRSTIISSQIPVDKWHDLIGEPTIADA
                     ICDRLIHNAHKIILTGKSMRGGDPEKSE"
     gene            complement(1273..2841)
                     /gene="istA"
                     /locus_tag="AC159U_01455"
     CDS             complement(1273..2841)
                     /gene="istA"
                     /locus_tag="AC159U_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR3335754.1"
                     /translation="MKKKSGRLCMRKILEMCRLRLKMGLGINQIAGACNISSSTASTY
                     VKKIEQLKLSYEELSSLDEEALYNLLFPATGEIAVPDKAMPDFEYLTKELKKKGVTLQ
                     LLYEEYRAGNPGGYSRSRFYEKYRHYAKKLNPVMRFNHKAGDKMFEDFSGDRPQYINP
                     ETGEAVETELFVSALGASSYTFACTVADQKVENFIKANIQALEYYGGSPRCIVPDNLK
                     SGVTSACYYDPEINRTFADMAEHYDVAVVPARAGKPRDKAKVENAVLQAQRRILAALR
                     NRTFFSLRELDEAIREEVEKLNSRPMAVTGKSRRELFEEIEKPVLRPLPAERFEIYNY
                     KKPTKLHIDYHVEVEKSYYSVPYTLIGQYVDIKYNSRVVEIYHHNRRVASHIRTYKKG
                     KYITEDSHMPHEHRQYLEWTPERIKNWGGKIGLHTKTMMHRIMESKKHPEQGFRSCLG
                     IIRLSKQYTPERVESACKRALAVEACNYGSVKSILQTGLDKVAYLDEHKAAKPIEHPN
                     IRGREYYREVMGND"
     gene            2993..3385
                     /locus_tag="AC159U_01460"
     CDS             2993..3385
                     /locus_tag="AC159U_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335755.1"
                     /translation="MGVRLTQDSVFELGRILQIESEKGMRVATDMVSCKDKKFYLAED
                     YHQKHALQRYSDLFREYRTIYPDMQYLIASTAAARVNGYLAGYGECNKLQSEIESLGL
                     SIKGMKSLLSIICRKETIFPCVGSGSLE"
BASE COUNT          808 a          895 c          740 g         1129 t
ORIGIN      
        1 ttaccccgcg agtcagttgt tcaaccgtca gatggactgc attagattat tgcataacaa
       61 cgcatctctt cggcttgacc gtatgaatca atgcaattcg atccgggtag ttgcgcccct
      121 gatttccggt gagttactca gttatgccat aagaatccct tcggaatata aattgaggcc
      181 tatgcaggaa gcaaagattg aaaagtggat attcagaaag gcatttgaaa attatctgcc
      241 cgacagcatt gtatggagac cgaaacagga gttttcccag ggtgccaaat ccggtctatt
      301 cgaacactta atcctgcttt gtccgaacgg tttctttttt aattatctcc tggctgtttg
      361 agtttaaaaa agtgttcgag ttgtgtcaat tcttttttct tgtcagtgca ccctcctttc
      421 ttcagattgt ctggatttta gcaaaacgga catatccctg aggtcgtctt tgcccgggat
      481 aatcgattac agggcaaatg tgaaagccgt tttttttggt atgacttctt ttcatatttt
      541 cattcgcttt tttcaggatc acctcctctc attgacttgc ccgtgagtat gattttgtgt
      601 gcgttgtgaa tcagcctgtc gcatatggcg tctgctattg tcggttctcc tataagatca
      661 tgccatttgt ccacgggtat ttgactggat attatggtag agcgtacatt gtatctgtcc
      721 tccatgattt caaggaaatc aattcgttcc gtgtctgaaa gaggagaaag tccccagtcg
      781 tccacaataa ggagattggt ccttgagagt ttctgaagcc atttgccata acttccgtct
      841 gcccgtgata ttttcaattc gtgaaacagg cgcgtcagcc gcacgtagta tgtgcttatg
      901 cccgcccggc aggcgctgtt tccaagggca caggcaaaat atgtcttgcc aacgccggta
      961 gagccggtga tgatcatatt ccgtttgtct tttatccatc cgttctgggc cagtttcaat
     1021 atcacatctt ttgatattcc ccttcttgcc ctgaagttga tgtcctcaat gctcgccccc
     1081 ggatagcgga accgtgcctt tgtcaaaagc gtccgaagct ttcggtcttg cctgttgtcc
     1141 atttctctgt ccacgagcat ccccagccgt tcttcaaagc ctaagtcctt atacgcacgg
     1201 tttttcatct gctcctgtac cgcatcagcc atgccgtgca gtttcatcga gatcagtttt
     1261 tctacggttg cctcaatcat tgcccatcac ctcccggtaa tattccctgc cccgtatgtt
     1321 cgggtgctct atcggctttg ctgctttgtg ctcatcaagg tacgccacct tgtccagtcc
     1381 ggtttgtaat atcgatttca cactcccgta gttgcaggct tccacagcaa gtgccctttt
     1441 gcaggcactt tccacccgct ccggtgtgta ttgttttgag agtctgatta tccccaggca
     1501 actcctgaac ccctgctccg gatgtttttt gctctccatg atccgatgca tcatcgtttt
     1561 cgtatggagc ccgatcttgc caccccagtt tttgatcctc tccggtgtcc attcaaggta
     1621 ctgacggtgt tcatggggca tgtgactgtc ttctgttata tatttcccct ttttatacgt
     1681 ccgtatgtga gaggctacac gccggttatg gtgatatatc tctaccactc tgctgttgta
     1741 tttgatatca acatactggc ctatcaaggt ataggggacg ctgtaataac ttttctccac
     1801 ctctacgtga taatcgatat gcagttttgt cggctttttg taattatata tctcgaatct
     1861 ctccgcaggc agcggcctca atacaggctt ctctatttcc tcaaacagct cacgccggga
     1921 tttccctgtt actgccatcg gtcgactgtt aagcttctct acctcctccc gtatcgcttc
     1981 atccagttca cgaaggctaa aaaacgtcct gttcctcagg gccgcaagga ttctcctctg
     2041 tgcctgcaaa acggcatttt ccacttttgc cttgtcccgt ggtttgcctg ctctggcagg
     2101 taccaccgcc acatcgtaat gctctgccat gtctgcaaat gtcctgttta tctcagggtc
     2161 ataataacag gcgctcgtta ctccgctttt gagattgtca gggacgatgc atcggggaga
     2221 gccgccgtaa tattcaagtg cctgtatatt tgcctttatg aaattctcca ccttctgatc
     2281 agccacggta caggcaaagg tgtatgatga tgccccaagc gccgagacaa acagctccgt
     2341 ctccactgct tcccctgttt ctgggtttat atattgcggc ctgtctccag agaaatcctc
     2401 aaacatctta tctccggcct tgtgattgaa ccgcatcaca ggattcagct tctttgcata
     2461 gtgccggtac ttctcgtaaa accggctcct gctgtaaccg cctggattgc ctgctctgta
     2521 ttcttcatac aaaagctgaa gggtgactcc cttcttcttc agctcctttg tcagatactc
     2581 aaagtccggc attgctttat cgggcacggc tatctctcct gttgccggaa ataataggtt
     2641 gtataacgcc tcttcatcca gggatgacag ttcttcgtag ctgagcttta actgctctat
     2701 cttttttaca tatgtggatg ctgttgatga tgatatattg caggccccgg ctatctggtt
     2761 gattcctaac cccatcttca gccgtaatct gcacatttca aggatctttc tcatgcataa
     2821 cctcccgctt tttttcttca tttaccctcc tgcaactttt tggagggcat tttagtacaa
     2881 acagcaggaa attattaaag aaattcgcaa tcctatctgc tcgaacacta aatccggttt
     2941 tctcataaaa gtgttcgact atttccggat ttgctgttcg agctccatcg gattgggtgt
     3001 tcgacttaca caggattcgg tgttcgagtt gggccggatt ttacaaattg aatctgaaaa
     3061 aggaatgcgg gtagctacag acatggtctc atgtaaggat aagaaatttt atcttgctga
     3121 agactaccat cagaaacatg cccttcagcg gtactccgat ctttttcgcg aatacaggac
     3181 catatatccg gacatgcagt atttaatagc ctctaccgct gcggctcggg taaatggata
     3241 ccttgcggga tatggtgagt gtaataaact acaatccgaa atcgaaagcc ttggactttc
     3301 cataaaagga atgaaaagct tgctctccat aatatgccgt aaagaaacta tctttccatg
     3361 tgtagggtca gggagccttg agtaaataaa ctacgattga atgatactcc cgaacagaca
     3421 tctgtggctt ttatcatatg tagatgaatt gccgtttgaa atcagcaacg atactatcaa
     3481 aataaacatt gcacattcta gtctcctacc atcagttcat tcctattgat caattaaata
     3541 tatctccgtt catgctattt atccccgtaa at
//
LOCUS       DBNGSV010000041         6728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1044920, whole genome shotgun sequence.
ACCESSION   DBNGSV010000041 DBNGSV010000000
VERSION     DBNGSV010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1044920"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..956
                     /locus_tag="AC159U_01465"
     CDS             51..956
                     /locus_tag="AC159U_01465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3335756.1"
                     /translation="MANKIILGLILLMVVVPNAFISYADEFESVVIKTIKITDNVYML
                     QGRGGNIGVIAGEDGVLMIDDQFAPLSDKIKTAISNISDRPIRFVINTHWHRDHSEGN
                     ENFARAGTIIVAHENTRKRMNTEQFVKAFNKKVLPSPKEALPVVTFSSDIKFHINGEE
                     IEVIHIQNAHTDGNAVIYFKNSNVIHMGDTYFAGKYPFIDYSSGGGITGVVAGLQKVF
                     AMIDDDTKIIPGHGNLSNKQELGIYLDMLRDAKSGTEKFVKEGKTLQELLDSDLLKNH
                     EQRFGQGFLKRDKFLSIVYAGISGN"
     gene            1146..1373
                     /locus_tag="AC159U_01470"
     CDS             1146..1373
                     /locus_tag="AC159U_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335757.1"
                     /translation="MNKVILFFVLVTMMLFGAASNVFVTDVSADTSSAYSDSGKFDEH
                     EDAPSEEPEEEPEDGEDYPEDEGEDQPAEEG"
     gene            complement(1546..2073)
                     /locus_tag="AC159U_01475"
     CDS             complement(1546..2073)
                     /locus_tag="AC159U_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR3335758.1"
                     /translation="MDFKLMFFGFYNMNIDRIVISAVVFLMMIALIYLILLMVGSYGR
                     INKLHVKRTKYVIKLFSLFIIICYFIFLLVLWGINSQGILVLASTIFTVLGIALFATW
                     SVLSNIVSSIIIFFGCPYRIGDYVKIVDGDNTLEGKITDINFYNVILEDINANVITFP
                     NNIILQKAVIKNSTQ"
     gene            complement(2157..2318)
                     /locus_tag="AC159U_01480"
     CDS             complement(2157..2318)
                     /locus_tag="AC159U_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335759.1"
                     /translation="MKLICVYCGSLKENGVDTISYHICDKCIKTLKGSAKKKKPDKKK
                     NKRSKFFLS"
     gene            complement(2431..3087)
                     /locus_tag="AC159U_01485"
     CDS             complement(2431..3087)
                     /locus_tag="AC159U_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3335760.1"
                     /translation="MINLALIIKSNLLYELLDKKLRQEKEISISGRATGENELFQILS
                     ENEIDVVFIDSAVADLDYKALIEKLDEINSSLGLIVLFHNNDIDLFVNAIQCGIDGCL
                     NIKSSYADLVQSIKTVKEGGIWADNEILTSALKRFINKGDKTIKSLSSNLTKREYEVA
                     ELLYQGLSNKAIATKLYISEKTVKTHISHIFRKLGIKHRYELNPQILESFKQFKGIKS
                     "
     gene            complement(3264..3776)
                     /locus_tag="AC159U_01490"
     CDS             complement(3264..3776)
                     /locus_tag="AC159U_01490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR3335761.1"
                     /translation="MKSNKDNTNNNIYISDSFAGQGVFAAKKFYPGDLILTFSGKRVN
                     SDDPVHMTELGANLLQTGYKTYIMPESPAVFINHSCSPNAGIMKNRKLIAIKKINENE
                     EITFDYSTTMDEDLWTMQCLCNEPTCRHIISDFDTLPQSLKNQYISLNIVQKFIAHKY
                     KKHYRQMKFL"
     gene            4026..4601
                     /locus_tag="AC159U_01495"
     CDS             4026..4601
                     /locus_tag="AC159U_01495"
                     /inference="COORDINATES: protein motif:HMM:NF021759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HIR3335762.1"
                     /translation="MVKFTEDALFSIEHKTQILGIDFKVLTKTYLLDSWILQIKRLSK
                     NRLGQCNYIKKSIELNLDYLEVNSLQRFQNTLMHEVAHAMVYEKYRFDNKWKSIKPHG
                     KEWKEAAITIGAQPVRCPEFDGIKPKFNYWLECKVHGVIESWIRRPFWIYLNNRMSKS
                     VRRLLCPVCKEAGYKNYLQFLHLKNSVNRHI"
     gene            4716..5171
                     /locus_tag="AC159U_01500"
     CDS             4716..5171
                     /locus_tag="AC159U_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3335763.1"
                     /translation="MTRIRLVDPRRDFSSLQDRVNSLFDETFRGLSTQSYQDLETSDW
                     TPSVDISQDEKQFVFEVDLPGFDKKDINVDVRDSSLTISGEKKFDEKVQNENYIRVER
                     NYGKFSRSFTLPNTADQSKIDASYKNGVLKVTVAKKEEATPKKIDVKVS"
     gene            5454..6107
                     /locus_tag="AC159U_01505"
     CDS             5454..6107
                     /locus_tag="AC159U_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR3335764.1"
                     /translation="MRPVPFSYFHMPLLLLGIVAVLCIVTVYDPPPGRFSWLLEVGPG
                     LIGITVLIALHRRFPMSHMVYFFVFLHMLILIYGGYYTYAETPLGDWAKEVFGFSRNH
                     YDRVGHFALGVFPAFIIREGLLRRTPLQRDNWLYFITISIVLAVAAFWELLEWWVTLI
                     VASDIGIAFLGSQGDVWDAQWDMVLALIGAMIVLPILGPMHDRSMSKLFDIQSATKA"
     gene            complement(6213..6563)
                     /locus_tag="AC159U_01510"
     CDS             complement(6213..6563)
                     /locus_tag="AC159U_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335765.1"
                     /translation="MLKDRLDRKFSETAEEEWNLIREKIGNGIELPLPDSGRWFLATI
                     DGDSIKIESAKLNVRPIVINPPETITYEEFKQVVDVYPDILKGGVQMMSGKLELQKTM
                     ANFKYIFMLIYSLV"
BASE COUNT         2119 a         1141 c         1273 g         2195 t
ORIGIN      
        1 ggtgggacag ggtatattga taagaaacta aataaataat ggagttaata atggcaaaca
       61 aaatcatttt aggacttata ttattgatgg ttgttgttcc aaatgctttt atctcttatg
      121 cagatgaatt tgagagcgta gtaataaaaa caattaaaat aacagacaac gtatatatgc
      181 ttcagggaag gggtgggaat ataggagtaa tagcgggcga ggatggagtg cttatgattg
      241 atgaccagtt tgctccgctt agtgataaaa taaaaaccgc aatctcgaat atttccgata
      301 gacctataag gtttgtaata aacacccact ggcacagaga ccactctgag ggtaatgaaa
      361 attttgccag ggcaggcact attatagtag ctcatgaaaa taccagaaag agaatgaaca
      421 ccgagcagtt tgtaaaggca tttaacaaaa aggttctgcc ttcaccaaaa gaagcactgc
      481 cggttgttac tttttcatcc gatataaaat tccatataaa tggtgaagag atcgaggtta
      541 tacatattca aaacgcacac actgacggta atgccgttat ctactttaaa aattctaatg
      601 tcattcacat gggtgacacc tattttgcgg ggaagtatcc atttattgat tattcatcag
      661 ggggagggat tacgggtgtt gttgcaggtc tgcaaaaagt gtttgccatg atcgacgatg
      721 atacgaaaat tattcccggt catggaaatc tttctaataa acaggagctt ggcatatatc
      781 tggatatgtt aagagacgca aaatcaggca cagagaaatt tgtaaaagag ggtaaaaccc
      841 ttcaggaact tctggattcg gatctgctaa agaaccatga acagagattc ggacaggggt
      901 ttttaaaaag agataagttt ctttctattg tttatgctgg tattagtggc aactaaattg
      961 cagattattt ttaaccttaa aaataaatat taatgaaatc tgacaataga tttaaatttg
     1021 gtattaatac caggtcttat atttttcact aaatttctac acccaaatta ccactccggt
     1081 actattgtac acagcttcaa aaagaattat tttattgcaa aatttacaag gaggtaaaat
     1141 gacttatgaa taaagtcatt ttgttttttg tgttggttac aatgatgtta ttcggtgcag
     1201 catcaaatgt ttttgtaaca gatgtaagtg ctgatacaag cagtgcatat tctgattctg
     1261 gcaagtttga tgagcatgag gatgcacctt ctgaggagcc tgaagaagaa cctgaagatg
     1321 gtgaagatta tcctgaagac gaaggtgaag atcagccagc tgaggaagga tagtaaataa
     1381 aatttaatac taaagagtga tattattact ctttagtacc tctcataagt gggaagagtc
     1441 attatataat ttcataatac cccaaactct tcccacaaaa atctattcgg taagttaatc
     1501 ttactctcta ctacgctttt aacattagta attacgggca gtaatttact gggtactatt
     1561 ttttataact gccttttgca aaataatatt atttggaaat gtgatgacgt ttgcgttaat
     1621 atcttccaag attacattgt agaagtttat gtctgttatc ttgccttcga gcgtgttatc
     1681 accgtctaca atttttacat aatccccgat tctgtacgga catccaaaaa atattattat
     1741 gctcgagact atgttgctca gtactgacca tgtagcaaaa agagcaattc cgagcacagt
     1801 aaaaattgta gaagcaagta ccaggatccc ctgggaatta atgccccata gcaccagcag
     1861 aaaaatgaaa tagcagatga ttatgaaaag actgaacaat tttattacat attttgttct
     1921 cttaacatgc agcttgttta ttcttccata agatcccacc atcaataata ttagatagat
     1981 aagtgcaatc atcattagga aaactacggc tgatattact atcctgtcta tattcatatt
     2041 ataaaaacca aaaaacatta acttaaaatc caaaaatgta ctcaattatt ttatttacct
     2101 ctgcaaattt gtgttctaat aaaacaacca aagatccact taaaatatga tttattctaa
     2161 cttagaaaga acttcgaccg tttgtttttt ttcttatcag gtttcttttt ctttgcagag
     2221 cctttaagcg tttttataca cttatcacat atatgataag aaatggtatc aacaccattt
     2281 tcttttaagc tgccgcaata aacgcaaatt aatttcattc cttgcacctc ccgtaactgt
     2341 tatatgcgct taatgtaagg tttttacggg gctatactat gggacttttg gataatttaa
     2401 gaagtatgtt tgttgccgat cggtttcagt ctaggactta atacccttaa attgtttaaa
     2461 ggattccagg atttgtggat tcagttcata cctgtgctta atgcccagtt ttctgaatat
     2521 atgacttatg tgggttttta cggttttttc acttatatac aattttgtgg ctatagcttt
     2581 attactaagc ccctggtaca gcagttcggc aacctcatac tctctttttg taaggttgct
     2641 tgataacgac ttaatggttt tatcgccttt atttataaat ctcttaagcg ctgaggtcag
     2701 gatttcattg tctgcccaga tgcctccttc tttaactgtt ttgattgact gaactaaatc
     2761 tgcgtagctg gattttatgt tcaaacatcc atcaattccg cattgtattg catttacgaa
     2821 tagatcaata tcattgttgt ggaacagcac aattaacccc aggctggagt taatctcatc
     2881 gagcttctcg attaaggctt tgtaatcaag atcagcaact gctgaatcaa taaataccac
     2941 gtctatctca ttttcactga ggatttgaaa tagctcgttt tcacctgttg ccctaccgga
     3001 aatcgaaatt tccttttcct gccggagctt tttgtcgagc agctcataca gcaggttgga
     3061 ctttatgata agtgctaaat taatcaattt aatatcaata atatactcaa attattattt
     3121 ataaataata attcggtgta atatatatta tcaatattct ctggttttca atagataatt
     3181 ttattatgca ggtttatccc cggtgctggt ggtagaatct tcaatatttc aaagccaatt
     3241 cagcattaaa aataattgtg gttctataaa aacttcattt gtctgtagtg ctttttgtac
     3301 ttatgagcta taaatttctg aactatattt aatgaaatat attgattctt aaggctttgc
     3361 gggagtgtat caaaatcaga tatgatatgt ctgcatgtag gttcattgca cagacactgc
     3421 atagtccata aatcctcatc catcgtggtg gaataatcaa atgtaatttc ttcgttttcg
     3481 ttaatcttct ttatggcaat caattttctg tttttcatta ttcctgcatt tggactacag
     3541 ctgtgattaa taaaaaccgc aggcgactca ggcatgatgt atgttttgta tcctgtttgt
     3601 agcaaattag ctccaagttc tgtcatgtga acaggatcat cgctgttaac tctcttacca
     3661 ctgaaagtta atataaggtc gccgggataa aatttctttg ctgcaaatac cccttggcct
     3721 gcaaaagagt cagaaatata aatattatta tttgtattat ctttgtttga tttcattaaa
     3781 gtaatgaaat atatattgtt taatatatgg attttccgta aaaattaaat aatacgaata
     3841 gcgtatttta ggcttatata aatccaaaat attttagtcc ctggaattat gagactgtcc
     3901 gatcgttact cgatttaagc tgtatagttg tagtctggct aataccaaat tacataaaaa
     3961 gttctattga tttgctgtga tattaacgac atatttgtat tgaaaatggg agtgagaaat
     4021 taacaatggt taaatttaca gaagatgctc tgttttctat tgagcacaaa actcaaatac
     4081 tggggatcga ttttaaggta ctaacaaaaa cataccttct tgactcatgg attctgcaaa
     4141 taaaaagact ttcgaaaaac aggttgggtc agtgtaatta tattaaaaaa tctatagaac
     4201 taaatctcga ttatctcgaa gtaaacagcc tgcaaagatt tcaaaataca ttaatgcatg
     4261 aagttgcgca tgccatggta tacgaaaaat acaggttcga taataagtgg aaaagcatca
     4321 aacctcatgg aaaagaatgg aaggaggctg cgataaccat aggggctcag cccgtacgat
     4381 gtcctgaatt tgatgggatc aaacccaaat ttaattattg gttagagtgt aaagtgcatg
     4441 gagttataga aagctggata agaagaccct tttggattta tctcaataat cggatgagca
     4501 aatcagtacg caggctactg tgtcctgttt gtaaggaagc gggatataaa aactatctgc
     4561 aatttttaca cttaaaaaac agtgtaaaca gacacatcta atatctattc tgttttcaat
     4621 tttaaataaa aattcccatc ttgacagctt ttaaaccata attatttttt aggatataaa
     4681 gagatttaaa caataaattt taggagggaa ttgaaatgac tagaataaga ttagtcgacc
     4741 caaggagaga ttttagttct cttcaggaca gggtaaatag tttatttgat gaaacattta
     4801 gagggttatc aacacagagt tatcaggact tagagacaag cgactggaca cccagcgtgg
     4861 acatttctca ggacgaaaag cagtttgttt ttgaggtaga cctgccgggt tttgataaga
     4921 aggatataaa tgtagacgta agagacagca gccttacgat aagtggtgag aaaaagtttg
     4981 atgaaaaggt tcaaaatgaa aactatatca gggtagaaag aaactacggc aagtttagca
     5041 gatcttttac gctgccaaac actgcagatc aatctaagat cgatgcaagc tacaaaaacg
     5101 gggtgcttaa agtcaccgtg gctaaaaaag aagaagccac tccaaagaag atagacgtta
     5161 aggttagtta aagcgcaatt tgacgctgaa aataaaaaag gaagttgcat aggacgtaac
     5221 ttataaggat tagggtctat tctggcccta atccattaaa actccaaaaa ccaagaattt
     5281 tagtttctcc catcatggca tgacttaaaa taatctgcaa ctttaaaata acccaataat
     5341 caaagcctga ccctatatgc atttccataa tcaagataat ggatatataa tgttttttgt
     5401 ttacaaaatg taggggtaaa aaaaagtatg aatctgctta aattaatatt tacatgagac
     5461 ctgtcccatt ttcctatttc catatgccgc ttttgctgct gggtatagtg gcagtgctgt
     5521 gcatagtgac tgtatatgac cctccgccgg gaaggttcag ctggctgctg gaggttggcc
     5581 ctgggcttat tggtattacg gttctcattg cgttgcatag gcgctttccc atgtcacaca
     5641 tggtatattt tttcgttttt ctccatatgc ttatccttat atatggcggc tattacacct
     5701 atgcggaaac gccgcttggt gactgggcga aggaagtatt cggattcagc cgcaatcact
     5761 atgaccgtgt aggtcatttt gctctgggtg tgtttcccgc ttttatcata agagagggtc
     5821 tgctgcggag gactccgctg caaagagata actggcttta ctttattaca ataagcattg
     5881 ttcttgccgt ggcggctttt tgggaattac tggaatggtg ggtaactctt atagttgcat
     5941 cagatatagg tatagcgttt ctgggttctc agggcgatgt atgggatgcg cagtgggata
     6001 tggtcttggc acttataggc gctatgattg tgcttcctat tttgggccca atgcatgata
     6061 ggtctatgtc aaagctcttt gatatccagt cagccactaa agcttgaaag tgtatcggta
     6121 tcagaatcaa caagattgtc ccgtattatt tcggcgggaa atatcggtat gattagctgt
     6181 aaatctgaat gttctggact ggacttgcgt ttctaaacca aactataaat cagcataaat
     6241 atatacttga aattggccat ggtcttttgg agctcgagct tgccgctcat catctgtacg
     6301 cctcccttca aaatgtcagg gtagacatct actacttgtt tgaattcttc ataagtaatc
     6361 gtttcaggag gattaattac aattggtctt acatttagct tagcgctttc aattttgatg
     6421 ctgtctccat cgatggtagc taggaaccat ctgccggagt ctggcagtgg aagttcgata
     6481 ccatttccaa ttttctctct gataagattc cattcttctt cagcagtttc actaaatttt
     6541 ctgtccagtc tgtctttaag cattgttgtt gcctccaaaa gtaatcttac aagtattaca
     6601 acttaataca gctttgtcaa agtaatatga tggttgaata tattatagta caacacaaac
     6661 atgctgctat gtgatctcag gcttcaaaat ctttgtttca cccttataat ttttcttgaa
     6721 taatgccg
//
LOCUS       DBNGSV010000042         5505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_629206, whole genome shotgun sequence.
ACCESSION   DBNGSV010000042 DBNGSV010000000
VERSION     DBNGSV010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_629206"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..965
                     /locus_tag="AC159U_01515"
     CDS             417..965
                     /locus_tag="AC159U_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3335766.1"
                     /translation="MALEDSELIKRVDTELNMKNDIEDIYQMSADILLKLESINMVMI
                     YIVDPSSDQAVIVSHKNVPESYLTRAGRIDRPRGITWKLILENDILNIKNSQTDDTIG
                     PAGKALGKKGVYGVPIALSGGSKEARGVIWLWSDDEISFDSDQHRLALYVAQKVGLAI
                     TWSEKYAERREEWKNKFKVDTP"
     gene            complement(966..1292)
                     /locus_tag="AC159U_01520"
     CDS             complement(966..1292)
                     /locus_tag="AC159U_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3335767.1"
                     /translation="MNKPNIYTNLPHNLSEELFETLISNKNIKIQRIVSPKSFKSPKN
                     KWFNQNRDEFVILLQGSAELVFKNNGEKVYLKPGDYINIPCNREHRVFSTDKQVQTIW
                     LAIRYK"
     gene            complement(1311..1670)
                     /locus_tag="AC159U_01525"
     CDS             complement(1311..1670)
                     /locus_tag="AC159U_01525"
                     /inference="COORDINATES: protein motif:HMM:NF023390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIAA family protein"
                     /protein_id="HIR3335768.1"
                     /translation="MLQLKSLDAGHVVGCVVDGKINSDDIDTIWKEIDRKFKGHEKLS
                     IYVEVKNYGGISLDAFFEDLKRGYKHFSNFSKKAVVTDKNWIKKMTPVVNKIFPNIDV
                     KCFSFEDKEKAIEWVQN"
     gene            complement(1920..2570)
                     /locus_tag="AC159U_01530"
     CDS             complement(1920..2570)
                     /locus_tag="AC159U_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024063.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HIR3335769.1"
                     /translation="MSIIYEIAEYEKIVISLTGFTLSAIFIILGILIYYYKCYNLIAG
                     YNLSSENIKKQYDIEGLSKHIGHGLIVLGVLLFVSTILILTGLITLFTITAFIVLFMV
                     LIIILGSRKFMPYTQNLIKESPSDAKHHFLHWILPEKVFRALEKGSRQWLQQCRHCGY
                     KQDYWEAGNIRYKAIGEPTKLLWCENCKKFRFHKIRKKSVLEAESIDSQNIKINSF"
     gene            complement(2592..3083)
                     /locus_tag="AC159U_01535"
     CDS             complement(2592..3083)
                     /locus_tag="AC159U_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3335770.1"
                     /translation="MKILKYMISIYLLIMFTSLGSLNAEQDDNAVIKANSKFYVALNE
                     MFKGNVEPMKEAWSHSDDVTYLPPTGGIKSGWKEVLSEWQAQADQKLGGTVKSENIRI
                     SMGKDIAVVVNYEKGSNVDSDGNHVEISIRATNTFRKENGKWKMIGHHTDLLPFLQKQ
                     DSH"
     gene            3222..3770
                     /locus_tag="AC159U_01540"
     CDS             3222..3770
                     /locus_tag="AC159U_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR3335771.1"
                     /translation="MKSINILLTILFLTLFSANISVANSAAEINAKAELSLKHLYNNS
                     PGAKTLANDAKAILVFSEIVKAGLIVGGQLGDGVLLKNGEPTAYYRSVAASYGLQAGV
                     QKFGYALFFMDNDSLDYLEKSKGWEIGSGPSVVVVDEGFGKKLSSTTLKKGVYAYIFD
                     QKGLMAGIGIEGSKITKITPDN"
     gene            complement(3771..4250)
                     /locus_tag="AC159U_01545"
     CDS             complement(3771..4250)
                     /locus_tag="AC159U_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335772.1"
                     /translation="MIFNNSFLTAIALLCFLTLYISCNNDTVASEFPECFVGTYLNEE
                     GSGTKSIWNLSDDGTFNGQSSTEQVFNFTGQLGTWRSTGINTAEILMIDFSFDDDGNF
                     ANTARIDIEIEFKGENCEETEGSFQLRFFEDDEDPLDVSTDTGDAIEDSFTGRKVII"
     gene            complement(4326..5459)
                     /locus_tag="AC159U_01550"
     CDS             complement(4326..5459)
                     /locus_tag="AC159U_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR3335773.1"
                     /translation="MFGKQIKLFSLLGFDVAIDTSWLILAFLITWTLAQGYFPHYYKD
                     FTAATYWYMGICGAIGLFFSIIFHELSHSVVARKYGIPMKGITLFIFGGVAHMEEEPP
                     SAKSEFLMAIAGPISSIILAGFFYLISLYSIATGYPEPVSAVLSYLAFINTILAVFNL
                     LPGFPLDGGRVLRSALWYWKNNIKWATKVASQIGTAIGTFFIILGILAIITGNFIGGL
                     WWALIGMFLRNASSISYQHLTLRNAFEGEKVKRFMKQDPITVPSSISIHDLVENYFYY
                     YHYKMFPVSDNSKLLGCVTTRDVKNIPKENWDRIRVKEITNICSPENTIDINTEAINA
                     LTLMNITGNSRLMVLDNGNLAGILTLKDMMKFLSVKIDLENND"
BASE COUNT         1753 a          933 c          955 g         1864 t
ORIGIN      
        1 cacttccaat acaaacattt gtattagctt gcttatctta ctatttgata gttttgtatt
       61 cttaagtaac ttcttttgca tagtttcagc ttagtatact ttgctgtcct atgcgagctc
      121 ctaaaataat tattattgtt aaattaaaaa tgtgtcttaa atgtttctat actagaatat
      181 cactatattt agtgattagg tgtataatat attgagtaat tattacattt gatttgatat
      241 ttgagcggtt ttaatattat tcttagatta agattgtatt atgatatatt tttaggttaa
      301 aaaaaaatat cttcaaaaac cctctaaaat gtgatattaa tctgtttgtc ttgattacaa
      361 agatattcag tggataaggg gataatatat aatttaattt aaggagtagg gatgaaatgg
      421 cactagaaga tagtgaatta ataaaaaggg tggataccga actcaatatg aaaaatgata
      481 ttgaggatat ttaccagatg tctgccgaca tacttttaaa actcgaaagt attaatatgg
      541 tgatgatata tattgtcgat ccgtcaagtg atcaggcagt gatagttagt cataagaatg
      601 tacctgaaag ctacctcaca agagctggaa ggattgacag acctagaggt attacatgga
      661 agctgatact ggaaaatgac atactgaata tcaagaattc ccaaactgac gatactatag
      721 ggcctgctgg taaagcattg ggtaaaaaag gtgtgtatgg agttcctata gcccttagcg
      781 gcggctcgaa agaggcaaga ggtgttattt ggctttggag tgatgatgag atttcatttg
      841 atagtgatca gcacagattg gctctatatg ttgctcagaa ggtaggttta gcaattacct
      901 ggagtgaaaa atatgctgaa agaagagagg aatggaagaa taagttcaag gtcgatacac
      961 catagttatt tataacgtat agctaaccat atagtttgga cttgcttgtc ggttgaaaat
     1021 actctgtgct ccctattgca tggaatatta atataatcac ctggtttcaa atatactttt
     1081 tccccattat ttttgaatac cagctcagcg cttccttgca gtaatatcac aaactcatct
     1141 ctattctgat taaaccactt atttttaggt gacttaaaac ttttgggtga gactattctt
     1201 tgtatcttga tatttttatt tgaaataagg gtttcaaata gttcttcaga caaattgtga
     1261 ggcagatttg tataaatatt tggtttgttc atgacaatat ctggtaatat tcagttttga
     1321 acccactcta tggctttctc tttatcttca aaagaaaaac attttacatc tatattaggg
     1381 aatattttat tcacaacagg agtcattttc ttaatccagt tcttatcagt aactactgct
     1441 ttttttgaaa aattactgaa atgcttatag ccccgtttta aatcttcaaa aaatgcgtct
     1501 aaagatattc ctccataatt ttttacttca acatatatgg agagtttttc atggccctta
     1561 aactttctgt ctatctcttt ccatattgta tcaatatcat ctgaatttat ctttccatcc
     1621 actacacatc ctactacatg accggcatct aatgatttta gttgcagcat ctgttacacc
     1681 tcctgataaa atattagaat atttatttct atgtgtttaa tttctttgct ctagttctta
     1741 tataattatt agttttaaat tatcaagtca atatagaaga taaacattaa tatgatcaca
     1801 gtatctagac ttcatgagac atatggaaaa acagttgcac ttagagggct ggattttcaa
     1861 atatccatcg gtggaattgt actatgtttg gttctaatag agagattaag tcgactcttc
     1921 taaaaactat ttatctttat attctggcta tctatgctct cggcttcaag gacagatttt
     1981 ttcctgattt tatgaaacct gaatttcttg cagttctcgc accaaagaag cttagttggt
     2041 tcacctatag ctttatatct gatgtttcct gcttcccagt agtcttgttt atatccacag
     2101 tgtctgcatt gctgaagcca ctgtctggat cctttttcca gggctctgaa tactttttct
     2161 ggcagtatcc aatgcaggaa atggtgcttg gcatcagatg gtgattcttt tatgagattt
     2221 tgcgtataag gcataaactt tcttgaacca agaataatta taagcaccat aaataagact
     2281 ataaatgctg tgattgtaaa aagtgtaatt aaaccggtaa gaattaatat tgtggaaaca
     2341 aataatagca ccccaagaac tataaggcca tgacctatat gtttggataa cccctcaata
     2401 tcgtattgtt ttttaatatt ttcagaggag agattataac cggctatgag gttatagcat
     2461 ttgtaataat atattaaaat tcctagtatg atgaatatag cagataatgt gaatccggtt
     2521 aatgatatta ctatcttttc atactcagct atttcataga taatgctcat ggtacataat
     2581 attatatacc tttaatgaga atcctgcttt tgtaaaaacg gcagtaaatc cgtatggtgt
     2641 ccaatcatct tccattttcc attttctttt ctgaatgtgt ttgttgctct tattgatatc
     2701 tctacatgat taccatcaga atcaacattt gaaccttttt cataatttac aactacggca
     2761 atatcttttc ccatggatat tctaatattt tctgacttta ctgtaccacc cagcttttgg
     2821 tctgcctgtg cctgccattc agaaagtact tctttccatc ctgatttaat accacctgta
     2881 ggtggcaaat atgtaacatc atctgaatga gaccatgcct ctttcatagg ctcaacattt
     2941 cccttaaaca tttcatttag ggcaacgtaa aactttgaat ttgcctttat aactgcatta
     3001 tcatcttgtt ctgcatttaa tgaccctaat gatgtaaaca taatcagtaa gtatatggaa
     3061 atcatatatt ttagtatttt catttttaga gcactccttg ttatctgatg taatgtgaat
     3121 aatttaatga attatataat catttgcaat gtatttattg aatgtatact ttagacagtg
     3181 tagtaattta aaatattata aagaataaaa taggagaaaa tatgaaatct ataaatatat
     3241 tattaactat attatttcta actttatttt cagcaaatat ttctgttgct aatagcgcag
     3301 ccgagattaa tgctaaagcc gagttgtcat taaagcattt atataacaac tcgccaggtg
     3361 caaaaacact tgctaatgat gcaaaagcaa tattagtctt ttcagaaata gtaaaggcag
     3421 gcctgatagt aggagggcaa ctgggtgatg gagttcttct taaaaacgga gaacctacag
     3481 catattacag atcagttgcg gcatcgtacg gactgcaggc tggtgtacag aaattcggtt
     3541 atgcgctatt ctttatggat aatgactcat tggattacct cgaaaagagc aaaggatggg
     3601 agattggttc agggccgagt gtggttgtag tagatgaagg atttggcaaa aagctaagct
     3661 caacaacact aaaaaaagga gtctatgcct atatatttga ccaaaaaggt cttatggcag
     3721 gtataggaat agaaggctct aagatcacaa aaattacacc ggataattga ttatattatt
     3781 accttcctac ctgtaaagga atcctctata gcatccccgg tgtcagttga aacatcaagc
     3841 gggtcttcat cgtcttcgaa aaacctcagt tgaaaactgc cctctgtttc ttcacagttt
     3901 tcacccttaa actcaatctc tatatcaatg cgtgcagtat ttgcaaaatt tccatcgtca
     3961 tcaaatgaga aatctatcat taatatttcg gcagtattga ttccggtact cctccaggtt
     4021 cctaactgac ccgtaaagtt aaaaacctgt tcagtagagc tctgtccgtt aaatgtgcca
     4081 tcatcagaca agttccatat gctcttggtt ccgcttcctt cttcatttag ataagtacct
     4141 acaaaacatt cagggaactc ggatgccaca gtatcattat tacaggaaat atataaagtc
     4201 agaaaacata gtagggctat agctgttaag aaagagttat taaaaatcat tatgattcct
     4261 ccggtaattt ttttataact aagataagct ttaaaatcag attgttcaat ctaataaaaa
     4321 tctgatcagt cattattttc taaatctatt ttaacagata agaacttcat catatctttt
     4381 aatgttaaga ttcccgcaag attaccatta tctaaaacca tgagtctgct gttgccggta
     4441 atattcatga gggtaagggc attaattgct tcagtgttta tgtcaattgt gttttcgggg
     4501 ctgcaaatat ttgtaatctc ttttaccctg attctgtccc aattttcttt tggaatattc
     4561 tttacgtcgc gtgtagttac acacccaaga agtttagaat tgtctgacac cggaaacatt
     4621 ttataatgat aataatagaa atagttttcg actaaatcat gaatagagat cgatgaaggt
     4681 acggttatag ggtcttgttt catgaaacgt tttacctttt caccttcaaa agcgttacgt
     4741 agtgtaagat gttgataaga tatgcttgaa gcgtttctta aaaacatgcc gataagtgcc
     4801 caccaaagac ctccaataaa gtttccggta attatggcta gaataccaag tatgatgaaa
     4861 aaagtaccta ttgccgtacc tatttgtgaa gcgactttgg ttgcccattt aatattgttc
     4921 ttccagtacc agagggcaga acgaagcacc ctgccaccat ccagcggaaa tccgggcagt
     4981 aagttaaaga cggctaaaat tgtatttata aaagccagat aactaagtac cgctgataca
     5041 ggctcaggat aacctgttgc tatggagtat aaagagataa ggtaaaaaaa cccagccagg
     5101 ataatgctcg atatgggccc ggcaatagcc atcaggaatt ctgactttgc acttggaggt
     5161 tcttcttcca tatgagcaac tcctccaaaa ataaacaggg tgataccttt catagggatt
     5221 ccatattttc tggctactac ggaatggctt aactcatgga atatgataga gaaaaacagt
     5281 ccgatagcac cacaaattcc catataccaa taagttgctg ctgtgaaatc tttataataa
     5341 tgagggaaat acccctgtgc cagggtccat gttataagaa atgccagaat gagccagctt
     5401 gtatcgattg caacatcaaa tcccaataga ctgaataatt taatttgctt tccaaacata
     5461 tctttaatgg tatttaatta gggcattaga catctggcgg agtag
//
LOCUS       DBNGSV010000043        10028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1184138, whole genome shotgun sequence.
ACCESSION   DBNGSV010000043 DBNGSV010000000
VERSION     DBNGSV010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1184138"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..934
                     /locus_tag="AC159U_01555"
     CDS             188..934
                     /locus_tag="AC159U_01555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3335774.1"
                     /translation="MDKESLDKAIELVENANIVAVLTGAGISEESGIPTFRDEGGLWK
                     EHRPQDIATPEAFARDPQFVWEWYDLRRQTIIEAEPNPGHFALVELENKKEGFTLITQ
                     NIDGLHKKAGNKSMIEMHGNIWQMRCTKCGHIYHDYEVPLKQIPPSCEKCGEMTRPNV
                     VWFGEIIPMSAIDACVIAIDKCDVMLIIGTSGVVEPAASMGLMAMRSGKKVIEINLDT
                     TPGTGMYDISLKGKSGEILPEFYKHTTEYN"
     gene            complement(943..2607)
                     /locus_tag="AC159U_01560"
     CDS             complement(943..2607)
                     /locus_tag="AC159U_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3335775.1"
                     /translation="MNAALIAIIVLTLYFLGYRYYSHFIAKKIFRLSDDEVTPAHELK
                     DGVDYVPSNKHVLFGHHFASIAGAAPIIGPAIAVFWGWVPAIIWVCLGTIFMGAVHDF
                     SALVVSARNKGRSVGDLAGILVNPRARTLFLLIVYFLIFFVIAVFAFAIAVLFTKFPT
                     SVLPVNFQIVVAVAIGFLFYKRGVPILIPSIVALISLYFMIWLGTSVPITIPAVMGSQ
                     VVTWILILMVYSFIASILPVWTLLQPRDYINSHQLIVGLGILIIGLFVAHPQMQAPAF
                     NSNAEGGIPIFPFIFVTIACGAISGFHGLVSSGTTSKQLNRMTDARQIGYGGMIGEGT
                     LAMIATLAVAAGIQRSDWLVHYDSWQSASSGGISNFVIGSSSFLKAIHIPELFGTTLI
                     SVIVISFAATSLDTATRIQRLILGELGTAYKIEPLKNRYVGALLAVVPALLLALLAQA
                     PGKGPGSGGFLLWPLFGATNQLVGGLTLLVASIYLWKTGRPVIYTLVPMVFLILITIG
                     SMILNFQAFVNNPLLLVLSAIVLALAVWLLLEAFIMYKKSRNKGPT"
     gene            complement(2665..3594)
                     /gene="pdxA"
                     /locus_tag="AC159U_01565"
     CDS             complement(2665..3594)
                     /gene="pdxA"
                     /locus_tag="AC159U_01565"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3335776.1"
                     /translation="MGDPNGIGPEIILKALCCAEVQSICRPIVYGSKDVLDKAKDIVA
                     MDAVYQMEQCGDFALDNLNPGKVSKESGEISLVCIETAVTHIKESKIDFIVTAPITKE
                     AIHLAGSKFPGHTEMLAELSGADHFAMMFEGDKFKVVLATIHEALKDVPGILNKDDLF
                     YKLVMANDALKNYFGIERPRIVVCGLNPHAGEAGAFGTEEQDIIIPAIDRARLSGMDI
                     EGPLPADTLFYHAAGGRWDIVVAMYHDQGLIPFKMLNFEKGVNVTIGLPFIRTSPDHG
                     TAFDIAWKGEANPSSMIQAIKVAVNMYLNSRHQ"
     gene            complement(3679..4437)
                     /locus_tag="AC159U_01570"
     CDS             complement(3679..4437)
                     /locus_tag="AC159U_01570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3335777.1"
                     /translation="MLIDSHAHLGSLENIDQAVQNALDNSVSNIINMSSDLPSAHETI
                     EFINKYPGVYGAVGIHPHAAKTYSTEVFAELSELTNTEKIVAVGETGLDYFYDFSPRD
                     IQKKSLQNHINLGIETGLPIVLHVRDCEDDMKTMLKDNSCTDLKGVIHCFTGDYESAE
                     LYIELGFYISFSGILTFKRSEELREVAKKLPLDKLLVETDSPYLAPVPYRGKKNEPAY
                     VKHVAETLAQVREKSFEEIAQITTNNCKELFGIS"
     gene            complement(4448..4921)
                     /gene="nrdR"
                     /locus_tag="AC159U_01575"
     CDS             complement(4448..4921)
                     /gene="nrdR"
                     /locus_tag="AC159U_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR3335778.1"
                     /translation="MKCPFCSDLENKVMDSRLTNDGLAVRRRRECLACNKRFTTYERV
                     EEVNFLVVKKDGSKEVFDKSKLIHGMLKACEKRPISVDTIEGFINNLEREFQERGDKE
                     ISSTEIGEKVIRKLYEVDEVAYVRFASVYRSFKDVNQFMDELKDLLKDKDDGSDS"
     gene            complement(4925..6169)
                     /locus_tag="AC159U_01580"
     CDS             complement(4925..6169)
                     /locus_tag="AC159U_01580"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3335779.1"
                     /translation="MQSIMDTDPEIYDVIKSEIARQEWKLELIASENYVSRAVMEALG
                     TVLTNKYAEGLPGKRYYGGCEFVDIAETLAIERAKKLFGAHAVNVQPHSGAQANMAVY
                     FAAVQPGDTVLGMNLAHGGHLTHGSPVNFSGRLYNIVAYGVKKEDSLIDYDEVRSLAQ
                     KHNPKLIVVGWSAYPRKIDFKIFREIADEVGALLMADIAHPSGLVATGLYPNPVPHCH
                     FVTTTTHKTLRGPRGGMVMTDEEHAKVINSRVFPGTQGGPLMHVIAAKAVAFLEALQP
                     EFKDYQKQIVKNAQTMAERLQEHGFNILTGGTDTHLVLVDLTNKELTGKETEEALEKA
                     GITVNKNAVPFDPNPPMITSGIRIGTPAITTRGMKESEMITISDMIARAVENRDSEGE
                     LKKIEGQVKELCTSFPIYKELL"
     gene            6380..7078
                     /locus_tag="AC159U_01585"
     CDS             6380..7078
                     /locus_tag="AC159U_01585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3335780.1"
                     /translation="MIKNTKKAIFFDFGDTLASTDPPYIFRIASGFRRAGYDISDIDF
                     EHAYLRADFEIFKTHQSQGSMNSSQYNSLFFPLLCKALDIKEDPDDVRANIRKNTTPR
                     RFYRNALPGTQKLLNYLKQKGYILGIISNNDGYTNKKCKDVDIHRFFDIIADSTNIGY
                     VKPDPRIFHHVLDELGLDPEDCLHVGDLYGADVLGAKNAGIEVVWLNYRGINIMNDTE
                     TVQIKELTELIELL"
     gene            7127..9862
                     /gene="uvrA"
                     /locus_tag="AC159U_01590"
     CDS             7127..9862
                     /gene="uvrA"
                     /locus_tag="AC159U_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3335781.1"
                     /translation="MEKFIHVKGARLNNLKNIDVNIPLHKFTVVTGLSGSGKSSLALD
                     TIYTEGLRRYIESLSTYARQFLDRVEKPDIDEIKGIPPAIAVESRNSIKNSRSTVGTI
                     TEIYDYLRLLFAKVGRLICPNCRIEVKKHSPQSIAEFVLKDFCEQVLIITIKTTIDDR
                     MNPDIPLSKGFTKILRDGHIHDIEELDGLTDKDEIVVDRLRVNEDNKSRLIDSTESGF
                     IQSNELIFNIGKDSIFRFTKKLQCPKCTKEFSEPTPQMFSFNSPSGACRECSGFGNTL
                     DLDPDLVVPDPDKSLYEGAIEPFTKPSLDHARTRLMHFAKKSGINTNVPYKDLSDEQK
                     ELIFSGGEGYRGVRGYFKRLEGKNYKLHVRVFLSKYRSAYTCSVCHGTRIVPEALWFK
                     IGGKNISEISGMPADELATFINKLKLSDYESEIASEILKQIKSRVGFLLKVGLDYLTL
                     SRLAKTLSGGEAQRVNLSCQLGGGLTETLYILDEPSIGLHQRDIDQLIAIIKELTQRN
                     NTVILVEHDMDMIRASNYIVELGPKSGEHGGQLVFEGSKSKFTKNSFESITKSYYLGE
                     KEIEVPKKRRKGNGKYITIYGAKENNLKSIDVSFPLGTFICVTGVSGSGKSSLLNDIL
                     YSALARKFNSDIERVGNYKSIKGTEHISNIYLLDQKPIGKSSRSNPATYLKIYDDIRK
                     IFADTWTARSKGLKPSHFSFNVQGGRCDKCEGEGQETVEMLFLADVHITCEECKGKRF
                     KDEVLSIQYKKKNIDEVLQLTIDQAVSFFRETPKIVQKLNVLSDVGLGYLRLGQSSAT
                     LSGGEAQRIKIAKELSGKKGKDILYILDEPTVGLHIDDINRLLKVLNKLVDSGNTVIL
                     IEHNLDVIKSADHIIDLGPEGGQSGGNIVACGTPEQITAIDKSYTGKYLKPLLSN"
BASE COUNT         3130 a         2043 c         1939 g         2916 t
ORIGIN      
        1 aacagtactt cgcctcttag tcctgatatg aatttatcta agacattttc tttaatagat
       61 acagtatcat tatcaagtgt agtgattgtg agttttgcca tttaacatct ccgtttgttt
      121 taggtgagat cggtatatat actattattt taacatcaat atttaagctg tataaacatt
      181 ttatgatatg gataaggaga gcttagataa agctattgag ttagttgaaa atgcaaacat
      241 tgtagcagtc ctcactggtg cagggatatc agaagagagc ggcattccga cattcaggga
      301 tgaaggtgga ctatggaaag agcacagacc gcaggacatt gccacacccg aagcttttgc
      361 aagagacccg cagtttgtat gggagtggta tgatctgagg cgacagacga ttatagaagc
      421 agaacctaac ccgggccatt tcgctctcgt tgagctagaa aataaaaaag agggatttac
      481 gctcattact caaaatatag acgggcttca caaaaaggcc ggaaacaaaa gcatgatcga
      541 gatgcatggc aacatatggc aaatgcggtg cacaaagtgc ggacatatct atcatgacta
      601 cgaagttcct ttgaaacaga ttccgccctc atgtgaaaaa tgtggagaga tgacaagacc
      661 aaatgttgtc tggtttggtg aaattattcc catgtctgca attgacgcat gtgtaatagc
      721 aatagataaa tgtgacgtaa tgcttatcat cggcacatct ggtgtagtag aaccagcagc
      781 atcaatggga cttatggcaa tgaggtctgg aaaaaaagtg attgagatta acctcgatac
      841 cacaccgggc acgggcatgt atgatatttc actaaaagga aaatcgggtg aaattcttcc
      901 tgagttttat aaacatacaa ctgaatataa ttaacctctc agtcaagtag ggcccttatt
      961 tctggatttc ttgtacatta taaatgcttc gagtagaagc cataccgcaa gagccagaac
     1021 tatagcggag agtacaagca acagcggatt attaacaaat gcctgaaaat ttaaaatcat
     1081 agatcctata gtaataagta ttaaaaacac catgggtacg agtgtataaa taacaggtct
     1141 tccggttttc cagagataga tactcgctac caggagagta agccccccta caagctgatt
     1201 agtggcacca aaaagaggcc ataataaaaa ccctcctgag cccggacctt taccaggtgc
     1261 ctgagctaaa agggctaaaa gcagagccgg tactacagca agtaatgctc ccacgtatct
     1321 gttttttaga ggctctattt tataagcagt tcctaattca ccaagaataa gcctttgtat
     1381 tcttgttgca gtatcaaggg aagttgctgc aaaacttatt actattacac ttataagagt
     1441 ggtgccaaat agctcgggaa tatgaatggc ttttaaaaaa ctgctcgaac caataacgaa
     1501 atttgatata cctccgctcg aggccgactg ccaggaatca taatgaacaa gccagtccga
     1561 tctctgtatc cctgctgcaa cagctagagt agctatcatg gcaagagtgc cctcgcctat
     1621 catacccccg tatcctatct gccttgcatc ggtcatcctg tttaactgtt ttgaagttgt
     1681 accactcgat acaagcccgt gaaatccgct tattgctccg caggcaatag ttacaaaaat
     1741 gaaaggaaat attggtattc caccctccgc atttgaattg aatgccgggg cctgcatctg
     1801 aggatgcgct acaaatagac caattattaa tattccaagt cctactatga gctggtggct
     1861 gtttatatag tctcttggct gcaatagtgt ccacacggga aggatggaag ctataaaaga
     1921 gtagaccatt agtataagta tccatgtaac aacctgtgaa cccatcaccg caggaattgt
     1981 tatggggaca ctagttccta gccagatcat gaaatacagg gatataagcg ctacaataga
     2041 cgggatgagt attggcactc cccttttata aaaaagaaac ccaatagcta cagcaaccac
     2101 tatctgaaag tttaccggca gtacacttgt agggaacttt gtaaaaagca cggcaattgc
     2161 aaaagcaaat actgcaatta caaaaaagat gagaaaatag actataagca ggaatagtgt
     2221 ccttgcacgt gggtttacta aaataccggc aagatcaccc actgacctcc ccttatttct
     2281 tgctgaaacg acaagagcac tgaagtcatg caccgcaccc ataaatattg ttccaagaca
     2341 aacccagata attgcaggta cccaacccca gaaaaccgca atcgcaggtc ctattattgg
     2401 agctgctccg gctatagatg caaaatggtg cccaaatagt acgtgcttat tgcttggtac
     2461 atagtctact ccgtctttta gctcatgtgc gggcgttact tcatcatcag aaagtctaaa
     2521 tatttttttt gctataaaat gtgaataata tctatatcct aaaaaatata gagttaatac
     2581 aataatagct ataagtgctg cgttcatttt ttcataccat gtgattaata aatcatgaat
     2641 tatataacac aataagaaat aagactattg gtgtctggaa tttaggtaca tattaaccgc
     2701 caccttaata gcttgtatca tacttgatgg atttgcttct cccttccaag caatatcaaa
     2761 cgccgtgccg tgatccggag aggttcttat aaatggcaag ccaattgtca cattcactcc
     2821 cttttcaaag ttaagcattt taaacggaat aagcccctga tcatgataca tagcaactac
     2881 aatgtcccac ctgccgcctg ctgcatgata aaacagagtg tctgcaggaa gcgggccttc
     2941 aatatccatt ccgctgagcc ttgccctgtc tattgcagga ataataatat cctgttcttc
     3001 tgttccaaaa gcacctgctt cgccagcatg tgggtttaga ccgcatacta caatcctggg
     3061 cctttctatt ccaaaatagt tcttaagagc atcgtttgcc attactaatt tataaaaaag
     3121 atcatcttta ttaagaatcc ccggaacatc tttaagcgct tcatgaatgg ttgcaagaac
     3181 taccttgaat ttatcccctt caaacatcat ggcaaaatga tcggctccgc taagctcagc
     3241 aagcatctcg gtatggccgg ggaattttga gccagccaga tgaatcgctt cttttgtaat
     3301 tggtgcagtt acaataaaat cgatcttact ctctttaata tgagtaaccg ccgtttcaat
     3361 acatacaagt gaaatttccc cggattcttt actcactttt ccgggattaa gattatccag
     3421 agcaaaatcc ccgcattgtt ccatttgata tactgcgtcc attgcaacta tatcttttgc
     3481 cttatcaagg acatccttgc ttccataaac aataggacgg caaatgctct gcacctcggc
     3541 acagcaaagg gcttttaata taatctccgg gcctatacca tttggatcgc ccatagtaat
     3601 tcctacagtt ggtttatata cgtcctgcat agaaagaata ataaagtgaa tagaagataa
     3661 ataaagagtt tagtgaattt aggaaatacc gaacagttct ttacagttat ttgtggttat
     3721 ttgagcaatt tcttcaaatg atttttctct tacttgagca agagtctctg ctacatgttt
     3781 tacataggca ggctcattct tttttcctct gtatggaacc ggggcaaggt atggtgaatc
     3841 ggtttctaca agcaacttat caagcggcag tttctttgcc acttccctaa gctcttctga
     3901 tcttttgaat gtaagaattc ctgaaaatga aatatagaat ccaagctcta tataaagttc
     3961 tgctgattca taatctcctg taaagcagtg aataacacct tttagatctg tgcatgaatt
     4021 atccttaagc atggtcttca tatcatcttc acagtccctt acgtgtagaa ctattggaag
     4081 tccagtttca atgcctagat ttatatgatt ttgcagtgat tttttttgaa tatcccttgg
     4141 agaaaaatcg tagaagtaat caagccctgt ttctccgact gcaacaattt tttctgtgtt
     4201 agttaactcc gatagttcag caaagacttc cgtagaataa gttttagctg catgaggatg
     4261 aataccaact gctccatata ctcccgggta tttatttata aactctattg tctcatgagc
     4321 cgacggcaga tcggaagaca tatttattat attagataca gaattatcaa gcgcattttg
     4381 cacggcctga tcgatatttt ctaaagagcc cagatgggca tgggaatcta taagcataaa
     4441 ttagttttta ggaatccgat ccgtcgtctt tatcttttag caggtcctta agctcatcca
     4501 taaactgatt tacgtcttta aatgacctat acaccgaggc aaagcgcaca taagcaacct
     4561 catcaacctc atataacttt cttattactt tctctccaat ttcagtgctg cttatttcct
     4621 tgtcacctct ttcctgaaac tcacgctcga gattatttat aaaaccctca attgtgtcta
     4681 cgcttatggg tcttttttca caggccttga gcattccatg tataagcttg ctcttatcaa
     4741 agacctcttt gctgccgtct tttttaacta ccagaaagtt cacctcttcc actctctcat
     4801 aggtggtaaa cctcttgtta catgccaggc attcccttct gcgccttact gcaagcccgt
     4861 catttgtaag acgtgaatcc ataaccttat tttccagatc actgcaaaat ggacatttca
     4921 tttattaaag cagctcctta tatataggaa agcttgtgca gagttctttg acctgcccct
     4981 caatcttctt aagctcgcct tcgctgtctc tgttttcaac agcccttgct atcatgtctg
     5041 atatagtaat catctcgctt tctttcatgc ctcttgtggt aatagcagga gtacctattc
     5101 ttatcccgct tgtaatcatt gggggattag gatcaaaagg tacggcattt ttgtttaccg
     5161 taataccagc tttttcaagt gcttcctcag tttcttttcc tgtaagctct ttatttgtaa
     5221 gatcaacaag cacaaggtga gtatccgtgc cgcctgtaag aatattaaac ccatgctcct
     5281 gcagcctctc ggccatggtc tgagcatttt ttacaatctg tttttgataa tctttaaact
     5341 ccggctgcag ggcttcaaga aatgcaacgg cctttgcagc aattacatgc attagcggcc
     5401 ctccctgagt gccgggaaac accctgctat taataacctt agcatgttct tcatctgtca
     5461 tgaccatacc gccccttggg cctctaagtg tcttatgtgt agttgttgtt acaaaatggc
     5521 agtgaggtac ggggtttgga taaaggccgg tagctacaag acccgatgga tgggctatat
     5581 ctgccattaa gagcgccccc acttcgtctg ctatttctct aaatatttta aaatcaattt
     5641 tccttggata tgcgctccac cccactacta taagctttgg attatgcttt tgagcaaggc
     5701 ttcttacctc atcatagtca attaaggaat cttctttttt aaccccgtag gcaactatat
     5761 tgtatagcct gcctgaaaag tttacagggc ttccatgagt aagatgaccg ccgtgggcaa
     5821 ggttcattcc cagcaccgta tcaccaggct gaactgctgc aaaatataca gccatattgg
     5881 cctgtgctcc cgaatgaggc tgtacattta cagcatgcgc cccaaaaagc ttctttgccc
     5941 tttctatagc aagggtttct gctatatcca caaactcgca tcctccatag tatcttttgc
     6001 ccggaagacc ctctgcgtat ttgttggtta gtaccgtccc taaagcctcc ataacggctc
     6061 tgcttacata gttttctgaa gctatgagtt caagcttcca ttcctgtctt gcaatttcag
     6121 atttaataac atcatatatt tcaggatcag tgtccattat agattgcatt aataataaaa
     6181 cctccaaaaa aattactgga atgttatcta actactggtg tataagcaag aaatatgtca
     6241 taaattgtat ataattacta gagaacatac tacaaatggt atttaaattc atttcatctg
     6301 ttattgatta attacaattg attcatattc tatatggtat agaatcagct ttttaacaag
     6361 ttacggcagg aatataaatt tgataaagaa tacaaaaaaa gcgatatttt ttgattttgg
     6421 agacacactc gcctctaccg atccgccata tattttcagg atagcaagtg ggtttagaag
     6481 agccggctat gatatttcag atattgactt tgagcacgcc tatcttaggg cagactttga
     6541 gatatttaaa acacatcaat cccagggcag tatgaattcg tctcaataca atagtttgtt
     6601 tttccctctg ctctgcaagg cgctggatat taaagaagac ccggatgatg tcagagcaaa
     6661 tatcagaaaa aatactactc caagaaggtt ttacagaaat gcccttccag gcacacaaaa
     6721 acttcttaat tacctaaagc aaaaaggata tatacttgga atcatttcca ataatgacgg
     6781 ctatactaac aaaaagtgca aggacgtgga tattcacaga ttttttgata tcatcgcaga
     6841 ttctacaaac attggatatg taaagcccga tcccagaatt tttcatcatg ttttagatga
     6901 attgggctta gaccctgagg actgccttca tgtgggtgac ctctatggag cagacgtatt
     6961 gggtgcaaaa aatgctggca ttgaggtagt atggctgaat taccgcggca ttaatattat
     7021 gaatgacaca gagaccgttc agattaaaga gcttactgag cttattgaat tgctctgata
     7081 acagcacgat ataatataag taatattaca aagaataacg aaaaaattgg aaaagtttat
     7141 tcatgtaaaa ggggcaaggc taaataacct aaaaaacata gatgtaaata tcccccttca
     7201 caaattcact gttgtcacgg gtcttagcgg ttcaggcaaa tcctcccttg ctctggatac
     7261 aatatatacc gagggactta gaagatacat agagagcctt tccacctatg caaggcagtt
     7321 tctagaccgg gttgaaaaac ctgatatcga tgaaataaaa ggcatcccgc cggcaattgc
     7381 agtagagtca agaaattcta taaaaaactc ccgttccact gtgggaacaa taactgagat
     7441 atacgactac ttacgcctgc tttttgcaaa agtgggccgt ctcatctgtc cgaactgccg
     7501 tattgaagtc aaaaaacact ctcctcaaag cattgctgaa tttgtattaa aagatttttg
     7561 tgagcaggtg ttaatcatta caattaaaac tacaatagat gaccgaatga accccgatat
     7621 tccactttca aaaggattta caaaaatact cagggacggg cacattcatg atattgaaga
     7681 gcttgatgga cttaccgata aagatgaaat agtggttgat agattaaggg taaatgaaga
     7741 taataaatca aggcttatag actctaccga atctgggttt attcaatcta atgaacttat
     7801 atttaacatt ggcaaagaca gtatattcag atttacaaaa aagcttcagt gcccaaaatg
     7861 cacaaaagag ttttcagaac caactcctca aatgttttca tttaacagcc cctccggagc
     7921 atgcagagag tgcagcggat ttggcaatac gctcgatctc gatcccgatc ttgtagtacc
     7981 agacccggac aaaagcttat atgaaggtgc aatcgaaccc tttacaaaac cctcgctcga
     8041 tcatgcaaga acaaggctta tgcactttgc aaaaaaatcg ggaataaata cgaatgttcc
     8101 atataaagac ttatcggatg agcaaaagga acttatcttt agcggtggag aaggttacag
     8161 gggagttagg ggatacttta agagactgga aggtaaaaac tataagctgc atgtaagagt
     8221 tttcctgagt aaatacagat cggcttatac ttgcagcgtg tgccatggca caaggatagt
     8281 gcccgaggct ctttggttta agatcggtgg gaaaaatata tccgagattt ccggtatgcc
     8341 tgcagatgag ctggccactt ttattaataa gctcaaactc tcagattatg aatcggaaat
     8401 tgcatctgag atactaaaac agattaaatc cagagtggga tttctgctaa aagtcgggct
     8461 tgattattta actctatctc ggctggcaaa gactctttcc ggtggtgaag cacagagggt
     8521 gaatctttcc tgtcagctag gtggagggct tacagaaact ctttatatac tagatgaacc
     8581 ctcaatcggt cttcatcaaa gagatatcga ccagctgatt gcaatcataa aggaactcac
     8641 acaaagaaat aacaccgtga tccttgtaga acatgatatg gatatgataa gagcctcaaa
     8701 ctatattgtc gagcttggcc ccaaatcagg cgaacatgga gggcagcttg tatttgaagg
     8761 aagcaaatcc aaatttacaa agaattcatt tgagtccata accaaatcat actatcttgg
     8821 tgaaaaagag attgaggttc caaaaaaaag aagaaaggga aatggtaaat atataactat
     8881 ttatggtgca aaggaaaaca atttaaaaag tatcgatgtg tcatttcctc tgggcacatt
     8941 tatctgtgtt accggcgttt ccggctcagg aaagagttct ttacttaatg acatcttata
     9001 ctcagccctt gcaagaaaat ttaattcaga cattgagagg gttgggaatt ataaatctat
     9061 taaaggaaca gagcatatat caaatattta cctgctagac caaaaaccca ttgggaagag
     9121 ctcacgttcc aaccctgcaa catatctaaa aatttatgat gatataagaa aaatttttgc
     9181 cgacacctgg actgcccggt ctaaggggct taaaccatcc cacttttcct ttaatgtaca
     9241 aggtggcaga tgtgataaat gtgagggcga ggggcaggaa accgtggaga tgcttttttt
     9301 ggctgacgta catataacat gtgaggagtg taaaggtaaa aggtttaaag atgaggtact
     9361 aagcatacaa tacaaaaaga aaaatattga tgaggtatta cagctaacta tagatcaagc
     9421 agtatcgttt tttcgagaaa cgccaaagat agttcaaaaa ctaaatgttt tgtctgatgt
     9481 aggacttgga tacttgcggc ttggtcagtc ctctgcaaca ctttcaggag gtgaggcgca
     9541 gaggataaaa atagcaaagg agctttcagg taagaaaggt aaggatattc tctatatatt
     9601 agacgaaccc actgtcggtc ttcatattga tgatataaac cgacttctta aagttctcaa
     9661 taaacttgtt gactccggga atactgtaat tcttatagag cacaacttag acgtaataaa
     9721 atcagctgac catataatag atcttgggcc agagggcggg caaagcggtg gaaatattgt
     9781 tgcttgtgga actccggagc aaatcacagc catcgataaa tcttataccg gcaagtattt
     9841 aaaacctctg ttgtcgaatt aactttagat aatattattt tttcgaataa tattttgatc
     9901 atatctaaaa aatccccttg tattttttat gaccttgccc catataatag taccagatta
     9961 taagttagaa ttttaggtaa taaaccctat gaataggagg caagggaaat gcccagaaag
    10021 cgttacac
//
LOCUS       DBNGSV010000044         7270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_560667, whole genome shotgun sequence.
ACCESSION   DBNGSV010000044 DBNGSV010000000
VERSION     DBNGSV010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_560667"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..885
                     /gene="trpC"
                     /locus_tag="AC159U_01595"
     CDS             103..885
                     /gene="trpC"
                     /locus_tag="AC159U_01595"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR3335782.1"
                     /translation="MILDDIIENKILEVEQSKKDAPLDKLLADLETCPEPKDFYSALN
                     SIEGEIRIIAEIKQASPSKGILTSDFDPVRIAGTYLEGGANAISVLTDSKFFKGSLQD
                     LQNVRRAVDIPLLRKDFIIDPYQIYEAKFYGADAILLIVAALSPDDLQRLKTTAESLG
                     LATLVEVHNEQELQTALGLNCRIIGINNRDLKTFNVDMQTSINLKKLIPDDILVISES
                     GIKSPKDIQKLKSSGITTFLIGESFMKSDDPVSKLNELKNLD"
     gene            935..1252
                     /locus_tag="AC159U_01600"
     CDS             935..1252
                     /locus_tag="AC159U_01600"
                     /inference="COORDINATES: protein motif:HMM:NF040144.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HIR3335783.1"
                     /translation="MKIRRTHIAIILSALFPGFGQIYNRQYNKGIFYICFKLVINLLR
                     KEPMEMMLAAKKAGDIAVDNNTVLIVAAYTIADIILWILSVVDAKRNAEIIEKQIENK
                     SDE"
     gene            1308..2132
                     /locus_tag="AC159U_01605"
     CDS             1308..2132
                     /locus_tag="AC159U_01605"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3335784.1"
                     /translation="MRRIIFILLLLVLQTLQSTAQNTIVADRVFRANRHNLHFPNIST
                     DYPFVDLRSSHIIQKAYVDLKQKDDKIAGYKAALTSKKAQARFSAATPVAGVLFESGK
                     LHSPDIIKLDDARNLLIETEIGYLVDNDIIQTIESYNELGGLITKVYPVVELPDVGYK
                     DLSKINVIDIISTNAGSKNYIFGKRHSIEQIDIDNIKTKLFHDGKMISSGISTNVMEN
                     QRSALLWLINDILSRGYEIKSGHILLTGAFGTINKAQTGLYMGDFGSLGKIYFEIK"
     gene            2153..2617
                     /locus_tag="AC159U_01610"
     CDS             2153..2617
                     /locus_tag="AC159U_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024940.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3335785.1"
                     /translation="MKQIFVRDLKKSEINHLYKGLNSASAFVLRRCQIILSSAEGKNS
                     TQISQILYCSDQCVRDAINTFNKVGLKCLTEKSHARHDNQKKFSESAIKQLITMVKDS
                     PRNYGLKTDVWTLDKLAKTCTKNKLVSKSVSPGTVATILKQKKINWKEIKEP"
     gene            complement(2720..3880)
                     /locus_tag="AC159U_01615"
     CDS             complement(2720..3880)
                     /locus_tag="AC159U_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019268.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3335786.1"
                     /translation="MSKMTKLLIALVFAAATITAGQNTRAEEDADVSGLIKMFESISV
                     GFSLDTTYQYSFNEPDSGDIGARSLYPEDNEFNLNAFTLTFEKTPTADGTVMDHLGFR
                     ADILFGEHAELINPSGIDSDVVSPYQAYIQVMPVEGLNFYFGQFVTLAGFEVIEAKDN
                     PNISRAILFGFAIPFTHTGIRATYETGPFSVALGVNNGWDQWDDDNDAKTIEAQLGLS
                     GGNDSMEGSLYVTGYFGKEDDGLGNDEWRELVTVVGSLAFGPLSLYADLDFGWQEIAG
                     PDDDAFWWGIAGYAVLDAHPMIDLALRAEYFDDDDGFRGFGTTVWEVTPTVIFKPFKG
                     MPNSDHSAYLDNFEFRLEYRFDKADAAIFEDEGGADDTQHTVMAQALYWLNL"
     gene            complement(3940..5196)
                     /locus_tag="AC159U_01620"
     CDS             complement(3940..5196)
                     /locus_tag="AC159U_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3335787.1"
                     /translation="MSSFAQEVEEGPTASHAVFVANNVWMLVATFLVFIMHLGFAALE
                     TGLTRSKNTVNILFKNVSIISIGILTYAICGFNLMYPGEFGGWLGFAGFGISSPEGAA
                     GLIEYADGGYTYWTDFIFQAMFAATAATIVSGAVAERIKIESFLVFSIIFVAIVYPIA
                     GSWKWGGGWLDAKGFYDFAGSTLVHSVGGWGALVGAFLLGPRLGKYVRGGMKPIPGHS
                     LPLAAIGVFLLWFGWYGFNGGSVLSADPELVSLVFVTTSLASAAGVVGAMVVSWIMFN
                     KPDLTMALNGALAGLVGITAGADVISPMNSVIVGLIAGVIVVFSVVSFDKIKIDDPVG
                     AISVHLVCGIWGTLAVGIFSADHSVLIQLLGIAAYGVFTVICAFALFLLIKVTMGLRV
                     DEQEEMEGLDFSEHGLESYPDFSKSN"
     gene            complement(5546..5842)
                     /locus_tag="AC159U_01625"
     CDS             complement(5546..5842)
                     /locus_tag="AC159U_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3335788.1"
                     /translation="MAFIIIGPFLGFFGTGFYSGFGALFAEVFPTRARGTAQGFCYNV
                     GRLVGSLAPPLAGYVYAAFDNNYGPAFLMLSLFAVAAAITIMTFPETKGKDLEI"
     gene            complement(6017..6187)
                     /locus_tag="AC159U_01630"
     CDS             complement(6017..6187)
                     /locus_tag="AC159U_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335789.1"
                     /translation="MDISIPAHKVLGSPNTYGSVIPYGITNGVKANIARIITIIERQL
                     QILISIFPYFIE"
     gene            6258..>7270
                     /locus_tag="AC159U_01635"
     CDS             6258..>7270
                     /locus_tag="AC159U_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3335790.1"
                     /translation="MDKGITFIVVAIIYLIVLISLAVIGNRRNKGDADQFFLAGGNLG
                     IIIGFLTFFATLYSTFILIGMPNFFRVHGVGTWIFLGVTDMVLAFFTLWLAMKMGKRV
                     SSVGYRSMSDIIKDDFGTPGTIVYVIGLVIFLVPYIAIQVKGASGLLSSVVPFPVPQW
                     VWAITILVLMYIYSSIGGLRGIMYADSLQGVMLFTVIWIMAAIILMDFGGIKELISSV
                     KETKPELLSTPGPNGLLNVQWLLAAFISIVLLPVSQPQLTSRLGILKGFNERRKVPVL
                     HASFTFLLLIAGLIIGLYGAVAIVVMERSIKSIRIPNIMFGTTPKSDIRNITRLAAVK
                     VGIT"
BASE COUNT         2404 a         1417 c         1332 g         2117 t
ORIGIN      
        1 gggagtctat cgacagcgga aatgctatta aggcattaga aaaagtaata aagtttacta
       61 ctacatggaa ctaatattaa gtttaataag atttaattta aaatgattct tgatgatata
      121 atagaaaata aaatactcga agtagagcag tccaaaaaag atgctcctct tgataaactt
      181 cttgcagacc tagaaacctg ccctgaacct aaagattttt acagtgcatt aaactccata
      241 gagggagaaa tacgtattat agcagagatc aaacaggcat ctccatccaa agggatatta
      301 accagtgact ttgaccctgt aagaatagcg ggcacatacc ttgagggtgg agccaatgca
      361 atttctgttc ttacggactc aaaatttttt aagggaagtt tacaagatct acaaaatgta
      421 agaagagctg tagatatacc acttttaaga aaagacttta ttattgatcc ctatcagata
      481 tatgaagcaa aattttacgg ggctgatgct atactgctca tagttgcagc actaagccct
      541 gatgatctgc aaagattaaa aacaaccgct gaatccttgg gtcttgctac ccttgtagaa
      601 gttcataacg aacaggaact gcaaacagcg ttaggtctaa attgcaggat aataggcatt
      661 aacaacagag accttaaaac ctttaatgta gatatgcaaa cttcgattaa tcttaaaaaa
      721 cttatccctg acgatatttt agttataagt gaaagtggaa ttaaaagtcc caaggacatt
      781 caaaaattaa aatcaagtgg aattacgacc tttcttattg gagaatcatt tatgaagtcc
      841 gacgatcctg taagtaagct taatgaactc aaaaatctcg attaatctgc tacattagca
      901 tatctctaac tgattgatgt cttaaaccaa atatatgaag ataagaagaa cacatattgc
      961 cattattcta tctgctctgt ttccggggtt tggacaaata tataacaggc aatataacaa
     1021 gggcatattt tatatttgtt ttaagcttgt tataaattta ctaagaaaag agcctatgga
     1081 aatgatgctt gcagccaaga aggcagggga tatagctgtt gacaataata cagtgcttat
     1141 tgtagcagcc tacacaatag ctgatattat tttatggata ctctcggtag ttgatgccaa
     1201 aaggaatgca gagattattg aaaaacagat agaaaacaag agtgatgaat aaattatttt
     1261 ataatattta tttggcattt ccataacaat aaaatagtat tttcgttatg cggcgtatta
     1321 tctttattct cttactgtta gtactgcaaa ctcttcaatc taccgctcag aatacaattg
     1381 ttgctgatag agtttttagg gcaaacagac acaacctgca ctttccaaat atttcaacag
     1441 actatccatt tgttgacctt cgctcatctc atataatcca aaaagcatat gtagatctaa
     1501 aacaaaaaga tgataaaata gcaggttata aagctgctct cacttctaaa aaagctcagg
     1561 ccaggttttc agctgccaca cccgtggcag gcgtgctgtt tgaatcagga aagttgcatt
     1621 ctcctgatat aataaaattg gacgatgcaa gaaacctgct cattgaaaca gagattgggt
     1681 accttgtaga taatgacata atacaaacta ttgaatctta caatgagctt ggcggcttaa
     1741 taacaaaagt ttatccggta gtagagttgc cagatgtagg gtataaagat ttatctaaaa
     1801 ttaatgtaat tgatattatt tcaaccaatg caggttctaa aaattatatt tttggcaaac
     1861 ggcatagtat agaacaaata gatatagata atatcaaaac aaagcttttc catgacggaa
     1921 aaatgataag ctcgggtata agtacaaatg ttatggaaaa ccaaagatct gcgcttttat
     1981 ggttaataaa tgatatcttg tccaggggat atgaaattaa atccggccac attttattaa
     2041 caggagcttt tggaactatt aataaagctc agacaggcct ttacatgggt gatttcggat
     2101 cactcggaaa gatatatttt gaaataaaat aaaagggtat tttgtttgtt ttatgaaaca
     2161 aatttttgta agagatttaa aaaagtctga aataaatcat ttgtataagg gtcttaactc
     2221 ggcaagtgca tttgttctca gaagatgcca gatcatacta agcagtgcag aaggaaaaaa
     2281 ctcaactcaa atttctcaga ttctatactg tagtgatcag tgtgtaaggg acgcaattaa
     2341 tacatttaac aaagtgggac ttaaatgtct taccgaaaaa tcacatgcaa gacacgacaa
     2401 tcaaaaaaag tttagtgagt ctgcaattaa acagctaatc actatggtaa aagattctcc
     2461 acgaaactat ggattaaaaa ctgatgtatg gaccctggat aaacttgcta aaacatgcac
     2521 taaaaataaa cttgtctcaa agtctgtatc acccggcaca gtggctacaa tattgaaaca
     2581 aaaaaaaatt aactggaaag aaataaaaga accgtgagca gcccacaagg aggagagctg
     2641 ctcacagata ttgtaaactg tcccagcgtc aaggaggtat ggagagcata aagcctagtc
     2701 cgttgggaac ggtgttacat tacagattga gccagtataa agcctgagcc attacagtgt
     2761 gctgtgtatc atcagcccca ccttcatcct caaatattgc tgcatctgcc ttatcaaatc
     2821 tatactctaa tctaaattca aaattatcca gatatgcgct gtgatcacta ttaggcatac
     2881 ccttgaatgg tttgaaaatt acagttggag ttacttccca tactgttgtt ccaaaacccc
     2941 tgaagccgtc gtcatcatca aaatattcag cacgaagcgc aagatctatc attggatgag
     3001 catccagcac tgcatatcct gcaatacccc accagaatgc atcgtcatca gggcctgcaa
     3061 tctcctgcca tccaaagtca aggtcagcat agagtgaaag aggaccaaac gccagtgaac
     3121 ccactaccgt tacaagctct ctccattcgt catttccaag tccgtcatct tctttaccaa
     3181 agtaacccgt aacatataat gatccttcca tggagtcgtt gcctcctgaa agaccaagct
     3241 gtgcttcaat ggtttttgca tcattatcgt catcccactg atcccatccg ttgttcacac
     3301 caagtgcaac agaaaatggt ccggtttcat aggttgccct tatacccgta tgggtaaatg
     3361 gtatagcaaa gccaaacaga atagccctgc ttatattagg attatccttt gcttcaatca
     3421 cttcaaaacc cgcaagtgtt acaaactgac cgaaataaaa atttaatcct tccactggca
     3481 taacctgaat atatgcctga tatggtgaga ctacatcgga gtctataccg gatggattaa
     3541 taagttcagc atgctcacca aacagtatat ctgccctaaa acccagatga tccatcacgg
     3601 ttccgtctgc agtaggtgtt ttttcaaatg taagagtaaa ggcgtttagg ttaaactcat
     3661 tatcttctgg atataatgat cttgctccga tatcaccact atctggttca ttgaagctgt
     3721 actgatatgt agtatccaga gaaaaaccta cgcttattga ctcaaacatc tttataagac
     3781 ccgaaacatc ggcgtcttct tctgctcttg tgttctgccc tgctgtaatt gtagcagccg
     3841 caaatacaag cgctattaat aactttgtca tcttactcat actctaattc cctcctttaa
     3901 aataatgatt tgtatatttt gtcatttctc atttaatttt taatttgatt tagagaaatc
     3961 cggatatgac tcaagaccat gttcagagaa atccagacct tccatttctt cctgctcgtc
     4021 tactctaaga cccatggtta ctttaattaa tagaaataga gcgaaagcac atataaccgt
     4081 aaatactccg tatgctgcta tacccagcaa ctgaattaac acgctgtgat cggcgctgaa
     4141 tattccgact gcaagtgttc cccatatacc gcatacaagg tgaacagata tggcacccac
     4201 cggatcgtca atcttgatct tatcaaaaga tactactgaa aatactacta ttacacctgc
     4261 tattaatcct actatcactg agttcatagg gcttattaca tctgcgcctg ctgttatccc
     4321 aacaagacct gcaagagcgc cgtttagagc catagtgaga tcaggtttat tgaacataat
     4381 ccatgaaaca accatagcac ctacaactcc tgctgccgat gcaagggatg tagtaacaaa
     4441 taccagtgaa acaagctccg ggtcagctga gagaactgaa ccgccgttaa aaccgtacca
     4501 gccaaaccat agcagaaata cccctattgc tgcaagcggt aaactgtgac cggggattgg
     4561 cttcattccg cctcttacat atttaccaag cctcggccca agaaggaatg cacctaccag
     4621 tgctccccag ccgcctacgg aatgcaccag ggtcgaaccg gcaaaatcat aaaaaccttt
     4681 tgcatccaac catccgccgc cccatttcca tgaacctgcg attggataaa caattgctac
     4741 aaaaattatt gaaaacacaa gaaaactttc aatcttgatt ctctcggcta ctgcaccgga
     4801 aacaatagtt gcagcggttg cagcaaacat cgcctggaat ataaaatcag tccagtaagt
     4861 atagccgcca tctgcatatt caatcagtcc tgcagcgcct tcaggagatg aaattccaaa
     4921 acctgcaaaa cctaaccagc cgccgaattc tccggggtac atgagattaa atccgcagat
     4981 tgcataagta agtatgccga tggaaatgat tgatacgttt ttaaaaagta tgtttacagt
     5041 gttcttggaa cgagtaagac ctgtttcaag agccgcaaat cccaggtgca taataaatac
     5101 aaggaaagta gcaacaagca tccatacgtt atttgctaca aatactgcat gtgaagctgt
     5161 aggcccctct tctacctctt gtgcaaaaga gctcattgta ccaaaaactg ctattaccag
     5221 taacgacatg atacaaaagt agattgattt tcttttctcc ggtgcaaatc tcaacatctc
     5281 taatacctcc atcttgaagt cggttttcgg ctttaaattg caatgtgtat gccaagtttg
     5341 acagggcggc gtaacatact gtaactattg aattatttca tattacctaa ataaaatagg
     5401 gtatatattt atagaattgt gcttattatt taagcacgtg tgatgggata aaaagcacag
     5461 aatagagtta taatattaag gcagagaaat ccttaattta aaataagaaa ttctatctat
     5521 tatgcatata tcagtagatt agatactaaa tctccagatc cttacccttg gtctccggaa
     5581 atgtcattat agtaatagcc gcagccacag caaatagcga aagcataagg aatgcagggc
     5641 cgtagttatt atcaaacgct gcatatacat aacctgccag aggaggcgcc aaagacccta
     5701 caagtcttcc tacattgtaa caaaaaccct gtgccgtacc tcttgccctg gtaggaaaga
     5761 cctcagcaaa aagagcgccg aagcctgaat agaaacccgt tccaaaaaat cccaggaaag
     5821 gaccaatgat aatgaatgcc aacagcctgc taattcctag ataatatgct gatatcatta
     5881 tattaattaa attaacagcg ggcatgattg tcgtatatta cgaattttgt acttaatttc
     5941 taggcatata tgtcatctaa aatagcacag gctagattga cacattcaaa ttaatttagt
     6001 acgattgctt tcaatattat tcaataaaat aggggaatat cgaaatgagt atttggagct
     6061 gtctttcgat aattgtaatt attctagcaa tattggcttt tactccattt gtaattccat
     6121 atggaattac agagccatat gtgttaggac taccaagaac tttatgggca ggaatgctta
     6181 tatccatatt tatatattta attcttttat tggctatgat tacatcaaaa gatgaggact
     6241 aattttgtaa ggtttgtatg gacaaaggta taacatttat tgtagttgct attatttatc
     6301 ttatcgtact cataagcctt gccgtaattg gaaacagaag aaacaaaggt gatgcagacc
     6361 aattttttct agccggtggg aatttaggaa tcattatagg atttctgact ttttttgcca
     6421 ccctctacag tacatttatt ttaataggaa tgcctaattt ttttagagtt catggtgtgg
     6481 gaacatggat atttttaggt gtaactgata tggtacttgc tttttttacg ctttggctgg
     6541 ctatgaaaat ggggaaaaga gtttcatcag taggttaccg aagtatgtca gatataataa
     6601 aagacgattt tggtaccccc ggaaccatag tatacgtaat tggccttgtg atttttcttg
     6661 tgccatacat tgcaattcag gtgaaaggtg catcggggct tctatcgagt gttgtaccat
     6721 tcccggttcc tcagtgggtt tgggcaataa caatactagt attaatgtat atttactctt
     6781 caataggagg actcagggga attatgtatg ctgattcgct tcagggagtc atgcttttca
     6841 cggttatatg gataatggcc gctataattt taatggattt tggaggaata aaagaactta
     6901 tatcttctgt aaaggaaaca aaaccagagc ttctttctac tcccggaccg aatgggctat
     6961 taaatgtcca atggcttctg gcagcattta tatcaatagt tctattgccg gtttctcaac
     7021 ctcagcttac atccaggctt ggtatattaa aaggatttaa tgaaagacgc aaagtgcctg
     7081 ttttgcatgc atcctttaca ttcctgctcc tgattgccgg cttaattatt ggtctttatg
     7141 gcgcagtggc aatagtggta atggaaagga gtatcaaatc catccggatt cccaatatca
     7201 tgtttggaac tactccaaaa agtgacataa gaaatattac aagactagct gctgtaaaag
     7261 taggtattac
//
LOCUS       DBNGSV010000045        16631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_630828, whole genome shotgun sequence.
ACCESSION   DBNGSV010000045 DBNGSV010000000
VERSION     DBNGSV010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16631
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_630828"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="AC159U_01640"
     CDS             <1..589
                     /locus_tag="AC159U_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3335791.1"
                     /translation="YEYMERASKHLSMVIPLTLLIVIVLLYMNTRSMVKTGIVLLALP
                     FSMVGAVWFLYIAGFNLSVAVWVGIIALLGVDAETGVVMLLYLDIAYEKFKKNGLLKS
                     MADLRSAIFEGAVKRVRPKMMTVMTTMLGLLPILIGLGAGSDVMKRIAAPMVGGILTS
                     FIMELTVYPAIFYTIKRREVKKLMNSNEQEIISNN"
     gene            976..1902
                     /locus_tag="AC159U_01645"
     CDS             976..1902
                     /locus_tag="AC159U_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3335792.1"
                     /translation="MQTIISIENLEKKYASGLHALKGINLEIYNKEIFALLGPNGAGK
                     TTLISIICGIVNPSGGKVLVDGHDIISDFRAARAKIGLVPQELSTDVFETVWASVNFS
                     RGLFGKPPNRDHIEKVLKTLSLWDKKDSKIMALSGGMKRRVLIAKALSHEPQILFLDE
                     PTAGVDVELRRDMWDMVRRLRESGVTIILTTHYIEEAQEMADRVGVINNGEIIIVEDK
                     NVLMEKLGKKRLTLYLQNPLSKIPDGLNGYSLELSADGYELVYSFDVQSEETGIADLL
                     RLLNEHGIDFKDLNSSESSLEEIFVSLVNKKQ"
     gene            1899..2660
                     /locus_tag="AC159U_01650"
     CDS             1899..2660
                     /locus_tag="AC159U_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3335793.1"
                     /translation="MNIYAIKAIYKFEMARAWRTLMQSIFSPILSTSLYFVVFGAAIG
                     SRMGDIEGVTYGAFIIPGLIMLSLLSESISNASFGIYMPKFAGTIYEVLSAPISAVEI
                     VIGYVGAAATKSIILGLLILATARLFVPYEIEHPFYMFCFLVLTAVTFSLFGFIIGIW
                     ADGFEKLQIVPMLVVTPLTFLGGSFYSINMLPPIWQKITLFNPVVYLVSGFRWSFYGI
                     ADVNIFVSLGMTFVFLILCLIGISWIFRTGYKMKA"
     gene            complement(2692..3348)
                     /locus_tag="AC159U_01655"
     CDS             complement(2692..3348)
                     /locus_tag="AC159U_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3335794.1"
                     /translation="MDTKTRINQIYSALNERYGPQNWWPAETELECIIGAILTQNTTW
                     KNVEKAIGNLKQHELISIEKLTCIQAEQLAQLIRSSGYYNQKAIKIKSFISFINDNYS
                     GNLGDMFSEQLYDLRQKLLSIKGIGPETADSIILYAAKKPIFVIDAYTHRILSRHGLV
                     PEYTSYTEMQELFMNSLPDDHQLFNEYHALLVKAAKEHCKKKSPICRGCPLEFDPHEV
                     "
     gene            3417..4505
                     /gene="gcvT"
                     /locus_tag="AC159U_01660"
     CDS             3417..4505
                     /gene="gcvT"
                     /locus_tag="AC159U_01660"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3335795.1"
                     /translation="MKKTALYDNHKKSGGRIVEFAGWQMPVQYEGLKQEHLAVRSSVG
                     IFDVSHMGEVEIKGSDAKEFCQRLTSNNINKIVDNQAQYSVMCNHNGGAVDDVISYKF
                     SDEHFFICVNAANTEKDYNWILQIKDEFKFESEVINASIEYSQIAIQGPDAASLLTKL
                     MGQEISSIKRFRFDKINWNGTESIIARTGYTGEDGYEVFLPWKKGPALWDELLNLGNE
                     FGIKECGLGCRDTLRLEMGYPLYGNELNDTTNPIESGLGFFVKHKDEDNDFVGRKSIL
                     DKIESGLKLKLVGFEMTDRGIARHGYNIIHGSEVVGVVTSGTHSPSLDKSIGMGLINY
                     HCEENNLCVDIRNKYRKIEIVDIPFYKK"
     gene            4525..4917
                     /gene="gcvH"
                     /locus_tag="AC159U_01665"
     CDS             4525..4917
                     /gene="gcvH"
                     /locus_tag="AC159U_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3335796.1"
                     /translation="MLIPEDLKYTSEHEWVREESELVVVGITDYAQEALGELVFIELP
                     SEGDEINKGDTFGAVESTKSVSDLFAPISGEVIEINEVLLDSPELINQDPYGEGWLIK
                     VKPFEDADLSDCLDFEDYTDIVEQELEK"
     gene            5026..7917
                     /gene="gcvP"
                     /locus_tag="AC159U_01670"
     CDS             5026..7917
                     /gene="gcvP"
                     /locus_tag="AC159U_01670"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR3335797.1"
                     /translation="MDYPLDSFVHRHIGPDSKDIKEMLSTVGCSSLDELIDQTVPQNI
                     RSNKPLKISAAETEFDYLSNLRSISQKNKVYKSYIGLGYYDTITPAVILRNIFENPGW
                     YTPYTPYQAELAQGRLEALINFQTMVIDFTGMEIANASLLDEATAAAEAMTMAARLRD
                     RKSEEQGQNKFLVSDKCFPQTIDVLKTRAEPLDIELVISDINQAELSEQYFGIILQYP
                     DQFGQVEDYSQIIQNAKDLDIKIIFAADLLSLTLLKAPGEMGADIVVGSTQRFGVPMG
                     FGGPHAGYFATKEENKRHIPGRIIGVSVDSNGRSAYRMSLQTREQHIRRDKATSNICT
                     AQALPAVLAGMYAVYHGPDGLRAIANKIHSLTRLLDNNLKELGFVQNNEHYFDTLTVD
                     LSNHSASILDELKKQCIKNKINLRYIDENTIGISVDETKSVKDIKKLINIFSSSIGKQ
                     SDSASSDDDLNRVNPKIPENLQRNSAYLTHPIFNTNRSETQMMRYIKKLENRDLSLNT
                     SMTPLGSCTMKLNAASEMIPVTWEEFSAIHPFAPADQTKGYAQIIKELQKDLIEITGF
                     KGVSLQPNSGAQGEYAGLMVIRAYHKDRGEENRNIVLVPSSAHGTNPASAVMCGMNVV
                     VVKCDNDGNIDINDLKKKASAHKSELAAIMITYPSTHGVFEETIKQVCKVVHRNGGLV
                     YMDGANMNAQVGLTYPGTFGVDVCHLNLHKTFSIPHGGGGPGMGPICVGRKLLKYLPG
                     HVYASVGGPKAISAVSATPWGSGSILLISYGYIKMLGAAGATEATKIAVLNANYIKSR
                     LGNNYKILYQGKNGRVAHELIIDLREFKRSTDIEVEDIAKRLMDYGFHAPTVSWPVAG
                     TMMIEPTESEPKEELDRFCDALISIYEEINDIARGKYEKDNNVLKNAPHTAAYVSSDE
                     WSNSYSRELAAYPEAYLRENKFWPPVGRINNPYGDKNLVCTCIAVEDYE"
     gene            complement(7944..8867)
                     /locus_tag="AC159U_01675"
     CDS             complement(7944..8867)
                     /locus_tag="AC159U_01675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3335798.1"
                     /translation="MQKIRTTIITGLIIMITGICYATEVIDRIVAVVNDDLITLSDLN
                     RYISSIPPGTAEIDRDRVLNDLVEQMILEQEAAKLGITVSDPEIDMSIENIRTKAGMS
                     HEQMEEMLKKENLTKEQFRKQWRLQIISGKLVNSIVKGRVAVTDDEIENLYKEYYGNI
                     ENSDEVQIAHILISVNEQQPERALEKASRIAELAKSGNDFSKLVAEYSDDTFSKEKEG
                     VLGYFKKGELVSELEAVVAITEVGEVSGPVQTASGFHIIKVLNRNTLDSNSVDTYREQ
                     LRQEIYKKKVAEELQKYIATMRENAYIEIKL"
     gene            complement(8854..9726)
                     /locus_tag="AC159U_01680"
     CDS             complement(8854..9726)
                     /locus_tag="AC159U_01680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3335799.1"
                     /translation="MIKTLILLSALIIVSCHDSSDKQSVKTKKPVVDNIVAKIGSDHI
                     TEKQLQDAISALPVKQKILIQSSPKQKNKFIESYINKEILYKEALKKGYGEKEDIRKS
                     VEQYKKKLMIRKLSEDILNQEITDKELEQYYESNKNLYQSATITQVSYIPKDIENLNQ
                     DELLNLQNEIISNLKEDADLGGLTNHINYKIKVKKNIEITRDKYSKLLGDVAFNMKKG
                     EISEPVKFGNSYVILKLIDGPNHIPFKNLKNNIKFEVKNNRFKSYLKNLKDLENIEIY
                     SYKETRETNSNAEN"
     gene            complement(9733..13164)
                     /gene="mfd"
                     /locus_tag="AC159U_01685"
     CDS             complement(9733..13164)
                     /gene="mfd"
                     /locus_tag="AC159U_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3335800.1"
                     /translation="MSTNKQSIIEHLSTRVNNKYIARHIVDTLDSNDKVYLGGLQGFS
                     KNFFASWLSLISQKQLLIITTDDKQAEQTQKELSYLTNSKVLYLPKKELDADRPLFTS
                     SSDVFSLRSNWLYDAQNHKILVCDIQTLLEKYPPRDFYDTCSIKININDKLDRTLLQQ
                     RLSAFGYTKTDFVQKKGDYSHRGLIIDIFSPCYELPLRIEFFGDSIHSVRFFDTVNQK
                     TVSKTDKASVIPTTELIVDQDNINYIKSSIKEFSNENGVSASYRNSLIHQLQNEQRPE
                     YINWLIPFCFKSLKSIFDYMNEDTLLFFYSGSEEKYFEQCLEDISEKLLVLKKKYKLI
                     PDLDSLYLSLPELKTKLSQIKKIYIPELPVSGKNSINLNFKNIDFHKQPENSPSPYNE
                     LADRLKQLQKEDYKILFVLKTKGETEKLAEIIKSRKLKDIQFEIGEIESGFVSEEFGI
                     AVITENEIYHQKKVKPVSKNDDIPSAFITSFSELKPGDYIVHKDFGVGIYRGLLQLDI
                     KGKKADFLVCEYKDGDKIYVPVDKLNIVQRYVGEGKSPTIEKLGKETWAKTLKKVKKS
                     IELIAKDLLQLYAKRKAEKGFRFSKNDTLYSELEQSFEHEETAHQVSAINDVMLDMES
                     DLSMDRLICGDVGFGKTEVAIRAAFKAVMDGKQVAVIVPTTLLCFQHYLTFKSRLKGF
                     PINVEMISRFRTSAQTNKILESLYLGNTDIIIGTHKLLGSKVHFKDLGLIIVDEEHKF
                     GVKQKENIRKIKTSVDVLTLSATPIPRTLQLSLANIRDISLINTPPAGRQQIETYVYH
                     FNENTIREAITNELKRGGAVFFIHNRIEDIHTIAKRITNIVPDVKYEITHGRMRESVL
                     ENSINKFINGDIDLLITTTIVESGLDISRANTIIINDAHMFGLADLYQLRGRVGRADK
                     KAYAYFLVPGSEKLTQSSKKRLKAISELKELGSGFKLALSDLEIRGAGTLFGTEQSGH
                     IGDIGLEMYLEMLESAVRNLSNQKQEFSFEPEIKSSYPTFIPNEYIEDDSERLMVYKK
                     LSSCRSLKELKEIYSDISDRFGRAPETVVNLFSLIEIKIYMKKFLIKKLELKKNSVVM
                     EFLENSSLFKYFRPSGIYRISEKSELTLKSVKEIMSSFNSKNPPYMNQPTNKTAH"
     gene            complement(13174..14256)
                     /gene="leuB"
                     /locus_tag="AC159U_01690"
     CDS             complement(13174..14256)
                     /gene="leuB"
                     /locus_tag="AC159U_01690"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3335801.1"
                     /translation="MPKILLLPGDGIGTEVTNESIKILNFLNEQENLGLEFEEALFGG
                     ACLDEHKIPITDKTIEIAMESDAVLMGAVGGPKWDSLPHEIRPERGLLRIRKELDAFA
                     NLRPAVVFGPLKNASTLKNEIVDGVDIMVVRELTGGIYFGTPRGIEYLDSDEKKGTNT
                     LSYTTSEIKRIARVAFEIAGKRKKKVTSIDKANVLEVMQLWRDTVTEVKETEFADIEL
                     EHLYVDNAAMQLIRRPKSFDVMLAGNMFGDIISDEAAQLTGSLGMLPSASIGTHGAIY
                     EPVHGSAPDIAGQNLANPIASILTTAMMLKYSLNLDSISNKIQSSVDSVLESGYRTGD
                     IYEQGSKKVNCTEMGDLILKDLQNNY"
     gene            14389..16245
                     /locus_tag="AC159U_01695"
     CDS             14389..16245
                     /locus_tag="AC159U_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3335802.1"
                     /translation="MAKKKRAKNTKNKDVGTSDVKTQVESEQLDELTLKLPSWLRGEV
                     FLSILFFVLAFVLYSFSLKNEFVWDDVEVIKKSYYNFENTSLISKFIPAVNETKKLLY
                     YRPTIPISYIIDWNNWNLNPFGYHLSNSIFYAFTVVGMFFLSNRITRLFSIKQALTFS
                     MLATLLFVLHPMHVESVSWIAGRTDVLCTMFFLFALGFHILSYKKFYLLPVVCLFYLF
                     SLFSKEVAVAFPLLIICMDYLVKRPITKADLFRYVVYFSLTGLYLYLRSRSFINIPQV
                     YNPDIVSDSQSPGQLLYYFGIFKVFIVSYAYYLKQLLFPYTFNAFVTYLPTQVLYLTV
                     SIILIAILVIFFFYSYLRNKKFITFNIFWVFLTLGPSVLIAIFAFIATPVSERYLFLP
                     TVGYCMLVAYLLTGFLDKPQTRTVSLVITCFLIGSFMFFTIDRQSVWQSRLNFWQDAS
                     SKSDHHAIPHINYGMALLDAGRTDEAISEFNISLKPDIQNTPTGRAIAANNLGVAYIN
                     KNNYEQAARTLLIALESDPGFYKTYYHFGLLYYIRAGKSKLEDDFATSERFLLKATSI
                     YPRYGKAYLLLSKLNSNFGNKEKAVHYATKALQSGLVNELAAQATKVIQMNK"
     gene            16298..>16631
                     /locus_tag="AC159U_01700"
     CDS             16298..>16631
                     /locus_tag="AC159U_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335803.1"
                     /translation="METNNLLPSIIKNRLIASLFLFLVSFAVFIPSLNNDFVWDDIPY
                     LLNRIEHLTISKQINKVIPRIKTNSNNKSDYYRPMLHISLALDNDIWNGKSLGFHLSN
                     IVMHSITTV"
BASE COUNT         4963 a         2911 c         3170 g         5587 t
ORIGIN      
        1 atatgaatac atggaaagag catcaaaaca tttatcgatg gtaattcctc ttacactttt
       61 aattgttatc gttcttcttt atatgaatac cagatcgatg gttaaaacag gaatcgtttt
      121 acttgcctta ccgttttcca tggttggcgc agtttggttt ttatacattg ccggatttaa
      181 tctttctgta gcagtgtggg taggaataat cgcactgctt ggtgtcgatg ctgaaacagg
      241 agtagttatg ctgctctatc tcgatattgc ttatgagaaa tttaagaaga atggtttact
      301 taaaagtatg gcagatcttc gttcagcaat ttttgaagga gcagtaaaga gagttcgtcc
      361 gaaaatgatg actgtaatga cgacaatgct tggtcttctt ccgatattaa tagggctcgg
      421 tgctggttcc gatgttatga aaagaattgc tgccccgatg gttggcggaa ttctgacaag
      481 ctttattatg gagcttaccg tttacccggc gatattttat acaataaaaa gaagagaagt
      541 aaagaagttg atgaatagca atgaacagga aataatttca aataattaaa taacaaataa
      601 tactaaaaac gggattgtat catgtgaccc taaggtgatt atgatttttt taattagaaa
      661 taaaattccg gcagcaaaaa atatagcggg gaaaaacatg actgcctgta cgaggaaaag
      721 cattgcagga actgttagaa ataaagcaat aaatattgtc catttaattg tacggtagct
      781 cataaggtat tataaaacaa atatacaaga aaagctctta ttaaagctaa tattaattta
      841 tgcttaatct ttagcctgtt tcaaatataa gtagcaaact cggttgaaaa actattttta
      901 ggcagcaatt agaatcttat gaattgatag atatacttac accctttatt aaattttatc
      961 gtctaaagaa atgtcatgca gactattata tccattgaaa atctcgagaa aaaatatgct
     1021 tcagggctcc atgcgctcaa gggcataaat cttgagattt ataataagga aatttttgcc
     1081 ctgcttgggc caaacggtgc tggaaagacc acgcttataa gtattatatg cggcatagta
     1141 aatccaagcg gcggaaaggt gctggtggat ggtcatgaca taatttctga tttccgggct
     1201 gccagggcaa aaatcgggct tgttccccag gagcttagca cagatgtgtt tgaaaccgta
     1261 tgggcgtctg taaatttcag caggggactc tttggaaaac ctccaaatcg ggaccatatt
     1321 gaaaaagtgt taaaaacttt gtcgctctgg gataaaaagg attccaaaat tatggcgctt
     1381 tctggcggaa tgaaacggcg cgtgctaatt gcaaaagccc tttcgcatga gcctcagata
     1441 ttgtttcttg atgaacctac agcgggggtt gatgttgagc taaggagaga tatgtgggat
     1501 atggttcgca ggctcaggga aagcggggtg acaatcatac ttacaacgca ctatatagaa
     1561 gaagcgcagg agatggctga ccgcgttggt gtgataaaca atggagagat aataattgta
     1621 gaagataaga atgttcttat ggaaaagctt ggcaagaaac ggcttacgct ttatctgcaa
     1681 aacccgcttt ctaaaattcc tgatggactt aatggatatt cacttgagtt gtctgccgac
     1741 ggttatgagc ttgtatacag ttttgatgta cagagtgaag agacgggaat tgctgatctg
     1801 ctgagactgc taaatgagca tggtatagac tttaaggatc taaattcctc tgagagctct
     1861 cttgaagaaa tatttgtgag tttggttaat aagaagcaat gaacatatat gcaataaagg
     1921 caatatataa atttgaaatg gcaagagcat ggagaactct catgcagagt atcttttctc
     1981 ctattctttc tacttctctt tattttgtag tctttggagc agctattgga tcaaggatgg
     2041 gagatataga gggagtaacc tatggagcat ttataattcc cggcctaata atgctatcac
     2101 ttcttagcga gagtatttcg aacgcatctt ttgggatata tatgccgaag tttgcgggga
     2161 ctatttatga agtattatct gcgccaatat cggctgtaga gattgtgatt ggttatgtgg
     2221 gagcagccgc tacaaaatca ataattttag gactcctgat cttagcaacc gcacgccttt
     2281 ttgtacccta tgaaatagaa cacccattct atatgttttg ttttcttgtg cttacagctg
     2341 ttaccttcag cctttttggt tttataatcg gtatctgggc agacggcttt gaaaaactgc
     2401 agatcgtccc aatgcttgta gtgaccccgc taacttttct tggtggaagt ttttattcaa
     2461 taaacatgct ccctccaatc tggcagaaga ttactctgtt taaccccgtg gtttatctag
     2521 taagcggatt cagatggagc ttttacggca ttgctgatgt aaatatattt gtaagcctgg
     2581 ggatgacctt tgtattttta atactctgtc ttattggtat ctcgtggatt tttagaactg
     2641 ggtataagat gaaggcgtaa aatgaaaagt cattaactaa gacaattgaa ttcatacctc
     2701 atgcggatca aactcaagcg gacatcctct gcatatgggt gacttttttt tacagtgctc
     2761 ttttgctgct ttgactaata gtgcatggta ttcattgaat aactggtgat catccggtag
     2821 tgaattcata aataattcct gcatttctgt atagctcgta tattccggca ctaaaccgtg
     2881 gcggcttaga attctgtggg tataagcatc aataacaaag attggttttt tggctgcgta
     2941 aaggattata ctgtcggcag tctcgggtcc tatgcctttt atgctcagca gtttttgcct
     3001 aagatcatat aattgctctg aaaacatatc acccagattt ccagagtaat tatcatttat
     3061 aaacgatata aaagatttaa tcttgatagc tttttgattg tagtatccag atgaccttat
     3121 caactgagct aactgttcag cctggatgca agttagcttt tctatcgata tcaattcatg
     3181 ttgttttaaa ttgccgattg ctttttcaac atttttccat gttgtatttt gtgtaagtat
     3241 agctccgatt atacattcaa gctctgtctc agcaggccac cagttttgcg ggccataacg
     3301 ttcatttaat gcggagtaaa tctgattaat tctggttttt gtgtccattt ttttgaaatt
     3361 gattataaca tcaattatga tctgagaatt gtagtattgt attaaaatag gaaaccatga
     3421 aaaaaactgc tctatatgat aatcacaaga aaagcggcgg gagaattgta gaatttgctg
     3481 gctggcagat gccggtgcag tatgagggat tgaagcagga acatcttgca gtgagatcat
     3541 cagttggaat attcgatgta agccacatgg gtgaggttga gattaaaggc tctgatgcaa
     3601 aagagttctg ccaaagactt acttctaata acattaataa gattgtagat aatcaggctc
     3661 aatactcggt tatgtgtaat cataatggcg gagcagtcga tgatgttatt tcttacaaat
     3721 tttcagatga gcattttttt atctgcgtaa atgctgctaa tactgaaaaa gattataatt
     3781 ggattttaca gataaaagat gaatttaaat ttgagagcga agttattaat gcaagcatcg
     3841 agtattctca aatcgcaata caaggcccag atgcagcaag cttacttaca aaacttatgg
     3901 gacaagaaat ttcttctata aagagatttc gttttgataa gataaattgg aatggtacag
     3961 aatcaatcat tgcaagaaca ggctacacag gcgaagacgg gtatgaagtg tttttaccat
     4021 ggaaaaaagg gcctgcacta tgggatgagc tattaaatct gggaaatgaa tttggcataa
     4081 aagaatgcgg tcttggatgc agggatactt tgagacttga gatgggatat cctctttatg
     4141 gcaatgagtt aaacgatacc acaaatccca ttgaatcagg gcttgggttt tttgttaaac
     4201 ataaagatga agataatgat tttgttggca ggaaatccat actggataag attgaaagtg
     4261 gtttaaaact caagcttgtt ggatttgaaa tgacagaccg cggtattgca aggcatggtt
     4321 acaatatcat ccatggtagc gaggttgtgg gggttgttac aagcggcaca cattcgccaa
     4381 gtctggataa gtcaatcgga atgggattaa ttaattatca ttgtgaagaa aataatttat
     4441 gtgttgacat taggaataaa tatagaaaaa ttgaaatagt agatattcca ttttataaaa
     4501 aataaaaatt aaggagaatt tactgtgttg atacccgaag atcttaaata tacaagtgag
     4561 catgaatggg ttagggaaga gagtgagctg gtagttgtgg gaataaccga ttatgctcag
     4621 gaggctttag gggagcttgt atttatcgaa cttccaagcg aaggtgatga gataaacaaa
     4681 ggtgatacat ttggcgctgt agagtctact aaatcagttt ctgatctgtt tgccccaatc
     4741 agtggagagg ttattgaaat aaatgaagtt cttcttgatt cacctgaact catcaatcag
     4801 gatccatatg gtgagggttg gcttataaaa gtaaaaccgt ttgaagatgc tgatctttca
     4861 gattgtctgg attttgaaga ttataccgat attgtagagc aggagctgga aaaatagcag
     4921 ttttcttagc atttgttctc ttaaatttga atttaagata aaggttatta taattccctt
     4981 cttttaaata aaatccctgt atttaaataa tatttctgat ctgttatgga ctatccatta
     5041 gatagctttg tacaccgtca tataggcccg gatagcaagg atattaaaga aatgcttagc
     5101 actgtgggct gcagttcact cgatgaacta atcgaccaga cagttcctca aaatataaga
     5161 agcaataaac ccctgaaaat atctgctgca gagactgaat ttgattatct gagtaatcta
     5221 aggtcaatat cgcaaaaaaa caaggtttat aaatcatata tagggcttgg ttattacgat
     5281 acaataactc cggcagtaat attaagaaac atatttgaaa acccgggttg gtatacacct
     5341 tacactccat atcaggcgga acttgcacag ggaaggcttg aggctttaat aaactttcag
     5401 accatggtaa tcgactttac aggtatggag attgccaatg cctcgctttt agacgaagca
     5461 acggctgctg cagaagccat gacaatggct gcaaggttaa gggacagaaa gtccgaagaa
     5521 caagggcaga acaaattcct ggtttcagat aagtgtttcc cgcagactat agatgtttta
     5581 aagacaagag cagagcctct tgatatagag ttagttattt cagatataaa tcaagcagag
     5641 ctgagcgaac agtattttgg aataatatta cagtaccctg atcagtttgg gcaggtagaa
     5701 gattattctc aaatcataca aaatgccaaa gatttagata ttaagataat atttgctgcc
     5761 gatcttctca gtcttacatt actgaaggct ccgggtgaga tgggggcgga tattgtagtt
     5821 ggatctacac agaggtttgg tgtacctatg gggtttggcg ggccgcatgc aggatatttt
     5881 gccacaaaag aggaaaataa aaggcatatt ccgggaagaa taatcggagt atcggttgat
     5941 tcaaacggca ggagcgcata caggatgtct cttcaaacaa gggagcagca tataagaaga
     6001 gataaggcta catccaatat atgcacggca caagcacttc ccgcagtcct tgccggtatg
     6061 tacgccgtat accacggccc tgatggtttg agagcaattg caaataaaat tcacagtctc
     6121 acaagactgc tagataataa tcttaaagaa ttaggatttg tacagaacaa tgaacattat
     6181 tttgatacat taacagtcga tttatcaaac cactctgcat caattctaga tgaattaaaa
     6241 aagcagtgca taaaaaataa aattaacctc agatacatag atgaaaatac tattggaatt
     6301 tcagttgatg agaccaagag tgttaaggat attaaaaaac ttataaacat cttttcaagc
     6361 tcaatcggta agcagtcaga ttctgcttct tcagatgatg atttgaatag agtaaatcct
     6421 aaaattcctg agaacctgca aagaaacagt gcctacttaa ctcaccctat atttaatact
     6481 aaccgctctg aaacacagat gatgcgttat ataaagaagc ttgaaaacag agacctatcc
     6541 ctcaatacat ctatgacccc gcttggctca tgcactatga agctaaatgc tgcatccgag
     6601 atgattccgg ttacatggga agaattttca gcaattcatc catttgctcc tgctgatcag
     6661 acaaaagggt atgcgcagat tattaaagaa ctgcaaaagg atctaataga gataacgggg
     6721 tttaagggag tgtctttaca gccaaactca ggcgctcagg gagagtatgc agggcttatg
     6781 gtaataaggg cctaccataa ggacaggggt gaggaaaata ggaatatcgt tcttgttcca
     6841 agctcagctc atggtacaaa ccctgcaagt gcggttatgt gcggtatgaa tgtggttgtc
     6901 gtaaagtgtg ataatgatgg aaatatagat ataaatgacc taaagaaaaa agcatctgct
     6961 cataagagtg aacttgctgc aataatgatt acctatcctt ctacccatgg agtgtttgaa
     7021 gagaccatca aacaggtctg caaggttgtg catagaaacg gcgggcttgt ttatatggat
     7081 ggggcgaaca tgaatgctca ggttggactt acttatccgg gtacgtttgg agtggacgtg
     7141 tgccatttaa accttcataa aacatttagt attccacatg gcggaggcgg tcccgggatg
     7201 gggccgatat gtgtgggcag gaaactacta aaatatcttc cgggtcatgt gtatgctagt
     7261 gttggcgggc ctaaagccat aagtgcagta agcgctaccc catggggaag cggcagtata
     7321 cttttaatat cttatggata tataaagatg cttggggctg caggggctac tgaggctact
     7381 aaaattgcgg tattaaatgc taattatata aagtccagat tgggtaataa ttacaaaata
     7441 ttatatcagg gtaaaaacgg cagagtcgcc cacgaattaa ttattgacct tcgtgagttt
     7501 aaaagatcaa ccgatattga agtagaagat atagcaaaga ggcttatgga ctatggattc
     7561 catgcaccta cggtttcatg gcctgttgcc ggcaccatga tgatagaacc aactgagagt
     7621 gagccaaaag aagagctgga taggttttgt gatgccctga tatcaattta tgaagagata
     7681 aacgatatag ctaggggtaa gtacgaaaaa gataataatg ttcttaagaa cgctccgcat
     7741 acagctgcgt atgtaagttc tgatgagtgg agcaattcat attccagaga gcttgcagca
     7801 tatcctgaag cttatctgag ggaaaacaag ttctggcccc ctgttggcag gataaataat
     7861 ccctatggag ataaaaatct tgtttgtacc tgtattgctg tagaggatta tgagtaaggc
     7921 tttgatttta aggctaaatc aagttacagc ttgatttcta tataagcgtt ttctctcata
     7981 gtagcaatat atttttgtaa ttcttcagct acttttttct tgtaaatttc ctgtcttagc
     8041 tgttctctat aggtgtctac tgaattacta tcaagtgtgt tcctgttgag tacttttatt
     8101 atgtgaaaac ctgaagccgt ttgaacaggt ccggatacct ccccaacttc agttatggct
     8161 actacagctt ctagttctga tacaagctca ccttttttaa aataacccag tacaccttct
     8221 ttttcttttg aaaatgtatc gtcggaatat tcagctacaa gtttgctgaa atcgtttcct
     8281 gattttgcaa gctctgctat gcggctggct ttttcaagcg cccgctcagg ctgctgttca
     8341 ttaacagata tgagaatatg agctatttgt acttcatctg agttttctat gttgccataa
     8401 tattctttat agagattttc tatttcatca tctgtaactg caactcttcc cttaactata
     8461 gagtttacca attttccgct tattatttgc agacgccact gttttctgaa ctgttctttt
     8521 gtgagatttt cttttttgag catttcttcc atttgttcat ggctcatccc ggcttttgtt
     8581 cttatatttt caattgacat atcaatttcc ggatcagaaa cagtaatacc caatttggcg
     8641 gcttcctgct caagtatcat ctgttctact aaatcattta gtacacggtc tctgtcaatt
     8701 tcagctgtac ctggaggaat agagcttata tatctattga gatcggatag agtaataaga
     8761 tcatcgttta ctacagcaac gattctgtct attacttcag tggcataaca aataccagtt
     8821 atcattatta taagccctgt aattatagtt gttctaattt tctgcattac tatttgtctc
     8881 cctggtttct ttataactat aaatttctat gttttctaaa tctttaagat ttttcagata
     8941 gcttttaaac ctattatttt tgacctcaaa ttttatatta tttttcaggt ttttaaatgg
     9001 tatatgattt ggtccatcta taagttttaa aataacataa gaatttccaa attttacagg
     9061 ctctgaaatc tctccttttt tcatattaaa ggctacatca cccaagagtt tgctgtattt
     9121 atctctggta atttctatat tttttttaac cttgattttg tagttaatgt gatttgttaa
     9181 accaccgagg tctgcatctt cttttagatt tgatattatc tcattttgca gatttagtag
     9241 ttcgtcctga ttgagatttt ctatatcttt ggggatataa gaaacctgag ttatggtggc
     9301 actctggtat aaatttttat tactctcata atactgttca agctctttgt ctgttatttc
     9361 ttgatttaga atatcctcac ttaactttct aatcattaat ttcttcttgt attgctcaac
     9421 tgattttctt atatcttctt tttcaccgta tccttttttt agggcttctt tgtataatat
     9481 ttctttgttt atatatgatt ctataaactt gtttttttgc tttggagatg actgtatgag
     9541 tattttttgt ttcaccggaa gtgcggatat ggcatcctgg agctgctttt ctgtaatatg
     9601 atcagaacca attttagcga caatattgtc tacaacaggt tttttggttt ttacactctg
     9661 cttatcagaa ctatcatgac aagatacaat aattaaagcg cttaaaagaa taagtgtttt
     9721 tatcatattc acttaatgtg ctgtcttatt agttggttga ttcatatacg gtggattttt
     9781 agaattaaat gaagacataa tttcttttac agattttaat gttaactcac ttttttcgct
     9841 tattctatat atgccggatg gtctgaaata tttaaatagc gatgagtttt ccaaaaactc
     9901 cattaccaca ctgtttttct taagctcaag ctttttgatc aaaaactttt tcatgtagat
     9961 ttttatttct attaaactaa atagattaac aacagtttcg ggagccctgc caaatctgtc
    10021 cgatatatca gaatatatct cttttaattc ttttaaactc ctgcatgatg atagtttttt
    10081 ataaaccata agcctctcgg aatcgtcttc tatatattca tttggaataa atgtggggta
    10141 agaagattta atctcaggct caaaactaaa ctcctgtttt tgattgctca ggtttcttac
    10201 ggcactttct aacatttcca gatacatttc tagacctata tcaccaatat ggcccgattg
    10261 ttcagtgcca aaaagcgtac cagcgcccct gatttccaaa tcagatagtg caagcttaaa
    10321 tcctgaaccc agttctttta actcagaaat agccttgagc ctctttttag aactttgcgt
    10381 taatttctct gaacccggga caagaaaata ggcataagct tttttatcag ctctgcccac
    10441 tcttcctctc aactgataca ggtcggcaag tccaaacata tgagcatcat ttataataat
    10501 cgtgtttgcc cttgatatat caagccccga ttctacaatt gtggttgtaa tcagcagatc
    10561 gatatcaccg ttaataaatt tatttatact attttccaat acagattctc tcattcttcc
    10621 gtgtgtgatc tcatatttca catcgggaac tatgttggta attctctttg caattgtgtg
    10681 aatatcttca atcctgttgt gaataaaaaa tactgctcca cctcttttta gctcgtttgt
    10741 tatagcttcc cttatggtgt tttcattgaa atgatataca tatgtttcaa tctgctgtct
    10801 tccggcagga ggggtgttaa taaggcttat atcccttatg tttgctaaag agagttgcag
    10861 ggttctagga atcggcgtag cgctcagggt tagtacatct actgaggttt tgatctttct
    10921 tatattttct ttttgtttaa cgccaaattt atgttcttca tcaactatta tcaggcctaa
    10981 gtctttaaaa tgaactttac tgccaagtag tttatgagtg ccgattatga tgtcggtatt
    11041 acctaagtac agagattcca gtattttatt tgtttgagcg gaggttctaa atcttgaaat
    11101 catttcaaca tttatcggaa aacccttaag gcgtgattta aaagtaagat agtgctgaaa
    11161 acaaagaagt gtagttggta ctattactgc tacctgtttg ccgtccataa cggccttgaa
    11221 tgctgctctt atcgctacct cggtttttcc aaaacccaca tcaccacaga tgagcctgtc
    11281 catgcttaaa tcagattcca tatccagcat cacatcgtta atggcactta cctgatgagc
    11341 ggtttcctca tgctcaaacg actgctcaag ctcagagtag agcgtatcat ttttagaaaa
    11401 tctaaaaccc ttttcggcct ttcttttagc atagagctgc aggaggtctt tggcgataag
    11461 ctcaatagat ttttttactt tcttaagagt ctttgcccag gtttctttgc caagtttttc
    11521 aatggttgga gattttccct caccaacata cctttgaact atgttgagtt tatcaacggg
    11581 aacataaatt ttatctccgt ctttatactc acatactaaa aaatcagcct ttttaccttt
    11641 tatatcaagt tgtaatagac ctctgtatat gcctacaccg aaatccttat gaactatata
    11701 atcaccaggt tttaattcac taaaggaagt tataaatgcc gatggaatgt catcattttt
    11761 ggaaaccggc tttacctttt tttgatggta tatctcattt tcggttatta cggcaatccc
    11821 aaattcttca gatacgaaac cactttcaat ttcacctatc tcaaattgaa tatcttttag
    11881 ttttctggac tttattattt ctgctagttt ttcggtttca ccctttgtct ttagaacaaa
    11941 caagatttta taatcttctt tttgtaattg cttcaatctg tcagcaagct cattataagg
    12001 tgaggggctg ttttcaggct gcttatgaaa atcaatattc ttaaagttca gattgatgct
    12061 gtttttaccg cttacaggta actcaggaat atagattttt ttaatttgtg ataactttgt
    12121 ctttaattca ggcaggctca aatataaaga gtctaaatca gggataagct tgtatttctt
    12181 ttttagtact aacagttttt cagaaatatc ttccaaacac tgttcaaagt acttttcttc
    12241 acttcctgaa taaaagaata acaaagtgtc ttcattcata taatcaaaaa tgctttttaa
    12301 agatttaaaa caaaaaggga taagccagtt tatatattct ggcctttgct cattttgcag
    12361 ctggtgtata agtgagtttc tataagaagc ggatactcca ttttcattgg aaaactcctt
    12421 aatcgatgat tttatatagt taatattgtc ttggtcaact atgagttcag tagttgggat
    12481 tacgctggct ttatctgttt tgcttactgt cttttggttt actgtatcaa agaaccttac
    12541 cgagtgaatg ctgtctccaa agaactcaat tcttaaagga agttcatagc atggtgagaa
    12601 tatatctatg attaatcccc tgtggctgta atcacctttt ttctgcacaa aatctgtttt
    12661 tgtataccca aaagcactga gcctttgctg tagcagtgtt ctatcgagtt tatcatttat
    12721 atttatcttg atactgcagg tatcataaaa atcccttgga ggatattttt caaggagagt
    12781 ttgtatatca caaacaagaa ttttgtggtt ttgagcatca taaagccagt tacttctgag
    12841 actaaacaca tctgagctgg atgtaaaaag aggtctatct gcatcaagtt cttttttagg
    12901 taagtaaaga actttggaat tagttagata tgatagctct ttttgggttt gttctgcctg
    12961 tttatcatcg gtagtaataa tgagaagctg cttttgcgat ataaggctta gccatgaagc
    13021 aaaaaagttt tttgaaaaac cttgcagacc acccagatat accttgtcat tgctatctag
    13081 agtatcaaca atgtgccggg caatatactt gttgttaacc ctggtgctta ggtgttctat
    13141 tatagattgc ttattcgttg acacttcctg caattaataa ttattttgta aatctttgag
    13201 aattaaatca cccatttccg tacagttaac cttctttgag ccctgttcat aaatatctcc
    13261 tgttctatag ccactttcta gcacactgtc cacggagctt tgaattttat ttgaaataga
    13321 atcaaggttt agtgaatact tcagcatcat agctgtagta agtatcgacg ctattggatt
    13381 tgcaagattt tgccctgcta tatccggtgc gcttccgtgt acaggttcat atattgcgcc
    13441 gtgagtaccg atacttgcag agggaagcat gccaagtgat cctgtaagct gagctgcttc
    13501 atcgcttata atatctccaa acatatttcc tgcaagcata acatcaaagc tcttgggtct
    13561 tctgataagc tgcatggccg cgttatccac atataaatgt tcaagctcaa tatcagcaaa
    13621 ttctgtttct tttacttcag ttaccgtatc tctccacagc tgcatgactt ccagcacatt
    13681 tgctttatca atcgaggtta cttttttctt tctcttgcct gcaatttcaa atgcaactct
    13741 tgctattctt tttatttcgc tggttgtgta gctaagtgta ttggtgcctt ttttctcatc
    13801 gctatccagg tattctatgc cccgcggagt tccaaaatat atccctccgg taagctctct
    13861 tactaccatt atatcaaccc cgtctacaat ctcatttttc agggtagatg cgttttttaa
    13921 tgggccaaaa accacggcgg gtcttaggtt tgcaaaggca tcgagctctt ttcttattct
    13981 cagcaaccct cgctcgggtc ttatctcatg aggcaggctg tcccacttag gtcctcccac
    14041 tgcacccata agcacagcat ccgattccat tgcaatttct attgttttat ctgtaatagg
    14101 aatcttgtgt tcatcaagac atgcgccgcc aaataaagct tcttcaaatt ccagtccaag
    14161 gttttcctgt tcatttaaaa aattgaggat ttttatactt tcatttgtaa cttcggttcc
    14221 tataccatcg cctggcagta aaagaatttt aggcatatat tctgttctcc tgtttttagt
    14281 aattaaaact gcgtatttta cctgaaataa aaaattagat ggaattgtta gttttattat
    14341 ggttaaaata tatgccagac ataacaaatc aatttaagat ctaaaaaatt ggcaaagaaa
    14401 aaaagagcta aaaatactaa gaacaaggat gtaggtacat cagatgtaaa gactcaagtt
    14461 gagtctgagc aattagatga gttaacccta aagctgccta gttggctgag gggtgaggtt
    14521 tttctttcta tactgttttt tgtccttgca tttgtactct attctttttc actaaaaaat
    14581 gagtttgttt gggatgatgt ggaggttatt aagaaaagct actataattt tgaaaatacc
    14641 agtttaattt caaaatttat tcctgctgta aatgaaacga aaaaactgct ctactacagg
    14701 ccaaccatac caatatcata tataattgac tggaataact ggaatttaaa cccatttgga
    14761 tatcacctta gcaattccat tttttatgcc tttactgttg taggcatgtt tttcttatca
    14821 aacagaataa ccaggctttt ttcaattaaa caggcactta ctttttccat gcttgctacc
    14881 ctgctctttg tactccaccc aatgcacgta gagtctgtat catggattgc gggcaggact
    14941 gatgttttgt gtactatgtt tttcctgttt gctctgggat ttcatatatt aagctacaag
    15001 aaattttatc tgctgccagt agtatgtttg ttttatttat tttcactatt ttcaaaagaa
    15061 gtggcagttg catttccgct cttgataata tgtatggatt atttggtgaa aagacctatt
    15121 acaaaagcag atttatttag gtacgtggta tattttagtc tcactggttt gtatttatat
    15181 ctgcgttcaa gatcttttat caatataccc caagtatata atcctgatat tgtaagtgat
    15241 tctcaaagtc ctggccaatt actatattat tttggaatat ttaaggtatt tattgtctcc
    15301 tatgcttact atttaaagca attacttttt ccctacacat ttaacgcctt tgtaacctac
    15361 ctgccgaccc aggttttata cttaacagtt tctataattt tgattgctat attggtaata
    15421 tttttctttt attcttattt aagaaataaa aaatttatta catttaatat cttttgggtc
    15481 tttttaaccc tgggtccatc tgtactaatt gcaatatttg catttatcgc tactccagtt
    15541 tctgaaaggt atctgttcct gcctactgta ggatactgta tgcttgtagc ttatctactt
    15601 actgggtttt tggataaacc acaaacaagg actgtatcac tggtaattac atgttttctt
    15661 atcggcagtt ttatgttttt tactatcgac agacagagcg tctggcaaag caggcttaat
    15721 ttttggcagg atgcctccag caagtcggat catcatgcaa taccccatat aaattatgga
    15781 atggctcttt tagatgcggg aagaactgac gaagcaatca gtgaatttaa tataagctta
    15841 aaaccggaca tacagaatac tcccacagga agagctattg cagcaaataa tctgggggtt
    15901 gcttatatta ataaaaataa ttatgagcag gccgcaagaa ctcttctcat cgcactggaa
    15961 agtgatccgg gcttttataa gacatactat cattttggtt tgttgtatta tatcagagca
    16021 ggtaagtcca agcttgaaga tgattttgct acaagtgaga ggtttttact caaggcaaca
    16081 tctatttatc ctagatatgg aaaagcatac ctcttgcttt ccaagctaaa cagtaatttt
    16141 ggaaataagg aaaaagcagt tcattatgca acaaaagccc tgcaaagcgg tctggtaaac
    16201 gaacttgctg cccaggctac aaaagttatt cagatgaaca agtgagtgtt cagtatcaga
    16261 aaatataatt ttatctgttt tgcatattta aatacaaatg gaaacaaata atttacttcc
    16321 atctataata aaaaacagac ttattgcatc actgttttta ttccttgttt cgtttgcagt
    16381 ttttatccct tcattaaata atgattttgt ctgggatgat ataccatatt tgcttaacag
    16441 gatcgaacat ttaacaattt ctaaacagat aaataaagtt attcccagaa taaaaaccaa
    16501 ttccaataat aaatcagatt attacagacc catgctacat atttccctgg cattggataa
    16561 tgacatatgg aacggcaaat cacttgggtt tcatctctca aatatagtaa tgcacagcat
    16621 aacaacagta t
//
LOCUS       DBNGSV010000046         2691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_422946, whole genome shotgun sequence.
ACCESSION   DBNGSV010000046 DBNGSV010000000
VERSION     DBNGSV010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_422946"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..716)
                     /locus_tag="AC159U_01705"
     CDS             complement(171..716)
                     /locus_tag="AC159U_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3335804.1"
                     /translation="MTQLILIIAAIFLLSLFFGISKSLSALRKMSEQYWGNVQSQLFR
                     KYDLISVLSDFIKNHTDIDKDTEDKLTEAKLKAMQSYSPFQKSMTEKPLDDCIEDILK
                     ITEADEKLATDEKFQRIRDDISEVQKELLMSSSYYNAIIRDYNSKISSFPSSFVARLF
                     GHYSKETFDLEINLQSLNKKR"
     gene            complement(813..2033)
                     /locus_tag="AC159U_01710"
     CDS             complement(813..2033)
                     /locus_tag="AC159U_01710"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR3335805.1"
                     /translation="MATISGFHGYRYNPEVIRNFDEVLAPPYDVISSEQRDELYEKSI
                     NNVVRIILTKGEGDAKYKEAADTFNNWIKDKVLIRDEEPSIYVYQQVFEEGGKTYKRK
                     GFISAVKIEDFENKQIFAHERTFSHHKADRLKLITASKANLSPVFSVFSDPDHVINLA
                     LKNSLFDYPLFKSTDNDGVENYLWKVSDSDLLSFLAEKFQEKKLLIADGHHRYETAIN
                     YRNLRREQNPEGNGNEPYEYVMMFLASAEQPGLIVKPTYRLIGNLTEDRASEILGKIE
                     NDFDGEKLDIKTGINKIGKSEYGVVTQGLDKLSRIKDLKGNESSELGVVKLHDQLLDS
                     ILEDESVSYKYTKSLDEVMELLKDKTYQLGFILPEIDAVDILEFAESGRRLPQKTTYF
                     YPKVLSGLVFNLLD"
     gene            complement(2052..2678)
                     /locus_tag="AC159U_01715"
     CDS             complement(2052..2678)
                     /locus_tag="AC159U_01715"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3335806.1"
                     /translation="MSAKKVIKADSPDAVKRTVEILSSGGVIVYPTDTLYGLGALVSK
                     EQAVQRIFEIKGRKPEMSLPVLAKDFKMLQDYAQVPEKYKEMLQKYMPGPFMAILKLK
                     QYLNPIITGGRDTIGIRISNNNFVIQLMENLDEPLISTSANLSGTGNMYDISQIISVF
                     GNMVDLVIDSGSISASKGSTIVDFTLTPPEVLREGDLEKARLAKYLTN"
BASE COUNT          795 a          539 c          422 g          935 t
ORIGIN      
        1 agcaatgggc ggcatgatgt agtctctatg agacagatat aacacttgtt aaacaagtaa
       61 gataatttat ttaaaatcaa acagggcggg ttactattta tttaagtagc ccgccttttt
      121 ttatctaaaa tttatattgt aaacgaggta tttagcagca aaggaatctg ctaccttttt
      181 ttatttaagg actgcaggtt tatctcaaga tcgaatgttt ctttactata gtgaccgaat
      241 aaccttgcta caaaactcga tgggaatgaa cttattttgg aattataatc tctaataatt
      301 gcattatagt aactggaaga catcaagagt tctttttgta cttcacttat atcgtctctt
      361 atcctttgaa atttctcatc ggtggcaagt ttttcatcgg cttcagttat tttaagaata
      421 tcttcaatgc aatcgtccag aggcttctct gtcatggatt tttgaaacgg cgaataagac
      481 tgcatagcct tcaacttggc ttcagttaat ttatcttctg tgtctttatc tatatctgta
      541 tggtttttga taaaatcgct aagaactgaa ataaggtcat actttctaaa aagttgtgac
      601 tgtacgttcc cccagtactg ctctgacatt ttcctaagag ctgagagaga tttagaaata
      661 ccaaaaaata ggctaagcaa aaagattgca gctattatta atattaattg tgtcacttaa
      721 tcatcatcca aacgtaagat atatatctaa taaaatatat atttatttca gaaaaaatct
      781 attaaaaaaa gagttttatc taatatctcg ttctaatcca gcagattaaa aacaagtcct
      841 gataaaactt ttggatagaa atatgtggtc ttttgaggaa gtcttctgcc agactctgca
      901 aattcaagaa tatcaactgc atctatttca ggaagtataa acccaagctg atatgtcttg
      961 tcttttaaaa gctccattac ttcatcaaga gatttcgtat acttataact aacagattca
     1021 tcttcgagta tagaatcaag cagttgatca tgaagtttaa caactccaag ctcactactt
     1081 tcattgcctt ttaaatcttt gattctacta agtttatcaa gcccctgagt cactactcca
     1141 tactcgctct ttcctatttt attaatacct gttttaatgt ccagtttctc accatcaaaa
     1201 tcattttcaa tttttccaag aatttcagaa gctcggtctt ctgttagatt tccaattagc
     1261 ctgtatgttg gttttactat aagccccggc tgctcagctg aagccaggaa catcattaca
     1321 tattcgtatg gttcatttcc atttccttca gggttttgtt ctcttctgag attcctgtag
     1381 ttaatagcag tctcatacct gtgatgacca tctgctataa gaagcttctt ttcctggaac
     1441 ttttctgcaa gaaacgatag cagatcagaa tcggatactt tccacaaata attttccacc
     1501 ccgtcattat ccgtactctt aaacagcgga taatcaaaca aagaattttt aagcgccaga
     1561 ttgatcacat gatcagggtc agaaaagact gaaaacactg ggctcaggtt tgcctttgaa
     1621 gccgtaatga gctttagtct gtcagcctta tggtgggaga atgttctttc atgcgcaaag
     1681 atttgtttgt tttcaaaatc ttctatttta acagccgata taaaaccttt tcttttatag
     1741 gttttacctc cttcttcaaa aacctgttgg tagacgtaga ttgaaggctc ctcgtctctt
     1801 atcagcacct tgtctttgat ccagttgtta aatgtatctg cggcttcttt gtactttgca
     1861 tctccttcac cctttgtaag gatgattctt actacattat ttatactctt ttcatacaac
     1921 tcatccctct gctcagagct tataacatcg tatggaggag caagaacttc atcaaaattc
     1981 cttataactt cggggttata tctgtagcca tgaaaaccgg atattgttgc cataatttaa
     2041 gagattctcc tttaattcgt aagatatttt gctaatcgag ccttttcaag atcaccctct
     2101 cttaagacct ccggaggggt tagagtaaaa tccacaatag tagaaccttt agaagcgctt
     2161 atactaccag agtcgattac aagatcaacc atattgccaa aaacacttat tatctgggag
     2221 atatcgtaca tattaccagt cccgctcaaa tttgcgcttg tggaaataag gggttcatca
     2281 agattttcca ttaactgaat aacaaaatta ttattagaaa ttctaatccc aatcgtgtcc
     2341 cttccgcctg taattatcgg atttaaatat tgttttagtt tcaaaatcgc cataaaaggc
     2401 cctggcatat atttttgtaa catttcttta tacttctcag gtacctgagc atagtcctga
     2461 agcatcttaa aatcttttgc aagcacaggt agagacattt caggctttct gccttttatt
     2521 tcaaatatcc tctgcactgc ctgctctttg gatactagcg caccaagtcc gtacaaagta
     2581 tcagtgggat acactatcac tccaccgctg ctaagtattt caactgtcct ttttacagca
     2641 tccggactgt ctgcttttat cactttttta gcactcatat aactttatac c
//
LOCUS       DBNGSV010000047         3377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1116157, whole genome shotgun sequence.
ACCESSION   DBNGSV010000047 DBNGSV010000000
VERSION     DBNGSV010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1116157"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC159U_01720"
     CDS             <1..384
                     /locus_tag="AC159U_01720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3335807.1"
                     /translation="ALKNSKLNPSDVDYINAHGTSTQQNDINETNAIKQVFNGNSVKI
                     PVSSTKSMTGHLLGAAGGIEAGITSLSLYNGIVPPTINLDNPDEGCDLDYVPHEVREK
                     DIKVAISNSFGFGGTNVTLAFKKFE"
     gene            683..1243
                     /locus_tag="AC159U_01725"
     CDS             683..1243
                     /locus_tag="AC159U_01725"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3335808.1"
                     /translation="MVSILGPENEKETGMFTQGYWVMPIAKYVEKYGINHFKESIDAI
                     KEITKRSTSEYAIRPFIEKYPERTIKVMEKWSKDRNVHVRRLSSEGMRPRLPWAPKLQ
                     IFIENPNPIIPILENLKDDPSKFVQKSVANNLNDILKDNYNIGIDTLTKWSRRATTER
                     KWIIKHALRNQIKKKNPEALYIASQI"
     gene            1275..1550
                     /locus_tag="AC159U_01730"
     CDS             1275..1550
                     /locus_tag="AC159U_01730"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR3335809.1"
                     /translation="MKYIVTWNIKPENYKEAVEIFLQNGAQAPDGVTILGRWHALGSN
                     HGWVLVEGDETGIATHIAEWSNLLELSITPVFDDEQAAASLSKVYGN"
     gene            1594..2463
                     /locus_tag="AC159U_01735"
     CDS             1594..2463
                     /locus_tag="AC159U_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3335810.1"
                     /translation="MNLPRNKNLGYSSAVISTVLLGSVGIFVRNIQVDELIITIARLG
                     IGLLFLIVYLLITRRLPKIELKKFTFPLIATGLLLGLTMLFYMKAINITTLSNAVFLL
                     YLGPIIAAAITSIVFKERISYLNILLICIAFLGFLFLLEFKFNLSGSMGNVLAFAAGL
                     CYASYIVLNRRISTHVDSMERSFYQFLFGLVILLPFIITVENINFNLRDIYWLTGISF
                     FQGFLAITFIIIAIRNLKAIEYGTISYVEPLVASLIGYMLYSEQLSTLQLIGCAIIFL
                     SGLTQVVTSNKNI"
     gene            2541..3377
                     /locus_tag="AC159U_01740"
                     /pseudo
     CDS             2541..3377
                     /locus_tag="AC159U_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         1137 a          509 c          568 g         1163 t
ORIGIN      
        1 gcacttaaaa attctaaact aaatccatct gatgttgatt atattaatgc gcacggcact
       61 tctacgcaac aaaatgatat aaatgaaaca aatgctatta agcaggtttt taacggcaat
      121 tccgttaaaa tcccagtcag ttcaacaaaa tccatgacag gacaccttct tggtgctgcc
      181 ggaggcatag aagcaggtat tacttcttta tctttataca atggaatagt gcctcctact
      241 ataaacctag ataatcctga tgaaggatgt gatcttgatt atgtaccgca tgaagtaaga
      301 gaaaaggaca ttaaggtagc aatcagtaat tcatttggtt ttggtggaac taacgttaca
      361 ttggcattta agaagtttga ataattatgt aacatatata agtatattta tttttatcac
      421 cggttattca cctatacatt cataaataat ggatgggtgt gtactcatgc ccaataaaaa
      481 attaaaattc tggtttgaca aggaattagc aattttattg gctcataaaa taacaccact
      541 atacccatag tttgattcta aatcttttat tcaagatgtt gatagcagaa cgagtgtact
      601 ggaactaaag gacagagtcg aacttattgc agatctttta aatgatcttc tttacctaaa
      661 ttacaaagat tctgttgaaa tattggtaag cattctaggc cctgaaaatg aaaaagagac
      721 aggaatgttt actcaaggat actgggttat gcccattgct aaatatgtag aaaaatatgg
      781 aattaatcat tttaaagaat ctatcgatgc cattaaggaa attaccaaaa gaagtaccag
      841 tgaatatgct ataaggcctt ttatagaaaa gtatcctgaa agaactatca aggttatgga
      901 aaaatggtca aaggatagaa atgttcatgt tagaagatta tcaagcgagg gcatgagacc
      961 aagactacca tgggcaccta aacttcaaat attcatagaa aatccaaatc ctattatccc
     1021 aattctcgag aacctgaaag atgatccatc caaatttgta caaaaatcgg tagctaacaa
     1081 tcttaatgat atccttaaag ataattataa tataggaata gatactctaa ctaaatggtc
     1141 tcgaagggct acgactgaaa gaaaatggat tattaaacat gcactaagaa atcagataaa
     1201 gaagaaaaat cctgaagcac tttatatagc ttcacaaata taaaagttga tacatatttt
     1261 ataggagata aatcatgaaa tacatagtga catggaatat caagccagaa aattataaag
     1321 aagctgtaga gatattcttg caaaatggag cgcaggcacc ggatggtgtt accattctag
     1381 gacgatggca cgctcttggg tcaaatcatg gatgggtttt ggttgaagga gatgagaccg
     1441 gcatagccac acacattgca gaatggtcta atttacttga actatcaata acaccggtat
     1501 tcgatgacga acaggctgca gcaagtttat caaaggtcta tggtaattaa ataatatttt
     1561 taaagtataa atagatgtgt tttaatacgg ggaatgaatt tgccccgtaa taagaattta
     1621 ggatattcat cagccgttat ctccacagta cttcttggct cagtaggtat atttgtaaga
     1681 aatattcagg tcgatgagtt aataataaca atagcaagac tgggtattgg actgctattt
     1741 ttaattgttt atcttttaat tacaagacga ctccctaaga tcgagcttaa gaaattcacc
     1801 tttcctttaa ttgccacagg tcttcttctt ggccttacaa tgctttttta tatgaaagcc
     1861 attaatatca ctactctttc aaatgcagtg tttcttttat atctgggccc tattatagcc
     1921 gcagcaataa cttcaattgt gttcaaagaa agaatcagtt atttaaatat attacttata
     1981 tgcatagcgt ttttaggatt tttattttta cttgagttta aatttaattt atccggttca
     2041 atgggaaatg tattagcctt tgcagctggg ctttgttatg catcttatat agtgttaaac
     2101 agaagaatat ctactcatgt agattctatg gaaagatcat tttatcaatt tctttttggt
     2161 cttgttatat tacttccatt tataattact gttgaaaata tcaattttaa tttacgcgac
     2221 atatattggc ttaccggtat ttcattcttt caaggttttc tcgcaatcac atttataatt
     2281 attgcaatca gaaatttaaa agccatagaa tacggaacaa tttcatatgt agaaccactg
     2341 gtggcatctt tgattggtta tatgctttat agtgaacaat tatcaactct acaattaata
     2401 ggttgtgcaa ttatatttct aagtggatta acacaagttg ttacttctaa taagaatatc
     2461 taacgtcata gtttattaat aatttatata actttttaaa aatcgtatca aaatagtttg
     2521 agatatgttt aaataatgtt gtgaaaatat ccagcaataa aaacttggga tatgccactt
     2581 caatatttgc ttcaatcctt cttggatgtg aagggatata tgtaagaaat atcaatgtag
     2641 atgaattcat aataactata gccaggcttg gtatagggtt aatatttata gcactttatt
     2701 tactggttag aaatcaattc cctaaaattg aatttaaaga tatttctttt ccactaatta
     2761 tttccggtct actgcttggt cttataatgt ttttttatat gcaggctata aatcttattc
     2821 cactttcaag tgctgtgttc cttttatatc tggggccaat tattgctgtt ggtataagtt
     2881 atttaatatt taaggaacct gccacaaagt taaactttat attaattgct ctagcatttg
     2941 tagggtttct ttgtctgtta gaattcaaaa tatcatttga tctttctaaa tcaaaagggg
     3001 ttctattggg agcagctgcc gggctttgtt atgctctata catagtacta aaccgaagaa
     3061 ttccaaataa tatagattct atgcacaggt cgttttatca gtttttattt ggatttataa
     3121 ttttgttacc atttctatat acagttgata gtttcagtat taaaccaaaa gatattttat
     3181 tgcttacagg tatgtcattt acccagggtt tttggcattt acatttatca ttgttgcaat
     3241 taagtattta aaagctgtag aatatggaac tatctcttat ctcgaaccgc ttgtagcaac
     3301 gttaataggc ttcttgcttt atcatgagca attgtcaatt tcgcaattat taggttgtgc
     3361 aattatattt acaggtg
//
LOCUS       DBNGSV010000048         9533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_908738, whole genome shotgun sequence.
ACCESSION   DBNGSV010000048 DBNGSV010000000
VERSION     DBNGSV010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_908738"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="panC"
                     /locus_tag="AC159U_01745"
     CDS             <1..819
                     /gene="panC"
                     /locus_tag="AC159U_01745"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3335811.1"
                     /translation="ADMKAFSESHRSQGNTISFVPTMGALHEGHLQLVKNARNLSDIL
                     VVSIFVNPTQFGPNEDFDKYPRDLDKDKNLLEELGVDAIFFPSADNMYPGGFQTTVEV
                     QEIQKPLCGKFRPGHFGGVVTVVLKLFNIVKPHYAVFGEKDYQQLCVIRQMVSDLNVD
                     VEVLSHPIVREESGLAMSSRNEYLKSDKRQQAASISDALFNVKKQFDSGVNDKKNLLD
                     TAYSILNEADIREIEYLEIVDGQNLSEKKAVSSGDVVAVAARLDGTRLIDNIRL"
     gene            835..1224
                     /gene="panD"
                     /locus_tag="AC159U_01750"
     CDS             835..1224
                     /gene="panD"
                     /locus_tag="AC159U_01750"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR3335812.1"
                     /translation="MHRTLLKSKIHRATVTDANVDYEGSVTIDRELMDLADLQEYEQV
                     HIYNITNGNRLSTYVIEGQRNSGHICINGAAAHLAKTGDLVIIANYADYSENEASLHK
                     PKLIYVNDDNSVKHISDHVAKLKVAGS"
     gene            1526..2320
                     /locus_tag="AC159U_01755"
     CDS             1526..2320
                     /locus_tag="AC159U_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR3335813.1"
                     /translation="MKAIILAAGRGKRLYPYTKYIPKCLLDVGGVTILEHQINSIRAC
                     GIDEVVIVIGFGFEKVEDFLRNYDGLGLKIKTLYNPFYQTTNSLISLWIARGEMDQDI
                     VVMNGDDVFELEVLDSALSMLEDKICLPAKKRDEYEDEDMKIVVKEGNIVEVGKSLES
                     SPSAESVGVRVFRDTGVELLKRSIEEEMRSENPEKKWYISAIHRLIEKGYKVKSFDVE
                     DMFWMDVDYPGDLFKARINASKFIDSKFQTDVPYEQRLLRVVDSNR"
     gene            2329..3639
                     /locus_tag="AC159U_01760"
     CDS             2329..3639
                     /locus_tag="AC159U_01760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3335814.1"
                     /translation="MYHAIIVASGTGIDGADLSEFPTKELVGVPQLNRLIIVAQRAGI
                     SKFTVLTQTDNYHALDNIETDKRISSSVNWHKKGEKISLDDEHCLVLQSNLVITPESL
                     KDFINQKIAKNDFLILVDELDNPSLTLNNGKIEGSFKNSGQAIGAFISTGKELKKIIN
                     SSDSIGSFVSNKLSSSAKIKYQQITSGYWYHLNDTDQSFNEAKEILFSNVGKTATGWV
                     SRKINSRFSLPTSRQLVKTSLTPNMISVLLNVIGMMSGVFLALGHPVWGCFFLYVATV
                     LDRCDGEVARVKLMETKTGQWVDTISDQFTVLSFFIGLTIGYYRVSDSNLAIFFGLFN
                     LFVFFFFLIWSFHYLSKYTNSGSLVEYFTIDKYEDNPSFIRRFILFLRPLSRRNAYAM
                     GFMIVSIIGGYTWMFGLLTFAMLLFFIHLIEDYLNIKNYSNQKG"
     gene            3649..4401
                     /locus_tag="AC159U_01765"
     CDS             3649..4401
                     /locus_tag="AC159U_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR3335815.1"
                     /translation="MSNDNTSKGFNPPKKYAGGLSSFINWAEGINERYSINGNPPVYD
                     NSSFPWVKEVEDEWLSIRSELEDVMKHRSELPNFQNIMPGVDAITTDNNWKTYFLAGY
                     GIESKENCKRCPETARILKKIPGMKTAFFSILSPNKHIPAHKGPFNGVLRYHLGLIVP
                     QPKEKCRIRVDDQIHVWDEGQSIVFDDTYEHEVWNDTDGFRVVLFVDFVRPIKFPYNL
                     INKFMVFAAQYIPAMQEASQNQKKWEKDFYKK"
     gene            complement(4497..6050)
                     /locus_tag="AC159U_01770"
     CDS             complement(4497..6050)
                     /locus_tag="AC159U_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR3335816.1"
                     /translation="MSNNRSGKRELWNSKTGLILAMAGNAIGLGNFLRFPVQAAENGG
                     GAFMVPYIISFFLIGIPIMWCEWAIGRYGGSKGHGTTPSIFALLWDHPAAKIIGVLGL
                     WIPLIVAIYYIYIESWTLGYSLHFLLGSSPEIPTTNTEDVNAYIKPFGDFLGTYIGAG
                     EVFLKPSTIAYTAFVITLIINFTILLKGISRGIEIFAKFALPTLFILAIILLIRVLTL
                     DTPHGNAIAGLNFLWTPDLQALKNPNVWIAAAGQMFFTLSLGFGAIITYASYVKHDHD
                     ITLSGLTSASLNETVEVVIGGSIAIPAAVAFFGVTGAIVIAQSGAFSLGFVSVPAIFA
                     SMPLGNLFGFLWFFLLFFAGLTSSVAITQPIITFFQDEFSYTRTKSIVVSYIIIVVSV
                     IMVIFVDKTIDEWDFWAGTIGVVLLGFFELIIFIWIFGGEKAWEEVNRHGLIRAPKIF
                     YYIMRYITPLFLLLLIAFWGYDFLPKILEQSSWNIWLSRAYIIGLFVFLTFLVFRSES
                     KKEAKPIGN"
     gene            6240..6749
                     /locus_tag="AC159U_01775"
     CDS             6240..6749
                     /locus_tag="AC159U_01775"
                     /inference="COORDINATES: protein motif:HMM:NF044994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3335817.1"
                     /translation="MPRQKKADTEKTKKTTRAAATKKTTAKAAKKTTKTSAAKDLSAT
                     PKPTKKAKASDWKDEIKLMLEQMKKELLQDVGKSIKDESDYLKNDVGDFYDHASNDRD
                     RELALMFTDRERTKLIQIDESLKRIEEGVYGECDNCMDEIGEDRLRVMPFAKLCLSCK
                     IELERHGEL"
     gene            complement(6746..7786)
                     /gene="mtnA"
                     /locus_tag="AC159U_01780"
     CDS             complement(6746..7786)
                     /gene="mtnA"
                     /locus_tag="AC159U_01780"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR3335818.1"
                     /translation="MSKFSTIEWKDGSVVMIDQRKLPTEETYIKCTTSAQVAKAIKEM
                     VIRGAPAIGVAAAMGVALGAKKIRAKNKDIFFSKLKSLSDEIASTRPTAVNLFWAVKR
                     MNDLANSLKNEEIENIVTRLIQEAELIRKEDIEICKDIGKMGAKLLKKGNNVITHCNA
                     GALATAGYGTALGVLRAASEQGKNIHVYSTETRPFLQGSRLTAWELSKDNIPVTIITD
                     SMAGHVMRSKNIDAVVVGADRIAANGDVANKIGTFSLSILAKHHKIPFYVAAPLSTID
                     VNCASGDKIPIEQRDPAEISTIKGKRIAADTDIYNPAFDITPNKNIDAIITEKGVITR
                     PYIKSISKLFNN"
     gene            7949..8311
                     /gene="dksA"
                     /locus_tag="AC159U_01785"
     CDS             7949..8311
                     /gene="dksA"
                     /locus_tag="AC159U_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR3335819.1"
                     /translation="MKATDLKMYKDLLQQRMDMLMGTAGETLDDLSQGDDPFPDPLDR
                     AMSESNRSIELRKRDRERKLIQKIKQAIARTEDGTYGVCDDCGEDISANRLKARPETT
                     LCINCKEEQEKVEKQFGL"
     gene            complement(8317..8781)
                     /locus_tag="AC159U_01790"
     CDS             complement(8317..8781)
                     /locus_tag="AC159U_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3335820.1"
                     /translation="MKKSIKYVVLTLLFALPILSCGNADNNDIKKVLSDRQTAFETKD
                     LQLYLSCISPDYSQKAEDSVIGIEELSKNFQTNTTVFDTLKITRKDISIYMKGQGIAE
                     VYQKSLFNLKIESQSSKFKTVEKMLFEKKGGNWKIVKESELDLFRGFVFGSK"
     gene            complement(8784..>9533)
                     /locus_tag="AC159U_01795"
     CDS             complement(8784..>9533)
                     /locus_tag="AC159U_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3335821.1"
                     /translation="LITLTLIIGCGSKKPIEGNNAKAMYDKALEELSKDKGGFFGLFN
                     ATDYGTIEETLKEIQIRYTYSPYATLAELRTADTYFKRGEYEQAAIEYEEFIKRHPSH
                     NGTEYATFFLALCNFKSIRGHDRDPSGAREAVKWLNEYIEQYPDSPLVQQAHNNIRET
                     IDVLAEREIYIGDYYYNKNNFKASSERYKTVIDQYPQTTHYAKALYLLGKSYSDMNEN
                     SLALSALNRVINEFPNSKYNKDALELASRIK"
BASE COUNT         3107 a         1683 c         1863 g         2880 t
ORIGIN      
        1 gcagatatga aagccttttc cgaatcacat agatcgcagg gaaatactat ttcatttgta
       61 cctacaatgg gtgcactgca tgaaggtcat ctgcagctag taaaaaatgc gcgcaatctt
      121 tcagacatat tagtagtaag catattcgta aaccctacac agtttggtcc caatgaagat
      181 tttgataaat accccagaga tctggacaaa gataaaaacc ttctggaaga gctgggagta
      241 gatgccatat ttttcccgag tgcagacaat atgtaccccg gcggttttca aaccactgta
      301 gaagtgcagg aaattcaaaa gccactttgc ggaaagttca gacccggtca tttcgggggc
      361 gtggttacag ttgttctaaa actattcaat attgtaaaac cccattatgc agtttttggc
      421 gaaaaggatt atcagcagct atgtgtaata agacaaatgg tttctgacct gaatgtggat
      481 gttgaggttc tgtcccaccc aattgtaagg gaggagagtg gtcttgcaat gagttctagg
      541 aatgaatatc taaaaagtga caaaagacaa caagcagcat caatatctga tgctctgttt
      601 aatgtaaaaa agcagtttga cagcggagtt aatgataaga aaaatttact ggacactgct
      661 tattcaatct tgaatgaagc ggatatacgc gaaattgaat atttagagat agtagacgga
      721 caaaatttaa gtgaaaaaaa agcggtgagc tctggagatg tggtagcagt agcagcaagg
      781 ctggatggaa ccagactcat agacaatata aggctttgat aaggaggtaa gataatgcac
      841 cgcacattat taaaatctaa gattcacagg gcaacggtta cagatgctaa tgttgactat
      901 gaaggcagcg ttaccattga ccgcgagctt atggatcttg cagacctgca ggaatatgaa
      961 caggtgcata tctataacat tacaaatggt aacaggcttt ccacttatgt tatagaaggg
     1021 cagaggaact caggacatat atgcataaat ggtgcagcag ctcatctggc aaaaacaggc
     1081 gatctggtga taattgcaaa ctatgcagat tactcagaaa atgaggctag tctccacaaa
     1141 cctaagctga tttatgtaaa tgatgataat tctgttaaac atatatcaga tcatgttgca
     1201 aagctgaagg tagcaggctc gtaataaata attttgaaat tacctgacat tatacaaatt
     1261 gccatagttt gagaaagttt ctgtggtgat tgttagaaat atttatgatt ttaatgtaaa
     1321 attcaattta aaattttgat gcatctttta aaagatacaa aacatcataa tattgacatc
     1381 taagaagaaa gaggttataa atctaagttt attaaaggag atggtaggat gaaggtgtat
     1441 ttttattcaa aaatacttat aaaacctacc tatgttgcaa ctcagcaact gtaataactg
     1501 atttggagaa tttggagaaa tactaatgaa agcaattata cttgcagcag gtaggggcaa
     1561 aaggctatat ccttatacaa aatatattcc taagtgtttg ttggatgtag gcggggttac
     1621 catccttgaa catcaaataa acagcattag agcttgcggg attgacgaag tagtaatagt
     1681 aattggtttt ggatttgaaa aagtagaaga ttttctgaga aactacgatg gactgggttt
     1741 aaagattaag acactttata atccattcta tcaaacaacc aacagcctga tttcactctg
     1801 gatagcacgt ggtgaaatgg atcaggatat agtagtgatg aatggtgatg atgtgtttga
     1861 acttgaagtg cttgattcgg cgctcagtat gctcgaagat aagatttgcc ttcctgcaaa
     1921 gaaaagagac gagtatgaag atgaggatat gaagattgta gtaaaggaag gaaacatcgt
     1981 agaagttggc aagtcacttg agagttcacc aagcgcagaa tcagtcggcg taagggtttt
     2041 tagagataca ggcgtagagc ttttaaaaag atcgattgaa gaggagatga ggtcagagaa
     2101 tcctgagaaa aaatggtata tatctgcaat acacaggctc attgaaaaag gatacaaagt
     2161 taaatccttt gatgtagaag atatgttctg gatggatgtt gactatcccg gagacctctt
     2221 taaagcaaga attaatgcaa gcaagtttat agatagcaag ttccagacgg atgtgccata
     2281 cgaacaaaga ttgcttaggg ttgttgatag caacagataa tactacaaat gtatcatgca
     2341 atcattgttg caagcggcac cggcattgac ggggcagatt tatccgaatt tcccaccaaa
     2401 gaactcgtcg gagttccgca gctaaacaga ctaataattg tggctcagag ggcaggtata
     2461 agtaaattta ccgtcctaac ccaaactgac aattatcatg cactggataa tattgaaact
     2521 gataagagaa tatcttctag tgtcaactgg cataaaaagg gagaaaagat tagtttggat
     2581 gatgagcact gccttgtttt acaatccaat ctcgttatta ctcctgaaag cctaaaagat
     2641 tttattaatc aaaaaattgc taaaaatgat tttttgattc ttgtagatga gttagataat
     2701 ccttctctta cactaaacaa tggtaaaatt gaaggcagct ttaaaaacag tgggcaggca
     2761 ataggtgctt tcatatctac tggtaaggag cttaaaaaga taattaactc ttctgactct
     2821 ataggcagtt tcgtatctaa taaactgagc tcttcagcaa aaatcaaata tcagcaaata
     2881 accagtggct actggtatca tttaaatgat actgatcaat cttttaatga ggcaaaagaa
     2941 atacttttct caaatgttgg taaaaccgct actggctggg tatcgagaaa aataaacagc
     3001 agattttcac ttcctacaag ccggcagctt gttaagactt ctcttactcc caatatgata
     3061 agcgtgttgt taaatgtaat aggtatgatg tcaggagtat tccttgctct cggtcatccg
     3121 gtatggggct gttttttctt gtatgttgca acagttttag acagatgtga tggtgaagtt
     3181 gcaagggtaa agctcatgga aaccaaaacc gggcagtggg tggatacaat atctgatcaa
     3241 tttactgtgc tctccttttt tattggtctt acaataggat attacagggt gtcggacagc
     3301 aatcttgcta tattctttgg cctttttaac ctgtttgtat ttttcttctt tctaatctgg
     3361 tcttttcatt atctttccaa atatacaaac tcgggaagtc ttgtagaata tttcacaata
     3421 gataaatacg aggataatcc ctcttttata agaaggttta ttttatttct aaggccgctg
     3481 tcaagaagaa acgcttatgc tatgggattt atgattgttt ctattattgg tggatacacc
     3541 tggatgttcg gacttcttac atttgcaatg ttgctgtttt ttattcatct tatagaagat
     3601 tatttaaata ttaaaaatta ttcaaatcaa aagggctgat ctaatatcat gagtaatgac
     3661 aacacctcaa aaggttttaa tccacccaaa aagtacgcag gagggctttc atcattcatt
     3721 aactgggctg agggaattaa tgaaagatat tccataaacg gcaacccgcc agtatatgat
     3781 aattctagct ttccgtgggt aaaagaagtt gaagatgagt ggcttagtat tagaagtgag
     3841 cttgaggatg taatgaagca cagatcagaa cttccaaatt tccagaatat tatgcctggg
     3901 gtggatgcga taactactga taataactgg aagacttatt ttcttgcagg atatggtatt
     3961 gaaagtaagg aaaactgtaa aaggtgtccg gaaacagcaa ggattcttaa aaaaatccct
     4021 ggtatgaaga cagcattttt ttcaatacta tcgcccaata aacatatccc ggctcataaa
     4081 ggccctttca acggagtgtt aagatatcat ctggggctta tagttccaca gccaaaggaa
     4141 aaatgcagga taagagtaga cgaccagatt catgtatggg atgaagggca aagtatagta
     4201 tttgatgata cctatgaaca tgaagtctgg aacgatactg atggcttcag agtagtgctc
     4261 tttgttgatt ttgtaagacc aataaagttt ccatataact tgattaataa atttatggtc
     4321 tttgcagccc agtatattcc ggcaatgcag gaagcaagcc aaaaccagaa gaagtgggaa
     4381 aaagatttct ataaaaaata gaactttatt tctttattgc agtcataagc ttataaatgc
     4441 agtaggtcaa cagagataaa ataaatcccc aggaaccagt cataaacata agagcactaa
     4501 tttccaattg gcttagcctc cttttttgat tctgatctga acacaagaaa cgttaaaaat
     4561 acaaaaagtc ctataatata agctcttgaa agccagatgt tccatgagct ttgctcaagt
     4621 atctttggca gaaaatcata gccccaaaat gcaatcaaca gaagtaaaaa tagaggggtt
     4681 atatatctca ttatgtaata aaatatcttt ggagctctta taagtccgtg cctgtttact
     4741 tcctcccatg ctttttcccc tccaaaaatc caaataaata ttataagctc aaaaaacccc
     4801 aaaaggacaa ctcctattgt tcctgcccaa aaatcccatt catcaattgt tttatctaca
     4861 aaaataacca ttattacgct tactacaatt ataatatatg aaactacaat cgacttggtt
     4921 cgtgtataag aaaattcatc ctgaaagaat gttatgattg gttgggtaat agcaacagac
     4981 gatgtaagac ctgcaaagaa aagaagaaaa aaccataaaa acccaaataa gtttccaaga
     5041 ggcattgatg cgaatattgc aggcacacta acaaaaccaa ggctgaatgc tccggactgg
     5101 gctattacaa tagctcctgt tactccaaaa aatgcaacag ccgctggaat agcaattgaa
     5161 cctcctatta caacctcaac tgtttcattt aatgatgccg aagtaagacc actcaaggtt
     5221 atgtcgtggt catgcttaac atatgacgca taggttatta ttgctccaaa acccaggcta
     5281 agagtaaaaa acatctgtcc tgctgctgca atccatacgt ttggattttt aagagcctgc
     5341 aggtcggggg tccacaggaa atttagcccg gcaatggcat ttccatgcgg agtatccagg
     5401 gttaatactc taataagaag aattattgca agtataaata aagtgggaag cgcgaatttt
     5461 gcaaaaattt ctatacccct gctaatacct tttaaaagaa ttgtgaaatt aataataagg
     5521 gtaattacaa atgccgtata agctattgtc gaaggcttta aaaatacttc accagctccg
     5581 atatatgtac ctaagaagtc accaaatggt ttaatataag catttacatc ttcagtattg
     5641 gtagtaggaa tttcagggct tgagccaaga agaaaatgaa gagaatatcc aagggtccag
     5701 gattcaatat aaatataata tattgctact ataaggggaa tccaaagccc gagaactcct
     5761 attatttttg cagccggatg atcccataag agggcaaata tcgaaggagt agtaccatgg
     5821 cccttggagc caccgtatct gcctattgcc cattcgcacc acataatagg gataccgatg
     5881 agaaaaaatg agattatata gggcaccata aacgccccac cgccgttttc agctgcctgt
     5941 acaggaaagc gaaggaaatt gccaagtcct atagcgtttc cagccatagc cagaataagg
     6001 cctgttttag agttccatag ctctcgtttg cctgaacgat tgttgctcat ttagttaatt
     6061 ctatactaat atatcttaaa ttcggtataa aataagcttt actcataata tcacatttgt
     6121 aataaatttg aaaaaacatg attttttaca tcagagtttg tatgattatt acttgacaaa
     6181 aatgattgcc tgcttatctt aataaacctt aaaattttca ggtgatgaca gagactaata
     6241 tgccaagaca gaaaaaagct gatacagaaa aaacaaaaaa aacaacacgt gcggcagcca
     6301 ctaagaaaac taccgctaaa gcagccaaaa aaactaccaa gacatctgct gctaaagatt
     6361 tatctgcaac tcctaaaccc acgaaaaaag caaaagcttc tgactggaaa gatgaaatta
     6421 aactaatgct tgagcagatg aaaaaagagc tgttgcagga cgtcggaaag tcaataaaag
     6481 atgaatcgga ctatctaaaa aatgacgtgg gtgattttta cgatcatgca tccaatgaca
     6541 gagacagaga acttgctctt atgtttacag atagagagcg taccaagctt attcaaatcg
     6601 atgaatccct aaaacgcata gaagaaggtg tttatggaga gtgcgataac tgtatggatg
     6661 aaataggcga agacaggctc agagtgatgc cctttgcaaa actttgccta tcgtgcaaaa
     6721 ttgagcttga aagacacggt gagctctagt tattaaaaag ctttgatatt gatttaatat
     6781 aaggacgagt aatcacacct ttttcagtaa taatcgcatc aatatttttg tttggtgtaa
     6841 tatcaaatgc agggttatag atatctgtat ctgctgcaat cctcttaccc ttaatagtgc
     6901 ttatttcagc aggatctctt tgttcaattg gtattttgtc tcccgaagca cagttaacat
     6961 cgattgtaga tagtggagct gctacataaa atggaatttt atgatgctta gcaagtattg
     7021 atagtgaaaa agtgcctatt ttattagcta cgtctccgtt tgcagctatt ctatcagccc
     7081 ctactactac tgcatcgatg tttttacttc tcataacatg gcctgccata ctgtcggtaa
     7141 taatagtcac agggatattg tctttactaa gctcccaggc ggtaagtctg gagccctgta
     7201 gaaagggcct tgtttcagtg gagtaaacat gaatgttctt gccctgctca ctagctgccc
     7261 tcaaaactcc tagcgccgta ccataacctg ccgttgcaag agcgccggca ttacagtggg
     7321 taataacgtt gtttcccttt tttagcagtt ttgcacccat ttttcctata tctttacaaa
     7381 tctcaatgtc ttctttgcgt ataagttctg cttcttggat aagtcttgta acaatatttt
     7441 caatttcctc attttttaga gaatttgcaa gatcattcat tctctttaca gcccaaaaga
     7501 gatttacagc agtagggcgg gtagatgcta tttcatctga taaggatttt agcttactaa
     7561 aaaatatatc cttatttttc gctcttattt tttttgcacc aagcgcaaca cccattgctg
     7621 cagcaactcc tattgcagga gcgcccctta ttaccatctc tttaatggct tttgcgacct
     7681 gtgcagaggt agtgcattta atatacgttt cttcagtagg aagtttgcgc tgatctatca
     7741 taacaacgct tccatctttc cactcaattg tactaaattt gctcaactat actctcctgt
     7801 ggtctttatt attaatgatt agatgaatcc caactttaga aaataattga tgtctttata
     7861 tatacaatat ttcacattgt caacaattga ctatcaataa taaattgata gactcatgta
     7921 ataaattaat taatttattg aggatttgat gaaagcaact gatctaaaaa tgtataaaga
     7981 cctcctgcag cagcgtatgg atatgcttat gggcactgca ggtgaaaccc tggatgacct
     8041 gagccagggc gatgatccat ttccggaccc tctggacaga gcaatgtctg aatcaaatag
     8101 aagcatagag cttagaaaga gagaccgcga gagaaaactc atacagaaaa tcaaacaggc
     8161 aatagccagg actgaggacg gtacttatgg ggtgtgcgat gactgtggag aagatatatc
     8221 ggcaaacaga ttaaaagccc gccctgagac tactctttgt ataaactgca aggaagaaca
     8281 ggaaaaagtg gaaaagcagt ttggattata atttttctat ttagaaccaa aaacaaatcc
     8341 cctaaacaga tcaagttctg attcttttac aatcttccag ttacctccct ttttttcaaa
     8401 aagcattttc tcaaccgttt tgaatttgct ggattgtgat tctatcttta ggttgaaaag
     8461 cgatttttga tacacttcgg caataccctg ccctttcata tatatgctaa tgtcttttcg
     8521 tgtaattttt agtgtatcaa aaactgttgt gtttgtttgg aagtttttgc ttagttcttc
     8581 aatacctatg acagaatctt ctgctttttg gctataatct ggagaaatgc agctaagata
     8641 gagttgtaaa tcttttgtct caaatgcagt ctgcctgtca ctgagcactt ttttgatatc
     8701 attgttatca gcatttccgc acgataatat aggaagtgca aataacagcg tcagtactac
     8761 atatttgatg ctctttttca tccctacttt attctggatg caagctctaa agcatcttta
     8821 ttatattttg aattgggaaa ttcatttatt accctattga gcgcagatag agctaggcta
     8881 ttttcattca tatcagagta tgactttcct aaaaggtata gtgcctttgc atagtgtgtt
     8941 gtctggggat attgatctat aaccgttttg tagcgttctg aagatgcttt aaagttattt
     9001 ttattgtaat aatagtctcc aatatatatt tctctttcag caagtacatc tattgtttct
     9061 cttatattat tatgtgcttg ttggactagt ggagaatcgg ggtattgctc tatatattcg
     9121 tttaaccatt taacagcttc ccttgcacca cttggatctc tgtcgtgtcc tctaatagat
     9181 ttaaagttgc aaagtgccag aaaaaaagtt gcatattcgg tgccgttgtg agaagggtgt
     9241 cttttaatga attcttcgta ctctatagcg gcctgctcat actctccccg cttgaaatat
     9301 gtatctgctg ttctaagctc ggcaagcgtt gcgtatggac tgtatgtata tcttatttgt
     9361 atttctttta gagtttcttc aattgttcca tagtccgtag cgttaaaaag gccaaaaaag
     9421 ccgcctttat ccttgctcag ctcttccagg gctttgtcat acattgcctt tgcgttattt
     9481 ccttctattg gttttttaga accacaccca ataattaatg ttaaggtaat taa
//
LOCUS       DBNGSV010000049         5493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1809143, whole genome shotgun sequence.
ACCESSION   DBNGSV010000049 DBNGSV010000000
VERSION     DBNGSV010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1809143"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1380)
                     /locus_tag="AC159U_01800"
     CDS             complement(181..1380)
                     /locus_tag="AC159U_01800"
                     /inference="COORDINATES: protein motif:HMM:NF022379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HIR3335822.1"
                     /translation="MSVLRVPSGKHSPLGASGAKRWMNCPGSINLINKLGDHAGRSGK
                     EAAFGTAAHTIAAMCLQENKEPWEFGGTHIEVDGWKFVVDQDMLDSVTSYVDFVWKKG
                     TEYDKYGPGIFVEKGMSSELHEDAYGTSDCVIYVPGDRIIVIDFKNGINIPVEPDDPQ
                     LKYYGYLAYENRPSYMKDKGEPTIIELWIAQPRLMHPQGPFRSTLYSVNDVVEWFGNS
                     VLTAMENTKDPKASLKMGEWCQFCPARDHCPVLKKEALEYPVDADPEELDEATIGLLL
                     DKGEILKKYLEKLQIVAFHRAQQGKKIPGRKLVRKISRRQWKVGAEEYFVDEYGEDAY
                     APVTLLSPAQMEKFLDGELNAKEIIEEWSMKPDSGLTLAAESDKRIEVSPLLEYDPML
                     LDDAIDV"
     gene            complement(1380..1796)
                     /locus_tag="AC159U_01805"
     CDS             complement(1380..1796)
                     /locus_tag="AC159U_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335823.1"
                     /translation="MVQKTEPTDEGAQDQETAESESDSEQDETPKRRGRPPGRKNKSQ
                     SDESETEETLNLDPPEEDNVVSLNPNGTDTEEPEITLAQIKTLMNKLTGDGTLIPKVR
                     SLLDQFGVSSTTELDEAKYPEFFKMLSGIKAEPLEL"
     gene            complement(2057..2272)
                     /locus_tag="AC159U_01810"
     CDS             complement(2057..2272)
                     /locus_tag="AC159U_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335824.1"
                     /translation="METTACDILKPILFILSHIAVAVVFFIVGAMCCSGKPDPTILPT
                     KKSLTVDALCWPDGDHTPPEYYKKDGS"
     gene            complement(2253..2513)
                     /locus_tag="AC159U_01815"
     CDS             complement(2253..2513)
                     /locus_tag="AC159U_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335825.1"
                     /translation="MMAQEKVVFALNERGNLVPFMIKIETRDDTDYILEKEMLFLTDV
                     EFPCDGAHGFATGLTVGVYWKTLKDAEAKAPMPGERNGNNGV"
     gene            complement(2510..4135)
                     /locus_tag="AC159U_01820"
     CDS             complement(2510..4135)
                     /locus_tag="AC159U_01820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3335826.1"
                     /translation="MELEIRREAGEKLTYNEKNNKFVYFAHSPKKADEIGLIRSRSAR
                     GPGGTQVWFTDEPFAALALWDVADDTARNKLSRLKSEFDKSYSTESPGDFPCPEGKQY
                     MPYQRAGIQFGVDRGNCLIGDEPGLGKTVEAVGIANTLDARRILVVCPASIRLNWIRE
                     INSWSTLGNVRSRAILSGRDRLEEGFNYTVVSYELAKSERFHEYFCNDYFDLLILDEA
                     HYLKTNTSQRTRSVFGGGRGRFQNFRLADAAERIIALTGTPLPNRPRECYTLARGLDW
                     ESINWLSYEKFCNRYNPSASFWSGRRWEAVGRLPELRARLRCNFMIRRHKSDVLLDLP
                     DKRYEMSYIEPNGEIREILKREKLIDFDPDHIFNPKIEMNGEAIATLRREMGVAKIPR
                     VVEHVKYILDILEIPKIVLFAHHRDVLNTLMNELSRYHPVLRMGGMGTSRQQAAIDMF
                     VGNPDCRIFMGQLDTMEGADGLQHMTQYVIFAEPAWTPGRNEQCVDRLHRFGQHGNVI
                     AQFLIAEGSMDEKVLHAVLGKAQVIHRTLDGDL"
     gene            complement(4358..4783)
                     /locus_tag="AC159U_01825"
     CDS             complement(4358..4783)
                     /locus_tag="AC159U_01825"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR3335827.1"
                     /translation="MRKVLDWDAYYMEMAAAAAERSKDPSTQVGAYIVDRFGTPISAG
                     YNGFLPGFPDTEENWQRPQKYDYVVHAEANAVGHAARRVLEGSTAYVTAMPCSGCIKS
                     LISAGISNIVHGPPVHGWDKEHELAMKLAGLTGTEMREI"
     gene            complement(4788..5165)
                     /locus_tag="AC159U_01830"
     CDS             complement(4788..5165)
                     /locus_tag="AC159U_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335828.1"
                     /translation="MSGPSTELVMRIARVLRDTVHHTNQAERARQIISAFQPGDEVRE
                     GLQVIDLVDYFRQQNAIMRKTRADARQKGARQAAGAMQETCALEAGNWIQGFASQETC
                     QALTNYIRTLDTDKILETKSNRG"
     gene            complement(5162..>5493)
                     /locus_tag="AC159U_01835"
     CDS             complement(5162..>5493)
                     /locus_tag="AC159U_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335829.1"
                     /translation="VAVHHGLCIGADADFHNIAKSLGIPIIGHPPIKKNLMADLPRED
                     FKHIYSPRPYLVRNRAIVRYSDAIYATPKEFKETVRSGTWHTIRHARQQNLNVTVIYP
                     DGNVEYL"
BASE COUNT         1505 a         1353 c          982 g         1653 t
ORIGIN      
        1 ttaaactttg ttagatcaga aggctcaatc aatagctcag caccatagcg ccgagtctta
       61 ggatcattca taaccggttt aggttcgata accgacggat atgatagcct tgcaaggggg
      121 gttaaaagtc tacgttggcc tccatctgcc atttcatttt cctttcaagt ttttaatcag
      181 ttaaacatca atagcatcat cgagtaacat tggatcatat tccaataacg gtgatacttc
      241 tattctttta tcagattccg ccgctaatgt caaccctgaa tctggtttca tcgaccattc
      301 ttcaatgatt tcttttgcgt ttaactctcc atctaaaaac ttttccattt gtgcgggaga
      361 gagtaacgtg accggcgcat aagcatcttc cccatattca tcaacgaagt actcttctgc
      421 accaaccttc cactggcgtc gtgaaatctt tcgaacaagc ttccggccag ggatcttttt
      481 accttgttgt gccctatgaa aggcaactat ttgtaatttc tcaagatatt tctttaggat
      541 ttcaccttta tcaagcaaca atcctatggt ggcttcatcc aactcttcag gatcagcgtc
      601 aacaggatac tccaaggctt cctttttaag tacaggacag tgatctcggg caggacagaa
      661 ttgacaccac tcccccatct taaggctggc tttagggtct ttagtgttct ccatcgccgt
      721 taagacagag tttccaaacc actctacaac atcattaaca gaatataaag ttgaacgaaa
      781 cggtccttga ggatgcatta accgaggctg agcaatccat agttctataa ttgttggctc
      841 ccccttatct ttcatataag aaggacgatt ttcataggct aaatacccgt aatatttgag
      901 ttgtgggtca tctggttcaa ccggtatgtt gattccgttt ttgaagtcaa tgacgataat
      961 acgatctccg ggcacgtaga tgacacaatc ggatgtacca taggcgtctt cgtgcaactc
     1021 acttgacatt cctttttcaa caaaaattcc agggccatat ttatcatatt ctgttccctt
     1081 cttccataca aaatcaacat aggaagtaac cgagtctaac atatcttgat cgactacaaa
     1141 cttccagcca tcaacttcaa tatgagtgcc accaaattcc cacggctctt tgttttcttg
     1201 taaacacatt gccgcaatgg tgtgggcagc cgtaccaaaa gccgcctctt taccactacg
     1261 gccagcgtga tcacctagtt tattaattag attaatagaa ccgggacagt tcatccacct
     1321 cttagcacca gaagcgccaa gaggactgtg cttacctgaa ggtacgcgaa gaacagacat
     1381 tatagctcca atggctcggc tttgattcca cttagcatct taaaaaactc gggatacttg
     1441 gcttcatcaa gctctgtggt tgaggataca ccgaattgat ccagaagact acgaacttta
     1501 ggaataaggg tgccgtctcc ggtaagctta ttcataagag ttttaatttg cgccaaagta
     1561 atctctggtt cttcagtatc cgtgccgtta ggatttaatg ataccacatt atcttcttct
     1621 ggggggtcaa gattcaaagt ttcctctgtt tcagattcat cagactgcga cttattttta
     1681 cggccaggtg gtctaccacg tcgttttggg gtttcgtcct gttcactatc agattcagat
     1741 tcggcggtct cttgatcttg tgccccttca tcagttggtt ctgtcttttg aaccacttta
     1801 acttgagcac cttttccaga acgaccctga aacgcttgag tgacagcatc acgtacctct
     1861 tgcataatat gttcacggac ttccctagga tcatccgttt catccaactc aaccttcatt
     1921 tcaaattcgc attcagcggg ctcaaattgt ttaacctgaa ccttccgcga aaaccttgca
     1981 ctcatttcat atacacgcat cgaacaattc tccaattttt ttcaggaata tatcatacac
     2041 tcttttgccg ctaatgtcaa ctcccgtcct tcttataata ttccggggga gtgtgatccc
     2101 catcaggcca gcaaagagca tcaacagtaa gtgatttctt tgttggtaag atagtaggat
     2161 caggtttacc gctgcaacac atagctccaa caataaaaaa tacaacagct acggctatat
     2221 ggctgagaat aaataaaatg ggctttaaga tatcacacgc cgttgtttcc atttctttct
     2281 cctggcatgg gagcctttgc ttcagcatcc ttaagagttt tccagtagac acctacggtc
     2341 aaaccagtag caaacccatg agccccgtca caaggaaact caacatcagt aagaaataac
     2401 atttcctttt ctaaaatata gtctgtatcg tctcttgttt caatcttaat catgaaagga
     2461 accaaattac ctctttcatt aagagcaaaa actacttttt cttgtgccat cataaatctc
     2521 catctaaagt gcggtgaata acttgtgctt tacccagaac agcatgtagt accttctcat
     2581 ccatagaccc ttctgcaatt agaaattgag ctatcacgtt tccatgttgc ccaaatctat
     2641 gaagcctgtc cacacactgc tcattacgtc ctggcgtcca agctggttct gcaaatatta
     2701 catattgtgt catatgttgc aaaccatcag ccccttccat agtatccagt tgtcccataa
     2761 aaattctaca atcaggattg ccaacaaaca tatcaatagc ggcttgttga cggctggttc
     2821 ccataccgcc catacgtaag acagggtgat accggcttag ctcattcata agagtattaa
     2881 gtacgtctct atgatgagca aacaaaacta ttttaggtat ttctaatatg tctaaaatat
     2941 attttacatg ttctactact cttggtatct tagccacacc catttcacgt cttagtgtag
     3001 ctatggcctc tccgttcatc tcaatcttag ggttaaaaat atggtctgga tcaaaatcta
     3061 ttagtttctc tctttttaaa atttccctaa tctcgccatt aggttctata taagacattt
     3121 catatctttt atcaggaaga tctaataaca cgtctgattt atgccttcgg atcataaaat
     3181 tacagcgcaa cctagcacgt aattccggca gtctacctac agcttcccat ctacgaccag
     3241 accaaaaaga agcagaaggg ttatacctgt tacaaaattt ttcataactc aaccagttta
     3301 tagattccca atctagtccc ctagccaacg tataacattc acggggacgg tttggcaaag
     3361 gggttcctgt taatgcaata attctttccg ctgcatccgc caatctaaaa ttttggaatc
     3421 ttcctcgacc acccccaaaa acagatctag ttctttggct tgtgtttgtt ttaagatagt
     3481 gtgcctcgtc taaaataaga agatcaaaat aatcattaca aaaatattca tgaaatcttt
     3541 cactcttagc taattcgtaa gagacaaccg tgtaattaaa tccttcctca agtctatctc
     3601 ttccagagag gattgctctt gatctaacgt ttccaagagt agaccaggag ttaatctctc
     3661 taatccagtt gagccgaata gacgctggac agacaaccaa aattcgtctg gcgtccaacg
     3721 tgttggcgat tcccacagct tcgaccgttt ttccgagccc aggctcatcc ccaatgagac
     3781 aattccctcg gtccactcca aactgtattc cagcccgctg atagggcatg tactgcttgc
     3841 cttcaggaca ggggaaatct ccaggggact cggtggagta gcttttatcg aactctgact
     3901 tgagcctact gagcttgttc cttgctgtgt catctgctac atcccataat gctagagccg
     3961 caaaaggctc atctgtaaac cacacttgtg tgccacccgg acccctagca gatctagatc
     4021 taatcaaacc aatttcatct gcttttttag ggctatgagc aaagtataca aacttattat
     4081 ttttttcatt atatgttaac ttttctccag cctctcgtct tatttccagt tccaaaactc
     4141 tttacctatg ttccaaaaat gtaatacggt ggagcttagg cacataaaca acacgcccat
     4201 taatccgttc tccaccgcta ccaacccaag acacagattc gctaataggc caaccacggc
     4261 tgctagcgtt actaatcttc tcccataaaa gaggatagat attaaaatcc caatcgagcc
     4321 catccattct agtgctatcc ataactcgtc cataatatta tatttctctc atttctgtac
     4381 cggttagtcc agccaacttc atggctaatt catgctcttt atcccatcca tgtacgggcg
     4441 gaccatgcac tatattagat atgccggcag aaatcaagct ttttatacaa cccgagcatg
     4501 gcatagcggt tacataagct gtggatcctt ccaacacacg cctagcagca tgacctaccg
     4561 cgttagcttc cgcatgaact acataatcat atttttgtgg gcgctgccaa ttctcttctg
     4621 tgtcgggaaa cccaggaagg aaaccattat agcctgcact aataggagta ccaaacctat
     4681 caacaatata ggctccaacc tgtgtagaag ggtctttgga tcgttcagcc gcagcagcag
     4741 ccatctccat ataatatgca tcccaatcta aaactttcct catcgtatta acccctattg
     4801 ctttttgttt ctaggatttt atcagtgtca agggtgcgga tatagttggt taaggcttgg
     4861 caagtttctt gactcgcaaa accctgtatc cagttacccg cctcaagcgc acatgtttct
     4921 tgcatcgccc cggcggcttg gcgggcaccc ttctgtcgag catcggcacg agtcttacgc
     4981 atgatggcat tttgctgccg aaagtaatcc actaaatcaa ttacctgtaa cccctctcgt
     5041 acctcatcgc cgggctggaa agcagagata atctgtcttg cgcgctctgc ttgattcgtg
     5101 tggtgtaccg tgtctcgaag gactctagca atgcgcatca ctaattctgt agacggcccg
     5161 ctcataaata ctccacattt ccatcaggat aaatcacagt aacatttagg ttttgttgtc
     5221 ttgcgtgtct aatagtgtgc catgtaccag aacgcaccgt ttctttaaat tctttcggtg
     5281 ttgcataaat ggcgtcacta taccttacta ttgctctatt gcgaaccaaa tatggtctag
     5341 gagaataaat gtgcttaaaa tcctctctag gtaaatcggc cataagattt ttcttgatag
     5401 gtggatgccc tataattggt atgcctaaag atttagctat attatgaaaa tctgcatctg
     5461 cgcctataca tagaccatga tgcacagcta cga
//
LOCUS       DBNGSV010000050         2335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_771384, whole genome shotgun sequence.
ACCESSION   DBNGSV010000050 DBNGSV010000000
VERSION     DBNGSV010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_771384"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="AC159U_01840"
     CDS             <1..451
                     /locus_tag="AC159U_01840"
                     /inference="COORDINATES: protein motif:HMM:NF036468.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIR3335830.1"
                     /translation="PAALIEVKVDFSQMTIYIKKRVYGTGIGLNDLVSRIKSSIVSPM
                     AQIIADSADPLLIDHLKSVHRLNIFKAVKGPNSVIFGISKMNDFSLIIAEGSDEIETE
                     FKNYRFKEDANGDPLEEPIDDFNHAIDAIRYAAMHKISKKNNKLIYG"
     gene            448..696
                     /locus_tag="AC159U_01845"
     CDS             448..696
                     /locus_tag="AC159U_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335831.1"
                     /translation="MIYKDFDKELKTLEEMFNEHTSDKKINGYHRAVLHGFAHLRRKL
                     SFLIKGQELETKTGKEYFLAKDGVTPETPIINDKKRKK"
     gene            781..1599
                     /locus_tag="AC159U_01850"
     CDS             781..1599
                     /locus_tag="AC159U_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335832.1"
                     /translation="MLLLTGVIVAGYQLPGFGWASLSLQGSFINTTKTIIMINLTIKG
                     DKYQIPESIKEIKLSKYIDFLYFVDANEPKVLKELRAITNSEAYRERMEKMTKGEEKQ
                     FLQYIHKEVAFWGDIPYLLIRKCDRRDVELVWSILQNNLGVREDESFNCFELQGDIYY
                     MPKRMMQDSTFEDYTEACSYEEALSEVHNGVYQALFYVAAIVSRKEKSSGERESFDDY
                     NQEERAEFFKQHLSAFDAFQIGFFLRRLSEQSLTDFQIFTAASTLSRLKLVTNN"
     gene            1763..>2335
                     /locus_tag="AC159U_01855"
     CDS             1763..>2335
                     /locus_tag="AC159U_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335833.1"
                     /translation="MLLIQVSNILNDFVQADPDINFFHCGLPNDVNVNIPNNYDPNSR
                     IGNQFPYVLLIPPIVQGRVEANGEQGIFETFPVDILISDLYGQTNSGIYGTNTYALLL
                     SKLQVIAQRIVKAFQSYAEYASPQFNVGDYTLTFDSLRHTQNTRSLRLQFDLIFALSC
                     SDYTIDLSTFATDAGDTVRYDKEDPINTTPV"
BASE COUNT          745 a          409 c          526 g          655 t
ORIGIN      
        1 tcctgctgca ttgatagagg taaaagtcga tttctctcag atgacaatct atatcaaaaa
       61 aagagtttat ggaacaggta tcggattaaa tgatttagtt tcgaggataa aatccagtat
      121 cgtgagcccg atggctcaaa tcattgcaga tagtgctgat ccattactca tagaccattt
      181 aaaaagtgta catcgattaa atatttttaa agctgtcaag ggccctaact cggttatatt
      241 tgggatatca aaaatgaatg atttttcttt aataatagcg gagggctccg atgagattga
      301 gacagagttt aaaaactaca gatttaaaga ggatgccaat ggagatccac tggaggagcc
      361 tatcgatgat tttaatcacg ctatagatgc gataagatat gcagcgatgc acaaaataag
      421 taagaaaaac aataaactga tttacggatg atttacaaag attttgataa agagctgaag
      481 acactggagg agatgtttaa tgagcatacc tctgacaaaa aaataaacgg ctaccacaga
      541 gctgtattgc atggttttgc tcatctccgg agaaagctct cttttctgat taaaggacag
      601 gagctggaaa caaaaaccgg aaaagagtat tttcttgcaa aggatggagt cactccggag
      661 actccgatta taaacgataa aaaacgaaaa aaatgaactg ggttaaaatc ggtatttaca
      721 tcactgtagc ggtgggagtt atgactatcg tgctactttt aggctgtttt attgcctggg
      781 atgctcctgc tcactggagt tatagtggca gggtatcagc tgcctggatt tgggtgggca
      841 tcgctctcat tgcagggctc ttttataaat acgacgaaaa cgattataat gataaatctc
      901 acaattaaag gagataaata tcagattccg gagagcataa aagagataaa gctatcaaag
      961 tacatcgatt ttttgtattt tgtggatgca aacgagccaa aggtattaaa ggagctcaga
     1021 gccatcacta actcggaggc atatcgggaa cgcatggaga aaatgacaaa aggagaggaa
     1081 aaacagtttc tgcaatatat ccacaaagag gtggcatttt ggggagatat tccctatctg
     1141 cttatccgaa aatgtgaccg gagagatgtc gagctggtat ggtccatcct gcagaataat
     1201 ctcggagtga gagaggatga gagttttaac tgctttgaac tacagggaga tatttactat
     1261 atgcctaaaa gaatgatgca ggatagcact ttcgaggatt atacagaggc ttgcagttat
     1321 gaggaggcac tgagcgaggt gcacaatgga gtttatcagg ctctgtttta tgtcgctgca
     1381 atcgtttcaa gaaaggaaaa gagcagcgga gagagagagt cttttgatga ttataaccag
     1441 gaggagcgag ctgagttttt taaacagcat ctctctgctt ttgatgcttt tcagataggt
     1501 ttttttttgc gcaggctaag cgagcaatcg ctgacagatt tccaaatctt tacagcggcc
     1561 tcgaccctga gcagattaaa gctggttaca aacaactgat tgagcctttt ggatggttta
     1621 atacgtttgt aagtttggca ggagatgaca tcctccgatt ggaggcaata aataaaatat
     1681 cactctggca ggcgatggca tttttaagcc agcaggcagc aaaagcagag tatcaaagta
     1741 acctaatgaa accgaaccaa taatgttact aatacaagta tctaatatat tgaatgattt
     1801 tgtccaggct gatcctgata ttaacttttt ccactgtgga ctccctaacg atgtgaatgt
     1861 taatatcccg aataattacg accctaacag ccgtatcggg aaccagtttc cctatgttct
     1921 gctcatccct ccgattgtgc agggacgagt cgaggcaaat ggagagcagg gtatttttga
     1981 gacttttcca gttgatattt taatctctga tttgtatggg caaacgaact ccggaatata
     2041 tggtactaat acctatgctt tactactttc aaagctgcag gttatcgctc agcgtattgt
     2101 taaagcgttt caaagctatg cagaatatgc aagtcctcag tttaatgtag gtgattatac
     2161 tctaacattt gatagtctga gacatacaca aaacactcgg agcctccggc tacagttcga
     2221 tttaattttc gcactttcct gcagtgatta tactatagat ttgagtacct ttgcaactga
     2281 tgcaggagac actgtaagat atgacaaaga ggatcctatt aacacaactc cagtc
//
LOCUS       DBNGSV010000051        10481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1463970, whole genome shotgun sequence.
ACCESSION   DBNGSV010000051 DBNGSV010000000
VERSION     DBNGSV010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1463970"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC159U_01860"
     CDS             complement(<1..352)
                     /locus_tag="AC159U_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3335834.1"
                     /translation="MKWSWKIGNFSGIDLKVHATFAIIIVWVLYNTITAGDDIWMTIE
                     TLLFVLTIFACVVLHEYGHALTAKKYGIKTLDIILLPIGGVARLEKMPDDPKQELWVA
                     LAGPAVNVVIAFIIF"
     gene            522..1235
                     /locus_tag="AC159U_01865"
     CDS             522..1235
                     /locus_tag="AC159U_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3335835.1"
                     /translation="MKRAALITGGAKRIGKEICIYLAEKGYDIALHYNTSESKAKSLS
                     KQIKKSGVECRLFKSDLSKPKFSASLILKVFRQYPHCSVLVNNASVFKEKEFSEITLK
                     DFENDMAINFTSPFFLTQQFSKHKSASNIINLIDTRVSRVNSTYFTYNLSKYCLYKFT
                     KMSARQLAPKIRVNGICPGDILPLEGLDKSYLKKRSKRLPLKRVGCVGNVIAAMDYLL
                     NNEFVTGQCLFVDGGEHLL"
     gene            1247..1594
                     /gene="folB"
                     /locus_tag="AC159U_01870"
     CDS             1247..1594
                     /gene="folB"
                     /locus_tag="AC159U_01870"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011072826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3335836.1"
                     /translation="MILRIENLRLRTIVGIYDWEKENKQDLVVNVELEFDGSKAARSD
                     NIDDSVNYKTINKQIIEHIENGNFDLIEKIAGDILDIIFKNDKVEKATVKVDKPGALR
                     FTDSVSVQLSREK"
     gene            1619..2002
                     /gene="folK"
                     /locus_tag="AC159U_01875"
     CDS             1619..2002
                     /gene="folK"
                     /locus_tag="AC159U_01875"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3335837.1"
                     /translation="MNRVIVAIGSNINPYENVKRAKELISLQHKVIRSSKFIMTKPIG
                     YTAQDDFLNGGLLVHTNFDKKPFKSYLRDIEDKLGRERTENKYGPRTIDLDILIWNGT
                     VVDKDYYDRDFLQSIVKELDVELYK"
     gene            2109..2927
                     /locus_tag="AC159U_01880"
     CDS             2109..2927
                     /locus_tag="AC159U_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR3335838.1"
                     /translation="MKKIDTLILIFAGLGLLISAYLSYTYFSSGQTTFCLSGSGCDVV
                     KESPYSKVFGISVPYYGLFGNLIIIGSLLYKGLGGKKYSIIYYLSLAGVAFSAYLTYL
                     EVAVIKAICSYCVLSAVFILAIFLTVLISKQLKSLNTAGIRFILTSFVVFLIVIGAAY
                     FSHSDLRASAPASKAVIDLAKHLKGQNSSMYGSYTCPHCNTQKLMFADAFKYINYVEC
                     NPGGENPNVKLCEEKGIDGYPTWEINGKLHPGAKSLRELAKLSGFKQARQASDK"
     gene            complement(2932..3549)
                     /locus_tag="AC159U_01885"
     CDS             complement(2932..3549)
                     /locus_tag="AC159U_01885"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3335839.1"
                     /translation="MIKLYDFPRCPYCRKVRIALVEKQIPYEQIFVDLNKKEQRKPKF
                     LKLNPNGKVPVIADGSKIIYESTIINEYLDEKYPLRPLLPKELYTKALTRMLIEYCEK
                     TFHTYVFGIYKELKLKNNLKANQKLIELNLKNLDTELKYLNGILKGHNYITGRFSLAD
                     VAFMPRILLLDYLDIHINSKYKYVLNWIDKLKIRESSKIVLEGNL"
     gene            complement(3594..3731)
                     /locus_tag="AC159U_01890"
     CDS             complement(3594..3731)
                     /locus_tag="AC159U_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335840.1"
                     /translation="MPPAIFNFVLAEKYKQNSQAVASIILAGTLISVFTLPVIVAYLI
                     R"
     gene            complement(3712..4473)
                     /locus_tag="AC159U_01895"
     CDS             complement(3712..4473)
                     /locus_tag="AC159U_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR3335841.1"
                     /translation="MQILTILNIVFPVFILSLLGFIFGRFKKINLPSLNEFVIYIAMP
                     CLILSSLSKKHIELTVAGNVFVALVIIMILSAAITVVILKKLNLDSGTYLPTVLFANT
                     GNMGLPIVYYAFGQDGLTLAILYMVCTTFIHYTVGILLFNIKNGPTELLKLPLIYATI
                     AGLTISFLNIDLPVILSRTLQLAGEASIPTMVFALGYKLSEIKLTHISRSILFGVLRI
                     LLGFSFGLLAVKLLKIEGLYSKCNYTSGIHAPCHF"
     gene            complement(4538..5515)
                     /locus_tag="AC159U_01900"
     CDS             complement(4538..5515)
                     /locus_tag="AC159U_01900"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3335842.1"
                     /translation="MSSVRILSTGTSIPSKVITNSYLEKIVDTNDEWITARTGIKERR
                     MADENHASSDLGAEAAQKAIDKAAIDPMDIDVVIVATITPDYLFPSTACLIQKTIGAD
                     NAYAFDLSAGCSGYLYAMQVAKGMIDGGCAETILIIGAETLTRVTDYEDRGTCILFGD
                     GAGATILQKSDERGILSICLGSDGGGWDLLYQPGGGSRIPSSVQSVENKEHYLKMNGK
                     EVFKIAVTSMGNASVQAIEKAGIKPEDIDMFIAHQANYRIMDAVRKRLKMPTEKVFMN
                     ISKYGNTSSASVPIALDEALEQGKIKKGDLILFSVFGAGFTWGASVIEW"
     gene            complement(5578..6015)
                     /locus_tag="AC159U_01905"
     CDS             complement(5578..6015)
                     /locus_tag="AC159U_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335843.1"
                     /translation="MANFIDKQEIDLPKYIKSELKFIEKGIVSHKIEGKIYFIFKNNA
                     KSRVNLDDIEEISVEFDPILRDEFPSVRMIIHFSDNYDELFNMDYYFSIESDKELEHL
                     YDLYGSKIIQIIYYDKETSVCCNYSLNMDELKTISNTIEQIES"
     gene            6200..6394
                     /locus_tag="AC159U_01910"
     CDS             6200..6394
                     /locus_tag="AC159U_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335844.1"
                     /translation="MGGTFITLLIVFLVLGVFFKMGPEEEAPAEEAAAEPEAAPAADA
                     PAPPEPPAGSGGEQDAFKAP"
     gene            complement(6474..8036)
                     /locus_tag="AC159U_01915"
     CDS             complement(6474..8036)
                     /locus_tag="AC159U_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3335845.1"
                     /translation="MNGKTIPTYKTKPLISTETWILVATILGSSMAFIDSTAMNVVLP
                     VFQLELNAKISQVQWVIESYALFLASLLLLGGALGDHYGRKKIFAIGVVIFSLSSLWC
                     GLSPDIHQLIAARAFQGIGSAVLVPGSLAIITSSFRQEKRGKAIGTWAAFTALMTALG
                     PLLGGWLAEHISWRWVFFINVPISVLVIITLIKFVPEIKNTEQKSKLDLVGGLLITLS
                     LGCIVFGLVESSNHGFSNPFILPSIIIGIIFFGVFIYHEKKVENPLVPLDLFKSTVFT
                     GGNIVTFFLYSALSGAIFFVPFNLVQIQGYSATQAGAAFLPLLIILFLLSRWSGSIVD
                     KYGAKPPLVIGPIISAVGYLMLTEIDMSISNYWNSFLPAMIVLGLGMSATIAPLTTAV
                     MNAANVEHSGVASGINNSVGRIAGVLSIAVLGLFMFTTFSGNINKELQTSSLPAEIKE
                     SIANQTTKLAAMSISDELDENTRSQIKQIINKSYNSSFILIMYLITGLALLSSFVAFK
                     TMKPKTKKDNTT"
     gene            complement(8202..8783)
                     /locus_tag="AC159U_01920"
     CDS             complement(8202..8783)
                     /locus_tag="AC159U_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR3335846.1"
                     /translation="MNITVFYDYICPFSFIGSKRIQKIADEYGIELEWKGFEIHPEYP
                     PDGKLRKKSLKMARVNESLSSVMEEEESKFKLPGFVTNTNMALQAAEYAKSEGKFIQF
                     HNSCYDSYMLERKNIGDINIILEIAETIGIDKHLLENTLKNKEMEENLATYKVQAENA
                     DVLGVPTILFNDFRIHGVQSLETYRSIIKKFSN"
     gene            8830..9174
                     /locus_tag="AC159U_01925"
     CDS             8830..9174
                     /locus_tag="AC159U_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335847.1"
                     /translation="MSVRIVEEELEARLVPVEMSASPSTPKAKAESKVYGDGNRRFKL
                     SMDNLDVPRGEDVEFYINGELLLRIQIDKGKAKLDLRSENGDSIPVVSANDVAYIRYS
                     DNVLVKGIFYAD"
     gene            9209..9706
                     /locus_tag="AC159U_01930"
     CDS             9209..9706
                     /locus_tag="AC159U_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3335848.1"
                     /translation="MNQKNSDKEHPDNANVMVFPPFIYLGFFIMGYLLHYFFKVLIVN
                     QANLASILGIIGIALIIVSIIFPLLAIKTLGKSKTTHMVSEPTTAIVSSGVFKYSRNP
                     IYLCGITLYLGISLIINSLLLLISIFPLFYAIREGLVKREEQYLERKFGQEYLNYKER
                     VRRWI"
     gene            9713..10147
                     /locus_tag="AC159U_01935"
     CDS             9713..10147
                     /locus_tag="AC159U_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335849.1"
                     /translation="MWFIYLIYPVGLFALALVLILCKYFYNVLSNNSQILEISSSSKA
                     KFYFYSAINLIVMIVWILFAVESSPLKNRVSDYGAPYGGLALLLIIPVSLVISTYINT
                     KTANKNLEYYRYIAVSKLLWLISLIPGIIVVLFYGLYMFSGK"
     gene            10270..>10481
                     /locus_tag="AC159U_01940"
     CDS             10270..>10481
                     /locus_tag="AC159U_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335850.1"
                     /translation="MKKQNIFFLVFGLFLFLAQGALAQDFYVSTTGSDSTGDGSLGNP
                     WATITNALDNAPDDGSVILVQPGIYNG"
BASE COUNT         3329 a         1783 c         1931 g         3438 t
ORIGIN      
        1 caaaaataat aaaagctatg actacattta cggcaggccc ggcaagggct acccagagtt
       61 cctgtttggg atcgtcaggc attttttcaa gccttgctac tccgccaata ggcagcaata
      121 tgatatccag agtttttatg ccgtattttt ttgcagttag cgcatgacca tattcatgta
      181 gtactacgca ggcaaagata gttagtacaa ataacaatgt ctctattgtc atccatatgt
      241 catcacctgc ggttatggtg ttgtagagca cccatacaat tattattgca aaagttgcat
      301 gaaccttaag gtcaattcca ctaaaatttc ctattttcca agaccacttc atatttgtat
      361 atccggtgtt ttattatagt tttgtattac aaaagataat aggtaaataa tttattacaa
      421 gattatccat atggcttata gtttaaatga taagccattt aagataatta atgtactaaa
      481 agataaaatt tgctaaactt aaaacacttg agaattgtac catgaaaaga gcagctttaa
      541 ttacgggtgg ggcaaagagg ataggaaagg aaatatgtat ttaccttgct gagaagggct
      601 atgatattgc tctgcattat aatacatctg agagcaaggc taaaagtctg tctaagcaaa
      661 taaaaaaatc aggtgtggaa tgcaggttat tcaagtccga tttgtctaaa cctaaattct
      721 ctgcatcttt gattttaaag gtatttagac agtatccaca ctgttcggtt ttggttaaca
      781 atgcctcggt ttttaaagaa aaagaatttt ctgaaataac actcaaagat tttgaaaacg
      841 atatggcaat taatttcaca tcaccttttt ttctcactca gcagtttagc aaacataaaa
      901 gcgcatctaa tattattaac ctcattgata caagagtatc tagggtaaat agtacatatt
      961 ttacgtataa tctaagtaag tactgtctgt ataagtttac aaagatgtct gccaggcagc
     1021 tagctcccaa aataagagtt aatgggattt gccccggtga tatacttcct ctcgagggtc
     1081 ttgataaaag ctatttgaaa aaaagatcaa aaagactacc cttaaaaagg gtaggatgtg
     1141 ttggtaatgt gattgctgct atggattatt tactaaataa tgaatttgtt accgggcagt
     1201 gcttatttgt tgacggcggg gagcatcttt tataaggaat taatatatga tactgcgaat
     1261 agaaaattta agactgagaa ccatagtagg gatatatgac tgggaaaaag aaaacaaaca
     1321 ggatttagtg gtaaacgtag agcttgaatt tgatggttca aaagcagcca ggagtgataa
     1381 tattgatgac agtgtaaact ataaaaccat aaataaacag ataattgaac atatagaaaa
     1441 cggcaatttt gatttgatcg aaaagatagc aggggatata ttggatataa ttttcaaaaa
     1501 tgacaaagtg gaaaaagcta ctgtgaaagt agacaaaccc ggagctctta ggtttacaga
     1561 cagcgtatca gtgcaactga gcagagaaaa ataaatactc aaaaggtgta tatatagctt
     1621 gaaccgtgta attgtagcaa ttgggtcgaa tattaatcct tatgagaatg taaaacgagc
     1681 aaaagaactt atatctttac agcacaaggt cattagaagc tctaagttta taatgacaaa
     1741 acccataggc tacacagctc aggatgattt tttaaatgga ggtctgcttg tacatacaaa
     1801 ttttgataag aagccattta aatcatattt acgagatata gaagataagc ttggcagaga
     1861 gagaacagaa aataaatatg ggccgagaac tatagacctc gatattctaa tatggaacgg
     1921 cactgtggtt gataaggatt attatgatag ggattttttg cagtcaatag taaaagagct
     1981 ggatgttgag ttatataagt aggtataatt aggtatttaa ttttatgaaa aatttttatt
     2041 aatagaatta aaatataata tataatctta attgcatcag attctaattt atttgcatca
     2101 aacaaaaaat gaaaaagatt gatacattaa ttctgatatt tgccgggttg ggcctgttaa
     2161 tttctgcata tttgtcctac acatattttt ccagtgggca gactacgttt tgtctttcag
     2221 gatcggggtg tgatgtagta aaagaaagtc catattcaaa agtatttgga atttcagttc
     2281 cttattacgg attatttggt aatttaataa ttattggctc tctcttgtat aaaggtcttg
     2341 gcggtaaaaa atatagtatt atttactatt tgtctcttgc cggagttgca ttttcagctt
     2401 acctgaccta tttggaagta gctgttatta aagcaatatg ctcatactgc gttttatctg
     2461 ctgtttttat attggctatt tttcttacgg ttctaatatc aaagcagtta aagtcgctca
     2521 acacagctgg aattagattt attcttacaa gctttgtagt ctttttaatt gttattggag
     2581 cggcatattt ttcgcattct gacctgaggg catcagcccc tgcttcaaaa gcagttattg
     2641 atttggcaaa acatctaaaa ggccagaata gctcaatgta tggctcatac acctgcccgc
     2701 attgcaacac tcaaaaactt atgtttgcag atgcgtttaa atatataaac tatgtggaat
     2761 gtaacccggg gggtgaaaat cccaatgtga agctttgtga agaaaaaggc attgatggtt
     2821 atccaacatg ggaaattaac ggtaagcttc atccgggggc aaagtcactt agggagcttg
     2881 caaagctttc aggatttaag caggcaaggc aggcttctga taaatagagg attaaagatt
     2941 tccttctaat actattttgg agctctctct tattttaagc ttatctatcc agtttagaac
     3001 atatttatat ttgctgttaa tatgaatatc cagataatca agaagaagaa ttcttggcat
     3061 aaatgctaca tctgcaagac tgaatcttcc tgttatatag ttatgccctt ttagaatacc
     3121 atttaaatat ttaagctcgg tatcgagatt tttcaaatta agttctataa gtttttgatt
     3181 tgcctttaaa ttatttttaa gttttagctc cttataaata ccaaatacat atgtgtgaaa
     3241 ggtcttctca cagtattcta tcagcattct cgtaagggcc ttggtataga gttcttttgg
     3301 tagtaggggt cttagtggat acttttcatc aagatactca tttattattg tagattcata
     3361 aataatcttt gatccatctg ctatgacagg aacctttcca tttgggttta gttttaagaa
     3421 ttttggtttt cgttgttctt ttttattcag atcaacaaaa atttgctcat atggtatctg
     3481 tttttctact agtgcaatcc gtacttttct gcagtatggg catcttggaa aatcgtaaag
     3541 cttaatcatt tttgttagta ataattatag taatcataaa atatagtaaa caattatctt
     3601 atcagataag caacaataac aggaagagta aatacggaga tcagcgttcc agccaaaatt
     3661 attgatgcca ctgcctgaga attttgtttg tatttttcag ctagaacgaa gttaaaaatg
     3721 gcagggggca tggatgcctg aagtataatt acatttgcta taaagacctt caatctttaa
     3781 tagttttact gctaacaagc caaaggaaaa cccaagtaat attcttagaa ctccaaatag
     3841 aatagatctg ctaatatgtg ttaattttat ctcagagagt ttgtacccca gggcaaagac
     3901 cattgttggt atgcttgcct cacctgcaag ctgcagcgtt cggctcagta ttaccggtaa
     3961 atcaatattt aaaaagctga tagtaagacc tgcaatcgtt gcatagatta aaggcagttt
     4021 gagcagttct gtagggccgt ttttgatatt gaataatagt attcccacag tgtagtggat
     4081 gaatgtagta cagaccatat ataaaatagc tagtgtgaga ccgtcctgcc caaatgcata
     4141 atacacaatg ggcaggccca tatttcctgt gtttgcaaaa agaacagtgg gtagatatgt
     4201 gccggaatcc agatttagtt ttttaagtat tacgactgtg atagccgcac ttaaaatcat
     4261 tattattaca agagctacaa aaacattacc ggctacagta agctcaatgt gctttttgga
     4321 cagagaagaa agaataaggc agggcatagc aatgtagatg acaaattcat ttaaagaggg
     4381 caggttaatc tttttaaatc ttccaaatat aaatcccaga agcgaaagta tgaaaacagg
     4441 aaaaacaatg tttaggatgg taagaatctg aatgtcctta tgttagtttg ttaatgaaat
     4501 ggaaatatta aaactctaat taaagatgtt gcaaaattta ccactcaata actgatgcgc
     4561 cccaggtaaa ccctgctcca aatactgaaa acaggatgag gtctcccttt ttaatctttc
     4621 cctgttccag cgcttcatcc aaagcaatcg gtactgaagc tgaagaagta tttccgtact
     4681 ttgaaatatt catgaatact ttttctgtgg gcatcttcag tcgttttctt acagcatcca
     4741 taattctata atttgcctga tgagctatga acatgtctat atcttctggt ttgatgcctg
     4801 ctttttctat tgcctgtacc gaggcattgc ccatcgatgt tactgctatc ttaaacactt
     4861 ccttgccgtt catcttaagg taatgctctt tattctcaac gctttgcact gatgatggta
     4921 ttctcgatcc cccgccgggc tgataaagaa ggtcccagcc ccctccgtct gatcccaggc
     4981 agatagaaag tatcccacgc tcatcagatt tttgtaatat agttgcgcca gcaccatcac
     5041 caaagagaat acaagtaccc ctatcttcat aatcggttac tcttgtaagg gtctcagcac
     5101 caattatcag tatagtctct gcacatccgc cgtctatcat accctttgcc acctgcatag
     5161 catataggta accggagcat ccggctgaaa gatcaaaggc ataagcattg tctgccccga
     5221 ttgttttttg gataaggcag gctgtagacg gaaagagata gtcaggtgta attgtagcaa
     5281 ctatcacaac atctatatcc attgggtcaa tagcagcttt gtctatagct ttttgagctg
     5341 cttcagcgcc aaggtcagaa gacgcgtgat tttcatctgc catcctcctc tctttaatcc
     5401 ctgttcttgc agttatccat tcatcattgg tatctacaat tttctcaaga taagaatttg
     5461 ttataacctt agatggaatg gatgtccctg tacttaatat tcttactgaa ctcaattttt
     5521 gctgtctccc ctattcaaat gtggctaatt atctttgaat gagataggtt tgtcaaccta
     5581 actctcaatc tgctcaattg tattactaat agtttttagt tcatccatat ttagagaata
     5641 attgcagcaa acagatgttt ccttgtcata gtagataatt tgtatgatct tagagccata
     5701 taaatcatat agatgctcca gttccttgtc ggattcaatt gaaaagtaat agtccatatt
     5761 gaaaagctca tcataattat cggaaaaatg gataatcatt cgcaccgatg ggaattcgtc
     5821 cctgagtata gggtcgaatt ctactgaaat ctcttcaata tcatcaagat ttactctact
     5881 tttagcattg tttttgaata taaagtaaat ttttccttca attttatgac taactatacc
     5941 tttttcaata aatttcaact cagatttgat gtatttaggt aaatctatct cctgtttatc
     6001 aataaaattt gccattttat gagattaaat tagtgattat attaaattat agtggtttta
     6061 tgatgatctg aaagaaaaat taatcaaatt atttgtccgt ttaagaaaat tctccttgac
     6121 aattatatat aaaatttatt actatcggag tataaggtta agatagaatt atatttttta
     6181 gacataagga ggagaaatta tgggtggtac ctttattact cttttaatag tttttcttgt
     6241 attaggggtt ttctttaaaa tggggcctga agaagaagca cctgcagaag aggctgcagc
     6301 cgagcccgaa gcagctccgg cagctgatgc tcctgctcca ccagagccac cagctggttc
     6361 aggcggcgaa caggacgctt ttaaagctcc ttaataagta tgatttaatc cataaaagga
     6421 ttaaatagtt tgttaattat aaaactggac tacaatgtag aggcaacact tggttaagta
     6481 gtgttgtctt tctttgtttt tggcttcatt gtcttaaatg ctacaaaact actaagcagt
     6541 gcaagccctg ttattaaata cataatcaaa ataaaactcg aattataaga tttattaata
     6601 atttgtttta tttgagacct tgtgttttcg tccagttcat ctgaaataga cattgctgca
     6661 agttttgttg tttgatttgc aatggactct ttaatctctg caggtaagga tgaagtttga
     6721 agttctttgt taatatttcc actaaatgta gtaaacataa acagtcctag tactgcaatt
     6781 gagagcaccc ctgctattct tccaacagag ttattaattc ccgaagctac tcctgaatgt
     6841 tctacatttg ctgcgttcat tactgctgtt gtaagtggag caatagtggc tgacatacca
     6901 agtcccaaaa ctatcattgc cggcaaaaat gaattccagt aatttgatat actcatatca
     6961 atttcagtta gcattaaata tcccacagct gagattatgg gtccgataac aagaggcggt
     7021 tttgcgccat atttatctac tatactcccc gaccaccttg ataaaaggaa taaaattatt
     7081 aacagcggca aaaacgctgc acctgcctgg gtggctgaat atccctggat ctggactaag
     7141 ttgaatggta caaaaaatat agctccgctg agggctgaat aaaggaaaaa agttacgata
     7201 ttgccgcctg tgaagactgt actcttaaac aggtcaagcg gaactagagg gttttctact
     7261 ttcttttcat ggtatatgaa tacaccaaag aatataatcc caattattat agagggtaga
     7321 atgaatggat tgctaaaccc atgattcgac gattcaacaa gtccaaagac gatacatcca
     7381 agtgagagtg ttattaacag ccctcctaca agatcaagtt tagatttttg ctcagtattt
     7441 tttatttcag gcacaaattt aattagtgtg ataatcacta gaactgaaat cgggacatta
     7501 ataaaaaata cccatctcca ggatatgtgt tctgcaagcc atcccccaag cagtggtcca
     7561 agtgctgtca tgagtgctgt aaatgctgcc catgtgccaa ttgctttacc tcttttttcc
     7621 tgcctgaaac tcgaagttat tatagcaagg cttccgggca ctaaaacagc acttcctata
     7681 ccctggaatg cccttgcggc tattagttgg tgaatatcgg gggaaagccc acaccaaagc
     7741 gatgacagag agaagataac tactccaata gcaaatattt tttttcttcc atagtggtcg
     7801 ccaagtgcgc ctccaagaag aagaagtgat gcgagaaaca gtgcataaga ttctatcacc
     7861 cactgaacct gtgaaatttt tgcattaagc tcgagctgga aaacaggcag taccacattc
     7921 attgccgtac tgtctataaa cgccatgctt gaaccaagaa ttgtggcaac cagtatccat
     7981 gtttcagtac ttataagtgg tttagtcttg taagtgggga tcgttttgcc gttcatataa
     8041 gtatttagat attaaataat gagattagat tatttgattg ggaattgaaa aatattatag
     8101 tgtctggtca gcagctctca gtactgtatt tattactcat ttgatgataa atggatgaga
     8161 tttattgtca atttgagttt tatattaacc ccaaatcaat tttagtttga aaatttcttg
     8221 attatggatc tgtaagtttc caggctttga acaccgtgaa ttctaaaatc gttaaagagt
     8281 attgtgggta cgcctagtac atctgcattt tctgcctgca ctttataggt agctaaattt
     8341 tcttccatct ctttattctt aagagtgttt tcaagcagat gtttatctat tcctatagtt
     8401 tctgcaattt ccagaataat attaatatcc ccaatgtttt ttcgctcaag catataggag
     8461 tcatagcagc tgttgtgaaa ttgaatgaac tttccctcag attttgcgta ttctgcggcc
     8521 tgaagggcca tattggtgtt cgttacaaat ccaggaagtt taaatttact ttcttcctct
     8581 tccataactg aacttagcga ttcatttact cttgccatct tgaggctttt tttcctgagt
     8641 ttgccatctg gcggatattc aggatgtatt tcaaagcctt tccattccag ctcaattcca
     8701 tactcatctg cgatcttttg aattcttttt gagcctataa atgaaaacgg gcatatgtaa
     8761 tcataaaata ctgtaatgtt catggctaaa tgttaaaata actctaaaat tacctaattc
     8821 aaaataaata tgagtgttcg aatagtagaa gaagagcttg aagccaggct ggtacctgtt
     8881 gaaatgtctg catcaccatc cacacctaaa gcaaaagcag agagcaaagt atatggagat
     8941 ggtaacagga ggtttaagct ttctatggat aatttagatg taccaagagg agaggatgtg
     9001 gaattttata taaacggtga attgctgtta aggattcaaa tcgataaggg taaagcaaaa
     9061 cttgatttga gatctgaaaa tggagactca attcctgtgg tatcagcaaa cgatgtcgcc
     9121 tatattcgtt atagtgataa tgttttagtt aaaggtatat tttatgcaga ttaatattag
     9181 aaaatattaa tgctttttaa ataattgtat gaaccaaaaa aactccgata aagaacatcc
     9241 tgataatgcc aatgtgatgg tattcccacc ctttatttat ctaggatttt ttataatggg
     9301 atatttattg cactattttt ttaaagtgtt aatagtgaat caagctaatc tagcttctat
     9361 cctgggtatt ataggtatag cattaattat agtttctata atattccctt tgttggctat
     9421 taaaactctg ggaaagtcta aaaccacaca tatggtttca gagccaacca ctgcaattgt
     9481 cagcagcggt gtattcaagt attcaagaaa tcctatttat ctatgtggaa ttactttgta
     9541 tcttggcatc tcattaatca tcaattccct tttgttgcta atatcgatat ttccactttt
     9601 ttatgcaata agagaagggc tggttaaaag agaagagcaa tatcttgaaa ggaaatttgg
     9661 gcaggaatac ctaaactata aagaacgggt aaggagatgg atataggctg aaatgtggtt
     9721 tatatatcta atatatcccg ttggactatt tgcacttgct ctggttctaa tattgtgtaa
     9781 atatttctac aacgtattat caaataattc tcaaatcctg gaaataagca gcagttcaaa
     9841 agccaaattc tatttttatt cagcaatcaa tttaatagta atgattgttt ggattttatt
     9901 tgcagtggag agctcaccac ttaaaaaccg ggtatctgat tatggagctc cttatggcgg
     9961 gttagccctc ttattgataa ttcctgtaag tctagtaatt tctacctata tcaacacaaa
    10021 aacggccaat aagaatcttg agtattatcg ctacattgca gtatcaaaac tgctctggct
    10081 catttcatta atacccggaa taatagtagt tttattttat gggctttata tgttttcagg
    10141 caagtaaagt catattttac tacaacaata attcaccttt actaattcac acacaaacca
    10201 aataagacct attgactaat aatacaaaag tattacaatt tagtgcatgt caatttcgta
    10261 ggggtgaaga tgaaaaagca aaacatattt ttcttagtgt tcggtctatt tctttttctt
    10321 gctcaaggag cattagcaca ggatttttat gtatcaacca cagggagtga ctctactggt
    10381 gacggctcac ttggtaatcc ctgggccaca attacaaatg cattggataa tgcccccgat
    10441 gacggcagtg taattttggt acaaccgggg atatataacg g
//
LOCUS       DBNGSV010000052         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_841259, whole genome shotgun sequence.
ACCESSION   DBNGSV010000052 DBNGSV010000000
VERSION     DBNGSV010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_841259"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC159U_01945"
     CDS             complement(<1..258)
                     /locus_tag="AC159U_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335851.1"
                     /translation="MKTIYIPNESFADSNDVKVANKLLSRLADNLLDEIDDNIEVWII
                     GNKDKDFFNKQINIPMKKRRVLDPFDQLPISDFRQLHHVSIV"
     gene            complement(516..1577)
                     /locus_tag="AC159U_01950"
     CDS             complement(516..1577)
                     /locus_tag="AC159U_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-hypusine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HIR3335852.1"
                     /translation="MSEKNLFQQKVNAIEVGNTKTVSQLLEQMGNTGFQGKSLARVVD
                     VYEDMIKDEDVTILFGYTGSLSTTGQWKIINWLIENRYIDILIPTGANISEDLVDAMG
                     MGYWQGDSNENDEMLFEQGVNRYYDVYGKESDYLKMTELIAEFIVTLEDGYKYSSREF
                     LKHFGNWLLGKDINSIVATAAKHDVPIFCPAIADSPYGDAALIAKSRGFDLTIDAIKD
                     YVEFMQFSEGVKQTGVVYIGGGVPKDFIQLFAVTADLLYKDRKVPNRPGGNTRKEVGD
                     LETYYPHKYAIQITTDSPQWGGLSGCTFSEAVSWGKEQPDGGLVQCYCDATIALPLVT
                     QALSERLGTKRKGTVFTNI"
     gene            complement(1609..>2524)
                     /locus_tag="AC159U_01955"
     CDS             complement(1609..>2524)
                     /locus_tag="AC159U_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3335853.1"
                     /translation="PEKVKYWLSVDQYIGGIEHAILHLLYSRFFTKVLRDLGFSDIDE
                     PFKNLLTQGMVIKDGAKMSKNIGNVVDPDDMIIKYGADTVRVFMMFASPVQRDLDWSD
                     EGVEGSYRFLNRVWRIVYENIDLLKSSEDIVTKLDGLSKGAKELKIQVHKTIKKVTDD
                     LEKFQFNTSIAAVMELLNSVSKFEPESDTDKSVISEAINSIIRLLYPIAPHFSQELWS
                     VTGKESSLVDLQWITWDEKIVRSASVTVVFQINGKLRSQLDMDPESSDDELKAAAYAD
                     ERIKSYIDGKEIRKVIVVPKRLVNIVVG"
BASE COUNT          726 a          550 c          374 g          874 t
ORIGIN      
        1 gactatagat acatgatgta actgtctaaa atcagatata ggtagttgat cgaacggatc
       61 taaaaccctt cgctttttca taggaatatt aatctgcttg ttaaaaaagt ccttgtcttt
      121 gtttcctatg atccagactt ctatgttgtc atcaatttca tccagtaaat tatcagctaa
      181 gcgtgataaa agcttattgg cgaccttgac gtcgttactg tctgcaaaac tttcgtttgg
      241 aatataaatt gttttcatca gttcccccct gattaataat atacgacttt aatagtagta
      301 tatgcaaaag ttataccatt gagaaaaagt attgatcagg taatgattaa agtaagttac
      361 aaaataattt aataagagtt ttactatata agtcgtatat attatgacaa ttattgaaca
      421 gtaatgtcat ttcttgacag gtatatcaca catacatgca ataaagttta atatatttgt
      481 atgagtatga atatttataa cttttgctat gtagtctata tattggtaaa gactgttcct
      541 tttctctttg tacctaatct ttcagaaagt gcctgtgtta caagtggcag tgcgattgta
      601 gcgtcacaat aacattgtac aagccctcca tcaggctgct cttttcccca cgatacggcc
      661 tcactaaaag tacatcccga tagacctccc cactgaggag agtctgttgt aatttgtatt
      721 gcgtacttat gtggataata ggtttcaaga tcaccaactt cttttctcgt atttccacca
      781 ggtctgtttg gtactttcct gtctttatat aaaagatccg ccgtgactgc aaatagctga
      841 atgaagtctt ttggaacacc gccgcctata tatacaaccc ctgtttgctt cactccctca
      901 ctaaactgca taaactccac ataatctttt atcgcatcta tagtaagatc aaaccccctg
      961 ctctttgcaa taagtgcagc atcaccatat gggctatctg ctattgcggg gcaaaaaata
     1021 ggcacatcgt gtttagccgc tgttgcaacg atactgttaa tatccttgcc aagcagccag
     1081 tttccaaaat gctttaaaaa ctctctggat gaatatttat aaccatcttc taaggtaaca
     1141 ataaactcag ctattaattc agtcattttc agatagtcac tttctttacc gtaaacatcg
     1201 taatacctgt ttactccttg ttcaaacaac atctcatcat tttcgttaga gtctccctgc
     1261 cagtagccca ttcccattgc atccacaagg tcttctgaaa tatttgcgcc tgtaggaata
     1321 agaatatcaa tgtatctgtt ttcaataagc cagtttatta tcttccactg tcctgttgtt
     1381 gatagtgacc ccgtatagcc aaagagtatg gtaacatctt cgtcttttat catgtcctca
     1441 tatacgtcca ctaccctggc aaggctcttt ccctgaaatc cggtgttacc catctgttct
     1501 aataactgag aaaccgtctt tgtatttccg acctcaattg catttacttt ttgttgaaat
     1561 aagttctttt cgctcatgtt tttccttaaa ataataatta tttaattttt atccaacaac
     1621 aatatttaca agcctctttg gtacaacaat aacctttcta atttctttac catcaatata
     1681 gctttttatc ctttcatcag catatgctgc agcctttagc tcatcatcgc ttgactccgg
     1741 atccatatcc aactgacttc ttagctttcc atttatttga aatactactg ttactgatgc
     1801 gctccttaca attttttcat cccacgtgat ccactgcaaa tccaccagtg agctctcttt
     1861 acctgttaca ctccaaagct cctgggaaaa atgtggagct attggataca aaagcctgat
     1921 aattgagtta atagcttctg aaattacact tttatctgta tccgattcgg gctcaaattt
     1981 tgaaaccgag ttaagcagtt ccattacagc agctatagat gtattaaact gaaatttttc
     2041 cagatcatca gtaacttttt ttattgtttt atgaacctgg atttttagct cttttgcacc
     2101 cttactcagg ccatccagtt tagtaactat atcctcactg gattttagta gatcaatatt
     2161 ttcgtataca attctccaga ctctgtttaa gaatctgtat gatccttcga caccttcatc
     2221 actccagtcg aggtctcttt gcaccggtga ggcaaacatc ataaatactc ttacggtatc
     2281 ggcgccgtat tttattatca tatcatcagg gtcaactaca ttgcctatat ttttagacat
     2341 ctttgccccg tcttttatca ccattccctg agtaagcaga ttcttaaatg gctcatcaat
     2401 atctgaaaac cccaaatccc ttaaaacctt agtaaaaaat ctggaatata acaaatgcaa
     2461 aatcgcatgt tcgattccgc caatatactg atctacggag agccaatact ttactttttc
     2521 tgga
//
LOCUS       DBNGSV010000053         2151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_217822, whole genome shotgun sequence.
ACCESSION   DBNGSV010000053 DBNGSV010000000
VERSION     DBNGSV010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_217822"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC159U_01960"
     CDS             complement(<1..502)
                     /locus_tag="AC159U_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335854.1"
                     /translation="MKENVKRIGDGVLYIASGKTYTKAAINSAITVKKYSPKLNIHIF
                     VDKKQTELVKEYGDIFSSFGIINNPHYRSKVDYLTHTPFSKTLYLDSDTKICDSIYDI
                     FELLERFDIALAHAHKRNSPKTTVSWTQDIPLCFPQYNGGVIAYNSCKEVIDLLDSWK
                     SSFHDAS"
     gene            complement(655..1530)
                     /locus_tag="AC159U_01965"
     CDS             complement(655..1530)
                     /locus_tag="AC159U_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3335855.1"
                     /translation="MSAHPPTIIIGMSRSGTGILAKMLEDLGLFIGKSKDRNHEALFF
                     IDLNMWLLEQSSGGLENPQSIKYILEDKEVRTLFAEFINHTMKTPKAISYLGFKKYLS
                     HRTPANLDIPWGWKDPRNTFTLPFWLDIFPDAKVIHIYRHGVDVAHSLRVSQEKGLSR
                     LRIKDSIYKSLYWYYLANKFIPNRKKFVYLRCSSLENGVSMWEEYLNEARSHVSNLGS
                     KAMEIKYEDLLQQPSVELKKLTAFCELEATDSDINRVSNLGNRSRAYAYKNSPELQEF
                     ADRSDIKKRLESVGY"
     gene            1754..>2151
                     /locus_tag="AC159U_01970"
     CDS             1754..>2151
                     /locus_tag="AC159U_01970"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF024912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR3335856.1"
                     /translation="MCGIVGFCDCNNKADRGVLISMRDALTHRGPDDKGEYIDEKNNI
                     GLAHARLSILDVSSLGRQPMSNDKGTVWVTYNGEIYNFKEIRQDLIQHGYSFKSNSDT
                     EVLIKAYEQWGIDCIHKFIGMFAIALWDSR"
BASE COUNT          648 a          370 c          403 g          730 t
ORIGIN      
        1 aagaagcatc atgaaaagaa cttttccagg aatcgagaag atctatgact tctttacaac
       61 tattataggc tattaccccg ccattgtatt gtggaaagca tagggggata tcctgagtcc
      121 agctaacagt tgttttaggc gagtttcttt tgtgagcatg ggccaaggca atgtcgaatc
      181 tttctaatag ttcaaagatg tcatatatcg aatcacagat tttagtgtcc gagtctaaat
      241 aaagcgtttt actaaaagga gtgtgtgtta aataatctac tttagatctg taatgcggat
      301 tatttattat cccaaatgaa gaaaaaatgt cgccatattc ttttactagt tctgtttgtt
      361 ttttatccac gaagatatga atattgagct taggagaata ttttttaacc gtaatggctg
      421 aattgatagc cgcctttgta taggttttac cagatgcaat gtataaaaca ccatcgccaa
      481 tccgtttaac gttttctttc atttattata tctatttaca catttaaatt ttatttagag
      541 ataatgtttt agtatataat ctgaattata ccacatgagc tttggcctaa actaaataaa
      601 ccgtttatat attcaccaat taggattgca ggtttgtgtt taaaggaatt gcagtcaata
      661 tcctacggat tccaatcttt ttttaatatc actgcggtct gcaaattctt gcagttcagg
      721 actgttttta tatgcataag cacgggacct gttacccaga ttagagaccc tgtttatatc
      781 actatctgtt gcttctagtt cacagaacgc ggttaatttt tttaattcta cggatggttg
      841 ctgtagaaga tcctcgtatt ttatttccat agccttactt cccagattgc ttacgtgact
      901 cctggcctcg tttaaatatt cctcccacat agatactcca ttttcaaggg aagagcatcg
      961 caggtaaaca aatttctttc tgttagggat gaatttgttt gccagataat accagtataa
     1021 acttttatat atagagtctt ttattctcag gcgcgagagt cctttctctt gactaacccg
     1081 taagctgtgt gctacatcaa ccccgtgtct gtatatatga attacctttg catccgggaa
     1141 aatatccagc cagaagggca gcgtaaaagt gttgcgaggg tccttccatc cccaggggat
     1201 atcaaggttt gccggggttc tatgagataa atatttttta aatcccagat aagaaatagc
     1261 cttcggagtt ttcattgtgt gattaataaa ttctgcaaat agtgttctta cttccttgtc
     1321 ttctaaaatg tatttaatag actgtggatt ttccagcccg ccgcttgatt gttcgagcag
     1381 ccacatgtta aggtcgataa agaaaagggc ctcgtggttc ctgtccttgg attttcctat
     1441 aaaaagccca aggtcttcga gcatctttgc aaggattccc gtaccagacc tgctcattcc
     1501 aattataatt gtgggtgggt gtgctgacat tgctttttta aataagagta aagtttatat
     1561 aataataagg aataatctat agtaaacgat ataatttaat aatttaataa tttaatggtt
     1621 taaaagactt aaatcttacc tatcactttt ttgtttatta tttaatttgc tgttctaatt
     1681 agatgtagtt agtaataatt gaaaataaca tttcttttct ttaccattaa aactttttat
     1741 aggacaatat atcatgtgcg ggatagtagg attttgcgac tgtaataata aagccgatag
     1801 gggtgtatta atctccatga gagatgcact cactcataga gggcctgatg ataagggaga
     1861 atatattgat gaaaaaaaca acatcgggct tgcacacgca aggctttcta tactcgatgt
     1921 ttcttctctt ggcagacagc ccatgtctaa tgataaaggc acagtatggg ttacatacaa
     1981 cggagagatt tataatttta aagaaataag gcaagatcta atacagcacg gctacagctt
     2041 taaaagcaac tcagacaccg aagttttaat aaaagcttat gagcagtggg gaattgactg
     2101 tatacacaag tttataggaa tgtttgcaat tgccctgtgg gactcaagag a
//
LOCUS       DBNGSV010000054         8318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1673195, whole genome shotgun sequence.
ACCESSION   DBNGSV010000054 DBNGSV010000000
VERSION     DBNGSV010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1673195"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..328
                     /locus_tag="AC159U_01975"
     CDS             8..328
                     /locus_tag="AC159U_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012153549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3335857.1"
                     /translation="MSEEYSESSFWTKIKNYAKSAGKEVIEKSLILYFCLKDPDTPSW
                     AKSVIAGTLGYFILPMDSIPDLTPFIGFSDDLGALAAAIGIVGIHIKKKHREMAKEKM
                     NEWF"
     gene            350..850
                     /locus_tag="AC159U_01980"
     CDS             350..850
                     /locus_tag="AC159U_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335858.1"
                     /translation="MISNELRETIDTQFRIKNLLWIGLTVGLILYFFAVYFITTGKEP
                     NPNISQMTQYVFAGFGFLLIIIAIAIRKFSFSEDRLKSVCLSAQINSDDWTEKITKYL
                     SRQQVFYIISLAINDSIGILGIAIGFISRDINKALPYILVAILLNIWVYPKKERFLAN
                     LQKTGQ"
     gene            complement(863..1546)
                     /locus_tag="AC159U_01985"
     CDS             complement(863..1546)
                     /locus_tag="AC159U_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR3335859.1"
                     /translation="MGVICHLISALLLFRAGELFDGSFDNSIFIFAFFIIVVFLIYHI
                     KSNTYVLGALVLPLVFLITLPSVVFPTDLVNAGDPGENPVLLIHIFITFLSQAIFTLA
                     FFAGLLYIFQQNRIKSKNISGLLKKFPSLSTLDSINHFCLLIGFPLLTIGLALGIIFT
                     KSKWDVFLRLQQKEIWAIITWFLYAFLIYGRLGIGWKGRKAAIGAIAGFIVIVITFIA
                     LGYLSSNPG"
     gene            complement(1721..2779)
                     /gene="prfA"
                     /locus_tag="AC159U_01990"
     CDS             complement(1721..2779)
                     /gene="prfA"
                     /locus_tag="AC159U_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009661037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3335860.1"
                     /translation="MIKQLEEVEKKCSDLDRQLSNPEISPTDIHKFSSERSKLTEVVE
                     AYREYKSVLEQIENNKELLKDKELQELAKEELEELEPRQQKLEKELQILLLPKDPNDE
                     KSIFIEIRAGTGGDEAALFAANLFRMYTHFAEKNGWKVEIMNINDTGIGGIKEVVASI
                     EGKNVYKKFKFESGVHRVQRVPATETGGRIHTSTATVMILVEPEDVEVDIDEKDLRVD
                     TYRASGPGGQHVNKTDSAIRITHMPTGVVAQCQDERSQHKNRAKAMRMLKAKIYELEE
                     EKRQSEISNIRKNMVGSGDRSEKIRTYNYKDVRVTDHRIGLTLHKLEQILEGELDELI
                     DALNDYQQNESLKQSLQN"
     gene            2860..3069
                     /locus_tag="AC159U_01995"
     CDS             2860..3069
                     /locus_tag="AC159U_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335861.1"
                     /translation="MKTIDVLESKIKEALEVKTKLEEKQQVLMEEIEQLRGSVEQLEI
                     EREKTKQTIDMIIEKVELYIKRAEA"
     gene            3072..3452
                     /locus_tag="AC159U_02000"
     CDS             3072..3452
                     /locus_tag="AC159U_02000"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3335862.1"
                     /translation="MKSIKIKIYNREYNIKTDADEEYIHSLASFLEQRLKENTSQVID
                     LNIPYPLLLAVFQIADDFFRTKTELEEFKKRAEDRSKQLVRILDDALKNGDTYAANTD
                     GTGQVSKITEPTASSNTKQEELFK"
     gene            3376..4062
                     /locus_tag="AC159U_02005"
     CDS             3376..4062
                     /locus_tag="AC159U_02005"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR3335863.1"
                     /translation="MEQDKLVKLQNPQQVQTPSKKNYLNNSSDSCINDYGSNEIKNSL
                     RIHYLQKRLSLCKKDLKYKSAVISKNLINHPVYQNSEIIALYYPIRSEVDVLNIFDTN
                     RKLGKKSCFPKVCGNTLRFYEVSEKSHLKSGTYGILEPNNRDCKEISPEDIDLFIIPA
                     VCIDYRGNRIGSGKGYYDRALANIDSSKKLGLVFENQITCTIPADEFDKHVGYIATEA
                     GVIKIRRRGN"
     gene            4064..5620
                     /gene="rny"
                     /locus_tag="AC159U_02010"
     CDS             4064..5620
                     /gene="rny"
                     /locus_tag="AC159U_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3335864.1"
                     /translation="MDIQSIAFLIAGLIVGAAIIFIYKASLEKKDAENARSEAEKITD
                     GAKNEAEKIRLEAELKAKQIVDKSKSEADKLTREQQKEISRIEKKLQNKEDNLDKKYE
                     NLERRANDLTRKEQELPQREKELEDKQAELDAIIEEAKSKIEQIAGMSQEAAKRELVN
                     VIENEARHEAAKKLKIIEDELNETASKKARDVLALAAQRYAGEYASEKTVSVVQLPSD
                     DLKGRIIGREGRNIRSLELRTGVDLIIDDTPETVVISSLNPIRREVARKSLEYLIQDG
                     RIHPGRIEEIVSQVEEELDKEMQKSGEEAVFDLGIHGMHPELIKLIGKLKYRTSYSQN
                     MLTHSIEVAFICGIIASEIGFDQKAAKRAALLHDIGKAVDHEQEGSHVEIGEELVRKY
                     GERADIIEAVATYHDPSPTSMLAVLVQAADAVSAARPGARRETYEAYIKRIDDIERIA
                     GSFRGVDKCYAISAGREVRVLVESDMVNDEESVLLSSEIAQKIEKEVTYPGQVKITVI
                     REVRSSAQAS"
     gene            5697..5770
                     /locus_tag="AC159U_02015"
     tRNA            5697..5770
                     /locus_tag="AC159U_02015"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5731..5733,aa:Ile,seq:cat)
     gene            5938..6672
                     /locus_tag="AC159U_02020"
     CDS             5938..6672
                     /locus_tag="AC159U_02020"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3335865.1"
                     /translation="MKEQLAALEKLQQIDSELMGMEVDLNKYPKEISSLQNELEKDKQ
                     DISNLEQQLQTIQSDKTQMEQTLEQNNQNIKSTEDRLFEIKTHKEYVALQKEIAESKR
                     ANSDIEENILKEMERIEQIEEKLAKLKEDYSVKETEYTERINDQKNKLSELQSAYDPI
                     KAKKDQQSSEIQKEYLQRYERARNKNGSAMALAENERCTGCHMNIPAQLFNEVLKLTK
                     MIQCPNCKRILYTQDVLESEEVSEDN"
     gene            6669..7103
                     /locus_tag="AC159U_02025"
     CDS             6669..7103
                     /locus_tag="AC159U_02025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HIR3335866.1"
                     /translation="MNAHKNSDYYSIFTDGASRGNPGKSGIGIVIKDQSNNTIKVIKK
                     YFPKLTNNQAEYKALLLSLDYLTSNDIDLPVKFFTDSQLLANQINGNWKVKHPDIAVL
                     FKEAKALISKLSSFEISYIPREKNKEADRLANEAIDEYSIYE"
     gene            7097..7448
                     /gene="rnpB"
                     /locus_tag="AC159U_02030"
     ncRNA           7097..7448
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC159U_02030"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7442..>8318)
                     /gene="tyrS"
                     /locus_tag="AC159U_02035"
     CDS             complement(7442..>8318)
                     /gene="tyrS"
                     /locus_tag="AC159U_02035"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3335867.1"
                     /translation="ESQVFKIIDRFKTEIVFNSAWLGDLGAEGMLELSGLENVARMLE
                     RDDFEKRYKSGKPITIKEFMYPLMQAYDSVSIKSDVELGGTDQKFNLLLGRDLQRHYE
                     QAPQIVLTMPILEGLDGTRKMSKSLDNYIAVEDSPEDMYGKVMSVSDDLMWKYYDLLT
                     SVSDQETNNLKSGHPMEAKRNLASLIVKWLYDDGKASEAKSAFETKFSKREFPEEAKE
                     VSLSRNDISDLINFILEISDKIKSKSDARRLIQQGGLTINGEKISDIDSEIPAVDSLE
                     IKLGKREFVKVIIKN"
BASE COUNT         2894 a         1554 c         1571 g         2299 t
ORIGIN      
        1 agaggatatt tctgaagaat attcagaatc atctttttgg acaaagatca agaactacgc
       61 aaaatccgcc ggcaaagaag tcatagaaaa atcactgatt ttatatttct gcctcaaaga
      121 ccccgacact ccttcatggg caaaatctgt tattgccgga actctcggat atttcatact
      181 gcctatggat tccatcccgg atttaacccc atttataggc tttagtgacg atcttggggc
      241 gcttgcagcc gctattggca ttgtaggtat tcacattaag aaaaagcaca gagaaatggc
      301 aaaagagaaa atgaacgagt ggttttaaca agaatattga ttaaaagcta tgatatcaaa
      361 tgaattacga gaaactatag atacccagtt tcgcatcaaa aatctcttat ggatcgggct
      421 tactgtaggt cttatcctct atttttttgc tgtatatttt attactaccg gtaaagagcc
      481 taacccgaac atttctcaga tgactcaata tgtatttgcc ggatttggat ttttattaat
      541 tataatagct atagcaatcc gtaaattcag tttttctgaa gacagactaa aaagtgtttg
      601 tttatcagca cagataaatt cagacgattg gactgaaaaa attacaaaat acctatcaag
      661 acaacaggtg ttttatatca taagcctagc aataaatgac tccattggaa tactaggaat
      721 tgcaatagga tttataagca gagacatcaa taaagcactg ccatatattt tggtagcgat
      781 tttacttaat atttgggtat atccaaaaaa agaaaggttt cttgctaact tacaaaaaac
      841 agggcagtaa taattaataa ttttagccgg gatttgaaga caagtagcca agtgcgataa
      901 aggtaattac aattacaata aatcccgcaa tcgctccaat tgctgcttta cggcctttcc
      961 accctatacc aagcctgccg tatattaaaa atgcatagag aaaccaggta ataattgccc
     1021 aaatctcttt ttgctgcaag cgcaaaaata catcccattt gcttttggta aatattatcc
     1081 caagagcaag cccaattgtt aaaagcggaa aaccaattaa cagacaaaaa tgattaatgc
     1141 tatccagggt tgagagtgac gggaatttct ttagcaaccc acttatattc ttacttttta
     1201 tcctgttttg ttgaaatata tacagaagtc ccgcaaaaaa tgccagagta aatatagcct
     1261 gactcaaaaa tgtaataaaa atatgtatta gaagcacggg attttcacca ggatcaccgg
     1321 cgtttactaa atctgtagga aaaactaccg atggaagagt gataagaaat accagaggga
     1381 gcaccaaagc acccagcaca taagtattgg attttatatg gtagattaaa aaaacaacta
     1441 ttataaaaaa cgcaaatata aaaatagaat tatcaaaact gccgtcaaac aattccccgg
     1501 ctctaaaaag caataacgca cttattagat gacatattac acccacataa agtgaataat
     1561 tgactagaga tattagcaat ctggaattat tccaaagatt atatattaaa attatagaag
     1621 aaagaaaata tataagtaca gctatctgct gaaatatcat tataatatat taatacatta
     1681 taatagagta ttaaataaat tattttaaat aataagagat ttagttttga agactttgct
     1741 taagtgattc attttgctgg taatcattta atgcatcaat aagctcatca agctcccctt
     1801 cgagaatctg ttcaagttta tgcagggtaa gcccgattct gtgatctgta acgcggacat
     1861 ctttataatt ataagtcctt atcttttcac tcctgtctcc gcttcctacc atattttttc
     1921 tgatgtttga aatctctgac tgacgcttct cttcttcaag ctcataaatc ttagccttta
     1981 gcattctcat agccttggct ctgtttttat gctgagatct ctcatcctga cattgtgcaa
     2041 ctacacccgt gggcatatga gtaattctta ttgcagaatc tgttttattt acgtgctgac
     2101 caccagggcc cgaagcgcgg taagtatcaa ccctcagatc cttttcatct atatcaactt
     2161 ctacatcttc tggctcaaca agtatcatta cagtggccgt agaggtatgt atcctgcccc
     2221 ccgtttcagt ggcgggaacc ctttgcaccc tgtgtacccc gctctcaaat ttgaattttt
     2281 tatatacatt tttgccttcg attgatgcaa ccacttcttt aattccacca atgcctgtat
     2341 cattaatatt cattatctct actttccacc catttttttc tgcaaaatgc gtatacatac
     2401 gaaataaatt tgcagcaaat agtgcagctt catctccgcc tgtccctgcc cttatttcaa
     2461 taaaaatgct cttttcatca ttaggatctt ttggtaaaag cagtatctga agctcctttt
     2521 ccaatttctg ctgccttggc tccaattctt ccagttcttc tttagcaagt tcctgtaact
     2581 ctttgtcttt taacaattct ttgttgtttt caatctgctc taatacagat ttgtattccc
     2641 tgtaagcttc tactacttct gtgagcttcg atctttcact tgaaaactta tggatatccg
     2701 ttgggcttat ttcaggattt gaaagttgtc tgtcgaggtc agagcatttc ttttcgacct
     2761 cttcgagttg ttttattatt tctatattgc tcatttgttg gtataatgtt tccacatctt
     2821 gcaataatat caagatagga ttaaactgat tagaaagtta tgaaaacaat tgacgtcctt
     2881 gaaagcaaaa ttaaagaagc attggaagta aaaacaaagc tcgaagaaaa gcagcaagtg
     2941 ctaatggaag aaattgagca gcttagaggt agtgtagaac agcttgagat agaacgagag
     3001 aagactaaac aaacaattga tatgataatt gaaaaggttg agctgtatat taaaagggca
     3061 gaggcttaaa cttgaaaagt attaaaatca agatttacaa cagggaatac aatataaaga
     3121 ccgacgctga tgaagaatat attcacagtt tagcttcttt tttagaacaa cgattaaaag
     3181 aaaacacttc tcaggtaata gatttaaaca taccctaccc tttactgctg gcggtttttc
     3241 aaatcgcaga cgatttcttc cgcacaaaga cagaattaga ggagtttaaa aaacgtgcag
     3301 aagatagatc aaagcagctt gtccgaatcc tggacgatgc tctcaaaaac ggagatacat
     3361 acgcagccaa cacagatgga acaggacaag ttagtaaaat tacagaaccc acagcaagtt
     3421 caaacaccaa gcaagaagaa ctatttaaat aatagctctg atagctgcat taatgattat
     3481 ggttcaaatg agattaaaaa ctctttaagg attcactatt tacaaaaaag actaagtcta
     3541 tgtaaaaaag atcttaaata taaaagtgct gtaatatcta agaatttaat aaaccaccct
     3601 gtatatcaaa atagcgaaat cattgcactt tattacccta ttcgctccga agtcgatgtt
     3661 ttaaacatat ttgatacaaa cagaaagctt ggaaagaaat catgctttcc aaaagtttgt
     3721 ggtaacacat taaggttcta cgaagtatct gagaagtctc atctaaaatc aggaacttat
     3781 ggcattttag aacccaacaa tagagactgt aaagaaatat cccccgaaga tattgaccta
     3841 ttcatcatac cagctgtttg catcgactat agaggcaaca ggataggctc aggaaaggga
     3901 tactacgaca gagctttagc taatatagat tcatctaaaa aacttggttt ggtatttgaa
     3961 aatcaaatta cctgcacgat acccgctgat gaatttgata aacatgttgg gtatatagca
     4021 acagaggcgg gagtaataaa aattaggaga agaggtaact aatatggata ttcaatcaat
     4081 agcgtttcta atagctggac ttatcgttgg agcagcaatc atattcatct acaaagccag
     4141 tttggaaaag aaagacgctg aaaatgcaag gtcagaagca gaaaaaataa ctgatggagc
     4201 aaaaaatgaa gcagaaaaaa taaggcttga agcagagctt aaagctaagc agatagtgga
     4261 taaatcaaag tcagaagctg ataaattaac acgtgagcag caaaaagaga tttctaggat
     4321 tgaaaaaaag cttcaaaata aagaggataa cctggataaa aagtatgaaa acctagaaag
     4381 aagggcaaac gatcttacaa gaaaggaaca ggaactgcct caaagagaaa aggaattaga
     4441 agacaaacag gctgagctcg atgctattat tgaagaagca aaatccaaaa tagagcagat
     4501 tgcaggaatg tctcaggaag ccgccaaaag agagttagta aatgtaatag aaaacgaagc
     4561 tcgtcatgaa gctgccaaga agcttaaaat tattgaggat gaactaaatg aaacggcctc
     4621 aaaaaaagca agagatgtac ttgctctggc agcccagaga tatgcaggag agtatgcaag
     4681 cgaaaaaaca gtttcagtgg tgcagcttcc aagcgatgac ctaaagggaa gaataatcgg
     4741 aagagaggga aggaatatca ggtcactcga gcttagaacc ggcgtagatt taattattga
     4801 tgacacaccc gaaacagtag ttatatcatc acttaacccc attagaaggg aagtagccag
     4861 aaaatccctc gagtacttaa tccaggatgg aaggattcac ccgggaagaa ttgaagagat
     4921 agtaagccag gttgaagaag agcttgataa agagatgcag aaatccggtg aagaagcggt
     4981 atttgatctt ggcattcatg gtatgcaccc agagcttatt aaacttatag gtaaacttaa
     5041 atatagaaca agttactcgc aaaatatgct tacgcactcg atcgaggttg catttatatg
     5101 cggcataata gcctctgaaa tcggatttga tcaaaaagcg gctaaaagag ctgcactgct
     5161 gcatgatata ggaaaagccg tggatcacga acaggaaggc tctcatgtgg aaattggaga
     5221 agagcttgtt agaaagtacg gagaacgtgc agatataata gaggctgttg ccacatatca
     5281 tgatccaagc ccaacttcta tgcttgcagt gctggtacag gctgcggatg ccgtatcagc
     5341 cgcaaggccc ggagcaagaa gggaaactta tgaagcatat attaaacgca tagatgatat
     5401 agaaagaatt gcaggctcat ttagaggtgt agataaatgc tatgcaatct ctgctggcag
     5461 ggaagtaaga gtgcttgtgg aaagtgatat ggtaaacgat gaagaatcgg tgctactatc
     5521 tagtgaaatt gcacaaaaga ttgaaaaaga agttacgtat ccagggcagg ttaaaattac
     5581 ggtaatcagg gaagtgaggt catctgccca ggcttcataa tttataaata ctaaaaatat
     5641 aaattagtat tgaacagcaa attaaatctg ttataatgca tggttctgta ttattagggc
     5701 ccatagctca gtaggttaga gcggcggact cataatccgt tggtccctgg ttcgagtccg
     5761 ggtgggccca aattatttat attagcaatt tgtaatttgg aactaaaata gctataaaaa
     5821 tgggtaaaac caaaaagatc attaaaacta actcaagtaa ccatatattt ctaaccacat
     5881 ataagtaccc aattttatac cttaaagact tattctatat ttatttagga gggaagcatg
     5941 aaggaacagt tagcagctct tgaaaaactg cagcaaattg actcagagct gatggggatg
     6001 gaggttgacc ttaataaata cccaaaagaa atatcttcat tacaaaatga acttgaaaaa
     6061 gacaaacaag acatatcaaa cttagagcag cagttacaaa caatacagtc agataaaacc
     6121 cagatggagc agaccttaga gcagaacaat cagaatatta aatctaccga agacagactt
     6181 tttgagatta aaacccataa ggaatatgtt gctcttcaaa aagaaatagc tgagtcaaaa
     6241 agagcaaatt cggatataga agaaaatatc ctcaaagaaa tggaacgtat tgagcagatc
     6301 gaagaaaaat tagccaagct caaagaggac tacagtgtaa aagaaacaga atatacagaa
     6361 agaattaatg atcaaaagaa taaactcagc gagctacaat cagcttatga tcctattaag
     6421 gcaaaaaaag accagcagtc atccgaaata caaaaagagt atcttcaaag gtatgaaagg
     6481 gctcgaaaca aaaatggatc tgctatggcg cttgctgaaa atgagagatg cacaggctgt
     6541 cacatgaata tccctgccca gctatttaac gaagtgctca agcttacaaa aatgatacaa
     6601 tgtccaaatt gcaagagaat cctctatact caagatgtgc tagaatctga agaagtttct
     6661 gaagataatt gaacgctcat aaaaactctg attattatag catatttacc gatggcgcct
     6721 ctaggggaaa ccctggtaaa tcaggtattg gtatagtaat taaagaccag agtaataata
     6781 caataaaagt aatcaagaag tatttcccca aacttacaaa caatcaggct gagtataagg
     6841 cgctcttact atcacttgat tacttaacat caaatgacat agacctgcct gtaaagttct
     6901 tcacagactc gcagctcctt gcaaaccaga ttaacggcaa ttggaaagtt aaacaccctg
     6961 atatagccgt gctgttcaag gaagcgaaag cactaatttc caagctgtcc tcatttgaaa
     7021 tctcatatat accaagagag aaaaataaag aagctgacag gcttgcaaat gaggccatag
     7081 atgaatattc tatttatgag tgaaccatgc ggtcgctggt tttgatttaa tcaaaaccag
     7141 aggaaagtcc gaactccgca gggcatggcg ctgggtaacg cccaggtacc gtgaggtaac
     7201 ggaaagtgca acagaaaaca taccgccact ttttagtggt aagggtgaaa tggtggtgta
     7261 agagaccacc gcgcaaagtg gtgacacatt gcggcattgt aaaccccgcc aggtgcaaga
     7321 ccatgtagga agcgcccgaa gccggcccgg ctgatgcttc cgggtgggtc gcatgaggca
     7381 gtcagtaatg gctgtcccag atgaatgacc gtactagaca gaattcggct tacaggttca
     7441 ctcaattttt tattattact ttaacaaact ctctcttgcc cagtttaatt tccagtgaat
     7501 ctactgctgg aatttccgag tcaatatcac ttatcttttc tccatttata gtaagcccac
     7561 cctgctgtat aagccttctg gcatctgatt tagacttaat cttatcagat atttctaaaa
     7621 taaaattaat caaatccgaa atgtcatttc tactcagtga aacttctttt gcctcctctg
     7681 gaaactcccg ttttgaaaac ttggtctcaa aagcgctttt tgcttcagag gctttaccat
     7741 catcataaag ccatttaact atcaatgacg ccagatttct ctttgcctcc atagggtgac
     7801 ctgatttcaa attattagtt tcctgatcgc ttacagatgt taagagatcg tagtatttcc
     7861 acattaggtc atcagaaaca gacattacct ttccgtacat atcctcagga ctatcctcta
     7921 ctgctatata gttgtccagt gacttggaca tttttcttgt gccgtccaaa ccctcgagta
     7981 tcggcatggt aagaactatc tgaggagcct gctcgtagtg cctttgcagg tcccttccaa
     8041 gaaggagatt gaacttttga tctgttccgc caagctcaac atctgatttt atgctcacag
     8101 aatcataggc ctgcataagc gggtacataa actcttttat agtgatgggc tttcctgact
     8161 tatatctctt ttcaaaatcg tctctctcaa gcatccttgc tacattttca agccctgaga
     8221 gctcaagcat gccctctgcc cctaaatcac ccagccatgc agaattaaat acaatctcgg
     8281 ttttaaatct gtctatgatc ttaaatactt gtgattca
//
LOCUS       DBNGSV010000055        19422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_426370, whole genome shotgun sequence.
ACCESSION   DBNGSV010000055 DBNGSV010000000
VERSION     DBNGSV010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19422
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_426370"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="AC159U_02040"
     CDS             <1..118
                     /locus_tag="AC159U_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335868.1"
                     /translation="KTGSASRSDRMAKYNQLLRIEEELGSVAQFLGRRAFRV"
     gene            180..1598
                     /gene="leuC"
                     /locus_tag="AC159U_02045"
     CDS             180..1598
                     /gene="leuC"
                     /locus_tag="AC159U_02045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3335869.1"
                     /translation="MGKQTLFDKIWEKHVVYEEQGRPSLLYIDLHLVHEVTSPQAFEG
                     LRLSNRKVRRPELTFATMDHNVPTTDRSLPIEDPISAKQIETLRNNCEEFGIKLFDLN
                     SPFQGIVHVIGPELGITKPGMTIVCGDSHTSTHGAFGSLAFGIGTSEVEHVLATQCLW
                     QNRPSVFEVNVEGSRHNGVTAKDIILSIIGKIGTAGATGTVVEYRGQAIKDLSMEERM
                     TVCNMSIEAGARAGMISPDEKTFEYLKGRQYMPKDEEYDKCVEKWAELATEDGATFDK
                     SITLDASRIAPQVSWGTNPGMVVGVDEKVPGPADYSDPNLQKATQSALEYMDLKPGTK
                     IEDIKLDRIFIGSCTNSRISDLIAAAKVVKGKKVASSLSAMVVPGSQLVKLQAEKLGL
                     DKIFKESGFEWREAGCSMCLGMNPDILVPGERCASTSNRNFEGRQGKGGRTHLVSPIM
                     AAAAALKGHFVDVRDLDLETEL"
     gene            1610..2200
                     /gene="leuD"
                     /locus_tag="AC159U_02050"
     CDS             1610..2200
                     /gene="leuD"
                     /locus_tag="AC159U_02050"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3335870.1"
                     /translation="MKPFENFKGIVAPLDKMNVDTDQIIPKQFLKRIERTGFGEFLFY
                     DWRYLDDGKPNPDFILNKEGYKNSQILLAKDNFGSGSSREHAPWSLEDYGFKVIIAPS
                     FADIFYNNCFKNGMLPIILSSDLVDYLFKQATTQNGYMLDINLDDQIIKDDSGWSTTF
                     DIDPFKKKTLLEGLDDIALTLQLQDHISSFEQSHTV"
     gene            2421..3935
                     /gene="rpoN"
                     /locus_tag="AC159U_02055"
     CDS             2421..3935
                     /gene="rpoN"
                     /locus_tag="AC159U_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3335871.1"
                     /translation="MSAIELKQNTKLVQQQKLYLTQEQQLFLRLIQMSSLELKEYVEE
                     QLVENPVLEEDLEKKPKDDAQTDNNSSEDLNFDSYKFDTDNSIPFTNKNFVDQEQDYS
                     WENNLTSETSLQDYLRWQLKISNLGEEDKLIASIIIGNTDEDGYLNSKCDEIFAEFLN
                     ISSNTSINDKSANSLALNNITSEHLQQGIGKVENVLDELHSTFDPVGVCARDLKECLK
                     LQAYNLGFRDEDLIIILIEKYLEDISNNDFEKIAAEMQKEIDEIKEAVNMISSFEPKP
                     GRPYYMSDIEKNITPDYHVYRIGDELQIQFKVNIPNLRISQYYQRIVKNSDSINSETK
                     KYIKEKLDIAKKVIKGIQEREIAITKVIKKIVDVQRDYFFYGKDFIKPLKLKDIADDE
                     DINVHESTISRMTSKRYISTPNGVIPMRTLFSRKIETVHGTDISYDRVRSIIEDLIKN
                     EDKNSPYSDEDISKILEIRNIKLARRTVAKYRKLLDIPSSSKRAKNYKEQQNGS"
     gene            3925..4461
                     /locus_tag="AC159U_02060"
     CDS             3925..4461
                     /locus_tag="AC159U_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043024 - ribosomal small subunit binding
                     [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promotion factor"
                     /protein_id="HIR3335872.1"
                     /translation="MDLKITIRHEKDARKSRRLRDFIKKKSLKIEKIINQYNTPSEVK
                     IILDVQKFRNKAEIFINSGIFKCTSSVESEDMANSIESAIDNVIKQLKKRIEKKASLK
                     KRKGAEKVIDTHYVMGKTDQSDYRLENAPAKPMSVDEAKLQLDISEHEFVTFYNSSSG
                     EMNVLYKNSADELVLVTP"
     gene            4491..4952
                     /locus_tag="AC159U_02065"
     CDS             4491..4952
                     /locus_tag="AC159U_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3335873.1"
                     /translation="MNITDELTKDNIIADLQSTTKTDVINELTAHICKNHLNINHEQA
                     SEVIMEREKLCSTALDAGVAIPHGKIGGISSFAVVFGRSLKGIDFDSLDGKPTQLFIL
                     ILSPENSSGMHLKLLAKISKILKIPETRSRLINAGSSDEIYDIIAEEDAKY"
     gene            4939..5877
                     /gene="hprK"
                     /locus_tag="AC159U_02070"
     CDS             4939..5877
                     /gene="hprK"
                     /locus_tag="AC159U_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR3335874.1"
                     /translation="MQNTKILKQFGGKSKAKPGDKLTVGELLSSWGNELQLTLITGSK
                     GFKRTIKENRVQKIGLRMIEPDITLDKFTVQILGRTEISYLKKISASEQQQVLLKLTS
                     QKIPCLIVTKGIKPPENLVKQCEKAGIPLFRTNLHTGRTISEIRAILEVRFAPFISAH
                     GVLIDIHRLGVLVLGKSGIGKSECALDLVLRGAKLVVDDLVEIRRLGASNLVGCSPQN
                     IKHLMEIRGIGIINIKDLVGPTAVMDKREIDMVIELEHWDPKTEYNRVGDVYDTYEIL
                     DVKLPYLKIPVSPGRNISTIVEIAVRNQIFRQSVSD"
     gene            5904..6752
                     /gene="rapZ"
                     /locus_tag="AC159U_02075"
     CDS             5904..6752
                     /gene="rapZ"
                     /locus_tag="AC159U_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3335875.1"
                     /translation="MNLVIVSGLSGSGKSSAINVLEDLGYYCVDNLPPQLIHTFLNLC
                     KKSSAQIENVAFGIDIREGEFLNKIHEEIKKLKKSGKKVQSIFLDASDQSLIKRYKES
                     RRVHPLAPTGNLPEGIKNEREALREIKQSSDNVIDTTKLTVHQLKQIIIDIFGKSKKQ
                     KLILNFSSFGYKYGIPNDPDIMIDVRFLPNPHFNEKIKKFNGKDIKIKRYVLKGDEPK
                     VFLDKLVDFLDFLFDKYILEGKSYLTVAIGCTGGKHRSVVIVEELKKRFKKLNPNISH
                     RDIGKE"
     gene            6757..7161
                     /locus_tag="AC159U_02080"
     CDS             6757..7161
                     /locus_tag="AC159U_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3335876.1"
                     /translation="MIGIVVISHGKFAEELISAVNFVLSAKPKIKMNAINLDREVDFD
                     AFKKEIKRNIKKADKGDGVLLVTDMFGGTPSNISLTFLEENKVEVISGVNLPMLIKLA
                     TLPENTTLSESVRIAEKAGRDNIIVASNLIKK"
     gene            7184..7513
                     /locus_tag="AC159U_02085"
     CDS             7184..7513
                     /locus_tag="AC159U_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335877.1"
                     /translation="MRIEKSEEILDKWKDSILAGDLQDYTLKIDDDVKEDLGLIALFL
                     DYMTVKASGNAMDYYEGYKQAASDILGLTGLYLVQDDHNKLINVKYQAPDDDIQEQLK
                     KHIWGDE"
     gene            7532..8344
                     /locus_tag="AC159U_02090"
     CDS             7532..8344
                     /locus_tag="AC159U_02090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR3335878.1"
                     /translation="MQLADLFDSFLIDLDGVIYVGDSPTYKAAQVITKLTKAGKEVIF
                     ITNDPRRSSVGYCKKLKSIDINIPANYIVTSASALSRHIETKYNTKSKTAFVVGSDNL
                     KKEVQRTGLKLITKISKVNSDFVIVGGHNKVDYNDIKKATLCIRAGAKFFATNNDPYY
                     PTHEGLVPATGALVAAIETASSAKATVVGKPKRIMFDIAKNLLSSNKKVAVIGDRLDT
                     DIAGGKRAGCSTILSLTGSTKAEEIKSSKTKPDFVIRDLSFLYKDSEISAYK"
     gene            complement(8346..9113)
                     /locus_tag="AC159U_02095"
     CDS             complement(8346..9113)
                     /locus_tag="AC159U_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3335879.1"
                     /translation="MKLLKVFIVIIAILYLGVPNSAAEEFDHSHKNYDQLLQDQVKDG
                     LVNYKAIKVDPEVLNGYLDTLGKVEKATFNAWPEKERLTYLINLYNAATIKLIVDNYP
                     VKSIRDINKEGEGPWKLNVVNLFGKKISLDALEHDIIRKNYNEPRIHFALVCAALGCP
                     KLPNWAFVSLKLEQQLEARTKLYLADKSINYIDEGNKTIYLSSIFNWFAGDFKKTHST
                     VLDFVKQYLPADEANKLNPNFRVNYTEYDWNLNDSSR"
     gene            complement(9332..10729)
                     /locus_tag="AC159U_02100"
     CDS             complement(9332..10729)
                     /locus_tag="AC159U_02100"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3335880.1"
                     /translation="MAKSGYRTVSDSMGEMEVPKDAYYAAQTARAIDNFPVSGVGLPK
                     EFIKAMALIKLSAAEANMSLGLLPKNLGDAIIKAAKEVADGKLDSQFVVDIFQTGSGT
                     STNMNTNEVIATRANEIIGKSAKKIHPNDHINMGQSSNDSIPTAMHVSALTEIQNSLI
                     PSLKKLQKSLSKKARQFDKIVKIGRTHLQDATPIRLGQEFSGYASMIEHGIARLEKVK
                     DELSELPIGGTAVGTGINTPKRFGSLIARNLTKITKVKFREADNHFEAQASKDAVVQT
                     SATLKTIAVSMMKIANDIRWLGSGPRCGIGEIMLPAVQPGSSIMPGKVNPVIAESVTQ
                     VSAQVIGNDAAITIGGQSGNFELNVMMPMMANNLLESIRLLTNVSHIFADKCIDGIEA
                     DKKRCEEMIEKSLAMCTSLAPIIGYDNAAAIAKEAYATGKTVRDVALEKKVMPEKELA
                     RVLDPFTMTEPGVPK"
     gene            10887..11687
                     /locus_tag="AC159U_02105"
     CDS             10887..11687
                     /locus_tag="AC159U_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3335881.1"
                     /translation="MPRAKTGEIELNYEIDGSGEQTLVFINGITMDTNGWGYQAPFFS
                     QFFKVVRYDCRGQGLSDKPANPYSQEMHADDLKNLLDVLGVDKVHLIGLSNGGMIAQH
                     FALKYPDKLISLILVDTCCYVDKLLQLMIDTWIKATEIGGNEFRYDMSLPVIFSETFI
                     RNNEENLSQMKENNLIANSPEAIINLVKATADHDLSHRIQEIKCPTLIIAGSEDILIP
                     PKHSSILHEKIEGSEYITIENCGHVPSIEKPAEFNEIVLNFLTKVNSA"
     gene            complement(11698..12441)
                     /gene="rpoD"
                     /locus_tag="AC159U_02110"
                     /pseudo
     CDS             complement(11698..12441)
                     /gene="rpoD"
                     /locus_tag="AC159U_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(12931..13230)
                     /locus_tag="AC159U_02115"
                     /pseudo
     CDS             complement(12931..13230)
                     /locus_tag="AC159U_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012367.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            complement(13220..14989)
                     /gene="dnaG"
                     /locus_tag="AC159U_02120"
     CDS             complement(13220..14989)
                     /gene="dnaG"
                     /locus_tag="AC159U_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3335882.1"
                     /translation="MNQHSDIEIVQNIKDNLSIVDHIQKFISLKNSGRDYLGVCPFHD
                     DHKPSMRVSDDKGLFHCFACGTGGDIIGFHMKFNNLDFVSALKELAEIAGVELPSRSS
                     KTNAKPNNELLYKINNFTQKIYLKNLYQTQEGEKALDYLASRGLKKELIEQFGLGYSI
                     NSWNDLSDRMIGKNVPLNKATELGLVNKSSKSQSGYYDRFRGRIIFPIFDLNNKIAGF
                     GGRTLFDDDPKYLNSPESPLYNKSSILYGLNVARNDIRKENKVIIVEGYIDLLSLFQN
                     GIKNVVATLGTSLTSNHAKLLKRFCENMVIVYDGDKSGVSASLRALDVFLKHGITPSV
                     VVLPENEDPSSLVEKIGKDKFINLIENSNSLIDFYFDKISEDFNSGSRSRNVIIKELV
                     DKLSLYSDSISRSVYTKKAAELFGLREQEIYSLLNNKKTSEKSKQPGNASESTSSYEH
                     IFLKVLLHYPQYIEKIVDDDIVDMLEDSKVRQIVKICSESGFNNTASIINHLDDSQTQ
                     RIVSELLLSSEDIQNEIIAEQIFEQSYKRIKLSSIKKEQRKIKSQLNSTGNKEPDYEI
                     ELLKEYDNLVKMEKNLSEQVYGA"
     gene            complement(15198..15377)
                     /locus_tag="AC159U_02125"
     CDS             complement(15198..15377)
                     /locus_tag="AC159U_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335883.1"
                     /translation="MGLIDTVRDQRDSWIKRFEEQRQEYSDPEIDKLSDEEQERIIQR
                     NRENVGKFSFDKDDK"
     gene            complement(15400..16713)
                     /locus_tag="AC159U_02130"
     CDS             complement(15400..16713)
                     /locus_tag="AC159U_02130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HIR3335884.1"
                     /translation="MARSKKRPAPKRKRVNRARKNKGTRVILATVSFIVLVAITLAAL
                     NLINSGISRDESRVRITEDELSSEIKKIDSRLNEIFSEIGLKKAEIISKNNYKRNKDD
                     INWDYKEVSIKTDNTDKFNKFNKKFASLTGLKNITTTVKKNKNSTNSSLDIFTLSTHK
                     INFHLEIPKPVPAKVPSKPEITKKEISPTAVPNRSKTKSAADGKEFAFLYGLKTKPKI
                     AIIVDDIGASKKSIDDLLKIPASINMAVLPDLKYSHYAASRANSKGWDVLLHMPMEPK
                     YASGYTGIDAGENALLTGLPKKEIMKLVEKNLAAVPHIKGVNNHMGSKFTESDELMNL
                     VLKKVKKEGLFFIDSKTSPQSRGYDEAKKLGLKTAERDVFLDKGKEGERFIRARVNEL
                     LRISKKQGYAVGICHPYPQTIRVLSDMIPKLKNYVEFIPASQIVN"
     gene            complement(16749..17876)
                     /locus_tag="AC159U_02135"
     CDS             complement(16749..17876)
                     /locus_tag="AC159U_02135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3335885.1"
                     /translation="MKKILLLLLMLVYVPDVYGDDEIYEGLSTFTRVLDLVERNYVDD
                     INSAELSVNAIEGMLQSLDPYSAYLTPDRFKDLEEGTSGEFGGLGMEITVKDGVLTVV
                     SPLEDTPASAAGIEAGDQIIEINGKSTSKMVEFEAVKLLRGPVGTSVNIKVKKKADSS
                     TKVIELERSIIYIKSVKSELLDRNLGYVKLIQFQDKTSGELKNAIKKLQQENNDKLQG
                     LILDLRNNPGGLLTQAVEVVDEFIDEGIIVSVKGRNSNQNLDYYATKGAETHKFPIVI
                     LVNNGSASASEVVAEALQDYERSTIVGTKTFGKGSVQTIIRLEDGSGLKITTAKFYAP
                     SGRSINNVGVVPDIIVENKDAKSDLQLKRATELIASKTSAN"
     gene            complement(17974..18951)
                     /locus_tag="AC159U_02140"
     CDS             complement(17974..18951)
                     /locus_tag="AC159U_02140"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR3335886.1"
                     /translation="MSKSEYSDYVLEQPGESIYIREVYGKTLVELGASHPDIVVLDAD
                     LTVSTKTNLFGKEYPDRFFTMGVSEQDMMATAAGFALAGKRPFASTFAVFATGRAWEH
                     VRQSIAFPNLNVKIVATHAGVTVGEDGATHQALEDICLMRVLPNMRVIVPADAVETEQ
                     VIRTMADNEGPAYVRLARAKFPTIYNDKYKFELGKASILRHGNDVGIFAAGIMVSEAL
                     NAAKLLEEKGIDACVVNLATIKPIDIETVVSVAEKTGAVVTAEEHNIIGGLGGAVSEV
                     LSEHCPTLVKRIGMKDRFGTSGNGMKLLEYFGLKAANISESCEQLLKSK"
     gene            complement(18948..>19422)
                     /locus_tag="AC159U_02145"
     CDS             complement(18948..>19422)
                     /locus_tag="AC159U_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HIR3335887.1"
                     /translation="LGHGLSASNGIALAGKLDNKDYRVYTLLSDGELQEGSTWEAITT
                     SGYRKLDNLCAIVDRNRFQNDGAMDKIKDINPVADKFIAFGWETEELKDGHDIESLIK
                     AFDRAKSTKGKPYAIIANTIKGKGVSIFENKGNYHGVAPSDEELKIALKELGEAA"
BASE COUNT         6181 a         3679 c         3471 g         6091 t
ORIGIN      
        1 taaaacaggt tcagcatcca gaagcgacag gatggcaaag tataatcagc tactaagaat
       61 agaagaagaa cttggaagtg tagctcagtt tcttggacga agagcattta gagtttaaaa
      121 ctctcaaaga gtgattatta taaaatatgt aaaattatgg gactataaaa taaaatacta
      181 tgggaaaaca aacactattt gataaaattt gggaaaaaca cgttgtatat gaagagcagg
      241 gaagaccatc gcttttatat attgatcttc acctcgtaca cgaagtaaca tctccacagg
      301 catttgaagg ccttaggctc agcaacagaa aagtccgcag acctgaactt acgtttgcca
      361 caatggatca caatgtacct acaacagaca ggtcgcttcc tattgaagat ccaatctcag
      421 caaaacagat tgaaaccttg agaaacaact gtgaagaatt tggaataaag ctttttgatc
      481 ttaacagccc ctttcaggga atcgtacacg taataggacc agagcttgga atcacaaaac
      541 ctggcatgac aatcgtatgt ggagacagcc atacttccac acacggcgct ttcgggtcgc
      601 ttgcttttgg aattggcaca agtgaagtgg agcatgtgct tgctactcag tgcctgtggc
      661 agaacagacc atcagtgttt gaggtaaatg ttgagggatc cagacataat ggtgttacgg
      721 caaaagacat aattctcagc attattggta aaattggaac ggccggggct acagggacag
      781 tggttgagta cagagggcag gcaataaaag accttagcat ggaagaaaga atgacagtgt
      841 gcaacatgtc aattgaagct ggggcaagag cagggatgat ttcacctgat gagaaaacgt
      901 ttgaatacct aaaaggcaga caatatatgc caaaggatga ggagtatgac aaatgcgttg
      961 agaagtgggc agagcttgct acagaagatg gtgcaacatt tgataagtcc attacacttg
     1021 atgcttcaag gattgctccg caggtaagct ggggtacaaa cccggggatg gttgtaggag
     1081 ttgatgaaaa ggttcccggt cctgctgatt attccgatcc aaacctacag aaggccacac
     1141 aaagcgcctt ggagtatatg gatcttaagc ccgggaccaa gatagaagat attaaacttg
     1201 acagaatatt cataggatca tgcactaatt caagaataag cgatcttata gcggcagcta
     1261 aggttgttaa gggtaaaaaa gtagcatcca gcctcagcgc aatggtagtg cctggctcgc
     1321 agttagtaaa acttcaggct gaaaaacttg gtcttgataa aatttttaaa gaatcgggtt
     1381 ttgaatggag agaagccggg tgcagcatgt gccttggaat gaaccctgat atactcgtac
     1441 ccggagaaag gtgtgcaagt acatcaaaca ggaacttcga aggaagacag ggcaaaggtg
     1501 gaaggactca tctcgttagc cccatcatgg cagccgcagc cgcacttaaa ggacattttg
     1561 tagatgtaag agacttagat ctggaaactg aattataaga ggtgaaaaca tgaaaccatt
     1621 cgaaaatttt aagggtatag tagccccgct agataaaatg aacgttgata cggaccaaat
     1681 tataccaaaa cagtttttaa aaaggattga gaggaccggt tttggagaat ttctatttta
     1741 tgactggaga tatttggacg acggaaagcc taatccggat tttatcctga acaaagaagg
     1801 gtataagaac agtcaaatcc ttcttgcaaa ggataatttt ggaagcggaa gctcaagaga
     1861 acacgcccca tggtcattag aggattatgg ctttaaagta attatagccc catcatttgc
     1921 cgatattttt tataacaact gttttaaaaa cggcatgctt ccaattattt tgtcttctga
     1981 tctggttgac tatctattta aacaggcaac tactcaaaac ggctacatgc tcgatataaa
     2041 tctagatgat cagataataa aagacgatag cggttggtct acaacatttg atattgatcc
     2101 gtttaagaaa aaaacattac tggaaggact ggatgatatt gcgttaactc ttcagttaca
     2161 agaccatatt tccagtttcg aacaaagtca tacagtatag atcgatataa tataattatt
     2221 gatttaaata agttatactt aatatttaaa gcattatcat aattattgtt aattaaatga
     2281 tttttcttga taaaatcatt taataacgtt cgaaaagata taccaatgca gcatttttca
     2341 atttctttga tatagaacaa aaaattagaa aaaatcaaga ctagtattta tccccaataa
     2401 gaattaatct ataagtaggt atgagtgcaa ttgagttaaa acaaaatacg aagctggttc
     2461 agcagcaaaa gctgtacctc acccaggaac agcagctgtt cttaagacta atacagatgt
     2521 cttcacttga actcaaggaa tacgttgagg aacagcttgt agaaaatcct gtgctggaag
     2581 aagatttaga aaaaaagcca aaggatgacg ctcagacaga taataacagt agtgaagatt
     2641 tgaattttga cagctataaa tttgataccg acaactctat tccttttacc aataaaaact
     2701 tcgtcgatca ggaacaggat tattcatggg aaaacaacct gacatcagaa acttccttac
     2761 aggattattt gcgctggcag ctaaaaatat ccaacctggg ggaagaagat aaactcatag
     2821 catcaataat cataggaaac accgatgaag acggttattt aaactcaaag tgcgatgaga
     2881 tttttgcaga attcttgaat atttccagca ataccagcat caatgataaa tcagctaaca
     2941 gcttagctct aaataatata accagcgaac acctgcaaca aggaataggt aaggttgaaa
     3001 atgttttaga tgaattacac tccacttttg accctgttgg ggtttgcgca agggatttaa
     3061 aagaatgtct caaacttcag gcatataacc ttgggtttag agatgaagac cttattataa
     3121 tacttattga gaagtatctt gaagacatta gcaacaatga ttttgaaaaa atagcagccg
     3181 aaatgcaaaa ggagattgac gagataaaag aggctgtaaa tatgatatct tcgtttgagc
     3241 ctaaacccgg aaggccatac tatatgagtg atatagaaaa aaatatcacg cccgattacc
     3301 acgtatacag gattggtgat gaactgcaga ttcagtttaa ggtaaacatt ccgaatttga
     3361 gaataagcca gtactatcaa agaatcgtta agaactccga ttctattaat tcagaaacca
     3421 aaaaatatat taaagaaaag cttgatatag ccaaaaaagt aataaaagga attcaggaaa
     3481 gagaaatagc cataacaaag gttataaaga aaatagtaga tgttcaaagg gattactttt
     3541 tttacggtaa agattttatt aaaccactta agctaaaaga tattgctgat gatgaagata
     3601 taaatgtgca tgaatcaacc ataagcagaa tgacaagtaa aagatatata tctactccaa
     3661 atggggttat tcccatgaga actctttttt ccagaaaaat tgaaacggta catggtacag
     3721 atatttcata tgacagagtt agatcaataa ttgaagacct tataaaaaat gaagacaaaa
     3781 acagccccta ttctgatgag gatatttcaa agatacttga aataagaaat attaaacttg
     3841 ccagaaggac tgttgcaaag tatagaaaac tgttggatat accttcatct tctaaaaggg
     3901 caaaaaacta taaggagcag caaaatggat cttaaaatca caataaggca tgaaaaagac
     3961 gcaagaaaaa gcagaagact aagagatttt ataaaaaaga aatctcttaa aattgaaaaa
     4021 atcataaatc agtacaacac tccttcagaa gtaaaaataa tcctggatgt acagaagttt
     4081 agaaacaagg cagaaatttt cattaatagt ggaatattca agtgtacctc ttcagttgaa
     4141 tcagaggata tggcaaactc catagaatct gcaattgaca atgtaataaa acaattgaaa
     4201 aaaaggatcg agaagaaggc ttcactgaaa aaaagaaaag gggcagaaaa agttattgat
     4261 acccattatg taatgggaaa aactgaccag agtgattaca gattagaaaa cgctcctgca
     4321 aaacccatgt ctgtggatga agcaaaactg cagctcgaca tatcagaaca tgaatttgtt
     4381 acattttata acagctccag cggggaaatg aatgttcttt ataaaaacag cgctgatgag
     4441 cttgtacttg taacaccata atactagttt acaatcctca ttgaattttc atgaacatca
     4501 cagacgaact tacaaaagat aatataattg cagacctgca atcgactacc aaaaccgatg
     4561 taataaacga actcacagca cacatatgta aaaatcatct caacataaac catgagcagg
     4621 caagtgaagt tataatggaa agagaaaaac tttgcagcac tgcgctcgat gccggagttg
     4681 ctataccaca cggcaagata ggtggtatat caagttttgc agtagttttt ggcagaagcc
     4741 tgaagggaat agattttgat tctcttgacg gaaagcccac tcaactgttt atactaatat
     4801 taagccccga aaattcatct ggcatgcatc ttaagcttct tgctaaaata tcaaaaatat
     4861 taaagatccc ggaaaccaga tctaggctca taaatgcagg ctccagtgac gagatatatg
     4921 atataattgc tgaggaagat gcaaaatact aaaatcctaa aacaattcgg aggcaaatct
     4981 aaagcaaaac ccggggataa acttacagtt ggtgaactct taagctcctg gggaaatgaa
     5041 ctacagctaa ctctaattac cggaagtaag ggatttaagc gtactattaa agaaaacagg
     5101 gtgcaaaaaa tagggctaag aatgatagag cccgacatta cgctcgataa attcactgtc
     5161 cagattctcg gaagaaccga gatttcatat cttaagaaaa taagcgcttc agaacaacag
     5221 caagttttat taaaactcac gtcacaaaaa atcccctgcc ttatagtaac caaagggata
     5281 aagccacctg aaaatcttgt aaaacagtgc gaaaaagctg gtattccact gttcaggact
     5341 aatctgcaca caggaaggac gatctctgaa ataagggcaa ttctggaagt acggtttgcc
     5401 ccatttatat cagcccatgg ggttttgatc gatattcaca gactgggagt gcttgttctt
     5461 ggcaaaagcg ggataggaaa aagcgagtgt gcccttgatt tagtcctacg gggagcaaag
     5521 cttgtggttg atgacctggt ggaaatcaga agacttgggg cttcaaatct tgtagggtgc
     5581 agccctcaga acattaaaca tttaatggaa ataagaggaa tcgggataat caacatcaaa
     5641 gaccttgtgg ggcctactgc tgttatggac aaaagagaga tagatatggt gattgagctt
     5701 gaacactggg atccaaaaac tgaatacaac agggttggtg atgtatatga cacttatgag
     5761 attttagacg taaaacttcc ctacctcaaa atacctgtaa gccccggcag gaatatatcc
     5821 actatagtcg aaattgcagt cagaaatcag atatttaggc aatctgtttc tgattagccg
     5881 tattattaga taatatataa tacatgaatc tagtaatcgt aagcgggctt tcaggatcgg
     5941 gcaaatcttc agctataaat gtacttgagg accttggtta ttattgtgta gacaatctgc
     6001 cgcctcagtt aattcatacc tttcttaatc tatgcaagaa atcatctgca cagattgaaa
     6061 atgtagcatt tggaatagac attagagaag gtgaattttt aaataaaata catgaagaaa
     6121 taaagaagct taaaaagagc ggcaaaaaag tccagtctat ttttttagat gcctctgatc
     6181 agagtttaat taaaaggtat aaagaaagca ggagggttca cccccttgca cccaccggaa
     6241 atcttcctga aggtataaaa aatgaaagag aagctctgcg agagattaaa cagagctcag
     6301 ataatgtaat agatactacg aaattaacag tacatcagct aaaacagatt atcattgata
     6361 tatttggtaa atctaaaaag caaaagctta tattaaattt cagctctttt ggatataaat
     6421 atggaatccc caacgatcct gacattatga tagacgtaag gtttcttcca aacccgcact
     6481 tcaatgaaaa gataaaaaaa tttaacggaa aggatataaa gattaaaaga tatgtgctta
     6541 aaggagatga acccaaggta tttttagata aacttgtaga ttttctggat tttctatttg
     6601 ataaatatat tcttgagggt aaaagttatt taactgttgc aataggatgc actggaggca
     6661 aacacaggtc ggttgtaata gttgaagaac ttaagaaaag atttaaaaaa ttaaatccta
     6721 atatatctca tagggacata ggaaaagaat aatatcatga taggtatagt tgtaatatca
     6781 cacggcaaat ttgctgaaga acttatatcc gcagttaact ttgtgctctc agcaaaacca
     6841 aaaattaaga tgaatgctat caatttagat agagaagtag actttgacgc atttaaaaaa
     6901 gaaattaaac ggaacataaa aaaagccgat aaaggagacg gagtactgct tgtaacagat
     6961 atgtttggcg gcacgccatc taatatcagc ctcacttttt tagaggaaaa taaagtagag
     7021 gtcatctcgg gggtaaatct cccaatgctt attaaacttg ccacactgcc tgaaaacact
     7081 acgctttcgg aatcagtaag gattgcagaa aaagcagggc gcgataatat tatcgtggct
     7141 agcaacctga taaaaaagta aataatatga taaggggcat aatatgagaa tcgaaaaatc
     7201 tgaagaaata ttagataaat ggaaagacag tatactggct ggtgacctgc aagattatac
     7261 attaaaaata gatgacgatg taaaagaaga tttaggtctc attgcgcttt ttttagacta
     7321 tatgactgta aaggcctcag gaaatgctat ggactattat gagggctaca aacaggcagc
     7381 ttctgacatc ctgggcttaa cgggcttgta cctggtgcag gatgatcata acaagcttat
     7441 taatgttaaa taccaggcac cggatgatga cattcaggaa caactgaaaa aacacatctg
     7501 gggcgatgaa taaagtttaa taagcttagt tatgcaactt gccgatttat tcgactcctt
     7561 tttaattgat ctggatggag tgatatatgt tggagacagc cccacttata aagcagcaca
     7621 agttattaca aaacttacca aagctggaaa agaagtaatc tttataacaa acgacccgag
     7681 aagatcatct gtggggtatt gcaaaaaact taaaagtatc gatataaaca tccctgcaaa
     7741 ctatatagtc acatctgcat ctgcactctc caggcatatc gagaccaaat ataatactaa
     7801 atcaaaaaca gcctttgtcg ttggttcaga taatcttaaa aaagaagtac aaagaaccgg
     7861 gcttaaactt attacaaaaa tttcaaaagt taactcggat tttgtaatag tcggcgggca
     7921 caataaggtt gactataatg atataaaaaa ggccacgctt tgcataagag ccggtgcaaa
     7981 attttttgca accaataatg atccgtatta tccaacacat gaaggtcttg tacctgcaac
     8041 aggtgcgctt gtggctgcaa tagaaactgc ctcatctgct aaggcaactg tagtaggaaa
     8101 accaaaaagg ataatgtttg atatagctaa gaatttgttg tccagcaata aaaaggtagc
     8161 cgttattggt gacaggctgg atactgatat tgcaggaggg aaaagagccg gttgttctac
     8221 aattttatca ctcaccggat caacaaaggc agaagagatt aaatcttcga aaactaagcc
     8281 tgactttgta ataagagatc tgagttttct atataaagac tctgaaattt ctgcatacaa
     8341 atagtctacc tactgctgtc attaaggttc cagtcatact cggtataatt cactctaaag
     8401 tttggattga gcttattagc ttcatcggca ggtaaatact gttttacaaa atccagaacc
     8461 gtgctgtgag tttttttaaa gtcacctgca aaccagttga atattgaaga tagatatatt
     8521 gttttattac cttcatcaat gtaattaata ctcttatctg ccaaatatag tttagttcgg
     8581 gcttctaact gctgttcaag ttttaatgaa acgaatgccc agttaggaag ctttggacac
     8641 ccaagagctg cacatactag ggcaaagtga attctcggtt cgttataatt ttttctgatt
     8701 atatcgtgtt ccagcgcatc gagagaaatc tttttaccaa aaaggtttac tacattcaat
     8761 ttccaggggc cctccccttc tttatttata tctcttatgc tcttaacagg ataattatcg
     8821 actataagtt ttattgttgc tgcgttatat aagtttatta agtaagtcag tctctccttt
     8881 tccggccatg cattaaatgt ggctttttca acttttccta gagtgtccag gtagccgtta
     8941 agtacctcgg gatctacttt aatggcttta tagtttacca ggccgtcctt tacttgatcc
     9001 tgcaatagtt ggtcgtaatt tttgtgtgaa tgatcaaact cctcagctgc actattaggt
     9061 acaccaagat acaaaatagc tataataacg ataaatactt ttaatagttt catctatttc
     9121 ctttccttca gtttgctaat ttagatataa aattatgaag atagattcaa tctatataac
     9181 tgaattctga gaagattgtt tccttgaacc aacaatagaa ctagaatatc aaatggataa
     9241 acataactta agacaggaat aaaatattga aataatatat attaatgtag cagaagaagg
     9301 agtattctct ccttcttctg tattttagaa attacttggg aacaccgggt tcagtcatgg
     9361 taaaagggtc aagcaccctg gcaagctctt tttccggcat cacctttttt tcaagcgcta
     9421 catctctaac ggtctttcct gttgcatatg cctctttagc aattgctgcg gcgttatcat
     9481 atccgataat tggcgcaaga cttgtacaca ttgcaaggct cttttctatc atctcttcgc
     9541 agcgtttttt gtctgcctca ataccgtcaa tacatttatc tgcaaatatg tgtgatacat
     9601 tagtaagaag ccttatggat tctaaaagat tatttgccat cattggcatc attacattta
     9661 attcgaagtt tccagactgt ccgccaattg taattgccgc atcgtttcca atcacctgag
     9721 ctgatacctg tgttactgat tctgcaatta ccgggtttac cttaccgggc attatagaag
     9781 agccgggctg tactgctgga agcataatct ctcctatacc acatcttggg ccgcttccta
     9841 accacctgat atcattagca attttcatca tacttactgc tatagtttta agcgttgcac
     9901 ttgtctgaac aactgcatcc ttggaagcct gtgcttcaaa atgattatct gcttctctga
     9961 attttacttt tgtaattttt gttaagtttc ttgcaatgag acttccaaat ctttttggag
    10021 tattgatacc agtaccaact gcagtacctc caattggaag ctctgagagt tcgtccttca
    10081 ctttctcaag ccttgctatt ccatgctcaa tcatactggc atagccgcta aattcctgcc
    10141 ccagccttat gggggttgca tcctgcaggt gagttcttcc tatctttact atcttatcaa
    10201 actgcctagc tttcttggaa agtgatttct gtagtttttt taatgaaggt ataagactat
    10261 tttgtatctc tgtaagagcc gaaacatgca ttgcagtggg aatactgtca ttgctcgact
    10321 gacccatgtt aatatgatca ttaggatgaa tctttttagc tgattttcct ataatctcat
    10381 ttgcccttgt agcgatcacc tcatttgtgt tcatatttgt agaagtacct gagcctgtct
    10441 gaaaaatatc tacaacaaac tggctgtcca gctttccatc agctacttcc tttgctgctt
    10501 taataattgc gtcgccaaga tttttaggca gaagcccaag actcatgttt gcctctgcag
    10561 cagatagctt tataagcgcc attgctttta taaattcttt tgggagtcct actccgctta
    10621 ccggaaaatt atctattgct cttgcagtct gagctgcata gtaagcatcc tttggtactt
    10681 ccatttctcc catagaatcc gatactgttc tataaccact ctttgccatt ttgttcctcc
    10741 ttaatcacgt aagagtaaat cctcactcta tatcgatatt acaaaatatt ttgaaattaa
    10801 ttttgagacg aataatataa acaactacta ttacaaagtc tacgtattat ttgtatcatt
    10861 gcccgcaaca ggagaaatga atatatatgc caagagctaa aacaggtgaa atagaattaa
    10921 attatgaaat agatggaagc ggagagcaga cgcttgtatt tataaatggt atcacaatgg
    10981 atacaaatgg ctggggctat caggcccctt ttttctcaca attctttaaa gtcgtaagat
    11041 acgactgcag aggacagggg ctttcagata aacctgctaa tccttattcg caagaaatgc
    11101 atgcggatga tcttaaaaat ctcctggatg tcctgggggt ggataaagtt catcttatcg
    11161 ggctatctaa cgggggaatg atcgctcagc attttgctct caaatatcct gacaagttaa
    11221 tatctcttat ccttgtagat acatgctgtt atgtagataa actattgcag cttatgatag
    11281 acacatggat aaaagcaact gagattggag gaaacgaatt cagatatgat atgtcacttc
    11341 ctgttatatt ttctgaaaca tttattagga ataatgaaga aaatttatca caaatgaaag
    11401 agaacaatct tatagcaaac tctccagagg ctataatcaa tcttgtaaaa gcaacagctg
    11461 atcatgattt atctcacagg atacaggaaa taaaatgccc tacactaata atagccggaa
    11521 gtgaagatat actgattcca ccaaaacatt ccagcatact tcatgaaaag atagaaggtt
    11581 cggagtatat tacaatagaa aattgcgggc acgtgccgtc tatagaaaaa ccggcagagt
    11641 ttaatgaaat agttttaaat tttttaacaa aagttaatag cgcttaggaa aatgcggtta
    11701 tttttcaaga tagtttttca taggaaatga tctgtttgct ctttttagtt ttttaagtgc
    11761 tttgacttca atctgtctta tacgctccct tgtaacagaa aactcgtttc cgacttcttc
    11821 taatgtatgt tctttatcct tatctattcc aaacctcatc ctgacaataa gctcttctct
    11881 attgctaaga gcagttgtaa gcgcggtgtc tattaaaccc tttaactctt tatgctcgag
    11941 attttcaagg ggtgattgtg aattatcatc ttcaataaaa tccataagcc tgctgtcttc
    12001 ctcatcacca attggggttt caagagatat tggatctttg gttattttca gcactcttgc
    12061 tactttatca acctgcatac ctgctttttc agaaatttct tctatcattg gctctctgcc
    12121 aagctcctgt actaaagacc gtgaaaccct tagaagttta tttatggttt ctgtcatatg
    12181 cacaggaatc cttatgatcc tcgactgatc ggcaagtgag cgtgtgatgg cctgtcttat
    12241 ccaccatgtt gcatatgtgg aaaatttata gcctcttgaa tattcaaact tctctactgc
    12301 tctcattagc ccgacatttc cttcctgtat taaatccagg aagggaaacc ctcgatttat
    12361 gtatcttctt gcaatactca caacaagcct caggttagat tctataagat cactctttgc
    12421 ttcagatgca attttttcac ctcttagaat ttttctaagt gacctcttta atattttaag
    12481 ctcttctata tgtttatttg cttcattttt ctttttagat tttgatgact ttttatttgg
    12541 cacttcttct agattatttg tactctcaat tgcatttatg cccactgaga ttatcctgtt
    12601 aatctgattt gagctgagat ttagattttc aaggattttt attattttat cattattatt
    12661 tttaatcttg gtctgtactt cttgctgatt tccattttgc gtcttcaata aattttcatt
    12721 atcttttaga tgttttttta tggaatgaaa tgatcttctt atactgtaaa gcacatcctc
    12781 ttcttcatat tcatcggcac catcataata agagtcagta aaatccttaa gcccgacact
    12841 gccctctctg attttctcgc caatattaat aacttcgttt aagagaacag atgaaggaag
    12901 tatagctctt gcaataagtt ttttgccaag ctctattttc tttgcaagat caatttcatc
    12961 ttttctggtt agaagagtgt ggcctgaaat ttcatgaaga taatatttta tcaaatcatg
    13021 ttcattgcca ttttttcttt cctgctgagg ggtggagttt ttgttgtcct tgttttgaac
    13081 ctcatcatcc gaagattcat taaaagatga attttcagtc tcagaagagt ccttatcgga
    13141 agcgccgtta ccaaactcct taaattgttc agatgagagc ttttcagtaa cctcggataa
    13201 ctcctgattt tgttttttgt taagctccat aaacctgctc acttagattt ttttccattt
    13261 ttaccaaatt gtcatattct ttaagcagtt caatttcata atcaggctct ttgttaccag
    13321 tactgttgag ctgtgatttt atttttcgct gctctttttt tatactgcta agttttattc
    13381 tcttatagga ttgttcaaaa atctgttcag caataatttc attttgtata tcttctgatg
    13441 ataaaagcag ctctgagact attctttgag tttgagagtc atccaaatga ttaataatag
    13501 aagccgtatt gttaaagccg gattcactgc aaattttaac aatctgccgg actttgctgt
    13561 cttcaagcat atcaactata tcatcatcta caattttttc tatatactgt ggatagtgca
    13621 agagtacctt aaggaaaata tgctcataag atgaagtgga ttctgaagca ttgcctggct
    13681 gcttgctttt ttcactcgtc tttttgttat tgagaagaga ataaatttcc tgttctctaa
    13741 gaccaaacag ctccgcagcc ttcttggtgt aaacacttct gctaatggaa tcagaataaa
    13801 gggatagctt atcaacaagc tcttttataa taacatttct cgacctgctc cctgagttga
    13861 aatcttctga tatcttatca aaataaaaat caataagaga atttgaattt tcaataaggt
    13921 ttataaattt gtcttttcca atcttttcta caagagaaga tgggtcttca ttttcgggta
    13981 aaactacaac agacggagta attccatgtt tcaaaaacac atcaagcgct cttaatgaag
    14041 cgcttacccc tgatttatca ccatcatata ctatcaccat gttctcgcaa aatctcttaa
    14101 gaagctttgc atgattggat gtaagcgatg tgcccagggt tgctactacg tttttaatgc
    14161 cgttttggaa aagtgataat agatcaatat agccttcaac tattattact ttattttctt
    14221 ttcttatatc atttctggct acatttaaac catacaaaat actgctttta ttgtataagg
    14281 ggctttcagg tgaattaaga tacttgggat catcatcaaa aagagtcctt ccgccaaatc
    14341 cggcaatttt attattaaga tcaaatatcg ggaatattat cctgcctcta aacctgtcat
    14401 aataaccact ctgagatttt gagcttttat taacaagacc caattctgta gccttgttaa
    14461 ggggaacatt tttgcctatc atcctatcgc ttagatcgtt ccagctgttt atactgtacc
    14521 caaggccaaa ctgctcaatg agttcttttt ttaatccgcg tgaagcaagg taatccaagg
    14581 ctttttcacc ctcttgagtt tgatacaaat tttttaaata tattttttga gtaaaattat
    14641 ttattttata taaaagttcg ttattgggct tagcgtttgt tttgcttgat cttgatggca
    14701 actctactcc tgcaatctcg gcaagctctt ttagagccga tacaaagtca aggttattga
    14761 acttcatatg aaaaccgatt atgtctccgc cggttccgca tgcaaagcag tgaaagagcc
    14821 ccttatcatc acttaccctc atagagggtt tgtgatcatc atggaacgga caaactccaa
    14881 gatagtctct gccagaattt tttaagctta taaacttttg aatgtggtct acaatgctta
    14941 aattatcctt aatattctgg actatttcta tatcactgtg ttggttcata ctcttaaatt
    15001 tgatacaaat tcaaagctgt tcacaatgca acaaccaccc cacaaaccaa acataaacac
    15061 atgaaactta agtattgcta ctcaaatgat attatagtaa taatccaata ttattaatta
    15121 catagaccta attgaagtgt attaaaaacg taaatcaata cttttaatat gtcaagcaag
    15181 tagtttagat tgtaatatta tttatcgtct ttatcgaaag aaaatttacc aacgttttct
    15241 ctatttctct ggataatcct ttcttgctct tcatcactta atttatctat ttcaggatcg
    15301 ctatattctt gtctttgctc ttcaaatctc ttgatccagg agtctctttg atctcttact
    15361 gtatctataa gtcccataat taccccctta gttgtatatt taattaacta tttgtgaagc
    15421 tggaataaac tccacataat tttttagttt tggaatcata tctgataaaa ccctaattgt
    15481 ctgcgggtag ggatgacaaa ttccaactgc atagccctgt tttttagaaa ttctaagaag
    15541 ctcattaact ctggctctta taaatctctc cccctctttg cctttatcca aaaacacatc
    15601 cctctcagca gtttttagtc caagtttttt agcctcatca tagcctctgg actgtggtga
    15661 tgtcttgcta tctataaaaa acagtccctc tttcttcact tttttcagaa caagattcat
    15721 aagttcatcg ctttcagtaa acttagagcc catgtgatta tttactccct ttatatgcgg
    15781 aactgctgca aggttctttt caaccagttt cattatctct tttttaggaa gccctgtaag
    15841 aagcgcattc tcacccgcat cgatacccgt ataacccgag gcatactttg gctccatggg
    15901 catatggagc aatacgtccc agcctttact gttggctcta ctggctgcat agtgcgaata
    15961 cttaaggtcg ggtaaaacgg ccatatttat actagcagga attttcagca gatcatctat
    16021 gctttttttg ctcgcgccta tatcatctac gattattgca atttttggtt tggtcttaag
    16081 cccatacaaa aacgcaaact ctttaccatc ggctgcactc ttggtctttg atctattcgg
    16141 cactgcagtg ggtgatatct cttttttggt tatctctggt ttagaaggaa cttttgcagg
    16201 aacaggtttt ggaatttcta aatggaagtt tattttatga gttgatagag taaatatatc
    16261 taggcttgag tttgtagagt ttttgttctt ttttactgtt gtcgttatat ttttaagtcc
    16321 ggtaagactt gcaaattttt tattaaattt attaaactta tcagtattat ctgttttgat
    16381 agaaacttct ttatagtccc agtttatatc gtctttattt cttttataat tatttttaga
    16441 aataatttca gctttcttaa gacctatttc actaaatatt tcgtttaaac gggaatcaat
    16501 tttctttatc tcactactaa gctcatcctc tgttatcctt accctgcttt catcacggga
    16561 gataccggaa tttatcaaat taagcgccgc aagcgtaatg gctacaagga ctataaaact
    16621 cacagtagcc aatattactc gtgtaccttt gttcttccta gcgcggttta ctctttttcg
    16681 tttaggtgca ggtcttttct ttgatcttgc caactatttt tactccaaag actttgatag
    16741 ctttgaattc aatttgctga agttttactg gcgataagct ctgttgccct ttttagctgc
    16801 aggtcactct tagcatcttt attctcaaca attatatccg gcacaactcc gacattgttt
    16861 atagatcttc cgcttggagc ataaaatttt gctgttgtaa tcttaagccc tgaaccatct
    16921 tcaagcctga ttattgtctg cactgatccc ttgccaaagg ttttagtgcc aacaattgtt
    16981 gatctttcat aatcctgaag cgcttcggca acaacctcag atgcactggc actaccattg
    17041 tttacaagta tgacaattgg gaatttatgt gtttcggcac cctttgtcgc atagtaatca
    17101 agattttggt tagaatttct acccttaaca cttactataa ttccttcatc aataaactca
    17161 tccactacct ctacggcctg agtaagcaac ccccctgggt tgtttctcag atccaggatt
    17221 aatccctgta gtttatcatt attttcttgc tgtaatttct ttattgcatt tttaagctcg
    17281 cctgaagttt tatcctgaaa ctgaataagt tttacatagc caaggtttct atccaaaagc
    17341 tctgatttta cgcttttaat ataaataatg cttctttcaa gctcaattac ctttgtagat
    17401 gagtctgctt ttttcttcac tttaatgtta acactggtac caacagggcc tctaagaagc
    17461 tttaccgctt caaactccac catcttactt gtagatttac cgtttatttc aataatttga
    17521 tcacccgctt caattcctgc agctgaggca ggagtatctt ctagcggaga aaccacagtc
    17581 agtactccat ctttaactgt aatctccata ccgagccctc caaactctcc ggatgttcct
    17641 tcttccaaat ccttgaatct gtcgggagtt aaatatgctg agtaggggtc gaggctttga
    17701 agcatgcctt caatggcatt tacagacagt tctgcgctat tgatatcatc cacatagttt
    17761 ctctctacaa gatccaagac ccttgtaaaa gttgaaagcc cctcataaat ttcatcatca
    17821 ccatatacat caggcacata cacaagcatt agtaataaaa gtaagatttt tttcatataa
    17881 acaccttaaa atttattttt aataatatta tagaaagtaa attgagataa aacaaaaaaa
    17941 gaatgaaaat attcttaaat aaggcaggta atattatttc gatttaagca gctgctcaca
    18001 agactctgat atattagcag ctttgagacc aaaatattct aacagcttca taccgtttcc
    18061 ggatgtacca aaacggtctt tcatacctat tctctttaca agtgtagggc agtgttcgga
    18121 tagcacctct gaaacggctc ctccaagacc acctataata ttatgttctt ctgctgtaac
    18181 aacggctcca gtcttttctg ctactgaaac cactgtttct atgtcaatag gttttattgt
    18241 agcaagattc actacacatg cgtctatccc tttttcttcg agcagttttg ctgcattcaa
    18301 cgcttcactt accataattc ccgcagcaaa tattcctaca tcatttccat gtcttaatat
    18361 acttgctttt ccaagctcaa atttatattt atcattataa atagtgggaa actttgctct
    18421 tgcaagccta acatatgccg gtccttcatt gtcagccatt gtccttatta cctgctcagt
    18481 ttctacggca tcagctggaa caatgactct catattgggc agcactctca taagacatat
    18541 atcttcaagc gcttgatgtg tggcaccatc ctcaccaact gtaacccctg catgggtggc
    18601 aacaatcttt acattcaaat taggaaacgc tatagattgt cttacatgct cccaggctct
    18661 tcccgttgca aagacagcaa aagtactagc aaaaggtctc tttccggcaa gggcaaaacc
    18721 tgcggctgtg gccatcatat cctgctctga aactcccatg gtgaaaaatc tatcgggata
    18781 ttcttttcca aacagattgg tttttgtaga aaccgtaaga tcggcatcca gtaccacaat
    18841 gtccgggtga ctggctccaa gctctaccag tgtttttcca tatacttctc ttatgtatat
    18901 actttcaccg ggttgctcta aaacataatc actatactca gatttgctca tgcggcctct
    18961 ccaagttcct ttagagcaat ttttagttct tcatcagatg gggcaacccc gtggtagttg
    19021 cctttatttt caaaaatgga aaccccttta cccttaatcg tatttgcaat tattgcatag
    19081 ggtttacctt tagtagattt agccctatca aatgctttta taagactttc aatatcatga
    19141 ccgtctttaa gctcttcagt ttcccagcca aaagcaataa atttatcagc aacggggtta
    19201 atatctttga tcttatccat agcaccatca ttctgaaatc tattcctatc tactatggcg
    19261 cataagttat cgagttttct atacccagaa gtggtaatag cttcccatgt agaaccctct
    19321 tgcagttctc cgtcacttag aagcgtatag accctgtagt ctttattatc taatttgccg
    19381 gcaagcgcaa tcccatttga tgcggaaagc ccgtggccaa gt
//
LOCUS       DBNGSV010000056         3032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1880806, whole genome shotgun sequence.
ACCESSION   DBNGSV010000056 DBNGSV010000000
VERSION     DBNGSV010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1880806"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..910)
                     /locus_tag="AC159U_02150"
     CDS             complement(59..910)
                     /locus_tag="AC159U_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335888.1"
                     /translation="MFNLLKSKTIFTLFLFAVFIAAGSNLLLADDRGSHKQGLHFAHP
                     LITESPSPDTKIRLDYIFKDIEGEGHGDEEEDGEGGGLNEHSLQLEAEYAFSRNFSIE
                     VGVPYTFVDPDDGETEDHFNNIEVGLKFATYIFEEYGLLLGGGLEFGIPSGDDERGIG
                     SDHIIEIEPFLSMGYKYHDFELVSFLSLGFPVNQDDGKDESDEFGYNVSLLYHLTPSI
                     EALLEFDGEVALNGEEDGESVLNISPGIKVKPFTNKNIQIGFSGDLPPYNSTRLQVRI
                     LGNKPYE"
     gene            complement(965..1372)
                     /locus_tag="AC159U_02155"
     CDS             complement(965..1372)
                     /locus_tag="AC159U_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335889.1"
                     /translation="MLYGSKKLLYKCFSLTLIIFLLLLTVSGFSKSYIPVKTQDNTTA
                     IVEGCHEQVNHSILKELILNTFQSADDEECCEKICFCCSKNIRGILKFVLFKSTFINP
                     DLKAAFFRTSENLILIKNTLGAFGNKSPPTVLS"
     gene            complement(1448..2173)
                     /locus_tag="AC159U_02160"
     CDS             complement(1448..2173)
                     /locus_tag="AC159U_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR3335890.1"
                     /translation="MPHLKDINTRAPKKADRDDLEYKTELLYKRLQSFQHSLYKNKKH
                     SVLIVLQGLDAAGKDSTIRSVFTGVNPMGCFVKAWKVPTAEEFAHDFLWRIHEHVPPK
                     GMIHIFNRSHYEDLIVPKINDSISDKQFAGRCKFINTFEELLIEENNTIIFKFFLHVS
                     QEEELERLMERKNDPEKRWKYNPKDFEVNKQWSEYIDHYDEIIRKTNKKAKWHVIPAD
                     QKWYRNYLVATTLVNELEKKLED"
     gene            2241..3011
                     /locus_tag="AC159U_02165"
     CDS             2241..3011
                     /locus_tag="AC159U_02165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3335891.1"
                     /translation="MKCLIVSDLHYRLNHFDWVLNESKNFDLCIIAGDMLDVASIASP
                     KAQITVVTKYLSAINENTNLIVCSGNHDLDARNTDGEKYSRWVFKSNELGIPADISNV
                     TLNGCMFTILPWWDGPVLLKDIEEKIAKDSSLDKKKWVWIYHTPTDNSQTSWDGKKFN
                     GDENLNTWIEKYRPDMVISGHCHLSPFKNDGSWVDQLKSTWVFNPGMQIGPFPTHIIL
                     ETELNKAAWFSLEGGEEIDLSIELTRPVSELRQMPDWI"
BASE COUNT          942 a          596 c          509 g          985 t
ORIGIN      
        1 ttaaatatct tattatttgc tctggcgtgt aacgcttcct gggcatttcc cttgcctcct
       61 attcataggg tttattacct aaaattctaa cttgtaatct ggtactatta taggggggaa
      121 ggtcacctga aaacccaatt tgaatattct tgttggtaaa gggcttaacc tttattccgg
      181 gagaaatatt gagtacagat tcgccatcct cttctccgtt tagtgcaacc tctccgtcaa
      241 attccaaaag cgcctcaata cttggagtta gatgatatag aagtgaaacg ttataaccaa
      301 actcatcact ctcatcttta ccgtcatcct ggttcacagg aaaaccaagt gatagaaatg
      361 agacaagttc aaaatcatga tacttataac ccattgataa aaacggctct atttcaataa
      421 tatggtcgct tcctattcct ctttcatcgt cgcctgaggg tatgccaaat tctaaccctc
      481 cacccagaag caatccatac tcttcaaata tatatgtggc aaatttaagc cccacttcaa
      541 tattattaaa gtgatcttca gtttcgccgt catcaggatc aacaaacgta taaggcaccc
      601 ctacttcgat gctgaagttt cttgaaaaag catactctgc ttcaagctgc aaagagtgtt
      661 cattaagccc tccgccttcc ccatcttctt cctcatctcc atgtccttca ccctcaatat
      721 ctttaaatat atagtcaagc cttatctttg tatcaggcga tggtgactca gttatcaatg
      781 gatgagcaaa atgcagcccc tgcttgtgac tccctctgtc atctgctaat agcaaatttg
      841 agccagcagc aataaaaaca gcaaataaaa acaaagtaaa tatagttttt gattttaata
      901 aattaaacat agtactcctc ccctttaatt atttatgaat tttataaagc aagaatttta
      961 tatttcagga aaggactgtt ggaggagatt tatttccgaa agcacctaat gtatttttga
     1021 ttaatattaa gttttctgaa gttctaaaaa aagcggcttt aaggtccggg ttaataaaag
     1081 tagatttaaa taaaacaaat ttaagtattc ctctgatatt cttactacag caaaaacata
     1141 tcttttcaca gcattcttca tcgtctgctg attgaaatgt atttaggatc agttctttaa
     1201 gaatggaatg atttacctgc tcatgacagc cttcaacaat ggcagttgtg ttatcctgag
     1261 ttttaactgg tatgtaggat ttactaaaac ctgaaacggt aagaagaagt aaaaaaataa
     1321 tcagtgttag agagaaacac ttatatagca gtttttttga accatacagc aattaacaag
     1381 cccctttata taaaacaata tataaaatat gacgtttata atggtaaaaa gattctaaga
     1441 tagagaatca atcctctaat ttcttttcca gctcatttac aagagtggtt gcaactaaat
     1501 agtttctgta ccatttctgg tcggcaggta tcacatgcca tttggctttt ttattagtct
     1561 tacggataat ttcatcgtag tggtcaatat attcactcca ctgtttattt acctcaaaat
     1621 cctttggatt atatttccac cttttttccg gatcgttttt tctttccatt agtctttcaa
     1681 gttcttcttc ctgtgacacg tgcaaaaaga atttaaaaat tatggtgtta ttttcttcga
     1741 ttaaaagctc ttcaaatgtg tttataaatt tacaccttcc ggcaaactgc ttgtccgaaa
     1801 tcgagtcatt aatctttggc actatgaggt cttcataatg tgagcggtta aatatatgga
     1861 tcattccctt tggtggtaca tgctcatgaa ttcgccagag aaaatcatga gcaaattctt
     1921 ccgctgttgg caccttccat gcttttacaa aacatcccat gggatttact cctgtaaaaa
     1981 cactccttat ggtgctgtct tttccagcag catcaagtcc ctgcaggact ataagaacag
     2041 agtgcttttt gtttttgtat aaactgtgct ggaaactctg aagtctctta taaaggagtt
     2101 cagtcttata ctcaagatca tctcggtcgg cttttttagg cgctcttgta tttatatctt
     2161 ttaagtgcgg catttttcta ttttatacat aatttatttt atttgccaga taaatctttc
     2221 tgtgtttaat ataagaagta atgaaatgtc taatagtttc cgatcttcac tacagactta
     2281 accacttcga ctgggtatta aatgaatcca aaaattttga cctgtgcata atcgcgggtg
     2341 atatgctcga tgtagcttct attgccagcc ccaaagcaca aataaccgtt gtaacgaaat
     2401 atttaagtgc aataaatgaa aatacgaatt taatcgtatg ctcgggtaat catgatcttg
     2461 atgccaggaa cacagacggt gagaaatatt caaggtgggt ctttaaatca aacgaacttg
     2521 gtattccggc agatataagc aatgtaactt taaacggctg tatgttcaca atccttccct
     2581 ggtgggacgg ccccgtttta cttaaagata tagaagaaaa aattgcaaaa gattcctcgc
     2641 tagataaaaa gaaatgggtc tggatctatc acacacctac agacaattca caaacaagct
     2701 gggatggaaa aaaatttaac ggcgatgaaa atctaaatac atggattgaa aaatatagac
     2761 cagatatggt tatatccggg cactgccatc tctcaccttt caaaaatgac ggctcatggg
     2821 tagaccagtt aaagtccaca tgggttttca atcccggaat gcagataggc ccatttccaa
     2881 ctcatattat tttagaaacg gaattaaata aggcagcatg gttttccctt gaaggtggag
     2941 aagaaatcga cttgtccatt gagttaacaa ggcctgtatc cgaacttaga caaatgcctg
     3001 actggattta atcagcatct ttactccttt ct
//
LOCUS       DBNGSV010000057         5821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1742451, whole genome shotgun sequence.
ACCESSION   DBNGSV010000057 DBNGSV010000000
VERSION     DBNGSV010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1742451"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="AC159U_02170"
     CDS             <1..691
                     /locus_tag="AC159U_02170"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3335892.1"
                     /translation="NETSGTQSYGDPIIRKDPIIDEDSIIEEPAKSFNLHNRGSQIKI
                     IKDSNSKNVIALVSKISDYENLTRTYDASELALLLTDYRKTASAIVSQYGGMVETLVR
                     DELVALFNVTENLEKPELRAVSAGVELLHTLVELNKHKVREGDEQISCRIGVYSSSIP
                     VSKESGTPSNLGLAIEPAKEICECSSDWKLMLSPNVYSSVKDYVEVTPPPNGKHSNYS
                     VVTVEQGVINI"
     gene            831..1883
                     /gene="rlmN"
                     /locus_tag="AC159U_02175"
     CDS             831..1883
                     /gene="rlmN"
                     /locus_tag="AC159U_02175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3335893.1"
                     /translation="MKANIKDFDPKELETFIYELGEKPYRAKQISAWIFAKNARTFSE
                     MTDLSLPFRDKLQESAEIRSTLNLIGEQKSVDGTKKYLFELNDGNKIESVLIPDKKRN
                     TLCISSQVGCAMGCTFCLTARVGKIRNLSCSEIVDQYLAVNSLNDGCVTNIVYMGMGE
                     PLDNLENTVKSLNIFTDSNFIGLSPRRITVSTSGLVHKIKELSEKVSVNLSVSLNAPS
                     NAIRDEIMPVNKRFPISTLLKECKRFPILRRKFLTFEYVIIKDLNDSTANAHELAKLL
                     KGINCKINLIPFNEAAPITYKTPQIETVLEFQKILNSYGLNVKIRRSRGSDILGACGQ
                     LAATYPLDNKTKKVVH"
     gene            1892..4525
                     /gene="alaS"
                     /locus_tag="AC159U_02180"
     CDS             1892..4525
                     /gene="alaS"
                     /locus_tag="AC159U_02180"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3335894.1"
                     /translation="MTGKEVRDTFLKYFEQKDHTIVKSSSLIPKNDPTLLFTNAGMVQ
                     FKDVFLGLEKRDYNKATSSQKCVRAGGKHNDLENVGVTARHHTFFEMLGNFSFGDYFK
                     EKAIEFGWELITEVYKLPKSKLWVTVFRDDDEAFDLWNKKMGLPKSRVLRMDEKDNFW
                     SMGDTGPCGPCSEILIDQGEAFGCGMPSCAVGCECDRYLELWNLVFMQFDRDAKGNMT
                     KLPNPSIDTGMGLERITAVIQGVKSNYETDLIREIISKFEQLTATEYGRDEKTDISLR
                     VVSDHIRALTFLISDGVLPSNEGRGYVLRRILRRAVRHIRKLDVTEPTLFKASELLSE
                     KMGDTYPELNERLSFVTSVIKNEEERFFETIDRGLELLNLEIEKHKDDKNIPGDFAFK
                     LYDTFGFPLDLTEDIAKDSDLTVDVKGFNDNMEVQKNKSRKAWKGSGEEQLIPLYKEF
                     SSGGLSTEFTGYSKLSDEGRITAIISSGQLIDNANEGQTVEVICDRTPFYGESGGQVG
                     DRGTITSENGEVEVLDTQKPLINLFVHTCMVKKGTIWVGDKAQLKVNKKFRQGAKSHH
                     TATHILQATLKEIVGDHIAQAGSYVGPERLRFDFSHFESIDSKKLRKIEEIVNSRLRL
                     DDSVITEADVPYDEAVKSGATAIFEEKYGDKVRVVSIGDYSKELCGGTHLEGTGEAGL
                     FKIITETSSAAGVRRIEALTGEACWNYMRGYEDKITQASEILKSPPSDLIPRLKKVIE
                     ENEKYKKELDEIRSKSISSQTQDLIKNAKEIDGVNVLSMEIPDSNPNELRKVWDDLKS
                     KNKALVAVLGSKSADKAFLLAGITNDIKNKYNAGKIISEIASIVGGKGGGRPDMAQAG
                     GTKPEKLNEALDSVYSVISGN"
     gene            complement(4526..5461)
                     /gene="rpoH"
                     /locus_tag="AC159U_02185"
     CDS             complement(4526..5461)
                     /gene="rpoH"
                     /locus_tag="AC159U_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR3335895.1"
                     /translation="MKKKKNETKIEPEIEVEPAGKTEPISHTQLPATTNTIERYLAEI
                     SNHPVLSREEEHKLAMKYKEDGDLEAARQLIRANLKFVVKVANEYKNYGINPMDVIQE
                     GNLGLMQAVKRFDPTRGYRLISYAVWWIRAYIQNYIVKSWSLVKVGTTQAQRKLFYKL
                     RSTKNEMDLSKQVLSAEDYKLLANKLGVSDDAVIEMDKRMRTKDFSLDTEIKDKSEAT
                     HLDFVEDKHLGQDDIVSKSEQEVAVQQGLTDALELLNEREQFIIKNRVLTDTPLTLEE
                     IGQKYKISRERVRQIESAALKKIRGVFEQMGIEGI"
     gene            5664..5739
                     /locus_tag="AC159U_02190"
     tRNA            5664..5739
                     /locus_tag="AC159U_02190"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5697..5699,aa:Asn,seq:gtt)
BASE COUNT         1879 a         1020 c         1230 g         1692 t
ORIGIN      
        1 taatgaaaca tccgggactc aatcttatgg tgatccaata atacgcaaag acccaataat
       61 agatgaagat tcaataatag aggagcctgc aaaatcattt aatctacata atagaggcag
      121 tcagataaaa ataataaagg attcaaacag taaaaatgtt atagctcttg tatcaaagat
      181 aagtgattat gagaatctta caagaacata tgacgcctcc gaactagcgc ttttactgac
      241 agattacaga aaaacggctt ctgctatagt gtcacaatat ggcggtatgg tggaaacact
      301 ggtaagagac gagcttgttg cgctatttaa tgttactgaa aatctagaaa aacctgaact
      361 ccgagccgta tctgcagggg tagagcttct tcatacgtta gttgaactaa acaaacataa
      421 agtaagggaa ggcgatgagc agataagctg caggataggt gtgtattcaa gctctattcc
      481 ggtatccaag gaaagtggta ctccatctaa tcttggtctt gcaatcgaac ctgcaaaaga
      541 gatttgtgag tgttcatctg actggaaact tatgctctca cccaatgttt atagctcggt
      601 aaaagattat gtagaagtta caccaccccc aaatggtaag catagcaatt attcagttgt
      661 taccgtagaa cagggtgtta taaacattta gtcttaaaat atatcctgtt cctttcattt
      721 aacctgccat atctttatct tttaataatc ttataattat agttttgttt taattattat
      781 tagctgttaa tattaagcca aattatattt actattgatg cgcataaatt atgaaggcaa
      841 atattaagga ctttgatcca aaagagcttg agacgtttat atatgaactg ggcgaaaaac
      901 catacagggc aaagcagata agtgcgtgga tttttgctaa aaatgccaga acattttctg
      961 aaatgaccga cctttctctg ccatttaggg ataaactgca agaatctgca gaaatcagat
     1021 caactctaaa tcttataggt gaacaaaaat cagtagatgg taccaaaaaa tatctgttcg
     1081 agctcaatga tggaaacaag atagagagtg ttttaatccc ggataaaaaa agaaatactc
     1141 tgtgtatttc ctcccaggta gggtgtgcaa tgggatgtac attctgtctt acggcaaggg
     1201 tggggaaaat tagaaaccta agctgctcag aaattgtaga tcagtatctg gctgtaaact
     1261 ctctaaatga tgggtgtgta acaaacattg tatatatggg aatgggtgag ccgcttgata
     1321 atcttgaaaa tacggtaaag tctctaaata tttttactga tagcaacttt attggtcttt
     1381 ctccaagaag aataacagta tcaacctccg ggcttgtaca taaaataaaa gagcttagtg
     1441 aaaaagtgag tgtaaatctc tcagtttctc taaatgcacc ctcaaatgcg ataagagacg
     1501 aaataatgcc tgtaaacaaa cgttttccta ttagtactct tcttaaggag tgtaagcggt
     1561 ttccaattct tagaagaaaa ttcctgacat ttgaatacgt tataattaaa gatctaaatg
     1621 acagcacagc aaatgcgcat gaacttgcaa agcttctaaa aggaataaac tgtaaaataa
     1681 atctaattcc atttaatgaa gccgcaccaa taacgtacaa aactcctcag attgagacag
     1741 ttttagagtt tcagaaaata ttgaattctt acggtctcaa cgtaaaaatc agaagaagcc
     1801 ggggcagtga tatacttgga gcctgtggtc agcttgcagc aacttacccg cttgataata
     1861 aaactaaaaa ggttgttcac taactgaaat tatgaccgga aaagaagtaa gagatacatt
     1921 tttaaaatat tttgagcaaa aagatcacac gattgtaaaa agctcatcac taatacccaa
     1981 aaacgaccct acactgcttt ttacaaatgc aggtatggtt cagtttaaag atgtattctt
     2041 gggattagaa aaaagagatt acaacaaggc tacctcatca cagaagtgcg taagggcagg
     2101 gggtaagcac aatgaccttg aaaacgttgg agtaactgca agacatcaca ccttctttga
     2161 gatgctcgga aacttttcgt ttggagatta tttcaaagaa aaggcgatag agtttggctg
     2221 ggagttaata acagaagttt ataaacttcc caaaagtaag ctttgggtaa cagtattcag
     2281 ggatgatgat gaggcctttg atctctggaa caaaaaaatg ggccttccaa aatccagagt
     2341 cctgcgaatg gatgaaaagg ataacttttg gtctatggga gatacggggc cctgcggtcc
     2401 atgctcagag atacttatcg atcagggaga ggcatttggt tgtggaatgc cttcttgtgc
     2461 agtgggatgt gagtgtgacc gctacttaga actctggaac ctggtgttta tgcagtttga
     2521 ccgggatgca aagggcaata tgacaaagct tccaaaccca agcatagata ccggaatggg
     2581 acttgagcgg atcactgctg taattcaggg agtaaaaagc aactatgaaa cagatctaat
     2641 aagagaaatc atatcaaaat tcgagcagct tacggcaaca gagtatggaa gggatgaaaa
     2701 aaccgatatt tcactgaggg ttgtatccga tcacataagg gctcttacat ttttgatctc
     2761 cgatggtgtc ctaccctcca atgagggaag gggctatgta cttaggcgaa tacttagaag
     2821 ggcagtaagg cacattagaa agctagacgt tacagagcct acactgttta aggcatctga
     2881 actattgtct gaaaaaatgg gagacaccta tcccgagctt aatgaacgtc ttagttttgt
     2941 gacttctgtg attaagaatg aagaagagcg attttttgaa accatagaca gagggcttga
     3001 gcttttaaac ctggagattg aaaagcataa agatgataaa aacatcccgg gggattttgc
     3061 ctttaagctc tatgatactt ttggatttcc gctcgatctt actgaggata ttgcaaaaga
     3121 cagcgatctt acagtagacg tgaagggatt taatgacaac atggaagtac agaaaaataa
     3181 gtcccgtaag gcgtggaagg gctcagggga agaacagctt attcctcttt ataaggaatt
     3241 ctcatcagga ggtctttcaa cagaatttac aggatacagt aaactttcag atgaaggcag
     3301 gataacagca ataataagct cagggcagct cattgataat gcaaatgaag ggcaaacagt
     3361 agaagttata tgtgacagga cgcctttcta cggtgagtcg ggtggtcagg ttggtgacag
     3421 agggactatt acatctgaaa acggagaggt agaagtgctg gatacacaaa agcctcttat
     3481 taacctgttt gtgcatacat gtatggtaaa gaaaggaact atctgggttg gtgataaagc
     3541 tcagttaaaa gttaataaga aattcaggca gggagcaaag tcacatcata cagcaacaca
     3601 tatccttcag gcaacactaa aagaaatagt aggagatcat atagctcagg caggctcata
     3661 tgtgggaccg gagaggctaa ggtttgattt ctctcatttt gaatcgattg atagtaaaaa
     3721 attaagaaag attgaggaaa tagtaaactc gaggctgagg ctcgatgatt cagtgatcac
     3781 agaggcagac gtgccttatg atgaagcagt taaatccgga gcaactgcta tatttgaaga
     3841 aaagtatggg gataaagtga gggttgtctc aattggagac tacagcaagg agctttgcgg
     3901 aggaacccat cttgagggca ccggagaagc cggtctattt aaaataatta ccgagacatc
     3961 ttctgcagct ggtgtgagaa ggattgaggc actcacgggt gaggcatgct ggaactatat
     4021 gcgtggatat gaagataaaa ttactcaagc ttcagaaata ctaaaatcgc cgccttctga
     4081 tttaatcccc aggcttaaaa aagttattga agagaacgaa aaatataaaa aagagcttga
     4141 tgagataagg tcaaagagta tttcttctca aactcaggat ttaatcaaga atgcaaaaga
     4201 gatagacggg gttaatgtgc tctcgatgga gatacccgat agcaatccaa atgagctaag
     4261 aaaggtatgg gatgatctaa agtctaagaa taaggctctg gttgctgtgc ttggaagcaa
     4321 atctgctgat aaagcctttt tgcttgctgg tattacaaat gatattaaga ataaatacaa
     4381 tgccggcaag atcataagtg aaatagcttc tattgttggt ggtaaaggtg gtggcagacc
     4441 cgatatggct caggcaggcg gtacaaaacc ggaaaagtta aatgaagctc tggactcagt
     4501 ctattcagtt ataagcggta attaactata tcccttcaat tcccatttgt tcaaatacac
     4561 cccttatttt cttaagtgca gcgctttcta tctgacgcac tcgttctctg gagattttat
     4621 atttctggcc aatctcttcc aaagtaagcg gagtatctgt aagaaccctg ttttttataa
     4681 taaattgttc cctttcattt aatagttcaa gtgcatccgt gaggccttgc tgcactgcaa
     4741 cttcctgctc tgattttgat acgatatcat cctggcccaa atgtttatct tctacgaagt
     4801 caaggtgagt agcctcggat ttatctttta tctcagtgtc gagtgaaaag tctttggttc
     4861 tcatcctctt gtccatttct ataacggcat catctgaaac tccaagcttg tttgccagaa
     4921 gcttgtaatc ttcagcacta agcacctgct tactcagatc catctcgttt tttgtagacc
     4981 gtagcttata aaaaagcttg cgctgagcct gtgtagttcc aacttttaca agactccagg
     5041 acttaactat atagttttgt atataggccc taatccacca cactgcatag gatattagcc
     5101 tgtaacccct ggtaggatcg aatcttttta cagcctgcat gagtcccaga ttgccttcct
     5161 gaatcacatc catggggttt atgccgtagt ttttgtattc atttgccact tttactacaa
     5221 atttaagatt ggctctgatt aactgtcttg ccgcttctaa gtcaccatcc tctttatact
     5281 tcatagcgag tttgtgttct tcttctctgc ttagaacggg gtggttgctt atttcagcca
     5341 ggtatctttc aatagtatta gtagttgcgg gaagctgtgt atgagagata ggttcagttt
     5401 tcccggccgg ttctacttca atttcaggct caattttagt ctcattcttt ttctttttca
     5461 tttcgatttt tcttaattgt attactaaaa catattcact ttagatgaag ttagcaatag
     5521 caggaatccc taaaattata attactgaag ctattaagta aataattatc tatttcaggt
     5581 ataatccata ttggtttaca agcatatata atgcttgact gaaattattg tttagataaa
     5641 atgtaattct ttttttattt agttccggag tagctcagct ggcagagcgg gtgactgtta
     5701 atcactaggt cgggggttcg agcccctcct ccggagccaa atattttaac taatctcatt
     5761 aattttttcc taaaattaag cttaagttta ttagtatttt tcatttaata gctattttat
     5821 t
//
LOCUS       DBNGSV010000058        20040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1119814, whole genome shotgun sequence.
ACCESSION   DBNGSV010000058 DBNGSV010000000
VERSION     DBNGSV010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 20040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20040
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1119814"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC159U_02195"
     CDS             <1..573
                     /locus_tag="AC159U_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024105.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL/Epsl periplasmic domain-containing protein"
                     /protein_id="HIR3335896.1"
                     /translation="LYGSDTMAGKLNYRKDEFKFTSKDEETRKALYIPALLLAALFVL
                     VLVRNSINYIRVNSEYKKLSSQIEKSVKEVFPEVKVIPRPVDFMQNQVTRVNSELDLI
                     AGIKSGKTPLDIMRTLSTTIPISSSVTFDELNFTDDKSLRLKGKSKSYDDIAKVEKAL
                     NDSGHFENVVRSSAGSALDKIKFEISLEIK"
     gene            573..1172
                     /locus_tag="AC159U_02200"
     CDS             573..1172
                     /locus_tag="AC159U_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335897.1"
                     /translation="MDFSKIKELFAGIRESIESNINISELTERDRYIIYGVAGFLAVI
                     IIYLAVFSFSSKVSNLEKRVLTLETDLQRVADLKSEYIESSRQLKNLVKSGSKQTGPL
                     ISVVEKVLLSESVERKNFSIKDRNQRSKDAEDIYNEKSVEVQIKQIPLGKMIDILYAF
                     QSKNTNLKVKGLRIRTRFDNSNSVDIYFMVSTFEFKEVS"
     gene            1175..2098
                     /gene="gspN"
                     /locus_tag="AC159U_02205"
     CDS             1175..2098
                     /gene="gspN"
                     /locus_tag="AC159U_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HIR3335898.1"
                     /translation="MSILKNKKLLFVLTFIFVFICFFYIQFPNDRLKRRLEFEIEKNT
                     QFDAQIDKIKILPLLKIQINGLKLSRNDQQILISKAVIKPSILSLLSKDIIVKYDINV
                     FKGSAQGDIIITKDSGQLQSMIVKVESLDVDEFTQIYSHSAKENIEIKGLLSGNMNLS
                     SNNTGDFDFYIEDLDILKIKIKTFDLPQFTDLRSNLKGQIYRDRTIINELKFDNEDIT
                     LKLKGSTPPLWRLSKGSIDLLYRVDVRSKKYALLKSFLNKDNKGNVAGKIVGSLQKPQ
                     LKNVNDSNAQPKRQQGPRFRKTFSPKKQQAI"
     gene            2233..3312
                     /gene="pheA"
                     /locus_tag="AC159U_02210"
     CDS             2233..3312
                     /gene="pheA"
                     /locus_tag="AC159U_02210"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3335899.1"
                     /translation="MASKIKLKDLRDQIDEIDKKLLSLLNLRAGVALKVSEFKQKNAV
                     DVYDPVREKQVLEKIISENKGPLNSDLVKSVFRQIISVCRSIQKPIELTVLGPPGSYS
                     HQVATKILGVSVDINPVATIEDVFVDLQKGKNLFGVVPVENSAEGSVGNVLDKFIDTD
                     IKICTEHYERISHSLLSKDGKIENIKTVASHPQALGQCRKWLSKRLKNIDFKETSSTA
                     KAAEIASENKHIAAIAGEFNTSIYDLQVVESSIEDYPNNTTRFFLIGYQENSPSGNDK
                     TSIVFSLKDEPGALSSVLEPFDKKNINLTKIESRPTRTKPWEYVFFVDFEGHSSEKSI
                     QRILNNVRKSCVFLKILGSYPKGKV"
     gene            3337..4419
                     /gene="hisC"
                     /locus_tag="AC159U_02215"
     CDS             3337..4419
                     /gene="hisC"
                     /locus_tag="AC159U_02215"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3335900.1"
                     /translation="MKPNEYVRDLVPYLPGKPVEELQRELGITGAIKLASNENPVGAS
                     PKALKEVASKLSDINWYPDGDCFHLKNELSKKLGFQNENIVVGNGSNDIIEIAARTFM
                     KPGDEAVMGEYAFIVYPLVTKAIGADAVISPMPDLIHDLRDMYKRITDKTKMILIANP
                     NNPTGTMVNRDEMEWFLQKVPDDVLVLVDEAYFEYVNDKNYPNTLEYLSNRPSLITVR
                     TFSKIYGLAGLRLGYGISSEQIISYMNRVREPFNVNSLAQSAGVAALNDKEHVDNSNK
                     INSEGRKYLIKELEKIGIEYYDSYTNFILIDLKRDPMAVYNELLKLGVIVRPVGGYGL
                     KTHLRVTIGLLEQNIRFIKSLKKVLD"
     gene            4431..5258
                     /locus_tag="AC159U_02220"
     CDS             4431..5258
                     /locus_tag="AC159U_02220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3335901.1"
                     /translation="MQFNKIIIIGLGLIGGSLALALKKSGQVKHVVGVDVLPESVEIA
                     LRKGIIDEGQVGIDEVVYDSDIVVIATHVGSIVDIARAISVSDSTVVTDTGSVKENIV
                     NEIENSCNLRFIGSHPIAGTEKSGVSNVVDGLFKDKITIITPTNNSTSEATETVTRMW
                     TVIQSKVIRLSAQKHDEIFAHVSHLPHVCAFSLVNSLIKKDSTLFNYGGGGLKDFSRI
                     AESSPDMWVDIFIYNKDEILKAIDEYKDSLSEIENYIKKSDKKSLKNILGSSSNIKK"
     gene            5334..6083
                     /locus_tag="AC159U_02225"
     CDS             5334..6083
                     /locus_tag="AC159U_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3335902.1"
                     /translation="MKILLSVIIALAVGTIALNAWILSEVSNIKASVAKQDEEIAKLS
                     KAIAARPAAPAAQPAAADPQNVKVSIDDDPIKGDLKAPVTIVEFSDYECPFCKRNHDQ
                     VFSQIKKEYIDTGKVRYVFRDYPLGFHKKAIPAAIAANCAGEQGKYWEINDFLFQSKA
                     NLSDEKYIEFAKNNNLDMEKYQACLKDKKQEAEVDKDFKDGQAYGVRGTPSFFIGKTQ
                     EGKEMTGAYIRGARPFDAFKTEIDKILSAQK"
     gene            6462..7316
                     /locus_tag="AC159U_02230"
     CDS             6462..7316
                     /locus_tag="AC159U_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3335903.1"
                     /translation="MVYTNLTPDSGGFKKLNTPWGKTQKQKIKYGSTKYSDGYNKEIL
                     KASRQYSLDPNIIKAIIKIESNFDPKAVSRKGAMGLMQLMPGTALGLGVSNPFDPGQN
                     IRGGTKYFKKLMNMFNGNIKLALAGYNAGENAVIKYGYSIPPYNETENYVEKVLYHYN
                     NLKGKNSYNYNTGQTSSLVVKTSARSNNYKNTDVFDEEQLRGELSGKFLIQLASYPKL
                     GLAKELENTLKSKGYPAFIQKAYLPNSGTWYRVRIGSFTTKHEAQQFKNSLKSTEPFI
                     NDAIVVNL"
     gene            7450..9141
                     /locus_tag="AC159U_02235"
     CDS             7450..9141
                     /locus_tag="AC159U_02235"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR3335904.1"
                     /translation="MSNKPSEYTPPVQDNEFITTITDKPEDLLEVGVLFIGAGPASLA
                     GAIRLAQLLENEPEIMESLGEIPIAVLEKGKYPGAHLVSGAVVNPIAIRRLFPELKDS
                     DFPFLQEVNGESLYFMTENMRIPLPTPPTMKNHGNFVASISQLAAWLGEKAEELGVMV
                     FNEMSAVKLIVEDNVVKGVRTDDKGADKDGNPLENFQPGSDILAKATVLGEGTTGHLS
                     MALFDHFEIPRENPQIYALGVKEVWEVAKPLGEVIHTMGWPLSTSSKNNEFGGSFIYP
                     MGDDKVCIGLVVGLDYKDASLSVHDLLQQLKSHPMVKKILEGGKKADKGWGAKTIPEG
                     GYYAVPDKLNVPGAVVVGDSAGLVNVPALKGIHYAMMSGMLAAESIFNSLKQKNDLQT
                     YSGLDSYDAAVRESFIMKDLYEVRNMRQAFQYGFIPGFILSGLMTVTKGKFPGGKFTG
                     HHDSEQEMFIGQRDYPKPDNEYYFDKLTSVFDSGNRTRDNQQDHVRIEKEVSEEVGEA
                     WINMCPAQVYEWHEEGGKKVIRTDPTNCIQCGAITSKGGRLTPPEGGSGPEYTQT"
     gene            9253..10482
                     /locus_tag="AC159U_02240"
     CDS             9253..10482
                     /locus_tag="AC159U_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR3335905.1"
                     /translation="MDLNEIYNNASAILLSSQNCWIDESYLYVFSGKPEFTLYLTDNN
                     LFLGKNNEITELFGVNPFDFIEQQAANGKYAAGYISYNYLEYTDIGLTLRNKKFNDNF
                     PLLYFNFYSPAKLDKFNIKQAEKILNIKQKEIRSKQSDIQYSTHKEGYILDINKIKEY
                     IENGDVYQVNLSHKITTRLQNNPLNTFFNYYKAQPVPYGIYIDCDKFHFLGGSMEQFV
                     EKRGDIITTKPIKGTSKRNQHKSKDDQIKANLSTNPKEKAENLMIVDLMRNDLSRVCK
                     PATVKVNKLFNVKSYRTLHQMESEITGQLLQDKRLKDIIYSVFPPGSVTGAPKKRSIQ
                     IIDELENHSRGPYCGCAGVFSPNGDFTLCVSIRTAILEGNTINYWAGSGIVYDSDAEK
                     EYQETLLKQKAFLNSIN"
     gene            10483..11304
                     /locus_tag="AC159U_02245"
     CDS             10483..11304
                     /locus_tag="AC159U_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3335906.1"
                     /translation="MSEKIYFYDGSLKESLDILPNRSLFFGEGVFETFRYKSQIPIYI
                     DRHLKRLKKGSEFLQISIPSDDIIIEFIKNSVQEAGLDDAYVKICLFSSGGNSYYDKS
                     QRYILCSVIKEYPHRKSDISLTIATECINSKSELNYYKTINYIQNKLSKRQAISSGFE
                     EALFLNEKGNVTECTAHNIFWIKDSKIYTPSLKNGLLPGITRSVIIDICNDLDLELIE
                     GDYDIKELQRADYIFLTNAISGIVKVYNIEGKEYLEESQIYKNIEDNLLTRLKWK"
     gene            complement(11543..12943)
                     /gene="mnmE"
                     /locus_tag="AC159U_02250"
     CDS             complement(11543..12943)
                     /gene="mnmE"
                     /locus_tag="AC159U_02250"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR3335907.1"
                     /translation="MSKDILNEDTIAAIATAQGHGGIAIIRISGNDATSILKNIYRRS
                     NNKPVEEIKPFRLYRGFIFSDEENRVIDDVLVVYMKAPKSYTGQDVVEIHCHGGVIVS
                     KIILDLCIKRGARAAEPGEFTKRAFLNNKMDLSQAEAVADVINSQTEKALKHSQMQLE
                     GQLSKQINDYKDVVLDMLAEIEANVDFPEEGLDSLVKNRILENSESLIENLEKLISTF
                     EQGHILKHGITTAIIGKPNVGKSSLLNCLLRKDRAIVSEIPGTTRDFIEETIDIYGIP
                     LKLIDTAGIRYTQQDIEQMGIDLSRKISQEAEFQIVVLDRSEKLGSDDLEVLKLLSDS
                     NYIVVLNKNDLGTKISEDDLPSHINRELIIDISAKNNSNIDELRDKIFNIITGDNKSQ
                     EDSSVTISELRHKNSLEQVIENLTRFKKALVIDESPEYLAVDLRYALDKFGEITGEIT
                     SEDVLSRIFSKFCIGK"
     gene            13064..13744
                     /gene="radC"
                     /locus_tag="AC159U_02255"
     CDS             13064..13744
                     /gene="radC"
                     /locus_tag="AC159U_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3335908.1"
                     /translation="MADKPDYLEHRKRIRERFLKSSGKGLSDYELIELLLTYAIPRKD
                     VKPIAKQINRKFGGLKGIIDSTAEELEKIDGIGPASATLILLIKEIIIGYFEYSMVKG
                     DALSSPQIVADFAKAKLSGLKDEVFLCIFVNTKNEVIDYKVIQEGTIDQVVLHPRKVL
                     EGALSMKASGIILIHNHPSGHPQPSEQDKVLTDELKKAAELINLRILDHLIIGKNKYY
                     SFRENSLL"
     gene            complement(13756..14343)
                     /locus_tag="AC159U_02260"
     CDS             complement(13756..14343)
                     /locus_tag="AC159U_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3335909.1"
                     /translation="MSKQEGSSVAGIFWSLSPVVAITSAWDGKVNGQIAVTVVTSSVV
                     HTIPRLIVGIWKGNYTHDFIYNSKKLCIHLLRNDQLALVKNFGFYTGRERKKFKEVAY
                     ETLETGCPVLIDCHSYAECEVLNAMDGGDMTVFLVSVINGGLKSSDQWLTLDYFYTYA
                     PPEWIHEYSHKLSRTVETSLQCIHDIKYQAWEPDK"
     gene            14459..15196
                     /locus_tag="AC159U_02265"
     CDS             14459..15196
                     /locus_tag="AC159U_02265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3335910.1"
                     /translation="MSSLFGTNSEQYNKFRPHYPAELFEYLAGVAPSNELAWDCGCST
                     GQVSVPLSNYFEQVVATDISEGQIKKAEVKDNIIYRASAEGSSGLNKNSVDLVTCAQA
                     LHWFDLKKFYKEVNRVLKPGGVVAVWTYNLFRVNKKLDELIDNFYFDNIYNYWPEQRK
                     DVEKGYAKLEFPYTKISVPQFSMEVEWTLDHLKGYINTWTGVQNYIELECFSPLEFIE
                     GELKNLWGDKKTKKRITWPLMVIVGKK"
     gene            15280..16863
                     /locus_tag="AC159U_02270"
     CDS             15280..16863
                     /locus_tag="AC159U_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR3335911.1"
                     /translation="MMRRFFLLSASILTLAILSYIQQSLAAPPTFSFTSSVIGQGTVE
                     PPTASHKRNWQLTVTAIPADGWVFDHWEGDLSGSENPTRIRITSDKYVTAVFVEAGVP
                     SPTPTPTPIPTVTPTPTPGPSPTPGPSPTPTPTPVPPSSDKEVIGYFIQWGVYARDYF
                     VKNVVTSGSADVLTVINYAFAGIDETLKCESLDPFADYEKAMKVDESIDGVEDTFDQS
                     LRGNFNQLKKLKQMYPNIKLMISIGGWTQSFRFSDAALPQNRQAFVDSCIDMYINGNF
                     APGISEPGLFDGIDIDWEYPGRCGNTCDFREEDTENFTALLQEFRTKLDQVNPDLLLT
                     IASPASEFHYSKIELDKIHQYLDWINIMTYDFHGGWEANGPTNHHSPLYSSPSDPTDG
                     GSTDESVKGYLGQGVPKSKINIGVPFYGRGWARVSNTNNGLYQNAGRVPRGKYEKGVD
                     DYKILSSMGFPGFWDPVAQAYWIYNGNEFWTFDNDQSLLNKMGYIKSNDLRGVFFWEL
                     SGDSADGELIDAINQGLQD"
     gene            complement(16896..17699)
                     /gene="mepA"
                     /locus_tag="AC159U_02275"
     CDS             complement(16896..17699)
                     /gene="mepA"
                     /locus_tag="AC159U_02275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HIR3335912.1"
                     /translation="MSTVSHLLISLVLLSIILFPAEGNSSSKSIGFYTAGCIQNSQKL
                     EETGEGYQVIRLSRERNYGHPRLIDFIKYLGKHTKAKLNSTLLIGDISQKTGGPLPSD
                     HTSHQTGLDADILYLNQPYSNLFLSESEREDINPISVLNAHKTGIKEGMWGRRQTAVL
                     VLATAHPDVDRIFVNPVIKLELCKKHKGQPWLNKIRPWYGHDGHFHVRLKCPNGSNFC
                     ENQKSIEPGDGCGEDLKQWFIPPEKSEEVKTTPEPKKVRQMPEQCLGLL"
     gene            18852..19532
                     /locus_tag="AC159U_02280"
     CDS             18852..19532
                     /locus_tag="AC159U_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335913.1"
                     /translation="MRKFILNLAILFLFSGINSYAGVNVEINLGGSPNDNYYRSLANY
                     YRVPDKDVYFVRDRGIPNEELPVIFYLASLAGVAPSLVADEYRRPGITLYDLTRAFGL
                     YPDIYHYPIKTRVDGPPYGKAYGYYKNKPKNQWKDIKLNNSDIINLVNLRFISESRGV
                     DPDDIIKGRKAGKDFISIDYDISKSKGKPNNASGKKDFDKPKPGKGNSGVEKNKGKGQ
                     DKGKGKKK"
     gene            19547..19786
                     /locus_tag="AC159U_02285"
     CDS             19547..19786
                     /locus_tag="AC159U_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3335914.1"
                     /translation="MKRIKIEQANMYLDTLIDEVTESDEPILIEANGRNSVLVSEQNW
                     RSIQETLYLLSIPKMRESIRDGMAEPLDDCKDKVW"
     gene            19790..>20040
                     /locus_tag="AC159U_02290"
     CDS             19790..>20040
                     /locus_tag="AC159U_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335915.1"
                     /translation="MKDNKYKSSIPKGCIERDIIDKKPDLKDLPEHVAYIASCSQKAL
                     IYKGEMVGIRVYSGDKVLLRETPLKVGKKHGTEYTFYDSG"
BASE COUNT         6882 a         3408 c         3908 g         5842 t
ORIGIN      
        1 ctttatggaa gcgatacaat ggcaggaaaa ctaaactaca gaaaagatga attcaagttt
       61 acatccaagg atgaggaaac aagaaaggcg ctttatatac cggctctttt gctggctgct
      121 ctatttgtac ttgtgcttgt aagaaacagc attaattaca taagggtaaa cagcgagtat
      181 aaaaaactaa gctcacagat agaaaaaagt gtaaaagaag tgttcccaga ggttaaggta
      241 ataccacgcc cggtggactt tatgcaaaat caggtaacaa gagtaaacag tgagcttgat
      301 ctaattgcag gtattaagtc tggcaaaacc ccgcttgata tcatgagaac tctttctaca
      361 acgatcccta ttagtagttc ggttacattt gatgaactta attttactga cgataaaagt
      421 ttaaggctca agggaaaaag taagtcctat gatgatattg caaaagtaga aaaagcacta
      481 aacgactcag ggcattttga aaatgtagtt agaagttcag cgggctctgc gcttgataaa
      541 attaaatttg aaatatcact ggaaataaaa taatggattt ttctaagata aaagaactat
      601 ttgccgggat tagggaaagt atagaaagta atataaatat aagtgaatta acagagcgtg
      661 acaggtatat tatatacggg gtagccgggt ttctagcagt aataattatc tatctagccg
      721 tattttcatt tagctctaaa gttagtaatt tggaaaaaag agtactcaca ttagaaactg
      781 atctacaaag agtagccgat ctcaagtcag agtatattga atcgagcagg cagcttaaaa
      841 atcttgtaaa atcaggtagt aaacaaacag gcccgttaat ttcagtagtt gaaaaggtat
      901 tgttgagtga aagcgttgag agaaagaact tttctataaa ggacagaaac cagcggagca
      961 aggatgctga agacatatat aacgaaaaga gtgttgaggt acaaattaaa cagatccctc
     1021 ttggaaagat gatagatatc ctttatgcgt ttcagtcgaa aaatacgaat ttaaaggtaa
     1081 agggattgag aataagaaca cgatttgata acagtaactc agtagatatt tattttatgg
     1141 tatctacttt tgaatttaag gaagtaagct aaaaatgtct attttaaaaa acaaaaaatt
     1201 gttgtttgta cttacattca tatttgtatt tatatgtttt ttctatattc agtttccaaa
     1261 tgacagactc aaaagaaggc tagaatttga aattgagaaa aatactcagt tcgatgcgca
     1321 gattgataaa ataaaaattt taccgctact aaaaattcaa ataaatggtc ttaaactcag
     1381 tagaaatgat cagcaaatat tgatttccaa agctgtaatt aagccatcca tactatctct
     1441 tctttctaaa gacataatcg tgaagtatga cattaatgtt tttaagggaa gtgctcaagg
     1501 tgatattatt atcacaaaag attccggaca attacaatcc atgattgtta aagttgagag
     1561 tttagacgtc gatgagttta cgcaaatcta ctcacacagt gcaaaagaga acatagaaat
     1621 taaggggcta cttagcggaa atatgaatct tagctcaaat aacacaggtg attttgattt
     1681 ttatatagaa gatttagata tattaaagat taagataaaa acatttgacc ttccgcagtt
     1741 tacggattta agatctaacc ttaaaggaca gatatataga gacagaacca taataaacga
     1801 gcttaaattt gataatgagg acattactct taagcttaaa ggatcaacgc cgcctctatg
     1861 gagactttct aagggaagca ttgacctgct ttacagggtc gatgtaagaa gcaagaaata
     1921 tgcgcttttg aaaagctttt tgaataaaga taataaggga aatgtagcag ggaagatagt
     1981 aggaagctta caaaaaccgc agcttaaaaa tgtaaacgat tcaaatgcac aaccaaaaag
     2041 gcagcagggt ccccggttca gaaagacctt tagccccaag aagcagcagg ctatctaaat
     2101 actttatttt ggcataaaga acacttatat aattttatct taccaaaaga ttcacctatt
     2161 agattttata gatttgacta atgatttgtg tatagctatc attggtttta cttatcttta
     2221 tccatttttg ctatggcgtc caaaatcaaa ttaaaagatt taagggatca aattgatgag
     2281 attgataaaa agcttttatc acttctcaat ctaagggcag gagttgcctt aaaagtaagc
     2341 gaatttaagc agaaaaatgc tgtggacgtt tatgatccgg taagagaaaa acaggtttta
     2401 gaaaaaatta tatccgagaa caaaggccct ctaaattcag atcttgtaaa atcagttttc
     2461 agacagataa tttcagtttg caggtctatc caaaagccta ttgagcttac agtgctcggc
     2521 ccgcccggaa gctattctca tcaagtggcg acaaagattc tgggagtttc ggtagatatc
     2581 aatccggtag ctacgattga agatgttttt gtagatctgc aaaagggtaa aaaccttttt
     2641 ggcgtcgttc ctgtagaaaa ctctgcggag ggatcagttg gaaatgtact tgataaattt
     2701 atagatacgg acattaaaat ctgcacagag cattatgaaa gaataagtca tagccttctt
     2761 tccaaagatg gtaaaattga aaatattaaa acagtggcat cacaccctca ggcgcttgga
     2821 cagtgcagga aatggttatc aaaacgacta aagaacatcg attttaaaga aacatccagt
     2881 actgctaaag cagccgagat tgcatcagaa aataaacata tagcagccat agcaggggaa
     2941 tttaatacct caatatacga tctgcaggta gtagagagca gtatagagga ctatccaaat
     3001 aatactacaa ggttttttct aatagggtat caggagaaca gcccatcggg taatgataaa
     3061 acatcaattg tgttttcact aaaagatgag cccggtgctc ttagcagtgt gcttgagcct
     3121 tttgataaga aaaatataaa ccttactaaa atagagtcca gaccaacaag gactaaaccc
     3181 tgggaatatg tattttttgt cgactttgaa ggtcatagtt cagagaagag catacaaagg
     3241 atcttaaaca acgttagaaa aagctgtgta tttttaaaga tcctaggttc ttatcctaaa
     3301 gggaaagtct agagctagaa gtattgggag aattgtttga aacctaatga atatgtaagg
     3361 gatttagtac cctaccttcc gggaaaacct gttgaggagc tgcaaagaga gcttggtatt
     3421 acaggcgcta taaaacttgc ttcaaacgaa aaccctgttg gagcttcacc taaagcttta
     3481 aaagaagtgg cttcaaaact ctcagatatc aattggtatc cagatggaga ttgctttcat
     3541 ttaaaaaatg aactttcaaa aaagttgggt tttcaaaatg aaaacatagt agtaggaaac
     3601 ggatcaaacg atatcataga aatagcggca aggactttta tgaaacccgg agatgaggct
     3661 gttatgggtg agtatgcttt tatagtttat ccgcttgtca caaaagctat aggtgctgat
     3721 gcggtaatct caccaatgcc tgatcttata catgatctaa gggatatgta taaaagaatc
     3781 actgataaaa caaaaatgat attgatagct aatcccaaca accctaccgg tactatggta
     3841 aatagggatg aaatggagtg gtttttacaa aaagttcctg acgacgtatt ggtccttgta
     3901 gacgaagcat attttgaata tgtaaatgat aaaaattacc caaatacttt agaatatctc
     3961 agtaacagac catcgcttat caccgttaga actttttcaa agatttacgg cctagcagga
     4021 ctcaggcttg gatatggcat atcatcagag cagataatat cttatatgaa cagggtaaga
     4081 gagccgttta atgtaaattc ccttgcacag agcgcgggtg ttgctgcatt aaatgacaaa
     4141 gagcatgtag ataattccaa taagatcaat agtgaaggca ggaagtattt gataaaggag
     4201 ttagaaaaaa taggtattga atattatgac tcatatacca attttatatt aatagattta
     4261 aaaagagatc caatggctgt gtataatgag ctattaaaac taggagttat agtaaggccg
     4321 gttgggggtt atggactgaa aacccatctt cgtgtaacta taggtctatt agaacaaaac
     4381 ataagattta ttaagagcct taagaaagta ttggattaat acttataaat atgcaattta
     4441 ataaaattat aataatagga cttgggctaa ttggagggtc acttgcactc gcgttaaaaa
     4501 aatcaggtca ggtaaaacat gtagtaggag tggacgtgct accggagtct gtagaaatcg
     4561 ccttaagaaa aggaattatc gatgagggac aggtaggtat tgatgaagta gtgtatgatt
     4621 ctgatatagt agtaatagct actcatgtag gaagcattgt agatattgca agagcaataa
     4681 gtgtttcgga ttctacggtt gttaccgata caggaagcgt aaaggaaaat atagttaatg
     4741 aaatagaaaa tagctgtaat ttaagattta taggatccca tcccatagcg ggtacggaaa
     4801 aatcaggtgt tagtaatgtg gtagatgggc tttttaaaga caaaataaca ataatcaccc
     4861 caacaaataa tagtacatcc gaagctacag agacggtaac gcggatgtgg actgtcatac
     4921 aaagtaaggt catacgcttg agtgcgcaaa aacatgatga gatttttgca cacgtaagcc
     4981 atttgcccca cgtttgtgca tttagcctgg taaattcact tattaaaaaa gacagcacat
     5041 tatttaatta tggcggtgga ggtcttaagg acttttcaag aatcgctgag agctcacctg
     5101 atatgtgggt tgatatattt atctataata aggatgagat attaaaagct attgatgaat
     5161 ataaagatag tctgagtgag attgaaaatt acataaaaaa aagtgataaa aagtcactta
     5221 aaaatatatt gggtagttct tctaatataa aaaaatagta ataataaaat tatttatact
     5281 taaaaaatat tatttattga atattaaaaa actttagtta atatttcagg attatgaaaa
     5341 ttctattaag cgtaattata gcactagcag ttggaacaat agcacttaat gcatggatat
     5401 taagtgaggt atcaaatata aaagcatcgg ttgccaagca ggatgaagaa atagctaaac
     5461 tcagtaaagc aatagccgca agaccagcag cacctgccgc acaacctgca gcagcagatc
     5521 cacagaatgt taaggtgagt atagatgacg atcctattaa aggtgatttg aaagcacctg
     5581 taacaatagt ggaatttagc gattatgagt gtcctttctg taaaagaaat cacgatcagg
     5641 ttttttcaca gataaaaaaa gaatacatag acactggtaa ggtaagatat gtattcaggg
     5701 attacccact tggtttccat aaaaaggcaa tacctgcagc aatcgccgca aactgtgccg
     5761 gagaacaggg taagtactgg gaaataaacg actttttatt tcaaagtaaa gcaaatttat
     5821 ccgatgaaaa atatatagaa tttgctaaaa acaacaacct tgacatggaa aagtaccagg
     5881 catgccttaa agacaaaaag caagaggctg aagtagataa agattttaaa gacggccagg
     5941 cttatggagt tagaggcaca ccaagtttct ttattggtaa aactcaagaa ggtaaagaaa
     6001 tgacaggcgc ttacattaga ggtgcaagac cttttgatgc ttttaaaacc gagattgata
     6061 aaattctttc tgctcaaaaa taatattaca tctaacaaat aaaatattgt ctctggcttt
     6121 ttaagccaga gacaatttct tttattaata aagcatatca cattttatat taatatagct
     6181 ttgtctgtac tgcttattaa tcatttacta tttatccctc cagtttgaca aatatatcta
     6241 atagtattaa tatttaataa ataaatatta atgattgcag cattttaaac tcgaaagtta
     6301 aagagaaata tatataaatt atgcagagga tgatgaactc tcgttattta gtaagcttga
     6361 acaactattt atgtctaatt gtattcattt tcttatttag ttttattatt accgagaatt
     6421 cttacagcca gtcattctac tataaaaaac aaggtaatgt tgtggtttat acaaacctaa
     6481 cacctgactc tggagggttt aaaaaactaa ataccccctg ggggaaaaca cagaaacaga
     6541 aaattaaata tggatctaca aaatactcag atggttacaa taaagaaata ttaaaagcat
     6601 caagacaata ttcccttgat ccaaacataa taaaagcaat tatcaagatt gaatcaaatt
     6661 tcgacccaaa agcagtttcc cgtaaggggg caatgggctt aatgcagctt atgcccggga
     6721 cagcacttgg tcttggtgta tcaaatcctt tcgacccggg acagaatatc aggggaggaa
     6781 caaaatattt taaaaaactt atgaatatgt ttaacggcaa tattaagctt gctcttgcag
     6841 gatataatgc cggtgaaaat gccgttataa aatatggata ttcaattcct ccgtacaatg
     6901 aaaccgagaa ttatgtagaa aaggttttat atcattacaa caatctaaaa gggaaaaaca
     6961 gctataacta taatacaggg cagacaagct ctttggtagt taaaacttct gcaagaagca
     7021 acaattataa aaacaccgat gtatttgatg aggagcagct aagaggtgaa ctatccggta
     7081 aatttcttat tcaacttgca tcctatccta agcttggcct tgcaaaagaa cttgaaaaca
     7141 ctttaaaatc caagggttat cctgcattta ttcaaaaagc ctaccttcca aacagcggta
     7201 cctggtacag agtaagaata ggctctttta caacaaaaca cgaagcccag cagttcaaaa
     7261 attcgctaaa atcaacagag ccattcataa atgatgcaat agtggtaaat ctttaagcca
     7321 gttattttaa tatacagccc tatctagcct ataacactta acaaattctt gactaaagcc
     7381 ctgatttata taaaatagca tagcctctag tgggtacgtt tttcaaaatt tacattggaa
     7441 ggggtaatta tgtcaaacaa gccatcagaa tatacaccgc cagtacaaga taatgaattt
     7501 attacaacta taaccgataa accagaggat cttttagagg taggagttct gtttataggt
     7561 gcagggcctg caagtcttgc aggtgccata agactggcac agcttcttga aaatgagcca
     7621 gaaattatgg aatcacttgg agaaatacca atcgcagtac tggaaaaggg taaatacccg
     7681 ggcgctcatc ttgtttcagg tgctgtagta aatccaatag caataagaag attattcccc
     7741 gaactaaagg atagcgattt tcctttcctg caggaagtaa atggtgagtc tttatatttt
     7801 atgactgaaa atatgcgtat tcccctaccc acacctccaa caatgaaaaa ccatggaaac
     7861 tttgtggctt ccataagcca gcttgccgca tggcttggag aaaaggctga agaactggga
     7921 gtcatggttt ttaatgaaat gtcggcagta aagctcatag tagaagacaa tgttgtaaaa
     7981 ggcgtaagaa ccgatgataa aggggccgac aaggatggca atcctcttga aaatttccag
     8041 ccgggctcag acattcttgc caaggctacg gttttaggag aaggcacaac cggccatcta
     8101 tcgatggcat tattcgatca ctttgaaatt ccaagagaaa acccccagat ttatgcactt
     8161 ggtgtaaaag aggtctggga agttgcaaaa ccacttggag aggttataca cacgatggga
     8221 tggcctctta gtacttcatc aaaaaataac gaatttggcg gcagtttcat atatcctatg
     8281 ggtgacgaca aagtatgtat aggacttgta gtagggcttg attataaaga cgcctcacta
     8341 tcggttcatg acctgctgca gcagttaaaa agccatccga tggtcaaaaa gatactcgag
     8401 ggcggcaaaa aagcagacaa agggtgggga gcaaagacaa ttccagaagg cggctactat
     8461 gcagtacccg ataaactaaa cgttccagga gccgtagtag tgggagattc agctgggctt
     8521 gttaatgtgc ctgcgctaaa aggaattcac tatgcaatga tgtcaggcat gcttgctgct
     8581 gaatccatct ttaactctct taaacaaaaa aacgatttac agacatattc aggtctcgat
     8641 tcctatgatg cagctgtaag agagagcttt ataatgaaag atctctatga agtaagaaac
     8701 atgagacagg cctttcagta tggcttcata ccaggcttta ttttgtccgg tctaatgacc
     8761 gttaccaagg gtaaatttcc gggcggaaag tttacaggcc atcatgacag cgaacaggaa
     8821 atgttcattg ggcagaggga ttatccaaaa cctgataatg aatactattt tgataagctt
     8881 acaagtgtat ttgacagtgg taacagaaca agagacaatc agcaggatca tgtcaggatt
     8941 gaaaaagagg tatctgaaga agtcggtgag gcatggataa acatgtgccc ggcgcaggtt
     9001 tatgaatggc atgaagaagg cggcaagaaa gtaataagaa ctgaccccac aaactgtatt
     9061 cagtgcggtg ctattacttc aaagggtgga agacttactc caccagaagg cggtagcggc
     9121 cctgaataca ctcaaactta aaaactttat ttcttcctta ataaaatatg agtagttaat
     9181 acagaaagcc cttattttag ggcttctgta ttcttttcta gatttatttc ttttaataat
     9241 tatcttaaat ctatggattt gaatgaaatt tataataacg catctgccat attactcagc
     9301 tctcaaaatt gctggataga tgagagttac ttatacgttt tttcaggcaa acccgaattc
     9361 accttatatc taactgataa taaccttttt cttggtaaaa ataatgaaat tacagaactt
     9421 tttggtgtaa acccttttga ttttattgaa caacaagcag caaatggtaa atatgcggcc
     9481 ggttatataa gttataatta tttggaatac acagacatag gcttaactct cagaaacaag
     9541 aaatttaatg ataatttccc gcttctatat ttcaattttt atagtcctgc caaacttgat
     9601 aaattcaata ttaaacaagc tgagaagatt cttaatatca aacaaaaaga aattagatcc
     9661 aaacaatctg atattcaata ttctacgcat aaagaaggat atatattaga tataaacaag
     9721 ataaaagaat atattgaaaa cggagatgta tatcaggtta acttgtctca taaaataacg
     9781 acccgattac aaaataatcc actaaatact ttttttaact attataaagc tcaacccgta
     9841 ccttatggaa tatatataga ctgtgataag tttcatttcc tgggaggctc catggagcag
     9901 tttgtagaaa aaaggggaga tataattaca acaaaaccca ttaaaggtac ttctaaaaga
     9961 aatcagcata aatcaaaaga tgatcaaata aaggccaatt taagcacaaa tccgaaagaa
    10021 aaagcagaaa atctgatgat agtagatctt atgagaaatg atttatcgag ggtttgcaaa
    10081 ccagcaacag tgaaagtaaa caagctcttc aatgtaaaat catacagaac tctccatcag
    10141 atggaatctg agattaccgg gcagctacta caagataaaa ggttaaaaga tattatatat
    10201 agtgtatttc caccaggctc tgtaacagga gcgcccaaga aaagatcaat acagatcata
    10261 gatgagctcg aaaaccatag caggggcccg tattgcggct gtgcgggtgt gtttagtcca
    10321 aatggagact ttaccctttg cgtatcgata agaactgcta tattagaggg taatactata
    10381 aattactggg ccggaagcgg tatagtatat gattctgatg ctgaaaaaga atatcaggaa
    10441 acattattaa aacaaaaagc atttttaaat tctataaatt aaatgtctga gaaaatatat
    10501 ttttatgacg gctctcttaa agagagtttg gatatcctgc ctaacaggtc tttatttttc
    10561 ggagagggtg tatttgagac atttagatat aaatctcaaa tacccattta tattgacagg
    10621 catttaaaaa gactaaaaaa gggctcagag tttctacaaa tatcaattcc atctgatgat
    10681 attataatag aatttataaa aaattccgta caagaggcag ggttagacga cgcctatgta
    10741 aaaatatgcc tgttttcatc cggtggtaat agctattacg ataaatcaca aaggtatata
    10801 ctttgttctg taattaaaga atatcctcat agaaaaagtg atataagcct aacgatagct
    10861 acagagtgta taaactcaaa atcagaacta aattactata aaacaataaa ctatatccaa
    10921 aacaaactat caaaaaggca ggctattagt tcaggatttg aggaggcttt gtttctaaat
    10981 gaaaagggaa atgtcacaga atgtacagca cataacatat tttggataaa agacagcaaa
    11041 atttatactc cttcactcaa aaatgggtta ttaccaggta ttacaagaag tgtaattata
    11101 gacatatgta atgacctaga tttagaactc atagaagggg attatgatat aaaagaactt
    11161 caaagagccg attatatatt cttaacaaac gctatttcag ggattgttaa agtatataat
    11221 atagaaggaa aagagtatct cgaagaatca caaatttaca aaaatattga agacaattta
    11281 ttaacaagac tcaagtggaa atgatgccca aaaacttaat aaattttata atatattttg
    11341 ttaacctcct gatttgactg atttttttaa ataaaaatta ataaatcata tctgtggatc
    11401 gattgtggat aaaatattac atataatttc aatgttgtcg taactacctg taatcattag
    11461 atatacctat taatacaggc ttaatagaga tacttaattt tatccacacg gcaagtgttt
    11521 cacgtgaaac gtagagtaag aactattttc caatacaaaa cttactgaat attctggaca
    11581 gcacatcttc agaggttatt tcacctgtaa tctcaccaaa tttgtccagt gcatatctta
    11641 aatccaccgc cagatattcc ggtgattcat caatcaccaa agctttttta aatctcgtta
    11701 aattttcaat tacctgttct aatgagtttt tatggcgaag ctctgatatt gtaacagagg
    11761 agtcttcttg agacttatta tcccctgtaa taatgttaaa aattttatct ctaagttcat
    11821 caatattact attattcttt gcagaaatat cgattatcag ctctctattg atatgagaag
    11881 gaaggtcgtc ttcagaaatt ttagttccta aatcattttt atttagtaca acaatatagt
    11941 ttgaatcact caatagctta agtacttcca gatcatcact accaagtttt tcacttctat
    12001 cgagtaccac tatctgaaat tcagcttctt gagatatttt tctgctcaga tcaataccca
    12061 tctgctcaat atcttgctga gtgtatcgga ttccggcagt atcaatcagt tttaggggta
    12121 ttccatagat atctattgtt tcttcaataa agtcccttgt tgtacctgga atttcgctta
    12181 caatcgctct atcctttctt aggaggcagt ttagaagact cgattttcca acattgggct
    12241 ttcctataat cgcagtagta atgccatgtt ttagtatatg accttgttca aaggtggata
    12301 tcagtttttc cagattttcg attaatgatt cagaattctc caaaatccta ttttttacca
    12361 gtgaatcaag gccttcttca ggaaaatcaa catttgcctc aatttcagcc agcatatcta
    12421 atacaacgtc cttataatca ttaatttgtt tagataactg tccttcgagc tgcatttggg
    12481 agtgcttcag tgctttttca gtctgtgagt taattacatc ggcaacagct tctgcctgag
    12541 ataggtccat tttattgttt aaaaatgctc tttttgtaaa ttctcccggt tccgctgctc
    12601 tagctcccct tttaatacat agatccaaaa tgattttaga tactatcact cctccatgac
    12661 aatgaatttc cactacatcc tgacccgtgt atgacttggg tgctttcata tacactacta
    12721 aaacatcatc aataaccctg ttttcttcat cgctgaatat aaaccctcta tagagcctaa
    12781 agggcttaat ttcttctaca ggtttattat ttgatctcct gtatatattt tttaatatag
    12841 aagtagcatc gtttccgctt attctaatta ttgcaatacc gccatgtccc tgtgctgtag
    12901 caattgcggc tattgtgtct tcatttaata tatctttgct caaatttgta tcctttttac
    12961 gtataaaagt atataatttt acctgtttta ttgataactg cttgaattta gtgaaaaagt
    13021 gttaagaaat tatgtttcac gtgaaacatt gggttaattg gaaatggcag ataagccgga
    13081 ctatttagag catagaaagc gtattagaga gagattccta aaatcctctg gaaaagggct
    13141 tagtgactat gagctcatag aactgcttct tacttatgca attccaagaa aagatgtaaa
    13201 accgattgcc aagcagataa atagaaaatt tggcggactt aaaggtatta tagacagcac
    13261 tgccgaagaa ttggagaaaa tagatggaat aggcccggct tcggctactc taattttatt
    13321 gatcaaagaa attatcatag gttatttcga atacagtatg gttaagggtg atgcacttag
    13381 ctctcctcaa attgtagcag attttgcaaa ggcaaagctt tcgggtctta aagacgaggt
    13441 atttttatgc atattcgtaa ataccaagaa tgaagtgata gattacaagg ttattcagga
    13501 gggcacgatt gatcaggtgg tcctccatcc aagaaaagta ttagaagggg cactaagcat
    13561 gaaagcatcc ggtattatac tgattcataa ccatccaagt ggacacccgc aaccctcgga
    13621 gcaggataaa gttcttacag atgaattgaa aaaggcagca gagcttatta atttaagaat
    13681 tctggatcat ttgattatag gtaaaaataa atattacagc ttcagggaaa acagcctgtt
    13741 ataatttcat tccttttatt tatctggttc ccaggcctga tatttaatat catgtatgca
    13801 ctgaagcgaa gtttccacgg tgcgtgagag tttatggctg tattcgtgaa tccactcagg
    13861 cggggcatat gtatagaagt aatccagggt aagccactgg tcgctgcttt tgagaccgcc
    13921 atttatcaca cttactagaa acacggtcat atcgcctccg tccattgcat ttaatacttc
    13981 acattccgca taagaatggc agtcaataag aacagggcat ccggtttcta aagtctcgta
    14041 tgccacctct ttaaacttct tcctttctct acctgtatag aaaccgaagt tttttacaag
    14101 ggcaagctgg tcgtttctca gtaaatgaat acaaagcttt ttggagttat agataaaatc
    14161 gtgcgtatag tttcctttcc atattcctac aataagacgc ggtattgtat gaaccacaga
    14221 tgatgtgact acagttacag cgatctgccc gtttactttt ccatcccacg cacttgtgat
    14281 tgccaccacg ggagagagtg accaaaagat tcctgctact gacgaccctt cctgtttgct
    14341 cattatctta tcataattta tttaagcact aatttgcaag taaaatattt tatcattatt
    14401 attagatcag tgagacattg aggctgtata cgtcctgtcc ccatatatta tattaattat
    14461 gtctagtcta ttcggaacta attcagagca atataataaa ttcagaccac attatcctgc
    14521 tgagctgttt gaataccttg caggcgttgc accatcaaac gagttagcct gggactgcgg
    14581 ctgcagcacc gggcaggttt cggttccact ttctaactat tttgagcagg tagtagctac
    14641 ggatattagt gaagggcaga taaaaaaagc cgaggtaaaa gacaatatta tttatagggc
    14701 cagtgctgaa ggcagttcag gattaaataa aaattctgtt gatcttgtaa catgcgcaca
    14761 ggctctgcac tggtttgatt taaaaaagtt ctataaagag gtaaacaggg tgcttaagcc
    14821 cggcggggta gtagcggtgt ggacatacaa cctctttaga gtaaataaaa agttagatga
    14881 actaatagat aatttttatt ttgataacat ctataactac tggcccgagc aaaggaagga
    14941 tgtggaaaag ggctatgcga agcttgagtt tccgtacact aaaatttctg tcccgcagtt
    15001 ttcaatggag gtagagtgga cattggatca cttaaaaggc tacataaaca catggaccgg
    15061 ggtacagaac tatattgagc ttgagtgttt cagcccgctt gaatttattg agggagaact
    15121 gaaaaacctc tggggagata agaaaacaaa aaagaggatt acctggccgc ttatggtgat
    15181 agtaggtaaa aaataataag gcgaagtctc aattgacttt gaattactac agtaatacaa
    15241 tgtaattata tctcagaata tttataagat gggggttata tgatgagaag attttttctg
    15301 ttatctgctt caattttaac tttagcaatt ctttcttata tacagcaaag ccttgcagct
    15361 cctccgacat tttcatttac ttcatcagtt ataggacaag gaacagtaga acctccgact
    15421 gcctcacata aaagaaactg gcagcttacc gttactgcaa tacccgctga tggatgggtg
    15481 ttcgatcact gggaaggtga tttaagcgga agcgaaaacc ccacaaggat tagaattaca
    15541 tcagataaat atgtaacagc ggtatttgta gaagccggag ttccttcccc tacaccgaca
    15601 cccacaccaa ttccaacagt gactcctacc ccaactcctg gtccatcacc tacacccggt
    15661 ccttctccaa cacccacacc aacgccagtg cctccttcat cggataaaga agtaataggc
    15721 tacttcatcc agtggggcgt ttatgcaagg gactattttg ttaagaatgt ggtcactagc
    15781 ggctcggctg atgttctcac ggttattaac tatgcatttg caggaataga tgaaactctc
    15841 aaatgtgaaa gcctcgatcc atttgctgac tacgaaaagg ctatgaaggt ggatgagagc
    15901 atcgatggcg tggaagacac atttgatcag agcctcaggg gaaattttaa tcagcttaaa
    15961 aaactaaagc agatgtatcc gaatataaag cttatgatat caattggagg ctggacacag
    16021 tcattccgct tttctgatgc tgctctgccg cagaacaggc aggcatttgt agattcatgt
    16081 atagatatgt atataaacgg caattttgcc cccgggattt ccgagcccgg gctttttgac
    16141 ggtatcgaca tagactggga gtatcccgga agatgcggca acacatgtga ttttagagaa
    16201 gaagacacag agaattttac agcgcttctc caagaattca gaacaaagct tgatcaggtt
    16261 aatcccgatc tgcttctaac catcgcatca cctgcctctg agtttcacta ctccaaaatc
    16321 gagcttgata agattcatca gtacctggac tggatcaata tcatgaccta tgattttcac
    16381 ggaggctggg aggcaaacgg acccacaaac catcactctc ctttatacag ctctccgtct
    16441 gatccaacag acgggggttc tacagatgag agtgtaaagg gttatcttgg acagggcgtt
    16501 ccaaagagca agataaatat aggagtacct ttttatggac gcggctgggc aagggtttct
    16561 aatacaaaca atggcctata ccagaatgcc ggcagggttc cccgcggaaa gtatgaaaag
    16621 ggtgtggatg actacaagat tctaagctct atgggatttc cggggttttg ggacccggtg
    16681 gctcaggctt actggatcta taacggaaac gagttctgga cttttgacaa cgatcagtcg
    16741 cttctaaaca agatgggcta tatcaaatca aacgatctaa gaggggtatt tttctgggag
    16801 cttagtggag acagcgcgga tggtgaactt atagacgcaa ttaatcaggg acttcaggat
    16861 taatttttca cactatcatt cggggctggt tgagttcaaa gtagtccaag acactgctcc
    16921 ggcatctgcc tgactttttt tggttcggga gtggttttta cttcttcact tttttcaggc
    16981 ggtatgaacc actgcttcag atcctctccg cagccgtcac cgggttctat agatttctga
    17041 ttttcacaga aattgcttcc attcggacat tttagtctta catgaaagtg gccgtcatga
    17101 ccataccagg ggcggatttt atttagccat ggttgtccct tgtgtttttt gcataactcc
    17161 agtttaataa ccgggtttac gaaaatcctg tctacatcgg ggtgagcagt agcaaggacg
    17221 agcacggccg tctgccttct tccccacatc ccttctttaa tgcctgtttt atgagcgttt
    17281 aataccgaaa taggattaat gtcttcgcgt tcggattcac ttaaaaatag gtttgaataa
    17341 ggctggttta ggtacaggat gtcagcatca agacccgtct gatggcttgt atggtcgctt
    17401 ggtagggggc ctccggtttt ttgagaaata tcaccaatta acagagttga atttaattta
    17461 gctttagtat gtttgcccag atacttgatg aaatctatca gtcgtgggtg cccatagttt
    17521 ctttccctgc taagccttat aacctgatat ccctcgcccg tctcttcaag cttttgagaa
    17581 ttttgtatac agccggctgt ataaaaccca atggatttgg aactggaatt accctcagca
    17641 ggaaataaga ttattgataa gagaactaaa gatattaata gatgcgaaac tgtactcatg
    17701 tttacccata tatgataatt aaaactaata tattagcaaa aagctttgta acacactgag
    17761 tctaccaaag tgtgagttgc aacaatttag ttctagacta agttaatttc ctgataatta
    17821 ttctccatta ttgtacttac atacagtatg tatgttgaat attcatatct tctcttctga
    17881 tcaagaatag ttctaaaggg gaacattcaa ttaaaataaa tctatgcaaa tatatacata
    17941 ccgactgtcg gtatgtataa aaatgacaca atggaagagt aataactgtt aatttttagt
    18001 gcgtatttga tgctatgaaa cggtaaacag tcaaacggtc aaaagttcag ggtatagccc
    18061 cttgtctgat gactttttga ccgtaagtac ttgtaatgtc taaaattcac ctgtttgggt
    18121 cttacggtca atggtcagcc actagaccca aacccattga cccaaagacc ctaactgaga
    18181 caaaaaatta agatggtaat atcaattttt aataattaca ggcagttaca acagttagac
    18241 ccaaaaaccc aaagattctc ctataagccc ctaaacctat gggtttttgg gtttaaggct
    18301 aattagtcag taattacatt taattagaag cagattttat attttcaact aagagtgatt
    18361 aatgaatact tatttagata accagttgta tatttattgt tatttccagg tgaatttctt
    18421 ttttaatata attagtatta atggatagga taaagagcat aataaataat taaaatgtat
    18481 ataaatagat aatatataaa ttagtataat aattacagac acttacaact cttagaccca
    18541 aaaacccaaa agtttcgggt ttatgccctg atttatgggt ttttgggttt aactggttac
    18601 caatatagtt agatatgagt atataagtgc ggagataggc aggatattac ttccaattac
    18661 cgcactaagg gtcaaaaggt taaaggtttc agggtatatt aataccctaa tgacccgaac
    18721 taacttttag ggttaaataa gctttactag aaatttggat aacaacatta aattgaaaag
    18781 tagtacagga ctaaagctat gaaactaaaa gcgctctatt tagtttttta ttctataaag
    18841 ggaggatgat tatgaggaaa tttatattaa atctagcaat cttattttta tttagtggca
    18901 taaatagtta tgccggagtg aatgtagaaa ttaaccttgg cggaagccca aatgataatt
    18961 actacaggtc acttgcaaat tattacaggg ttcctgacaa agatgtctat tttgtaagag
    19021 acaggggaat tccaaatgaa gaactgcccg tgatattcta ccttgcctca cttgcgggtg
    19081 tagctccctc tttagtagcc gatgaataca ggaggccggg gattacgctt tatgatctca
    19141 caagagcttt tggactatat cccgatattt atcattatcc gataaagacc agagtagacg
    19201 gtccgcctta tggtaaggct tacggttact acaagaacaa acccaaaaat cagtggaagg
    19261 atattaagct caacaactca gacatcatta atcttgtaaa cctgaggttt atctcagagt
    19321 ccagaggagt cgatcccgat gatataataa aaggaagaaa agccggcaag gatttcattt
    19381 ccatagacta cgatatttcc aagagcaaag gaaaacccaa taatgcatcc ggcaaaaagg
    19441 atttcgataa gcccaaaccg ggtaagggta attctggagt agaaaagaat aagggtaaag
    19501 gacaggacaa aggtaagggg aagaagaaat aagtaggtag atctccatga agagaataaa
    19561 aattgaacaa gcaaacatgt atttggatac tctaatagat gaagtgacag aatcggatga
    19621 accaatattg attgaagcta atgggagaaa cagtgtttta gtatccgaac aaaactggag
    19681 atcaattcaa gaaacgttgt atctcttgtc tattccaaaa atgagagaat ctattaggga
    19741 tgggatggct gagccgttag atgattgtaa agataaagtt tggtagttaa tgaaagacaa
    19801 taaatacaaa tcctcaatcc ccaaaggctg tatcgaacga gatattattg ataaaaagcc
    19861 tgatcttaaa gacctgccag agcatgttgc ttatatagca tcctgtagtc aaaaagctct
    19921 catatataaa ggagaaatgg tcggaatcag ggtttacagc ggggataaag tacttttgag
    19981 agaaacgccg cttaaagtag gaaagaaaca cggcaccgaa tatacatttt atgattcagg
//
LOCUS       DBNGSV010000059         7876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_150369, whole genome shotgun sequence.
ACCESSION   DBNGSV010000059 DBNGSV010000000
VERSION     DBNGSV010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_150369"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC159U_02295"
     CDS             complement(<1..189)
                     /locus_tag="AC159U_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335916.1"
                     /translation="MARCINTISVLTLAVVITLFISVSLFAEDNSAPLDNSLIEKGKN
                     VYDSNGCSACHTLGGGKLA"
     gene            944..1546
                     /locus_tag="AC159U_02300"
     CDS             944..1546
                     /locus_tag="AC159U_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3335917.1"
                     /translation="MNVNNYILILFLVLLILPLSCKDEIPVKGSFPNDISLVDHTGKE
                     FLFSDLKGKVLLVSYIYTNCPDICHIINKKMNVFKSHIARDSISNDVVFVSISIDPQN
                     DTPEKLRKHIHHMNFDTSNWYFVTGNIGSVYRLIANAGIFPVRESKQNDADNENAYLN
                     YIISHRDRISLVDKNGNIRKHYKGTTMDSDIILMDMKSLI"
     gene            1700..2038
                     /locus_tag="AC159U_02305"
     CDS             1700..2038
                     /locus_tag="AC159U_02305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3335918.1"
                     /translation="MKERRAYPRVEIREDVMFGVNKPARSGKSENLSPGGMCIISDSY
                     LPQGSDIVIDISVKHTITDGSETWENITVYGKVAWVDSPPGNYSKMGIEFSSTNEKLH
                     MIYEHKKSEI"
     gene            2158..2649
                     /locus_tag="AC159U_02310"
     CDS             2158..2649
                     /locus_tag="AC159U_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3335919.1"
                     /translation="MSELNDSMREFLAGCHYATLATLNDDGSVHQTPVWYLFKDGNFY
                     LTTGSSGRKARNLLNRSEATILVDSRRNQGHERWVSAVGTAEIIKGEKSKEINSKIIS
                     RYLTKEGMDDPRVAGVFEASGDATICITPDSWKAFEFSTVDKQFFGGILGETPDKWFN
                     VVD"
     gene            complement(2703..4163)
                     /locus_tag="AC159U_02315"
     CDS             complement(2703..4163)
                     /locus_tag="AC159U_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR3335920.1"
                     /translation="MSGASKFGSKTGIAVIVVTVALLTAGITALLLNIFERKVEARNP
                     FVRLVEVTETDTDPEKWKVNWPKQYDSYKKTSVATRTRFGGHGGSEALPEQKIERDPW
                     LKRMFKGYAFSIDYRDRRGHAYMLEDQEQTERQTKPQSGSCLHCHASIMPLYEKLGNG
                     DVIKGFEQTYKYSYKDLNKQLHDMGHGNPVSCVDCHSPKTMELRVTRPGFIQGIQALA
                     ESDAPTPHLPSIEQWRKGSKSEPYNPNTDASRSEMRSYVCGQCHVEYYCGADMKLTFP
                     WGNGLKVEDQEKFWDETTLSDGRKFFDYTHKETGAKILKAQHPEFELWSQGIHARSGV
                     GCADCHMPYMRDGATKVSDHWVRSPLLNINRACQTCHRFSEQEIKERVDGIQQRNFDL
                     LQRAGASLMELLDAINTARAEGATDVQLKKALDMQRKAQWRLDFIAAENSMGFHAPQE
                     AARILGESIDYAWQGTVAATKWNKVNVASNVADESK"
     gene            complement(4163..4660)
                     /gene="nrfH"
                     /locus_tag="AC159U_02320"
     CDS             complement(4163..4660)
                     /gene="nrfH"
                     /locus_tag="AC159U_02320"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HIR3335921.1"
                     /translation="MKKRAIILSIAVGALIGLGSYTMYYGEGLSYLSKDPRSCVNCHI
                     MQSQYDSWQKASHHTAATCVQCHLPHDFVNKYISKAVNGYHHSKAFTLQNFHEPIMIT
                     EKNSIILQNNCIDCHRGITENLTHGSTATPDAIKCVKCHKAVGHGETTGLGGPIKSHE
                     TGGIK"
     gene            4908..5141
                     /locus_tag="AC159U_02325"
     CDS             4908..5141
                     /locus_tag="AC159U_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335922.1"
                     /translation="MTKKLPEYDHMVEIKEDEKKIYVFRIYKKSKKKQLFTCYELPAK
                     SFDEDKKAFQEFAKILGENLLIDSPTARKLFGI"
     gene            complement(5160..5531)
                     /gene="rplS"
                     /locus_tag="AC159U_02330"
     CDS             complement(5160..5531)
                     /gene="rplS"
                     /locus_tag="AC159U_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011065937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3335923.1"
                     /translation="MGIVSEIEKPLLRENAEEFRPGDTVAVHYKIKEGDRERIQIFEG
                     VVIAKKNGGLRETFTVRKISYGIGIERIIPLHSPLIEKIEVKRLGKVRRAKLYYLRGL
                     SRKAARIPERTKPDMVKKKKG"
     gene            complement(5547..6845)
                     /gene="trmD"
                     /locus_tag="AC159U_02335"
     CDS             complement(5547..6845)
                     /gene="trmD"
                     /locus_tag="AC159U_02335"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3335924.1"
                     /translation="MQFNILTIFPEMFEGVLSSGVIGRAADSGLLNINTYNIRDYTTD
                     KHRMVDDRPYGGGSGMVMKPEPIAAALKDVTNSDDKSKVILLSPGGKTFNNEIAKDLA
                     DYETLIFICGRYEGVDERIKEKYVDMELSIGDYVLSGGEYAAMAIIDSVARYIPGVLG
                     NDQSTVDESFSSGLLEYPQYTRPEEFEGSKVPEVLLSGDHGKIGDWRRQKSIEKTFAS
                     RPDLIDRNILELDDINTVDNLKRKLEKNSNLYIALVHYPVYNKRLDVVKTSFTNIDVL
                     DMARAGRTYGVKGFYLVHPVAEQRDLIRTVMEHWTKGKGYEYNTSRKESLEYTYISES
                     LSDTIETIEKLEGSKPKIIVTDARYSDKMTGYLEMRETIESDSNPFLLLFGTGYGLIR
                     EIIEGSDYNLKPVVGSEIFNHLSVRSAASIILDRLCSVKI"
     gene            complement(6933..7448)
                     /gene="rimM"
                     /locus_tag="AC159U_02340"
     CDS             complement(6933..7448)
                     /gene="rimM"
                     /locus_tag="AC159U_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3335925.1"
                     /translation="MGFIPYGVISKSHGLAGGVRLIIYSGDTSNLDFIESLYIQDGDT
                     EEFTKYNIEGHSLSGKHAVIRLQGVNSIEQADKLKGTEVFVDDSQIEPAGGDEFYYYE
                     LEGLEVFDTNNNLLGKVIRLNDSGPQELLEAQLTSGKEVLIPFIEPILVEVDIDNKKI
                     VVDPPPGLLDL"
     gene            complement(7470..7709)
                     /locus_tag="AC159U_02345"
     CDS             complement(7470..7709)
                     /locus_tag="AC159U_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3335926.1"
                     /translation="MEKHTELISYIAKSLVDSPDQVEVKEVIGEQTTVLELKVAQEDL
                     GKIIGKQGKTAKAIRTILGAAAAKAKKRAVLEILE"
BASE COUNT         2238 a         1608 c         1454 g         2576 t
ORIGIN      
        1 agcgagtttg ccgcctccta aggtgtgaca ggcgctgcaa ccattgctgt catatacatt
       61 ttttcctttt tcgataagag aattatccaa gggagcagaa ttatcttcag caaataacga
      121 gactgagata aatagtgtaa tgacgactgc taaggtaagt actgaaattg tgtttataca
      181 tcttgccatt atctttcgca tctccttgtg tgaatgtttc aagattttta tctacacagt
      241 aataattaat gcaatagata tgccaaggac aaatcggcgt gtttataagg aatatataag
      301 aataagttgt aagatgtcag attttatgac attaagtttg tggttttcaa aatggtgatg
      361 tgagtgccca ctcacatgat aaggattaat aagagggagg gatattttta ataaatactc
      421 gtatctaaaa ttcttgacgc agcttttttg gtctcggatg tgattatttt gtagtccatt
      481 tttataagct ttctgttgta acatgaatag ctctgtttat catgcctatg attaaggctg
      541 cggcgagatt gctgtctatt tttctgaata ctttttcctt aattccatct gtgattgcag
      601 ctgcaaaaat atctttatat ttaaatttac cttgtttaat atatttaatt ccatagaaaa
      661 atagaacaag actaaatgtc ttcttgccac atttttaata aataatgatt atatttaatc
      721 taatacctat actgccccca tatgtagcgt aatagcggct atctctgtcg tgccagtcaa
      781 actacattcg acaccaattg caatcacgtt tgctaactaa aaattgatat ataatcagaa
      841 ctgttaattt aaaaatgtgc aggctggtta ttttggcatg tatattgctc ttctaaaact
      901 aaaccagata tgttaagcag tatctttatc ggaggtaatt tctatgaacg ttaacaacta
      961 tattttaata ttgtttttgg tattgttgat attgcctctc tcctgtaaag acgaaatacc
     1021 tgttaaaggc agttttccta atgatatatc acttgtagat cataccggaa aagagttctt
     1081 gttttctgac ctcaaaggta aagttctatt ggttagttac atatatacaa attgtccgga
     1141 tatatgccat ataataaata aaaaaatgaa tgtatttaaa tcacatatag caagagattc
     1201 aattagcaat gacgttgtgt ttgtatcaat ttctattgat cctcaaaatg atacacctga
     1261 aaaattaaga aaacatatcc atcatatgaa ttttgataca tcaaactggt attttgtaac
     1321 aggaaatatc ggaagcgttt acagattaat tgctaacgca gggatattcc ctgtaagaga
     1381 atctaaacaa aatgacgccg acaatgaaaa tgcatattta aattatataa tctctcatag
     1441 agacagaata agtttagtag ataaaaacgg gaatatcaga aaacattaca aaggaacaac
     1501 catggattct gatattattc tgatggatat gaaatcatta atttagttac caatattatt
     1561 aattcattgt attcacataa gatagttact ttaaagctat tttctaaaca ctaaaattac
     1621 tatatattat tagaagttac gtaattatgt ttaattcaag taatacgtgc tttaaaagat
     1681 taattctggg aggataatca ttaaagaacg aagagcatac cctagggtag aaatacgtga
     1741 agatgttatg tttggtgtga ataaacccgc acgttcaggt aaatccgaaa acctttcacc
     1801 gggtggcatg tgtattatat cagattcata cttacctcaa gggtctgaca tagtaattga
     1861 tataagtgtt aagcacacga ttacagatgg cagtgaaact tgggagaata ttactgttta
     1921 tggcaaggtt gcctgggtag acagtcctcc gggtaactat agcaaaatgg gtattgaatt
     1981 tagcagcaca aatgaaaaac tgcacatgat atatgagcat aagaaatctg agatttaaca
     2041 ggccatactt attttactca tttatgagtc cacttccatg gcgatgtatc taaaaggaaa
     2101 gaagcgtgtt agggttgaat tatcaatgaa atcttattcg taataaggga gataaatatg
     2161 tcagaattaa atgattcaat gagagaattt ttagcaggct gccattatgc cacactggct
     2221 acattaaatg atgacggttc ggttcatcaa actcctgtgt ggtatctatt taaagatgga
     2281 aacttttatt taacaacagg atcatcgggg agaaaggcta ggaatcttct caatcgatca
     2341 gaagccacca tattggtaga cagcagaaga aaccagggtc atgaacgatg ggtgagtgct
     2401 gttggaactg cagaaatcat taaaggcgaa aaatctaaag agataaactc aaagatcata
     2461 agccgttatc taactaaaga aggtatggac gatcccagag ttgctggcgt atttgaagca
     2521 tcaggggatg ctactatttg cattacgccg gattcatgga aggcatttga attcagcaca
     2581 gtcgacaagc aatttttcgg cggtatttta ggagaaactc ctgataagtg gtttaatgtt
     2641 gtggattaga tcgaatataa ataacataaa attgactgtc cttaattcta tgttattaaa
     2701 gcctatttag attcatctgc tacattgctt gccacgttca ctttgttcca tttcgttgcc
     2761 gccactgttc cctgccaagc ataatctatt gattcaccga gaattcttgc agcctcttga
     2821 ggagcatgaa atcccattga attttcggca gctatgaagt ccagccgcca ctgtgctttg
     2881 cgctgcatat caagcgcctt ttttaactgc acatctgttg cgccttcggc cctagctgtg
     2941 ttgatagcat cgagcagttc cataagcgat gctccggctc gttgcagcaa atcaaaatta
     3001 cgttgttgta ttccgtctac tcgttctttt atttcctgct cggaaaatct atgacaggtc
     3061 tggcaggctc ggttaatatt tagaagaggg cttctcaccc aatgatccga tactttggtg
     3121 gcgccatcac gcatataggg catgtgacaa tcggcacacc caactccact tcgcgcatga
     3181 attccctggc tccataactc aaactcgggg tgctgcgcct ttaatatctt tgctcccgtt
     3241 tccttatgtg tgtaatcaaa gaatttcctt ccgtcactta gagtcgtttc gtcccagaat
     3301 ttctcctgat cttccacctt aagtccgtta ccccagggga atgtaagttt catatctgcg
     3361 ccgcagtaat attccacatg gcactgcccg catacatatg agcgcatttc cgacctgctc
     3421 gcatctgtgt tgggattgta tggttcactc tttgaaccct ttctccactg ctcaatagaa
     3481 ggcaggtgtg gcgtgggagc atccgactca gctaatgcct gtataccctg aataaagccc
     3541 ggtcttgtaa cacgcagttc catggttttt ggtgaatggc aatcaacaca ggatacgggg
     3601 ttgccatgac ccatgtcatg taattgttta ttgagatctt tatacgaata tttatacgtc
     3661 tgctcgaatc cttttattac atcgccgttt ccaagctttt cgtagagcgg catgattgaa
     3721 gcatggcaat gcagacatga tccggactgg ggttttgttt ggcgttctgt ttgctcctga
     3781 tcttctagca tataagcatg tccgcggcgg tctctgtagt cgatggaaaa agcgtaaccc
     3841 ttgaacattc gctttagcca cgggtccctt tctatttttt gttcgggaag cgcttcgctt
     3901 cctccatggc cgccaaaacg cgtgcgtgta gccacgctgg tctttttata tgaatcgtac
     3961 tgcttgggcc agttgacttt ccacttttca gggtcagtat cagtttcagt aacttcaaca
     4021 agccggacaa aaggatttct agcttcgact ttccgctcaa aaatgttcag aagtaaagct
     4081 gtaatacccg cagtcaaaag cgctactgtc acaactataa cagcgattcc tgttttagat
     4141 ccaaattttg atgctccgct cattatttaa tacctcctgt ctcatgtgat tttatgggac
     4201 cgcccagtcc cgtagtttct ccgtgtccga ccgctttatg gcacttaaca catttgattg
     4261 catcgggggt tgcagtactg ccgtgggtaa gattttctgt tataccccta tgacaatcaa
     4321 tacagttgtt ctgcaatata atgctatttt tctcggttat cattataggt tcgtggaaat
     4381 tttgaagtgt aaacgctttg gaatgatgat atccgtttac agctttggat atgtatttgt
     4441 taacaaaatc atgaggcagg tggcactgga cgcaagtggc cgctgtgtgg tggcttgcct
     4501 tttgccatga atcatactga gactgcataa tgtggcagtt aacacaagac cttgggtctt
     4561 tactcagata tgaaaggcct tctccgtaat acatagtata actcccaaga ccgatcaaag
     4621 cccctacagc aatagatagg attattgctc ttttcttcat agtcataaca ggatctacac
     4681 aaacacgctt ttttaataac gctgataagg cttttaattc aatcaaccac gatacactct
     4741 ggatgatatg ccatagaaca tagcgagtca aatattggaa ataatgtgta tactatatta
     4801 ctaatctgat gctgcttttt tataagttca ggtcaaaagg tctaaggttt agggtctaac
     4861 aaaaaatata tgaccagatg taaaaaaata agattataat aataataatg acaaagaaac
     4921 tacctgaata tgatcatatg gttgaaataa aagaagacga aaaaaagatc tatgttttta
     4981 gaatctataa aaaatcaaag aaaaaacaac tttttacttg ttatgaatta ccggctaaat
     5041 cttttgatga agataaaaaa gctttccaag aatttgctaa aattctgggg gaaaacttat
     5101 taattgattc tcctactgca agaaagttgt ttggtatata aaagaaagag agggtaatcc
     5161 taccccttct ttttcttaac catatccggt tttgttctct ccggaattct ggcggctttt
     5221 ctggatagtc ctcttaggta gtagagcttt gctcttctta ctttaccaag acgcttaacc
     5281 tctatttttt caataagcgg ggaatgaagc gggatgatcc tttcgattcc tatgccgtat
     5341 gaaatttttc ttacggtaaa tgtttctcta aggccgccat ttttcttggc aattacaacg
     5401 ccttcaaaaa tctgaatacg ttctctgtca ccctctttaa tcttataatg tacggcaact
     5461 gtatcgccgg gtctgaactc ctcggcgttt tctctcaata atggcttctc aatttcgctt
     5521 acaataccca ctttacttac tcctttttat attttcacac tgcaaagtct gtcaaggatt
     5581 atgcttgcgg cactccttac tgacagatga ttaaatattt cacttccaac aacaggcttt
     5641 agattataat cagaaccctc aatgatttct cttatgagtc cataacctgt tccaaaaagc
     5701 aaaaggaacg gatttgaatc actttcgatt gtctctctca tttccaaata ccctgtcatc
     5761 ttatcactat accttgcgtc tgttactata atttttggct ttgagccttc taatttttca
     5821 atcgtttcta ttgtatcaga aaggctctcc gaaatatacg tatattccag cgattccttt
     5881 ctgctcgtgt tgtattcata tcccttaccc tttgtccagt gttccatcac agtgcggatc
     5941 agatccctct gctctgctac aggatgaaca aggtaaaacc ctttaacccc gtaagttctt
     6001 cccgctcttg ccatatcaag aacatcgatg ttggtaaagg atgtttttac aacatcaagc
     6061 cgtttattgt atacgggata atgcactaat gcaatataca aattactgtt tttttcaagt
     6121 tttctcttaa ggttatctac agtatttata tcgtctaatt ccagtatatt tcggtcgata
     6181 aggtctggtc tgctggcaaa agtcttctct atactctttt gtcttctcca gtcaccaatc
     6241 ttgccatgat cccctgatag cagtacctca ggcacttttg aaccctcaaa ttcctctggc
     6301 cttgtgtact gtggatactc aagcaggcct gagctgaatg attcatccac tgtggactga
     6361 tcattcccaa gcactcctgg gatgtatctt gctaccgaat ctatgatggc catggcggca
     6421 tactccccgc ccgaaagcac atagtcgcca atcgatagct ccatgtcaac gtatttttcc
     6481 tttatccttt catccacccc ttcgtacctg ccgcatatga atatgagggt ttcatagtcg
     6541 gctaaatcct ttgctatttc attgttaaat gtcttacccc cggggcttag caggattacc
     6601 ttgcttttat cgtccgagtt agttacgtct tttagagctg ctgcaatggg ctccggcttc
     6661 attaccatcc ctgagcctcc gccgtagggt ctgtcatcca ccatcctgtg tttatctgtg
     6721 gtgtagtctc ttatattata ggtgtttata ttcagcaagc ccgagtctgc agctctccca
     6781 ataacgcctg aggataacac gccttcaaac atctccggga atattgtaag tatattaaac
     6841 tgcatttttt ataagacgct ctaattagtt ttggtaatta ttagtattaa agatcaaaag
     6901 tccagagggg ggttatatta ataatgtatt ttttatagat caagaagccc gggcggggga
     6961 tcgaccacta tcttcttatt atcaatatcc acctctacga gtatcggctc tataaaagga
     7021 attaaaactt ctttgccgct agtaagctgg gcttcaagca gctcctgcgg ccctgagtcg
     7081 tttaatctta ttacctttcc aagaaggtta ttattagtat caaatacttc cagtccctca
     7141 agctcatagt aataaaactc atctcctcct gcaggctcta tttgagagtc atccacaaac
     7201 acttcggttc ccttgagttt atctgcttgc tcaatactgt ttactccctg cagtcttatc
     7261 acggcatgtt tacccgatag agaatgaccc tctatattat acttggtaaa ttcttcagta
     7321 tccccgtcct gtatgtatag agattctata aagtccaggt ttgatgtgtc accactatag
     7381 atgattagtc gcactcctcc ggctaatccg tgtgactttg aaataactcc gtaagggata
     7441 aaccccatga aaggttcttt gtcttaaggt tattctagga tttctaatac tgctcttttt
     7501 ttagcctttg cagcagcagc gcccaggata gttcttatgg cctttgcggt tttaccctgt
     7561 ttgcctatga tcttacccag atcttcctgt gctaccttta attccaaaac agttgtctgt
     7621 tcacctatta cttcctttac ctctacctga tcaggactat caacgagtga cttagcgata
     7681 taactaataa gctctgtgtg cttttccata acaataacct cctgtaaaac agcaatagta
     7741 tgtatttcta caattagtca gaaactaacc ctgtatacca gaaataattt tttgtaccct
     7801 gccggaatat tgcgcgccct tagacaccca atcatctatt ctgtctttct taatctctct
     7861 tttgttgggc tcggtt
//
LOCUS       DBNGSV010000060         3087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1881662, whole genome shotgun sequence.
ACCESSION   DBNGSV010000060 DBNGSV010000000
VERSION     DBNGSV010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1881662"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..992
                     /locus_tag="AC159U_02350"
     CDS             153..992
                     /locus_tag="AC159U_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3335927.1"
                     /translation="MKINLRKKAYVYLVLLLFILPGGADSHVGEHSLIKLFSDKIKTD
                     PENPVLYFKRGSQHRYAGHFEKALEDFSKARSLDEEYFLVDLGVGLVFLDYGWNKTAE
                     VYLKSFLKYSPDHIVGMTSLARAIARQKRGKESAALYEHISKILEKPAPAFYLEMADS
                     YLITNNYSAAIKTLDNGIKKLGHITSLDQKAYEIELNTKLTDKALLRLDNLIKYSPQK
                     ERWLYEKGQLLEQEDEYDKAKDAYLKALEHYSSRPIKRQNIPVLVRLKKNIDLALDRI
                     GQR"
     gene            1051..>3087
                     /locus_tag="AC159U_02355"
     CDS             1051..>3087
                     /locus_tag="AC159U_02355"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purple acid phosphatase family protein"
                     /protein_id="HIR3335928.1"
                     /translation="MNSSFIKKILFAAFSALLLLTIYLPFAKADTIYVPAGSTWKYLD
                     DGSDQGTAWTAPGFDDSGWASGPGILGYGDSQDTIVSFGPDSSNKYTTTYFRHDFNVA
                     NLNGIIALKLRLLRDDGAVVHLNGNEVHLSEMPETYDYQTFASSTAGGTEEDTYYESF
                     IDLNELLIGSNTLAVEIHQRTLTSSDISFDLELIGTDTIEVIRGPYLQQGSDSQAIVK
                     WRTNTPTNSVVLYGTDLGNLNQSKFDSAETSDHEIVIDNLSPDTKYFYAVGYNDTNQD
                     IIIAGDNTNHFFRTSPVPGTSKATKIWIIGDSGTANQNATNVKEAYLNSHVGDTDLWI
                     MLGDNAYNDGTDQEYQAAVFDMYPELLENTILWSTLGNHDGHSADSQTNTGPYYEIFS
                     LPTAAEAGGHASGTEAYYSFDYGNIHFVCLDSYDSDRSSNGAMLSWLENDLLSTNADW
                     VIAFWHHPPYTKGSHDSDTESALIAMRENAVSLLEDHGVDLVLSGHSHSYERSYLIDG
                     HYGNSDSFNPATMQLDGGDGSPNGNGAYEKQSSGLAPHEGAVYIVAGSSGKISSLDAS
                     GPHEAMFITLQQLGSVVLDINGNTLDAQFLTDTGAVNDSFTIVKGTVPTPTPTPVPPT
                     PTPTPVPPTPTPTPIPTATPTPVPPTPTPTPEPTATPTPVPPTPTPTPTPLPTAT"
BASE COUNT          983 a          628 c          615 g          861 t
ORIGIN      
        1 gcaagccctt ttgctgtatc tttttctaca gggagatatt gatacaaagc gattgatata
       61 gaacttaata caattagaat tagtaaattt ttcacaattt ttatttctgg tatttataaa
      121 agtagtaaaa tactatatta atatatctat atatgaaaat taatttacgt aaaaaagcat
      181 atgtatatct agtattgtta ttgtttatat tgcctggtgg ggctgactct catgtgggtg
      241 agcatagtct tattaagtta ttcagcgata aaataaaaac cgatcctgaa aatccagttc
      301 tctattttaa aagaggctcc cagcacagat acgcagggca ctttgaaaaa gcccttgagg
      361 atttcagtaa agcccggtca cttgatgagg agtatttcct ggtagatctt ggagtagggc
      421 ttgtgttctt agactacggc tggaacaaaa cagccgaggt ttaccttaag agttttctta
      481 aatactctcc tgatcatatt gttggaatga catctcttgc aagagcaata gctcggcaaa
      541 aaagaggcaa agaatctgcg gctctatatg aacatatatc taaaatttta gaaaaaccag
      601 ccccggcatt ttatctggag atggcagatt cttatttaat taccaataat tattctgcag
      661 ccataaaaac tcttgataac ggtataaaaa aacttggtca tattacatcg cttgaccaaa
      721 aagcatatga aattgagctt aatactaaat tgacagataa agccttattg cgtctcgata
      781 atctcataaa atattcgcct caaaaagaaa gatggttgta tgaaaaaggg cagcttctgg
      841 aacaggaaga tgaatatgat aaggccaaag atgcctatct aaaggcacta gagcattaca
      901 gttccagacc aataaagcgg cagaatattc ctgtgcttgt tagacttaaa aaaaatattg
      961 atttggcact tgatagaata gggcaaaggt agtacaatta gcctaatacc agagcattaa
     1021 tcaaattaac aataattcgg ggagaaatat atgaattctt catttattaa aaagatctta
     1081 tttgccgctt tttcagcact attattactt accatttact tgccatttgc caaagcagac
     1141 acaatatatg tgccggcggg ttctacctgg aagtatcttg atgatggctc ggatcagggc
     1201 acagcatgga ccgccccggg atttgatgat tccggatggg cgtcaggacc cgggatattg
     1261 ggatacggtg atagtcagga tactattgtg agttttgggc ccgattcaag caataaatat
     1321 acaactacat actttcgtca tgattttaac gtagcaaatc taaacggcat aattgcacta
     1381 aagctgaggc tacttagaga cgacggcgct gttgtacatc taaatggtaa tgaggttcac
     1441 ctaagtgaaa tgcctgaaac ttatgattat cagacttttg cttcatcaac ggcaggcggc
     1501 actgaagagg atacttatta tgaatcgttt attgatctga atgaactttt gatcggcagc
     1561 aatactcttg cagttgagat acatcagaga acacttacga gttcagatat aagctttgac
     1621 ttagagctta tcggtactga taccatcgaa gtgataaggg ggccatatct gcagcagggt
     1681 agcgattcgc aggctatagt taaatggaga acaaacacac caacaaacag cgtagttctt
     1741 tacggtacgg atttaggcaa tctcaatcag tccaagtttg attctgcaga gaccagtgac
     1801 catgagattg ttattgataa cctgagtcct gatactaaat atttttatgc agtaggatat
     1861 aacgacacaa atcaggatat aataattgca ggtgataata caaatcattt cttcaggact
     1921 tcacctgttc caggcacttc caaggcaacc aaaatctgga tcatcggcga ttcgggtaca
     1981 gcaaatcaaa atgctacaaa tgtaaaggaa gcttatttaa attcacatgt aggagataca
     2041 gatctgtgga taatgctcgg agacaacgca tataatgatg gtactgatca ggagtatcag
     2101 gcagccgtat ttgatatgta tcctgagctt ttagaaaata cgatactctg gtcaactctt
     2161 ggaaaccatg acggccacag cgcagattct caaactaata caggacctta ttacgaaata
     2221 ttttcacttc ctactgcggc agaagcaggc gggcatgctt ctggaacaga ggcttactac
     2281 tcttttgact atggtaacat acattttgtc tgtctggatt cctatgacag tgatcgctca
     2341 tcaaatggag caatgctctc ttggcttgaa aatgatctgc tttccacaaa tgcagactgg
     2401 gttattgctt tctggcacca tccgccttat accaaaggtt cacatgattc tgatactgag
     2461 agtgctttga tagcaatgag agaaaatgca gtttcccttc ttgaagatca cggggtagac
     2521 ctcgtacttt ctggtcacag ccattcttat gaaagaagct atttgattga tgggcactat
     2581 ggaaattccg attccttcaa tcctgcaaca atgcagcttg atggaggaga cggaagtccg
     2641 aacggaaacg gagcatacga aaaacaatca tccggcctgg cccctcatga gggtgctgta
     2701 tacatagtag ccggaagctc agggaaaatt agcagccttg atgcaagcgg tccgcatgaa
     2761 gctatgttta taacacttca gcagctgggt tcagtagtac tggatattaa tggaaataca
     2821 cttgatgcac aatttttaac cgatacaggt gcagtaaatg atagctttac aatagtaaag
     2881 ggcactgtac caacacctac accaacacct gttccaccca ctcctactcc tacacctgtt
     2941 ccaccgacac ctacacctac acctatacct acagctacac cgactcctgt accaccaact
     3001 ccaactccta cacctgaacc aacggctaca ccaacaccgg ttccaccaac tcctacgcca
     3061 acaccaactc ctttacccac agcaaca
//
LOCUS       DBNGSV010000061         8988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_288638, whole genome shotgun sequence.
ACCESSION   DBNGSV010000061 DBNGSV010000000
VERSION     DBNGSV010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_288638"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="AC159U_02360"
     CDS             <1..1320
                     /locus_tag="AC159U_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3335929.1"
                     /translation="ISGKFCYYDDNIIGLYPDDEELKKYDAQSYLGIPIYNSKNSVIG
                     HLAVFHDDIFKDISNFFHILQVFALRAGSELERIEAEDSLRRSHDQLEGAFEEIKSLK
                     QKLEKENVYLKEEIKTEYNFEEIIGTSKPLKEVQNNIKQVADTDATVLIRGETGTGKE
                     LISRAVHNLSSRNNGSLIKVNCPAIPSGLIESELFGHEKGSFTGAITKKIGKFELADG
                     GTIFLDELGDLPLDAQAKLLRVIQEREFERVGGTETFTVDVRVIAATNRNLEDAVKEG
                     KFRADLYYRLNVFPIDIPSLRDRTEDITPLCYYFLDKYCRKMGKNINSISNDTLKRLT
                     DYNWPGNIRELENIIERAVILSTSDTLEIDNRLLVSSQKLQADEGIGSTRIEDVEREH
                     IIKVLSQTNWQVHGKNGAAKILDINPSTLRTRMAKLGIKKKAIQQDQ"
     gene            1527..2093
                     /locus_tag="AC159U_02365"
     CDS             1527..2093
                     /locus_tag="AC159U_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HIR3335930.1"
                     /translation="MSNIRYLNSAAAGIFVIAVALMVFAAANLSAKDKTPADLNDLEI
                     AHVAYTADSIDIRYAHLALAKSKNPVIHEFAKTMIRDHEAVNAQALALLEKLGAEAQD
                     NFLSQQLNTQADGLVEKMSGLSGNDFDKFYAENELGYHQAVNGLVGGTFIPNIQNAEV
                     KALFEQALQIFKAHEKHAEKMVASLNGK"
     gene            2097..2471
                     /locus_tag="AC159U_02370"
     CDS             2097..2471
                     /locus_tag="AC159U_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3335931.1"
                     /translation="MKKSNLTVWAAAAFVAAVLSLTTTWTALADEPQSKVHVVEIRNL
                     EFIPAELQVLPGDTVEWVNKDFIPHTATANDESWDSGLLNKDDSWKLTVTSDTQTNYF
                     CRFHPNMKGQFVLTIKKEHKKR"
     gene            2483..3139
                     /locus_tag="AC159U_02375"
     CDS             2483..3139
                     /locus_tag="AC159U_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3335932.1"
                     /translation="MFELGGLSIDRPDTKYNVTYIDMSDEKLAGLYVKNKDELAFNEL
                     VSRFGDKIYRLSLRITKSPQSAEEVLQLVFIKLIEKLGDFRGSSKLATWIYTITKNES
                     FNLLRKMNNYSRTHLSSDNNYSNEDLPNYEFAGIDTDNNPENIAINSEQTEFLDKAIN
                     SLEPVYRLAYQLRDIEGLTNKEAAQVLGISISAVKSRILRARNELKEKLTRSYPEYRS
                     "
     gene            3331..3648
                     /locus_tag="AC159U_02380"
     CDS             3331..3648
                     /locus_tag="AC159U_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3335933.1"
                     /translation="MPNKRTSKRQILRTKVRFGRTEPSSLGNTFNVSPRGIGITTSRL
                     FPKNAKLKLDIYLDDPQDSFDQKVVKTEGRVAWIKHGFPGQPSKMGIKLDNPKFDLLL
                     SKK"
     gene            complement(3659..4243)
                     /locus_tag="AC159U_02385"
     CDS             complement(3659..4243)
                     /locus_tag="AC159U_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335934.1"
                     /translation="MIANKLMLATLLFFVCTCMTYAQDDIFKYDMSLAMDRGDAKKAL
                     ENAGFEIENDTRVSKKLRSFFVAGSIYSSISDPHDTDKSTELDFYDNRLMNTTLTVKS
                     KDYIANTGYTNTFLSELTKIYGKHSAYEKVMSIKSWMWMAGNTKILLNSDSRKKTAKI
                     SFIYLPLYSKKYEDNVKVKLKGKPFDPVDDMILK"
     gene            4366..5304
                     /locus_tag="AC159U_02390"
     CDS             4366..5304
                     /locus_tag="AC159U_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3335935.1"
                     /translation="MGYRRKSYRKKSSKPIVLFVLISILLHILILFLLSNKSDFLLPK
                     INKAEKKDDSFIEISEIPIPKEKETEPPEKTTRYADRSRKVEKEKTRDEFTKRGSIAP
                     PIAQKPAAQVSKPKEQVKKEQIAKVEKKAEKSKQKTVKTASIPKYKRSDFNLPEKDKK
                     EQKDTNNQNNTDKLFQTAQNPLFNPPASSPDFKGLRNLPKEDTVDWNTTEYKYLSYFL
                     KIKRQIEGVWSYPKASKLKGEQGELLLIFTLSSNGQLEDVKLLETSGFPRLDNEALRA
                     IRVAAPYVPFPKAWELERLNIKAGFIYRFGNWQLRR"
     gene            complement(5310..5915)
                     /locus_tag="AC159U_02395"
     CDS             complement(5310..5915)
                     /locus_tag="AC159U_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3335936.1"
                     /translation="MSIEDKLAQIILARHCKTTWNLEKRLVGISDQPLCEFGLNEAHE
                     TAPKLKSLGLDRIVSSPLRRAHHTSEIYSKFLGIPLTINEGLREIDHGEWNGIKLAEL
                     LEDETSDFKIWYNDPTKIPIPGGTEPILDAQERIKKTIKEILLAYPAETVLVVMHKHI
                     RSIFTCFLNGLHLSNFRENIDDSVLPIELSKEQLISAYGLE"
     gene            complement(5952..7586)
                     /locus_tag="AC159U_02400"
     CDS             complement(5952..7586)
                     /locus_tag="AC159U_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TubC N-terminal docking domain-related protein"
                     /protein_id="HIR3335937.1"
                     /translation="MSTLADLDLKTIMKLTGIPTQKDLVNPKEPLQMAKKVRVTFRPL
                     PDGYSNKEIIKFREVLQQKLKECGVETLTWEESTEKPTGSFINRFILGRRVKSNVHAV
                     IDVKREYSIIRKAFSNFAEFIYGTMRDPERSVMGILKISGWADNFTARWLADPYNTQV
                     VTLKSLDSEFIDKETPYDRKIVIGLQDLISTMSEIVIGISHDKFSIVNMNLSDSSYTH
                     EEIDDFIKKSFIPKIYAPIKPPVLNRFIQSEFEPSESKFANRLSELGKQLKKTDLFPH
                     GSKFSDKIPRQSHRDVVEKILEGRTGVSYGFIALVESPQYEGKKIITPQKWAKLLEIK
                     NINNEYVREDSDGRWYIKSVIRGKTIYQQVPDIWICTSRSGSDKTNLDTKSDVVRVGL
                     IKGKLYLQTPKGVDLKRRDIRPSFDTYVILAQALSCALYTPEIIEDGIPIVHFHGYPD
                     PQWFSKNEYHIGAQNPSMPCGTIEAALLNFAGVYDIANENGEIMNLLCLVESDHGVNI
                     LGPKPEYIVERLMEGSASGDIMLGGRFLPELKKAGV"
     gene            complement(7698..8429)
                     /locus_tag="AC159U_02405"
     CDS             complement(7698..8429)
                     /locus_tag="AC159U_02405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3335938.1"
                     /translation="MFNTIKGHVSILSVTVIFGTLAMILSLINKTWTFKYAVKPWGTY
                     ILKGCGVRLDIEGLEHLPQDPCILMYNHSSSFDIFAFAAGVPFEWRAIMKDEVAKIPF
                     VGWVCRLTGQYFVARDGGSRDLNMVKEIVEKIKHGPSVLVAPEGTRSKDGNLLPFKKG
                     GFVIALQAKVPVVTMVITGGNDVYPKGSKQIKPGVMNIKYFEPIHVDELGRGRQAREQ
                     LEKVVRGQMISVLGEDKDTQVTDEG"
     gene            complement(8464..>8988)
                     /locus_tag="AC159U_02410"
     CDS             complement(8464..>8988)
                     /locus_tag="AC159U_02410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3335939.1"
                     /translation="VDVGQLGNEFFANGIGFGMDGAVAHRFKDYSMIRGELAYILGAV
                     IEAFSFPGFRADVKIGQWRMSGNLLLSGASNGSYHGGKFQLAPDAKVDDGLLDMYIIK
                     NMPTLSRLIKIPKVLSGTHTSLEEVFIKASTSMEVTLDEPIPAHMDGEPFILQAGYHK
                     IEVREKALKVLSAK"
BASE COUNT         2856 a         1853 c         1697 g         2582 t
ORIGIN      
        1 atatctggta aattttgcta ttacgatgac aatattattg gtttatatcc tgatgatgaa
       61 gaattaaaga aatatgacgc tcaaagctat ctaggtattc ccatttataa ctcaaagaat
      121 tcagttattg gtcatttagc agtatttcac gatgatatat ttaaagatat ttccaacttt
      181 tttcacattc tacaggtatt tgctctaaga gcaggatcgg agctagaaag aattgaagct
      241 gaagattcac ttagaagaag tcatgaccag cttgaaggtg cttttgagga aataaaatca
      301 cttaagcaaa aacttgaaaa ggaaaatgta tacctaaaag aggaaataaa aacagagtac
      361 aattttgaag agattatagg aaccagtaaa ccgttaaaag aagttcaaaa caacatcaaa
      421 caagtagcag acacagacgc tacggtactt ataagaggtg aaacaggaac aggcaaagag
      481 cttatttcca gagctgtaca caacctgagc agcagaaaca acggatcgct cattaaagtt
      541 aactgtccgg ctattccatc gggcttgatt gagagtgagc tattcggaca cgaaaaggga
      601 tcattcaccg gtgctattac taaaaaaata gggaaatttg agcttgcaga cggaggtacg
      661 atattcttag atgagcttgg cgacctgccc ctggatgctc aggcaaagct cctgagagtc
      721 atacaggaaa gagagtttga aagagtcggt ggcacagaga cctttacggt agacgtaagg
      781 gtaatagcgg ctacaaacag gaacctggaa gatgcagtta aagaaggcaa attcagagca
      841 gatctttatt acagattaaa tgtgttccct atagacatac catctttaag agacagaact
      901 gaagatataa cacccttatg ctattatttc ttggataaat attgcagaaa aatgggaaaa
      961 aatattaatt ctatcagtaa tgatacactt aaaagattaa ccgattacaa ctggcctgga
     1021 aatatcaggg agcttgaaaa tatcatagaa agagccgtaa ttctcagcac aagcgatact
     1081 cttgaaattg ataacagact attggtatca tcgcagaaat tacaagcaga tgagggtatt
     1141 gggtcaacaa ggattgaaga cgtagaaaga gagcacatca tcaaagtact aagtcaaaca
     1201 aactggcagg tacatggaaa gaacggagct gccaagattt tggacatcaa ccccagcact
     1261 ctcaggacac gcatggccaa gctcggaata aagaaaaaag ccatacaaca ggatcagtaa
     1321 atcaccctcc atacaaaata attactctat accattacat tgcgttattt cgcaattaac
     1381 agattttaac atgatcttat ctaatatcat ttaaatcata attatttcaa tcatttcaga
     1441 cagttacaag cttattttca gttgtcttaa gtttggcaca ggtgttgcca tatatgtata
     1501 taaaatttat tggaggtaac accataatga gcaacataag atatttaaat tcggcagcag
     1561 ccggtatttt tgttatagca gtagcactca tggtatttgc agcagcaaac ctgtcagcca
     1621 aagacaagac cccggctgat ttaaatgacc ttgagatagc gcatgtagca tacacagcag
     1681 attcaattga cataagatat gcacacttag cactcgcaaa gtctaagaat cctgtaattc
     1741 atgagtttgc aaaaaccatg ataagagacc atgaagccgt aaacgctcag gcgcttgctc
     1801 ttcttgaaaa actaggtgct gaggcacaag acaactttct aagccagcag ctaaatactc
     1861 aggcagacgg gcttgtagaa aaaatgagcg gattatcagg aaacgacttt gataaatttt
     1921 acgctgaaaa tgagcttgga tatcatcagg ccgtaaatgg tcttgtcgga ggaactttta
     1981 tacctaatat ccaaaatgcg gaagtgaagg ctcttttcga acaggctctg caaatattta
     2041 aggctcatga aaagcatgca gaaaagatgg ttgcttctct aaacgggaaa taagtcatga
     2101 aaaaatcaaa tctaacagtt tgggctgcag cagcttttgt tgctgcggtc ctgagtttaa
     2161 cgacaacctg gacagcctta gctgacgagc ctcaaagcaa ggtgcatgta gtcgagataa
     2221 gaaatcttga gtttatacca gctgagctgc aagtattgcc cggcgatacc gtagagtggg
     2281 taaataaaga ttttatccct cacactgcca ctgcaaatga tgaaagctgg gactccggtc
     2341 ttcttaataa ggatgacagc tggaaattaa cagtgacctc agacactcaa acgaattact
     2401 tttgcaggtt tcatccaaac atgaaaggtc agtttgtatt aacaatcaaa aaagagcata
     2461 aaaaaagata aggagcacag acatgtttga acttgggggc ttatcaatag accgtcctga
     2521 tactaagtac aatgtaacat acatagacat gagtgatgag aaacttgcag gcctttacgt
     2581 aaaaaataaa gatgaacttg catttaacga attggtaagc aggtttggag ataaaattta
     2641 caggctgtcc ctaagaatca ccaaatcacc tcaatcagca gaagaagtct tacagttagt
     2701 atttataaag cttattgaaa aattgggtga tttcaggggc agctctaaat tagctacctg
     2761 gatatacaca attaccaaaa atgagagctt caatctttta agaaaaatga ataattactc
     2821 ccgcactcat ctaagttcag ataataatta ttccaatgaa gacctgccca attatgagtt
     2881 tgcgggaata gacacagata ataacccaga aaatatagca ataaactctg agcaaactga
     2941 atttttggat aaagccatta acagcctgga gccagtttac agattggcat atcaattaag
     3001 agatatagaa ggacttacta ataaagaagc agcacaagtt ctgggtattt ctatttctgc
     3061 agttaaatca cgaatcctaa gagcaagaaa cgagcttaaa gaaaagctca ccaggtcgta
     3121 ccctgaatac agatcataat atgtataatg ttttaataat tttatccata ttttcaatac
     3181 gcaaatatag ttaatactat tgttatatag tataaaaaaa taaaactata ctattgcccc
     3241 attgacatta tttaaaaaat ttgttcaaat tactatatgt tgagagaatt gcaataaatt
     3301 acactaaagt gtatcaaaga ggttattgcc atgcctaaca aaagaacatc aaaaaggcag
     3361 attttgagaa ccaaagtgcg gtttggaaga actgagcctt cgagccttgg caacacattc
     3421 aatgtgtctc caagaggtat aggtataaca acaagcagat tatttcccaa aaatgctaaa
     3481 ctaaagctgg atatatatct ggacgatcca caggatagtt ttgatcaaaa ggttgtcaaa
     3541 accgagggca gagtagcctg gatcaaacac ggatttccag ggcagccgtc gaagatgggt
     3601 ataaaattag ataatcccaa atttgatctt cttcttagca agaaataaat tgctgcagtt
     3661 actttagtat catgtcatct accggatcaa atggcttacc cttaagcttt accttcacat
     3721 tgtcttcata ctttttagaa taaagcggca agtatataaa acttatcttt gcagtcttct
     3781 ttctgctgtc agaatttaaa agtattttag tattgcctgc catccacatc catgatttaa
     3841 tgctcatcac tttttcatat gcgctgtgct tgccataaat ctttgtaagc tcggatagaa
     3901 aagtattggt atagccggta tttgcaatat aatccttact ttttactgta agggtggtat
     3961 tcataagcct attatcataa aaatcaagct cagtgctctt atccgtatca tgtggatcac
     4021 taatgctgct atatatagag ccagctacaa aaaaactcct caatttcttg gatactctgg
     4081 tatcattctc tatctcaaac ccagcgtttt ccaaagcctt ttttgcatcc cccctatcca
     4141 ttgcaaggct catatcatat ttgaatatat catcctgcgc ataagtcata caagtacaca
     4201 caaagaaaag taatgttgcg agcataagtt tgttagctat catattaata tctccaaaat
     4261 gttaagacgt aatgttataa tagaaaagag cacaattata actgctaagc gctaaatttg
     4321 tactattaat aatcttctct gaatatatac tatctaataa tataaatggg atacagaaga
     4381 aaaagttaca ggaaaaaatc atcaaagccg attgttctgt ttgtattaat atctatattg
     4441 cttcatattt tgatactttt tcttttatca aacaaatcgg actttctgct tccaaagata
     4501 aataaggctg agaaaaagga cgacagcttt atagaaatct cagagattcc tatacccaag
     4561 gaaaaagaga ccgagcctcc tgaaaaaact acccggtatg ctgacagatc acgaaaagta
     4621 gaaaaggaaa agacaagaga cgaatttacc aaacgcggct ccatagcccc gccaatagca
     4681 caaaaacctg ccgcacaggt ttccaaacct aaagagcagg ttaagaaaga acagattgca
     4741 aaggtagaaa agaaagcaga aaaatcaaaa caaaaaacag ttaagactgc ttctattcca
     4801 aaatataaac gctccgattt taaccttcca gaaaaagata aaaaggagca aaaggacacc
     4861 aacaatcaaa acaacacgga caagcttttt caaacagcac aaaacccgct gttcaaccct
     4921 cctgcaagtt caccggattt caagggactg agaaacctgc caaaagaaga cacagtggac
     4981 tggaacacta ccgaatataa atatctgtcc tattttctaa aaataaaaag acagatagaa
     5041 ggtgtgtgga gctaccccaa ggcatcaaaa ctaaaaggcg agcagggaga gcttcttctt
     5101 atttttactc tcagcagtaa tggacagtta gaagacgtaa aacttctgga aacctcgggc
     5161 tttccgagac tggacaatga agctctaagg gctataaggg tagcagctcc atatgttccg
     5221 tttccaaagg catgggagct tgaaagacta aatatcaagg ccgggtttat ttacaggttt
     5281 ggaaactggc agcttagaag ataagagatt tactctagtc cgtaagcgct tataagctgc
     5341 tcttttgata gctcaattgg aagcacgctg tcatctatgt tttctctaaa gttactaaga
     5401 tgaagcccgt ttaaaaaaca ggtgaatata gaccttatgt gtttgtgcat cactactagc
     5461 actgtctctg caggataggc tagaagtatt tcctttattg ttttctttat cctctcctga
     5521 gcgtccagta taggctcagt acctccggga ataggaattt ttgtgggatc gttataccag
     5581 atcttaaagt cagatgtttc atcctccaaa agctcggcta acttaattcc gttccactcc
     5641 ccgtggtcta tctctcttaa accctcattt atggtaagcg gtataccaag gaatttcgaa
     5701 tagatctccg aggtatgatg cgcccttctg agggggcttg agactatcct gtcaagacca
     5761 aggctcttga gtttcggcgc tgtttcatgc gcttcattca ggccaaactc acatagaggc
     5821 tgatcggata ttccaacaag ccttttttcc aggttccatg tggtcttgca gtgccttgca
     5881 agtattatct gtgctaattt atcttctata ctcatatttt ccggtctctg tttattaatt
     5941 gattaattta attatacgcc tgcttttttt aactccggaa ggaatcttcc gccaagcata
     6001 atatcacccg atgccgagcc ttccattagc ctttctacaa tatactcagg cttaggccct
     6061 aagatgttta ccccgtgatc agattccacc aaacacagaa ggttcatgat ttctccattt
     6121 tcatttgcta tatcataaac cccggcaaag tttagaagcg ctgcctctat cgtaccgcat
     6181 ggcattgacg ggttttgagc gcctatatga tactcattct tgctaaacca ctgcgggtcg
     6241 ggataaccat gaaaatgtac tatgggtatt ccgtcttcaa ttatctcagg ggtataaagg
     6301 gcacatgaaa gagcctgtgc cagtattacg taagtgtcaa aagacggccg tatatcacgt
     6361 cttttcagat caaccccttt gggggtctgc aaatagagct taccctttat aagccctacc
     6421 ctcaccacat cagacttcgt atcgaggtta gtcttatcgc ttcctgatct cgaagtgcag
     6481 atccagatat cgggaacctg ctgatatatt gtcttgcctc taattaccga ttttatatac
     6541 cacctgccat ccgaatcttc tcttacatat tcattgttta tatttttaat ttctaagagt
     6601 tttgcccatt tctgaggtgt gattattttt ttaccttcat actgcggaga ttcaaccaga
     6661 gcaatgaagc cataagatac gccggttctt ccttccagta ttttttcaac aacgtccctg
     6721 tgggactgcc tggggatctt gtcgctgaac tttgagccat gaggaaagag gtctgtcttt
     6781 ttaagctgct ttccaagctc ggataacctg ttagcaaatt ttgattctga aggctcaaac
     6841 tcgctttgaa taaacctgtt gagcacagga ggctttatag gagcatatat cttgggtata
     6901 aaagattttt tgataaaatc atctatctct tcatgagtat aagacgaatc ggacaggttc
     6961 atatttacaa tcgaaaactt gtcgtgcgat atacctatca cgatttcaga cattgtggag
     7021 ataaggtcct gcaggccgat tactatcttt ctgtcataag gggtctcttt atctataaac
     7081 tccgaatcca gcgatttcag ggtaacgacc tgagtgttgt atggatcggc tagccacctt
     7141 gcagtgaagt tatcggccca gccgcttatc ttgagtattc ccatgaccga tctttcagga
     7201 tcgcgcatcg ttccgtagat aaattcagca aaattgctaa acgcttttct gatgatggaa
     7261 tattcgcgtt ttacatcaat aacggcatgt acgttgcttt tgactcttct gcctaatata
     7321 aaccggttta tgaatgatcc ggtgggtttt tctgttgact cttcccaggt tagggtttct
     7381 acgccgcatt cttttagttt ttgctgcagt acttctctaa actttataat ctctttattg
     7441 ctatatccgt ctggaagagg cctgaatgta accctcactt tctttgccat ctgaagaggc
     7501 tcttttgggt ttacaagatc cttctgggtg gggatccccg taagtttcat tatagtcttt
     7561 aaatcaaggt cagctaatgt agacataaac tcctagtact ctgattaaat ttgaaaacga
     7621 aaaccctgta ttgtaatgaa taataattat ttctcaatat ataaataaag gtttataaaa
     7681 atgacaggat tgtggtctta accctcatcg gtaacctgcg tgtccttatc ctcgccaaga
     7741 acagatatca tctgccctct taccactttt tcaagctgct cccttgcctg ccttcccctt
     7801 ccaagctcgt caacatgtat gggttcgaaa tactttatat tcataacccc gggcttgatc
     7861 tgttttgaac ctttgggata tacatcattt ccgccggtaa tcaccatggt caccacagga
     7921 accttggcct gtaaagctat tacaaacccg ccttttttaa atggcagcag attaccgtct
     7981 ttacttcttg taccctcggg ggcaaccagt acagaggggc cgtgctttat tttttctaca
     8041 atctctttta ccatgtttaa atctctcgag ccgccgtctc ttgccacaaa atactgtccg
     8101 gtaagcctgc acacccagcc cacaaaagga atcttggcta cctcatcctt cattattgcc
     8161 cgccattcga agggcacccc tgctgcaaag gcaaaaatat caaacgagct tgaatggtta
     8221 tacataagaa tgcacgggtc ctgcggaagg tgttcaagcc cttcaatgtc aagcctcacc
     8281 ccacagccct ttagtatata tgtaccccag ggtttcaccg catatttaaa tgtccatgtc
     8341 ttattaataa gtgaaaggat catggccagg gtgccaaaga tcaccgtcac cgacaaaata
     8401 gatacgtgtc cttttattgt attaaacatt tctaaaaata cctcttatta tctgtaatag
     8461 ttcctatttt gcagaaagca ccttaagcgc cttttccctt acctctattt tatggtatcc
     8521 ggcctgtaaa ataaagggtt cgccgtccat atgtgccggt attggttcat caagagtaac
     8581 ttccatagat gtgcttgctt ttatgaaaac ctcttcaagg cttgtgtgcg tgcctgagag
     8641 tacctttgga atcttgatga gccttgagag tgtcggcata tttttaatta tatacatatc
     8701 aagcagcccg tcgtccactt ttgcgtctgg agcaagctga aacttgcctc catgatatga
     8761 gccgttcgat gcgcctgaca gaagcaaatt accgctcatt ctccactgcc ctattttaac
     8821 atcagccctg aatcccggaa atgagaatgc ttctataaca gcaccaagaa tgtatgcaag
     8881 ctctcctctt atcatgctgt agtccttaaa cctgtgtgct actgctccgt ccatcccaaa
     8941 ccctatgccg tttgcaaaaa actcgtttcc caactgcccc acatccac
//
LOCUS       DBNGSV010000062         4229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_81350, whole genome shotgun sequence.
ACCESSION   DBNGSV010000062 DBNGSV010000000
VERSION     DBNGSV010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_81350"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..510)
                     /locus_tag="AC159U_02415"
     CDS             complement(97..510)
                     /locus_tag="AC159U_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335940.1"
                     /translation="MKLSNSQLEQIRRITKVIRDPAIQQALRVQYENRNLIKYAINLA
                     QHHQESIKHILNTTGTSSIFKDILEAIPQGSIHEQTYRAIEAVELMKSQIDDVTLNQL
                     NNLHDYIDTNNLLQNSLLLDSHTLKSDPHPKAVPG"
     gene            complement(570..1433)
                     /locus_tag="AC159U_02420"
     CDS             complement(570..1433)
                     /locus_tag="AC159U_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335941.1"
                     /translation="MLSKKIIVLFDEYGNHTFNLKSNRIFVGVSAIYELEDEEDIFKK
                     SDKIMGLSKSKPLKNDKINVSKAITISNELIKYPIEIDVRFINLDDSILINNLDNYKA
                     FGDYTRKEYRGITKERKYAQILYPQLVETCLFSSISHFLEHNTEDIYDIEICVDNWEY
                     PIPDIDLALKYASEKLELSISDLMNKFKINSIVTISNKQLLKDISSERKRFIDVITSV
                     ISKNFRDHNDKTYSSKPLEILTKGMGNKIRVEDSTEDSTAETSNSFYDSIVNNVEETR
                     NKDNKIMLINN"
     gene            complement(2513..3064)
                     /locus_tag="AC159U_02425"
     CDS             complement(2513..3064)
                     /locus_tag="AC159U_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR3335942.1"
                     /translation="MYISKSTIRGFTLIEVVTIVGIISLLIVIAGSFSSQFFQRRDID
                     QLTNAVSGEVQISKLKAARDGVEYRLSMTISDDEDTIEIKRERGNSNSLSSNWTEISS
                     QMLKLEKYIDITIVPSNPFVIKPDGSLTLDPSSATESSCVISPKDGTKFKRCGQVSVT
                     RLGHVNIIKGNWDGSDCKQIKDK"
     gene            complement(3104..>4229)
                     /locus_tag="AC159U_02430"
     CDS             complement(3104..>4229)
                     /locus_tag="AC159U_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335943.1"
                     /translation="ATGEIIFKFHPSDSTLTNSNLMVCEVASEVGVFDLNADGYMDVA
                     YVGDTCGRMWRFDVSMPIETDSEATSSDLALKSLDTGISAPDWTGSVAFCAGSDANCL
                     VDEMDEFSDPIVVTTDASAVPSLFPIYFPPSVVIDDIGRKHVIFQTGDRREPTDNEKF
                     GKLYNFIDDFIPAFLAGGTAVQASQVKTEEYLTSRDLVVNLSAQSGVANQFEAVSVDQ
                     TAINANGEFLVNFPGGSNTLGEKGIGRPIVANRILLFSTFEPDALLDNSCSAGPGVGR
                     IFALDYLTGESALARIPGSDKIISGSDTQKAAAAGKSVGVGMPTPASLTFGERGSLVM
                     TIAFTGGVSGDGGDSGASFIVITIADFPQRTQTLYWEELI"
BASE COUNT         1357 a          779 c          640 g         1453 t
ORIGIN      
        1 gagagattac aacgagtaca gacctcattc ggcacttgat aatatgacgc cggaggagta
       61 tgttaaacaa cacataaaaa agccggaaat ctctaattat cccggtacag cttttgggtg
      121 agggtcactt tttaatgtat gactatcgag aagaagagag ttttgaagca aattatttgt
      181 atctatataa tcatgcaaat tgttaagttg gtttaatgtt acatcatcaa tttgactttt
      241 catcaattca accgcttcga tagctctata tgtttgctca tgtatagagc cttgaggtat
      301 tgcttcaagt atatctttga aaatacttga tgttcccgta gtgtttagaa tatgtttaat
      361 agattcctga tgatgttgtg caagattaat agcatatttg attaaatttc tgttctcata
      421 ttgcactcgt agggcttgtt gaatagctgg gtcacgaatt actttagtta tacgtcttat
      481 ctgttctaat tggctattac ttaatttcat tacattgtag taattaattt tcaaaaattt
      541 gatatgacca gaaataataa taaaataatc taattattaa ttaacattat tttattatct
      601 ttatttctag tttcttctac attatttaca atagaatcat aaaaactatt acttgtttct
      661 gctgtactat cttctgtact atcttcgacc cttattttat tacccatacc tttggtcaat
      721 atctcaagag gctttgaact gtatgtttta tcgttatggt ctcgaaaatt tttgcttatc
      781 acactggtaa taacatctat aaatcttttt ctttctgaag atatatcttt taataattgt
      841 ttgttactaa tagttactat tgagtttatt ttaaatttgt tcatcaaatc agatatactc
      901 agctctaatt tttcgcttgc atattttagt gctaaatcta tatctggaat cggatattcc
      961 caattatcca cacatatttc tatatcataa atatcctctg tattatgttc caaaaagtga
     1021 gatatactac taaaaaggca ggtttccact aattgtggat ataatatctg agcatattta
     1081 cgctctttgg tgatacctct atattctttt ctagtgtaat caccaaacgc cttataatta
     1141 tcgagattgt taattaatat ggagtcatcg agatttataa accttacatc tatttctatt
     1201 gggtatttaa ttaactcatt agaaatggta attgcttttg aaacattaat cttatcattt
     1261 tttaaaggct ttgatttaga aagccccatg attttatcac tctttttaaa tatatcttct
     1321 tcatcttcaa gttcataaat agctgataca cctacaaata tcctgttact ttttagatta
     1381 aaagtatggt taccatattc atcaaacaag actattattt ttttacttaa catattatta
     1441 atttaatttg gatgaactat aattaataat agatacttat ccttatggta gctgtaatat
     1501 tataatatta aaatttgtaa tgtagaaata tggatacaag tagcttaatt actaatttgg
     1561 aatttataga gaagaaaatt gatttgtctt tacctagaga tattcagaaa ctacctccca
     1621 ttaacttctt gattgtaact atattcaaat catgcattag tcactttaag tcaatcatat
     1681 tgttgttgaa atctaatatg tatcaagaag ccatggttat taatgattgc ctctttacgt
     1741 ctatcatatt tagatataaa accagaagat aggatacctt tgttatgcaa atgggactaa
     1801 aagacaattt taagtggctt aaaataagca agcacttaaa ttaggtatag aacctaatat
     1861 cgatgatgtt attgatacag ttcaggagag agaaaaagaa attaccaaat tcttaccaga
     1921 atccagctaa aaaacaaaaa attttccatc tataagtaaa atggcattag aactaaataa
     1981 gaatgaagct tacctaaatt accaaatttt aagcaaccat gttcacagct caatttcttc
     2041 aaatttatca agaataaaag agataaatta tgttaaatat ttcacaccag aaaaagcaaa
     2101 tgattaattt tcttacgcaa taacaacgga aacacttgag tggataatat gtgcttctgt
     2161 agcaatagga aacatccgtg aaatagataa attaaaagag tttaatgact ttggtgtatc
     2221 aggagaaggt attttgtcta atatacaaaa gttgaattaa tttgtttcta cactgtatct
     2281 aatatattaa taatacctta cttcatatat ataattcaca gttcatctga atatcgagct
     2341 tttgaactac agtattaatg ttattaaaaa atgaggactt gtttaacttt aacatcttcg
     2401 ttcggaatac gtccggtaag gtgtctatat caaagactat caagaaatca gaaaatatat
     2461 aaaacagtct ttatttgaag gattcaaact aattttattt tttattgaaa gattatttgt
     2521 cttttatctg tttacagtca ctgccatccc aattaccttt gataatgttt acatgcccca
     2581 gtcttgttac acttacctga ccgcaccttt tgaatttcgt gccatcttta ggacttataa
     2641 cacatgaaga ttctgttgca cttgaagggt ctaaagtcag agatccgtcc ggttttatca
     2701 caaaaggatt tgagggcaca atagtgatat caatatattt ttctaacttt aacatctggc
     2761 tagatatctc ggtccagtta gaggaaaggc tgttgctatt tcccctctcc cgttttatct
     2821 caattgtatc ttcatcatca gatattgtca tggaaagtct atattctact ccatccctgg
     2881 cagccttgag cttgcttatc tgcacttccc ccgaaaccgc atttgttagc tgatctatat
     2941 ctcttctctg gaaaaattga gaagaaaatg agcctgctat tactattaac agacttataa
     3001 tccctacaat agttactacc tcaatgagtg tgaatcctct tattgtagat ttagatatat
     3061 acataatatt ttcatcattg ccttaatact caaaaatgaa gatctaaata agctcctccc
     3121 aatacaaagt ttgtgtacgc tgtgggaaat ccgcaatcgt aataacaata aatgacgcac
     3181 cactatcacc gccgtcacca ctaactcctc ctgtaaatgc tatagtcata accagagatc
     3241 cgcgttcacc aaatgtaagt gatgcaggcg taggcatacc aaccccaaca cttttaccag
     3301 cagcagctgc tttttgtgtg tcactgcctg aaataatttt gtctgatcct ggaattctcg
     3361 caagagcaga ctcacccgtc agataatcaa gcgcaaatat tcttcctact cccggacctg
     3421 ccgaacagga attatcaagc agtgcatcag gttcaaaagt agaaaataat agtatcctgt
     3481 ttgcgacaat gggtcttcca atcccctttt cacctagcgt attactacca ccagggaaat
     3541 ttactaggaa ttcaccatta gcatttattg cagtctgatc aacagataca gcttcgaact
     3601 gattggctac tccgctctgc gcactaagat ttacaaccaa gtctcttgac gtgagatact
     3661 cttcagtctt tacctgagaa gcctgtacag ctgttcctcc ggcaagaaaa gcgggtataa
     3721 aatcatcgat aaagttataa agcttaccga atttctcatt atcagttggt tcccttctgt
     3781 ctccggtttg aaatatcaca tgctttcttc caatgtcgtc aatcaccacc gaagggggga
     3841 agtatatcgg gaataaagat ggcactgcag atgcatctgt tgtaacaact attgggtcac
     3901 taaattcatc catttcgtca actagacagt tcgcatcgct gcctgcgcag aacgctaccg
     3961 atcctgtcca gtcaggcgcg ctaataccag tatcaagaga tttaagagca agatcagaac
     4021 ttgtagcctc tgaatcggtt tcaatcggca tagacacatc aaatctccac atcctgccgc
     4081 atgtatctcc cacataggct acatccatat aaccgtctgc attcagatcg aatactccaa
     4141 cttcgctggc tacttcacat accatcaaat tggagtttgt aagcgtacta tctgatggat
     4201 ggaacttaaa tataatctcg ccagttgct
//
LOCUS       DBNGSV010000063         1899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1466637, whole genome shotgun sequence.
ACCESSION   DBNGSV010000063 DBNGSV010000000
VERSION     DBNGSV010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1466637"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC159U_02435"
     CDS             <1..311
                     /locus_tag="AC159U_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335944.1"
                     /translation="MADLGIQVKGVEWSVAFGILGNSHLIISVRNVGYVKSAGRLVRE
                     MFTEIGSAGGHRSAAKAVIPLSKMRKILGRASQVHIKKWITKQFSEALKDVPDLEKEK
                     "
     gene            462..1109
                     /locus_tag="AC159U_02440"
     CDS             462..1109
                     /locus_tag="AC159U_02440"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3335945.1"
                     /translation="MQHTEKFKYKLSQILSFIEDNNKKVFATMLKAFIVAALILSIIT
                     CISFWNKLGQKDSNFANTNEKISRQKEQIESLENQLYKTQQLISNLDEIILEQQDILD
                     FLQTTTLSTISLKNYQNNFVIHGKIFLDKANKKGVLIGKNLDVLQQGQIYQIWAVFKI
                     NPKSIGTFKSDSDGNALLKLEYIPNPNKIKRFIITKEIGTGSEEPTGDVYLLGGL"
     gene            complement(1110..>1899)
                     /locus_tag="AC159U_02445"
     CDS             complement(1110..>1899)
                     /locus_tag="AC159U_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR3335946.1"
                     /translation="IVLCVATGFVIIGGIKSIAKVTSAIVPFMIILYLIGAFISLIIN
                     FEQIPHAIYLIFHHAFNPTAASGGFAGALIQQTLRMGVARGIFSNESGLGSSPIAAAA
                     AKTANPVRQALVSMSQTFIDTIVVCTFTGLVIISSGLWNSGETGATLSALAFEKGVPN
                     NLGADMVSVCLAFFAYSTLLGWSYYGEKCIEFILKERVVIPYRIIFTVFVLVGAVIKL
                     DIVWTFADIMNGLMAFPNLVGLLLLSRVVVEETKLYLAAELGKK"
BASE COUNT          671 a          359 c          331 g          538 t
ORIGIN      
        1 aaatggctga cctcggaatt caggtaaaag gcgttgagtg gtcggtagca tttggaatat
       61 tgggaaactc tcatcttata atttcagtaa gaaacgtggg atatgttaaa agcgcaggaa
      121 gacttgtaag ggaaatgttt actgaaattg gcagcgctgg tgggcacaga tccgctgcaa
      181 aagcagttat accactatca aaaatgagaa aaatacttgg aagagcttct caggtacaca
      241 ttaaaaaatg gattacaaag cagttttctg aagctttaaa agatgttcct gatttagaaa
      301 aagagaaata agtaccttaa attaaatctc aaataatcgt agtaatatac tgtaaccatt
      361 acacttatta cttcataata tacttaaagt ttgttatatt gcttaattag ttataataac
      421 aaacattgaa attccataaa tccttataaa tctgattgaa tatgcaacat actgaaaaat
      481 ttaaatataa attatctcaa attctatcat ttattgaaga caacaataaa aaagtatttg
      541 caaccatgtt aaaggcattt attgttgctg cacttatact atccattatt acttgtatat
      601 cattttggaa taaactgggt caaaaggatt ctaattttgc aaatacaaat gaaaaaatat
      661 cccgccagaa agaacagata gaatcattag aaaaccagtt atataagacc cagcagctta
      721 tcagtaatct ggacgaaatt atcctggagc agcaggatat tcttgatttt ttacaaacca
      781 ctacactatc cacaataagt cttaagaatt atcaaaacaa ttttgtaatt cacggtaaga
      841 tctttttaga taaggccaat aaaaaaggcg tacttatagg caaaaactta gatgttttac
      901 aacagggtca aatatatcag atatgggctg tttttaagat caaccccaaa agcatcggca
      961 cattcaaatc agactcagac ggcaatgcgc ttctgaaatt agaatatatt ccaaacccaa
     1021 ataagattaa gcgttttata ataacaaaag agattggtac gggaagtgaa gagcccaccg
     1081 gggatgtgta tcttctcggc ggtctttagc tatttcttac ccagctctgc agcaagatag
     1141 agctttgttt cttctacaac aactctagat aacaacagga gccctacaag gtttggaaaa
     1201 gccataagcc cgttcataat atctgcaaaa gtccagacta tatcaagctt tataacggca
     1261 cctactaaaa caaacaccgt aaaaatgatc ctgtaaggga taacgactct ttcttttaaa
     1321 ataaactcta tgcacttttc tccataatag ctccatccaa gaagcgtaga atacgcaaaa
     1381 aacgcaaggc atacggacac catatcggcg cctaaattat ttggaacgcc tttttcaaag
     1441 gccaatgccg aaagtgtcgc tcccgtttct ccgctattcc aaagccccga tgaaatgatt
     1501 acaagtcctg tgaaagtgca gaccactata gtatcaataa aagtctggct cattgatacc
     1561 agcgcctgcc gtacgggatt agcagttttt gctgctgctg ctgcaattgg ggagctacca
     1621 agccccgatt catttgagaa aatccctctg gccactccca ttctaagggt ttgctgtatt
     1681 aaagctcccg caaatcctcc tgaggccgca gtgggattaa atgcatgatg aaatatcaga
     1741 taaatagcgt gtggaatttg ttcgaagttg ataataaggg aaataaaagc gcctattaaa
     1801 tatagtatta tcataaatgg tacgattgcg cttgttactt ttgctatact ctttatacca
     1861 ccaataatta caaatcctgt cgcaacacaa agaactatc
//
LOCUS       DBNGSV010000064         4614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1328255, whole genome shotgun sequence.
ACCESSION   DBNGSV010000064 DBNGSV010000000
VERSION     DBNGSV010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1328255"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="AC159U_02450"
     CDS             <1..437
                     /locus_tag="AC159U_02450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR3335947.1"
                     /translation="IRLYVTFFFLGNSPIAPGTAGTLGAILLLYFITNLSLPLYIALI
                     IILTAVSIYMSTLAVNIYKKKDPSHVVIDEVCGYLFTMILIPFTWVNVILGFFLFRAL
                     DITKPYPIRKLEKLKDGYGIVLDDVLAGIYANIIMQIFIRVY"
     gene            444..1685
                     /locus_tag="AC159U_02455"
     CDS             444..1685
                     /locus_tag="AC159U_02455"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR3335948.1"
                     /translation="MNIEIITTGNELLSGLTLDTNFHWASRLLADSGFEVKFHSTVGD
                     YEDDILQAFGNANARADVIIVTGGLGPTEDDLTAGVAAKYFGTKLCENPQALEMIKKI
                     FARRKRKLLEINKKPSYLPEGCKIINNNVGTAPGFYYEYDKKIFYFLPGVPKEFKSMM
                     QEFVLADINIRFSDNEKTKTLLLRTFGLKESELALKIQDLETEGVYLGYRSHFPEVHL
                     RLVAKGKSEEQINTKIESLLKKIKSEIGQYIYAESDTTMEEVVGGLLTEHSMSISLAE
                     SCTGGLVSHRITNVPGSSEYFNRSIICYSNESKINDLGVSRLLIENHGAVSTHVAEAM
                     AKGVLDLAGTDIGVGITGIAGPGGGSTEKPVGTVHISLACKNGDIISDKFSYHGDREQ
                     IKLITSTQSLDMIRKFLKNYV"
     gene            1709..2755
                     /gene="recA"
                     /locus_tag="AC159U_02460"
     CDS             1709..2755
                     /gene="recA"
                     /locus_tag="AC159U_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3335949.1"
                     /translation="MQETADKKSLEEKEKTIELAISSIEKQFGKGSIMRLGADAIIPE
                     ISAVSTGSIGLDLSLGVGGIPRGRITEVYGPESSGKTTIALHVIAEAQKLGGVAAFID
                     AEHALDPAYASRLGVKVDDLLVSQPDFGEQALEIVDTLVRSGAIDVIVLDSVAALTPR
                     VEIEGEMGDAHVGVQARLMSQALRKITATVNRSKTIVIFINQIRMKIGVPSYMNPETT
                     TGGNALKFYSSIRLEIRRIGSIKDGDNITGNRVRVKIVKNKVAPPFKDAEFDIVFGEG
                     ISYTGELIDIGVKHNVVEKSGTWFSYDGERLGQGRENARKFLIENPQLMEKLIVEIYE
                     KSGLSTKPKSSEDD"
     gene            2756..3883
                     /locus_tag="AC159U_02465"
     CDS             2756..3883
                     /locus_tag="AC159U_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3335950.1"
                     /translation="MAILKIDDLLKAALKVGASDIHLKTDSVPVFRVNGRLHFMKEAP
                     VINAEIMKSLYSSVMNKNQIERFERTFDIDLAYNIPDVSRFRVNVYQQRGETSMAIRA
                     IPYNILSFDDLNLPPILDKISMEDRGLVIVTGTTGSGKSTSLAAMIDNINTKKASHII
                     SIEDPLEYTHTDKNSYINQREVGIDTLSFSNAMRASLREDPDVILIGEMRDLESIEIA
                     TAAAETGHLVFTTLHTLDAQETINRMLAVFPSSQQNQIRYQLSQVLKAIISQRLMPQA
                     DGKGRVPAVEVLLATSRIKDCIADPTKTSEIRQAIEEGHLHYGMQTFDQCLYNLFRQK
                     LITYEEAMRQATNKDDLDLRIKGISSGSASLGRMDEPMQTQ"
     gene            3928..>4614
                     /gene="tsaB"
                     /locus_tag="AC159U_02470"
     CDS             3928..>4614
                     /gene="tsaB"
                     /locus_tag="AC159U_02470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3335951.1"
                     /translation="MIALGIETSSLCGSVAVAEDDKVLGELFFNSGLKHSTQIIPSID
                     KLLCTIGLQKSDLQGIAVSSGPGSYTSLRVGISAAKGIAYSLGLPLIGVSTLKCVAFN
                     SLITPFPICSIIDAKKGQLYSAFFRYNNNELEIIGEGDKIVSVDDICSSIEQKNEKIV
                     LIGDGLNLNKLTFMDRINGLVLFAPGHLCSPRASSCVLLGVDSFKKAKKDEVNTLVPR
                     YVREAHAELID"
BASE COUNT         1493 a          760 c         1032 g         1329 t
ORIGIN      
        1 gaattaggct gtatgtaaca ttttttttcc taggcaattc tcccatagcg ccgggaaccg
       61 caggtaccct tggtgccata ttacttctat actttataac taacctttcg ctgcctttat
      121 atatagcact tatcatcatt cttacagctg tttctattta tatgtccact cttgctgtaa
      181 atatttataa gaaaaaagat ccttcgcatg ttgtgataga tgaggtctgt ggatatcttt
      241 ttactatgat cctgatccca tttacatggg taaatgtaat tttaggattc tttttgttca
      301 gggcgcttga tattacaaag ccgtatccaa taagaaaact cgagaaatta aaagacggct
      361 atggaattgt gctggatgat gtgcttgcag gaatttatgc aaatataata atgcaaatat
      421 ttatcagggt ttattaaata cttatgaaca tagaaataat caccactgga aatgagcttc
      481 taagcggtct tacgctagat acaaattttc actgggcatc cagactcctt gcagacagcg
      541 gatttgaggt gaaatttcat agtactgttg gtgactacga agacgatata ctgcaggctt
      601 ttggaaatgc caatgccagg gcggacgtaa taattgtcac tggaggttta ggacccactg
      661 aagacgatct aactgccggg gtagctgcca agtacttcgg tacaaagctt tgtgaaaacc
      721 cccaagcttt ggaaatgata aagaaaatat ttgcaaggcg aaaaaggaag cttctggaaa
      781 taaacaaaaa accttcttat ttgcccgagg gatgcaaaat tataaacaat aacgttggta
      841 cagcgccagg gttttactat gagtatgata aaaaaatatt ttatttcctt ccgggtgtac
      901 caaaagagtt taaatctatg atgcaggagt ttgttcttgc agatataaat atcaggtttt
      961 cagataatga aaaaaccaaa acactgctcc tgagaacatt tggtctaaaa gaatcggagt
     1021 tagctctaaa aattcaagat ttggaaactg aaggtgtata cctgggatat agatcacatt
     1081 ttcccgaggt tcatttgaga cttgttgcaa aaggaaaatc tgaagagcaa ataaatacaa
     1141 agatagaaag tttactaaaa aaaataaaat cagaaatagg tcaatatata tacgcagagt
     1201 cagacacaac gatggaagag gttgttggag gtcttttaac agagcacagc atgagtatat
     1261 cgcttgccga atcttgtaca ggcgggcttg tttcacatag aatcacaaat gtgccgggaa
     1321 gctcagaata ttttaacagg tcgattattt gttatagcaa cgaatcgaag ataaatgacc
     1381 tcggtgtaag caggctgctc atagaaaatc atggagcggt gagcactcat gtagcggagg
     1441 ccatggcaaa aggagtattg gatttagccg gcacggatat aggcgttgga atcacaggaa
     1501 ttgcaggccc tggaggggga agcacagaaa aaccagtggg tacggtgcat atttcgcttg
     1561 catgtaaaaa tggtgatata atttctgata aattcagtta tcatggtgat agggaacaga
     1621 taaagcttat aacatctaca caaagtctgg atatgattag gaagttctta aaaaattatg
     1681 tataatcaat tacttatagg gagttaaaat gcaggaaaca gcagataaaa agagtttgga
     1741 agaaaaggaa aaaaccatag agcttgccat ttcttctata gaaaagcagt ttggcaaggg
     1801 ttcaataatg cgtctaggcg cagatgcaat aattccggaa atctcggcgg tctctacggg
     1861 ttcgatcggg cttgatctat ctcttggtgt tggtggtata ccaagaggca gaataacaga
     1921 agtgtacggg cctgaatctt caggaaagac tacaattgct cttcatgtaa ttgccgaagc
     1981 tcaaaaactg ggtggtgttg cagcatttat tgatgctgag catgccttag accctgctta
     2041 tgcttcgaga ttaggtgtta aagtggacga cctgcttgta tctcagccag attttggaga
     2101 gcaggctttg gagattgttg atacgcttgt aagaagcggt gctatagatg ttattgtgct
     2161 tgattcagtt gcagctctaa ccccgagagt agaaatagaa ggagagatgg gtgatgctca
     2221 tgtgggtgtg caggcaaggc tcatgtctca ggctctaaga aaaattacag ctactgtaaa
     2281 cagatctaaa acgattgtta tatttataaa ccaaataaga atgaaaattg gtgttccatc
     2341 ctatatgaac cctgaaacca cgactggcgg aaatgcgctt aagttttatt cttctatcag
     2401 gcttgagata agaagaatag gttcaataaa agacggagac aatattaccg gtaatagggt
     2461 aagggttaaa atagttaaaa ataaggttgc tccgccgttt aaggatgcgg agtttgatat
     2521 agtttttgga gaaggaatat cttataccgg tgagctcatt gatattggag ttaagcataa
     2581 tgtggtagaa aaaagcggta catggttttc ttatgatggt gagcgccttg gtcagggtag
     2641 ggaaaatgct cgaaaatttt taatagaaaa cccccaatta atggaaaaat tgatagtaga
     2701 aatatatgaa aagagtggtt tatctacaaa gcctaaaagc tcggaggatg attaaatggc
     2761 tatattgaaa attgatgatt tattgaaggc agcacttaag gtaggtgcat cggatattca
     2821 ccttaaaacc gatagtgttc ctgtgttcag ggtaaacggt aggcttcatt tcatgaaaga
     2881 agcaccggtt ataaacgcag agatcatgaa gagtctatat tcttctgtaa tgaataaaaa
     2941 tcagatagaa agatttgaaa gaacttttga tattgacctt gcatataata ttcccgatgt
     3001 ttcacgtttc agggtaaatg tttaccagca gcgcggagaa acttctatgg ctattagggc
     3061 tatcccttat aacatcttat cattcgatga tttaaacctc ccgccaatac ttgataaaat
     3121 aagtatggaa gacagggggc tagttatcgt aactggtaca acaggtagtg gaaaatctac
     3181 ttctcttgca gccatgatag acaatattaa tacaaaaaag gcatcgcata taattagtat
     3241 tgaagatcct ctggaataca cacacacgga caaaaatagc tatattaacc agcgcgaagt
     3301 cggaattgat actctttcat tctcaaatgc aatgagagca tctcttcgtg aagatccgga
     3361 cgttattctc attggtgaga tgcgtgactt ggaatcaatc gaaattgcta ctgctgctgc
     3421 agaaacaggt caccttgtgt ttactacgct tcatacactg gatgctcagg aaaccattaa
     3481 ccgtatgctt gctgtatttc catcgagtca gcaaaatcag atcagatatc agctctcaca
     3541 ggtgttaaaa gcaattatat ctcagagact catgcctcag gcagatggaa agggcagggt
     3601 tcccgcggtt gaagtcctgc tggcaacttc aagaatcaaa gactgtattg ccgatcctac
     3661 aaaaacaagt gaaatcaggc aggccattga agagggacac ctgcattatg gaatgcagac
     3721 attcgatcag tgcctttata atcttttcag acagaagctc attacatatg aagaagccat
     3781 gaggcaggct actaacaaag atgaccttga cctcagaatc aaaggtatta gctcgggatc
     3841 tgcttctctt ggaagaatgg atgagcctat gcagacacaa taagcattct gttggcattg
     3901 atattattta ggttgaattt ttagttaatg atagcactgg gaatagaaac atcatcttta
     3961 tgcggcagtg tggctgtagc agaagatgat aaagtattag gggagctttt cttcaattcc
     4021 gggttaaaac attctacaca gattattcct tctatagata agttgctttg tacaataggg
     4081 cttcaaaaat ctgacctgca gggaattgca gtttcttcgg ggcccggttc ttatacctct
     4141 cttagggttg ggatttcagc tgccaagggt atagcatatt ctctcggttt accccttata
     4201 ggggtatcaa ctctaaagtg tgtggctttt aattcactaa ttacgccatt tccaatctgc
     4261 agtattatag atgccaaaaa aggacagtta tattctgcat tttttagata taataataac
     4321 gaactggaga ttataggtga aggggataaa attgtaagtg tagatgatat atgcagtagt
     4381 atagagcaaa aaaatgaaaa aattgtgtta attggtgacg gattaaattt gaataaatta
     4441 acttttatgg atagaataaa cggtcttgtg ctttttgcac cagggcactt gtgtagtccg
     4501 cgtgcatcaa gctgtgtatt attgggtgtt gatagtttta aaaaggcaaa aaaggatgaa
     4561 gttaatacgc ttgttcctag atatgtaaga gaggctcatg ccgaattaat agat
//
LOCUS       DBNGSV010000065         2262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1813183, whole genome shotgun sequence.
ACCESSION   DBNGSV010000065 DBNGSV010000000
VERSION     DBNGSV010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1813183"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2262)
                     /locus_tag="AC159U_02475"
     CDS             complement(<1..>2262)
                     /locus_tag="AC159U_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3335952.1"
                     /translation="DDVGDKGELSLKFKIPEEDVIYGTLNVESSVRDDRGKYIASQAR
                     ADYIAVDRLVGLKSTRWMYDEDKPANVNYIVVDEKGKPAEGTKVNIVIERLETKAARI
                     KGAGNAYVTNYVDEWIPSGTCKGVSAKEALSCEFVPSDPGTYKLTATIADTKNKKHST
                     EYRIWVAGKGRVLWREPADNSIQIIPEKESYNIGDTARYLIKNPYPDSYALISIERFG
                     VMKHWVQRLQGSTPVVEFPVDKDYLPGFYFSVAVLSPRVETPPPAFGKVDLGKPAFKM
                     GYVKVPVKDAYKQIDIKIKTDKEVYKPRNKVKALIHVEPKNKDNSEPIEIAVAVLDEA
                     VLDLIQGGKNYFDPYGGFYKLENLDVVNYSLLSRLVGRQKFEKKGANPGGDGGASISM
                     RSIFKFVSYWNPSLKPDQDGNAEIEFTLPDNLTGWRIFAFAVTPSDRMGLGDTNIKVN
                     RPTEVRAVMPNQVTEGDSFIAGFSVMNRTDKDRTIKVSINASGDIKSGLTHEASVDLK
                     PYKRTTVYMPVESSRVDESRDVNKGIISFKVKANDKIDGDGIVHKLNVNKSRSFATAA
                     NYGTTTGANVSESILFPKDIYSDVGDISVTLAPSVIGNVQGAFRYIGDYPYICWEQKL
                     TKGVMASHFKNLKSYMPDDLKWEESGKLPQETLDQAANYQAPNGGMVYFIPADGYVSP
                     YLSAYTALAFNWLRESGYKIPQEVENKLHGYLKNLLKKDVLPTFYSRGMSSTVKVVAL
                     AALSEHGKVTLNDL"
BASE COUNT          571 a          544 c          407 g          740 t
ORIGIN      
        1 taaatcattt agggttacct tcccatgctc tgaaagtgct gctagcgcaa caacctttac
       61 ggttgatgac atacctcgtg agtaaaatgt tggaagcacg tcttttttga gcaggttctt
      121 taagtagccg tgcagcttat tttctacttc ttgcggaatt ttatacccgc tttctctaag
      181 ccagttaaat gcgagtgcgg tataggcgct taagtaagga cttacatatc catcagcagg
      241 tatgaagtat accatgcctc catttggagc ttgatagttt gcagcctgat ccaaggtttc
      301 ctggggcaat tttccacttt cttcccactt taaatcatcc ggcatgtagg attttagatt
      361 tttaaaatgt gaagccatta caccctttgt aagcttttgc tcccagcaga tataggggta
      421 gtcccctatg tatctgaatg cgccctgcac attgcctatg accgaaggag caagcgttac
      481 ggaaatatca ccaacatcag aataaatatc ttttggaaat agaattgatt cagaaacatt
      541 tgccccggtg gtcgttccat agttagcggc agtcgcaaac gagcggctct tgtttacatt
      601 cagtttgtgc acaattccat caccatctat cttatcattc gctttaacct taaagcttat
      661 tattccttta tttacatctc tactttcatc gactctcgac gattctaccg gcatgtacac
      721 agttgttcgt ttataaggtt ttagatcaac agatgcctcg tgtgtcaaac cagacttaat
      781 atctccgctg gcatttatag ataccttaat agtacggtct ttgtccgtgc ggttcatcac
      841 actaaaacct gcaatgaaac tgtcaccctc ggtgacttga ttgggcatta cagctcttac
      901 ctcagttggc ctgtttactt ttatatttgt atcaccaagt cccatcctgt ctgatggggt
      961 aacggcaaag gcaaatattc gccaacctgt aagattatcg ggaagcgtaa actctatttc
     1021 tgcgttacca tcctgatctg gttttaacga cgggttccag tagcttacaa atttaaagat
     1081 agacctcatg ctaattgaag cccctccatc tccacctgga ttggctcctt ttttctcaaa
     1141 tttttgccta ccaaccaacc tgctaagcag actataatta accacgtcca gattttcgag
     1201 tttataaaaa cccccatacg gatcaaaata attcttgcca ccctgaatta gatcaagtac
     1261 tgcttcatcc aaaactgcaa cagctatttc aataggttct gaattatctt tatttttcgg
     1321 ctctacatga attagtgctt taactttgtt tctgggtttg taaacctcct tatctgtttt
     1381 aatcttgata tcgatctgtt tatacgcatc ctttacaggc acctttacat agcccatctt
     1441 gaaagcgggc tttccaagat caaccttccc aaatgcagga ggaggtgtct ccaccctggg
     1501 tgatagaaca gcaaccgaaa aatagaaccc cggcaggtag tccttatcga ctgggaattc
     1561 aactacgggg gtgctgccct gcagtctttg tacccagtgt ttcatgactc caaaacgctc
     1621 tatactaatt agcgcatatg aatcgggata gggattttta atgaggtacc gggctgtatc
     1681 tcctatatta tatgattctt tttcaggaat tatctgtatg gaattatcgg caggctctct
     1741 ccataacaca cgccccttcc cggctaccca tattctatac tcagtagaat gttttttgtt
     1801 ttttgtatct gctatggttg ctgtaagttt atacgtgccc ggatcacttg gtacaaattc
     1861 acaactgagg gcctcttttg ctgatacgcc cttgcatgtc cccgatggaa tccactcatc
     1921 tacataatta gttacatatg catttcccgc tccttttatc cttgcagcct ttgtttccaa
     1981 gcgttctata actatattta ctttagtacc ttctgcaggc ttaccttttt catccacgac
     2041 tatgtaatta acattagcag gcttatcttc atcatacatc cagcgggtac ttttaagacc
     2101 cacaagcctg tcaacagcaa tgtaatctgc ccttgcctgt gatgctatgt atttacctct
     2161 atcgtcgcgc accgagcttt ctacattaag cgtgccgtat attacgtctt cttcgggtat
     2221 tttgaattta agagaaagct cgcctttatc ccccacatca tc
//
LOCUS       DBNGSV010000066         1982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1536320, whole genome shotgun sequence.
ACCESSION   DBNGSV010000066 DBNGSV010000000
VERSION     DBNGSV010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1536320"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="AC159U_02480"
     CDS             <1..1657
                     /locus_tag="AC159U_02480"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3335953.1"
                     /translation="KKIVNFIKKARANRADIVCFPELSITGYPPEDLLLNGSFIEQNI
                     KALDEITKHTKSIIVIVGFADRRNGLLNAAAIMSNGKLLDIYHKRHLPNYGVFDEKRY
                     FKKASRTPVYELDKIRFGVNICEDIWVKGDPTRSQSLAGGADIVINISSSPYYASKVQ
                     SREQMLQERARDYGCYVVFCNLIGGQDELVFDGHSCVIDNGGNIISRGKGFKEQLLFT
                     DIDDKQIAGLRENKVKLKDKSRVSQVDIVKLAGKLKKKTQNNINNKVSVFYSQYEEVF
                     KALVLGTKDYINKNGFKKVVIGLSGGIDSSLVCVIAKEALGAKNVTGVLMPSQYSSAG
                     SITDSEKLSHNLGIETLTVPIKKLFDQYNKSLSEIFIGTTEGITEENLQPRIRGNILM
                     ALSNKFGWLVLTTANKSETSVGYSTLYGDMAGGFAVIKDVPKTLVYKLCEYYNESKKS
                     RIIPQSVLEKPPSAELRPDQKDTDSLPEYDILDPILKLYIEDDLGIDQIAELGYNKKL
                     VHRVIKLVDINEYKRRQAAPGVKITPRAFGKDRRFPITNRFHN"
     gene            1695..>1982
                     /locus_tag="AC159U_02485"
     CDS             1695..>1982
                     /locus_tag="AC159U_02485"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908509.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3335954.1"
                     /translation="MNFADPKEQLEIISKGSEEIISEQELLKKLEKSSKENTPLRIKA
                     GFDPTAPDIHLGHCVLLKKLRDFQDLGHTVVLIIGDFTAMIGDPSGRSDTRP"
BASE COUNT          704 a          330 c          424 g          524 t
ORIGIN      
        1 gaaaaagatt gttaatttta taaaaaaggc cagggctaac cgtgcagata tagtttgctt
       61 tcccgagctt tctataacag gctacccgcc tgaagatttg ctcctaaacg ggtcgtttat
      121 tgaacaaaac attaaggcgc ttgatgagat aacaaaacat acaaaatcaa taatcgtgat
      181 agtaggattt gctgatagaa gaaatggtct attaaacgca gcagcaataa tgagtaacgg
      241 aaaacttctt gatatttacc acaaacgcca tctgccaaat tatggagtct tcgatgaaaa
      301 aagatatttc aaaaaagctt caagaacgcc ggtatatgaa ttggataaga taaggtttgg
      361 agtgaatatc tgtgaagata tatgggtcaa gggcgatcct acaagatcac agtctttagc
      421 cggcggcgcc gatattgtaa taaacatatc ctcatcccca tactatgctt ccaaggtgca
      481 gagcagggag cagatgctgc aggaaagagc cagggattat ggatgctatg tagtgttttg
      541 caacctcata ggaggtcagg atgagcttgt ttttgacggg catagctgtg taatagataa
      601 cggtggaaat attatttcac gcggaaaagg atttaaagaa cagctattgt ttacagatat
      661 cgatgataaa caaatagccg gactaagaga aaataaagta aaattaaaag ataaatccag
      721 ggtctctcaa gtagatattg ttaaattagc cggcaaatta aaaaagaaaa cacaaaataa
      781 tattaataat aaagtatctg ttttttattc tcagtatgaa gaagtattca aagcgcttgt
      841 tcttggtaca aaggattata ttaataaaaa tgggtttaaa aaagtagtta ttgggcttag
      901 cggcgggata gattcaagtc ttgtttgtgt tattgcaaaa gaggctctag gcgctaaaaa
      961 tgttacggga gtattaatgc cctcacaata tagttcggcg ggcagtatta cggattccga
     1021 gaagctttct cataacctgg gtattgaaac tctaactgtg cctatcaaaa aattattcga
     1081 tcaatacaat aaatcactat cagaaatttt cattggtacc acagaaggca taaccgaaga
     1141 aaatttgcag ccaaggataa gaggaaatat cctcatggct ctatcaaaca aattcggctg
     1201 gcttgtgctt actactgcta ataagagtga aacaagcgtt ggttacagca ccttatatgg
     1261 tgatatggca ggcggctttg ccgttataaa agatgtccct aaaacactgg tttataagct
     1321 ttgtgaatat tataatgagt ccaagaaaag caggattatc ccacagagcg tgctcgaaaa
     1381 accaccttca gccgagctca gaccggatca aaaagatact gattctcttc ccgagtatga
     1441 tattctcgat cccatcctta aattatatat agaggatgat cttggcatag atcagattgc
     1501 tgagcttgga tataataaaa aactagtaca tagggttata aaacttgtag acataaatga
     1561 gtataaaaga aggcaggcag cgcccggggt taagattacg ccaagggcat ttggaaagga
     1621 tagaaggttt cccattacaa acagatttca taattagtgc tttttcacca agaaaacgag
     1681 gagataaaag gtagatgaat tttgcagatc caaaagagca gctggaaata ataagcaaag
     1741 gttcagagga gataatatct gaacaggagt tactaaaaaa attagaaaag tcttcaaaag
     1801 agaatacacc cctgcgtata aaagccggtt tcgatccaac agcaccagat attcatctcg
     1861 gacactgcgt gcttctaaaa aaactaaggg attttcagga tttaggacac acggttgtac
     1921 taataatagg tgattttacg gcaatgatag gagatccttc gggaaggtct gacaccagac
     1981 ca
//
LOCUS       DBNGSV010000067         3500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1882710, whole genome shotgun sequence.
ACCESSION   DBNGSV010000067 DBNGSV010000000
VERSION     DBNGSV010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1882710"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            593..1039
                     /locus_tag="AC159U_02490"
     CDS             593..1039
                     /locus_tag="AC159U_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335955.1"
                     /translation="MKSIFVPNESFENKSEIDSINSYLYKNSQLLLEEIDDLTEIWII
                     GSKDEDFFKMQTIKAFEENRVLDPFDNMPVAPYKKIQHIAVINHKYGADSSLNTEIIY
                     ANADNDVTGFTQMVIHLLMKIYPKKRSRSDDSELNKSDRGDLALTG"
     gene            complement(1122..2090)
                     /locus_tag="AC159U_02495"
     CDS             complement(1122..2090)
                     /locus_tag="AC159U_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3335956.1"
                     /translation="MEYNNLGVSNLNVSKIGFDCWAVGGSWGDTDDKQSIATIRKALD
                     LGINFFDTADIYGFGHSETLLGKALGKKRKDVIIATKVGLAWDEHGCMFRASTKQHIT
                     HALEASLKRLGTDYIDLYQIHWPDTNTPFEVTMNVMDSLVKSGKVRYIGVSNFTVKQI
                     RECMKTRAIHSLQPPYNMLMRDAEKTLLSYCRRNGVGVIAYGPLAYGLLTGKFDAETT
                     FPDNDWRSGKLFPDPGDWQYHINIFQGKQFLRNLRIVEKLKKVAKKYDKTVGQLAIAW
                     VLSNPSISSALVGAKNIDQLEQNIGGQGWTIEKDDLNRINTILGRY"
     gene            complement(2188..2829)
                     /locus_tag="AC159U_02500"
     CDS             complement(2188..2829)
                     /locus_tag="AC159U_02500"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIR3335957.1"
                     /translation="MRVSEIFFSVQGEGIQIGLPTTFLRLFGCDLRCKWCDTMYAVEG
                     TEFNTLDIDNIIDKISNLECKRVCITGGEPLLQEKELIELTRILLDQDFTLVLETSGH
                     RAPPEIFWDDSCLISMDCKCPSSGMQDRMDFSLFEKLRGSDQLKFVITDDRDYQYAKQ
                     VLNNNQISSNIIFQPVYNSNTNWLYDKVLEERLDSARVLPQLHKLIWGEKRGV"
     gene            complement(2851..>3500)
                     /gene="queC"
                     /locus_tag="AC159U_02505"
     CDS             complement(2851..>3500)
                     /gene="queC"
                     /locus_tag="AC159U_02505"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR3335958.1"
                     /translation="GGIDSTTLLYKLINDGFKPVVLTFLYGQKHNKEIEYVINTCSLL
                     DVNNITVDITGIKDLLQGSALTDSKVDIPEVPETSEHFDTLKTTIVPNRNSIFLTLAV
                     AYAQSIGCNNIFFGAHFSDRGVYPDCRKEFVEAFEKSQILANDNKDLRVCAPFVDMDK
                     ADIVKQGNELGVQFEKTWSCYKGEEKHCGVCSSCRERKRAFIEANVTDPTVYKDQ"
BASE COUNT         1083 a          650 c          572 g         1195 t
ORIGIN      
        1 taaagcgaat gctatctgtt gttctgtaaa tcttgacttc ctcatggcaa aatacctcct
       61 gatttattgc attattttgc cagatttctc tacttttacc tggttcagtt tgcagggtga
      121 aggtcaagta ttattaggga aatacagatt ataatattac agattttatt aatgctctga
      181 ttaaaagatt gggtaatcaa ttccctaaaa acaaagttaa tataaaaaac aatagacaag
      241 aagataagaa tatcaaggaa caaaacagcc attattaaat acaaaagagc attataataa
      301 agattgtaac tataatttta tctgtactag atgtagagaa ttcacaatag taaggatggt
      361 ctaggtgctc atagtttact gaaatcatta tacaaaaata tccctatgta tgacaatatg
      421 cgttgcaacg tagcgtattt tgttattata tgtcaaattt ctgcatacga tctgccatat
      481 ttaagtacgt ttaagtctcc aatactcttg aatatttaat attatatcaa ttatggcata
      541 aaaattgctc tataataaat caaaagtatt gttaataatc tttggaggca caatgaaatc
      601 gatattcgta ccaaacgaaa gttttgagaa taaatcagag attgacagta ttaatagtta
      661 tttatacaaa aattctcaac tgcttttaga agaaatagac gatctgaccg aaatttggat
      721 aattggaagt aaggatgaag actttttcaa aatgcaaacc ataaaagcat ttgaagaaaa
      781 cagggttctt gatccatttg acaatatgcc tgtagcacca tataaaaaaa tacagcacat
      841 agcagttata aatcataaat atggtgctga ttccagttta aatacagaaa taatttatgc
      901 taacgcggac aatgatgtaa caggttttac tcagatggtt atacatctat taatgaaaat
      961 atatcctaaa aagagatcac gtagtgacga ttctgagtta aataaaagtg acaggggcga
     1021 tttggcgtta acaggttaat ttgattatag ctgcctgttt ttatcaaaca acatagtttc
     1081 taactcttaa acttctaaaa gagtacttta acaactatat atcagtatct tccgagtata
     1141 gtattaattc tgttcagatc atctttttct atagtccagc cctgtccacc tatgttttgt
     1201 tcgagttgat caatattttt agctcctaca agtgccgatg aaattgatgg gttagaaagg
     1261 acccaggcaa ttgctaactg gccgactgtt ttatcatatt tttttgccac cttttttagc
     1321 ttttcaacta ttcttaaatt tcttaaaaac tgtttaccct gaaaaatatt tatatgatac
     1381 tgccagtcac ccgggtctgg gaatagtttt ccagaacgcc agtcattatc aggaaaggtt
     1441 gtttcagcat caaatttgcc ggtcagtaga ccatacgcaa gaggtccgta tgcaataact
     1501 cccactccgt ttcttctaca gtatgaaaga agggttttct ctgcatctct cattagcata
     1561 ttgtacggtg gctgaaggga atgaattgcc cttgttttca tacactctct tatttgcttg
     1621 accgtgaaat ttgatactcc tatatatctc accttgccgg atttaacaag gctgtccata
     1681 acattcattg taacttcaaa cggagtgtta gtatccggcc agtgaatttg gtagaggtcg
     1741 atataatctg taccaagtct tttaaggctg gcttcaagcg cgtgggttat atgctgctta
     1801 gtggatgccc tgaacataca gccatgctcg tcccaggcaa gccctacttt tgtagcaata
     1861 attacatcct ttcttttttt gccaagtgct ttaccaagca gtgtttctga atgaccaaat
     1921 ccgtatatgt cggctgtgtc gaaaaaattg atacccagat ccagagcttt tctaatagtt
     1981 gctatggact gtttgtcatc agtatctccc cagcttccac ctacagccca gcagtcaaaa
     2041 cctattttgg aaacatttaa attagagacc ccaagattgt tgtattccat gatgtatgac
     2101 cccgttacaa agttgtttaa atagatatta tccttatatt ataaatggta tgtcaacccc
     2161 tatttaatga actggctaaa aaaagtgtta aactcctctt ttttctcccc atattaactt
     2221 atgaagctga ggtagaactc ttgcactgtc tagtctttcc tctagcactt tgtcgtataa
     2281 ccaatttgta ttagaattat aaactggctg gaaaataata tttgaagata tttgattatt
     2341 atttaatact tgttttgcat actgataatc cctatcatca gttattacaa actttagctg
     2401 atctgaacct cttagctttt caaaaagtga gaaatccatt ctatcctgca ttccggagct
     2461 tggacacttg cagtccatgc ttattaaaca actatcatcc cagaatatct ccggaggtgc
     2521 tctgtggccg cttgtttcaa gaactaaagt gaagtcctgg tctaaaagta ttctggttaa
     2581 ttctattagt tctttttcct gcaatagagg ctcgcctccg gtaatacata ctcttttaca
     2641 ttcaagatta ctaattttat ctatgatatt atctatatct agagtattaa attccgtacc
     2701 ctctacggca tacattgtat cacaccattt gcaccggagg tcacatccaa acagtcttaa
     2761 aaatgtagta ggaagtccaa tctgtattcc ttcaccctga actgaaaaaa atatttcgct
     2821 tactctcatt ttatgtatgt agttataatt tcactggtct ttataaactg tcgggtctgt
     2881 aacattggcc tcaataaaag ctctttttct ttccctgcag gagctgcata ctccacagtg
     2941 tttttcctca cctttatagc atgaccatgt tttttcaaat tgcacaccaa gttcattacc
     3001 ctgttttacg atatctgcct tgtccatatc tacaaatggt gcacaaactc ttaggtcttt
     3061 attatcattt gctaggatct ggcttttttc aaaagcctct acaaactctt ttctgcagtc
     3121 cggataaacg cctctatctg aaaaatgggc tccaaaaaat atattattac atccaatgga
     3181 ttgtgcatat gccacagcca gggtcaaaaa tatagagttt ctgtttggaa ctattgttgt
     3241 ttttaaagta tcaaaatgct cagaagtttc tggcacttcg gggatatcaa cttttgagtc
     3301 agttagtgct gagccctgta ataaatcttt aattcctgtg atatctacag taatgttgtt
     3361 tacgtctaat aaactgcatg tgttaataac atattcaatt tctttattgt gtttttgacc
     3421 atagagaaat gttagtacaa cgggcttgaa gccatcattt ataagcttat aaagtagtgt
     3481 agtactatct attcccccag
//
LOCUS       DBNGSV010000068         3788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_913894, whole genome shotgun sequence.
ACCESSION   DBNGSV010000068 DBNGSV010000000
VERSION     DBNGSV010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_913894"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC159U_02510"
     CDS             <1..530
                     /locus_tag="AC159U_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3335959.1"
                     /translation="TPQDIPIAEQQVTRLLRERHNINKNQVNGFHIKTQLFVIDRILT
                     VSRIMTILLGSIASISLIVGGIGIMNIMLVSVIERTKEIGIRMAVGAKQKDIMIHFVI
                     EALLLSIIGGTAGILLGIGILKIASSVTNLQTFVSTSAILIAFLFSAIVGVFFGYYPA
                     KKASKLNPIEALRYE"
     gene            complement(536..2416)
                     /locus_tag="AC159U_02515"
     CDS             complement(536..2416)
                     /locus_tag="AC159U_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR3335960.1"
                     /translation="MELFNRLIKVFILFQVVAILSCTVGDDTAPTDNTVITFNSNNKT
                     IEFIAPGDDGTTGLVTIYDLRYFDEFELTLLLGDTVDKVPFGTIQQVVQSMFADATQI
                     QGEEFPQPSGTAQSIFIPRLDISGETRYYFTMVSRDEVGNQSSPTNVIEATTGFVGSS
                     FINSSEGSCFGQSIAGVKIDEREDRSDDEFRDGDDIIIGDPCLDTVYVFFGGKGFGDR
                     DANSDGIFDLPSPDTADITIRGVPGTMFGASVEGIRDVAGDDSEEIVIGAPGFDSNRG
                     RVFIILGDRDGLPAVMDFNAGFTADITINGQAQGDNFGLNLLAAIGITGSGSDSLLIG
                     APTALSNRGKAYFYGENDLDDLNRDEPVSADTAKAIFTGEDPGDLFGTEITEVGIIDD
                     NDRSDFAISSPGAAKVYVFFVPSDVDDIDLSTDTSRVVIISGDTEDEFGAAIGGDGDY
                     DGDPEDDVNFGDISRSNNREDVLIGAPGFDGERGRVLFYSGFDIEDAFDLGTTPTASK
                     IISGLVPGGRFGAEIRDMGDLNPDLDPEDRSEGTILEFDDNNEDFLVSAPTDGTGKVF
                     LFLGSTNFADSQTTDDADIVFTSQSGAGNFGSVLQNLDDVSEDELNDIGIAENGGVNV
                     LF"
     gene            2612..>3788
                     /gene="eno"
                     /locus_tag="AC159U_02520"
     CDS             2612..>3788
                     /gene="eno"
                     /locus_tag="AC159U_02520"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3335961.1"
                     /translation="MASIISVKGREILDSRGNPTVEVDIYCDDGSLGRAAVPSGASTG
                     EHEALELRDKDKNRYLGKGVRKAVNNINKTIASKLIGHDVRNQSGIDSALLNLDKTAN
                     KSRLGANAILGVSLAAAKAAACSLDIPLYKYLGGAYANTLPVPLMNIINGGAHADNNL
                     DFQEFMIVPYGFKTFSEAIRAGVETFHHLKSLLKSNNYSTAVGDEGGFAPNLKSNEQA
                     IELVMQAIENAGYSTTDNIAIALDVASSEFYEKGKYIIGSKRFDKDAMIKMYEKLTEN
                     YEIISIEDPMYENDWKGWQELTSTIGQKVQLVGDDLFVTNTKRLKRGIDNNVANSILI
                     KVNQIGSLTETMQAIDMAKKAGYTNIISHRSGETEDSIIADLAVATNAGQIKTGSASR
                     "
BASE COUNT         1207 a          829 c          674 g         1078 t
ORIGIN      
        1 gaaccccgca ggatatacca atagctgaac agcaggtgac aaggcttcta agagagcgtc
       61 ataatataaa taaaaatcag gtaaacggat ttcatattaa aacccagctc tttgtaatcg
      121 acagaattct cactgtatca agaatcatga caattttgct tggaagcata gcatccatat
      181 cccttattgt aggtggaatt ggtattatga acatcatgct cgtgtcggtt atagaaagga
      241 caaaagagat tggaatcagg atggcagtgg gcgcaaagca aaaagatata atgattcatt
      301 ttgttattga agctctatta ctttcaatca tcggcggtac ggctggcata ctgctaggaa
      361 ttggaattct gaaaatagca agctcggtta caaacctgca gacctttgta tctacaagtg
      421 ctatactaat agcgttcttg ttttcagcaa ttgtaggtgt tttctttgga tactaccctg
      481 caaaaaaagc ttcaaagcta aatcccattg aagcacttag atatgaatga gcatgttaga
      541 aaagaacatt tactccgccg ttttcagcta tcccgatatc atttagctca tcttcactaa
      601 catcatctaa attttgcagt accgatccaa agtttcctgc ccctgattga ctagtaaaaa
      661 caatatccgc atcatcagta gtctgtgaat cagcaaaatt agtgctgcct agaaataaaa
      721 acactttgcc cgtgccgtct gttggagcgc taaccaaaaa atcttcgtta ttatcatcaa
      781 actctaaaat tgtaccctcg ctcctatctt ctggatcaag atcaggattt aaatcaccca
      841 tatcacgtat ttcagcgcca aatctgccgc ctggtaccag acccgatatt atctttgaag
      901 ctgtaggggt tgttccaaga tcaaatgcat cttcaatatc aaatcccgaa taaaacaata
      961 ccctgcccct ttctccatcg aatcccggtg ctccaataag tacatcttct ctgttgttac
     1021 ttcttgatat gtctccaaag ttaacatcat cctccggatc accgtcatag tccccatctc
     1081 cgccaattgc agctccaaac tcgtcttcag tatctccact aattattact actcttgaag
     1141 tatctgtaga cagatcaata tcatctacat ctgaaggaac aaaaaacaca taaacttttg
     1201 ctgcaccggg agaagaaatt gcaaaatcag acctgtcgtt gtcatcgatt attccaactt
     1261 cagtaatttc cgtaccaaac agatcgcctg gatcctcacc tgtaaagatg gccttagcgg
     1321 tatctgcact aaccggttca tctctgttta aatcatctaa atcattttct ccataaaaat
     1381 atgcctttcc tctatttgac agagcagtag gagctcctat taaaagacta tcagaaccag
     1441 aacctgtaat gcctatagca gctagcaggt taagcccgaa attatcaccc tgggcttgcc
     1501 catttattgt aatatctgct gtaaatccgg cgttaaaatc cataactgca ggcagcccgt
     1561 ctctgtctcc taatattata aacactctgc ctctgtttga atcaaacccc ggagcaccaa
     1621 taacaatttc ttctgaatcg tcacccgcaa catctcttat cccttccaca ctcgctccaa
     1681 acattgtccc cggtactcct cttatagtta tatcagcggt atcaggactg ggtagatcaa
     1741 atattccgtc tgaattcgca tctctgtctc caaaaccctt tccaccaaaa aacacataaa
     1801 ccgtatcaag gcacgggtca cctatgataa tatcgtcacc gtctctaaat tcatcatcac
     1861 ttctatcctc tctttcatca atcttaacac ctgctattga ctgtccaaaa cagctgcctt
     1921 cggaactatt aataaacgaa ctgccgacaa accctgtagt agcttcaatc acatttgtag
     1981 ggctgctctg gttacccact tcatctcttg aaaccatggt gaagtagtat ctagtttcac
     2041 cagaaatatc cagtctggga ataaagatac tttgtgccgt accacttggc tgaggaaatt
     2101 cctcaccctg tatctgtgtg gcatctgcaa acattgactg tacaacctgc tgtattgttc
     2161 caaaaggaac cttatccact gtatctccca gaagtagtgt aagctcaaac tcatcaaaat
     2221 atcttaagtc ataaatagta acaagtcccg tagtaccatc gtctccgggt gctatgaact
     2281 ctatagtttt gttatttgaa ttaaacgtta ttactgtatt atctgttggg gcagtatcat
     2341 caccaaccgt acatgacaaa atagccacta cctgaaaaag gataaatact tttattagtc
     2401 tattaaacaa ttccatatct cgcgcttcct taactaaatt aattgagaat tttacagaac
     2461 cctaaatgta acataaataa atacaaacat gtaataattt actatatcta tctctacaat
     2521 tagtatacct tagagtgtct attgatagtt ggcatatttt tgctattatg gttatctttg
     2581 aatttacatt atttttttgg aggcataaaa cgtggcatcg atcatttctg taaaaggtag
     2641 agaaatcctg gattccagag gaaatcctac tgttgaggtg gatatttact gcgatgatgg
     2701 ttcattggga agagcagctg taccatcggg agcctccaca ggcgagcatg aagcactgga
     2761 gttaagagac aaagataaaa acagatacct tggcaaaggg gttagaaaag ctgtaaacaa
     2821 tatcaataaa acaattgctt caaaactgat aggtcacgat gtaagaaatc agtcaggaat
     2881 tgactctgcg ttattaaacc ttgataaaac agccaacaaa tccagacttg gagctaacgc
     2941 tattcttgga gtttctcttg ctgcagccaa ggcagctgcc tgctctttgg atataccgct
     3001 gtataaatac ctcggcggag catatgccaa tacccttccc gtacccctta tgaacataat
     3061 aaacgggggt gctcatgcgg ataacaacct ggattttcag gagtttatga tagtaccata
     3121 cggatttaaa accttctctg aggcaattag agcaggggtg gagacttttc atcacctaaa
     3181 gagccttctc aaatcaaata actattccac agctgtagga gatgaaggcg gctttgctcc
     3241 aaatcttaaa tcaaacgaac aggcaattga gcttgtaatg caggctatag aaaatgccgg
     3301 gtatagcact accgataata ttgcaattgc cctggatgtg gcatctagtg aattttatga
     3361 aaaaggcaag tatatcatag gttcgaagcg ctttgataag gacgcaatga ttaagatgta
     3421 tgaaaaactt accgaaaatt atgagataat ttcaattgaa gacccaatgt atgaaaatga
     3481 ctggaagggt tggcaagaac ttacaagcac aattggacaa aaagtacagc ttgttggaga
     3541 cgatctgttt gttacaaata caaaaaggct aaaaagggga atagacaata atgtggccaa
     3601 ttcaatttta ataaaagtta atcagattgg ctctctaaca gagactatgc aggcaataga
     3661 catggccaaa aaagcaggat atacgaatat catatcccac aggtccggtg aaactgaaga
     3721 ttcaataata gccgatcttg ctgttgcaac taatgctgga cagattaaaa caggttcagc
     3781 atccagaa
//
LOCUS       DBNGSV010000069         1201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1467892, whole genome shotgun sequence.
ACCESSION   DBNGSV010000069 DBNGSV010000000
VERSION     DBNGSV010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1467892"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..>1201
                     /locus_tag="AC159U_02525"
     CDS             join(83..332,332..>1201)
                     /locus_tag="AC159U_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612266.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3335962.1"
                     /translation="MRKSRFTEQQIAFALKQAETGTRVSEVCRKMGVAEATFYNWKKK
                     YSGLGISELRRLKQLEEENRQLKKLVADLSLDKHMLQDVNSKKALKPAVKRSLAEYVD
                     RVYRVGMRRSCRVVNIGRSTYYYRSVRDDRALRKRIVEIAEARVRYGYKRIHILLRRE
                     GWHVNHKKVYRIYCEDGLNLRRKRPRRRVSAACREMRPVVSKIDQTWSMDFVSDSLFN
                     GRRIRALTIVDNFSRECLAIHADHSIKADKVVEVMEALKRFVGRVPERIQVDNGSEFI
                     SKALDKWAYENKVVLDFSRPGKPTDNAYIESFNGSFRDECLNTHWFLSLDDARKKIET
                     WRRDYNEYRPHSALDNMTPEEYVKEHKKPEISNYPGTAF"
BASE COUNT          387 a          203 c          325 g          286 t
ORIGIN      
        1 ttgaccttca ccccgtaaac tgaaccagtc aaaagtagag aaatctggca aaataacgta
       61 ataaatcagg aggtattttg ccatgaggaa gtcaagattt acagaacaac agatagcttt
      121 tgctttaaag caggcggaga cagggacaag ggtatccgag gtatgcagga agatgggggt
      181 agcagaggct acattctata actggaagaa gaaatacagt ggcctgggaa taagtgagct
      241 caggcgattg aagcagctcg aggaggagaa caggcagctt aagaaactag tagctgactt
      301 aagtctggac aagcatatgc ttcaggatgt tatagcaaaa aagctctgaa gccagccgtt
      361 aagcggagct tagctgaata tgtggatagg gtttaccgtg tggggatgag aagatcatgc
      421 agggttgtaa atattggcag gtctacttat tactaccgtt cagtcaggga tgacagagct
      481 cttaggaagc gtattgttga gattgcagag gcaagggtac ggtacggata caaacgcatc
      541 cacatactgc tcaggcgaga gggctggcat gttaatcata agaaggtgta caggatatac
      601 tgtgaagacg gacttaatct aagaaggaag cgtccgaggc gaagagtatc cgctgcctgc
      661 cgggaaatga gacctgttgt atctaaaata gatcagacat ggtcaatgga ttttgtgtcc
      721 gatagtctgt ttaatggcag gcggataagg gcattaacta tagtggataa ttttagtcgt
      781 gagtgtcttg ctatccacgc cgatcactct atcaaagcgg acaaggtagt ggaagtaatg
      841 gaagcattga aacgttttgt gggaagagtc cctgagagga tacaagtaga taatggcagt
      901 gagttcatat ccaaagcgct agataagtgg gcatatgaga acaaagtagt gcttgacttc
      961 tcaagaccag gtaaacctac tgataatgcc tatatcgagt cattcaatgg aagttttcgg
     1021 gatgagtgtt taaacactca ttggtttctt tcacttgacg acgcaagaaa aaagatagaa
     1081 acttggagga gagattacaa tgagtacaga ccccattcgg cactagataa catgacgccg
     1141 gaggagtatg ttaaagaaca caaaaagccg gaaatctcta attatcctgg tacagctttt
     1201 g
//
LOCUS       DBNGSV010000070         8577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1191071, whole genome shotgun sequence.
ACCESSION   DBNGSV010000070 DBNGSV010000000
VERSION     DBNGSV010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1191071"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2666
                     /gene="metH"
                     /locus_tag="AC159U_02530"
     CDS             <1..2666
                     /gene="metH"
                     /locus_tag="AC159U_02530"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3335963.1"
                     /translation="ALSIGSEKESPFIMVGERTNVTGSPRFAKLIKEDDFDTALSVAR
                     QQVENGANIIDINFDEGLLDSEKCMERFLNLISSEPDIAKVPIMIDSSKFSVIEAGLR
                     CVQGKCIVNSISLKEGEDIFIEQAHKVKRYGASMVVMAFDEKGQAANKEDKIRICERA
                     YKILTEKVGIEPEDIIFDPNILTVGTGIEEHNNYAVDFIEAVREIKKRCPGALTSGGV
                     SNVSFSFRGNNIVREAMHSAFLYHAIEAGLDMGIVNAGMLAVYDDVEKELLDKVENVL
                     LNKHPEATDELIDFAEQYKGVKSTEKKEDKKWRNQDVNKRIQYALVNGITDFIEEDTE
                     EVRASLNKPLDVIEGPLMDGMKVVGKLFGDGKMFLPQVVKSARVMKKAVAYLEPFMEE
                     EKKKNKDTKGQGKFVIATVKGDVHDIGKNIVSVVLACNNYEVKDLGVMVSCDDILKTA
                     KELNADFIGMSGLITPSLDEMMYNASEMERQGFDTPLLIGGATTSNAHTAIKIAPNYS
                     GVVSHVQDASLVVNVCNNLLNPNYSKKYIDEIKAEQEKLRKLHLDKTNLKKYLSIKEV
                     REKGLKTNWTKMNIPKPEFTGINVFENIKMDEIVDYIDWSPLFWVWELKGSFPKILKH
                     KKYGDHAEKLYDEAMSLLKEIIKDNRFNPRAVIGLFPANSIKDDIVLYSNEDRNESIY
                     TLHFLRQQEYRGEKAQYLCLSDFIAPADTGIKDYIGAFCVTAGEEVDEFASYFKNKND
                     DYNSIMVQALGDRIAEALAEMIHKKVRDYWGYGKDEKLTSDELIKEKYIGIRPAPGYP
                     ACPDHTEKRTIWNLLKVEDNIGVKLTENCAMYPPSSVSGLYFSHPKSKYFNIGKINND
                     QIEDYALRKGMTIKEVEKWLHPNLGYDPTDN"
     gene            2675..3007
                     /locus_tag="AC159U_02535"
     CDS             2675..3007
                     /locus_tag="AC159U_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR3335964.1"
                     /translation="MAYSEILAQRLTKILETKKGISNRKMFGGLCYMLNGNMLCGIVD
                     DKLMARVGPEQYDNALKHEYCTEMDFTGKPLKGMVYVLPEGIESKRSLKKWIDMCIAF
                     ASILPKKK"
     gene            complement(3009..3203)
                     /locus_tag="AC159U_02540"
     CDS             complement(3009..3203)
                     /locus_tag="AC159U_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335965.1"
                     /translation="MVMKEDIKKLCVTVYGEACPDLCPLEKDECDECSICSDPQNLEY
                     LISEDELPQKWKDFIRSCTE"
     gene            complement(3204..4640)
                     /locus_tag="AC159U_02545"
     CDS             complement(3204..4640)
                     /locus_tag="AC159U_02545"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HIR3335966.1"
                     /translation="MKTPEEFVQSLRELKPVVYLKGKRIKSVADEPKLFPGVNAISLT
                     YEYSHNPDYEDIMLATSHITGNKVSRLLHINTNTDDLLKKLEMTTRLLCRTTGCAQRY
                     LCHDALNALYENTHLVDLKTGSDYYSKFLEYLEYLQDEDLTTCVAMTDAKGDRTKRPH
                     EQKNPDLYLRIVKRTKKGITVRGIKAIVTGAPYTHEIIVLPCRTMTEKDSDYSVSFAI
                     PIDTKGVEMVTREAGRPDQSSAELSNKYGQATAQILFDDVFVPWNRVFLAGEWQFAGL
                     LTESFATHHRVSCIGARAGLGDMLIGASTTMAEANGLNPNKVGHIRQSITELIKYVES
                     FYACGVTSAVFGKETPAGNFSPDPVYANIGKLLQGTHIYDMYKVAHEVSGGILVASPY
                     PEDMEIPEVGEKLSSYLTANKDIPSDKRLKMARLLEDITASYQGGWYSIIGIHGGGSP
                     EAQRIEMMRKYDFEERKSLVAKITGLDG"
     gene            complement(4680..4973)
                     /locus_tag="AC159U_02550"
     CDS             complement(4680..4973)
                     /locus_tag="AC159U_02550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3335967.1"
                     /translation="MSVVLIAEVKIKPGTKEELVSYMREILPDTRAFEGNEGIDVCFD
                     EEDSDLMVLVEKWSTPDDYHKYHEWRVSSGTLDKLRSFLAGKPSRRFLDIADC"
     gene            complement(4986..5420)
                     /locus_tag="AC159U_02555"
     CDS             complement(4986..5420)
                     /locus_tag="AC159U_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016961335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3335968.1"
                     /translation="MKLTPFHIAVQVRDIQESREFYGQILGLEEGRSDTNWVDYNMYG
                     HQFVCHLNPDLGKDGKVTLHYNPVDKHSVPVPHAGVVLEMSDWQKLADKLTGKNMEFV
                     IKPYIRFKGKTGEQATMFFLDPSGNALEFKSFKNINENLFKK"
     gene            5525..5974
                     /locus_tag="AC159U_02560"
     CDS             5525..5974
                     /locus_tag="AC159U_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HIR3335969.1"
                     /translation="MIKKSLNVYLAGEIHSDWRERIIDGTKAKGLNVTFTSPVTDHES
                     SDMCGVNILGDEEKPFWRDRKGAGLNEIRRKTMINDADLVVVRFGPKYKQWNAAFDAG
                     YANALNKSIITLHDEEHDHALKEVDAAANASARTPEQVVEILSYVLS"
     gene            6078..6800
                     /locus_tag="AC159U_02565"
     CDS             6078..6800
                     /locus_tag="AC159U_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3335970.1"
                     /translation="MNLSNIKAIFFDAADTLFFIKDGLGKTYAAPARKYGIDPDPQEL
                     RNAFSKHFPSAPPLAFGGISNSERKSLEKEWWYQVVTKVYNDVGMFDEFDDYFDDLFE
                     VFRTTAWKIFPETNNVLTEIKNLGYAIIVVSNFDSRVYDVCGSLGIIDHFDDFIISSE
                     AGYAKPDPEIYKLALKRNNLVPEECVFIGDNYLNDYVAPTKLGLKAILLNRENENQEH
                     EVYKISNLNELLSILNNNGEQS"
     gene            6797..7231
                     /locus_tag="AC159U_02570"
     CDS             6797..7231
                     /locus_tag="AC159U_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3335971.1"
                     /translation="MTVTLLALLVAFMWGINPIMEKLSLVKASPLSVMTVRFIITSIV
                     LIGLASYHGKFQEINNLDTKTYIYIFIPAILAGVGLYVYFIALGMGESSKVVPIVATF
                     PLFTAIYAFLILKESFTTPKLIGTFLIVAGLVILNWNSVAKS"
     gene            complement(7234..7422)
                     /locus_tag="AC159U_02575"
     CDS             complement(7234..7422)
                     /locus_tag="AC159U_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HIR3335972.1"
                     /translation="MSKTIYKVVELTTVTDEEIEKVINEWVNQDWVLDTIQFAMRDAS
                     KRPAMAFILFTKNDDLKH"
     gene            complement(7428..8402)
                     /gene="hemB"
                     /locus_tag="AC159U_02580"
     CDS             complement(7428..8402)
                     /gene="hemB"
                     /locus_tag="AC159U_02580"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3335973.1"
                     /translation="MFFPITRLRRLRLNQNIRDLVKETEISLNDLIYPMFIVAGNNKS
                     QEIESMPDIYRLSVDNALKEIEEITNYGIKAILLFGIPDKKDDIATGAYSKKGIIQTA
                     VKAIKKEFPQLVVITDLCLCEYTDHGHCGVIHDGYVDNDETLELLSLTALSHAESGAD
                     IIAPSDMMDGRVAKIREVLDDNEFHNKIIMSYSAKYCSAFYGPFRDAAQSAPQYGDRK
                     SYQMDPPNVREAIREIEQDIEEGADIVMVKPALSYLDVISAVREEFNHPIAAYNVSGE
                     YSMVKAASKMGWIDEDKVVNEILISIKRAGADLIITYFAKQIAKSLNK"
BASE COUNT         2824 a         1462 c         1585 g         2706 t
ORIGIN      
        1 aagctctgag tataggatct gaaaaagaat ctccctttat aatggttggt gaaagaacca
       61 atgtgactgg ctcaccaaga tttgcaaaac taataaaaga agatgatttc gatactgcac
      121 ttagcgttgc tcgacagcag gtggaaaacg gagcaaatat aatagacata aattttgatg
      181 aaggtctttt agatagtgaa aaatgtatgg aacgtttctt aaacctcatt tcttcagaac
      241 cggatatcgc aaaagttcca ataatgatag acagctccaa attttctgtt attgaagcag
      301 gtctcaggtg tgttcagggt aaatgcatag taaactccat aagtcttaaa gagggtgaag
      361 atatatttat agaacaggcc cataaggtta aacgctatgg ggcatcaatg gtagttatgg
      421 cctttgatga aaaaggtcag gcagcaaaca aagaagataa aataagaatt tgtgaaagag
      481 catataaaat acttactgaa aaagtcggga ttgaaccaga agacattatt tttgatccaa
      541 atattctcac agtaggtacc ggtattgaag agcacaacaa ctatgctgtt gattttatag
      601 aagctgtaag agagataaag aagagatgcc cgggggctct tacaagcggt ggagtaagta
      661 acgtatcgtt ttcatttaga ggtaataaca tagtaagaga agccatgcac agtgcgtttc
      721 tctatcatgc aatagaagca ggccttgata tgggaatagt caatgccggt atgctcgcag
      781 tatatgacga cgtagaaaaa gaactcttag ataaagtaga aaatgttctt cttaataaac
      841 accctgaggc tacagatgag cttatagatt ttgctgagca gtataaagga gtaaaaagta
      901 ctgagaagaa agaagataag aaatggcgaa atcaagatgt aaacaagaga atacaatatg
      961 cacttgtaaa tggtatcacc gattttatag aggaagatac agaggaagtg cgagcatcat
     1021 taaacaagcc attagatgtt atagaaggtc cacttatgga cggcatgaag gtagtcggta
     1081 aattattcgg tgatggaaag atgtttttgc cgcaggtagt aaaaagcgct cgcgtgatga
     1141 aaaaggcagt agcatatctt gaaccattta tggaagaaga aaagaagaag aataaagata
     1201 caaaaggaca aggtaaattc gtgatagcta cggttaaagg agatgtgcat gatattggaa
     1261 agaatatcgt ttctgtagta cttgcatgta acaactacga agtaaaagat cttggggtaa
     1321 tggtttcatg tgatgatata ttaaagacag caaaggaatt aaatgcagat tttattggta
     1381 tgagcggact cattacgcca tccttagatg agatgatgta taacgcttca gagatggaaa
     1441 ggcagggctt tgatactccg cttcttatag gtggggcaac aaccagtaat gctcacaccg
     1501 caataaaaat agcccctaat tatagtggtg tcgtatctca cgtgcaggat gcatctcttg
     1561 tagtaaacgt ttgcaataat ttactgaatc ccaattactc caaaaagtat atagatgaga
     1621 ttaaagcgga acaggagaaa ctgagaaaat tacatttaga caaaacaaat ttaaaaaaat
     1681 atttgtctat taaagaagta agagaaaaag gtttaaaaac taattggact aaaatgaaca
     1741 ttcctaaacc tgaatttaca gggattaatg tctttgagaa tataaaaatg gatgaaatag
     1801 tagattacat cgattggtct ccgctgtttt gggtttggga acttaaggga agctttccaa
     1861 agatattgaa gcataaaaaa tatggtgatc atgctgaaaa gttatatgat gaagccatga
     1921 gtctgcttaa ggaaattata aaagataaca gatttaatcc aagagctgtc attggactct
     1981 ttcctgctaa ttccataaag gatgatatag tgctttattc gaatgaagat aggaatgaga
     2041 gtatttatac attacatttt ttaaggcagc aagagtaccg tggagaaaaa gctcagtatc
     2101 tttgcctatc agattttatt gctccagcag atacggggat taaagattat attggagcat
     2161 tttgtgttac tgctggtgaa gaggtagatg aatttgcatc atattttaaa aataaaaatg
     2221 atgattataa ctctataatg gtacaggctc ttggagacag aattgcagaa gcacttgcag
     2281 aaatgattca taaaaaagta agagactatt ggggctacgg aaaagacgag aaattaacaa
     2341 gcgacgagtt gataaaagaa aaatatattg gaataagacc agcgccaggt tatccggcat
     2401 gccccgacca tacagagaaa agaacaatct ggaacctgct taaggttgaa gataatatcg
     2461 gtgttaaact cactgaaaac tgtgcaatgt atcctccaag ctcagtatca ggtctatact
     2521 tctctcatcc aaaatcaaaa tacttcaata ttggcaagat taataatgat caaatagaag
     2581 attatgcatt aagaaaagga atgaccatta aagaggtcga aaagtggctt catcctaatt
     2641 tgggttatga tccaacagat aattaaataa ttttatggca tatagtgaaa tcctagcaca
     2701 aagactcact aaaatattag aaactaaaaa aggaatttca aacagaaaaa tgtttggtgg
     2761 cctatgctat atgctaaatg gcaatatgtt atgtggtata gttgatgaca aacttatggc
     2821 cagggtaggg cctgaacaat atgataatgc tttaaagcat gaatattgca cagagatgga
     2881 ttttaccgga aagcctttaa agggaatggt ttatgtatta ccagaaggga ttgaatctaa
     2941 aagatcacta aagaaatgga tagatatgtg tatagcattt gcaagtatat tgcctaagaa
     3001 aaagtaattc attcggtaca gcttctgata aaatctttcc acttttgtgg tagttcatct
     3061 tctgaaatta ggtattcaag gttttgtgga tcagaacaaa tgctacactc atcacattca
     3121 tctttttcga gtggacacaa atcggggcag gcttcaccat agacagttac acataatttc
     3181 tttatatctt ctttcattac caattaacca tcaagtcccg taatttttgc aacaagtgat
     3241 tttctttctt caaaatcata ttttctcatc atttcaattc tctgtgcttc gggtgatcca
     3301 ccaccgtgta ttccaattat agaataccat ccgccttgat atgaggccgt aatgtcctca
     3361 agtagtcttg ccatcttaag gcgtttatct gatggaatgt ctttgtttgc agtgagatag
     3421 gatgataatt tctctcctac ttctggtatt tccatatcct caggatatgg agacgcaacg
     3481 agtatcccac ctgatacctc gtgggctact ttatacatat cataaatgtg ggtgccctga
     3541 aggagttttc ctatattggc atataccggg tcgggactga aatttccagc aggggtttcc
     3601 ttcccaaata ctgccgatgt gacgccgcag gcatagaatg attcaacata tttaattagt
     3661 tctgtaatgc tttgtcttat atgtcctact ttattaggat ttaatccatt agcctcagcc
     3721 atggttgtag aagctcctat aagcatatca ccaagaccag ctctagcccc tatacagctt
     3781 accctgtgat gagttgcaaa ggattctgta aggagtccag caaactgcca ttctcctgca
     3841 agaaatactc tgttccacgg aacaaacaca tcatcaaaaa gtatttgtgc agttgcctga
     3901 ccgtatttat tagataactc ggcagaactt tgatcgggtc tccctgcttc tctagttacc
     3961 atttctactc cctttgtatc aatgggtatg gcgaatgaaa cagaataatc agaatctttc
     4021 tctgtcattg tcctgcatgg aagcacaatt atttcatgtg tataaggagc gccggttacg
     4081 atagccttga ttcctcttac agtaatacct ttttttgttc tctttactat tcttaagtat
     4141 aaatcagggt ttttctgttc atgtggtctt ttggttctgt cacctttagc atcagtcatg
     4201 gcaacacatg tggttaaatc ttcatcctgt aaatactcaa gatattctaa aaacttagaa
     4261 taataatcag aacctgtttt taaatctaca agatgcgtgt tctcatatag agcatttaag
     4321 gcatcatgac agagatatct ttgagcacaa cctgttgtcc tgcaaagaag tcttgttgtc
     4381 atttcaagtt tttttaacag atcatccgta ttagtattta tatgtagcag ccggctaact
     4441 ttatttccgg taatatgcga tgttgcaagc ataatatcct catagtctgg gttatgagaa
     4501 tattcgtatg ttagagaaat agcattaaca ccaggaaaaa gttttggctc atcggcaaca
     4561 ctttttattc tttttccttt taaatagaca acaggtttta gttctctaag gctttgtaca
     4621 aactcttcgg gtgttttcat aacatatagc tataatgttt tatgaaagac agtttaatat
     4681 cagcaatcag caatatccaa aaatcttctg cttggctttc ctgcaaggaa tgacctaagt
     4741 ttgtctaaag ttccagaaga gactctccac tcgtgatatt tatgataatc atcaggggta
     4801 ctccattttt caacaagcac cattaaatca gaatcctctt catcaaaaca aacatcgatc
     4861 ccttcattac cctcaaatgc tcttgtatca ggtaagattt ctctcatata agatactagt
     4921 tcttcttttg ttcctggttt tattttaact tcagctatta atactacact catgattaat
     4981 tctccttatt ttttgaataa attttcattt atatttttaa aagacttaaa ttcaagtgca
     5041 ttgccactag gatctaagaa aaacatagtt gcttgctctc ctgtctttcc cttaaatctt
     5101 atatatggtt ttatcacaaa ttccatattt tttccagtca gtttatctgc aagcttttgc
     5161 cagtcactca tttctaaaac taccccagca tgaggcacag ggacagaatg tttatcaaca
     5221 gggttgtaat gtagagttac tttgccatct ttacctaaat caggatttag atgacacaca
     5281 aactgatgac catacatatt atagtcaacc cagtttgtat cacttctacc ttcctctagg
     5341 ccaagtattt gcccatagaa ctctcttgat tcttgtatat ctcttacttg tacagcaata
     5401 tgaaaagggg ttaatttcat gatttatata ggttataata tatagttaaa gtattttatt
     5461 acttgaatta aagtgttttc taggtacata ttatatcagt aattctttat atggagtaat
     5521 aattatgatc aaaaaatcat taaatgtgta tcttgcaggt gaaattcact cagactggcg
     5581 agaaagaatt atagatggca caaaagcaaa gggtttaaac gttacattta cctctcctgt
     5641 gacagaccat gaatccagcg atatgtgcgg agtaaatata cttggagatg aagaaaagcc
     5701 tttctggcga gacagaaaag gcgcagggct taatgagatc agaagaaaga ccatgattaa
     5761 cgacgccgat cttgtagtag taagatttgg tcctaaatac aaacagtgga atgctgcatt
     5821 tgatgcgggc tatgcaaatg cactaaataa atcaataatc acactccatg acgaagaaca
     5881 cgaccatgct ttaaaagaag tagatgccgc tgctaatgct tcagcaagaa cccctgagca
     5941 ggttgtagag atattaagtt atgtactttc gtaaagttta aaatcttttt atcaaaccac
     6001 cctattattc aaaatttagg ctataactat ataaaactgg tatagtagag ttctattttt
     6061 attaaaggat taaagttatg aacttatcaa acataaaagc tatattcttc gatgctgctg
     6121 acactttgtt ttttataaaa gatggactgg gtaagacgta tgctgcgcct gcaaggaagt
     6181 atgggataga tcctgatcct caagaattaa gaaatgcatt ctctaaacat tttccatccg
     6241 ctccgcctct tgcatttggt ggtatttcaa attctgagag aaaatcacta gaaaaagaat
     6301 ggtggtatca ggttgtaaca aaagtataca atgatgttgg aatgttcgat gaatttgatg
     6361 attattttga cgatttattc gaagtattta gaactaccgc atggaaaatc tttccggaaa
     6421 caaataatgt gctaactgaa ataaaaaatt tgggatatgc cataatagtc gtatccaatt
     6481 ttgattcgag agtttatgat gtttgtggaa gtcttggaat tatagaccat tttgatgact
     6541 ttatcatttc gagtgaggca ggatatgcaa aacccgatcc tgagatatat aaattagcct
     6601 taaaaagaaa caaccttgtt cctgaagaat gcgtatttat aggtgataac tatctaaatg
     6661 attatgtagc accaaccaaa cttgggttaa aagcaatact acttaataga gaaaatgaga
     6721 accaggagca tgaagtatac aaaatatcaa acctaaatga attactctca attttaaata
     6781 ataatggaga acaatcatga cagtaactct tttagcatta cttgttgcct tcatgtgggg
     6841 cataaatcct ataatggaaa aattaagcct tgtgaaggcc tctcctctta gtgttatgac
     6901 cgtacgattc attattactt caatcgtact tataggtctt gcttcatacc atgggaagtt
     6961 tcaggaaata aataatctgg atactaaaac ttatatatac atttttattc cagcaatact
     7021 ggctggggta gggctatatg tttatttcat tgctcttgga atgggagaat catccaaagt
     7081 cgtacctata gttgctacat ttccactttt tactgcaata tatgcattcc tgattttaaa
     7141 agagagtttt acaacaccta aattaattgg aacatttctt attgttgcag gtcttgtaat
     7201 actaaactgg aatagtgtgg ctaaaagcta aaattagtgt ttgagatcat catttttagt
     7261 gaaaagtata aacgccatag cgggtctttt tgaggcgtct ctcatagcaa actgtattgt
     7321 atcaagcacc cagtcctgat ttacccactc atttatcact ttttcaatct cttcatcggt
     7381 gaccgtggtt agttctacta ctttataaat tgtcttactc atgaattcta cttgtttaag
     7441 gactttgcaa tttgttttgc aaaatatgtg ataattaaat cagctcctgc tcttttaatt
     7501 gaaataagta tttcatttac taccttgtct tcatctatcc atcccatctt tgaagctgcc
     7561 ttcaccattg aatattcacc gctaacatta tacgcggcaa ttggatgatt aaattcttct
     7621 cttacagcgc ttattacatc caaataagaa agcgcaggct ttaccatcac tatatccgca
     7681 ccttcttcaa tgtcctgctc aatttccctg attgcttctc ttacattagg tgggtccatt
     7741 tgataggact ttctatctcc atactgaggt gcggactgtg cagcatctct aaaaggacca
     7801 tagaaagctg agcaatattt agccgaataa gacatgatga ttttattgtg aaattcgtta
     7861 tcatccagta cttcgcgtat tttagccact cttccatcca tcatatccga tggcgctata
     7921 atatcagctc cggattctgc atgcgaaaga gcagttaatg ataataactc aagcgtctca
     7981 tcattatcaa cataaccatc gtgaataaca ccgcagtggc cgtgatcggt atattcacaa
     8041 aggcacagat cagtaataac aactaactgt ggaaattcct tttttatggc cttaactgct
     8101 gtttgtataa tacctttttt gctataggcc cctgttgcaa tatcatcctt tttgtcggga
     8161 ataccaaaaa gaagaatagc cttaatacca taattagtta tttcttcaat ctcttttaaa
     8221 gcattgtcaa ctgagagcct gtaaatatca ggcatggatt ctatctcctg agatttattg
     8281 ttacctgcta caataaacat cggatatatt agatcattta aagaaatctc tgtttctttt
     8341 acaagatcac gaatgttttg gttaagcctc agtcttctta gtcgtgttat tgggaaaaac
     8401 atagtattta taaataattt aatcaaaaaa aagggtgtta taaaaatata acaccctttc
     8461 gtttaatttt taattaagaa ttaataaact ttctgaaaat attagtccca aggttgggta
     8521 ttattttcag gagttgctga attcttaagc catttttctg atttataata agcaggt
//
LOCUS       DBNGSV010000071         4373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1329455, whole genome shotgun sequence.
ACCESSION   DBNGSV010000071 DBNGSV010000000
VERSION     DBNGSV010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1329455"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..844
                     /gene="hisA"
                     /locus_tag="AC159U_02585"
     CDS             119..844
                     /gene="hisA"
                     /locus_tag="AC159U_02585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF010112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3335974.1"
                     /translation="MQIIPAIDLKDGNCVRLLKGEEGSETIFSYSPVDQAKKWESCGA
                     QTLHLVDLDGAFTGSPKNREVIKDIVNAIDCDVQLGGGIRELSTIDDYIKSGITRIIL
                     GTAAFKDPELLKHACNKYPGRIAVGLDTKGGKIAIKGWKETIEDSTEQVLKNLENIGV
                     SMIINTNVDRDGTLEGIDTDSVKEFLDSSPVPVIASGGISTDTDLKNLSNLKSNNLYG
                     AILGKSIYQGTIDLQKAIRRYHR"
     gene            844..1608
                     /gene="hisF"
                     /locus_tag="AC159U_02590"
     CDS             844..1608
                     /gene="hisF"
                     /locus_tag="AC159U_02590"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3335975.1"
                     /translation="MLSKRIIPCLDVKDGRVVKGVNFVNLRDAGDPVEIAKQYSKEGA
                     DEITFLDITASHEGRNIMIDVVRQTASEVFVPLTVGGGVRTLDDVRNLLLAGADKVAI
                     NTAAVKDPEFVRQAAEKFGSQCIVVAIDARSNGQGSWEVYTHGGRNPTGIDAIEWAKK
                     MEDYGAGEILLTSMDKDGTKEGYDLELTKTISENLIIPIIASGGAGNLQHLCDGVKKG
                     KADAVLVASIFHYGEYKISEAKDFMASDGVVVRNVN"
     gene            1610..2656
                     /locus_tag="AC159U_02595"
     CDS             1610..2656
                     /locus_tag="AC159U_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR3335976.1"
                     /translation="MNIRKRTEEIERQTLSSHATLSDESKGRQKAEKPCNIRTCFQRD
                     RDRIIHSKSFRRLKHKTQVFLAPTSDHYRTRLTHVIEVSQIARTIARSLRLNEDLTEA
                     IALGHDLGHTPFGHAGEQVLNEIHPGGFKHVIHSLRVVDVLEKDGKGLNLSYEVRDGI
                     AKHSKGMGSIDNPKYRPETMEGQVVRVSDLVAYANHDLDDALRSGIIKLGDVPNQALK
                     VLGRTISKRVNSMVTDIITQTQNHSDGNVHIGEEVEDAIVNLRSYLFENVYMNETVKN
                     NFVKAGKIIRELYEYFCANPEKLWELKEKKYVYDKDHNQIVCDFIAGMTDSFAISLYE
                     KLFLPKRWSVEFSK"
     gene            2666..3487
                     /locus_tag="AC159U_02600"
     CDS             2666..3487
                     /locus_tag="AC159U_02600"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR3335977.1"
                     /translation="MKKVTLGTVPFLNSKPLIYPLEQNLIEHNIEVSYFIPSELSCKL
                     KSREVDIALMPVAELFKNPELMIFPGISISSLGPVESVVLLSKKSLDEIGSVALDKRS
                     RSSSALIKIILEKFYNICPQYHYRDIDEGFLADVDSGMLIGNAGLHAKFYPQQGFTVY
                     DLGQLWTEHTGLPFVYAVFAYHKDNQEKISSVSKSILESKASGQKYIEEIIKDQAPKL
                     GLEPQVCFNYLTKSIRFDLGKKEIEGMKLFGEYLQEYDIDIQITKNSKLFESLNI"
     gene            3518..4351
                     /locus_tag="AC159U_02605"
     CDS             3518..4351
                     /locus_tag="AC159U_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis family protein"
                     /protein_id="HIR3335978.1"
                     /translation="MSKLKMRLGHSPDADDAFMFYAMAKGKVDSPDVDFEHVIEDIQS
                     LNQRALNKELEVTAMSVHAYTHVQDNYRILSCGASMGEGYGPIVVAKEKLDSLQGKTI
                     AVPGKLTTAFLLLNIYAEGFIPVEMPFDKVMDAVVNGETDAALVIHEGQLTYKDFDLV
                     MLFDLGILWEKDTSLPMPLGVNTISRDIDEPLAKEILRLHKDSIVYGLEHKDEALEYA
                     MEYGRGMELSVGEEFVLMYVNDLTVDMGDKGRQALELLFSKAREKGIITKDVKLDILE
                     G"
BASE COUNT         1434 a          772 c         1070 g         1097 t
ORIGIN      
        1 aatattgcag aggttggtaa gtttttcaaa cgaaagggta agtgggtaac tgtctgggag
       61 cttacagaag aaggaataat cattacggca aataagataa aggaactact tgaagcaaat
      121 gcaaataata ccggcgatag accttaaaga tggaaactgt gtaaggcttt taaaaggaga
      181 agaaggctca gaaacaatat tctcatacag ccccgttgat caggcaaaaa aatgggagtc
      241 ctgcggagcg caaactcttc accttgttga cctcgacggc gcttttacag gatcacccaa
      301 gaacagagaa gtaattaaag atatagtaaa cgcgattgat tgcgatgttc agctgggtgg
      361 cggaattaga gaactatcga ccatagacga ttacataaaa tcaggaatta cgagaattat
      421 attaggcact gctgctttca aagaccctga gcttctaaag catgcctgca ataaatatcc
      481 cggccgcatt gctgttgggc ttgatacaaa gggcggcaag attgcaatta aggggtggaa
      541 ggagaccata gaggactcaa cggagcaggt gttaaaaaac ctggaaaata tcggtgtttc
      601 aatgattatt aataccaatg tagacagaga cggcactctt gagggtatag atacagattc
      661 agtaaaggaa tttttagatt cctccccggt accggtgata gcttcgggtg ggatatctac
      721 agatactgat ctaaaaaatc taagcaactt aaaatcgaat aacttatatg gagccatact
      781 gggaaaatcc atataccagg gcacaatcga cctccaaaaa gctatccgga ggtatcatag
      841 ataatgctct ctaaaagaat tattccttgc ctcgatgtaa aagacggaag ggtcgtaaag
      901 ggtgtaaatt ttgtaaacct gcgtgatgca ggagatcctg tggagatagc caaacagtat
      961 tccaaggaag gcgcagacga gattactttc cttgatatta cagcatctca tgagggaagg
     1021 aatatcatga ttgatgtagt aaggcagacg gcaagcgagg tgtttgtgcc tcttacagtg
     1081 ggaggtggag taaggactct tgacgatgta agaaatttgc tcctagcggg tgcagataag
     1141 gtggcaataa atacagctgc cgttaaggac cccgaatttg taagacaggc tgccgaaaaa
     1201 tttggaagtc agtgcattgt cgtggctatt gatgcgagat caaacgggca gggcagctgg
     1261 gaagtctata cgcatggagg cagaaacccc acgggaattg atgccataga gtgggctaaa
     1321 aagatggaag attatggtgc tggtgaaatt cttcttacaa gcatggacaa ggacggcacg
     1381 aaagaggggt atgatctgga gcttacaaaa acgatctctg agaaccttat tattcccata
     1441 atagcatcgg gcggagccgg caaccttcag cacctttgtg acggggtaaa aaaaggaaag
     1501 gccgatgccg tgcttgtggc ttcaatcttt cattatggcg aatacaaaat atccgaggca
     1561 aaagatttca tggcctcaga cggagtagta gtaagaaacg taaactaaat tgaatataag
     1621 aaaaagaacc gaagaaatag aaaggcagac gctgtcttct catgcaaccc tgagtgatga
     1681 gtctaaaggc aggcagaagg ctgaaaagcc ctgcaatata cgtacctgtt ttcaaagaga
     1741 ccgggacagg atcattcatt caaaatcatt caggcgtctt aagcacaaga ctcaggtctt
     1801 tttggcgcca acaagtgatc attacagaac aagactaaca catgtgattg aagtatccca
     1861 gattgcaaga accattgcca ggtcgctcag attgaatgaa gaccttaccg aggcaatcgc
     1921 tcttggtcat gaccttgggc acacgccgtt tggtcatgcc ggtgagcagg tgctaaatga
     1981 gattcacccc ggaggtttta agcatgtaat acacagcctt cgtgtggtag atgttctgga
     2041 aaaggacggc aaggggctta atttgtccta tgaagtaaga gacggcattg caaagcattc
     2101 aaagggcatg ggttcgatag ataatccaaa gtacaggccg gaaaccatgg aggggcaggt
     2161 tgtaagggta tctgatcttg tggcttatgc caatcacgac ctcgatgatg cgctgaggtc
     2221 gggaattatt aagcttggtg atgttccaaa tcaggctcta aaggtgcttg gcagaacaat
     2281 ttccaaaagg gttaattcca tggttaccga tattattact cagacacaga atcattcaga
     2341 cggtaatgtg cacataggtg aagaagtaga agatgctatc gtaaatttga gaagttattt
     2401 atttgaaaat gtatatatga atgaaacggt taaaaacaat tttgtaaaag ccggcaaaat
     2461 aataagggag ctctacgaat atttttgtgc caatccagaa aaactctggg agctaaaaga
     2521 gaagaaatat gtgtatgata aagatcacaa ccagatagta tgcgatttta tagcaggcat
     2581 gacagattct tttgcaatat cactctatga aaaactgttt cttccaaaaa gatggagtgt
     2641 ggaattttct aagtaaaata tgactatgaa aaaagtaact cttggcacgg ttcctttcct
     2701 aaactccaaa ccgcttatat atcctctgga acaaaatctg attgagcaca atattgaagt
     2761 ctcttacttt attccatcgg agctttcttg caagcttaag agcagggaag tggatatagc
     2821 gcttatgccc gtggccgagc tttttaaaaa tcccgagctt atgatatttc ccggaatttc
     2881 aatttcatct ctgggcccgg tggaaagcgt agtgctgctg tcaaaaaaga gtttggatga
     2941 aattggatcg gttgcgcttg ataaaagatc tagaagctct tcagctctta taaaaattat
     3001 actggagaaa ttttataata tttgccctca gtaccattac agagacatag acgagggatt
     3061 ccttgcagat gtagactcgg ggatgctaat aggaaatgcg gggcttcatg caaaatttta
     3121 cccgcagcag gggtttacgg tttatgacct ggggcagctt tggaccgaac acacaggtct
     3181 tccttttgtg tatgctgtat ttgcctatca taaagataat caagaaaaga ttagtagtgt
     3241 tagcaaaagt attttagaat caaaagcaag cgggcaaaaa tacatagagg aaataataaa
     3301 agaccaggcg ccgaagcttg gacttgagcc gcaggtttgt tttaactatt taaccaaaag
     3361 cataagattt gaccttggca aaaaagagat tgagggtatg aaattgtttg gtgaatattt
     3421 gcaggaatat gatatagata tacaaatcac taaaaattct aagctttttg aaagcttaaa
     3481 catataaaca taattagaca aaaaggagat tgtaccattg agcaagctaa agatgagact
     3541 aggacacagc cccgacgcag acgacgcatt tatgttttat gcaatggcaa aaggaaaagt
     3601 agattcaccg gacgtagatt ttgagcatgt gattgaagat attcagtcgc ttaaccagag
     3661 ggcactgaac aaggagttag aggtaacagc aatgtcggtt catgcctaca cgcatgtgca
     3721 ggacaactac cgcatactct catgcggagc aagcatgggc gaggggtatg ggcctatagt
     3781 agttgcaaag gaaaaattag acagcctaca aggaaagaca atcgccgtcc cgggaaagct
     3841 tactacagca tttcttttac ttaatatata tgcagaggga tttatccctg tggaaatgcc
     3901 ttttgataag gtgatggatg cggttgtaaa cggagaaact gacgctgcgc ttgtaataca
     3961 tgagggtcag ctcacataca aggattttga tctggttatg ctttttgatc ttggaattct
     4021 ctgggaaaaa gatacatcac ttccaatgcc tcttggagta aacactatta gcagagatat
     4081 agacgagcct ctagccaaag agattctcag gcttcataag gacagcatcg tctacggcct
     4141 ggagcataaa gacgaggcgc ttgagtatgc aatggaatac ggaagaggaa tggagctatc
     4201 tgtaggagaa gaatttgtgc tcatgtatgt aaacgatctt acagtagaca tgggagacaa
     4261 aggcaggcaa gcactcgaac ttcttttttc aaaagcaaga gagaagggta ttattactaa
     4321 agatgtgaag ctagatattc ttgaggggtg agggtagggg cagaatccat tag
//
LOCUS       DBNGSV010000072         5655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1883561, whole genome shotgun sequence.
ACCESSION   DBNGSV010000072 DBNGSV010000000
VERSION     DBNGSV010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1883561"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /gene="ppsA"
                     /locus_tag="AC159U_02610"
     CDS             <1..2172
                     /gene="ppsA"
                     /locus_tag="AC159U_02610"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR3335979.1"
                     /translation="KSLSNKAKKVRGLILGSAIPDDLWSEIKQAYDRLCEQYGKNTDV
                     AVRSSATAEDLPTASFAGQQETYLNIRGYSSLKEACSKCFASLFTDRAISYRIDHKFD
                     HFKIALSIGIQKMVRSDLATSGVMFTLDTDSGFRDVVFITACYGLGENIVQGAVNPDE
                     YYVFKPTFKRGFDSIIRKNLGEKKIKMIYGTGQLKALTRNVEVPLSDRKKFCVNDEDI
                     LKLAGYATVIEDHYSKRAGKPQHKDIEWAKDGLTGELFVVQSRPETVQSQKSQEVLQT
                     YYLDTKSSLLAKGKSVGEKIASGKTRIVESTEQLHAFKNGEVLVSNTTTPDWEPVMKT
                     ASAIVTNRGGRTCHAAIVSRELGIPAVVGTLDGTEKIKAGKQVTVSCAEGDEGFVYDG
                     ILPFHIDSVELKDIKRPKTKIMINLANPDIAFSTSMIPNDGVGLARMEFIINSYIKIH
                     PMALVHYKKVSDSSVRKQIDSLTYGYEKKEDYFVEKLAYGVGTIAAAFYPKPVIVRMS
                     DFKTNEYANLIGGQYFEPSEENPMIGFRGASRYYDNRYREGFALECAAMKKVREVMGL
                     DNLIIMIPFCRRLEEAEKVLNEMEKNGLKRGDKGLQVYVMCEIPNNILLADEFSALFD
                     GFSIGSNDLTQLTLGVDRDSEIIAHVFDERDSGVMKFISMAIKAAKKNKSHIGICGQA
                     PSDYPEFARFLVNEGIESISLNPDTVIKTTLDILELEENLR"
     gene            2260..4974
                     /locus_tag="AC159U_02615"
     CDS             2260..4974
                     /locus_tag="AC159U_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3335980.1"
                     /translation="MENNTAGKSPNTTSWHTQDAHTSIALLNTDLQNGLDSDEARKRL
                     SKYGENLIRSVSKQTWHMVLARQFKDVLILILTIAAVVSVVVGEISDAITITIIVALN
                     GILGFVQEWKAEKAIEALQRMMEPVCNVVRGGAINTIEAKYLVPGDIVQLETGDRVPA
                     DLRIIEELNLKTDESILTGESVSVSKTSKPVLEKAPLSERGSITWMGTTVTNGRARCV
                     VIATGMQTEFGRIAKLTETIKREKTPLQNKLGILGKQLGAIAIIISVLVAASGLILGK
                     ELFNMFFTGVALAVAIVPEGLPAVVTVTLALGIRSMVRRRALLRRLQAGETLGSATVI
                     CTDKTGTLTQNQMTVQKIWLLSGEINVGGIGYKPEGGFQKDGSVIEPGKLYDLMALLE
                     AGLKCNHSGIARDGDYWYAIGEPTESALVVAANKAGLHPDNNREFISEFSFNSTRKRM
                     TVVEQLPDSLVAYVKGAPEVIIKRSSKVLDKDTERILSRNDLININNAYKKYAEMGLR
                     TLAIARRKIPKESELDEQNVECELTLIGIVGIIDPPRAEVPNAIKLAHSAGIKNIMIT
                     GDAPATALAIAKMIGMKVNRAINGEELSTMDDQALQNVLSREVLFARTTPEDKLRIVN
                     MLQDMGHVVGMTGDGVNDAPALKKADIGIAMGLRGTEVAKGASDIILTDDNFASIIGA
                     VEEGRRQYDSIQKFVRYLLSSNTGEVVAIFINIIIGGPLILLPVQILWMNLITDGVTA
                     VALGLEPAEKGIMQRPPRNTKEPILNRPGIIKILVLGSYMGIVTLLLFYYYLYSKDQL
                     LVIKAQTVAFTGLIILEKINVLNFRALRAPLFNVGYLTNPWILAALFGMIGLQVCAVY
                     LPFLQDALHTVPLSLYDWGLILIFAVPVFIVFEIYKMIKWRLHSKDVM"
     gene            5024..>5655
                     /locus_tag="AC159U_02620"
     CDS             5024..>5655
                     /locus_tag="AC159U_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3335981.1"
                     /translation="MTGYEIVKLVNYEKLVGAHTVDRISKKAEKLKDFDVAHINSTYY
                     GGGVAEILSSLTLLMNSVGIKTEWRVIQGSPDFFSITKKVHNALQGEKLNLSRRKTDI
                     YESVIYENSLRNHLNHDLVIIHDPQPLGMINHYKKHCPWVWRCHLDLSCPNKRLWNYM
                     VPYIEKYDAVILTLKEYKKTLKTPQVFFMPAIDPFSIKNKKLSNKEIDER"
BASE COUNT         1813 a         1038 c         1309 g         1495 t
ORIGIN      
        1 aaaagcctgt ccaataaagc taagaaagta aggggtttaa tacttggctc agcaattcct
       61 gatgatcttt ggagtgagat taagcaagca tatgacaggc tttgtgagca atatggcaag
      121 aatacagatg ttgccgtaag aagctctgca accgccgagg acctgccgac tgcatctttt
      181 gcaggacagc aggaaacata tctaaacata agaggataca gctcactcaa agaggcttgc
      241 agcaagtgct ttgcatcgct ttttacagac agggcaatat cataccgtat tgaccataaa
      301 tttgatcatt ttaaaattgc tctctcaatc ggcatacaga agatggtgcg ttccgatctg
      361 gctacaagcg gggtaatgtt taccctcgac acagatagtg gttttcgtga tgtagtgttt
      421 attaccgcct gctatgggct tggtgaaaac atagtgcagg gtgcagtgaa ccctgatgag
      481 tactatgtat ttaaacctac attcaaaagg ggctttgatt ctatcatccg caagaatctt
      541 ggtgaaaaga aaattaaaat gatttacggc accggccagt tgaaagctct tacaagaaac
      601 gtcgaggtgc cgctttcaga ccggaaaaag ttctgtgtga atgatgagga tattttaaaa
      661 cttgctggat atgccactgt tattgaagat cactactcaa agagagcggg caaacctcag
      721 cataaagata tagagtgggc aaaggacggc ctcacaggag aacttttcgt tgtgcagtcc
      781 cgaccagaaa cagtacaatc ccaaaaatca caggaagttc tgcagacata ttatctggac
      841 acaaagagca gtttattggc caagggaaaa agtgtgggag agaaaatagc ctcgggtaaa
      901 acacggatag tagaaagtac tgaacaacta cacgcattta aaaatgggga agtgcttgtt
      961 tcaaacacta ccactcctga ttgggagcct gtcatgaaaa cagcttctgc aattgtcaca
     1021 aacagagggg ggcgaacctg ccatgctgca atagtaagcc gtgaactcgg aatcccggct
     1081 gtagtcggca cattggacgg caccgagaaa ataaaagccg gaaaacaggt tacagttagc
     1141 tgcgcagaag gtgacgaagg atttgtatat gatggtattt tgccgtttca tatagacagc
     1201 gttgaattaa aggatataaa aagaccaaaa accaagataa tgattaatct tgcaaacccc
     1261 gacatagctt tttctacctc tatgatacca aacgatggag taggtcttgc acgaatggag
     1321 ttcattataa acagctatat aaaaattcat cccatggctc ttgttcatta taaaaaagtg
     1381 tctgattcat ctgtaagaaa acagattgat agtctcactt atggttatga aaaaaaagag
     1441 gattattttg ttgagaagtt agcatatggt gtgggcacta tagcggctgc tttttatcca
     1501 aaacctgtaa tagttcgcat gagtgatttt aagacaaacg aatatgcaaa tcttatcgga
     1561 gggcagtatt tcgagccttc tgaagaaaat ccgatgatag gattcagggg agcatcgcgc
     1621 tactatgaca acagataccg tgagggattt gcactcgaat gtgcagcaat gaaaaaagtg
     1681 agagaggtta tggggctcga taatcttatt atcatgatcc cgttttgcag gagactcgaa
     1741 gaggcagaga aagtactgaa tgaaatggag aaaaacggac tcaagcgagg ggacaagggt
     1801 ctgcaggttt atgtgatgtg tgagattcca aacaatatac tcttagcaga cgagtttagc
     1861 gcgctgtttg acggcttttc gattgggtct aatgacctta cgcagcttac ccttggagtc
     1921 gatcgtgact cagagataat cgcacacgtt tttgatgaaa gggattcggg agttatgaaa
     1981 tttatatcta tggcaataaa agcggcaaag aaaaataaaa gccatattgg tatttgcggg
     2041 caggccccga gtgattatcc cgagtttgca aggtttttag tgaatgaggg aatagaatcg
     2101 atctcactta atcctgatac agtaattaaa actacactcg atattttaga actggaagag
     2161 aatttgaggt aataaatgcg gctaattttt gagtatgtta tatatattat ggaaatttat
     2221 taatagataa gaccaactgg ccaccatatt aattacctga tggaaaataa tactgctggt
     2281 aaaagcccta acaccacctc ctggcatacc caggatgcac acacatcaat cgccttatta
     2341 aatacagatt tgcaaaatgg cctggactct gatgaggccc gtaaaagact atccaaatac
     2401 ggagagaacc tgatacgctc tgtgagcaag cagacctggc acatggtttt ggcaaggcag
     2461 ttcaaagacg ttcttattct aattcttacc attgcggctg tcgtttcagt agttgttggt
     2521 gaaataagcg acgcaattac tattactata attgtggccc tcaatggcat tttgggattt
     2581 gttcaggaat ggaaagcaga aaaagccata gaagcacttc aaagaatgat ggaacctgtc
     2641 tgcaatgttg taaggggcgg tgctataaac accattgagg caaaatattt ggtgcctgga
     2701 gacatagtgc agttagagac aggagacagg gttcctgctg atctaagaat aatagaagag
     2761 cttaatctta agaccgatga atccattctc actggtgaat cagtgtctgt cagcaaaact
     2821 agcaaacccg ttttagaaaa ggctccgctt tcggagcgag gatcaattac atggatgggt
     2881 actacagtta caaacggccg ggccaggtgt gtggttatag caacaggtat gcagaccgag
     2941 tttggtcgta tcgcaaagct tacagagacg attaaacgag aaaagacccc tctgcagaac
     3001 aagcttggca tactgggaaa acagcttgga gcaatagcca ttatcatttc tgttttggtg
     3061 gcagcttcag gacttatttt gggaaaagag ttgttcaata tgttttttac aggtgttgca
     3121 cttgcggtag caatagtacc tgaaggactt cctgcggttg tcaccgtaac actagctctg
     3181 ggaatacgct ctatggtcag acgcagagca cttctgcgcc gactgcaggc tggtgaaacg
     3241 cttggttctg ctactgttat ctgtacggac aagacaggca ctttaaccca aaaccaaatg
     3301 actgtacaaa agatatggct tctaagcggt gagattaatg tgggagggat aggctataaa
     3361 ccagaggggg gttttcaaaa agatgggtca gtgattgagc caggcaagct ttatgatctc
     3421 atggcattac tggaggctgg ccttaaatgt aaccattccg gtatagcccg cgacggagac
     3481 tattggtatg cgattggaga gcctacagaa tcagcccttg ttgttgcagc aaataaagct
     3541 ggattgcatc ctgataacaa cagggaattt ataagtgagt tctcatttaa ctctaccaga
     3601 aagagaatga cagtggtcga acaattacct gactccttgg ttgcttacgt taaaggagct
     3661 cctgaagtaa taatcaaaag aagctctaag gtgcttgaca aagatacaga aaggatattg
     3721 agcagaaacg atttgataaa tatcaacaat gcttataaaa aatatgcaga gatgggtctg
     3781 cgcacccttg caatagccag acgtaaaatt ccaaaggaat ctgaactaga cgagcaaaac
     3841 gtagagtgtg aacttacact aatcggaata gtcggaataa tagacccacc gcgagcagag
     3901 gtgccaaatg caattaagct tgctcatagt gcgggaataa aaaatatcat gataacagga
     3961 gacgcccctg ccactgcact tgctatcgca aaaatgatcg gcatgaaagt aaacagagcc
     4021 ataaacggcg aggagctttc aacaatggat gaccaggctc tacagaatgt tttatctcgg
     4081 gaggttttat tcgctcgtac gactcccgaa gataagctta gaatcgttaa tatgctccag
     4141 gatatggggc atgttgtagg catgacagga gacggagtaa acgatgcacc tgctctaaag
     4201 aaggcggata taggaatcgc catgggacta aggggtactg aggtggcaaa gggcgcatca
     4261 gatattatat taactgatga taactttgca tctatcatag gagcggtcga agagggcaga
     4321 cgacagtatg atagcatcca gaaatttgta agatatctgc tttcatcaaa cacgggagag
     4381 gttgttgcaa tatttataaa tattattata ggcgggccac ttattttact acccgttcag
     4441 attttatgga tgaatttaat aaccgacggg gtaaccgcgg ttgcccttgg tttggagcct
     4501 gcagagaaag gcataatgca gcgtccaccc agaaatacca aagaaccaat tctaaaccga
     4561 cccggaatca ttaaaattct ggtgttggga tcgtatatgg gaatcgtgac attacttcta
     4621 ttctattatt atctatacag taaagatcag ctattagtca taaaagcgca aaccgtagcc
     4681 tttactggtc ttattatcct ggaaaaaatt aatgtgctga actttcgcgc tctaagagcc
     4741 ccgcttttca atgtaggtta tttaactaat ccgtggatac ttgctgcgct ctttggaatg
     4801 ataggtctac aggtgtgtgc tgtgtatctg cctttcttac aggatgcact tcatacagtt
     4861 ccacttagcc tttatgattg gggcctgata ttgatatttg ctgttcctgt ttttattgtt
     4921 tttgagatat ataaaatgat taagtggagg cttcatagta aagatgtaat gtaaacacag
     4981 cggtaacagc gtttataata tatttgtata gaggtaaaaa attatgacag gatatgaaat
     5041 agttaaacta gtgaattatg aaaaactggt aggtgctcat acggttgacc gcataagcaa
     5101 aaaggcagaa aagttaaaag acttcgacgt tgctcatata aattcaactt attatggtgg
     5161 tggtgttgcc gaaatactgt cgtctctcac actgctcatg aacagtgtcg ggattaaaac
     5221 agaatggaga gttatacagg ggtcacctga ttttttcagt attacaaaaa aagttcataa
     5281 tgcacttcaa ggcgaaaagc taaatctgag cagacgcaaa acagatatat atgaaagcgt
     5341 tatatatgaa aactctctca gaaatcactt aaatcacgac ctggtaatca tccatgatcc
     5401 ccaacctttg gggatgataa atcactataa aaaacactgt ccctgggtct ggcgctgcca
     5461 tcttgatctt agctgtccta ataaaaggtt gtggaactat atggttccat atatagaaaa
     5521 gtatgatgcg gtaattctca cgttaaaaga atataaaaag acacttaaaa ctccacaggt
     5581 tttctttatg cctgccatcg atccttttag tattaaaaac aagaaattga gcaacaaaga
     5641 gattgatgaa cgaat
//
LOCUS       DBNGSV010000073         1489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_429335, whole genome shotgun sequence.
ACCESSION   DBNGSV010000073 DBNGSV010000000
VERSION     DBNGSV010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_429335"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      178..252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            443..991
                     /locus_tag="AC159U_02625"
     CDS             443..991
                     /locus_tag="AC159U_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335982.1"
                     /translation="MIRLVLLLAILGMLSGCAAFRNDLQSYQEDLPKYKYKILPEQPY
                     DNQGPAYAKYVDHIKQEVVVVPLRKAATFEEGEGDRIKVRFDGGVVLTFEWVNSKRKY
                     YLVDEPITLNGVTYKCDWDSVTYAKWTDPETGKNFRKKLAPNLKYYLGAYKLKQGELI
                     EKNETNDQKVAFQHYTVNEEMN"
     gene            complement(1060..1224)
                     /locus_tag="AC159U_02630"
     CDS             complement(1060..1224)
                     /locus_tag="AC159U_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335983.1"
                     /translation="MVLTTCNYTQSSTVPSIVEHYTRTSAIAADLTSIAFYGAGTTRT
                     KKGHLTGALN"
BASE COUNT          454 a          289 c          326 g          420 t
ORIGIN      
        1 gtattataca ttgtattggc ttttggcatg cgcgactcat gatgactggg caaatgtaaa
       61 aaccccgaca aaagcgggta agaaccagat aaggtctacc ttaaaaaatc agctaataat
      121 taatcaaact tgacactcct tccatccttt gcgatattat ccggcaacat gaaaactctc
      181 cgataagagc aaactttgag aaatcaaggg acgcagagtc acagacccat aatgggtggc
      241 tggtccgccg aagagagatg gcattctttg tcttagctga tgcgctattg ctgtatagtt
      301 gtatgcattt acacgcgtgt ccactcttga cttatccgcc ctctttccct ctctcttctt
      361 tgactcatat gtcggttgat tgttcagacc aacttcaaaa atagcgtctg ctatttaaat
      421 aatcgcatgg gggaaatgaa aaatgattag acttgtactg ttacttgcaa tcttggggat
      481 gttatctggc tgcgctgcct ttaggaatga ccttcagtca taccaggagg atctgccgaa
      541 atataaatac aaaatccttc cagagcaacc gtacgacaat cagggtccgg cgtacgcaaa
      601 atatgttgac cacataaaac aggaagttgt tgtggttcca ctcagaaaag cagctacatt
      661 tgaagagggc gagggggaca ggataaaagt tcgttttgat ggcggagtag ttcttacctt
      721 tgagtgggtt aatagtaaaa gaaaatacta tctggtagat gagcccataa cgctaaatgg
      781 ggttacttac aaatgtgatt gggatagcgt cacatatgca aaatggactg atcccgaaac
      841 cggaaagaat tttcgcaaaa aactcgcccc taaccttaag tactatcttg gagcctacaa
      901 gttgaaacag ggtgaattaa ttgaaaagaa cgaaacaaat gaccaaaaag ttgcatttca
      961 acactataca gtaaatgaag agatgaatta gcactacaaa cgtaacagct ttcaggtagt
     1021 acgttgactg tatgtaaata ccttctggta tttttctttc taatttaggg caccagttag
     1081 gtgccctttt tttgtcctgg tcgttcctgc cccgtaaaaa gctatagagg taaggtcagc
     1141 cgctatagca gaagttctcg tgtaatgttc tacaattgat ggtactgtgg aagactgggt
     1201 gtagttacaa gtggtcaaga ccacttcagt acagcttcat cctaaacttg cattgcttag
     1261 ctatgccgtc taaactattc gaacatatgt ttcttaaaaa aactggaggt taattctatg
     1321 atgaacaatg tgaagttaat tggaacactg gttctcgcaa taatggtatt ccaaacattt
     1381 ggtgcgccta tagcctcggc ggcgttgatt gagtccgaag agattgtata tgagattcaa
     1441 atagatgatg ctcggcaaaa attgtcgaca gctattgagc gttcagatg
//
LOCUS       DBNGSV010000074         8951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_221316, whole genome shotgun sequence.
ACCESSION   DBNGSV010000074 DBNGSV010000000
VERSION     DBNGSV010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_221316"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC159U_02635"
     CDS             <1..194
                     /locus_tag="AC159U_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR3335984.1"
                     /translation="ILTPREEEIIRLRFGIDKESTYTLDEIGKRFDLTRERIRQIEKA
                     ALEKLQRSDAGNVLASFLR"
     gene            complement(217..1302)
                     /locus_tag="AC159U_02640"
     CDS             complement(217..1302)
                     /locus_tag="AC159U_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3335985.1"
                     /translation="MTRNEVVSWFFIGIFLYLMYLFVQVILPFIIPLFWAAILVLILY
                     PAHEKLTKLLRNRVGLSSIIMTLLTVWLIVIPLFLLVSSLALEMVDIYSSAKSKGEID
                     KLASYVSKITESDSLEKILPTSIINGLENKLDLGEVNVKSILVKSSKSISSYLINLFK
                     GFASNITSMLFSFGIMIFSLFFFFRDGRQMFEKLKYLIPMSEEQKNRTFKVFHNTIDG
                     VVVGSLATAAVQGLLVLIIFLILGISYPVLAGSISFVLSILPLVGATFVWLPVTIYLL
                     ATGVFLKGIVLLVFGIVVISMSDNIIRPIIIGGKVKLPTFFLLLSIMGGLDYFGFSGI
                     ILGPVLLAVFMSFIEIYKQEYRDKKVV"
     gene            1383..2861
                     /locus_tag="AC159U_02645"
     CDS             1383..2861
                     /locus_tag="AC159U_02645"
                     /inference="COORDINATES: protein motif:HMM:NF018471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HIR3335986.1"
                     /translation="MQFKNSNYSNATWIFIRCIGAIYLIAFLSFFFQYDGLIGQNGIL
                     PFARYLDSLQTNLGSRAYWLVPTISWLNQTDTFLYWQLIISLVFSVALIAGVLPIISS
                     LILFISYLSIVNIGQSFMTFQWDILLLEAGFLAILISPVKLLSRLRDNKTPHILLIIL
                     LKLLLFKLMFSSGIGKLLSGDETWKNLTALNFHYFTQPLPNQIAWYAHQLPEWIHKTS
                     VAVMLFIEIIVPFFFFAPARPRYLAGFLTIGLQIIIMATGNYTFFNLLSIVLCLFLFD
                     DDFFKIFYRKSGLSFKDESITPSTTLSKIKLGSLSLIFFLIVSLSVIQFSRRYLGVRN
                     LPEFVNKAVLYSSSYHIVNNYGLFTVMTTKRNEIIIEGSNDGINWKAYEFKYKPGDLK
                     GGLHFVAPHQPRLDWQMWFAAFGNYRRNHWFVNLMYRLQEGKPEVLGLIKGNPFPDKP
                     PKFLRAQLYDYKFTNWEERKSSGDIWKRTYLKYYMPVLRSSR"
     gene            complement(3241..6894)
                     /locus_tag="AC159U_02650"
     CDS             complement(3241..6894)
                     /locus_tag="AC159U_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335987.1"
                     /translation="MVSDKSKFLSFIAIMLFSCIVMFGLSSTSHAGVATDLQVFKTGV
                     QPDCDFTSLGGLFGITDELQNCTKDEDVFAGEEFFVLVQVRNFDTEDADDVIVIDDFP
                     FGWTLLEVSGTQFSSDPAEPDLPITCAPTDSSVCEDTGGDDPEASCIRCQIGDLNNAV
                     IPGIIPEINSFLLKFQVNSNHVVGFSNGVTAVLNTAYTGLEGGAILDQYTDSTIVQDD
                     ADLEITKVCNHDLPLDSGDTVQCTAIIKNNGTSWSRDVILRDTITFAPGSSIAPFSIG
                     PFSENQDVNGFNRFCTFVQNNPNTSPFVFECELGVLPNEDQVTVSYTFTANQGTNVTD
                     VVQAICHTQTTEGTPDPDCSNNMATDEVLFENSADLSIQKLSSAAGGPVGPGDSILYT
                     LTISNNGPGSANNVRVVDILPDHVEINGLILAPLGSVCFQEIINKKIVCDIGGVLNPF
                     APGDGPAVITIPVEVQAGASGSLQNIAMVSSDTADPITSNNTSVTNDAVQAVVADLEI
                     SKEVFPDPTVAGEVQTAIITVTNNGPDDVTSVQIRDIFPQALIPIEPLPSGCTLAGSG
                     DIECNLTGIPAGFSRIIEILFLIPSDYLINDPNNDGFASCSDGVSIQMINNMPDEDVD
                     PITFNNTAFASTFCVDESDLKITKVCKPDEPLFAGDTGFCDIFVDNLGPSDARDVLMT
                     DEILSEGLFDITDVTVIQSCTDCTVTCDPTSASMVMEETVVCDLGDLTAIQSDNRTAI
                     RISVQANEDVDINNVATVNSLTPDPYLPNNMAEGSINVDGIADMRVQKSCPDTVVAGG
                     PAFDYLIDIGNWGPSQAVNVVVEDVVPAGLSINSVVGSGGAQCETGVPGVATLPTVCN
                     YDSVNDNDAFTQTMTINVSVPPDLTGILHNDVMVSSDTFDDFNINNIDTCDTTITQSA
                     DLVVTKVVTTPASGPVSAGDPLGWTVTITNNGPSSATGISLTDILPPEVTFDSANILL
                     GTGACQFFPPPPDAIVECNFTDVVDPGESVIVAILSTVNNDVPNGTDIENCVSATSDA
                     FDPDEVNNTDICANIDSAVSADLWMEKDSNFPTGNPSGTVQYFCTVHNDEGCSELPHD
                     ATGSNVCGTGGPSNAENVVIRDYLPLNPKKVIFQFASQGCFYNQSGHYVQCEADLIQH
                     GDAVTYEIQVDFKGSVKEDIINRCEIISSDTPDPDGSNNEHHLRTVVQGGTGDPGGPG
                     GGRGRGGNNGRTK"
     gene            complement(7065..7376)
                     /locus_tag="AC159U_02655"
     CDS             complement(7065..7376)
                     /locus_tag="AC159U_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335988.1"
                     /translation="MKQDINNELDSELSSNVAYEILDYLAHNPNAEGSIESIAEWWLL
                     EQRINKEMEGIMKAVQQLVENHLLVERRSEDMKVYYSVNREKLNQILELLNKKMTANG
                     H"
     gene            complement(7959..>8951)
                     /locus_tag="AC159U_02660"
     CDS             complement(7959..>8951)
                     /locus_tag="AC159U_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3335989.1"
                     /translation="EKHEELKKEVKTKYRPDSAMIGESHELQEVLKLIEKVRDSNITV
                     SITGETGTGKEIVARAIHHLSPRSGMPFITFDCGAVPPELVENELFGHFKGAYTSATS
                     SETGLINAAEGGTLFLDEIDALNTSSQVKLLRLLQEKEYKPLGSAKIQKADIRFIAAS
                     NKNLKKAVKEGRFRSDLYYRLNIIPLKLPSLNKRTGDISLLAEHFLVKYINKFQKILD
                     GFSDAALQKLQFYDWPGNVRELENTIERAVVLCRSKMIEPEDISLEEIEDIDIDRSFK
                     DLKSDVIERFETSYIKSLLIKHDGNVTKAATAAQKNRRAFWQLMKKYNIDVSSH"
BASE COUNT         2613 a         1911 c         1641 g         2786 t
ORIGIN      
        1 ctatacttac tccaagagaa gaagagataa taagactgcg ttttggtata gacaaggaaa
       61 gcacatatac tcttgatgaa ataggtaaaa ggtttgacct tacaagggaa aggatcaggc
      121 agattgaaaa agccgcacta gaaaaacttc agagatcaga tgctggcaat gtgcttgcaa
      181 gctttttaag atagttaaaa ttagaagtga tcaggatcag accactttct tgtccctata
      241 ttcctgctta tagatctcta taaaactcat aaatactgca agaagcaccg gccccaggat
      301 tattcccgaa aatccgaagt aatcgagccc ccccataata cttaataaaa ggaaaaatgt
      361 aggtagtttc acttttcctc ctattatgat aggccttata atattgtcag acatacttat
      421 cacaactatc ccaaatacaa gaagaactat tccctttaga aaaactcctg tagctaaaag
      481 atatatagta acaggaagcc agacaaatgt agcacccaca agtggtaata tcgataaaac
      541 aaaactgata gatcctgcaa gcaccggata agaaattcct aatattagaa atattattag
      601 tacaagcaga ccttgtacag cagcagtagc aaggcttcca acaacaactc catctatagt
      661 gttatgaaaa accttaaacg ttctgttttt ctgttcttca ctcatgggta ttaggtattt
      721 tagtttttca aacatctgcc gtccgtccct gaaaaagaag aatagtgaga aaatcataat
      781 accaaaacta aaaagcatac ttgttatatt gcttgcaaac cctttaaaga gattaataag
      841 atagctgcta atagatttag aagatttcac taaaatactt tttacgttta cctctcccag
      901 gtctaacttg ttttcaagtc catttatgat tgaagttgga agtatttttt caagagaatc
      961 agactctgta atttttgaaa catatgatgc cagtttatca atctctccct tactttttgc
     1021 tgaggaataa atatcaacca tttcaagcgc aaggctgctt acaagtaaaa atagagggat
     1081 aacaataagc caaaccgtaa gaagtgtcat gataatggac gacaacccaa ccctgtttct
     1141 taaaagtttt gtgagttttt catgcgcagg ataaagaata agtactagaa ttgcagccca
     1201 aaagagtggg ataataaatg gcaatataac ctgaacaaaa aggtacatca ggtataaaaa
     1261 tattccaata aaaaaccagg agactacttc atttcttgtc attccatgtt gccatgtatt
     1321 tatattatta tgtaattaac ataatttatt ttaataaata aagtataata gatcatttag
     1381 aaatgcaatt taaaaatagt aattattcta atgcaacatg gatctttata agatgcatag
     1441 gggctattta tcttatagca ttcctttcat tcttttttca atatgatggc cttataggac
     1501 aaaacggaat tttaccgttt gcaaggtatt tagattcact gcagacaaac cttggtagtc
     1561 gtgcatattg gcttgtacct acaataagct ggcttaacca gacagataca tttctatatt
     1621 ggcagcttat aataagcctc gttttctctg ttgccctaat cgcaggagtc ttacccatca
     1681 tttcatcact aatactgttt atatcgtatc tatccatagt aaacatcggc cagtctttta
     1741 tgaccttcca gtgggatata cttttgctcg aagctggatt tttagcaata ctaataagcc
     1801 ccgtaaaact actatcaagg ctaagagata ataaaacacc gcacatacta ttaattattc
     1861 ttttaaaact tctccttttc aaactcatgt tttcatcagg aataggcaag ctgctaagcg
     1921 gtgatgagac atggaaaaat ctaactgcac taaacttcca ttatttcact cagcccctac
     1981 caaaccagat tgcctggtat gcacaccagc tgccggagtg gatacataaa acttcagtag
     2041 cggtaatgct atttatagaa atcatagttc cttttttctt ctttgcacca gcaagaccaa
     2101 gatacttagc cggcttttta acaataggcc ttcaaataat catcatggcc actggaaact
     2161 atacgttttt taacctgctc tcaatcgtgc tttgtctgtt tttattcgat gatgactttt
     2221 ttaaaatatt ttatcgtaaa agcggcctat cctttaagga tgagtctata accccgagca
     2281 caacactttc aaaaatcaaa ctaggatctc tctcactaat attcttctta attgtaagtc
     2341 tctcagtaat tcagttctca agaaggtatc ttggggtaag aaacttgcct gaatttgtaa
     2401 ataaagctgt actctattcg tcttcctacc acattgtaaa taactatggc ctttttacgg
     2461 ttatgaccac taaaaggaat gaaattataa tagaaggaag taatgacggc ataaactgga
     2521 aggcgtatga atttaaatac aagcctggag atttaaaagg aggtcttcat tttgtagccc
     2581 ctcatcagcc ccggcttgac tggcagatgt ggtttgcagc atttgggaat tacagacgta
     2641 atcactggtt tgtaaatcta atgtacagac tacaggaagg aaaaccagaa gttttaggac
     2701 tgataaaggg aaatccattt ccggacaagc ctcccaaatt tttaagagca caactctatg
     2761 attataaatt tacaaattgg gaggaaagaa aatcatcagg cgatatttgg aaaagaactt
     2821 atttaaaata ttacatgcct gttttaagaa gcagccggtg atttttgtat tttggcttta
     2881 actaatccaa cgttattaat cattcacagg ttctctatcc tgatagaccg taattttttc
     2941 tattcgaaaa aacccgtttt ctcatcctag gaaaagtaca tatggactaa ccttgtcaga
     3001 aatattcttt ccgcatttta aatacgagat tgtcacgtcg ctccgctcct cacaatgaca
     3061 gttgtgggat gtcgtttgct aaagtaaaga aagcccaccc tataattctc tgacacccta
     3121 ataaaaaaag gagcagtgac caaatagtca ctgctcccta tgtttacttc ttattgcttt
     3181 tcattcacta ccgggaagta accttccccg caggtttata tcttattaaa atcgaggctt
     3241 ctatttagtt ctaccgttgt taccacctct gcctctacca cctcctgggc cacccggatc
     3301 accggttccg ccctggacaa cagttcttag atgatgctcg ttattggagc catccggatc
     3361 aggtgtgtct gaagaaatta tctcacatct gttaataata tcttctttta cactaccttt
     3421 gaagtcaacc tgaatctcat aggtcacagc gtctccatgc tgaatcagat cagcttcaca
     3481 ctgtacatag tgtccggatt gattgtagaa acagccttgt gatgcgaact ggaagattac
     3541 cttcttcgga ttcagtggca gataatctct tattaccacg ttctcagcat ttgaaggtcc
     3601 gcctgttcca cacacattac tgcccgtagc atcatgtggc agctccgaac agccctcatc
     3661 attatgtacc gtacagaagt actgtaccgt gcccgatggg tttcctgttg gaaagttgct
     3721 gtccttttcc atccacaggt ctgcagaaac tgcagaatct atgtttgcac agatgtccgt
     3781 gttgtttacc tcatcgggat caaatgcatc cgatgtagca cttacacagt tttcaatatc
     3841 agtaccatta ggcacgtcat tgttaaccgt gctcagtatg gctactatta cagactctcc
     3901 cggatccaca acgtctgtga agttacattc aacaatcgca tctggtggtg gtgggaagaa
     3961 ctgacatgca cccgttccaa gaagtatatt tgcactatca aacgttactt ccggtggaag
     4021 tatgtctgtc agtgatatac cagtggctga tgatggcccg ttgtttgtaa ttgtaactgt
     4081 ccatccaagt gggtcgcctg cgcttacagg accgcttgct ggcgttgtta caacttttgt
     4141 aactaccaga tcggcgctct gtgttattgt tgtatcacat gtatcgatgt tattgatatt
     4201 aaagtcatcg aatgtgtcag aagaaaccat aacatcatta tgaagtattc cagttagatc
     4261 aggcggaaca cttacattga tagtcatagt ctgagtaaat gcgtcgttat cgtttacact
     4321 gtcatagtta cacactgtag gaagtgttgc aactccgggt acacctgtct cacactgtgc
     4381 tcctccggaa cctactacag aattaatcga aagccctgcc ggcaccacat cttcaacaac
     4441 aacattaact gcctgtgaag ggccccagtt tcctatgtct atcagataat cgaaagcggg
     4501 tcctcctgcc actactgtgt caggacacga cttctggact ctcatatctg caataccgtc
     4561 tacatttatc gatccctctg ccatattgtt tggcaggtat ggatcaggcg tgaggctgtt
     4621 tacagtggca acgttgttaa tatcaacgtc ttcattagcc tgtactgaaa tccttattgc
     4681 cgttctgtta tcagactgta tagccgtgag gtcacccagg tcacagacaa ctgtttcttc
     4741 catcaccatg cttgcgcttg taggatcaca ggttaccgta cagtctgtgc atgactgaat
     4801 aacagttacg tctgtaatat caaacaaacc ttcactcaaa atctcatcag tcataagaac
     4861 atctcttgcg tctgatggcc ctaggttgtc tacgaatata tcacagaagc ctgtgtcacc
     4921 tgcaaatagc ggctcatctg gtttacaaac ctttgtaatc ttaaggtctg attcgtctac
     4981 gcaaaatgta gaagcaaatg cggtattatt aaaagttatg ggatctacat cttcatccgg
     5041 catattatta atcatctgta tagatacacc atccgaacat gatgcaaaac catcattgtt
     5101 tggatcattg attaaatagt cactaggaat caaaaacagg atttcaataa ttctgctaaa
     5161 acccgcaggt attcctgtta gattacattc tatatctcca gatcccgcaa gagtacaacc
     5221 agatggaagt ggttcgattg gtattagagc ttgaggaaat atatctctaa tttgaacact
     5281 agttacatca tccgggccat tattggtgac tgtaattata gcagtttgta cttcacctgc
     5341 tacagttgga tccggaaata cctcttttga aatctcgaga tcagctacaa cagcttgaac
     5401 cgcatcattt gtcacagaag tgttgttgct tgtgattgga tcggcagtat cacttgatac
     5461 cattgctatg ttttgcagtg acccgcttgc accagcctgt acctctacag gaattgtaat
     5521 aacagccgga ccatcaccgg gagcaaaagg attcaaaacc ccgcctatat cacaaactat
     5581 tttcttattt attatctcct gaaaacaaac tgatcccaga ggtgctagaa taagaccgtt
     5641 tatttcaaca tggtcaggta atatatctac tacccttaca ttatttgccg aacctggccc
     5701 gttgttgcta atagttagag tatagagaat tgaatctcct ggaccaacag gacctcctgc
     5761 agcagatgaa agcttttgaa ttgaaagatc ggcagagttt tcaaacaaca cttcgtcggt
     5821 tgccatgttg tttgagcagt caggatcagg tgtgccttct gttgtttggg tatggcatat
     5881 tgcctgcact acatcggtta cgtttgtacc ctgatttgca gtaaatgtat aagacactgt
     5941 cacctgatct tcatttggaa gcacgccaag ttcacactca aatacgaatg ggcttgtatt
     6001 aggattattc tgaacgaatg tgcagaatct gttaaaacca tttacgtcct gattctcact
     6061 gaatggccca atgctaaatg gggctattga gctgccagga gcaaatgtga tggtatctct
     6121 cagtatcaca tcccttgacc aggaagttcc gttattctta ataatagcag tacactgtac
     6181 tgtgtctccc gaatctaatg gaagatcgtg gttacagact tttgtaattt caaggtctgc
     6241 atcgtcctga actatggtcg aatctgtgta ctggtcgaga atagctccgc cttccaaacc
     6301 agtgtatgca gtgttaagta cagcggtaac gccattacta aaaccaacta catgattact
     6361 gtttacctgg aattttagta agaacgaatt gatttctgga ataatgccgg ggattacggc
     6421 attattcaga tctcctatct gacagcgtat acagcttgct tcaggatcgt ctccaccagt
     6481 atcttcacac acagaagaat ctgtaggtgc acatgtaatt ggtaaatcgg gttctgccgg
     6541 atcactactg aactgcgtac cgcttacttc aagaagtgtc cacccaaatg gaaaatcatc
     6601 aattactatg acgtcatccg cgtcttcagt gtcaaagttt ctcacctgta ctaggacaaa
     6661 gaactcttca cctgcaaata cgtcttcgtc tttcgtacag ttctgcagct cgtctgtaat
     6721 tccaaacaaa ccgccaagtg atgtaaaatc acaatcaggc tgaaccccgg tcttgaagac
     6781 ctgtaggtcc gttgccactc cagcatgtga agtgctggat agtccgaaca taacaatgca
     6841 gctgaaaagc attattgcta tgaatgataa aaacttactt ttatcactta ccatcttttt
     6901 ttcctccttt tattaggatt tacatattta ttgctaaaac ctgaagtctc ttagattatt
     6961 ggatttaaga aaccccactt tatttccatc tctatattgc taaattttgt ttttctccct
     7021 ttacctcctt ttatccgttt ttacgtttat tttgtttgtc atagttaatg tccgtttgca
     7081 gtcatttttt tatttagcag ttcaagtatc tgatttagtt tttctcggtt tacgctgtaa
     7141 tagaccttca tgtcctccga tctccgctct actaataagt gattttcaac taactgctgc
     7201 acagctttca taatcccttc catctcttta tttattctct gctcaagaag ccaccactca
     7261 gctatggatt ctatggaacc ctctgcattt gggttatgag caagatagtc cagtatttcg
     7321 tatgccacgt ttgagcttaa ttctgaatcc agttcattat ttatgtcttg cttcacgagc
     7381 agactccttt ggtatttatt cttgcatgta taagtgcaac cacagtgcca ggttgtaact
     7441 gcctgtgatc attgaataat ataaatttga gcaggataaa tttgtgactc tggttttcag
     7501 ataatttagt tagagcatca gatttatagg ggtaaaacta gtggacattt ttgtcataaa
     7561 aaagtcacaa aaatgtcctg gttggatgta ataggggtct ggatgtacca caaatgtcat
     7621 tgcgaagctc aaacattcat acagttatca aaaatgagct gtggcaatct cgtagttaga
     7681 tcaggaacaa cgtttcaaga tagacaaagt tagagggttt tttaaaaacg agatcatcac
     7741 gtcgtacaat ccgaattaaa taactaatga agtactcctc atgatgacag ttgtggttag
     7801 tcactgccta agctcacact tcacctgtcc cgagctatgt cgaagtgcct gccgaagtat
     7861 agtgaagctg ataccttccc aacccgtcat gcttgagcgt aagcgaagca tcttatttta
     7921 gattcttcgg ctttgcctca gaatgacaag aagaaagttt aatgcgaaga aacatcaata
     7981 ttatacttct tcataagctg ccagaatgct cttctgttct tctgggccgc tgtggcagcc
     8041 ttggtgacgt ttccatcatg cttaataagg agggatttta tatagcttgt ctcaaaacgc
     8101 tcaatcacgt ctgacttaag gtccttgaat gacctgtcaa tatctatatc ctctatttct
     8161 tccaggctta tatcctcagg ctctatcatc ttacttctgc aaagaacaac cgcacgctct
     8221 atagtatttt caagttccct cacgtttccg ggccagtcat aaaactgtaa cttctgtagg
     8281 gcagcatctg agaaaccatc gagaatcttc tggaatttat ttatgtattt aacaagaaaa
     8341 tgctctgcaa ggagtgaaat gtctccggtt ctcttgttta gtgaaggaag ctttagcggt
     8401 ataatattta atctatagta taggtcagac ctaaacctgc cctcttttac ggctttttta
     8461 agatttttat tcgacgctgc tataaaacgt atatctgcct tctgaatttt tgccgacccc
     8521 aggggcttat attctttttc ctgcagaagc cgtaagagtt taacttgaga cgaggtatta
     8581 agcgcatcga tctcatcgag aaaaagtgtg ccgccttcgg ccgcatttat aagccctgtt
     8641 tctgacgagg tagcacttgt atatgcccct ttgaaatgac caaatagctc attttctaca
     8701 agctccgggg gcacagcgcc acaatcaaaa gtgataaaag gcatgcctga tctggggctt
     8761 aagtggtgaa ttgccctggc tactatctct tttcctgtgc cggtttctcc ggtaatggat
     8821 acggtaatgt tggaatccct gaccttttca atcaatttca aaacctcctg aagctcatga
     8881 ctttctccga tcattgctga atcaggcctg tatttggttt ttacctcctt ttttaattcc
     8941 tcgtgcttct c
//
LOCUS       DBNGSV010000075         8393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1814970, whole genome shotgun sequence.
ACCESSION   DBNGSV010000075 DBNGSV010000000
VERSION     DBNGSV010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1814970"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC159U_02665"
     CDS             <1..494
                     /locus_tag="AC159U_02665"
                     /inference="COORDINATES: protein motif:HMM:NF036831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3335990.1"
                     /translation="NPFEYKGTFPLPENQLDRFAIRISIGYPDYESEKEIMTNSNPYN
                     SSDQLSPVISKDDILQLQSMVNNVTIDESILEYILDIVNKTRDNSLFDLGVSPRGTIA
                     FKLAAQANAIVEGRDYCIPDDVKNMAVAVLAHRVIMKTETSKVSTEEEAIKEILEQIK
                     VPH"
     gene            complement(524..799)
                     /locus_tag="AC159U_02670"
     CDS             complement(524..799)
                     /locus_tag="AC159U_02670"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3335991.1"
                     /translation="MLLSEMDIENNLSSLDGWGQAGLEIEKQYKFKNFIESVGFVNKV
                     AILAEKADHHPDILIQYSKVKISLSTHSEGGITDKDFSLAGEIEAVS"
     gene            853..1344
                     /gene="folK"
                     /locus_tag="AC159U_02675"
     CDS             853..1344
                     /gene="folK"
                     /locus_tag="AC159U_02675"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3335992.1"
                     /translation="MNIVYLSLGSNIENRIEHCIKALDELSAYVDLEDVSSFYETEAL
                     GVTGQNNFINCAAKIKTPLMPLDLLSSLITTEKKLGRLSKSDYSPRTIDLDIIFYNDL
                     TLKSDKLVIPHKQAHLRRFVIEPLCELCPDLIHPVLNKSISNILENISSAQIVKKIGT
                     FYQ"
     gene            1596..2579
                     /locus_tag="AC159U_02680"
     CDS             1596..2579
                     /locus_tag="AC159U_02680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3335993.1"
                     /translation="MKYGIGYYSLQSPPHKPRKHAELYKEMLDEISTADQMGFDSAWL
                     TEHHFLADGYCPSLMVTAGAIAARTKNIRIGTGVLLIPLHDPVRIAEDAAVVDLISEG
                     RLILGLGLGYRKEEFDGFGRALKERKGRIEESLDIFTRAWADGKFSHNGKYYRLKDVN
                     ITPKPVQQPIPIWIGAFTEPAIRRAARIGAPLYVPAIGVIPIIKALFGMHSSMLEEYG
                     HNPDEIEKPLVREMYISDKKSDDAWEDIKEHVTYTAKGYASWGSMVDREGNLLSDPND
                     PVLYGIARDQAIIGTPEECIDQINEYKENLPVNNIICRFKFPGISHDEAMR"
     gene            complement(2618..3493)
                     /locus_tag="AC159U_02685"
     CDS             complement(2618..3493)
                     /locus_tag="AC159U_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3335994.1"
                     /translation="MSSNNWKIILLIFTIITWGYSWILMKQSLQYMEPFTFIALRHVV
                     AAIVLLPLMLFHGSFKLKNFSNPHFFLIGLIQTAAMMAFIIYGMKFVTAGKTSVIVYT
                     MPVWTSLLLHFLLKEKLNNAKWTGVGLGLIGTLCILGWDAISVQSRDIIYGEALIILA
                     AVCWAASNILNRKKLNNENPFIVNGYQLFYGAVILVILAYISEGAFKVDWNYHSVFIV
                     LFTGIVASAINFSIWFYLINSYDINITTSSSLLVPVVGIVLDWFILGTTLDIGVLAGG
                     LLIVLGLYKISTAKA"
     gene            3650..3997
                     /locus_tag="AC159U_02690"
     CDS             3650..3997
                     /locus_tag="AC159U_02690"
                     /inference="COORDINATES: protein motif:HMM:NF045455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-fold beta flower protein"
                     /protein_id="HIR3335995.1"
                     /translation="MPKYVYDDQGNPVGYINSGYIHTLHGQAVGQLNGENVHKMNGEY
                     VGKLQDGMVIDTKERHPERIGHRGDPGNPGYYGVPDNRGAVESDYPVVFTKLLDRVNY
                     VDESVNKFKKKLL"
     gene            complement(4251..4925)
                     /locus_tag="AC159U_02695"
     CDS             complement(4251..4925)
                     /locus_tag="AC159U_02695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR3335996.1"
                     /translation="MGDQKESARQIHNLIKKSCPKAKTLLELGCGTGSYLKYLSKYYE
                     TTGIDSSSVMLSIAREKLPDTELYQASMLEVKLERKFDVVICMNDTFNHLTKIADLKR
                     LFSNSFDHLIEKGILIFDINTEHKLRSLSETPPIVHQFDENYFITNVTRENKDIYEWD
                     LRIFEHISDNTYNLYEELLYERSYSVAQIKDYLGKKFKNIRVLDLSSKKTSPESQRLH
                     FTCTKK"
     gene            complement(4973..6907)
                     /locus_tag="AC159U_02700"
     CDS             complement(4973..6907)
                     /locus_tag="AC159U_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3335997.1"
                     /translation="MPLISLNEVKLSFGGPLLFDDLNLQIEHGERVALIGRNGVGKTS
                     LMKVIARELDIDDGKITYQKGLTVTHLPQEIPKQMEGKAFDIVLSGLGERAKLINDYY
                     HLSREVSSKSSPELLKRLDALQSELERTDGWKIENEVESVISHLGLEPESEFRNLSGG
                     QKRRVLLARALVINPDLLMLDEPTNHLDIDSIQWLEEFLKACKSTLFFVTHDRKFMKN
                     LATRIIELDRGSIFNWQCDYDTFLQRKQQELDSEGKHNALFDKKLAAEELWLRKGVRA
                     RRTRNEGRVRALMKMREEKRARRTEIGNAKMLTQKSEISGRQVIKATKISFSYHDQKL
                     IHDFSTLIMRGEKVGIIGPNGSGKTTLINLLLGNIKPQEGEVIVGTNLEIAYFDQLRE
                     QLDESKNVMDNVGGGKDTVTINGKDRHIIGYLQDFLFSSDRCKEKIHRLSGGERNRLV
                     LAKLFTRPSNLLVMDEPTNDLDIETLELLEDLLVEYPGTIILVSHDREFLDNVVTSTI
                     VFEGNGKLNEYPGGYYDWLYQREKLVYNSQNKKEEKADIEIIKPVKKNSPKPRKLSFK
                     ENKELEELPQIIEKLEQEQKEIFTTLSDAGYYKKDPKEIESTQARSEQIEKEISNAYE
                     RWNYLEQLREEIQREAATTN"
     gene            complement(6968..7855)
                     /locus_tag="AC159U_02705"
     CDS             complement(6968..7855)
                     /locus_tag="AC159U_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3335998.1"
                     /translation="MIKLFTVLSLLCFLYPQIVLSESVTFTFNPPDGYKVISYGKSNR
                     VYYIDGKKNKIQSNKINTETIFTKNLNGFKIIRKVILAESFENGKRLESPAGKVLIGN
                     VMTYITDNKGNLLSIEGLDSLLNNLKKTIHPDKQHLAVKVFNTEVRKKKEFDDWQNNI
                     TKFVGKSVKDGTVGYTISSLNLYGDILKYLKQMNFVDVKKDSVKINYKYTTKKPTIHN
                     WLKEINPVVVEKLNLDQIDYSSYGINGSGYEIINPKTMISKEKHSKIILEYVTTYDDG
                     SQHEIKRIQTKNTRYEIAN"
     gene            complement(7863..>8393)
                     /locus_tag="AC159U_02710"
     CDS             complement(7863..>8393)
                     /locus_tag="AC159U_02710"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR3335999.1"
                     /translation="TILRTVSPLKDVDGFHPYNVGLLVEGNPRFVSCTPAGIIRMLEH
                     YNLDIKGKEVVVVGRSNIVGKPVSMLLLHRHGTVTICHSRTKDLKEVANRADILVAAI
                     GRADFITGDMVKDGAVVIDVGINRKDDGKLTGDVDFDAVSEKASYITPVPGGVGPMTI
                     AMLLYNTYLSAKSTAE"
BASE COUNT         2609 a         1581 c         1461 g         2742 t
ORIGIN      
        1 aaaacccgtt tgagtataaa ggaacgtttc cactcccgga aaaccagctg gacagattcg
       61 cgataagaat aagcattgga tatccggatt atgagagtga aaaagaaata atgacaaatt
      121 caaatcccta caattcaagc gatcagttaa gccccgtaat ttcaaaagat gatattttac
      181 aattgcaatc aatggtaaat aacgtaacca tagacgaatc catacttgaa tacattcttg
      241 atatagtaaa caagactcgt gataattcat tgtttgacct aggagtaagc ccaagaggaa
      301 caatagcttt caagctggcg gctcaggcaa atgcaatagt tgaaggcaga gattactgca
      361 ttcccgatga cgtgaaaaac atggcggtag cagtacttgc gcaccgcgtt ataatgaaaa
      421 cggaaacctc aaaagtatct acagaagaag aggcgataaa agaaatactt gaacaaatta
      481 aagtgccgca ttaattattt agtagttcta tttctgctaa gaattaacta acagcttcta
      541 tttcacctgc aagactaaaa tctttatctg ttattcctcc ctcactatga gtagagagtg
      601 aaatcttaac ttttgaatac tgtataagaa tatcaggatg atgatctgcc ttttctgcaa
      661 gtattgccac tttatttaca aaaccaacag attcaatgaa atttttaaat ttgtactgct
      721 tttctatctc aagcccggcc tgtccccagc cgtcaagtga tgacagatta ttttcaatat
      781 ccatttctga aagaagcatt ggcccctcct taaattagga attttgtaaa tgttaattat
      841 tatacaatat agatgaacat agtttacttg agtttaggct caaatataga aaacagaata
      901 gaacactgca taaaggcttt agacgaactg tctgcatatg tagatttaga agatgtatct
      961 tccttttatg aaaccgaggc cctgggagtt acgggtcaaa ataatttcat aaactgtgca
     1021 gcaaaaataa aaactccatt gatgcccctg gatttactga gctctttaat aactacagaa
     1081 aaaaagcttg gaaggctgag taaatctgat tatagccctc gaacaattga tcttgatatt
     1141 attttttata acgatttaac tcttaagagc gataaacttg taattcccca caagcaggcg
     1201 catttaagaa ggtttgtgat agaaccttta tgtgaactgt gcccagatct gattcatccc
     1261 gtattaaaca agtcaatttc gaatattctt gaaaacataa gtagcgctca gattgtaaag
     1321 aagatcggta ctttctatca ataaacatta attaacaatc tatcaacatg ttgttaacta
     1381 tttataaata ttgatgccaa catattatta acaggcacaa atattataat gtacaatagt
     1441 tacagtatgt tatttatata ttctaaatat ttagtcaaat tatgtgaaaa gaataatgta
     1501 tttttaccaa aagttatcca cagtatattc actattgatt aaataagaat attttgatat
     1561 aaaggattaa taatactaaa aagaggtatt caataatgaa atacggaata ggatattaca
     1621 gtcttcagtc accaccgcat aaaccaagaa aacacgctga gctttataaa gagatgctgg
     1681 atgaaattag cacggcagat caaatgggtt ttgactctgc atggcttact gagcatcatt
     1741 tccttgctga tggatactgc ccatcgctta tggtaacagc aggcgccatt gccgcaagaa
     1801 ctaagaacat aagaattgga acaggtgtac ttcttattcc tcttcacgac cccgtacgta
     1861 ttgccgaaga tgctgctgta gtagatctta tttcagaagg cagactaata ctgggtcttg
     1921 gacttggata caggaaggag gaatttgatg gctttggaag ggcgctcaaa gaaagaaaag
     1981 gcaggattga agaaagcctc gatattttta ccagggcatg ggcagatgga aagttcagcc
     2041 acaatggtaa atattataga ttaaaagatg ttaatataac tccaaaacca gtacagcagc
     2101 ccatccctat ttggatcgga gcattcacag aacctgcaat aagaagggca gctagaattg
     2161 gagcgccgct gtatgtgccg gcaattggag tgattccaat aataaaagca ctatttggaa
     2221 tgcattcgtc aatgctggaa gagtatggtc ataatcctga tgaaattgaa aaaccacttg
     2281 taagagagat gtatatatct gataaaaaga gtgatgacgc ttgggaagat attaaagaac
     2341 acgttacata cacagcaaag ggatatgcct cctggggatc tatggtagac agggaaggca
     2401 acttgttaag tgatccgaat gatccggttc tatacggtat agctagggat caggctatca
     2461 taggaactcc ggaagagtgt atagatcaaa tcaatgaata taaggaaaac ctaccggtta
     2521 ataacattat ttgcaggttc aagtttcctg gaataagcca cgatgaggcc atgagatgaa
     2581 tgaagctttt tgcagataag gttttgccga aggtgtctta agcttttgcg gttgaaattt
     2641 tatacagacc cagaacaatc aacagacctc cggcaagcac acctatgtcc agtgttgttc
     2701 caagtatgaa ccagtctaaa actatgccta caacaggaac aagaagggat gatgaagtgg
     2761 taatattgat atcatagctg tttatgagat aaaaccatat ggaaaaattt attgctgaag
     2821 cgactatgcc tgtaaaaagc acaatgaata cagaatgata gttccagtct accttaaaag
     2881 ctccttctga tatatacgcc agtattacta aaataacggc accgtaaaaa agctgataac
     2941 catttacaat aaagggattt tcattgttta gtttttttct gtttaatata tttgaggcag
     3001 cccaacagac agcagcaagt attattaatg cttctccata tattatatct ctgctctgaa
     3061 ccgaaatcgc atcccagcct aaaatgcaaa gcgtaccaat aagccctaat ccaactcctg
     3121 tccatttagc attattcagc ttttctttta gtaagaaatg taacaacaga cttgtccaca
     3181 cgggcattgt atagactatc acagaggttt tacctgccgt tacaaacttc atcccgtaga
     3241 ttataaaagc catcatggca gcggtttgaa taaggccaat taaaaaaaag tgcggattgc
     3301 taaaattctt taacttaaaa gaaccatgaa aaagcataag aggtagcagt acaatagcag
     3361 ccacaacatg cctgagggct ataaaagtaa atggttccat atactgaagt gattgcttca
     3421 ttagtatcca tgagtagccc cacgtaatga ttgtaaaaat taatagaatt atcttccagt
     3481 tgtttgatga cattaaggtt ttatattaat gattaataga gaaatacaaa ctctttagat
     3541 ctaataactc cacaaattat gattttttag ttaaaatatt tacatatacc agcgcattat
     3601 ccatcagctt taatttatat gggctaaaat atatttagga gatttcaaga tgccaaaata
     3661 tgtttatgat gatcagggaa accctgtagg ttatataaat tcaggttata ttcatactct
     3721 gcatggtcag gccgtaggac agctaaatgg agaaaatgtc cacaagatga acggtgagta
     3781 tgtgggaaag cttcaggatg gcatggttat tgatactaaa gagagacatc ctgagaggat
     3841 tggccatagg ggtgatccgg gaaatccagg gtattatggt gttccggata acaggggtgc
     3901 agttgaatca gactatccgg tagtttttac caaactttta gaccgggtta attatgtaga
     3961 tgagtctgta aacaaattca agaaaaaact tctttgattt aggatacttg cagaccattt
     4021 attaatattt aataattaat tcggatatta cccaaattct gtctactcac tcaatatata
     4081 atatttcagt aagaataaaa ttaaagattg acaattaagt attgtgcttt atattattaa
     4141 taaatattaa gttaattact taattacatg ccatcctcca tcctccttta attaggaaaa
     4201 tgcgggtgta agcaatttag tttactcccg cattttatta tcaagagcaa ttacttttta
     4261 gtacatgtaa aatgcagcct ttgagattca gggcttgttt ttttgctgct taaatctaaa
     4321 actctaatat ttttaaattt tttaccaaga taatccttta tttgggcaac cgaataggac
     4381 cgctcataca acagttcttc atacaggttg tatgtattat cagatatgtg ctcaaaaatc
     4441 ctcagatccc attcatatat atctttattc tcacgggtta catttgtaat aaaatagttt
     4501 tcatcaaatt gatgaacaat tggtggagtt tctgaaagag accttagttt gtgttctgta
     4561 tttatatcaa agattaatat tcccttttct attagatgat caaatgaatt tgaaaataat
     4621 ctctttagat ctgctatttt tgttaggtgg ttgaatgtat cattcataca aataaccaca
     4681 tcaaacttcc tttccagttt aacctcgagc atgcttgcct ggtaaagctc tgtatccgga
     4741 agcttttctc ttgcaattga taacatgaca gaagaactat caataccggt agtttcataa
     4801 tatttggaca aatattttaa gtatgagccc gtgccgcagc caagttccag taaagttttt
     4861 gctttagggc aactcttctt aatcagatta tgtatctgtc tggcagattc tttctgatca
     4921 cccatgatat catcataaaa ttttccaata acatcgtaat ctgacatagt atttaattag
     4981 ttgtagctgc ctctctttgt atttcttctc ttagctgttc caggtaattc caacgttcgt
     5041 atgcatttga aatttctttt tcaatttgtt ctgaccttgc ctgagtggat tcaatttctt
     5101 ttggatcctt tttatagtaa cctgcatctg aaagggtagt aaaaatctct ttttgttcct
     5161 gctcaagctt ttctattatt tgcggtagtt cttccagttc tttattttct ttaaaactaa
     5221 gcttcctggg tttgggtgaa ttcttcttta ccggctttat tatctctata tctgcttttt
     5281 cttctttttt attctgcgaa ttataaacca gcttttctct ttgatagagc cagtcatagt
     5341 agccccccgg atattcattt aatttaccat taccttcaaa tacaattgta gaagtgacaa
     5401 cattgtctaa aaattcccta tcgtggctta ctaaaatgat agtacccgga tattcaacca
     5461 gcaggtcttc cagcagttcc aaagtttcaa tatcgaggtc atttgtaggc tcgtccatca
     5521 cgagcagatt agagggtcga gtaaataatt ttgcaagcac aagcctgttg cgctcgcccc
     5581 ccgaaagcct gtgtatcttt tccttgcacc tgtctgaaga aaaaagaaaa tcctgcaggt
     5641 atccaataat atgacggtct ttgccgttaa tcgtgacagt atcttttccc ccgccaacat
     5701 tgtccattac gtttttgctt tcatccagct gttctctcag ctgatcaaag taggcaattt
     5761 caagattggt gcctacaata acctctccct cttgtggttt aatatttcct agtaacagat
     5821 taatcaacgt agtctttcct gatccattgg gaccaataat tcctactttc tcaccacgca
     5881 taataagtgt agaaaagtca tgaattagtt tttgatcatg atagctaaaa ctaatttttg
     5941 tagccttgat tacctggcgt cctgaaatct cggatttttg agtaagcatt ttagcgttgc
     6001 caatttctgt cctgcgggca cgtttttctt ctctcatttt catgagggct ctcacacggc
     6061 cctcatttct tgtccgcctt gctcttactc cctttctgag ccataattct tcagcagcaa
     6121 gttttttatc aaaaagcgca ttatgctttc cttcagaatc caactcctgc tgctttctct
     6181 gtaaaaatgt atcgtaatca cactgccagt taaatatgct cccgcggtca agctctataa
     6241 tccgtgtagc aagatttttc ataaatttcc tgtcatgagt tacaaaaaaa agtgtgcttt
     6301 tacatgcctt taaaaattct tcaagccatt gtattgaatc aatatccaga tggtttgtgg
     6361 gctcatcaag catgagtaga tcaggattaa taactaaagc tcttgcaagc agtacccttc
     6421 ttttctgccc gccagagaga ttgcgaaatt cactttcggg ttcgagacca aggtgactaa
     6481 ttaccgattc cacctcgttt tcaatcttcc agccatctgt cctctcaagc tcggactgca
     6541 atgcatcgag ccttttaagt aattcgggac tgctcttact acttacctct cgactcagat
     6601 ggtaatagtc attaataagc tttgcccgct ctccaagtcc ggatagaaca atatcaaatg
     6661 ctttaccctc catctgtttt ggaatttcct gaggaagatg agtaacagtt aaaccttttt
     6721 gataagtgat cttaccatca tcaatatcca actcccgagc aataaccttc ataagcgagg
     6781 tcttaccaac tccgttcctt ccaataagcg caaccctctc accatgctca atctgtaagt
     6841 tcagatcatc aaaaagcaga ggcccaccaa aggaaagttt aacttcgttt agactgatta
     6901 aaggcatttt gtattagtta tatatgtaat aatttggaaa gcagtgagaa acggatacta
     6961 attattatca attggctatt tcatatctag tattttttgt ttgtattctt tttatttcgt
     7021 gttgggaacc atcatcataa gtagttacat attctaatat tattttacta tgcttttctt
     7081 tgctgatcat ggtctttgga tttattatct catatccgct gccatttata ccataagaac
     7141 tataatctat ctgatcaaga ttaagttttt ctactactac tgggttaatt tcttttaacc
     7201 aattatgtat tgtaggtttt ttagttgtgt atttatagtt tattttcaca gagtctttct
     7261 ttacatcaac gaaattcatc tgcttaagat attttaatat atcaccgtat agattaagtg
     7321 aggatatagt ataaccaacc gttccatctt ttactgattt ccctacaaat tttgttatat
     7381 tgttttgcca gtcatcaaat tcttttttct tcctcacctc ggtgttaaat acttttacag
     7441 caagatgttg tttatcggga tgtatagttt tcttaagatt attgagtaat gaatcaagcc
     7501 cttctatcga tagaaggttg cctttattgt ctgtaatata cgtcattaca tttccaatca
     7561 atacctttcc agcaggtgat tctaatcttt taccattttc aaatgactcc gctaaaataa
     7621 cttttcttat aattttgaat ccattcaaat ttttagtaaa tattgtttca gtattaattt
     7681 tatttgactg tattttattc tttttgccat ctatataata aaccctgttt gattttccat
     7741 aacttattac tttataccca tcaggaggat tgaaggtaaa agtgactgat tcggacagta
     7801 ctatttgagg atataagaaa cagagtaatg atagtactgt gaatagttta atcattatac
     7861 tatcactcag cagtactctt tgcagataga tatgtgttat agagaagcat tgctattgtc
     7921 attgggccca ctcctccggg aaccggagtg atgtaagatg ctttttcgct cactgcgtcg
     7981 aagtccacgt cacctgtgag cttgccgtca tcttttctat tgattcctac atcaatcaca
     8041 acagcaccgt cttttaccat atcaccggtt atgaagtcag cccttcctat tgcggcaaca
     8101 agaatatcag ccctgtttgc aacttccttt aagtcctttg ttctcgaatg acagattgtt
     8161 acggtaccgt gcctgtgaag aaggagcata gatacaggtt ttcctacgat gttgcttcta
     8221 cctaccacta caacctcttt gccctttata tcaagattgt aatgttccag cattcttata
     8281 attccagcag gtgtgcatga aacaaacctg gggttacctt ctactagcag gcccacattg
     8341 tagggatgaa acccgtctac atcttttaga ggagacacgg tgcgcaggat tgt
//
LOCUS       DBNGSV010000076        14767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1469061, whole genome shotgun sequence.
ACCESSION   DBNGSV010000076 DBNGSV010000000
VERSION     DBNGSV010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14767
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1469061"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC159U_02715"
     CDS             complement(<1..670)
                     /locus_tag="AC159U_02715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3336000.1"
                     /translation="MEQDVVSLVIQWLKESSHIIILTGAELSMDSGIPDFADSKFNPN
                     IREFRQKPEVREQYWQKIAKYYQALAQAVPGDGHKAIRELEVLCNVDYLFTQCADGLH
                     QKAGSGSVVELLGTMKWISCPNCGQDHKLEQVMGQLEDGKKIPVCDQCSSDLIKPPIS
                     FPGQPLPHWELREAWMKLHNSDLFLIIGVSLDDSPLASLQTIAAESGSKIVIINERES
                     EADNY"
     gene            917..1687
                     /gene="tolQ"
                     /locus_tag="AC159U_02720"
     CDS             917..1687
                     /gene="tolQ"
                     /locus_tag="AC159U_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR3336001.1"
                     /translation="MLNYQYWISIIGQVTESAPAHQVKISTLISTAGPVVKFVLLLLL
                     LMSLISWMIIFSKYLMVKSAKNKSEKFLDLYHTSGNFGNLFTSTKHIGGPLAEMFRAG
                     YTELLKIKNSKAKPEMMEEDINEAESIAPNLDNIVIIDRVIKKTMSSEISKLEGSLTF
                     LATTGSTAPFIGLFGTVWGIMNSFIGLSSSQDVPTLQAVAPGIAEALIATAIGLAAAI
                     PAVVAYNYFVSKVRAISIEMDNYLAEFLNVAERYISKL"
     gene            1696..2127
                     /gene="tolR"
                     /locus_tag="AC159U_02725"
     CDS             1696..2127
                     /gene="tolR"
                     /locus_tag="AC159U_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR3336002.1"
                     /translation="MGISSNNKNNDRLLSEINVTPFVDVMLVLLVIFMVTAPILYQGV
                     DVNLPKTSSEPIPQKSQKNIIVTVDKEGKIFIEKEQITLRELRVRAKNISRQSGNKEK
                     EEIFLRADELASYGVVMRVMSEIKNGGLEKVNLITDPYEEK"
     gene            2133..3053
                     /gene="tolA"
                     /locus_tag="AC159U_02730"
     CDS             2133..3053
                     /gene="tolA"
                     /locus_tag="AC159U_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR3336003.1"
                     /translation="MAKKEKKSKPTLFFGFILSAGLHLFIVATVLFWGFNSPALIKQP
                     KSITANLVSLPSAGSSGQRGSSGPKKEAAPKPPPPKKEIKPEIVEKKPEKVKIPEVVK
                     KPPKKVDPPKPKPEKKNTVNLNDKKTVKKQPKPTPKPVKKVVKKTPSAEQKRKKIMEE
                     MLAKAEKKERQQTRSDVLENLKKSSTKQVAKAGSDGYDITGAPTGSNSASAGLFVERV
                     RQEIASNWNMPPNIPTDGSLESKVLFKINASGEVFGIRIIKSSGNSAFDEFCKRALKK
                     ASPLDIPPPPEIQREAEIEGVEVTFNNSSY"
     gene            3082..3693
                     /locus_tag="AC159U_02735"
     CDS             3082..3693
                     /locus_tag="AC159U_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3336004.1"
                     /translation="MNLILIRHGETEWNKQGLCQGISDINLSEFGKVQASHLSHSLKD
                     QKIDSIYSSNLKRAYETAELIAENHGVKVKVEHGLREMDQGDFEGKPFVKLRETHGEV
                     LQSWRADPANFRIPNGETLQEVQDRAFNVIKNISINNEGKTVLIVSHNFTIITLLCKF
                     QGTSLKDFYKYKIKETSKHIINLNENRYTVETFNNTDHLSNIN"
     gene            3731..5395
                     /gene="argS"
                     /locus_tag="AC159U_02740"
     CDS             3731..5395
                     /gene="argS"
                     /locus_tag="AC159U_02740"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3336005.1"
                     /translation="MKESLKDIVRESVSSLYFEGSSKIPVSDIEISIPKKREFGDYSS
                     NIAMLLTKPLKKKPREIAEQISENLSTSHADLFKKVEIAGPGFLNFFINESSLMTELQ
                     IIEQQTDKFGHLDSVQGQKIIVEFVSANPTGFLHLGHARNAVVGDAISNILKASGFDV
                     TKEFYINDAGRQIEMLGTSVLCKYKEIFGIESEFPEDGYKGSYISDIANEINEQIGDK
                     LLKEKSEQSALEYCRNFAKDRLLQDIKDDLGKAGIYFDNWYSEKENLHGKNNKISQII
                     DSLEKNKALENKDGALWFKATEFGEQQDWVLIKSDGSPTYFIADIAYHNEKIERGFNR
                     LINIWGADHHSHISRLKASIKALGHDESILDVPLIQFVRLVSEGKEVKMSKRSGEYVT
                     MREVLEEVGADVLRFFMLMRSSDSHLDFDLDLAKRQSSENPVYYVQYAHARINSVFVK
                     AKEAGIGLSKDYINLLDKPEEVEIAKTLLNFPDVIKDSAYSLSPHKITFYLQEVASQF
                     HVYYNKYKVVTDDEKLSSSRLYLINCVKTVIHNGLKILGVSAPERM"
     gene            5401..6096
                     /locus_tag="AC159U_02745"
     CDS             5401..6096
                     /locus_tag="AC159U_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3336006.1"
                     /translation="MSETKSPTNRQIIFLFLGFLVLFILVFSLGVIVGKGLGDPEIKV
                     AKKQTAAQAPVKEEPEIENMQDAQNDQEISQQEMKDILEEAKTDIPKDSEQIKESDTI
                     QIETTDSEKKIAMNAQSEVNLKVEKPVLPKKENTSLLTKNTPLPPIVAGGQYTIQIGS
                     FKNESDAKSLQNKLKSRGYPAFLKQVTIGEDTWFRLRIGTFETKDQAKIYGDILLKKE
                     PELVKSVYATLNA"
     gene            complement(6147..7565)
                     /locus_tag="AC159U_02750"
     CDS             complement(6147..7565)
                     /locus_tag="AC159U_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336007.1"
                     /translation="MQKARVMVVEDESIVAKNIQTRLKQLGYVVPLTVSSGEEALEKI
                     ESVKPNIVLMDVVLDGKMDGIETAEIIYNKYKTPVVYLTAYADDETLERAKISTPFGY
                     IVKPFEVKELKSVIEIALSSYNTHTELKETSERLQSTFDTLEGCILVIDSKGIITFAN
                     KQSEIALMLDRDQLLGSELDKIIKIPFENSTQEIFSKNNHLHTELVTVNKPNQPICID
                     CSPINNDKGSFLGSLIKFQIASENADVSCIRNNQIQNIHSSADVISVGILASPKLILE
                     GIKNIVEKQQDFNIVFEETEAANLIDQIKQNEPDIVCIDNNVPDLDITTVLDYIDNNS
                     YSAKVILLLNKFNNAFLINTLTSGVRGCLTSSSESVQLVEAIRTVHNGNIWLEIDTLN
                     QILPKMVMQTKKKKHSLGSHNLTKREEEIARLILKGNSNKMIASKLYITEKTVKTHLT
                     KIFKKLGITNRLELAVKFNAQA"
     gene            complement(7587..9344)
                     /locus_tag="AC159U_02755"
     CDS             complement(7587..9344)
                     /locus_tag="AC159U_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HIR3336008.1"
                     /translation="METIIEDSDKTRLQLVKEIEVLKNRVSDLEKVKPDKEQSESALI
                     KSEEKYRSLFENSPSMFFTVKADGNIAEVNEFGCKELGYSSDELIGKPVINIFHPLDR
                     ETVSNQLQMCFDNPGKFFDWEFRKNKKNGETIWVRETARSFYSKDDGLIALIDCRDIS
                     VLKQAEERLNQTVEKLRIKTKYEELINSISQVIHRSINLNDVLDNTVQAIKGSIDIID
                     KVSIYFVEGNNAVLKAHSGLPSWYVEKTHTIPFDDGYTWNTIATGKPAYISDTDNDKL
                     VGPAEILLGIKSYVSVPIKFDNNIVGAINAASFRKDVFRDEDVSLLGLVANQISMVIE
                     NGRQSEQLQQTYNILEERVKERTLELLKSNALLREEIEKRIRAEVEIKKSLQEKEILL
                     KEVQHRVKNNLQVISSMLDLQTDYVKDSGVSEMFIEAQKRVKSMALVHEQMYQSEILT
                     DLDFSQYIENLGNYLFRIYGVNTKVITLETKIEEVNIDFNRAILLGLIVNELISNSLK
                     YAFSEDQKGYVRVRLDTEDDCYILTTSDNGKGLPKNFRLRQTKSLGLQLVQALTNQLK
                     GSIKIDRRKGTKFTIKFPK"
     gene            9518..11446
                     /locus_tag="AC159U_02760"
     CDS             9518..11446
                     /locus_tag="AC159U_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3336009.1"
                     /translation="MRDFGIALKLALLIIPITVAIFLFILGYYYNISKNTIINNVEEN
                     SKNLALNSVNKIEKSLKSFEIVAHNLSYQAAATDNTKDEILSFIENAVKNNNEVYGST
                     IAFEPYSFDTNSYYFAPYYYKNANKINFTTLGTSSYDYFKWDWYKIPKLENSPSWSEP
                     YYDKGGGEILMSTYSVPFYKQSGMDKKFTGVVTTDVSLKWLNSIVKSLKISQSGYGFI
                     LSKSGKFITHPNSNLIMNESIFSIAENKKSPKLKELGDKMISGRSGFELTRGILTDKS
                     FWIYYLPLPTNGWALGVMFPEDEIMADIKSLSRDAVLISIEGILILLLVIIYISRSIT
                     KPLRQLSEATKDIASGNLEFDIPKIGSQDEVGRLSKSFEYMRSSLKQYISDLKESTAA
                     TQRIESELKIARDIQMGILPKEFPPFPQRNEFEIFASIEPAREVGGDFYDFFFLDDNK
                     LFFIIGDVSDKGVPASLFMAVTKTLIKAVATTDRELHEIVYKINNELYSDNEMSMFVS
                     IFFGILDTGNGEITYTNAGHNAPVIIKSSGDVSFIEKTEGMALGAFENAKYSINKVIL
                     DNNDIIFLYTDGVTEAANSKSELFGEQNLVRTLKQYSNLDAKGVIIEIEQVVKSFAGS
                     EPQSDDITMLTIKYLRKS"
     gene            11450..11863
                     /locus_tag="AC159U_02765"
     CDS             11450..11863
                     /locus_tag="AC159U_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3336010.1"
                     /translation="MLEIELKNKVSELGKLHDKIAEFWNENNLDTDKMCHINLSLEEI
                     ITNIIKYGYDDDSEHIIKIIIKLEDQSVINLEILDDGIEFNPFDHPEPDTSIPLEQRE
                     IGGLGIHLVRNCMDHYSYERNDNLNKIVLIKNIGQ"
     gene            11885..12217
                     /locus_tag="AC159U_02770"
     CDS             11885..12217
                     /locus_tag="AC159U_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3336011.1"
                     /translation="MEQGNENPENISLLRLQGKLDFTSATEFEQKFNIMLGEGGQKFI
                     LDFSDVEYMSSAGLRFLLIASKKTKGNVVLCSVKDSVKQVIEIAGFASMFNFAHNQNQ
                     AVEILTKD"
     gene            12218..12415
                     /locus_tag="AC159U_02775"
     CDS             12218..12415
                     /locus_tag="AC159U_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336012.1"
                     /translation="MYKVGDKFRLHLPIIGKKNRFDVGLIGEITEIFNRDTGRAYRVQ
                     FNDGRTALLPEIIIEESSTIL"
     gene            complement(12465..14114)
                     /locus_tag="AC159U_02780"
     CDS             complement(12465..14114)
                     /locus_tag="AC159U_02780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3336013.1"
                     /translation="MTGNIENEQSEIKIKPPVLVVDDDKNQLKVISGILSMEDLQPLC
                     FTNAADAIELCAKQSINVAIIDLSLPDCDGLDLLKQLKEINPDMKVIINTGYASLETA
                     MEAVNKEAFAYITKMGNVEELLSHVHRAFHDHLTRYNEQLAREVSSRTAELQRSNMEL
                     KYEITERRKMQEELIKARQLESLGILAGGIAHDFNNLLTAILGNISLSKLYSDKENEV
                     YKRLEEAEKASIRARDLTQQLLTFAKGGSPVKKPTDIKDLIVDSVTLALRGSKVKSEY
                     EFDADIYSIEADAGQIAQVVNNITINSKQSMPDGGVIRVNIKNINNPGKELVGLTKSS
                     YICISIADEGIGIPEAYISKIFDPYFTTKQDGHGLGLATSYSIVKKHEGLITVESETG
                     KGTNFKIYLPAQPDDADKVSKNYDSEETVLNGKGRILIMDDENGIREIVSAMLSRIGY
                     SVVCTKDNDEAIAEYKKAYDEEKSYDAVILDLTIPGGQGGHKTLEDLLKIDPDVKAIV
                     SSGYYNDPVMSEFRKYGFLGCVAKPFKTSELSEILSKVIKQ"
     gene            14319..14627
                     /gene="groES"
                     /locus_tag="AC159U_02785"
     CDS             14319..14627
                     /gene="groES"
                     /locus_tag="AC159U_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3336014.1"
                     /translation="MGTKALHEGFRPLHDKVLAKRLGSEEVTKGGIIIPETAQEKPQE
                     GEVIAVGTGRILPDGTVVPLEVKKGDKILFSKYGGNELNIEGDDYIILEENDILAVLK
                     "
BASE COUNT         4952 a         2714 c         2665 g         4436 t
ORIGIN      
        1 catagttatc cgcttcactc tctctttcat ttattattac aattttcgag ccgctttcag
       61 ctgcaatggt ttgcagtgag gcaagcggag aatcgtccag tgaaacccct atgataagaa
      121 ataaatcact gttatgcagt ttcatccacg cttctctcag ttcccagtgg ggaagaggct
      181 gtccgggaaa agaaatcggc ggttttatta aatcactgct gcactgatcg caaacaggaa
      241 tttttttccc atcctcaagc tgtcccataa cctgctccag cttatgatcc tgaccacagt
      301 ttggacaaga tatccacttc atggttccca aaagctcaac aactgagcca cttcctgctt
      361 tttggtgaag accgtctgca cactgagtaa acagatagtc tacgttacat aaaacttcaa
      421 gctctcttat ggctttatgc ccatcacctg gaacagcctg ggcaagagcc tggtaatatt
      481 tcgcaatttt ttgccagtat tgttctctta cctcgggctt ttgccggaac tctctaatat
      541 taggattaaa cttagaatca gcaaaatcag gtattcccga atccatacta agctcagcgc
      601 ctgtgagtat gataatatgt gaagattcct taagccactg tattactaag cttactacat
      661 cctgttccac gataatggat tatacaatca gtattaaata agtgtcaatc aatcatgata
      721 atatgcctgc aattggacag aatttgagag cgattttaag aaatattgag tttaccttat
      781 atgagtcttg aaaatatccc taaatgtaat gaaatatttc taatctttta ttgattttag
      841 aataaagtgt gtaaaaattt aactaaaccg gaacttttta tcttgccgtt cgtaaaacaa
      901 ttataaggat cacaatatgc taaattatca atactggata tccatcattg gccaggttac
      961 agaatctgca cctgcgcatc aggtaaaaat ctctacttta atatccacag caggacctgt
     1021 tgtaaaattt gtactgctgc tgctgctttt gatgtctctt atatcgtgga tgataatatt
     1081 ttctaaatat ttaatggtaa agagcgctaa aaataaatca gagaagtttt tggacctcta
     1141 tcatacaagc ggcaattttg gaaacctttt tacttctaca aaacatatag gcgggccctt
     1201 ggcagagatg tttagggcgg gatatacaga acttcttaaa ataaaaaatt ccaaggccaa
     1261 acctgaaatg atggaagagg acattaacga agccgaaagc atagcaccca accttgataa
     1321 cattgtaatc atcgacaggg taattaaaaa aacaatgtct tcagaaatat ccaagctcga
     1381 aggatcgctt acatttttag caaccactgg aagcacggct ccctttatag gactttttgg
     1441 aacagtttgg ggaataatga attcatttat cggtctttca agcagtcagg atgtacccac
     1501 cctgcaggca gtagcacccg gtattgcaga ggctctaatt gctactgcca ttggtcttgc
     1561 tgcggctatt cccgctgtag tagcttacaa ttattttgta agtaaagtaa gagcaatatc
     1621 aattgagatg gataactatc ttgcagaatt tttaaatgta gctgagaggt atatttctaa
     1681 attataagat aagatatggg aataagctca aacaataaaa acaacgaccg tcttctttct
     1741 gagataaatg taactccctt tgtggatgtt atgctcgtcc ttcttgtaat atttatggtt
     1801 actgcaccca ttttatacca gggggttgat gtaaatctgc caaaaacatc ttcggagccc
     1861 attccacaga aatcacaaaa aaatattata gttaccgtgg acaaagaagg taaaatattt
     1921 atagaaaaag aacagataac actcagagaa ttaagagtga gagcaaagaa tatatccaga
     1981 caatcgggaa ataaggaaaa agaagaaata tttctcaggg cagatgaact tgcaagttac
     2041 ggtgtagtta tgagggtaat gtctgaaatt aaaaacggcg ggcttgaaaa ggtaaatctc
     2101 ataacagatc cctatgaaga aaaataaaat ttatggcaaa aaaagaaaaa aaatcaaaac
     2161 ccactctgtt ttttggattc atcttatcag ccggcttgca cctttttatc gttgccacag
     2221 ttcttttttg gggatttaat tccccggctt taattaaaca accaaaatca atcactgcta
     2281 atcttgtaag tcttccaagc gcaggtagtt caggacaaag aggatcatcg ggtcctaaaa
     2341 aagaggctgc accaaaacca ccgcctccca aaaaagaaat aaaacccgaa atagtggaaa
     2401 agaaaccaga aaaagttaaa atacctgaag tagtaaaaaa accaccaaag aaagtagatc
     2461 ccccaaaacc aaaacctgag aaaaagaata ctgttaactt aaatgataag aaaaccgtaa
     2521 aaaaacagcc aaaaccaact cctaaacctg taaaaaaagt agttaagaaa actccaagcg
     2581 ctgaacaaaa gcgcaagaag ataatggaag agatgcttgc aaaagctgag aaaaaagaaa
     2641 gacagcaaac cagaagtgat gtattggaaa atctaaagaa aagcagcacc aagcaagtcg
     2701 caaaagcagg ctctgatggt tatgacataa ctggcgcacc aactggttcc aattcagcat
     2761 cagccggact ctttgttgaa agggtaagac aggagattgc aagtaactgg aatatgcccc
     2821 ccaacatacc aactgacggc tcactagaaa gtaaagtatt gtttaagatt aatgctagtg
     2881 gagaggtatt tggaattaga attataaaaa gttctggcaa cagcgcattt gatgaatttt
     2941 gtaaaagagc tcttaaaaag gcatccccac tggatatacc gccgccgccg gaaatccagc
     3001 gcgaagcaga aatcgaaggc gttgaagtaa catttaataa tagctcatac taatagattt
     3061 aaaagatact atagcagata aatgaacctc atactgatca ggcacggaga gacggaatgg
     3121 aacaagcagg gactatgtca ggggatatcg gatattaatt taagcgagtt tggaaaggta
     3181 caggcaagcc atctttcaca ttcactaaaa gatcaaaaaa tagactccat atattcaagc
     3241 aacctgaaaa gagcatatga gactgctgag ctaattgcag aaaatcacgg tgttaaggta
     3301 aaagtagaac acggactaag agaaatggat cagggggatt ttgaaggaaa gccatttgta
     3361 aaattaagag agacccatgg agaagttcta cagtcctgga gagccgaccc cgctaatttt
     3421 cgcattccca atggagaaac cctgcaggaa gtacaggaca gggccttcaa tgtaataaaa
     3481 aatatcagta ttaacaatga aggaaagaca gtacttattg taagccacaa ctttactatt
     3541 atcactcttc tctgtaagtt tcagggcact agccttaaag atttttacaa atacaaaata
     3601 aaagaaacat ccaagcacat aataaactta aatgagaaca ggtacactgt agaaactttt
     3661 aataacaccg atcatttaag caacataaat taaacaatac tgttagtaaa caaagcaatt
     3721 tcacaaaaaa atgaaagaat cattaaaaga tatcgtaaga gagagtgtaa gcagcctgta
     3781 tttcgaaggc tcttctaaaa tacctgtatc agacatagaa atctccatac ccaaaaaaag
     3841 agagtttggt gattactcat caaacattgc aatgcttctt acaaaacctc taaagaaaaa
     3901 accaagagaa attgcagaac aaatttctga aaacctgagc acttcccatg cagatttatt
     3961 taaaaaagta gagatagcag gcccggggtt tctgaacttt tttataaacg aaagctcatt
     4021 gatgacagaa ttgcagatta tagaacaaca gacagataag tttggtcatt tagattcagt
     4081 acaaggacag aaaataatag ttgagtttgt gagtgcaaac cccacaggat ttcttcacct
     4141 tggacatgca agaaacgccg ttgttggcga cgccatatcc aatattctaa aagcaagtgg
     4201 ttttgatgtt acaaaagagt tctatataaa cgacgcagga agacagatag aaatgctggg
     4261 tacatcagtg ctctgtaagt acaaagaaat ctttggtata gaatcagagt ttcctgaaga
     4321 cggttataaa ggaagttata tatcagacat agcaaatgaa atcaatgaac agattggaga
     4381 taaattatta aaagagaaat cagaacaatc cgcactagaa tattgcagga actttgctaa
     4441 agacaggctg ctgcaagaca taaaagatga tctggggaag gccgggatat attttgataa
     4501 ctggtacagt gaaaaagaaa atcttcacgg aaagaataat aagataagcc aaataattga
     4561 tagtctagaa aaaaacaagg cacttgagaa taaagacgga gcgctttggt ttaaggctac
     4621 cgaatttgga gagcagcagg actgggtgct tataaaaagt gatggctcac cgacctactt
     4681 tatagccgat attgcctatc ataacgagaa aatagaacgc ggttttaata gattaataaa
     4741 catctgggga gcggaccatc acagccacat cagcaggctt aaggcttcta tcaaggccct
     4801 tggccatgat gaatcaattc ttgacgtacc gcttatacag tttgtaagac tcgtaagtga
     4861 aggcaaggaa gtcaagatgt ccaagagatc gggtgaatat gtaaccatga gagaagtact
     4921 tgaagaagtg ggagcagatg tactaaggtt ttttatgctt atgagaagct cagacagcca
     4981 cctggatttt gaccttgatc ttgcaaaaag acagtcgagc gaaaaccctg tttattatgt
     5041 gcagtatgcc catgcaagga taaacagcgt atttgttaaa gctaaagagg caggtatagg
     5101 attatcaaaa gactatatta atcttctgga taagcctgaa gaggtggaaa tcgccaaaac
     5161 tctgctaaat ttccctgatg ttattaaaga cagtgcctat agtttatcgc cacataaaat
     5221 aactttttac cttcaggagg tagcatctca gttccatgta tattataata agtataaggt
     5281 tgttacagat gatgaaaagt taagtagttc cagactatac ctaataaact gtgtaaaaac
     5341 cgttattcac aacggattaa aaatacttgg cgtatcagcg cctgagagga tgtaataatt
     5401 atgagtgaaa caaaaagccc aacaaaccga cagattatat ttttatttct aggtttttta
     5461 gtactgttta ttttagtctt ttcccttgga gtaatagtag gtaagggact cggtgatccg
     5521 gaaatcaaag tagcaaagaa gcagactgct gctcaagcac cggtaaagga agagcctgaa
     5581 atcgaaaaca tgcaggatgc tcaaaacgat caggaaatat ctcagcagga aatgaaagac
     5641 attttggaag aagccaaaac tgatattccc aaagactcag agcagataaa ggaatcagac
     5701 accattcaga ttgaaactac ggacagcgaa aagaaaatcg caatgaatgc tcagtcagaa
     5761 gtaaatctta aagtagaaaa accagtctta cccaaaaaag aaaatacttc acttctaaca
     5821 aagaatactc cccttccccc aatagttgca ggaggtcaat acactattca gatcggctca
     5881 tttaaaaacg agtcagatgc aaagagtctt cagaataagc ttaaatccag gggataccca
     5941 gcttttttaa aacaggtgac cataggagaa gacacatggt tcagacttag aattggaact
     6001 tttgaaacaa aggatcaggc aaagatctat ggagatattt tacttaaaaa agaacctgag
     6061 cttgtaaaat cggtatatgc tactttaaat gcctgataaa atacgattaa caatatttta
     6121 tagtgagcgt ttttacaact ttacaactaa gcctgagcgt tgaatttaac tgcgagttca
     6181 agtctgtttg tgattccaag cttcttaaat atcttggtta ggtgcgtctt tacagttttt
     6241 tcagtaatgt agagcttgct ggctatcatc ttgttgctat ttcctttaag aatcagtctt
     6301 gcaatctctt cttccctttt tgtaagatta tggctaccaa gtgaatgttt ctttttcttg
     6361 gtctgcatga ccattttggg aaggatctga ttcaatgtat caatctcaag ccagatattg
     6421 ccattatgta cagttcttat ggcctctacg agctgaacag actccgatga acttgtgagg
     6481 caacctctta cccctgatgt aagcgtgtta attaaaaacg cattattgaa tttatttaac
     6541 agcaggatca ctttggctga ataactgttg ttatcaatgt aatccagtac agttgttata
     6601 tccagatcgg gtacattatt gtctatacat acaatatcag gttcattttg tttaatctga
     6661 tcaattagat tagcagcctc tgtttcttca aaaacaatgt taaaatcctg ctgcttttcc
     6721 actatgtttt ttataccttc taaaataagc tttggtgaag caagtattcc tacagatatt
     6781 acatctgctg aagaatggat attttgtatt tgattattcc ttatacaaga tacatctgca
     6841 ttttctgatg caatctgaaa ttttattagt gaacctaaaa atgagccttt atcattattt
     6901 attgggctac aatctatgca gattggctga tttggtttat taacggtaac aagctcagtg
     6961 tgaaggtgat tatttttact aaaaatttcc tgtgtggagt tttcaaaagg aatttttatt
     7021 attttatcaa gctcacttcc cagcagctga tccctatcaa gcataagagc tatttcggac
     7081 tgcttattag caaaagtaat aatacccttg gagtctatta cgagaataca gccttccaaa
     7141 gtatcaaaag tactttgaag cctttcgcta gtctctttga gttcagtatg tgtgttataa
     7201 ctactaagag ctatttctat aacagattta agctctttaa cctcaaatgg ctttactata
     7261 taaccaaacg gcgtacttat ctttgctctt tcaagggttt catcatctgc ataagctgta
     7321 agatatacaa ccggtgtttt atatttatta tatataattt ctgcagtctc aataccatcc
     7381 atcttcccgt ctaatacaac atccataaga acaatattgg gctttacact ctcaattttt
     7441 tctaacgcct cctcacctga tgaaactgta agcggaacaa cataaccaag ctgctttaat
     7501 cttgtttgta tattctttgc tacaatgctt tcatcttcca ctaccattac tcttgccttt
     7561 tgcatgaatg ttactcccgg aattttctat ttaggaaact tgatagtaaa cttagtcccc
     7621 ttccttctgt cgatttttat tgaaccctta agctgatttg taagagcctg tactaattgt
     7681 aaacccaaag acttcgtttg tctaagtcta aagtttttag gaagtccttt tccattatca
     7741 gatgtagtta aaatatagca atcatcttca gtatcaagcc ttacccgcac ataccctttc
     7801 tgatcttcag aaaatgcata ctttagcgaa ttggatataa gctcatttac tataagacca
     7861 agcaatatgg ctctgttgaa atcaatattc acttcctcta tctttgtttc taatgtaatt
     7921 acttttgtat ttactccata tattctgaat aggtaattgc caaggttttc tatatattga
     7981 gagaaatcca aatccgtaag aatctcagac tgatacatct gctcatgcac aagagccatg
     8041 gactttactc gtttctgagc ttctataaac atctctgaaa ctccgctgtc ctttacatag
     8101 tctgtctgca gatcgagcat actggatatt acctgcagat tgtttttaac cctgtgttgt
     8161 acctctttta atagtatttc tttttcctgg agtgattttt taatctctac ctctgcacgt
     8221 attcttttct ctatctcttc tctgagaagt gcatttgatt ttaaaagttc aagtgttctc
     8281 tctttaaccc tttcttcaag tatattataa gtctgctgga gctgttcaga ctgcctgccg
     8341 ttttctataa ccatagatat ctgattggcc acaagcccca gaagagatac atcctcatcc
     8401 ctgaatacat cttttctaaa cgaagctgcg tttattgctc caacaatatt attatcaaat
     8461 ttgattggta cggatacata actttttatg ccaagcagaa tttcagctgg tcctaccagt
     8521 ttatcattat ccgtatcact aatgtaagcc ggtttacccg ttgcgattgt gttccatgta
     8581 taaccgtcgt caaaaggtat ggtatgtgtt ttttctacgt accagctggg caaaccgcta
     8641 tgtgctttaa gcacagcgtt attaccttca acaaaatata tagagacctt atcaattata
     8701 tcaatgctgc ctttaattgc ctgcacggtg ttgtctagta catcatttaa atttattgac
     8761 ctgtgtatta cctgagatat gctgtttatt agctcttcat atttagtctt aatcctcagc
     8821 ttttctacgg tttgattgag tcgttcttct gcctgtttta atacagatat atctctgcag
     8881 tcaataaggg caattaatcc atcatcttta gagtagaaag atcttgctgt ttctcttacc
     8941 catattgttt caccattttt tttgttcttt ctaaattccc agtcaaaaaa cttaccggga
     9001 ttatcaaaac acatctgaag ctgattggaa acagtctctc tatcgagtgg gtgaaaaatg
     9061 tttattactg gtttgccgat taattcatct gaagaataac caagctcctt acacccaaat
     9121 tcgtttactt cggcgatatt gccgtcagct tttacggtaa aaaacattga aggactattt
     9181 tcaaatagtg acctgtattt ttcttcactt ttgataagcg cactttctga ttgctcttta
     9241 tcgggtttta ctttttccag atcagaaaca cggtttttta gtacttcaat ctctttaaca
     9301 agctgtagcc gggttttatc tgaatcttca attattgttt ccatatacct gcttaatcta
     9361 ttatcattag ttagaaaatt ttataaacaa agatatattc atattataac tcaatttaat
     9421 attaatttga tccttacaat taatacatac aaaatagata cctaacaact ataaatcata
     9481 aataaattta gggcagtaca aactaactac taacgatatg cgtgatttcg gaattgcact
     9541 aaaacttgca cttttaataa tacctataac agtagcaatt tttctcttta tacttggata
     9601 ttattataat atttctaaaa atacaataat taataacgtc gaagaaaatt caaagaatct
     9661 ggcattaaac tctgtaaata aaattgaaaa atcactcaaa tcatttgaga ttgttgcgca
     9721 taacctgtcc taccaggccg ccgcaacaga caacaccaag gatgaaattc taagctttat
     9781 tgaaaatgca gtaaaaaata ataatgaggt ttatggttct acaattgcgt ttgaaccata
     9841 cagcttcgat actaactctt attactttgc tccctattac tataagaatg caaataaaat
     9901 taattttacg acacttggta caagttctta tgactatttt aagtgggact ggtataaaat
     9961 cccaaaactg gaaaacagcc cctcctggag tgaaccttac tatgataaag ggggtggaga
    10021 aatcctcatg tccacctatt ctgtcccttt ttataaacaa tcgggtatgg ataaaaagtt
    10081 tacaggagta gtaacaacag atgtttcact taaatggtta aacagtattg ttaaatcact
    10141 aaaaatttcc cagtccggct atggatttat tctttctaag agtggcaaat tcattactca
    10201 cccaaatagc aatctaataa tgaatgaatc aatatttagc atagcggaga acaaaaagtc
    10261 tccaaaacta aaagaacttg gagataaaat gatctctggc agatctggct tcgaacttac
    10321 aaggggaata ctaacagata aaagtttctg gatttattat ttgccgcttc caacaaatgg
    10381 ctgggcactt ggggtaatgt ttccagaaga cgagataatg gcagatatta aaagccttag
    10441 cagggatgca gtgcttataa gcattgaagg aattctgatc cttttacttg tgataattta
    10501 tatttccaga tccatcacaa aaccacttag acaactttcg gaagcgacta aagatattgc
    10561 atcgggaaat ctggaatttg atattcctaa aataggatct caggatgagg taggaaggct
    10621 atccaaatct tttgaataca tgagatcttc actaaaacag tatataagtg atttaaaaga
    10681 atccactgct gcaacccaga gaatagaaag tgagctaaag atagcaagag atatccagat
    10741 gggtattcta cctaaggagt ttccgccttt tcctcaaaga aatgaatttg aaatatttgc
    10801 ttctatagaa cctgcaagag aggttggtgg ggacttttat gattttttct tcttagatga
    10861 taataaactc ttttttatta tcggtgatgt ttctgataaa ggggttcctg catctctatt
    10921 tatggccgtt acaaaaacac taattaaagc agttgcaaca accgatagag agcttcatga
    10981 aattgtgtat aaaataaata atgagcttta ttcagataac gaaatgtcca tgttcgtaag
    11041 tatatttttt ggtattcttg atacaggaaa cggtgagata acatatacca atgcaggtca
    11101 taatgctcct gtaataataa agagttcagg ggatgtatcg tttatagaaa agactgaggg
    11161 tatggctctt ggagcttttg aaaatgcgaa atatagtatt aataaggtaa tattagataa
    11221 taatgatata attttcctct ataccgatgg tgttacagag gctgctaact ctaagagcga
    11281 gttatttggt gagcagaatc tggttagaac acttaagcaa tattctaact tagatgctaa
    11341 aggagtaata attgagatag agcaggttgt aaagtcattt gcaggatccg agccgcaatc
    11401 tgatgatata actatgctga caatcaagta tctcagaaaa tcatagatca tgcttgaaat
    11461 tgagcttaaa aacaaagtat ccgaacttgg aaaactccac gacaagattg cagaattttg
    11521 gaacgaaaac aatctggata ctgacaaaat gtgccatatt aatttaagtc tggaagaaat
    11581 tattaccaat atcattaagt atggatatga tgatgattcc gagcatatta taaaaattat
    11641 tattaagcta gaggatcaaa gtgtaattaa cttagagata ctggatgacg gtattgagtt
    11701 caatcctttc gatcaccctg aaccggacac atccataccc ctagagcaga gagaaatagg
    11761 tggtcttgga attcacctcg taagaaactg catggatcat tacagctatg agagaaatga
    11821 caatctgaat aaaatagttc taattaaaaa tatcggccaa tagattgtaa taaggagaaa
    11881 taaaatggaa caaggtaatg aaaatccaga aaacatctcg cttttaagac tacagggcaa
    11941 gcttgatttt acatctgcaa ctgaatttga gcagaagttt aatatcatgc tcggcgaagg
    12001 cggtcagaag tttattctag actttagtga tgtggaatat atgagcagcg ccgggcttag
    12061 gtttttgctc atcgcatcaa aaaaaacaaa aggaaacgta gtgctttgca gtgtaaaaga
    12121 ctctgtaaaa caggtaatag aaattgcagg gtttgcttct atgtttaatt ttgcacataa
    12181 ccaaaaccag gctgtagaga ttctaactaa agattaaatg tataaagtag gtgacaaatt
    12241 cagattacat ctacccataa taggcaagaa aaaccggttt gatgtgggtt taataggtga
    12301 aataactgaa attttcaaca gagataccgg tagggcatac agagtacagt ttaacgatgg
    12361 cagaacagcg ctactacctg aaattattat agaagaaagt tctacgatat tataaaatgc
    12421 gatcaaaaat ttgttctata ttgttggtat aataaaacat aattttactg tttaatcact
    12481 ttagataaaa tttcacttag ctctgatgtc ttaaacggtt ttgccacaca tcccaaaaaa
    12541 ccatactttc taaattcaga cataaccgga tcattgtaat acccgcttga aacaatcgcc
    12601 ttgacatcag ggtctatctt caaaagatct tcaagggttt tatgacctcc ctgcccccct
    12661 ggaattgtaa gatccaatat tactgcatca tatgattttt cttcatcata ggctttcttg
    12721 tattccgcaa ttgcttcgtc gttatctttt gtgcagacta ctgaatatcc tattctgcta
    12781 agcatggcgc ttacaatctc tcttatacca ttttcatcat ccatgatgag tattctacct
    12841 ttgccattta gcaccgtttc ttcggaatca taatttttgg aaactttgtc agcatcatct
    12901 ggctgagcag gcagatatat tttaaaatta gtgccttttc cagtctctga ttccactgtg
    12961 ataagtcctt catgtttctt cactattgaa taactcgtag caagtcccag cccgtgtccg
    13021 tcttgtttgg tggtaaaata cggatcaaaa attttagata tatatgcctc aggtattcca
    13081 attccctcat cagcaataga aatacagata taactactct tggtgagccc cacaagctcc
    13141 ttgcctggat tgttaatatt tttaatatta actcttataa cacctccgtc tggcatagat
    13201 tgtttggagt ttatggtgat gttatttact acctgtgcaa tctgccccgc gtcggcttct
    13261 attgaatata tatctgcatc aaattcatat tcactcttga cttttgatcc cctgagcgca
    13321 agtgtgacgg aatccactat taaatccttt atatccgtgg gcttttttac aggagaccca
    13381 cctttggcaa atgtcagaag ctgctgtgtg aggtctcttg cccttataga agccttctct
    13441 gcttcttcga gtcttttata tacctcattt tctttatcag aatacagttt ggaaagtgaa
    13501 atattgccca atatagcggt aagcaagtta ttaaaatcat gagcgatgcc tcctgcaaga
    13561 ataccaagag actcaagctg gcgggctttg atgagctcct cctgcatctt ccttctttcg
    13621 gtaatctcat attttagctc catattcgat ctctgaagct ccgctgttct tgagctgact
    13681 tcccttgcaa gctgctcatt gtatctggtc aggtgatcat gaaacgcacg gtgaacatgt
    13741 gagagcagct cctccacatt tcccatcttt gtaatatatg caaaggcttc tttattaaca
    13801 gcctccatag cagtctccaa agatgcataa cctgtgttaa taataacttt catatcaggg
    13861 tttatttctt ttagctgctt taacaaatcg aggccatcac aatctggtag agacaaatca
    13921 atgatagcaa cattgataga ttgttttgca caaagctcaa ttgcatctgc agcatttgta
    13981 aaacatagag gctgaagatc ttccatggat aaaatgccag aaattacttt aagctggttc
    14041 ttatcatcat caacaaccag cacaggaggc tttatcttga tctctgattg ctcattttca
    14101 atgtttccag tcataattat tacatttaat agataaactt ttatataatt ataaacccta
    14161 ttagttttat cacataataa tccatatttc tattattgcc cccttgacac ataacaaatc
    14221 atgcttatta tagtagcact ctaacattca gagtgctaaa aatataataa acataagacc
    14281 ataaaacata acaataattc ataaaaggag agaagaacat gggcactaaa gctttacacg
    14341 aagggtttag acctttacac gataaggtat tggccaagag gcttggaagt gaagaagtaa
    14401 caaagggcgg gataataatt cccgagactg cacaggaaaa acctcaggaa ggcgaagtaa
    14461 ttgcagtggg tacaggaaga attttgcctg acgggactgt tgttccactt gaagtcaaaa
    14521 agggagataa aatccttttt agtaaatatg gtggtaacga attaaatata gagggtgatg
    14581 attacattat ccttgaagaa aatgacatcc tggcagtact aaagtaaaaa aaggaacaga
    14641 aaaaaaatta ggaggcaata atcaacatgg gagcaaaaga gataaaattt aacagagaag
    14701 cccaggacct gatactgcaa ggtgtaaatg aacttgcaaa cgcagtaaaa tccacccttg
    14761 ggccaag
//
LOCUS       DBNGSV010000077         6861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1745927, whole genome shotgun sequence.
ACCESSION   DBNGSV010000077 DBNGSV010000000
VERSION     DBNGSV010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1745927"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /gene="lon"
                     /locus_tag="AC159U_02790"
     CDS             complement(<1..2138)
                     /gene="lon"
                     /locus_tag="AC159U_02790"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3336015.1"
                     /translation="MSLISLGDNNKVEIPLLPLRDVVVFPYMIAPLYIGRDKSIKAVN
                     AAMKNKKELFLVTQKDPKNEDPKEGDLYSVGTIATVAQMLKLPDGTVKVLVEGKKRGK
                     LVDFIPKSEYFLVHAEPIKEEQVSDIESEALIRNLLSAFESYVKHNKKIPAESYNNVS
                     SIKEANRLSDSICAYIQLKQDDKQELLEIIDPKQRLEKIFEKLSEDLEILKVEKRIEQ
                     RVKKRMEKAQKEHYLNEQMKAIQDELGEGDDMKSEVQEFEEKLKEKSLPEDVEEKIKK
                     ELKKLKLMSPMTPEANVERNYIDWILTLPWGDEKTEDRLDIDDATKILDEDHFGLDKP
                     KERIVEYLAVRKLTNKLKGPILCFVGPPGVGKTSLAKSIARAMDRKFVRISLGGVRDE
                     AEIRGHRRTYIGALPGKIIQGMKKAGTSNPVFLLDEIDKLGSDFRGDPSSALLEALDP
                     EQNKTFNDHYIEVDYDLSNVLFICTANVLHSIPWALQDRMEIIRIPGYTEIEKVKILK
                     HFLLPKQLEAHGLKDKNVTVTEGAILNAIRLYTREAGVRTIERELATLCRKAAKEIVQ
                     KGEDHSVKITPKNLNKFLGIPKFKFGEIEDTDIVGMSTGLAWSEIGGEILNIEVSIVP
                     GKGNFKVTGKLGDVMQESAQAAMSYVRSRTLQLGLERNFYQKVDIHIHVPEGAIPKDG
                     PSAGIAITTAIVSALIKKPVKRDVAMTGEIT"
     gene            2311..2988
                     /locus_tag="AC159U_02795"
     CDS             2311..2988
                     /locus_tag="AC159U_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3336016.1"
                     /translation="MSNTKINRRNFIKQGLTGFGIVLSGQILLNTEAVPTEKSGDLDE
                     FLALSTDKLPKLKPTEDNILGPFHVNGAPFRGKVSHPAAKGEILLIKGRVFGFDTKRP
                     LVNTSIDIWQADSEGNYDYGQNTTKTYYYRARLITDENGNYEYETIHPGAYKLGQNFW
                     RPPHIHYLIRHENYKTLITQLYFSGDPHQKDDRFVKQSLIIDLQQVTSKQGSYKLGVF
                     DIVLEPE"
     gene            complement(3187..3426)
                     /locus_tag="AC159U_02800"
     CDS             complement(3187..3426)
                     /locus_tag="AC159U_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336017.1"
                     /translation="MEILVENIYIQIIQGIVFGLLLGLVPLYIANKRGQTNIGIWAVV
                     LCSVASSIFGPVSAMPIAAIFIMIASLKETETTKE"
     gene            complement(3498..3863)
                     /locus_tag="AC159U_02805"
     CDS             complement(3498..3863)
                     /locus_tag="AC159U_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR3336018.1"
                     /translation="MKEQPVYFFDTEYNRNSSTNIDVIIDHVTKKEHNYNVVLLDDDD
                     HTYDYVIEMLMNIFGHSIQESYLMACEVDFKGRVIVCTTSKDDAEKKKEEILNFGPDY
                     RLSRSTGSMNAIIEPAENE"
     gene            4002..4838
                     /locus_tag="AC159U_02810"
     CDS             4002..4838
                     /locus_tag="AC159U_02810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3336019.1"
                     /translation="MKISLYGKNVILTGASSGIGWQTALLLAEQGANIALAARSMDKL
                     GELEKELSKFDVKVLKVQTDISKITDCKKLIREAVNGLGSIDILVNNAGVTSRGKLDD
                     VDLNDLEQMIEINLKAPVRITKLALPHILKSDSGRIINVASILGIIPLPTEAVYSATK
                     FALRGFSYALAEELEDTGVKVCLICPGPVETPMIIDEMDKISDMVYSPPVSTAKEIAE
                     LIVKSAVDGSMERIKPIHTGILAKIGFLFPKISKLVKPIMEKQGADRKKKYIDKYSKS
                     SD"
     gene            4860..5141
                     /locus_tag="AC159U_02815"
     CDS             4860..5141
                     /locus_tag="AC159U_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336020.1"
                     /translation="MSNENDADNKFSSNQKALAYLDKLLVSRDFDPDDREYIRFTLIP
                     MLTSVIENMDDGFAEELRYMAMDLCMKLIPADEQYQRFVEDWIKKKKVV"
     gene            complement(5175..5780)
                     /locus_tag="AC159U_02820"
     CDS             complement(5175..5780)
                     /locus_tag="AC159U_02820"
                     /inference="COORDINATES: protein motif:HMM:NF044451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3336021.1"
                     /translation="MDKKQLSLKIQQGRETRIKLIEAAIEVFSKDGYKNASMELIVEI
                     TGVTRGALYYHFENKLVLFEAVFDNIQAQIADNILDAANKEPDKWNQLIAGCRAFLES
                     CIDPKIQRIAIIDAPSVLDWNTWRKVDSVHGLSLLKSTLSDLRNDGVIETGSLDALAH
                     LLSGAMNEAALWIIQSENHKKSLDESMEMIEMMLNSFKVNK"
     gene            5910..6314
                     /locus_tag="AC159U_02825"
     CDS             5910..6314
                     /locus_tag="AC159U_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3336022.1"
                     /translation="MKISSMYPVICTDKVEESKEFYTTNFPFEISFETDWYLSLAAKE
                     SDFELAIVRYNHPSVPTNFRKQAQGMILNFEVEDVDSEYERFKEQNVEIIQNLKDEEW
                     GQRHFIISDPNGILIDIIEMIEPSIEYLNDYN"
     gene            complement(6345..>6861)
                     /locus_tag="AC159U_02830"
     CDS             complement(6345..>6861)
                     /locus_tag="AC159U_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR3336023.1"
                     /translation="KTYALLVLQNEEGKLYQKFDVIIIENKHKLKEVNMIRILVILLM
                     GLSTIWVEISQAENQITVKSMPPVVVKTFPEAGDTAVDPSIKEIRVTFSKDMMTEQMW
                     SWVMASKDSFPVITGDVKYLEDKRTCVAPVSLEPGKSYAIWFNSQNHNAFRDTDNNPA
                     VPYLLVFETSK"
BASE COUNT         2089 a         1250 c         1231 g         2291 t
ORIGIN      
        1 gtgatttctc cagtcattgc aacatcgcgt tttacaggtt ttttaatcag agcagatacg
       61 attgctgttg tgatcgcaat acctgccgag gggccgtctt ttggaattgc accctctgga
      121 acatgaatat gaatgtccac tttttgatag aaattccttt caagcccaag ctgaagggtt
      181 cttgacctca cgtaactcat tgctgcctga gctgattcct gcataacgtc acccagtttt
      241 ccggtgacct taaagttgcc cttacccgga actatagaaa cctctatgtt aagaatctct
      301 ccgcctatct ctgaccatgc aagacctgta gacataccta caatgtcagt gtcttcaatc
      361 tcaccgaatt taaattttgg tattcctaag aacttattca gattcttagg cgtaattttc
      421 acgctgtgat cttcaccttt ttgaactatc tcttttgcag cctttctgca aagcgtggca
      481 agctctcgtt ctatggttct tacaccggct tctctggtgt agagccttat ggcattaaga
      541 atagcacctt cggtgactgt tacattcttg tctttaagcc catgtgcctc aagctgtttt
      601 ggaagcagaa agtgctttag gatttttact ttctcaatct ctgtatagcc aggaatacgg
      661 attatttcca ttctgtcctg aagtgcccat ggtatagaat ggagcacatt ggctgtacag
      721 ataaaaagca cgtttgaaag atcataatcc acttcaatgt aatggtcatt aaatgttttg
      781 ttttgttcag gatcaagcgc ttctaagagt gcagaagaag gatcacccct gaaatctgat
      841 ccaagcttgt ctatttcgtc caacaggaat acagggttgc tggttcctgc ttttttcata
      901 ccctgaataa tctttccggg aagcgctccg atgtatgttc ttctgtgacc acgaatctcg
      961 gcttcgtctc ttacacctcc aagagatata cgcacaaact tcctgtccat tgctcgtgct
     1021 atggactttg caagtgaagt cttacctaca ccaggtgggc caacaaaaca aagtatcggg
     1081 ccttttagtt tatttgtaag ttttctaaca gccaggtatt cgacaatcct ttcttttggc
     1141 ttatcaagac caaagtgatc ttcatccaaa attttagtag catcatcaat gtccaacctg
     1201 tcttcagtct tttcgtcacc ccatggtaga gtcaaaatcc agtctatata gtttctttca
     1261 acgtttgcct caggagtcat aggtgacatg agttttaatt tcttaagctc tttctttatc
     1321 ttttcttcta cgtcctctgg cagcgacttt tcttttaatt tctcttcaaa ctcctgtacc
     1381 tctgatttca tgtcgtcgcc ttcaccaagc tcgtcctgaa tggctttcat ctgctcattt
     1441 agataatgct ctttttgagc cttttccatt cttttcttta ctctctgctc gatacgtttt
     1501 tcaaccttaa gaatttctaa atcttcgctg agcttctcaa atatcttctc gagcctttgt
     1561 ttaggatcta tgatttcaag aagttcctgt ttgtcgtcct gttttagctg aatataggcg
     1621 catattgaat cagaaagtct gtttgcttcc ttaattgagg aaacattgtt ataagattct
     1681 gcaggtattt tcttgttatg tttaacataa ctttcaaaag ccgaaagcaa atttctgata
     1741 agtgcctcgg attctatgtc tgaaacctgt tcttctttga ttggttcagc gtgtacgaga
     1801 aaatattcag actttggaat aaaatcgaca agctttcccc tttttttacc ttctaccagt
     1861 accttaacag taccatctgg cagctttagc atctgcgcaa ctgtggcaat tgtaccaaca
     1921 gagtatagat cgccttcttt ggggtcttcg ttctttggat ccttttgagt aaccagaaat
     1981 aattcttttt tgtttttcat ggctgcattt acagccttta tggatttatc tcttccaata
     2041 tacaaaggtg ctatcatata tgggaaaaca actacatccc ttaggggaag caggggaatt
     2101 tcaactttat tgttatcgcc caagcttata agtgacattt aattacctcg ctcatttatt
     2161 atatcatata tatttagatt aatcttagcc tatttagaag caatatatat actaaatatt
     2221 tatttaatta tgattttttt tatttcaaga ggataatatt ataagaaaaa ttagtttatt
     2281 attaatgttt ataaattata ctttaatttt atgtctaata ctaagataaa tagaagaaat
     2341 tttataaaac aggggcttac agggtttgga attgtgctga gtggacagat tttattgaat
     2401 actgaagcag ttcctactga aaaaagtggc gatcttgatg aatttttagc gttatcaact
     2461 gataaactgc caaaacttaa accaactgaa gataatattc tagggccttt tcatgtaaat
     2521 ggggcaccgt ttaggggaaa agtttcccat cccgcagcaa aaggagaaat tttacttata
     2581 aagggcaggg tttttggttt tgatacaaaa agacctctgg taaacacatc aatagacata
     2641 tggcaggccg atagtgaagg aaattatgat tatgggcaga ataccactaa aacttactat
     2701 tatagggcaa gacttattac cgacgaaaat ggtaattatg agtatgaaac aattcatccc
     2761 ggggcttaca aacttgggca aaacttctgg aggcctcccc atattcacta ccttataaga
     2821 catgaaaatt ataaaacact aattacacag ctctatttta gtggtgatcc acatcaaaaa
     2881 gatgatagat ttgttaaaca atctctaatt atcgatctgc agcaagtaac cagtaagcaa
     2941 ggaagctata agcttggagt gtttgatatt gtgcttgaac cggagtaatt aggtgaaaat
     3001 agatgaattt cctctctggc ataggggggt ggccaaaaag gaaattcatc agttccgttt
     3061 agaggacttt aacatgcata gtcatatata taatatgcaa attgcatgcc aaaatgtggg
     3121 attttgatgg aattagttta tatattatag taggggttta tttaataaac cagcaaaaaa
     3181 atattcttat tcttttgtag tttcggtctc ttttaatgaa gctatcatta taaaaattgc
     3241 ggcaatgggc attgcagaca ctggcccaaa aattgaactt gctactgagc ataatacaac
     3301 agcccagatg ccaatattag tctggcccct tttattggct atatatagag gtacaagacc
     3361 tagtaaaagg ccaaagacta tgccctggat tatttggata tatatatttt caactagaat
     3421 ttccatttaa tgttgcacta tattaatctg atttagacta ctattttatt taaattatta
     3481 attaaagtaa ataatatcta ctcattttca gcaggttcaa ttattgcatt cattgaaccc
     3541 gtgcttctgg ataacctgta atccggtcca aaatttaaga tttcttcttt tttcttttct
     3601 gcatcatctt ttgaagttgt acaaacaatt actcttccct tgaaatcaac ttcgcatgcc
     3661 ataaggtacg attcctgaat actatgacca aaaatgttca taagcatctc aattacataa
     3721 tcatatgtgt gatcatcatc atccagaagt acaacattgt aattatgctc tttttttgta
     3781 acatggtcga taattacatc aatatttgtt gatgaatttc tgttgtattc cgtatcaaaa
     3841 aaatatactg gctgttcttt catgaatcta atataaacca atctaaatac atttatcaat
     3901 atctaatagt ttagattaat tatgcatttt atactgttat ctattaataa tattaatgaa
     3961 aataattcag aatagtataa tataaattta aggtaaaaaa aatgaaaata tcattatatg
     4021 gtaaaaatgt aatcttaaca ggcgcatctt cgggtatagg ttggcaaact gcgctcttgt
     4081 tggctgagca gggggctaac attgctctgg cagcaagaag tatggacaaa ctgggtgaac
     4141 ttgaaaaaga actctctaaa tttgatgtga aagttttaaa ggttcaaaca gatatttcaa
     4201 agattactga ttgtaaaaaa ttgataagag aagctgtaaa tggtttgggg tcgatagata
     4261 tattagtaaa taatgcaggt gttacaagca ggggcaaatt ggatgatgta gatcttaacg
     4321 atttagagca aatgatagaa attaacctta aggctccggt tcgtataaca aaacttgccc
     4381 tcccacatat tttaaaatca gattcaggaa ggataataaa tgtagcgtca attcttggaa
     4441 ttatacctct tcctactgaa gccgtttatt ccgctacaaa atttgcactc agaggtttta
     4501 gctatgcgct agccgaagag cttgaagaca caggcgttaa ggtctgtctt atatgcccgg
     4561 ggcctgttga aactcccatg ataattgatg aaatggataa gatttccgat atggtttatt
     4621 ctccccctgt ttcaactgct aaagaaattg cagagcttat tgtaaaatct gcagtagatg
     4681 gcagtatgga aagaataaag cccattcaca caggaatatt agccaagata ggctttctgt
     4741 tccccaagat tagtaaattg gttaaaccga ttatggaaaa acaaggggct gacaggaaaa
     4801 agaaatatat tgataagtat tctaaatcat cggattaaat aagcgatcgg aggacaagaa
     4861 tgagcaacga aaatgatgca gataacaaat ttagcagtaa ccaaaaagca cttgcttatt
     4921 tagataagct tctcgtatct agggatttcg atcctgatga cagggagtat ataaggttta
     4981 ctcttatacc catgcttact agtgtaattg aaaacatgga tgacgggttt gccgaagagc
     5041 taagatatat ggccatggat ctttgtatga aacttattcc agccgatgaa cagtatcaga
     5101 gatttgtaga agactggatt aagaaaaaga aagtggtttg attgctggtt tgtataagat
     5161 ttcaaagcaa tagactactt attgacttta aaagaattga gcatcatttc gatcatttcc
     5221 attgattcat ctaatgattt cttgtggttt tctgattgaa ttatccaaag ggctgcctca
     5281 ttcattgcac cggaaagaag atgagctaga gcatctaagg atccggtctc tatgacccca
     5341 tcatttctca aatcgctcaa ggtggatttt agtagcgaaa gtccatgcac cgaatctacc
     5401 ttgcgccagg tgttccaatc aagcactgaa ggcgcatcta taatggctat tcgttgtatt
     5461 ttgggatcga tgcaagattc taaaaaagct ctgcagccgg cgatgagctg gttccattta
     5521 tccggttctt tgtttgcagc atctaaaata ttgtctgcaa tttgcgcctg tatattatca
     5581 aacacggctt caaaaagcac cagtttgttt tcaaagtgat aatagagcgc accacgagta
     5641 actcctgtaa tttccactat caattccata gaagcatttt tgtatccatc tttactgaaa
     5701 acctcgatag cggcttcaat taattttatc cttgtttctc ttccctgttg tatttttaaa
     5761 cttaattgct ttttatccat tatttataac ggggaaatac ttattattta ttatcctaat
     5821 cttaaatact tataaccatg tttgccttga tatatttaca tacatacagt atgtatgtat
     5881 aattttatta ataatattaa ggggttaata tgaaaatatc aagtatgtat ccggttattt
     5941 gtacggataa ggtagaggag tcaaaggagt tctatactac taattttcca tttgagataa
     6001 gctttgagac ggactggtac ttaagtttag ctgcgaaaga aagtgatttt gaattagcta
     6061 tagtgcgata taaccacccc agtgtcccga ctaatttcag gaaacaagct caggggatga
     6121 tactaaattt tgaggttgaa gatgtagatt cggagtatga acgatttaaa gagcaaaatg
     6181 ttgaaattat acaaaaccta aaagatgaag agtgggggca gagacacttc atcatttcag
     6241 acccaaacgg gattttgatt gatataatag aaatgataga accttcgata gagtatttga
     6301 atgattataa ttagataagt ttttgaaatt gtaaatttaa aattttactt actggtctca
     6361 aaaaccagga ggtacggaac cgcaggatta ttatccgtgt ctctgaatgc attatgattt
     6421 tgcgagttaa accagattgc atagctcttt ccgggctcta gactaacagg tgctacacat
     6481 gttctcttat cttctaaata tttaacatcc cctgtaatta ctggaaacga atccttagat
     6541 gccattaccc atgaccacat ctgctcagtc atcatatcct tgctgaatgt gacccttatc
     6601 tcttttatgg aaggatcaac ggcagtgtcc cctgcttcag gaaatgtttt tacaacaaca
     6661 ggcggcatgg atttaaccgt aatttgattt tctgcctgag atatttcaac ccagattgtc
     6721 gacagtccca taagtaatat tactaaaatc cgtatcatat taacctcctt taatttatgc
     6781 ttgttctcaa taattatgac atcaaatttc tgatataact tcccctcttc attctgcagt
     6841 accagtaatg cataagtttt t
//
LOCUS       DBNGSV010000078         1920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_291473, whole genome shotgun sequence.
ACCESSION   DBNGSV010000078 DBNGSV010000000
VERSION     DBNGSV010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_291473"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1722
                     /locus_tag="AC159U_02835"
     CDS             259..1722
                     /locus_tag="AC159U_02835"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HIR3336024.1"
                     /translation="MMGKQSNNQQKLFHYNVSLEQRIPQKHQLRKIRELIDFDFVYHE
                     VKDCYGDTGNVSVPPPVILKMMLLLVAYNVRSERELMDTIPFRLDWLWFLGYDLDDEI
                     PNHSVLSKARTRWGVHTFKLFFERVVWQCVEAGLVEGSKLFMDSSLIDANASNNSVVD
                     KQQVKRHLSESYQIFEERLDAVVDEKTTPANSRYVSSTDPDASVTRQGSGRSKLRYKT
                     HRGVDAQCEVITTTRVTPGSRDDGALFEEMIEFHEQTTHTSVTTAVADSKYGTIENFL
                     TSYDRGIKAHMPSIEETHRGAGRRKDIFPKEDFTYNPDDDTFSCPAGNILKRRNYSKE
                     RQQYEYKASSKVCACCQLRADCTRAKNGRTLKRHIRQHALDSVLTIAHGQDAKRDLKT
                     RMHLSERSFAHAGRYGYKRARWRRLWRMEIQDFLIATIQNIQILIKHMNPRVKSGVST
                     LGNHPRFTDGIQRIYSLISPAASHLFNTLSLNHSLSF"
BASE COUNT          590 a          426 c          418 g          486 t
ORIGIN      
        1 aaaattgcca aagaaaataa ctttacaggc tttacagcag agattctcca tgaaaataag
       61 aatatgctgc aagttatcca taacagttca tataaaatta ccagcaaatt tgaggaaggt
      121 gtttaccata tttctttccc ctttgtggat ggctaggggt ctaaactaaa cgggttgttg
      181 cccaaatcct tttactgaca agagataatc tggtacaatc tcctttaaga tactcacttt
      241 agggagaaca gtatcattat gatgggcaaa caatctaata atcagcaaaa gctttttcac
      301 tataatgtga gcctcgaaca acgaatccct caaaagcacc aactccgtaa gatacgggag
      361 ctgatagact ttgattttgt ttatcatgag gtaaaagact gctacggtga cactggcaat
      421 gtttcggtgc ccccaccggt cattctcaaa atgatgctat tattagtagc ctataatgtt
      481 cgctcagagc gagaactgat ggatactatt cctttccgtt tagattggct atggttttta
      541 ggatacgatc tggacgatga gattcctaac cacagtgtgc tcagtaaagc ccgaacgcgc
      601 tggggagtcc acacattcaa gttatttttt gaacgagtag tatggcaatg cgttgaggct
      661 gggctggttg aaggaagtaa actcttcatg gattcaagtc tcatcgatgc taatgcctct
      721 aataactcag tagtagataa acagcaggtg aaacgccacc tcagtgaaag ttatcagata
      781 tttgaagaac ggcttgatgc agtagtggat gaaaagacca caccggccaa cagtcgctat
      841 gtatcgagca ccgatcctga tgcgtcagtc acgcgtcagg gcagtggcag atcaaaacta
      901 cgctataaga cacatcgagg ggtagatgct caatgtgagg taattaccac aaccagagtt
      961 acccctggat cccgagatga tggtgccctt tttgaagaaa tgatcgaatt ccatgagcag
     1021 accacacata caagtgttac caccgcagtt gctgacagca agtacggcac catagagaat
     1081 ttccttacaa gttatgatcg aggtatcaaa gctcacatgc cctccattga ggagacccac
     1141 cggggagctg gccgtcggaa ggatatcttt cccaaagaag acttcaccta taatccggat
     1201 gatgacacct ttagctgccc agcgggaaac atcctgaaaa gaaggaatta ttccaaagaa
     1261 cgacagcagt acgaatataa ggcttcttct aaagtctgtg catgttgtca actccgagca
     1321 gactgtactc gagccaaaaa tggacgaacg ttaaaacgac atatcagaca acatgcatta
     1381 gatagtgtgc tcaccatcgc tcacggccag gatgcgaaac gagatctaaa aaccagaatg
     1441 cacttatcag aacgctcatt tgcacacgca ggtcgttatg gctataagag agcccgatgg
     1501 agacgactgt ggagaatgga aattcaagat tttctcattg ccacgatcca aaacatccag
     1561 attcttatca agcatatgaa tccgagagtg aaaagcgggg taagcacatt aggtaatcac
     1621 ccacgtttta ctgacggcat tcaaagaatt tatagtttaa tttcccctgc tgcatcacat
     1681 ttatttaata cccttagcct gaaccactct ctatcttttt aaactagctt tgggcaacag
     1741 cccgtcaaga cctgacacct tttgctacag gagcacatct atgatagttg gcgaacaaaa
     1801 atccatagct gaaatcaaag agctggtggc atctttcacg aaggtcctta tcctgggctg
     1861 cggaacctgt gtcaagacct gttttgccgg gggagaagat gaggttgcca cccttaccgc
//
LOCUS       DBNGSV010000079         1282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_153563, whole genome shotgun sequence.
ACCESSION   DBNGSV010000079 DBNGSV010000000
VERSION     DBNGSV010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_153563"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1227
                     /locus_tag="AC159U_02840"
     CDS             join(80..341,341..1227)
                     /locus_tag="AC159U_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611759.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3336025.1"
                     /translation="MPRKRYTPEQIIRYLRHAEVLSSQSKSVSEICREIGVSENTYYR
                     WRKMYGGMEVDQARKLKELERENSRLKRVVADLTLDNLILKEAFRGKLLSTERRRKCV
                     VHIQQKLGISQRRACKVLGQSRSVQRHRSKRSLEQEVLRNDIVRLASKYGRYGYRRIT
                     ALLRAEGWKVNHKRVERIWREEGLRVPRKQPKRGRLYLNDGSCIRLRPCWKNNVWSYD
                     FVVDRLNNEKRIRMLTVIDEYTRKCLAIRVGHTIKAHHVLDTLSTLFITEGIPDYIRS
                     DNGSEFTASTLRQWLESIGVKTAYIEPGSPWENGYNESFNGKLRDELLNTEIFYTLKE
                     AQILIEQWRVHYNEVRPHSSLGYRPPAPKALIQDKKTTNYEQTFTPTH"
BASE COUNT          484 a          205 c          300 g          293 t
ORIGIN      
        1 tgaccttgcc ccatataata gtaccagatt ataagttaga attttaggta ataaacccta
       61 tgaataggag gcaagggaaa tgcccagaaa gcgttacaca ccagagcaga taataagata
      121 tttaagacat gcagaagttc ttagttcaca gagtaaaagc gtatcggaga tatgcagaga
      181 gataggagtg tctgaaaata cttattacag atggagaaag atgtacggag gcatggaagt
      241 agatcaggca agaaagctaa aagagcttga aagagaaaac tcaagattaa agagagtagt
      301 agcagacctt acacttgaca acctgatact caaagaggca tcagagggaa acttctaagc
      361 accgagcgca ggagaaagtg cgtagtacat atacagcaga aactgggaat atcacagaga
      421 agagcgtgta aagtactagg acaatcacgt tcggtgcaaa ggcatagatc aaagcggtct
      481 ttggagcagg aagttttaag aaatgatata gttcgtcttg ccagtaagta cggaaggtat
      541 ggatacaggc gtattacagc actgcttaga gctgaaggct ggaaagtaaa tcataagcga
      601 gtagaaagga tatggagaga ggaaggactt agagtaccca gaaaacagcc taaaagaggg
      661 agactatatc ttaatgatgg ttcttgtata agactcagac catgctggaa gaataatgta
      721 tggagctatg actttgtagt ggacagacta aacaatgaaa agaggataag gatgcttact
      781 gtgatagatg aatatacaag gaagtgtctt gcaataagag taggacatac aataaaagcc
      841 catcatgttt tggacactct tagcacttta tttataacag aaggaatccc tgattatatt
      901 aggagtgata acggaagtga gtttacagca agtactttaa ggcagtggct tgaaagcatt
      961 ggagtaaaga cagcatatat agaaccgggg tctccgtggg aaaacggata caatgagagt
     1021 tttaatggaa agctaagaga cgagcttctt aacacagaga tattttatac attaaaagaa
     1081 gctcaaatcc ttattgaaca atggagagtg cactacaatg aggtaagacc tcacagctct
     1141 cttggttaca gaccgcctgc tccaaaagct ttaatacaag acaaaaaaac cacgaactat
     1201 gaacaaactt tcactccaac tcactgacaa aatgttaaca aattctaact ttaaaactgg
     1261 acctaaattc tggggcttga ca
//
LOCUS       DBNGSV010000080         1282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_153633, whole genome shotgun sequence.
ACCESSION   DBNGSV010000080 DBNGSV010000000
VERSION     DBNGSV010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_153633"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1227
                     /locus_tag="AC159U_02845"
     CDS             join(80..344,344..1227)
                     /locus_tag="AC159U_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611759.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3336026.1"
                     /translation="MPRKRYTPEQIIRYLRHAEVLSSQNKSVSEICREIGITENTYYR
                     WRKMYGGMEVDQARRLKELERENSRLKRAVADLTLDNLILKEASGGKLLSTERMRRCI
                     IHIQQKLGISERRACKVLGQSRSVQRHKPNKSLEQEVLRKDIVRLASKYGRYGYRRIT
                     AMLKAEGWKVNHKRVERIWREEGLKVPKKQPKRGRLYLNDGSCIRLRTCWKNHVWSYD
                     FVTDRLNNGKRIRMLTVIDEYTRKCLAIRVGSSLKAHHVLDTLSTLFITEGIPDHIRS
                     DNGSEFTASTLRQWLQCIGVKTAYIEPASPWENGYNESFNGKLRDEFLNTEIFYTLKE
                     AQILIEQWRVYYNEVRPHSSLGYKPPAPRAVISDNKSKNYEQTCTSTQ"
BASE COUNT          493 a          204 c          286 g          299 t
ORIGIN      
        1 tgaccttccc ccctataata gtaccagatt acaagttaga attttaggta ataaacccta
       61 tgaataggag gcaagggaaa tgcccaggaa gcgttacacg ccagagcaaa taataagata
      121 tttaagacac gcagaagttc ttagttctca gaataaaagc gtatcggaga tatgcagaga
      181 gataggtatt acagagaaca cctattacag atggaggaag atgtacggtg ggatggaagt
      241 ggatcaggca agaaggctaa aagagcttga aagagaaaac tcaagactaa agagagcagt
      301 agcagacctc acacttgata acctaatact aaaagaggca tctgagggaa acttctaagc
      361 accgagcgta tgagaagatg cataatacat attcagcaga agctgggaat atcagagaga
      421 agagcgtgta aagtattagg acagtcacgt tcggtgcaga gacacaaacc aaacaagtca
      481 ttggaacaag aggttttaag aaaagatata gtgcgacttg ccagtaaata cggaagatat
      541 ggatacagac gcattactgc aatgcttaaa gcagaaggct ggaaagtaaa tcacaagaga
      601 gttgaaagaa tatggagaga ggaagggctt aaagtaccta aaaaacagcc taaaagaggg
      661 agactttatc taaatgatgg ctcatgtata aggctcagaa cgtgttggaa aaatcatgta
      721 tggagctatg actttgttac agacaggtta aacaatggaa agagaattag gatgcttacg
      781 gtaatagatg aatacacaag gaaatgtctt gcaataagag ttggatcaag tttaaaagct
      841 catcatgttc tggatacact tagcacttta tttattacag aaggtattcc tgatcatata
      901 agaagtgata acggcagtga atttactgca agtactttaa ggcaatggct tcaatgcata
      961 ggagtaaaaa cggcttatat agaacctgct tctccttggg aaaacggata caatgagagt
     1021 tttaatggaa agttaagaga tgagttttta aacactgaga tattttacac tttaaaagaa
     1081 gcacaaatac ttatagaaca atggagagta tattacaatg aagtaagacc acacagctca
     1141 cttggctaca aaccccctgc tccacgagct gttatatcag ataataaatc caaaaactat
     1201 gagcaaacat gtacttcaac tcaatgacaa aatatcagca aattctaact ctaaaggtgg
     1261 acctgaattc tggggcttga ca
//
LOCUS       DBNGSV010000081         6477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1331047, whole genome shotgun sequence.
ACCESSION   DBNGSV010000081 DBNGSV010000000
VERSION     DBNGSV010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1331047"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC159U_02850"
     CDS             complement(<1..326)
                     /locus_tag="AC159U_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3336027.1"
                     /translation="MKISLLGGTGDIAEGLVLRWSKAGHEIFIGSRSEEKAQGIAAEY
                     IDKLKELGIESKIHGLANADAARESEVIIISIPPEYAASTIEQCGSNITDQIVVTPVV
                     SMKKEG"
     gene            complement(413..820)
                     /locus_tag="AC159U_02855"
     CDS             complement(413..820)
                     /locus_tag="AC159U_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336028.1"
                     /translation="MRIAVLFVALTLFTLSSAFALDTSCNFCSKDSADFFNDNLTEQL
                     ITSAFTGNDTNILLASESSITDTNSHTVSGENNFKQFEAGSYNLDNIIELINKTEDIK
                     SFTQDKIDLLANFRDGDDVFVPLGFFLYMNKKF"
     gene            1021..1554
                     /locus_tag="AC159U_02860"
     CDS             1021..1554
                     /locus_tag="AC159U_02860"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3336029.1"
                     /translation="MKIIKWLFIIFVLFIVVFIAVGLMLPTQYSVSRSVDINSASDEI
                     HKYVGDLKMWDKWQPWTYGDPSLVIKTGEKTTGVGASQSWKGKDGSGELKFTKSDPKT
                     GIEYDMFFDEGKYKCKADMKYNGTGSTTEVIWTMSGNAEIPVLGGYFALLVTDGTGPM
                     FEKGLANLKTVVEKNKQ"
     gene            1573..2379
                     /locus_tag="AC159U_02865"
     CDS             1573..2379
                     /locus_tag="AC159U_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3336030.1"
                     /translation="MNYKELADTGVQISEIGLGTWNYRGWVKPLQRGIDLGATFIDTA
                     EGYYTEDIVGKAIRGQRNKVFIATKVSGRHLSYRDVLRSAEESLYKLGTDVIDLYQIH
                     WPNPAYPLKETMRAMQKLADDGLIKYIGVSNFSLDEMKEAQYYLSNHKIVSNQVLYNL
                     NHREIEKDLIPYCDTNKITIIAYTPLDSGNLTKKGLLNKKSNEVLEHVAASEGKTMSQ
                     VALNWCNSRKCVVSIPKSDSVERTEENCGGSGWKLQKENIDKLDQAFSGN"
     gene            2508..2942
                     /gene="arfB"
                     /locus_tag="AC159U_02870"
     CDS             2508..2942
                     /gene="arfB"
                     /locus_tag="AC159U_02870"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR3336031.1"
                     /translation="MNISQYNIHITPSLSVDLGELTFEFIKSSGPGGQNVNKVSTGVR
                     MRFDVLNSASLPSEVRSRLLKLNKNRINKEGFLVLESQKYRTQEANKKDLLNKFTELL
                     YEASRKPKKRVKTRPSKTSVQRRLDMKNKRSKLKQTRKKPEW"
     gene            2960..4291
                     /locus_tag="AC159U_02875"
     CDS             2960..4291
                     /locus_tag="AC159U_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3336032.1"
                     /translation="MDRISILRLFYYLLAFIVPPLTFSAVIIYKSISDISFSDPEPFD
                     VSTLQSPAIDPDKLTAVIVVGNKGTEITDFLAPYELLSLTGKFNVITVAPRRMVSPLN
                     GGIGFIPHFSLDQIDEVMNKNPDLVVVPNLPNIQSPEDKVLIDWIRNQDNGETVFLSI
                     CEGARTIAAAGLLANKKATTHWSAIGHLQGQYPGTEWLSGVRYVEDGNIISSPGVVTG
                     SVDGTLYTIEKMLGKNTATGVAKSIGYEHYGRDELEKVKINISDSVWLVTALYPWGKD
                     EIGVYIKDGISETDLASLLDVYPRSFTAVTYTVSNERKIVKSKHGLDFVPTFDFKTIG
                     YLDRMVSLSKDLEQEDKDFFAKYYKHLDIEYFHDLKRNASDFAYDRTLLDLSARENRV
                     VARSVSKTMEYPIDHLSLSGSGWPFHLLLGPILLGIGGVIFARWFEKRYLV"
     gene            4386..4601
                     /locus_tag="AC159U_02880"
     CDS             4386..4601
                     /locus_tag="AC159U_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336033.1"
                     /translation="MSENQEQTCKCGYTREHPWVVPKHKYSTWGMIVVSIGISYAPKL
                     MTIECDKCGEVFETIDDKETLLKNKYI"
     gene            complement(4695..5711)
                     /locus_tag="AC159U_02885"
     CDS             complement(4695..5711)
                     /locus_tag="AC159U_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336034.1"
                     /translation="MINKYNTDIKEQLSSDKDLKKFVSNPTIVPGYKVNILIDDTDVV
                     YDLKNGITWEFIARGAAGGVAGAIAGEVVVSMLKRTLGLNVNYEQLFNNAINEIVNQL
                     GRVIDNAFLREYLADIEHMRSELLTYAETKELRILHENREHASKIVHRLMGFNAQEGL
                     GGFLCAANLHLMVIKALAGHNGDYKKTLQRKCKDYAAWAEHSANLFLERVKTSVSPCE
                     SHCHEERDPGGGRLSLPLYEKTKSPCNCNNENEKNALNVLPRDNESLYVDTDDTVCSY
                     SFKHNYGRDERRFGPRYNYADCDRARQTYYDSAVNPATDRRNRILDICNLWRAVNIDD
                     IRTS"
     gene            complement(5958..>6477)
                     /locus_tag="AC159U_02890"
     CDS             complement(5958..>6477)
                     /locus_tag="AC159U_02890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336035.1"
                     /translation="DQRAQESGFAGRITTLEADMNALSFAKDSFDLIIAEGSAYIMGF
                     ANALKSWNPLLKTGGHIAVTELVWLTQTPPKEAAAFFNKEYPAMTTPENIIPIFKESG
                     YKLIDHFTIPDKGWWDEYYTPLAGRLNNLEEKYENDDPGRNIIQMTRLEIDIRKRFGK
                     SYGYEFFIAKKV"
BASE COUNT         2037 a         1136 c         1266 g         2038 t
ORIGIN      
        1 ttaccctctt ttttcataga aactacaggg gtaacaacta tctggtctgt aatgttagat
       61 ccacactgtt caatagttga agctgcatat tcaggtggga tgctgattat aattacttca
      121 gattctcttg ctgcatctgc atttgcaagt ccgtgaattt ttgattctat tccaagctct
      181 tttagtttat caatatattc tgctgcaata ccctgggctt tttcttcgct tcttgagcca
      241 ataaaaatct catgtcctgc ttttgaccat cttagtacca atccttcagc aatgtctcct
      301 gtaccgccta aaagtgatat tttcataatt taaagtactc ctatattttt ttgaggtgtt
      361 attgtaacgg attaatacaa agtagtaaat ctggaacagg aaaaatacga gtctagaact
      421 tcttattcat ataaagaaaa aatccgagcg gcacaaatac atcatcgcca tctctaaaat
      481 ttgcaagtaa gtctattttg tcctgtgtga atgatttaat gtcttcagtc ttatttataa
      541 gctcaattat gttatccaaa ttatatgaac cagcttcaaa ttgtttaaag ttgttctcgc
      601 cactaactgt atgagaattc gtatctgtta ttgatgattc agaggctaat aggatattgg
      661 tatcattgcc tgtaaatgct gatgtgatta attgttctgt taaattatca ttgaaaaagt
      721 ctgccgaatc ttttgaacaa aagttgcagg atgtatctag tgcaaatgca ctgctaagtg
      781 taaataatgt taatgctaca aatagtacag ctattctcat gttaatcccc ttcataaaac
      841 ttgcttcaat ttaaataaaa ttatatgcct tctagcttaa gataaatcat cctttaagaa
      901 aggttgaatc aaatctacgg ctgattctat acccaaaatg gtataaattc aagtatatat
      961 acgaaaaata tactatagta ttgtataatg tatttatact agcaatagag gtttagtgta
     1021 atgaaaataa taaaatggct atttataata tttgttctgt ttattgttgt atttattgcg
     1081 gtggggctta tgctgcctac acagtactcg gtgagcagat cggtagatat taattctgca
     1141 agcgatgaaa tacataagta tgttggtgat ctcaagatgt gggataaatg gcagccctgg
     1201 acttacggcg atccttcttt ggttattaaa acaggtgaaa aaacaaccgg agtgggtgca
     1261 tctcaaagct ggaaagggaa agatggatcg ggagaactca aatttacaaa gtctgatccc
     1321 aagaccggta ttgaatatga tatgttcttt gatgagggaa aatacaagtg taaggcggat
     1381 atgaagtaca acggtacagg tagcacaact gaggtaatct ggacaatgtc gggaaatgcg
     1441 gaaattccgg ttttaggcgg atattttgcc ttactggtta ctgatggaac agggcctatg
     1501 tttgaaaagg gtcttgcaaa tttaaaaaca gtagtagaaa aaaataagca gtaagaataa
     1561 gcaataatag agatgaatta taaggaacta gcagacacgg gagtacagat ttcagaaatc
     1621 gggcttggta cgtggaacta caggggctgg gttaagccgc ttcaaagagg tattgatctt
     1681 ggggcaacct tcatagacac tgccgagggc tattatactg aagacattgt gggaaaggcc
     1741 attcgggggc agagaaataa ggtattcata gctacaaaag tttcgggaag gcatctttct
     1801 tacagagatg tactgaggtc tgccgaagag agtctctaca agcttggtac cgacgtaatt
     1861 gatctctacc agattcactg gccaaacccg gcctatccac taaaggagac catgagggcg
     1921 atgcaaaagc ttgccgatga tggcctcatt aaatatatcg gtgtgagtaa tttttctctc
     1981 gatgagatga aagaagctca gtattattta agcaatcata agattgtttc taatcaggtt
     2041 ttgtataacc taaaccaccg tgagattgaa aaagacctta taccttactg tgatactaat
     2101 aaaataacta taattgcata tacgcctcta gattcgggca atcttactaa aaagggtctt
     2161 cttaataaga aatcaaatga agtgctggag catgttgcag cctctgaagg caagaccatg
     2221 agccaggtag ccctaaactg gtgtaattcc aggaaatgtg tggtttcaat tcccaaatca
     2281 gacagtgtgg agagaaccga agagaactgc ggaggctctg gctggaaact gcaaaaagaa
     2341 aatatagata agctggacca ggcttttagc ggcaattaac ttattaataa atatccagag
     2401 ctgtctgctg atttcgaagc ttatatctta tatatacttt caattccctt tattttattt
     2461 atgattgaac tttgttatag aaattatctc ttaagtattg gcttaaaatg aatatatctc
     2521 aatataatat acacataact ccttccctct cggtggattt aggtgagtta acatttgaat
     2581 tcataaagtc cagtggaccc ggagggcaga atgtaaataa agtatcaact ggtgtgcgga
     2641 tgagatttga tgttctaaat tctgcaagcc tgccttcaga agtaagatcc agacttttga
     2701 aactaaataa aaacagaata aataaagaag gtttcctggt gcttgaatcc cagaagtaca
     2761 gaacccagga agcaaataag aaagatttgc ttaataaatt tactgaactg ttatatgaag
     2821 caagccgcaa acctaaaaaa agagtaaaaa caagaccatc aaagacatct gtacaaagaa
     2881 gacttgatat gaagaataaa aggagcaaac tcaagcagac caggaaaaag cctgagtggt
     2941 agtttgagaa aagacatata tggatagaat aagcattctg agactgtttt attatctgct
     3001 tgcgtttata gtcccaccac ttacgtttag tgccgtaata atatataagt ctatttcaga
     3061 tatatcattt agtgatccgg agccatttga tgtatcaaca ctacagtctc ctgcaataga
     3121 tcctgataaa ttaacggcag taattgtggt tggtaataaa ggtactgaaa ttacagattt
     3181 tctggctcca tacgagcttt tatccctcac gggaaagttt aatgtcatta ccgtggcacc
     3241 gcggcgtatg gtttctccgc taaacggcgg aattggtttt atacctcatt tttctttaga
     3301 ccagatcgat gaggtcatga acaaaaaccc cgatcttgtt gttgttccta atcttcctaa
     3361 tatccaaagc cctgaggata aggtcttaat agactggatc agaaatcagg ataacggcga
     3421 gacagtattt ttatcaattt gcgaaggtgc caggacaatt gcggctgcgg ggcttcttgc
     3481 aaataaaaaa gctactaccc actggtcggc tattggtcat ttgcaggggc aatatccggg
     3541 cactgagtgg ctttcaggcg taaggtatgt tgaggacgga aatataattt catcacccgg
     3601 tgtcgtgaca ggctcagtag acggtacttt atatacaatt gaaaagatgc ttggaaaaaa
     3661 tacagctaca ggtgttgcta aatccattgg ctatgagcat tatgggcggg atgagcttga
     3721 gaaagtaaaa ataaatatct ccgattctgt ctggcttgtt acagctcttt atccctgggg
     3781 aaaggacgag ataggagttt atataaaaga cggtataagc gaaaccgatc tcgcttcatt
     3841 actcgatgta tatcccagat catttacggc ggttacttat acggtttcca atgaaagaaa
     3901 gatagttaaa tcaaagcacg ggctcgattt tgtacctact tttgatttta agacaattgg
     3961 ttatctggac aggatggtaa gtctttctaa agatttggaa caggaagaca aagatttttt
     4021 cgctaagtat tataaacatc tggatattga atattttcat gatctgaaac gcaatgcatc
     4081 agactttgcc tacgacagaa cactgctgga tttatcggca agagaaaacc gggtggttgc
     4141 aagatcagtc tctaaaacaa tggaataccc aatagatcat ttaagcctaa gcggtagcgg
     4201 ttggccgttt catttacttc ttggcccgat acttcttggt atagggggag taatatttgc
     4261 ccgctggttt gaaaagaggt atctggtata aattattaaa gaatcttatc aatatgaatt
     4321 atgtctaaaa tagataaatt gcaattactc ggtaaaatct aaccaaacaa tttaattaat
     4381 taaatatgtc agagaatcaa gaacaaacct gcaaatgcgg ctacacaaga gaacatccct
     4441 gggtagtgcc caaacacaaa tattcaacct gggggatgat tgtggtaagt ataggaatca
     4501 gctatgcccc taagctcatg actattgaat gtgataaatg cggggaagtg tttgagacca
     4561 tagacgataa ggaaacactt cttaagaata agtatattta atattatagt cttttggatt
     4621 gagtgggaat agaaaaggaa agagttccca ttttttgact attttttggt tctatttttt
     4681 gacatctttg gctcttaact agtacgaata tcatctatat tgactgccct ccatagatta
     4741 catatatcta atatcctatt tcttcgatct gttgcgggat ttacagctga gtcataatat
     4801 gtttgtctag ctctatcaca gtcagcataa ttgtatcttg gaccaaagcg tctttcatct
     4861 cttccataat tatgtttgaa tgaatatgaa catacagtat catctgtatc aacgtacaaa
     4921 ctttcattat ctcgaggcaa cacgttcagt gcattctttt cattttcatt attgcaattg
     4981 catggggatt tggttttttc gtataacggt agagacagac gaccccctcc gggatctcgt
     5041 tcttcatgac agtgtgattc acatggagaa acacttgtct ttaccctttc aagaaagaga
     5101 tttgcagaat gttcagccca agcagcataa tctttgcatt ttcgctgtaa tgttttctta
     5161 taatcaccat tatgtcctgc tagagcttta ataaccatta aatgtaaatt agctgcgcaa
     5221 agaaatccac caagtccttc ttgtgcatta aagcccatta gtctatgtac tattttagaa
     5281 gcgtgctctc tattttcatg aagaattcgc aattctttgg tttcagcata agttaataat
     5341 tcacttctca tatgttcaat atctgctaaa tattcacgta aaaaagcgtt atcaataact
     5401 cttccaagtt ggtttactat ttcatttatt gcgttattga acaactgttc atagttaaca
     5461 tttagcccca atgttctctt aagcatagac acaactactt caccagcgat agcacctgct
     5521 actcctccag cagctcccct agctatgaat tcccatgtaa ttccattttt taaatcataa
     5581 actacatccg tatcatcaat tagaatatta actttatatc caggtactat agtaggatta
     5641 gaaacaaatt tctttaaatc tttatctgat gataattgtt ctttaatgtc cgtattgtat
     5701 ttgttgatca ttacaagaac cctccatatt tacatttaat gtatcaatat accatagata
     5761 tataaaattg attaagccga ttgattgatg aaatggggga cgcttgtaga tgctcagtaa
     5821 ttaggtgcat tttttaatta agaaaaaagg gatgttaccc tttttgggga atgaacagat
     5881 gggaacagac ggtgtcaggc cttgacattg gacactattt gtttaaaaaa tagtgtcagt
     5941 catggtttta ttggaatcta tactttcttt gctataaaaa attcatagcc atagcttttc
     6001 ccaaaacgtt tgcgtatatc tatttccagc cgagtcattt gtattatgtt acgccctgga
     6061 tcatcatttt catacttttc ttctaaatta ttaagacgtc cagcaagagg ggtgtaatac
     6121 tcatcccacc atcctttgtc gggtatggta aagtgatcta ttagtttata acctgactct
     6181 ttaaatattg gtatgatgtt ttctggtgtt gtcattgccg gatactcttt attaaaaaat
     6241 gcggcggctt cttttggtgg agtctgtgta agccatacaa gctcagtaac ggcaatatga
     6301 ccgccagttt taagaagtgg gttccatgac tttaacgcat tagcaaaacc cattatataa
     6361 gctgaccctt cggctatgat taaatcgaaa ctatcttttg caaaagatag tgcattcatg
     6421 tctgcttcga gtgtggttat acgaccggca aatccagact cttgtgcccg ctggtcc
//
LOCUS       DBNGSV010000082         9461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_778002, whole genome shotgun sequence.
ACCESSION   DBNGSV010000082 DBNGSV010000000
VERSION     DBNGSV010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_778002"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC159U_02895"
     CDS             complement(<1..388)
                     /locus_tag="AC159U_02895"
                     /inference="COORDINATES: protein motif:HMM:NF021365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3336036.1"
                     /translation="MKRHVNHLIIFLKYPQIGKVKTRLAKDIGDVKALNIYKLLVSST
                     LNKVQSTSFDISIYYTPKDKDKEVRKFIDFNDVNYYSQTGNNLGARMLNAFSESFTAG
                     YKKTIIIGTDCPDINYNTISDTFDLLN"
     gene            complement(399..2945)
                     /locus_tag="AC159U_02900"
     CDS             complement(399..2945)
                     /locus_tag="AC159U_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3336037.1"
                     /translation="MDNLSNEILHAPSYSKLNSNNRIQIFIDPEGPNWISTDNKGAKI
                     LSIIDGKKTFDEVVRLYSTEQNIPFAKAWVDVDAFIKDSVREKFVSLTPYSRAPYKGR
                     SDYLQLTDLSELWIHTNNSCNLQCTHCLVESGPSGDRGLETSVLKDLIDQAKKLGVYR
                     FYFTGGEPFIRKDIFELIDYITTAPYTELIILTNGMFFNHKDNLKQFQKVSRGRVKPQ
                     ISLDGSTAKANDSIRGKGTFKKIIEGIKSAVKSGLDPTVTTVVNSDNLHDISNITRLI
                     HGLGVNNHHLLWVHQRGRISQNDSMIVPTYKLIEVLRETKSVADELGIKIDNFESIKT
                     KLKSKKFTKHDLSNACWKSLCVYSDGKVYPSAAFANYPGLSGGSILDNTLESIWKESP
                     VFNKFRTATVQKKTKCNNCYIKYICGGGDIEHTYFYTASTFGKGDLEGLDPYSELHEY
                     IINTSLYELAQESARKFNRASGFDSPIVYKSMGEEVLDCNINGNGNTNGNGLSSNGHP
                     NGIHNYTDLIIEVSHSNCVLTYDFDKSRESVRDFYGKAAEQPQADLCCPTSYPLDDTS
                     HIPQDVIDRFYGCGSPMNIAEIKEGEVVTDLGSGAGIDCFIASKKVGRKGKVFGIDMT
                     DNMLKVANECKSVVSKNLGFSNVEFKKGFLEEIPLENESVDLITSNCVINLSPNKKTV
                     FSEMWRILNDHGRIVISDIVSEKEVPPHIINNQQLWGECIAGALTEEQFISYLEQSGF
                     YGIQVLAKSYWKTIESYKFYSVSVRGYKFSKKAGCVYIGQKAIYHGPFKATLDEEGHI
                     FPRGEAVEVCTDTARKLSHHPYSSFFTITDPTDKVSDDFTCCSDSVTGCC"
     gene            3096..3764
                     /locus_tag="AC159U_02905"
     CDS             3096..3764
                     /locus_tag="AC159U_02905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3336038.1"
                     /translation="MIKAVIFDFDGVIADTEHLHFEVFRNVLNHQKQDLTENEYYSQY
                     LAYDDKTFFSKYYENNELKLDKKLLKRLLKNKSEEFDSLIKNNIKIYPGVVDFIEKLS
                     VRYDLAIGSGALKKEIVQILDVINIAHLFKIIISADEISNCKPNPEVYNTVLSRLNNI
                     KGNDISADECIVIEDSIYGIQAAKSAGMKCMAVTNSYPAKKLTEADLVVSDYSVSDIE
                     QISG"
     regulatory      4293..4379
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      4567..4671
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      4844..4947
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5252..6190
                     /locus_tag="AC159U_02910"
     CDS             5252..6190
                     /locus_tag="AC159U_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336039.1"
                     /translation="MYKIFTTFLCSIFACLIFISVNSVKADDCVAPVSMSCSYEETGD
                     TTIVATVTVSYLVDGSTASDLSSVVFSDNCDATAVGVGVEVVDPTHDTNADDTNPCAE
                     GDMKITVDDYDDFCTLATSATCSAGNIGAICSADTDCDTSDASGDGVCGDGICDATST
                     TATCSAGLVGIACSADVDCDDIGGDGVCGDSSCVAMVCVGGINDTQACASDVDCPDTL
                     SSTCEGGSDDTLKICTVNDDCADTQTAASIECIKTIDEIDISACDEEIATIAESSNLH
                     DRQVEDSKDCNGRRCKNDDDDKGHDRGTVLCTVPEV"
     gene            6308..6838
                     /locus_tag="AC159U_02915"
     CDS             6308..6838
                     /locus_tag="AC159U_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HIR3336040.1"
                     /translation="MDRRNFIKYISSTALLSFIVPINLSFGQSTYKDRVISKFIDLIL
                     PLNNSAILNVESFRSKLDQNLRNNKNAEKIIDYGIKFFDHNANLLYQKPQFLQLSKTE
                     QLEIINFTINNTVSLDKPDPSKPWTDIRYGKMFFNNLRSFVMNEFYSSPEGWKFIGYK
                     GPPQFGGNLDYTKCTY"
     gene            6839..8524
                     /locus_tag="AC159U_02920"
     CDS             6839..8524
                     /locus_tag="AC159U_02920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3336041.1"
                     /translation="MAKQTEYDVCIIGSGAGGAPTAYALGKAGFKTIVLEAGRRFKPE
                     EYHVNKKDWENYPYPQPFTDFKNRSKERYTSAPTEKLNLKYSHLRSKAKYRGQYNGSN
                     NRQRPFVQRAKGIGGTTLHYQAEAQRFSDYGFKTNSLFGFGEDWPITYKDLEPYYDKA
                     EKILNVSGQLGNPEKPKRIPFPQPAHELSCASKRVKVGFDKLGLSLLPNTLNILSKPA
                     DGRPACNYCNGCFLGCMMRAKGSMDVTFIPKAEATGNVTVQSNSAVREITVNAKGKAD
                     GVIYLDDSKTEHRIKSKIVVVSGGALETPRLLLNSKSSQFPDGLGNSSGLVGKNFMET
                     VVYINTAIYEEDIESYKGLQIDSRSYDYNKTSADRGFNGGVVFGLSAMDLLGPLGYAR
                     TIAPTWGDEHVKFMNDYFGHAINIFAIGEMIPRETNTITIDSSKTDYYGIPVAKIKTR
                     MSTNDLDMISFMQKKTREIIDSSGAKESVRELSSYDLSLISHIGGTTKMGTDPKKSVL
                     NAFSQSHDVKNLFVCDSSPFVSQGGGDSPSLTIHALALRASDYIISEAKKGNI"
     gene            8608..9396
                     /locus_tag="AC159U_02925"
     CDS             8608..9396
                     /locus_tag="AC159U_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336042.1"
                     /translation="MKKLLVVPLLFFVLGIQNSFAGGAPTPTPIPVPEGVACLQPTVD
                     GCAWFPDALMPGVTGDDDDSGPSISGYGYVSVPVVSDSNAKCVGDDDDCNGGINEGEA
                     CNDDMDCPIVGCINYPECATNKLAWEVDVHDTHSSFYDPNPCASTGAKLDFQCSDNIT
                     SIPEGCNFIQAPQPGLIGVDEIRCPVEFDGLSVGYCDLYNDDDDLIGPVFIDGPPPIG
                     VKLSGKHLNQQASEGRQRGGKNTSRNGTNQTGVAHCIPRFIIEN"
BASE COUNT         3002 a         1603 c         1776 g         3080 t
ORIGIN      
        1 tatttagtaa atcgaaagtg tctgagatgg tattgtaatt aatatcagga caatctgttc
       61 cgattataat tgttttctta taaccggctg taaaagattc actgaaggca tttaacattc
      121 tagcgcctaa attgtttccg gtttgtgaat aataatttac atcattaaaa tctataaact
      181 ttcgtacttc tttgtcttta tcttttggtg tgtaataaat agaaatatca aagctagtac
      241 tttgaacctt atttaaggta ctcgatacga gaagtttata tatattaagg gctttaacat
      301 ctccaatatc ttttgcaagc ctggtcttaa ccttaccaat ttgtggatat tttaaaaaaa
      361 taatcagatg attaacgtgt cttttcattt acagaccact aacagcatcc ggtaactgaa
      421 tcactgcagc atgtaaagtc atcggagacc ttatcagttg gatctgtaat agtaaaaaat
      481 gaactgtacg ggtgatgtga taacttgcgc gcagtgtcag tgcatacctc tactgcttca
      541 ccccttggga atatatgacc ttcttcgtcc aatgttgcct tgaaaggacc atgatatatg
      601 gctttttgac ctatgtagac acatcctgct tttttgctga atttataacc tcttactgat
      661 acagaataga acttatagct ctctatggtt ttccaataag actttgcaag cacctgtata
      721 ccataaaatc ccgattgctc caggtagctt ataaactgtt cctcagttaa tgcgcctgct
      781 atacactcac cccaaagctg ttgattatta attatatgag gaggcacttc tttctctgat
      841 acaatatcac ttatcactat acgtccgtga tcgtttagta ttctccacat ttctgaaaac
      901 acggtttttt tattaggtga gagatttatt acacagttcg atgtaattaa gtctacagat
      961 tcattttcaa gaggtatttc ttctaaaaat ccttttttaa attcaacatt gctgaatcca
     1021 agattttttg agacaacaga tttacattca tttgctactt ttagcatatt gtctgtcata
     1081 tcgattccaa acacctttcc cttccgacca actttttttg aagctataaa acagtcgatt
     1141 cctgctcccg agccaaggtc tgtaacaacc tctccctcct taatttcagc aatgttcatg
     1201 gggcttccac aaccataaaa cctgtctatt acatcctgtg ggatatggct tgtgtcatcc
     1261 aagggataag atgttggaca gcatagatct gcctgaggtt gctcagcagc tttaccataa
     1321 aaatctctta cagattccct tgatttatca aaatcatagg tgagtacaca gtttgaatgc
     1381 gatacttcga ttattagatc tgtgtagtta tgtattccat tgggatggcc atttgatgat
     1441 aagccgtttc catttgtatt tccattgccg ttaatgttgc agtccagtac ttcttctccc
     1501 atggatttgt aaacaatggg tgaatcaaat ccagaggccc tgttaaattt ccgggcactt
     1561 tcttgtgcaa gctcatatag tgaagtatta atgatatatt catgcagctc agagtatgga
     1621 tcgagcccct caagatcacc tttaccaaaa gtggaagctg tatagaaata tgtatgctca
     1681 atatctcctc caccgcatat atattttata tagcaattat tgcattttgt ctttttttgt
     1741 accgtagcag ttctaaactt attaaaaacc ggactctcct tccatatgga ttcaagagtg
     1801 ttatctaaaa tactgcctcc gctaagaccc gggtaatttg caaatgctgc cgatggataa
     1861 accttcccgt ctgaatatac acacaggctt ttccagcatg cattgcttag gtcatgttta
     1921 gtaaactttt tggatttaag tttagtcttt atgctttcaa agttgtctat tttaattcca
     1981 agctcatctg caactgattt tgtttctcta agtacttcta tcagtttgta tgtaggtact
     2041 atcatgctgt cattttgaga gattcttcct ctttgatgca cccacagaag atgatgatta
     2101 tttactccta aaccatgaat aagtcttgtg atattactta tgtcatgcag gttatcagaa
     2161 ttaacaaccg ttgttacagt tggatcaaga ccacttttca ctgctgattt aattccttca
     2221 atgatttttt taaatgttcc cttaccccta atagaatcgt tggcttttgc agtcgagccg
     2281 tcaaggctta tctgtggctt aaccctgccg cggcttactt tttgaaactg ttttaaatta
     2341 tctttatgat taaagaacat tccatttgta agtataatca gttctgtata aggggcggtt
     2401 gtgatgtaat caattagttc aaatatgtct tttctaataa acggctctcc accagtaaaa
     2461 tagaatctat acacaccaag cttttttgcc tgatcaatga ggtctttaag aactgaggtt
     2521 tccagtcccc tatctcctga tgggcctgat tcaaccaggc agtgggtaca ctgaaggttg
     2581 caagaattat tcgtatgtat ccatagttca ctaaggtctg taagctgcaa ataatctgat
     2641 cttcctttat atggcgctct agagtagggg gttaatgata caaatttctc tcttacgcta
     2701 tctttaatga atgcatcaac atctacccat gcttttgcaa atgggatatt ttgttctgta
     2761 gaataaagtc ttaccacttc atcaaaagtt tttttcccgt caatgatgct taatattttt
     2821 gctcctttgt tgtcagtgct aatccagtta ggaccttccg ggtcaataaa aatctggatt
     2881 ctgttgtttg aatttaactt tgaataggat ggagcatgaa gaatttcgtt tgataggttg
     2941 tccattaaat ggtctcctgt gattgtgaag atagattgat ataataataa ttatattaat
     3001 cttattgatt tatattatta atttctatac tttgatacaa aagatcataa aaaagtttca
     3061 aaaatgagtt taaccataat ttacagagat acaaaatgat aaaagcggtg attttcgatt
     3121 ttgacggagt tatagctgat acagaacatc ttcattttga agtgttccga aatgtattga
     3181 atcatcaaaa acaagatcta acagaaaatg aatattattc acaatatctg gcatatgacg
     3241 ataaaacatt tttttctaaa tattatgaaa ataacgaact taaattggat aagaaactgc
     3301 ttaagagact tctgaagaac aaatctgaag agtttgatag tctcataaag aataatataa
     3361 agatatatcc cggagtagtg gattttattg agaagctttc tgtgaggtat gatttagcta
     3421 ttggctcagg cgcgctcaaa aaggaaatag tccagatact ggatgttata aacattgctc
     3481 atttgtttaa aataatcatt tcggctgacg aaatatcaaa ctgcaaacca aatcctgagg
     3541 tttataacac agtgctttca aggttaaata atataaaagg taatgatata agcgctgatg
     3601 aatgtattgt aatagaagat tcaatttatg gtattcaggc tgcaaaaagt gcgggaatga
     3661 agtgtatggc tgttacaaat tcataccctg ctaaaaagct tacagaagca gatttggtag
     3721 tatcagatta ttcggtaagt gatattgagc agatctccgg ataaattgta tagcactagt
     3781 taataattag ttaagattaa cagaacattt aaaactaact ttagttttaa gcttagatct
     3841 aaaattgccc aagaataatg tttgctattt agtgttgatt attaacatta actattgatt
     3901 ctaatatact taaatcatag ccttaatatc taaatattat ttaaaatata taaatatttc
     3961 aagaatttat atcaatattc caacaataca ataattatcc aatagttcta ggttgttaca
     4021 aaaatataaa ccatatttta tacctttgtc ctattttaat atattaatct ggagttataa
     4081 tatctaaaag aacaaaattc tttatttcat catcctccct tgggcaagct acttaatgcc
     4141 agttaagttg cttgcccttt tttatatacc aatatttaaa ctaatttagt tgataattgt
     4201 tatataaatt ttttcttatt tttagtactg tatcaattac caaaatcgct attgactttt
     4261 tttgataaac gtaataagct taaagtaaat cctaaacgaa atggcaaacc atttgaaaag
     4321 atgggacgca aagccgacgg cctaaagatt atataatcta tggcggcagg gttaccgaag
     4381 taggtacttt tacattccta ctttttaata tcctttattg gcttgtttct cttgttttca
     4441 aataattcta gtattttgcc caagtgagga tatttgatga aatatatttt ttgggaggtg
     4501 atgattactt aagataattt aaattgttaa atttaatatc ttaagtttca cacaataaaa
     4561 tgtcctaaac gataaaggca aaccatttga aagggtggga cgcaaagcca acggcctaat
     4621 gactgtgtag ttactggcat atatacagtc tacggcggca gggttaccga aaattaggag
     4681 aacagaaccc tctaccatct tctctctcct ctattttcct tcctttcttt aacaatctgt
     4741 tttttatcat aggacatgta cttgcccggg gcggatgatg aaaaatattt gtataaggag
     4801 gtgatattaa acaacatata aaaagaatat tcaaaaagct cctaaacgac aaaggcaaac
     4861 cttttgaaaa agagggacgc aaagccaacg gcctaatgac tgtgtagcta aagcataaac
     4921 ataatctatg gcggcagggt taccgaattt tggatgacaa atagtatatg aatcacaacg
     4981 ttcatattcc tctggatgtt tagggcttaa tgttatttgt ttcaaataat gtttaaaaac
     5041 tagattgata gggggtattt atgaatacat taacagtttt aacattatta gttctagctg
     5101 cggcatttat gtttgcagct tttgcttcaa taatttatca agtgtagttc tcctttatta
     5161 catttgataa aagtataaaa ataaaaagat aaagatgtga tcgggatcac atttaatatc
     5221 taaaaaaaat taaatcttgg gaggatttaa aatgtataag atttttacaa cttttttatg
     5281 ttcaattttt gcatgtttaa tctttatttc agttaattcc gtaaaggcag acgactgtgt
     5341 agctcctgta tctatgagct gtagttacga agaaacagga gacactacta tagtggcaac
     5401 agtgacagtt agttatctgg ttgatggaag tacagcttca gatttatcct cagttgtttt
     5461 ttctgataac tgtgatgcaa ccgcagttgg cgtaggtgtt gaagtagtgg accctacaca
     5521 cgacacaaac gcagatgata ctaatccatg tgcagaaggt gatatgaaaa tcactgtaga
     5581 tgattatgat gatttttgca ctcttgctac ttctgcaaca tgttcagctg gaaatatagg
     5641 tgctatttgt agtgcagata cggattgtga taccagtgac gcatcaggtg acggagtttg
     5701 tggtgatggt atttgtgatg caacaagcac aacagctaca tgttcagccg gtcttgtagg
     5761 gattgcctgt tcagcagatg tagattgtga tgacataggt ggtgatggtg tttgtggaga
     5821 ttcttcttgt gtagcaatgg tttgtgtagg aggtataaat gatacgcaag catgtgcatc
     5881 tgacgttgat tgtcctgata cattgagctc cacttgtgaa ggcgggtcag acgacacact
     5941 taaaatctgt acagtaaatg atgactgtgc agacactcag actgcagcgt ctattgaatg
     6001 tataaaaaca attgatgaaa tcgatattag tgcatgtgat gaggaaattg caactatagc
     6061 agaaagctct aaccttcatg accgtcaggt tgaagatagt aaagactgta atggcagaag
     6121 atgtaaaaat gacgatgatg ataaaggtca tgacagaggt acggtattgt gtacagtacc
     6181 agaagtatga tctgattaat ttaatttgaa acaatattta tataaaaagc cgggtaatat
     6241 ttttatttgc ccggcttttt tattttatct ttggtaatac aaaataattt gttatagttc
     6301 atatttaatg gaccgaagaa actttataaa atacatctca tctacagcac ttttaagctt
     6361 tattgtccct attaacctat cttttggaca atcaacttat aaagaccggg taatttcaaa
     6421 gtttattgat ttaattcttc ccctaaataa ttctgccatt ctcaatgtag aatcattcag
     6481 gtccaagctt gatcaaaatc ttagaaataa taaaaatgct gaaaagatta ttgattatgg
     6541 aattaaattc tttgaccata atgcaaacct tctttatcaa aaaccccagt ttttgcagct
     6601 ttccaaaact gagcagctgg aaataattaa ctttacgata aataacactg tttctttaga
     6661 caagcccgat ccatccaaac cctggactga tataaggtac ggcaaaatgt tttttaataa
     6721 tcttagaagc tttgtgatga atgaattcta ttcaagccct gagggttgga agtttattgg
     6781 atacaaaggt cctccacagt ttggtggaaa tcttgattat actaaatgta cttattaaat
     6841 ggctaaacaa actgaatatg atgtctgtat tataggttct ggtgcgggcg gcgctcctac
     6901 agcttatgcg ctgggaaaag ccggtttcaa aactatcgtt ttagaagcag gcagaagatt
     6961 taaaccagaa gagtatcatg taaataaaaa ggactgggaa aattacccat atcctcagcc
     7021 gtttacagat tttaagaata gatcaaaaga aagatatact tcagcaccaa ctgaaaaact
     7081 aaatcttaaa tattctcatt taagatcaaa ggcaaagtac agggggcagt acaacgggtc
     7141 taacaacaga caaagaccat ttgtacagag ggcaaaaggg atagggggca ctacacttca
     7201 ctaccaggca gaagcgcaaa ggttctcaga ttatggattc aaaacaaata gtctgtttgg
     7261 atttggagaa gactggccta taacttataa agacttagaa ccttattatg ataaagcaga
     7321 aaagatttta aatgtttcgg gacagcttgg aaatcctgaa aaaccaaaac gcattccctt
     7381 cccgcaacct gcacacgaac ttagctgtgc tagtaaaaga gtaaaagtgg gttttgataa
     7441 actggggtta agtcttctgc ctaatacact gaatatatta tccaaacctg ctgatggcag
     7501 gcccgcatgc aattactgca acggatgttt tcttggatgt atgatgaggg ctaagggcag
     7561 tatggatgtc acatttatac ctaaagcaga ggctactggc aatgtaactg tacaatccaa
     7621 ttcagcagtg agagagatta cggttaatgc taaaggtaag gcagacggag ttatatatct
     7681 tgatgactct aaaaccgagc acaggataaa atcaaaaata gtagtagtat caggcggagc
     7741 attagaaacc ccgaggcttc ttctaaattc taaatcttca cagtttccag acggtctcgg
     7801 aaatagctca ggccttgtgg gtaaaaactt catggagact gttgtatata taaacacagc
     7861 tatttacgaa gaagatattg agtcctataa gggtcttcaa atagacagca ggagttatga
     7921 ctataacaag acttctgcag acaggggatt taacggcgga gtagtatttg gactgtctgc
     7981 tatggacctt cttggacctc tgggatatgc aagaacaata gcgcccacat ggggagatga
     8041 gcatgtgaag tttatgaatg actatttcgg ccatgcaata aacatttttg caatcggcga
     8101 gatgattccc cgtgagacaa atactattac aattgattca tcaaagactg attattacgg
     8161 aatacctgta gccaagatca agaccagaat gagtacaaat gatctggata tgatttcttt
     8221 tatgcagaag aagacaagag aaattataga ttcatccgga gcaaaagagt ctgttagaga
     8281 gttgagctct tatgatctaa gtttaatttc tcatattggc ggtactacaa aaatgggaac
     8341 cgatcccaaa aaatcagtac taaacgcgtt ctcacaaagc catgatgtta agaacctgtt
     8401 tgtctgcgat tcaagcccct ttgtatcgca gggtggtgga gacagcccgt ctcttactat
     8461 ccatgcactt gcactgagag cctctgatta cattatttct gaggccaaga aagggaatat
     8521 ttaattcaat aaaattctct tgacttagta atactacagt attaatatct aaattagatt
     8581 attatatata tccaagagga tgaaaaattg aaaaaactac tagtagtacc tctattgttt
     8641 tttgttttag gaatacagaa ttcatttgcc gggggagcac caactccaac tccaatacca
     8701 gtccctgaag gtgttgcttg tttgcagcct acagttgatg gatgtgcttg gtttcctgat
     8761 gcgttaatgc ccggagtaac tggagacgat gatgattcgg gtccgtcaat ttcaggttat
     8821 ggatatgtat cggttcctgt tgttagtgat agcaatgcaa aatgtgtagg tgatgatgat
     8881 gattgtaatg gaggtatcaa tgagggtgaa gcatgtaatg atgatatgga ctgtccaata
     8941 gttggttgta tcaattaccc agagtgtgct actaataaac ttgcatggga agttgatgtt
     9001 cacgatactc attcttcatt ctatgaccca aacccttgtg cttctacagg tgctaaacta
     9061 gattttcagt gttctgacaa cattacatca atacctgagg gttgtaattt tattcaggct
     9121 ccacagccag gtcttatagg cgttgatgaa atcagatgcc cggtagagtt tgatgggctt
     9181 agcgtaggtt attgtgattt atacaatgat gatgatgatc taataggacc tgtgtttata
     9241 gatggaccac cgccaatagg ggttaaactt agtggtaagc atttaaatca gcaggctagt
     9301 gaaggtagac aaagaggtgg aaagaataca tcaagaaatg ggacaaacca gactggtgtt
     9361 gcccattgta taccgcggtt tataatagaa aactaaaaaa ttaataataa aagaattaaa
     9421 aaagccggac ttagttccgg cttttttgtt tgtcctgttc c
//
LOCUS       DBNGSV010000083        15490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1678265, whole genome shotgun sequence.
ACCESSION   DBNGSV010000083 DBNGSV010000000
VERSION     DBNGSV010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15490
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1678265"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..2511)
                     /locus_tag="AC159U_02930"
     CDS             complement(187..2511)
                     /locus_tag="AC159U_02930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3336043.1"
                     /translation="MNIKNDLDFITKESLITYGQTMAEASRAEGAKPPSKPKASINRI
                     KKVQSIKDEIKQFVVRAQKGFETADDYIKQRQLIIENSCSGDALLFYTAWNRMLIEGE
                     LNALLSSPIGSIQKPIRRRPTSIVPRDQLSPGLTEGRIVIDLGDDRYWLVPKELADRC
                     LLFTLRHGLSKVDSSTYRVGRRLANTLDEDKGVLKAEAVGKALARMLGIVGQQLEFLD
                     VPNFLDPKKFKHKTSSSPNTRQLYDHIIKSILDKDTKRKKPQFADALESQDFGWVTGV
                     EKKFEISEAARIFGCDEKTAKKLIKNPLYCYPGGQSFFDLYVEVTGGFRKLALNNKGN
                     VLCLYTHSSTLRALIIFLDPRPFAEAFEEFGEYKEKQDNVVLLALEQGYLTGYSTAVG
                     LSAAEQQTRETWKSLEEKRKSRIKLKPGKIKKIVALVSGGDFSGAGAALKELRVVGKR
                     FGIEVYFVRHGFLGLANNWIETVSEEDTRGMVSMPSTPIGSSRFEEFKDERVQDIAYK
                     NLQPYIKNGALVVIGGDGTLRGGRALFERLGVHVVAIPGSIDDNIEGTTSLGFHSAVA
                     LANQSLESLKATSSAMGSIFFVEIMGAGSGHLALACGFQARAEGVLINEHPDPDAYID
                     RVILGTLKRTLGVPNKSHLFIVAEKTPHRHHELGGIHGLSEYVSEQISKWDNIESKKG
                     LYNLPVATKATILGHTLRGAPPSHVDKGLGQYLAYETVKRLVEDPMSIVGCCLGTHGL
                     GHVESIPLHAVKPKQFDWQLFTRMHGIEYISDTN"
     gene            2847..4331
                     /locus_tag="AC159U_02935"
     CDS             2847..4331
                     /locus_tag="AC159U_02935"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3336044.1"
                     /translation="MIGKTIGPYNITGVLGKGGMGIVYKAIHTTLEQQVAIKALSSDM
                     SDNPSNRQRFIKEAKIHAKFSHPNVVNIFNYLEEENDVFIVMEYVDGETLDQKLKREG
                     TISEEQAISLSLPVLQALDFMHSNGVVHRDIKPSNIMITKTGLVKVTDFGIAKIVNEK
                     TQQTKTGMVGSLYYISPEQILGEQTSAATDVYSFGATLFHMVTGKALYEGTEYAIMRS
                     HLEGKPIPPWELNDSVSKNFGKVVLKAIQKAPRDRYKSAKEFSSDLIKVQSGSQISIS
                     LPGILKNNKNRLTEVVSKRKRAIYGGIAAVLLFTVIAFGINRDPQQQNVNPVNTSSVI
                     DKGSKEQLLPNTEIDTVTDINQTKSVPKSLTSTNIKPSQRKPIKTQKQFEEDERFILF
                     DEELYKEKNTRKSAEQNKKLEEKNRILEDKLREEQKRYEAKAREKRKREQRLKREEEK
                     KLREQEKKQNGGVKNSYKKVYNPVKKNVFHPIKKGFNKLKDKID"
     gene            4385..4933
                     /locus_tag="AC159U_02940"
     CDS             4385..4933
                     /locus_tag="AC159U_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336045.1"
                     /translation="MNHPEILLLPIMMFADYFLTILGAIKIEQGYSKHFKTEHYELNP
                     QWQQDVKEIKWFNIKHIIVTILVTIGLGYIFESFYLPYALIKMLIGCILVLYAMIIGR
                     HITNLLIFSYVNRNPGHISGEVNMTHEFVLYLSLFQTVMVLVPIILIAIFSNSYFSYG
                     GAIGITLFILVNYQWIRKANRQ"
     gene            5167..5451
                     /locus_tag="AC159U_02945"
     CDS             5167..5451
                     /locus_tag="AC159U_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336046.1"
                     /translation="MEKMMGNMDKGEMKKMMEQMMSKCCGDMSEEHKEKAAQEIKTGC
                     CEEGEMPQMPQMIMKMMPHCLEMILPKLSEEDRNKFVMEMASILFAKQKP"
     gene            5734..5907
                     /locus_tag="AC159U_02950"
     CDS             5734..5907
                     /locus_tag="AC159U_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3336047.1"
                     /translation="MSCWWGHRMMDTPEIRDAIKRYMGEHSESALEILNKRYAKGEID
                     KQEYEEKKAAITG"
     gene            6017..6439
                     /locus_tag="AC159U_02955"
     CDS             6017..6439
                     /locus_tag="AC159U_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3336048.1"
                     /translation="MNVKVADLMIKNVINCGPHNSIEHVRNIMKKNKVHAIPIVDDDE
                     ILKGIVSTLDLTDSLKDNTPISKIMTEKVYSIPLYNDIHHAARLMRNHHVHHVVVTNE
                     KKVVGILSSFDLLKLVEKHRFEIKPAPSKSTKKKARRE"
     gene            complement(6574..7251)
                     /locus_tag="AC159U_02960"
     CDS             complement(6574..7251)
                     /locus_tag="AC159U_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3336049.1"
                     /translation="MSENYDEVNKYISKFENLVPELNETWGGFRDEVFKEGLISAKDK
                     QLIALACAYITESAHDIRERTIKSKELGATDEQIAESVYIAMRLAIGQPYAFSSIALE
                     NYDLMKNKESVVKGYFISKNITPQIQDFHKVSGSMHEKFNAFHEIVYEDGHLPKKLKK
                     GLMGLACAILAKCPWCIRSCVRDGLQEGVVKEEIVEAINIAMVMNASACVANTGVSMQ
                     AAEEISS"
     gene            complement(7305..8324)
                     /locus_tag="AC159U_02965"
     CDS             complement(7305..8324)
                     /locus_tag="AC159U_02965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3336050.1"
                     /translation="MKAYQISKQDKLYEMNLNEIPEPEIGPNDVLIDMKAWSLNYRDL
                     SMLRGGYYRNDKVKTNPPLIPLSDGAGEVVSVGNSVTRFKKGDRVAGIFFQTWISGDL
                     TDEQIGSALGGAIDGVLAEKVVLNENGVVKIPDNLNYHEAATLPCAAVTAWQALNFNP
                     VKPDDTVLMLGTGGVSIFALQFVKAEGAKAIITSSSDEKLEMARKLGADHTINYKEHP
                     DWEKEVLKITDGQGVDNVIEVGGAGTLEKSITSAKVSGRISLIGVLSGQPEQNPSPVM
                     IMLKRLTLQGIYVGSRDMFESMNKVIEANDIKPVIGKTFEFENATESFDYLKSGSHFG
                     KIVIS"
     gene            8422..8784
                     /locus_tag="AC159U_02970"
     CDS             8422..8784
                     /locus_tag="AC159U_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015465148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3336051.1"
                     /translation="MRTEADTTEDKKQVADFSDPCILPCPIERGMRIIGGKWKGSILW
                     YLKDGPIRFNELARKLKGASKKMVNQRLKEMEDIGLLKRRVISTKPIAVEYEITDFGR
                     SALVFLNELKDWSLENNI"
     gene            9217..9441
                     /locus_tag="AC159U_02975"
     CDS             9217..9441
                     /locus_tag="AC159U_02975"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336052.1"
                     /translation="MSKESENISDITVLDMGAGNGIVAEELVNLGLDDVIGVDIIEEA
                     KQAAFRDRPEVYSDYIFDDLTLPDQTTQRF"
     gene            9471..9746
                     /locus_tag="AC159U_02980"
     CDS             9471..9746
                     /locus_tag="AC159U_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336053.1"
                     /translation="MAAIGYGDIPPKTLANAINYTDEQGWVALCVKEDFLKEDDSYGV
                     CELYEELIQQDVMDIKKKKNYMHRKSISGERLMYDAIITKKNTDIPQ"
     gene            10051..11142
                     /locus_tag="AC159U_02985"
     CDS             10051..11142
                     /locus_tag="AC159U_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3336054.1"
                     /translation="MVFTELIKNTYLGNSLQNWLIAITVAIVLSLILNSINKYLFLKL
                     SKHAENTSTKLDDILVGFLSKTNFMLVIVFSIFVGSLFLDLNKTLLVIRKSVIIIAFL
                     VQTALWGSGVITFIVNRKLNKYSFETSRTAITQIKSMGLVSKIVLWVVIFLLILDNLG
                     FNITTLIAGLGIGGIAVALALQNVLGDLIASLSIIVDKPFVVGDFIILDDFLGNVEHI
                     GLKTTRVRSLSGEQIIFSNNDLLSSRLRNYKRMYERRIVFTIGVIYQTTAEMIEAIPK
                     WIKEIVDSEPNARFDRSHFSSYGDFSLNFETVYFVESPDYNVYMDIQQNINMGIFRKF
                     EQERVEFAYPTQTLFLARNVSNSELSNNS"
     gene            11472..12125
                     /locus_tag="AC159U_02990"
     CDS             11472..12125
                     /locus_tag="AC159U_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3336055.1"
                     /translation="MDILQILANTDDGVFAVGTDHKIVFWNKPAQKILGYSANDVLGK
                     YCNEIIAGTDNSGNPVCSKSCNVMKAIKKEENPQNYDLITHSKKGDPIWLNVSIICIP
                     GSQPALSTVVHIFHHITKQKLEFHRIREAVSEINNESKLENKNLSKNQSKTQGTLTPR
                     ERKVLKYLEEGCRPVDISEKLYISLSTLRKHIQNIHTKLEVHSMLEAVAKARQNNLI"
     gene            12386..13081
                     /locus_tag="AC159U_02995"
     CDS             12386..13081
                     /locus_tag="AC159U_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336056.1"
                     /translation="MRKIIYTLLFLNIAVLIGYHALYAASASIGNHSCIGPDACTSNT
                     GDIGNNSCNGEEACDENEGDVGNKSCHGLEACADNNGDVGNNSCNSQDACQVNDGSVG
                     HNSCNGTDACGSNGGDIGNRSCNDDFACTSNDADVGDNSCRSMDACDRAGRDGMGDTI
                     GNYSCIGYKSCEDNDASSTGINSCHGEHACDDVTDGITIGNNSCNGDRACACRTTNVP
                     DNTCNGEEACFEC"
     gene            13526..14461
                     /locus_tag="AC159U_03000"
     CDS             13526..14461
                     /locus_tag="AC159U_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3336057.1"
                     /translation="MVKVAIVQESPAFLNKVKTIKKAVALIKQAANSGAQLIVFPEAF
                     IPAYPAWIWRLRPGGDWNISEKLYASLLDNSVNLDSDDISPICKVAKKHKITVVCGLN
                     EKDSKFSQATLYNTVIIIGSDGAILNRHRKLMPTNPERMIWGFGDGSGLKVVDTTIGK
                     ISTLLCWENYMPLARYALYSQGVEIYIAPTYDSGDGWIGTLQHIAREGRCWVVCSGVA
                     FENSDIPPDFPEKKALYPDSEDWINPGDSVVIKPGGEIVAGPMHKEKGILYADLDIKL
                     VGTAKRTLDVAGHYSRPDIFKLSVNTNPQSPVKFE"
     gene            14493..14927
                     /locus_tag="AC159U_03005"
     CDS             14493..14927
                     /locus_tag="AC159U_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3336058.1"
                     /translation="MSKEKTYTGNCFCGEVEFTVTGEPAVMGYCHCDSCRHWSAGPVN
                     AFTLWIPEAVEVIKGKDNIDTYNKTPQSYRKWCKTCGGHIFTEHPGMGFIDVYAVLIQ
                     DFPFKPGFHVNYQETVLHIKDGLPKLRDLPKEAGGSGEVLPE"
BASE COUNT         5033 a         2688 c         2979 g         4790 t
ORIGIN      
        1 aaacactttg ttttgtatct aaaagagatg gaatttagat ttaataacag aaatgtagat
       61 ttagttaata aaataaagga gattctgcat tacttatagc tctgttctat gcgagctcct
      121 taatttatat tccagccagc ctctatggtt ggctggcgtt ttatattcac taaatactaa
      181 ttcagattaa tttgtatccg atatatattc aatgccgtgc attctcgtaa atagctgcca
      241 gtcaaactgc tttggtttta ctgcatgaag cggtatggat tcaacgtgcc ctagtccatg
      301 ggttccaaga cagcagccca caatcgacat tggatcttca acaagacgct taaccgtttc
      361 ataggcaaga tactggccta aacccttgtc tacatgagac ggcggggcgc ccctcagtgt
      421 atgtcccaga atagtagctt tcgttgctac ggggagatta tataagccct ttttgctttc
      481 aatattatcc cacttagata tttgttccga tacatattct gaaagaccgt gtattcctcc
      541 aagctcatga tgccggtgag gggttttttc tgctactatg aacaggtggc ttttgtttgg
      601 aacgccgagt gtgcgcttga gtgtgcccag tatgacacgg tcgatatatg catcggggtc
      661 aggatgctca tttattagca ctccctctgc ccgtgcctga aatccgcatg caagcgccag
      721 atgcccagag cctgcaccca tgatctctac gaaaaatatg cttcccatag ctgaacttgt
      781 agcctttagg gattccagtg attggtttgc aagtgcaact gcagagtgaa acccgagaga
      841 agttgtgcct tctatattgt catcaatcga ccccggtatg gctacaacat gtactcccag
      901 tctttcaaaa agggctctgc ctccccttag cgttccatca ccgcctataa ctaccaatgc
      961 accgttttta atgtatggct gaagattttt gtaagcaatg tcctggaccc gctcgtcctt
     1021 gaactcttca aacctgctgc ttccaatagg ggtgctgggc atacttacca ttccccttgt
     1081 atcctcttct gaaacggtct ctatccagtt attggcaagt ccaaggaacc cgtgccttac
     1141 aaaatatact tcaattccga atcgtttccc tacaactctc agctccttta gggctgcgcc
     1201 tgcgcctgaa aaatctccac ctgatacaag tgcaacaatt ttttttatct tgccaggttt
     1261 taacttaatt ctgctttttc ttttctcttc taaagacttc catgtctcac gggtctgttg
     1321 ttcggcagcg gatagtccaa cagcggttga ataacctgta agatagccct gctcgagcgc
     1381 cagcagtacc acattgtcct gcttttcttt atattctcca aactcttcga aagcttcagc
     1441 aaatgggcgg gggtctaaaa agataatcag tgctctcaat gtggagctat gtgtatagag
     1501 gcagagcaca tttcctttat tatttaaggc aagttttcta aagcctcccg taacttctac
     1561 atacagatca aaaaaactct gcccgccggg atagcaatac agcggatttt taataagctt
     1621 cttagcagtt ttttcatcac aaccgaaaat ccttgctgct tcgctaatct cgaatttctt
     1681 ttctacacct gtcacccagc caaaatcctg agattctaaa gcgtcagcaa attgaggttt
     1741 tttccttttg gtatctttgt ccagaattga ttttattata tgatcatata gctgtctggt
     1801 gttagggctt gagcttgttt tgtgtttgaa ttttttgggg tctaaaaaat ttgggacatc
     1861 taaaaactca agctgctgtc caactattcc aagcatcctg gcaagcgcct ttcctacagc
     1921 ttctgccttt aatactcctt tatcttcatc aagggtattc gccagtcttc ttcctactct
     1981 gtatgtgctg ctgtcaactt ttgaaagccc gtgtcttaat gtaaagagta aacatctgtc
     2041 agcaagctct tttggtacaa gccagtatct gtcgtctcca aggtctatga ctatacgacc
     2101 ttctgtaagc ccggggctta actgatcacg cggaacaatc gaggtcggac gtcttcttat
     2161 tggtttttga attgagccta ttgggcttga aagcagggcg tttaattcac cttcaataag
     2221 catgcggttc caggctgtat aaaagagcag ggcatcaccg ctgcatgaat tttcaataat
     2281 aagctgtctt tgctttatat aatcatcagc tgtttcaaac cccttttgag cacgtacgac
     2341 aaactgtttt atctcatctt ttatagattg tacttttttg atgcgattta ttgaggcttt
     2401 tggttttgat ggcggctttg ctccctctgc ccttgaagct tcggccatag tctgtccgta
     2461 tgtaattagc gattctttag ttataaagtc cagatcgttc ttaatattca tttttacccc
     2521 atttataaaa ttcaactcta gcacagaatt tatacttgcc aaattcataa aatatatatg
     2581 atgtttcggt ataatttata aatcaagaat aattgaaata tttctgtagt tgaatgatct
     2641 gattatttgt tgttcattag gattcgtatt gaaataaata ttattaattt acattctatt
     2701 acagaattgt gttttaaaat gttaaaatat acaggtggta caaaacgcta aggggtaaat
     2761 tgaggagcag ttagtaaaag ggaataggtg atgaaaaatt gtttattaca actgctgtcc
     2821 tatctgtcat ctactgagat tgaaaaatga taggtaaaac aatcgggcct tataatatta
     2881 caggggtttt aggaaaaggt ggtatgggta ttgtttataa agccattcat actaccttag
     2941 agcaacaggt tgccattaaa gcactgtcgt ccgatatgtc agacaaccct tccaacaggc
     3001 aaaggtttat taaagaagca aagatacatg caaaattttc tcatccaaat gttgtaaata
     3061 tttttaatta tctcgaagaa gaaaatgatg tatttatcgt tatggagtat gtggatggtg
     3121 aaacactgga tcaaaagcta aaaagagagg gaacgatatc tgaagagcag gcaatttcat
     3181 tgtcccttcc tgttttgcaa gctcttgatt ttatgcattc aaacggagta gtgcacagag
     3241 acataaagcc aagcaacatc atgataacaa agacagggct tgtgaaagta actgattttg
     3301 gtatagcaaa aatagttaat gaaaaaaccc agcagacaaa aacagggatg gttggatcac
     3361 tttactatat ttcgccggag cagattctgg gtgaacaaac aagcgctgca acagatgtat
     3421 attcatttgg tgctacgctc tttcatatgg ttacgggtaa ggctctttat gaaggtaccg
     3481 aatatgctat tatgagaagc catctggaag gcaagcccat accaccctgg gagttaaatg
     3541 attctgtatc gaaaaatttt ggaaaggttg tgcttaaagc tattcaaaag gcaccccgag
     3601 acagatacaa aagtgctaaa gaattttctt ctgaccttat aaaagtgcag agcggctctc
     3661 aaatctccat atccctgccc ggaattttaa aaaacaataa aaaccgacta accgaggtcg
     3721 tgtctaaaag aaaaagggca atatatggag gaattgcagc agtattgcta tttaccgtaa
     3781 ttgcttttgg tattaataga gaccctcaac aacaaaatgt aaatcctgtt aacaccagct
     3841 ctgtcataga taaaggatct aaagaacaac tgttacctaa cactgagata gatacagtca
     3901 ctgatataaa tcagactaaa tctgtaccta aaagtctgac atcaactaat attaaaccat
     3961 ctcagagaaa acctataaag acacaaaaac aatttgaaga ggatgaaaga tttattttat
     4021 ttgatgaaga attgtataaa gaaaaaaaca ccagaaaatc agcagagcag aataaaaagc
     4081 ttgaagaaaa aaacagaata cttgaggata agttaagaga agaacaaaag aggtatgaag
     4141 caaaagcccg tgaaaaaaga aaaagagaac aacgactgaa acgcgaggaa gaaaaaaagc
     4201 ttagagaaca agagaaaaaa caaaatggcg gagttaaaaa tagctataaa aaagtatata
     4261 atccggttaa gaaaaatgta tttcatccaa taaaaaaagg gtttaataag ctaaaggata
     4321 aaattgattg atactgttct caaaagatat atatagctat tactaattta aaataatatc
     4381 gattatgaat caccctgaaa tattactcct gccaataatg atgtttgccg attatttttt
     4441 aaccatcctg ggtgcaataa aaattgagca aggatactcc aaacatttta agactgagca
     4501 ctatgagctt aatccgcagt ggcagcagga tgtaaaagaa ataaagtggt ttaatataaa
     4561 acacattatt gttacgatac ttgttacaat tggactaggt tacatttttg aatcttttta
     4621 tttaccttat gctttaatta aaatgctaat aggctgtatt ctcgttctct atgcaatgat
     4681 cattggaagg catattacca accttctaat cttctcgtat gtaaatcgaa atcccggaca
     4741 tatttcaggt gaagtgaata tgactcacga atttgttctt tacctctcac tgtttcaaac
     4801 tgttatggtg ttagttccaa ttattctcat tgcaattttt tcaaatagct acttttctta
     4861 cggcggagca attggtatta ccctttttat tcttgtgaat tatcagtgga ttagaaaggc
     4921 taatagacag tagttttatt tatttcagtg cttttatata tgcgggtgat actttttttg
     4981 tttaaggaag tattagtatt tttcaataat gtaatataat aggacatatt gcatcttttt
     5041 aaatatttaa gttatataac accttgtaaa cattaactat tttgtggctt ataatggcat
     5101 aatatatgca atttattcta ttaaaacttt attttggggg taagaaaaat ggaaatgatg
     5161 gaaaaaatgg aaaagatgat gggaaacatg gacaagggag aaatgaaaaa aatgatggag
     5221 cagatgatgt caaaatgctg tggggatatg tcagaagagc ataaggaaaa agctgcacaa
     5281 gaaattaaga ctggctgctg cgaagaaggg gagatgcctc agatgccaca gatgatcatg
     5341 aagatgatgc ctcactgcct tgagatgata cttcctaaat tgtcggaaga agacaggaat
     5401 aaatttgtta tggaaatggc ttctatacta tttgcaaaac aaaaacctta ggtaaatatc
     5461 tgtctaggtc ggaaaatatt aattgtggca aataataata aggagaaata tgtgagtgca
     5521 ataaaatatg ctctgatatc tctcagtttc gtttttctca atctagtatt tgtctgcccg
     5581 gtattcggtg cagaacagcc tccgccacat acacatccat acccatggac atcgtggcat
     5641 ggaatgcagt ggcccgcata ttggtggatt ttccccctac tgtttttcat tgtgatgtgc
     5701 gtgctattta ttttcttcat gaaaagaggt ggcatgagct gctggtgggg tcacaggatg
     5761 atggacacac cagaaatccg tgacgcaata aaaaggtata tgggtgagca ttctgagtcg
     5821 gctcttgaaa tactaaacaa gagatacgcc aagggcgaga ttgacaaaca ggaatacgag
     5881 gagaaaaaag ctgcgattac aggttaatag taatagcttt tgttaaaagt atatagtcag
     5941 tgggttcagt ataattataa aaaactatta atacagtaat aactaaaaag actatagtta
     6001 gagtggagga taactcatga atgtcaaagt agccgattta atgattaaga atgtaataaa
     6061 ctgcggacct cataactcaa ttgaacatgt aagaaatatc atgaaaaaga acaaagtgca
     6121 tgcaatcccg attgttgacg acgacgaaat cttaaaaggc attgtatcta ccctcgatct
     6181 tacagacagc ctcaaggata atacaccgat cagcaaaatt atgaccgaaa aagtttattc
     6241 tattcctttg tacaatgata ttcatcatgc tgcaaggctt atgcgaaatc atcatgtgca
     6301 ccatgtagtt gttacgaatg aaaaaaaggt agtaggaata ctaagctctt ttgatctatt
     6361 aaaactggta gaaaaacaca gattcgagat caagccggcg cctagtaaat caaccaagaa
     6421 aaaagctaga agagagtaaa taaagtaccg aggctacagc aatatttagc tgtgttaacc
     6481 tggatttttg tatacgctta gaactcgtgt tttgtaataa taatggaaag agttacttat
     6541 tggccgattt aaaaattaaa tcggcctttt tcattaactg ctaatctctt cagctgcctg
     6601 catggatacg ccggtattag caacacaggc gctggcattc attaccatag caatattgat
     6661 tgcttcaacg atttcttctt tgacaacacc ttcctgcagt ccgtctctta cgcagcttct
     6721 aatacaccac gggcatttag ccaatattgc acaggcaaga cccataagtc ctttttttag
     6781 ctttttggga aggtgaccgt cttcgtatac tatttcatga aatgcattga acttttcgtg
     6841 cattgagccg cttactttat gaaagtcctg tatttgggga gttatatttt tgcttataaa
     6901 gtaaccctta acaacacttt ccttgttttt cattaaatca taattttcaa gcgcaatact
     6961 gctaaatgca taaggctgac ctattgcaag cctcattgct atgtatacag attctgcaat
     7021 ctgttcatca gtagcgccaa gttctttgga ttttattgtt ctttctcgta tgtcatgtgc
     7081 agattcggtt atgtaggcgc aggcaagagc aattagctgt ttgtcttttg cgcttataag
     7141 tccctctttg aaaacttcgt cacgaaatcc accccatgtt tcgtttaact ccggcaccag
     7201 attttcgaat ttgctgatgt acttatttac ctcgtcgtaa ttttcactca taatctttct
     7261 ccttcttgtt ttatttatat atatattaca taacgttaaa aaaattaaga aataactatc
     7321 tttccaaaat gcgagccgct ttttaaataa tcaaaagatt cagtggcatt ctcaaattca
     7381 aatgtctttc caattacagg tttaatatca tttgcttcta ttactttgtt catggattca
     7441 aacatatctc tgcttccaac atatattccc tgtagtgtga gtctctttag cattatcatt
     7501 acaggggaag gattttgttc aggttgtccg gacagcacac ctattaaact tatgcgccca
     7561 ctaacttttg cagaagttat tgatttctcg agggtgcctg ccccaccgac ttctataaca
     7621 ttatctacgc cttgtccatc tgtgattttt aaaacttctt tttcccaatc cggatgctct
     7681 ttgtaattta tagtatgatc ggcccctaat tttctggcca tttccagctt ttcatcactg
     7741 cttgatgtga ttatagcttt tgcaccttca gcttttacga attgtaatgc aaaaattgat
     7801 actccccctg ttccaagcat gagaacggta tcatctggtt ttaccgggtt gaaattcagt
     7861 gcctgccatg ctgtcacagc tgcacatgga agggtagcag cctcatggta gtttagatta
     7921 tccggtattt taacaacgcc attttcattt aatacgactt tttcagcaag cacaccatct
     7981 atagcacctc caagagcaga acctatctgt tcatcagtta aatctccgct tatccaggtc
     8041 tgaaaaaata ttccggcaac cctgtcacct tttttaaatc ttgtgaccga attgccaacg
     8101 gagacaactt cacccgcgcc atcggatagt ggaattagtg gtggattggt ttttacttta
     8161 tcatttctat aatatccacc tctaagcata ctaaggtctc tgtaattgag tgaccatgct
     8221 ttcatatcta taagtacatc gtttggtcct atctcaggtt caggtatttc attaagattc
     8281 atttcatata atttatcttg ttttgaaatt tgataagctt tcatttttaa ctccttttat
     8341 tttttaaaaa gttgattata ttcggatagg ttactataat caactagtat acttttagta
     8401 accactaatt gaggaggaaa tatgagaact gaagcagata ctactgaaga taaaaaacaa
     8461 gtagcggatt tttccgatcc atgcatttta ccttgtccta ttgaaagagg tatgaggata
     8521 attggtggaa agtggaaagg gtcgatactg tggtatctaa aagacggccc catacgattt
     8581 aatgaacttg ctaggaaatt aaaaggcgca agtaagaaga tggttaacca aaggttaaaa
     8641 gagatggaag atattggttt gctcaaacgc agggtaataa gtacaaaacc gatagctgtg
     8701 gaatacgaga taactgattt cggaagatca gcgctagtgt ttttaaatga attaaaagac
     8761 tggtctcttg agaataatat ttaaaaatct ggttaacttt aacatgtaag agtacatgct
     8821 aatttatcaa aacaatggaa ataacagttt gttaaagaat aaaagtaatt tctggggctc
     8881 ttatatactc ttcgaactcg ggagtttgta aaaatgattg aaaatagttc ggagattggt
     8941 agtttattat cataaaagta agattcaagc tgaattaagg ttactttaat catatggtat
     9001 actgcctatt ccattatgga aatagtaatt cccctcattg ccgacctaga ccaaaaccag
     9061 aaatatatac ttatgtgcca aggtgaagaa aaacaacaaa tcagacttca cgattataaa
     9121 accatatttt caattccggg tttatatgaa aaaatagttt atgaaattct ggaatgtaat
     9181 taaactaaag tagttgcgaa acttctacaa gaagaaatgt ccaaggaatc tgaaaacatt
     9241 tctgatataa cagtattgga tatgggtgct ggtaatggta tcgtagcaga ggagctggtt
     9301 aacttgggtt tagatgatgt gataggggtt gatattatag aagaagctaa gcaggctgca
     9361 tttagagaca gaccagaagt atatagcgac tacatatttg atgatttgac tttgcctgat
     9421 caaactacac aaagatttta gatgaaaaga gtattgattg tgtaacatgc gtggcagcca
     9481 taggctacgg agatatccca cccaaaacac ttgccaatgc aattaactat acagacgagc
     9541 aggggtgggt tgcgttatgt gttaaagaag attttttaaa agaggatgac agctatggtg
     9601 tctgtgaact atatgaagaa ttaattcaac aagacgtaat ggatataaaa aagaaaaaaa
     9661 attatatgca cagaaaatcg atatctggag aaaggttgat gtatgatgcc attattacca
     9721 agaaaaacac agatattcct cagtagatat taaacaatct ataaaaaagt aatttcttga
     9781 gttcttgtat acccttcgaa taaataggtt cggggttgtt atagtaaata gttcttatat
     9841 aagtaataca ttctaatgaa tttaactatc ttggcaatta cttgctatct ataataatcc
     9901 gttacaataa taagcattac ctaatatcac tatatgtgat ccaaatataa atctggttta
     9961 aacaatcaaa caattaagaa tgatattaaa tctaatttat ttgaattaca tttaacagta
    10021 attgtaaaaa tttataacag ggggggcaaa ttggttttta ctgaattgat aaaaaataca
    10081 tatctaggaa acagcctgca aaactggctg attgctatca ctgtggctat cgttttgagt
    10141 ctcatactta actcaataaa taaatatctt ttcctcaaac tcagtaagca cgctgaaaat
    10201 acatcaacca aacttgatga tatcctagtt ggctttttga gtaaaactaa ttttatgctt
    10261 gtgatagttt tttcaatctt tgtgggatct ctgtttcttg atttaaacaa gaccttactt
    10321 gtaataagaa aaagtgtaat aatcatagca tttttagtac agacggcact atggggtagt
    10381 ggtgtaatta cctttatagt aaatagaaag cttaataagt atagcttcga aacatcccgt
    10441 acagctatta ctcaaattaa atctatgggt cttgtgtcaa agattgtact atgggttgtt
    10501 atttttcttc taattctaga caatctggga ttcaatataa cgactcttat agcgggtctg
    10561 ggtataggag gtatagctgt agctcttgca ttacaaaatg ttctggggga tttaattgcc
    10621 tctttatcta ttatagttga caaacctttt gttgttggag acttcatcat tcttgatgac
    10681 tttttaggga atgttgaaca tattggtctt aaaactacta gagtaagaag cctttccggt
    10741 gagcagatta tattttccaa taatgattta ttgagcagca gactcagaaa ctacaagaga
    10801 atgtatgaaa ggaggattgt tttcactatt ggggtaatat atcaaacaac agctgaaatg
    10861 atagaggcaa tacctaaatg gataaaagaa attgttgatt ctgagccaaa tgcaagattt
    10921 gacagatcac acttttcatc ttatggcgat ttttccctaa atttcgagac tgtatatttt
    10981 gtagaatccc cggactataa tgtctacatg gatattcaac aaaatatcaa tatgggtata
    11041 ttcagaaaat ttgaacagga aagggttgag tttgcatatc ctacacaaac gttatttctt
    11101 gctagaaatg tctccaattc agaattaagc aataactcat aattcactac taaggagagg
    11161 acgttttctt atgggattta ttgtgcagca ttaatattga aatagctaaa cctgaaaagt
    11221 aatttctggg gctcttgtat actcttcgaa ctcgggggaa tttgcataac atcaaatttg
    11281 aatgatcaag tatcgattat ctatagaaat tgatcttgat ataaattaga gaatatatta
    11341 atgttagctt tagagatttt gtatttgtca gattatattt ttgagtatct ataataagtt
    11401 aatcgttttc ttcattactc atctaaatgg agttagagca aaacaccgca taggtgcaga
    11461 agatgagaac tatggacata ctacaaatac ttgctaacac cgacgatgga gttttcgccg
    11521 ttgggaccga ccataaaata gttttctgga ataagccggc tcaaaaaata ttgggttact
    11581 cggctaatga tgtgcttgga aaatattgta acgaaataat tgctggtaca gataacagtg
    11641 gaaatccggt atgttctaaa agctgtaatg taatgaaagc aataaaaaaa gaggaaaatc
    11701 ctcaaaatta tgatctgatt actcattcaa aaaaaggtga ccctatttgg cttaatgtaa
    11761 gcataatatg tattccggga agccagcctg cactcagcac agtagttcat atatttcatc
    11821 atattactaa gcagaagttg gaatttcatc gtattagaga ggcggtatcg gaaataaaca
    11881 atgaaagtaa actggagaat aaaaatttat caaagaatca atctaagaca caggggacac
    11941 taaccccccg tgaacgtaag gttcttaaat acctggaaga agggtgtaga cctgtagaca
    12001 taagtgaaaa actttatatt agtttatcta cactgcgaaa gcatattcaa aatatacata
    12061 ccaaactgga agtacattcc atgcttgagg cagttgctaa agcccggcag aacaatttga
    12121 tttaaatcac tcctctataa tatccaaata acctataaat caagcaaaaa tactcaattt
    12181 tgagtattta aaatccattt ttaaattggt agcattaaga aaagtacatt ttgaattggt
    12241 ataaagtatg ataacggggg tgattccgat gtgcattatg taatattaat cttaagaaat
    12301 tcaaaccgtt ttctatacct caattaaata attacaatct caatatacga tctttaaata
    12361 tatatcctat taaacggagg aaaaagtgag aaaaataata tatacattgt tgtttttaaa
    12421 catagcagta ttgatcgggt atcatgctct atatgcagct tctgcttcaa ttgggaatca
    12481 ttcctgtata ggacctgatg cctgtacttc caacaccggg gatataggta ataactcatg
    12541 caatggagaa gaagcatgtg atgagaatga aggggatgta ggaaataaat catgtcatgg
    12601 tttagaagcc tgtgcggata acaacgggga tgtaggaaac aactcatgta atagtcaaga
    12661 tgcgtgtcag gtaaatgacg ggagtgtagg acataattca tgtaatggta cagatgcatg
    12721 tggatctaac ggcggggata taggaaacag atcgtgcaat gatgattttg catgtacatc
    12781 caatgatgca gacgtaggag ataattcgtg ccgtagtatg gatgcctgcg atagagcggg
    12841 aagagacggc atgggagata caatcggaaa ttactcttgt attggttata aatcatgtga
    12901 agacaatgat gcatcatcca cagggatcaa ttcatgccat ggggagcatg catgtgatga
    12961 cgttactgat ggaataacaa ttggaaataa ctcatgtaat ggagatagag catgcgcttg
    13021 tcgaaccacc aatgtacccg ataatacatg taatggggaa gaggcttgtt ttgagtgctg
    13081 ataagtgata attatttcta accacaaagc ccacaatcag agctaacccc tgtggttgtg
    13141 ggtttttttg tgccgtttta agattgcaca aatattgaaa aataatttct ggggctcttg
    13201 tatacactcc gaactcaaaa gaatgcatat ttgttagaaa ataagactga tataatcgtt
    13261 tttatcaatc ttaatgcact tcattatgat tagtaggcgt cacataatat gctaaactcg
    13321 tgtatataaa aataaatgcg ttttagatat tacaacatac ataagctata ttccactgat
    13381 tcagaatttt cagatatcat tataaccaca accttgtttc agtaaactcc ttttcctttg
    13441 tatactgaac attgcaaata ccaaaatatt aagtagacat atttctgtag ttaatgtctg
    13501 aaaaatgatt tcaaggagta atattatggt caaggttgct atagtacagg aatctcctgc
    13561 atttctaaat aaagtaaaaa caattaaaaa ggcagttgct ttaatcaaac aagctgcaaa
    13621 ctctggtgct cagttaatag tttttccaga agcttttatt ccagcttatc ctgcttggat
    13681 atggcgactg agaccgggcg gtgactggaa cattagcgag aagttatatg cgagtttatt
    13741 agataattct gttaatctag actcagacga tatctcccca atatgcaagg tagcaaaaaa
    13801 acataaaata acggtagtat gcggtttaaa tgaaaaagac agcaaattca gtcaagcaac
    13861 tctttacaac acagtaataa taattgggtc tgatggtgca atccttaacc gacatcgtaa
    13921 attaatgcca actaacccgg aaagaatgat atggggtttt ggagatgggt cgggcttaaa
    13981 ggttgttgat acaactatag gaaagattag tacattgtta tgctgggaaa actatatgcc
    14041 tcttgcaaga tatgctctgt actcacaagg tgtggaaatc tatatagcac caacatatga
    14101 cagcggggat ggctggatag gaacactgca gcacattgcc cgcgagggga ggtgctgggt
    14161 agtttgcagc ggagtagctt ttgaaaattc ggatataccg ccagattttc cagagaaaaa
    14221 agctttatat ccagattcag aagattggat taatcccgga gattctgtag tcataaaacc
    14281 gggaggagaa attgtggctg gaccgatgca taaagaaaaa ggaatacttt atgctgatct
    14341 ggatataaaa ctcgtcggga ctgccaaaag aacacttgac gttgcgggtc attattctcg
    14401 ccctgacata tttaaactta gcgtaaatac aaacccgcaa tctccggtta aattcgaata
    14461 gtaatgacat taaaacaata aggaggtgca taatgagtaa agagaaaact tacacaggta
    14521 actgtttctg tggagaagtt gagttcacgg taacaggtga gccagcagtc atgggctatt
    14581 gccattgcga ctcgtgccgg cattggtcag caggtcctgt taatgccttc acattatgga
    14641 taccagaagc tgtggaggtt ataaaaggaa aagataacat agacacttac aacaagacac
    14701 cccagagcta tcgaaagtgg tgtaagacct gtggaggcca tatcttcact gagcatccgg
    14761 gcatggggtt catagatgta tatgcagtct tgattcagga ttttcccttc aagcctggtt
    14821 ttcacgtaaa ctatcaagag acagtgctgc atatcaagga tggtctgccg aagcttaggg
    14881 atttacccaa agaagccggg ggctcgggag aggtattgcc tgaatagagt accgagtgaa
    14941 acttttaact gcactttaag atcaagtgat tatgagtagt atcctaatta gttctaacct
    15001 cataaactat aggattaatt gactaacagt aaaccaaacc cacaatcaga gttcgttgct
    15061 ttggttgtgg gttttttatt ttgaagttaa gtagctaaaa ctgaaaaata atttctgggc
    15121 tcttgtatac acttcgaact agtaagtact accagagcat ttaatctata gatactatag
    15181 tattgaaatt taattaatga tattaaaact cataaaaagc ctgttgtaga atatgtaaat
    15241 tccacaacag gctatgattt attttatcat ttttaagaat gagtcttaaa actcataaaa
    15301 ctaaactatc aatctgacct tgccccatat aatagtacca gattataagt tagaatttta
    15361 ggtaatgacc ctcacccaaa agctgtaccg ggataattag agatttccgg cttttttatg
    15421 tgttgtttaa catactcctc cggcgtcata ttatcaagtg ccgaatgagg tctgtactcg
    15481 ttgtaatctc
//
LOCUS       DBNGSV010000084        14886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_292842, whole genome shotgun sequence.
ACCESSION   DBNGSV010000084 DBNGSV010000000
VERSION     DBNGSV010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_292842"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="AC159U_03010"
     CDS             complement(<1..92)
                     /locus_tag="AC159U_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3336059.1"
                     /translation="MSKQKFERGKPHLNIGTVGHVDHGKTTLTAA"
     gene            complement(140..212)
                     /locus_tag="AC159U_03015"
     tRNA            complement(140..212)
                     /locus_tag="AC159U_03015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(177..179),aa:Thr,seq:ggt)
     gene            complement(223..296)
                     /locus_tag="AC159U_03020"
     tRNA            complement(223..296)
                     /locus_tag="AC159U_03020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Gly,seq:tcc)
     gene            complement(351..432)
                     /locus_tag="AC159U_03025"
     tRNA            complement(351..432)
                     /locus_tag="AC159U_03025"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Tyr,seq:gta)
     gene            complement(517..588)
                     /locus_tag="AC159U_03030"
     tRNA            complement(517..588)
                     /locus_tag="AC159U_03030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(554..556),aa:Thr,seq:tgt)
     gene            complement(778..1212)
                     /gene="fabZ"
                     /locus_tag="AC159U_03035"
     CDS             complement(778..1212)
                     /gene="fabZ"
                     /locus_tag="AC159U_03035"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3336060.1"
                     /translation="MDIDYIKSILPHRAPFLFIDRIISIDKGEKIEAIRKFSRDEYFF
                     EGHFPGSPVVPGVIIVEALAQVGGILVYESFKEDIKDMLPALAGLESVRFKKPVVPDD
                     EVILSVNLLKARSRLWKLSGEAKVGDSVVAQAEILASVFAKQ"
     gene            complement(1258..1773)
                     /locus_tag="AC159U_03040"
     CDS             complement(1258..1773)
                     /locus_tag="AC159U_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3336061.1"
                     /translation="MRKVITVLAFMVFSISGAMFADAATKIGYIDLQSVIQNSKAGKA
                     AKSSFEVEFNRKRQIIENKAKVLAQEEQEFINQSPLMDSEARKRRAEELNKKKKELAR
                     LRDDFRDELQKKDFELTQKILKELESVIKNIGTSEGYSLIVEKTESGVIYGNDTVDIT
                     QKVIKAYDQKN"
     gene            complement(1795..4047)
                     /gene="bamA"
                     /locus_tag="AC159U_03045"
     CDS             complement(1795..4047)
                     /gene="bamA"
                     /locus_tag="AC159U_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR3336062.1"
                     /translation="MLIGLVLSPTPLMSSDGSQIIEVIVVGNRRIETQLIESKVSVSA
                     GDKLSSSAVSDDIKSIFKLGYFDDVVAEIDQTDDGVILIYKLTEKPVVVDIRINGNDE
                     LSTETITDVLSLNEGQIVDITSLNNTLDSIRKLYSERGIIPTEVDYSIEPRGEGTIEL
                     NIDIKEGKKSYIKKVQFEGNDSIKSDEILEKIYSKPKSIISFITSRGLYRPEEIERDS
                     DRIRAVYLDNGYLDVNVSKPEKVYSEEKEGHIITFRVEEGPQYTVSSVSVNGDLIVTK
                     EELVGLLKLKQGEIFKSSYVGDDINILTTYYGDKGYAFANVDPRIIPNKETLDVDATF
                     TIEKGKEVYVRRIDIAGNERTRDKVIRREIPIQEQSLYNATQIRSIKPRIFRRGYFED
                     DVQVDTERVPGADDQVDLNVNVKEKPTGFFSVAGGFSSVETVLFSGQIQESNLFGYGK
                     QISLSAQIGGVTQLFSLNYSDPVFLDSDWNMNARIFRTDREFRDFDRKSFGAALTFGR
                     RLWRFLRGEVGYRFENQDIGDVFGDARLIITESSRTLSSLSAGFVWDSRNNFLDPTGG
                     LVARTSIEYGGPFGGDTDFVKYRASAKYFYPAWKSTVLAFTGRYGYLNLMDVGNDLVV
                     GERFFLGGPNSLRGFKFRRVGARVPTDDGDFVIIGGTQELLISAEYIVPLLKQVNVKG
                     VLFVDVGNAYNDGEELSLDPGDLRRNVGFGFRWLSPLGPLKLDIGFPIGDRLEDEDSF
                     EIQFTVGNLF"
     gene            complement(4082..4762)
                     /locus_tag="AC159U_03050"
     CDS             complement(4082..4762)
                     /locus_tag="AC159U_03050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3336063.1"
                     /translation="MIYLIEVSDLWKVYETNGSKVQALRGIDLKIDQGQSLAIEGVSG
                     SGKSTLLHLMGALDHPSKGDIKYDGEDLLDKSDSEIAKFRNNHIGFVFQFHYLLAEFN
                     ALENVMMPALIKGMNKTEAEQLALKGLEDVGLGDRVTHRPGELSGGEQQRVAIARSII
                     LKPNVIFADEPTGNLDQKTGSSIIDLFVKLNKEYNVSIIVVTHNTYVSSYFDRKINLL
                     DGKIVNEN"
     gene            complement(4769..6067)
                     /locus_tag="AC159U_03055"
     CDS             complement(4769..6067)
                     /locus_tag="AC159U_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3336064.1"
                     /translation="MRYEFFIGLRYLKSRNKQSFVSIIGLISIVGVIIGVMSLNVVLG
                     VMKGFADELREKILGVNAHVVVLNYNGAFEGYDELTKKISEYKGVTGASPFVYGQGML
                     VAGSNVTGAVVRGIKPESAGKVINIYEAIGKGSDFDRNKKTVEQHREYGKELLGKLDH
                     KTLSGYPPIIIGKELAASLGVIIGDEINLVSPFGKIGPLGHIPKVRKFEVLAIFDYGM
                     LEYDSSISYVAIKDAMKFFDMKDKVSGIELKSENAFEAKSLSAELKDHLGYPFYTRNW
                     EDVNQSLFKALKLERIAIGIFLTFIILVAALDIISTLTMVVMEKNRDIAILKAMGASR
                     NSILNIFLTEGMIIGFVGTTIGTILGYLICYLLKTSEFVKGLIPFDPHVYYVSGFPVK
                     IEPIYFIGVAVTSLLICLLATLYPSFQASRKDPVEALRYE"
     gene            complement(6064..7527)
                     /gene="lysS"
                     /locus_tag="AC159U_03060"
     CDS             complement(6064..7527)
                     /gene="lysS"
                     /locus_tag="AC159U_03060"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3336065.1"
                     /translation="MENDLTLQRINKVNELIEQGVNPYPNDFKPANMTSELIDKYSDK
                     DGEQLKAMEAEFSIAGRVISIRDFGKSAFFHVKDSSGKMQCFIQKNQIGEEKIKFFKK
                     FIDIGDFVGVTGKLFKTKTEELTLNTTEIVIITKSLKTLPEKWHGLKDVELRYRQRYL
                     DLISNEGIKDIFLNRSKIIKLIRKYMDDRGYVEVETPILHPIAGGATAKPFKTHHNAL
                     DMELYLRIAPELYLKRLVVGGIEKVYEIGRTFRNEGISTQHNPEFTMIEFYQSYSTYT
                     DLMDLIEDLIIHVTRELLGTLQIDYVDTKIDLSAPWPRINIMESLKETLSDKEFSDEQ
                     ALFKKADSMGLEHNNIRGKALMEIFETVTGEKLNNPTFVYGFPLDISPLARKNDDDPT
                     ITDRFELYISGKEIANAFSELNDPQDQKQRFENQIEQKSKGEEEYHEMDSDYVHCLEY
                     GMPPTAGAGIGIDRLVMLLTNSPSIREVLLFPHLRPE"
     gene            complement(7654..8847)
                     /locus_tag="AC159U_03065"
     CDS             complement(7654..8847)
                     /locus_tag="AC159U_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3336066.1"
                     /translation="MPNILKFNSRLYNKNFILLTASSFLFFFNMHAFVLLPVWIKELG
                     GSESDIGYIMGATSFSTIFSTPFVGYLVDKHDKKKLLFMGTTLLALTSLPLVLISNLV
                     SLIYALRIMQGFAFSLFFVSAGTLVTEVSPKENRTEALGIFGVFTIINYAFAPFIGRK
                     IVEYFDFNYFFILISIVGLLALPFILNIKIARTSDNEKLSEEDVSLTKILKRPAILLS
                     AFTLLIAGSGFIPTLSFLPVFSQKLKIMEFEIFFISYTFAVLFIRLVVGWIPDRIGKI
                     KTVIPSLVIFTASIIYLGFSEQKVDLILCGLLFGFGHGFLYPTLYSIVIDNSRLSERG
                     KVFSICSVAFTLGGMLGAFSYGIVAENYGYAYMYKLIGSICLLGFLVFILYYIKELFK
                     FSHVK"
     gene            9008..10363
                     /locus_tag="AC159U_03070"
     CDS             9008..10363
                     /locus_tag="AC159U_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3336067.1"
                     /translation="MCLNLPFRVFSSLKSISIYSVAVLVMLLLPETGLGQDTAVLDTG
                     DTAWILISTGLVLFMMIPGLAMFYAGLVGRKNVLSLFMQCFAITGVITVLWVLFGYSM
                     AFDTTGMQEGVRNLNSFVGGFSKAMLAGIGIDTLSGTIPETVFVTFQMTFAIITPGLF
                     IGAFAERMKFTAIMAFVIFWAILVYFPITHMVWSGSGSFLGDMGIIDFAGGIVVHITA
                     GVAALFAAIMVGKRRGYPTTIRPPHNMTLTLTGTAMLWVGWFGFNGGSALAANGQAGM
                     AVLVTQISPCLAALTWITIEWIKAGKPSALGFATGAIAGLAAITPASGSVGPLGAIVI
                     GISSGFLANIASTSIKQKLGYDDALDVVGVHGVGGLIGILLVAFFGSPLLGGTVEGLV
                     MSKQFGIQAFGAVFTIIYTAIFSYIILKVIDMVIGLRVDDESELQGLDLAEHGESGYD
                     L"
     gene            complement(10439..11320)
                     /locus_tag="AC159U_03075"
     CDS             complement(10439..11320)
                     /locus_tag="AC159U_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIR3336068.1"
                     /translation="MSSDLKDIKIPKFNFIIPVIIAIIVMVVNSYVTVQSGHVGVVRT
                     FGAVQPKALPEGFHFKKPFIDKVEVMDVRLTKAHAQAASASKDLQTVKTQVTVQYSLV
                     GAVAPKTYQKIGVRQVVAVTLIEPAIQESVKAITAQYTAEELVTKREEVKIRIQEAIN
                     NFIRITLKDKQAENALTIANVAITDFDFSNEFNKAIELKVRAEQEALQAKNEKIRRIT
                     QAEAAAEEKKLAAEAEAFQIEIASKARADAIAREAKALRGNPALIQLRIAEKWNGVLP
                     KFTGGDAIPLLNIDPDK"
     gene            complement(11452..12735)
                     /locus_tag="AC159U_03080"
     CDS             complement(11452..12735)
                     /locus_tag="AC159U_03080"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3336069.1"
                     /translation="MNLILENGTIIDPSQNINKKGNVLIEEGRIKGFHNKITESKYKG
                     FKKIDCKGKIISPGLIDLHVHLREPGFEHKETIKTGCEAAAAGGFTTIVCMPNTNPVN
                     DNASVTEFILYKARTEGIVNVLPIGAITIGEQGESLAHIGEMYEAGCVGISDDGMPVM
                     HSGIMRNAMEYVKAFDIPVLIHAEDKGLSCDGVMNEGVTSTTLGLKGIPNASEEVMIA
                     RDINLSELTGTRLHVCHVSTEGSVRIIREAKSRGVKVTAEATPHHFHLTDEIVKDYNT
                     FAKVNPPIRTAKDVDAIREGLSDGTIDAIATDHAPHSEDEKKVEFDKAPFGITGLETA
                     LQLSLQLVEQKVISIDDMVSKLTHIPAAIIGSDRGTLKEGSVADVVIFNTIDESVIDR
                     HLFRSKGKNTPFHDRVVKGKVNYTIVGGNIVYENL"
     gene            complement(12732..13661)
                     /locus_tag="AC159U_03085"
     CDS             complement(12732..13661)
                     /locus_tag="AC159U_03085"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3336070.1"
                     /translation="MGFSKGHILGISEMSAKDINEILKTAESMKVVSSRNIKKVPTLK
                     GITVVNLFFEPSTRTRSSFEIAEKRLSADVLNLSLDQSSVQKGETLLDTIKNLKAMGA
                     SIIVIRHPMSGAPWLLAKELDCSIINAGDGSHEHPSQSLIDLFTVKERKKRIKGLNVV
                     IVGDILHSRVARSNIWGFNKLGANVSVVGPPTVIPKYISELGVKHYYSLEQSLKGADV
                     IIMLRIQKERQNTNLFPSLREYSKFYGLTRQRLNLANDDVILMHPGPINRGIELSSEL
                     ADNSKSVILEQVANGIAVRMSMLYHVVQKRGEN"
     gene            complement(13664..14197)
                     /gene="pyrR"
                     /locus_tag="AC159U_03090"
     CDS             complement(13664..14197)
                     /gene="pyrR"
                     /locus_tag="AC159U_03090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3336071.1"
                     /translation="MNKDLLMDVEDIRTAITKISELLQESINDFQNVCIVGIRTRGIV
                     VAQRILKQLNTNNINNIPLGILDITLYRDDLRTKDTWPTIEKTDIDFDINNKDVVIVD
                     DVIYTGRTARAAIEAVMDYGRPSSIKLITLVDRGNRELPIQPDFCGAVFDIAGGKRVN
                     VFLNDTDGREGVEVTTT"
     gene            complement(14212..14562)
                     /locus_tag="AC159U_03095"
     CDS             complement(14212..14562)
                     /locus_tag="AC159U_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336072.1"
                     /translation="MGAKNVFTVIIVCVLLFVAFKVGPILYKGVFGIRGICAEQVDRY
                     KKYGSQFVFMRVDEDLRRIGIPKQNSKYQIKIEGEKVYLDISYWDTAHFYKKYKKDFE
                     FHHQCFSDTDTLFK"
     gene            complement(14582..>14886)
                     /gene="lepB"
                     /locus_tag="AC159U_03100"
     CDS             complement(14582..>14886)
                     /gene="lepB"
                     /locus_tag="AC159U_03100"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3336073.1"
                     /translation="DTTYVDEYEEDLFNHKHKVIYRNGKESTDKGIFIPFDKVPEGHY
                     FVMGDNRDNSKDSRYWGLVPFENISGKALLTHWSWDDRNSKLISDVRWERIFKLIK"
BASE COUNT         4351 a         3113 c         2385 g         5037 t
ORIGIN      
        1 gctgctgtaa gagtagtctt cccgtggtct acgtgtccca ctgtgcctat gtttaagtgt
       61 ggtttgcctc gctcaaattt ttgctttgac atcttactcc ttacctcctg cttttgttta
      121 aaatttatct aatcaaaata gcccacgatc ggacttgaac cgacgacctc atccttacca
      181 aggatgtgct ctaccgactg agctacgtgg gcagagtttt cgagcgggag acgggattcg
      241 aacccgcgac cctcagcttg gaaggctgat gctctagcca actgagctac tcccgcggta
      301 aaatattttt tatgaatata tttcatttca agttttatat tgattattta tggggagggc
      361 aggattcgaa cctacgtagg cattgccgac agatttacag tctgctgctt ttgaccactc
      421 agccacctcc cctatatccc aatttttttt atttttattt atttattaat atgtttttct
      481 aatttggttt taatcttaat gagctattaa tttaagagct ggcgaaggga tttgaacccc
      541 cgacctgctg attacaaatc agctgctctt ccactgagct acgccagctg attaatttaa
      601 aaaatataaa ccactcctgt tttgaattaa gcggaatatc ataacagtag gtattgcttt
      661 gtcaaataga aatttaagaa aaaaacagtt gctgtaaaga gaattattac gcaacaaaac
      721 aaatcagtta attatgtaat ttatttatca aatcctaaaa agacattcat attcactcta
      781 ttgcttggca aatacagaag ctaagatttc agcctgtgct acaaccgaat ctccaacctt
      841 agcctctcca ctaagtttcc atagtctgga tctggccttt agaaggttca cacttaatat
      901 gacttcatca tctggaacaa ccggtttttt aaacctcaca ctttccagcc ctgcaagtgc
      961 aggaagcata tcttttatat cttccttaaa gctttcatag accaatatgc ccccaacctg
     1021 tgctaaggct tctacaatta taacccccgg cactacgggg cttccgggaa aatggccttc
     1081 gaagaaatat tcatcacgtg aaaacttcct tatagcctct attttttcac ctttatctat
     1141 acttattatt ctatctataa ataaaaaagg ggccctgtga ggcagaatgc tttttatata
     1201 atctatatcc attatagtat ttttaacaat tattaaagat aagtattata aagatattta
     1261 gtttttctgg tcatatgctt ttatgacttt ttgtgtgata tccacagtat cattgccata
     1321 aataacaccg ctttcggttt tttctactat taaggaataa ccttcacttg taccaatatt
     1381 ctttataacg ctttcaagtt cttttagtat tttctgtgta agttcaaaat cttttttctg
     1441 aagttcatct ctaaaatcat cccttagcct tgcaagctct tttttctttt tgttaagttc
     1501 ctcagccctt ctctttcttg cttcagaatc cataagtggt gattggttaa taaactcctg
     1561 ttcttcctga gcgagcactt tggctttatt ttcaataatc tgtctttttc tattaaactc
     1621 tacttcaaaa gaagattttg ccgcttttcc tgctttagag ttttgaataa cactttgtag
     1681 gtctatataa cctatctttg tagcagcatc tgcaaacatt gctccagata tgctaaatac
     1741 cataaatgcc aaaactgtaa taacttttct cattcttgcc tcctgatttt attcttagaa
     1801 taaatttcct acagtaaatt gtatttcaaa gctgtcctca tcttcgagtc tgtctccaat
     1861 gggaaagcca atatcaagtt tcaaagggcc tagtggtgat aaccacctga acccaaagcc
     1921 tacatttctt cttaaatcgc ccggatcaag actaagttct tctccgtcat tataagcatt
     1981 accaacatct acgaatagta ctcccttaac attaacctgt tttagtaaag gaactatata
     2041 ctctgcagat attagaagtt cctgagtacc accgataatt acaaaatcac catcatctgt
     2101 tggtactctg gcaccaaccc ttctaaactt aaatcctcta agtgagtttg ggccgcccag
     2161 gaaaaatctt tcaccaacca caaggtcatt accaacatcc ataagattca aatatccata
     2221 tctacctgta aatgccagca ctgtactctt ccatgcagga taaaaatact ttgcacttgc
     2281 cctgtactta acaaaatcag tatcacctcc aaacgggcct ccatattcga tggatgtcct
     2341 tgcaacaaga cctccggtag gatctagaaa attgtttcta gaatcccata caaaaccagc
     2401 tgaaagactg cttagggttc tggatgattc tgtaataata agtctagcat caccaaaaac
     2461 atcaccaatg tcctggtttt caaacctgta gcctacctct cctcttagaa atctccatag
     2521 tcttcttcca aaagtaagcg ctgcgccaaa ggattttctg tcaaaatccc taaactctct
     2581 gtcagttcta aaaattctgg cgttcatatt ccagtcagaa tcaaggaata ccggatcaga
     2641 gtagtttaat gaaaacagct gtgttactcc tcctatctgg gctgataatg aaatctgttt
     2701 accatagcca aataggtttg actcttgaat ctgtccggag aaaagcacag tttctacaga
     2761 gctaaaccct ccagcaacgc taaaaaatcc agttggtttt tcttttacat taacattgag
     2821 gtcaacctga tcatctgcac cgggcactct ttcagtatcc acctggacat catcttcaaa
     2881 ataacctctt ctaaaaatac gtggtttaat tgagcgtatt tgagttgcat tatacaaact
     2941 ttgttcctga ataggaatct ctcttcttat tactttatct ctagttcttt catttccagc
     3001 aatatcaatc cttctcacat agacttcttt acctttttct atagtaaaag tagcatcaac
     3061 atctaatgtc tctttatttg gtattatcct gggatcaaca ttagcaaatg cataaccctt
     3121 atcaccgtag tatgttgtta gtatattaat atcgtctcca acataactgc ttttaaatat
     3181 ttcaccctgt ttcaatttta ataaaccaac cagctcttct tttgttacaa tcaaatctcc
     3241 attcacacta acactgctca ctgtatattg aggaccctct tctaccctaa atgtaattat
     3301 atggccctct ttctcctcac tgtacacttt ttcaggtttg cttacattta catccagata
     3361 tccgttatca aggtaaacag cacgaatcct gtccgagtcc ctttcgattt cttctggtct
     3421 gtaaagcccc cttgaagtaa tgaatgagat aatgcttttt ggcttggagt aaatcttttc
     3481 cagtatttca tcagatttaa ttgaatcatt tccctcaaac tgcacttttt tgatataaga
     3541 tttctttcct tccttaatat ctatattgag ctcaattgtt ccttctcctc tgggctcaat
     3601 ggaataatca acctctgtag gtattatgcc tctttcagag taaagttttc taattgaatc
     3661 gagagtatta ttaagactgg taatatcaac gatctgccct tcattcaaac taagaacatc
     3721 tgttattgtt tcagtactaa gttcgtcatt tccattaatt ctaatatcta caaccactgg
     3781 tttttctgta agtttataga tgaggataac accatcatct gtttgatcta tttcagcaac
     3841 aacatcatcg aaatatccca gtttaaagat actctttata tcatcgctca cagcactgct
     3901 tgatagctta tcgcctgcag ataccgatac cttagattca ataagctgtg tctctatcct
     3961 tctattacca accacaatta cttctattat ctgactgccg tcagaggaca tgaggggagt
     4021 gggactaaga actagtccaa ttagcatggt atgcaataac actaaatata tttttgcaga
     4081 attaattctc atttacgatc ttgccatcaa gcaaattaat ttttctatca aaatatgagg
     4141 acacatatgt attatgagtt actacaatta tggatacatt atactctttg tttaatttga
     4201 caaataaatc tataatagac gatccagttt tttgatcaag gttacctgtt ggttcatctg
     4261 caaaaatcac atttggtttt aatataatcg atcttgctat ggcaaccctt tgctgctctc
     4321 cacctgaaag ttcacctggt ctgtgagtaa ccctatcacc aagcccaaca tcctccagcc
     4381 cttttaatgc aagctgttca gcttctgttt tattcatccc tttaattagt gcgggcatca
     4441 taacattttc caaggcatta aattcagcta aaagataatg aaactggaaa acaaatccaa
     4501 tatgattatt tctaaattta gcgatctcac tgtctgactt atccagcaaa tcttcgccat
     4561 catactttat atcacctttt gaaggatgat caagtgcgcc cattaaatgc agtagagtgc
     4621 ttttccctga gcctgatacc ccctcaatag caagtgactg gccctgatca atctttaggt
     4681 caatccccct tagagcctgc acctttgagc cattggtttc atataccttc cataaatcag
     4741 atacttctat caaataaatt attctccctt attcatatct aagtgcttca acaggatctt
     4801 tcctcgaagc ctgaaacgat ggatatagag ttgccaataa acatattaat aaacttgtta
     4861 cagctacacc tataaaataa atcggttcaa ttttaactgg aaatccagaa acataataga
     4921 catggggatc aaatgggatc aagcccttaa caaattccga agtctttaat aaataacata
     4981 taagatatcc caaaatcgta ccaattgttg tacctacaaa tcctataatc atcccttctg
     5041 ttagaaatat attaagaata ctgtttcttg atgcgcccat tgcttttagg attgctatat
     5101 ccctgttttt ttccataaca accatggtta gcgtactaat aatatccagt gcggctacaa
     5161 ggataataaa agtaaggaat attccaatgg caattctctc aagcttaaga gccttaaaaa
     5221 gactctgatt tacatcctcc cagtttctgg tgtaaaaagg atatcctaaa tgatctttta
     5281 gctcggcact gagtgatttg gcttcaaaag cattttctga tttaagctca attcccgata
     5341 ccttatcttt catatcaaaa aacttcatag catctttgat tgcaacatat gatatagatg
     5401 aatcatactc cagcattcca taatcgaata ttgcaagaac ctcaaatttt cttacttttg
     5461 gaatatgtcc caatgggcca atcttgccaa aaggggacac caggtttatt tcatctccta
     5521 ttataactcc aagcgatgcc gcgagttcct ttcctattat tataggcgga tatccggata
     5581 gagttttgtg atccagcttg ccgaggagtt ccttgccgta ttctctatgt tgctctactg
     5641 tttttttgtt gcggtcgaag tcagaaccct tgccaatggc ttcataaata ttaataactt
     5701 tacctgcaga ctccggcttt atgcccctta cgactgcgcc cgtaacatta gaacctgcta
     5761 ctagcatccc ctgaccataa acaaacgggc ttgctccggt aacaccctta tattctgata
     5821 tttttttcgt aagctcatca tagccttcaa aagcgccatt atagtttaac actactacat
     5881 gagcattaac tcctagaatt ttctccctaa gctcatctgc aaagcctttc attacaccaa
     5941 gaactacatt tagagacata actccaatta ttacacccac gattgaaata agccctatga
     6001 tcgagacaaa gctctgctta tttctggatt ttagatatcg gagtccaatg aaaaattcgt
     6061 atctcattca ggtcttaggt gtggaaaaag tagtacttct cttattgagg gggagtttgt
     6121 aagaagcatg acaagcctgt ctattcctat acctgcacct gcagtgggag gcataccata
     6181 ctcaaggcag tgcacataat cagagtccat ttcatgatat tcttcttcac cctttgattt
     6241 ttgttcaatc tgattttcga atctttgttt ttgatcctga ggatcattta attctgaaaa
     6301 ggcattggct atctcttttc ctgatatata tagctcaaat ctgtcagtaa tagttggatc
     6361 atcatcattc tttcttgcaa gcggagaaat atccagagga aatccatata caaaagttgg
     6421 attgtttaac ttctcgccag taacagtttc aaatatctcc ataagagcct tacctctaat
     6481 attgttatgc tcaagaccca tcgaatctgc ttttttaaat agagcttgct catcactaaa
     6541 ctctttatcg cttagggttt cttttaaaga ctccataata tttattctcg gccacggagc
     6601 agacaaatct atcttggtgt caacataatc tatttgaaga gtgcctagta attcccttgt
     6661 aacgtggata ataagatctt ctattaagtc cataagatct gtataggttg aatatgactg
     6721 atagaattct atcattgtaa attccggatt atgctgtgta gagatgcctt catttctaaa
     6781 agtccttcca atttcgtaaa ctttttcaat accgccaact acaagccttt tgagatatag
     6841 ttcaggagct attcttagat aaagctccat atcaagggca ttgtggtggg ttttaaacgg
     6901 ttttgcggta gcccccccag ctataggatg caggatcggg gtttccacct ctacataacc
     6961 cctgtcatcc atatactttc ttattagttt aattattttt gatctattga gaaatatatc
     7021 ttttattccc tcattagata ttaagtctag gtaacgctgt ctgtaacgaa gttcaacatc
     7081 ttttagcccg tgccattttt caggaagtgt cttaagggac ttagtaataa ttactatttc
     7141 agttgtattt aatgtgagtt cttcagtttt tgttttaaac agttttccag ttacaccaac
     7201 aaaatcgcct atatctataa attttttaaa aaattttatt ttctcttcgc caatctgatt
     7261 tttttgtata aagcactgca tctttccact tgaatcttta acatgaaaaa aagcactctt
     7321 gccaaaatct ctaattgaaa taacccttcc cgcaatagaa aactcagcct ccatggcttt
     7381 tagttgttct ccatccttat cagagtattt atcgatcaac tccgaggtca tattggccgg
     7441 cttgaagtcg tttgggtaag gattaacccc ctgctcgata agctcattta ctttgtttat
     7501 tctttgtaga gttagatcgt tttccatatt tttaaaaata atattgagga ataaattaga
     7561 taattaaact ggctttgttt gaaaaagttc ctgtggatta ttaactattt tgtataacta
     7621 gtaaagataa tcagattact catgttttgc aaattatttg acatggctaa atttaaataa
     7681 ttctttaatg taatatagaa taaaaacaag aaatcctaaa aggcaaattg agcctataag
     7741 tttatacata tatgcgtagc cataattttc agcgactata ccataactaa aagctcctaa
     7801 catcccacca agagtaaaag caacactgca aattgaaaac actttacccc tttcacttaa
     7861 tctagaatta tcaataacaa ttgagtaaag cgtagggtat aaaaatccat gcccaaaccc
     7921 aaacaatagt ccgcaaagaa tcaaatcaac cttctgctca gaaaaaccaa gataaataat
     7981 actagctgta aaaataacaa gggatggtat aactgttttt atctttccaa ttctatcggg
     8041 aatccatcca accaccagtc ttatgaagag aactgcaaag gtatagctta taaaaaatat
     8101 ctcaaattcc atgattttta gtttttgaga gaaaaccgga agaaagctca aagtaggtat
     8161 aaatcccgac cctgctatta acaaagtaaa agctgaaagc aagatagcag gtcttttaag
     8221 aatttttgtt aaggatacgt cttcttcaga tagcttttca ttatcgcttg ttctggctat
     8281 tttaatattt aatataaatg gaagagccaa taatccgaca atagatatca gtataaaaaa
     8341 ataattgaaa tcaaaatact ctactatttt cctgccaatg aagggagcga atgcatagtt
     8401 tataattgta aatactccaa atatacccaa cgcctcggtc ctgttttctt taggagagac
     8461 ttctgtgaca agtgtgcctg ctgaaacaaa aaatagagaa aaagcaaaac cttgcataat
     8521 cctaagcgca tagattagcg atactagatt gcttattagg accaagggca agctagttaa
     8581 ggcaaggagc gtagtgccca taaatagaag cttcttctta tcatgtttat ctaccaaata
     8641 accaacaaat ggagtactga atattgtgct aaaactggta gcacccatta tatatcctat
     8701 atcactctca ctgcctccaa gttccttaat ccacacaggt aggagcacaa atgcgtgcat
     8761 attgaaaaaa aacaggaaag aacttgctgt tagcaatata aagttcttat tataaagcct
     8821 ggaattaaac tttaggatat taggcattga gaattcataa tcaaatggtt gtgttgatta
     8881 aatataaaat aaatattata atcagcaaag gatcaaggta cccaatctat atccgcagta
     8941 cagattttgc aataacttag taatagccag aatgaatcag ggtataaaag gatgtaagga
     9001 ggcaggtatg tgtttaaatt taccctttag agttttttca tctctaaaat caatttcaat
     9061 atattcggtg gcagtactcg taatgctatt attacccgaa acggggcttg gccaggatac
     9121 cgcagttctt gatacgggcg ataccgcttg gatcctgatt tctacagggc ttgttttatt
     9181 tatgatgata cctggtcttg cgatgtttta tgcaggtttg gttggcagaa aaaacgtgct
     9241 ttcactgttc atgcagtgtt tcgctattac aggtgtaata accgtattgt gggttttatt
     9301 tggttacagc atggcatttg atactacggg catgcaggaa ggagtaagaa atctaaattc
     9361 ttttgtaggt ggtttttcaa aggcaatgct tgcaggaatt ggaatagata ctctttcggg
     9421 aactattcct gagactgtgt ttgtcacatt ccagatgact tttgcaatca tcaccccagg
     9481 actttttatt ggagcatttg ctgaacgaat gaaattcaca gccataatgg catttgtaat
     9541 attctgggca atacttgttt atttccctat cactcatatg gtctggagcg gtagtggttc
     9601 atttctgggg gatatgggaa taatagactt tgccggtgga attgtggttc atattactgc
     9661 aggtgtagca gctttatttg cagctataat ggtaggtaaa agaagaggat atcctactac
     9721 tataagacct ccacataata tgacgcttac tctaacagga actgcaatgc tgtgggtagg
     9781 atggtttggt tttaatggag gaagtgccct tgctgcaaac ggccaggctg gtatggcggt
     9841 gcttgttact cagatttccc cttgtcttgc tgctcttaca tggataacta tagaatggat
     9901 aaaagcggga aagcctagcg ctcttggttt tgctacaggt gctattgcag ggcttgccgc
     9961 aatcaccccg gcctcaggat ctgttggccc gcttggcgct atagtaattg gtattagttc
    10021 gggatttctt gccaatattg cttcaacatc aataaaacaa aaactgggtt atgatgatgc
    10081 gcttgatgtt gtaggtgtac acggagtcgg cggccttatt ggtattttac ttgtagcatt
    10141 ttttggctct ccgcttctgg gaggaacagt agaaggcctg gtgatgtcta aacagtttgg
    10201 catacaggct tttggtgctg tatttactat aatatataca gctatctttt cttatataat
    10261 tttaaaagta attgatatgg taattggact tagagtggac gatgaatctg agctgcaggg
    10321 tcttgaccta gcagagcatg gtgagtctgg ttacgattta taagttttat taatctgaat
    10381 tctaatctca ccagatcaga taatattatc tgatctggtg attaattaac caacatattt
    10441 atttatcagg atctatattt aacagcggta ttgcatcacc acctgtaaac tttggtaata
    10501 caccattcca tttttcagca atcctaagct gaattaaagc ggggttgcct ctcagggctt
    10561 tagcttctct tgctatagca tccgctcttg cttttgaagc tatttctatt tgaaaagcct
    10621 ctgcctctgc tgctagtttt ttctcttccg cggcggcttc tgcctgagtg attcttctta
    10681 tcttttcatt tttagcctgt agcgcttcct gctcagctct aactttcagc tcaatagctt
    10741 tattaaattc gtttgaaaaa tcaaaatctg taatagctac gtttgctata gtgagtgcat
    10801 tttcagcttg tttatccttt agagttattc taataaaatt attgatagct tcttgtattc
    10861 gaattttcac ctcttctcgt ttggtgacta actcttctgc tgtatattga gcggtaattg
    10921 cttttacaga ttcctgtata gcaggttcaa tcaaagtaac tgcgactact tgcctcacgc
    10981 caattttttg atatgttttt ggagctacag cgccaactag agaatactgc acggttacct
    11041 gagttttaac agtttgaagg tccttagaag cagatgccgc ttgcgcatgg gcctttgtaa
    11101 gcctaacatc cattacctca accttatcaa taaatggctt tttaaaatgg aagccttctg
    11161 gaagtgcttt gggctgaact gctccaaatg ttctgacaac tcccacatgt ccactttgaa
    11221 cagtcacata actgttgacc accatcacaa ttatcgcgat aatgaccgga attatgaaat
    11281 taaattttgg tatttttata tcctttaaat cagaagacat tttgaaaacc ccctatagtt
    11341 ttaatttctc tttattagta tcaaagagag aattttaaga attagttatg aatatcaata
    11401 taggcacaat aaataaattt taaagtgcta tgagaataat tgtcataatt tctataaatt
    11461 ttcataaaca atattaccgc caacaatcgt ataattcact tttcctttta ccaccctatc
    11521 atgaaaagga gtgtttttac cctttgacct aaatagatgc ctgtcaatca cagactcatc
    11581 aatagtattg aatattacaa catcagcaac cgatccttct tttaatgtgc ctctatcaga
    11641 accaataatt gcagccggaa tatgtgtgag ctttgaaacc atatcatcaa tagatataac
    11701 tttttgttct actaactgaa gtgacagctg aagtgcggtt tccagtcctg taatcccaaa
    11761 tggcgcctta tcaaactcaa cttttttttc atcctcggaa tgcggggcat ggtctgtggc
    11821 aatagcatct attgtaccgt ctgaaagccc ctcacgtatg gcatcaacat ctttagcagt
    11881 tctgattggc ggatttacct ttgcaaatgt attgtagtcc ttaactattt catcagtaag
    11941 atgaaaatgg tgcggagtag cttctgcagt tactttgact cctcttgatt tagcctctct
    12001 tattattcta actgaacctt cggttgaaac atgacaaaca tgcagccgtg ttccggtaag
    12061 ctcactaaga tttatatctc ttgcgatcat tacctcttca gatgcattgg gtattccttt
    12121 taatccaagt gtagtagaag taaccccttc gttcataacc ccgtcacagg aaagcccctt
    12181 atcctctgca tgaataagga cgggaatatc aaaagccttt acatattcca tggcatttct
    12241 cataatccct gaatgcataa cgggcatacc atcatctgat atccctacac atcctgcttc
    12301 atacatctca ccaatatgcg ctaaggattc accctgctca ccaattgtaa tggcaccaat
    12361 tggaagtacg tttacaatcc cttcagttct ggctttatat aagataaact ctgttactga
    12421 ggcattatca ttaacaggat ttgtattggg catacaaaca atagtcgtaa atcctcctgc
    12481 tgccgcagct tcacaacctg tctttattgt ttccttatgc tcaaaacctg gttctcttaa
    12541 atgaacatgc aggtctatta aacccgggct tattattttt cctttacagt caatcttttt
    12601 aaatcctttg tacttcgatt ctgtaatttt gttgtgaaac ccttttatcc tgccttcctc
    12661 aataagcaca tttccttttt tatttatgtt ctgggatgga tcaataatag ttccattttc
    12721 taggattaga ttcaattttc acctcttttc tgtactacat gatataacat ggacattctt
    12781 accgctatcc cgtttgctac ctgttcgaga attactgatt tagagttatc tgcaagctct
    12841 gagctaagct ctatacctct gttgattggc cccggatgca taagaatcac atcatcattt
    12901 gcaaggttta gtctttgcct tgtaagccca tagaactttg aatactctct taatgatgga
    12961 aagagatttg tattttgacg ttccttttga attctaagca ttataattac atctgctccc
    13021 ttgagggatt gttctaacga ataataatgt ttaacgccaa gttctgagat atatttagga
    13081 attacagttg gtgggccaac cactgagaca ttagctccaa gtttattaaa cccccatatg
    13141 tttgaccttg ctacacgact gtgaagtata tcacccacaa taaccacgtt tagcccttta
    13201 atccttttct ttctttcttt tactgtaaaa aggtcaatca gtgactggct tgggtgttca
    13261 tgcgagccgt ccccggcatt aattatagaa caatcaagtt cttttgccag caaccaaggt
    13321 gcgcctgaca taggatgcct tattacgata atactggcgc ccatagcctt taggttttta
    13381 atcgtatcaa gcagggtttc acccttttgc acactgcttt gatcgaggct taaattaaga
    13441 acatccgcgc tcagtctttt ctccgctatt tcaaatgacg acctggttcg ggtacttggc
    13501 tcaaagaaca gatttactac agtgattcct tttagcgtag gcaccttttt gatatttcta
    13561 gagctaacaa ctttcatgga ttcggcagtc ttaaggatct cattaatatc ctttgccgac
    13621 atctcactta taccaagtat atgtcctttg gaaaaaccca taattatgtt gttgttactt
    13681 caactccttc cctgccgtca gtatcattca aaaagacgtt taccctctta ccccctgcaa
    13741 tatcaaatac tgctccacaa aaatcgggct gaatgggaag ctctctattt cctctgtcta
    13801 caagagtaat gagtttaatg gaagagggtc ttccataatc cattacagct tcaattgcag
    13861 cccttgccgt ccttcccgta tatataacat cgtctacaat tactacatct ttattattaa
    13921 tatcaaaatc aatatcagtc ttttcaatcg taggccatgt atcttttgtt ctgagatcat
    13981 ctctatacaa tgttatatca agtatcccaa gtggtatatt gttaatatta tttgtattga
    14041 gttgttttaa tattctttgt gcaaccacaa tacccctggt tctgatgccc acaatacata
    14101 cattttgaaa atcattaata ctctcttgca gtaattctga tattttggtt attgcagttc
    14161 ttatatcctc tacgtccatc aacaaatctt tattcatatg atgcaagtct tttatttaaa
    14221 caatgtatct gtatcagaaa aacattgatg gtgaaattca aagtcctttt tatatttttt
    14281 ataaaaatgt gcagtatccc aataactgat atccagatat actttttccc cttcgatctt
    14341 tatctgatat ttactatttt gtttaggtat accaattctg cgaagatctt catccactct
    14401 cataaataca aattgactac catatttctt atatctatcg acctgttctg cgcagatacc
    14461 ccttatacca aagactcctt tatatagaat tggtcctact ttaaatgcta caaaaagcag
    14521 tacacataca attataacgg taaatacatt cttagctccc attatcaacc tccaactatt
    14581 gtcatttaat taatttaaat attctttccc atctaacatc actaattaat ttactatttc
    14641 tatcatccca tgaccagtgt gtcagtaaag ccttgcctga aatattttca aatggtacaa
    14701 gaccccaata tcggctgtct ttgctattat ccctgttatc acccatcaca aagtaatgtc
    14761 cttccggtac tttatcaaaa ggtataaaaa tacccttatc agtcgattcc ttgccgttac
    14821 gatatataac tttgtgcttg tggttaaaca gatcctcttc atattcatct acataagtag
    14881 tgtcgt
//
LOCUS       DBNGSV010000085         2473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_154629, whole genome shotgun sequence.
ACCESSION   DBNGSV010000085 DBNGSV010000000
VERSION     DBNGSV010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_154629"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="AC159U_03105"
     CDS             complement(<1..861)
                     /locus_tag="AC159U_03105"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3336074.1"
                     /translation="MSRRVVVTGIGLVTPLGVGNDITWEGICNSRSGVTRIAKFDPSI
                     LRTQIAGQVPKFDPTDFMDPKEARRSDHFIQFACAASKLALDDAGLVIDDELAPRVGT
                     IIGSGIGGMDTYYQTVLTMEEKGPMRVSPFFVPNIVCNMASGYVSIFFNAKGPNSCTT
                     TACAAGAHGVAYAAYSIQNGDADAMICGGTEAPVNAMTIAGFNTMRAISTSNDPPEKA
                     SRPFDKDRDGFIIAEGSGIVILEELEHAKKRGAKIYAEYLGSGMSGDAHHITSPSLDG
                     PQRCMEAALKN"
     gene            complement(861..1106)
                     /gene="acpP"
                     /locus_tag="AC159U_03110"
     CDS             complement(861..1106)
                     /gene="acpP"
                     /locus_tag="AC159U_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3336075.1"
                     /translation="MAVDQELLDNVKKMVAAQLGKDEDEISPESSFIEDLGADSLDLV
                     ELIMSMEDEFGLEISDEDAESIVTVQDAINFITERKK"
     gene            complement(1136..1879)
                     /gene="fabG"
                     /locus_tag="AC159U_03115"
     CDS             complement(1136..1879)
                     /gene="fabG"
                     /locus_tag="AC159U_03115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3336076.1"
                     /translation="MDFKGKVALITGSSRGIGKSVATNLASKGAYVVINYTSNEQAAQ
                     NVKDEIESTGGTCEIRGFDVSSSAQVNEHIDSIINDHGELNFLVNNAGITRDGLLMRM
                     KEEDWDAVINVNLKGVFNCTKAAAKYMIKQKSGRIVNISSVVGEMGNPGQSNYSATKA
                     GIIGFTKSVSRELASRNITVNSITPGFIETDITSELSEKVKEYYLGQIPLGRFGSPED
                     VSGVVSFLLSDEASYITGEVIRVNGGMYT"
     gene            complement(1869..>2473)
                     /locus_tag="AC159U_03120"
     CDS             complement(1869..>2473)
                     /locus_tag="AC159U_03120"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3336077.1"
                     /translation="VRKRGEFTQGAVPVGIGSMAAILGLDKEQIEGICRDASTDGFIV
                     SPANFNAPGQTVISGNNEAVEKAKELAKTQGAKRVVTLDVSAPFHCKMMAPAAEKLGK
                     VLDKIDFSNIRIPLINNWEATLIKDPERLRENLINQVMAPVRWYESIKLIYENLESKK
                     FIEIGPKNVLTNLIRRTLKDVKVVNFDKLADLKALKENGF"
BASE COUNT          689 a          555 c          379 g          850 t
ORIGIN      
        1 atttttaagt gctgcttcca tacatctttg aggtccgtcg agtgaaggtg atgtaatgtg
       61 atgagcatct ccactcattc ctgagccgag atattctgca tatatttttg cacctctttt
      121 ctttgcatgt tcaagctctt ctaaaataac aatccctgag ccctcggcaa tgataaaccc
      181 gtctctgtcc ttatcaaaag gtctggaggc tttttctggt ggatcattgc ttgttgatat
      241 agctctcatt gtgttaaacc ctgcaattgt catagcattt acaggggctt ctgtaccacc
      301 gcaaatcatt gcatctgcat ctccattctg gatagagtaa gcagcatatg caactccgtg
      361 agcacctgct gcgcaagcag ttgttgtaca gctgtttggt cctttagcat taaaaaatat
      421 agacacataa ccagaggcca tattacaaac tatattcgga acaaagaatg gagagactct
      481 cataggtccc ttttcttcca tggttaaaac tgtctgataa tatgtatcca taccaccaat
      541 tcccgagcca attatagttc caacccttgg agccagttca tcatctatta caagccctgc
      601 atcatctaga gcaagctttg atgctgcaca tgcaaactga ataaaatgat cacttcttct
      661 tgcttctttg ggatccatga aatcagtagg gtcaaactta ggcacctgac ctgctatttg
      721 agtccttaat atggatggat caaatttagc tattctggtt actccagaac gactattaca
      781 tattccttcc cacgtaatat cgtttccaac accaagtggg gtaacaagtc caatgcctgt
      841 tactacgacc cttctgctca ttattttttg cgctccgtaa taaaatttat tgcatcctgc
      901 accgtaacaa tactttctgc atcctcatca gatatttcga gtccaaattc atcttccata
      961 gacattatta gttctactag atcgagtgaa tctgctccta aatcttctat aaatgaagat
     1021 tctggtgaaa tctcgtcctc atcttttcca agctgagcgg ccaccatttt ttttacatta
     1081 tcaagtagtt cttgatcaac agccataagt tattcctcct acatacataa taattttaag
     1141 tatacatgcc tccattaacc cttataactt caccagttat ataagaagct tcatctgaca
     1201 ataaaaaact aactacacct gatacatctt ccggactgcc aaatcttcca agtggtattt
     1261 gaccgagata atattcttta accttttcag acaattcact tgtaatatct gtttcaataa
     1321 atccgggtgt aatactatta acagtaatat tacgagatgc aagttctctt gatactgatt
     1381 ttgtaaatcc tataattcct gctttggttg cagagtagtt tgactgaccg gggttaccca
     1441 tttcaccaac cactgagctt atattaacaa tccttccgga tttttgtttt atcatatatt
     1501 tagctgcagc tttggtacag ttaaatactc ctttaagatt aacatttata actgcatccc
     1561 agtcttcttc cttcattctc ataagaagac catctcttgt aatacctgca ttatttacta
     1621 gaaaattcag ctcgccatga tcatttatta tcgagtcaat atgttcatta acctgagcac
     1681 ttgatgaaac atcaaaacct cttatttcac atgtaccacc tgtgctttca atttcatctt
     1741 ttacattttg agcggcctgc tcattggaag tatagttaat aacaacataa gcccctttag
     1801 aagctaagtt ggtagcaacc gattttccaa ttccacgaga tgatccggtt attagagcta
     1861 cttttccttt aaaatccatt ttcttttaat gccttaaggt ctgcaagctt atcaaagttt
     1921 actaccttaa catcttttag tgttcttctt attaaatttg taagcacatt ctttggcccg
     1981 atttctataa acttctttga ctcgagattt tcataaatta gttttataga ttcataccat
     2041 ctaactggag ccataacctg gttaataaga ttttctctaa gtctttcagg atcttttatg
     2101 agcgtggctt cccagttatt aataagtggg attctgatat ttgaaaagtc tattttatct
     2161 aatactttac caagtttttc agcagcaggc gccatcatct tacaatgaaa aggagcgctc
     2221 acatctagtg tgactactct ctttgcaccc tgtgtttttg caagttcttt agctttttct
     2281 acagcttcat tatttccgga aattactgtc tggccaggtg cattgaagtt tgcaggacta
     2341 actataaatc catctgtaga agcatccctg caaattcctt ctatttgttc tttatcaagc
     2401 ccaagaatag cagccataga gcctatacct acaggtactg ccccttgagt aaattctcca
     2461 cgctttctaa cta
//
LOCUS       DBNGSV010000086         4057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_223531, whole genome shotgun sequence.
ACCESSION   DBNGSV010000086 DBNGSV010000000
VERSION     DBNGSV010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_223531"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC159U_03125"
     CDS             <1..592
                     /locus_tag="AC159U_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3336078.1"
                     /translation="GGVIKIDMHFLSDVYVKCDKCSGNRYNDETLQINYKGKTISDIL
                     NMTVHEALDFFINIPKIKKSLQVLSDVGLEYIRLGQQATTLSGGEAQRIKLSKEMSKS
                     SSKNTLYILDEPTIGLHFEDVKKLLTILQELVEKGNSVIVIEHNLDVIKSADYIIDLG
                     PEGGDKGGRVVAKGTPENIIKSKNSYTAKYLKKVLH"
     gene            610..1413
                     /locus_tag="AC159U_03130"
     CDS             610..1413
                     /locus_tag="AC159U_03130"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3336079.1"
                     /translation="MDIFQSIILGIIQGITEFFPISSTAHLALIPWFFSWKDPGLSYN
                     VALHFGSLFAILVYFRNTWKLVISQFIQGALKRNFNDLEYGRVGLFIILATIPAVISG
                     LLFETYAAGILRHPLIIAGSLLIFGLLLLYSNKVSKEKRSINNMTIKDSLIIGAAQAF
                     AIIPGASRSGVTITGALLRNFKTDEAAKFSFLMAAPLIVGATVYESRHMSVESIFQTP
                     FLIGILVSAVFAFLAIKYLLRFVRFRGYKYFVYYRIVLAAVITLMHFLG"
     gene            1447..2163
                     /locus_tag="AC159U_03135"
     CDS             1447..2163
                     /locus_tag="AC159U_03135"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019284 - obsolete L-methionine salvage
                     from S-adenosylmethionine [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA];
                     GO_process: GO:0046124 - purine deoxyribonucleoside
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase family protein"
                     /protein_id="HIR3336080.1"
                     /translation="MTTIITANFKEIEQFISHCKMLKHYSSNSLKIKDIELNSKLYQI
                     IQSGVGIKNARIAVSYAINNLKSEQVYITGVSGALNSELKIGDIIISDWVYSIIKDER
                     INLRYINDKELSGIKTGGLLTHNRFVNTSKAKNELKTKSKAIIVDMETWGAAEVCRKF
                     DIPLTSIRSVSDLASDNLPDFGYIYSSNGSLNTVRSVKYFIRNPYLFYKYLEFRFIKL
                     KKSSCSLSAFLLQIFDKQYT"
     gene            2179..3852
                     /locus_tag="AC159U_03140"
     CDS             2179..3852
                     /locus_tag="AC159U_03140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3336081.1"
                     /translation="MKKLLFTILIIFIFVPIVHANQSRSDTIVLKAMEQELNRSMSKL
                     KLENYDKPYFMSYLVKENTVNQLMAKFGSLIVSESSKTRELFVDVRVGNYQFDNSENG
                     QTGSSQSYKRNNYLPVENDIDGIRAVIWQVTDDTYKKALTQYFTKKAKKVQEIDEDNL
                     PSFSKEKPSTYIGKEKQILFNKKEWETKLKNISSVVKEYKELSDSTVTFTAQKETVFF
                     ISSEKSKYITDDILYSINIDLSAISEDGNVINNFASFHYKDPTDIPDIDTLKKEFRRV
                     IDEAYEIKIAESINPINVPAILEPEAAGILFHEAVGHRLEGERQIDDTQGQTFSERVG
                     TRIIPAFLSIEDNPTLYEFNNESLLGYYLFDDQGIEGQNVTLVKNGILKNFLLSRTPI
                     KGFLNSNGHARSSNSRSPMARMSNLIIKSQNEVQKDELQKLLIEEVKKQKKEFGLIIK
                     KMTGGETNTSTYDFQAFKATPILLHKVDPETGEQTPVRGVEIVGTPLISINKIIATGN
                     DYKVFNGFCGAESGYVPVSTIAPSILISEIELQKTTTEKEKQPILPPPY"
BASE COUNT         1492 a          669 c          723 g         1173 t
ORIGIN      
        1 tgggggagta ataaaaatag atatgcactt cctttcagat gtgtatgtta aatgtgataa
       61 gtgctcaggc aataggtaca acgacgaaac actccagata aactataagg gaaagaccat
      121 ctcagatata ttaaatatga ctgttcatga agctttggat ttttttatta atatcccaaa
      181 aatcaagaaa agcctgcagg tattgtctga tgtaggactt gagtacataa ggcttggaca
      241 acaggccact acactttccg gcggtgaagc acagaggata aagctgtcaa aggaaatgtc
      301 taaaagttct tctaaaaaca cgctttacat actcgatgaa cctacaatcg gactacattt
      361 tgaagatgtg aaaaaactgc ttacaatcct gcaggagctt gtagaaaagg gaaacagtgt
      421 gatcgtgatt gaacataact tagacgtaat taaaagtgct gattacataa ttgatctagg
      481 tcctgaaggt ggagataaag gaggcagggt agtagcaaaa ggaacacctg aaaatattat
      541 taaatctaag aattcttata cggcaaaata cttaaaaaag gtattacact aacacacaaa
      601 ggataaaact tggatatttt tcaatctata attcttggaa taatacaggg cataacagaa
      661 ttctttccta taagcagcac ggctcacctg gcattgatcc cttggttttt ttcttggaaa
      721 gacccggggc taagctacaa cgtagcgctt cactttggaa gcctttttgc aattttggtt
      781 tattttagaa atacatggaa acttgtaatt tcacagttta ttcaaggtgc tcttaaacga
      841 aactttaatg atttggagta tggcagggtt ggcctgttta ttattttagc aactatccca
      901 gctgtaataa gtggactttt atttgaaacc tacgctgccg gaatactcag acacccactc
      961 ataatagcag gttcactgct aatatttggt ctgctattac tttattcaaa taaagtttcc
     1021 aaagaaaaaa gatccataaa caatatgact ataaaagaca gcttaattat aggagctgca
     1081 caggcttttg ccataatacc cggagcttcc agatctggag taacaattac cggtgcactg
     1141 ctaagaaact tcaaaaccga tgaagctgca aaattctctt ttttaatggc agctccgctc
     1201 atagtaggtg ccacagtcta tgaatccaga catatgtcag tggaatctat ttttcagact
     1261 ccttttttaa taggaatatt agtctctgca gtctttgctt ttcttgccat taaatatcta
     1321 cttaggtttg ttagattcag aggctataaa tattttgttt attacagaat cgtacttgct
     1381 gccgtaataa cgttaatgca tttcttaggg tagaatgtac caaactcaaa tatataacca
     1441 taaaaattga ctacaataat aacggcaaac tttaaagaaa ttgaacaatt tataagtcac
     1501 tgcaaaatgt taaaacatta ttcttcaaac agcctcaaaa taaaagatat agaacttaat
     1561 tctaaactct accaaataat acaaagcgga gttgggataa aaaatgccag aatagcagtc
     1621 agctacgcta taaataacct gaaatctgag caagtttata taacaggagt atccggggca
     1681 ttgaattcag aattaaaaat aggagatata ataatctcag attgggttta tagcatcata
     1741 aaggatgaaa ggataaacct tagatacata aatgacaaag agttatcagg aattaaaacc
     1801 ggcggattac tcacacataa caggtttgtt aataccagta aagctaagaa tgaattaaaa
     1861 acgaagtcta aagcaataat tgtagatatg gaaacatggg gagctgctga ggtttgcagg
     1921 aagtttgaca tccctttaac cagcattagg tcagtttccg atctagcttc ggataatctg
     1981 cctgattttg gatatattta tagctctaat ggcagcctga atacagtaag atcggtaaaa
     2041 tattttatta gaaatccata tttgttttat aagtaccttg agtttagatt tattaaatta
     2101 aaaaaatcct cttgcagttt atcagctttt ttattacaaa tatttgataa acagtatacg
     2161 taaactaaaa ggttaaatat gaaaaaacta ctttttacaa tattaattat ttttattttt
     2221 gtacccattg tacatgccaa tcaatctagg tctgacacta tagtcctcaa agcaatggag
     2281 caggaactga ataggtctat gagcaagctt aagcttgaaa attatgataa gccatatttt
     2341 atgagctacc ttgtaaagga aaatacagta aatcagttga tggctaaatt tggatcccta
     2401 atagtttctg aaagcagcaa gacaagagag ctttttgtgg atgtgagagt tggcaattat
     2461 cagttcgata actcagagaa tggtcagact ggaagcagtc agagctacaa gaggaacaat
     2521 tacttacctg ttgaaaacga tattgatggt ataagggctg tgatttggca agtaacagac
     2581 gatacctaca aaaaagctct tacccagtat tttactaaaa aagcaaagaa agtgcaggaa
     2641 atagatgaag ataaccttcc aagcttctca aaggaaaagc caagcactta tataggcaag
     2701 gaaaaacaga ttctttttaa taaaaaagag tgggagacta agcttaaaaa tatttcttct
     2761 gtagtaaaag agtataagga gctcagtgat tctacagtta cctttacggc tcaaaaagaa
     2821 actgttttct ttataagctc tgaaaaatca aaatatatta cagacgacat attgtattct
     2881 attaatatcg atttatcagc catatcagaa gacggaaatg ttataaataa ttttgcaagt
     2941 tttcattaca aagaccccac ggatattcct gatatagata cactaaaaaa agagtttaga
     3001 agagttatag acgaggccta tgaaatcaaa attgctgagt ctattaatcc tatcaatgtt
     3061 cctgcaattt tagaacccga ggctgcagga attttatttc atgaagcagt tggacacaga
     3121 ctagaaggcg agagacagat tgatgataca cagggacaga cattctctga gagagtggga
     3181 acaaggataa ttcctgcctt tctctcaata gaagataacc caaccttata tgaatttaac
     3241 aatgaaagct tactaggata ttatttattt gatgatcagg gaatagaggg tcagaatgta
     3301 acacttgtaa aaaatggtat tcttaaaaac tttcttcttt ctcgcacacc aataaaaggg
     3361 tttttaaatt ctaatggcca cgctaggtca tcaaactcac gctcccctat ggccaggatg
     3421 agcaatctaa taattaaatc tcaaaatgaa gtacaaaaag atgaacttca aaaactatta
     3481 attgaagaag ttaaaaaaca aaaaaaagaa tttggcctta taatcaaaaa aatgacagga
     3541 ggagaaacca atacctctac atatgatttt caggctttta aggcaacccc aatcctgctg
     3601 cataaagtag acccggagac cggagagcaa accccggtaa gaggagttga aattgtaggc
     3661 actccactaa tctcaataaa taaaatcata gcaaccggaa atgattataa agtattcaat
     3721 ggtttttgcg gtgccgaatc cggctatgta cccgtttcaa ccattgctcc aagtatttta
     3781 atttcagaaa ttgagcttca gaaaaccaca acagaaaaag aaaaacaacc cattctccct
     3841 ccaccatatt aaactagttc gatattatat tcacttttta tataataata acgacttatt
     3901 gattttgcat attcgtaata agatattaaa ataattttag gagctcgcat aggacagcaa
     3961 agtatactaa gctgaaacta tgcaaaagaa gttacttaag aatacaaaac tatcaaatag
     4021 taagataagc aagctaatac aaatgtttgt attggaa
//
LOCUS       DBNGSV010000087         9469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1678728, whole genome shotgun sequence.
ACCESSION   DBNGSV010000087 DBNGSV010000000
VERSION     DBNGSV010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1678728"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1432
                     /locus_tag="AC159U_03145"
     CDS             182..1432
                     /locus_tag="AC159U_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR3336082.1"
                     /translation="MAGNSSVMNIFSGKHSLLFGIIIGVLLGGVVGSFFPEFGVKQGF
                     IGEMFLKALKMIVLPLIIVSITLSIMKIGNTGELGSMGLKTILYYASTTAIAVFIGIV
                     VVLIISPGEGGAIITGEMPAKIAGKENFEITSILDYFIHPNLFQAAADFKILPLIVAS
                     ILFGVAFALLGKESEVILRIFEVTEKAVMKIVHWIILFTPVGVFSLIADRIGEAGGGA
                     EVFNIINQLFKYSISVVAGLFVHGFIVLPLILVLIARRNPVDYFAHMSNALLTAFSTA
                     SSSATLPLTMTSVIEKAKVKPKVGRFVLPLGATINMDGTALYEAVAVIFIAQSYGISL
                     GPGDLVIVFFTATLAAIGAAGIPEAGLVTMVLVLQAVGLPLEGVGLILAIDWLLDRFR
                     TTINVWGDSVGCAVIDRLDNKNSS"
     gene            1448..2266
                     /locus_tag="AC159U_03150"
     CDS             1448..2266
                     /locus_tag="AC159U_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3336083.1"
                     /translation="MKKISILGCGWLGLPLGRFLINKGFEVKGSTATPGKVSELKAKG
                     IEPFYISFSPEINTDFDPGFFNSDILIINFPPKRRDDIISYHQDQARSLIGEIKKSII
                     KNIIFVSSTSVYPEVNREVTEDEDLKPTKNSGIALINVEKMLMNIEGIRTTVLRLAGL
                     IGYDRMPGRFLSGKRELKNGNAPVNLIHQDDCILIIYEIIKQKAWGKILNGCCDEHPL
                     RKEYYTNQAKIIGVNPPDFDDSDNANFKIISNKKLKNLLNYSFKYADPSSIKEI"
     gene            complement(2309..2629)
                     /gene="trxA"
                     /locus_tag="AC159U_03155"
     CDS             complement(2309..2629)
                     /gene="trxA"
                     /locus_tag="AC159U_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007552262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3336084.1"
                     /translation="MSIKISSDNFEKEVINSELPVLVDFWAEWCGPCRAVGPVLEELS
                     DEYSDKFRVAKVNVDENPELASEYGIRSIPTMILFKDGKQADSVIGALPKENIVDFVT
                     ENLQ"
     gene            2748..3446
                     /locus_tag="AC159U_03160"
     CDS             2748..3446
                     /locus_tag="AC159U_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3336085.1"
                     /translation="MDIELDVNEIINLDNFSGIAPVFPLSNVVLFPNVLLPLHIFEER
                     YKKMVNDALSGEKLITMCLLKPGWENSYYDRPPVYNAACMGRIVSSELFDDGRSNIVL
                     YGLKRVILDELDNHLPYRTASHTITEDILSRGEELYSKHIEELLLSWNELMGEEQKDH
                     RIDINTGLPLDRLTDTLGTLIFSNVFDRQALLEELDPVKRAEKIIDYIQTRLDILKFT
                     SKIKDNITEVRNLN"
     gene            complement(3453..4238)
                     /locus_tag="AC159U_03165"
     CDS             complement(3453..4238)
                     /locus_tag="AC159U_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3336086.1"
                     /translation="MPIVKANGININYEVHGKGDPVVLIGGLGSHLQSWATQIPIYAQ
                     HFKVIALDNRGMGMSDKPDIEYSVEMMADDTVALVHELGIEKASFVGKSMGGMISQWI
                     GIKYPAVADKLVMGCTTACRDEVGNQIIKTGREIAEKAGMKTVWLTALFLGYSRKYIE
                     DNIKTIKENMSYVTDSKEMLTGYLRQSGACENHDVLDLLPQIKAHTLVMYGENDFIAS
                     PSNSKRLAELIPNAKIIGFNAGHGFWRECQKEVDAQVLDFLLD"
     gene            complement(4225..4650)
                     /gene="ruvX"
                     /locus_tag="AC159U_03170"
     CDS             complement(4225..4650)
                     /gene="ruvX"
                     /locus_tag="AC159U_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR3336087.1"
                     /translation="MSLDVGTKRIGIAVSDELGITANGLQTLKRTDNKSDIKKLKNLC
                     SELNVEKVIVGIPYNTDGQISKNGQVIKNFSEKLRKELSLPVDYINEAFTTMDAQQYL
                     LEANMSRKKRKKVIDKLSAVIMLQEYLEQMRENPKNADS"
     gene            4885..5370
                     /locus_tag="AC159U_03175"
     CDS             4885..5370
                     /locus_tag="AC159U_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336088.1"
                     /translation="MKYCGNLGKALSLILITLFGAGTAVVHILDQSESKILDNTGVQS
                     SYSSKPDIEVQTYRKQLRNSYNLEDTQSNAGISGKNKSLWSGPHYTQDIRLNSNLRPE
                     IVEISKNNSFEQLNNEKKFWYSQYNILIKQKNNKQAAKDAYNKYRKYKEAINIKRAYN
                     R"
     gene            5508..6197
                     /locus_tag="AC159U_03180"
     CDS             5508..6197
                     /locus_tag="AC159U_03180"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3336089.1"
                     /translation="MGLPAISITLNAKEVDRKVLDQEIIAIGRDMDNDIVINNLAVSR
                     CHAILTVLDDKIIVKDMASSNGTFINGKKIEEGVLNAGDLMLIGKHILRLEIDEEPQA
                     VQPEENKEAEEGTVIFDSDTRDKFLEKMQTEKAASDPRLVVNGEEEIKIKGDFFAIGK
                     RYDADINISGLFINEHHAIIQKQKEGTYKIINMGSFLKPTKVNDSVIKEKILQKGDVI
                     QIGNYRIVFSM"
     gene            6211..7041
                     /locus_tag="AC159U_03185"
     CDS             6211..7041
                     /locus_tag="AC159U_03185"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR3336090.1"
                     /translation="MHEPTLFISYGNSDVGRVRTNNEDSYLYKTFSLNSNGTPDAFLM
                     VVADGMGGHAAGEVASAKVIEELNDNLNEKSLEKLPYILKEIIDEANSKIFNISAQNT
                     ELNGMGTTCTAMIYYKEQVHLAHVGDSRAYLIRDGEISRLSKDHTVVEELLEAGTITE
                     EKARTIPERNILLRAVGTSEQLEVDVYPPMDTSGGDIFLLCSDGLTEYLYEEEIRDTV
                     TSVPLENVCDELIKIANERGGADNITVQLAILREKPKIKKTRQILKIFKNILFDESTT
                     "
     gene            7055..9370
                     /locus_tag="AC159U_03190"
     CDS             7055..9370
                     /locus_tag="AC159U_03190"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3336091.1"
                     /translation="MSELLGQKIENYKILESIGVGGMGEVYKAIHETLDRVVALKVIH
                     PELLSNADIIKRFYKEAKIQAQMTHPNIVTVFDFLEVQNSYFLVMEYIEGESIGKIIK
                     KQGPQEINFALKIFKQILSGISHAHSKGIIHRDIKPNNFILTKGEVKITDFGIAHIVS
                     DSVHTVAGTVLGTPKYMSPEQILGEIVDHRSDIYSLGVSFYEILAGKVPFGSNSGSDY
                     EIKKGHVELTPTPISEIRPDVSKDLENAIARSMAKRPEARFQSVDDFLAALKIGVSSD
                     LSIRIDGISSSDTTEPTIYTGETTTANQVSFEDLDEYDESGDIEKRFFSQILCLFYRE
                     KKTGILYISAQTELKIYFLQGNIVFLHGSNQRYALAELLVNKSIITKEDQRAAIAFAN
                     ERDIKIGEALIKMGKINPHQLSTVLEDQIRKRLMEGFGLSKGKFYYTDTNLLKLEVMY
                     NINPLQVIYDAVEKSIVNDTEISNLLNNNNTIISRRWGLAQDIATLKFSSTKELKLAN
                     SIKKAKTIGELISISPLDEENTKRFILFLHTAEFIDLRDESSEEEQLEDIIITNESSA
                     SEPDFDEEIEELELEIIDDSQFDNLELSDDKVSSEPIEQSTENNNDFKIEINFPGTKQ
                     QQEKTPEEGAKVDEQIVDLEDNISGEKTKVLSSDEIEKIRAELSGSNKKDSTESVSDF
                     KLDFDPDKFEANSPNSNVKQQADPNEINLEIELPRLGGDLKSQLKTPDNKSASTNKGH
                     SESQRKMSDEHTQELKQEEIEELRKLTIRKN"
BASE COUNT         3203 a         1580 c         1818 g         2868 t
ORIGIN      
        1 aaacactttg ttttgtatct aaaagagatg gaatttagat ttaataacag aaatgtagat
       61 ttagttaata aaataaagga gattctgcat tacttatagc tctgttctat gcgagctcct
      121 taatttaaat tgtattataa tactttttca atatcgtcat taagaacagg agaatacata
      181 tatggcaggc aatagcagtg ttatgaatat attttcagga aaacattctt tactctttgg
      241 aataattatt ggtgtcctgc tagggggtgt agttggctca tttttcccgg aattcggggt
      301 caaacaggga tttataggtg agatgttttt aaaagcgctt aagatgatag tgcttccact
      361 gataatagtt tcgataacac taagcataat gaagatcgga aacacaggtg agctcggctc
      421 catgggtctt aaaactattc tctactatgc atccacaacg gccatagctg tatttatagg
      481 aattgtcgta gtcctgataa tttctccagg tgaaggaggg gcaattatca caggcgaaat
      541 gccagcaaaa attgcaggta aggaaaattt cgaaataaca tctattttgg actattttat
      601 acacccaaac ctgttccagg cagccgcaga ttttaagata ctgccgctca ttgtggcatc
      661 tatactattc ggtgtggcgt ttgccttact gggtaaagag agtgaagtga tactcaggat
      721 ttttgaagtc accgaaaaag ccgtgatgaa aatagtacac tggataatac tctttacacc
      781 agtgggagtt tttagcctta ttgcggacag gattggagaa gccggcggcg gtgctgaagt
      841 atttaatatt ataaatcagc tatttaaata tagtattagc gttgtggcgg ggttgtttgt
      901 acacgggttt atagttcttc cgttgattct tgtactaatt gccagaagaa accctgtgga
      961 ctattttgct catatgagca atgctttgct tacagcattt tcaaccgcat cttcgtcagc
     1021 gactctgcct cttacaatga cctccgtaat tgaaaaagca aaagtgaaac caaaggtagg
     1081 caggtttgtg cttccccttg gagccacaat aaacatggat ggcaccgctt tatatgaagc
     1141 agtagcagtt atttttattg cacagagcta cggtatttcg ctcgggccgg gtgatcttgt
     1201 gattgtgttt tttactgcca ctcttgctgc aattggggca gcaggaatcc ctgaggcggg
     1261 gcttgtgaca atggtgcttg ttcttcaggc agtcggactt ccgcttgaag gggtcgggtt
     1321 aattctagcc attgactggc tgctcgacag attcagaact actattaacg tatggggaga
     1381 tagtgtagga tgtgccgtta ttgacaggct tgataataaa aacagttctt aattcggatt
     1441 ataaattgtg aaaaaaataa gtattctagg ttgtggctgg ctcggtcttc cactcgggcg
     1501 gtttcttata aataaaggat ttgaggtaaa aggatcaacg gcaacacccg gtaaggtatc
     1561 agaattaaaa gcaaaaggca tagaaccgtt ttatattagc ttctctcctg aaattaatac
     1621 agactttgac ccgggatttt ttaatagtga tatattaata ataaactttc ctccaaaaag
     1681 aagagatgac attatttcct atcatcagga tcaggccaga tcacttatag gtgaaattaa
     1741 aaaatcaatt attaaaaata taatttttgt aagttctact tcagtttacc ccgaagttaa
     1801 cagggaagtt acagaggatg aagaccttaa gcccacaaaa aattcaggca tagcattaat
     1861 taatgtagaa aaaatgctta tgaatattga gggtattaga acaactgtgt taagacttgc
     1921 agggcttatt ggttatgaca gaatgccggg caggtttcta tcgggcaaaa gagagctaaa
     1981 aaacggtaat gctcctgtaa accttataca tcaggatgac tgtatattga taatttatga
     2041 aataatcaaa caaaaagcat ggggtaagat attaaacgga tgctgtgatg aacatccttt
     2101 aaggaaagag tactatacta atcaggcaaa gattattggg gtaaatcctc ctgattttga
     2161 cgattcggac aatgcaaatt ttaaaatcat aagtaataaa aaactcaaaa atcttctgaa
     2221 ttatagcttt aagtatgccg atccttcttc aattaaagaa atttaggagg ggattgtgtt
     2281 gctcccctcc ttttcatttt aactgatttc attgtagatt ctctgtaaca aagtcaacaa
     2341 tgttttcctt tggcagtgct cctattacac tgtctgcctg ctttccgtct ttaaatagaa
     2401 tcatggtcgg aatgcttctt atgccatact cagaagcaag ctctggattt tcatccacgt
     2461 ttaccttggc aactctaaac ttgtctgaat attcatcaga cagttcttca agcactggcc
     2521 caacagccct acagggcccg caccactcag cccaaaagtc taccagtact ggtagctctg
     2581 agtttatgac ttctttctca aagttgtcac tgcttatttt tatgctcata tactgcctcc
     2641 tgtgtcatta tttgcctatt tagacatgta tctatataaa aggattcaat aattataaaa
     2701 aataaaattt ttgaactttg taataatgtt ataaacttta tattagtatg gatattgagc
     2761 tcgatgtaaa tgaaataata aatctggata acttttcagg gattgcacct gtatttcctt
     2821 tatctaatgt agtgttattt ccaaatgttc tccttccgct gcatatattt gaagagaggt
     2881 acaagaaaat ggttaatgat gctctaagtg gagaaaaatt aatcacaatg tgtcttctta
     2941 aaccaggttg ggaaaatagt tattacgaca ggccgcctgt atataatgct gcctgcatgg
     3001 gaagaattgt aagctccgag ttatttgatg atggaaggtc caatatcgtg ttatatggct
     3061 tgaagagggt tattttagat gagcttgata atcatttacc atacagaaca gcaagtcata
     3121 caataacaga agatatacta agcagaggcg aggagcttta tagcaaacat atagaagagt
     3181 tgctattgag ttggaacgaa cttatgggtg aagaacaaaa agatcataga attgatataa
     3241 atacaggact tcctcttgat cgtttaacag atactctggg tactctaata ttttcaaatg
     3301 tctttgatag acaggccttg ctggaagaac tagatccggt taagagggca gaaaaaataa
     3361 ttgattatat acagacaagg cttgatatac ttaagtttac atcaaaaatt aaagacaata
     3421 ttacagaggt cagaaatctc aactgaccga atttaatcaa gcaagaaatc cagtacttga
     3481 gcatctactt ctttctgaca ttctctccaa aagccgtgcc cggcgttaaa acctataatt
     3541 tttgcatttg ggattaattc tgcaagtctt tttgaattag aaggagaagc tataaaatca
     3601 ttctctccat acattacaag agtatgggct tttatttgcg ggagaagatc caacacatca
     3661 tgattttcgc aagctccact ttgtctcagg tacccggtta acatttcttt agaatctgta
     3721 acatatgaca tattctcttt gatagttttt atattatctt ctatatactt tcttgaataa
     3781 cctaaaaaaa gtgctgtaag ccaaactgtt ttcatccctg ccttttctgc aatttctctt
     3841 cctgttttta taatctgatt tcctacttca tccctgcatg ctgttgtgca acccattact
     3901 agtttgtctg ccactgcggg atatttgatc ccaatccatt gcgatatcat ccctcccatt
     3961 gatttaccaa caaaactggc tttttcgata ccaagctcat gaacaagagc tacagtgtca
     4021 tctgccatca tttctaccga gtattctata tcgggcttat ccgacattcc catacctctg
     4081 ttatcaagag caattacctt aaaatgttga gcgtatatag ggatttgtgt agcccagctt
     4141 tgaaggtggc ttccaagtcc tccaatcagg acaaccggat cacctttgcc atgaacttca
     4201 taatttatat taattccatt tgctttaact atcggcattt tttgggttct ctctcatttg
     4261 ctctaggtat tcctgcagca tgattactgc agacagcttg tctatgactt tttttctttt
     4321 ttttctgctc atatttgctt ccagtaaata ttgctgtgca tccatggttg tgaatgcttc
     4381 atttatataa tcaactggca gggacagctc ttttctaagc ttttctgaga aattttttat
     4441 tacttgcccg tttttactga tctgtccatc tgtattataa gggatcccta cgattacctt
     4501 ttcaacattt aactcggaac aaagattttt taatttttta atgtcactct tgttatcagt
     4561 cctctttaaa gtttgtagcc cgtttgctgt tattccaagc tcatcactca ccgctattcc
     4621 tattcttttt gtacctacat caagtgacat tatcttcatg aatgttttat tatataagaa
     4681 aacaggaaaa tatgtcaaaa aagcgcaatt ttgacattat ctgacaaaaa ttgtattttc
     4741 aaaagttaat ttggggtttt aaatacaagg gtaaaaaaat aattcagcaa ttacaggtag
     4801 ttgtatttcg agagtaatgg catggaattt tctttattta tttagaagaa tgaaggcaaa
     4861 ttacggtttt agtatgtcta ttggatgaaa tattgtggaa acttaggaaa ggcattaagt
     4921 ttaattttaa taacactctt tggcgcaggt acagcagtag tacatatact tgatcaatct
     4981 gaatccaaga ttttagataa taccggagta caaagcagtt attcttccaa gccggatata
     5041 gaagtacaaa catacaggaa acaacttcgt aacagctaca atcttgaaga cacgcaaagt
     5101 aatgccggca ttagtggtaa gaataaatcc ttatggtcgg gaccgcatta tacgcaagat
     5161 atcagattaa attccaattt aagaccagag attgtagaaa tttccaaaaa caattcgttt
     5221 gagcagttaa acaatgaaaa gaaattctgg tacagccagt ataatatatt aatcaaacag
     5281 aaaaataata aacaagctgc aaaagacgct tataacaaat atagaaagta taaagaagct
     5341 atcaatatta aaagagcata caacaggtaa attactcaaa ttttcctgct tcttttgttt
     5401 atatacccta aattacaatt tagttttaat attcaatccc ctgtagtatt ctataagttt
     5461 ttgctttata atctattcaa aaaacttaat aaacagagga ttattatatg ggtttaccag
     5521 caataagtat tacattaaat gcaaaggaag ttgatagaaa agtcctggat caggaaatca
     5581 tcgcaattgg cagagatatg gacaatgaca ttgtaataaa caatctggcc gtttcaaggt
     5641 gtcatgcaat tctaactgta cttgatgaca agataatagt aaaagatatg gcaagctcta
     5701 atgggacatt tataaatggt aagaagatag aagaaggtgt tctaaatgcc ggtgacctga
     5761 tgcttatagg aaaacatatt ctcaggctcg aaattgatga agagccacag gccgtacaac
     5821 ccgaggaaaa caaagaagca gaagaaggta cagtaatatt tgattcagat accagggata
     5881 aatttttaga aaagatgcag actgaaaaag cggcatccga tccaaggctt gtcgtaaatg
     5941 gtgaagagga aattaaaatc aaaggggatt tctttgcaat tgggaaaaga tatgatgcgg
     6001 acattaatat tagcggacta ttcattaatg aacatcatgc aattatacag aagcaaaaag
     6061 aaggaacata caagattata aacatgggtt catttttaaa accaaccaaa gtaaatgata
     6121 gtgttattaa agagaaaata ttgcagaaag gagatgtaat acagattggg aattacagga
     6181 ttgttttttc tatgtaaaaa ctaaatagtt atgcatgaac ctactttgtt catttcgtat
     6241 ggaaatagtg atgttggaag agtcaggact aataatgaag attcatatct atacaaaact
     6301 ttttcactga atagtaatgg tactccggat gcttttttga tggttgtggc cgatggtatg
     6361 ggcggtcatg cagcaggtga agtcgccagt gccaaagtaa tcgaagaact caacgacaat
     6421 ttaaatgaaa agtcgttaga gaaactgccc tatatcctaa aagaaataat cgatgaagcc
     6481 aattcaaaaa tcttcaatat atctgcacaa aatactgagc taaacggaat ggggacaact
     6541 tgcactgcca tgatatatta taaagagcag gttcatttag cacatgtggg tgacagcagg
     6601 gcgtatctaa taagagatgg agagataagc cgattatcga aggaccatac agtggtagag
     6661 gagcttcttg aagccggcac tatcaccgag gaaaaagcaa gaactattcc tgagagaaat
     6721 attctgctta gggccgtggg aacatccgaa cagttagagg ttgatgttta tccgcctatg
     6781 gatacaagcg gtggagacat ttttcttctt tgctccgacg ggcttaccga atatctttat
     6841 gaagaagaaa taagggatac agttacatct gttcctcttg aaaatgtttg tgatgaatta
     6901 ataaaaatcg caaatgaaag gggaggggct gataacatta cagtgcagct tgcaatactt
     6961 agggaaaaac caaaaattaa gaaaactcgt cagatactta aaatatttaa aaatatatta
     7021 tttgacgaat caacaacatg attaattaac tgctatgtct gaactacttg gtcagaaaat
     7081 tgaaaattat aagattttag agagtatcgg agttgggggt atgggagagg tgtataaggc
     7141 catacatgag actctggaca gagtagtcgc gcttaaggtt attcatcctg aacttctcag
     7201 caatgctgac ataataaaaa gattttataa agaagcaaaa attcaggcac agatgactca
     7261 cccaaatatt gtaactgttt ttgattttct cgaggttcag aacagctact ttttagtcat
     7321 ggaatatata gaaggggaaa gcataggaaa aatcattaaa aaacaaggtc ctcaggaaat
     7381 caacttcgcg cttaagatat tcaagcagat tctttcagga atttcccatg ctcattccaa
     7441 gggcataatt cacagagata taaaaccaaa taactttatt cttactaaag gcgaagttaa
     7501 aatcactgat tttggtattg ctcatattgt ttccgattcg gtccatacag ttgccggcac
     7561 tgttcttggt acacctaaat atatgtctcc cgaacagatt cttggagaaa tagttgatca
     7621 cagatccgat atatattctc ttggagtttc attttatgaa atactggcag gaaaggttcc
     7681 atttggaagc aattcaggat ctgattatga aataaaaaaa gggcatgttg agcttactcc
     7741 aacgcctatt agtgaaatca gaccagatgt atctaaagat ttagaaaatg ctattgctag
     7801 atcaatggca aaaagacccg aagcgcggtt tcagtcagtt gatgattttc ttgcagcact
     7861 gaaaatagga gtttcatctg atctgtcaat cagaattgat ggaataagta gttctgatac
     7921 tacagagcct actatatata ccggagaaac tactaccgcc aaccaggtta gctttgaaga
     7981 cttagatgaa tatgatgaaa gcggggatat agaaaaacgg tttttttctc aaattctgtg
     8041 tttattctac agggaaaaaa agacggggat tttgtatata tctgcacaaa cagaacttaa
     8101 aatctatttt cttcagggaa acatagtttt tctacacggc agcaaccagc gttatgctct
     8161 tgctgagctt ttagtaaaca aatctataat taccaaagaa gatcagcggg cagccattgc
     8221 ctttgctaat gaaagggata ttaaaattgg tgaagccctt attaaaatgg gaaaaataaa
     8281 ccctcaccaa ctttctactg tacttgaaga tcaaataaga aagagattga tggagggttt
     8341 cgggctcagt aaaggtaaat tctattatac agacacaaac ctgttgaagt tggaggttat
     8401 gtacaatatt aaccccctgc aggtgattta tgacgcagtt gaaaaatcaa tagttaatga
     8461 tactgaaata tcaaatttac ttaacaataa taatactatt attagtagaa gatggggttt
     8521 agcacaggat attgctaccc ttaaattttc atctacaaaa gaattaaagc ttgcaaattc
     8581 tattaaaaaa gcaaaaacaa taggtgaact aatttcaatt agcccccttg atgaagaaaa
     8641 tacgaaaagg ttcattcttt ttcttcatac tgcagaattt attgatctaa gagatgaatc
     8701 ttctgaagaa gaacagttag aggatataat cataacaaat gaatcctcag catctgaacc
     8761 agattttgac gaagaaatag aagagctgga gctcgaaata atagacgatt cccaatttga
     8821 taacttagag ctaagtgatg ataaagttag tagtgaacca atagaacaat ctactgaaaa
     8881 taacaatgat tttaaaatag aaataaattt ccccggtacc aaacagcagc aggaaaagac
     8941 acccgaagaa ggtgcaaaag ttgatgaaca gatagtagac ttagaggata atattagtgg
     9001 ggaaaaaact aaggtgctct caagcgatga aattgaaaaa attcgcgctg aattgtccgg
     9061 ttcaaataaa aaagacagta ctgaaagtgt ttcagatttt aaacttgatt ttgatcctga
     9121 taaatttgaa gcaaatagcc caaatagcaa tgttaaacag caagcagatc ccaatgaaat
     9181 aaatctggaa attgaactgc cgcgactcgg tggtgatttg aaaagtcagc taaaaacacc
     9241 tgataacaaa agcgcttcta caaataaagg ccactcagaa tctcagagga aaatgagtga
     9301 tgagcacact caggaactga aacaggaaga aatagaagag ttgaggaagc ttactataag
     9361 gaagaactaa ggctttattc tagtaaaaat accaaaacat aaggtttctc aaaagattaa
     9421 tctcagaaat attaagtata acattaataa aataaaccct tcttttttt
//
LOCUS       DBNGSV010000088         3145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1193923, whole genome shotgun sequence.
ACCESSION   DBNGSV010000088 DBNGSV010000000
VERSION     DBNGSV010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1193923"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..790)
                     /locus_tag="AC159U_03195"
     CDS             complement(131..790)
                     /locus_tag="AC159U_03195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3336092.1"
                     /translation="MQLSINSEAPDFTAETTEGTINFHQWIGDSWAIIFSHPKDFTPV
                     CTTELGTMAGLVPEFAKRNCKVIGLSMDPVDSHKEWSKDIEAAEGNPVTFPMIGDTNL
                     EVAKLYNMLPAEESGTTEGRTPANNATVRSVFIIGPDKKIKLMLTYPMTTGRNFDEIL
                     RVLDSMQLTAKHKVATPANWKDGDDVIIVPAVSDEDAKEMFPDGWNSVNPYIRKVKQP
                     R"
     gene            complement(935..1636)
                     /locus_tag="AC159U_03200"
     CDS             complement(935..1636)
                     /locus_tag="AC159U_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3336093.1"
                     /translation="MRKNMFLICLISTFLFLGCVHGGMKIVDPVQVSPQNYKVLLDNE
                     YVRILEITAKSGEKDEMHSHPASAWYAVSSSKIRLHDTDGSSKDIDIEAGTSNFQDAV
                     AMHSMENIGNNDVKIVMVELKDKKPPVLEEKGPDPLKVSPDVYRLLSENDTVRILELT
                     TKPGQKDNMHGHPANVFYVLSGQSGKLYDEQGEVIELTISAGTASYQNATQNHQFENS
                     GDTEIKVIMFELKSK"
     gene            complement(2461..2727)
                     /locus_tag="AC159U_03205"
     CDS             complement(2461..2727)
                     /locus_tag="AC159U_03205"
                     /inference="COORDINATES: protein motif:HMM:NF033662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336094.1"
                     /translation="MGSKNKNFTILFFLFFSLILISCNSGDINIDSSGIAQEANCTDG
                     LDNDGDGLTDCADLNCAFRPVCIDDGGVAGEPIVRTVLIMMGIT"
BASE COUNT          938 a          651 c          523 g         1033 t
ORIGIN      
        1 tccaatacaa acatttgtat tagcttgctt atcttactat ttgatagttt tgtattctta
       61 agtaacttct tttgcatagt ttcagcttag tatactttgc tgtcctatgc gagctcctaa
      121 attaatatta ttatctaggc tgttttactt ttctgatata aggattaaca gagttccatc
      181 catcaggaaa catttccttt gcatcttcgt cagatacagc gggaacgata atcacatcat
      241 cgccgtcctt ccagtttgca ggtgtagcaa ctttgtgttt ggctgtaagc tgcattgagt
      301 ccagaactct taagatttcg tcaaaatttc ttcctgttgt cataggataa gtaagcataa
      361 gttttatttt cttatccggg cctatgataa atacagacct aactgttgca ttattagcag
      421 gtgttctgcc ttcagtagtg ccggattctt ctgcaggaag catattgtag agctttgcaa
      481 cctcaaggtt cgtatctcca atcataggga aggtaacagg atttccctcg gcagcctcaa
      541 tatcttttga ccactctttg tgagaatcca cgggatccat acttagtcca attaccttgc
      601 agttgcgttt agcaaattca ggaacaagcc ctgccattgt gccaagctca gttgtacata
      661 ccggtgtgaa gtcttttgga tgtgaaaaaa ttatagccca gctatcccca atccattggt
      721 gaaaattgat agtcccctct gttgtctcgg ctgtaaaatc aggagcttct gaattaatac
      781 ttaattgcat tttaaatacc tcctaacaaa tttttttagt taattatatt ttaagatgat
      841 aatattctaa ttagtattca tatacctata tctagaatac atagagtatt ataaatgaca
      901 atgaaaatac gggtagccgg tttttattca cctgttattt actttttaac tcaaacatta
      961 tgacttttat ttcggtatca ccggaatttt caaactggtg attttgcgta gcattctgat
     1021 agcttgctgt acccgctgat atggttaact caattacttc accttgttca tcatataatt
     1081 tgccggattg tccagagagc acatagaata catttgccgg gtgtccgtgc atattatcct
     1141 tttgccctgg tttggttgta agctctaaga ttcttactgt gtcattttcc gataacaatc
     1201 tatatacatc aggcgatact tttaaaggat ccggtccttt ttcttcaagc acaggcggct
     1261 ttttgtcctt tagctcaacc attacaatct ttacatcatt atttccaata ttctccattg
     1321 aatgcattgc aacagcatcc tgaaaatttg atgtacccgc ctcaatatca atgtcttttg
     1381 aacttccgtc tgtatcatga agccttattt tagaacttga aactgcgtac caggcacttg
     1441 cagggtggga atgcatttca tccttttctc cagacttcgc tgtaatctcc aatatgcgga
     1501 cgtattcatt atcgagtaat actttatagt tttggggcga tacttgcacg ggatcaacaa
     1561 ttttcattcc accatgcaca caccctagaa ataaaaaagt actaatcaaa catattaaaa
     1621 acatattttt tctcatgtct tctctcctat aagagcattt catttctact ccttcagttc
     1681 agaaatagtt aatgcttaat gtgcaatttt attacttaat gggatgaaaa gcaatcagaa
     1741 aggtttcaaa ccgaactcat atttatcaaa taatactaga tttttacagt ggacgaacta
     1801 tgataataga agtgtcggat agtgtggcaa tcgattgagt ttttatgtaa tatttctaat
     1861 agtttcttac aaaagcaccc cggccaatac aactgtttgg ccggagtttt cccttctgta
     1921 gcaaaaaaca tgtttcgatt cttactacgt atcctatatc gttaagctta caacagatac
     1981 caatgactat ttagaacaaa ccgtctccgc aatcgacaaa gaaaaatgac agcggcgcgc
     2041 atctatctac cacaccagtg cattcaaatt cattcatatc accatcaaag aatccatcat
     2101 caaagatatc atctaaacca tcgaaacatt cactgcataa tagatcatcc gggcagtccg
     2161 catttgaagt gcaaacatca caagcgcctt caggaacgca tgagtcaggt agtattgtgt
     2221 cttctgttaa tgttacacaa tcaaagttgt ctaaatctgc gatacaatca tcaggatctg
     2281 gtaaattttc tacagtttca caatcagtga aaaatgtaaa aaacgataca caattcccaa
     2341 aagcagattg ttcattacat tctccgaaac gatcacacag ggcatctata aaatcttcgc
     2401 agtcaccaac ggcagcatca catgcagggt ctgataggca atttagatct gtgcaatcta
     2461 tcaggttatc cccatcatta tcaataccgt ccgtacaatt ggttctcctg ctacccctcc
     2521 atcatctatg catacgggtc tgaatgcaca atttagatct gcgcaatctg tcagtccatc
     2581 cccgtcgtta tcaagaccgt ccgtacaatt agcctcttgt gctattcctg agctgtctat
     2641 attgatatcc ccgctattgc acgaaattaa aatgagggaa aagaacagga aaaacaagat
     2701 ggtaaagttt ttatttttac ttcccattat acacccccta caaagatatt aatcttctac
     2761 cacaacctca tcttctactt aatataggtg catatctcat gacagaagag atagaaaaac
     2821 atactaatat ttacagttag ttagggggaa atagacttaa ttagatgtat tgtgtcttaa
     2881 aaacttacat ggctggaaga catgatatta ttgaactgaa aagcttgaat tatattcaag
     2941 tacaccccga ccattacaac tgattggccg gggctttgtt tctaattaga aatgatacag
     3001 taatgatccg aaaagacgat tgtcaagccc cagaattcag gtccaccttt agagttagaa
     3061 tttgctgata ttttgtcatt gagttgaagt acatgtttgc tcatagtttt tggatttatt
     3121 atctgatata acagctcgtg gagca
//
LOCUS       DBNGSV010000089         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_570401, whole genome shotgun sequence.
ACCESSION   DBNGSV010000089 DBNGSV010000000
VERSION     DBNGSV010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_570401"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC159U_03210"
     CDS             complement(<1..462)
                     /locus_tag="AC159U_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336095.1"
                     /translation="MNSIKSIKTFLIFFIMSLVLFSTTQLLSASNKTNEKTYNTARKE
                     FLKFTPDGFNKSIELYNNLIKSDPGYAKAYSGISEVYSYLANYKVHIKEDYEEPFNKS
                     YINLKKALKLSPNSLETKRALATNYLHLRWNTRAKKVANSLISKYPSSPEAY"
     gene            complement(475..1008)
                     /locus_tag="AC159U_03215"
     CDS             complement(475..1008)
                     /locus_tag="AC159U_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336096.1"
                     /translation="MKHNYKIILLAILSVFVLNCGTLGKLDTSSYYSKPETLLRSYTA
                     FEITDFESYDETIPLILKTQIPGEVENQLIQSNIGFQTINYGPIDNVPASETIIMLAE
                     IVDVQSSKDFKFDKGALKFGESSISIKIALVQKDNGREILTAEVAGFSSLGFGKSADK
                     FKDKIANEIVEVIAANY"
     gene            2088..>2342
                     /locus_tag="AC159U_03220"
     CDS             2088..>2342
                     /locus_tag="AC159U_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR3336097.1"
                     /translation="MPKLIKRKKGQKGFTLIELLVVAAIIGILLALAIPNLIKARMSA
                     NEANARKSMQTLRDAEAEYFEQDLDGNNTRDYTQLIGSLTT"
BASE COUNT          773 a          328 c          394 g          847 t
ORIGIN      
        1 atatgcctcg ggactcgatg ggtattttga tataagcgag tttgcaacct tttttgccct
       61 ggtgttccat ctcaggtgca gatagtttgt ggcaagtgca cgtttggttt caagtgagtt
      121 aggggagagt ttaagagcct tttttaggtt tatataagac ttgttgaatg gttcttcata
      181 atcttctttt atatggactt tatagttggc aagatacgaa taaacttctg atatcccgct
      241 ataggccttg gcatagcccg ggtcggattt tataagatta ttgtatagtt cgatagactt
      301 attaaaacca tcaggggtaa attttaaaaa ttcctttcta gctgtattat aagtcttttc
      361 atttgtctta ttcgatgcag atagtaattg cgtagtactg aatagtacca atgacataat
      421 aaagaatatc agaaatgtct ttattgattt aatactgttc atcaatgtat cctcctaata
      481 gtttgctgct attacttcca ctatttcatt tgctatttta tctttgaatt tatcggccga
      541 ttttccaaat ccaagggaac tgaaccccgc tacttctgcc gtgagtatct ccctgccgtt
      601 atctttttgt acaagcgcaa tctttatgct tattgatgat tctccaaatt taagcgctcc
      661 cttatcaaac ttgaaatcct ttgaagactg aacatctacg atttcagcaa gcatgataat
      721 ggtttcacta gcgggtacat tatctatagg cccgtaatta atagtttgaa aacctatatt
      781 agattgtatt aattgattct ccacctcacc gggtatctgt gtttttaaaa ttaaaggaat
      841 cgtttcatca taggattcaa aatcggtaat ttcaaaggcc gtgtagcttc tcagaagtgt
      901 ctctggcttg ctataataag aggatgtatc cagtttgcct aaagtgccgc agttgagtac
      961 aaaaacgctt agtatggcta ataatataat cttgtaatta tgtttcattt attgctctcc
     1021 attgagaatg gtaaatttag tattataaca taacaaccac gaggaatcac acatcaatgt
     1081 aattataata aaccactgtt aaggtagata gataattttt attgtaaata tcaaataacg
     1141 atgaacttta cttgtagatt atccgattta gacagaaaat aatgaaaaac tgggtaattt
     1201 tggaaaattt ctatatgtat atggattcaa ttatatacaa aaaaaataat cagattattt
     1261 aatctgatca gatcattaat tataaataaa taagtagcta aataggcaaa aatttggatg
     1321 aaagcataaa agctatgtat ttgaaaaaag tatattgtta tgctaattag cctcgctcgg
     1381 ttaagtgtat ggctgtttaa taataaatag agtattgcta atacaggatt attacaggtt
     1441 taatcaattt atattaatta ttgctatttt atgtgctgta tttaaatatt gagaggtgtc
     1501 ataagaatct gtataagtat gtatgttcaa tatatacagg gtttaatgta tgtaataata
     1561 aatgtacttc tcttctttcg atattcgaaa tacatcttta tatctttttt attatatatg
     1621 caggttatta ggggaaaaat atcctaatat gacgaaaatt attactgctt gagcaaattg
     1681 tctggatctt ttgattgaca aatattgtta cagtatttta catgtaataa gtcttgtgaa
     1741 attgtataaa ttatacaagg tataatatgt gaacataata atatctatat atagttatta
     1801 tatgtctaat aatacaagat atggtgttag tagatataca aagtgacata ttatgtctca
     1861 ttatgctttt tattattgta ataattattg tgctgcaaat tatgtactgc tgtaaaactc
     1921 aaaaactgta tattgtctct ataattaagt attatactta attataattg cctgtgataa
     1981 ttaatatcta ataaatacag ttagttatca aagtaattga tttttgctaa acatttggca
     2041 tgcagcttgc actatatcca agaaaaattt ttaggaggta gttgagtatg cctaagttaa
     2101 ttaaacgtaa aaagggacaa aaaggtttta cacttattga gctcttagta gtagccgcta
     2161 ttattggtat tcttcttgct cttgcaattc ctaaccttat taaggctaga atgtcagcaa
     2221 acgaagcaaa cgcaagaaag tctatgcaga ccctaagaga tgcagaagcc gagtattttg
     2281 aacaggatct tgatggtaat aacacaagag actatacaca gttaataggt agtttgacaa
     2341 ca
//
LOCUS       DBNGSV010000090         4823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_154964, whole genome shotgun sequence.
ACCESSION   DBNGSV010000090 DBNGSV010000000
VERSION     DBNGSV010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_154964"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /gene="trpB"
                     /locus_tag="AC159U_03225"
     CDS             complement(<1..1151)
                     /gene="trpB"
                     /locus_tag="AC159U_03225"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3336098.1"
                     /translation="MQNKYKYPDKLGHFGQFGGRYVSETLIPALLELEQAYNKIKNDK
                     SFKKEVDYYLKEYVGRPTPLYFAARMTEKLGGAKIYLKREDLAHTGAHKINNTIGQAL
                     LAAKMNKKRVIAETGAGQHGVATATVAALLDMECDVYMGLEDTKRQALNVFRMKLLGA
                     NVKEVNSGTQTLKDAMNEAMRDWVTNVRNTFYIIGSVAGPHPYPMMVRDFQSVIGNEA
                     RKQIIQKEGRLPDLLVACVGGGSNAMGLFFPFIQDKHVKKVGIEAAGEGIKTDRHSAS
                     ISKGKVGVLHGSKTYVLQDDDGLVKGAHSIAAGLDYPGVGPEHSYFNDQKLISYKSVS
                     DTEALQAFKYLSEVEGIIPALETSHAIAHVIKIAPKMPKDSVIVVCLSG"
     gene            complement(1141..1755)
                     /locus_tag="AC159U_03230"
     CDS             complement(1141..1755)
                     /locus_tag="AC159U_03230"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3336099.1"
                     /translation="MTKVKICGITNYKDASKSLDYGADALGFIFYKKSKRYIEPSEAL
                     NIIKQLPPFVTKVGVFVNSSYTNITKISKTLSINAIQLHGDETPAFCKRFLPVVIKAL
                     RIKDHQDLEKVDSFGTQAILFDTYSSADYGGTGRVFDWNILRKLKTDKKIILSGGLNP
                     QNVGMAVKTVKPYAVDVSSGVEKSAGIKDHKKIKEFIEVVKNAK"
     gene            complement(1812..2783)
                     /locus_tag="AC159U_03235"
     CDS             complement(1812..2783)
                     /locus_tag="AC159U_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3336100.1"
                     /translation="MKSLSIKITFLLVAMAVVSCHGHFAVDTHRHDKYKNVEMDYDKG
                     YDEVWSAALNSLENTNWDIKKADKEKGEIHLQPSFVYTPEFDMLSRIYVEPTNQDIEE
                     SFIKPYLRSISYYEKTTEMDPKFVRETLNLKFIPVDDNHTKVKANYKIEPYYSYKIGY
                     IGSVRSNGEFEKGLFSDMHGILYPPMIAAPPPPPMPDDLDLNLKVIFFDFNRFEIRDD
                     AKPILMENAQIIKDNPDVTFLVESFADIRGTQQYNHRLAQNRADMTRQFLIEQGVERD
                     RIISVGRGETDMYGAEKTKEVYQLNRRSRLSHTRSLDLSKYFTKSDK"
     gene            complement(2798..3166)
                     /locus_tag="AC159U_03240"
     CDS             complement(2798..3166)
                     /locus_tag="AC159U_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336101.1"
                     /translation="MMNEIKIKQNLTVLIIVQFIFCFHILADKAGAADEFSLTASQKH
                     FTSILRGLPGIMSVSWESPVSLWIRTSSKAVGSPPNIKKAQNLAKTLAERGKTAMRQP
                     LCVHIYQTKSKELAKSCVFF"
     gene            3360..3935
                     /locus_tag="AC159U_03245"
     CDS             3360..3935
                     /locus_tag="AC159U_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3336102.1"
                     /translation="MEPILAILVGIGLAAATGFRIFVPFLVISVAANSGHLTLAEGFE
                     WIGTEVAIGTFLVATILEISAYYVPVLDNVLDSVATPASFIAGTIVMASMVSDFSPYL
                     QWALAIIAGGGIAASVQSLTAITRLTSTATTGGLGNPLFSTAEAGGSTLLSIAAIFIP
                     IVAFLFVVGLLYFVYKKIILRLFRKPESGLT"
     gene            complement(3928..4206)
                     /locus_tag="AC159U_03250"
     CDS             complement(3928..4206)
                     /locus_tag="AC159U_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336103.1"
                     /translation="MYKTLLSLLFIITLAVLNTSCVPYSYQDMTEAEIKYKDCLKRAK
                     KNKEFCNNLKYDYQMKAEDYAKDAEDFWYIMGLETEEEKIIEQYREFK"
     gene            complement(4223..>4823)
                     /gene="hemG"
                     /locus_tag="AC159U_03255"
     CDS             complement(4223..>4823)
                     /gene="hemG"
                     /locus_tag="AC159U_03255"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR3336104.1"
                     /translation="DKYFADGIILATSSYISANILSSSDPALSEELSKIEFASSSIVI
                     LGYKKEEIKNNLAGFGFVVPAKENLNIIACSYSSEKFPGRAPRDTVLLRCFVGGALNK
                     KFLNNNDNEIIDIIKEELRTILHINCEPEFTHLKRYPNSMPQYNVGHSEILSNIEKLM
                     SNHPSLQLAGNSYEGVGIPDCIRWGEVAAENIIKNNSSN"
BASE COUNT         1326 a          919 c          847 g         1731 t
ORIGIN      
        1 cttcctgaaa gacatacaac gattacagag tctttaggca ttttgggtgc gattttaata
       61 acatgagcaa tggcatgaga ggtttctaaa gcaggaatta taccttctac ttctgacaga
      121 tacttaaagg cctgcaacgc ttcagtatca cttactgatt tatatgagat tagtttttga
      181 tcattgaaat aagagtgctc aggacctact ccgggataat cgagtcctgc ggcaatcgag
      241 tgcgctcctt taactaggcc atcatcgtct tgaagcacat atgttttact gccgtgaagt
      301 acacccactt tacccttact tatgcttgcc gagtgtctgt ctgtttttat tccttctcct
      361 gcagcctcaa ttcctacttt tttgacatgt ttgtcctgga taaaaggaaa gaaaagtccc
      421 attgcattag atcctcctcc cacacaagcc actagcagat caggtagtct tccttctttt
      481 tgtattattt gtttcctggc ctcatttcct attactgatt gaaaatctct taccatcata
      541 gggtaggggt gaggtcctgc tacactgcct ataatataaa aagtatttct cacattagta
      601 acccagtctc tcatggcttc gttcatggca tcttttagag tttgggtgcc agagtttact
      661 tcttttacat ttgctcccag aagcttcatc ctgaatacat tgagtgcctg tctcttggtg
      721 tcttcaagtc ccatataaac atcacattcc atatcaagca gagctgctac tgtagctgtt
      781 gctactccat gctgtcctgc tccggtttcg gcaatcactc ttttcttgtt catttttgcg
      841 gcaagaagag cctgtccaat agtattattt attttatgtg caccggtatg agcaaggtct
      901 tctcttttaa gatatatctt agctccaccc agtttttcgg tcattctggc tgcaaaataa
      961 agaggggtgg gcctgcctac atattctttt aagtaataat caacctcttt tttaaagctt
     1021 ttatcatttt ttatcttatt ataagcctgt tccagctcca gtagtgctgg aatcagagtt
     1081 tcagatacat acctgccgcc aaactgtcca aagtgcccaa gtttatcggg atacttgtat
     1141 ttattttgca tttttaacaa cctcaataaa ttcttttatt ttcttatgat ctttaatccc
     1201 tgcagatttt tcaacccctg agcttacatc aactgcatat ggttttacag tctttacagc
     1261 cataccaaca ttttgcggat ttaatccccc ggagagtata atctttttat cagttttcaa
     1321 ttttcttaaa atattccagt caaaaactct gccggttcca ccataatctg cactcgagta
     1381 tgtgtcgaaa agaatagcct gagtgccaaa agaatcaacc ttttctaggt cttgatgatc
     1441 tttaatcctt aaggctttta tgactacagg cagaaatctt ttacaaaaag caggagtttc
     1501 gtcgccatgc agctgaattg cgttaatcga taatgtcttg gatattttgg ttatattggt
     1561 atatgaagaa tttacaaata cgcccacttt ggttacgaaa gggggtaatt gttttataat
     1621 atttaaagct tcggagggtt caatatatct tttgcttttt ttataaaaga taaatcccag
     1681 ggcatctgca ccatagtcca gactctttga agcatcttta taattagtga ttccgcaaat
     1741 tttaactttg gtcatagtat gtttttacat actataacca aagatttatt tgttagatag
     1801 taggttttgt actacttgtc agattttgta aaatatttgc taaggtcgag tgatcttgta
     1861 tggcttaatc ttgatcttct gtttaactga tatacttctt ttgtcttttc agctccatac
     1921 atatcagtct ctcctcttcc taccgatata attctgtctc tttctacacc ctgttcaatc
     1981 aggaactgtc ttgtcatgtc agctctgttc tgagcaagtc tgtgattata ttgctgagta
     2041 cctcttatat ctgcaaaaga ttcaacaaga aaagttacat cagggttatc ctttatgatc
     2101 tgtgcatttt ccataagaat aggttttgca tcgtctctta tctcaaatct gttaaaatca
     2161 aagaatatga cttttaggtt aagatcaaga tcatcaggca ttggtggggg tggaggagct
     2221 gcaatcatag gtggatataa aataccgtgc atatctgaaa acaggccttt ttcaaactca
     2281 ccgtttgatc ttacgcttcc aatatatcct attttatagc tgtaataagg ttcaatctta
     2341 tagtttgcct ttactttggt atgattatca tctacaggta tgaactttaa atttagtgtt
     2401 tcccttacaa acttaggatc catctcagta gtcttttcat aatatgaaat agatcttaaa
     2461 taaggtttta tgaatgattc ctcaatgtcc tggtttgtag gctcaacata tatcctgctc
     2521 agcatatcaa attccggagt gtatacgaat gagggctgta gatgtatttc acctttttcc
     2581 ttgtcagcct ttttaatgtc ccaattagta ttttccagtg aatttaatgc cgcagaccag
     2641 acttcatcgt agcctttgtc gtagtccatt tcaacatttt tatatttgtc atgccggtga
     2701 gtatcaacag caaagtgtcc atgacacgaa acgactgcca tggcaaccag taaaaaggta
     2761 attttaatac ttaatgattt catcgtcctc cctcctgtta gaaaaataca caagattttg
     2821 ctaattcttt actttttgtt tgatatatat gtacacatag tggctgtctc attgccgttt
     2881 tacctctctc ggcaagagtt ttagcaagat tctgggcttt tttaatgttt ggtggagaac
     2941 ctacagcttt tgaagaggtt ctgatccaaa gtgaaactgg tgattcccag cttacgctca
     3001 taattccggg aagccctctg agtatagaag taaagtgctt ttgagaagct gtaagagaaa
     3061 actcatcagc ggcacctgcc ttatcagcta atatgtgaaa acaaaatatg aattgtacta
     3121 taattagtac agtcagattc tgcttaatct ttatttcatt catcatttgt tgcttcaaat
     3181 gctattaaat tttactaaat tataaaactt aactttaact ataacacata actactttaa
     3241 tttaaataac ttttttctaa ttatataata aacctaaata gtaatcaagt gttttttttg
     3301 aaacaaattt gaggcttatt ggttaatata tttaaaaaca tttaaaaagg agagtctaaa
     3361 tggaacctat tcttgcaatt ttagtaggta ttggccttgc cgcagccacg ggatttagga
     3421 ttttcgttcc atttttagtt ataagtgttg cggcaaattc agggcattta acccttgccg
     3481 aaggatttga atggattgga acagaggttg cgatcgggac attcctggta gcaacaattt
     3541 tggaaataag cgcatattat gtgccggttt tggataatgt gcttgattca gtggctacac
     3601 cagcgtcatt tattgcaggt actattgtaa tggcatccat ggtatctgat tttagccctt
     3661 atcttcagtg ggcacttgca ataatagcag gaggtggaat agcagcatcg gtacagagct
     3721 tgaccgcgat cacaaggcta acatcaacag ctactacagg tgggcttgga aaccctcttt
     3781 tttctactgc tgaagctggt ggttcaacat tactatcgat tgctgcaata tttatcccga
     3841 tagtagcatt cctgtttgtt gttgggcttc tatattttgt ttataaaaag ataatcctca
     3901 ggctttttag aaagcctgag agtgggctta cttaaactct ctgtactgtt caataatttt
     3961 ttcttcttca gtttctaaac ccataatata ccaaaaatct tctgcgtctt tggcataatc
     4021 ctcggctttc atttggtagt cgtattttag gttattacaa aattctttat tctttttagc
     4081 tctttttaag cagtctttat atttaatttc agcttctgtc atatcttgat agctgtatgg
     4141 aacgcaactt gtgtttaaaa ctgctagagt aattataaaa agtagtgata gtaatgtctt
     4201 atacattgct ataagtatag ctttaatttg aagaattgtt cttaatgata ttttcagcag
     4261 ctacctcacc ccatcttata caatcaggta ttcctacccc ttcataggag ttgcctgcta
     4321 gctgtagact gggatgattg gacatgagtt tttctatatt agataagatt tcagaatgtc
     4381 caacattgta ttggggcata gagttagggt agcgttttag gtgtgtgaac tctggttcac
     4441 aattaatatg caaaatagtt ctaagctctt cttttataat atcgataatc tcattgtcat
     4501 tattgttaag aaatttcttg tttagtgcgc cacctacaaa acacctcagc agcactgtat
     4561 cccttggcgc tcttccaggg aatttttcac tgctatatga gcaggcgata atattcaagt
     4621 tttcttttgc cggtactaca aatccaaaac cggccaaatt attttttatt tcttcttttt
     4681 tatagccaag gattactatt gaagatgatg cgaattctat ctttgataac tcttcagaca
     4741 gagcggggtc tgatgaactg aggatatttg ctgatatata agatgatgtg gctaatataa
     4801 ttccatcggc aaaatattta tca
//
LOCUS       DBNGSV010000091         3299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1194086, whole genome shotgun sequence.
ACCESSION   DBNGSV010000091 DBNGSV010000000
VERSION     DBNGSV010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1194086"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1701
                     /locus_tag="AC159U_03260"
     CDS             172..1701
                     /locus_tag="AC159U_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3336105.1"
                     /translation="MISKVFSSAVFGIDAYLVEVEVDISSGFPGFTTVGLPEGAVKES
                     KERVVAAIKNCGFKFPQKRITVNLAPADRKKEGSALDAPISVGILSASGLIDLIKLKD
                     YVILGELSLDGKIKGIKGTLPSALCVRDKGLKGIILPRENAAEASVVNGIEVIPVDRL
                     SQIIEFLAGNIDIEPATVDLSEVFNQNNSYPVDFQEVRGQEHVKRSIEVAAAGGHNIL
                     MIGSPGTGKTMLARRLPTILPNLSFEEALETTKIYSVSGLLPSNSSLIATRPFRSPHH
                     TISDAGLIGGGAIPKPGEVSLAHNGVLFLDELPEFKKNVLEVMRQPLEDGKVTIARAS
                     TSITYPSTLMLVTAMNPCPCGFYSDPNKECTCSPMHIQRYRSKVSGPLLDRIDIHVEV
                     PPVKYKELSSEAGGEKSSDIRKRVNTARDIQKNRFAKVGIHSNAQMTPKLVRQYSKPD
                     EAGLKLLETAIDKLGLSARAYDRILKVARTIADLEKSYKVLSTHISEAIQYRSLDREV
                     I"
     gene            1762..2370
                     /locus_tag="AC159U_03265"
     CDS             1762..2370
                     /locus_tag="AC159U_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336106.1"
                     /translation="MKLERKNHWEILLNSWNNDTPACMHELALEYTKRFPDDFCGWIV
                     LADTLTHFALYKRARAALSTALSLAPTEKKTDVYHQIGHHYSEKGDHKRAELWYRKAL
                     EENRCVEHYIFLGACLAKQGRLEKAKKYHLQAIKTKSKEVDEAYFNLGLIYRAERNFK
                     KASEYFGKAIELDPEYKLAKIAKEDINKIEDHLKKIKQDREN"
     gene            2505..2993
                     /locus_tag="AC159U_03270"
     CDS             2505..2993
                     /locus_tag="AC159U_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3336107.1"
                     /translation="MCEDPIDESSLGLQWFSKGLLGKHLAIDFNAPAGTEVRAIADGH
                     IEHNYTKMRYYGGCDGKPGPVVITRHSGGSHGSYAVQYGHVSSDLKENDLILAGDVIG
                     KVINYKPCCDFPEACPHVHFAIWDSPTEHPTSGMGYGNPRSFVNPDWFFKNNLCITGT
                     AE"
     gene            3064..>3299
                     /locus_tag="AC159U_03275"
     CDS             3064..>3299
                     /locus_tag="AC159U_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR3336108.1"
                     /translation="MIRILVILLMAMSTIGAEISEAENQITVKSMPPVVVKTFPQAGD
                     TGVDPSIKEIKVTFSKDMMTEQMWSWVMASKDSFP"
BASE COUNT         1070 a          622 c          721 g          886 t
ORIGIN      
        1 tccaatacaa acatttgtat tagcttgctt atcttactat ttgatagttt tgtattctta
       61 agtaacttct tttgcatagt ttcagcttag tatactttgc tgtcctatgc gagctcctaa
      121 attaataact tcctaaattc atcataacat actaatataa tatgtttaac aatgatctct
      181 aaagtattta gtagcgcagt tttcggaata gatgcatatt tggtggaagt agaggttgat
      241 atttcctctg gttttccggg gtttacaaca gtgggtcttc ccgagggtgc agtaaaggaa
      301 agcaaggaac gtgttgtagc cgccataaaa aattgcggct ttaagtttcc gcagaaaaga
      361 atcacggtaa acctggcacc tgcagacagg aagaaggaag ggtcagcact tgatgcgccc
      421 atatctgtag gaatattgtc tgcatcgggt cttatcgatc tcattaaact aaaagactat
      481 gtaatcctgg gcgagctctc tcttgatgga aaaataaagg gcattaaagg tactctgcct
      541 tctgcgcttt gtgtaaggga taaaggactc aagggaataa tacttccaag agaaaatgcg
      601 gccgaggctt cagtggtaaa tggtatagag gtcatccctg tagacagatt atcacagata
      661 atcgagtttt tagccggcaa tattgatata gaacccgcaa cagttgatct aagcgaggta
      721 tttaatcaga acaacagcta cccggtagac tttcaggaag taagggggca ggagcatgta
      781 aaacgctcta tagaagtggc tgctgccgga gggcataaca tccttatgat cggctcccct
      841 ggcacaggaa agaccatgct tgcaagaagg cttccgacaa tacttccgaa tttaagcttt
      901 gaagaggcat tagaaactac aaagatttac tcggtttcag gtttactccc atctaattca
      961 tccttaattg ccacaagacc ttttcgttct ccgcaccata caattagcga tgcgggactc
     1021 attggcgggg gagctattcc taaacccgga gaagtgagcc tagcacataa cggcgtgctt
     1081 tttttggatg agcttccgga gtttaaaaaa aatgtgcttg aagtcatgcg ccagcctctt
     1141 gaagacggaa aagtaacgat agcaagagct tcaacgtcta ttacataccc atctactctt
     1201 atgcttgtaa ctgcgatgaa cccctgccca tgtggatttt atagcgaccc aaacaaagaa
     1261 tgcacctgct caccgatgca tatacagaga tacagatcaa aagtatccgg cccgcttctg
     1321 gacaggattg atattcatgt agaagttccc ccagtaaaat acaaagagct ttcaagtgaa
     1381 gccggcggag agaaatctag tgatataaga aaaagggtaa acacggcaag agatatccag
     1441 aaaaacagat ttgccaaagt tggcattcat tcaaacgccc aaatgactcc aaagcttgta
     1501 agacagtatt ccaagcctga cgaagccgga ctcaagctac ttgaaaccgc aattgataaa
     1561 cttggcctca gtgcacgggc ttatgacaga atcctaaaag tagcaagaac tatcgccgac
     1621 ctcgaaaaat cttacaaggt actttcaacc catatctccg aagctattca gtataggtcg
     1681 cttgataggg aagttatttg agaaatttag tgtcattatt attgtgaata accataaaaa
     1741 taaatgttag attaaaataa tatgaaactt gagcgaaaaa accattggga aatccttttg
     1801 aactcatgga ataacgatac gcctgcatgt atgcatgaat tagcattaga atatacaaag
     1861 cgttttcctg atgatttttg tggatggata gtgttagcag acactttaac ccattttgct
     1921 ttgtataaaa gggcaagggc tgctttaagt accgcacttt cgttagcacc tactgagaaa
     1981 aaaaccgatg tctaccacca aattggtcat cattattctg aaaagggaga tcataaacga
     2041 gcggagcttt ggtatcgtaa agcgctagaa gaaaatagat gtgttgaaca ttatattttt
     2101 ctcggtgcat gccttgccaa acaaggaagg ctcgaaaaag caaaaaagta tcatttacaa
     2161 gccataaaaa caaaatcaaa agaagtagat gaggcttact tcaacctggg gttgatctat
     2221 agagccgaac gaaattttaa aaaagctagc gagtattttg gtaaggcaat tgagttagat
     2281 cccgaatata agttggccaa gattgccaaa gaggacataa acaaaatcga agatcatcta
     2341 aaaaagatca aacaagacag agaaaactga ccgttacccg ctaaaacata gatataacaa
     2401 atcaaatgca ctcggatttc aaaaagcgtt tattatttta tctatttcta tggataccat
     2461 tcattttatt tatttccagt tgtgcagaaa ccaaaattga aagcttgtgt gaagacccga
     2521 ttgatgagag tagtctgggt ctgcagtggt tttccaaagg gctgttaggc aagcacctgg
     2581 ccattgattt taatgcaccc gcaggaaccg aggttagggc aattgccgac ggacacatag
     2641 agcataacta tactaaaatg agatattatg gcggctgtga tggaaaaccg ggtccggttg
     2701 taataaccag gcattcgggg ggaagccatg gctcctatgc cgttcagtat ggacatgtca
     2761 gctctgatct aaaagaaaac gatttaatac tagcaggcga tgtgattgga aaggtaataa
     2821 actataaacc atgttgtgat ttcccagagg catgtcccca tgtgcatttt gcaatttggg
     2881 attcacccac cgaacatcca acgtccggga tgggttatgg aaacccacgc agctttgtaa
     2941 atcccgattg gttttttaag aacaatctat gcattactgg tactgctgag tgaagagggg
     3001 aagttttatg agaaatttga tgtcataatt attgagaaca agcataaatt aaaggaggtt
     3061 catatgatac ggattttagt aatattactt atggcaatgt cgacaatcgg tgctgaaata
     3121 tctgaggcag aaaatcaaat tacagttaaa tcaatgccgc cggttgtggt aaaaacattt
     3181 cctcaggcag gggacaccgg ggttgatcct tctataaaag agataaaggt cacattcagt
     3241 aaggatatga tgactgagca gatgtggtca tgggtaatgg catctaagga ttcgtttcc
//
LOCUS       DBNGSV010000092         1555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1678948, whole genome shotgun sequence.
ACCESSION   DBNGSV010000092 DBNGSV010000000
VERSION     DBNGSV010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1678948"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="AC159U_03280"
     CDS             <1..344
                     /locus_tag="AC159U_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3336109.1"
                     /translation="NGIEIGGGSIRIHRKDIQQKIFRAIGMDEQEANSRFKFLLEALE
                     FGAPPHGGIALGLDRLIMLLSGATSIRDVIAFPKTQKAFCPLTEAPSEVDLNQLLELG
                     INIKKKQNDGS"
     gene            445..1398
                     /locus_tag="AC159U_03285"
     CDS             445..1398
                     /locus_tag="AC159U_03285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3336110.1"
                     /translation="MSAKPTVSIVIPLYNEEESFPILVQRLNKLKKELPFKIEVVLID
                     DGSTDRTSELMEELSLSDSTYKSVFLSRNHGHQLALTAGLANVSATEAVMIIDGDLQD
                     PPELLEQMYEKYKQGYDVVYAIRTKRKEDFFKRVSYWLFYRLLNLISNFNIPLDSGDF
                     SLISMRVVNIMNKMPEESRYLRGMRSWIGFKQTGIVYERDERSYGETKYTLRKLIGLA
                     YSGVFNFSDFPIKFITYLGLISILFSFLYLAFTIIHKLIYQNVPQGFTTTLFIIILLS
                     GVQLVSLGVIGEYVTRIFFQVKNRPLYTISQIIENKKPKDG"
BASE COUNT          541 a          267 c          301 g          446 t
ORIGIN      
        1 taaacggaat agaaataggc ggaggaagca taaggattca cagaaaagat atacaacaaa
       61 agattttcag agcaatcggc atggatgagc aagaagcaaa cagcaggttt aagtttctgc
      121 tcgaagcctt ggagtttgga gctcctcctc atggcggtat agcgcttggc ctggacaggc
      181 tcataatgct gctttcgggg gccacctcta taagggatgt aattgcgttt ccaaaaacac
      241 agaaagcctt ctgcccgctt actgaagcac catctgaagt ggacttaaac caactactgg
      301 agcttgggat taatatcaag aagaagcaaa acgatggcag ctaaattctt ttatatttat
      361 gatctgaaaa tagaataatt tatatagtat tagtacttat acatatatta atttagatta
      421 gtttaggtta ttatacttat tcaaatgagt gccaaaccca cagtttcaat agtaattcca
      481 ctgtacaatg aagaggaatc atttcccata ttagtgcaga gattaaataa acttaaaaaa
      541 gaactaccat tcaaaattga agtggtacta atagatgatg gaagcacgga tcgcacatct
      601 gaacttatgg aagaattgtc actcagtgat agcacttaca aatctgtttt cctttccaga
      661 aatcatggac accagctagc actaaccgca gggcttgcaa atgtatcggc tactgaagcc
      721 gttatgataa tagacggaga cctgcaggac cctccggagc tgctggaaca gatgtatgaa
      781 aaatacaaac agggctatga cgttgtatat gcaataagaa caaaaagaaa agaggacttt
      841 ttcaaaagag tttcatactg gcttttttac agactgttaa atctgatttc taattttaat
      901 atacctctgg atagtggaga tttctctctt ataagcatga gagttgtaaa tattatgaat
      961 aagatgccag aagagagccg atacctgaga ggcatgagaa gctggattgg atttaaacaa
     1021 accggtattg tatatgaaag ggatgagaga agttatggcg agacaaaata tactctcaga
     1081 aagctaatcg gacttgcata ttccggagtt tttaatttta gtgattttcc tattaaattc
     1141 attacatatt tagggttaat atcaatactt ttttcattct tatatttagc tttcactatt
     1201 atacacaaac taatttatca aaatgtcccc caaggcttca caacaacttt gtttataatt
     1261 atactgctaa gcggggtaca gctggtgtcc ctgggagtaa taggtgaata tgtaacaaga
     1321 atattctttc aggttaaaaa caggcctcta tatacaataa gtcaaataat cgaaaacaaa
     1381 aaacctaagg atggatagag cttattataa tgagtaccac ttcctggagc ctaatcattg
     1441 gtggtttgtt gtacgttcca ggattattaa tgacgcaatc aaaagactca taaattccaa
     1501 taaaaacctc tcgatactaa acattggcat tgccaaaggt gccacaaatc aattt
//
LOCUS       DBNGSV010000093         7853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1609521, whole genome shotgun sequence.
ACCESSION   DBNGSV010000093 DBNGSV010000000
VERSION     DBNGSV010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1609521"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC159U_03290"
     CDS             complement(<1..857)
                     /locus_tag="AC159U_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR3336111.1"
                     /translation="MEDTILYNISLFIVGLILLYFGGEGLVKGASRLARALGINSIVI
                     GLTVVAFGTSAPELVVSIIASVKGSNDLIMGNILGSNISNIGLILALTALIYSIRIQL
                     TLIKVELPFMILLSTLLFILSKDLEIGRIEGAALFALLISIITYSSYVAFKEPKYIER
                     EFDEFIEVDKNYYKNFAYILIGLIGLSIGAKLIVESSITMAQKFGVSETIIGITAVAI
                     GTSLPELTTSIIAALKKESGIIIGNIIGSNIFNIGIIGIVSILKPIQVESRLLDFEFL
                     VMIFLSVLML"
     gene            1101..2825
                     /locus_tag="AC159U_03295"
     CDS             1101..2825
                     /locus_tag="AC159U_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3336112.1"
                     /translation="MRLLFKPRGSKYVGEGTLPHGTFDDTTQWLPITRYLEMGAEQYP
                     DKTLFRVADNDGNLTESYTYKESNEWANRVSNGLISDYGVKKGSKVGMYMINCSEYVV
                     SIIAIHKAGAIQVPINKDEKGERLAYVINYSDMISLIIDQGSIPLIEEVAKDLTNLQS
                     IIVTGEGDIPSEIGGIKVTSFKNFDSGSNANPGMNVNTTDQERCMFTSGTTGMPKGVV
                     REHGGVIMTVRSYLQQQGVRSGDVLMSVLSLGHANAQVMCLFSAIGSGATAVFYPRFS
                     ASSFWKWASECGATCANMLGAVAEYLWAAPPSEWDKKHNIRIMLGSPAPRNLAEFQER
                     FGVRVIDGYGSTEMGMVLWKDAEDHRPGSSGYPMEGYYTELRNPLVQDEAIRAFWDPF
                     ANPTPPEEAKGLLYIRPMIPNTTLNEYFKDDRRTREAFDDDGFFNSDDIFARGMDGRY
                     YFVGRFSRIRVSGENVDPVAVADEALKYDSIQDAIAVGIRLPNVSDDEIKLNVTLKSG
                     ANFDPIEFCKWMAERVMVAMVPRFIEVFEDGFPMTATQKVKVAELKEITDKTWDRSTT
                     GLKFSARK"
     gene            2951..3703
                     /locus_tag="AC159U_03300"
     CDS             2951..3703
                     /locus_tag="AC159U_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3336113.1"
                     /translation="MSKESEYIIEFVDVHKSFNSNHVHRGINLGVKKGENICILGGSG
                     SGKSVILKELCGLIEIDKGKILVEGEDITSLDEGQVLRVRKKIGMLVQGSALFDSLDV
                     YENIAFPIRENFKYSEDQIGSIVRENLDIIGLEGIEDKYPSDLSGGMKKRVALARALA
                     MKPKILLYDEPTTGLDPINIKRINQLIRDMQSRFSMTAIIITHDIVSSAFEVSDRVAF
                     LYDGKIIFYGTVEQAKNTEIKEFRDFIEGRYN"
     gene            3732..4481
                     /locus_tag="AC159U_03305"
     CDS             3732..4481
                     /locus_tag="AC159U_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336114.1"
                     /translation="MNILIVHDSKSSILLIKTFIMSELSDALVSESTDVESGEQLLKN
                     SKFDVIICGKQVIENGSAGFISKVKSMNRNCPIILVTSDSSNEKLNDIKASGINYVLS
                     TPFNSVQLRSIILEACDLRTRRAHQRINIPGTKVIAHLDHDDLDGEVINISESGILCD
                     FIGKKINSELINSTHVSVVFPPIFNNASIKNLSCKLLRLNVMAWDDDLFPECLPNHVR
                     VVWQVVNMSFENKKILTDILSIAENNLSKLH"
     gene            complement(4555..5631)
                     /locus_tag="AC159U_03310"
     CDS             complement(4555..5631)
                     /locus_tag="AC159U_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3336115.1"
                     /translation="MAELDKFFNYMAENDISDFHLSSGMRPLFRHHGELKPASDQILT
                     HDIVTKLCNEILDEEQKKTFKQNNDLDFAHEVTGVARFRANYYMQQRGISSAFRLIPK
                     EINSIENLGLPEKIKDFASLSRGLVLVTGPTGSGKSTTLAALINHINKTRSDHIITIE
                     DPIEFVHQSDKCLVQHRQVGVHTESFSTALRASLREDPDIIMLGEMRDLETIELAITA
                     AETGHLVFATLHTSSAPKTIDRIIDAFPAGQQSQIRTMLSESLKGVVCQQLLKRSDIA
                     GRSAALEILVVNTAVSNLIREGKIFQIPSIIQTGKQQGMQLLDNAIMQLHKDGKISAE
                     EAFYKSNDKQMFEHLIENKQHTLN"
     gene            complement(6031..7089)
                     /locus_tag="AC159U_03315"
     CDS             complement(6031..7089)
                     /locus_tag="AC159U_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3336116.1"
                     /translation="MAEIDKFFNYMVENNISDLHISSGSRPLFRQFGELKPASDQILT
                     HDVVKKLLYEILNEEQTQVFNERNDLDFAHELTGLARFRVNYFMQQRGMSGAFRLIPT
                     TVESLDTLGFPEILKSFSALSRGLVLVTGPTGCGKSTTLAAIVDHINKTRTDHIVTIE
                     DPIEFVHTSDKCLIHHRQVGIHTKSFSGALRASLREDPDIIMLGEMRDLETIELAITA
                     AETGHLVFATLHTSSAPKTIDRVIDAFPAGQQAQIRTMLSESLKGIVCQQLLKRTDKP
                     GRVAALEILVVNTAISNLIREGKIFQIPSVIQTGKQLGMQMLDAAIMKYYKDGVISAE
                     EAFYKSNDKQQFEKFIKE"
     gene            complement(7240..>7853)
                     /locus_tag="AC159U_03320"
     CDS             complement(7240..>7853)
                     /locus_tag="AC159U_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3336117.1"
                     /translation="VNVAIASIIFTFLYLSGTTIPIINIDISADSFLVKLMALNIILV
                     LFNMLPAFPMDGGRVLRALLALKMEYVKSTTIASKIGKAMAILFGILGVLSYDSNPFL
                     IVIAVFVWFGANHEENIIKIKHYRESYSNKEPEITTGLINLCPNDPLARAVNLTISGV
                     KQDFAVMEDGKIVGVLRINDLMDTLSKGNINYLVKDIMRTDLK"
BASE COUNT         2490 a         1302 c         1505 g         2556 t
ORIGIN      
        1 agcattagta cagatagaaa gatcattact aaaaactcaa aatcaagcag cctggattcc
       61 acctgaattg gttttaatat agaaacaatt cctataatac caatattgaa aatattactt
      121 ccaattatat tcccaatgat tattcctgac tcttttttta atgcagcaat tatagaagta
      181 gtaagctcag gaagggaagt gccaatggct actgcggtaa ttccaattat tgtttcactt
      241 actccaaact tttgagccat ggttattgaa gattcaacaa taagttttgc cccgatgcta
      301 aggccaataa gtcctatcag tatataagcg aaatttttat aatagttttt atcaacttca
      361 atgaattcat caaactctct ttcaatatat ttaggctctt taaacgcaac ataactacta
      421 taagtaataa ttgaaattaa aagagcaaaa agagcagcac cttcaatacg tccaatttct
      481 aagtccttgg ataatataaa caacagggtt gacagcagta tcatgaaggg aagctcaact
      541 tttattaatg taagctgtat tctaattgaa taaataagtg ctgttagtgc cagtattaat
      601 ccaatattgg aaatattact tccaaggata ttccccatta ttaaatcatt cgaaccctta
      661 acagaagcta ttatcgatac aaccagttcc ggggctgatg ttccaaatgc taccaccgtt
      721 agtccaatga caatagagtt tattcccaat gcccgagcta atctggaggc tccctttaca
      781 agcccctcac cgccaaaata tagtaaaata aggccgacta taaataacga tatattgtat
      841 aggatagtat cttccatatg tccattaaga attaaatgag tctttattaa aggttttttc
      901 tttgattctt tttatatatt tatttgaaat tagtcggcta atgcattaaa attacttgtt
      961 ttagtataat attaaaggaa aaataggtaa aaagatatcg aacatgttat tttgtcatga
     1021 gtgaaaaact gcgtgacaaa ataatcttta tatgtttata ctaaaaaata attttttcaa
     1081 atatatggag gaggggttaa atgcgattac ttttcaaacc tagaggttct aaatatgtag
     1141 gagaaggcac gctcccgcac ggcacatttg atgatactac tcagtggctg ccgatcacaa
     1201 gatatctgga aatgggtgct gagcaatatc cggataaaac tctatttcgt gtggctgata
     1261 atgatgggaa tttaactgaa agctacacat acaaagagtc taatgaatgg gcaaacagag
     1321 tctctaacgg acttataagc gactatggag taaaaaaggg cagtaaggtt ggaatgtata
     1381 tgattaactg ttctgaatat gttgtttcaa ttattgctat tcacaaagct ggtgcaatac
     1441 aggtacctat caataaagat gaaaagggtg agagattagc atatgtaatc aattactctg
     1501 atatgatttc cttgataatt gaccagggaa gtattccact tatagaagaa gtagcaaaag
     1561 acttaactaa tctacaaagt attattgtaa caggtgaagg tgatatacct tcagaaatag
     1621 gtggcattaa ggtaacatca tttaagaatt ttgatagtgg ctcaaacgca aatcctggta
     1681 tgaatgtaaa cactacggat caagaacgtt gtatgtttac ttcaggtaca acgggtatgc
     1741 ctaagggtgt agtaagagaa catggcggag ttataatgac tgtaagaagc tacctgcagc
     1801 agcagggagt tagaagtggt gatgtgttaa tgagtgtact ttcactggga catgccaatg
     1861 ctcaggttat gtgtcttttt agcgcaattg gctcaggtgc tactgctgta ttttatccac
     1921 gcttttctgc ttcaagtttt tggaagtggg catcagaatg cggagctaca tgtgcaaaca
     1981 tgttaggcgc agtagcagaa tacctatggg cagcaccgcc tagtgaatgg gataaaaaac
     2041 acaatatcag aattatgctt ggatctccgg caccaaggaa cttagccgag tttcaggaaa
     2101 gattcggcgt aagggttata gatggttatg gctcaacaga aatgggtatg gttctttgga
     2161 aagatgcaga agatcacaga ccaggctcat caggatatcc aatggaaggt tactacaccg
     2221 aactcagaaa ccctctagtt caggatgaag caataagagc tttctgggat ccgtttgcaa
     2281 acccaacacc acctgaagaa gcaaaagggt tactctatat cagaccaatg attccaaaca
     2341 cgactttaaa tgaatatttt aaagatgaca gaagaacaag agaagcattt gatgatgatg
     2401 gattttttaa ttcagatgac atttttgcaa gaggtatgga tggtagatac tattttgttg
     2461 gcagatttag ccgtataaga gtatctggag aaaatgttga tccggtagct gtagctgatg
     2521 aagcgttgaa atatgattct attcaagacg cgattgctgt tggtataagg cttccaaatg
     2581 tctctgatga tgaaatcaag ctaaatgtta ctctaaaatc aggagccaat tttgatccaa
     2641 tagagttttg taaatggatg gccgagagag taatggttgc tatggttcca agatttattg
     2701 aagtatttga agatggtttt ccaatgacag ctactcagaa ggttaaagtg gctgaattaa
     2761 aagaaatcac agacaaaacc tgggatagaa gtacaacagg gctcaaattt agtgctcgaa
     2821 aataaacgat aaagatattt aatactataa aaggaagaag agagtgatat actcttttct
     2881 tccttttttt ttgctgttat gaaggtgata caacaaatat tgttttggta taataattgc
     2941 actatatctt atgagtaaag agtctgagta cattatagag tttgttgatg tacataaatc
     3001 gtttaactca aaccatgttc acaggggtat aaaccttgga gtgaaaaaag gagagaatat
     3061 ttgtattcta ggtggaagcg gctctggcaa aagtgtaata ttaaaagagt tatgcgggct
     3121 tatagaaata gataagggta aaattctggt tgaaggagaa gatattacat ccctggatga
     3181 agggcaggtc ttaagagtaa gaaaaaaaat tgggatgtta gttcagggat cggcgctttt
     3241 tgattccctt gatgtttatg aaaatatagc atttccgata agagaaaatt ttaaatactc
     3301 tgaagatcaa attgggtcca ttgtgagaga aaacttagat atcattggtc ttgagggaat
     3361 agaggataag tatccttctg accttagcgg aggtatgaaa aaaagggttg ctcttgcaag
     3421 agcccttgcc atgaagccaa aaattttgct ttatgatgaa cctactacag ggttggatcc
     3481 gattaatatt aagcgaatca atcaattgat aagagatatg cagtctaggt ttagtatgac
     3541 agctataatt attacgcacg atattgtatc cagcgcattc gaagtatccg atagggtagc
     3601 atttttatat gatggtaaaa ttatatttta cggaacagtg gaacaggcta aaaatacaga
     3661 aataaaagag ttcagggatt ttattgaagg ccggtataac taattactct ttaatttaaa
     3721 aggaatatta tatgaatata ttaattgttc atgattcaaa atcatcaata cttctaataa
     3781 aaacttttat catgtctgag ctcagtgatg ctttagtgtc cgaatccaca gatgtagaat
     3841 caggagaaca attattaaaa aattcaaaat ttgatgtgat tatctgtggt aaacaggtaa
     3901 tagaaaatgg ctcagccgga tttatatcta aagtaaaatc aatgaacaga aactgcccta
     3961 taattcttgt tacctctgat tcttctaatg aaaaattaaa tgatataaag gcctccggaa
     4021 ttaattatgt gcttagcacg ccgtttaatt cggtacagct tcgttctatt attctcgaag
     4081 cttgtgatct tagaaccaga agggctcatc agagaataaa tattcccgga actaaagtta
     4141 ttgcacatct tgatcatgat gaccttgatg gtgaagtaat aaatattagt gagtctggta
     4201 ttttgtgtga ttttataggc aagaaaatta acagtgagct tataaacagt acacatgtat
     4261 ctgtggtttt cccccccatt tttaataatg ccagtattaa aaatctttcc tgcaagcttt
     4321 tgaggctaaa tgttatggca tgggatgacg atcttttccc tgagtgtttg ccaaatcatg
     4381 taagggttgt atggcaggtt gttaatatgt cgtttgaaaa caagaaaatt cttacagaca
     4441 tactttcgat tgcagaaaat aacctaagca agctccatta agcagtattc agaaagtatt
     4501 cttcaataac actaaaccct ctatttacat aaactacata gcttataata tcacttaatt
     4561 taaggtgtgc tgcttgtttt caatcaggtg ttcaaacatc tgtttatcat tcgatttata
     4621 gaaagcttcc tcagcagaaa ttttgccatc tttatgaagc tgcattattg cattgtctaa
     4681 aagctgcatc ccctgctgct ttccagtttg aatgatagaa ggaatctgga aaattttccc
     4741 ctctcttatt aaatttgata cagcagtatt taccaccaat atttccaaag ctgctgatct
     4801 tccggcaata tcggatctct tgagaagctg ctggcaaact actcccttta aagactctga
     4861 gagcatggtt ctgatttggg actgttgccc agcgggaaaa gcatctatga ttctatctat
     4921 ggtttttggt gctgaacttg tgtgtaaagt agcaaaaaca aggtggcctg tttcagctgc
     4981 tgtaatagca agctcaatgg tttccaggtc tctcatttcc ccaagcataa taatatccgg
     5041 gtcttctctg agagatgccc ttagtgctgt agaaaatgat tcggtgtgca ctcccacttg
     5101 tctgtgctga acaagacatt tatcgctttg atgtacaaat tctatggggt cttcaattgt
     5161 tataatatgg tctgaacgtg ttttgttaat atgattgata agtgctgcca gtgtagtaga
     5221 tttaccgctt ccggtagggc ctgtaaccaa aactagtcct ctgcttagag atgcgaagtc
     5281 ttttattttt tcaggcagac ccaaattctc gattgagttt atttcttttg ggattagtct
     5341 gaatgcgctt gatattcccc gttgctgcat atagtagttt gccctgaatc tggctacacc
     5401 tgttacttca tgagcaaagt cgaggtcgtt gttttgctta aatgttttct tttgttcttc
     5461 atctaaaatt tcattacaca atttggtaac aatatcgtga gtgagaattt gatcacttgc
     5521 tggtttgagt tcaccatgat gcctgaaaag gggcctcatc ccggatgata aatgaaagtc
     5581 acttatgtca ttttctgcca tatagttaaa aaatttgtcc agttctgcca ttatatgtac
     5641 cgtcctatat ataaaaatct tatgtttagt tatattctaa ttatattcat tgcaaatggc
     5701 gtaccaacat atgtattttg gataactgct gggatttgtt gaaatcagtt taaaaacagg
     5761 attataactg actaatatat tcaactattg ttaatagttt gacagaaatt gtcggagata
     5821 atataaagat atgtaaatca gaagcttttt tacgcaataa tctagaaaaa gtaatctcta
     5881 tttctagcat tcagagggat ttatctaaca gttattaagg aattaggtac ccaaactgga
     5941 atttgtttcc ccaatatgtt tacaggtttg attaataatc ggatacaaaa gagtattgag
     6001 ttttatgcca tttatatctg agatgcagtt ttattctttt ataaattttt caaattgttg
     6061 tttgtcgttg gatttataaa atgcttcctc cgccgaaatc actccatctt tataatattt
     6121 cattatcgca gcgtcgagca tttgcatacc aagctgtttt cctgtttgta tcactgaagg
     6181 aatctggaat atctttcctt ctcttataag gtttgatatg gctgtattaa ctaccagaat
     6241 ctcaagtgcg gcaactcttc cgggtttgtc tgttcgtttg agaagctgct ggcaaactat
     6301 accctttagt gattcggata acatggttct gatttgtgcc tgttgtcctg cgggaaatgc
     6361 atcaattacc ctgtctatgg tttttggtgc agagcttgta tgaagtgtag caaaaacaag
     6421 gtggccggtt tctgcagctg tgattgctag ctctatggtt tccaagtccc tcatctcacc
     6481 cagcataata atatcaggat cttcacgcag gcttgctctt agagcaccgg aaaaggattt
     6541 agtatgtatt cctacctgtc tgtggtgtat cagacactta tcgctagtat gtacaaattc
     6601 aatcgggtct tctatggtta ctatatggtc agttcttgtc ttatttatat ggtctacgat
     6661 ggctgcaaga gtggtagatt ttccgcaccc cgtgggacct gtaaccaaaa ccagtcctct
     6721 tgaaagtgct gaaaaagatt taagtatttc cggaaaccct aatgtatcca gtgattctac
     6781 tgttgtgggg atgagtctaa atgctcctga cattcccctt tgctgcataa aatagtttac
     6841 cctgaacctt gcaagccctg taagctcatg tgcaaaatcg aggtcgtttc gttcattgaa
     6901 tacttgtgtt tgttcttcat ttaatatttc atagagcagt tttttaacta catcgtgtgt
     6961 taaaatctgg tcgcttgcag gttttagctc gccaaactgt ctgaatagtg gtctcgagcc
     7021 tgatgatata tggaggtcac taatattatt ttctaccata taattaaaaa acttgtctat
     7081 ttcagccata ttggaacacc tgcctgtata aattcattaa ttcaataata tacaaattta
     7141 ttcgaaaaat aaattattat ttgattatat ctcaataacc ttgatttggg actatggaat
     7201 tagtttatat gtaaaatata taaaactaaa gtttattatt tattttagat cagtacgcat
     7261 tatatctttt acaagataat tgatgttgcc ctttgacagt gtgtccatta aatcatttat
     7321 tctcaatacc cctactattt ttccatcttc cattactgca aaatcctgct ttactccaga
     7381 aatggtaaga tttacagctc ttgcaagcgg gtcatttgga caaagattta ttaacccggt
     7441 agttatttct ggctctttgt ttgaatatga ctctctgtaa tgttttatct ttataatatt
     7501 ttcttcatgg ttagccccaa accatacaaa tacagcgatt actattaaaa atggattact
     7561 gtcataggat aacacaccaa gtattccaaa cagaattgcc atggctttac caattttcga
     7621 ggctattgta gtagacttca catattccat ttttagagct aacaacgctc ttaaaaccct
     7681 gccgccatcc attgggaaag caggaagcat attaaacaga acaagaatta tgtttagtgc
     7741 catgagcttt actaaaaaag agtctgcaga aatatctata ttgataatcg gaattgtagt
     7801 tccagataaa tacagaaatg taaaaattat tgaagctatt gctacattta cgg
//
LOCUS       DBNGSV010000094        13477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1817985, whole genome shotgun sequence.
ACCESSION   DBNGSV010000094 DBNGSV010000000
VERSION     DBNGSV010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13477
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1817985"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="AC159U_03325"
     CDS             <1..466
                     /locus_tag="AC159U_03325"
                     /inference="COORDINATES: protein motif:HMM:NF053168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF8082 domain-containing protein"
                     /protein_id="HIR3336118.1"
                     /translation="EIVGLRCGAFMGNKAMEDLINTEFLSLRFIDSKHLIKSDIPAQI
                     QSIVDRLKLIDSDNEVINKNISQIRSSDSDLQISDMYKFSEEVKALLTDYLGPAASFI
                     FDETKQNVLTGESLTSEQLTQFLIFLAGNINDGADSKKFLDIANNLVKKYSK"
     gene            493..1038
                     /locus_tag="AC159U_03330"
     CDS             493..1038
                     /locus_tag="AC159U_03330"
                     /inference="COORDINATES: protein motif:HMM:NF023273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tll0287-like domain-containing protein"
                     /protein_id="HIR3336119.1"
                     /translation="MATNQTGNTQGIYRGSLLSKFNISLLLLYLFVIIATVPIIYTVT
                     KNQTESAAEKELSILVDFVKSLRTYIAEDVRPTLLEKNIILAPAVSSTVATGRVAGRF
                     LEKQEGYYIKIASDNPLNSANTAIGLEETLLQRFRDDKDLKEITEKGVINGKDYLVSS
                     RPSVSKAGCMKCHNTPPNSAA"
     gene            1082..1399
                     /locus_tag="AC159U_03335"
     CDS             1082..1399
                     /locus_tag="AC159U_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3336120.1"
                     /translation="MGEVVGLSVVGVPLSDVNEITLKRSLYIIGFVTILFFIVFVIIN
                     LQVRKNIISPILDITRKAESISRGNMEERIEINRNDEIGNLSRSFELMRRSIVSMIDK
                     IKK"
     gene            complement(1653..2729)
                     /gene="mqnC"
                     /locus_tag="AC159U_03340"
     CDS             complement(1653..2729)
                     /gene="mqnC"
                     /locus_tag="AC159U_03340"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR3336121.1"
                     /translation="MDCPTLSNKVINNERLTEQDGLYMLQNCDMLQLGALANWMRKKF
                     NPEGYVTFVADTNPNYTNVCDTECLFCAFWRPAGANDAYTMTVDEVIDSMRTSFHNGA
                     TTVLLQGGHNPEIGLDYYLDIIKKWKQEIPDLHLHAFSAPEISAIAKYSGIETKEILQ
                     QFWDAGLRTIPGGGAEVLSNRTRKKISPLKIDADQWMKVTREAHEIGFKTTATMMFGH
                     FEEDEDIIEHLERIRQLQDEFGNFLAFIPWTFKPKNTFLERKLPNEIGGERYLRVLAL
                     CRIYLDNFQHVQGSWFTQGKKMGSITMNYGASDMGGTLYDENVLGCAENKLRSTVDEL
                     VYMIKSAGFKPAQRDTFYNIIKEF"
     gene            complement(2765..3367)
                     /locus_tag="AC159U_03345"
     CDS             complement(2765..3367)
                     /locus_tag="AC159U_03345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006067453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3336122.1"
                     /translation="MSKINVLEHRIPEYPVDKLFITRWSPRAMSGEAITKEELMSLFE
                     AARWAPSAFNNQSWRFLYSLKGSETWDTYFGLLAEGNQAWVNNAGALVLILSKTTFDY
                     NGKPTKTHAFDMGAAWGSFALQGSANGLVIHGMQGFDYDRAKTELKIPDSYDIQAMAA
                     VGKPGNPASLPQKLRDMEIPSTRKSLSEIAVQDASLLITD"
     gene            complement(3385..4089)
                     /locus_tag="AC159U_03350"
     CDS             complement(3385..4089)
                     /locus_tag="AC159U_03350"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008127 - quercetin 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3336123.1"
                     /translation="MIQIRKAEDRGSSTIDWLESYHTFSFGQYYDPDNMQFGYLRVIN
                     EDNIKPSGGFGTHPHRDMEIITVMIDGQLAHKDSMGNGSVIKKGDIQKMTAGSGIMHS
                     EFNASDTKDAHLLQIWITPNKMGLDPGYEQISLDNLGSFEQIKLIASANADTEKGIIK
                     INQNIDLFIGNLASGKKVEFISVESNKIWVQMISGELSINNIDMKSGDGAAITDNSKL
                     EIKSKKESQFLIFEMK"
     gene            complement(4113..4565)
                     /locus_tag="AC159U_03355"
     CDS             complement(4113..4565)
                     /locus_tag="AC159U_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3336124.1"
                     /translation="MATKYKGTRREVGSLNAFINLLRAAGSIEGRLSKNLGGSDLTTS
                     QFGVLEALFHLGPLNQKQLGEKILKSGGNITKVVDNLEKKGLVTREKNLKDRRFYNIA
                     LTQKGTDIIKSIFPLHVKEIVKEFSILTETELSELRKLTRCLGKQRRD"
     gene            4790..5206
                     /locus_tag="AC159U_03360"
     CDS             4790..5206
                     /locus_tag="AC159U_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336125.1"
                     /translation="MDKISEDLFQYAFEVTESTIESFKLEEKDLIVIFSSLNVHKYFG
                     KIDFGIEKSYEKNAQMIIRDAVVYGQEPDFPSKVSDFSFVEDGSIYRNYIDYPFTAKG
                     DLQFVITFYTGDKVTVRSNKVFMIINALDSNKHDIQ"
     gene            5220..6641
                     /locus_tag="AC159U_03365"
     CDS             5220..6641
                     /locus_tag="AC159U_03365"
                     /inference="COORDINATES: protein motif:HMM:NF040005.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3336126.1"
                     /translation="MTKSVLVLLIFGLFLSFSAAHSEQTETTEFSGIYSDNKRVGFNK
                     TTYTNNGNILTINEYSKLKMILLGAESDMEITAKYILNNGNLMNFEFKMNSVGLKLET
                     VGQRYGKKLKFQTSTVSGQSEFTQDIVNEPVVISYIYKWLVKQDLTAGKQYNLHIFEP
                     SMLLMGSKLEDIKADIEIMGREKIDLSTGSYNAHKIKVNFNDSESNVWISDKGELLRD
                     QSSLGLVAIKENEDLSGYQNLARVDITEKTAISASKTIDNPRKLDRLEVKISGLSSLS
                     DFNLNDNYRQFLKDDVLLIKTENIDLFKDLDSIPNKQSITEHYLKPTNLIQSDDENII
                     HLSNNIVGKTKNPVDKIRLINSWVYNSLDKKPTISIPNALDVLRTKTGDCNEHSVLFA
                     ALSRAIDVPTKIILGIVYLDGKFYYHAWNEVFLGNWVSVDATLNQLPVDASHIKFLEG
                     DISRSSEIMKLVGKLNLQIIDAS"
     gene            6648..8420
                     /locus_tag="AC159U_03370"
     CDS             6648..8420
                     /locus_tag="AC159U_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336127.1"
                     /translation="MKINRLVLSVPLILLMLASCSTKGVQVSQVNAREKEKQQIDLSK
                     SNYQAYYHFTLSRLHFFRRDFRKALSEARIAEQIDTESAPLKYNLAIVLMTLNRFTEA
                     LIMLEESIQLNPDYEPSHKILGRIYASSTDPAKRREALEKLNKSAEYDPKDSETHMFL
                     GIIQSEQKNYDQAIDHFKKFVQLSPRDERGYYFLGRIYFQTNQLEKAEKNFRRAVDLN
                     PDYVSALIDLALIYEKRGKLTESEGLYRHMVSKFPNNINVFMRYGNFLYRTNRKSDAS
                     KQYEIAEKLDTDNTDLKLRLVLLYIDSKKYEKAIEDLKLILLGDPENERAKYYLALSY
                     VETDNFEQALDLLNSISAESKLYNDVLIQKSYLYEKQGDVDKSIEIIEQAYASNTDNE
                     PIANYLGNLYRKNKEYKKAILHYKDYLENHPDSEEIVYSLGVTYYLGGEEQKSISTMK
                     EIIKNNPEHVDALNFVGYSYAEMGKNLDEAEMLVKKALELSPNKGYIIDSLGWIYYKK
                     GKLNKALELLLTAARLTSNDPEVMEHIGDVYTDKGELIKALDYYKKGFEIADGVNEEI
                     ADKLKTKVERTELKINELKANKAI"
     gene            8392..9177
                     /locus_tag="AC159U_03375"
     CDS             8392..9177
                     /locus_tag="AC159U_03375"
                     /inference="COORDINATES: protein motif:HMM:NF025490.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HIR3336128.1"
                     /translation="MNSKPIKQYRISYLFILVFIISIFSCAKPGPVIIDTGINEFAKL
                     DISEILSLLKEQEEKIYSVRGLALARLKSPRDKVSFKQVTIVQAPDKLRLEALAPLGR
                     TELAVISDGNKVLLRFPKDDVLYNQIEAFNFSVFYPNIPVPINIYHLSNFALGRLPVD
                     LFEGEYDVEVNGQDNELILNSTSSDDMLWVDFRSLRITKASFMLEDNQKVVVQYDDFI
                     PLEEGYFPKEIELNMTDYSIYIKYDPDVEINQNIRQSLFEIDS"
     gene            9214..9498
                     /locus_tag="AC159U_03380"
     CDS             9214..9498
                     /locus_tag="AC159U_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3336129.1"
                     /translation="MQKNIQLSKTFTIKNKLGLHARAAATLVKTTSQFESDIKIIKDG
                     IEADAKSILGILSFAASKGTHITILTEGEDAEAAMQTIEELIESGFGEGI"
     gene            complement(9495..10448)
                     /locus_tag="AC159U_03385"
     CDS             complement(9495..10448)
                     /locus_tag="AC159U_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3336130.1"
                     /translation="MKIIIPGGSGQVGTLLARAFIKDGHDVTILSRNPELCEWRTVRW
                     DGKTLGKLAEELEDADVVINMAGRSVNCRYNKENRDLIMNSRVDSTRVIGKAIANCKN
                     PPRVWLQASTATIYEDSYDRPNDEFTGVIGGNEPHTPDTWKFSIDVATAWEKILDEAD
                     TPNTRKVKLRSAIILSPDRGGIFDTILQLVRFGLGGKVASGRQMMSWVHYKDFINAIY
                     WIIENEDISDMINICSPNPLPNSEFMKELRKAWGIRIGLPASKLMIEIGTFFLRTKSE
                     LVLKSRYVVPKILQDRGFEFQYQKWNEAAENLCMEWKNQNC"
     gene            complement(10465..10872)
                     /locus_tag="AC159U_03390"
     CDS             complement(10465..10872)
                     /locus_tag="AC159U_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336131.1"
                     /translation="MFTLNNLVLAAGLIQLLILVTASLVPKVTDWKRNLEGSDPFFKS
                     LVWTYGFFIFLIVASIALFSLFKTDLLTDGNATGKYVCGFISVFWGARLFIQFFIFKT
                     PEFLKTGAMKFGYNALTLAFMYLTITYGLVAFT"
     gene            complement(10874..11767)
                     /locus_tag="AC159U_03395"
     CDS             complement(10874..11767)
                     /locus_tag="AC159U_03395"
                     /inference="COORDINATES: protein motif:HMM:NF025185.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HIR3336132.1"
                     /translation="MTEYNKINNLYKFLPASVFVTLGLITKPYVNEWQFMWIFAFAIF
                     FSCKWITLIDALNVIPNASRLRAVLYLFFWVGMDARSFLSGTKIPFISHKIWLEALIK
                     LLTGLFLVYVVAEQVYPYSHFLGGWTGLAGLGLIIQFGSFHLLALIWNKLGVNVKPII
                     NYPFCSVSLTDFWSNRWNLAYRDLSRTYILRPLSPVIGVRYAAILVFIVSGLVHELVI
                     SFPANGGYGLPTLYFIIQTVGVLIEKSALGKILGLENKWRGRIYTLIFVFGPINLLFH
                     NVFIQRVIIPFLYQINSIGGV"
     gene            complement(11816..12355)
                     /locus_tag="AC159U_03400"
     CDS             complement(11816..12355)
                     /locus_tag="AC159U_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR3336133.1"
                     /translation="MEIKSMTPVMKKFILHWGELGTRWGINRTVAQIHALLYLSPKAL
                     NAEEIAETLSIARSNVSTSLKELQSWGVVRVIHEFGDRKDYFESEKDVWEMARRVLDE
                     RKKREIDPTLTILRECVMDSGNKTQGDEYANQKLTEMLDYFEQITAWYDQMSTMSTDN
                     VKRLIKLGSGVKKVLGKVT"
     gene            12514..13377
                     /locus_tag="AC159U_03405"
     CDS             12514..13377
                     /locus_tag="AC159U_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336134.1"
                     /translation="MTKKYIMFLVVFTITFGFSSVLRADEKKDKLVDELYTKSGFKEQ
                     LHNLPEVFNLSLNEELASIDKPQGMLKDYYENIDRLLFKAFDVSTLDAEAKSHLNKNI
                     NVEDIGFLLHWLETPVGKKASKLEVRASRAENVKEFTKYIKNLQSNPIPEPRVKLLEE
                     LESHLNTLDLNVEMLMNIQLATAVAMIYSMSSDNHQEISDIQKKIEGTRLFLQAAIEP
                     LIIGSLDFIYHDLNDDELRILIDFYKKEKAKNANKVFSDAFIKAVTKASYDFGQRSAE
                     FIQNRLKQEKT"
BASE COUNT         4300 a         2430 c         2505 g         4242 t
ORIGIN      
        1 tgaaattgta ggacttagat gtggtgcttt tatgggtaat aaggctatgg aagatttgat
       61 caatactgag tttctctcac ttaggttcat agatagcaag caccttatta aatctgatat
      121 ccctgctcag attcagtcta ttgtggaccg tcttaaatta atagattctg ataatgaggt
      181 aattaataaa aatatttctc aaattagaag tagtgatagt gatttgcaaa tttctgatat
      241 gtataagttt tcagaagaag ttaaagcgct tcttacagat tatctgggcc ctgcagcgtc
      301 atttatattt gatgaaacaa agcaaaatgt acttacaggg gaaagtttaa catcggaaca
      361 gttaactcaa tttttgatat ttcttgcggg caatattaat gatggagctg attcgaaaaa
      421 atttctggat atagctaata acttggtaaa aaaatattca aaataaaaaa ttaatattca
      481 tggagggtaa atatggctac taatcaaact ggaaatacac aaggtatata cagagggagt
      541 ctattaagta aattcaatat atcacttctt cttctatacc tgtttgttat catagcaact
      601 gttccgatta tttatacagt tacaaaaaat caaactgaga gtgctgctga aaaggaactc
      661 tcgatcctag tcgattttgt taaatcactc agaacatata ttgctgaaga tgtaagaccc
      721 acattgctgg aaaaaaatat aatattagcg cctgcagtat ccagtacagt ggcaacgggt
      781 agggttgcag gaaggttttt ggaaaaacag gaaggctact acattaaaat tgcttctgat
      841 aatccattga attcggcaaa tactgcaata gggcttgaag aaacactgct gcagaggttt
      901 agagatgata aagatcttaa agaaattaca gaaaaagggg taattaatgg taaagactat
      961 ctagtatcct caagaccaag tgtaagtaaa gctgggtgta tgaaatgtca taacaccccc
     1021 ccaaacagcg ccgcctgaga tagttaatgc atacggctat agaagtggtt ataactataa
     1081 aatgggagaa gtagtaggac ttagtgttgt gggtgtgcca cttagtgatg ttaatgaaat
     1141 tacattaaaa agaagtctat atattatagg atttgtaaca atattgtttt ttattgtttt
     1201 tgtgattata aatttacagg taagaaagaa catcatttca ccaatattag atattactag
     1261 aaaagctgaa tctatcagta gaggtaatat ggaagaaagg atagaaatta atagaaatga
     1321 tgagattggt aatctgtcaa gatcgtttga actgatgaga agaagtatcg tgtctatgat
     1381 agacaagatt aaaaaataat ctataaatta attcactgtt aatattgagg ctgttcgaat
     1441 attaagtttg gacagcctct aaaattttct ataatagaaa aaaccgcaag gtaaactccc
     1501 ggtatctgtt tttaatctca agattagtag aagttaaaga gcattaacct aaagagggtt
     1561 atatatagtt aaacatttca cgattaatgg gctgttaact aatagtttga gatatttagc
     1621 ttgagagtgt tagaaaatgg ataaacaaga tattaaaact cttttattat attataaaag
     1681 gtgtctcttt gtgcaggttt aaatccggcg ctttttatca tatataccag ttcatcaact
     1741 gtagaacgta gtttattctc tgcgcagccg agtacgtttt catcgtaaag cgtgccgccc
     1801 atgtcgcttg ctccatagtt catggttatt gaacccattt ttttgccctg agtgaaccat
     1861 gagccctgaa catgctgaaa attatccaga taaatcctgc atagcgctaa aactcgcagg
     1921 tatctttcac cacctatctc atttggaagt ttccgttcca aaaacgtgtt ctttggttta
     1981 aatgtccacg gaataaaggc caggaaattg ccaaactcgt cctgaagctg cctaatcctc
     2041 tccagatgtt caataatatc ctcatcctct tcaaaatgac caaacatcat cgttgctgta
     2101 gttttaaagc ctatttcatg cgcttctctg gtgaccttca tccactgatc ggcatcgatc
     2161 tttagtgggc ttatcttttt tcttgtcctg ttgctaagaa cctctgctcc accaccgggg
     2221 attgtcctta gccccgcatc ccaaaactgc tgtagaattt cttttgtttc aatgcctgaa
     2281 tacttggcta ttgcgctaat ctccggagcc gagaaagcat gcaggtgaag atcggggatt
     2341 tcctgtttcc atttctttat aatatcgaga taataatcaa gccctatctc cgggttatgc
     2401 ccgccctgaa gcagcaccgt cgttgccccg ttatgaaaag aggttctcat cgagtcaatg
     2461 acctcatcca ccgtcatggt ataagcatca tttgcccccg ctggtctcca gaatgcgcaa
     2521 aaaagacact ctgtatcaca tacgtttgtg taattagggt tagtatcagc cacgaaggta
     2581 acataaccct cgggattaaa cttcttacgc atccagttgg caagagcgcc aagctggagc
     2641 atatcgcagt tttgaagcat atacaggccg tcttgttcgg ttagcctttc attattaatg
     2701 accttgtttg aaagagttgg gcaatccata gcggcaatta ttgaacccaa cagtttagca
     2761 ggtgtcaatc agttattaaa agagatgcat cttgaactgc aatttcactc aagctctttc
     2821 tggtcgatgg tatttccata tctcttaatt tttgcggaag gcttgcggga ttgccgggtt
     2881 ttcccactgc agccattgcc tgtatatcat aactatctgg tattttaagc tctgttttag
     2941 ctctatcata atcaaacccc tgcataccgt gaattacaag accatttgct gagccctgta
     3001 gtgcaaaact tccccatgct gcacccatat caaacgcatg ggtttttgtt ggctttccat
     3061 tataatcaaa tgtggtcttt gataatatca aaactaaagc ccctgcattg ttgacccagg
     3121 cctgattacc ttctgcgaga agcccgaaat atgtatccca ggtttctgat cccttaagtg
     3181 aatacaaaaa tctccatgac tgattattaa atgcagaagg tgcccacctt gcagcctcaa
     3241 aaagtgacat tagctcttct tttgttattg cttcaccaga cattgctctt ggtgaccagc
     3301 gagtgataaa taatttatcc accgggtatt ccggtattct gtgctctaat acgtttattt
     3361 tgctcatgct ctatctcctt tgaattattt catttcaaaa attaaaaact gtgattcttt
     3421 ttttgattta atctctaatt ttgaattatc tgtaatagca gcaccgtctc cgcttttcat
     3481 atctatattg ttaattgata attctccgct tatcatttga acccatattt tgttagactc
     3541 aacggagatg aattcgactt ttttaccgct tgccaaatta ccgataaaca gatcgatatt
     3601 ctggtttatt ttgattatac ctttttcggt atctgcgttt gctgaagcta ttagcttgat
     3661 ttgttcaaaa ctaccaaggt tatccaaaga aatctgctca tatccgggat caagccccat
     3721 cttgtttgga gtaatccaga tctgaagcag atgagcatct tttgtgtctg atgcattaaa
     3781 ttcactgtgc ataatccccg agcccgctgt cattttttga atatctccct tttttataac
     3841 agatccgttt cccatgctgt ctttgtgggc aagctgtccg tctatcataa cggtaatgat
     3901 ctccatgtct cggtgcggat gagttccaaa gcctccggag ggttttatat tgtcctcgtt
     3961 aattactctt agatagccaa actgcatatt gtccgggtca taatattgtc caaatgaaaa
     4021 tgtgtgataa ctttccagcc agtcaatagt agaacttcct ctatcctcag cctttcttat
     4081 ctgtatcatg ataaatcccc cctattaaat atttagtccc ttctttgttt gcctaaacat
     4141 cttgttagtt tcctaagctc tgagagttca gtttctgtaa gaatgctaaa ttcttttact
     4201 atttccttta catgcaacgg gaatattgat tttataatat ctgttccctt ttgcgtaagc
     4261 gcaatgttgt aaaaacgcct gtccttcaga tttttttctc ttgtcacaag gccctttttc
     4321 tcaaggttat cgactacttt ggtgatattg cctcctgatt ttaatatttt ttctcctaac
     4381 tgcttctggt ttaacgggcc caggtgaaaa agtgcttcga gcactccgaa ctgacttgtt
     4441 gtaagatcag atcctccaag atttttggaa agtctgcctt caatggagcc tgcggctcta
     4501 agcagattaa taaatgcgtt tagcgatcca acttctcttc ttgtcccttt gtatttagtt
     4561 gccataattt attaaactca ataatgcatt gatattaaat agtttaatat taaaataata
     4621 tataaataca atatgtcaat aagtagcttg ccaattaaaa tagaatatta ttttcatgtc
     4681 cggtatcagc tatggatttt attctagatc actatatatt tagacataag tattagtttt
     4741 gatgcgtaac atatcttctg attattagtt tgatataaca ttaatattca tggacaagat
     4801 aagtgaagat ctttttcagt atgcttttga ggttaccgaa tcgacaatag agtcattcaa
     4861 attggaagag aaggatttaa ttgtcatttt cagttcgtta aatgttcata aatattttgg
     4921 taaaatcgat ttcgggatag aaaagagtta cgaaaaaaat gctcaaatga tcataaggga
     4981 tgccgtggtt tatgggcagg aaccagactt tccctccaaa gtatcagact tttcttttgt
     5041 agaggatggg agtatatata gaaattatat tgactaccct tttacagcaa agggggatct
     5101 gcagtttgtg attacatttt atacgggcga taaggttacc gtaaggtcaa acaaggtatt
     5161 tatgataata aatgccctag acagtaataa acatgatata cagtaggtta taattaacta
     5221 tgacaaagtc agtgttagta cttttaatat ttggactatt cttaagtttc tctgctgcgc
     5281 attctgagca gactgagacc acagagtttt caggaattta ttcagataat aaaagagtag
     5341 gttttaataa gacaacatat actaataacg gaaatatttt aaccataaat gaatactcta
     5401 agctcaagat gatactgcta ggcgctgaaa gcgatatgga gattactgca aaatatatct
     5461 taaataatgg caatttaatg aattttgagt tcaaaatgaa ctcggtcggg ctcaagctgg
     5521 aaactgtagg ccaaagatac gggaagaagc taaaatttca aacatccacc gtatcaggac
     5581 aatcggaatt tactcaggat atagttaatg agcctgtagt aatttcttat atttataaat
     5641 ggttagtaaa acaggatctt acagccggca aacaatataa tctacatatt tttgaacctt
     5701 caatgctcct gatggggtct aaactggagg atataaaagc cgatattgag attatgggaa
     5761 gggaaaaaat tgatctatct accggtagtt ataacgcaca caaaataaag gttaatttta
     5821 atgattctga atcaaatgta tggatttctg ataaagggga gcttcttagg gaccaatctt
     5881 cacttggact cgttgcgata aaggaaaatg aggatttatc tggctatcaa aatcttgcaa
     5941 gggttgatat tacagaaaaa actgcaatat ctgcatctaa aactattgat aatccaagga
     6001 aacttgatag attggaggta aagatttccg gccttagttc acttagtgat ttcaatctta
     6061 atgataatta cagacagttc ttaaaggatg atgtgctcct tataaagact gaaaatatag
     6121 acttatttaa agatttagat tctataccaa ataaacaatc cattacagaa cactacctga
     6181 aacctacaaa ccttattcag agtgatgatg aaaatattat acatctttcc aataatatag
     6241 taggcaagac caaaaacccg gttgataaga taaggctaat taattcatgg gtgtataaca
     6301 gcctggataa aaaaccaacc atcagtattc ccaatgcgct tgatgtcctg cgtacaaaaa
     6361 cgggtgactg caatgaacac tctgtacttt ttgctgcact ttcaagagct atagatgttc
     6421 caacaaaaat aatattgggt attgtatact tggatggtaa gttttattac catgcctgga
     6481 acgaggtatt tttgggtaat tgggtttctg tggatgctac attaaatcag ctgcctgtag
     6541 atgcttcgca cattaagttt ttagagggtg atataagcag aagttcggag attatgaaac
     6601 tagtgggtaa acttaattta cagataatag atgcatctta aaatcttatg aaaataaata
     6661 gacttgtttt atcggtaccg cttatattat tgatgcttgc ctcgtgctca acaaaaggag
     6721 ttcaggtctc acaagtaaat gcccgggaaa aagagaagca acaaatagat ctatccaaga
     6781 gcaactacca ggcctattac cattttacac tttcaagact gcactttttc agaagagatt
     6841 ttagaaaagc gcttagtgaa gcaagaattg ccgagcagat agataccgaa tcagcaccgc
     6901 ttaaatacaa tcttgcaata gttcttatga ccctgaaccg ttttactgaa gcattaatta
     6961 tgcttgaaga aagcattcag ctaaatccag actatgaacc ctcgcataaa atactcggta
     7021 ggatctacgc atcgtccaca gaccccgcaa aaaggcgtga agctttggaa aaactaaata
     7081 aatcagccga gtatgatcct aaggattcag agacccatat gtttttggga ataattcagt
     7141 ccgagcaaaa aaactatgat caggctatag atcatttcaa aaaatttgta cagctttccc
     7201 caagggacga gaggggttac tattttttgg gaaggattta ttttcagaca aaccagcttg
     7261 aaaaagctga aaagaacttc aggcgtgctg ttgatcttaa ccccgactat gtatcggccc
     7321 ttattgatct tgcactgatt tatgaaaaaa gaggtaagct tactgaatcg gaagggcttt
     7381 acaggcatat ggtaagcaag tttccaaaca atatcaatgt gtttatgcgt tatggaaatt
     7441 tcctttacag aacaaacagg aaatcagatg cttcaaagca atacgagata gccgagaaac
     7501 tcgataccga caacaccgat ttaaaactaa ggctggtact gctgtatatt gacagtaaaa
     7561 aatatgaaaa agccattgag gatcttaaat tgatactgct tggtgaccct gaaaatgaaa
     7621 gggctaaata ttatcttgca ctaagttatg tagaaaccga taacttcgag caggcactcg
     7681 atttacttaa ttccataagt gctgaatcaa aattatataa cgatgtgctt atacaaaaat
     7741 catatctgta tgagaaacag ggagatgttg ataagtcaat tgaaataata gaacaggcat
     7801 atgcatcgaa tactgataat gaacccatag ccaattatct tggaaatctg tataggaaaa
     7861 ataaagaata caaaaaagct atattacatt ataaagatta tctggaaaat catccagata
     7921 gtgaagaaat agtttattcg ctgggtgtta catactatct tggcggagaa gaacagaaat
     7981 ccatttctac tatgaaagaa ataatcaaga ataatccgga gcacgttgat gcgcttaatt
     8041 tcgtaggtta ttcatatgcc gagatgggaa agaatcttga cgaggcagag atgctcgtaa
     8101 aaaaggctct ggagctttca cctaataaag gatacataat agacagtctt ggttggattt
     8161 actataaaaa aggtaagctg aataaagcgc tggagctatt gcttacggct gcaaggctta
     8221 catcaaacga ccctgaggtt atggagcaca tcggcgatgt atataccgat aagggcgagc
     8281 ttattaaggc tttggattac tataagaagg ggtttgaaat agcagacggt gtaaatgaag
     8341 aaattgcgga caaactaaaa accaaggtag agcgtactga gcttaaaatt aatgaactca
     8401 aagccaataa agcaatatag aatttcttat ctgtttattc tagtatttat catttctatt
     8461 ttttcctgcg ccaagccggg tcctgtaata atagataccg gtataaatga atttgcaaag
     8521 ctggacattt ctgaaatctt atcacttcta aaagaacagg aagagaaaat atattctgtt
     8581 agggggctag cgcttgcaag actgaaatcg ccgagggata aagtatcttt taagcaggtt
     8641 actattgtac aggctcctga taaattacgg cttgaagctt tggcaccttt gggaagaaca
     8701 gagcttgctg taatctcaga tggcaataaa gtgttactcc gatttccaaa ggatgatgta
     8761 ttatacaatc agattgaagc atttaatttt tcagtatttt atcccaatat tcctgttcct
     8821 ataaatatat atcacctttc taattttgca ctgggaaggc ttcctgtcga tctctttgaa
     8881 ggtgaatatg atgttgaggt aaacgggcag gataacgaat taatccttaa ttctacaagc
     8941 tcagatgata tgctctgggt tgattttcgt tctttaagga taaccaaggc ttcattcatg
     9001 ctcgaggata atcaaaaggt tgttgtacaa tatgatgatt ttattcctct ggaagaaggt
     9061 tattttccaa aggagattga gctcaatatg actgattatt caatctacat aaaatatgat
     9121 ccggatgttg agataaatca gaatatcagg caaagtcttt tcgaaattga ctcatagaaa
     9181 ataataaatc tgctgttggt aagaattaga taattgcaaa agaatattca gctttcaaaa
     9241 acttttacta ttaagaataa attgggcctg catgcgcgcg ctgctgctac tcttgtaaag
     9301 accacaagtc agttcgaatc agatattaaa atcataaaag acgggataga ggcagatgca
     9361 aagagtatac tcggcatact ctcatttgca gcttctaaag gcacacatat caccatttta
     9421 acagaaggtg aggatgcaga ggctgctatg caaactattg aagaactcat tgaatctggt
     9481 tttggtgaag gcatctagca attttgattt ttccattcca tacacaaatt ctcagctgct
     9541 tcattccact tctgatattg aaattcgaac cctctatcct gaagaatctt tggcacaaca
     9601 tatctgcttt taagcacaag ctctgattta gttcttaaaa agaaagtacc tatttctatc
     9661 ataagttttg atgcaggaag accaattctg ataccccagg cttttctaag ctctttcatg
     9721 aattcactat tcggcagtgg atttggagag cagatattaa tcatatccga tatatcttca
     9781 ttctcaatta tccagtagat ggcgtttatg aaatctttat aatgaaccca tgacatcatt
     9841 tgcctgccgc ttgctacttt gcctccaaga ccaaacctca ccagttggag tatggtatcg
     9901 aaaattcctc ctctgtcggg gctaagtata atagcagatc gtagttttac tttgcgtgtg
     9961 ttgggtgtat ctgcctcatc gaggattttc tcccatgcgg ttgcaacatc tatactaaac
    10021 ttccatgtat caggagtgtg tggttcattt cctccaatca cacctgtgaa ctcatcattc
    10081 ggtctatcat aactgtcttc ataaatggtt gcagtgcttg cctgtagcca taccctgggt
    10141 ggattcttac agttggcaat tgccttgccg attacacggg tggaatcaac acgggaattc
    10201 attatcaggt cacgattttc cttgttatat ctacagttta cgcttctgcc ggccatgtta
    10261 attacaacat cggcatcttc tagctcttca gccaatttgc ccagagtttt cccatcccat
    10321 ctaactgtgc gccattcaca aagttcagga ttacgactaa gtatggtgac atcgtgtccg
    10381 tcttttataa acgctcttgc aagcagagta cccacctgac cagagcctcc ggggattatg
    10441 attttcattt ttctttcctc aaatttatgt aaatgccaca agcccgtatg ttattgtcag
    10501 atacataaac gccagggtta gtgcgttgta tccaaacttc atagcaccgg ttttaagaaa
    10561 ctccggagtc ttaaaaatga agaactgtat gaaaagacgc gcaccccaga atacggaaat
    10621 aaaaccgcag acatattttc ctgttgcgtt cccgtcggtg agaaggtctg ttttaaaaag
    10681 agaaaacaac gctatgcttg cgacaatgag aaagataaaa aacccgtatg tccagacaag
    10741 gctcttgaaa aacggatcac tgccctcaag attccttttc caatctgtta cctttggcac
    10801 tagcgaggct gtgaccaata ttaatagctg aattaaacct gctgctaaga ccagattgtt
    10861 tagagtaaac atactaaaca ccccctatag aatttatttg atataaaaac ggaataatta
    10921 ctctttgaat gaaaacgtta tggaacaaaa gatttatggg gccgaaaaca aaaatgagtg
    10981 tgtatatgcg gcctctccat ttattctcta aacctaaaat ctttcccaga gctgattttt
    11041 ctatgagcac acccacagtc tgaataatga aatacagtgt cggcaggcca tagcctccgt
    11101 ttgcaggaaa tgagattact aactcgtgta cgaggcctga gactatgaat acaagtattg
    11161 ctgcatacct aactccgatt actggtgaaa gcggccttaa tatgtaggtt ctggataagt
    11221 ctctgtatgc aaggttccac ctattgctcc aaaaatctgt aagtgaaaca gagcagaacg
    11281 ggtaatttat aattggtttt acatttacgc caagtttatt ccagatgagt gcaagcaggt
    11341 gaaacgatcc gaactgaata ataagcccaa gaccagcaag ccctgtccag ccgcccagga
    11401 aatgactata aggataaacc tgctctgcaa ctacataaac caaaaacagg ccggttagta
    11461 gtttaattaa tgcttcaagc catattttat gactaataaa aggtattttg gttcctgaga
    11521 gaaaactcct ggcgtccatt ccaacccaga aaaaaaggta taagactgct cttagcctac
    11581 ttgcatttgg aatcacgttc agagcatcaa tgagagtgat ccatttgcat gaaaaaaata
    11641 ttgcaaaggc aaagatccac ataaactgcc attcgtttac gtagggtttt gtaataagcc
    11701 ctaaagttac aaaaacagat gctggcagga atttatatag attgtttatt ttgttatatt
    11761 cagtcatttc tgtaatcacc gaaattacaa gttaaaaaat atatttgaat ttattctatg
    11821 ttacttttcc aagcaccttc tttactccgc ttccaagttt tataagtctt tttacgttat
    11881 ctgtagacat ggtgctcatc tgatcatacc atgccgtaat ctgctcaaag taatccagca
    11941 tctcagttag tttctgatta gcatattcgt caccttgtgt tttatttcct gagtccatta
    12001 cacactctct taaaattgta agggtagggt ctatctcacg cttctttctt tcatcaagta
    12061 ctcttcttgc catctcccaa acgtcttttt ccgattcaaa gtaatccttt ctgtctccaa
    12121 attcatgtat tactcgcaca actccccagc tctgaagctc cttaaggctt gtgctcacgt
    12181 ttgatctggc aatagaaaga gtctcggcaa tttcctctgc attaagcgct ttgggagata
    12241 agtataacag tgcatgtatc tgcgccactg tcctgttaat tccccaccgg gttccaagct
    12301 caccccaatg cagtataaat tttttcatta ccggagtcat agattttatt tccatattta
    12361 cttatttgtt ctcatatttc tgtatgtaca gaaaatacag gaaattggat ttgatgtcaa
    12421 gaagtttttg ataaccaact aaataagttg tctaatttat ataaaaggtg gataatagta
    12481 taaataatat tgatccttat aaatgggggc aagatgacta aaaaatatat tatgttttta
    12541 gttgtattta ctataacatt tggtttcagt tctgttttaa gagcagatga gaaaaaagat
    12601 aagctagtcg atgaactata tacaaaatca gggtttaaag aacagttaca caatctaccc
    12661 gaagtgttta atcttagtct taatgaggaa ttagcatcta tagataaacc tcagggtatg
    12721 ctgaaagatt attatgaaaa tattgataga cttttgttta aggcttttga tgtgtcaaca
    12781 ttagatgctg aagctaaatc tcacttaaat aaaaatatta atgttgagga tataggtttt
    12841 ttattgcatt ggcttgaaac cccggttggt aaaaaagctt ctaaacttga ggttagggca
    12901 tcaagagctg agaatgttaa agaatttacc aagtatatta aaaatttaca atctaatccg
    12961 attccagagc caagggttaa gttattagaa gagcttgaaa gtcatttgaa tacactggat
    13021 ttgaatgtgg agatgttgat gaatatacaa ttagctactg ctgtggccat gatttattct
    13081 atgtcttcgg ataatcatca agaaatttct gacatacaga aaaaaataga aggtacgaga
    13141 ctatttcttc aagctgctat agaaccttta attataggtt cattggattt tatatatcat
    13201 gatctgaatg atgatgaact aagaattttg attgatttct acaaaaaaga gaaggcaaaa
    13261 aatgctaata aagtttttag cgatgcattt ataaaagctg ttactaaagc tagctatgat
    13321 ttcggacagc gatccgctga gtttattcag aatcgtctaa aacaagaaaa gacttaaaga
    13381 attatttata gagactaaga ttaagtctct aaatatataa tctttattta acatctctaa
    13441 ttttttgtgt aattcttaac agatatgtta attcaaa
//
LOCUS       DBNGSV010000095        10219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_571185, whole genome shotgun sequence.
ACCESSION   DBNGSV010000095 DBNGSV010000000
VERSION     DBNGSV010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_571185"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1140
                     /locus_tag="AC159U_03410"
     CDS             322..1140
                     /locus_tag="AC159U_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3336135.1"
                     /translation="MQIRVGYELIYDCPQPTPMILTLHIHFTRDSDIIVPDHLIIRPS
                     IHITTYRDAFGNRCSRIVAPKGEIQLTADAVVNDTGEPDLVAASAHQHPVQDLPEETL
                     VFLLGSRYCETDLLSDVAWGLFDKSPTGWGRVQAICDFVHQHITYGYEHARATKTAWE
                     AFNERTGVCRDYTHLAIALCRCMNIPARYCTGYLSDIGILPPYGPGDFAAWFEAYLGG
                     HWYTFDARYNVPCIGRVLIARGRDAADVAISHTFGPNTLKSFKVWTEEVSTGLT"
     gene            1545..2000
                     /locus_tag="AC159U_03415"
     CDS             1545..2000
                     /locus_tag="AC159U_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336136.1"
                     /translation="MSRYFVLILLSAFITGISLDYTHAVKGNRVTIDDDCSCSDRDRR
                     HRLFTSIDDSDCTNTSQSNPTVWIQLRQAPSDTDFHIEVIHTDSDNSIDIFPDFISCG
                     DNLYYFQLDTGFVSDLSHFNGNGRYLVGIYDELFFSGFIGSARYVVDIP"
     gene            2409..2831
                     /locus_tag="AC159U_03420"
     CDS             2409..2831
                     /locus_tag="AC159U_03420"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3336137.1"
                     /translation="MANKTLLTKRVSFSASHRYWNKNWSDEKNNEVFGKCTSPHGHGH
                     NYVLETTVQGEIDPETGMIINLYDLKPIILDALEDFDHKFLNEDNPAFTDLIPTTENI
                     AKVLWDVISERLESHSCRLHRIRLYETPELYVDYWRDK"
     gene            2828..3277
                     /locus_tag="AC159U_03425"
     CDS             2828..3277
                     /locus_tag="AC159U_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HIR3336138.1"
                     /translation="MSSVNIPEHCCLTKIYSFSAGHRLSLDRLTPEENSRVFGKCSNP
                     RGHGHDYYLEVRVEGQINPDTGMIVHLNELDSSVNNIISDLDHKRLDIEVDYFTSNQS
                     TGENIVRYFWGKLKSNVEDCNLVYLKLWETENNYFEYFEEKDIKYAN"
     gene            3267..3830
                     /gene="folE"
                     /locus_tag="AC159U_03430"
     CDS             3267..3830
                     /gene="folE"
                     /locus_tag="AC159U_03430"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3336139.1"
                     /translation="MPIEDLIKDMLVEIGEDPERQGLADTPSRVARMYKDELTSGYTK
                     CPHKVINDAIFDEKYDEMVVVKDVEFYSLCEHHMLPFFGKCHVAYVPDGKIIGLSKIP
                     RLIEVYSRRLQVQERMTMQIADILNESLSPHGVAVVVEAYHLCMAMRGVKKARANMLT
                     SSMLGVFKKDERTRAEFLSLIGQSNIK"
     gene            3834..4538
                     /locus_tag="AC159U_03435"
     CDS             3834..4538
                     /locus_tag="AC159U_03435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3336140.1"
                     /translation="MKKVVLITGAGKGIGKETALTFARAGFIIAACGRTASDLKTLKR
                     QIEKAGSAAVTSVCDISKESQVKRFIAKAVKITGRIDAVVNNAAVASIGSIYDTDLKD
                     WQNTIDTNLTGAFLITKHSLEHMEKGGHIFNIGSNASKIGFPNWSAYCASKFGLLGFS
                     NSIREELRSKGIKVSAILPGPTKTPLWGKLGKKKWDKSKMMEPAGVAEMILHVYNQPA
                     DVQTEEVYIIPSSGGL"
     gene            4649..5980
                     /gene="hemA"
                     /locus_tag="AC159U_03440"
     CDS             4649..5980
                     /gene="hemA"
                     /locus_tag="AC159U_03440"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3336141.1"
                     /translation="MHNIFTLGISYKDTPVELREKLSFSGNDTERVLSEILTDTVLEE
                     CIIISTCNRIEFYAIAEDIELCSDQIKQFVCNYNKISSDKLNSHFYLYTGAQAVRHLY
                     RVSSSLDSMVVGEPQILGQVKDAYKTATKADSTGLILNKLFHSAFFTAKKVRSETGIG
                     SQAVSISYAAVELAKRIFDDFSERTAVLIGSGEMGELAAKNLINVGINELIIASRNIN
                     NARVLAQNLGGKAVGMEEVYYHINHADIIITATDSIDYIIKPHHLNEALGVRKNEPIF
                     LIDIAVPRDIDPRIHDFENVYLYDIDDLAGVLEENIESRKKSAKRAEEIINERVDRFS
                     HWIEQLKVFPAIVELKNRFELIKNLETDKALRKLENSQGDEKEIIEKMASSLISKLLH
                     SPLSNLKKESSGYSGLIYVDAITKLFDLQTKLTLVDDESDEEPSKDWNERQ"
     gene            5940..6863
                     /gene="hemC"
                     /locus_tag="AC159U_03445"
     CDS             5940..6863
                     /gene="hemC"
                     /locus_tag="AC159U_03445"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3336142.1"
                     /translation="MMKSLLRIGTRGSKLALWQANFVKDSLQTIFPKLVVYLKIIRTS
                     GDSFTDRPLAEIGGKGLFVKEIEEALLADKVDIAVHSMKDVPYELPPGLEISTFLKRE
                     DPRDALLSNGHVKFADLPPGATVGTGSLRRQVQIQRLRDDLDIKPLRGNVDTRIRKLI
                     DGQYDAIVLASAGLNRLELGSEITEHFSVSDLIPSPGQGIIGIESRIDDKDTREIISR
                     LKDADATCAIGAERNFVKQMGGDCSLPLGCYCEISNDTEIEISGFVASVDGKDLVKDT
                     VSGATHENIKLAEQLAKKIISNGGKEIIESF"
     gene            6872..6979
                     /locus_tag="AC159U_03450"
     CDS             6872..6979
                     /locus_tag="AC159U_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR3336143.1"
                     /translation="MIVPTMGQWGMLLAAIVLGFFAILRLRKIDESEFK"
     gene            7127..7969
                     /locus_tag="AC159U_03455"
     CDS             7127..7969
                     /locus_tag="AC159U_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR3336144.1"
                     /translation="MFSDMLASRIEDVEFCILDVETTGLNPIAGDKICEIAIVKSRDS
                     KTLGTYETLVNPGIKIPISATGIHGIHDEDVSEAPYFLDIAEDIQRFLAGSVIVAHNA
                     RFDLGFLDAELNFTTDISVKNPIVDTLGIARKFYTFPTNNLGVIAKTLGIPTAGYHRA
                     MADVEITKDVLEYFIKDLRRRGIRLNTLKDLVRVQGGGIEIDRPRDSILPKVIEDALI
                     NKAKLQIKYLSPFSDSVKDRVIEPLDVLERRTNTYLIAYCHLRKQKCNFRLDRILEIS
                     TVSS"
     gene            8040..8116
                     /locus_tag="AC159U_03460"
     tRNA            8040..8116
                     /locus_tag="AC159U_03460"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8074..8076,aa:Met,seq:cat)
     gene            8442..>10219
                     /locus_tag="AC159U_03465"
     CDS             8442..>10219
                     /locus_tag="AC159U_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HIR3336145.1"
                     /translation="MSLRKKTVLIVGIASVTIVLVLYSIISRILLDSYRELENQDTKK
                     SVNQVLSKISDELDNLSSTADNWSARNETYNFMLGDSRKYAEENLLNNSLKNQKLNFI
                     IFVDKKGDIFHHKGVDYKSGKDIALPGDLADIISSQSTVVKHAYPSSSTSGFIQTKDS
                     TLMFASRPIINNDLTLPIAGTLIVARFFDSYELKYISESTLQTVRFKGYNDRHEAADC
                     EHAAEIIFHTNKIYVNELDETLIAGYALIKDIFDKPALIVRVDKQRSIFMQGKKTINY
                     FLLALILIGIAFCILIYFFLENFVISKLLKFSDSVNNITSNQDFSSRISFSGKDELSN
                     LGTNINEMLQSLEDKQKIIKTTNDELESRVKERTQELLTANTALEKEIKNRMEKEEEL
                     KVNVQSLSKKNKYEMIIRSVAESVNKTKDLQQIIDNAIKSIHDNIEHATTISLYLVTG
                     NVAQLKGAIGHPQWFIDRVSNIPYPKGFTWKTIIDNTSLLCNDTDLDTAMGQAGKDLG
                     IMSYLSVPLRHGEESVGCLKVDSFQKNSFSSEDQKLLEIVARQIDVAIDNSKADFALK
                     TNIEQLSRKNKHESIIRTVTQSVHQS"
BASE COUNT         3202 a         1943 c         2231 g         2843 t
ORIGIN      
        1 ttctctcgta ttctttgagt attggtatcc atctctttac taccactatc tcatggtatt
       61 tcattaagca cctccttagt tattaatata tactaatttg gttacttaat tactgagcat
      121 ttacaagcgt cccctttttc tcttatcccc ttttttaccc tttttctttc aaagacatgt
      181 ttgacattaa tatgaaattt tatgatacgg tataatatta ttcagacaat ttctatatag
      241 ttacaataca ccgtatactc catcaatgag ttatcgtgct tactccactc atcgttggga
      301 cactacttaa ggaggttaat catgcagata cgagtcggct acgagttaat ctacgactgt
      361 ccgcagccaa cgcctatgat actgacgtta cacatccatt ttacgcgcga ctccgacatc
      421 atcgttcccg atcacctcat catcaggccg tccatccata ttactaccta tcgtgatgcc
      481 ttcggaaaca ggtgcagccg gattgtcgca ccgaagggcg agatccagct tactgccgat
      541 gcggtcgtga acgatacggg tgaaccggat ttggtcgccg cctcggccca tcagcaccca
      601 gtgcaagatc ttcccgagga gaccctggtg ttccttttgg gaagccgata ttgcgaaacc
      661 gatctgttgt ccgatgtggc ttggggccta ttcgacaaat cgcccactgg atggggacgc
      721 gtccaggcca tctgcgattt cgtccatcaa catatcacct atggttacga acatgcccgc
      781 gccacgaaga cagcctggga agctttcaat gagcggaccg gcgtctgccg tgattacacg
      841 catctcgcca ttgcactgtg ccgttgcatg aacattccgg cacgctactg cactggatac
      901 ttgagcgaca taggcatact accgccatac ggcccggggg atttcgccgc ctggtttgag
      961 gcataccttg gcggtcattg gtacaccttt gatgctcggt acaacgtgcc gtgtatcggc
     1021 cgagtgctga tcgcgcgagg tcgagatgcc gctgatgttg cgatcagcca cacgttcggc
     1081 cccaacacgc tgaaaagctt caaggtctgg acagaagagg tgagtacagg acttacctaa
     1141 ccacacaacc ccacaaaacc agatatcctt gcaccgatag aaaaatagta aaaaatagtg
     1201 acagacacta ttttatcgag attcttctaa cttattatac cagagacatt ttatcttaat
     1261 cttaactacg agattgccgc tcatggctta atgaaggcga atatcaccta attaatagtg
     1321 acagacacta tttataaagg ggatatttat tatagataaa ataatagggc catgaagatt
     1381 tacatatatt tagatcacat aacataagac ttgacaatca ttatttgatt atgatcgaat
     1441 aattcgatag tcgaatgtga tacttagatt actcggttat tagtctaact agctataacc
     1501 attgaaggct ggtatagcaa tttaatctta aaggagagtt taaaatgagt agatactttg
     1561 tattgatatt attgtctgca tttattacgg gtatatcctt ggattacaca catgctgtaa
     1621 agggtaaccg agtcacaatt gatgatgact gttcatgttc agaccgtgat agaagacata
     1681 gattattcac atctatagat gacagtgatt gtacaaatac tagtcaatct aatccaacag
     1741 tttggattca actgagacaa gcacctagcg atacagactt tcatatagaa gtaatccata
     1801 cagatagcga taatagtata gatattttcc ctgattttat ttcttgtggt gataatttat
     1861 attattttca gctggatacc ggatttgtct ctgatttatc tcactttaat ggaaatggga
     1921 gatatttagt tggaatatat gacgagttgt tcttttctgg gtttattggt agcgcaagat
     1981 atgtagtaga tattccctga ttaaacgtaa aaaaggggat tctacccttt ttgtaattta
     2041 gacccaaaaa cccataaatc cagggttaaa ccctaaacct ttgggttttt ggggttaagg
     2101 tgtatgactt tgttttatta atagaatagc acgcacttat aagatgtatt tataacactc
     2161 gcatttcgcc tgtcctgagc ctgccgaagg aactgttcga tgcgtttggt tcccttcatt
     2221 ctgaccgtaa tgtagtggat agaagaatct ccgaatccag atttgtcatg ctagagcgta
     2281 tgcgaagcat cttgttttta gattcttcgc cgcggctcag aatgacagta atactgcgaa
     2341 tggaaacaaa acacaccttc catcatacca atcctgtgat aatatatcgg ttttcttaaa
     2401 aacaagagat ggcaaacaag actttactta caaaaagagt gagtttttcg gcttcgcacc
     2461 ggtactggaa caagaactgg tcggatgaga aaaacaacga ggtattcggc aaatgcacaa
     2521 gcccgcacgg gcacgggcat aactatgtgc tcgagaccac cgtgcagggt gaaatcgatc
     2581 ccgaaacggg gatgataatt aacctctacg atctaaagcc cattattctg gatgcgctcg
     2641 aagactttga ccataaattt ttaaatgaag acaaccccgc gtttaccgat cttatcccta
     2701 ctacagagaa cattgcaaag gtgctgtggg atgtgataag cgaaaggctt gaatctcaca
     2761 gctgcagact gcacaggata agactgtatg agacaccgga gctttatgtg gattactgga
     2821 gggataaatg agttctgtaa atattcctga acactgctgc cttactaaaa tttattcctt
     2881 ctccgcaggg cacaggctct cgcttgacag gctcacacca gaggaaaatt caagagtatt
     2941 cggcaaatgc tccaacccaa gaggtcacgg ccatgactat tatctcgaag tcagggtaga
     3001 ggggcagatc aatccggata ccggcatgat agttcattta aatgaattgg attcctcagt
     3061 aaataatata atttccgatc tcgatcataa gaggctcgat attgaggtag actattttac
     3121 ttctaatcag tccacgggcg aaaacatagt caggtatttt tggggtaaac taaagagtaa
     3181 tgtagaggat tgtaatctcg tataccttaa gctctgggag accgagaaca actactttga
     3241 atactttgag gagaaagaca taaaatatgc caattgaaga tttgataaag gatatgcttg
     3301 ttgagatagg agaagatcct gagaggcagg ggctagcaga tactccatcg agagttgcaa
     3361 ggatgtacaa ggatgagctt acttccggct acaccaagtg ccctcataaa gttataaacg
     3421 acgcaatatt tgatgagaag tatgatgaga tggtggtggt aaaggacgtg gagttttaca
     3481 gcctgtgcga gcaccatatg cttccgtttt ttggcaaatg tcatgtggcc tatgtgcccg
     3541 acggaaagat catcgggctt agcaagattc caagactcat agaagtctat tcacgcaggc
     3601 ttcaggtgca ggagcggatg accatgcaga tagcagatat attgaatgag tccttaagtc
     3661 cccacggggt agcggtggtg gtggaagcgt atcacctttg catggcaatg cgcggtgtaa
     3721 agaaggcaag ggcaaacatg ctcacaagct ccatgctcgg ggtgtttaaa aaagacgagc
     3781 ggacaagggc tgagttcctg agtctaatcg ggcagagcaa tatcaaataa taaatgaaaa
     3841 aagtagtact cataacagga gcgggtaaag gcattggaaa ggagaccgcg cttacttttg
     3901 caagggcagg ttttataata gcggcctgcg gaaggacggc atcagacctg aaaactctaa
     3961 aaaggcagat tgaaaaagca ggttcagcgg ctgtaacgtc tgtgtgtgat attagtaaag
     4021 aatcccaggt taagcgtttt atagcaaaag ccgtaaagat caccggcagg attgatgccg
     4081 tagtaaacaa tgcggccgta gcctccattg gttccatata cgatacagac ctcaaagact
     4141 ggcagaacac tatcgataca aacctcacgg gcgcgttcct tataacaaaa cattcactag
     4201 aacatatgga aaagggcggg catattttca acataggctc aaacgcatcc aagattggat
     4261 ttcccaactg gagcgcatat tgcgcctcaa agttcgggct gcttggtttc agtaattcaa
     4321 taagggaaga actgcggtca aaaggtatta aggtatcggc catccttccg ggaccgacaa
     4381 agactccact ctgggggaag cttggtaaga aaaaatggga taaatcgaag atgatggagc
     4441 ctgcaggcgt tgccgagatg atcctgcatg tttataatca acctgccgac gtacagaccg
     4501 aagaagtcta cattattcca tcaagcggtg gtttataatc tgatctattt gtgttatata
     4561 ttatctagtg tatatcttga tcttttaaga ctggcataat ttttgcctta tgtataagca
     4621 tatatcaagc acatactaga gctgccaaat gcataacata tttacattag gtattagtta
     4681 taaggataca cctgttgagc ttagggaaaa gctttcattt agcggtaatg atacagagcg
     4741 tgtactgtcc gaaattctga cagacacagt gcttgaagag tgtatcatta tttccacatg
     4801 caaccgcatt gagttttacg ccattgccga ggatattgaa ttatgctctg atcagataaa
     4861 gcagtttgtg tgcaattaca acaaaataag ctcagataag cttaactctc atttctatct
     4921 gtacacgggt gcacaagccg tgagacatct gtacagggtg tcgtcgagtc ttgactcgat
     4981 ggtcgtagga gagcctcaga ttctggggca ggttaaagat gcgtataaga ctgctacaaa
     5041 ggccgacagt acgggtctta tacttaataa actttttcac agcgcatttt ttacggccaa
     5101 aaaagtacgt tcggaaacgg gtattggctc tcaggcggta tctataagct acgccgctgt
     5161 ggagcttgca aagaggatat tcgatgattt ctctgagaga acagccgtgc ttataggttc
     5221 gggagagatg ggagaactcg cggcaaaaaa tcttataaac gtgggaatta acgagcttat
     5281 aatagcaagc aggaatataa ataatgcaag ggtgcttgcc cagaatctgg gaggcaaagc
     5341 tgtgggtatg gaagaggttt attaccacat aaaccatgcc gatattatca tcaccgccac
     5401 agattccatc gattacataa ttaagcctca ccaccttaat gaggcgctcg gcgtaagaaa
     5461 aaacgagccg atattcctta tagacattgc cgtgccaaga gacattgacc caaggattca
     5521 tgattttgag aatgtgtacc tttatgacat agatgacctt gccggcgtgc ttgaagaaaa
     5581 catagagagt agaaaaaaaa gcgctaaaag agcagaagag ataataaacg aaagggttga
     5641 tcgattttct cactggatag agcagctgaa ggtctttccg gcaatagtcg agcttaagaa
     5701 caggtttgag cttataaaga accttgagac ggataaggcg ctcaggaagc ttgagaattc
     5761 tcaaggtgat gaaaaagaaa taatcgagaa gatggcttct tctctaatct ccaaacttct
     5821 acacagccct ctatcaaacc tcaaaaaaga gtcttccgga tattcgggtc tgatatatgt
     5881 agatgctatt acaaaactgt ttgatctcca aactaaacta acactggtgg atgatgagtc
     5941 tgatgaagag ccttctaagg attggaacga gaggcagtaa gcttgcgcta tggcaggcaa
     6001 acttcgtaaa ggactccctg cagaccattt tcccaaagct tgttgtttat ctcaaaatta
     6061 taagaacctc cggggacagt tttaccgaca gaccgcttgc tgaaattgga ggcaaggggc
     6121 tatttgtaaa agagatagag gaagcgcttt tagccgacaa ggtagatata gcggttcaca
     6181 gtatgaagga tgtgccttat gagcttccgc cgggactgga aatctctact ttcttaaaac
     6241 gtgaagaccc aagggatgca ttgctatcta atggacatgt gaaatttgca gacctgcccc
     6301 ctggtgcaac tgtaggcact ggaagcctcc gccggcaggt gcagattcaa agactaagag
     6361 acgatctgga cattaaacca ctcaggggaa atgtggatac gagaataaga aaacttatag
     6421 atggacagta tgatgctata gtgcttgctt cagcggggct aaacaggctt gagcttggaa
     6481 gtgaaattac agagcatttt tcagtgtctg atctcatccc atcacccggg caggggataa
     6541 ttggcataga gagcaggata gatgacaaag atacacggga aattatctcg aggctaaaag
     6601 atgctgacgc tacatgtgcc atcggggcag agagaaattt tgtaaaacaa atgggaggtg
     6661 actgcagcct accgcttggg tgttactgtg agattagcaa tgacactgaa attgaaattt
     6721 ccgggtttgt agcatcagtt gacggcaagg atttggttaa agatacagtc tcaggggcaa
     6781 ctcatgaaaa tataaagctc gctgaacagc ttgcaaaaaa aataatctca aatggcggca
     6841 aagagatcat tgagagtttt taaacttgat cgtgatagtt cctacgatgg gccagtgggg
     6901 aatgcttttg gctgcgatag ttcttggttt ttttgcaata ctaagactaa gaaagataga
     6961 cgaatctgaa tttaaataat caaacaacta aaacttagga tatgtaatca agggccggga
     7021 ataaaccccc ggcctttttt gtttgctcct accaaaatat aacgatactg attaattcta
     7081 tctattgatt ttacttttta gctacaactc ttaatataat attattatgt tctctgacat
     7141 gttggcaagc aggattgagg acgtcgagtt ttgtattctc gatgtcgaga ccacgggtct
     7201 aaaccccatt gcaggggata agatctgtga gattgccatc gttaaatcaa gagactccaa
     7261 aactctaggc acatacgaaa cgcttgtaaa tcccgggata aaaataccca tttctgctac
     7321 aggtatacat gggattcatg atgaagacgt atccgaagcg ccgtattttc tggatattgc
     7381 agaggacata cagaggtttc ttgcaggctc ggtaatcgtg gctcataacg cacggtttga
     7441 cctagggttt ctggacgcgg agcttaattt tactacagat atttcagtga aaaaccccat
     7501 agttgatact ctggggatag ccaggaaatt ttacactttt cccaccaata acctgggtgt
     7561 gatcgcaaag acactgggta ttcccactgc aggctaccac agggctatgg ccgatgtgga
     7621 gataacaaaa gacgtgctgg agtattttat aaaagacctc cgcaggcgtg gtataaggct
     7681 caatacactt aaggatcttg taagagtaca gggcgggggg atagagattg ataggccgcg
     7741 tgacagcata cttcccaagg ttattgagga tgcgctcata aacaaggcaa agcttcagat
     7801 aaagtatctc tctcctttta gtgactcggt aaaagacagg gtgattgagc ctctggatgt
     7861 gcttgagaga aggactaata catatttgat agcttattgt cacttaagga agcagaagtg
     7921 caatttcagg cttgaccgga tacttgagat aagcactgta agctcataaa ctgatttatt
     7981 cgccatttac ctattgactt tgttgctaac tcttttacta ttaccagttc taaatcggac
     8041 gcggggtgga gcagtatggc agctcgtcgg gctcataacc cgaaggtcgg aggttcaaat
     8101 cctccccccg caaccaatta tttcaggtag ttactacact ttctgaattc ttattctgga
     8161 ttaatttccc cctgatttat cttaaatctc aggttttata ccccaatata tgtataaatt
     8221 ttgctaattt tgcatatcta gtgttttttt gatgcttctt tgcaatttca tttacaaaaa
     8281 aattgattta ttactggcac aagtattgct ttatatttaa tgtattaatt gttataaata
     8341 tagatgacag ttaagaactg acgctaaggg tgaggcatta tttagttttt gaaacttttt
     8401 ataaactaat aattaaatgt gcatataatt aaaatagaaa tatgtcacta agaaaaaaga
     8461 ctgtattaat agtaggtatt gcttcggtaa ccattgttct tgtcttgtat tctataatat
     8521 cccgaatatt gcttgatagt tatagagaac ttgaaaatca ggatacgaaa aaaagtgtaa
     8581 atcaggttct aagcaagatt tcagatgaac tggacaattt gagttcaact gcagacaact
     8641 ggtctgccag gaatgaaaca tataatttta tgttgggtga cagcaggaaa tacgctgaag
     8701 aaaatttact taataactct cttaaaaacc agaaactcaa ttttataatt tttgtagata
     8761 aaaaaggaga tatatttcat cacaaggggg tagattacaa aagtggaaaa gatattgcac
     8821 ttcccggtga tcttgccgat attataagtt cgcaaagtac tgttgtaaaa catgcttatc
     8881 cttctagcag cacaagtggt tttattcaaa ccaaagattc tactctaatg tttgcatcca
     8941 gaccaataat caataatgac ttgactctgc caatagccgg caccctaatt gtagcaaggt
     9001 tttttgattc atatgaatta aagtatatat cggaatcaac tctgcagaca gttcggttta
     9061 agggttataa tgacagacat gaagcagccg attgtgagca tgcagctgaa ataatctttc
     9121 acactaataa aatctatgta aatgaattag atgaaacgct tatagccgga tatgcgctca
     9181 taaaagatat atttgataag ccggcattaa ttgtaagggt agataagcaa agaagtatat
     9241 ttatgcaggg caaaaagacc attaattatt ttctgttagc tttaatactg ataggaattg
     9301 cattttgtat tttaatttac ttctttttgg aaaatttcgt catttcaaaa ctcttaaaat
     9361 ttagtgatag tgtaaacaac ataacatcaa atcaggattt ctcatccagg ataagctttt
     9421 caggaaagga tgaactttca aatctgggga caaatattaa tgaaatgctc cagagtctcg
     9481 aagataagca aaaaattatt aaaaccacaa atgatgaatt ggaaagtagg gtaaaagaac
     9541 gtacgcaaga actacttaca gctaacaccg ctctggagaa agaaatcaaa aacagaatgg
     9601 aaaaagagga agaattaaaa gtaaatgtac aatctctttc caaaaaaaat aagtacgaga
     9661 tgataataag atcggttgca gaaagtgtta ataaaacaaa agatttacaa caaattatag
     9721 acaacgcgat taagtcaatt catgacaata tagaacatgc aaccactatt tctctttatt
     9781 tagtgactgg taatgttgcg caattaaaag gtgccattgg acatccacag tggtttatag
     9841 atagagtgtc aaatataccc taccccaaag ggtttacatg gaaaacaatt attgataata
     9901 catcgcttct ttgtaacgac actgatttgg atactgctat ggggcaggca ggaaaagatt
     9961 tgggaataat gagttacctt tcggtaccct taaggcatgg tgaagaatca gttggatgtc
    10021 ttaaagttga ctcatttcag aaaaactcgt tttcatcaga agatcaaaag ttgcttgaaa
    10081 ttgttgcaag acaaatagat gtggcaatcg ataactctaa agcagatttt gcgcttaaaa
    10141 ctaatattga acagttatcc agaaagaata aacatgaatc cattattcgt actgttactc
    10201 aaagtgtgca tcagtccat
//
LOCUS       DBNGSV010000096         3703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_432983, whole genome shotgun sequence.
ACCESSION   DBNGSV010000096 DBNGSV010000000
VERSION     DBNGSV010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_432983"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC159U_03470"
     CDS             complement(<1..227)
                     /locus_tag="AC159U_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336146.1"
                     /translation="MQNIKSIDRSDFHLKVVQEIGDLINRTSGLNNILEQVVNKIGDA
                     LHFDIVSVYLWDTESEKLILKATRGLNVDPN"
     gene            complement(293..706)
                     /locus_tag="AC159U_03475"
     CDS             complement(293..706)
                     /locus_tag="AC159U_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3336147.1"
                     /translation="MLERWFIMALAATVLFALGSFFGKIASANDIPFRVYFFEGMGTL
                     TVLFTVIFLKRNEIFSAFSPNYAALAMGLCWGIGTVLFIIALKDAKLSVTVPLTGLYP
                     AITIILALVFLGEKLGPREVAGVSLAVVSAMLLAR"
     gene            862..2259
                     /locus_tag="AC159U_03480"
     CDS             862..2259
                     /locus_tag="AC159U_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3336148.1"
                     /translation="MNETKESKKTFNAAIIGSGPAGFYAAEHLFKNKDYSFNVDIYDR
                     LPTPFGLVRSGVAPDHQKIKTVTRVFDKIAQNPGFRFFGNVEYGKDINLDDLKKHYHI
                     IIFATGAQTDRKMNIPGEDLKGSYTATEFVAWYNGHPDYKEREFDLSQEKVAIIGVGN
                     VAVDVARILCRTTEELEKTDIADYALEKLANSNVKEVYMIGRRGPAQAAFTNPELKEL
                     GNLEGADLILDKDEIEPDKITIKQMQEHEDKSVTRKLELLKEYSQAATGDKDKKLYIK
                     FLLSPVEITGNDDSTIKSLVLTENELYESEDGSVRCKSIDKNIELDVGLVFRSIGYHG
                     VPLAGVPFNESWGVISNEKGRIVDNGNKHINGLYATGWIKRGPTGIIGTNKTDSAETV
                     NFILEDINENKLNTPELTDTESIENLIMSRNDKIITYEKWLKVDDSEKLRGEKLGRPR
                     LKYTDIDEIIDIANK"
     gene            complement(2271..2774)
                     /locus_tag="AC159U_03485"
     CDS             complement(2271..2774)
                     /locus_tag="AC159U_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336149.1"
                     /translation="MNRKYIITLCLLIVLIPVHKTQSSPLLSLKGISVIAVRVDKLPD
                     YMNEDGIKELEIIKNIEKQLKEKKIRIVDYQDWEKTLGAAYINIKIVPSKSYIGDQYV
                     IYTQLEVIQPVTLIRPQIEHNRLITANTWSAGKLVNCSTESVDQCLGSSIYNLVELFA
                     AELRKMN"
     gene            3183..>3703
                     /locus_tag="AC159U_03490"
     CDS             3183..>3703
                     /locus_tag="AC159U_03490"
                     /inference="COORDINATES: protein motif:HMM:NF047111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3336150.1"
                     /translation="MRYILALLTLMLMTFTSTIDVYAQNGPNAEFVEGVVLVKFKPGA
                     AKNKKKEAVDSVQGNTEKEYTIVPGLQKLSTNLDVEKAVEILSKNPNVEFAEPDFIVS
                     IVDGSQTIPNDTQFNLLWGMHDVGDSSPDNDINGPEAWSHYVGDPNLIMANIDTGVDI
                     NHEDLVGNIWFNPG"
BASE COUNT         1299 a          614 c          687 g         1103 t
ORIGIN      
        1 ttattaggat caacattaag accccttgta gcttttaaaa taagtttttc actctctgta
       61 tcccataggt agactgacac aatatcaaaa tgaagcgcat cacctatctt gtttacaacc
      121 tgttcgagga tgttgtttag acccgaagtt ctgttaataa ggtctccaat ttcctgtaca
      181 actttaagat ggaagtcaga tctgtctatg gattttatat tctgcatagc taattaagta
      241 tattctattt ttatgtatta aagaattgtt ttttaataga tgtaataata tgttacctgg
      301 caagaagcat ggctgacact acagctagac taaccccggc aacttctctt ggtcccagtt
      361 tttcacctaa aaacaccaga gcaagtatta tagtaatagc cggatacaaa cccgtaagcg
      421 gaacagtgac agagagcttt gcatccttta atgcaataat aaacagtaca gtacctattc
      481 cccagcaaag acccatggca agtgcagcat agtttgggga aaacgctgag aaaatttcat
      541 ttctttttaa aaatataacc gtaaagagaa ccgtaagagt acccataccc tcaaaaaaat
      601 agaccctgaa ggggatatcg tttgctgatg ctatctttcc aaaaaaactt ccaagagcaa
      661 aaagcacagt tgctgctagc gccataataa accatctttc taacattgtt aatactcctc
      721 tataactgat attttcaagc accttacata tagatagtaa cagattgtac tattaattaa
      781 tttgagtata ttaacagccc ttaaatgtct aaatagtaac aatatcttat agatctagaa
      841 atatataatc ttatatatga aatgaacgaa acaaaagaat ctaaaaaaac ttttaatgcg
      901 gcaatcatag gctcaggacc tgcgggattt tatgctgccg aacatctgtt caaaaataaa
      961 gattattctt ttaatgtgga catctatgac cgactgccta ctccttttgg ccttgtaagg
     1021 tcaggcgtag ccccggatca tcaaaaaata aagactgtaa cgagggtatt tgataaaatt
     1081 gcccaaaacc cgggattcag gttttttgga aatgtggagt atggaaaaga tattaacctg
     1141 gatgatctta aaaaacatta tcatataatc atttttgcaa ccggtgctca gacagacaga
     1201 aaaatgaaca tcccgggaga agatcttaag ggttcttata cggcaacaga atttgtagcg
     1261 tggtataacg gacatcctga ttataaggaa agggaattcg accttagtca ggagaaagta
     1321 gcaataatag gcgtgggaaa tgtagcagtg gacgtagcaa gaatactatg caggacaaca
     1381 gaggagcttg aaaaaactga tatagccgac tatgcgcttg aaaaactggc aaatagcaat
     1441 gtaaaagaag tctatatgat tggaagaaga ggtcctgctc aggcagcatt tacaaaccct
     1501 gaattaaagg agcttggcaa tcttgaaggg gcggatttga ttctggataa agatgagata
     1561 gagcctgaca agataacaat caaacagatg caagaacatg aagataagtc agtaaccaga
     1621 aaactagaac tgttaaaaga gtattcacag gccgctacgg gtgacaagga caaaaaactg
     1681 tatattaaat ttttactctc accagtagaa attacaggca atgatgacag tactatcaaa
     1741 tccctcgtac ttaccgaaaa tgaactttat gaatcagaag atggatcggt gaggtgtaag
     1801 tcaattgata aaaatattga gctagatgta ggtctggtat ttcgctctat cggatatcat
     1861 ggagtacctc ttgccggagt tccgtttaat gagagttggg gtgttatatc aaatgaaaaa
     1921 ggcaggatag tagataatgg caacaaacat attaatgggt tgtatgccac aggatggatc
     1981 aagcgcggac ctaccgggat tattggtaca aacaagactg attctgccga gactgtaaac
     2041 tttatactcg aagacataaa cgaaaacaaa ttgaatacac cggaattaac tgatactgag
     2101 tctatagaaa atcttataat gagtagaaac gataagataa ttacctatga aaagtggctc
     2161 aaggtagatg attctgaaaa actaagaggt gaaaaactgg gaagacccag acttaaatac
     2221 accgatatag atgaaataat agatattgcc aataaataat ctaaattgtc tcagttcatc
     2281 ttcctaagtt cagcagcaaa aagttccacc aggttgtaaa tactgctgcc aagacactga
     2341 tcgacagatt ctgtagagca gtttacaagt tttccagctg accatgtgtt tgctgtaata
     2401 agtctgttat gttcaatctg tggtcttatg agtgtaacag gctgtattac ttctagttgt
     2461 gtatatatta catactgatc accaatataa gacttacttg gtacaatctt aatatttata
     2521 taggcagcac caagagtttt ttcccagtct tgataatcga ctatccttat ttttttctct
     2581 tttaactgtt tttcaatatt cttaattatt tctagttcct ttattccatc ctcgttcata
     2641 taatcgggta gtttatcaac cctcactgca attacggata taccttttag tgaaagcagt
     2701 ggagaggatt gtgttttatg aacgggaatt aacactatta ataagcacag agtaataata
     2761 tattttctat tcatttttta atttataaat attttaggga atataattct tataaactct
     2821 aacattaggt aatttgttcc acaatcttat aaaatacttt ttatcaatat tcttataaat
     2881 attataataa tattatacaa aatacacgat tcctatgata ttatgttgat ataagagata
     2941 tagttctatt ggcataataa ttgcttaata ctaaagttag gaataaacac ttatgacaat
     3001 agttgtaaca aaatccagca atttggtaac aattaatgtc agtgattact gattttgata
     3061 ataatcatac tgtaactatc tgtatttatt gaattataaa tttaggactt gaaaaggaac
     3121 aaaaattgca tcatacttta atactcttta gtacctgcgg gtaaaataaa ggaggattcg
     3181 agatgagata tattttagca ttgttaacat taatgctcat gacatttaca tcgactatcg
     3241 atgtttatgc tcaaaacggc ccaaatgccg aatttgtgga aggagttgtt cttgtaaagt
     3301 ttaaaccagg ggctgccaag aacaagaaaa aagaggccgt ggatagtgtt cagggtaata
     3361 ccgaaaaaga atatactatt gtccccggac ttcagaaatt atccacaaac ctggatgtgg
     3421 aaaaagcagt agaaatacta agcaaaaatc caaatgtaga atttgcagag cctgatttta
     3481 ttgtaagcat cgttgatgga agtcagacaa tacctaatga tactcaattc aatctacttt
     3541 ggggtatgca tgacgttggt gacagttcac ctgacaatga tataaacggc cctgaggcat
     3601 ggtcacatta cgtaggtgat cctaatctta taatggcaaa tatagacacc ggtgtagata
     3661 ttaatcacga agatcttgtt ggtaatattt ggttcaaccc ggg
//
LOCUS       DBNGSV010000097         1439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_779842, whole genome shotgun sequence.
ACCESSION   DBNGSV010000097 DBNGSV010000000
VERSION     DBNGSV010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_779842"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..998
                     /locus_tag="AC159U_03495"
     CDS             195..998
                     /locus_tag="AC159U_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3336151.1"
                     /translation="MEELKPKLILTKSGKSGFNIGRYIFFIIIFSIGFYFGYSYKSLD
                     SDIISQSKDQLTKIKDKKFTNLEFKDSAKKQPDNKITVSKNKTVKVKAVAFNKTDGLI
                     DKKSDNDIASLQIKKNVADMGSVEKVEKLPKTDTAILANNVTEKTDNKAKNSNTVKKE
                     QISQSEHKLVINDSQHVDTSYTLQIAAFETERKSSEVVQELSNKGYNVYSISVVNAHG
                     DRWNLVRLGNFETLEEAREFSELLLSSEGINSIVKSIEKSKVTSSTKFY"
     gene            1230..>1439
                     /locus_tag="AC159U_03500"
     CDS             1230..>1439
                     /locus_tag="AC159U_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336152.1"
                     /translation="MEASANMADTKSDSDNDNSGSDVFSPKYDSDKDNLKQLGIAGSA
                     VAEMDEDEFEFEQQPTTKELQKIEEE"
BASE COUNT          566 a          196 c          242 g          435 t
ORIGIN      
        1 taacaataaa ttgaatttaa tgacttagaa aaataaatgt tgaaaaattc caaataattt
       61 ctatttaaat ggttatttga taataatatg gtttaattat atcagttaag ttattctttt
      121 tatggaaaat agttgcttaa acacatagtt tgggtgtata atagatcata gaaatcttgt
      181 tgtggggatt ttttatggaa gaattaaaac ctaaactcat acttactaag tccggcaagt
      241 cgggctttaa cattggaaga tatatatttt tcatcataat tttttcaata ggtttttatt
      301 ttggatatag ctataagagt ctggattcag atataatttc acaaagtaaa gatcaattaa
      361 caaaaattaa agataaaaaa ttcactaatc ttgaatttaa agattctgcc aagaagcagc
      421 ctgacaataa aattactgtc tcgaaaaata aaacagttaa agtaaaagca gtagcgttta
      481 ataaaactga tggtttaata gataaaaaat ctgataatga tatagcctca ctgcaaatca
      541 aaaaaaatgt tgcagatatg ggttcagttg aaaaagttga gaaattaccc aagacagaca
      601 ctgcaatatt agcaaacaat gttactgaga aaacggacaa taaagcaaaa aatagcaata
      661 cagtaaaaaa agagcaaata tctcaatcgg aacacaagct tgttattaat gattcccagc
      721 atgttgatac ttcttataca ctacagattg cggcttttga aaccgagcgt aaaagtagtg
      781 aagttgtaca ggagctttct aataaaggct ataatgttta ttcaatatca gtagtaaatg
      841 cccatggaga ccggtggaat cttgtaagat tgggcaactt tgaaacatta gaagaagcaa
      901 gagaattctc tgaattactt ctaagctctg aaggtataaa ttcaattgtt aaatcaatag
      961 aaaaatccaa agttacatct tctactaagt tctactaatt aagattcaag ctctatccct
     1021 gacaattcaa ttagcctcaa tatgcattgt tagcaattgc gtaataatat gatgttaata
     1081 tcctctaaat aatctgcaaa atattcaatt atcctataaa taataagata taattctgtt
     1141 tatttggcat ttgcgaacaa tagtgttgac ttttagagca aagtgcctta ttatttcagg
     1201 ttatatttta aaaagttaag aggtaattca tggaagcaag tgcaaatatg gctgatacaa
     1261 aatctgattc tgataatgac aactcgggat ccgatgtatt cagtccaaaa tatgacagcg
     1321 acaaagacaa tttaaagcag ctgggcatag caggcagtgc ggtagccgag atggacgaag
     1381 atgaatttga gttcgaacaa caaccaacaa caaaagaact gcagaaaata gaagaggaa
//
LOCUS       DBNGSV010000098         9326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1125394, whole genome shotgun sequence.
ACCESSION   DBNGSV010000098 DBNGSV010000000
VERSION     DBNGSV010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1125394"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..776
                     /locus_tag="AC159U_03505"
                     /pseudo
     CDS             1..776
                     /locus_tag="AC159U_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707012.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(826..1950)
                     /locus_tag="AC159U_03510"
     CDS             complement(826..1950)
                     /locus_tag="AC159U_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336153.1"
                     /translation="MKGIKNIYNLGKKEFLSLRNDKVLIFLIIYGFTFVIYSSAQDDN
                     MELRNASIAIVDEDRSQLSSRIYDAFPEPFFLTPESLDINKIDSAMDSGKFTFIVDIP
                     DGFESDVISGRSPIIQVNVDATAMSQAGNGSGYISRILADEVTEYLKGYKYSAAQPAG
                     VVIRIKFNPNLTGSWFMSIMEMINVATMLAILFTGAAIIREREHGTIDHLLVMPLTPL
                     EIMTAKFWANGLVIVLAMLFSLTFVVTLALQVHIQGSVPLFIAGTALYLFSMTSIGIF
                     IATISRSMPQLGLLCILVIFPMLTLSGGYTPLDSMPKIIEIITTLSPTRHFVSYAQAV
                     LFRDAGLLIVWKEFVIVAVIGLAFFIGSLMRFRKTITMAQ"
     gene            complement(1952..4717)
                     /gene="rbbA"
                     /locus_tag="AC159U_03515"
     CDS             complement(1952..4717)
                     /gene="rbbA"
                     /locus_tag="AC159U_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HIR3336154.1"
                     /translation="MSIYKSHGPFVAELANVILKYDKITALQDINIKFPSGCMVGLIG
                     PDGVGKSSLLALVSGVRKIQSGRVEVLDGDMSSGTHRKTICPRIAYMPQGLGKNLYHT
                     LSVFENVDFFARLFGQSAVERKSRINELLEATGLARFADRPMGKLSGGMKQKLGLCCA
                     LIHDPDLLILDEPTTGVDPLARRQFWELIEDIRSRRESMSVIVATAYMEEAERFDWLV
                     AMDEGRILSTGTPAEIKSQTDSATLEESFIKLLPEQKQFGHKKLSIPPRLSKDEEIAI
                     ETKNLTKMFGDFTAVDRVSFRIQKGEIFGFVGSNGCGKTTTMKMLTGLLPATEGEAKV
                     FGRQLIGSDIETRRLVGYMSQSFSLYSELTVLQNLQLHARLYHLPEDKIPLRIEDLSN
                     RMGLKDVLYEQTQKLPLGIKQRLSLAVSVIHYPELLILDEPTSGVDPIARDKFWQLLI
                     DLSREKGVTIFISTHFMNEAERCDRVSLMHEGKVLATDTPQNLINSKDSNKLVDAFIS
                     YIEQADESTISRGKNNLNSNNILKERSLISSNQNIYKRLFNLNSLWAFTRRESIELIR
                     DPIRMFFALVGPIIMMIVLGYGISFDVENLPYAVLDRDQTPDSRKYIENFSGSRYFSE
                     HVHINDYNELENRLRTGELRVAIEIPPNFGKDLKRGAEPQVGVWLDGGFPFRAETARG
                     YILGLHDLYLSQVKESSQWKRYEELPFTIETRFRYNQDMKSAFAIIPGLIAMMLALIP
                     TILTAVGVVREKELGSITNLYATPATRLDFLLGKQLPYVAIGILNFFSLWILSSVLFG
                     VPVKGSSIAFIAGAILFVMSTTGFGLLISAFTKTQIAALFGAFLFTMINSMTFSGFLQ
                     PVSSLVGGGKIIALTFPTTYFMNISRGTVTKALGFGDLYINYIMICIFIILFMLLSLK
                     FLKNQEK"
     gene            complement(4714..5676)
                     /locus_tag="AC159U_03520"
     CDS             complement(4714..5676)
                     /locus_tag="AC159U_03520"
                     /inference="COORDINATES: protein motif:HMM:NF052711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3336155.1"
                     /translation="MISINEKKYSFVILLIVLISILALLWFYKFREDDSDHIVSGNGR
                     LEATEIDIATKFQGRVAEILFDEGDMVKAGEVVARIDTQSLEAQLREALAMVNKAEKE
                     RAYAEALLSQKESECDLKRKKLARYEKLFKDGIVSEDKLDEAKTEVKISAAACDAAKA
                     RIDNADASVKSAVSVTERLKSDIEDCILKAPRDSRVQYRLAEPGEVLPQGGKVLTTID
                     LDDIYMTIFLPEVEAGKVRIGAEARIVLDAFAEDAFGAKVTYIASKAQFTPKEVETKT
                     ERQKLSFRVKVNLTETDSLPAKPGMPGNAYILTDKSAGWPEHLQ"
     gene            complement(5705..7669)
                     /gene="ftsH"
                     /locus_tag="AC159U_03525"
     CDS             complement(5705..7669)
                     /gene="ftsH"
                     /locus_tag="AC159U_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3336156.1"
                     /translation="MTSENNNQRSDIKKSPQNILLIYLLLLMAFLIWNGIKYANKQQD
                     EIKIPYSEFIKQLKSDNVRKVVIEEDKIKGSFLKAILFSDLTIYKQDMEARSKYDEEF
                     KDTGKTFTGFQTTFPQAIGDPNLLVLLEQHNVMVDVISSTRNKLITTLLSWLPFLLLI
                     LFFLWMSNQAMGRRGNMFGFGKSKARLYQGDKKVVTFDDIAGADEAKAQLQQEVDFLS
                     NPKKYHDIGAKIPRGILLIGPPGTGKTLLAKAVAGEAGVPFLSISASEFVEMFVGVGA
                     SRVRDLFAQGKQAAPSIIFIDELDAVARRRGAGMGTVNDEREQTLNQLLVEMDGFDER
                     LEVIILAATNRPDVLDPALLRPGRFDRQVALGLPDREGRKGILKIHTRRLKLDPDVDF
                     SLLARSSTGMSGADLANLCNEAALVAARKDNKKVCMADFEQAFDKVLLGEKRKLYMDP
                     KEREVIAYHEAGHAVAAWFIPEADQVHKVTIIPHGQALGVTQQLPTEDHYNLSKAYLM
                     ARLAVLLAGRTAEEIAIGEITTGAENDLIEATQLARKMVTRWGMGKLGLRAFRADEEH
                     PFLGYELSRGKDYSESTAAIVDEEVKKLLDKSHETVHKILVSNKEKLDATVHLLLKDE
                     TIDSKELEKILGPRGLLKIVEKPKQNTNDI"
     gene            8081..>9326
                     /locus_tag="AC159U_03530"
     CDS             8081..>9326
                     /locus_tag="AC159U_03530"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR3336157.1"
                     /translation="MRYYKGLIKFLSDPATYGLKNKNVNVIQTHASYVFIAGNFVYKL
                     KKPVNFGFLDFSTLGKRKYYCEQEVSLNSRLSNSYLGVVPVMYDRGNYSFQKNGVVCD
                     YLVKMKRIPERYFVKNLLAKNKISKREIKRISDRLHKFYILQTPPDKLPKYGSPKHIK
                     NTLKQNRELGEKFVGKTITRTAFETIKYFNNLFLRVNKERLERRVSNGYIKDCHGDLH
                     LEHVIVRQNEIEIFDCIEFNNSFRFIDYVCDIAFLSMDLDYNARFDLSRYFISSILNK
                     MEDSKTLRLVDFYKCYRAYVRGKVGSIRAEDKNLPKDEKVKSVQNAKKYFQLSTRYAL
                     FGSDPVVIVIFGLIASGKSTFADRLSSELGCNVISSDVTRKNLAGIPLTQKAGEHLYD
                     GLYSKNMTNKTYDEMFKQAHEQL"
BASE COUNT         2766 a         1908 c         1717 g         2935 t
ORIGIN      
        1 tgatcttagc tgtcctaata aaaggttgtg gaactatatg gttccgtata tagaaaagta
       61 cgatgcggtt attctaactc taaaagaata tgaaaaaaaa cttaaaatcc cgcaagtttt
      121 ttttatgcct gcgatcgacc cttttagtat taaaaacaag aaattgagca acaaagagat
      181 tgatgaacga ctggagcatt atggtatacc aaccgacctt cctctggttg tgcaaatttc
      241 acgatttgac agatggaagg accccgatgg ggttattgaa gcttttaaaa tagcgagaaa
      301 agaagttgac tgcactcttg ttttactggg caacgtggca acagacgatc ccgaaggaca
      361 gcagatttat gaatctgtta taagttcaaa agaggaccgg ataatcattc tttcgcgtca
      421 ggatacagca ttggttaatg cccttcagag cagaggttgt tattcaaaaa tctttacgcg
      481 aaggctttgg cttgacggta accgaagcca tgtggaaagg aaccccggtt atcggaggga
      541 atgtgggagg tatccgatat caaataaaag atggtgaaaa tggctttttg gtctcttctg
      601 tgcagcaaac tgcagacaga atcgtacagt tactaaaaga taaggaactg agaaaacaaa
      661 tgggcagcaa agccagagaa agcgttaaaa ataatttttt aatgcccaga ctgctagaac
      721 aatacctcga tttatttaac tcttttgaaa tccggtttca gttaaaatcc ttatagttac
      781 aaagttaatc aattgattgt gttaatcttt acaagtaaat cattattact gtgccattgt
      841 aatggtcttt cgaaatctca ttaaagagcc aataaaaaaa gcaagtccga ttacagctac
      901 aatcacaaac tctttccata caattagcag tcccgcatca cgaaacaata cagcctgggc
      961 ataacttaca aagtgtcttg taggagacaa tgtggttatt atctcgataa ttttcggcat
     1021 actgtcaagt ggcgtatagc cgcccgaaag tgtaagcatc ggaaagatta ccagtataca
     1081 tagcaggcct aactgcggca tggagcgaga tatggttgca ataaatattc caatggaagt
     1141 cattgagaaa agatatagcg cggttccggc aataaacagc gggacagagc cctgtatatg
     1201 aacctgtaac gctagcgtaa caacaaatgt aagagagaag agcatggcca aaacaattac
     1261 aagaccattt gcccaaaact ttgcagtcat aatttcaagt ggtgtaagtg gcatgaccag
     1321 caggtgatct atagttccat gctctctttc tctaataatt gcagcgcctg tgaaaagtat
     1381 tgcaagcatg gtagccacat ttatcatttc cattatgctc ataaaccagc ttcctgtcag
     1441 gtttggatta aactttattc ttataaccac ccccgcgggt tgtgctgcgg agtatttgta
     1501 gccttttagg tactcggtta cttcatctgc aagtatccgg cttatgtatc ccgacccgtt
     1561 tcctgcctga ctcattgcag ttgcatctac attaacctga atgatggggc tgcgtcccga
     1621 aataacatct gactcaaaac catccggtat atcaactata aaagtgaatt tgcctgaatc
     1681 catagcagaa tctattttat tgatatcaag ggattcaggt gtaagaaaga acggttctgg
     1741 aaatgcatca tatatacggc ttgatagctg tgatctgtct tcatctacaa tggcaattga
     1801 agcattcctt agctccatat tatcgtcctg tgcagatgaa taaatcacga atgtgaaacc
     1861 gtaaattatg aggaatatca agaccttgtc attacgaagg cttaaaaatt cttttttccc
     1921 aagattgtag atattcttta ttcccttcaa tttatttttc ctgattctta agaaacttta
     1981 ggctcaatag cataaacaag ataataaaga tgcagatcat tatataatta atgtaaaggt
     2041 caccaaaacc aagcgctttg gtaacagtgc ccctgcttat gttcatgaaa tatgttgtag
     2101 gaaatgtcag tgcaataatt tttcctccac ctacaaggga tgaaacgggc tgaaggaagc
     2161 ctgaaaatgt cattgaattt atcatcgtaa aaagaaaagc tccgaatagt gctgcaatct
     2221 gagtctttgt gaaagccgaa atgagcaggc cgaatcctgt tgtagacatg acaaataaga
     2281 ttgctcctgc tataaaagca attgagcttc ccttaaccgg aaccccgaac aggacactgg
     2341 acagaatcca aagtgaaaaa aagtttaaaa ttcctatcgc tacgtatgga agctgttttc
     2401 caagtaaaaa atcaagtctt gttgctggtg ttgcatataa atttgtgatt gaaccaagtt
     2461 ctttttctct taccacacca acggctgtta gaattgtagg tataagagca agcatcatag
     2521 ctataagacc cggaataatc gcaaatgcgc tcttcatatc ctggttgtat ctgaatctgg
     2581 tttcaatagt aaaaggcagc tcctcatagc gtttccattg agaactctcc tttacttgag
     2641 aaaggtacag gtcgtgaaga cccagaatat agcctctggc agtctctgcc cgaaacggaa
     2701 aacctccgtc aagccaaaca ccaacctgcg gctcagcacc gcgcttaaga tccttgccaa
     2761 aattaggggg aatttctata gcaaccctta gctcacccgt tctcagacga ttttccagtt
     2821 cattgtaatc atttatatga acatgctcgg agaaatatcg cgagcctgaa aaattctcaa
     2881 tatactttct gctgtccgga gtctggtctc tatcaagtac tgcgtaagga aggttttcca
     2941 catcaaaaga aattccatat cccaatacta tcatcataat aattggaccg accagtgcaa
     3001 agaacattct aatgggatca cgtattaatt ctatagattc tcgcctggta aatgcccaaa
     3061 gactatttag attaaataac cgtttgtaaa tattttgatt agacgatata aggcttctct
     3121 cttttaaaat attatttgaa ttaagattat tttttcctct actaattgtg ctttcatcag
     3181 cctgttcaat ataagatata aatgcatcta caagtttatt actgtctttc gaatttatca
     3241 gattttgtgg tgtatcagtt gccagcacct tcccttcatg cattagcgat acacggtcac
     3301 agcgttcagc ctcattcata aaatgggttg aaataaaaat tgttaccccc ttttctctgg
     3361 atagatcgat aagaagctgc cagaatttat cccttgctat cgggtccact cccgatgtag
     3421 gctcatccag tataagaagc tctggataat gtattacaga cactgcaaga gaaagccgct
     3481 gttttatgcc taaaggtaat ttttgtgtct gctcgtagag gacatctttc aatcccatcc
     3541 tattcgataa gtcttctatc cgcaaaggaa tcttgtcttc aggcaaatga tacaatcttg
     3601 catgaagctg taagttttgc agtaccgtga gctctgaata caatgaaaag gattgagaca
     3661 tatatcctac aagcctgcgg gtttcgatat cacttccaat cagctgtcgt ccgaagacct
     3721 ttgcctcacc ctcagttgca ggcagaagcc ctgtaagcat tttcattgtg gttgtttttc
     3781 cgcatccgtt tgaacctaca aagccgaaga tttcaccctt ttgtatacga aaactaaccc
     3841 tatcgacagc tgtaaaatct ccaaacattt tagtgagatt tttggtttca atagcaattt
     3901 cctcgtcctt tgataatcga ggtggtattg ataatttttt atgtccaaac tgtttttgtt
     3961 ctgggagaag ttttatgaaa gactcttcga gtgtcgccga gtctgtctgt gatttgattt
     4021 cagcaggagt acctgtagat aagattcttc cctcgtccat tgctactaac cagtcaaacc
     4081 tttcggcctc ttccatgtaa gccgtagcca caataacact catgctttcc ctgcgtgatc
     4141 gtatatcttc tataagctcc caaaactgcc ttcttgcaag agggtctacg ccggtggtcg
     4201 gctcgtccaa aatcaacaag tcaggatcat gtataagtgc gcagcaaagc ccgagttttt
     4261 gcttcattcc acctgagagt ttacccattg ggcggtcagc aaaacgtgca agaccagtgg
     4321 cttcgagcag ttcatttatt ctggattttc tttccactgc agactgtcca aagagtcttg
     4381 cgaaaaaatc gacgttttca aacacagaca gagtatgata cagattcttc ccaagaccct
     4441 ggggcatgta ggcaatcctg ggacagattg ttttgcggtg tgttccgctt gacatatctc
     4501 catctaaaac ctcaaccctg cctgattgaa ttttgcgtac tccggaaaca agtgctaaaa
     4561 gactggactt tccaacacca tcgggcccta ttaaaccaac catacacccg gaaggaaatt
     4621 ttatatttat atcttgcaga gctgttattt tatcatattt gagaattaca tttgcaagct
     4681 ctgccacaaa ggggccatgg cttttataaa tactcattgt aagtgctcag gccagccggc
     4741 agatttatcc gtgaggatgt aggcattgcc tggcataccg ggttttgccg gaagactgtc
     4801 agtttctgtt aaatttacct tgactctgaa tgagagtttt tgcctctctg ttttggtttc
     4861 tacttctttt ggcgtgaact gtgcttttga agcgatatat gtaacttttg ccccaaatgc
     4921 atcttcagca aatgcatcca gaacaatacg tgcttctgca ccgattctta cttttccagc
     4981 ctcaacttcc ggtaaaaata tggtcatgta tatatcgtca agatcaattg tagtaagaac
     5041 ctttcctccc tgtggaagaa cctcgcctgg ttctgccagt ctgtactgaa cccttgaatc
     5101 acgaggggct tttaatatac agtcttcaat atcagatttg agtctctcgg ttaccgaaac
     5161 tgcggacttc acagaagcat ctgcattatc tatccgtgcc ttggcagcat cacatgcagc
     5221 agcagagatt ttgacttcgg ttttagcctc atctaactta tcttctgaaa ctattccatc
     5281 tttaaatagt ttttcataac gagctaattt cttccttttt aaatcacatt cactctcttt
     5341 ttgagataat agagcctcag cataggcacg ctccttttct gctttgttta ccattgccag
     5401 agcttctctt agctgtgctt ctaaagattg cgtatctatc cttgctacca cttctccggc
     5461 ttttaccata tctccctcat caaagagtat ttctgcgact ctgccctgaa acttagtcgc
     5521 tatatcgatt tctgttgctt caagccttcc gtttccagat acaatgtgat cactatcatc
     5581 ttctctaaat ttgtaaaacc ataacagggc taaaatggat atcagtacaa ttaacagtat
     5641 tacaaaagaa tatttctttt catttatgga aatcataagt atatccgcct tatatataca
     5701 ttctttatat atcgtttgtg ttctgtttgg gcttttctac tatcttgagt aatcctcgtg
     5761 gtcctaatat cttttcaagt tctttagaat caattgtttc atcttttagt aataaatgta
     5821 ctgtagcatc cagcttttct ttattactta ctaagatttt atgcactgtt tcgtgcgact
     5881 tatcaagcaa ttttttaact tcttcatcaa caatggcagc ggttgactca ctatagtcct
     5941 tgcctcttga aagctcatag cccaaaaacg gatgctcctc atctgctcta aacgctctca
     6001 aaccgagctt acccataccc catcgggtga ccattttacg tgcaagctgc gtagcctcaa
     6061 taaggtcatt ttctgccccc gtagtaattt ctcctattgc tatttcttca gctgttcgtc
     6121 ctgcaagcag cactgcgagt ctggccatca aataagcttt gctgaggtta taatgatctt
     6181 cagtgggtag ctgctgtgtt actccaagcg cctgtccgtg gggtattata gttactttat
     6241 gcacctggtc tgcctctggt ataaaccagg cagctacagc atgtcccgcc tcatgatatg
     6301 caattacttc cctctccttt ggatccatat acagcttgcg tttttcacca agcagaacct
     6361 tgtcaaaagc ctgttcaaaa tcggccatgc aaactttttt attatccttt ctcgctgcaa
     6421 caagtgccgc ttcattacag aggtttgcca ggtctgctcc gctcattccg gtactggaac
     6481 gagctagtaa tgaaaaatcc acgtcggggt caagcttgag tctgcgtgta tgaatcttta
     6541 atataccctt tcgaccctct ctatcgggta gtccgagtgc tacttgcctg tcaaatcgtc
     6601 cgggacgcag tagcgccgga tcaagcacat cgggtctgtt ggttgccgct agaataatga
     6661 cttcgagtct ttcgtcaaat ccatccattt ccacaaggag ctggttgagt gtctgttctc
     6721 gctcgtcatt cacagttccc atccctgcac ctcttcttct ggctactgca tcaagctcat
     6781 caataaatat tatagagggt gctgcctgct ttccctgagc aaacaggtct cttacccggc
     6841 ttgctccgac ccctacgaac atctctacaa attccgaggc gctgatgctc aaaaatggaa
     6901 caccggcttc acccgctact gcttttgcaa gtagagtttt tccggtaccc ggtggaccaa
     6961 ttagaagaat tcctcttggt atttttgccc ctatatcatg atatttcttg ggattactaa
     7021 gaaaatccac ctcctgctga agctgagcct ttgcctcatc cgctccggca atgtcatcaa
     7081 aagtgactac ctttttatct ccctggtaga gtctggcttt gctcttgcca aacccaaaca
     7141 tattacccct cctgcccata gcctgattgc tcatccatag gaaaaacaat attaataata
     7201 aaaaaggaag ccatgataga agtgttgtaa tcaacttatt tctcgtagac gatattacat
     7261 ctaccatcac gttatgttgt tcaagcagga ccagcagatt tggatctcct atggcttgtg
     7321 gaaatgttgt ctgaaaccct gtaaaagttt ttccagtgtc tttgaattct tcatcatatt
     7381 ttgacctggc ttccatatct tgtttataaa ttgtaaggtc tgaaaacaat atcgctttaa
     7441 gaaatgatcc tttgatttta tcttcttcta ttactacttt acgaacatta tcggatttga
     7501 gttgtttaat aaattctgaa taaggtattt taatttcatc ttgttgttta ttagcatatt
     7561 taattccgtt ccatataaga aaagccatta acaaaagtaa atatattaat aatatatttt
     7621 gtggtgattt tttgatatct gatctttgat tattattttc agaggtcatt ttatttgtat
     7681 ttattaatgt ttttaataag caatagtcta cataacttac tgatagttat catcaaataa
     7741 agtagtacta acgattttta gtgcaaacca tataccatgt ctaagcagtt caaattattg
     7801 gatttaatcc ctgtcaatat gcacagtgtc ttaaattagg acatacaata acattgttat
     7861 agataaagtt atttccaact taatatttat gatttaggta atgaaatata tctggttatt
     7921 tataattccc acaaaaacta tctctatatt agtaaaatag agtaataaat catagtttct
     7981 ttccaatgtg tacataaccg tctccattat aattatcttg gcaccgaagt tgcacttaga
     8041 tgtatatatt atttgttttt tatatagcct cgataaatga atgagatatt ataaagggtt
     8101 aattaaattc ttatccgatc ctgctacata tgggttaaag aataaaaatg taaatgttat
     8161 tcagacccat gcctcatatg tttttatagc tggaaatttt gtatacaaat taaaaaaacc
     8221 ggtaaatttt ggattcctgg atttctctac tcttggtaaa agaaaatatt actgcgaaca
     8281 ggaagtatct ttgaattcaa gacttagtaa ttcatatctt ggtgtagtac ccgttatgta
     8341 cgacagggga aattatagtt ttcaaaaaaa cggagtagtt tgcgactatc ttgtgaaaat
     8401 gaagaggatc cctgaaagat atttcgtgaa aaatttactt gccaagaata aaattagcaa
     8461 aagagaaatt aaaagaataa gtgatagact tcataaattt tacatcttac aaacaccacc
     8521 agacaaactg cctaaatatg gaagccccaa acacataaaa aatactctaa aacaaaatag
     8581 agaattggga gaaaagtttg ttggtaaaac tataactcga actgcattcg aaactattaa
     8641 atatttcaac aacttgttct taagagtaaa taaggaacga ctggaaagaa gggttagcaa
     8701 tggttatata aaagactgtc atggggattt gcaccttgag catgttattg ttaggcaaaa
     8761 tgaaattgag atatttgatt gtattgaatt taataatagt tttcgtttta tagactatgt
     8821 ttgtgatatc gcatttttat caatggacct tgattataat gcgcgctttg atctttcaag
     8881 atattttatt agctccattc tcaacaaaat ggaagatagt aaaacgctca gacttgttga
     8941 tttttataaa tgctacaggg catatgtgcg tggaaaggtc ggaagcataa gggcagagga
     9001 caaaaattta ccaaaagacg aaaaggttaa gtcagttcag aatgcaaaaa aatattttca
     9061 gctttctaca agatatgccc tttttggtag cgaccctgta gtaatagtaa tatttggatt
     9121 aattgcatcg ggtaaatcta catttgccga cagattatcg tcggagcttg gatgcaatgt
     9181 gatttcatcg gatgttacga gaaaaaatct ggccgggatt ccacttactc aaaaagcagg
     9241 ggagcatttg tatgatggat tatattctaa aaatatgacc aataaaacct atgatgaaat
     9301 gttcaaacag gcccatgaac aacttt
//
LOCUS       DBNGSV010000099         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1125646, whole genome shotgun sequence.
ACCESSION   DBNGSV010000099 DBNGSV010000000
VERSION     DBNGSV010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1125646"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..550)
                     /locus_tag="AC159U_03535"
     CDS             complement(59..550)
                     /locus_tag="AC159U_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336158.1"
                     /translation="MPIILDTQITDLEFKIPQPEVMDFTSNYFVGSRIPQDMIQSVFD
                     NTGIKTRQFISNREWFDSKHSFLDKNKLYTDQSVKVAYKLILKHQQEYNKKIDNIIFV
                     SSTGLSTPSIDALVINDLKLNNHITRTPIWGLGCVGGAVGDLPPYNSTRLQVRILGNK
                     PYE"
     gene            677..2146
                     /locus_tag="AC159U_03540"
     CDS             677..2146
                     /locus_tag="AC159U_03540"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3336159.1"
                     /translation="MEFNLIKGDVLKLKSNVIVVGKFEDESISKTVKKFDDALKGAIT
                     RLSEEENFKGGLGKLLNINTVGKTGSDRVLVVGLGNKSKFVPSDLKKVGIAVSKRVKS
                     FASKVIFALDIEANKLNAQNIIMGLILGTYDFNKYKSSNNSSNADTSYNFIWNKISQK
                     DFSSSVEKGTGIAEATCFTRDLVNEPPDTMTPSILADHAKKIAEDSGLECEIFNTVQI
                     KERKMGGLYAVSKGSLQDPRFIHLTYKPKKKARKKVAIVGKGITFDSGGLCIKPADYM
                     KTMKMDMGGSAVVLGVMKVISLVKPSLEVHALISASENMTGGGAYKPDDVVTAMNGKT
                     MEIVNTDAEGRVVLSDALSFAVELGVDEIVDLATLTGACLVALGTYTAGVMGNDQLVI
                     NRILKAANTVGEKIWQLPMDDELRKDIESDVADVKNVGSRYGGAITAGMFLEHFVDET
                     PWAHIDIAGPTFIEKGGEFYPKGATGFGVQTILQYLLER"
BASE COUNT          732 a          325 c          441 g          650 t
ORIGIN      
        1 ttaaatatct tattatttgc tctggcgtgt aacgcttcct gggcatttcc cttgcctcct
       61 attcataggg tttattacct aaaattctaa cttgtaatct ggtactatta taggggggaa
      121 ggtcacccac agcaccaccc acacaaccaa gaccccatat tggtgttctt gtaatatggt
      181 tatttagttt aagatcattt ataacaagcg catcgattga cggagtagaa agacccgtgc
      241 ttgatacaaa aattatatta tctatttttt tattatattc ttgctgatgt tttagtataa
      301 gtttataagc tacttttaca gattgatcgg tatacaattt gtttttgtct aaaaaactgt
      361 gtttgctatc aaaccattct ctattactaa taaactgtct agttttaatt cccgtattat
      421 caaaaacact ttgaatcata tcttgcggta ttcgagaacc cacaaagtaa ttggatgtaa
      481 aatccattac ctctggttga ggtattttaa attccagatc ggttatttga gtatcaagta
      541 tgattggcat tattattaat agatagcata aaagattaat ctcacattac taactaataa
      601 aattgattgg agataaatac ttatatggta gaattacact tcatattcat taactaatct
      661 taaggaggca agagcattgg aattcaatct aataaaaggc gatgtactca agctaaaatc
      721 aaatgtaatt gttgtaggta aatttgagga tgaatcaatt tcaaaaacag tcaagaagtt
      781 cgatgatgca ttaaagggcg caattacgag attatcagaa gaagaaaact ttaaaggcgg
      841 gcttggcaag cttttaaata taaatacagt aggaaaaaca gggtctgaca gagtgcttgt
      901 tgtaggcctt ggaaataaat caaagtttgt tcccagtgac cttaagaaag taggtattgc
      961 ggtttcaaag agggtaaaaa gttttgcctc caaagtaatt tttgctcttg atattgaagc
     1021 taataaatta aatgcacaga atattattat gggtcttatt cttggtactt atgattttaa
     1081 caagtacaaa tcatcaaata atagtagcaa tgctgatacg tcttataatt ttatctggaa
     1141 taaaatatct caaaaagatt ttagcagctc agtagaaaag ggaacaggta ttgcagaagc
     1201 tacctgcttt acaagagacc ttgtaaatga accccctgac acaatgaccc cgtctattct
     1261 tgcagatcac gcaaaaaaga ttgccgaaga cagcggactt gaatgtgaaa tattcaacac
     1321 tgttcaaata aaagagagaa aaatgggtgg actttatgct gtatcaaaag gcagcctgca
     1381 ggatccacgt tttatacatc taacttataa acctaaaaag aaagcacgaa agaaagtagc
     1441 tattgtaggt aaaggtatta cttttgacag tggtggtctg tgtattaagc ctgctgatta
     1501 tatgaagaca atgaaaatgg atatgggcgg atcagcagtt gtattaggtg ttatgaaagt
     1561 aatttcttta gtaaagccat cattagaagt acatgctctt atatcagcct cagagaatat
     1621 gacaggaggc ggagcttata aaccagatga tgttgtcaca gccatgaatg gaaaaaccat
     1681 ggaaatcgtg aacacagatg cagagggaag ggtagtgttg tcagatgcat tatcatttgc
     1741 agtagagctg ggagtggacg aaatagtaga ccttgctaca cttaccggtg catgccttgt
     1801 ggctcttgga acatacacgg ctggtgttat gggaaatgat caattggtta taaatagaat
     1861 ccttaaagca gcaaatacgg taggtgagaa aatatggcag ctcccaatgg atgatgagct
     1921 tagaaaggat attgaaagtg atgtagcaga tgtaaagaat gtcggaagca gatatggtgg
     1981 agcgattaca gccggaatgt tcttagaaca ttttgttgat gaaactccgt gggcacatat
     2041 tgatattgca ggtcctacat ttatagaaaa aggtggagag ttttatccaa aaggagctac
     2101 gggttttggc gtacagacaa tactgcagta tttattagaa agataaaa
//
LOCUS       DBNGSV010000100         4292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_849270, whole genome shotgun sequence.
ACCESSION   DBNGSV010000100 DBNGSV010000000
VERSION     DBNGSV010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_849270"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="AC159U_03545"
     CDS             complement(<1..594)
                     /locus_tag="AC159U_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3336160.1"
                     /translation="MLIINDSLNISWLGHGGLRINFKDKQIFIDPYKVSEGSADLILI
                     THSHLRHLSVEDISKLKKDNTIVCVPGDAGEVYDGGNIVVRPDVSFNLYGIPVYTTPA
                     YNLSAKPYHPKSKSWVGYILDLGGTKIYHAGDTDDIPEMKKLKVDVAFLPISGRSVMS
                     TRRAAEIANKFKPGIAVPIHWDGSPKSMQDAALFKKNY"
     gene            713..1057
                     /locus_tag="AC159U_03550"
     CDS             713..1057
                     /locus_tag="AC159U_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3336161.1"
                     /translation="MVNNTSESLNTVFGALSDPTRRQIIEMVSEEPVRITTVAAKFNM
                     SLPAVSKHIKKLERAGLINRDRDGRVHNIKLDSKPFHDASKWLDHYKKFWEQQFDSLD
                     DYLKKIDKRGKQ"
     gene            1054..1515
                     /locus_tag="AC159U_03555"
     CDS             1054..1515
                     /locus_tag="AC159U_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3336162.1"
                     /translation="MTESAQKQAEMPNWMLHLNREFAFPVEKVFSAWTEPEALKGWFC
                     NSGVAEFNPVVGGQFYVQMVTEKFGALELTGEYLEIIKNEKIKFTWKWLTEPLTQAGE
                     TVVTVEFLARDNSTEIKLTHEGFTTEEFKNNHTEGWDFAMENFEKVLKEQG"
     gene            1522..1698
                     /locus_tag="AC159U_03560"
     CDS             1522..1698
                     /locus_tag="AC159U_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336163.1"
                     /translation="MLKIYGSEISNNVNKVRFLANAMGLEYELVHVDLLHGEQREGGV
                     FKSKPGRQGARDGR"
     gene            1688..2164
                     /locus_tag="AC159U_03565"
     CDS             1688..2164
                     /locus_tag="AC159U_03565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3336164.1"
                     /translation="MDDDGFILFESSAISKYLADKHDSKYYPKELRRRALLDQWIDFC
                     NIHVHNSLVRVFFNKVIAPAQGFDVDERSSEFGEKMLASYFPVLDAELGKNEYLAGNK
                     MTIADINLLSILEPAEISGIDLSKYENLYKYTTGLRKQDFYTKCHSDYRDVLAKIM"
     gene            2219..2596
                     /locus_tag="AC159U_03570"
     CDS             2219..2596
                     /locus_tag="AC159U_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3336165.1"
                     /translation="MGFKDNVINWFEIPAKDIDRAVKFYNDIFNADLKVVDFNGTKMA
                     IFSEDYTKTGGAILQNEFSEPSKTGTTVYFSGGNDLSDVLSKVNNAGGQVVTEKTKIS
                     DEYGYFGIFIDSEGNKVGLHSMS"
     gene            2612..3295
                     /locus_tag="AC159U_03575"
     CDS             2612..3295
                     /locus_tag="AC159U_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR3336166.1"
                     /translation="MSEFEGFSKKSLKFFADLEKNNTKDWFSANRDIFDDEILFPAKA
                     FVTDMGERLTKIAPDIVADPRTDKSIFRIHRDTRFSKEKIPYKTHLGIYFWEGAAKKL
                     ENPGFYFQFDKDSMFLGVGMHIFPKDMIKDYRDAVVHKKKGVELRKAIESVTKKNKEY
                     ELGWVQYKKVPRGYDKDHPLADYLLYGGIGFHYKSKIPSEFNSEKLLDFTYKIFVDMS
                     PIHFWVKSI"
     gene            complement(3399..3836)
                     /locus_tag="AC159U_03580"
     CDS             complement(3399..3836)
                     /locus_tag="AC159U_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336167.1"
                     /translation="MKFYIIIAILSMVAVGQIGCDESKGQSSEADRFFLGLWEGIDPL
                     DGSTVRVSITNLDKDEKIEIAWTESLFTLCEGANGFITSTGTVDENGVINADEMLICF
                     DKEETAEGQASYTPVKDDGLIIVTPKSEPDFPPVILHRVSSSM"
BASE COUNT         1318 a          797 c          929 g         1248 t
ORIGIN      
        1 ataatttttc ttgaataatg ccgcatcctg catggatttt ggcgagccgt cccagtgaat
       61 gggaacagca atgccgggtt tgaatttatt ggctatttcg gcagctcttc ttgttgacat
      121 tactgacctg ccgctaatag gtaaaaacgc aacatcaact tttagctttt tcatctccgg
      181 aatatcatcc gtatcccctg cgtgatatat ttttgtgcct cccagatcga gaatataacc
      241 cacccaactc tttgattttg gatggtaggg ttttgccgat agattgtatg ccggggttgt
      301 ataaactgga ataccgtaga ggttaaaact cacatcgggt ctaaccacta tgttgccacc
      361 atcataaacc tctccggcat caccgggaac acaaacgatc gtgttgtcct ttttaagctt
      421 gcttatatcc tctaccgaaa ggtgccttaa gtgagagtgc gttataagaa tcaaatccgc
      481 acttccctct gacaccttat acggatcaat aaatatttgt ttgtctttaa agttgatcct
      541 caggccgcca tgcccaagcc atgagatatt gagtgaatcg ttaattatca gcattctctg
      601 gttgttgtga atatacgtaa acgaaatgca aatactgtgc cttcagattt atattaaata
      661 atatcccaaa ttcaggtttg acaaaagagg taaataattt atagttaacc atatggttaa
      721 caatacttct gagtctttga atactgtatt cggcgcgctt tctgatccca caagaaggca
      781 gattatagaa atggtttcgg aagagcctgt aaggattaca accgtggcgg ctaaattcaa
      841 tatgtccctt cctgcagttt caaagcacat aaagaaactt gagagagcgg ggcttattaa
      901 cagagaccga gacggcaggg tacacaatat aaaactggat tcgaaaccgt tccatgatgc
      961 atctaaatgg ctggaccatt ataagaagtt ctgggagcag cagttcgatt cactggatga
     1021 ttatctaaag aaaatagaca agaggggtaa gcaatgaccg agtcagcgca aaaacaggcc
     1081 gaaatgccaa actggatgct tcatctaaac cgtgaattcg catttcccgt agaaaaggta
     1141 ttttctgcct ggactgaacc tgaagcgctt aagggttggt tttgcaactc gggagtagcc
     1201 gagtttaatc ctgttgttgg aggccagttt tatgttcaaa tggttacaga gaagtttgga
     1261 gctctggagc ttaccggcga atatttggaa attattaaaa atgaaaaaat taagtttacg
     1321 tggaagtggc taaccgagcc tcttacccaa gcgggagaga cggttgtaac tgtggagttt
     1381 ttggcaagag ataactcgac cgaaataaag cttactcacg aaggctttac gactgaggaa
     1441 tttaagaaca accacaccga aggctgggat tttgcgatgg aaaatttcga aaaagtacta
     1501 aaagaacagg ggtagacaaa aatgcttaaa atatatggat cagagatttc aaacaatgtt
     1561 aacaaagtga ggtttcttgc aaatgctatg ggtctggaat atgagcttgt gcatgtagac
     1621 ctacttcacg gagagcaaag ggaaggcgga gtttttaagt ctaaacccgg taggcaaggt
     1681 gcccgtgatg gacgatgacg ggttcatcct ttttgagagc agcgcaatct ccaagtacct
     1741 tgcagataag cacgactcca agtattaccc aaaggaactg cgcagaaggg ctttgttaga
     1801 tcagtggata gacttttgca atatccatgt gcataactct cttgtaaggg tcttcttcaa
     1861 taaagtaatc gcccctgctc agggttttga cgtcgatgag cgctcgagtg aattcggaga
     1921 gaagatgctc gcaagctact ttccggtgct tgatgctgag cttggcaaaa atgagtatct
     1981 ggcaggaaat aaaatgacaa ttgccgatat caacctgctt tcaatactgg agcctgccga
     2041 gatatccggc attgatctca gcaaatatga aaatttatat aagtacacta caggactcag
     2101 gaagcaggat ttttatacaa agtgtcattc ggattacagg gatgtacttg caaagattat
     2161 gtgataatta ttttaaatct tagaaacaaa tacatgaata atataggagg aatttgctat
     2221 gggttttaaa gacaatgtaa ttaactggtt tgaaatacca gcaaaggata tagacagggc
     2281 tgttaagttc tataatgata ttttcaatgc agatttgaag gtagttgatt ttaacggaac
     2341 taagatggct atattctctg aagactatac caaaaccggt ggagcaattc tgcaaaatga
     2401 attcagcgag ccgtcaaaaa caggcacaac ggtttacttt agcggtggca acgatttgtc
     2461 agatgtgctt tcaaaagtaa acaatgcagg tgggcaggtt gtaacagaaa aaaccaagat
     2521 tagcgatgag tatggctact ttggaatatt cattgactct gagggcaaca aggtaggtct
     2581 tcacagtatg tcttaataag gggcagagcg tatgtctgaa tttgaagggt tttcaaaaaa
     2641 atcactaaag ttttttgccg atcttgaaaa gaacaacaca aaggactggt tcagcgcaaa
     2701 cagggatata tttgacgatg aaatattatt ccctgcaaag gcgtttgtga ctgatatggg
     2761 agaaagatta acaaaaatcg ctcccgatat tgtggccgat ccgcgtactg ataaatccat
     2821 ctttcggata catcgtgata caagattcag caaggaaaag attccgtaca agactcatct
     2881 ggggatttat ttctgggaag gggcagcaaa gaagcttgaa aacccggggt tctattttca
     2941 gtttgataaa gactccatgt ttttaggtgt cggtatgcac atctttccaa aggatatgat
     3001 taaagattat agagatgcag ttgttcataa aaagaaggga gtggagctgc gaaaggccat
     3061 agaaagtgtt acgaaaaaaa ataaggaata tgagcttggt tgggtgcagt ataaaaaagt
     3121 gcccaggggg tatgacaagg atcaccccct ggcagattac ctgctctacg gaggcatagg
     3181 ctttcactac aaatccaaaa tcccatccga atttaattca gaaaagcttc tggattttac
     3241 ctataagatt tttgtagaca tgtcaccaat tcatttttgg gtgaaaagta tttagcgcgt
     3301 agactcaata tgtcatttcg ggcttggcga aaaatctgtt tttaatacaa catacagtta
     3361 ctaatttctt gtaaacataa taaaggcttg tgagaaaact acatactgct gcttaccctg
     3421 tgaaggatca ccggtggaaa atcaggttct gacttaggcg ttactattat tagcccatca
     3481 tcttttacgg gtgtgtaact tgcctgtcct tctgcggttt cttctttgtc aaaacatatg
     3541 agcatttcgt ctgcattaat cacgccgttt tcgtcaactg tgcctgtaga agtaatgaag
     3601 ccgtttgctc cttcgcataa tgtaaatagc gattctgtcc atgcgatttc aattttttca
     3661 tccttatcca aattggtaat tgaaacacgc acggtagagc cgtccagagg gtcaatacct
     3721 tcccacagtc ccagaaaaaa cctgtctgct tcgctgctct gccctttgga ctcatcacac
     3781 ccaatctgcc ctaccgcaac catgctcaat attgctataa ttatataaaa cttcactgta
     3841 tttccctcca tcaaaaatta gtttttgata agttttttaa aaccgattca ggattttact
     3901 actatttatt cgcttaggca aaggagtgga aggaaatttt tgttatgtgt aaactgtttg
     3961 aaattgtcat attgtcatgt tttaaggtgt atagggtgaa aaggtaagaa tttgggaata
     4021 ttgggatttt cagactaatt tatgccttgc tattaattct ggtactttta aaaacgagat
     4081 cgtcacactt gtttaaggaa ggctaatatc accttaacgg taatatccgc tggaatgaca
     4141 gttgtggtta gtttttacca tgcccgcatt tcgaccaggc tcaatgcgtt tgattaaaag
     4201 cagatctctc ctaatttgaa ttcagacagt tgtttttaaa ctatcaaatt acagacttcg
     4261 atcatataca gccaaacttt atcttgacaa ta
//
LOCUS       DBNGSV010000101         8131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1680539, whole genome shotgun sequence.
ACCESSION   DBNGSV010000101 DBNGSV010000000
VERSION     DBNGSV010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1680539"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1216
                     /locus_tag="AC159U_03585"
     CDS             116..1216
                     /locus_tag="AC159U_03585"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR3336168.1"
                     /translation="MYKQIVSPILDHLDSETWHNFARESLHHCESFSPLLRLVELFNF
                     GARRLIDDRLKVNLSGIDFDNPVTVGAGWDKYGRAVKALWHLGFSGVEVGTVVFHPQP
                     GNPKPRQFMIAPGVGVNWLGFNSPGMDKIKENLNRYKNSGIPIGISIGKNKNLDDKEI
                     AWAHRAVAEELYDFASYFAVNVSSPNTPGLRKLQDKKPLGEIIAAVKDSMKGKKVKKP
                     VFIKIAPELTFHAIDEVIDVVEENKISGIIASNTTINTEIKAKYGTRWKESQGGLSGD
                     DPDYRKLVNDQIKHIYKQTKGKMEIIGVGGINSSETALEKIKAGAKLLQLVTAIRGEG
                     PSVAGKINTGLLNFMIKEGVKDVSELVGTDAD"
     gene            complement(1219..2400)
                     /gene="argJ"
                     /locus_tag="AC159U_03590"
     CDS             complement(1219..2400)
                     /gene="argJ"
                     /locus_tag="AC159U_03590"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3336169.1"
                     /translation="MIVKGFKASGIYSGIKRSKKKDLALIYSETPAQVSGVFTTNVVK
                     AAPVIIGQRKVKSGKCQAVVINSGIANVCTGKQGIKDANEITKAVAKELHIKDSLVIP
                     SSTGLIGAFLTSHLSKIKMSIPDIVNSLSEAGINDAAQAIKTTDAFIKMSSKKIKIDG
                     KQGTITAIGKGAGMISPNMATMLCFAITDIALEKTAQDRLLKSAVNDSFNKITVDGDT
                     STNDTVLLLTNGYAGNKPIKFNGRNYKKVEDALNGVLSDVAYKIVEDGEGATKVARIN
                     IKGTKTIKDADKLAHTIGTSTLVKTALYGQDPNWGRILGAAGRAGVKFNPDKIDLYFG
                     KHQVCKNGMAVMNEKKAAKYLKQKDIEITLDLNSGKKSSFILTSDLTVDYVKFNSDYT
                     T"
     gene            complement(2406..3104)
                     /gene="recO"
                     /locus_tag="AC159U_03595"
     CDS             complement(2406..3104)
                     /gene="recO"
                     /locus_tag="AC159U_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3336170.1"
                     /translation="MTEQITHAFVLKKTVYKEADYIISFYSSDFGKISCIARSAKKSS
                     KRFGGRLEPFLNLKIKFKPGIRHIKYLEDCSIIKSYADIMNDLDKYKWGSFILEYIEN
                     LSEAEEPNRDLYELLHSSIEHICEKNDIINMIPRFQYKALRCVGLTPELTACFSCGRD
                     VVDEGYLSIRQGGIACIECGKKLGAKVEGYKFIKKFDKNDKQTIMKNIALLTRFTKFH
                     TGKEFKSERFVMEN"
     gene            complement(3107..4459)
                     /gene="mgtE"
                     /locus_tag="AC159U_03600"
     CDS             complement(3107..4459)
                     /gene="mgtE"
                     /locus_tag="AC159U_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3336171.1"
                     /translation="MELQELKDQINKNIEDGNLHNIKSLLSEYHPSEIAEILDNIDPD
                     IHSNILSSLDNETASEVLLELDDEHRQDLLEDLDNERIADIVEEMDSDDAADVIADLA
                     DADVAPVLSKLDPEELEEVKTLLQYPEDTAGGIMQTELAEVTDDSTVRDAINWIRLIA
                     DEIDDLQDIYVIDDAEKLLGMVSPKKLLLSNPSTKLKNLMVEPEVTVTPLIDQEEVAE
                     IFEKYDIFALPVIDETGKLLGRITADDIIDVITEEASEDMYQLAGVQDQMHPIYTPTI
                     TRIKLRTPWLLLTLVGELLIALIIVYAFQPTLEKVAILAAFMPAIMATGGNVGIQTTT
                     IVIRSLGMGTITFSQIYKLVLSEVKVGLSIGVVCGLVAATAGIIIGKGEPQVLKLALA
                     VFIAMMSATMATSFIGAASPLLLQKFKFDPAAASGPFLTMFNDIFGSVVYLFIAMLIF
                     "
     gene            4602..5444
                     /locus_tag="AC159U_03605"
     CDS             4602..5444
                     /locus_tag="AC159U_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR3336172.1"
                     /translation="MGKLKNYGNFIKVEHSLFSLPLILCGSFLAVWGLPDLKLLCLII
                     IAAVGARTAALAINRILDAKIDVLNPRTKDRELPSGKINITEAIVITLSGLVIYLISA
                     YFICDLVLYLSPIPLVVFIIYPLMKRFTPFCHFGVGLGLALAPLAGWVAVTCSLSNLT
                     APMLLFLFTLFWVAGFDIIYATLDEKFDKEKGIYSIISVYGKDKGLLISAVLHILAFL
                     SIFVLYLLIFKSYISLLFLLITGVFLYLEHKKSENVDLAFFKINIVVGFSVFIFVLSG
                     IYLP"
     gene            5446..5997
                     /locus_tag="AC159U_03610"
     CDS             5446..5997
                     /locus_tag="AC159U_03610"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3336173.1"
                     /translation="MRTIIGFTGASGAAYGVDFLRRCPDEKYAIFSKWGKRVLHEELG
                     LKVEEVSNWATKTFSDSDLAAPFSSGSNHFDSLVIIPCSVSTLAKIANGIADTLITRV
                     AQVALKERRRLVIALRETPLSSIALENALKLSREGAVIMPISPPHYIDAENISELIEG
                     YVDKVLNVVGVETEGGWKYEELE"
     gene            6095..7879
                     /locus_tag="AC159U_03615"
     CDS             6095..7879
                     /locus_tag="AC159U_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HIR3336174.1"
                     /translation="MGRHNFHDLQQFIDHLEAIGDLKRITAEVDPILEITEIASRVIE
                     EGGPALIFENVKGAAFPMAINLFGTMERVNLALGREPREVGEELVYLFEKVNPPSFKS
                     FSTILPKAYDLLSMRTKNLRSGFSQQVEEQPNLSRLPIMQCWPEDGGRFITLGLVLTN
                     DPVTKRRNLGIYRMQVYDDKTTGMHWHPHKGGAAHYHEAAKLGQDLDVAVVLGGDPKM
                     IFTAIAPLPDGMDELAFASYLRGKPIPMVQGKSVNLKVPANAEFIIEGTVPQGVLREE
                     GPFGDHFGYYSMQADFPVFNVSEITRRLLPVFPATIVGKPPKEDIFLGIAAGEMFSPL
                     IKIIQPEVKRMWAYPEAGFHNLLVVSVDERYPKNGIKAMLALWGTGQLLLTKCMIMVS
                     SDVDPTDFSAVLTEIGENFYAKEDFLLIPWAPLDTLDFTSGKLNVGSKMGINAVRKPH
                     TSGRKKSPVPTSVPNPKEKHKEILDYKLLPGGILVLKITDQKEPKKVLEKMFKTPGYK
                     DVRIMILQSKDIDLNNQTELIWGMFTRFDPYLDVIFEHTELRGSAVIYDGRMGIDATY
                     KNSYPNVIEMSSNIKQKVSKRWKDYWKQ"
     gene            7855..>8131
                     /locus_tag="AC159U_03620"
     CDS             7855..>8131
                     /locus_tag="AC159U_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR3336175.1"
                     /translation="MEGLLETIEKLCSDKELLPILNKVEKEERLSFDEGLKILETDDL
                     NTVGMMADYVKRRREGDNVYFVVNRHVNPSNICAISCRFCAFGVTKKS"
BASE COUNT         2447 a         1620 c         1587 g         2477 t
ORIGIN      
        1 ggcttcatta cacttctata agcagaataa ttaacagtat ttaagtaggg tcttataatt
       61 ctttaatctt tttatctgat tttttttcat ttacagatat cctttctgta aattcatgta
      121 caaacaaatt gtaagtccaa tattagacca tctggattca gagacttggc acaattttgc
      181 ccgtgagtca cttcatcatt gtgaatcctt ttcaccacta ctaaggctag tagagctttt
      241 taattttgga gccaggcggc ttattgatga tagattaaaa gtaaacctca gtggaatcga
      301 ttttgataac cccgtaacag taggcgccgg ctgggataaa tatggaagag cagtaaaagc
      361 actgtggcac cttggatttt caggtgttga agtgggtaca gtggtttttc atccacaacc
      421 gggaaatcca aaaccaaggc agtttatgat tgcaccaggg gttggtgtaa actggcttgg
      481 gtttaatagc cccggaatgg ataaaattaa agaaaacctc aacagatata aaaactcggg
      541 tattccaatc ggtataagta ttggtaaaaa taaaaatctg gatgataagg aaattgcgtg
      601 ggcacacaga gctgtggccg aagagttata tgacttcgca tcttatttcg cagtaaacgt
      661 aagctctcca aataccccgg gcttgagaaa gcttcaggat aaaaaacctc ttggcgaaat
      721 tatcgccgcg gtaaaagact ccatgaaagg taagaaggtt aaaaaacctg tttttatcaa
      781 aattgccccc gagcttactt ttcatgccat agatgaggta atcgatgttg tagaagaaaa
      841 taaaatctct ggtattatcg catcaaatac aactataaat actgaaatta aagcaaaata
      901 tggcacaaga tggaaagaat cacagggcgg cctcagtgga gacgaccccg attacagaaa
      961 acttgtaaat gaccagataa agcatattta taaacagact aagggaaaga tggaaattat
     1021 tggggtaggc ggcataaata gttctgaaac tgctttggaa aaaatcaaag cgggagccaa
     1081 gcttcttcag ttagtaaccg ccatccgcgg cgaaggtcct tcagttgccg gaaaaattaa
     1141 caccgggctt ttgaacttca tgattaaaga gggtgtgaag gatgttagtg aattagttgg
     1201 aacagacgca gattaattct atgttgtata atccgaatta aatttcacat agtccactgt
     1261 gaggtcactc gtaagtataa aagaactctt ttttccgcta ttcagatcaa gagtgatttc
     1321 tatgtctttt tgttttaggt atttagcggc ctttttttca ttcattacag ccataccgtt
     1381 tttacaaacc tgatgcttcc caaagtaaag atcaatttta tccggattaa attttactcc
     1441 ggcccgtccc gctgccccta atatcctgcc ccagtttgga tcctggccat acagggcagt
     1501 cttaacaagc gtggaagtac caattgtatg ggctaactta tccgcatctt ttattgtttt
     1561 tgttccctta atatttattc gtgctacctt tgttgcccct tctccgtctt caactatctt
     1621 gtatgctaca tcgcttaaaa ccccattcaa tgcatcttca acctttttat agtttctgcc
     1681 gttaaatttt ataggtttat tccccgcata tccatttgtt aataagagca ctgtatcgtt
     1741 tgtagatgta tctccatcga ctgtaatctt gttaaacgaa tcattcactg cactttttaa
     1801 cagtctgtcc tgtgctgttt tttccaaagc aatatctgtt atggcaaagc aaagcatcgt
     1861 tgccatatta ggtgagatca tccctgcacc tttaccaatt gctgtaatag ttccctgctt
     1921 accatcaatt ttaattttct tagacgacat tttaataaaa gcatctgttg tctttatggc
     1981 ttgggctgcg tcatttatac cggcttctga gagtgaatta acaatatcgg gtatcgacat
     2041 ttttattttg cttaaatgtg aagtaagaaa cgctccaata agccccgtgg atgagggaat
     2101 aacgagtgaa tcttttatat gtaattcttt tgcaacagct ttcgtgattt catttgcgtc
     2161 ttttataccc tgcttaccgg tgcatacatt tgcaattcca ctgtttataa ctacagcctg
     2221 acacttgcct gacttaactt ttctctgccc aataatcaca ggagccgcct taaccacatt
     2281 tgtagtaaat acaccggata cctgtgccgg tgtctcagaa tatataagcg caagatcctt
     2341 tttcttactt cttttaattc cactgtaaat acccgatgcc ttaaaccctt ttacaatcat
     2401 ctgtactagt tctccattac aaatcgttct gacttgaatt ctttacccgt atgaaatttt
     2461 gtaaatcttg taagaagtgc aatatttttc attattgttt gtttatcatt tttatcaaat
     2521 ttttttataa atttatatcc ctcgaccttt gctcctaatt ttttcccaca ttctatacag
     2581 gcaatccctc cctgccttat gctcaaatat ccctcatcca ctacatccct gccacagcta
     2641 aaacaggcgg ttaattcagg ggttaaccct acacacctaa gcgctttata ttgaaacctt
     2701 ggtatcatgt taataatatc atttttttca caaatatgct caatgcttga atgcagtagt
     2761 tcatataaat ctctgttcgg ctcctctgct tcagacaaat tttctatata ctctagtata
     2821 aagctgcccc acttatactt atccaggtca ttcataatat ctgcataaga tttaatgatg
     2881 ctgcagtctt caagatactt aatatgcctg ataccaggct tgaacttaat cttaagattt
     2941 aaaaacggct cgagccttcc gccaaatctt tttgagcttt ttttggcact ccttgcaata
     3001 cagcttattt ttccgaaatc agatgaataa aaactgatga tataatcagc ttctttataa
     3061 actgtttttt ttagaacaaa agcatgtgtt atttgttctg tcatttttag aatataagca
     3121 ttgctatgaa tagataaaca accgagccga agatgtcatt gaacatggta agaaacggcc
     3181 ctgatgctgc agccggatcg aacttgaatt tttgcagtaa aagcgggcta gccgccccta
     3241 tgaatgacgt agccatagtt gcagacatca tggcaataaa cactgcaaga gcaagtttta
     3301 ggacctgtgg ttcacctttg cctatgatta taccggcggt agctgccaca agaccgcaga
     3361 ctacgccaat cgaaagtccc accttcactt cagagagaac aagcttgtaa atttgtgaaa
     3421 aagtaatagt gcccatacca aggcttctaa ttacaatggt tgtagtctgt atccctacat
     3481 ttccacccgt agccataatt gccggcataa atgctgcaag tatagcaact ttttccagtg
     3541 ttggctggaa ggcgtagaca atgataagag ctatgagaag ttcaccaaca agggttaaga
     3601 gaagccatgg ggtacgaagt ttaattcgtg taatagttgg tgtgtaaata ggatgcatct
     3661 ggtcctgcac accggcaagc tgatacatat cctctgaagc ctcttctgtt ataacatcta
     3721 tgatgtcgtc cgctgttatt cggcccagca gttttcccgt ttcatctata acaggcagag
     3781 caaaaatatc gtatttttca aatatttcgg caacctcttc ctggtcaatt aatggggtaa
     3841 cagtaacctc tggctccacc attaaatttt ttaattttgt actcggattt gatagtaata
     3901 gcttctttgg agaaaccatc cccagcaatt tttcagcatc atcaatcaca tatatatcct
     3961 gcaggtcatc aatctcatct gctatgagcc ttatccagtt tattgcatcc ctcacagtag
     4021 aatcatctgt aacctcggca agctcggtct gcataatacc accggcagtg tcttcagggt
     4081 actgaagtag tgtctttact tcttcaagtt cctcagggtc tagtttggac aacaccggtg
     4141 caacatcggc atcggcaaga tcggctataa catctgccgc atcgtctgag tccatttcct
     4201 caacaatgtc ggcaatcctt tcattatcca ggtcttcaag caggtcctgt ctgtgctcat
     4261 catcaagctc caataaaact tcagacgcag tttcattatc cagggagctt aagatatttg
     4321 aatgaatatc gggatcgata ttgtccagaa tttcggctat ttcagatgga tggtattctg
     4381 agagcaggct ctttatgtta tggagatttc catcttcaat gtttttattg atttgatcct
     4441 tcagttcctg cagttccata acaaatccct aatactaatt agattttggt attctaaaat
     4501 acaaacccta aatgtaaaga tttccactac ttatattaat taatttaaaa ataatgagtt
     4561 ataggtaaaa tcgaaataac aaaatccgaa ttgataaaat catgggaaaa ttaaaaaact
     4621 acggaaactt tataaaagtt gagcactcac ttttttcact acctcttatt ttatgcggga
     4681 gttttttagc ggtttggggt cttcctgacc taaagctctt atgtctgatt ataattgctg
     4741 ctgtaggagc tagaaccgca gcacttgcca taaataggat actcgatgca aaaattgatg
     4801 tactaaatcc tagaacaaag gatagggagc ttccctcagg aaagataaat attaccgaag
     4861 ctattgttat aacactatcg gggctggtta tttatctgat ttccgcttat ttcatttgcg
     4921 accttgtttt gtatctgagt cccatacctc ttgttgtgtt tattatctat ccccttatga
     4981 aaagattcac gccgttttgt catttcggcg tcgggcttgg gcttgccctt gctccccttg
     5041 caggctgggt agctgttacc tgttcgctca gtaatctgac tgctccaatg ttgttattcc
     5101 tgttcactct tttctgggta gccggctttg atatcatata tgcaacgctc gatgaaaagt
     5161 ttgataagga aaaaggcata tattccatca tttctgtata cggcaaagat aaaggtctgc
     5221 tcatctctgc cgtactgcat attcttgcat ttcttagtat ttttgttctt tacctgctaa
     5281 tttttaaatc atacatatca ctattatttc ttttaataac aggggttttt ctctatctcg
     5341 aacacaaaaa atcagaaaat gtagaccttg cattctttaa gataaatata gtagttggct
     5401 tttccgtatt tattttcgta ctaagtggga tatacttacc ctgatatgag aacgataata
     5461 ggatttacag gagcttcagg tgctgcatac ggggtcgatt ttttaagaag atgcccggac
     5521 gaaaaatatg cgatattcag caagtgggga aaacgcgtac ttcacgaaga gcttggctta
     5581 aaagtagaag aagtctccaa ctgggctaca aagactttta gtgactcaga ccttgcagcc
     5641 cctttttcat caggcagcaa ccatttcgat tcacttgtaa tcattccatg ttccgtttca
     5701 acacttgcta aaattgcaaa cggcatagca gatacactaa ttaccagagt tgctcaggtt
     5761 gcgctcaaag aaagaagaag gcttgtaatt gcacttaggg aaacccctct aagctcaata
     5821 gcactagaaa atgcgctaaa gttatctaga gaaggggcag taatcatgcc cataagcccg
     5881 ccgcattata tcgatgcaga gaacattagt gagcttatag aaggatatgt ggataaagta
     5941 ctaaatgttg tgggtgtaga gacagagggc gggtggaagt acgaagagct tgaataaatt
     6001 tcacctgaat tagtcgctaa actataaaaa tcagaatttt tctgttatac ctctttgctt
     6061 aaaaattaaa ttcagattca gtaataatat cattatgggt agacacaatt ttcatgactt
     6121 acagcagttt atagatcatc ttgaggcaat aggtgatctt aaaaggatta cggccgaagt
     6181 agacccgatt ttagaaataa cagagattgc ctcaagggtt attgaagaag gaggccctgc
     6241 acttatattt gaaaacgtaa aaggtgccgc atttccaatg gctattaacc tctttggaac
     6301 tatggagagg gtaaacctgg cgcttggaag ggaaccaaga gaggtaggag aagagcttgt
     6361 ttatctgttt gaaaaagtaa atccaccatc gtttaaatca ttttctacaa tactcccaaa
     6421 agcctatgat cttctttcta tgagaacaaa gaatttaaga agcggttttt cacagcaggt
     6481 tgaagagcaa ccaaatctat ccaggcttcc tattatgcag tgctggcctg aagacggcgg
     6541 aaggttcata acgcttggtc tagtactcac aaacgacccg gttacaaaaa gaagaaatct
     6601 gggcatatac cgcatgcagg tatatgatga taaaactaca ggtatgcact ggcatccaca
     6661 taaaggcggt gctgcgcact accatgaagc agcaaagcta gggcaggact tagatgtcgc
     6721 tgtagtactt ggcggtgatc caaagatgat atttactgca atcgccccgc ttcctgacgg
     6781 catggacgag cttgcctttg caagctacct tagaggaaaa ccaatcccta tggtacaggg
     6841 aaagagcgtt aatctaaaag tcccggcaaa tgctgaattt atcatagaag gcactgtacc
     6901 tcagggagtg cttagagaag aaggcccgtt tggcgaccat tttggctact attcgatgca
     6961 ggcagacttt cctgtattta atgtcagtga aatcacaaga aggctgctgc ctgtttttcc
     7021 ggcaactata gtgggcaaac ctccaaaaga agacatattc ctgggaatcg cggcagggga
     7081 gatgttttcc cctcttataa aaattattca gcctgaagtt aaacgcatgt gggcttatcc
     7141 tgaagccggg tttcataacc tgttagttgt ctctgtagat gagagatatc caaaaaacgg
     7201 aataaaagcc atgcttgcac tctggggcac gggacagctt cttttaacaa aatgcatgat
     7261 aatggtctct agtgatgtag acccgactga ctttagcgca gtacttactg agattggaga
     7321 aaatttctac gctaaagaag attttcttct cataccatgg gcaccgctgg atactttaga
     7381 ttttacgagc ggcaagctaa acgtcggaag caagatgggt ataaatgctg taagaaagcc
     7441 gcacactagc ggaagaaaaa agtctccagt tccaacatca gttcctaatc caaaagaaaa
     7501 gcataaagaa attctggatt acaagctttt gcctggtggt attcttgtat taaaaataac
     7561 ggatcagaaa gagccaaaga aagttctgga aaaaatgttt aaaactcccg ggtacaaaga
     7621 tgtcaggata atgatacttc agagcaagga tattgatcta aataaccaga ccgaacttat
     7681 ctggggaatg ttcactagat ttgatcccta tcttgatgta atattcgagc acactgagct
     7741 caggggttcg gcagtgatat atgacggtag aatgggaatt gatgcaacgt ataaaaattc
     7801 gtatcccaat gtaatagaga tgtcatcaaa tattaaacaa aaggtgagta aaagatggaa
     7861 ggattactgg aaacaataga aaaactttgc agcgataaag aactgttacc aatacttaat
     7921 aaggtagaaa aagaggagcg cctctcattt gatgaaggac tcaaaatttt agagaccgat
     7981 gatcttaaca ctgtgggtat gatggcagat tatgttaagc gcagaagaga gggagacaat
     8041 gtttactttg ttgttaaccg acatgtaaat ccatcaaaca tctgtgcaat ttcatgcagg
     8101 ttctgcgcat ttggagtcac aaaaaaatca g
//
LOCUS       DBNGSV010000102         2229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_849402, whole genome shotgun sequence.
ACCESSION   DBNGSV010000102 DBNGSV010000000
VERSION     DBNGSV010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_849402"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="AC159U_03625"
     CDS             complement(<1..1384)
                     /locus_tag="AC159U_03625"
                     /inference="COORDINATES: protein motif:HMM:NF020811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIR3336176.1"
                     /translation="MIDFYEKHGFKLFPCALSKAPAVPKKTDWRDPINHLTIDQALQR
                     MDSGHLVGAWLPKDFVIIDIDVNHTDGQGNIKSDGMKEFLKIADESYIKNTLVVKTGS
                     GGYHLYYKNAGDIPQGTLTTSVDVKTHSGYVIAAGSPGYSIYNINTIEDFPALFKEIL
                     NKPKNSKTNIDVKDPLSPSLLKRVLNKLDHKSFNTNDKWLELMFSCIAIAGNSEEIID
                     ILEAWSKEDINYSSDTSMRARIESLDPSGGITPATFIFILRREGISEHMIHKVRKEIG
                     AFLNLSTNIKDDYVIHFKVPFDRVADFNAVARAFFYAKDQANASRLFIELVRDNLIYS
                     AGEHQFYYFQSNRWEPIKGILRLIYAVMLRAADEYYVQYAANDGKDGDEAFGAIVNTI
                     GTGAWRQKIFTEISQADEICVQSPPWDSPKISETLTLRDGVVDFSGEEILYREGLQEE
                     YRKSYIDISVD"
     gene            complement(1809..2000)
                     /locus_tag="AC159U_03630"
     CDS             complement(1809..2000)
                     /locus_tag="AC159U_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336177.1"
                     /translation="MKVTFEELLKGQSKNNLIDMLNESLCTIEDEKKYGNSPLVLEHF
                     TVRVEKIQKELTRRLCMEK"
     gene            complement(1997..2158)
                     /locus_tag="AC159U_03635"
     CDS             complement(1997..2158)
                     /locus_tag="AC159U_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336178.1"
                     /translation="MTKLTIEVKRGERSKFYTLQGLPARKMIKKMRAGDLTMFNADRD
                     IFIKEIVLV"
BASE COUNT          639 a          455 c          348 g          787 t
ORIGIN      
        1 catctactga tatatctata taagactttc tatattcctc ctgtaaccct tccctgtata
       61 aaatctcctc acctgagaaa tcaactacac catctcttaa tgtcagtgtt tcggagattt
      121 taggcgaatc ccacggtggc gattgtacac agatctcatc agcctgggag atctcagtaa
      181 atatcttttg tcgccatgct ccggtcccaa tagtgtttac tattgctccg aatgcctcgt
      241 caccatcttt accatcattc gctgcatact ggacataata ttcatctgct gcccggagca
      301 ttaccgcata tatcaatctc agtattcctt tgattggctc ccaccgatta gattggaaat
      361 aataaaattg atgttccccg gctgaataaa ttaaattatc tctcacgagt tcaataaata
      421 accgtgaagc atttgcctga tctttagcat aaaagaatgc cctggctact gcattaaaat
      481 ctgcaaccct atcaaatgga accttaaaat gtattacata atcatccttt atattagttg
      541 ataaattaag aaaagctcca atttcttttc tcaccttgtg aatcatatgt tcacttattc
      601 cttcccttct aaggataaaa ataaacgttg ccggtgttat tccaccactt gggtccaatg
      661 attcaatcct ggctctcata ctggtatctg aggagtaatt gatatcctct ttactccatg
      721 cttctaagat atcaattatc tcttcactat tcccggcaat agcaatgcat gagaacatca
      781 attcaagcca cttatcattg gtattaaaac tcttgtggtc cagtttatta agtactcttt
      841 ttaatagtga tggtgataat ggatctttta catctatatt ggttttgctg ttctttggtt
      901 tgtttaggat ctctttaaat aaagcaggaa aatcctcaat tgtatttatg ttatatatac
      961 tatagccggg gctgcctgct gcaattacat aaccggaatg tgtctttaca tcaactgagg
     1021 ttgtaagtgt gccctgtggt atatctccgg cattcttata gtataaatga tagccacctg
     1081 aaccggtctt tactaccagt gtattcttta tgtatgactc atcggctatc ttaagaaact
     1141 ctttcatacc atctgattta atgtttccct gaccatcggt gtggttcaca tcaatgtcaa
     1201 ttattacaaa atctttaggt agccaggctc caactaaatg acctgaatcc attctttgta
     1261 atgcctgatc aatggtcaaa tgatttatag ggtctctcca atctgttttt ttaggtaccg
     1321 ccggagcttt actcaaggca catggaaata atttaaaacc atgcttttca tagaaatcta
     1381 tcacttttgt cacttccttt atattttatg ctaactttta ttgtaatact atacatgggt
     1441 aatgtccagt tgaatcggtg gattatgacc aattgtttgt tggtatctct ataaatgtgt
     1501 aataatggag atattagaga taatttagta tatattctgt gtcacatatc catatatata
     1561 gtatatctag taaagtttcc taacgaaaaa tattatatat ttaagaaaat aaaatattat
     1621 tataggaata gtttttagaa aagtagtaaa taagtggctt tgtgacacag attggaaaat
     1681 agacgatttt tggactgaat tgaagctgga taggggcaat gtgaaaatgg aatctccccc
     1741 gcatccgtga acagataaca cagcatcagg ttttccgatg ctgtgtttac tactctagta
     1801 gtgactgtct acttttccat acatagtcta cgtgttagct ctttttgaat cttctctaca
     1861 cgtacagtaa aatgttcaag aactaatgga ctattaccat acttcttttc atcttctata
     1921 gtacataaag actcattaag catgtctatt aagttgtttt ttgattgccc tttaagcagc
     1981 tcttcaaatg taactttcat accagtacaa tctccttaat aaaaatatcc ctgtctgcat
     2041 taaacatggt caggtccccg gctctcatct tcttgatcat cttccttgcc ggtaagccct
     2101 gcaacgtata aaacttggac ctctctccac gtttcacttc aattgttaat ttagtcatga
     2161 ctcttgctcc tcttgaatat taatattttc ctcttctata taccatgtta agcatggctc
     2221 ttgacaatc
//
LOCUS       DBNGSV010000103         1876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_849405, whole genome shotgun sequence.
ACCESSION   DBNGSV010000103 DBNGSV010000000
VERSION     DBNGSV010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_849405"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..909
                     /locus_tag="AC159U_03640"
     CDS             283..909
                     /locus_tag="AC159U_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336179.1"
                     /translation="MSVLTDWVKSNAKEGANLAEFEELLSGNTIDGIDSKEKALKLLY
                     EQKLLKAAFDSEISKKIASHDDRFKEEKLPSLLEAERERLRAELNPEETPEQKEMREL
                     RSEMQKIKAERDMSALKEKLRAKAKELNYDPDRAAKLAIIGDYDQASQMMTDQAEYLQ
                     STLEAERIKLVNDRLGGDPPKGGKALDSSLTEEKIASMSIAEIEREFK"
     gene            929..1777
                     /locus_tag="AC159U_03645"
     CDS             929..1777
                     /locus_tag="AC159U_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HIR3336180.1"
                     /translation="MALTNLTPELWSSIMQKYLDKNQVYGSLVNRDYQGEIRQFGDTV
                     HINSIGAVTISDYTKDTDSTATEVLSATDQTLIIDQAKKYDFTIDDVDGAQNKIQAAM
                     PALGRAGYGLADEMDSFLASLHTGVAAANIVGLGNDTTPVVPTKDTIYGYFVTARTLL
                     AEANVPPNQRWAVVPPWVMALIINSPEFISASNLGDEVKMSGAVGSIAGFMVYESNNV
                     PETTGTKYKVIFGHPWSITLAEQLMKPEVKRHYLRLGDQYRGLHLYGAKLVHSTGIAV
                     ATFNKA"
BASE COUNT          666 a          300 c          407 g          503 t
ORIGIN      
        1 atcagacaga aataaaatgc ttgaggcttt gatctggata tacaattccg aaggatggga
       61 agaactctta cctataatcg aaaaagcaac aggcaaatca tgggaagaaa taaaagcttt
      121 aagttaaaac atgtcattta tacaattcaa gctcctaaat aggggctttt tttatgctca
      181 aaacaagcta aatgcttaac ccttgataag taatcaaatg tatagtacaa tgtaactaaa
      241 ataaatatcg tttatggcta tatgctgtaa ggggagaact aaatgagtgt attaacagat
      301 tgggtaaagt ccaatgcaaa agagggtgca aacttagcgg aatttgaaga acttctgtca
      361 ggtaatacaa tcgatggcat tgatagcaaa gagaaagctt taaaactact gtatgaacag
      421 aaacttttaa aagcagcgtt tgattctgag attagcaaaa agattgctag tcatgatgat
      481 aggtttaaag aagagaaatt accaagccta ttggaagcag aacgtgaaag actcagagct
      541 gaattaaacc ctgaagaaac gccagagcaa aaagagatgc gagaattacg atcagagatg
      601 caaaagatta aggcagaacg tgatatgtca gcattgaagg aaaaattaag ggctaaagct
      661 aaagaactta attatgatcc tgatagagct gctaaacttg caattattgg agattatgat
      721 caggcttcac aaatgatgac cgatcaggcg gagtatttac agtcaacact cgaagcagag
      781 agaataaagc tagttaatga ccgtttgggt ggtgatcctc ctaagggcgg taaagcatta
      841 gatagttccc ttacagaaga gaaaatagcc agtatgtcta ttgccgaaat tgaaagggaa
      901 ttcaaataat aaataaaaga ggcataaaat ggcactaaca aatttaacac cggagctatg
      961 gagctcgata atgcagaaat acctggacaa aaaccaggta tacggatctt tagtaaatag
     1021 agattatcag ggtgagatta gacagtttgg cgatacagta catatcaata gtattggtgc
     1081 tgttactatt tctgactata caaaagacac tgactctaca gcaacagaag tacttagcgc
     1141 aactgaccag actcttatta tagatcaggc taagaagtat gactttacta ttgatgatgt
     1201 tgacggtgca cagaataaga tacaggcagc aatgccggct ctagggcgtg cgggttatgg
     1261 tttagctgat gaaatggata gctttcttgc ttcattacac acaggcgttg cagcagctaa
     1321 tatcgttgga cttggtaatg atactactcc tgtagttcct actaaagaca ctatttatgg
     1381 atatttcgta acagcaagaa cacttctggc agaagcaaat gtaccaccta atcagagatg
     1441 ggctgttgtt cctccatggg taatggcttt gattatcaac tctcctgagt ttatcagtgc
     1501 atcaaacctt ggtgatgaag ttaaaatgag tggagctgtt ggatctattg caggatttat
     1561 ggtttatgag tctaacaatg ttcctgaaac aactggaact aaatacaagg ttatttttgg
     1621 acatccttgg tcaattactc ttgctgaaca gctaatgaaa cctgaagtca agagacatta
     1681 tcttagactt ggtgaccaat atagaggtct acatctatac ggtgcgaaac ttgtacattc
     1741 aacagggatt gcagtagcaa catttaacaa agcataattt tatggggagc ttaaccgctc
     1801 ccctttatta aggagaatag catgtgggca gtaaataata ttacaggcgt taggtttgaa
     1861 atgacagaag attact
//
LOCUS       DBNGSV010000104         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_988791, whole genome shotgun sequence.
ACCESSION   DBNGSV010000104 DBNGSV010000000
VERSION     DBNGSV010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_988791"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..337)
                     /locus_tag="AC159U_03650"
     CDS             complement(62..337)
                     /locus_tag="AC159U_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HIR3336181.1"
                     /translation="MADQTINQSKIDFSNFVLSLNTSALIHLGDIPDPQTRERIYDIQ
                     SAKQTIDILELLKTKTDGNLDTEEFKLLDDVIYDLRMKYVKISQTDK"
     gene            complement(362..1333)
                     /locus_tag="AC159U_03655"
     CDS             complement(362..1333)
                     /locus_tag="AC159U_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR3336182.1"
                     /translation="MKGGKDYYNILGVSKSASADEIKKAFRNLARRYHPDINPGDKKS
                     EEKFKDIQQAYEILSNPEKKKSYDMFGEAGFQGGARPGGYQTHYSGNFGDLGENINFG
                     GASGFSSFEDIFGEIFGTRGGGRQHSQTRRPTKGRDVEHQIEIDFNTAIKGGTRDIKI
                     ARESNGKDYSTETISVKIPSGVDNGSKIRVAGKGEPGRNSGSRGDLYLRVKVAPHPIF
                     KRNKNDIFLDLPVTIFEAALGAEVSVPTVDSTATLKIPAGVQNGTKMRLKGKGVLNPK
                     TRERGDQYINIVVSMPDMMTQTDKKKFEELSESIKYNPRINIERYTD"
     gene            complement(1412..2470)
                     /locus_tag="AC159U_03660"
     CDS             complement(1412..2470)
                     /locus_tag="AC159U_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR3336183.1"
                     /translation="MSLNSDSIKSALKTVKYPGYTRDIVSFGIVKNIEVVDSNVKISL
                     VLPKPDPELEEQIKQAVKKTVLELPGVNGLEINSGARPPKDAPAKPTAEEPKENKLSN
                     IKYFIAVASGKGGVGKSTVAVNLALAASKLRKNVGLMDADIWGPSLPIMMGTKDKPTT
                     TDKKKLNPIEKYGINLMSIGFLISEDDTVIWRGPMVHSAIKQFLEDVDWKDTEYLFID
                     LPPGTGDSQLSLIQSAPITGGVIITTPQDVALLDVKRGVQMFRKLNVPVLGIVENMSH
                     LNCPHCSGEIDVFSRGGGFKMAEKFDIPFLGEIPIDPEIRKGGDYGNPIVLSQPESEV
                     SKSFTNIAEQILKIIEKD"
     gene            complement(2476..2877)
                     /locus_tag="AC159U_03665"
     CDS             complement(2476..2877)
                     /locus_tag="AC159U_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR3336184.1"
                     /translation="MKKIISIQQDKPASDGPQEVSFKKFEKKEPVDEHIEITEEMVYE
                     SLKGVNDPELPVNIVELGLIYDTQISGRDVKLKMTLTTPGCGMGAMIANQAEMAIKNI
                     GARNVMIEIVWDPPWNPDMMTDEAKQRLGVA"
     gene            3118..>3242
                     /locus_tag="AC159U_03670"
     CDS             3118..>3242
                     /locus_tag="AC159U_03670"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3336185.1"
                     /translation="MRRQTRQFSLGNVKIGGGAPVSVQSMTKTDTRDIDATVEQI"
BASE COUNT          840 a          690 c          578 g         1134 t
ORIGIN      
        1 ggaaaaaagt gctggaaatt ttatcaaaca gtgaaaaaat aattatgaag aattagccct
       61 cttatttatc agtctgactt atttttacat acttcattct cagatcatat ataacatcat
      121 cgagcagttt aaattcctca gtgtccaggt ttccatcggt cttggtcttt agcagttcga
      181 gtatatcaat tgtttgcttc gctgattgta tatcatatat tctctcacgt gtttgagggt
      241 ctggtatatc accaagatga attagcgcag aagtgttgag tgaaagtacg aaattagaaa
      301 aatctatttt tgactggtta attgtttgat ctgccattta ttcctccaaa atttgtctct
      361 attaatctgt gtatctttcg atgtttattc ttggattata ttttatggat tctgaaagtt
      421 cttcaaattt ctttttgtcg gtttgagtca tcatatcagg catggacact actatattta
      481 tatactggtc cccgcgctct cttgtcttgg ggttaagcac tcctttgcct ttaagcctca
      541 tttttgtgcc attttgaacc cctgcaggta ttttaagagt agctgtggaa tctaccgtgg
      601 gaacactaac ctcagcgccg agagctgctt caaaaatagt aactggtaaa tctaaaaata
      661 tatcgttctt atttctctta aatattgggt gtggggctac tttcactctt aagtacaagt
      721 cacctcgact accgctgttt cttccaggct ccccttttcc cgctacacga atctttgaac
      781 cattatcaac gcctgatgga atctttacag agatagtttc ggtggaatag tctttgccgt
      841 ttgattctct agcaatcttt atgtctcttg taccaccttt gattgctgta ttaaaatcaa
      901 tctctatttg atgttctaca tctcttccct ttgtaggtct tctggtttgg ctatgctgtc
      961 tgccgccgcc tctcgtacca aatatctcgc cgaatatatc ttcaaaactg gaaaatcccg
     1021 aggctccgcc aaagtttatg ttttcaccta aatctccgaa attgcccgag taatgagtct
     1081 ggtatccgcc gggacgcgcc cccccctgaa aacctgcttc gccaaacata tcataagact
     1141 tctttttttc aggatttgat aaaatctcat aagcctgctg aatgtcttta aatttttctt
     1201 cggatttttt atcaccgggg ttaatatccg gatgatatct tcttgcaaga ttcctaaatg
     1261 ctttctttat ttcatctgcc gaagcacttt tggacactcc taaaatattg taatagtcct
     1321 ttccgccttt catgttttaa acctgtaaat tatattaaat tacaataatc ataaaactaa
     1381 aatattcaag gacacaacat gctgaaaaaa atcagtcctt ctctattatt ttaaggattt
     1441 gttctgctat attggtaaat gatttggata cttcactttc aggttgtgac aggacaatag
     1501 gatttccata gtctccgcct tttctaatct caggatcgat ggggatttct ccaagaaaag
     1561 gtatatcaaa cttctctgcc atcttgaacc ctccgcctct gctgaaaaca tcaatttctc
     1621 cagagcagtg tgggcagttt aaatgactca tgttttcaac tatcccaagc accggaacat
     1681 ttaatttcct gaacatctga actccgcgtt ttacatccaa aagcgctacg tcctgtggag
     1741 ttgttataat tacccctcct gtaataggtg cgctttgtat aagtgagagc tgtgagtccc
     1801 cggttcccgg cggaaggtct atgaataaat attcagtatc tttccagtcc acatcctcta
     1861 aaaactgctt tatggcgcta tgaaccatag ggcctcgcca gattacggtg tcgtcttcgc
     1921 ttattaaaaa ccctatagac attaggttta tgccgtattt ctctatcgga tttagttttt
     1981 tcttatcggt agtggtgggt ttatcttttg tgcccatcat gataggcaaa ctgggtcccc
     2041 atatatcagc gtccataaga cccacgtttt ttctgagttt tgatgctgca agagcaaggt
     2101 ttactgcaac tgtggatttg ccaaccccgc cttttccgct tgctactgca ataaaatact
     2161 tgatgtttga tagcttgttc tcttttggtt cttcagctgt aggttttgct ggtgcatctt
     2221 tgggaggtct tgccccagag tttatttcca gaccatttac tcctggaagt tccagcacag
     2281 tcttttttac agcctgttta atctgctctt caagctcagg atcgggtttt ggcagaacaa
     2341 gagaaatctt tacattagaa tctactactt ctatgttctt tacgattcca aatgagacaa
     2401 tatcgcgggt atagcccgga tattttacgg tttttaatgc actttttata ctatcggaat
     2461 taaggctcat ctatattacg caacaccaag cctttgttta gcttcgtctg tcatcatatc
     2521 cgggttccat ggtggatccc aaacgatttc aatcataacg tttcttgctc cgatattctt
     2581 aattgccatc tctgcctgat tggcaatcat tgctcccata ccgcaacccg gggtggttag
     2641 agtcatctta agcttcacgt ctctgcctga gatttgtgta tcataaataa gccccagctc
     2701 tacaatattt acaggcagct cgggatcgtt tacgcctttt agtgattcat agaccatttc
     2761 ttcggtgatt tcaatgtgtt catcaacagg ttctttcttc tcaaactttt taaacgacac
     2821 ttcctgcggt ccgtcgcttg cgggtttatc ctgctgtatg gatattattt ttttcattcc
     2881 cataatacta cctccgttgt tatacttgtg ttactcttat atgtatatta tatcaacaaa
     2941 cttttaatgt ttctttaatt tagatgagtt atataaagat tagattaatt ttatgttgga
     3001 tgtactatat aaaataaaac cattttaatt gcatgtgtca atcttaatgc ctgcaatata
     3061 tagtaccata tccaatcttg acaccatatg gtatttaaac taacctagaa gcgcgaaatg
     3121 agaagacaaa caagacagtt cagtttagga aatgtaaaaa taggcggcgg cgctccggtt
     3181 tcggtgcagt ccatgacaaa gaccgataca agggatatag atgccacagt ggagcagata
     3241 ag
//
LOCUS       DBNGSV010000105        16186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_642527, whole genome shotgun sequence.
ACCESSION   DBNGSV010000105 DBNGSV010000000
VERSION     DBNGSV010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16186
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_642527"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="AC159U_03675"
     CDS             complement(<1..207)
                     /locus_tag="AC159U_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3336186.1"
                     /translation="MENKRNSRRIKFKERIKYGASDIPNTDGTTVNISPKGIAIKSYK
                     ALTPGSKISVVLYSGNNPIRLEGEV"
     gene            complement(308..757)
                     /locus_tag="AC159U_03680"
     CDS             complement(308..757)
                     /locus_tag="AC159U_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3336187.1"
                     /translation="MQVKEIMVSPVVSVKPDDTLEDVARIMIKNRIGCVPVVGKDSEL
                     LGIITENEFSVNEKRFPFSREYAPKLFGMWVTKDNIDKMYDAAKTIYAKDLMYQNPVT
                     VDMEDSVFDLLKKMIDQSVDRVPVLNNNRVIGIVARHDLLKLIIDNN"
     gene            complement(782..1858)
                     /locus_tag="AC159U_03685"
     CDS             complement(782..1858)
                     /locus_tag="AC159U_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HIR3336188.1"
                     /translation="MALRLIELYLPTKENIDWLKEKLSDYPVHGIWSERVSDHQYHIR
                     MLISPEDSGAILDLLENTFSTVDGFRIILLPVETTIPKIEEKKQEAENDSQKKKNDEI
                     KIGRGISRQELYTRISKDSKLTGFFIFMVIVSSIVASIGILNSNIAVIIGAMVIAPLL
                     GPNVALALATTLGDLDLLKNCIKSNLAGISAALLFSLILGILLRNKLAIDTDLKEFYP
                     ELYSRTQVGMGDIALALASGSAGALSFTMGLPSALIGVMVAVALLPPLVTFGMLIGAG
                     LIEPAIGAILLLLVNIICVNLAGVGTFLFQGVRPRTWWEASRAKKSSRIAIIIWTVLL
                     LILMIIITQLKNNFFDKIFKIILP"
     gene            complement(2012..2965)
                     /gene="trxB"
                     /locus_tag="AC159U_03690"
     CDS             complement(2012..2965)
                     /gene="trxB"
                     /locus_tag="AC159U_03690"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3336189.1"
                     /translation="MSDKEQLKKRYKVVIMGSGPAGLTAALYSARANLEPLVFEGFEA
                     GGQLTLTTDVENYPGFENGIMGPDLINTMRKQAERFGAKCLLSNIDSVDLSKRPFKIK
                     AGDVEVEAESFIIASGASARMLGLPSEKELVGHGVSTCATCDGFFFKDKEIIVVGGGD
                     SALEEGTFLTKFASKVSIVHRRDSLRASKIMQDRAFKNEKVEFIWNTVVEEILGEKGR
                     GVTGVKLKNVETGDITEKNVDGVFIAIGHIPNTTIFNGAIDTDENGYIVTKNKTSETN
                     IPGVFAAGDVQDTRYKQAITAAGSGCMAALDAEKFLEDNED"
     gene            3111..4184
                     /gene="ygfZ"
                     /locus_tag="AC159U_03695"
     CDS             3111..4184
                     /gene="ygfZ"
                     /locus_tag="AC159U_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3336190.1"
                     /translation="MKKLPLHNFYLEKGAEFVESGGCTVPKHFDSIDNEYQAARDAVA
                     VYDLSARGKIRLEGKECFKFMQGMLSNDVMKLESGKGVHATLLTVKGKMLADLYIYKD
                     GEQALIDLEQGLNVPISELLLKYRLSYKAKIEDLTDKYVQLCFFGAKSQQYLSRLLDL
                     DLSDLDEPGLIKKSIDDIEIIIVKVKRSSNESFDVYIPTGETNWVIQFFFGQIDGLTP
                     KPIGLDTFEILRVQAGIAKYGVDMDENTIPIEAELWDALSFEKGCYIGQEVIARIKWR
                     GHVNRHLVGIEMQTNTPAHPGDKIFVNDKEVGYVTSSVYCYEKTKAICMAYIKREFKE
                     PGTAVIVSINDENIDGKVFSFNN"
     gene            4307..5344
                     /locus_tag="AC159U_03700"
     CDS             4307..5344
                     /locus_tag="AC159U_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3336191.1"
                     /translation="MLFDSIIGMFSNDLAVDLGTANTLVYVKGKGIVGNEPSVVAVQE
                     ELRGPKKILAVGKEAKDMVGRTPGSIMAIRPLKDGVIADFDVAQKMLEYFIIKTHNNR
                     KSFVRPRIIVSVPIGITEVEKRAVKESAEAAGAREVYLMEETMSAALGAGMPVTEPTG
                     NMVVDIGGGTTGVAVISLAGIVVSKSVRVGGDKLDEAIMQYVKRNYNMLIGERTAEDI
                     KIKLAQLYGNGEIKTMQVKGRDLRAGVPKTIDITSDEVREALSEPIGVMVEAIKQTLE
                     RTPPELAADIVDNGIMLTGGGALLGGLDKLLMQETGLPVAVAEDPLSCVVYGSGKALD
                     ELDLLKEVSLN"
     gene            5413..6258
                     /gene="mreC"
                     /locus_tag="AC159U_03705"
     CDS             5413..6258
                     /gene="mreC"
                     /locus_tag="AC159U_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3336192.1"
                     /translation="MGFLRRYPLFVVLIMIFFLVQLVPLNNENKLNNSRFSEIILTIT
                     YYPQKSINSLSRYIVQKWNKYVDNVDQYEQNRVLKKDLAKLREENFRLSEIRLQNKRL
                     RELLNFRAGEQYEVIAANTVAISASAIKSQVIIIDKGAVDGLVKGMPVKTHQGAVGKL
                     YIVGKYSSEVLLITDSLSAVDAYVQRSRERGVVKGDGNRCIMDYLEKASDIAVGDKIV
                     TSGKDGYFPKGILLGEVDEIFSESGNIKAKIEPQIDINSVEEVLIINKKPREFVIPEQ
                     QVVNE"
     gene            6251..6742
                     /gene="mreD"
                     /locus_tag="AC159U_03710"
     CDS             6251..6742
                     /gene="mreD"
                     /locus_tag="AC159U_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3336193.1"
                     /translation="MNRLYVYLFFSIVLFIFVILQTTLLSPVNLGRLYIDFTLILIIY
                     LSLNTDVRGGVFFAFLSGYIIDLYSGTNLGMYVFSRLSLFVIIRFLVTKIYSDRFDIQ
                     ILLIFFSIIYEWVLLSAIFSFSSQLDTALSYNFIIINILVNTFAGYICFIILKELHGK
                     LHT"
     gene            6723..8525
                     /gene="mrdA"
                     /locus_tag="AC159U_03715"
     CDS             6723..8525
                     /gene="mrdA"
                     /locus_tag="AC159U_03715"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3336194.1"
                     /translation="MENYTRRFLVSVFILIFFFMVLGIRIWYLQILKGDEFEKFSRNN
                     RIRNIRVVPPRGRILDRNGRGIVINRSSFDLYAVPSEIKDRETFLAFLSAVLLLDTEE
                     LEDRIYGSSDNKKFNQVLIERDITRDQLAVLESRRHTYPWMFIEVSKIREYPYRKLGA
                     NFIGFVGKTTKTELKTHTDLTGFDLIGKSGLEKHYQSYLKGSPGYIQKVTDAFGREIK
                     SSVYRSDINLQQSIPGADIKLTVDIDFQVAVEQILSDYTGAAVVMNVENGEILAMASS
                     PTFDPSIFVKGVDRKDWKELTSDASYPLLNRVTRGLYPPGSVYKIVTAAAALEENAID
                     VDTRFYCPGHYTLGTKTFRCWKSSGHGWVDIHKALVESCDVFFYEVSQKIGIDKIDKY
                     SRQMSYGTFTGIDIDEKSGISPSKTWKKERLKAPWYKGDTVNVSIGQGFLSVTPLQVA
                     VMTSSIANGGYKVKPHFIKEVVSNTGEQLYSYKDEIEQISVADNNISIISDAMAGVVN
                     QQNGTARYARLPHIRVAGKTGTAQVVALDSAKLKNKKYDHHAWFTAFAPADNPEIAVT
                     VLVEHGGKGGAVASPLAKKIIEAYYNPNFHGPRL"
     gene            8522..9643
                     /gene="rodA"
                     /locus_tag="AC159U_03720"
     CDS             8522..9643
                     /gene="rodA"
                     /locus_tag="AC159U_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3336195.1"
                     /translation="MINFRLLKNFDVPLFFITIIICAIGIINLYSATINIGTAIFKKQ
                     IFWVSLGLIITVILSLFDYRMIQRYSNHFYLINILLLVIVIIFGREVSGARSWLSLGG
                     AVNIQPSELTKLAVIFVLAKFYHDDFYEGLYSVSELVKPFLMAVIPIVLIALQPDMGT
                     AFIVTLIALSIILFAGVKTKHIILLTICGSLLFVPLWGYILKDYQKTRIKAFLDPSLD
                     PLGSGYHATQSKIAIGSGKLSGKGFTQGTQTQLRFLPEQHTDFAFSVIAEEWGFLGSI
                     FTILLFLILILWILDTSSRSKDKFSMYVCFGIASMFFWHTFINIGMVIGIFPVTGLPL
                     LFISYGGSASLTSLIAIGIVMGIRMRKSRVSEQDFSFND"
     gene            complement(9655..10614)
                     /locus_tag="AC159U_03725"
     CDS             complement(9655..10614)
                     /locus_tag="AC159U_03725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3336196.1"
                     /translation="MGLITDIKGIKVGHCSDFENITGCTVVLFDGNATGTVDMRGGGT
                     STRQIDGLLPHHNFSNINAIMLTGGSAYGLDASSGVMKYLEEKLIGLKVGSSSVVPSV
                     PTAVIFDLGIGNSKVRPNGDFGYKACQNAKKRFEQGTVGVGTGATIGKYLGINHATKG
                     GLASESLELPNGTKIGVLVVVNAFGSVYDYKDGNTCIAGIRNTKGKNFLDTSDLIHKG
                     VPQTTNTIRNTTLAVVATDAKFSKEQLNRIAVIAHTGISRVINPCHTISDGDMVFAVS
                     MGNKEAKINDVGIMASELISDSIIKAVKSASGLGGLPSYSDLI"
     gene            10783..11622
                     /locus_tag="AC159U_03730"
     CDS             10783..11622
                     /locus_tag="AC159U_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3336197.1"
                     /translation="MLILLMFLLVLVGGTGNSFAINDVKDLKQVGIDEHLGDYIPLDL
                     EFYDTNNKKVKLENYFLEKKPVILSLVYFSCPRVCNYATDGVVDVINRVSSLNLGRDY
                     KILTVSFNAEDNPQIAGEKIKRYYDLLNEQHFPKGNWHFLTGNSDSITKLTESVGFKF
                     KKEGEEFAHPSSLIIITPDGKISRYLNGIQHEPKDLKMALLEATNGEIGTSKIINKAL
                     LFCYQFDPVGKKYALQALNVVKAGGVVTLLSLGVFLTYFWKREKKISRKDGGFDDRNE
                     LGS"
     gene            11606..12547
                     /gene="coxB"
                     /locus_tag="AC159U_03735"
     CDS             11606..12547
                     /gene="coxB"
                     /locus_tag="AC159U_03735"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3336198.1"
                     /translation="MNWVPDVASNFANQVDGVLWFITWLSIIFFILITILLVYFSFKY
                     KRKTENDETPHITGNETLEIIWTIIPSALLMVIFVYGFVVYKDMRTPPPEAVEVNVTA
                     KQWLWVFKYPNGQSTINELYVEHNRPVKMVMRADDVLHSFFVPAFRVKQDVVPGMYTQ
                     LWFKPTKVGTYDLFCTEYCGKDHSNMLAKVYVMSPEAFARWERGGDEEEGIGAVVAKS
                     PEELGQKLYEKNGCAACHTLDGSNSVGPTFKGLFGKTEPLQDGTSVLADENYLRESIL
                     LPQEKIVAGYAPVMPSFKGLLKDEEVDAIIAYIKTLK"
     gene            12579..14186
                     /gene="ctaD"
                     /locus_tag="AC159U_03740"
     CDS             12579..14186
                     /gene="ctaD"
                     /locus_tag="AC159U_03740"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR3336199.1"
                     /translation="MANGTAATMSEPSFLEYKTGFMSWAWTTDHKRIGILYLISVTFF
                     FLVAGAIAVIMRTELFTPESDLVSPHTYNVLFTLHGSLMVFFFIVPGVAASLGNFLIP
                     LMIGAPDVAFPKLNMASYYVYIAGTIITLIALLQPADTGWTFYTPYSAKTTTNVILIT
                     FGVFVLGFSSILTGMNFIVTIHKMRAPGMTWHRLPLFIWASYATAILQLLATPVVGIT
                     LLLLIAERTLGVGFFDPDKGGDPILFQNFFWFYSHPAVYIMIIPAMGVISEILPVFSR
                     KPIFGYKAIAYSSVAIALISFLVWAHHMFVSGISETAATIFSFLTFLVAIPTAIKVFN
                     WIATLYKGSIEFNSAMLYALSFIFLFTIGGLTGLHLGALASDIHLHDTYFVVAHMHYV
                     MIGGTVIGFFGALHFWYPKWFGRMLDETLGKIAWFLVFIGFNVTFFPQFFLGVQGMPR
                     RYASYLPEFEPLMKLSTIGAWILAIGIGIMVYNMIKSLYSGEKASDNPYGSLSLEWQT
                     SSPPIHENFHEVPVVTDWTYGYGKKEG"
     gene            14210..14818
                     /locus_tag="AC159U_03745"
     CDS             14210..14818
                     /locus_tag="AC159U_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HIR3336200.1"
                     /translation="MSEITAGHGHGHIQIDPVEEYKQSKFGMWLFLGTEILLFGGLFA
                     AYAIFRAKYPEMFHEQHQQLDWQLGALNTVILLFSSMTMAIGVTASKRGNNKLLIAML
                     AITIICGLAFGYNKYIEYGYKFSHHIYPDKSIFYSLYFLMTGLHMIHVFIGLVILLVV
                     MVYAIRGVYSAEYNTHVEVSGLYWHLVDLIWIYLFPLLYLIG"
     gene            14836..15135
                     /locus_tag="AC159U_03750"
     CDS             14836..15135
                     /locus_tag="AC159U_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3336201.1"
                     /translation="MSEHHEEHITPIKTYFIIFGILIALTVITVYTAKEVDLGAFNFF
                     LAMIIASAKATIVALYFMHLKFEDSITWVFAIYPLFLLGLLIGLTSVDIFNRITP"
     gene            15156..16022
                     /locus_tag="AC159U_03755"
     CDS             15156..16022
                     /locus_tag="AC159U_03755"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3336202.1"
                     /translation="MLEKSTVGLFFLLLIWGSVVTGLEAGLACPDWPKCHGEYIPPLR
                     WDIYVEFSHRVLGGVAGLLLIILAYKRFKVYDQAYRAIPIIAVVLLAIQVVMGGLVVL
                     LKLPMNLTTLHFSTALIIFSLVLFLAYFNDSRKPDFSNNTFTLFFFLSLLVLSQGALG
                     AYVRHSGAGLACPDFPKCLGFWIPPELSGIVLTHFLHRTLAYIIFAVFIFLLIYSRTT
                     DTLQKYRKKIAIVFGLIIFQILLGVGIIHTKLYFAVTATHVFVALLILYILLNTWYSE
                     NTKHSENAGSTV"
BASE COUNT         4890 a         2874 c         3265 g         5157 t
ORIGIN      
        1 aacttcacct tcaagtctta ttgggttatt gcctgaatat aaaacaacag atattttaga
       61 acccggagtg agtgccttat aggattttat tgcaattcct tttggggaaa tatttacggt
      121 agtaccatca gtattaggta tatccgatgc accatatttg attctttctt taaactttat
      181 tcttctacta tttcttttgt tttccatagt aaataaagaa taattttaga gtttaatata
      241 attctattgc ccttagtgaa ataattcaac cgaatacggt ctaaagacag aaaaactagt
      301 ctaaagtcta attattatct attattagtt ttagaaggtc gtgacgggca actatcccta
      361 ttactctgtt attatttaat acaggaacgc ggtcaacaga ctggtctatc atttttttga
      421 gaagatcaaa tacggaatct tccatatcta cagttactgg attttgatac attaaatctt
      481 tggcataaat tgtttttgca gcatcataca ttttgtcaat attgtccttt gttacccaca
      541 tgccaaacaa tttaggagca tattcacgtg aaaaaggaaa tcttttctca ttcacactaa
      601 attcattctc tgttattatt cctagaagct cagaatcttt acctacaacg ggcacacagc
      661 ctattctatt ttttatcatt attctggcaa catcttccaa ggtgtcatca ggttttacag
      721 atactacggg tgagaccatt atttctttta cttgcataaa tcctccaaat taataagtaa
      781 tttaaggcaa tattatttta aaaatcttat caaaaaaatt atttttaagc tgagttatta
      841 ttatcataag aattaataag agtacagtcc agataattat tgctatcctt gatgattttt
      901 ttgccctgct tgcttcccac caagtacgtg gtcttacacc ctgaaataaa aaagttccaa
      961 cacctgcaag atttacgcat ataatgttta ctaaaagcaa cagaatagct ccaattgcag
     1021 gttctataag gccagcgcca ataagcatgc caaaggttac gagcggcggc aaaagcgcta
     1081 cagcaaccat cactccaata agggcacttg gaagccccat ggtaaatgaa agcgcaccag
     1141 cactccccga tgcaagagca agtgcaatat ctcccatacc aacctgtgtt cttgaataca
     1201 gttctggata aaactctttt agatccgtat caattgcaag tttatttctt agtaaaatcc
     1261 caagaattaa tgagaataat aaagcagcag aaattccggc cagattggat ttgatacagt
     1321 tttttagtaa atccaagtct ccaagcgtag tggccagggc aagggcaacg tttggtccca
     1381 gaagtggtgc aataaccatt gctcctatta tcacggcaat attactgtta agaatgccga
     1441 tagatgcaac tatggatgat actataacca tgaaaataaa gaatccggtg agtttggagt
     1501 cttttgaaat tcttgtatac agttcctgcc tgcttatgcc tctaccgatt ttgatctcat
     1561 catttttttt cttttgagaa tcattttcgg cttcctgctt tttttcttca atctttggga
     1621 ttgttgtttc aacgggcagc agaattatcc tgaaaccatc aacagtagaa aaagtatttt
     1681 ccaatagatc gagaattgct ccagagtctt caggtgaaat gagcatacgt atatggtact
     1741 gatgatcgga aaccctctca ctccaaatac catgaacagg ataatcgctt agcttttctt
     1801 taagccagtc gatgttttct ttagtaggta gatatagttc tattagtctt agagccatat
     1861 gaatttaata tatctgcaat aaatctattt tgatctattg taacatagtc aattccaagc
     1921 tctaggttta agaagttaaa tagatggatt tagccttatt gggtttaatg cttggctata
     1981 gtaatgaact tgtgatagct tagtaataaa cctaatcctc attatcttct aagaactttt
     2041 cggcatcaag tgcagccata catcctgacc ctgctgctgt gattgcctgt ttgtaccttg
     2101 tgtcctgtac gtcacctgct gcaaaaactc cggggatgtt tgtttcgctg gttttattct
     2161 tggtgactat atatccgttc tcatcggtat ctatagcgcc gttaaatatc gtggtgttgg
     2221 gtatgtgtcc aattgcaata aaaactccat caacattttt ttctgtaatg tcgccggttt
     2281 ctacattttt aagcttaacc ccggtaactc ccctgccttt ttcaccgaga atttcttcca
     2341 caaccgtatt ccagataaat tcaacctttt cgttcttaaa ggctctgtcc tgcattattt
     2401 ttgatgctct caagctatca cgcctgtgaa ctattgatac cttggaggca aacttggtaa
     2461 gaaaagttcc ttcttcaagc gcgctgtcac caccacccac tactattatt tccttatcct
     2521 taaagaaaaa tccgtcacat gtagcacagg tcgaaacccc atgtcctacg agttcttttt
     2581 ctgaaggaag cccgagcatc cttgcagaag ctcccgaagc tatgatgaaa ctttctgctt
     2641 ctacctctac gtctccagcc ttaattttaa acggcctttt tgaaagatct acagaatcaa
     2701 tattggaaag aaggcatttt gcaccaaacc tctcggcttg ttttctcata gtatttataa
     2761 ggtccgggcc cataatcccg ttttcaaatc cggggtagtt ttcaacatcg gttgtaagag
     2821 taagctgacc ccctgcctca aagccttcaa acaccaaagg ttccagattt gctctagcgc
     2881 tgtatagtgc ggctgtaaga cctgccgggc ctgatcccat gattactact ttgtaacgtt
     2941 ttttaagttg ctctttatca ctcatatttt gtcctttata aataaatttt atactacata
     3001 tgtggcaatt attttcaagc tatctatatt aatgtctata ttttgaataa tttattctca
     3061 attagattca aagaatggga tttaatttta tattaaaaca ggagcaataa atgaaaaagc
     3121 ttccgcttca taatttttat ctggaaaaag gagcagaatt tgtagaatca ggcggctgta
     3181 cagtaccaaa acattttgat tctatagata atgaatatca ggcagcccga gatgcagtag
     3241 cagtatatga tctatcagca agaggtaaaa taagactgga gggtaaagaa tgttttaagt
     3301 tcatgcaggg tatgctctca aacgacgtta tgaagcttga gagtggaaaa ggggttcatg
     3361 caacccttct taccgttaaa ggcaaaatgt tagcagattt atatatttac aaagacggtg
     3421 aacaggcact aatagattta gaacaagggc taaatgtacc gatatcggag cttctgctta
     3481 aatacaggct ttcatataag gctaaaattg aagacttaac agataaatat gttcagctgt
     3541 gtttttttgg tgcaaaatca caacaatatt tgagccgctt attggatttg gatttatcag
     3601 atttggatga gccgggatta attaaaaaaa gtattgatga tatagagata attatagtaa
     3661 aagtaaagag gtcatcaaac gagagcttcg atgtctatat cccaaccggg gaaactaatt
     3721 gggttataca atttttcttt ggtcaaattg atggtctaac ccctaagccc atcggactcg
     3781 acacttttga aatcctaaga gtacaagcgg ggattgcaaa gtacggggtt gatatggatg
     3841 aaaatacgat acctattgaa gctgaactct gggatgcgct tagttttgaa aagggctgct
     3901 atattggcca ggaggttata gcaaggatca agtggagggg acatgttaac agacacctgg
     3961 tgggaataga aatgcaaact aacactcctg cacatccggg ggacaaaata tttgtaaatg
     4021 ataaagaagt gggttatgtc acaagctctg tctattgtta tgaaaagact aaggctattt
     4081 gtatggctta tataaaaagg gaatttaaag agcccggcac agcggtgatt gttagcatta
     4141 atgatgaaaa tattgatgga aaagtattca gttttaataa ttaaagtaga aatttttaaa
     4201 tatctgtagt tagatactat taattcttaa tatttaattt ataataacaa tgttttacgg
     4261 ggtgcattta tattttttga attactaagg aggggtttac taagaaatgc tatttgattc
     4321 aattataggt atgttttcca acgatcttgc agttgatctt ggaacagcaa atactcttgt
     4381 ttatgtaaag ggaaagggaa ttgtaggcaa tgagccttct gttgttgcgg tacaagaaga
     4441 attaaggggt ccaaagaaaa ttctagcagt tggtaaggaa gccaaggaca tggttggtag
     4501 aactccgggt tcgatcatgg caataagacc tctaaaggac ggagtgattg ctgatttcga
     4561 tgttgcccaa aaaatgctcg agtattttat tattaaaacc cataataaca gaaagagctt
     4621 tgtaagaccc aggattattg tatctgttcc aatagggata acagaggttg aaaagagagc
     4681 ggtaaaagaa tcagcagaag cagcaggggc aagggaagtt tatttaatgg aagaaaccat
     4741 gtcggcagca ctcggagctg gtatgcctgt tacagaaccc acagggaata tggtggtaga
     4801 tatcggaggc ggtacgacgg gtgtagctgt gatatcactt gcaggcatcg tagtgagtaa
     4861 atcggttaga gttggtggtg ataagctaga tgaagcaata atgcagtatg taaaaagaaa
     4921 ttataatatg ctcataggtg agcgcactgc tgaagacatc aagatcaaac tagcccagct
     4981 ttatggaaat ggtgaaataa aaacaatgca ggtgaaagga agggatttga gagccggggt
     5041 gccaaagaca atcgatatta catcagatga agtaagagag gcacttagtg agcctattgg
     5101 tgtaatggtc gaggccatta agcaaaccct tgaaagaacc ccgccggagc ttgctgcgga
     5161 tatagtagat aatgggatta tgcttacagg gggcggcgct cttctgggtg gtcttgacaa
     5221 actgcttatg caggagacgg ggcttcctgt ggcagttgcc gaggatcctt tgagctgcgt
     5281 tgtttatggc tcgggcaagg cgcttgatga gcttgattta ctaaaagaag ttagtttgaa
     5341 ttagattata ttagcagatt attaagtttc tttctttcca aactctaatt tctgcttaat
     5401 atattaatta gtatgggatt tctaagacgc taccctcttt ttgtagtttt aataatgatt
     5461 ttcttcctgg tacagctagt cccccttaat aatgaaaaca agttaaacaa ctccagattc
     5521 tccgagataa ttcttaccat tacgtactat ccccaaaaaa gtataaacag cttaagcaga
     5581 tacattgtac aaaaatggaa caagtatgtt gataacgtag accaatatga acaaaacagg
     5641 gtcttaaaaa aggatcttgc aaaactgagg gaagaaaact tcagattatc agaaatcaga
     5701 ctacagaata agaggcttag agaactcctt aattttaggg ctggagagca atatgaagtg
     5761 attgcagcca acactgtggc tataagtgct tcagctatca aatctcaggt gataataata
     5821 gataagggtg ctgtagacgg tcttgtaaaa ggtatgcccg ttaaaacaca tcagggtgct
     5881 gttggaaagt tatatatagt aggaaagtat agttcagaag tgctgcttat aacagactca
     5941 ctaagcgctg tagatgcata tgtacagaga agcagggaaa ggggagtagt gaaaggcgat
     6001 ggaaatcgct gtatcatgga ctatctcgaa aaggcatccg atatagctgt aggtgacaag
     6061 attgtaacat ccggtaagga tggttatttc ccaaagggta tactccttgg tgaggtagat
     6121 gaaatatttt cagaaagtgg gaatattaag gctaaaatag aacctcaaat tgatataaac
     6181 tccgtagaag aagtactcat cattaacaaa aaaccaagag aatttgtaat accggagcag
     6241 caggtagtaa atgaatagat tatacgtata cctgttcttt agtatagtac tttttatatt
     6301 cgttatttta caaactacac tattaagtcc tgtaaatctc ggcagactct atatcgattt
     6361 tactcttatt ttaataatat atttatccct taacacagat gtgagggggg gagtgttttt
     6421 tgctttttta agcggatata ttatcgatct ttactcaggc acgaatcttg ggatgtatgt
     6481 ttttagcaga ttaagccttt ttgttatcat aaggttttta gttacaaaga tatattcgga
     6541 cagatttgat attcagatac tgttaatctt ttttagtatt atatatgaat gggttttact
     6601 ttccgcgata tttagtttta gctcccaatt agatacagca ttatcgtata atttcattat
     6661 aattaacata ttggttaata catttgcagg ctacatatgt tttataatct taaaagagtt
     6721 acatggaaaa ttacacacgt agatttttag tatcagtttt tatactaata tttttcttta
     6781 tggttcttgg aataaggatc tggtacttac agatactaaa aggtgatgaa tttgaaaaat
     6841 tttccagaaa taaccgtata agaaacataa gagtagtacc tccaagaggc cgtattctgg
     6901 acaggaacgg aagaggaatt gtaataaaca gatcgtcatt tgatctttat gcagttccta
     6961 gtgagataaa ggatagagaa acttttcttg cgtttctttc cgccgtacta ttactggaca
     7021 cagaggagct ggaagaccga atctatggtt catctgacaa taagaaattc aatcaggttt
     7081 taatcgaacg tgatattacc agagatcagc ttgcagtcct ggaatcaaga cgtcacacat
     7141 atccatggat gtttatagaa gtaagcaaga taagggaata tccgtacagg aagcttggcg
     7201 ctaattttat aggatttgta ggtaaaacga caaagacaga actaaagaca cataccgact
     7261 taacagggtt tgatctaatt ggtaaaagtg gtcttgaaaa gcactatcaa agctatttaa
     7321 aaggaagccc ggggtatata caaaaagtta ctgatgcatt tggaagagaa attaaatcga
     7381 gcgtttaccg ctcagatata aacctgcaac aaagcatccc tggtgcagat attaaactta
     7441 ctgtagatat tgattttcag gtagctgttg aacagatact aagcgattat accggagctg
     7501 cagttgtaat gaatgtagaa aatggtgaga ttctagcaat ggccagcagc ccaacatttg
     7561 atccttccat attcgtaaaa ggggtagacc gtaaagactg gaaagagctt acgtccgatg
     7621 ccagctatcc tcttctaaac cgtgtcacca ggggtttata ccctccggga tccgtatata
     7681 agatcgttac tgcagccgca gcactagaag aaaatgcaat tgatgtagat accaggtttt
     7741 actgccctgg acattatacg cttggaacga aaacatttag gtgctggaaa tcttcgggcc
     7801 atgggtgggt ggatattcac aaggcccttg ttgagtcctg tgatgtattt ttctatgaag
     7861 tatcacagaa gatcggtata gataaaattg ataagtactc caggcaaatg agttacggta
     7921 cgtttactgg aatcgatatt gatgaaaaat ccggaatatc tccgagcaaa acctggaaaa
     7981 aagagcggtt aaaagctcca tggtataagg gtgatacggt taatgtatcc ataggtcagg
     8041 gatttttaag tgtaacaccg cttcaggttg cggtaatgac ttcatcaata gcaaacggcg
     8101 gatacaaagt aaaaccacat tttataaaag aagtggtttc gaatacagga gagcagctct
     8161 acagttataa ggacgaaatt gagcaaatat cggtagctga caataacata tcaattataa
     8221 gtgacgccat ggcaggggta gtaaatcagc aaaacggtac agcaagatat gcaagacttc
     8281 ctcatatacg cgtagcggga aaaaccggca cagcccaggt agtagcgctt gactcggcaa
     8341 aattaaagaa caagaaatat gatcatcatg cctggtttac agcatttgcc cctgctgata
     8401 atcccgagat agctgttacg gttttagtcg aacacggcgg taagggaggc gcggttgcct
     8461 cacctttagc taaaaagatt atagaggcct attataatcc aaattttcac gggccaagat
     8521 tatgataaat tttagacttc ttaaaaactt tgatgtaccc ctgtttttca taacaataat
     8581 aatatgcgca ataggcataa taaatttgta cagtgcaact attaacatag gaacagctat
     8641 ttttaaaaaa cagattttct gggtatccct tggtctcatt atcactgtaa tcttatcatt
     8701 atttgattat aggatgattc agcgctattc gaatcatttc tatttaataa atattttact
     8761 ccttgtgatt gttattatat ttggaagaga agtatccggg gcaagaagct ggctatcact
     8821 tggaggtgct gtaaatattc agccctccga gcttacaaag ctagccgtaa tatttgtact
     8881 tgcaaaattc taccatgatg atttttatga aggtctgtat tcagtttctg aactggtaaa
     8941 accattttta atggcagtta ttcccattgt tctcatagct ttacagcctg atatgggtac
     9001 agcatttata gttaccctta tagctcttag tataatatta tttgccggtg ttaaaacaaa
     9061 acatattatt ctactaacaa tatgtggatc tttattattt gttccactgt ggggttatat
     9121 attaaaggac tatcaaaaga caagaattaa agcttttctt gatccctcat tagacccact
     9181 tgggagtggt tatcatgcta cacaatccaa aatcgcaatt ggttctggta agttaagtgg
     9241 taaaggtttt acacagggca cacaaactca gctgagattt cttcctgagc agcacactga
     9301 ttttgccttt tctgtaattg ctgaggaatg ggggtttctt gggtctatat ttaccattct
     9361 gctattttta atactcatat tatggatact tgatacatcg agccgatcga aggataaatt
     9421 ttcgatgtat gtgtgcttcg gaatagcttc aatgttcttc tggcacacct ttattaacat
     9481 aggaatggta atcggaatct ttccggtcac aggccttcct ctgctcttta ttagttacgg
     9541 ggggtctgca tctcttacat ctttaatagc cataggaata gtgatgggca ttagaatgag
     9601 aaaatcaaga gtttccgagc aggatttttc atttaatgat tagttaatgg tagatcaaat
     9661 aaggtctgaa taagacggca gtccgccaag tcctgaagca gacttaactg cttttattat
     9721 tgaatctgaa attagctctg atgccataat acctacatcg ttaattttag cttctttatt
     9781 tcccatagaa actgcaaata ccatatctcc gtctgaaatt gtatgacatg gattaataac
     9841 tctcgaaatt ccggtgtgtg caataacagc aattctattt agctgttctt ttgagaactt
     9901 tgcatctgtt gctactaccg caagggttgt gtttctgatt gtatttgtgg tctgtggtac
     9961 ccccttgtgg attaagtctg aggtatctaa aaaattttta cctttagtat tccttatacc
    10021 tgctatgcat gtattaccgt ctttatagtc gtaaacactt ccaaatgcgt ttactacaac
    10081 taacacacca atttttgtcc catttggaag ttcaaggctt tctgaggcaa gcccgccttt
    10141 tgttgcatga ttaatcccta agtatttacc gatagtagca cctgtaccaa cacctactgt
    10201 cccctgctca aaacgcttct ttgcattttg gcaagccttg taaccaaaat caccattcgg
    10261 tctcactttg ctgtttccta ttcccaaatc aaatattaca gctgtaggta cactgggcac
    10321 aactgaactg cttccgactt ttaatcctat tagtttttct tccagatatt tcatcactcc
    10381 ggaagaagca tccagcccat atgcacttcc acctgtaagc attattgcat taatattact
    10441 aaaattgtgg tgtggcagca gaccatctat ctgtcttgta cttgtacctc cgcctctcat
    10501 atccacagtc cctgtggcat ttccatcaaa aagtactacg gtacagcctg taatattttc
    10561 aaaatcggag cagtggccga cttttatgcc ttttatatct gtaattagtc ccattttaag
    10621 taaaatatat catacagaca ctgtaaatag taagagtttt tgtgagaagg gtataattaa
    10681 tagtggtact tgacagggat tgataatcca catagaatct aattcctttt tgataataat
    10741 tcactataaa agttgcagat tatgttaaag aagttgtgta atatgttaat tttattgatg
    10801 tttttgcttg tacttgtggg tggtacaggt aactcctttg ctattaatga tgtaaaggac
    10861 ttaaagcagg ttggtataga tgagcatttg ggtgattata tacctcttga tctggaattc
    10921 tatgatacta acaacaagaa ggtcaagctt gaaaattatt ttctcgaaaa gaaacccgta
    10981 attcttagcc tggtctattt tagctgccca agagtatgta attatgcaac cgatggggtg
    11041 gttgatgtta taaaccgtgt atcctcatta aacctcggaa gggactataa gatcttaaca
    11101 gtcagtttca atgctgaaga caatccccag atagctggtg aaaaaataaa gcgctactat
    11161 gatttactaa atgagcagca ttttcctaaa ggcaactggc attttttgac tggcaattcc
    11221 gattcaatca ccaaattaac cgagtctgtc ggatttaaat ttaaaaaaga gggtgaagaa
    11281 tttgcacatc cgtcgtcttt aattataata acgcccgacg gaaaaatttc gaggtaccta
    11341 aacggtatcc agcatgaacc aaaggacttg aaaatggcac ttttggaagc gacaaatggt
    11401 gagattggga catctaaaat tattaataaa gcgcttttgt tttgttatca atttgatcca
    11461 gttggaaaga aatatgcact ccaggctcta aatgtagtaa aggcaggagg agttgtaaca
    11521 cttctttcac ttggtgtttt cttaacctat ttttggaaaa gggaaaaaaa gatttctaga
    11581 aaagatggag gttttgacga tagaaatgaa ttgggttcct gacgtagcat cgaattttgc
    11641 aaaccaagtg gatggggttt tatggttcat cacctggtta tcaataatat tttttatact
    11701 tataacaata cttctggttt atttttcttt taaatacaaa agaaaaacag aaaatgatga
    11761 gacacctcat attacaggaa acgaaactct cgaaatcata tggacgatca taccctctgc
    11821 tcttcttatg gttattttcg tatatggatt tgttgtttat aaggatatga gaacacctcc
    11881 gcccgaggct gttgaagtaa acgtgactgc caaacagtgg ctctgggtgt ttaaatatcc
    11941 aaatggccaa agtactatta acgaacttta cgtagaacat aacagacctg taaaaatggt
    12001 gatgagggca gatgatgtgc ttcacagttt ttttgtgcct gcattcagag taaaacagga
    12061 tgtggttccg ggaatgtata cacagctttg gtttaagccc acaaaagtcg gaacatatga
    12121 tctgttctgc acagagtact gtgggaagga tcattccaat atgcttgcca aggtctatgt
    12181 aatgagtccg gaagcatttg cccgttggga aagaggtggt gacgaggaag aggggatagg
    12241 cgcagtagta gctaaatcac ctgaagagct tggtcaaaaa ttatatgaaa aaaatgggtg
    12301 tgctgcttgt catactttag atggcagtaa tagtgtagga cctacattta aagggctatt
    12361 tggcaagact gaaccactac aagacggaac atcagtcctg gctgatgaga actatttgag
    12421 agaatcaatt cttcttccac aggaaaaaat agttgccggt tatgctccgg ttatgccttc
    12481 atttaagggg cttttaaaag acgaagaagt agatgctatt atagcttata ttaaaacact
    12541 gaaataacta acagtatttt ttaggagggg tttttattat ggcaaacggt actgccgcca
    12601 caatgagtga gccatcattt ttagaataca agacggggtt tatgtcttgg gcctggacaa
    12661 ctgaccacaa aagaataggt atcttatacc ttatttccgt aacatttttc tttcttgttg
    12721 caggtgcaat cgcagttatt atgagaaccg agctctttac cccagagtcc gatctcgttt
    12781 caccacacac atataatgtg ctgttcacgc ttcatgggtc gcttatggta tttttcttca
    12841 ttgtgcccgg tgttgcggca agtcttggaa actttcttat tccgctaatg ataggcgctc
    12901 ccgatgttgc ttttccaaag cttaatatgg caagctacta tgtatacata gcgggtacta
    12961 tcataacgtt aattgcgctg cttcagcccg cagacaccgg atggactttt tatacccctt
    13021 atagtgctaa gacgactact aatgtaatct tgataacgtt tggtgtgttt gtcctgggat
    13081 tttcttctat tcttactgga atgaacttta ttgtgacaat tcacaagatg agagccccgg
    13141 gtatgacatg gcacagactt ccgcttttta tttgggcttc ttatgcaaca gcaatcctgc
    13201 agcttcttgc tacaccggtt gttggtatca cgctactctt gcttattgca gaaaggacac
    13261 tcggagttgg atttttcgat ccggataaag gaggagaccc gattctgttt caaaactttt
    13321 tctggttcta ttctcatcct gctgtatata tcatgatcat tccggctatg ggtgtaatct
    13381 cagaaatact tccggttttc tcaagaaagc caatattcgg ctataaggct atagcatatt
    13441 ccagtgtggc gattgctctt attagttttt tagtgtgggc gcaccatatg tttgtaagtg
    13501 gtatttcaga aactgcagca accatattct cgttccttac gttccttgtg gctattccta
    13561 ctgctattaa ggtgtttaac tggattgcta ctctttataa gggatcgata gagtttaatt
    13621 ctgcgatgct ttatgcgctt tcttttatat tcctgtttac aatcgggggt cttacgggac
    13681 ttcacctcgg agcacttgct tcggatattc atctccatga cacttatttt gtagtagcac
    13741 acatgcacta tgtaatgatt ggaggaactg ttataggatt ttttggggcg cttcatttct
    13801 ggtatccgaa gtggtttgga agaatgcttg atgaaacact tggaaagatt gcatggtttt
    13861 tagtatttat aggatttaac gtaacgttct tccctcagtt tttcttaggt gtgcagggaa
    13921 tgccaagaag atatgcaagt tatctgcctg agtttgagcc tctaatgaag ctctctacaa
    13981 taggggcatg gatacttgca attggtattg gtattatggt ttataacatg attaaatcac
    14041 tttattccgg tgaaaaagca agtgataatc catacggctc attatcactg gagtggcaga
    14101 cttcatcacc acctattcac gagaattttc atgaggttcc ggtagttaca gactggacat
    14161 atggatatgg taaaaaagaa ggataaacac aggaggagtt aatttagaaa tgtcagaaat
    14221 tacagctggt cacggtcatg gtcatattca aatagaccct gtagaagaat ataaacagtc
    14281 caaatttggt atgtggctgt tcctgggaac ggaaatcctg ctttttggcg ggctttttgc
    14341 cgcatatgca atttttagag caaagtatcc cgagatgttt catgagcagc atcaacagtt
    14401 agactggcag cttggggcat taaacacagt gatacttctt tttagcagta tgacaatggc
    14461 tattggtgtt actgccagta agcgcggtaa taataaactg cttattgcaa tgctggctat
    14521 aactataatc tgtggactgg cattcggata taataaatat atagaatatg gctataagtt
    14581 ttctcaccat atctaccctg ataagagtat cttctattca ctttacttct taatgaccgg
    14641 gcttcatatg atccatgtat ttattgggct tgtgatattg cttgtagtaa tggtatatgc
    14701 cataagggga gtatattctg cagagtacaa cacacatgtt gaagtaagcg gtctgtattg
    14761 gcacttagtg gatttgatct ggatttactt atttccgtta ctttacttga taggctgacc
    14821 aggaggaaaa tttaaatgag cgaacatcat gaagaacata taacaccaat aaaaacatat
    14881 tttattatat ttgggatttt aatagcttta acagttatta cagtttatac tgcaaaagag
    14941 gttgaccttg gagcatttaa cttcttttta gcaatgatta ttgcatcagc aaaagctaca
    15001 attgtagctc tatattttat gcaccttaag tttgaagaca gtattacatg ggtttttgct
    15061 atttatcccc tctttttgct ggggcttttg ataggactta cctcagtaga catatttaac
    15121 aggataacgc cttaataggg tacacttatt gctgaatgct agaaaaaagt accgttgggc
    15181 tgtttttcct tctattaatc tggggtagtg tggtaacagg cctcgaagcg ggtcttgcct
    15241 gtcctgactg gcctaaatgt catggcgagt acattcctcc tctccgctgg gatatatatg
    15301 tagaattctc acacagggta ttgggtggtg tcgcagggtt acttctaata atacttgcat
    15361 ataaacgatt taaggtttat gaccaggctt atagagccat tccaataatt gcagttgtcc
    15421 ttttagctat acaggttgtt atgggagggt tggttgttct tttaaagctc ccaatgaacc
    15481 ttacaacact gcatttctca actgcactaa taatattttc gcttgtgcta ttcctggcat
    15541 attttaatga ctctagaaaa ccagattttt ctaataatac atttacttta tttttcttct
    15601 tatcattatt agtgctttca cagggcgctc ttggagctta tgtgcgtcat agcggggcgg
    15661 gacttgcatg tcctgacttt cccaagtgtc ttggattttg gataccccct gagctttcag
    15721 gaatagttct aactcatttt ctgcatagaa ccctggctta cataatattt gcagtattta
    15781 tctttctttt aatctattct agaacaacgg acacattaca gaaatataga aagaagattg
    15841 ctatagtatt tggtttaata atattccaga tacttctcgg ggttggaata attcacacaa
    15901 aactatactt tgctgttaca gcaacacatg tctttgtagc tctattaatt ctttatattc
    15961 ttcttaacac ctggtattcg gaaaacacca agcattcgga aaatgctggg agcactgtat
    16021 aatagtggct actatgagtg gggaagcaac taatattaat acggataaag tctcagcggg
    16081 taagtttgtt acatctacag tagtccttct aaaaccccgc attattatta gcgtagtgtt
    16141 tacgggcttt gccggaatgg tgcttgccaa tagagctatt ccagat
//
LOCUS       DBNGSV010000106         6915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1473172, whole genome shotgun sequence.
ACCESSION   DBNGSV010000106 DBNGSV010000000
VERSION     DBNGSV010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1473172"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="AC159U_03760"
     CDS             complement(<1..566)
                     /locus_tag="AC159U_03760"
                     /inference="COORDINATES: protein motif:HMM:NF045274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336203.1"
                     /translation="MVFLGSLAVRAEELGHFIVQGDINYNDFNNLEALNSYTKAYELD
                     PESFEVLMKLTRACNDVGEDLKEEGSKEAERYFIDAFQYSEKMRELFPEKAETYFYLA
                     ATRGNLALSKGGKDKVKLGRYVESYAKKAIEINPDYAPAYVALGVFYREVANLNWFLK
                     KFANAFFGGLPEGTNEDSLKALIKAEKYL"
     gene            762..3833
                     /locus_tag="AC159U_03765"
     CDS             762..3833
                     /locus_tag="AC159U_03765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HIR3336204.1"
                     /translation="MNLQKLTKKQLIDHINSLNTTIGKLQSELRVSEDSDIPDQVSQL
                     GHILDNSLNEILIFDAETFKFVFTNQSARNNLGYSAKEFYDMTPLDINPELTPESADK
                     LVEPLLSGEKSRLNFETALRRKDGSIYPVYVVLQLSTFNGQKVFVANKIDITEQKKAL
                     DELQKSERKYKALYDDNPSMYFTVSEKGVVISVNKFGAEQLGYKPDELIGKPVLNVFY
                     EEDKEAVKEQFSLCLNKPGKIVNWEFRKIKKDGTVIWVKESARVVEKSSGQIVVLIVC
                     DDITEHKKLVEQATQLGEILDSSLNEIYIFDPGTLNLIFVNEGARKNLGYTLDELTCM
                     NALDIKPKISAEDITALVKPLVNGKTDILVFETEHQRKNGSLYPVEVYLQISQFNGQD
                     VFLANIIDVTERKKAQEALRISEERYRVLHDQSPIGICVIDKDLRIIQTNHRMVEMFE
                     STHDKVIGLDLNKLKDKSFVPIISEAFEGKMGKTDALYSATTSSAYKWLSVSSAPIRN
                     TEGKVIYVICVVEDISEMKKAQEALEASEQQLRLIADGLPVFISYVSKNLRYEYVNKY
                     YETWFDTDIKDILGKHIRDLHGNKTFSLLENYFKKVLSGSEQEVEHSVLHPEMGERFI
                     SAKFIPHESQEEILGFYVLATDITDRKKHEEELLHTQKMDSIGLLAGGLAHDFNNYLT
                     SILGNIALAKIAVPKDNDAYIRLENTEKACTRAKNITQQLLTFSKGGEPVKEPLDLAE
                     LIRETAEFSSRGSTSKCNFDIQPDLWKIEADKGQIAQVINNLVINSIQSMPNGGLITI
                     SAMNKTDSTNGNHILLEILDTGIGIEDNHLNKVFDPYFTTKQSGSGLGLTTVYSIIKK
                     HGGTIDIRSLIGRGTKISITLPAINSMSEKTTPKRINGISKYSGKVLVMDDDDEVRDA
                     LCNILSKLGFKIQQTKDGAQTIQEYQKALNNGNIYDLVILDLTVPGGMGGKETIIKLK
                     EIDPNINALICSGYSNDPVMSEFENFGFKGVIKKPYTIDDLKTTLDSVLLK"
     gene            3949..4641
                     /locus_tag="AC159U_03770"
     CDS             3949..4641
                     /locus_tag="AC159U_03770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3336205.1"
                     /translation="MKYKSRPVIGITTDYKDKYFGIEKTYSASIAKHGGLPVLIPSLS
                     SKRILRELTNSIDGLLIPGSRDMNPKYYNQKPHKKLNPMNHQRTVSEYIILEQTLKNN
                     IPVLGICGGMQFINVYFGGSLYQDIQSLLPDALNHEKGAEHSVNIVSDTNLKKIIRTN
                     RFKVNSYHHQAIKKVSKDLIISARSDDDIVEAFESEDGYITAVQWHPELENNLASARL
                     FNHFIKKSAERI"
     gene            4719..>6915
                     /gene="uvrA"
                     /locus_tag="AC159U_03775"
     CDS             4719..>6915
                     /gene="uvrA"
                     /locus_tag="AC159U_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3336206.1"
                     /translation="MNFIKIKGARQHNLKNIDVDIPRNRISVITGLSGSGKSSLAFDT
                     IFAEGQRRYMESLSSYARQFTENLDKPDVDSIEGLSPAISVDQKTFLRNPRSTVGTIT
                     EIYDYLRVFFARIGKPYCYKCNVEISAQNLDQMLVRALNMDKDKYLFVYSPIVQGRKG
                     EYKKELDDLRHEGFLKVKIDGQLFDLDDEIKIDKQKKHNIDVLIDVVEINNKASEIML
                     TDAISESLKRSGGVVAIESDRDNRLILSENFSCPVCSMNYPEISPRLFSFNSPYGACQ
                     KCQGIGTDTFFDPELVVENWDKSINEGTIIPWKKSKYFLDIMQGVSKHYDFSLDTPLN
                     KYSKKISDIVFYGNPDERIKFRKTNKKSSEQYSDTFSGIIGMLSDWYGETNSIDIKET
                     LSKYITTSVCSECGGARLRKESLSILVANNNIFQLSKLSVSASIKFYEKLKLSGREQL
                     IGKAIVSEILSRLSFLAEVGLNYLSLDRSAPTLSGGEAQRIRLASQVGSNLTGITYVL
                     DEPTIGLHQRDNALLINTLKRLRDKGNTVIVVEHDEEMIVNSDHVIDIGRGAGELGGE
                     VIHSGDKSRLIKNKQSLTGNYISGKLKINVPKKRRKQNGQYILIQNPSENNLKINEIS
                     IPLGLFNCITGVSGSGKSTLINDILYKSLMKTLYKSNIKPGNCKEISGAENIKRIINV
                     DQTPIGRTPRSNPVTYTGIFTEIRQMFSMLPESKLRGYTSSRFSFNVSGG"
BASE COUNT         2491 a         1163 c         1245 g         2016 t
ORIGIN      
        1 agatacttct cggcttttat aagcgctttt aaggaatctt cattagtgcc ttcgggaaga
       61 ccgccaaaaa atgcatttgc aaatttcttc aaaaaccagt tcaggtttgc tacttctcgg
      121 taaaaaacac ccaaagccac ataggcaggg gcatagtcgg ggttgatttc aattgccttt
      181 ttagcataac tttcaacata tcttccaagc ttcaccttat ctttaccacc cttagatagg
      241 gctaaattcc ccctggttgc agctaggtaa aagtaggttt cggctttttc gggaaacaat
      301 tcccgcattt tctcggaata ttgaaatgca tctataaaat acctttcagc ctcctttgag
      361 ccttcctctt ttaaatcttc tcctacatca ttacaggctc tggtaagttt catcaatact
      421 tcaaaactct ctgggtctag ttcataagct ttggtataac tatttaatgc ctctaagtta
      481 ttgaaatcat tataatttat atcaccctgg actataaaat gtcccaattc ctccgctctt
      541 acagccaaag aacctaaaaa taccaaagca ataatcttcg taacaatcaa atatctagta
      601 aattcagaat gtttatagat ggttatatct cattaaactc ataaaaattt atctctgata
      661 ttatgactaa atttttcaac atagttggca tagcgtttgc actgtatata acagggtgct
      721 ttttgtctta aataaattaa actttaattg taatgataga aatgaattta caaaaattaa
      781 ctaaaaaaca gttaattgat catataaata gtcttaacac tactattggt aaactacaat
      841 ctgagcttag agtttcagaa gactcagata ttcctgatca ggtttcacaa ttaggtcata
      901 tacttgataa ctcccttaat gaaatactta tatttgatgc tgagaccttt aaatttgtgt
      961 ttaccaatca atcggcaaga aacaacctgg ggtattctgc taaagaattt tatgacatga
     1021 ctccccttga tattaatcct gaactcacac ctgaatctgc tgacaagctt gttgaacctc
     1081 tactaagtgg tgagaaatcc aggcttaact ttgaaactgc tttaagaaga aaagacggtt
     1141 caatctatcc tgtttatgtt gttctgcaac tttcgacctt taacggtcaa aaagtatttg
     1201 ttgctaataa aattgatatc accgagcaaa aaaaggcttt ggatgagctt cagaaaagtg
     1261 aaagaaaata taaagcttta tatgatgata acccttccat gtattttacg gttagtgaga
     1321 aaggagtagt tatatcagta aataaatttg gcgccgaaca attaggatat aaaccagatg
     1381 aattgatcgg taaacctgta ctaaatgtat tctatgaaga ggataaagaa gctgtaaaag
     1441 aacagttctc actttgttta aataaaccgg ggaaaattgt aaattgggaa tttagaaaaa
     1501 taaaaaaaga cggcacagta atatgggtta aagaaagcgc cagggttgtt gagaaatcat
     1561 caggtcaaat agtagttttg atcgtatgtg atgatattac agaacataaa aaactggtag
     1621 agcaggcaac acagcttgga gaaattctgg actcatcgct taatgaaatc tatattttcg
     1681 atcccggtac actaaatctt atttttgtaa acgagggtgc aagaaaaaat ctgggatata
     1741 cactggatga acttacttgt atgaatgcac ttgatataaa accaaaaata tccgctgaag
     1801 atattactgc actggtaaag cctctagtaa atggaaaaac agatattctg gtttttgaga
     1861 cagaacacca aagaaaaaat ggctctctat atccagtgga agtatatctg caaatctctc
     1921 aatttaatgg acaggatgta tttttagcaa atattataga tgttacggaa agaaaaaaag
     1981 cacaggaagc tttaagaata agtgaagaac gctacagagt actgcatgat caatccccta
     2041 taggtatatg tgtaattgat aaggatctta gaatcattca aaccaatcat agaatggtcg
     2101 agatgttcga gtctactcat gataaagtaa tcggacttga tcttaataaa ttaaaggata
     2161 aaagctttgt tcctattatt tcagaagcgt ttgaaggaaa aatgggcaaa acagatgccc
     2221 tgtatagcgc aactacaagc tctgcatata aatggctttc tgtaagttca gcccctatta
     2281 gaaataccga gggaaaggtc atatatgtta tttgtgttgt agaagatatt tcagaaatga
     2341 aaaaagcaca agaagcatta gaggcaagcg aacagcagct gagattgata gccgatggtc
     2401 ttccagtatt tatttcctac gttagtaaaa acctgcgtta tgagtatgta aataaatact
     2461 atgaaacatg gttcgacacc gatatcaagg atattttagg caagcacatt cgagatcttc
     2521 atggaaacaa aactttcagc ctattggaaa actattttaa aaaagtacta tcaggttctg
     2581 agcaagaagt tgaacattct gttttgcatc ctgagatggg tgaaagattt ataagtgcta
     2641 agttcatccc tcatgaatcc caagaagaga ttttaggttt ttatgtttta gcaactgaca
     2701 taaccgatag aaaaaaacat gaagaagaac tcctgcacac acagaaaatg gattcaattg
     2761 ggcttctggc cggagggctt gcccatgatt tcaataacta tctcacttct atattaggta
     2821 atattgctct tgccaaaatt gcggttccaa aagataacga cgcatatatt agattagaaa
     2881 atactgaaaa agcatgtaca cgggctaaaa acattactca gcagcttcta actttttcaa
     2941 aaggtggtga acctgtaaaa gaacctttgg atttagcaga gttaataaga gaaaccgcag
     3001 agttttcttc aaggggttct acaagtaaat gcaactttga tatacagccc gacctttgga
     3061 aaattgaagc cgataaagga cagattgccc aggtaattaa caatcttgtg atcaattcaa
     3121 tccagtccat gcctaatgga ggattaatta ccatatctgc aatgaataag acagactcta
     3181 caaacggaaa tcacatactc ttagaaatac tagacactgg aataggaatt gaagataatc
     3241 atttgaataa ggtatttgat ccttatttta ctaccaaaca gtccggaagc ggactaggtc
     3301 ttacaacagt ttactccatt attaaaaaac atggtggcac tatagatatc agatctctta
     3361 taggaagagg aaccaaaata tcaattactc ttccggcaat taattctatg tcagaaaaaa
     3421 caactcccaa gagaattaat ggtatttcaa aatattccgg aaaagtttta gtgatggatg
     3481 atgatgatga agtaagagac gccctttgca atatactaag taaacttgga tttaaaattc
     3541 aacagaccaa agatggagct caaaccatcc aggaatatca aaaagcgtta aataatggga
     3601 atatatatga tttagtaatc ctagacctta ctgttcctgg cggaatgggt ggaaaagaaa
     3661 caattatcaa acttaaagaa atagatccta atatcaatgc tctaatatgc agcggttatt
     3721 caaatgatcc tgtaatgtct gagtttgaga actttggatt taagggtgtt attaaaaaac
     3781 cctatacaat agatgatctt aagacgacac tcgactctgt actattgaaa taaatagtta
     3841 gaatttatct agttacagga cctgagtttt ttcaaaataa tctatagtaa taaaaaataa
     3901 caagactata atcttacata ttattatagc tttactgcac cctgtattat gaaatacaaa
     3961 tcaagacctg ttatcggtat taccactgac tataaagata aatacttcgg aattgaaaag
     4021 acttattctg cttcaatagc aaaacacgga ggattaccgg tgcttattcc ttcgcttagt
     4081 agtaaaagaa ttcttagaga acttacaaac agcatagatg gtctattgat acctggttca
     4141 agagatatga atccaaaata ctacaaccaa aagcctcaca aaaaattaaa cccaatgaac
     4201 catcaaagaa cagtttcaga gtatattatt ttagagcaga cacttaaaaa caatattcct
     4261 gttcttggaa tttgcggagg tatgcagttc atcaatgtat atttcggagg ttcattatat
     4321 caggacatac agtctctttt gccagatgca ttaaaccatg agaaaggagc agagcactca
     4381 gttaacatag taagcgatac aaacttaaaa aagataatta gaacaaacag atttaaagta
     4441 aacagctacc accatcaagc aataaagaaa gtcagtaaag atttaattat ttctgcaaga
     4501 tcagatgatg atattgtaga agcttttgaa tctgaggacg gatatataac tgctgtccag
     4561 tggcatcctg aacttgaaaa caatctggcg tcagcacggc tattcaatca ttttataaaa
     4621 aagtctgctg agagaatcta aattagttat atcaaccata atactatttt atatactttt
     4681 attatttgtt catttttttt aggtaaactc ccattacttt gaatttcatt aagataaagg
     4741 gtgcccggca gcacaatcta aaaaatatag atgtagatat tccaagaaac agaatttctg
     4801 taatcacagg tcttagtggt tccggcaagt cttctcttgc ttttgatact atttttgcag
     4861 aaggacaaag aagatatatg gaatcacttt cttcgtacgc cagacaattt actgaaaacc
     4921 ttgataaacc agatgttgat tcgattgaag gtctttctcc tgcaatctca gtggatcaga
     4981 aaactttttt aagaaatccc cgatcaacag ttggaactat tacagaaatc tacgattact
     5041 taagggtatt ttttgcaaga ataggtaaac catactgcta caaatgcaat gtggaaatca
     5101 gtgcccagaa tctggatcag atgttagtaa gggcattaaa tatggataaa gataaatatt
     5161 tgtttgttta ttctcccata gtacaaggca gaaaagggga atataagaaa gagcttgatg
     5221 acctgagaca tgagggattt ttaaaggtta agatcgatgg acagcttttt gatcttgatg
     5281 atgagataaa aatagacaag caaaaaaaac ataatataga tgttctaata gatgtagtgg
     5341 aaattaacaa taaagcatct gagataatgt taaccgatgc aatttcggaa tctcttaaga
     5401 gatcaggtgg tgttgtagcc attgaatcgg acagagataa cagacttata ctgagcgaga
     5461 atttttcatg tcctgtatgc agtatgaact acccggaaat ctctcccaga cttttttcat
     5521 ttaacagtcc gtacggtgct tgccaaaagt gccagggaat aggcaccgac acgttctttg
     5581 atcctgaact cgtggtagaa aactgggata aatctattaa tgaaggaact ataataccat
     5641 ggaaaaaatc caaatatttc cttgatatca tgcaaggcgt atcaaaacac tatgatttca
     5701 gtctggatac acctctaaac aaatactcaa aaaaaatctc agacatagtc ttttatggaa
     5761 atccggatga gaggataaaa tttcgaaaaa ccaataaaaa atcaagtgag caatattctg
     5821 atacattttc cgggattatt ggaatgctct cagactggta tggcgaaaca aattcaattg
     5881 atataaaaga gactctttca aaatatataa caacttctgt ttgcagtgaa tgtggtggag
     5941 ccaggcttag aaaagaaagt ctttctatcc ttgtagcaaa taataatatt tttcaacttt
     6001 caaagctatc agtttcagcc tctataaagt tttatgaaaa attaaaatta agcggcagag
     6061 aacagcttat tggaaaagca atagttagtg aaattctaag caggctcagt ttccttgctg
     6121 aagtgggttt aaattaccta tcgctcgata gatccgcccc gacactttct ggcggtgaag
     6181 cacagagaat aaggcttgca agccaggtag gttctaacct tactgggatc acatatgtac
     6241 ttgacgaacc cacaattggt cttcaccaaa gagacaacgc cctgttaata aacactctta
     6301 aaagactgcg tgataaagga aacaccgtaa ttgtagttga gcatgatgaa gaaatgatag
     6361 taaattctga tcatgtgatt gatattggca ggggagcagg ggagcttgga ggcgaagtaa
     6421 tacacagtgg agacaaatct agattaataa aaaacaaaca atctcttact gggaattata
     6481 tttccggaaa gctcaaaata aatgttccta aaaaaagaag aaaacaaaac ggacagtata
     6541 tacttatcca aaacccctca gagaacaatc tgaagataaa cgaaatttca ataccacttg
     6601 gtttatttaa ctgtatcacc ggtgtttcag gttctggcaa gagcacgctt ataaacgata
     6661 ttttgtataa atcactaatg aaaacgctat ataagagtaa tattaagcca ggaaactgta
     6721 aggaaatttc aggtgctgaa aacataaaaa gaattattaa tgtagatcaa acaccaatag
     6781 gcagaactcc caggtcaaac cctgttacgt acactgggat ttttacagaa attaggcaga
     6841 tgttttcaat gcttccggaa tcaaagctaa gaggatacac atcttctaga tttagtttta
     6901 acgtaagcgg aggga
//
LOCUS       DBNGSV010000107         1741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_989480, whole genome shotgun sequence.
ACCESSION   DBNGSV010000107 DBNGSV010000000
VERSION     DBNGSV010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_989480"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="AC159U_03780"
     CDS             <1..1046
                     /locus_tag="AC159U_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3336207.1"
                     /translation="SNDPDWKIQTVKKCMRRKFDKPDLIIAHIYPPQSLAQYAQKDVP
                     LVSYIAWEVDMLASSWAYHLNLYSDYIITTCFEMKKVFIKSGVTKPIHVLGPTVFQED
                     VDKIDRSPVSKEQLPPGLTAFYRPDKFIFYSVFQWIERKNPNILVEAFLQEFDNTEDD
                     VCLLLKTYRADYSEGEMRAIHSDINLIHHELNLLYAPEIRIVTHMLTNEEMELLHRIG
                     TCYISPHRGEGTGLGIMDAILYHNPVITTPYGGPSDFTDPSSSRWLDYKMIPIRNKAW
                     CMNYFNSSMNWAAVDIFDLRTAMREAYLDWKFNKRERSKVLTPMAEAKLMEIYSYEAN
                     SKGMSEILESCSK"
     gene            1043..1714
                     /locus_tag="AC159U_03785"
     CDS             1043..1714
                     /locus_tag="AC159U_03785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336208.1"
                     /translation="MNFNLKNIKDNIDDIDSYEVLAYITREFDIKSYLEIGTREGCSA
                     ATVVCNDDKEARTYFRDVTWAGSSFTEEMLKHTEKFTPVYTGTMTLCDTWGLQYGGTG
                     RNDSRVVSDLLLNRIGYNKDLLTIITGDSKTEIPKLTDTYDLIYVDGDHSYHGARADL
                     INVKDKANKFIVFDDIYHPDHKELLHCFNEFIECHMPKDKITVSICGKNNVGVAIIMK
                     EEMDR"
BASE COUNT          590 a          276 c          362 g          513 t
ORIGIN      
        1 actcaaatga cccagactgg aagattcaga ctgtcaaaaa gtgcatgaga cgcaaatttg
       61 acaagcctga tcttataatc gctcacatat acccccctca atctttagca caatacgcac
      121 aaaaagacgt gccccttgtt tcatatatcg catgggaagt agatatgttg gcgagttcct
      181 gggcatatca tcttaatcta tattcagatt atataatcac tacatgcttt gagatgaaga
      241 aagtattcat caaatcaggc gtcaccaaac ctatacatgt tctaggccct actgtatttc
      301 aggaagatgt ggataaaata gacagaagtc ccgtgtctaa agaacaacta cctcctggtc
      361 ttactgcttt ttataggcct gataagttca ttttctatag tgtattccaa tggatagaac
      421 gcaagaatcc caatatatta gtggaggcat ttctacagga gttcgataat acagaagatg
      481 atgtatgttt gctgttgaaa acgtatagag ctgactattc agaaggagaa atgagggcta
      541 ttcattccga tataaaccta atccaccatg aacttaactt attgtacgct ccggagatac
      601 gaatagtgac tcatatgtta actaatgaag aaatggagtt gctgcataga attgggacat
      661 gttatataag cccacatcgt ggagaaggta ccggtttagg tattatggat gctattctat
      721 atcataatcc agtaataact acaccctatg gcggtccgtc agatttcaca gatccatcat
      781 catctcgttg gttagattat aaaatgatac caatacgcaa taaagcatgg tgcatgaatt
      841 acttcaattc tagtatgaat tgggctgctg ttgatatatt cgatcttaga accgctatgc
      901 gggaggctta tcttgactgg aagtttaaca aaagagaacg ttctaaagta ttaacgccga
      961 tggcagaagc taaactaatg gagatatatt cctatgaagc taattccaaa ggaatgagtg
     1021 aaatattgga gagttgtagt aaatgaattt caatcttaag aatataaaag ataatattga
     1081 tgatatagat agttacgaag tacttgcata tattacaaga gaatttgaca ttaaatcata
     1141 tctggaaata ggtactagag aaggatgttc tgctgctact gtagtgtgca atgatgataa
     1201 agaagctagg acttatttta gagatgttac atgggctggc agttctttta ctgaagaaat
     1261 gctgaagcat actgagaagt ttactcctgt gtatacaggg acaatgactt tatgtgatac
     1321 atggggatta cagtatggcg gcaccggtag aaatgatagt cgtgtagtta gtgatttatt
     1381 actaaataga atagggtata ataaagatct attgactatt attactggcg attctaaaac
     1441 agagatacct aagttaactg atacatatga tctaatatac gtggacggag accatagcta
     1501 tcatggagcc agggctgatc ttataaatgt caaggataag gcaaataaat tcatagtgtt
     1561 tgatgatata taccatccag atcacaagga attactacat tgcttcaatg aattcattga
     1621 atgtcatatg cccaaggata aaataactgt tagtatttgc ggtaagaata atgtaggagt
     1681 tgctattatt atgaaggaag agatggacag gtagtgtatg gagaaaggta tgaccgcctt
     1741 a
//
LOCUS       DBNGSV010000108         6041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1335218, whole genome shotgun sequence.
ACCESSION   DBNGSV010000108 DBNGSV010000000
VERSION     DBNGSV010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1335218"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC159U_03790"
     CDS             <1..249
                     /locus_tag="AC159U_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIR3336209.1"
                     /translation="PQAPTHILVIPKKHIRSLVESDESDTLLMGHLTKVARNIANEKG
                     LDEKGFRLVLNSGLQAGQSVWHVHFHLLGGRKMNWPPG"
     gene            280..1560
                     /locus_tag="AC159U_03795"
     CDS             280..1560
                     /locus_tag="AC159U_03795"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3336210.1"
                     /translation="MQQNEQLAYLNSLGLHNIKPGLQRITNVLELLGNPQNTFKSIII
                     GGTNGKGSVSAAVASVLIQSDIKTGLYTSPHLIKVNERISINNSTISDSDLEGLIKYL
                     RIILEKHNIRLSYFEFITTLSFIYFSVFKIDIAVLEVGMGGRWDATNVVTPLVSVITN
                     ISFDHMEYLGSTLSEIAQEKAEIIKNNIPVVTACSNKSIEPIIRKAYSCSAPLYIYGT
                     EFNTDGSSTDNFSYKSRSSFLDNLSSNLSGKFQVENICVALKTLDILINKYKYNISEA
                     SIRKGLQKINWPGRFEILDEKRPVILDCAHNEESSNVLIHSLKQLYPDTSFDFLIGML
                     DDKQHTIFIKNIAAISNTIIITNGFSQKEADTGELSEICKELNCNFETIDNYKTAFNR
                     LINDTSPLCITGSIYLVGAIKKLLIETDSNFHKI"
     gene            1566..2597
                     /locus_tag="AC159U_03800"
     CDS             1566..2597
                     /locus_tag="AC159U_03800"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3336211.1"
                     /translation="MRKVGLVIDELFVKHINSFDHPESPDRVYVINDMLNETGLINHL
                     TFLEPRDAKKEDITRVHTEKYFQIIEKTKGKEKVFLDQDTSTNAFSFDSAMRASGGVL
                     QSIDKIIKNDIDTSFCIERPPGHHAEPDRSMGFCLFNHAAVGAGYLRSKGFERILIID
                     WDVHHGNGTQHIFESSKEVLFFSTHQFPFYPGTGSLEELGIDDGAGYTVNVPIEIGMG
                     DSEYIKIFNELLIPITSQFNPQFIIVSAGFDSFIEDPLGGMNVSTEGFSKLTEIVINL
                     SEQCCEGNLLLILEGGYNLQGLADCTQKVFDTLINYDGNRDHKITEPTKADNTIDIVK
                     NNYSEFWDF"
     gene            complement(2594..3421)
                     /locus_tag="AC159U_03805"
     CDS             complement(2594..3421)
                     /locus_tag="AC159U_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3336212.1"
                     /translation="MPRTSKTKKKKQYGCEQCFGNGYELKPEGKYSVAQVCSCLKCDQ
                     CSGTGKKQYYKDSGYSYLKPCEQCGYLHKNVKLYNLAAIPSKYYSVSEVSTFNPRHDT
                     HQDALIYVKQFVDQYPHHKGFLLMGASGLGKTHLAICAISELTLEKGVKCLFVDFFDL
                     LNDLKSAYSEGRPENQVINPLIDTDVLVIDELGKGRANEWELGVLDQLISKRYNSTKI
                     TVITTNFVTKDINVNLNRDSKEILEYRVGERISSRLFDMCSFFYLEGRDFRRMNKKN"
     gene            complement(3423..4067)
                     /locus_tag="AC159U_03810"
     CDS             complement(3423..4067)
                     /locus_tag="AC159U_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336213.1"
                     /translation="MFKEDSYIKSIEKYFLNHLGKGIMISYSDYELIQRWKDRQIPYE
                     VIIEGISNAFSDSKNFDSLNNKTKIRNLGYIISYIDKSIEIYSGLKHIDISNDEKSTN
                     SYVDKIISKIGAGINHLDDDNLISTINSYKENIASLKCDDHTQIYALVREKELELYNR
                     IFNLLSQLEKNTIIDKAKSNIKNRDNMTQEAYDKSITSFRNMLIKKRFNLEFLN"
     gene            complement(4097..4777)
                     /gene="rpe"
                     /locus_tag="AC159U_03815"
     CDS             complement(4097..4777)
                     /gene="rpe"
                     /locus_tag="AC159U_03815"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3336214.1"
                     /translation="MSKDNKKLIAPSILAADLVRLGEEITAVKQAGVDWIHVDVMDGH
                     YVPNISLGIPEVMSIAKIDPPPMDIHLMIDNPDDFVEVFIDAGAPHVKLVTVQIEATK
                     LLHSTIQKIKNKGVMAGIAVNPTTPISALEEVLELVDLILVMTVEPGFAGQKFIPSML
                     PKIKKLRNIIDKLDNKPLIEVDGGIKLNNIEEIANYGPDVFVSGSGIFASDDYAQTIS
                     DMRELIDN"
     gene            complement(4779..>6041)
                     /gene="rsmB"
                     /locus_tag="AC159U_03820"
     CDS             complement(4779..>6041)
                     /gene="rsmB"
                     /locus_tag="AC159U_03820"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR3336215.1"
                     /translation="IVLNQYLSKNKNIISKDIKLITELVYGTIRWKKKLSYVLNLFIK
                     HKLKSSKIELVLLMGIYQILYLDSVPDHASINETVKVAKAKFDYKIKNLVNAVLREVI
                     RNKELIEYPDKSDISEYLSINYSYPKWLIIKWTESYGEELTEKICAGLNNHGLPIIRV
                     NNNLISTDKFAKLIKDHNPQNTFFSDHGLYVENLHVLINSDLYNNGYFSIQDESSQLV
                     SIILDPKPGETIFDSCSAPGTKSNHIAEIMKNRGKIISCDINKERLNLVLQDSKRLKN
                     SIIFTRCSDAKDIVFDDNIQFDRILVDAPCSGLGTIKNNPDIKWNKNPDGIVQLSSIQ
                     LEILNNVSRYLKTGGTLVYSVCTLMREENEHVCELFLKQNTKFRIDSTVSNKYEKINR
                     LLDRNGYFSSLNELDKMDGFFIAKFQKI"
BASE COUNT         1988 a         1026 c          916 g         2111 t
ORIGIN      
        1 ccacaggcac caactcacat attagttata cccaaaaagc acatcagatc acttgtagaa
       61 agtgatgaat cagacactct acttatggga catcttacaa aggttgcaag aaatattgca
      121 aacgaaaaag gattagatga gaaaggattc agactagtct taaattcagg tctgcaagct
      181 ggtcagtccg tatggcacgt acactttcat ctcttaggag gaagaaagat gaactggcca
      241 cctggataac ggtctattga ttcatattat attaactaca tgcaacaaaa tgaacaatta
      301 gcttatttaa actctctggg tctgcacaat attaaacctg gattacaaag aataactaat
      361 gttctagaac ttttgggaaa cccccaaaat acatttaagt caataattat aggcggaacc
      421 aacggtaaag gttcagtatc agcagcagtt gcatccgtac tgatacaaag cgatattaag
      481 acaggtcttt atacttctcc tcatttaata aaagttaatg aaagaatatc aataaacaat
      541 tctactatta gtgactctga ccttgaaggt cttatcaagt atcttaggat catattagag
      601 aaacataata tcagacttag ttattttgaa tttattacta cactttcttt tatatatttc
      661 agcgtattta aaattgatat tgccgtatta gaagttggca tgggtggaag gtgggatgct
      721 acaaatgtag taacacccct tgtgtctgtt attacaaata tatcctttga tcacatggaa
      781 tatttgggca gtactctaag cgaaatcgct caagaaaaag cagagataat taaaaataac
      841 ataccggtcg taactgcatg cagtaataaa tcaatagaac caataatcag aaaagcatat
      901 tcatgctctg caccattata tatatatgga accgaattta atactgatgg aagctctacg
      961 gataattttt cttataaaag cagatcatca tttcttgata acctaagttc aaatcttagt
     1021 ggaaagtttc aagtagaaaa tatatgcgtt gctttaaaaa cattagatat attaattaat
     1081 aagtataaat ataatatatc tgaagcatcc atcagaaaag gattacaaaa aattaactgg
     1141 ccgggtagat ttgaaattct agatgagaaa agacctgtta ttttggactg tgctcataat
     1201 gaagaatctt caaatgtatt aattcattct cttaaacaac tctatccaga tacttctttt
     1261 gattttttaa ttggaatgtt agatgataaa caacacacta tcttcattaa aaatattgca
     1321 gccatatcta atacaattat tatcacaaat ggattttctc aaaaagaagc agatactggt
     1381 gaattgagtg aaatatgtaa agaacttaac tgtaatttcg aaactattga taattacaag
     1441 actgctttta atagacttat taatgatacg tcccctcttt gtattacagg ttcaatttat
     1501 ttagtaggag ctattaaaaa gttattgatt gaaactgatt caaacttcca taaaatataa
     1561 actaaatgag aaaagtcgga ctagtaatag acgaattatt tgtaaaacat ataaactcct
     1621 tcgaccatcc ggaatctccc gacagggtgt atgttataaa tgacatgtta aacgaaacag
     1681 gattaataaa tcatttaact ttcttagagc caagagatgc aaagaaagaa gatattacaa
     1741 gagttcacac agaaaaatat tttcaaataa tagaaaaaac aaaaggcaag gaaaaagtat
     1801 ttctcgatca ggatacatct acaaatgctt tttcattcga ttcagcaatg cgcgcctcag
     1861 gtggagtatt acagtcaatc gataaaataa ttaaaaatga catagatact tcattttgta
     1921 tagagcgtcc ccctggacat catgcagagc ccgatagatc tatggggttc tgtcttttta
     1981 atcatgcagc agtaggtgca ggatatttaa gatctaaagg ctttgagaga atcttgatta
     2041 tagactggga tgttcaccat ggtaacggca ctcaacatat atttgaatca tctaaagagg
     2101 tacttttctt ttctactcat cagtttccgt tttaccccgg tacgggttct cttgaagaat
     2161 taggaataga tgatggtgct ggttatactg taaatgtgcc aattgaaatt ggtatgggtg
     2221 atagtgaata cattaaaata ttcaacgaac ttttaatacc aataacttcg caatttaacc
     2281 ctcagtttat tattgtttca gctggttttg attcttttat tgaagaccct ttaggcggca
     2341 tgaatgtctc aaccgagggg ttttcaaaac tcacagaaat agttattaat ctttcagagc
     2401 agtgttgtga aggaaactta ttattaatat tagaaggtgg ttataatcta caaggtcttg
     2461 cagattgtac acaaaaggta tttgatactt taataaatta tgatggcaat agggatcata
     2521 aaataacaga acccacaaaa gctgataata ctatagatat tgttaaaaat aattattcag
     2581 aattctggga tttttaattt tttttattca ttcttctaaa atccctgcct tctaagtaga
     2641 aaaaactgca catatcaaag agtctagacg atattcgctc ccctactctg tattctagga
     2701 tttctttact atctctattt aaattcacat ttatatcttt tgttacaaag tttgtagtaa
     2761 taacagtaat ctttgtagaa ttgtatcttt tggatataag ctgatccagt actcctagct
     2821 cccattcgtt agcccttccc ttaccaagtt catctattac gagtacgtcc gtgtctatca
     2881 gtggatttat aacctgattt tccggtctac cctctgaata tgctgatttt aagtcgttta
     2941 aaagatcaaa aaagtctaca aaaagacact ttacgccttt ttccagtgta agctcagaaa
     3001 tagcacatat agccaaatgt gttttaccaa gcccagaggc tcccattagc agaaatcctt
     3061 tatgatgagg atactgatca acgaattgtt tcacataaat aagagcatcc tgatgggtgt
     3121 catgtcttgg attaaaagtg ctaacttctg acactgaata atattttgat ggtatagcgg
     3181 ccagattata cagtttaaca tttttatgaa ggtaaccgca ctgctcacat ggttttaaat
     3241 agctataccc actatcttta taatactgtt tcttgcctgt accggagcat tggtcacatt
     3301 taaggcagct acatacctgt gcaactgaat acttaccttc tggctttagc tcataaccat
     3361 ttccaaaaca ttgttcgcat ccatactgct tttttttctt ggtctttgaa gtccttggca
     3421 tatcagttta aaaattctaa gttaaacctt ttttttatca acatatttct aaaagaagta
     3481 atacttttat cataagcctc ttgagtcatg ttatcacgat tcttaatatt gctctttgct
     3541 ttatcaataa ttgtattttt ttctaattgt gaaagcaaat taaatattct gttatataat
     3601 tctaattctt tttcccttac cagtgcatat atttgagtat gatcatcaca ttttaaactc
     3661 gctatgttct ctttataaga atttattgta gatatgagat tatcatcatc taaatgattg
     3721 attccggcac ctattttaga aattatttta tcaacataac tattggtact tttttcatca
     3781 ttagagatat caatatgttt tagtccggaa tatatttcta ttgacttatc tatgtagctt
     3841 ataatataac caagatttct tatctttgtt ttattgttta gactgtcgaa gtttttagaa
     3901 tctgaaaagg cattactaat cccttctata ataacttcat acggtatttg tctgtccttc
     3961 catctttgta tgagctcata atccgagtat gaaatcatta ttccctttcc aagatggttt
     4021 aaaaagtatt tctcaatact ctttatatag gagtcttctt tgaacattta ttatagatca
     4081 aagatattat atataattaa ttatcgatta actctctcat atctgagatg gtctgagcgt
     4141 aatcatctga tgcaaaaata cccgagcccg aaacaaatac atcgggaccg taatttgcaa
     4201 tctcttcaat attgttgagc tttattccac catcaacctc aataagaggt ttattatcaa
     4261 gtttatcaat tatatttctt aattttttaa tttttggcaa cattgagggt atgaactttt
     4321 gtccggcaaa tccaggctcc actgtcataa caagtatgag gtctacaagc tccagcactt
     4381 cttccaatgc tgaaattgga gtagtaggat ttacagctat cccagccata actcccttat
     4441 tcttaatctt ttgaatagta ctgtgcagta gtttagtagc ctcaatttga actgtaacca
     4501 gttttacatg gggtgcgcct gcatcaatga atacctctac gaaatcatca gggttatcta
     4561 tcataagatg aatatccatt ggaggaggat caatttttgc aattgacata acctcaggta
     4621 ttccaaggct tatatttggc acataatgac cgtccatcac atcaacatgt atccagtcaa
     4681 ctcctgcctg cttaacagcg gttatttctt caccaagtct tacaaggtcg gcagccaata
     4741 tggaaggggc tataagtttt ttattatctt tgctcatttt atattttttg aaatttagct
     4801 ataaagaaac catccatttt atctaactca ttaagcgaac taaaataacc atttctatct
     4861 aaaagtctat ttattttttc gtatttatta ctcacagtac tgtcaatcct aaatttagtg
     4921 ttttgtttta aaaataattc acatacgtgt tcattttcct ctctcattaa agtacatact
     4981 gaataaacca acgttccacc tgtcttaaga tacctggata cattgtttaa tatctctaac
     5041 tgtatggacg ataattgtac aataccatct ggatttttat tccattttat atccggatta
     5101 ttttttattg taccaagtcc tgagcatggg gcatcaacca gtattcggtc aaattgaata
     5161 ttatcatcaa agactatatc ttttgcatct gaacatctgg taaagataat gctattttta
     5221 agcctctttg aatcttgtag aactaaattc agcctttcct tgttgatatc gcatgaaatt
     5281 atttttcctc gatttttcat aatttcagca atatggtttg attttgtacc gggagcgcta
     5341 catgaatcaa aaattgtttc acctggttta ggatctaaaa ttatagatac aagctgtgat
     5401 gattcatcct gaatggaaaa ataaccatta ttatacaaat cagaatttat gagtacatgt
     5461 aaattttcaa catataaacc gtgatccgaa aaaaaggtat tttgagggtt atgatcttta
     5521 ataagttttg cgaatttatc tgtcgatata aggttattgt ttacccgtat aattggtagg
     5581 ccatgattat ttaatcctgc gcagatcttt tccgttaatt cttcaccata agattcggtc
     5641 catttaatta tcagccattt aggatatgaa tagttaattg agaggtattc ggatatatct
     5701 gatttatcgg gatactctat caattcttta tttctgatta cttctctaag aacagcattt
     5761 accaaatttt ttatcttata atcaaacttt gccttagcca ctttaactgt ctcattaatt
     5821 gatgcatgat ccggtacaga atccaaatac agaatctgat aaatacccat aagtaataca
     5881 agctcaattt tactgctttt aagcttatgt tttataaata aatttagtac ataacttaac
     5941 ttttttttcc atcttatagt tccgtaaact aattcagtaa taagtttaat atcttttgat
     6001 attatatttt tatttttaga taaatactga tttaagacaa t
//
LOCUS       DBNGSV010000109         4941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1197121, whole genome shotgun sequence.
ACCESSION   DBNGSV010000109 DBNGSV010000000
VERSION     DBNGSV010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1197121"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..441
                     /locus_tag="AC159U_03825"
     CDS             103..441
                     /locus_tag="AC159U_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HIR3336216.1"
                     /translation="MLSYASSLNNNELNLFTDEFRSPIFGDDLAMAILELADTEYRGL
                     INIAGPKAINRYDLGCRIAKYFGLKTDLLRPVEAKELNLNRPLNCTLESSKATNILST
                     LIKPISKTFT"
     gene            complement(434..1105)
                     /locus_tag="AC159U_03830"
     CDS             complement(434..1105)
                     /locus_tag="AC159U_03830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3336217.1"
                     /translation="MPQKIALYHNTKLIEQTFGLRTGSIRHEPRYNVNPGQNIPIITN
                     NNETECIIMRWGLVPGWSNDPLIGFQLINAKLETINQDKNFKNSLRNKRCIVPCSGYY
                     VCKRIDRTTKVGYFIRHKNEDIFGIAGLWDIWGRDGGNLTTFTLVTTTSNDLINPISD
                     RMPVILNKEDEQKWLDSNIQDPAEVIPLLRTYPSEQMEVYEISNYVNRAENEGSECIE
                     PINVK"
     gene            complement(1124..1798)
                     /locus_tag="AC159U_03835"
     CDS             complement(1124..1798)
                     /locus_tag="AC159U_03835"
                     /inference="COORDINATES: protein motif:HMM:NF040120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HIR3336218.1"
                     /translation="MNSSKILQDNLIEELRKHFHIDSSAVFLLISNLITIFIAVYEKW
                     NLSELMWIYWGQSVIIGFFNRKRILDLKKFSTTGFQLNNKPTKPTKKTQKTAANFFAM
                     HYGIFHLVYLIFIFDSVDHLNKDSLIYILVCISVFLSNHLYSYIHNRERDMNRVPNIG
                     TVMIFPYLRIVPMHIAIIFGGLFAKGSVIALVLFLLLKTFADLVMHMIEHAQARQKSN
                     TFLERN"
     gene            1911..3368
                     /locus_tag="AC159U_03840"
     CDS             1911..3368
                     /locus_tag="AC159U_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3336219.1"
                     /translation="MMKKLIFSVFLILIFIGCDEEQVDGNRPPMINRIDISHSLESGI
                     NRYVVNTDATDPDNDNITYSFDTSSNITILASTSTALINIPQSTGTPPDIIIKVIAED
                     DKGGIATREFNLRTGNKAPVILFYEADKYKLSKGENTKLHALVGDDDGSGDTYSTTFR
                     ISSAITSGILNVTTNNQSLPRASYTGSQSNSENATIELEAIDTDNNLKASSFLTLEVG
                     TGLVHVDSWDTSPIQPYGLDFSSNGHLFITSIGSTVMELDTNGNKVNGNGWNVTDPSS
                     THLRALTVDKRTQNEVLFLGSDRGTHEFIIKYSKTGSAQTDNKTTFPGIPHYFYIRPN
                     GTILISNNKPSSYGINILNTNGTIQSWTSAGQLSQPRQISLDPNTNNVYVADRGNGRV
                     RVFDSSGSFIRNITGNSGQFSNPHGVLADRVHNSVFVADTENDRIQEFAFDGTFKAEF
                     RSNDVNKPRYIIRGPDNRIYVASYIDKEIHVFEHR"
     gene            3380..4171
                     /locus_tag="AC159U_03845"
     CDS             3380..4171
                     /locus_tag="AC159U_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3336220.1"
                     /translation="MKTGFLQFAPKLNDSGFNIQKIESFAEQFKDADLLVLPELCNSG
                     YNFKSKKQAFDTSEEIENSEFINFLESICIENSLFIVSGFNERDGGDLYNSSVLIGPD
                     GFIGNYRKLHLFMNEKKYFKLGNAGLPVFDIGICKVGMLVCFDWMFSEAWRILMLKGA
                     DIICHPSNLVLPGLCQKAIPIHSLINRFYIVTANRIGTEDDLTFTGLSTISDPKGDVL
                     YQASQDKEELKIVDIDISLARDKKITSMNDIIEDRRVEEYSELLK"
     gene            complement(4182..4613)
                     /locus_tag="AC159U_03850"
     CDS             complement(4182..4613)
                     /locus_tag="AC159U_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIR3336221.1"
                     /translation="MQKEYSADGHTKPEHPIIFFDGVCGLCNSFVDIIFKADNKGIFQ
                     FSPLQGETAKKYITEIPEEAGEWSIVYVDEDSIYEQSDATLKILKRLGGIWCILSMFS
                     FLPRGFRNYIYRLVAVNRYKIFGKRETCRIPTEEEKARFLP"
     gene            4713..>4941
                     /locus_tag="AC159U_03855"
     CDS             4713..>4941
                     /locus_tag="AC159U_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336222.1"
                     /translation="MIKNIILLLVILSIPTSSYGMEYKPVDGTDIIGTKAPEFEGLKW
                     LNTEPLTIDQLKGKVVLIRFWLVGCPYCTNSA"
BASE COUNT         1583 a          843 c          903 g         1612 t
ORIGIN      
        1 ggcacttcca atacaaacat ttgtattagc ttgcttatct tactatttga tagttttgta
       61 ttcttaagta acttcttttg catagtttca gcttagtata ctttgctgtc ctatgcgagc
      121 tccttaaata ataatgaact aaatttattt acagatgaat tcagatcgcc aatatttgga
      181 gacgatctgg caatggcaat tttggagtta gctgatactg aatatagagg acttattaat
      241 atagcaggac caaaggcgat caaccgttat gatttgggat gcaggatagc caaatatttt
      301 ggcctgaaga ctgatttact acgacctgtt gaagctaaag agttaaattt gaacaggccg
      361 cttaattgta ctcttgaaag ttctaaagca acaaatattt taagcactct tattaaaccc
      421 atcagtaaaa catttactta acatttattg gctcaataca ctcagaccct tcattctctg
      481 ctctgtttac atagtttgat atctcgtaaa cctccatttg ttcggatgga taagttctta
      541 acagaggtat aacctcggca gggtcttgaa tgttcgaatc gagccatttt tgttcatcct
      601 ctttgtttag tatgacgggc atacggtcag agattggatt tataagatca tttgaagttg
      661 tagtaactaa agtaaatgtg gttaggtttc cgccatctct tccccaaata tcccatagac
      721 cggcaattcc aaaaatgtct tcattcttat gcctaataaa atatccaacc ttagttgttc
      781 tatctattct tttgcaaaca tagtatccgc tgcagggaac gatacatctt ttatttctga
      841 gtgaattctt gaaattcttg tcttggttga ttgtctctag ctttgcattg attaattgaa
      901 aaccaattag tggatcgtta gaccagccag gaactaatcc ccatctcata atgatacatt
      961 ccgtttcatt attgtttgtt ataatgggaa tgttttgacc ggggtttaca ttgtatctcg
     1021 gctcatgtct aatagatccg gttcttaagc caaacgtttg ttcaattaat ttcgtattgt
     1081 gatacaaagc tattttctga ggcataattt tatttaagaa attttaattc ctttctaaaa
     1141 aagtatttga cttctgccgt gcctgagcat gctctatcat atgcattaca aggtctgcga
     1201 aggtttttaa gagaagaaat aaaaccagag caataacaga cccttttgca aataatcctc
     1261 cgaagatgat tgctatatgc ataggaacaa ttcgcaggta tggaaaaatc atcacagtgc
     1321 cgatgtttgg aactctattc atatctcttt cacggttatg tatgtaggaa tataggtggt
     1381 ttgatagaaa tactgatata catactaaaa tatatattag tgaatctttg tttaaatgat
     1441 caacagaatc aaatataaaa attaaataaa caaggtgaaa aattccataa tgcatagcga
     1501 aaaaattagc ggcagttttt tgtgtctttt tggtgggttt ggttggtttg ttgtttaact
     1561 ggaaaccggt tgtagagaac ttttttaggt ctaagattct tttgcgatta aaaaatccaa
     1621 ttattacact ctgcccccaa taaatccaca taagctctga aaggttccat ttttcatata
     1681 ctgcaataaa gatcgttatc agatttgaaa ttaaaagaaa tacagcagag ctatctatat
     1741 gaaagtgttt gcggagttct tctattaaat tgtcttgcaa tatcttgcta gaattcatta
     1801 tttaatatta ttgatacatc cagtttaaat tcaaataata gaatcaaaat tctaatacaa
     1861 atatatttat atggtattat tttttgaatc tttgacagga ggtgtggggg atgatgaaga
     1921 aactaatatt ttcagtattt ttaatattaa tatttattgg gtgcgatgag gaacaggtgg
     1981 atggcaacag acctcctatg ataaacagaa tagatatatc acatagtctg gaatccggca
     2041 taaacaggta tgtagttaat actgatgcca ctgacccaga taacgacaac ataacttatt
     2101 cttttgatac gagcagtaat atcactattc ttgcttcaac ttcaactgca cttatcaata
     2161 ttccacaatc tacaggaaca cccccagata ttattatcaa agtaattgca gaagatgaca
     2221 agggtggtat agcgactagg gaattcaact tgcgtaccgg aaataaggca cccgtaattc
     2281 ttttttatga agcagataaa tataaactat caaagggtga aaatactaag cttcatgctc
     2341 ttgtaggcga tgatgatgga agtggagata cttatagtac tacttttcgc atatcgtccg
     2401 ctataacttc tggtatttta aatgtcacca caaataacca gtctttgcca cgggcttcat
     2461 ataccggaag tcaatccaat tctgaaaatg ctactattga actcgaggca attgatactg
     2521 ataacaatct aaaggcaagc agcttcctta cacttgaagt aggaacaggc ctagtacatg
     2581 tagattcttg ggatacttca cccatacagc cttatgggct ggattttagc agtaacggcc
     2641 acctttttat tacaagtatt ggttcaactg taatggaatt ggatacaaac ggcaataagg
     2701 taaatggtaa tggctggaat gtaacagatc catcttcaac acatttgcgt gcattgactg
     2761 ttgataaaag aacgcaaaat gaagttctat ttcttggctc tgatagaggt acacatgagt
     2821 ttataatcaa atatagcaaa accggatcgg cacagaccga taataagact acgtttcccg
     2881 gaataccaca ttatttttat attagaccta acggcacaat tcttatttcc aataataaac
     2941 ccagcagcta cggaattaac atcttaaaca ctaatggcac tatacaaagc tggacatctg
     3001 ccggacaact ttctcagcca aggcaaattt ctctagaccc aaatactaat aatgtctatg
     3061 tagccgatag gggaaatggc agagtaagag tgtttgattc cagtggaagt tttataagaa
     3121 atattactgg aaatagcggg cagtttagca atccgcatgg agtgcttgca gacagggttc
     3181 ataattcagt ttttgtagct gatacggaaa atgacaggat tcaggaattt gcatttgatg
     3241 gaacgttcaa ggccgaattt agatcaaatg atgtaaataa acccagatat attatacgcg
     3301 ggcctgataa cagaatttat gtagctagct atatagataa agagattcat gtatttgagc
     3361 atagatagat aatataaata tgaaaacagg ttttttacaa tttgctccaa agctgaatga
     3421 ttctggtttt aatatccaaa aaatagagag tttcgcagag cagtttaaag atgcggattt
     3481 attggttttg cctgagcttt gcaattccgg ttataatttc aaatcaaaga agcaggcatt
     3541 tgatacttca gaagagatag aaaacagtga atttataaac tttctggaat caatatgcat
     3601 agaaaactct ttgttcatcg tatcaggttt taatgaaaga gatggtggag atttatataa
     3661 ctcatccgtt cttatagggc ccgatggatt tatcggaaat tacagaaagc tccatctctt
     3721 tatgaatgaa aagaagtatt ttaaactagg aaatgcgggg cttccggttt ttgatattgg
     3781 aatatgcaaa gttggcatgc tcgtgtgttt tgactggatg ttttccgaag cctggagaat
     3841 attgatgctc aaaggtgctg atataatctg ccatccatcc aatctggttt tgcccggtct
     3901 atgtcagaaa gctataccta ttcattctct tataaacagg ttttacatag taactgctaa
     3961 caggataggc acagaggatg atcttacatt tacaggtcta tcaacaatct ctgatccaaa
     4021 aggtgatgtt ctttatcagg cttctcagga taaagaagaa ctaaagatcg tagacattga
     4081 tatttcactt gcaagggata agaagattac atctatgaat gatattattg aagatagaag
     4141 agtcgaagaa tattctgaat tattaaaatg aaagaatgtc tctaaggtaa aaaccttgct
     4201 ttttcttcct ctgtaggaat acggcaggtt tctctttttc caaatatctt gtacctgttc
     4261 actgcaacca gtctgtatat atagtttcta aaacctcttg gtaaaaagct gaacatactt
     4321 aagatgcacc agataccacc aagacgctta agtattttta aagtggcgtc ggattgttcg
     4381 tagatgctgt cttcatccac atatacaatt gaccattctc ctgcttcttc tggaatctca
     4441 gtaatatatt tttttgctgt ctctccctga agcgggctga attggaatat acctttattg
     4501 tctgctttaa aaattatgtc cacaaagctg ttgcaaagcc cgcatactcc gtcaaaaaat
     4561 attatcggat gttctggctt tgtatgtccg tctgctgagt actctttttg catcaaatta
     4621 aatattaacc gttatattta aaaaccaaac attattattt tcaaccaatt acactatact
     4681 tattaaataa tcatttattg tgaattgtta agatgataaa aaatataata ctgctattag
     4741 taatacttag tattcccact tcgtcttatg ggatggaata taagcctgta gatggcacag
     4801 acattattgg cacaaaggcc cctgagtttg aggggcttaa atggcttaat actgagccat
     4861 taacaataga tcagttaaag ggcaaagttg tcttgatacg cttctggctt gtaggttgtc
     4921 catactgcac aaactctgcg c
//
LOCUS       DBNGSV010000110         2436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1820377, whole genome shotgun sequence.
ACCESSION   DBNGSV010000110 DBNGSV010000000
VERSION     DBNGSV010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1820377"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC159U_03860"
     CDS             <1..213
                     /locus_tag="AC159U_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336223.1"
                     /translation="KHFDQISGISFLPHSDHIYQQAPYEDLTKEQYDDMLTKMPKEVD
                     WSTMVEDKDNTSSSQNLACVAGACEV"
     gene            239..430
                     /locus_tag="AC159U_03865"
     CDS             239..430
                     /locus_tag="AC159U_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336224.1"
                     /translation="MLNITRDECVSCGRTIQEIIDAGTRRRVSNEPEATEDNEGDSYP
                     DKEAGSGSEGGSGTAMDLP"
     gene            529..912
                     /locus_tag="AC159U_03870"
     CDS             529..912
                     /locus_tag="AC159U_03870"
                     /inference="COORDINATES: protein motif:HMM:NF022151.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HIR3336225.1"
                     /translation="MKLAIIGSRTIKDEQIVWNAIYNFIRDHVFEGTAVTLISGGADG
                     VDNFARSYAKKWCLDHVEFIPYFKLDDNASYSARHFFIRNKQIIDNADKVLAIWNGHS
                     TGTKHAIKYAQKTGKPIMVIKKHEH"
     gene            complement(1129..1314)
                     /locus_tag="AC159U_03875"
     CDS             complement(1129..1314)
                     /locus_tag="AC159U_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336226.1"
                     /translation="MEFTVEAYILFVLAQIVTAAAIWGGLRADIKNIHSAMLHNEKAI
                     SDAHSRIDTILMRKLDL"
     gene            complement(1319..1528)
                     /locus_tag="AC159U_03880"
     CDS             complement(1319..1528)
                     /locus_tag="AC159U_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336227.1"
                     /translation="MAISVLGTAGTVMFANHDRSTENATKHIEEVKRSTGVDETQRFF
                     IEHNHNKMENHAERISALEALIKRK"
     gene            complement(1548..2267)
                     /locus_tag="AC159U_03885"
     CDS             complement(1548..2267)
                     /locus_tag="AC159U_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336228.1"
                     /translation="MKGKEGFLGVQKNKAGEDVTERSVEVGIDGIDISIPLMVPSLTA
                     EEIELVVEDKPVTNEIIDKAIVHARERIRQGKSVFKENPSATIAEARAGSGKPNDNNP
                     LNLRYGGGSKKFREFPSLNDGLRSSAHQIYRYYTGATINKKLRTPAEITGLWNNEKEK
                     GSSSASNYLKAVVQHSGLNPNEKIDIDDVADVASLMYGMAKAENHKTKVTERQIRKAL
                     QEKDWKEQGKKLNIAESTKDV"
     gene            complement(2206..2391)
                     /locus_tag="AC159U_03890"
     CDS             complement(2206..2391)
                     /locus_tag="AC159U_03890"
                     /inference="COORDINATES: protein motif:HMM:NF023980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HIR3336229.1"
                     /translation="MTASPTDDRIVFIHGPENPTPEWSYAAWHRRGVIVHGARWYIER
                     KRRILRCSEEQGRRRCY"
BASE COUNT          727 a          474 c          469 g          766 t
ORIGIN      
        1 aagcatttcg atcagattag tggtattagt ttcttgcctc attcagatca tatctatcag
       61 caagctccgt atgaggactt gacaaaagag cagtatgatg atatgcttac taaaatgcct
      121 aaagaagtag attggagtac aatggtagag gataaggata atacatcatc cagccaaaat
      181 ttagcatgcg tggcaggggc ttgtgaagtc taaggagaag gaatgcatcc ttgaatgtat
      241 gctgaatata accagagacg agtgtgtgag ttgtggtaga acaatacagg agattataga
      301 tgcaggtact agaagaaggg tatcaaatga acccgaagct acagaagata atgaaggaga
      361 cagttatcca gataaggaag ctgggtctgg atctgaagga gggagtggta ctgcaatgga
      421 cttaccctga agatcccaac ttgttctttc agttagtaat ggaagaactg ccggatgaaa
      481 tcgtagcaat gaaagagaaa atagacagta aactacatta ggagaagcat gaaactagca
      541 attataggta gtcgtacaat taaagatgaa caaattgtat ggaatgccat atataatttt
      601 atcagagacc atgtatttga aggtactgct gtaactctta tatctggtgg ggctgatgga
      661 gtagataact tcgccagatc ttatgctaaa aagtggtgct tggatcatgt tgaatttatt
      721 ccatatttta aactggatga taacgcttct tattcagcaa gacacttctt tatacgtaat
      781 aaacagatta tagacaatgc tgataaagta ttagctattt ggaatggaca ttctactggt
      841 actaagcatg ccattaagta tgctcaaaaa acaggcaaac caattatggt gataaagaaa
      901 catgaacact aaagatattg tacttcatgt acatttatat gaagtatatg agaaaataga
      961 ggaactaatg gaaagagtag aaacattaga aaaacatgtg ggaacattag agtctgagat
     1021 acttattctt aaatgtcgta gacctataga tgatagttta ccacgaccac cttgagtctt
     1081 ttaaattacc agaaggtatg tggggaagag tttccagagt ctaagacact acaaatcaag
     1141 cttcctcatt aatattgtgt ctattctgct atgtgcatca ctaattgctt tttcattgtg
     1201 gagcatggca gagtgaatat tcttaatatc agcacgtagc cctccccaaa tcgcagcagc
     1261 agtcacaatt tgtgccaata caaataatat atatgcttcc actgtaaatt ccatactatt
     1321 acttcctctt aattaatgct tcaagtgcag agatcctttc agcatgattt tccattttgt
     1381 tatggttatg ttcaataaag aatctctgag tctcatctac tcctgttgaa cgcttaactt
     1441 cttctatatg tttggtagca ttctccgtac ttctatcatg gttagcaaac attactgtac
     1501 cagccgtacc taatacggat atagccatag ttataattac tttattgtca aacatctttg
     1561 gtagactcgg ctatattgag ctttttacct tgttctttcc aatccttttc ctgtaacgct
     1621 tttctaattt gcctttctgt tacctttgtt ttatggtttt cagctttagc cataccatac
     1681 attaatgaag ctacatcagc aacatcatca atatcaatct tctcattagg attaagacca
     1741 ctatgttgaa caactgcttt cagatagttg gaagcacttg agctaccttt ctctttctca
     1801 ttattccata gacctgtaat ttctgccgga gttctaagtt ttttatttat agtagcacct
     1861 gtgtagtatc tgtatatttg atgtgcagag ctacgtagtc catcattcag tgatgggaac
     1921 tctctgaatt tcttagaacc tccaccatat cttagattta gtggattgtt gtcattaggc
     1981 ttaccactac ctgctcttgc ttctgcaatg gttgcgctag ggttttcctt gaatacggat
     2041 ttaccttgac gtatacgttc tcttgcatgg acaatagcct tatcaataat ttcattagta
     2101 acaggtttat cttccactac aagctctatt tcttccgcag ttaaacttgg aaccattaga
     2161 ggtatactta tgtcaattcc atcaataccc acttctacac tcctttcagt aacatcttct
     2221 cctgccttgt tcttctgaac acctaagaat ccttcttttc ctttcaatgt accatctggc
     2281 cccgtgaacg atcacaccgc gccgatgcca cgcggcatag ctccactccg gcgtcggatt
     2341 ctccggcccg tggatgaaaa cgatgcggtc gtccgtgggc gaagccgtca cgacaccgca
     2401 gcaggcgccg tccgttgacg tgaacaatgt ttcaat
//
LOCUS       DBNGSV010000111         2136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_990251, whole genome shotgun sequence.
ACCESSION   DBNGSV010000111 DBNGSV010000000
VERSION     DBNGSV010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_990251"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="AC159U_03895"
     CDS             <1..1019
                     /locus_tag="AC159U_03895"
                     /inference="COORDINATES: protein motif:HMM:NF023428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3336230.1"
                     /translation="DSFRFGKKTVLFIDEIHRFNKAQQAALLKSVEEGTVILIGATTE
                     NPSFEVISPLLSRSQVYVLKQLSESDLETILANAITTDKVITQISLSNEAKKELIRFC
                     GGDARTLLNAFELSCNLAKAEDKTKIDVDIVKKAYQREHLLYDKKGEEHYNTISAYIK
                     SIRGSDPDAAVYYLARMLEAGEDPKFIARRLIVLASEDIGNAEPYALTLATSCFSAVD
                     YIGMPEASLVLSQTSTYLASCPKSNSSTIAISKASKEVKNRPNLSVPLHLRNAPTNLM
                     KDLDYGKDYKYSHDFDEHISDQQYLPDELKNKIFYEPTELGREANIKKYLDKVRIKKN
                     KKNK"
     gene            complement(1022..1402)
                     /gene="gloA"
                     /locus_tag="AC159U_03900"
     CDS             complement(1022..1402)
                     /gene="gloA"
                     /locus_tag="AC159U_03900"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIR3336231.1"
                     /translation="MKFLHTMIRVGDLDRSINFYTEVLGLKFHRKTEYPDGKFTLAFL
                     GYGGNTEPFLELTYNWDTESYDLGNAYGHMAFGVEDIYATCDKIEELGGKVTRPPGPM
                     KHGTTVIAFIEDPDSYKVELIERK"
     gene            1482..1832
                     /locus_tag="AC159U_03905"
     CDS             1482..1832
                     /locus_tag="AC159U_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336232.1"
                     /translation="MAHPAIKVSIIVMAISVIYAYILQIKKDNKADKLRKWVMEKYPE
                     VYDSQPWHHKKMLKSEVVLNVINKKKLIDDEEFKNMYSDVNANNKKFYILLALGIACI
                     AFAVIGSKYFGLEL"
     gene            1894..>2136
                     /locus_tag="AC159U_03910"
     CDS             1894..>2136
                     /locus_tag="AC159U_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336233.1"
                     /translation="MLKLLIILMMLCINAYAFDQDIPNPVKVEIITNQSELIGGEDHK
                     IGVHFKLDPGWHIYWKNSGDSGLPTRVSFTLPDGILL"
BASE COUNT          750 a          366 c          399 g          621 t
ORIGIN      
        1 aggactcatt tagatttgga aagaaaacag ttttattcat agatgagata cataggttca
       61 ataaagctca acaggcagct ctactaaaaa gtgtagaaga agggactgta attctcattg
      121 gcgctacgac tgaaaatccg tcattcgagg taatctcacc actactatca agatcacagg
      181 tttatgtttt aaagcagctt tcagaaagtg accttgagac tattttggca aatgcaataa
      241 caaccgacaa ggtgattact cagatttcac tttctaacga agcaaaaaaa gaattgataa
      301 gattttgcgg aggggatgca agaactcttc taaatgcttt tgagctttca tgcaacctgg
      361 ctaaggcaga agacaagaca aagatagatg tggacatagt caaaaaagcc tatcagagag
      421 agcacctgtt atacgataag aaaggtgaag agcactacaa tacaatttct gcatatatca
      481 agagcattag aggaagcgac cccgatgcag cagtgtatta tcttgcaaga atgctcgaag
      541 ccggggaaga cccaaagttt atagcaagaa ggctaattgt tcttgcttca gaggatatag
      601 gcaatgccga gccctatgcc cttacccttg caacatcttg ctttagtgct gtggactata
      661 taggcatgcc cgaagcaagt cttgtactct ctcagacatc tacatacctt gcaagctgtc
      721 caaaaagcaa ttcatctacg attgcaatat caaaggcatc taaagaggtt aagaacagac
      781 caaacttatc agtgcctctt cacctgagaa atgctccaac taacctcatg aaagatcttg
      841 attatggtaa ggactataaa tactcacatg attttgatga acatatctca gaccaacagt
      901 acctgccgga cgaactaaaa aataaaatct tctacgaacc cacggagctt ggaagagaag
      961 ctaatattaa gaaatatttg gataaggtca ggattaagaa aaataaaaag aataaatagt
     1021 attatttcct ctcaattaac tccactttat aagaatcagg gtcttctata aatgcaatca
     1081 cagtagtgcc gtgtttcata ggaccgggag gccttgttac cttgcctcca agctcttcaa
     1141 ttttgtcaca tgtagcatat atgtcttcta ctccaaatgc catatgtccg taggcattgc
     1201 caagatcata gctttcagta tcccagttat aggttagttc taaaaagggt tcggtgtttc
     1261 ctccgtatcc taaaaaagcc agtgtgaatt tgccatcggg gtattcagtt tttctgtgaa
     1321 atttaagccc aaggacttct gtgtaaaaat taatggatct atccaggtca ccaactctta
     1381 tcatggtatg taaaaatttc atataatttc tcctttaagg taataaataa aatatcattt
     1441 ataattatat agataataaa ttataaacaa agagaattat tatggcacat ccggcaatta
     1501 aggtctcaat cattgttatg gcaataagcg ttatctacgc atatatcctt cagattaaaa
     1561 aagacaacaa agctgataag cttagaaaat gggttatgga gaaatatcct gaagtttatg
     1621 attcacaacc ctggcatcac aaaaaaatgt taaaaagcga agtagtactt aatgtcataa
     1681 acaaaaaaaa gttgatagat gatgaggaat ttaaaaatat gtatagtgat gtaaatgcta
     1741 ataataaaaa gttttatata ttacttgctc tgggtattgc ctgtattgca tttgcagtaa
     1801 ttggttcaaa atattttggg ctggaacttt aggaaattta ttgagtcttt tcattaatta
     1861 tctcatcata gagtaaaaat gataaatagt tatatgttaa aattactaat aatattgatg
     1921 atgctttgca taaatgcata tgcatttgat caggatatac caaatccggt taaggtagaa
     1981 ataattacga atcaaagtga gttaataggc ggagaagatc ataagattgg ggttcatttc
     2041 aagcttgatc ccggctggca tatatactgg aaaaattccg gagattcagg gcttccaaca
     2101 agagtaagtt ttactctgcc tgatggaatt ctatta
//
LOCUS       DBNGSV010000112         7088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1682561, whole genome shotgun sequence.
ACCESSION   DBNGSV010000112 DBNGSV010000000
VERSION     DBNGSV010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1682561"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1426
                     /gene="thrC"
                     /locus_tag="AC159U_03915"
     CDS             92..1426
                     /gene="thrC"
                     /locus_tag="AC159U_03915"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3336234.1"
                     /translation="MNYKAWFQCIDEECGQTYELNEIVYRCKSCNNLLEVVHDMEVLK
                     QTSAKEWKELFDSRYRRQSWPFGSSVWGKKEWVCPYVESDNVVSMYEGATNLFWAERF
                     GKQVGIEDLWLKMCGNSHTGSFKDLGMTVLVSVVKQMISEGKNIPAVACASTGDTSAA
                     LAAYCASAAIPSVVFLPKDKVSIAQLVQPLSNGALVLSIDTDFDGCMEFVQKICTEQN
                     IYLANSMNSLRIEGQKTISIELCQQFQWDIPDWVIIPGGNLGNISALGKGFLMMYELG
                     LIDRLPRLVCAQAESANPLYLSYKKGFKEFHPVKAKTTYASAIQIGNPVSINKAIKTL
                     KTFDGIVEQASETELADAAAMVDKTGTFNCPHTGVALAAFLKLKERNLFKPSDKIVII
                     STAHGLKFVDFKIGYHKDQLEGLNPSFSNRPIELPYDFDAVKDAIFSNIDIS"
     gene            complement(1543..2412)
                     /locus_tag="AC159U_03920"
     CDS             complement(1543..2412)
                     /locus_tag="AC159U_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336235.1"
                     /translation="MIRQQTGEKIYKYIIFSVLLIVLTVFSVTKDARSAGVDLPQSFF
                     LEHPYSITGVKISTEIIDLDIEDALYMNEIRADVNIHDNKASLYTRIPIAGITDVDPT
                     NEDDIALGNIVIGGKSTIFQGNTAVLTGGIEGFLPTVDNNLAAMGARTYFREYASFVD
                     DAFTIRPYAVFGVGKDIFAFQANLGADIITGAEEIEGDDTEVIWRYGGTASVTPHMPV
                     PFATSFLVEVLLASASSFEDSRTEAFITPGFRFGGQIFSVGAGVEIPVGEESDEFADD
                     VGILLDLLIRFGS"
     gene            2552..3193
                     /gene="thiE"
                     /locus_tag="AC159U_03925"
     CDS             2552..3193
                     /gene="thiE"
                     /locus_tag="AC159U_03925"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3336236.1"
                     /translation="MKHKLKGLYVITDKKLIERSNFVQTVEKALAGGANVLQLREKET
                     DHDEIVYLGKELLKISSKYSVPLIINDSPEIAKEIGADGVHLGRQDANIENARKIMGD
                     DKIIGVSCYGELERGLDARARGASYLAFGTPYNTPTKPGREPTPFSNLIEAKNQIPDI
                     PIFAIGGIYKENAQDVLDTGVDGIAVITSVFGSEDAEAASRELSSIINNQKSL"
     gene            complement(3204..4247)
                     /locus_tag="AC159U_03930"
     CDS             complement(3204..4247)
                     /locus_tag="AC159U_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3336237.1"
                     /translation="MNKTKAVFIFSFVVIVAFILILSIYLTKAKNQKLTYLYGLGEKV
                     SRSANEEPPTAAIEIEFNKDDSKIITLLNNGDVEQWDLTKKTKESVAKTNRVFSYCAD
                     KDMLVTSENDTIYLTDIVSRNKRSLTRGKYQLASVDKKCEYLALIGDGSEEVYVWNLK
                     SNSLKAKYRTKLPARNGLALSPDGSKVAVAEGVYHEDKRMHETLIEVWDISSGNKEPI
                     LKYDKRESGLIVGVWNIFFSSDSNKIVFDTQHQAESGIRLIDLQGNTIFEESGFKSYW
                     MRASAFNSDKGHLATGDEERNVAIWNISSKAIGFYNKMPDVVESVSFSHNGSLIAAGI
                     ADSTIQVFSTEKQ"
     gene            complement(4291..5454)
                     /locus_tag="AC159U_03935"
     CDS             complement(4291..5454)
                     /locus_tag="AC159U_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3336238.1"
                     /translation="MGTKTDFLIIGAGPFGLSMTAYAKHLNIDYKIVGRTMEFWKKNM
                     PAGMRLRSGPDWHLDPLGEYTLVNFLKTKNISHENISPIPINLYLEYVDWFRKEKQIE
                     PIQEYITKLDYYADKKHRFRAYLSNNEVIEAQFVLVAIGFGYFKNIPQEMSSSLPNSY
                     YSHTADLIDFSKLKSKNCIIVGGRQSAFESALLMKEAGVREIDIVHRHKTPEFTHSDW
                     GFVTPILDKMIDDPDYYIKLSDLEKKEINDRFWQEGRLKLEPWLKELDQYEDIGINEQ
                     TNVCSSHITKDGNIEVILSNGTNLNADHLLFATGYKVDLQRVPFLKNGNILDKLATNN
                     GFPKLKAKFQSSLPGLYFTSLPATADFGAFFGFTVSCNTSSMIIGNEIRNISS"
     gene            complement(5438..6130)
                     /locus_tag="AC159U_03940"
     CDS             complement(5438..6130)
                     /locus_tag="AC159U_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3336239.1"
                     /translation="MLNNLIQIAGVINLEEALMLIDECIEYIGFPLRLKDGREDLSEQ
                     SAREIIKYIKPISKPVLITYQQNAKEISEFYEYLEVDIIQLHGHIKLEEVRNLKKLYP
                     DLLIIKSLIVRNDNLEEITDEINMFSDFTDYFITDTFDPKTGRTGATGKTHDWEISRQ
                     IVNISPKPVILAGGLNPENVRDAINVVKPAGVDTHTGVENLSGKKDAELVKKFVSESQ
                     KGFNDLLNGDKD"
     gene            complement(6392..6745)
                     /locus_tag="AC159U_03945"
     CDS             complement(6392..6745)
                     /locus_tag="AC159U_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336240.1"
                     /translation="MFFVFTEDGCLEIVDTIKEIRTNYEGIDVESGVYEFFDQKGSRL
                     KPRFSKPNKVKKYFGLFSTVESGQFDLIADVDPGSDSIFTLLKETSHLKSNKHFKSLA
                     NVKMYLEAGNESKKD"
     gene            complement(6764..>7088)
                     /locus_tag="AC159U_03950"
     CDS             complement(6764..>7088)
                     /locus_tag="AC159U_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336241.1"
                     /translation="EFGGIYSYLVYKVEYDKQANQEVFTIRQPAGGGALELAGHYMNV
                     EIVEPEKLMERVFAEQSGKIASQQPPATSKPAADKTVVQTMRIEEVLKDGKNSGKETE
                     TKKEE"
BASE COUNT         2175 a         1378 c         1240 g         2295 t
ORIGIN      
        1 ttaacataaa tatttgattt gactgtactt tataatatct aaaaagaaaa attgacagct
       61 aaatctacta tatgtatttt gtactaatat tatgaactac aaagcatggt ttcagtgtat
      121 tgatgaagag tgtggccaga catacgaact taatgaaata gtataccgct gcaaatcctg
      181 caacaacctc ttagaagtag ttcatgatat ggaggttcta aaacaaacat ctgccaagga
      241 gtggaaggaa cttttcgaca gcagatatag aagacagtcc tggccgtttg gaagttcagt
      301 atggggaaag aaggaatggg tatgcccgta tgttgaatca gacaatgttg tctctatgta
      361 tgagggtgcg acaaacctgt tctgggcaga gagatttgga aagcaggttg gtattgaaga
      421 tctctggcta aaaatgtgcg gtaacagcca tacaggttct tttaaagacc ttggaatgac
      481 agtgcttgta tctgtagtta agcagatgat tagcgaaggc aaaaatatcc cggcagtagc
      541 ctgcgcttct acaggtgata cttctgctgc tcttgcagct tactgcgctt ctgccgctat
      601 tccttcagtg gtttttcttc caaaggataa ggtttccatc gctcagcttg tacagccgct
      661 ttcaaacgga gctcttgtac tttcaataga caccgacttt gacggttgta tggaatttgt
      721 tcaaaaaata tgcaccgagc aaaatatata tcttgcaaac tcaatgaatt cactaagaat
      781 cgaggggcaa aaaactatta gtattgagct ttgccagcag tttcagtggg atataccaga
      841 ctgggtcata atcccagggg gaaatcttgg taatatctct gcgcttggaa agggattttt
      901 gatgatgtac gagcttggac ttattgatag actgccaagg cttgtatgcg cacaggcaga
      961 gagtgcaaac ccactttatt tgagctataa aaaggggttt aaagaattcc atcctgtaaa
     1021 ggcaaaaaca acatacgcaa gcgctataca gattggaaac ccggtgagta taaacaaagc
     1081 cataaaaaca cttaaaacat tcgatggtat agttgagcag gccagcgaaa cagaacttgc
     1141 cgatgctgct gccatggtgg ataaaacagg cacattcaac tgccctcaca caggggttgc
     1201 tcttgcggca tttctaaaat taaaagagag aaaccttttt aaaccttctg ataaaatagt
     1261 aattatttca actgcacacg gacttaaatt tgtagatttt aagatcggct accataaaga
     1321 tcagcttgaa ggactaaacc ccagttttag caacaggccc atcgagcttc cctatgactt
     1381 tgatgccgta aaagatgcaa tattctctaa tatcgatatt tcctaaaaat tattaaatac
     1441 aaaataatac gttataattt taaatgagta ggccatgacc ggaataatat tcgcagtcat
     1501 ggcctgttcg atgggggaaa aacaacttaa gttttaaatt tattaacttc caaatcttat
     1561 tagtaagtcc agtagaatac ctacgtcatc agcaaattca tcggattctt cgcctaccgg
     1621 aatttcaaca cctgctccaa cactaaatat ctgtccgcca aacctgaacc caggtgtaat
     1681 aaaagcttct gttctgctgt cttcaaacga actagccgaa gctagtagta cctcaaccaa
     1741 aaatgaagtt gcaaaaggta ccggcatatg aggagttaca cttgctgtac ctccatatct
     1801 ccatattact tcggtatcat cgccttcaat ttcttcagcg cctgtaataa tatccgcacc
     1861 caggtttgcc tgaaatgcaa atatgtcttt accgactcca aaaaccgcat aaggtcttat
     1921 ggtaaaagca tcatcaacaa atgaggcgta ttccctaaaa tatgtacggg ctcccatggc
     1981 agctaagtta ttatccactg ttggtagaaa tccctcaata ccacctgtaa gaactgctgt
     2041 attgccctga aatattgtgg atttaccacc gatcactatg tttccaagag ctatgtcatc
     2101 ttcatttgtt ggatcaacat ccgtaatccc tgcaattggt attcttgtat ataatgaagc
     2161 tttgttatcg tgaatgttaa catctgccct aatctcattc atgtaaagag catcttcaat
     2221 gtcaagatct ataatttcag tactaatttt aacacctgtt atagaataag ggtgctctaa
     2281 aaagaaagac tgcggtagat caaccccggc cgaccgggca tcttttgtta ctgaaaaaac
     2341 agttaaaact atgagtaaaa cagagaatat gatatattta taaatctttt cgccagtttg
     2401 ttgtctaatc atcctccaca acctccatct caatcttaaa aattaatttg taatgatatt
     2461 aatacatttt gattaataat tcaagtagat tattaaatat ttttttaaac attgtcataa
     2521 ctcccctaat ttgttatttt attttttata tatgaaacat aaattaaaag gtttatacgt
     2581 aataactgac aaaaagctaa ttgaacgatc taattttgtg cagacagtag aaaaagcgct
     2641 tgcaggagga gctaatgtgc tgcagcttag agaaaaagag actgatcatg acgaaattgt
     2701 ttatttgggg aaggaactac tgaaaatatc atcaaaatat tctgtccctt taataattaa
     2761 tgatagcccg gagattgcaa aagaaatcgg tgcagatggt gttcatctgg gtaggcaaga
     2821 tgccaatatt gaaaatgcaa gaaagatcat gggagatgat aaaataattg gagtatcctg
     2881 ttatggtgag ttagaaagag gtctggatgc ccgcgctcgg ggagcaagct atctggcctt
     2941 tggtacccct tataacactc caacaaagcc cgggagagaa cctacgccat ttagtaattt
     3001 aattgaggcc aaaaaccaaa ttcccgatat tcctattttt gcaattggcg gaatttataa
     3061 agaaaatgct caggatgtac tggataccgg agttgatgga attgcagtta ttacaagtgt
     3121 atttggttct gaagatgcgg aagccgctag cagggaactg tcctcaatta ttaataatca
     3181 aaaaagtctt taaataataa atattattgc ttttcagtac taaaaacctg tattgtagaa
     3241 tcagcaattc ctgcggcaat taaagaacca ttatgggaaa atgaaactga ttctactaca
     3301 tctggcattt tattataaaa ccctatggcc tttgaactga tattccatat agctacattc
     3361 ctttcttcgt caccagtagc aagatggcct ttatcgctat taaaagcaga ggctctcatc
     3421 cagtatgatt tgaaacctga ttcttcgaaa atagtatttc cctgcaggtc tataagccta
     3481 ataccggatt cagcttgatg ctgcgtatcg aaaacaattt tattactatc tgaagaaaaa
     3541 aagatattcc aaaccccgac aatgagtccg ctttctcttt tatcatactt aagaataggc
     3601 tctttattac ctgaagaaat atcccagact tcgatgagtg tttcatgcat acgtttgtct
     3661 tcatgataca caccctcagc cacagcaact ttgcttccgt cgggagataa ggctagtccg
     3721 ttccttgctg gaagcttagt cctatatttt gccttgagcg aatttgattt cagattccaa
     3781 acataaacct cctcactacc atcaccaatt aaagctaaat attcacattt tttatctacc
     3841 gatgccaatt gatatttacc tctggttaag gagcgtttat tacgcgaaac aatatctgta
     3901 agatagattg tatcattctc acttgttact aacatatctt tatcagcaca ataactaaat
     3961 acccgatttg tttttgcaac cgattcttta gtctttttag ttaggtccca ctgctcaaca
     4021 tcaccattat ttagaagagt gataatttta gaatcatctt tgttaaattc aatttcgata
     4081 gccgcggtcg gcggttcttc gtttgcagat ctggaaactt tttctccgag accatacaga
     4141 taagtaagct tttggttctt tgcttttgta agatatatag aaagaatcag gataaaagca
     4201 actattacca caaacgagaa tataaaaacg gctttagttt tattcatacg gttgtgattg
     4261 taataaatta ataaaaattg gcaattaagt ttacgagctt atatttctaa tctcatttcc
     4321 aataatcata gaagacgtat tgcagctcac cgtaaatcca aagaaagctc caaaatctgc
     4381 tgttgcagga agacttgtaa aataaagtcc cggtaggctg ctttggaatt ttgcttttaa
     4441 ctttggaaag ccgttatttg tagccaattt atctaaaata tttccgtttt ttagaaatgg
     4501 aactctttgc agatcgacct tgtatccggt tgcaaacagc aggtggtcag cattgaggtt
     4561 agttccattg ctgagtatga cctctatgtt tccatctttt gtaatatgcg acgaacaaac
     4621 atttgtttgt tcattaattc caatatcctc atattgatcc agttctttta accagggctc
     4681 gagtttcaat cttccttcct gccaaaatct gtcatttatc tccttttttt ccaggtcact
     4741 taatttaata tagtagtcag gatcgtcgat cattttatcc agaattgggg taacaaagcc
     4801 ccagtctgaa tgtgtaaact ccggagtttt gtgcctatgg actatatcaa tttcccttac
     4861 acctgcttct ttcattaata atgcagattc aaatgcactc tgtctgccgc cgactattat
     4921 acaattttta gatttaagtt ttgaaaaatc tattaggtca gccgtgtgtg aatagtaact
     4981 attgggcaaa ctggaactca tttcctgcgg aatattttta aaataaccaa aaccaattgc
     5041 tacaagaaca aactgagctt ctattacttc attattagag aggtaggccc taaatctatg
     5101 ttttttatct gcataataat ccagctttgt tatatattct tgaataggtt caatctgttt
     5161 ttcttttctg aaccaatcga catattccaa atatagattt attgggatcg gacttatgtt
     5221 ttcatggctg atgttcttag tttttagaaa attaacaagt gtatactcac cgagcgggtc
     5281 aagatgccag tccggtcctg acctgagacg cattccagca ggcatatttt ttttccaaaa
     5341 ttccatggtc ctgcctacaa ttttgtagtc aatattcagg tgctttgcat aagccgtcat
     5401 agacaggcca aaaggtcctg cgcctatgat cagaaagtca gtctttgtcc ccatttagta
     5461 aatcattaaa acctttttgt gactccgaca caaatttttt aaccagttca gcatctttct
     5521 taccgctcaa attttctact cctgtgtgtg tatccactcc ggctggctta actacattaa
     5581 tagcatccct cacattttca gggtttagtc caccagcaag tatcacaggc tttggtgaga
     5641 tatttacaat ttgcctgctt atttcccaat cgtgtgtttt acccgtagct ccggttcttc
     5701 cagtttttgg atcgaatgtg tcagtaataa aatagtctgt aaaatctgaa aacatattta
     5761 tttcatctgt tatttcttct aaattatcat ttcttacaat caggcttttt atgattagca
     5821 ggtctggata aagtttttta agatttctaa cttcttctaa ttttatatgc ccatgaagct
     5881 ggataatatc tacctctaaa tactcataaa attctgaaat ttctttagca ttttgctggt
     5941 aggtgattag aacaggtttt gatatgggtt taatatactt aataatttct ctggcacttt
     6001 gttcagataa atcctctctg ccatctttaa gtcttaatgg aaatccgatg tattcaatac
     6061 actcatctat aagcattaga gcttcttcaa gattgatgac accggcaatt tgaatgagat
     6121 tatttaacat ttatgactct ttaatgagtt tctaaacacc attttgaaag taaatactaa
     6181 acaaatcaca aaaatcagaa ttgctccaat taactctatt tgtctaataa cagaaggtga
     6241 tttagtccca attagatttt caataaccgt ataacatcca tattctctgc ccgtgacatt
     6301 atcagccgga tatagcgaaa atgtgtttgc atcaataaag agtagtaatg aacataggaa
     6361 taataagaag atgtagataa atttattaat actaatcctt tttagattca ttacctgcct
     6421 caagatacat tttaacattt gcaagggatt taaaatgttt gtttgatttt aaatggctgg
     6481 tttcttttag gagagtaaaa atactgtccg agccagggtc aacatcggca attaggtcaa
     6541 actgccctga ttccactgtc gaaaataatc cgaaatattt tttaacttta ttgggtttag
     6601 agaacctggg ctttaatcta ctgccctttt gatcaaaaaa ctcatacacc ccgctttcta
     6661 cgtctatccc ctcatagttt gttctgatct ctttaattgt atcaacaatt tctaaacaac
     6721 cgtcttcggt aaatacaaag aacatacgag agaattttta aatctactct tcttttttag
     6781 tctctgtttc tttaccgcta ttcttgccgt cttttaagac ttcttctatc ctcatcgtct
     6841 gaacaacagt tttgtctgcg gcaggtttac ttgttgctgg aggttgttgt gaagcaatct
     6901 tgccggattg ctcggcaaat actctttcca taagtttttc aggctctacg atttctacgt
     6961 tcatataatg ccccgcaagc tctaaagctc ctccacctgc tggctgtctt atggtaaaaa
     7021 cttcctgatt tgcctgcttg tcatactcga ccttataaac aaggtaactg tatatgccgc
     7081 caaactcc
//
LOCUS       DBNGSV010000113        16379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_435721, whole genome shotgun sequence.
ACCESSION   DBNGSV010000113 DBNGSV010000000
VERSION     DBNGSV010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16379
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_435721"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..891
                     /gene="nadC"
                     /locus_tag="AC159U_03955"
     CDS             43..891
                     /gene="nadC"
                     /locus_tag="AC159U_03955"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3336242.1"
                     /translation="MIDQILLDLKAVEKIVNLSLKEDIGRGDITTNSIVNDKLKCVAV
                     IKSKDRGVVAGNPVAELVFKKLDPKTKYIPIKKDGDAIKPGDIIAEIRGSVRAVLTGE
                     RLALNLLQRLSGIATLSSRFVEKTKGTGVKILDTRKTTPGLRILQKYAVTVGGCYNHR
                     FGLFDAVLIKNNHITIAGDIEKAVEKARAKNKNKTIEVETSNFREVREALKSGVDIIM
                     LDNMSPDKIKKALEIIDGKAKTEASGGININNIKQYAKTGVDYISVGALTHSAISLDI
                     GLYVLE"
     gene            921..1880
                     /gene="nadA"
                     /locus_tag="AC159U_03960"
     CDS             921..1880
                     /gene="nadA"
                     /locus_tag="AC159U_03960"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3336243.1"
                     /translation="MENKADIIAEIDRLRKEKNAVILAHNYQIPDVQDIADFVGDSLG
                     LSQQAGRTDADIIVFCGVHFMAETASIISPDKKVLIPDLGAGCSLSDTITADQLRDWK
                     KQHPGAVVVSYVNTTAEVKSESDYCCTSSNAVEVVNSIPEDKEILFLPDMFLGAFVAR
                     ETGREINIWPGECHVHADIKGEDIKRTTDEHPDAELVIHPECGCSTSTLYECSNDTFN
                     GKPVKVLSTGGMLKHAKSSKSKEFIIATETGILHTLKKHNPEKQFYAANENAVCKYMK
                     MINLHNVLESLQNEQFEVKVPLPIANKAKNAIDRMLDISAQYQ"
     gene            complement(1874..2962)
                     /gene="ggt"
                     /locus_tag="AC159U_03965"
     CDS             complement(1874..2962)
                     /gene="ggt"
                     /locus_tag="AC159U_03965"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3336244.1"
                     /translation="MGEKIVQKDLAWSLKQINKHGPDAFYNGKIAEKIVSDMNENDGL
                     ITADDLSSYKPVIRKPIYGTYRGYEIYSMPPPSSGGIHLIQILNMLEHFDISNIGHNN
                     SRSVHIIAEAMKLAYADRSKYLGDSDFVQVPISELTSKKYAKYLSKKINLNKSTPSKN
                     IGPGNPYIAGGNDTTHFTVIDKSGNVVSNTYTINFSYGSKIMAKGTGILLNNEMDDFS
                     AKPGVPNAYGLVGGEFNAIEPEKRMLSSMSPTIVLKDKKPFLATGSPGGSRIITTVLH
                     IISNVIDHKMNIATATASPRFHHQWLPDILFMESGFSNDTNKILEKKGHEIKVGRAMG
                     STQSIMKIGDYYYGYSDPRRAGGLTKGY"
     gene            complement(2955..3614)
                     /locus_tag="AC159U_03970"
     CDS             complement(2955..3614)
                     /locus_tag="AC159U_03970"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3336245.1"
                     /translation="MNIKLQIFLFLIGITLFNNQLISDPIITSNAIFQPVIAQNGMVA
                     SDNKEATEAGLKILKDGGNAIDAAVTIGFTMAATYPRAGNLGGGGFMLIYLADSREVI
                     AIDYREKAPKAVTRDMFLDKDGNVDKEKSRHSYLAAGVPGTVAGLSLALEKYGTIPLK
                     KALKPAIKYAKNGLDVNYELSSSLKSVKERMKKSPDAYNTFFKEDGDSYDIGEKIVQN
                     W"
     gene            3682..4080
                     /gene="msrB"
                     /locus_tag="AC159U_03975"
     CDS             3682..4080
                     /gene="msrB"
                     /locus_tag="AC159U_03975"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3336246.1"
                     /translation="MSEKINKTEQEWQKELTPEQYRVTREKGTERPFTGKYNENKEKG
                     KYVCVCCGTELFSSDTKFDSGSGWPSFYKPEKEENVKAETDRSHGMIRTEVMCDKCDA
                     HLGHVFEDGPNPTGLRYCINSCSLDFKKED"
     gene            complement(4084..5268)
                     /locus_tag="AC159U_03980"
     CDS             complement(4084..5268)
                     /locus_tag="AC159U_03980"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HIR3336247.1"
                     /translation="MIKAGVGCSNNPKSLEAAENACRDCLTQAGIEKADFILIFTTFH
                     HRNNYQKILEKVSEITGTHNITGCSATGVLSHFGEIEGEPGIVVLGVCSDSLKAKCFI
                     DSISTDMGQASGNNIINRLIEINTKTKLAMVLADPFSFHHNLFFNSLEHASDIVHIIG
                     ATCSEDPSSPATFQYRDNMVNSSSLSGAIISGNFNYNIGLTQGCKPASKPLRVTECNR
                     NVIMEIDGRPAFEVLKAYVPAHILENPNDLLRIVSVGLCSAVKSDTDSTRNYMVRNLM
                     GVDDNSGIIGIAEDINVGDEIVITLRNPEMAREDLKDMLENLKSQIADMSKVRFGIYF
                     NCCGRGSYFYGNKDIDTAYISSYFPDIPMIGFFGNSEIAPMMGSNHLFTYTGVLTLFS
                     EP"
     gene            5437..6378
                     /locus_tag="AC159U_03985"
     CDS             5437..6378
                     /locus_tag="AC159U_03985"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3336248.1"
                     /translation="MESAKIFSGNSNIPLGNAICEYLGVRPGEIDIHRFSDGEIFINI
                     KESIRGDHVFIIQSTCPPVNEHIMELLIITDALKRASSKEITAVIPYFGYARQDRKVQ
                     PRAPISAKLVAEILLRAGVDRLVTVDLHAGQIQGFFDVPVDHIYAAPIFIDYIKKRFD
                     GEDIVIVSPDAGGMERARAYAKRLHATLAMTDKRRTAPNVAEIMYLIGDVKDKTAIIV
                     DDMVDTAGTAVQAAKSIMAEGAKSVSLCCTHGVLSGKAVQRINDSHFEEVIITDTIPP
                     REETLKCEKIKFLSIADLLGEAIKRITTGESISTLFN"
     gene            6396..7067
                     /locus_tag="AC159U_03990"
     CDS             6396..7067
                     /locus_tag="AC159U_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3336249.1"
                     /translation="MAQSVLNVKRRVRMGKSGSKAIRKEGNIPAVLYGKGSEPESIVV
                     NPAELKEALSTDAGENTLLEMHISDWEGGNETTKLSLLRDVQMDYLKNKSIHLDFLEL
                     DMKQTFSVKIPVKIKGIPIGVREEKGMLEEIVREVEIECLPSDIPNEFVIDVTDLHIT
                     ESIHVKDLEVSDKVTILDDPGSTIVTVLAPRVEKTLTAEEEGEGEEATETEREEKNEE
                     SAGSE"
     gene            7090..7695
                     /gene="pth"
                     /locus_tag="AC159U_03995"
     CDS             7090..7695
                     /gene="pth"
                     /locus_tag="AC159U_03995"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009658166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3336250.1"
                     /translation="MFLIAGLGNPGREYENTRHNVGFLGVDRIAQDIGVSVTKKGFQS
                     FYELGHFGNQKVLLIKPQTYMNNSGNAIREAKEYYKIDTENLIIIHDEMDIPLGRIKI
                     KNGGGSAGHNGIKSIIANIGSQNFPRVRIGVGKPYDRDKVIKHVLSNFNKEESKQLFE
                     VLDSVRDSVYEIITSGIEKAMNRFNIRAEKNIENEIEKEEV"
     gene            7744..9786
                     /locus_tag="AC159U_04000"
     CDS             7744..9786
                     /locus_tag="AC159U_04000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3336251.1"
                     /translation="MSELIASLGVLVSLGVLGYAYIVYGGLKALPEGNDKMKEIATAI
                     HEGAMVFLKSEYKIIGIFAVIIFFLVWFGLGFATALAYACGAGLSCLAGFFGMMSATK
                     ANVRTAQAAASDGQGAALSAAFKGGNIMGLSVAGLGLLGLSVGYALQIGIPENPTGTA
                     LTGFVTNFSGVIGGFAMGASSVALFARIGGGIFTKAADVGADLVGKVEAGIPEDDPRN
                     PATIADNVGDNVGDVAGMGADLFESYVSSVIATIVIAAGMTDHNRIALMAIPIVLITI
                     GSVASILGARYIERKKDDEPQSVLRYGTFFAGGIYLGVSLLVLGLLGLVDGIGVFAVK
                     VWGAAVFGLLSGVAIGLVTEYYTSGAPVKKIAEASQTSVATNIIEGLAVGMKSTAWPV
                     LIIVLAISCSFWFAGLYGIGIAAVSMLATIGITMSVDAYGPVADNAGGITEMSELGDD
                     VRVITDKLDSLGNTTAAIGKGFAIGSAALAALALFAAYTKAVGLDSINLTNSKVVAGM
                     LIGGTIPYFIGALTMGSVGRTAQQMVEEVRRQFREIKGLMEGEAKADYAKCVEISTKS
                     SIKEMIWPGVTAVSAPIIIGWILGPEALGGFLAGALVSGVMLALMMANAGGAWDNAKK
                     YIEEGNLGGKGSDAHKAAVVGDTIGDPFKDTTGPAMNILIKLMSIVSLVVAPALFM"
     gene            9873..10388
                     /gene="rpsF"
                     /locus_tag="AC159U_04005"
     CDS             9873..10388
                     /gene="rpsF"
                     /locus_tag="AC159U_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3336252.1"
                     /translation="MQSYETVFITPVDIAEENLNKILDKIKEAVSKNDGELSKLEKWQ
                     QRELAYPINDLTKGIYYIAEYKANSDVVHDIEHNFKFLREDILRFLTIKTKPAKAKPV
                     KPEASKPQATSAPASAAPKPEASAPVTETPEATATPAPISAEPAAASTDTDNSAFASP
                     NSNSNDKGGQS"
     gene            10388..10627
                     /gene="rpsR"
                     /locus_tag="AC159U_04010"
     CDS             10388..10627
                     /gene="rpsR"
                     /locus_tag="AC159U_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3336253.1"
                     /translation="MESPRDRRYMNRKKYCRFCKNKEEIEYKNVDLLARYITERRKIV
                     PRRVTGVCAKHQRILATAIKRARVLALIPFTVLHK"
     gene            10657..11259
                     /gene="rplI"
                     /locus_tag="AC159U_04015"
     CDS             10657..11259
                     /gene="rplI"
                     /locus_tag="AC159U_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3336254.1"
                     /translation="MKVILTTDVDSVGYMGEIKDVKRGLARNYLLPKNLAVEATPGNL
                     KIWNQKQRIIEKHQIKRKEDAESLASNLGSVTCTIKVKVGEEGKLFGSVTTQNISDDL
                     AQKGYKISKKDIVLDAPIKETGTYDVPVKIHPEVSASVKVVVENEEGEQSSETGDQSE
                     EQATEQAAESSEQENSLESSEEASADENSSEQSETKEQES"
     gene            complement(11281..12081)
                     /locus_tag="AC159U_04020"
     CDS             complement(11281..12081)
                     /locus_tag="AC159U_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HIR3336255.1"
                     /translation="MDSLLRNFILFIFALFLSLLINTPTIAGLLLEQESYLEKNPDKK
                     GKGQIYISDNKIKFVADNDDPIIIFNLNKNKLYIIDQQSKQYVESSPDKYVELVQANM
                     TEQKKALKKDLANMDKAKRVQQLKILEKKRININGDEKLKNWKINKTDQTKEIAGLKT
                     EKFELLEDGKVIQDLWVSNKLGEIDFNKLAEFYSEIQKISQGLYLGPENQNRFSKTLS
                     EIYKIGYPMETVDYNLPGNRVETTLSIKEVELADKDFRPPKDYKKNKI"
     gene            12237..13046
                     /locus_tag="AC159U_04025"
     CDS             12237..13046
                     /locus_tag="AC159U_04025"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006056440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HIR3336256.1"
                     /translation="MAEINTKFAPVGEKEVTKAIVEGFAKEFTEHIESDVIVVGAGPS
                     GLVAAKDIAKKKYKVLLVERMNYLGGGFWIGGYLMNKVTVRDPGQTVLDEIEVPYEEV
                     SEGLYVADGPYACSKLIATTCEAGAKIRNMTMFDDLVYRENGRVAGIVMNWTPIANMP
                     KEITCLDPIAIESKLVIDATGHDAYLASRLSQQGLYKKLPGHGSMWVEKSEEALVEYT
                     GEVHPGLIACGMSVNTIYGLPRMGPTFGGMLLSGKKAAEVAVNILQEQAGG"
     gene            complement(13069..13815)
                     /locus_tag="AC159U_04030"
     CDS             complement(13069..13815)
                     /locus_tag="AC159U_04030"
                     /inference="COORDINATES: protein motif:HMM:NF042804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6775 family putative metallopeptidase"
                     /protein_id="HIR3336257.1"
                     /translation="MNKRPVYLYSDTPSKATNISNIIEHLSKFGIESEYKGDFFEYLR
                     LSTAKQNELNHYLSLTLIDDIEKEKLELCHASQNQNTNNKILYDGYLYISVINKYFLL
                     DSNISDSVIHIIYTDKLLCTFENRRYHARVVLMGHPSFISTSGLVEAPARPREYYFLK
                     AQFLQHGRDLKELDEYFKGQYLEYNDPKTTEILQSYTLQPIMYELTGKPFCENKTCCL
                     FNSHWQKDVLEIQYNGQLCKHHRQLIGNLT"
     gene            complement(13839..14306)
                     /locus_tag="AC159U_04035"
     CDS             complement(13839..14306)
                     /locus_tag="AC159U_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3336258.1"
                     /translation="MAMTTNISDRLVELNIQIPKPVIPLGSYTPALISGNHIYVSGQL
                     PLQNGSLIYKGRVGDEITIEQGYEAARLAAINALSAISTIIDNLDRIKKIVKLNGYVM
                     SSPDFFDQPKVINGASELLFDVFGDIGVHSRAAVGVSNLPMQSCVEIDLIAEI"
     gene            complement(14290..15726)
                     /locus_tag="AC159U_04040"
     CDS             complement(14290..15726)
                     /locus_tag="AC159U_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3336259.1"
                     /translation="MRKLSKFSIFILITILFGLISVPVDSSANELLSLSELVKNLKPS
                     VVNISTTNVVKRRSSPFGGSPFGSPFEGQEDPFEDFFEKFFEGAPQQEFKQSGLGSGF
                     IISEDGFIVTNNHVVEKANDIKIILESGKSYKAEIIGKDPKTDLALLKIEPEEDLPAI
                     SFGRSDILDIGDWVVAIGNPFGLGHTVTAGIVSAKGRSLGLGSYDDFIQTDAAINPGN
                     SGGPLFNLDGEVVGVNTAIFARAQGIGFAIPASLAVNVIDQLKNSGKVVRGWLGVLVQ
                     QITPEIAESIGLSEIKGALIADITPGGPADKAGIKRGDVITEFNNITISEMSELPKSV
                     AITPPGTKSEIELIRDGRRKKVSVVLGELPEQIARKKPEVQEQKDVEDDLGLVVQDIN
                     PQLAKRLGIEQTGGVIITNVYRGSPAWDAGLRKNDIIIELNKKEIDDINQYKKIMNRV
                     NENDSLLFLVKRNQGTIYIALKINGNDN"
     gene            15950..16237
                     /gene="groES"
                     /locus_tag="AC159U_04045"
     CDS             15950..16237
                     /gene="groES"
                     /locus_tag="AC159U_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3336260.1"
                     /translation="MKVQPLYDKILVKRIEGEEKTKGGIIIPETAQEKPLEGTVEAVG
                     KGKLLDNGTIRPLDVKKGEKVLFNKYGGNDINIEGEEYLILREEDILAVIS"
BASE COUNT         5240 a         2891 c         3328 g         4920 t
ORIGIN      
        1 atattagata actaaaacgt ctgtaggata aataataata aattgataga ccagatacta
       61 ttagacctaa aagcagttga aaagattgta aatctatctc tcaaggaaga tataggcaga
      121 ggcgatatta ctaccaattc aattgtaaat gacaagctca aatgtgtagc tgttataaag
      181 tcaaaagaca gaggagtagt tgccggaaac ccagtagcgg agcttgtatt taaaaagctc
      241 gatcccaaaa caaaatacat cccgataaag aaggatggcg atgcaataaa acctggtgat
      301 ataatcgctg agatcagagg ctctgttagg gcggtgttaa caggtgaaag gctggctctt
      361 aacttactgc agcgactctc aggtatagct acattatcaa gcagatttgt agaaaaaaca
      421 aaaggaactg gagttaaaat tctggacaca cggaagacaa cgccagggct tagaatactt
      481 cagaaatatg cagttacagt aggaggctgt tataaccaca ggtttggact ctttgatgca
      541 gtacttataa agaacaacca tataactata gccggagaca ttgaaaaagc tgttgaaaaa
      601 gcgagggcaa aaaataaaaa taaaacaatc gaagtggaaa cgagtaattt tagagaggtt
      661 cgtgaagcat taaaatccgg ggttgatata attatgctgg ataatatgtc acctgataaa
      721 atcaaaaaag cacttgaaat aatagatggg aaagctaaaa cagaagcatc gggtgggatc
      781 aatattaata atataaagca atatgccaag accggtgtgg actatatttc agtgggagca
      841 cttactcatt ctgcaatatc actcgatatt gggttgtatg ttctagagta gttgttatat
      901 tacaattaat ggtacaagag atggaaaata aagccgatat tattgccgaa atagacagac
      961 tcagaaaaga aaaaaacgcc gtaatccttg cacataacta ccaaataccg gatgtacagg
     1021 atatagcgga ctttgtaggc gattcactgg gtctttctca gcaggctggc aggacagatg
     1081 ctgatattat agtattttgt ggtgtgcatt ttatggcaga aactgcttct attatctctc
     1141 cggacaaaaa ggtattgata cccgatctgg gagcaggatg ctcactgtct gatactatta
     1201 ctgctgatca acttagggac tggaaaaagc agcatcccgg agctgtagtg gtatcatatg
     1261 ttaataccac ggctgaagtg aagtctgaaa gtgactactg ctgtacatct tcaaatgcag
     1321 tagaagtagt aaattccatt ccagaagata aagaaatact gtttttaccc gatatgttcc
     1381 tcggcgcttt tgttgcaagg gagacaggaa gagaaattaa tatctggcct ggcgagtgtc
     1441 atgtgcacgc ggacattaag ggtgaggata taaaacgaac cactgatgaa catccggatg
     1501 ctgagcttgt tattcatccc gaatgtggtt gctcgacttc tacgctttac gaatgttcaa
     1561 atgatacatt taacggtaaa cctgtaaaag tactctcaac aggcggaatg ctaaaacacg
     1621 ctaaaagttc aaaatccaaa gaatttataa ttgcaacaga aaccggaatc cttcatacac
     1681 tcaaaaaaca caatcctgaa aaacagtttt atgcagctaa cgaaaatgct gtgtgtaaat
     1741 acatgaagat gataaatctt cataatgttc tggaatcact tcagaatgaa caatttgagg
     1801 ttaaggtgcc ccttccaatt gcaaataagg ctaagaatgc aatagacagg atgctcgata
     1861 tttctgcaca atatcagtaa cctttagtaa gtccaccggc ccttcttgga tcggaatagc
     1921 cataatagta gtctccaatt ttcataatgc tctgcgtgct acccatagct cttccaactt
     1981 ttatttcatg gcctttcttt tctaatatct tattggtgtc attgctaaaa ccgctttcca
     2041 taaacaaaat atcaggaagc cactgatgat gaaatcttgg tgatgcagta gccgttgcta
     2101 tattcatttt atgatctatg acgtttgata taatatgaag tactgtagta attattctgc
     2161 ttccacccgg gctacctgtt gcaagaaaag gctttttatc ttttagcaca atagtcgggc
     2221 tcatagaact tagcattcgt ttttccggtt ctatagcatt aaactctcca ccaacaagtc
     2281 cgtatgcatt aggcacaccg ggtttagctg aaaaatcatc catttcatta ttcagcaata
     2341 ttcccgttcc tttagccatg attttagaac cataactaaa gttaattgta tatgtgtttg
     2401 ataccacatt tcccgatttg tctataactg tgaaatgagt cgtgtcatta ccaccagcaa
     2461 tatacgggtt gccgggccct atatttttac ttggagtaga tttattcagg ttaatttttt
     2521 tgctgagata ttttgcgtat ttttttgaag ttaactcgct gattggaacc tgtacaaaat
     2581 ccgaatcacc caggtatttt gatctgtccg cataggctaa cttcatggcc tcggctatta
     2641 tatgaacaga ccttgagtta ttatgaccaa tattggatat atcaaagtgt tctagcatat
     2701 taagtatctg tataagatga atcccccctg agcttggcgg aggcatggag tagatttcat
     2761 aacctctata tgttccataa attggttttc ttattaccgg tttatatgag cttaggtcat
     2821 ctgctgtgat tagcccatcg ttttcattca tatccgaaac aatcttttca gctatctttc
     2881 cattataaaa tgcatccggt ccatgtttat taatctgttt cagactccat gctaaatcct
     2941 tttgtactat tttctcacca attttgtact attttctcac caatatcgta tgaatctcca
     3001 tcttctttga aaaaagtgtt ataggcatca ggcgattttt tcatcctttc tttaactgac
     3061 ttaagtgaac tactgagctc gtagttaaca tccagaccat tcttcgcata tttaattgct
     3121 ggttttaatg cttttttaag agggatagtt ccgtatttct caagcgctaa agacagacct
     3181 gcaactgttc cgggaactcc tgcagccaaa tatgaatgtc tggatttttc tttatcgaca
     3241 tttccatctt tgtccaaaaa catatccctt gttacagctt taggggcctt ttccctatag
     3301 tcaatggcaa tgacttccct tgaatctgca agatagataa gcataaatcc gccaccacca
     3361 aggtttcctg cccttggata agtagccgcc attgtaaagc caatagtaac agcagcatcg
     3421 atagcattgc cgccatcttt taaaatctta agcccggctt cagtagcctc cttgttatca
     3481 gacgctacca ttccgttttg agcaatcaca ggctggaaaa ttgcattaga ggttataatt
     3541 gggtctgata taagttgatt attaaacaac gttataccga ttagaaataa aaatatttga
     3601 agctttatat tcataattat tatcatatat acttaaatgt atttttaaaa taataatgtt
     3661 ttgaaatatg gagagtaaat aatgtcagaa aaaataaata aaacagagca agaatggcaa
     3721 aaggagctaa ccccggagca gtatagagtt actagagaaa agggaaccga aagaccattt
     3781 accggaaagt acaacgagaa taaggaaaag ggtaagtatg tatgcgtgtg ctgtggaaca
     3841 gagctttttt catctgatac aaagtttgac tcaggctccg gctggccaag cttttataag
     3901 ccagaaaaag aggaaaatgt taaagcggag acagatagga gtcacgggat gataagaaca
     3961 gaggtaatgt gcgataaatg tgatgcccat ctgggccacg tttttgagga cgggccaaac
     4021 cccacgggct taagatactg tataaattcc tgctctttgg attttaaaaa agaagattaa
     4081 caattaaggt tctgaaaaaa gagttagcac gcctgtatat gtgaacagat gattgcttcc
     4141 catcattggg gcaatctccg aatttccaaa aaatccaatc attggaatat cggggaagta
     4201 gcttgatatg taagccgtgt cgatgtcttt gtttccgtaa aaatatgatc ccctcccgca
     4261 gcaattaaaa tatattccga atcttacttt actcatatca gcaatttgtg atttaagatt
     4321 ttcaagcata tccttaagat cttctcgcgc catctcgggg tttcttaatg ttatgactat
     4381 ttcatcacca acattgatat cttctgcaat ccctattatg ccagaattat catctacgcc
     4441 cataagattt ctaaccatat aatttcttgt gctgtccgta tcgctcttaa ccgctgagca
     4501 gagccccacg cttacaattc ttaaaagatc atttggattt tctaatatat gtgccgggac
     4561 gtatgccttg agtacttcaa atgccggtct gccgtctatt tccattatca cgttgcggtt
     4621 acattctgta actctaagcg gtttagatgc gggtttacag ccctgagtca gaccaatatt
     4681 ataattaaag ttaccgctaa ttatcgcccc tgaaaggctt gaactgttaa ccatattatc
     4741 tctatactgg aaagttgcag gtgaactagg gtcttcagaa catgtagcgc caattatatg
     4801 tactatatca cttgcgtgtt caagtgagtt aaagaataag ttatgatgga aactaaaagg
     4861 atcggcaagg accatagcca gtttggtttt agtgttaatc tctataagtc tgttgataat
     4921 attattgcca gatgcttggc ccatatccgt agaaattgaa tcaataaaac actttgcttt
     4981 aagactatct gaacacactc cgagaactac tatcccaggc tcaccctcta tctcaccaaa
     5041 atgagataat actcctgtgg cgctgcaccc tgtgatattg tgtgttcctg ttatttcaga
     5101 tactttttct aatattttct ggtaattatt tctgtggtgg aatgtagtga atattaaaat
     5161 aaaatcggct ttttcgattc cagcctgagt taaacaatcc ctacaagcat tctcagcagc
     5221 ctctaaactt tttggattat ttgaacatcc tacgccggct ttaatcaatt ttataatata
     5281 atacttatgt tggaaaacgg ataactaata tacattgttt taagctattg ttagatgtat
     5341 taatatctca attaataata tattatggta ccttgaaata tacattttgt tctttataat
     5401 acctgctttt gtactttaaa cttggaaagt gtagagatgg agtcagcaaa gatatttagt
     5461 gggaattcaa acatacctct tggcaacgct atttgcgagt atctgggagt ccgtcccggt
     5521 gaaatagata ttcatagatt tagtgacggg gagatattta taaatatcaa agagagcata
     5581 aggggagacc acgtatttat tatccagtcc acatgtcctc cggtaaatga gcacataatg
     5641 gagcttctaa ttattacaga tgccctcaaa cgtgcttcat caaaggaaat aacggctgtt
     5701 attccatact tcggctatgc cagacaggat agaaaagtac aacccagagc accgataagc
     5761 gctaagcttg tagcggaaat actgcttaga gccggcgttg acaggcttgt tactgtcgat
     5821 ttacatgccg ggcagattca gggctttttt gatgtgcctg ttgatcatat atatgctgcg
     5881 ccaatattca tagattatat aaagaaaaga tttgatggag aggatatagt aatagtatcc
     5941 cctgatgccg gaggtatgga gagggcaaga gcatatgcaa aaagactgca tgctacccta
     6001 gccatgaccg ataagagaag aacggctcca aatgtagcag agatcatgta tcttattggt
     6061 gatgtaaagg ataaaactgc cataatagtt gatgatatgg ttgacactgc cggcactgcc
     6121 gtacaggcag caaaatctat aatggccgag ggagcaaaga gtgttagtct ttgctgtact
     6181 catggagtat tatcaggaaa ggctgttcag aggataaacg attcacattt tgaagaggta
     6241 ataataacag atactatccc gccaagagaa gaaacgctga aatgcgagaa gattaaattt
     6301 ttgagcattg ctgatctgct tggtgaagca ataaaaagaa taacaaccgg agaatctata
     6361 agtacattat ttaattaaat tttaggagtt taaaaatggc acaaagcgta ttaaatgtaa
     6421 agagaagagt aaggatgggg aaaagcggct ctaaggctat aagaaaagaa ggaaatatac
     6481 ccgcggttct atatggaaaa ggttcagagc ccgaatcgat agtggtaaat cctgctgagc
     6541 ttaaagaagc actttcaact gatgctggtg agaatacact tcttgaaatg catattagcg
     6601 attgggaagg gggaaatgag actacaaagc tatccctgct tagagacgtc caaatggact
     6661 acttaaaaaa taaatcaatc catctcgatt ttcttgagct ggacatgaaa cagacttttt
     6721 ctgttaagat tccggtaaaa atcaagggaa taccaattgg ggttcgtgaa gaaaagggaa
     6781 tgcttgaaga gatagtgaga gaggtagaga ttgagtgttt gccttcggat attccaaatg
     6841 aatttgttat agatgtgacg gatctgcata taactgaatc tattcatgta aaggacttag
     6901 aggtttcaga taaggttacg attttagatg atccaggctc aacaattgtt acagtacttg
     6961 ctccaagggt tgaaaaaact ttgactgcag aagaagaagg tgaaggtgaa gaggctacgg
     7021 aaacagagag agaagagaaa aatgaggaat ctgcagggtc tgaataaatt attatataaa
     7081 caatagtgga tgtttctaat agcaggtctg ggtaacccgg gcagagagta tgaaaacaca
     7141 cgacataacg tggggttttt gggtgtagat agaattgctc aagatatagg ggtgagtgtt
     7201 acaaaaaagg ggtttcaaag tttttatgaa ttagggcatt tcggaaacca aaaagtttta
     7261 cttattaaac cccaaaccta tatgaacaat agcggaaacg ctattagaga agcgaaagag
     7321 tattataaaa tagatacaga aaatttgata ataatacatg atgagatgga tataccactt
     7381 ggaagaatta aaattaaaaa tggtggaggg agtgccggac acaatggaat taaatctata
     7441 atcgcaaata taggctctca gaattttcca agggtaagga taggggtagg aaaaccttac
     7501 gacagggata aggtaattaa gcatgttcta tcaaatttta ataaagaaga aagtaaacag
     7561 ctttttgaag ttttagatag cgtaagagac agtgtttatg aaattattac aagtggtatt
     7621 gaaaaagcca tgaatcgatt taatataaga gccgaaaaaa atatagaaaa tgaaattgaa
     7681 aaagaggaag tttaaaaatt aaatgatttt taagttttcg ttatattaag gaggaagtaa
     7741 gttatgagtg agttaatcgc ttcgcttgga gttttagttt ctttaggagt tttgggttac
     7801 gcatacatag tgtatggcgg ccttaaagca cttcctgaag gaaacgataa gatgaaagaa
     7861 attgcaactg ctattcacga gggtgcaatg gtatttctta agagtgaata taaaattata
     7921 ggtatttttg ccgttattat atttttccta gtatggtttg ggcttggctt tgctacagca
     7981 cttgcatatg catgcggcgc gggcctttcc tgccttgcgg ggttttttgg aatgatgtcc
     8041 gcaacaaagg caaacgtaag aaccgcacag gctgctgcaa gcgatggaca gggagcagca
     8101 ctttctgctg catttaaagg tggaaacatc atgggtcttt ctgttgcagg acttgggcta
     8161 cttggtcttt cagttggata tgcgcttcaa ataggtattc ctgaaaatcc aaccggtaca
     8221 gcactcactg gatttgttac caacttttcc ggagtaatag gcggttttgc catgggcgcc
     8281 agttcggttg ctctttttgc aagaatcggc ggcggtattt ttacaaaagc agcagacgtt
     8341 ggtgctgacc ttgtaggtaa ggttgaagca ggaattcctg aagatgatcc aagaaacccg
     8401 gcaactattg ctgataacgt tggtgataac gtcggagacg tagcaggtat gggtgccgac
     8461 ctttttgagt catatgtaag ttctgttatt gcgaccatag tgatagcagc tggtatgaca
     8521 gatcataata gaattgctct tatggcaatt ccaatcgttc taattacaat cggcagtgta
     8581 gcttcaatac ttggagcaag atatattgaa agaaagaaag atgatgaacc tcaatcggtt
     8641 cttagatatg gaacattctt tgccggcggt atttaccttg gagtaagttt acttgttctt
     8701 ggactgcttg gcctggttga cggcattggg gtgtttgcag ttaaagtctg gggtgcagca
     8761 gtatttggcc tgctctcagg tgtagcaatt ggtcttgtaa ctgaatatta tacatccgga
     8821 gctcccgtta aaaagatagc cgaagcatca caaacaagtg tagccacaaa tataattgaa
     8881 ggtcttgcag tcggtatgaa aagtacagca tggccagtgc ttataatcgt gcttgccatt
     8941 agttgttcat tttggtttgc aggtctttat ggaatcggaa ttgcagcagt cagtatgtta
     9001 gcaacaatcg gtatcacaat gtcagttgat gcttatggac ctgtggctga taacgctggt
     9061 ggtataacag aaatgtctga gcttggtgat gacgtaagag taatcacaga taagcttgat
     9121 tcacttggaa acacaacagc tgctataggt aaaggatttg caatcggctc agctgcactt
     9181 gctgctcttg ctctttttgc agcatacaca aaggctgttg gtcttgattc aattaacctt
     9241 acaaacagca aggtagtagc agggatgctt ataggtggaa ccattcctta cttcataggc
     9301 gctcttacaa tgggttcagt aggaagaacc gctcagcaga tggttgaaga agttagaaga
     9361 cagtttagag aaatcaaagg gcttatggaa ggtgaagcaa aggctgatta tgcaaaatgt
     9421 gttgagatta gcacaaagtc ctcaatcaaa gaaatgattt ggcctggagt tacagcagtt
     9481 tcagctccta tcataatagg ctggatactt gggcctgaag cacttggcgg atttttagca
     9541 ggtgctctag tttcaggtgt tatgcttgct cttatgatgg caaatgccgg tggagcatgg
     9601 gacaatgcta agaaatatat tgaagagggc aatctcggag gtaaaggatc agatgctcat
     9661 aaggcagcag ttgtgggtga cacgattggt gatccattta aagatacaac aggacctgca
     9721 atgaacattt tgattaaact tatgtcaatt gtttcacttg ttgtagctcc tgctcttttt
     9781 atgtaatata aataaaaaat aaaataaaaa gttctgtcac tctgggtcta tattaagacc
     9841 cagggtcgat attgaccaag acggaggtta agatgcaaag ttatgaaaca gtttttataa
     9901 cacctgtaga tattgcagaa gaaaatctga ataagatttt agataagatc aaagaagcag
     9961 tttccaaaaa tgatggggaa ttatcaaaac tggaaaaatg gcagcaaaga gagcttgcct
    10021 accctataaa cgatctcacc aaagggatct attatattgc agagtataag gctaactctg
    10081 atgtagtaca tgacattgag cacaatttta agtttttaag agaagatatc ctgcggtttt
    10141 taaccataaa aaccaagcct gctaaggcaa aaccagtaaa acccgaagct tcaaaaccac
    10201 aagctacatc agcaccagca tcagctgctc caaaacccga ggcttctgcg ccagtaactg
    10261 aaactcctga ggctactgca acacctgctc caatcagtgc tgagcctgct gctgcatcaa
    10321 cagatacgga caacagtgcg tttgcaagcc caaattctaa ttcaaatgat aaaggaggac
    10381 agagctgatg gaatcaccaa gagatagaag atatatgaat agaaagaaat actgcaggtt
    10441 ttgtaagaat aaagaagaga ttgagtataa aaatgtagat ctgcttgcaa ggtatattac
    10501 cgagcgaaga aaaatcgttc cacgcagggt taccggagtc tgtgctaaac atcaaagaat
    10561 tcttgcaaca gctatcaaaa gagcaagggt actggcttta atcccattta ctgttttaca
    10621 caaataatag ccgcatattg gtattggagg taaaaaatga aagttatatt aactactgac
    10681 gtagactctg ttgggtatat gggagaaata aaagacgtta aaaggggtct tgcaagaaac
    10741 tacttactac ccaaaaatct tgctgtagaa gcaacaccag gaaaccttaa aatttggaat
    10801 caaaaacaaa gaattattga aaaacatcag ataaaaagaa aagaagacgc cgagagtctt
    10861 gcaagcaacc tgggttcagt aacgtgcaca ataaaagtaa aggtcggtga agagggcaaa
    10921 ttgtttggct ctgtaaccac ccagaacata tccgatgatt tagcacaaaa gggttataaa
    10981 atatccaaaa aagatatcgt tctcgatgcg ccaattaaag agacaggcac ctatgatgta
    11041 ccggttaaaa ttcaccccga agtttcagca tcagtaaaag ttgttgttga aaatgaagaa
    11101 ggtgagcagt cttctgagac tggcgatcaa agtgaggaac aggcaactga gcaagctgct
    11161 gaaagttctg agcaggaaaa ctcccttgaa agttcagagg aagcttcagc tgacgaaaat
    11221 agttctgagc aatctgagac taaagagcag gaaagctgat cttttataat attttcggta
    11281 ctaaatttta ttcttcttgt aatctttagg aggtctgaaa tctttatcgg ctaattcgac
    11341 ctcttttatt gacaatgtag tttcaaccct gtttccggga agattgtaat caacagtttc
    11401 catgggatac ccgattttat aaatctcaga aagggtttta ctaaacctgt tttgattttc
    11461 aggtcccagg taaagacctt gtgaaatttt ttgaatttca ctataaaatt cagcaagctt
    11521 attaaaatct atttccccca gtttattgct tacccacaaa tcctgtatta cttttccatc
    11581 ttcaagtagt tcaaatttct cagtttttag acctgcaatt tcttttgttt gatctgtttt
    11641 attgattttc cagtttttta atttttcatc accgttaata tttattcgtt ttttctccaa
    11701 tattttaagt tgttgaactc ttttagcttt atccatgttt gcaagatctt tctttaatgc
    11761 ctttttttgc tcggtcatgt ttgcttgcac aagctcaaca tatttatcag gtgagctttc
    11821 aacatactgt tttgattgtt ggtcaataat ataaagcttg tttttgttga gattgaagat
    11881 gataattggg tcatcattat ctgcaacaaa cttgatttta ttgtctgaaa tgtatatttg
    11941 cccctttcct tttttatcgg gatttttttc cagataactt tcctgctcta gtaaaagtcc
    12001 tgctatagta ggagtattta ttaaaagtga taaaaagagt gcaaatataa aaagtataaa
    12061 attccttaaa agtgaatcca tcttctcctc catattaact aaaaaagata attaaagctg
    12121 cctttgaggt attatagcgt ataatttacg ttttaatagt acagattata ttgacggcta
    12181 tactaataat ggtagattta aactctttat aaacagatca catggaggct taccaaatgg
    12241 ctgaaatcaa tacaaaattt gcaccggttg gcgaaaaaga agttacaaaa gcgattgtag
    12301 agggatttgc taaggaattt actgaacata ttgagagtga tgtcattgtt gtaggagccg
    12361 gcccaagtgg tcttgtagct gcaaaagata ttgctaaaaa gaaatacaaa gttttgcttg
    12421 tagaaagaat gaactatttg ggtggaggtt tttggatcgg tggttattta atgaacaaag
    12481 taaccgtaag agatccgggc cagactgtac ttgatgaaat agaagtacct tatgaagaag
    12541 taagtgaagg actatatgtt gctgacggtc cttatgcctg ctctaaactc attgcaacta
    12601 catgtgaagc aggggcaaag attcgtaata tgaccatgtt tgatgatctt gtgtacagag
    12661 aaaatggcag agtagcggga atagtcatga actggacacc tatagcaaac atgccaaaag
    12721 aaatcacttg tttagaccct atcgcaatcg aatcaaagct tgtgatagat gctactggtc
    12781 acgatgcata tcttgcatca agactttctc aacagggact ttataaaaaa ctaccgggac
    12841 acggctcaat gtgggtagaa aaatcagaag aagcacttgt tgaatatacg ggtgaggttc
    12901 atcctggcct gattgcatgt ggtatgtctg ttaacaccat atatggtctt ccaaggatgg
    12961 gacctacatt tggcggaatg cttctatcag ggaaaaaagc agcagaggta gcagtaaaca
    13021 tcttacagga gcaggccgga ggttaagaat acatccagcg gcattttgtt aggttaaatt
    13081 tcctataagc tgcctatgat gcttgcacag ctgtccatta tactgtattt ccagcacatc
    13141 cttttgccaa tgtgagttaa acaggcagca tgtcttgttt tcacaaaagg gttttcctgt
    13201 aagctcatac atgatcggtt gtaaagtgta ggactgtaat atttcggtag tcttaggatc
    13261 gttgtattca agatactgac ctttaaagta ctcatcgagc tcttttaagt ctcttccatg
    13321 ttgtaaaaac tgggctttta agaaatagta ctccctaggt cttgcaggag cctcaacgag
    13381 tcccgaagtc gatatgaatg acggatgtcc catgagtaca actcttgcat gatatcttct
    13441 gttttcaaac gtgcagagca gtttatctgt ataaattatg tgtattacag aatcggaaat
    13501 atttgagtct aataaaaaat atttattaat tacacttata tatagatatc catcataaag
    13561 aattttattg tttgtatttt ggttttgaga tgcgtggcat aattcgagct tctctttttc
    13621 aatatcatcg atgagggtta gtgaaagata atgatttaac tcattttgct tagcagtact
    13681 taatcgcaga tattcaaaga agtctccttt atattcagac tctataccga atttagataa
    13741 atgttctatt atattggaaa tattagtagc tttggaagga gtatctgaat ataaataaac
    13801 cggtctttta ttcatttata gtgggaagag aggtgttttt aaatctcggc tattaaatca
    13861 atctctacac aactttgcat cggaagattt gatacaccga cagcagctct tgaatgaaca
    13921 ccaatatcgc caaatacatc gaataacagt tcagatgctc cgttaataac ctttggctga
    13981 tcaaaaaaat caggactgga cattacataa ccatttagtt ttacaatctt ctttattcta
    14041 tccagattat caattattgt tgatatagca gataatgcat taattgcagc tagtcttgca
    14101 gcttcgtatc cttgttcaat ggttatttca tcaccaactc ggcctttata tattaaactt
    14161 ccattttgta aaggaagctg acctgaaaca tatatatgat ttccacttat taaggccggg
    14221 gtatagctgc caagaggtat aaccggcttt ggaatttgta tgtttaattc aacaagtcgg
    14281 tcagaaatat tagttgtcat tgccatttat ttttaaagct atatatattg tgccttgatt
    14341 tcttttaacc aaaaacaata aagaatcatt ctcatttact ctgttcataa tctttttgta
    14401 ctggttaata tcatcaatct cttttttatt aagctctata ataatatcat tctttctaag
    14461 cccggcatcc caggcagggc ttccacgata tacatttgta attattactc cccctgtttg
    14521 ttcaatgccc agacgttttg caagttgtgg gtttatgtcc tgtactacta gtcccagatc
    14581 gtcttctacg tccttttgct cttgtacctc aggctttttt cttgcaattt gttctggcag
    14641 ctcgccaagc acaacactaa ctttttttct tctgccatct ctaataagct cgatttctga
    14701 tttggttcca ggaggcgtta ttgctacaga tttaggaagt tcagacattt cgctaatagt
    14761 tatattatta aactcggtaa ttacatcacc tcttttaatt ccagccttgt ctgcagggcc
    14821 tccgggagta atatctgcta taagcgcacc ctttatctcc gataggccta tgctttctgc
    14881 aatttcaggg gtgatttgct gtacaagcac acccagccat ccccttacca ctttcccgct
    14941 gttttttagc tgatctatta catttactgc aagagatgcc ggaatagcaa aaccaatccc
    15001 ctgtgcgcga gcaaaaattg ctgtgtttac acccactact tctccgtcaa gattaaaaag
    15061 aggtccacca ctgtttccgg ggttaatggc cgcatctgtc tgtataaaat catcatatga
    15121 gccaagacca agagacctac ccttagcgct tacaatacct gctgttaccg tatgtccaag
    15181 accaaacggg tttcctattg ccactaccca gtccccaata tccagtatat ccgacctgcc
    15241 aaaagaaatc gctggtaaat cttcttcagg ctctattttc aacagtgcta gatcagtttt
    15301 agggtcttta cctataattt ctgccttata gctttttccg ctttccagta ttatctttat
    15361 atcgtttgct ttctcaacaa cgtggttatt agtaacaata aaaccatctt cgcttatgat
    15421 aaaccctgat cccaggccgc tttgcttaaa ttcctgctga ggggcaccct cgaaaaactt
    15481 ttcaaaaaaa tcttcaaatg gatcttcctg tccttcaaac ggagaaccga aagggcttcc
    15541 gccaaatggg gaagatctcc tttttactac atttgtagtg cttatattca ctactgaggg
    15601 ttttagattt tttactagct ctgagagtga tagaagctca tttgcgctag agtctacagg
    15661 tacagaaatt aaaccaaaaa gtatggttat taatataaaa atactgaatt tactaagttt
    15721 tctcatttaa ttataatctc ctggtaaaaa agtaatatta tttattgaac tatacaatgt
    15781 caagaatgga tacgatgcta aataaattaa tagattaaga aaatattaaa taaaatcatt
    15841 agcttaagcc ttgacaacta ttataacatg attatcttaa agggcgtttt ttagcactta
    15901 agaactaaca gtgctaatta ttataataca aggaggggta aatttaggta tgaaagtaca
    15961 accattatat gacaaaattt tagtcaaaag gattgaaggt gaagaaaaaa caaagggtgg
    16021 cataataatt ccagagactg cacaggaaaa acctctggaa ggtacagtag aagcagttgg
    16081 aaagggcaaa ttacttgata atggcacaat cagaccacta gacgttaaaa aaggcgaaaa
    16141 agtgctattt aacaaatacg gtggaaatga tatcaacatt gaaggtgaag agtatcttat
    16201 tttaagagaa gaggacatct tggcagtaat aagttaagta aataacaaag ctaaaaaaat
    16261 taggaggcaa taatcaacat gggagcaaaa gagataaaat ttaacagaga agcccaggac
    16321 ctgatactgc aaggtgtaaa tgaacttgca aacgcagtaa aatccaccct tgggccaag
//
LOCUS       DBNGSV010000114         3627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_782552, whole genome shotgun sequence.
ACCESSION   DBNGSV010000114 DBNGSV010000000
VERSION     DBNGSV010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_782552"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="AC159U_04050"
     CDS             complement(<1..1568)
                     /locus_tag="AC159U_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3336261.1"
                     /translation="MFSLKISEISLKDAGKAIARLNSKDMTDLGLSSWDLIEIEGKRK
                     TVTRVMPLDKSENNKSIIKIDGITRENAKASVDDHVFVKKVDSRIAIKAVLTPVSPEI
                     IGTDKEAKFILNRLDGMPITVGDRIRVNLSGAKPEDFNVIATVPAESVILRSTTKLEI
                     KKRPKHKVDPICVTFEDVGGLGEQIKRVREIVELPLRYPQLFEKLGIDPPRGLLLVGP
                     PGCGKTLLAKAVAQETNTNFQVINGPEIIHKFYGESEARLRDIFEVATRNQPSIIFID
                     EIDAIARKRDKVTGDVEKRVVSQLLALMDGLKDRGQIIVLAATNLPNLLDPALRRPGR
                     FDREVALEVPDRNGRHDILQIHTRGMPLDTDVDLDKIADLTHGYVGADLENLCKESAI
                     IALRKVLPEVDLENGPSNHQQLFRLKVSNNNFLEALKEIEPSAIREIFVEVPKITWDD
                     VGGLDEVKDLLRESIMWPIKYSEVFDSTNTRVPKGILLHGPPGTGKTLVVKAIANESG
                     INFISVKGSEVLSK"
     gene            1665..2780
                     /gene="recF"
                     /locus_tag="AC159U_04055"
     CDS             1665..2780
                     /gene="recF"
                     /locus_tag="AC159U_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3336262.1"
                     /translation="MQLSANKNKVLVKTLTLLNYRNYINQSFSFADKINLIIGKNGHG
                     KTNLLEAINLVCKFKPFKKTNFDELINFEKNETRLKAELLNNSNLNEINIQLLKGRKI
                     IKLNNKILYRTARYSTLNKLVTFLPSDIDLIKGQASVRRNYIDQMITGIDSEHMADSK
                     NYYQILKQRNALLAKSPNSSAMLEIWDEKLSEPAERIVFRRMKFIKKISPLINKYYEK
                     ISATNSKVDMVYKSTIDITPNFKNDFREDLQKRMSKDVLRKFTTSGPHRDHIIFRING
                     EDSSKFASQGESKSLVLSIKSTEIELYEQTSGEHPILILDDVSSELDDMRKNYFSLLL
                     NNYSGQVFVTTANLEPAFKKNADRIIHIQSGRVLKVD"
     gene            2809..3261
                     /locus_tag="AC159U_04060"
     CDS             2809..3261
                     /locus_tag="AC159U_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3336263.1"
                     /translation="MSLQNKISEEIKEALRGQQKLKLSVLRMLQASIKNKIIDTGQKE
                     LSDEQIITVVNSEIKKRRDSVSEFEKVGRQDAVQAEKDEIAILMEYMPEQLSEEQIVS
                     LINESVSELNIESIKDLGRLMKALMPRVKGKADGNLVNRLVREKLDNL"
     gene            3303..>3627
                     /locus_tag="AC159U_04065"
     CDS             3303..>3627
                     /locus_tag="AC159U_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336264.1"
                     /translation="MQEIIDNQTATDTTNWDLTDLYKDLEDPQIKVDVDQITNDAKAF
                     EKNYRNKIDSDDLDAETLYGAVNELESLSERTGKLLSFAYLMFAGDTNDPKTGAFLQQ
                     MQETAT"
BASE COUNT         1216 a          719 c          657 g         1035 t
ORIGIN      
        1 aatttagaca agacttcaga gcctttaact gaaatgaaat ttatcccgct ttcattggct
       61 atggccttaa ccacaagtgt ctttcctgtg cccggcgggc cgtggagcaa aatacccttg
      121 ggtactcttg tgtttgtact gtcaaacacc tcgctgtact ttataggcca catgattgat
      181 tctcttagta aatcctttac ttcatcaaga ccgcccacat cgtcccaggt gattttggga
      241 acttctacaa atatttctct tatagcagaa ggttcgatct ccttaagggc ttctaaaaag
      301 ttgttattac taaccttaag cctgaaaagc tgctggtgat tgcttggacc attttcaaga
      361 tctacttccg gaagaacctt tctaagagca attatggcag actccttgca gagattctcc
      421 agatcggccc ctacgtaacc gtgagtgagg tcagcaatct tgtcgagatc aacatctgta
      481 tcaagcggca taccacgcgt gtgaatctgc aaaatatcat gcctgccgtt tctgtccggc
      541 acttccagtg ccacttctct gtcaaacctg ccaggtcttc taagagccgg gtctaagaga
      601 tttggaaggt ttgtagcagc aagaactata atctgacctc ggtctttaag accgtccatt
      661 agagctaaaa gctgtgatac cactcttttt tctacgtctc cggttacctt atccctcttt
      721 cttgcaatgg cgtcaatttc atcaataaat ataatcgaag gctggtttct tgtggcaact
      781 tcaaaaatgt ctcttagcct tgcttcactc tcaccataaa atttgtggat tatctcggga
      841 ccgtttataa cctgaaagtt cgtattggtt tcctgagcta cggcctttgc caacagcgtt
      901 tttccacatc ccggaggacc cacaagcaaa agccctcttg gaggatcaat accaagtttt
      961 tcaaataact gcgggtatct gagcggaagc tccacaatct ctcttactct tttaatctgt
     1021 tcaccaagcc ctcctacatc ttcaaatgta acacagatgg gatcgacctt gtgctttggt
     1081 ctctttttta tctctagctt cgtggttgat ctcaatatta cagattctgc aggtacagtt
     1141 gctattacat taaaatcctc tggttttgca ccacttagat ttactcttat tctgtcgcct
     1201 actgtaatgg gcatcccgtc cagtctgtta aggataaatt tagcttcttt atcggtacct
     1261 ataatttcag ggcttactgg agtaagcacc gcttttattg caatccttga atctaccttt
     1321 ttaacaaaca cgtgatcatc tacggaggct ttggcatttt ctcttgtaat accatcaatt
     1381 ttaattatgg acttattatt ctcagactta tccaaaggca taactctggt tactgttttt
     1441 cgtttgcctt ctatctcaat aaggtcccac gaggaaagcc ccagatcggt catgtccttg
     1501 gagtttaacc ttgcgatcgc ctttccggca tcctttaatg atatttctga tattttaaga
     1561 gaaaacatag tttatctaat ggaaatatta ctgctctctt taacccggtc aagcactaca
     1621 aaattgaaga gctatatata taatttataa tctgcaaaaa acaattgcaa ctatccgcaa
     1681 ataaaaacaa agtgctcgtt aaaactctta cactgctaaa ttatagaaac tacataaacc
     1741 agtcattttc atttgctgat aaaataaatt taatcatcgg taaaaacggc cacggcaaga
     1801 ctaacctgtt agaagctatt aatttagtct gcaagtttaa gccttttaaa aaaacaaatt
     1861 ttgatgagct tataaatttt gaaaaaaatg aaacccggct aaaagcagaa ttgctaaaca
     1921 atagtaatct aaatgaaata aacatccagc ttctaaaggg cagaaagatt ataaagctaa
     1981 ataacaagat tctatacaga accgcaaggt attccacact caataaactc gtaacatttt
     2041 taccatcaga tattgacctc ataaaagggc aggcatctgt gaggagaaac tatatcgatc
     2101 agatgataac aggcatagac tctgaacaca tggctgattc aaaaaactac taccagattc
     2161 tcaaacaaag aaacgcactg ctggcaaagt ctccaaacag ttctgccatg cttgaaatat
     2221 gggatgaaaa gctctcagag cctgccgaga gaattgtatt tagaagaatg aagtttataa
     2281 agaaaatctc acctttgata aataagtatt atgagaaaat atctgccaca aattcaaagg
     2341 ttgatatggt ttacaaatct acaatagata tcactccaaa ctttaaaaat gatttcagag
     2401 aagacctgca aaagagaatg agcaaggatg ttttaagaaa atttactact tccggaccgc
     2461 acagagatca tataatattt agaataaacg gagaagactc aagcaagttt gcttcccagg
     2521 gtgaatccaa aagccttgta ctttctatca aatcaacaga aattgagctc tacgaacaaa
     2581 cctcaggaga acaccccata cttattcttg atgatgtttc aagtgaactg gatgatatga
     2641 ggaaaaatta tttttctctt ctattaaaca attatagtgg acaagtattt gtaactacgg
     2701 caaatttaga acctgcgttt aaaaaaaatg cagatagaat aattcatata caatcgggca
     2761 gagttttaaa agtagattaa aaacaataat tttaatgcat ataagaaaat gagcttacaa
     2821 aataaaatat cagaagaaat aaaagaagca cttagaggac agcagaaatt gaagctctct
     2881 gttttaagga tgcttcaggc ttcaattaag aataagataa ttgatacagg gcaaaaagag
     2941 ctatccgatg aacagataat aacagtagtg aactctgaga ttaagaaaag aagagattct
     3001 gtctcagagt ttgaaaaagt tggaaggcaa gatgctgtgc aggcagaaaa agatgaaata
     3061 gccatactca tggagtatat gccggagcaa ttatctgaag aacagatagt aagccttata
     3121 aatgaatcgg tttctgagct taacatagaa agcattaagg atcttggcag actcatgaaa
     3181 gccctaatgc caagagtaaa aggaaaagcc gacggcaatc ttgtaaacag gctagtaaga
     3241 gaaaaactgg acaatctgta ataatatatc taaatctaaa aagtaattat aagaggataa
     3301 acatgcagga gattattgat aatcaaacag caacagatac cacaaattgg gatttaactg
     3361 atctttataa ggatctggaa gacccgcaga taaaagtaga tgttgaccaa atcacaaatg
     3421 acgcaaaggc atttgaaaaa aattacagaa ataaaatcga cagtgatgac ctggacgctg
     3481 agacacttta cggagccgta aatgagcttg aatcgctcag tgaaagaaca ggcaagctgc
     3541 tatcctttgc ttatcttatg tttgctggtg atacgaatga tcccaaaacc ggagcgttcc
     3601 ttcagcaaat gcaggaaacc gctaccg
//
LOCUS       DBNGSV010000115         3527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_574697, whole genome shotgun sequence.
ACCESSION   DBNGSV010000115 DBNGSV010000000
VERSION     DBNGSV010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_574697"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..859)
                     /gene="panB"
                     /locus_tag="AC159U_04070"
     CDS             complement(50..859)
                     /gene="panB"
                     /locus_tag="AC159U_04070"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3336265.1"
                     /translation="MNKIRVPDIIKKKKDKQKLVMLTSYDAITARIVDEAGVDIILVG
                     DSLGNVVQGQDTTIPVTIEDMIYHTRLVSRGAKTAHICADMPFMSYQTSKEQALQNTG
                     RLMKEGFAESVKLETTDEYIDTIKSIVNAGIPVMGHIGLCPQSFHNMGGYKIQGRDKK
                     EKNRYIKLAKATESAGAYSIVLEGIPLELAKAITNSVSIPTIGIGAGPHCDGQVLVIN
                     DLVGLHSSRVPKFVKQYADFKGLMTKAIKSFSSDVTKSKFPAKDNSFSWSN"
     gene            complement(1118..1813)
                     /locus_tag="AC159U_04075"
     CDS             complement(1118..1813)
                     /locus_tag="AC159U_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3336266.1"
                     /translation="MKKEFLLICMLIFAWSCGVSKYYPKRKPKPRPPVEQELPGLGDL
                     EGEVSGNVQVGLASWYGIEEHKRRAASGEVFDKDELTAAHKALPMDSIVRVTNLENGM
                     DVIVRINDRGPFVAGRVIDLSYAAAKEVDLIGPGTAQVKVEVISTPNREDNYFKGKYV
                     VQVGSFKDKSNAYKLKKKLSANFDNVNVHEYSHNNDSYYRIRLVGYDTKSSAEAEYKK
                     LKNLGYKPRIKLE"
     gene            1995..3179
                     /locus_tag="AC159U_04080"
     CDS             1995..3179
                     /locus_tag="AC159U_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3336267.1"
                     /translation="MDKNSEDFSLIQRLPNYVFNSVNELKTKYRAKGVDIIDLGMGNP
                     DQPTPKHIVDKLVESVTKPRNHRYSASAGIPKLRLAICDWYKRNYNVDLNPDTEAVAT
                     IGSKEGISHLMLSILSPRDMVIVPNPAYPIHIYSVIIARGDIQGVKLSDDSTLIDDIE
                     KKVKEVYPRPKVLSISFPNNPTTQTVDLDFFKRIYELAKEYGLIVVHDFAYADLCFDG
                     YKAPSFLQVKGAKKVGVEFYSLSKSYSMPGWRVGFMVGNPKIIAALKRIKSYMDYGIF
                     QPIQIAAISALNGPQNCVAEITEIYRSRRDRLCEGLTRAGWHIEKPKATMFVWGEIPE
                     QFKKMGSLEFSKLLIKKAGVAVSPGIGFGNHGEGYVRFALVENEQRIMQAVRGIRKLI
                     NS"
     gene            3305..>3527
                     /locus_tag="AC159U_04085"
     CDS             3305..>3527
                     /locus_tag="AC159U_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3336268.1"
                     /translation="MTTKSQEESPRDRIIKTALDLFYSQGYLATGINQIISESGVSKN
                     TFYYHFPSKEDLCVAYLRARHDIWSGWLKD"
BASE COUNT         1074 a          681 c          623 g         1149 t
ORIGIN      
        1 attcggaaaa ggctttcata tctgcaatat tgtctatcag ttccatattt taattactcc
       61 agctaaagct gttatctttt gcagggaatt tggattttgt tacatctgaa gaaaaagact
      121 ttatggcttt tgtcattaag cctttaaaat ctgcatactg ttttacaaat ttaggaactc
      181 tggaactatg cagtccaaca aggtcgttta ttacaagcac ctgaccatca caatgcggcc
      241 ctgcaccgat tcctatcgtg ggaattgata cgctgtttgt tattgctttt gcaagctcaa
      301 gaggtattcc ttccagtact atgctgtagg caccagcaga ttcagtagcc ttggcaagct
      361 ttatatatct attcttttct tttttatccc tgccctgaat cttgtaacct cccatattgt
      421 gaaaggactg cgggcagagg ccgatatgac ccattacagg aattcctgca tttactatgg
      481 attttattgt gtctatatac tcatctgtag tttcgagttt tacagattcg gcaaatcctt
      541 ctttcattaa ccgcccggta ttttgtagtg cttgttcctt tgacgtttga taagacataa
      601 acggcatatc tgcacagatg tgagctgttt ttgcgccgcg tgataccagc cttgtatgat
      661 agatcatatc ctctatagtt acaggaatag tagtgtcctg accttgtact acatttccga
      721 gggaatctcc aaccaggatg atatctaccc ctgcttcatc cacaatacga gcggtaatgg
      781 catcatagga ggtaagcatg acaagctttt gcttatcttt ctttttcttt attatgtcgg
      841 gcactcttat tttattcata attcataatt tattattcat aaaacattag tgcagcaccc
      901 caaaaaagac aaaaaaaagc cagcagagag tttgtctctc agccggcagt cattttatat
      961 tttttattag aggtacggct cattgttccg tcctgatttt tccttttata aaaaaataaa
     1021 tctaattaag ctaatcataa gtctcagttc ctgttttttg gaatctaagc ttagtaaaaa
     1081 tttaattact atatttataa tgtcaaattt ctataactta ctcaagtttt attcttggct
     1141 tataaccaag attttttaat tttttgtatt cagcttctgc actgcttttc gtatcgtatc
     1201 ctaccaacct tatcctgtag taactgtcat tattgtgtga atattcatgt acatttacat
     1261 tgtcaaagtt tgccgaaagt ttctttttta gtttatatgc atttgatttg tctttgaacg
     1321 aacccacctg aacaacatac ttacctttaa agtaattatc ttccctgtta ggagtggaaa
     1381 taacctctac ttttacctgt gctgtaccag gcccaataag atcaacctct tttgcagctg
     1441 cataggataa gtcaataacc ctaccggcaa caaaaggccc cctgtcattt atccttacaa
     1501 taacgtccat tccattctca agatttgtta cccttacaat ggagtccata ggaagcgctt
     1561 tatgggcagc ggtaagttca tccttatcaa atacttcgcc gcttgcagcc cttcttttat
     1621 gttcttctat cccataccaa gaagctagcc caacctgcac attaccactt acttcaccct
     1681 ctaaatctcc caaacccgga agttcctgtt ccaccggagg cctgggtttg ggtttccttt
     1741 ttggatagta cttggaaact ccacaagacc atgcaaaaat cagcatacat atcaataaaa
     1801 attctttttt cattgcagta aattcattga acagacttaa tcagattggt ctattcaata
     1861 agttttataa aatataacct catatttcaa aactagatac aaattttttt aagaaaatat
     1921 cacataacct tcgctgattt cttgagttta cacaacttta gaatagaata tgaattttaa
     1981 tatttttaaa taaaatggac aaaaattcag aagatttctc attaatacaa agattaccta
     2041 attatgtttt taattcagta aatgaattga aaactaaata cagggcaaag ggagtagata
     2101 ttatcgatct gggtatggga aaccctgatc agcctactcc aaaacatatc gtagataaac
     2161 tggttgaatc tgtaaccaaa ccaagaaatc acagatattc ggcatctgcc ggaattccaa
     2221 aactaaggct tgcaatttgt gactggtata agagaaatta taatgttgat ctgaatcctg
     2281 acacagaggc tgtggctaca atcggctcaa aggaaggcat ttctcacctt atgctttcca
     2341 tactgtcacc aagggatatg gtaatagtac caaacccggc ataccccatt catatttatt
     2401 cggtgatcat agctagggga gatattcagg gagttaaact ttcagacgat tctacattaa
     2461 ttgatgatat agaaaaaaaa gttaaagaag tctatcccag accaaaggtt ctctcaattt
     2521 catttcccaa taatcccaca acacagacag tagacctgga tttctttaaa agaatttatg
     2581 agcttgcaaa agagtatggt cttatagtgg ttcatgactt tgcatatgca gacctttgtt
     2641 tcgacgggta taaggctccg agtttccttc aggtaaaagg agcaaaaaaa gttggtgtag
     2701 agttttactc tctgtcaaaa agttacagta tgccagggtg gagagttgga tttatggttg
     2761 gcaatcctaa aataatagca gcacttaaaa ggattaagag ttatatggac tacggcattt
     2821 ttcagccgat tcagattgca gcaataagcg cacttaacgg tccgcaaaac tgcgtagctg
     2881 agataacaga aatttacaga tccagaagag acaggctttg cgaagggctt acaagggcag
     2941 gctggcatat tgaaaaacca aaggcgacga tgttcgtttg gggtgaaata cccgagcagt
     3001 ttaaaaagat gggttctctt gagttttcta agctattaat aaaaaaagca ggcgtcgcag
     3061 tttccccagg tataggtttt ggaaatcacg gcgaaggtta tgtgcgcttt gctctagtag
     3121 aaaacgagca gaggatcatg caggcagtta ggggtataag gaagcttatt aatagttaag
     3181 ttatctttta ctgctttatt caaaattatt aattatatat tttccatcca catatttatc
     3241 tatttaatta ttccttttaa aaaggcactt gacagacaag tctgtctaca ttattattta
     3301 ttccatgaca actaaatcac aagaagaatc tccaagagac agaataatta agacggctct
     3361 tgatctgttt tatagtcagg gctatcttgc aactggaatt aatcagataa tttcagaatc
     3421 cggcgtatcc aagaatactt tttattatca tttcccctca aaagaggatt tgtgcgtagc
     3481 atatttaaga gcacggcatg atatttggag cggatggctt aaggata
//
LOCUS       DBNGSV010000116        12467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1060394, whole genome shotgun sequence.
ACCESSION   DBNGSV010000116 DBNGSV010000000
VERSION     DBNGSV010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1060394"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC159U_04090"
     CDS             complement(<1..377)
                     /locus_tag="AC159U_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3336269.1"
                     /translation="MNRSIVAIFMIFLLAACGEEQQVAELIRPVKYTEVIQTGGNRSR
                     TFSGIAKAGVEFDLSFKVSGNILYLPVNVGDTVKKRDVIAELDPQDYLLKKQESEATL
                     ISAQAQSRNADADYARIKELYEN"
     gene            complement(374..2734)
                     /locus_tag="AC159U_04095"
     CDS             complement(374..2734)
                     /locus_tag="AC159U_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3336270.1"
                     /translation="MSLFYLLLVRPVFPESGTNVAILFDGPSDFNAVIVENYKNEITA
                     LTDGEFYVNFPPSMIIEADWSVQGVDNVLNTVLYDPRVDIVITPGVLSSINVVRRKQL
                     SKPVLAPWVIDNELTGAPVSEEGTSGVKNLNYLSSASVIKRDFKAFKDIIRFNKFAFL
                     FMPVFPEAIPEIEDIVLTTALSIDSEIVMIPVSEDIDQVIKSIPADVEAVYITPLFLL
                     DDVKFNKLVNGLIERKLPSFSLMGRIEVEKGLLLGLNPKQDVSRQARRIALNVQSILL
                     GQDAGSISIRFPRQQQLTLNMKTARAIGYSPRWEVLTEADLLYEEKEHKDNFITLAQA
                     VNESLRNNLDLLATERSVSAGAAEVWRAISVLLPQSNIAFTASRIDEDRARASMGNQA
                     EKSTTGSVDVNQILFSESAFSNVSVQRNLQKSREYNRDEVRLDIIAATSIAYLNVLRA
                     KIFEKIRKSNLKLSKSNLQLAKIRRDIGVANPSEVYRWESEIALDRIDLVDTQAERKN
                     AEILLNRLLHRPLNEQFSTIDISLDSPYFKVGDERITKYIDNPRSFEIFKDFMVSEGL
                     SISPAIRDLDSQIKAQNTVLRSSKSAFILPDISVGAEANRLFSEGGAGQRGDIEGLPL
                     VSSQDDTDWTIFLQASFPLFRGGAKIAEYKQAREQLFSLRLQRQSVIERVEQDIRSAM
                     NNTGASFPSIKFSKNAADAAHKNLELVTDSYSRGVVSILDLLDAQDASISADLNSATA
                     VYDFFIDLVNLHRAIGRFDFYLDKQENDIWFNRLEKFYNKSLEGQK"
     gene            complement(2782..4281)
                     /locus_tag="AC159U_04100"
     CDS             complement(2782..4281)
                     /locus_tag="AC159U_04100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3336271.1"
                     /translation="MYRFLCSISAVLAILLILSSYNINAEIVGSEVYQQLNSQDKVRV
                     IVVLDSADTRFNDLSQKVAAIDDKKLNVLSKLSSSDFSLVKSWDTINAFSGYVSLQGL
                     YKLSNDTNVIKVDLDLGGKGGLNQSRFVINADDVNEAGFTGQGVTVAVLDTGVDTNGI
                     DLEDDIVGQQCFCENENGSGCCPNGNTAQSGNGSAEDDNGHGTNIAGIITSEGILAPE
                     GIARDAQIVAVKVLDNNNSFSNTSQIISGLEWVQNNRPDVDVINMSLGTSLLFGTVCD
                     DATAANMVLAQVIQNLVNSGVMVFASTQNDGSINSIASPACLSNTLSVGAVFDGDYGS
                     VGVFDCTDQSTSKDKVACFSNANADMDMLAPGSQITSTGINIGISTQSGTSTQSGTSQ
                     ACAHASAAAALLLEADSDLTQSEVRQVLRDTGATVQDNRNGLVFPRIDVLAAINFITG
                     LNIVDQNNSPSSLCSVGGKVTPLQFAAIVLIYLVPVLFIVIRRYYYRKN"
     gene            4775..5488
                     /locus_tag="AC159U_04105"
     CDS             4775..5488
                     /locus_tag="AC159U_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009698598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HIR3336272.1"
                     /translation="MIRAFFDSVRDLLPIILVVAFFQIIVLQQPFPNPLKIFIGLFAT
                     ILGLTFFIQGLNLSLFPVGEELAEAFAKKGSIFWFILFAFALGFGTTIAEPALIAVCT
                     EAAKVMAAAGFIQDTETAQYNYATGLRYTVALSVGFSLILGVIRIIKGWPVHWIIISG
                     YIVIVILTQFAPREIIGIAYDSGGVTTSTITVPLVTALGIGLSTVIKGRNPMIDGFGL
                     IAFASLFPIGFVLLYGLLF"
     gene            5485..6270
                     /locus_tag="AC159U_04110"
     CDS             5485..6270
                     /locus_tag="AC159U_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017017508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIR3336273.1"
                     /translation="MTMFETIIPTFLSTIKDVFPIIALLFFFQYIVLKKPIRNLKKAI
                     IGFTYVTIGLSLFLLGLEKALFPIGEVMAKQLTAPEFIDSALDRTPEWQDYYWVYIFA
                     AVIGFSTTIAEPSLIVVSIKANEMSGGAIGFWGLRLAVAIGVALSIALGAFRIVTGTP
                     LHFYMIAGYIVVIIQTLFCPKWLVPLAYDSGGVTTSTVTVPLVTALGLGLASTVPGRS
                     VLIDGFGLIALASLFPMITVMGYAQLSHFLDKNRNKKSKRKGT"
     gene            6273..6623
                     /locus_tag="AC159U_04115"
     CDS             6273..6623
                     /locus_tag="AC159U_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014231118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3336274.1"
                     /translation="MDLRLIVALVSDDYSEKVIDKAREMGATGASVITSGHGEGLKPH
                     KTFFGLTFEGQIDMILFIVEKHLSRNILEGISEVAGFDSASGSGVAMQLNIEDAVGLK
                     TQLETIESEIEDQL"
     gene            6620..7027
                     /locus_tag="AC159U_04120"
     CDS             6620..7027
                     /locus_tag="AC159U_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3336275.1"
                     /translation="MNTKEKVIKVNEVMTPDVKTIDGLATVAEAIQSMKEKKYGALIV
                     DRRDEHDEYGFITVQGIARHVIENNLSPERVHVYEIMNKPVLSVRPDMNIRYAIRLLE
                     QIDHGRALVIKDGKAVGIVTMYDMVIHYMDSNV"
     gene            7231..7929
                     /locus_tag="AC159U_04125"
     CDS             7231..7929
                     /locus_tag="AC159U_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR3336276.1"
                     /translation="MHNKLYKQIIYLITHPKRNKNKFLYFFVSIVVLLLFIPFLEGEK
                     YTSALLHLLTTIILLTGIYAVSHTRKQIVIASVLGLPWLIANWVSLFVAMKTLGPGAI
                     SWGALFFIYTTTIMFSHVIKGSKVTIETLFGAVSVYLLIGLIYTSLYGLLEILSPGSF
                     NLADSSGNIYPFKVTELIYFSYVTLTTLGYGDIIPVSSYARSLAISEAIIGQLYLTIL
                     VARLVGLHIIYSKS"
     gene            7973..9400
                     /locus_tag="AC159U_04130"
     CDS             7973..9400
                     /locus_tag="AC159U_04130"
                     /inference="COORDINATES: protein motif:HMM:NF017870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HIR3336277.1"
                     /translation="MKKILLVVLLIIIAIAVTPYYFGMKAEKQYHEIVSKIKQSPNVT
                     VKKKSYERGFFNSNAKTILEIKSKSKSIILNSEDNITHGPFDIMGFTKGKINLTPFVA
                     TVDSDATIKIPVTTDSGETKTIAMNSEGNTDISFDGTAESSYIIPKYKIIKQSENLVF
                     NWSGVEMNFIYDYKSNNLNSSFLSKSLDITDPDTTFKISNITAKSTTSDIKQNLQSLT
                     GDFEMKIKSIKFASADEKIDMSNLSFSGFSKTSSGKLNMGMKVIGDKLITSEYEFGPL
                     TYDIAIKNIDAAAWEKIQDSANKMKVADSPMEQGMQVMGVLPELVKHSPVIEIKQLSL
                     KSSLGDLNADARIKVKGDNPEMLQNILLIGNTLEAEASIVASKSLVDFYIEKTSTNQD
                     IEIETDNNNVSEGADKLSEGQVFLEGEMKDSIESSDNSSGIKSFIDNGYLIPDGKNYS
                     FKASLKQGQVTINGKPVPLAQMMLQ"
     gene            complement(9413..10273)
                     /locus_tag="AC159U_04135"
     CDS             complement(9413..10273)
                     /locus_tag="AC159U_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3336278.1"
                     /translation="MRKTKRLRQMIEGDEILVLPGVYDCLSAKIAQTTGFGAVFTSGF
                     GISAVRLGLPDYGLITASEMMESVKNIVSSVSIPVVADMDTGYGNPINVIRTVEQALA
                     AGVSGIILEDQQWPKKCGHMEGKKVLSCSEHVQKIKAARQTAGKDLVIVARTDSRAEL
                     GLDEAIKRGHEYKDAGADVIFIEAPRSIDEMKEIVKSFAGTPLFANMVEGGKTPILRA
                     DELNLMGFKIVVYPVSGLFAAVNSVKSCFKYLKDNKTTIGYENDYSFGDFEKLIDLSK
                     YKDIEKNYIS"
     gene            10353..10910
                     /locus_tag="AC159U_04140"
     CDS             10353..10910
                     /locus_tag="AC159U_04140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HIR3336279.1"
                     /translation="MENLNFVPKQAFVTKGVGKHKHKLTSFEMALRNAKIAEYNLVRV
                     SSIFPPDCKLIPTASGLKQMNPGQVVFCVMSDNATNEPNRLIASSVGIAIPKDSSRYG
                     YLSEHHSFGENDKVAGEYAEDLAADMLATILDAPFDPDKSYDEQKDIWKISGHIVKTR
                     NITQSAVGDKKGLWTTVVAAIVFVL"
     gene            complement(10925..>12467)
                     /gene="recN"
                     /locus_tag="AC159U_04145"
     CDS             complement(10925..>12467)
                     /gene="recN"
                     /locus_tag="AC159U_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3336280.1"
                     /translation="IIIDALNLLRGAKASSEFIKTGCTEAQIEGVFDVNAEEIKEYLV
                     EQGIDFDDDLIIKRIISESGKNRIFINGSRITLNVLSAITANLIDIFGQHENIKMLDH
                     ENHLSYLDSFDVDLNLKDKVSDLFKQYQKIETTLRDLNNRQNDKNEREEYLKFVCKEI
                     EDASLKYDEEAELENEKKVLLNHEQLKKTAESSYESLYEADSSIVSKLQNINNEFVKA
                     SEIDDNLREPADLLEKSLVLLQDTSYFLRDYIQSVDSNPDRIDEIENRLQLIYELKRK
                     YGLSIKDVLQKKDDYQLELDSLLNLSSEIEELEDKKEQTFTVLKGLCNDLSHKRRNVS
                     VKMSARIEAGLREVGLKDCTFAIKFDDKPVSSDGIDRVEFYFSANPDEQSKPLAKVAS
                     GGELSRIMLILKTIMNKSDGQTVLIFDEPDTGIGGAVAESVGAKIRKLSDNHQVLCVT
                     HLAQVAKFADSHITVKKEFIDNSTKIKIDLLDKEKRIDELARMMGGVKITQKTIDAAR
                     EMLRH"
BASE COUNT         3826 a         2519 c         2144 g         3978 t
ORIGIN      
        1 ttgttttcat aaagctcttt tattcttgca taatcggcat cggcatttct tgactgggcc
       61 tgtgcgctta taagagtagc ctctgattcc tgttttttta atagataatc ctgcgggtcc
      121 agttctgcta ttacatctct tttttttact gtatccccta catttacagg caggtataaa
      181 atgttgccgc taaccttaaa actcaaatca aattctactc ctgcctttgc tattccggaa
      241 aatgtcctgc ttctgttccc acctgtttgt ataacttctg tatattttac aggtcttata
      301 agctcggcta cttgttgttc ctccccacag gctgcaagca gaaaaatcat aaatattgca
      361 acaatacttc tattcatttt tgtccctcta atgatttatt gtaaaatttt tcaagacgat
      421 taaaccatat atcattttct tgtttatcca gataaaaatc aaaccgccca attgctctgt
      481 gcaggttaac aagatcaatg aaaaagtcat atacagccgt cgcggaatta agatcagcag
      541 aaatagatgc atcctgagca tctagcagat ccaatatgga aactacaccc ctggaataag
      601 aatccgtaac aagctcaaga tttttatgtg cagcatcggc agcattttta gaaaatttaa
      661 tactcggaaa ggatgctccg gtgttattca tagctgatct gatgtcctgc tctactcttt
      721 caataacaga ctgtctctgt agtcttaagc taaagagttg ctcacgtgct tgcttatatt
      781 ctgcaatctt ggctcctcct ctaaaaagcg gaaatgaagc ctgcaaaaag atcgtccaat
      841 cagtatcatc ctggcttgat accagcggaa gaccctctat atcacctctc tgccctgcac
      901 caccttcaga aaatagccgg tttgcttcag cgcccacact tatatcgggg agtataaaag
      961 cactctttga agatctgagc actgtgtttt gtgctttaat ctgagaatcc aaatctctta
     1021 tcgcagggga tatgctaagc ccctcgctta ccataaaatc tttaaatatc tcaaacgatc
     1081 tggggttatc aatatatttt gttattcttt catcccctac tttgaaataa ggagaatcca
     1141 gtgatatatc tatagtagag aattgttcat ttagtggtct gtggagtagt ctgtttaata
     1201 aaatctccgc attttttctt tcagcctgcg tatctacaag gtctatcctg tcaagcgcga
     1261 tttcactttc ccatctgtac acttctgatg ggtttgcaac tccaatatct cttctaattt
     1321 ttgcaagctg caggtttgat tttgaaagct tgaggtttga ttttcttatt ttttcaaata
     1381 tttttgctct tagtacattc agataagcta tggaagttgc agctatgata tcaagcctaa
     1441 cctcatcgcg gttatattcc ctggacttct gtaaatttct ttgaacactc acattggaaa
     1501 atgcagattc tgaaaacaat atctgattaa catctacaga accagttgta cttttttcag
     1561 cctgatttcc catacttgca cgagccctgt cttcatcaat cctgctggca gtaaaagcta
     1621 tatttgactg aggcagcagt actgaaattg ctctccatac ctcggctgcg ccagcactta
     1681 ctgaacgctc ggttgctaga agatcaaggt tgttccttaa tgactcattt acagcctggg
     1741 caagcgttat aaaattatct ttatgctctt tttcttcata taataaatca gcctccgtaa
     1801 gaacttccca ccggggtgaa taaccaattg ctcttgctgt tttcatatta agagtaagct
     1861 gctgctgtct tggaaatctt attgaaatgc ttcctgcatc ctggcctagc aggatacttt
     1921 gtacattcag tgcaattctt cttgcctgcc tggaaacatc ttgtttggga tttaaaccaa
     1981 gaagcagccc cttttctacc tctatccttc ccataaggga aaaagatggc agttttcttt
     2041 ctattaatcc atttacaagc ttgttaaact taacatcatc cagaagaaaa agcggagtaa
     2101 tataaacagc ctccacatct gcaggaatac tttttattac ctgatctata tcttcactca
     2161 cagggatcat gactatttct gaatctatac tgagtgcggt agtaagaact atatcctcaa
     2221 tctcggggat tgcttcggga aaaacaggca taaacaagaa tgcaaactta ttaaatctga
     2281 ttatatcttt aaatgcctta aaatctcttt tgataactga tgcagaggac aggtaattta
     2341 ggtttttaac cccacttgta ccttcttcac tcacaggagc acctgtaagc tcattatcaa
     2401 taacccatgg cgctaaaaca ggcttactaa gctgttttct tctaactacg tttattgatg
     2461 ataaaactcc gggagttatg acaatgtcaa ccctagggtc ataaagcaca gtgtttaaaa
     2521 cattatcaac cccttgcact gaccagtctg cctctattat catagaagga ggaaaattca
     2581 cataaaactc accatcagta agcgcagtaa tctcattttt atagttttct acgattaccg
     2641 cattaaaatc tgacggcccg tcaaataaaa tcgcgacatt agttcccgat tcaggaaata
     2701 caggtctgac gagcaggaga tagaacagac tcaataagca tacgagttta tagtgatggg
     2761 ggctgagtga catttaagta tttagttttt tctataataa tatctcctaa ttacaataaa
     2821 taaaacagga acgagataga taagcacaat tgcagcaaat tgcaatggtg tcactttacc
     2881 cccaacagag cataatgatg aaggagaatt gttctgatcg acaatattaa gtcctgtaat
     2941 aaagttaatc gcagcaagca cgtctattct tggaaagaca agcccatttc tgttgtcctg
     3001 caccgtagcg cctgtatcgc gtaagacctg ccttacctct gattgagtaa ggtcagagtc
     3061 agcttctaag agcagggcag ctgcagccga tgcatgagcg cacgcctgtg aggttccgct
     3121 ctgtgtagag gttccgctct gtgtagatat tccaatgtta atacctgtag atgttatttg
     3181 tgagcctgga gctagcatat ccatatcggc attagcattt gaaaaacatg caactttatc
     3241 tttactcgta ctttgatcag tacaatcaaa tacacctaca ctcccgtaat ccccatcaaa
     3301 cacagcacct acgcttagtg tattactaag acatgcaggg gatgcaatac tgttaataga
     3361 cccatcattc tgagtagatg caaataccat cactcctgaa tttaccaaat tttgtataac
     3421 ctgtgccaat accatattag cagcagttgc gtcatcacaa accgtgccaa agagaagtga
     3481 agtaccaaga ctcatattta tcacatccac atccggcctg ttgttctgta cccattccaa
     3541 accagatatt atctgagaag tattcgaaaa gctgttgttg ttatcaagca ccttaaccgc
     3601 aacaatttgg gcatcacggg cgattccctc cggagctaga ataccttctg atgtaataat
     3661 gccggctatg ttagttccat gaccattatc atcttcagca ctgccgtttc cgctctgtgc
     3721 agtattgcca tttgggcagc atccactgcc attttcattt tcacaaaaac actgctgccc
     3781 cacaatatca tcctctaaat caatcccatt tgtatctact cccgtatcaa gcacggctac
     3841 cgtaacaccc tgacctgtaa aacctgcttc gttaacatca tcggcattta ttacaaatct
     3901 gctctggttg agaccacctt tgcctcccag gtctaaatct actttaataa cattagtatc
     3961 attagagagc ttataaagac cctgcaagga tacatacccc gaaaatgcat taattgtatc
     4021 ccagctcttt actagcgaaa aatcactgga acttaacttt gaaagaacat taagcttctt
     4081 atcatcgatg gcagcaacct tttgactcag gtcattgaac cttgtatcag ccgaatcaag
     4141 aactacaatc acccgcactt tatcctggga attcagttgt tgatagactt cacttcctac
     4201 aatttctgca tttatattat aagaagataa aatcaacaag atagccaaga ctgcagaaat
     4261 actgcataag aatctataca tctattctga cctcccaata taaatgaaat aatatttaag
     4321 tatgctaaat taataatata caatagattt ttatttataa ataatagttc gaacaaagtg
     4381 tttagcaaat tatttgatca tataatctac ttaattaccc tttcaaaaag aaataagaat
     4441 aaatttttgt attttcttgt ttatatcatg ctgcctttat tatttatcct gtttatagag
     4501 agaaagatca tctacaattt cacttagggc gtattaaata aaatttaatg gccaaagtta
     4561 aaccaatttc aaatttcata cataaaggta gatttttgca ctaatatgct tccaatttga
     4621 gatcataaaa atcgtaaagg gaatcattat gccctgaaac atattattga taataaatac
     4681 tgagaataga agtatttgca ttaattatta attactttag aatccttgtt agtattaaat
     4741 aattatcatc aatgataggg ataacaccta aaacatgatt cgcgcttttt tcgacagtgt
     4801 gagggaccta ctaccgatta ttctagtagt cgcttttttt caaatcatcg tactacagca
     4861 accttttcca aaccctttaa aaatttttat cggcttgttt gctacaattt tggggctaac
     4921 attttttatc cagggcctaa atttgagttt gttccctgtt ggagaggaat tggcagaagc
     4981 atttgcaaaa aaaggaagca tattttggtt tattttgttt gcattcgcct tgggattcgg
     5041 aacaacgatt gcagaaccag ccttgatcgc agtttgtact gaagcagcaa aagttatggc
     5101 ggccgctggt ttcatacaag ataccgaaac agcacaatat aactacgcta ccggacttcg
     5161 gtatacagtc gctctatctg ttggattttc tcttattctt ggcgtcatca gaattataaa
     5221 ggggtggccg gttcactgga tcattatatc aggctatatt gtaattgtta ttctgacaca
     5281 attcgccccg cgtgaaatta ttggcattgc ttatgattca ggcggcgtca caacatcaac
     5341 aattacagtg ccgcttgtga cagcgctcgg aattggatta tcaactgtta tcaaagggcg
     5401 caatcccatg attgacggat ttggactcat tgcgtttgcc agtctttttc ctatcggttt
     5461 tgttttactt tatggcttgt tattctgact atgtttgaaa caattattcc aacattcctg
     5521 tcgactataa aagacgtttt cccaattata gccttattgt tcttttttca gtatattgtt
     5581 cttaagaagc caatcagaaa ccttaaaaaa gcgattatag gattcacata cgtcacaata
     5641 ggactgtcac tgtttcttct gggactggaa aaagcccttt tccctatcgg tgaggtaatg
     5701 gccaaacaac tcacggcacc ggaatttatt gactctgctt tagaccggac accggaatgg
     5761 caggattatt actgggtata tatttttgcg gcagtcatag gattctcaac aacaattgcc
     5821 gaaccctctc ttattgtcgt ttccatcaag gcaaatgaaa tgtcgggagg cgctatcggc
     5881 ttctggggcc tgcggctcgc agttgccata ggtgttgccc ttagcattgc cttgggtgct
     5941 tttcgtattg ttacaggcac ccccttacat ttttatatga ttgccggata tatcgttgtt
     6001 atcattcaga ctttgttttg ccctaaatgg cttgttcctc tggcctatga ttcaggtggt
     6061 gtaacaacat caaccgtaac tgtacctcta gtcacagctt tagggttggg gctggcctct
     6121 actgttcctg ggcggagcgt gcttattgat ggatttggcc tcatagctct tgcgagccta
     6181 tttcccatga taacagttat ggggtatgct caattgtcac actttttaga caaaaatcgc
     6241 aataaaaaat ctaaaaggaa agggacttaa taatggactt aagactaatc gtcgcccttg
     6301 tttcagatga ctattcagaa aaagtcattg ataaagcccg cgaaatggga gctactggtg
     6361 catcagttat aaccagtgga catggagaag gcttaaaacc tcataaaact ttctttggtc
     6421 tcacatttga aggtcaaatc gacatgatat tgtttattgt cgaaaaacat ttaagtcgta
     6481 acatactgga aggaatctcg gaagtagcag gttttgatag cgcttcaggc tcaggcgtcg
     6541 ctatgcagtt aaacatcgaa gatgccgtag gtttaaaaac gcaattagaa acaattgaat
     6601 ctgaaatcga ggatcagtta tgaacaccaa agaaaaagtt ataaaagtta atgaagttat
     6661 gaccccagat gttaaaacaa ttgatggtct ggcaaccgtt gcagaggcca ttcaaagtat
     6721 gaaggaaaaa aaatatggcg ctctaattgt tgatagacgc gatgagcacg atgaatacgg
     6781 ttttatcacg gtacaaggta tagcaaggca tgtaattgaa aataatcttt ctcctgagcg
     6841 cgtgcatgtg tatgaaataa tgaataaacc tgttttaagc gtgaggcctg acatgaatat
     6901 acgttatgcc ataagacttc tcgaacagat tgatcatgga agagctttgg taattaaaga
     6961 tggcaaggca gtaggcattg ttaccatgta cgatatggtc atccattata tggatagcaa
     7021 tgtttaatga atgtcaaacc ccggaattta ggtccacttt cagagttaga atttattaaa
     7081 attttatcag taaattgata taaatgcttg ttcatattct tggttttacc tttattacat
     7141 cccaataata agaatgaaat gtatttagtt tgtataataa ataatatatt atgattttta
     7201 ttaacataac gattaataat tacaaaccaa atgcataaca aactttataa acaaataatc
     7261 tatttaatca cccaccccaa aagaaacaag aataaatttc tatatttttt cgtatccatt
     7321 gtcgtgcttt tgttatttat cccattttta gagggtgaaa agtacacctc tgcactgcta
     7381 cacttactta ctactataat tttattaact ggcatttatg cagttagtca cacaagaaaa
     7441 cagattgtta tcgcttcagt gttaggacta ccgtggttaa ttgcaaactg ggtcagcctt
     7501 tttgttgcaa tgaagaccct tggacctgga gcaataagct ggggagcact ttttttcatt
     7561 tatacaacaa caataatgtt ttctcatgtg attaagggtt ccaaggttac aatagaaaca
     7621 ctgttcggag ccgttagtgt ttatctatta atagggctta tatatacaag tttatacggc
     7681 ttattggaga tactatctcc cggttctttt aatctggctg attcatcggg caacatttat
     7741 ccatttaaag ttacagagct catttatttt agttatgtta ctctaactac actcggttat
     7801 ggtgatatta ttcccgtaag ctcttatgcc aggtcacttg cgatatcaga ggccataatc
     7861 ggacagttat atctcacaat acttgttgca agacttgtcg gacttcatat catctattca
     7921 aaatcgtaat aaataatcta taataacatt aactcaatgt aggggagttc aaatgaaaaa
     7981 aatattatta gttgttctac taatcataat agcaattgca gttacgcctt attacttcgg
     8041 aatgaaagct gaaaagcagt atcacgaaat tgtatcaaaa attaaacaat caccaaatgt
     8101 aacagtaaaa aagaaaagtt atgagagggg attttttaat tctaatgcaa aaacaattct
     8161 tgaaatcaaa agtaagtcaa agagcataat tctaaactct gaagataata ttactcacgg
     8221 accttttgat atcatgggat ttacaaaagg taaaataaac ttaactccat ttgttgctac
     8281 agtagattca gatgcaacaa taaaaatacc tgtaaccact gacagtggag aaacgaaaac
     8341 tatagcaatg aactctgaag gaaacactga tattagtttt gatggaacag ctgagagcag
     8401 ctacataatt ccaaaataca agataatcaa gcagagtgaa aatcttgtat ttaactggag
     8461 tggtgttgaa atgaatttca tctatgatta taagtccaac aacttaaaca gctcatttct
     8521 atccaaatca ctggatatca cagaccctga tacgacattt aaaatttcta acattacagc
     8581 caaatcaacc acgagtgata taaaacaaaa tctgcaaagc cttaccggag attttgagat
     8641 gaaaataaaa tccattaagt tcgcatctgc agatgaaaaa atagatatga gcaatctaag
     8701 tttttccggt ttttccaaaa ccagcagtgg taagcttaat atgggcatga aagttatagg
     8761 cgataaactc atcacaagtg agtatgaatt tggcccactt acttacgata tcgctataaa
     8821 aaatatagat gccgcagcgt gggaaaaaat tcaggattct gcaaataaaa tgaaagtagc
     8881 cgacagtcct atggagcagg gaatgcaggt gatgggcgtt ttgccagaac ttgtcaagca
     8941 ttcccctgtt atagaaataa agcagcttag tcttaaaagc agcctcggtg acttaaatgc
     9001 ggatgcaaga ataaaagtaa aaggcgataa ccctgaaatg cttcagaaca tactacttat
     9061 aggaaacact cttgaagcag aggcttcaat tgttgcttca aaaagtcttg tagattttta
     9121 catcgaaaag acttcaacca atcaagacat cgaaattgaa actgataaca ataatgtatc
     9181 cgagggtgcc gacaagctat cagaagggca agtattttta gaaggtgaga tgaaagatag
     9241 tatagaaagt tctgataaca gttcaggtat taagagtttt atagataacg gctacctgat
     9301 acctgatggt aaaaattata gttttaaagc ctctcttaaa caaggtcagg taactattaa
     9361 tggaaaaccg gttccacttg cacaaatgat gcttcaataa atgattcaaa cattaagata
     9421 tataattctt ttctatatcc ttatatttag aaagatctat taatttttca aaatcaccaa
     9481 agctgtagtc attttcatat ccgatagtag tcttattatc ctttaaatat ttaaagcaag
     9541 attttacaga atttaccgct gcaaaaagcc ccgatacagg gtatacaact attttaaatc
     9601 ccatgaggtt aagctcgtct gctctgagaa taggtgtctt ccccccttct accatatttg
     9661 caaaaagtgg tgtgcctgca aatgatttta cgatctcctt catctcatca atactccttg
     9721 gagcttcaat gaaaattaca tccgccccgg catccttgta ctcatgccct ctttttatag
     9781 cctcatccag accaagttca gccctgctgt cagtccttgc aacaataacg agatccttgc
     9841 ctgctgtctg ccttgctgcc tttattttct gcacatgctc tgaacaggat aaaacttttt
     9901 taccttccat atgaccgcac ttcttcggcc actgctgatc ttctaaaata atcccagaca
     9961 ctcctgctgc aagcgcctgt tcaactgttc ttattacatt aatcggattt ccatatcccg
    10021 tatccatatc cgctacaacc ggaatactta cgcttgatac gatatttttt acagattcca
    10081 tcatttcaga tgcggttatt aacccatagt caggaagccc cagtcttacg gcagatatac
    10141 caaacccact tgtaaaaact gctccaaaac ctgtagtttg ggcaatcttt gcactaaggc
    10201 aatcatacac cccgggaagc accaggattt catctccctc aatcatctgt cttagtcttt
    10261 tagtttttct cacattgctc tccatataat aataataaat tattgacaat gaaataatta
    10321 tagggattta atataacgct aatttatata ttatggaaaa tttaaatttt gtccccaaac
    10381 aggcgtttgt aacaaaagga gtaggaaagc ataagcataa gcttacaagc tttgaaatgg
    10441 cgcttagaaa tgcaaagata gcagagtata atcttgtaag ggtttcgagc atatttcctc
    10501 ccgattgcaa actcatcccc acagcctccg ggcttaaaca aatgaacccc ggccaggtgg
    10561 tattttgcgt gatgagtgac aacgcaacca atgaaccaaa caggctcatt gcatcttcag
    10621 taggcatagc aattcctaag gacagcagca gatatggata tttatcagaa catcacagtt
    10681 ttggtgaaaa tgataaagtg gcaggtgaat acgctgaaga ccttgccgcg gatatgctag
    10741 ccactattct agatgccccg tttgatccgg ataaaagtta tgacgagcaa aaagatatct
    10801 ggaaaataag cggtcatatt gtaaaaacaa ggaatataac tcagtctgca gttggtgata
    10861 agaaaggtct ttggactaca gttgttgcag caatagtttt tgttttgtga tattgagtat
    10921 tagactaatg ccttagcatt tctcttgcgg catcaatggt tttttgagta atttttactc
    10981 cacccatcat ccttgcaagt tcatcgatcc tcttttcctt atccagtaaa tcgattttta
    11041 tctttgtaga gttatctata aactcttttt ttacagtgat gtgagaatct gcaaactttg
    11101 caacctgagc cagatgcgta acgcaaagaa cctgatgatt atcagataac tttcttattt
    11161 tagctccaac actttctgct accgctcctc caatacctgt atcaggttca tcaaaaataa
    11221 gtactgtttg cccgtcagac ttattcatta ttgtttttaa aataagcatg atcctggata
    11281 gttctccacc agatgctact tttgcaagag gtttactctg ctcatcagga tttgccgaga
    11341 aataaaattc aactctatca atgccatccg aactgactgg tttatcatca aattttattg
    11401 caaatgtaca atccttaagc cctacttccc taagtcctgc ctcgattctt gccgacattt
    11461 ttactgatac atttcgcctc ttatgcgaca ggtcattaca caaaccttta agaaccgtaa
    11521 aagtctgttc ttttttatcc tccaattctt ctatttcaga ggataaattt aatagactat
    11581 ctaattccaa ttgataatca tctttctttt gtaaaacatc ttttatagat agtccatact
    11641 ttcttttaag ttcataaata agctgcagcc tgttttcaat ctcatcaatc ctatcaggat
    11701 tggaatctac agactgtata taatctctta gaaaataaga cgtatcctgc aataaaacca
    11761 gagatttttc caacaaatcg gcaggttctc taaggttatc atctatctcg ctagctttta
    11821 caaattcgtt attgatgttt tgcaatttac ttactatgga gctatctgcc tcatataaag
    11881 actcataact gctctcggct gtttttttta gctgttcatg gtttagtaag accttctttt
    11941 cattttcaag ttcggcctct tcgtcatact ttaatgacgc atcttctatt tctttacata
    12001 caaattttaa atattcttct ctttcatttt tatcattttg tctgttattt aaatcccgca
    12061 gggtagtttc aattttttga tactgtttga ataaatcaga taccttgtct tttagattca
    12121 aatctacatc aaatgaatca agataagata ggtggttttc atgatctagc atttttatgt
    12181 tttcatgctg accaaaaata tctataaggt tagcagttat agcggatagt acgtttaaag
    12241 ttatcctgct gccgtttata aatatgcgat tcttgccgga ttccgaaata atccttttaa
    12301 ttataagatc atcatcgaag tctataccct gctctacaag atactctttt atctcttcag
    12361 catttacgtc aaagacacct tcaatttgag cttcagtaca gccggttttg ataaactctg
    12421 aagatgcctt agcacctctt agtagattga gcgcatctat tatgata
//
LOCUS       DBNGSV010000117         2203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1268283, whole genome shotgun sequence.
ACCESSION   DBNGSV010000117 DBNGSV010000000
VERSION     DBNGSV010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1268283"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            587..1918
                     /gene="ltrA"
                     /locus_tag="AC159U_04150"
     CDS             587..1918
                     /gene="ltrA"
                     /locus_tag="AC159U_04150"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR3336281.1"
                     /translation="MELGELPKGRFDRIPKEFIAERLAESPTGDIRLMEMVCERENMR
                     KALLRVEKNAGAPGVDGMKTHQLRGYMKQHWEEIKASLLDGTYRPSPVRRKEIEKPDG
                     GIRPLGIPTVLDRCIQQAMAQVLQELWDYTFSEHSYGFRPGRSQHMAIRQAKCYVEEG
                     YTHVVDIDLSKFFDRVNHDRLMSRLAQRITDKRMLKLIRAFLNSGILIGDIIEEPGEG
                     TPQGGPLSPLLANIVLDELDKELEKRGLRFVRYADDCVIYVRSKRAGERVMRSITRFI
                     TWRLKLKVNEAKSSVTHPWLSRYLGYRITRMFGATRIGIHQKSIKRFMDKIRVMTARE
                     RGQSINQVISEINGYIRGWWAYFCIGRSGAIIKELNNWLLRRLRAYIWKQWKLPRTKV
                     QELISRGVSRRWAVMIGNTRKGAWRLSKHGQLIHALPERYFTAQLGLVLLG"
     gene            complement(2023..>2203)
                     /locus_tag="AC159U_04155"
     CDS             complement(2023..>2203)
                     /locus_tag="AC159U_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336282.1"
                     /translation="GVDEIKRYCTAVFAAEKDIFTADRMLPAQVRQGQTEKIETRILI
                     IQVIPEYRTFNAEKH"
BASE COUNT          578 a          540 c          683 g          402 t
ORIGIN      
        1 ggctgtttgg cgggcacgct ccgcgagcgt accaaaacca ttcatgctgt caaggatagc
       61 atggaggcgg aggtcgaatc aatgagcaaa tcccgcccaa gccaaaggta acggccaagg
      121 gaagcggaag gcaactgtat ccccgtgagg ggagcggaga ggaagctgga agcgataatg
      181 cgggctgact gtacaaggaa gcggatatga ggcgaggtgc acggggacga gggtgcaaca
      241 caaccggaag tcccgataag ttactcagcg cggcgcacca cgtagatccg acggctacgc
      301 aggaaataac agccagggaa gaccccggga gatccggtat ccgtccgcca gaggcggact
      361 gggcaagccg taaggctcgt cgaacggaaa accggaagtc agcagagggc atagtaagct
      421 acgggaaggc aaggctagtg aggcactccg acgaaagaag gagtaacaga taggccggag
      481 ccgtaaaatc tggggcagaa ggcctgaacg gtgaacggcc cgagatggcc gtcaaatgat
      541 agtgacgaat aggagctgaa gacttatgaa cacagtgcag atgtgcatgg agcttggcga
      601 actgccgaaa ggcagattcg acaggatacc aaaggagttc atagcggaac ggctggccga
      661 aagtccgaca ggagacatcc ggctcatgga gatggtctgt gagcgggaga acatgcgtaa
      721 ggccctgctt agagtggaga agaacgcagg ggctcccgga gttgatggca tgaaaaccca
      781 tcagttgcgc gggtatatga aacagcattg ggaagagatt aaggcttcgc tgctggacgg
      841 cacttacagg ccgtcgccag tacgcaggaa ggagatcgaa aagccggatg gaggcatcag
      901 gccgcttggt atcccgacgg tgctggaccg ctgtatacag caggcgatgg cgcaggtgct
      961 gcaggagctc tgggactata cattctcgga gcacagctac gggtttagac cgggaaggtc
     1021 tcaacacatg gctatccggc aggctaaatg ctacgttgag gaaggctata cccacgttgt
     1081 ggacatcgac ctttcgaagt tcttcgaccg ggtcaaccat gaccggctta tgagccggct
     1141 ggcacagcgg ataacggaca agcggatgct caaactcatc cgtgcatttc ttaacagcgg
     1201 catactcatc ggcgatatca tcgaagagcc cggagaggga accccgcaag gggggccgct
     1261 ctcgccattg ctggccaata tcgtgctcga cgagctcgac aaggagcttg agaagcgcgg
     1321 actacgcttt gtccggtatg ctgatgactg cgtgatatac gtcagaagca aacgggcagg
     1381 agaacgagtg atgcgcagta taacccgctt catcacctgg aggctcaagc tgaaggtcaa
     1441 tgaggcgaaa agcagtgtga cacacccgtg gctgagccgg tatctcggat acaggatcac
     1501 ccggatgttc ggggcaaccc ggataggaat acaccagaag agcatcaagc gattcatgga
     1561 taagatccgt gtcatgactg cccgcgaacg cgggcagagt atcaatcagg taataagcga
     1621 gatcaacggc tacatacggg gatggtgggc ctatttctgt atcggcagat caggcgccat
     1681 cataaaagag ctcaacaact ggctcctgcg caggcttcgc gcctacatat ggaaacagtg
     1741 gaaactgccg agaacaaagg tacaagagct cataagccgc ggtgtcagcc gacgctgggc
     1801 cgtgatgatc ggcaacaccc gcaagggtgc atggcgatta agtaaacatg gccagctgat
     1861 ccacgcactg cctgaaagat acttcactgc acaactcggt cttgttctac ttggatgacg
     1921 acttgtctat aacagccgaa ccgccggata cgtgatccgt acgtccggtg gtgtgggagg
     1981 ggggctcaga gatggggctc cctatcccga tcggctccga tttcaatgtt tctcagcgtt
     2041 gaatgtccgg tactccggaa ttacctgtat tatgagaatt ctagtttcta ttttctcggt
     2101 ctgaccctga cggacctgtg ccggaagcat tcgatccgcc gtgaaaatgt ctttttcggc
     2161 ggcgaagact gcagtgcaat agcgtttaat ttcatccacg cct
//
LOCUS       DBNGSV010000118         6199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1684488, whole genome shotgun sequence.
ACCESSION   DBNGSV010000118 DBNGSV010000000
VERSION     DBNGSV010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1684488"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="AC159U_04160"
     CDS             complement(<1..1634)
                     /locus_tag="AC159U_04160"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HIR3336283.1"
                     /translation="MNVIGKKVKPVHREIFENNGPKALSEWILEQQKLLITDTTFRDA
                     HQSLLATRMRSHDILNIAEATSRIENDVFSFEMWGGATFDVCMRFLKESPWDRLERIR
                     AKMPNAILQMLLRGSNAVGYKNYPDNVVKEFIDQAAQNGIDLFRIFDCFNWIPNMKVS
                     IEQALDTGKIVEPAICYTGDITDPNRTKYSLDYYIKLAREFSDMGAHIIAIKDMAGLC
                     KPFAAELLVKAIKDETGLPVHFHTHATSGNAEATVLKAAEAGVDIVDGAISSLSGMTS
                     QPSLNAIVAAMENSARNTGLDVKGLNKLASYWENVRVFYSPFEGDMKSSNADVYLYEI
                     PGGQYTNLRAQANSLGLGDRWEEVKEMYADVNLLFGDIVKVTPSSKVVGDLALFLVQN
                     DLTTEDVLTQGNKLSFPQSVIEMLMGQLGQSYGGFPEKLLKKVLGGDKPLKIRPGELL
                     KDEDFDSSGKYLQDKYKVSIDDKDLISYLLYPKVFEEFLQHRIDYDDTSVIPTNTFYY
                     GMEPSDEISVEIEEGKTLLIKLFAVGEMESDGRIPLL"
     gene            complement(1635..3146)
                     /locus_tag="AC159U_04165"
     CDS             complement(1635..3146)
                     /locus_tag="AC159U_04165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3336284.1"
                     /translation="MLKKPAIKPFNKILVANRGEISIRIFRACSELGITTVAIYSEED
                     SFSLHRNKADEAYLIGKDKGPVEAYLDIEGIIQLAKDKNVDAIHPGYGFLSENAHFAR
                     ACEEAGISFIGPSPDSIARMGDKAEGRKIAQEVGVPVVPGTEGFVQTQKQAFDFAKKS
                     GFPVMVKAAMGGGGRGIRICRDKKSLIENFKQSRSEAKAAFGDDSVLLEKYIENPKHI
                     EVQILGDKHGKVIHLFERDCSVQRRHQKVVEIAPSLHLSTDLKNRIYDSAVKIAKEVG
                     YFSAGTIEFLVEGENYYFIEMNTRIQVEHTVTEMVTGVDLIQAQIRVAEGYKLSDNKL
                     SIPKSPRINGYAMQCRITTEDPTNNFQPDIGRLTAYRSSGGFGVRLDAASAYVGAVIT
                     PYYDSMLVKMTTWGNSFEQCILKMDRSLREFRIRGVKTNIPFLLNVIRHEIFKDGQCT
                     TTFLDTHKELFDIREPKDRATKLFSYIGDTIINTSLKKPDIWKDKPPALPKVP"
     gene            complement(3246..3641)
                     /locus_tag="AC159U_04170"
     CDS             complement(3246..3641)
                     /locus_tag="AC159U_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3336285.1"
                     /translation="MSEDKELFLAELFGTKPHVHLIGGKCNKCGKYTFPKYVACPHCF
                     SDDIVEAPLSKTGKLHSFTMVRRSMPEFVVPYGLGLVDFPEGVRVMAQIDKDYIEDLE
                     LEADMEVEIGPVRKGKDGKDLHSYKFKPA"
     gene            complement(3674..4825)
                     /locus_tag="AC159U_04175"
     CDS             complement(3674..4825)
                     /locus_tag="AC159U_04175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3336286.1"
                     /translation="MRDVYIIGVGTTSCGRFPDKGAHVLGSDAAWKAILDSGIHARKI
                     EIAFCGHVYQGMGVGQRTFKEIGLVGQPVVNVEGACGSGTLSLWEAWRSIAYGQFDVA
                     LALGVENLSRILSGGPLPLEDDDIEVALGMGMPALYAMRAKRYMAEYGTTEEQFANVV
                     VKSRKHAALNPIAQYRKQISVEEILNSPMIADPLTRNQCCPVGDAAAAAVLCSEDKVK
                     ELATKQPVKILGCISQSGKYNTPDKLICDPSENVARTSSMAYEMAGLGPEDMDLAEIH
                     DAFTVAEMIVVEALGFCKKGEGAELTDSGRTSLGGDSVCVNPSGGLLSRGHPIGATGL
                     LQTAELVWHLRGEAGDRQVPGAKVGIIETMGGAQPAMDGITCVVAILGV"
     gene            5036..6067
                     /locus_tag="AC159U_04180"
     CDS             5036..6067
                     /locus_tag="AC159U_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3336287.1"
                     /translation="MSDLLIDSVPNSSEVKVRDALDAMGIRRSIRWYEPNKPVERWKV
                     QAMLEAARIAPSAGNFNGQRAIVVYRDEDPDIWEFISDWSQITTQMAPILFFWCYDMS
                     GYDVQGQSIHDLMRTGALDKAHGWEYERVNKLFPLPAMLPDFIMHRLAAIDLGNAIQN
                     SMIAASALGLATCLNGASGGARRNVKTKFNLPNTYVFSWLMTVGYPAEKMDGGGTRPR
                     PPFERMFFEKQVGNSFKRDEKTVKLLEELNLIQAPGPLPGRLEEINRLTKRFGLGEEH
                     MTDWKLAPSQTDTDTVTCADTKPVPLTDEEFQATVPGDVPVEMQLTPTVRREVLENYR
                     KEKGIGDAD"
BASE COUNT         1771 a         1323 c         1105 g         2000 t
ORIGIN      
        1 agcagcggaa tcctgccgtc tgattccatc tcacccactg caaatagttt tatcaaaaga
       61 gtctttcctt cttctatctc cacactgatt tcatcactag gctccatgcc gtaataaaat
      121 gtatttgtgg gtattacaga agtatcatca taatcaatcc tgtgctgtag aaattcttca
      181 aaaacctttg ggtaaagcag atatgagata aggtctttat catctattga aactttatac
      241 ttgtcttgca gatattttcc ggatgaatca aaatcttcat cttttaacag ttcaccaggt
      301 ctgattttaa gcggtttatc tccaccaaga actttcttta acaacttttc aggaaaccct
      361 ccataggact gcccaagctg tcccataagc atttcaatta ctgactgagg gaaactgagt
      421 ttgttaccct gagtgagaac atcctctgtg gttaaatcat tttgtactaa aaagagcgct
      481 aggtcaccga ctacttttga agatggcgtt accttaacaa tatctccaaa tagcaggttc
      541 acatcagcat acatctcttt tacttcttcc cacctgtcac ctagcccgag tgaatttgcc
      601 tgtgccctga ggtttgtgta ctgccctccc ggaatttcgt acagatacac atcggcattt
      661 gaggatttca tgtctccttc aaatggagag taaaacaccc ttacgttttc ccagtatgaa
      721 gctagtttat taagtccctt aacatcaagc cctgtattgc gggcagaatt ttccattgca
      781 gccactattg catttaggct tggctgagat gtcattccag atagagagct aatggcgccg
      841 tcaacaatgt ctactcctgc ttctgctgct tttagtacag tagcctctgc atttccactt
      901 gttgcatgag tatggaaatg aactggaagt cctgtctcat cctttatagc ttttacaagc
      961 aactctgctg caaatggctt gcatagaccg gccatgtctt ttatagctat aatatgagca
     1021 cccatatcag aaaattccct agcaagctta atataataat ctaaagaata ttttgttctg
     1081 ttaggatcag taatatctcc tgtataacag attgcgggtt ctacgatttt gccggtgtcc
     1141 agtgcctgct ctattgatac cttcatgttg ggtatccagt taaaacaatc aaatattcta
     1201 aaaagatcga ttccgttttg tgcggcctgg tctataaatt cttttactac attatctgga
     1261 tagttcttat agcctacggc gtttgaaccc ctgagaagca tctgcaaaat agcatttggc
     1321 atttttgccc tgattcgctc aagcctatcc cagggtgatt cctttagaaa tctcatacat
     1381 acatcaaatg ttgcaccacc ccacatctca aacgaaaaca cgtcgttttc aatcctggat
     1441 gtggcctctg cgatgtttaa aatatcgtgt gacctcatgc gggttgcaag aagagactga
     1501 tgagcgtctc taaaagtcgt gtcggttatt aggagttttt gttgttcaag tatccattca
     1561 ctaagggcct tggggccgtt attttcgaat atctccctgt gaactggctt aactttcttg
     1621 ccgataacat ttatttaagg aacttttggt aaagccggag gtttgtcttt ccatatatca
     1681 ggttttttaa gagaagtatt tattatagta tcgccaatat aactaaaaag ctttgttgct
     1741 ctgtccttag gctctcttat atcaaaaagt tctttatggg tgtcgagaaa agtagtcgtg
     1801 cactgtccgt ctttaaatat ttcatgcctt attacattta gtaaaaaagg aatgttggtc
     1861 ttaacacccc ttatcctgaa ctcgcgcaat gacctgtcca tttttaggat acactgctca
     1921 aatgaatttc cccatgtggt catcttaacc agcatagaat cataataagg agtaataacc
     1981 gcaccgacat atgcgcttgc tgcatcgagc cttacgccaa aaccacctga agacctgtaa
     2041 gccgtaagcc ttcctatatc aggttgaaaa ttattagtag gatcttcagt tgttatacgg
     2101 cactgcatgg catagccatt aattcgtgga gatttgggaa ttgacaattt attgtcagag
     2161 agtttatatc cttcggcaac tcttatttgt gcctgaatga ggtctacacc cgttaccatc
     2221 tcggttacag tgtgttctac ctgtattctg gtattcattt caataaagta gtaattttca
     2281 ccctctacca gaaattctat tgttccagcg ctaaagtaac ctacttcttt tgctattttt
     2341 acagcagaat cgtaaatcct attctttaaa tcagttgata aatgtaatga aggagctatt
     2401 tcaactactt tttgatgtct tctttgtaca gagcagtctc tttcaaaaag atgtattact
     2461 tttccatgct tatcaccaag tatctgtacc tcaatatgct tgggattttc aatatatttt
     2521 tcaagtaaaa cagaatcatc cccaaaagca gcttttgctt ctgatcttga ttgtttaaaa
     2581 ttctctataa gtgatttttt atctctgcaa atcctgatgc ctctgccgcc cccgcccatt
     2641 gctgctttta ccatcaccgg aaagccgctt ttctttgcaa aatcaaaggc ttgtttttgt
     2701 gtctgaacaa aaccctctgt tcccggtaca acgggaaccc ctacctcttg agctattttt
     2761 ctgccctcgg ccttatcacc catacgcgct atagaatcag gtgatggacc tataaaactt
     2821 ataccagctt cttcgcaggc cctggcaaaa tgtgcatttt cgctcaggaa gccatatcca
     2881 gggtgaatgg cgtctacatt cttgtccttt gcaagttgta ttattccttc tatatccaga
     2941 tacgcttcta cagggccctt gtcttttcct attaagtaag cttcgtccgc cttgtttctg
     3001 tgcagggaaa aagaatcttc ttctgaatat atagctacag tggttattcc aagctcagag
     3061 catgccctga atattcttat tgatatttcg cctctgttgg caacaaggat tttattaaat
     3121 ggttttatgg caggtttttt aagcatagat ttataagaat taataatttg atataaaaaa
     3181 acctcttttg gactttatat tatccaaaag aggtctgtta atacaacttt tatttttatt
     3241 tttttttatg caggcttaaa tttataagaa tgtagatcct taccgtcttt accttttctt
     3301 acaggaccaa tttctacttc catatcagct tccagctcta ggtcttcgat atagtcctta
     3361 tctatttgcg ccataaccct tactccttct gggaaatcaa ccagaccaag accatatgga
     3421 actacaaatt ccggcatact tcttcttacc attgtaaagg aatgtagttt acctgtttta
     3481 ctaagtggag cttctactat gtcatcagaa aaacagtgag gacatgcaac atattttgga
     3541 aatgtatatt ttccgcattt attacattta ccacctataa gatgcacatg tggttttgtt
     3601 ccaaaaagtt cagctaaaaa taattcttta tcttcgctca tttttcatat ttctccttat
     3661 tatcttatat tatttagacg ccaagaattg caactacgca ggttatacca tccattgcag
     3721 gttgtgcgcc acccatagtt tcaattattc caacttttgc gccaggaacc tgtctgtctc
     3781 ctgcttcacc tcttaagtgc catacaagct ctgcggtctg cagcagacct gttgctccta
     3841 tgggatgtcc tcttgaaaga agtccaccgc ttggatttac acaaacacta tccccgccca
     3901 aagatgtacg gccgctatct gtaagttctg caccttctcc tttcttgcaa aaaccaagtg
     3961 cttcaacaac aatcatctcg gcaacagtaa atgcatcatg tatttcagca agatccatgt
     4021 cttctgggcc caggcctgcc atttcatatg ccatagatga ggttcttgca acattttctg
     4081 aaggatcaca tattaattta tcgggtgtgt tgtatttacc agattgtgaa atacatccaa
     4141 gaattttaac aggctgctta gttgccagtt cttttacttt gtcttctgaa caaagaacag
     4201 ctgctgctgc cgcgtctcct accggacagc actgatttct ggtaagtggg tcggcgatca
     4261 ttggtgaatt taaaatttct tcaacggata tctgttttct atactgtgca attgggttta
     4321 gtgctgcgtg ttttctgctt ttaacaacta catttgcaaa ttgttcttcg gttgttccat
     4381 attcagccat atatctcttg gctctcattg cataaagtgc aggcattccc attcctagtg
     4441 caacttcaat atcatcatct tcgagaggta gtggaccgcc tgaaaggatt ctgcttaaat
     4501 tttctacacc aagcgcaagg gcaacatcaa actgaccata agcaatagat ctccatgctt
     4561 cccataatga aagtgttccg cttccgcatg ctccctctac atttacaaca ggctggccta
     4621 caagaccaat ctctttaaat gtcctttgtc ctactcccat accctgatac acatggccgc
     4681 aaaatgcaat ttcgatcttt cttgcatgta ttcctgaatc taaaattgct ttccaagcgg
     4741 catcactgcc aagtacatgt gctcctttgt caggaaaacg gccacagcta gtggtaccaa
     4801 ctcctataat atatacatct ctcataaatt ccctccttaa taaactatga cctgtaaaaa
     4861 agctctatag ttttgtctat tatctatcaa caaaaccatt atatatagaa ttttgaaaaa
     4921 ataaatcaaa attatttaat ttttctatat aaaggctatt attttgcttg acatttattt
     4981 aagaatatta taaaaaatta ttaaggtgat ttatatttat taaggagtta ataaaatgag
     5041 tgatttgtta atagacagcg ttcccaattc cagtgaagtc aaagttagag atgcattgga
     5101 tgcaatggga ataagaagat ccatcagatg gtatgaacct aacaaacctg ttgaaagatg
     5161 gaaagtacaa gccatgcttg aagctgcaag aatagctcct tctgcaggaa actttaatgg
     5221 acagagagct attgttgtat acagagatga agacccagat atttgggagt tcatttcaga
     5281 ctggagtcag attacaaccc agatggctcc aatactattt ttctggtgct acgatatgtc
     5341 aggttatgac gtacagggac aaagtattca cgacctcatg agaacaggtg ctcttgataa
     5401 ggcacatggt tgggaatatg aaagggttaa taaacttttc cctctccctg ctatgcttcc
     5461 agattttatt atgcacagac ttgctgcaat tgaccttggt aatgcaattc agaattctat
     5521 gatagctgct tcagctctag gacttgctac atgcctaaat ggtgcaagcg gtggtgcaag
     5581 aaggaatgta aaaactaaat tcaacctgcc aaatacatat gtattttctt ggcttatgac
     5641 agttggttat cctgctgaaa aaatggatgg cggcggtaca agaccaagac caccatttga
     5701 aagaatgttt tttgaaaaac aggttggtaa ttcatttaaa agagatgaaa aaacagtaaa
     5761 actcctagaa gagcttaact taatccaggc tccaggtcct ctaccaggaa gactcgaaga
     5821 gatcaacaga ctcacaaaga gatttggtct tggcgaagaa cacatgaccg attggaaact
     5881 tgctccatct cagactgata ccgatactgt aacatgtgct gatacaaaac cagtaccttt
     5941 aaccgatgaa gaatttcaag ctactgtacc aggtgatgta cctgtagaaa tgcagcttac
     6001 acctacagtt agaagagagg ttcttgaaaa ttacaggaaa gaaaaaggta tcggagacgc
     6061 agattaatat aaattaggag gcaaataaga tggctgaagt aacaattcag attccagatc
     6121 caatatttga aaacataaaa agaagagccg agcaggttgg taatacccca gagggtatta
     6181 ttgctgtaaa tactattct
//
LOCUS       DBNGSV010000119         5858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1545884, whole genome shotgun sequence.
ACCESSION   DBNGSV010000119 DBNGSV010000000
VERSION     DBNGSV010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1545884"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1170
                     /locus_tag="AC159U_04185"
     CDS             103..1170
                     /locus_tag="AC159U_04185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336288.1"
                     /translation="MMDEKKAETFADNLVNTLNNGALCLMLSIGHRTGLFDTMSKIAR
                     STSEEIAEKTGLNERYVREWLGAMVTSKVIDYDPETEKYNLPPEHSAFLTRAAGADNI
                     AVFSQYISELGSVENEIVECFQKGGGVPYSKFPRFHEIMAEDSGQSVLSTLESHIIPL
                     VPNLSEKLENGISVLDIGCGRGLILTRLAELYPGSKFTGKDLSTEAVEFARQNVKQKG
                     LNNIEFVIKDLTNFDQTAQSESYDFITSFDAIHDQAKPLNVLKGIYRTLKQDGVYLMQ
                     DISGTSHVDKDIEHPIGTLLYTISCMHCMTVSLQQGGEGLGAMWGEEKTRDYLQQAGF
                     GSIETNKLDHDIQNNWYVVKK"
     gene            1296..2372
                     /locus_tag="AC159U_04190"
     CDS             1296..2372
                     /locus_tag="AC159U_04190"
                     /inference="COORDINATES: protein motif:HMM:NF041766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HIR3336289.1"
                     /translation="MKILQYITVLSLVFIISSSARALTTLDVYHTNLKIMGIEIICPD
                     ADLDGFSDSACGGSDCNDSDDTINPDAIEICNGVDDNCNFFTDEGFDEDSDGFTTCNG
                     DCNDEVVGINPGIEEWCFNGIDDDCDGMVDCDDEDDDNDLVDDVIEDGAPGGDGNNDG
                     IPDSWQAHVISIPANILGPDPYVTLETDPQHKLFNAKSVNNPSPSNDPAGVSFPLDFF
                     EFEIELLAHGQESFIILYLPDGVEVNTFYKYGPTPDNPSPHWYNFHFDGETGAQFLED
                     RIVLRFVDGKRGDDDITVNGEIIDPGGPGFIEILEESVESSDGCSLAGSGNKITSNNL
                     INLIILLIPMMVALTSLVRRRNNI"
     gene            2508..5195
                     /gene="acnA"
                     /locus_tag="AC159U_04195"
     CDS             2508..5195
                     /gene="acnA"
                     /locus_tag="AC159U_04195"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3336290.1"
                     /translation="MSNFNPKDAKLELETKDGKKVYYSITKLQEISGKDISRLPFSIK
                     ILLENVLRNYDGRIVNTNDIEAIADWGKSSDPVEVPYKPSRVILQDFTGVPCVVDLAA
                     MRGVVNKLGLDPAKINPIVPVDLVIDHSVQVDHYATPEAFQQNVEREYERNGERYAFL
                     RWAQQAFENFRVVPPGTGIVHQVNLEYLASVVASKDIDGDTVVFPDTLVGTDSHTTMI
                     NGLAVMGWGVGGIEAEACMLGQPLYMLMPDVIGFKLYGQLNEGVTATDMVLTITEMLR
                     KKGVVGKFVEYFGPGLTNMTLADRATISNMAPEYGATMGFFPVDDETLRYLRTTGRSE
                     ELIDTVEKYTKAQSLFRTDDMPDPVYTDSLELDLSTVVSSLAGPSRPQDRISLNDMKS
                     AYHDTMKTRLGDNGGLDKTVDVQLNGTGGELSNGSVVISAITSCTNTSNPSVMIGAGL
                     VAKKAVEKGLSVSPTVKTSLAPGSKVVTDYLEDAGLNTYLDKLGFNLVGYGCTTCIGN
                     SGPLPEAIETAIKENDFNCASVLSGNRNFEGRVHPFVKLSYLASPPLVVAYALAGTVD
                     IDLSKDPIGTNDSGEEVYLKDIWPTQQEVRELIEKSVTPEIFNKEYAKVFEGDDKWKS
                     MASPEGKLYEWNEDSTYVQEPPFFEDFGPDTTDPTDIKGAYVYALLGDSITTDHISPA
                     GSIPKDGPAGKFLISKGVDPYDFNSFGSRRGNHDVMMRGTFANIRIKNRLLPGTEGGV
                     TKYIPGDEDTTMFEASEKYRAEGTPLIVIGGKEYGTGSSRDWAAKGTMLLGVKAVITE
                     SFERIHRSNLVGMGVLPLQFKQGENAQSLGITGFELFDIEGISDLSPRKKLSVTATDK
                     EGNKKQFNVICRLDSPVEIDYIKNGGILQTVLRQMISAN"
     gene            5276..>5858
                     /locus_tag="AC159U_04200"
     CDS             5276..>5858
                     /locus_tag="AC159U_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3336291.1"
                     /translation="MSNEGQTIGINKKTGDIEVPDSPIIPFIEGDGTGPDIWHASQIV
                     FDAAVQKAYKGKKKIIWHEVLAGEKAFDQTGEWLPEHTLEDLKKYVVSIKGPLTTPVG
                     GGIRSLNVALRQLLDLYACVRPVRFIKGTPNPVKYPERIDAVIFRENTEDVYSGIEWE
                     SGTPEAEQVRDFLVNTMKVEIRELSGIGIKPISP"
BASE COUNT         1977 a          941 c         1273 g         1667 t
ORIGIN      
        1 ataataaatc caaaaactat gagcaaacat gtacttcaac tcaatgacaa aatatcagca
       61 aattctaact ctaaaggtgg acctgaattc tggggcttga caatgatgga tgaaaaaaaa
      121 gcagaaactt ttgccgataa tttagttaat acacttaaca acggggcact ttgtctgatg
      181 ttgtccatag gacaccgtac tggattgttt gatacaatga gcaagattgc tcgaagtaca
      241 tcagaagaaa ttgcagaaaa gacaggatta aatgaacgtt atgtaagaga atggcttggt
      301 gcaatggtta catcgaaggt catagactac gacccagaaa ctgagaagta caatttgcca
      361 cctgaacatt cggcttttct aacccgtgca gctggagcag ataatattgc agtattcagc
      421 cagtatattt cagagctggg aagtgttgaa aatgaaatag tcgagtgttt tcaaaagggt
      481 ggtggagtgc cttattccaa atttcccaga tttcatgaga ttatggcaga agacagtggt
      541 cagtctgtgc tatctacatt agagtcacat ataattccat tagtcccaaa tttatcagaa
      601 aaactggaaa atggaattag tgtgctggat ataggatgtg gaagaggtct tatattgact
      661 agacttgccg agctttatcc cggtagcaaa tttacaggta aagacctttc aactgaagca
      721 gtagaatttg caaggcaaaa tgtgaagcaa aagggtttaa ataatattga atttgtaata
      781 aaagatttaa ctaattttga tcagactgca caatctgaat catatgactt cataacaagc
      841 tttgacgcaa ttcacgatca ggccaaaccg ttaaatgttc taaagggtat ttatagaact
      901 ctcaaacaag atggtgtata cctgatgcag gatataagcg gtaccagtca tgtagataaa
      961 gatattgaac atccgatagg tacactgcta tatacaatat catgcatgca ctgtatgaca
     1021 gtttctctgc agcagggtgg agaaggccta ggcgcaatgt ggggagagga aaaaacaagg
     1081 gattatttac aacaggcagg atttggttct attgaaacta ataaattaga tcatgatata
     1141 cagaataact ggtacgttgt aaaaaaataa tataagtaca tacattgtat atattccaat
     1201 aatctcacaa taatccctaa atctaagtat ttacttattg accctcatga aatataatag
     1261 acaagatttt acttaaagtg gggtgttatc taattatgaa gatattacag tatataacag
     1321 ttctctcatt agtctttatt atctcaagct ctgccagagc tctaactaca ttggatgttt
     1381 atcatacaaa tttaaaaata atgggtatag aaattatatg tcctgatgct gatttagatg
     1441 gtttttcaga ttctgcatgt ggaggatctg attgtaatga ttcagatgac actataaacc
     1501 ctgatgctat tgaaatatgt aatggagtgg atgataactg taatttcttt actgatgaag
     1561 gatttgatga ggattcagat ggatttacta catgtaacgg cgactgtaat gatgaggtag
     1621 ttggaattaa tccaggaatt gaagagtggt gttttaatgg tattgatgat gactgtgacg
     1681 gaatggttga ttgtgatgat gaagatgatg ataatgacct tgtggatgat gtgattgaag
     1741 atggcgcccc aggtggagat ggcaacaatg atggcatacc tgacagctgg caggcacatg
     1801 taatatcaat accagcaaac atattaggac cagatccata cgttacattg gaaactgatc
     1861 cacaacataa attatttaat gctaaaagtg taaataatcc ttctccttca aatgatccgg
     1921 caggtgtaag ttttccactt gatttttttg aatttgagat tgaactgttg gcacatggtc
     1981 aggaaagctt cattatttta tatctaccag atggagttga agtgaatact ttttataaat
     2041 atggtccaac tccggataac ccatcacctc actggtacaa ttttcatttt gacggtgaga
     2101 caggtgcaca atttttagag gacagaattg tactcagatt tgttgacggt aaacgtggtg
     2161 atgacgatat aacagtaaat ggtgaaatca tcgatcccgg cggaccaggt tttattgaaa
     2221 ttcttgaaga gagtgttgag tcttctgatg gatgttctct tgctggttct ggtaataaaa
     2281 ttacatctaa taatttaatt aatctaataa tacttctaat tccaatgatg gtagcattaa
     2341 cttcattagt cagaagaaga aataatattt aataataaat attaacaaca tatatttcta
     2401 tatcttttaa taatagaata attttctatt cttagaattt atctatttaa attcccgtat
     2461 tatttggtaa tatatcaagc ccgaaaataa taaaagaagg tggtattatg tctaatttta
     2521 atcccaaaga tgcaaagctt gagttggaaa caaaagatgg gaagaaggtt tattattcca
     2581 ttactaaact acaggaaata tccggaaagg atatatccag acttcctttt tcgataaaaa
     2641 ttctattaga aaatgttctt agaaattatg atggtcgtat agtaaataca aatgatattg
     2701 aggcaatagc agactggggt aaaagctcag acccggttga agtaccttat aaaccatcaa
     2761 gagtaatcct tcaggatttt accggtgtac cgtgcgttgt agaccttgcg gctatgcgtg
     2821 gggttgtgaa taaacttggg cttgatccag caaagattaa ccctattgtt cctgttgatc
     2881 ttgttatcga tcactccgtg caggttgatc actatgcaac ccctgaagca ttccagcaga
     2941 acgtagaaag agaatacgaa agaaatggcg aaagatatgc atttttaaga tgggctcaac
     3001 aggcatttga aaatttcaga gttgtaccac cgggaacagg aatcgtacat caggtaaacc
     3061 tcgaatacct tgcaagtgta gtagcctcaa aggatataga cggagatacg gtcgtttttc
     3121 ctgatacact cgttggaaca gactcacaca caactatgat taatggctta gccgtaatgg
     3181 gctggggagt aggcggaatt gaagctgagg cctgtatgct ggggcagcct ctatatatgc
     3241 ttatgccaga tgttataggc tttaaactat atggacagct aaatgagggt gtaacggcta
     3301 ctgatatggt acttacaatt acagaaatgc ttaggaaaaa gggtgtagtt ggaaagttcg
     3361 tggaatattt cggtcctggg cttacaaata tgactcttgc agatagggct actatttcaa
     3421 atatggctcc tgaatatggc gccacaatgg gatttttccc ggttgatgat gaaacactca
     3481 ggtatttaag aacaacaggc agaagtgaag aacttattga tacagtagaa aaatatacta
     3541 aagctcagag cctttttaga acagatgaca tgcctgatcc tgtttataca gattcactgg
     3601 agcttgatct gtcaactgtt gtatcgtcac ttgcaggccc aagcagacca caggacagaa
     3661 tttcattaaa cgacatgaag tctgcatatc atgacacaat gaaaaccaga ctcggagata
     3721 atggtggttt ggacaaaact gttgatgtac agttaaacgg aacgggcgga gagctctcaa
     3781 atggctcagt agttatttct gccataacta gctgtacaaa tacatcaaat ccttcagtaa
     3841 tgataggtgc cggccttgtt gccaaaaaag cagtagaaaa gggtctttct gtaagtccta
     3901 cggttaaaac aagccttgcc cctggttcta aggtagtaac tgactactta gaggatgcgg
     3961 gtttaaatac atatttggat aaattgggat ttaatctggt tggatatggc tgtactacat
     4021 gtataggaaa cagtgggcca cttccagagg caatagaaac tgctattaaa gaaaacgatt
     4081 ttaactgtgc atctgttctc agtggaaata gaaactttga gggcagggtt catccttttg
     4141 taaaactatc atatctcgct tcccctccac ttgtagtagc atatgcactt gcaggtactg
     4201 tggatattga tctttcaaaa gatccaatcg gcacaaatga ttctggcgaa gaggtttact
     4261 taaaagacat ctggccaaca cagcaggaag ttagagaact aattgaaaaa tccgtaacac
     4321 ctgaaatatt caacaaggaa tatgcaaagg tatttgaggg cgatgataaa tggaagagta
     4381 tggcaagccc tgagggcaaa ttgtatgaat ggaatgaaga ttcaacatat gtacaggaac
     4441 caccgttttt tgaagatttc ggacccgata ctacagatcc aactgatata aaaggagctt
     4501 atgtatatgc actacttgga gattcaataa caaccgatca catttctcct gccggatcta
     4561 tacccaaaga cggccctgcc ggtaaatttc ttataagtaa aggggttgat ccttatgact
     4621 tcaacagttt tggatccagg cgcggtaacc atgatgttat gatgagagga acttttgcta
     4681 acatcagaat taaaaacagg cttcttccag gaacagaggg tggtgttacg aagtatatcc
     4741 cgggtgatga agacactacg atgttcgaag catcagaaaa atatagagcc gaaggcactc
     4801 ctctaattgt aataggcggc aaggagtatg ggaccggaag ttcaagggac tgggctgcaa
     4861 agggtaccat gcttttagga gtaaaagcag ttataacgga aagttttgag agaattcaca
     4921 gaagtaacct tgttggcatg ggggtgcttc cacttcaatt taaacaagga gaaaatgctc
     4981 aaagtctggg tatcactggt tttgaattat tcgatatcga ggggatttca gacctctcac
     5041 caagaaaaaa gctcagtgtg acagctacag acaaagaagg aaataaaaaa caatttaatg
     5101 taatttgcag gcttgatagt cctgttgaga tagattacat caagaacggc ggtattttac
     5161 aaacagtgtt aagacaaatg ataagcgcaa attaattgca atttataaat ttttatatat
     5221 attcactgtc caaaaaatga gcaaataaga gaatgatgcg aaaggagtat tattgatgtc
     5281 taatgaagga caaacaattg gtataaacaa aaagactgga gatattgaag taccagatag
     5341 tccaataatt ccatttatag aaggagatgg aaccgggccg gatatatggc atgcatcaca
     5401 gatagtattt gatgctgctg ttcaaaaagc gtataaaggt aagaaaaaaa ttatctggca
     5461 tgaagttctg gctggagaaa aggcatttga ccaaacagga gaatggttac cggaacatac
     5521 tctagaagat cttaaaaagt atgtagtttc aataaaaggg ccattaacta caccagtagg
     5581 tggtggaatc agaagtctta atgtagctct aaggcagctt cttgatctat atgcctgcgt
     5641 acgtcccgta agatttataa agggcacacc aaatcctgtt aaatacccag aaaggatcga
     5701 tgctgtaata ttcagggaaa atacagagga tgtgtattca ggaattgaat gggagagtgg
     5761 tacaccagag gcagagcagg taagagattt tcttgtgaat actatgaagg tagaaataag
     5821 agaactttct ggtattggta taaaacctat tagtccgt
//
LOCUS       DBNGSV010000120         1054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_438349, whole genome shotgun sequence.
ACCESSION   DBNGSV010000120 DBNGSV010000000
VERSION     DBNGSV010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_438349"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="AC159U_04205"
     CDS             <1..588
                     /locus_tag="AC159U_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3336292.1"
                     /translation="FLPSRWEGISLSIYEAMSCGLPVVGSDVGGQKELVTPDCGYLID
                     ASNQNEQVQQYSKILTQLIRDKSKRTELGNNARKRICESFTMNMMGETILECMRIAAT
                     KNDSNSNFGISYDDAKTKLLKGMNFLRITRGLHDINSNFTDIKTVNPQFINLRYTLYR
                     SINYFYEPLYMYAKKRGWNWLFTVADKIKRLVLGT"
     gene            615..>1054
                     /locus_tag="AC159U_04210"
     CDS             615..>1054
                     /locus_tag="AC159U_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336293.1"
                     /translation="MKHLIISREYPPAPGGGIGTYVHNISRLLAEAGETVHVISQKWE
                     GAEADTEELFDGKLIIHRIAYMYWGSETGQITPHPDIISDPDYKLFNSDFHHQYFSMQ
                     VAKLAEKLVYEEGINIIESQEYEAPLYYFLLRRACGLGPKSNPP"
BASE COUNT          364 a          172 c          204 g          314 t
ORIGIN      
        1 tttttaccct caagatggga ggggatttca ctttcgatat atgaggcaat gtcttgcggc
       61 ttaccggtag tgggttcgga tgttggcgga caaaaggagc ttgtaactcc ggattgcgga
      121 tacttgatag atgcttcaaa tcaaaacgag caagtacagc aatattctaa aatattaacc
      181 cagctaataa gagacaaatc aaaacgtact gaactaggaa ataacgcaag aaaaagaatc
      241 tgtgagtcat ttactatgaa tatgatgggt gaaacaatat tagaatgtat gaggatagcg
      301 gcaactaaga atgattctaa ttcaaatttc ggtatttcct atgatgatgc taaaactaaa
      361 ctgctaaaag gcatgaattt tctaagaata acaagagggc tgcatgatat taattctaat
      421 tttacggata taaaaactgt aaatcctcaa tttattaatc ttagatatac attatacaga
      481 tcaatcaact atttttatga gccactttac atgtatgcta aaaaaagagg ttggaactgg
      541 ctatttacag tggcagataa aattaaaaga ctggtgcttg gcacataagg tataaaacta
      601 atttatatat ttaaatgaaa catcttatta tatccagaga atatcctcct gcccccggcg
      661 gtggaattgg tacatatgta cataacatct caagacttct tgctgaagcc ggagaaacag
      721 ttcatgtcat aagccaaaaa tgggagggtg cagaggcgga tactgaagag ctttttgatg
      781 gaaagttgat tattcataga attgcttaca tgtattgggg ctctgaaacc ggacaaataa
      841 caccccaccc ggatattatc tctgatccag attataagct tttcaattct gattttcacc
      901 atcaatattt ttctatgcaa gtagcaaaat tggctgaaaa gcttgtatat gaagaaggta
      961 ttaatattat tgaatcccaa gaatatgagg ctccacttta ctattttttg ttaagacgtg
     1021 cttgtggatt aggacctaaa tccaaccctc catg
//
LOCUS       DBNGSV010000121         4376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_24257, whole genome shotgun sequence.
ACCESSION   DBNGSV010000121 DBNGSV010000000
VERSION     DBNGSV010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_24257"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..2020
                     /gene="dnaK"
                     /locus_tag="AC159U_04215"
     CDS             155..2020
                     /gene="dnaK"
                     /locus_tag="AC159U_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3336294.1"
                     /translation="MAIIGIDLGTSNSAAAVLRGGRPVIIPGSEGISLGGKAFPSYVA
                     LTAEGDIIVGEPARRQAAANPEGTATGFKRKMGKREKIQLRDKEFSPEQLSAFLLQKI
                     KRDAEAFLGESVEKAVVTVPAYFDDNQRSATKDACGIAGLEVARLVNEPTAASLAYGL
                     DRLNQDLKIVVIDLGGGTLDVTIMDFGKGVFEVKSTSGDTQLGGTDMDTAIFEYFSDK
                     FKNDTGFDITTDKKAAARLREAAEIAKIELTSTTTTHVSLPFLAAVGGEPCHLDLDFN
                     RTELERLIRPVIDKCIGPVEQALHDASIAPKDIDRIIFVGGPTRMPSIRTYFEDFFGR
                     KAEMGVDPMECVASGAAIQAGVLTGEVKDIVLVDVTPLTLGVETLGGVATALIARNTP
                     IPVKHSEIFTTAADMQTSVTIHVFQGERPMTMDNTSLGQFNLDGIPPAPRGIPKIEVT
                     FDIDSNGILNVEAKDMATGKLQSIRITDSTRLSKNEKERMVSEAEKYADEDKKKREVA
                     EKLNEADSVCYQAEKMLAEFASKLTEELRNKIESGRHDTREAILKKDTDLAVQRAEDL
                     KKILQEAGSTLYAQSTESTPHPKPDVGTQGGEARPSGSGPGGRVVDAEYTDETKD"
     gene            2051..3142
                     /gene="dnaJ"
                     /locus_tag="AC159U_04220"
     CDS             2051..3142
                     /gene="dnaJ"
                     /locus_tag="AC159U_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3336295.1"
                     /translation="MASAKRDYYEVLGIKNDADQKTIKDSFRKLALKYHPDRNKAPDA
                     EERFKEIAEAYAVLSDPKKRTRYDSGGFEGVSGFSHEDLFGGINFEDIFGSTGFDFDF
                     GLGRGGIFDGLFGKRRTGPPKGQNIELNIELPLEQIEKGGEEQIRYNRPQQCSKCKGT
                     GAKPGTEPTKCSDCNGTGRHMQSRQEKGVTMQQITTCTTCMGKGAFIKEYCPKCQGKG
                     LEDKTEIISVKIPPGAEEGMALRIAGHGYPSSVNGGLAGDLYVVVHSSLDPRFQRRGP
                     HLWRTETISVADAALGTKLDVPTLKDKVKVTVPPGTQPGSILRLHDKGLPEFSGDGRG
                     DMFLNIEVHIPEKLNREERKLFERLKEIS"
     gene            complement(3172..3498)
                     /locus_tag="AC159U_04225"
     CDS             complement(3172..3498)
                     /locus_tag="AC159U_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336296.1"
                     /translation="MLKRWKVTPSQMDNMAITYGSCAPDETKYNPVYLYAVIRYSINQ
                     KQCSLWFLQKVGEIWKHDLTMMQKAIMFDNKNRWNFVPGVSHRYGFAFTDWKFDSRGY
                     PISSIN"
     gene            3764..3985
                     /locus_tag="AC159U_04230"
     CDS             3764..3985
                     /locus_tag="AC159U_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3336297.1"
                     /translation="MPVFEYECKCCNSRFETLVMSTDEEVNCTDCGSEDIEKQFSSFA
                     SQSDSASFGSDPADMPTGGGCCGGGCACG"
     gene            4115..>4376
                     /locus_tag="AC159U_04235"
     CDS             4115..>4376
                     /locus_tag="AC159U_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336298.1"
                     /translation="MPNTKIIAVIFGGKSVEHEISIISAKSILKNLDKRKYIPVPIFI
                     DKKGVWRNAAFSNSELKANNKSYLTPLITANKPALFEIGPKQK"
BASE COUNT         1416 a          844 c          945 g         1171 t
ORIGIN      
        1 tccaatacaa acatttgtat tagcttgctt atcttactat ttgatagttt tgtattctta
       61 agtaacttct tttgcatagt ttcagcttag tatactttgc tgtcctatgc gagctcctaa
      121 atttattact taaatatcct aatctggagg cattatggct atcattggta ttgatcttgg
      181 aacttccaat tcagcagcag cggtacttag aggcggaaga cccgttataa tacccggctc
      241 agaaggtata agcttgggag gtaaagcgtt tcccagctat gttgcactga ctgccgaggg
      301 agatattatt gtaggtgaac ctgcaagaag gcaggcagcc gcaaaccctg aaggtactgc
      361 aaccggcttt aagaggaaaa tgggcaagcg cgaaaaaata caattgagag ataaagaatt
      421 ttctcccgag cagctttctg cattcttact tcaaaaaata aaacgcgacg ccgaagcatt
      481 tctaggagaa tcggttgaaa aagcagtagt tacggttccc gcttattttg atgacaatca
      541 aagaagtgct actaaagatg catgtggtat tgccggactt gaagtggcac gtcttgtaaa
      601 cgaacccact gcagcatctc tagcatatgg gctggataga ttgaatcagg atttgaagat
      661 tgtcgtgatt gacctgggcg gcggaactct tgatgtcacc ataatggatt ttggtaaagg
      721 ggtatttgag gttaaatcca caagcggcga tactcaactg ggtggtacag atatggatac
      781 agctattttt gaatatttct cagacaaatt taaaaacgat actggatttg atattactac
      841 tgacaaaaaa gctgcagcta gactgcgtga agcagcggag atagcaaaga ttgagctaac
      901 atctacaacg acaactcatg tcagcctgcc ttttttggcc gctgtaggtg gagaaccatg
      961 ccacctggat ctagacttca accgtacaga acttgaacga ttaattcggc cggttattga
     1021 taaatgcata ggacctgttg aacaggctct gcatgacgca agtattgccc ctaaagatat
     1081 cgaccgtatt atatttgttg gtggtcctac ccgtatgccg tcaataagga cttacttcga
     1141 ggattttttc ggcagaaagg ctgaaatggg agtcgatcca atggaatgtg tagcaagcgg
     1201 tgctgcaatt caggcaggag tactcacagg agaagttaaa gatattgtac ttgtagatgt
     1261 tactcctctc acactaggtg tagaaacctt aggcggtgtt gcaactgcat taattgcaag
     1321 aaatactcct ataccggtta aacattctga gatttttaca actgcggcag atatgcagac
     1381 gtctgtcacg atacacgtat ttcagggtga aagacccatg accatggaca atacatctct
     1441 gggtcagttc aatctcgatg gcatacctcc ggcaccacgt ggaattccaa aaatagaggt
     1501 cacttttgat attgattcaa atggaattct aaatgtagaa gcaaaggaca tggctaccgg
     1561 caaattgcag tcaatacgaa ttacagattc cacaagactt tccaagaatg aaaaagagcg
     1621 tatggtaagt gaggcagaga aatatgccga tgaagataag aaaaaacgcg aagtagctga
     1681 aaaattgaac gaagcagact cagtctgcta ccaggcagaa aaaatgcttg cagaatttgc
     1741 aagcaaatta accgaagaat tacgcaacaa aattgaatcg ggaagacacg atacacgaga
     1801 agcaatttta aagaaagaca cagacctggc agtacagcgt gctgaagatc tgaaaaagat
     1861 tctccaggaa gccgggtcga ctctttacgc ccaatcaacc gagagtactc cgcatcctaa
     1921 accggatgta ggaactcaag gtggagaagc ccgcccttca ggctctggcc ctggaggacg
     1981 cgtagtagat gccgaatata cagatgaaac taaagattaa agcctgctat actcaaaagg
     2041 aatcttctcc atggcttcag ctaaacgcga ctattatgaa gtcttaggta ttaaaaatga
     2101 tgctgatcag aaaaccatta aagactcatt tcgcaaactt gctctgaaat accaccccga
     2161 ccgaaataaa gcacccgacg cagaagagcg ttttaaagaa atagcagagg catacgcagt
     2221 tctctcagac cccaagaaaa gaaccagata cgactccggt ggctttgaag gagtttccgg
     2281 tttttctcat gaggatttat tcggaggcat aaactttgag gacatttttg gcagtacggg
     2341 ttttgatttt gactttggct tgggcagagg gggtatattt gacggattgt ttggcaagcg
     2401 ccgtacaggt cctcccaaag ggcaaaatat agaattgaac atagaacttc ctcttgagca
     2461 aattgaaaaa ggtggagaag aacaaataag gtataacagg ccccaacaat gctctaaatg
     2521 taaaggaaca ggggctaaac ccggaacaga accaaccaag tgcagcgatt gcaatggaac
     2581 gggcagacat atgcaaagca ggcaggaaaa gggtgttaca atgcagcaga taacaacttg
     2641 tacaacctgc atgggtaaag gcgcatttat aaaagagtac tgcccaaaat gtcaggggaa
     2701 agggttagaa gataaaacag agattatttc agtaaaaata cctcccggag ctgaagaagg
     2761 aatggctctt aggattgcag gccatggcta tccaagctcg gttaacggag gtcttgccgg
     2821 tgacttatat gttgtagtac actcatctct tgaccccaga ttccaaagaa gaggtcctca
     2881 tctatggaga accgaaacaa tatctgtagc cgatgcagcg ctgggaacca agctggatgt
     2941 accaacccta aaagacaagg ttaaagtaac ggtaccaccc ggaacccagc ccggatctat
     3001 cctacggctg catgataaag gacttccgga atttagtgga gatggaagag gagatatgtt
     3061 cctcaatatt gaagtgcata ttcctgaaaa actgaatcgc gaagaacgca agctattcga
     3121 acggcttaaa gaaattagct aataaaatta ctattccggt tatagacacc attaattaat
     3181 agaggatatg ggatagcccc tgctatcaaa cttccaatca gtgaaagcaa aaccgtatct
     3241 gtggcttacc cccggtacga aattccaccg atttttatta tcaaacatga tagccttttg
     3301 catcatagtc agatcgtgct tccatatttc tccaactttc tgtaaaaacc atagagagca
     3361 ttgcttttga tttattgaat atcttattac tgcatataaa taaacagggt tatatttagt
     3421 ctcatctggc gcacagctgc cgtaggttat agccatatta tccatctgcg aaggagttac
     3481 tttccatctt ttcaacatct gtttggactg ttatgtataa atagcaagat caggattatt
     3541 attgttttct ttaattaagg ccagatatag cattaacaca tcctcgggtg tctgaggata
     3601 atcaaaagat tttcccagtg cggtaaaatt cttaagcagg aaaaaagttg ccactagaaa
     3661 gaaaacaata tatttatgct cagatttcat gttattttga cttaataatt gtaatcaata
     3721 tacttttgta atatcaaata tttatttagg agataaatat taaatgcccg tttttgaata
     3781 tgaatgtaaa tgctgtaaca gcagatttga aacgttggtt atgagtacag acgaagaagt
     3841 aaactgtaca gattgtggta gcgaagatat agaaaaacag ttttcgtcct ttgcttcaca
     3901 aagtgactcg gcatcttttg gttctgaccc agcagatatg cctacaggcg gcggatgctg
     3961 cggtggtggt tgtgcttgtg ggtaagagtc ttgttctttt ctcatcatct gcttaacata
     4021 cttagcttaa ttaatttcag gtcaatacat atataatttt tatttctcca atttagatgg
     4081 tatctatttt aaaatttatt ggagactatt agccttgcct aacaccaaga taattgccgt
     4141 tatattcggc ggaaaatcag ttgagcatga aatatctatc atttcagcaa agtccatatt
     4201 aaaaaacctc gataaaagaa aatatatacc cgtccctatc tttattgaca agaaaggagt
     4261 ctggagaaat gccgcattta gtaattcaga actaaaagca aataataaat cctatttaac
     4321 acctcttatt acagccaaca agcctgcctt atttgaaata ggtcccaaac aaaaga
//
LOCUS       DBNGSV010000122         1425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1339565, whole genome shotgun sequence.
ACCESSION   DBNGSV010000122 DBNGSV010000000
VERSION     DBNGSV010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1339565"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="AC159U_04240"
     CDS             complement(<1..732)
                     /locus_tag="AC159U_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3336299.1"
                     /translation="MNLQTLRYYSNNLTIIFLLLAIVVASLVSVANYYYFKSVIYDKQ
                     VSQITGAFTEQREFSAAAKELLNTNKDVIYAKLVDGDGVLQQSFGNSENLNTKEIIVN
                     VSDGNKIFVGVNKSVLGFPFEIPVAATTIISLLVLGLFLMALKLYYPFQKNSLSKLNE
                     HLIKVSKGDYSAKLEIDSDLKDDVDMIKVFDSFNELVDSLSGSMVYKEPKDSSVINSV
                     IVNGNGKTHTNGKATTEPVNLQTNET"
     gene            complement(904..1164)
                     /gene="rpmA"
                     /locus_tag="AC159U_04245"
     CDS             complement(904..1164)
                     /gene="rpmA"
                     /locus_tag="AC159U_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013326569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3336300.1"
                     /translation="MSTKKGGGSSKNGRDSAGRRLGIKKFGGEQVIAGNIIVRQRGTK
                     IHPGENVGIGRDHTLFALTDGVVSFQKYSGKRTRINIAPAAN"
     gene            complement(1174..>1425)
                     /gene="rplU"
                     /locus_tag="AC159U_04250"
     CDS             complement(1174..>1425)
                     /gene="rplU"
                     /locus_tag="AC159U_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3336301.1"
                     /translation="VELLNSEDGSNVEFDDVLLVSDDASDVKVGSPTVQGAKVTGKVL
                     GTKKGKKLIVFKFRRRKGFKKKQGHRQKYSSVEITNIQA"
BASE COUNT          415 a          291 c          206 g          513 t
ORIGIN      
        1 tgtttcatta gtttgtaaat ttacaggctc agttgttgct ttaccattgg tatgagtttt
       61 accattgcca tttactatta ccgaatttat tactgaagag tctttaggtt ctttgtagac
      121 catagaacca ctgagtgaat caaccaattc gttgaagctg tcaaatactt ttatcatatc
      181 cacatcatct tttaggtctg agtctatctc gagctttgca gaataatctc cttttgaaac
      241 ttttatcaga tgttcattta atttactaag agaatttttt tgaaatgggt agtataactt
      301 aagagccatc aagaatagtc caagcacaag aagactaatt attgtcgttg ctgctacagg
      361 tatttcaaaa ggaaaaccca gtactgattt atttacacca acaaaaatct tatttccgtc
      421 tgaaacatta actataattt cttttgtgtt aaggttttct gaatttccaa agctttgctg
      481 cagcactcca tcaccatcaa caagctttgc ataaataaca tctttattag tatttaaaag
      541 ttcttttgcc gctgcgctaa attctctttg ctctgtaaac gcaccagtaa tttgggaaac
      601 ctgtttatca tatataacag atttaaaata ataatagtta gcgactgata caagtgatgc
      661 gacgactatg gccaacaata aaaaaattat agtaaggttg ttactataat atcggagagt
      721 ctgtaaattc atttgttcca cctctacaat attattatat tgtatttact gcttccggtt
      781 aattaaacac ctgtgctttt gccagtttac acatggtgca tgttccaatt ccaaatctta
      841 ataaagctgg ttgtttaaga gctaatgtta attataacag atatcaataa taatttgaag
      901 tctttaattt gctgcagggg caatattgat tcttgttctt tttcccgaat atttctgaaa
      961 acttactact ccatcggtaa gagcaaataa tgtgtggtcc cttccaatac caacattttc
     1021 accaggatga attttagttc ccctctgacg aacaataatg ttgccggcta taacctgctc
     1081 accaccaaac ttttttattc caagtcttct tccagcactg tctcttccgt ttttagagct
     1141 tccgccgcct ttttttgttg acatatctgc ctcctaagcc tgaatattcg taatttcaac
     1201 agatgaatat ttctgcctgt gaccttgttt cttcttaaat cccttacgtc ttctaaattt
     1261 aaatactatt aattttttgc ctttcttggt accaagcacc ttacccgtca ccttggcacc
     1321 ctgcaccgta gggcttccaa ccttaacatc tgatgcgtca tcagatacca atagtacatc
     1381 atcaaattcc acatttgatc catcttcaga atttagaagt tctac
//
LOCUS       DBNGSV010000123         8845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_509059, whole genome shotgun sequence.
ACCESSION   DBNGSV010000123 DBNGSV010000000
VERSION     DBNGSV010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_509059"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1484)
                     /gene="mgtE"
                     /locus_tag="AC159U_04255"
     CDS             complement(171..1484)
                     /gene="mgtE"
                     /locus_tag="AC159U_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3336302.1"
                     /translation="MEQTSQLVPFVSKFFDYDPLTATHALETMERAQAVRVLTELPPS
                     ISAKIFKHLQVDFASDLLESVSSDTLKEIMEKLDPQQGALIFLNIEEELRLKLLEHLT
                     DKDKKAIQELLTYPKGSVGNIMSTEYLAFRSDIKVNDAINKIKERSRAKAPPASYVYV
                     IDSENHLIGILNMRDLIVADDYSTLESIMIKNVFTIDAFMQREDAANELSNRKYFAAP
                     VVDAENHLLGIVKADQLINQAQQEASEDIMKMFGVGSEERAFSTLTFSLRKRLPWLHI
                     NLVTAFMAASVVAVFEDIIAKITVLAVFLPIVAGQGGNAGSQSLAVVMRGLVMREISP
                     RESKKMLLKEGKIGFVNGVVIGLVTALVAWLWKGNPYLGLVIGLAMIVNLVAAGLSGA
                     FIPLTLKAIGQDPAQSSSIILTTVTDIVGFFAFLGFAVIFQNLLM"
     gene            complement(1497..2669)
                     /gene="cdaA"
                     /locus_tag="AC159U_04260"
     CDS             complement(1497..2669)
                     /gene="cdaA"
                     /locus_tag="AC159U_04260"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3336303.1"
                     /translation="MADVLGQVGIAGFADMLIMSFIIYSLLVWLKRTQARFILTGIFI
                     IGVIYLLSQQFNLVLTSLILQAFFAVILLAVLIIFQKELRQLFEQVALWSFNPASKSE
                     QVESEFQSSIHIISESLIDFANKKIGALVVLPAKDNIESFLDGGTELNGKLSEELLDS
                     LFDPHSHGHDGAVIIENNKISKFSCHLPLSQNFSQLQKRGTRHAAALGLSENTDALCI
                     VVSEERGEISIARNGKLTKIQNDEIKKYLEDYYTETNPNIIVNSWKDFFRKNYRDKAI
                     AVAASILLWFVFVHESRLIYKSYNIPVTNIVVPSEMKISRIKPEEIKVILSGFKRDFF
                     FYNPNNIKAVIKLPDAVVGDNLVTITRSDFIIPKNLVVERIHPTSMYITLTNQHNQ"
     gene            complement(2773..2970)
                     /locus_tag="AC159U_04265"
     CDS             complement(2773..2970)
                     /locus_tag="AC159U_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336304.1"
                     /translation="MPKIDIDLKLDYKSEDEDTIDDLYLDDILDELGDIIVDHFNVKK
                     DIYGVLNLYKQEKLKKSLNFD"
     gene            complement(2989..3474)
                     /locus_tag="AC159U_04270"
     CDS             complement(2989..3474)
                     /locus_tag="AC159U_04270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR3336305.1"
                     /translation="MSSVDIIIEIPKGSRNKYEYDKEKKAFRLDRMIFSSVHYPADYG
                     YIENTLAEDGDALDALVLLWEPTFTGCIVEGRPVGYLDMKDEKGQDEKILCVPLTDPH
                     WEQIKDIEDVPSHLLKEIEHFFLIYKQLESKNVEVTGWKNKDCALNIINKAQQKFISS
                     E"
     gene            3553..4035
                     /locus_tag="AC159U_04275"
     CDS             3553..4035
                     /locus_tag="AC159U_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR3336306.1"
                     /translation="MIYQLVISLAIAILFLLFRKLLNKIIKDISEKYEYTPARAFLIK
                     KLNTIFWVTVYFSILILIWGISIEGLEFYITSFFAVAGIALFASWSVLSNITSAVILF
                     FEYPIKAGTNIKIQDGDNTIEGEVLNLSLFNIEIKTRSGVVFYPNNLAIQKAIVKKQK
                     "
     gene            complement(4045..5121)
                     /locus_tag="AC159U_04280"
     CDS             complement(4045..5121)
                     /locus_tag="AC159U_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR3336307.1"
                     /translation="MIIFFLILGLIFLIAGAELLVKGSSRLASSFGISPFLVGLTVVA
                     FGTSSPELAVSVKAALSNQASIGLGNIIGSNIFNILFIIGISAIIRQLFVTKQLLKQE
                     VPLIIFVSGLVLIFALDGNFNRLEGLLLLAGFAIYMIFLVRQSRIEQAKPGDDFKTEF
                     TLREDTIVSIPRNTLFIILGLGLLVLGSKWFVNSAVSIAEYFEVSKIVIGLTIVAAGT
                     SLPEVVTSVLATIKGERDIAVGNIIGSNLFNIMAVLGLSSLVAPVGIQIAPSVIGFDI
                     PIMIIISIICFPIFYTDSLMSRLEGGLFLGCYIAYISYIVFISSNNLTLPLLNNMMLY
                     ILVAVCFLIFIGVIIKPFINLFKK"
     gene            complement(5416..5574)
                     /locus_tag="AC159U_04285"
     CDS             complement(5416..5574)
                     /locus_tag="AC159U_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336308.1"
                     /translation="MPATDNNILNIRYDGAVVNFKNTKIASTTDSYVVSPLFTGDRFR
                     TAECKWDG"
     gene            complement(5780..6424)
                     /locus_tag="AC159U_04290"
     CDS             complement(5780..6424)
                     /locus_tag="AC159U_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3336309.1"
                     /translation="MPGSLDDDSNDEKTIFLGDNISTGYHGAVCGGIKPGDTVAVLGD
                     GAVGLFATYAATLFDPAKVITIGHHDYRLEIAKKYGASELINSNNEDLEERIIEITNG
                     TGADVIIECVGTSECLQQSLNSVRPGGTVSFVGLFWEPFPLNVTDFFLRNLTLKGGVA
                     PSRTYIPKLLPLVEADKFDPSLVISHRISLDEVPKGYELMNNRKENAIKIVFRP"
     gene            complement(6470..6835)
                     /locus_tag="AC159U_04295"
     CDS             complement(6470..6835)
                     /locus_tag="AC159U_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3336310.1"
                     /translation="MKAARAYGIEDVRVEEVPNPKIENPTDVIVKVTMGGICGSDLHI
                     YRGHFQLEEGDGVGHEFVGRVEEVGSDVKTLKKGDKVIGPFWVSCGHCTYCEKGLTTS
                     CLNGGCFGFGNLLGGHPGC"
     gene            complement(6973..7698)
                     /locus_tag="AC159U_04300"
     CDS             complement(6973..7698)
                     /locus_tag="AC159U_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjF family protein"
                     /protein_id="HIR3336311.1"
                     /translation="MDKEHANIFLTAEWKYLAMVNYLIDPEILIPYIPNGTELDDYNG
                     NNFVSLIGFRFINTRLKGISIPFHTDFEEINLRFYVKRKSDEGWRRGVVFIKEIVPKT
                     MIAFVANNLYNENYIALETRSDLRFKDDILSEVKYSWKINNNWNYISMSVNGEKKELK
                     AGSEPEFIAQHYWGYSKRKDVTVEYRVEHPKWSYWDSVQTETSVDVAKLYGQEFVDAL
                     SSNPHSAFLAEGSDVTVSHGKNL"
     gene            complement(7714..>8845)
                     /locus_tag="AC159U_04305"
     CDS             complement(7714..>8845)
                     /locus_tag="AC159U_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3336312.1"
                     /translation="GIDIDIPDQLKPYCAVIKIPEPSIKQYEDLLHHVLRDLQSKIDV
                     QFKLDSDDLNKLLNNLKGLTLMEAEKVITKAIVEDGTLAPKDINRVIDEKIKVVEREG
                     LLEYYPNEETMDSVADLRSLKSWLAKRKEIILKPDKAREFGLSFPKGILLLGVPGCGK
                     SLCAKAVAMEWGLPLLKLDPSNLYNKYIGESEKNFKRAMQTAEKLSPVILWIDEIEKA
                     FSSVSGDHDAGVSTRIFGTFLSWLQDRNGDVFIVATANDVQKLPAEFLRKGRFDEIFF
                     VDVPDPESREAIFEIHLEKRGKDAGKFDMKSIVEATTGFSGSEIEQVIVSALYTAFSN
                     DLELSTTLLLDEIRVTKPLTVTMSDRISNLREWAKERTVSAH"
BASE COUNT         2712 a         1816 c         1432 g         2885 t
ORIGIN      
        1 ttcttcgatt tatccgcaat tgcaggtgtt ttaaattctt ccatcataat aaattttaac
       61 agtaaatatg atattactaa atcattctat atgaaatagc agaattatta gataagaaaa
      121 ctatcaataa tacagatcaa taaaaatatg attacgtatt cattaacaca tcacattaag
      181 agattttgaa atatcacggc aaaccccaag aatgcaaaaa acccgactat atctgtaacg
      241 gttgtaagta tgatactgga agattgagca gggtcctgac caatggcttt tagggtaaga
      301 ggaataaacg ccccggacaa gccggcagca accaggttca ctatcattgc aagtcctatt
      361 actaaaccta aataaggatt tcctttccaa agccaggcca caagcgcagt tacaagcccg
      421 ataacaactc cgtttacaaa acctatcttg ccttctttta aaagcatctt cttcgattct
      481 cttggtgata tctccctcat aacaagccct ctcataacaa ctgcaagtga ctgcgagcct
      541 gcatttccgc cctgtccggc tactattgga agaaaaacag caagaacggt gatcttggca
      601 attatgtcct caaacacagc aacaacgctt gccgccataa atgctgttac gagatttata
      661 tgcagccacg gcagtctttt tctgagtgaa aaagttagtg tagaaaatgc tctctcttca
      721 ctgcccacac caaacatctt catgatgtct tcagaggctt cttgctgagc ctggtttatt
      781 agttgatccg ccttcactat tccaagcaga tggttttcag catctaccac aggtgcggca
      841 aaatatttcc tgtttgaaag ctcatttgca gcgtcttctc tttgcataaa tgcatcaatc
      901 gtaaacacgt tttttatcat gatgctttct aatgttgaat aatcatcagc cacaatcagg
      961 tctctcatat ttaagatacc gataagatga ttttcagaat caattacata gacgtaagac
     1021 gctggagggg ctttagccct tgaacgttct tttatcttat ttatagcgtc attaacttta
     1081 atatcgcttc taaaagcaag atattcggta gacattatat tgcccacact gcctttggga
     1141 taagtaagaa gttcctgtat tgccttttta tccttgtctg taagatgctc aaggagtttt
     1201 aatcttaatt cttcttctat atttagaaaa attagcgccc cctgctgtgg gtcgagcttt
     1261 tccattattt ctttgagtgt gtcggatgat accgattcta ataagtcaga agcaaaatca
     1321 acctgcaggt gcttgaatat ctttgcagaa attgaaggtg gaagctctgt caataccctc
     1381 acagcctgtg ctctttccat agtctcaagg gcatgggttg cagttagtgg atcataatca
     1441 aaaaatttgc ttacaaaagg tactaattgg cttgtttgtt ccatactatg atcctcctat
     1501 tgattatgtt ggttcgtaag agtgatatac atactcgtgg gatgaatcct ctcaactaca
     1561 agatttttag gaatgatgaa atcacttcta gtgatagtca ctaaattatc cccaacgact
     1621 gcatcaggca atttaataac ggctttaata ttattgggat tatagaaaaa gaaatccctc
     1681 ttgaaaccgg ataatattac cttaatttct tcaggtttaa ttctgctaat tttcatttca
     1741 gaagggacta ctatattagt tacgggaata ttataacttt tgtatataag gcgagactca
     1801 tggacaaata caaaccatag aagaatagaa gctgctaccg ctattgcctt gtctctgtag
     1861 tttttcctaa agaagtcttt ccatgaatta acaataatat taggatttgt ttccgtataa
     1921 taatcctcta agtatttttt tatttcatcg ttttgtattt ttgtaagctt tccatttctt
     1981 gctattgata tttctcccct ttcttccgac actacaatac aaagagcatc ggtgttttct
     2041 gaaagtccaa gagccgctgc atggcgggtg cctcgttttt gaagctggct gaagttttga
     2101 gataaaggga gatgacagga aaatttagag atcttattat tttctatgat aactgctcca
     2161 tcatggccgt gtgaatgagg gtcgaaaagg ctatccaaaa gctcttcact cagttttccg
     2221 ttaagctcag taccaccatc aagaaaactt tctatattat cttttgcagg aagtacaacc
     2281 agagccccta tttttttatt tgcaaagtct ataagagatt cagatattat atgtatgctg
     2341 gactgaaact cactttcaac ctgttcactt ttcgatgctg gattaaagct ccaaagtgct
     2401 acctgctcga aaagctgtct tagttctttt tgaaatatta ttagaactgc caaaagaatt
     2461 acagcaaaaa acgcctgcag gatcaaagaa gttaaaacta agttaaactg ctgtgataat
     2521 aggtaaataa ctcctataat aaaaataccg gtgagtataa aacgggcctg ggttctttta
     2581 agccagacaa gaagggaata gataataaaa gacatgatca gcatgtcggc aaagccggct
     2641 atgcctacct gccccagtac atctgccaag acttttttaa tttctacaat ataccccttt
     2701 atacttcaat ttttataaat atattgtatg aaaattttac tacaaccacg tttactttat
     2761 cattctgatc aattaatcaa agttcaaaga tttcttgagt ttttcttgtt tatataaatt
     2821 taaaacacca tatatgtctt tttttacatt gaaatgatca actataatat ctcccagctc
     2881 atccaatata tcgtctaaat aaagatcatc tatcgtatcc tcatcctcac ttttgtaatc
     2941 aagctttaaa tcaatgtcta tcttaggcat ctttgctcct ttaagtcgtt attcactcga
     3001 aataaatttt tgctgagctt tattgataat atttagagca cagtctttat ttttccaccc
     3061 tgtgacttct acatttttac tttcaagttg cttgtagatt aaaaagaagt gttcaatttc
     3121 ctttaacaaa tgagagggca catcctcaat atccttaatt tgttcccagt gaggatcggt
     3181 aagtggaacg cacaaaattt tttcatcctg ccctttttca tctttcatgt ccagatatcc
     3241 cacgggtctg ccttccacaa tgcatcctgt gaatgtaggc tcccaaagta aaacaagtgc
     3301 atcaagcgca tcgccgtctt ctgcaagggt attttctata tatccataat cggcggggta
     3361 atgcacagaa gaaaatatca ttctgtccag cctgaatgcc tttttttctt tgtcatattc
     3421 gtacttgttt ctgctcccct tggggatttc aataattata tcaacactgg acaaatgttt
     3481 atcccataca catatttaag ttataatgtc tacaaaatat acattaatta ttgaaagtct
     3541 agaggcataa aaatgattta tcaattagta atttcattag ctattgcaat actttttttg
     3601 ttgtttagaa aactgctgaa taaaatcatt aaagacatct cggaaaagta tgaatataca
     3661 cctgcaagag ctttccttat caagaaacta aacactattt tttgggtcac tgtgtatttt
     3721 tctatcctga tattaatatg gggaataagc atcgagggtt tagagttcta tattacaagt
     3781 ttttttgcag tagcaggaat agccctcttt gcatcatggt cggtactttc taatattaca
     3841 tccgccgtga tactcttttt tgaatatcca atcaaggctg gaaccaatat caaaatacaa
     3901 gacggtgaca ataccataga aggagaagtc ttgaatctct ctttgtttaa catagaaatt
     3961 aaaaccagaa gcggcgtcgt tttctatcca aacaatttag caattcaaaa ggcaattgta
     4021 aagaagcaaa aatagtatga agatttattt tttaaacaag tttataaatg gcttgattat
     4081 tactcctatg aatatcaaaa aacatacagc aaccagaatg taaagcatca tattatttaa
     4141 aagcggtaaa gttaaattat tggaagagat aaaaactata tatgatatat atgcaatgta
     4201 acatccaaga aacaagcctc cctccaacct gctcatcaag ctgtccgtat agaaaatcgg
     4261 aaagcatata atagaaatta ttatcattat aggtatatca aaacctataa ctgatggagc
     4321 tatctgaata cctactggag caaccagact gctcaaacca agaacagcca tgatgttaaa
     4381 tagattactc cctataatat ttcctactgc tatatcacgc tctcctttta ttgtagctaa
     4441 aacagatgta accacttctg gcaaagaagt ccctgcagcc acaatagtaa gaccaattac
     4501 aatcttgctt acttcaaagt actctgcaat tgatactgcg ctgtttacaa accatttcga
     4561 tcctagcacc aataacccaa gccctagtat aataaataga gtgtttcgtg gaatactaac
     4621 aatggtgtct tctctaagcg taaattcagt tttaaaatca tctccaggtt tcgcttgctc
     4681 tatgcggctt tgacgaacca agaagatcat ataaattgca aaacctgcta gcagtagtaa
     4741 gccttctaat ctattaaaat ttccatcgag agcaaatata agaaccaacc cggatacaaa
     4801 gataattaaa ggtacttctt gttttaataa ctgtttagta acgaatagct gtcttattat
     4861 tgccgagata ccaattataa agagaatatt aaaaatattg ctgccaatta tattacccaa
     4921 cccgatacta gcctgattgg agagtgccgc tttgacactc actgcaagct ctggcgagct
     4981 tgttccaaat gccactacag taagaccaac aagaaagggt gatatgccaa atgaagatgc
     5041 cagacgggac gagcctttaa ccaatagttc agccccggct ataagaaata tcaatcctaa
     5101 gataagaaag aatattatca ttattaataa tgataatata taattataag caatctagta
     5161 tagatataaa acaggttatc aaagattatt ttttgatctg caattagaat aaatatcaaa
     5221 caagaagaat agtcctttgc aagaatactc taagaatcta ttgtaaggtt atattaacaa
     5281 gtttaattat tttactttta attaaaatat agcagttgtg acatctttca attattataa
     5341 tatctaatta gcaatagctg caattataag cctatactta cataaagaga ggtagcccga
     5401 cacattgcaa aatctttaac cgtcccattt acattcagct gtcctaaatc tatccccggt
     5461 gaaaagagga gaaacaacat atgaatcagt cgtagaggca atcttagtat ttttaaaatt
     5521 aactactgcg ccgtcgtagc ggatatttag gatattattg tcagtggctg gcatgaacgt
     5581 cttcaccata atattgcaca taagcttgcc tccgctatga acaatttgta tttggggagc
     5641 ttgacaataa atgaggatat agactaagca cttgttaatt gctgttatta cgactacaaa
     5701 ctaatcggtt taatcctcta ttaccttatt ggatcagccc tgtactctat gccctgaagc
     5761 aggatgcaat aaaaactatt tatggcctga atacaatctt aattgcattt tcttttctgt
     5821 tgttcataag ctcataaccc ttgggaactt catcgaggga aattctgtgg cttatcacca
     5881 gtgaaggatc aaacttatca gcttctacaa gtggcaggag ttttggtata taagttctac
     5941 ttggagctac tcctcctttg agggttaagt ttcttaaaaa gaagtcagtc acatttagtg
     6001 gaaacggctc ccagaaaagt cctacgaatg atacagtgcc acccggtctt actgagttta
     6061 gagactgctg cagacattca gaagttccta cacattctat tattacatct gctcccgtgc
     6121 cgtttgtaat ttctattatt ctttcttcca aatcctcatt gttagagttt attaactcag
     6181 atgccccgta cttttttgca atctcaagcc tgtaatcatg atgcccgatc gtaatcacct
     6241 ttgctggatc aaaaagagtt gcagcatatg tagcaaaaag gccaacagct ccatctccca
     6301 gtacagctac tgtatctccg ggttttatgc ctccgcaaac cgctccatga tatcctgtgg
     6361 agatattatc acccaggaaa atagtctttt catcattgct gtcatcatca agactacctg
     6421 gcactttaac cagtgtaccg tcagcatgag caacctttat atattcagcc taacagccgg
     6481 gatgtccacc aagaagattt ccaaatccaa aacaccctcc attcaaacat gatgttgtaa
     6541 gacccttttc acaataagtg caatgaccac aactaaccca aaatggtcct attaccttat
     6601 cacctttttt aagtgttttt acatcactgc ccacttcttc aactcttcca acaaattcat
     6661 gaccaactcc gtctccttct tcgagttgaa aatggcctct atatatatgc aggtctgatc
     6721 cgcatatacc acccatagtt actttaacta tcacgtctgt tggattttca atttttggat
     6781 taggtacttc ttcgactcta acatcttcta ttccataagc cctggctgct ttcataattt
     6841 atctcccgta tattagtttc aataatatat taactggttt ttctttgttg tataattgtt
     6901 caactattta actggtattc atatctggca tgatgcattg tagctgagaa tgtttcagga
     6961 atttactcag gatcagaggt tctttccatg tgacactgta acatctgaac cctcggccag
     7021 gaatgctgaa tgtgggttgg aagaaagtgc atcaacaaac tcctgtccat acagttttgc
     7081 aacatccaca ctggtttctg tttgaaccga atcccaatag ctccattttg gatgttcaac
     7141 acggtattct actgttacat cttttctctt actgtaaccc cagtaatgtt gagctataaa
     7201 ttctggctcg ctacctgctt taagctcttt tttctcacca tttactgaca tggaaatata
     7261 attccaatta ttatttattt tccatgaata ctttacttcc gatagtatat cgtctttaaa
     7321 tctcaaatca cttctggttt caagtgctat atagttttca ttgtataagt tattagctac
     7381 aaatgcgatc atggttttag gtactatttc ttttataaag acaactcctc ttctccagcc
     7441 ttcatctgat ttcctcttta catagaaccg tagattaatc tcttcaaaat cggtatggaa
     7501 agggatggaa attcctttta gccttgtatt aataaaacga aaccctataa ggcttacaaa
     7561 attattaccg ttatagtcat caagctcggt tccatttgga atgtaaggaa tcagtatttc
     7621 ggggtcgatc aggtaattga ccatcgccag atatttccac tctgcggtca aaaatatgtt
     7681 ggcatgctct ttatccatga ttgattttat taactaatga gcgcttacgg ttctttcttt
     7741 tgcccactct cttaagttgc tgatgcggtc actcattgtc acagtaagtg gtttggttac
     7801 ccttatctca tcgagcagta aagtagtgga taattcaaga tcattcgaga atgcagtata
     7861 tagggcagac acaattacct gctcaatttc tgatccgcta aatcctgtag ttgcttcaac
     7921 tatggatttc atatcgaatt tgccggcgtc cttacctctt ttttcaagat gaatttcaaa
     7981 tattgcttct cttgactcgg ggtctggcac atctacaaaa aatatctcat caaatcttcc
     8041 ttttcttaaa aactctgcag gaagcttctg tacatcattt gcagtggcta ctataaagac
     8101 atcaccattt ctgtcctgca gccatgaaag gaatgtaccg aatatccttg tagatactcc
     8161 tgcgtcatga tcccctgata ccgaagagaa tgctttttca atttcatcta tccaaagaat
     8221 cacgggagag agcttttcag cggtctgcat tgcacgttta aagttcttct cactctcgcc
     8281 aatgtattta ttatacaggt ttgaagggtc cagctttaga agcggtagcc cccattccat
     8341 ggccactgct tttgcacaaa gactctttcc acaacctggt acgcccaata acaaaatccc
     8401 cttaggaaac gagagcccaa attctcttgc cttatcaggt tttagaatta tctctttgcg
     8461 ctttgctagc catgatttca agctcctgag gtcagcaaca ctgtccatcg tctcttcatt
     8521 tggataatat tccagcagac cctcacgttc cactacttta attttttcat caataaccct
     8581 gtttatatcc tttggagcaa gagtaccatc ttctacaatg gcttttgtaa taaccttctc
     8641 tgcttccata agagtaagcc cttttaagtt attaagtaat ttgttcaggt catcagaatc
     8701 aagcttaaac tgcacatcta tctttgattg caggtcacgc agcacatgat gaagcagatc
     8761 ttcatactgt ttaatactag gttcaggtat ttttataaca gcacaataag gcttcaactg
     8821 atcaggaata tctatatcaa tccct
//
LOCUS       DBNGSV010000124        13415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1131676, whole genome shotgun sequence.
ACCESSION   DBNGSV010000124 DBNGSV010000000
VERSION     DBNGSV010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1131676"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="AC159U_04310"
     CDS             complement(<1..1478)
                     /locus_tag="AC159U_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3336313.1"
                     /translation="MLDRTVLIYEQNSKIANYLNSIVKELGFPTTVVTDHEIFLDKAA
                     ELTPGLILFNASKENYKKCLDLANEIFTNLKTSFLFITDKLTAENFIKEKLDFKHICI
                     IEPFKKTQLRDGINSLIDENKSESSKQKTKETSNSENHIGFFTSTVNKEEVKSDVLQN
                     ENNDTAPKVEQSPNDHKSLLKSVLNATYSSNNFTEILNAVATSLQSGMNSVDLVSIYK
                     LEDQKAALICSARPGDKTINIPSSLNYSKGLPWAAIISEDRVFIKDVNNSKYITDTEK
                     QFGVKSFLSVPLKQNGITIGSLSLFSTSDTEAFSEEDLEFFDTVSSQIEMTLGYISKN
                     EELKQSEDRFKSLFDKSPVGVLLLDNDFRVYQCNRRIAEILQTTFDEIVGMDIRELGN
                     EKFTAASRKMLKGSFSRCEFNLKIAGKRKDLWLMASLSPSKDSNDEVTGGMIVLEDIS
                     GRKKMENAITEEKEMLSTTLRSIGDGVITTDSDGNILLFNRVA"
     gene            complement(1675..3039)
                     /locus_tag="AC159U_04315"
     CDS             complement(1675..3039)
                     /locus_tag="AC159U_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR3336314.1"
                     /translation="MLQINPGIFREYDIRGIVDKDLSEQIVEIIAKAYGTYLSNHGKK
                     IVSLGRDCRESSPLYSQTFCKGLNSCGIDVVDLGMVSTPMVYFSLFNLEVGGGAMITA
                     SHNPSDFNGIKLCIGRESLYGSQIQEIRQIAESGEFAAGNGKTEKTDIMDLYINYLTE
                     NIDIKPGLKVGIDCGNGMGGLVGPDIIRRKGCEVTELYTKPDGNFPNHHPDPTVEENL
                     QDLIKVVKENCLDIGIGFDGDADRIGVVDENGNIVWGDMLMLILARDVLKEVPGAQII
                     GDVKCSGRLFNAIEQSGGKPLMWKTGHSLIKSKMKEQGAELAGEMSGHIFFSHRFFGY
                     DDAVYAALRVLEILSKTGKTVSDLLSGVPEALSTPEIRVDCPDDIKFKAVDEVKKELS
                     SQYEVNGIDGLRIEFPDGWGLLRASNTQPAVVLRFEAQDENRLKEIRNIVETPLKEAV
                     NRLS"
     gene            3235..4719
                     /gene="trpE"
                     /locus_tag="AC159U_04320"
     CDS             3235..4719
                     /gene="trpE"
                     /locus_tag="AC159U_04320"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR3336315.1"
                     /translation="MIFPTFTEFKKKLDKGNLVPVWSEFLADFDTPVSALKKIDKGKY
                     SFLFESVEGGEKWGRYSFLGTEPTVIFRSKGNKIEIIENGKTKTEQGNPIDRLRVLMS
                     KYKPVPASELPRFSGGAVGFFSYDIVRFVEDLPVDTTDDLGCWDCLYMITDTILVFDN
                     VSHKIKIISNAFAENKKDAKEAYDNAIKKIGELKSRISKKASFYDNQATAKKRSKSKP
                     AIKSNFKPQDYKEAVNKTKKYIREGDIIQAVISQRWKTKLNTDPLDLYRALRILNPSP
                     YLFYLKMGTEYLVGSSPEVMVRVEGSNVENRPIAGTRPRGKNESQDNKYEKELLADPK
                     ERAEHIMLVDLARNDLGRVAKVGTVKVDEFMTVERYSHVMHIVSNVIAKLKRGKDAFD
                     VIKATYPAGTLSGAPKVRAMEIIEEMESTRRGPYGGAVGYFSFSGNMDTSIVIRTFYI
                     KDGSIYFQAGAGIVADSVPQNEYNECVNKAKALVKAIELTESGL"
     gene            4740..5318
                     /locus_tag="AC159U_04325"
     CDS             4740..5318
                     /locus_tag="AC159U_04325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3336316.1"
                     /translation="MILMLDNYDSFTYNLVHYLGELGQDVVVHRNDKITVEQIEQADP
                     DIIMISPGPCTPKEAGISMDLVNKFKGVKPIFGVCLGHQSIAEAFGAKIIKAKKLLHG
                     KTSEIFHDGKGIYKGIENPFVATRYHSLIIDKKTLPKDFEISAWTKEGEIMGIRHKKL
                     QIEGVQFHPESILTERGKDLLKNYLEIQEQLS"
     gene            5360..7087
                     /locus_tag="AC159U_04330"
     CDS             5360..7087
                     /locus_tag="AC159U_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3336317.1"
                     /translation="MKLLFRPRYEQHLGKRELPFGTFDDPATWLPITRYLEQGAEKFP
                     RKTLFKVADNDGVLVEQYSYEQANGWANRVANALSSKYKIQKGDRIGIYMLNCSEFIF
                     SILGIHKVGGVQVSINKDEKGERLSYIINYSEMKALLVDKSSLPFLQEVSDQLTNLEV
                     IIITDDPQNIPSRIANIPCISFSSFSSFSKFNHNLLVKTSDVERCMFTSGTTGMPKGV
                     LREHGGVILTVRGYLQQHGVRSGDTLMSVLTLAHANAQVMCLFASIAAGATAVFYPRF
                     SASSFWKWAYECGATVTNMLGAVAEYLWAAEPTEWDRKHNVRTVLSGPAPKNLEEFQS
                     RFNTRTIDGYGSTEMGMVLWKDPEDHRLGSSGYPMQGYYVELRNPLNHNEVLSPMWDP
                     TEDQTPPEDAQGLLFIRPLIPHTTLNEYFKDEQRTKEAFDEEGFFNSDDLFARGMDGR
                     YYFVGRYSRIRVSGENVDPVAVANEALKYDSIHEAIAVGIRLPNVADDEIKLNLTIKQ
                     GASFDPVEFCKWMADRVIIAMVPRFIEVYESGFPTTATQKVRVAELKELSEKTWDRNE
                     SGLKFSARK"
     gene            7171..9897
                     /gene="polA"
                     /locus_tag="AC159U_04335"
     CDS             7171..9897
                     /gene="polA"
                     /locus_tag="AC159U_04335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3336318.1"
                     /translation="MAQKDTVYLIDGSSYIFRAYHAIRILSNSKGFPTNAIYGFTNML
                     LKFLKDYDPKYIGIVFDSKGDNFRNEIYPDYKANRGEPPDDLKPQFEEIFGLVKAFNM
                     PMVLMEGYEADDIIGTLAKRFESENMDVVIVTGDKDFTQIVDDSITLLDTMKDKVTET
                     KDVVEKYGVEPDKIIEIFALTGDSIDNIPGVRGIGGKTATTLIQQYGSLENVYNNLDK
                     LKPRQKELLENSKENALLSRELVTIKSDIEMDIDLDEYEYTGFNKEKLIELFKQYEFS
                     SLLKELDTDGKEKIQTNQQTNSANKSDNSVTYDNYNLITNEKDFEKVIDLINKTKTLS
                     IDLETTSQLPMEAEIVGFALCPRPHQSYYVPVGHRNLLDAKNQLSLQYVCDRLKPIIE
                     DEQIKKIGQNLKYEYVVLSKYDIELKGIYFDTMIAAHLIDSSRLSYSLDELSKNYLNH
                     KPISYKDVTTEGRFKITFEEVDIELAKTYACEDADVAYLLSKILLEKLKELDVLEVYN
                     KYLLELIVALAGVEISGVRIDTDKLNHLSKEFEKIILDTEEKVYKESGTKFNLNSTQQ
                     LREVLFDKLGLPAKKKTKKGEPSTDVEVLTDLSKDHIVPKMMLEYRALTKLKSTYIDS
                     LPKLINSKTGRIHTSFNQVGTSTGRVSSSDPNLQNIPIRSEEGMKIREAFVAEKDYKI
                     LSADYSQIELRLLAHFSEDKNLLSAFISGSDIHNRTASEIFGVKQEDVTADMRRLAKN
                     INFGIIYGISPFGLAKQIGTAVNQAKDYIDQYFSRYSSVKQYLDDSVRNAQELGYAET
                     LIGRRRYIPELRSSDRSARGFAERAAINTPIQGSAADVINTAMININKEIRNNYKSKM
                     ILQVHDELLFEVHEDEIKAFTEFIKTKMEGAWKLKVPLIVDINIGNSWAEAH"
     gene            9916..11334
                     /gene="dacB"
                     /locus_tag="AC159U_04340"
     CDS             9916..11334
                     /gene="dacB"
                     /locus_tag="AC159U_04340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3336319.1"
                     /translation="MRSKYLIYLTLGIFISLVCYKSSDAGYSEYSTRLHNIIPASAKK
                     NNKLGVVVKSLINKKKIFEYNSDTLFIPASNQKVIVSTVALSLLKSKFRFKTEFYAGG
                     DIKSGVLHGGLYIKGYADPTINTETLRLIAMQFKQKGLKQINGDIYLDDSYFDDLNYP
                     VGWKSEWRDDFYSPPISALSLNYNNIEVVAYPSKNRSKVVVTTDPKVPGLKIINSAYL
                     KKKKNSIVARWADGKEQIIVSGRLYTYSKGVRLKLPVKYPSLYFGRVFKNGLAEQGIT
                     ITGQVKFANVPRWANLYHKYESDRLDEVVNEYNKKSVNIIGENLIKTLGAEYIGTPGS
                     WEGGRKVISLFLKNIGIKDGFKIVDGSGLSLLNRVSPNALADVLEYAFQNKSVGLDFI
                     TSLPVAGIDGTLRKRFKDKDVKGRIVAKTGYLKNVRSLSGYVFTTTGDVLVFSILSNG
                     LGHEAKKFQSDLLAELVSCCEG"
     gene            complement(11331..12164)
                     /gene="rsmA"
                     /locus_tag="AC159U_04345"
     CDS             complement(11331..12164)
                     /gene="rsmA"
                     /locus_tag="AC159U_04345"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3336320.1"
                     /translation="MTISEYKKFFASINSYPKKSLGQNYLIDKNILYKIGEIAELSPD
                     DQIIEVGTGFGFLTTFLAERVSRVVSIEKDKTVFSHISKKLSESNNIELINQDALDIK
                     FKDIVGDKKYKFVSNLPYSVASQIIFGLLECADSFANLVIMVQKEMGERICSPPACKQ
                     YGAFSVIIQSYFNTEIKHKVSPNSFWPKPEVDSVIIKMTPRKEKIVSDTLRPLFNEVV
                     KKSFQTRRKKLINNLKSILEQNALLDIFAELDIDEKSRAEQLSLNNYVDLSRAMKSKL
                     D"
     gene            complement(12217..12486)
                     /locus_tag="AC159U_04350"
     CDS             complement(12217..12486)
                     /locus_tag="AC159U_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR3336321.1"
                     /translation="MVINIIRISIILCLTIVFSFLFIKEISKLEALKHENEKIITQGA
                     ALEKENELLKIKIEALKNDQEYIEVIARQELGMIKEGEKIIKFTK"
     gene            complement(12487..13131)
                     /locus_tag="AC159U_04355"
     CDS             complement(12487..13131)
                     /locus_tag="AC159U_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3336322.1"
                     /translation="MIIECIVGGSFLTNCYIIADEESKQGIIIDPGAQVGQVIQRVEE
                     LGLTIDKIVNTHAHIDHASGVQEAKDALGAKFYLHPKEKPVLDLLPESALRFPEFSGV
                     EVPEIDEYLEEGDVVEIGKYRADVLLVPGHTWGSICLVVDEHIFSGDTLFAGSVGRVD
                     LTGGTSMQELVGSINKKLMVYPPESKVYPGHGPVTTIDIEKRSNPFLGGGTLLF"
     gene            complement(13153..>13415)
                     /locus_tag="AC159U_04360"
     CDS             complement(13153..>13415)
                     /locus_tag="AC159U_04360"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3336323.1"
                     /translation="HLIVHGIVQGVNFRFSTQQKAQSLGLVGWVRNKKDGTVEAVAEG
                     SDEEISKFISWCNTGPKMAKVTKVDITEVASSSKLADFTIEF"
BASE COUNT         4350 a         2555 c         2558 g         3952 t
ORIGIN      
        1 gccacacgat taaaaagcaa gatgttgcca tcactgtctg tggtaataac tccatcacct
       61 atcgacctaa gggtagttga aagcatctcc ttttcttcgg tgatggcatt ttccattttc
      121 tttctgccgc ttatatcctc aagaacaatc attcctcccg ttacttcatc attagaatcc
      181 ttggatggag aaagagaggc cataagccat aaatcctttc ttttgcctgc aattttaagg
      241 ttgaactcac agcgggaaaa actgcctttg agcatctttc tggatgcagc cgtaaatttt
      301 tcattaccaa gttctctaat atccattccg actatctcat caaaggttgt ctgaagaatt
      361 tctgcaattc ttctgttaca ctgataaacc ctaaaatcat tatccaaaag caaaacaccc
      421 accggggact tgtcaaaaag acttttaaaa cgatcttcgg attgttttag ttcctcattt
      481 ttgctaatgt agccaagagt catttcaatc tgtgaggaga ccgtatcaaa aaattctaaa
      541 tcctcttctg aaaaagcttc agtatcactt gttgaaaaca ggcttaatga accaattgtt
      601 attccatttt gttttaatgg aaccgagagg aagcttttaa caccaaactg cttttcagtg
      661 tctgtaatat atttactgtt atttacgtct ttaataaata ctctatcctc gcttattatt
      721 gcagcccaag gaagcccttt agaatagtta agagatgaag gaatatttat ggttttatca
      781 ccgggtcgag cagaacatat caacgccgcc ttttgatctt caagcttata tatggaaact
      841 aaatccacgc tgttcatacc gctttgcaaa ctggtagcta cagcatttaa aatttcagta
      901 aagttgttag aactgtacgt tgcatttaat acagatttga gtagtgattt atgatcgttt
      961 gggctctgtt ctactttggg agctgtatca ttgttttcgt tttgtaatac atctgactta
     1021 acttcctctt tgtttactgt agatgtaaag aacccaatat gattttcaga attgcttgtt
     1081 tctttagttt tctgcttact agattcactt ttattttcat ctattaatga atttataccg
     1141 tctctgagct gtgttttctt aaaaggctct ataatgcata tatgtttaaa atctaatttc
     1201 tctttaataa agttttcggc agtgagttta tcggtaataa acaaaaaact agtctttaaa
     1261 ttagtaaaaa tttcatttgc aagatcaaga cattttttat aattttcctt acttgcatta
     1321 aacaggatta accccggagt taattcagcc gctttatcca aaaatatttc atgatctgta
     1381 acaactgttg ttggaaatcc aagctctttt acaattgagt ttagataatt ggctatttta
     1441 gagttctgtt cataaataag tacagtccta tcaagcatta caatgttctc ctgatacagt
     1501 aagcccttac aacttcataa ttatacatct atttttattt agctgttgta aaatattgtt
     1561 gcaattaaaa tgccaattgg aatgttagga aatagcttaa gtatctgaag tttaatagta
     1621 tcaaggcatt ttagatactt gagaaattag aggtatacac tggtaaatat tgagttagct
     1681 aagcctattt acagcctctt ttaaaggagt ttctacaata tttctaattt ctttgagtct
     1741 gttctcatct tgtgcttcga atcttaacac tactgcaggc tgtgtatttg atgctctaag
     1801 gagtccccat ccatcaggga attcaatcct taaaccatcg atcccgttta cctcatactg
     1861 gctggaaagc tcttttttta cttcatccac tgccttgaat ttaatatcat cagggcagtc
     1921 aacccttatc tcaggagtgg ataatgcctc aggcacgcct gagagtaagt ctgaaaccgt
     1981 tttgcctgtt ttggataata tttcgagcac tctaagcgcc gcatatacag catcatcata
     2041 tccaaaaaac ctgtgtgaaa aaaatatatg cccgctcatc tccccggcaa gctctgcacc
     2101 ttgttccttc atcttgcttt ttataagaga atgccctgtt ttccacataa ggggtttacc
     2161 gccgctttgc tcaatagcat taaaaagcct gccagagcat tttacatccc ctattatctg
     2221 agcccccggg acttctttta aaacatctct tgcaagaata agcatgagca tatcgcccca
     2281 gacgatgtta ccattttcgt ctactacacc tattctgtcc gcatcaccgt caaatccaat
     2341 accgatatca agacaatttt ccttaactac cttaataagg tcctgcagat tttcttcaac
     2401 agttggatca gggtgatgat ttggaaaatt tccatcaggt tttgtgtaaa gctcagtaac
     2461 ttcacaccct tttcgtcgga taatatctgg acctacaaga ccacccatgc cgtttccaca
     2521 gtcaatccca actttaagtc cgggtttaat atcaatgttc tcagtcaggt agtttatata
     2581 taaatccatt atatcagtct tctcagtttt gccgtttcct gctgcaaatt ccccactctc
     2641 ggcgatttgc cttatttcct gaatctggct tccataaagg gattctcttc cgatgcaaag
     2701 cttaatacca ttaaagtcac tgggattatg acttgctgta atcatagcac cgcctccaac
     2761 ctccagatta aaaagggaaa aatagaccat tggtgtagat accattccaa gatcaactac
     2821 gtcaatgcca caggagttaa gccccttgca aaatgtttgc gaatataatg gggaactctc
     2881 tctgcagtcc cttcctaagg aaacaatttt tttgccatga ttggatagat acgtcccata
     2941 agcctttgct attatttcaa ctatttgttc tgatagatct ttatcaacta ttccccttat
     3001 gtcatattct ctgaaaattc cagggttaat ctgtagcatt tgtacatcct ccaattttac
     3061 atctgtaatt atttgaaaaa taagtaatca gttaagaata tatgattgca aggattatta
     3121 ctgattatat ttaataaata aacatcttta cacaaaagcc tatccaatta aattttatat
     3181 actcaattat aagcatgctt gactaaacac tctgcgcttc ttattctttg agccatgatt
     3241 tttcctactt ttacagagtt taagaaaaaa ctggataagg gaaatcttgt gcccgtttgg
     3301 agtgagtttc ttgctgattt tgatactccg gtatcagcac taaaaaagat agataagggt
     3361 aaatactcat ttttgtttga aagtgttgag ggtggagaaa aatggggtag gtacagtttt
     3421 ttgggaacgg aacccacagt tattttccgc tcaaagggaa acaaaattga aataatagaa
     3481 aacggaaaga ctaaaacaga acagggcaac ccgattgacc gattaagggt gctgatgtca
     3541 aaatataaac cagtccctgc ttcagagctg ccccggtttt caggcggggc ggtagggttt
     3601 ttttcttatg acattgtaag gtttgttgaa gacctgccgg tagatacaac tgacgacctt
     3661 ggctgctggg actgtctata tatgattaca gataccattt tggtttttga taatgtaagc
     3721 cataaaatca aaatcatctc taatgccttt gctgaaaaca agaaagatgc caaagaagct
     3781 tatgataatg caattaaaaa aataggtgaa ctaaaatcaa ggatatcaaa gaaagcctca
     3841 ttctatgata accaggcaac ggcaaagaaa cgtagcaaaa gcaagcctgc tataaaatca
     3901 aatttcaagc cccaagacta taaagaggct gtaaacaaga ctaaaaaata tataagagaa
     3961 ggcgatataa ttcaggccgt aatttcccaa aggtggaaaa caaagcttaa caccgacccg
     4021 cttgacctgt atagggctct taggatattg aacccttcac catatctttt ttatttaaaa
     4081 atgggaacgg agtatctggt aggctcatca cccgaagtaa tggtaagagt agagggtagt
     4141 aatgtagaga acaggcctat tgcaggcacg cgcccaaggg gaaagaacga atctcaagac
     4201 aacaaatatg aaaaagagct tcttgcagac cctaaagaga gggcagagca cataatgctt
     4261 gttgatcttg ctagaaacga ccttggacga gtcgcaaagg taggcacggt aaaagtggat
     4321 gaatttatga ctgtcgaaag atactctcat gttatgcata tagtgtctaa tgtgattgca
     4381 aagctaaaaa gaggcaaaga tgcttttgat gtaattaagg ctacttaccc tgctggcaca
     4441 ctctcaggcg caccaaaagt aagagcaatg gaaattatag aagagatgga atctactaga
     4501 agagggccat acggaggggc agttggttac ttcagttttt ccggcaacat ggatacttct
     4561 attgtaataa ggacatttta cataaaggac ggcagcattt attttcaggc aggcgctggt
     4621 atagtagctg actcagttcc tcaaaatgag tacaacgaat gcgtaaataa agccaaggct
     4681 ttagtaaaag caattgagct tactgagagc ggcttataga attaacagaa taaaaacaaa
     4741 tgattttaat gctcgacaac tatgattctt ttacatacaa ccttgtccac tatcttggtg
     4801 aacttggtca agatgtagtg gtacaccgaa atgataaaat cacagtagaa caaatcgaac
     4861 aggccgaccc cgatataatc atgatatcac ccggcccatg cacgccaaaa gaagccggga
     4921 tttctatgga tctggttaac aagtttaaag gtgttaagcc catatttgga gtgtgtcttg
     4981 gtcatcagtc aattgctgaa gcatttgggg caaaaatcat caaggcaaaa aaactgctgc
     5041 acggaaagac ttcggaaatt tttcatgatg gcaaaggaat ttataaagga attgagaatc
     5101 cttttgtagc aacgagatat cattcgctta taatagataa aaaaaccctt ccaaaagatt
     5161 ttgaaatttc tgcctggaca aaggaaggcg aaataatggg tatcaggcac aaaaaactgc
     5221 aaattgaagg agtgcagttt caccccgaat ccatacttac tgaacgtggt aaggatctgc
     5281 ttaaaaatta tttagaaata caagagcagc ttagctaaga gcataaccaa taaaaaattt
     5341 ttaattatta ggagttaaga tgaagctatt atttagacct cgttacgaac aacatttagg
     5401 aaaaagggag ctgccatttg gcacttttga cgatccagct acctggcttc ccataacccg
     5461 ttatttagaa caaggggccg agaaatttcc aagaaaaaca ctattcaaag tagcagataa
     5521 cgatggagtc ttagtagagc aatattctta tgaacaggca aatggctggg caaacagagt
     5581 tgcaaacgcc ttgtcatcaa aatacaagat tcaaaaaggt gacagaatag gtatctacat
     5641 gctcaactgc tcagagttta tattctctat tcttggcata cataaggtag ggggagtaca
     5701 agtatctata aataaggacg aaaagggtga gcgactttct tatattatta attattccga
     5761 gatgaaagct ttacttgtag ataaatccag cctgcctttt ttacaggagg tttcagatca
     5821 gcttacaaat cttgaagtaa taataattac tgatgatcct caaaatatcc cttcaagaat
     5881 agcaaacata ccctgcataa gctttagtag ttttagctct ttttcaaagt tcaaccacaa
     5941 ccttctggtg aaaacctctg atgttgaaag gtgtatgttt acctcgggta ctacagggat
     6001 gccaaaggga gtgcttagag agcatggtgg cgtaatacta acggtaaggg gttatctgca
     6061 gcagcacgga gtaagaagcg gtgatacact catgagcgtg cttacgcttg cccatgcaaa
     6121 tgctcaggtt atgtgcctgt ttgcttcaat tgcagcaggg gcaacagctg tgttctaccc
     6181 acggttctct gcatcaagct tctggaaatg ggcctatgaa tgcggggcta ccgttaccaa
     6241 tatgcttgga gctgttgctg aatacctgtg ggcagcagag cctacagagt gggatagaaa
     6301 acacaacgta aggactgttc tttcaggccc tgcaccaaaa aacttagaag aatttcagag
     6361 tagatttaac acaagaacaa ttgacgggta cggctctaca gagatgggaa tggtactctg
     6421 gaaagaccca gaagaccata ggctcggttc atcagggtat ccgatgcagg gttattatgt
     6481 agaactacga aatccattaa atcacaatga agtcttaagc cccatgtggg accctaccga
     6541 agaccagact cctccagaag atgctcaggg gttattattc attagaccac ttatccctca
     6601 tactacacta aatgaatatt ttaaggacga gcaaagaacc aaggaagctt ttgatgaaga
     6661 aggatttttt aactcagatg acctctttgc aagaggcatg gacggaaggt attactttgt
     6721 gggcaggtat tcaagaataa gggtatccgg agagaatgtc gatcctgttg cagtcgcaaa
     6781 tgaagcactc aaatatgatt ccattcacga agctatagca gtcggtataa gactaccaaa
     6841 tgtagccgat gatgagatta agctcaatct cactattaaa cagggagctt catttgaccc
     6901 tgttgagttt tgtaaatgga tggcagaccg agtaattatt gccatggttc caagatttat
     6961 tgaagtctat gagagcggtt ttccaacgac agccacacag aaagtaagag tagcggagct
     7021 aaaagaactt tccgaaaaga cctgggacag aaatgaatca gggcttaaat ttagcgccag
     7081 aaaataatgc cgggaaaaaa atcttcttta cattagaact ttgctgttat tattatcagc
     7141 aactaaataa cgatccattc agtaccaact atggctcaaa aagatactgt ttatctgatt
     7201 gacggaagct cctatatatt tcgtgcatac cacgctataa gaatcctttc aaactccaaa
     7261 ggatttccca caaatgcaat ttatggcttt accaacatgc tccttaagtt tctaaaagac
     7321 tacgacccaa aatatatagg cattgtattc gattctaagg gagataattt tagaaatgaa
     7381 atttatccag actataaagc caatagaggt gagccgcccg acgacctcaa accacagttt
     7441 gaagagatat ttggtctggt taaagctttt aatatgccca tggttcttat ggaggggtac
     7501 gaagcagatg acattatagg cacccttgca aaaaggtttg agagtgaaaa tatggacgtg
     7561 gtaatagtaa cgggagataa agacttcacg cagatagttg atgactctat aacacttctg
     7621 gatacaatga aggataaggt cacagagact aaggatgtgg tagaaaaata tggagtggag
     7681 cctgataaaa taatagaaat atttgctcta accggtgaca gtattgataa tattcctgga
     7741 gtaaggggaa taggaggaaa aacagccaca acacttattc agcaatacgg gtcgcttgaa
     7801 aatgtgtaca ataacctcga taaattaaag ccaaggcaaa aagagctttt ggaaaattct
     7861 aaagaaaatg cacttcttag cagagagctt gtaacaatca aatctgatat agaaatggat
     7921 attgatctgg atgaatatga atacacgggt tttaataaag aaaaattaat agaacttttt
     7981 aagcaatatg agttttcatc tctgctaaaa gaactcgata ccgacggcaa agaaaaaatc
     8041 cagaccaatc agcaaaccaa ctctgcaaac aaatcagata acagcgtaac ttatgataac
     8101 tataatttaa taaccaatga aaaagatttt gaaaaagtca tagatctgat aaataagacc
     8161 aagacactat ctatcgactt ggaaacaact tctcagcttc caatggaagc agaaattgta
     8221 gggtttgctc tctgccctcg gccgcaccaa tcttactatg tgcctgtagg gcacagaaat
     8281 cttttggatg caaaaaacca attgtcactg caatatgtgt gtgacagatt aaaaccaata
     8341 attgaagatg aacagataaa gaaaattggt cagaatctga aatacgaata tgtagttttg
     8401 tccaaatatg atatagaact taagggtatc tattttgata ccatgatagc agctcaccta
     8461 atagattcat caaggcttag ctacagcctg gatgagcttt caaaaaatta tctaaaccat
     8521 aagccaataa gctataaaga tgttactacc gagggcagat tcaaaatcac gtttgaagaa
     8581 gtggacattg agcttgcaaa aacatacgct tgcgaagatg ccgatgtagc atatttactg
     8641 agtaaaatat tgttagaaaa actaaaagag cttgatgtat tagaggttta taacaaatat
     8701 cttttagaat taatagtagc cctggcggga gttgaaatct caggggtaag gattgataca
     8761 gacaagctaa accatttatc aaaagagttt gaaaaaatca ttttagatac tgaagagaag
     8821 gtctacaaag aatcaggcac caaattcaac ctaaattcaa cacaacagct tcgcgaagtg
     8881 ctttttgata agcttgggct tcctgccaag aaaaaaacca aaaaagggga gccatcaact
     8941 gatgttgaag tactcactga tctaagcaaa gatcatattg ttccaaaaat gatgcttgag
     9001 tacagagcac ttacaaagct aaaatcaaca tatattgact ccctgccaaa actaataaac
     9061 agcaaaaccg gcaggattca tacatctttt aatcaggtag gaacatcaac gggaagagta
     9121 agctcaagcg acccaaacct acagaatatc ccgataaggt cagaagaagg aatgaagatt
     9181 agagaggcat ttgttgcgga aaaggattat aaaatacttt ctgctgatta ctctcagata
     9241 gagcttagat tactagccca tttttctgaa gacaaaaacc tgctaagtgc atttatttca
     9301 ggaagcgata tacacaacag aaccgcatcg gaaatatttg gtgttaaaca agaggatgta
     9361 actgcggata tgagaaggct tgcaaaaaat attaatttcg gaattattta tggaataagc
     9421 ccttttggtc tagcaaagca gattggcacc gccgttaatc aggcaaaaga ttatattgat
     9481 cagtatttca gcagatatag cagcgtaaag cagtatctgg atgattcagt aagaaatgct
     9541 caggagcttg gctatgccga aaccctaatc ggcaggagac gttatatccc cgagcttagg
     9601 tcaagcgacc ggtcagccag aggttttgca gagagggctg ccattaatac acctattcag
     9661 ggatccgcag ctgatgtaat aaatacagct atgattaata taaataaaga gataagaaat
     9721 aattataagt caaagatgat tttacaggtg catgatgagc tgctctttga agttcatgag
     9781 gatgaaataa aggcttttac agagtttatt aaaacaaaaa tggaaggtgc atggaaacta
     9841 aaagtgccgc ttatagttga tattaatatt ggaaattcct gggccgaggc tcactagtac
     9901 aatttatcta tatttatgcg atcaaaatat ttaatttatc taacacttgg aatatttata
     9961 tcccttgtat gttataaaag ctctgatgca ggatacagtg aatattcaac aaggcttcat
    10021 aatataattc ccgcaagcgc aaaaaagaat aataagcttg gcgtagttgt taaatctctt
    10081 ataaacaaaa aaaagatttt tgagtacaac tcggatacgc tttttattcc ggcttctaat
    10141 caaaaggtaa tagtttctac agttgcactt tcccttttaa aatccaagtt cagattcaaa
    10201 acagaatttt acgcaggcgg tgatataaaa agcggagtgc tccatggcgg gctgtatata
    10261 aagggctatg ctgatcccac tatcaacact gaaacactca gactcatagc catgcagttc
    10321 aagcaaaaag ggcttaagca gataaatgga gacatttatc tcgatgattc atattttgat
    10381 gacctaaact atcccgtagg ctggaagagt gaatggaggg atgattttta tagcccacct
    10441 ataagtgccc tgtctttaaa ctataataac atagaagtcg ttgcatatcc ttcaaaaaac
    10501 agatcgaagg ttgttgttac cacagatccc aaagtcccgg ggcttaagat tataaattca
    10561 gcttatctta aaaagaaaaa aaacagtatt gtagcaagat gggccgatgg taaagaacag
    10621 attatagtct cggggagatt atatacatac agcaagggtg taagactaaa gcttccagta
    10681 aaatatcctt cactttattt tggaagggta tttaaaaatg ggcttgctga gcagggaatc
    10741 actattacag gacaggttaa atttgccaat gttccaagat gggccaatct gtatcacaaa
    10801 tatgagtcag accgtcttga tgaagtggta aatgaatata ataaaaaaag cgtaaatata
    10861 attggagaga acttgatcaa gacacttggg gcagaatata ttggtactcc cggctcttgg
    10921 gagggtggaa gaaaagtcat ctctcttttt ttaaaaaata tcggcattaa agatggcttt
    10981 aagatagtag acggctccgg cctctcgctt ctgaacagag tctctcccaa tgcccttgcc
    11041 gatgttcttg aatatgcatt tcagaataag agcgtagggc ttgattttat tacttcgctg
    11101 cctgtagccg gaatcgatgg aactctcaga aaaagattta aggataagga tgtaaaaggc
    11161 agaattgtgg caaaaacagg ctatctcaag aacgtaagat cactttccgg gtatgtattt
    11221 acaaccacag gtgatgtgct tgtattttcc attctctcta acggactagg tcacgaagcc
    11281 aagaaatttc agagtgacct gctggcagag cttgtgagct gctgtgaggg ctaatcaagt
    11341 ttacttttca ttgctctgct caaatcaaca taattattta aacttagctg ttcagccctg
    11401 gatttttcat caatatctaa ctcagcaaaa atatccagca aagcgttttg ctctaatatt
    11461 gatttcaaat tattaataag tttcttcctt cttgtctgaa atgatttttt tactacttca
    11521 ttgaaaagcg gcctcaaagt atcagagact atcttttctt ttcgaggagt cattttaatt
    11581 attaccgagt caacctcggg ttttggccag aaggagtttg gagaaacttt atgctttatt
    11641 tcggtattaa aatatgactg tattattacc gaaaaagctc cgtactgctt acaagctgga
    11701 ggagagcaga ttctttcgcc catctctttt tgaaccatta ttactaaatt ggcaaatgaa
    11761 tcagcacatt ccagaagacc aaaaattatc tgtgaagcaa cgctgtacgg caggttggat
    11821 acaaatttat attttttgtc ccctacaatg tctttgaact taatatcaag ggcatcttga
    11881 tttatgagtt caatattatt agattcactt agctttttac ttatgtgact gaaaacggtt
    11941 ttatcttttt caattgaaac aaccctgcta acccgctctg ccaaaaaagt cgtcaaaaac
    12001 ccaaaccctg tccctacttc tatgatctgg tcatccggac ttaactctgc tatttctcca
    12061 attttataaa gaatgttttt gtctattaag tagttctgtc cgaggctttt ttttggataa
    12121 gagtttatgg atgcaaagaa cttcttatat tctgatatgg tcataaagaa aggttatacc
    12181 agatgggaaa acaataaaga aaaatgccag gcaggattac tttgtaaact taattatttt
    12241 ttccccttct tttatcattc caagttcctg gcgggcaatt acctcaatat attcctgatc
    12301 atttttcagc gcttcaattt ttattttcag cagctcgttc tctttttcga gtgcggcacc
    12361 ctgtgtaata attttttcat tttcatgctt taaggcctca agctttgata tctctttaat
    12421 aaataaaaat gaaaaaacta tagttaaaca caggatgata cttattctta tgatattaat
    12481 aaccatttag aaaagtaatg ttcctccgcc taaaaagggg tttgatcttt tttcaatatc
    12541 aatggtggta acaggaccgt ggcccggata caccttgctc tcaggtggat agaccataag
    12601 ttttttgtta attgaaccta caagctcctg catggatgtg cctccagtga ggtccaccct
    12661 gccgacactt ccggcaaaaa gtgtgtctcc tgagaatatg tgctcgtcta ctacaagaca
    12721 gatgcttccc catgtgtgac ctggaacaag gagtacatct gctctatatt ttccgatttc
    12781 cactacatca ccctcttcga gatactcgtc aatctctgga acttctaccc ctgaaaattc
    12841 cggaaacctt agagctgatt ccggcagcaa atccaaaacc ggtttttcct ttggatgcag
    12901 ataaaacttc gcaccaagcg catcttttgc ttcctgtact cccgatgcat ggtcgatatg
    12961 cgcatgagta tttacaatct tatcgattgt taatcccagc tcctctaccc tctgaattac
    13021 ctgcccaacc tgagcgcccg gatcaattat gattccttgt ttagactctt cgtcagcaat
    13081 gatgtagcag ttagtaagaa agcttccgcc aacaatacat tctataatca tagtttaatt
    13141 aatttaaata atttagaatt caatagtaaa atctgcaagt ttacttgaag aagcaacctc
    13201 tgtaatatcc acttttgtga cttttgccat ttttggaccg gtgttgcacc agcttatgaa
    13261 cttgcttatt tcctcatcac tcccctcagc aactgcctcg acagtgccat cttttttgtt
    13321 cctcacccag cctacaagac ccagactctg ggctttttgc tgagtagaaa aacggaaatt
    13381 cactccctga acaatgccgt gaacgattag gtgta
//
LOCUS       DBNGSV010000125         3590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1201536, whole genome shotgun sequence.
ACCESSION   DBNGSV010000125 DBNGSV010000000
VERSION     DBNGSV010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1201536"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..959)
                     /locus_tag="AC159U_04365"
     CDS             complement(6..959)
                     /locus_tag="AC159U_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336324.1"
                     /translation="MVLPSAELFVDKDGVYRVTYEDLQGAGLDLFGVKTSEIALTNSG
                     APVPLYIHTSSGKTFGPGGYIEFYGQALDTLYTNTNVYVLSVNADLAQSVDVDKKQAG
                     KNTAVATSYLETRTVDNNNSYSFSSPNGDPWYDVRLFARTSLLTRDFNIELDNVASGN
                     GGSINVSYYGLTDFPENPDHHVQTVFNGTQISDDIFDGTTNIEINEDLDSHGVQITEG
                     TNTLTLNLPGDTGVAFDIVNFDSFSVTYPIAFVANNGKLEFSSAASAYEVTGLDTENV
                     VVYQIANDGTVTRREGISTTQTPDGYMTLPHIIVPDYKLEF"
     gene            complement(995..2272)
                     /locus_tag="AC159U_04370"
     CDS             complement(995..2272)
                     /locus_tag="AC159U_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336325.1"
                     /translation="MNFFIYLRVGLLAATLCLLFSSDSFAGVPDPNWVAQTTQADCTG
                     AGAQKPGANPGPPDDVCQSYDQDLYENLEATGNGFCDSDIANYTVGGDPDFYYFEWDM
                     VGDWNNCNGDMVGEGHNYYIAIEIIRDNTGTNVSDNRPDYYIESHYKQGDHVDSGNLN
                     NWIAEFENNVKAFIDTDMPPDAGGPNPLTSDNPSPADGMDTELQTSDSDDAYLRISTN
                     FNPQLAIRASLIGNPTEIRTRAIVFQSSSLGGDKFPWHDHQPTSDLPGQNFDTVCAIG
                     SNCLNSTPITLSYFNAESAGGSVNFSWSTGTEAGNAGFNLYAQGDGGKEKLNTEIIQS
                     SVGDSLVRTDYSYSAESGAEIFYISDIDILGKETFQGPFTINTEYGSREAADPIKWAE
                     IKKESSGKQEIREVIKKSKAKKQIKAITHSRAY"
     regulatory      complement(2331..2428)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2551..3258)
                     /locus_tag="AC159U_04375"
     CDS             complement(2551..3258)
                     /locus_tag="AC159U_04375"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3336326.1"
                     /translation="MSDYLDSQIISATITLDSKIVEKFVLKPDITAVGRAKDNDIVID
                     NPSVSRYHLFIQKKDGKVKLKDLGSGNGTYVNGVKVEEAIIKPDDLILIGKFILKIEV
                     DHDDTLRKQEQYADRHDGTVIYDTGAQDKYVSNVKHKAQSQGAMLRMLGGEEVLLDKN
                     VFTIGKGADADIEIKGLFTSEHQAKIVRLEDGTFRIFNMGNFLNPTKVNGRPVTDQLL
                     HDYDVIEAGTSKFIFLD"
     gene            complement(3441..>3590)
                     /locus_tag="AC159U_04380"
     CDS             complement(3441..>3590)
                     /locus_tag="AC159U_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3336327.1"
                     /translation="WVNYEGIQSDIFDHILAIVPEFGLKVFQNPAGSDFKAIANTDKE
                     INKHS"
BASE COUNT          920 a          788 c          650 g         1232 t
ORIGIN      
        1 attacctaaa attctaactt ataatctggt actattatat ggggcaaggt catgtatcca
       61 tccggagtct gagtggtgct tataccctct ctccttgtta ctgtaccatc atttgcaatc
      121 tggtacacaa ccacattttc tgtatcaagt cctgttactt cgtatgcgct tgctgctgaa
      181 ctgaactcaa gttttccgtt atttgctaca aacgctattg gataggtaac actaaaacta
      241 tcgaagttta ctatatcaaa cgctacccct gtgtctccgg gaaggtttag tgtaagggta
      301 ttggttcctt ctgttatctg tactccatga gaatctaaat cttcatttat ctctatattt
      361 gttgttccat caaatatatc atccgatatc tgagttccgt taaacactgt ctgcacgtga
      421 tgatcagggt tttcgggaaa gtctgtaaga ccgtaataac ttacgtttat actgcctcca
      481 tttccggatg ctacgttatc aagctctata ttgaagtctc ttgtaagtag gcttgttctt
      541 gcaaacagcc gtacgtcata ccacgggtct ccatttgggg agctaaagct gtagctgtta
      601 ttattatcta cagttcttgt ctccagataa ctagttgcta ctgctgtgtt tttacctgcc
      661 tgttttttgt ctacatctac tgactgtgcc agatctgcgt ttacagaaag cacatacaca
      721 ttagtgtttg tatataaagt atctagtgct tgtccataaa actctatgta ccctcccggg
      781 ccaaatgtct tccctgaaga agtgtgtata tacaggggta caggcgctcc gctgttagtt
      841 agagctattt cacttgtctt tactccgaaa aggtcaaggc ctgcaccctg caagtcttca
      901 tacgttaccc ggtatactcc atccttatct acaaacagtt ctgcggaggg aagtaccacg
      961 ctccctccat tacctcctgc cgcggaggta gttgttagta tgccctgctg tgagttatag
     1021 cctttatctg tttctttgcc tttgatttct ttattacttc ccttatctct tgtttaccag
     1081 aactctcttt ttttatctct gcccatttta tagggtctgc agcttctctg cttccgtatt
     1141 ctgtattaat tgtaaaaggc ccttggaatg tctctttgcc aagaatgtct atatcgctta
     1201 tataaaatat ttccgctcca ctttctgcgc tatagctgta gtctgttcgg actagagaat
     1261 cccctacact gctctgtatt atctctgtgt ttagtttctc tttgcctcca tctccttgtg
     1321 cgtaaaggtt aaaaccggcg ttgccggctt cggtgccggt tgaccaggag aagtttacgc
     1381 ttccacctgc actctctgcg ttgaagtagg agagggtgat tggggttgaa tttaaacagt
     1441 ttgatccgat agcacatact gtatcaaaat tctgaccggg tagatctgat gtaggttgat
     1501 gatcatgcca tggaaactta tcacctccta aactactgct ttggaatact attgctctag
     1561 tcctgatttc agttggattt ccaataagac ttgcccttat tgcaagctga gggttgaaat
     1621 tggtgctaat tctcaaatat gcatcatctg agtcacttgt ttgcagttca gtatccattc
     1681 catcagccgg agaaggatta tcacttgtaa gcggattagg tcctccagca tctgggggca
     1741 tgtctgtgtc aataaatgcc tttacattgt tttcaaattc tgcaatccag ttatttagat
     1801 ttccactgtc aacgtgatca ccctgtttat aatgtgattc aatgtaatag tcaggtctgt
     1861 tatcacttac attggtaccg gtattatctc tgattatttc gatagcaata tagtagttat
     1921 gcccctctcc taccatatct ccattacagt tattccagtc accaaccata tcccattcga
     1981 aataatagaa atcgggatct cctcctacag tatagtttgc aatgtccgaa tcacagaagc
     2041 catttcctgt tgcttctagg ttttcatata gatcctggtc ataactctgg catacatcat
     2101 caggtggtcc gggattagca ccaggttttt gtgcacctgc tcctgtacaa tcggcttgag
     2161 ttgtttgggc aacccagttg ggatcaggaa cgcctgcaaa agaatcgctt gaaaacaata
     2221 aacataatgt agcggctaaa agccctactc ttaagtagat gaagaagttc aagttttacc
     2281 ccctatggta aacaattcta ctgtttaaac ctgtaaataa atgaacattc ttcggcagcc
     2341 attcggccat aggggctcaa ttacttaccg gcccccttag gtattgactt tgcgacccgt
     2401 attttcacac ggtgtgcctt ttcggagtaa aaactaacaa taccagtata ggacttttag
     2461 attatgaatg caagcagtat tattatcagg ttcaaattac ccgtgtgaag ttaaatatca
     2521 gcagaggaga atttttggaa atataagcta ttaatccaaa aaaataaatt tagacgtccc
     2581 agcttcaatc acatcataat catgtaaaag ctgatctgta acaggccttc cgtttacctt
     2641 ggtgggattt aggaaattgc ccatattaaa gattctgaat gtgccgtctt caagtcttac
     2701 tatctttgcc tggtgttcgc ttgtaaagag cccttttatc tcaatatcgg catctgcacc
     2761 cttaccgatg gtaaaaacgt ttttatccaa aagcacctct tctcctccaa gcatcctgag
     2821 cattgctccc tgagactggg ctttgtgttt tacattgctt acatatttgt cctgcgcacc
     2881 ggtatcgtaa ataacagtgc cgtcgtgcct gtccgcatac tgctcctgtt ttctaagtgt
     2941 atcatcatga tcaacttcaa tctttaatat gaattttcct ataagaatca ggtcatctgg
     3001 tttaattatt gcctcttcta ccttcacccc gtttacatag gtgccgttgc cgcttccgag
     3061 gtcttttaat ttcaccttgc catctttttt ctggataaac aggtggtatc gggaaactga
     3121 tggattatct attacaatat cattgtcctt tgcccttccg acagcagtga tatcaggttt
     3181 taatacaaat ttttcaacta ttttagaatc cagcgttatt gttgctgata ttatttgact
     3241 gtctaaataa tctgacataa ttatccacct ataaattaac tgtatttaag aatgataaag
     3301 aatagcccct atccttattt aacattcatt tttaactctt acacttaaac ctctgtactc
     3361 aatagcaaaa aaacaaaaga tttaaagcta ccttcctata aaacttatca aattttaggg
     3421 gtttttgcaa taagtaattt ttatgagtgt ttatttattt ctttatctgt gtttgctata
     3481 gccttaaaat cgcttccggc tgggttctgg aatactttta gaccgaattc ggggactatt
     3541 gcaaggatat gatcaaagat gtcactttgt attccctcat agttgaccca
//
LOCUS       DBNGSV010000126         1269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1894442, whole genome shotgun sequence.
ACCESSION   DBNGSV010000126 DBNGSV010000000
VERSION     DBNGSV010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1894442"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="AC159U_04385"
     CDS             <1..755
                     /locus_tag="AC159U_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336328.1"
                     /translation="SNLMTTALVDGDLIAYINAVSAIGAEEWVAKHRADKMLDRILGE
                     VQCDAYRVFISGGHNFRKKINPEYKSHRPPPPEHLEACQQMLINKWGAEVTDGFEADD
                     LLGIEQTDDTIICSIDKDLLMIAGKHYSWEITRKGKVSRPATFRTVEPFEGMIHFYKQ
                     MLIGDKADNLFGIHGIGEAKASKLLQDVPDEDLLKHIVMEMYIDDAVGGQQDRPDLEQ
                     EAVERFYMNADCFWIWRELGVTYSVREAMRED"
     gene            745..1032
                     /locus_tag="AC159U_04390"
     CDS             745..1032
                     /locus_tag="AC159U_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336329.1"
                     /translation="MKTNFYLLKPLMLDDSNWVHPERHIYESRITVMIDGELCSSVVH
                     TPGDLALEKMDDPNFRDFLMHKLKYNICRYITEKVTEGMQEVQTRILSGES"
     gene            1088..>1269
                     /locus_tag="AC159U_04395"
     CDS             1088..>1269
                     /locus_tag="AC159U_04395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR3336330.1"
                     /translation="MRRYPPKFQCLKEAKVGKKLNPASGRQAEHYKCAGCSEDFPQKE
                     VQVDHIKPVVDPRKGF"
BASE COUNT          429 a          183 c          307 g          350 t
ORIGIN      
        1 gaagtaacct aatgacaacc gccctagtgg atggagattt aatagcttat attaatgctg
       61 tatccgctat aggtgcggag gaatgggtag caaagcatcg tgctgataag atgcttgata
      121 gaatccttgg tgaggttcag tgtgatgcat acagggtatt tatttctgga gggcataact
      181 tccgaaagaa aattaaccct gaatataagt cgcacagacc tcctcctcca gaacaccttg
      241 aagcatgtca gcaaatgtta ataaacaaat ggggagcaga agttaccgat ggttttgaag
      301 cagacgattt actagggata gaacagacag atgatactat tatatgttct atagataaag
      361 acctattaat gattgctggt aaacattata gttgggagat tacaaggaag ggtaaagtat
      421 cacgccctgc aacattcaga acagtagaac cttttgaagg aatgatacat ttttataagc
      481 aaatgttgat aggggataag gcagataact tgttcgggat acatggcata ggagaagcaa
      541 aggcaagtaa acttttacaa gatgttcctg atgaagacct gttaaagcac attgttatgg
      601 aaatgtatat agatgatgct gtgggtggtc aacaagatag acctgattta gaacaggaag
      661 cagtagaaag attctatatg aatgctgatt gcttttggat ttggagagaa cttggagtaa
      721 cttattctgt acgggaggca atgcgtgaag actaattttt acttgttaaa accattaatg
      781 ttggatgata gtaattgggt acaccctgaa cgacatattt atgagtctag gattactgta
      841 atgatagatg gagaattatg ttctagtgtg gtacatactc caggagactt agcattagag
      901 aaaatggatg atcctaattt tagagatttt ttaatgcaca aattgaaata taatatctgc
      961 cgctacataa ctgaaaaagt tacagaagga atgcaagaag tccaaacgag gattcttagt
     1021 ggagagagtt aaaggttgga ctgagggtag attccgatct tttgtcttta gtcagatcag
     1081 ggcaggtatg cgtaggtatc ctcccaagtt tcaatgctta aaggaagcta aagtagggaa
     1141 gaaacttaat ccggcatcag gtagacaagc agagcattat aagtgtgctg ggtgtagtga
     1201 agactttcca caaaaagaag tacaagtaga ccatatcaaa ccagtggttg atccacggaa
     1261 agggtttat
//
LOCUS       DBNGSV010000127         8528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_855481, whole genome shotgun sequence.
ACCESSION   DBNGSV010000127 DBNGSV010000000
VERSION     DBNGSV010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_855481"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="AC159U_04400"
     CDS             complement(<1..253)
                     /locus_tag="AC159U_04400"
                     /inference="COORDINATES: protein motif:HMM:NF040082.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HIR3336331.1"
                     /translation="MSKDAEIKFTIQLDEQKVPKSIFWEATDSEFEEKKPCDSIMISM
                     WDRVAKNTLSIDLWTNDMEIGEMDAHFYFTFMKMAETYYK"
     gene            complement(318..2237)
                     /locus_tag="AC159U_04405"
     CDS             complement(318..2237)
                     /locus_tag="AC159U_04405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR3336332.1"
                     /translation="MIQLQDQKKRVLVVDDEHEILRIIYDLLSRNGYHVDTATSCEKA
                     LQLFGENEYNTILSDIKMPKKSGIDLLSEVSKKNPELPFVIMTGYASIETAIEGLKQG
                     AFDYLTKPLDYQKLKSIIVHAVENNQLKMERNRLLGELRELNSNLELKVKERNRELLN
                     IYSSTIESIITTDCDLNILTANPATTEIYGEQCVGKDLSDIMDGINFSTILPKILNNP
                     AYVTKHEIKKDDKFLKVSISQLVDFETNEIFGTIAVTEDITEKKKLEAQVIQSAKMSA
                     VGQLAAGIAHEFNNILTGIMGYTSIAMTRTNIEEINRDLHIVENASDRAADIVNKLLT
                     FSRTNEEKYHLVIVEDLIEDTLALIESAFESEGVNILRYFGKIPPVRMKPGEMQQVIL
                     NLAINAKHAMEGGGVITIQTEIEDEFVKIDFSDTGCGIPKENLPKIFEPFFTTKNDES
                     ELEGTGLGLSVVYAIIERHEGKIEVNTEVGKGTTFRIRIPNIQRIANGATSNLINVEP
                     RNINLNPKRKANVLVVDDDISVRNVLKEYLTLIGHNVVIINNGNDISEILETNHFDIV
                     FLNYTMPGKNGLDVLKEIKTYNESIMVVMITGKAPEEIRDDVMAEGAYSFINKPFTIN
                     QIHNTVARILGTEVN"
     gene            complement(2194..2874)
                     /locus_tag="AC159U_04410"
     CDS             complement(2194..2874)
                     /locus_tag="AC159U_04410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3336333.1"
                     /translation="MRNPLNIIGGARFYLSTEVDHGRNAEVVDEYLEFIKNQVVRTNR
                     LVNNLLNFSKPEEPSFKKVNLNSILENAIVLIKKQFTDSNIELEVDLDSDIPQVMGDQ
                     NQLWQVFINLLINATQCMEIGGKLHVTTSMSSKSPDCVEVNFKDTGVGISEENLTKIF
                     DPFFTTKTTGSGLGLAISYKIIVSHNGEIVVNSTEGEGTTFTIHLPINNENNHDTITG
                     SEEKSISS"
     gene            complement(2887..4737)
                     /locus_tag="AC159U_04415"
     CDS             complement(2887..4737)
                     /locus_tag="AC159U_04415"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3336334.1"
                     /translation="MNENNTSFSIFHGLVIILLTISVLLSLNAIVQVSKWKGNPFPGF
                     FLFNTVTVSQVSLPHWPSQDNDGPKSYDRIISVNNTKISNSQQVYEMISRYPVGTSIT
                     YLVERDDQKITLNIPTMEFKLFDLIQVFGVQFFAGVILLITGAIVYFLRPNIIESKAF
                     ILLCLSLGIWFIEDIDYQTTYSIIYSLNISYLALIFAPACWIFIALVFPNKKQIYLRN
                     KFILVVPFILSALIFVAHLKYLNNVRIIKLIDIIILFYMLISNILFLISIIQNYLKPE
                     SKLDQQRSQVVLLGSIFGFFIPTVACIIIILYGINYVTFLTIPVLFFPISIAYAIVKY
                     KLFDIEIIIQKTLVYTGLSGVLGFAIVIMILGFNYLFASQGGWKSPGFFVFLSVFLVV
                     ALNPVRDRIQNIIDGAFFRKRYDYERTIYNLGEAMTSILNIDEIGKKIINTITDTMQI
                     DSSTFLLLDREQSVYKVNVTTLEKLKSSTFNLDVEDKLISTLIQKRHEIFFEDLISDN
                     KYDDIKDELILTFNKLNSTLIMPLFFKDDLVGILSLGHKKSGLLFNSRDISLLKTLAN
                     QTAIAVENANAFRLVEDYASRLEETNKELIQTHLQLIQAEKMSAVGQLAA"
     gene            complement(4779..5504)
                     /locus_tag="AC159U_04420"
     CDS             complement(4779..5504)
                     /locus_tag="AC159U_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336335.1"
                     /translation="MITDAIQYDIDKYKIYDSIAPMLQKTMKQLKVNHIIDLCSGGSG
                     PLLRFYKMFESDDFPIKVTFTDKYPNTKSIQKIESYKDNINYIDRSIDAMDIPSNLKG
                     IRTMFTSFHHFPPEQAKLILQNAINNNSPIGIFEFTERKIVNLILSLLLTPILMFIVT
                     PFIKPFTFPRFFWTYIIPIVPILDTWDGFISNLRTYTPDELRNMVDSIGDNGFLWQIG
                     KIDSPMKPLSITYLIGYPKKFKN"
     gene            complement(5623..6651)
                     /locus_tag="AC159U_04425"
     CDS             complement(5623..6651)
                     /locus_tag="AC159U_04425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3336336.1"
                     /translation="MSNRVLVTGGTGFIGGNLTRKLVKKDYNVSALVRPTSNVENLNS
                     LGVELCYGDLSDTQSLKKATKDVEIVYHCAAKVSDWGKYEDFYEVNVEGTRLLLDSCL
                     ENKVKRFIYLSTTDTVWKYEHHINIDEDYPYPVKYKHPYCETKALSEKLLLNLINNDE
                     IDGGILRPCWVWGHGDNVVLPRLLELANDDKLFLVGKGDNKISICYVDNLTDAMIAMG
                     EIEKLKSNVYFVHDGYDITLYEFLKGVLNELQIDWTPKSIPYSIAYGCAYLAELYAKY
                     IINNCEPMLTRYVVSVLNRNLNYKISRACNELNYAPNLSFNDGLLKLRLWIDSIGGVS
                     ALLKSKLN"
     gene            complement(6696..8342)
                     /locus_tag="AC159U_04430"
     CDS             complement(6696..8342)
                     /locus_tag="AC159U_04430"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3336337.1"
                     /translation="MNQSSSDVSTVSIPFSGIDIFSKNIGKTLGCSGKLSNDGMFIET
                     DTVIEPNTKLLLEFKLPNTHFSIKTYSEVKNTGIKDSEQDNMLSGLKVKFVIIDKSDK
                     KRLEDFIESRVLKLNVDSDDYKLADFVNIPAKDIFEKTHKFWSYVEDWKEKGNYTLRK
                     HLLTASKNRLMLFDEVTGKKEEKINMGSGNYLGLSYHPEVIKAGEKALKKYGGSSVGA
                     PMHTGTYDVHVKLEKKLAEIKGCEEIMLFPSGYTANLGVISGLIMKDDVAILDRLAHR
                     SILDGVALSGATMRTFRHSDMNDLQRVLKSCESKYNGKVIIVDGVYSTEGDICPLPEI
                     VELGKKYDAKVMVDDAHAMGIIGENGRGTISHFGMEGEVDIVVGTMSKALGCVGGFVG
                     ASKEVINYLRHYGRSSVFSCNLPPQVAASALKSIEIMENEPEKIETLMKNVNYVREKL
                     EALGFNLLDFYSAIVVVIIGNDVIGKKMAAKILEEGIYLSYFPYPAVPVGKERLRLTV
                     MSTHTKEDLDKTIEILERVGKEYGVIDENSATDKNISSAA"
BASE COUNT         2653 a         1556 c         1227 g         3092 t
ORIGIN      
        1 atttataata agtctcagcc attttcataa acgtaaagta aaaatgcgcg tccatctcac
       61 caatttccat atcatttgtc cataaatcaa tactaagcgt gttcttagct accctatccc
      121 acattgaaat cataatagag tcacagggtt ttttctcctc aaactctgaa tccgtagctt
      181 cccagaatat gctcttaggc actttttgtt catctaattg aatagtaaat tttatctcgg
      241 catcctttga catatgcacc actccgcttt gatttgttta taatgaaatt ttaagtggaa
      301 aattagcaga ttaaatacta attaacctct gtaccaagta ttcttgctac agtattatga
      361 atttggttaa tcgtaaaagg tttgtttata aatgagtatg ctccttctgc cataacatca
      421 tcccttattt cttcaggagc ctttcctgta atcatcacta ccataatact ttcattatac
      481 gtttttattt ctttaagcac atctagcccg tttttaccag gcattgtgta atttagaaat
      541 acgatatcaa aatgattagt ttctaaaatt tccgatatat cattaccatt atttattata
      601 actacattgt gtccaatcag ggtaagatat tcttttaata cgtttctaac agagatatca
      661 tcatctacaa ctaagacatt tgcttttctt ttgggattta aatttatatt tctgggttca
      721 acatttatta gattacttgt agcgccgttt gcaatacgct gaatattcgg gattcttatt
      781 ctaaatgtag tgccttttcc gacctccgtg tttacttcta ttttgccttc atgtcgttca
      841 atgatggcat atactacaga aagtccaaga cctgttcctt cgagttccga ttcatcattt
      901 tttgttgtaa aaaatggctc aaatatcttt ggcaggtttt ctttcggtat tccacatcct
      961 gtatctgaaa aatctatttt aacaaattca tcttcaatct cagtctgtat tgtaatcact
     1021 ccaccacctt ccattgcatg ctttgcattt attgcaagat ttaaaattac ctgttgcatt
     1081 tcaccgggct tcatacgtac gggtggaatt ttgccaaaat atcttaaaat attgacccct
     1141 tctgattcga atgcgctttc tattagtgca agtgtgtctt ctattaaatc ttctacaata
     1201 acaaggtgat atttttcttc attggtcctg gaaaaagtta agagtttatt tactatatct
     1261 gctgctctgt cactggcatt ttctacgata tggagatcac ggtttatctc ttctatattc
     1321 gtacgggtca tagctatgct tgtataaccc ataattcctg tgagaatgtt gttgaattca
     1381 tgtgcaatac cggctgcaag ctgtcctaca gcagacattt tggcagactg aattacttga
     1441 gcttctagct tctttttctc agtaatgtct tcagtaacag ctattgttcc aaatatctca
     1501 ttggtttcaa aatcaaccag ctgagaaata ctgaccttta aaaatttatc atctttttta
     1561 atttcatgtt ttgtaacata agcgggatta tttaggattt tcggtaaaat agtggaaaag
     1621 ttaattccat ccattatatc cgacaggtct ttccccacac actgctcccc gtatatctct
     1681 gttgttgccg gattggctgt tagaatattt agatcacaat cagtagttat aatactctct
     1741 atagtactgc tatatatgtt aagcagttcc ctgtttcttt cttttacttt tagctcaaga
     1801 ttagagttta actctctaag ctctccaaga agtctgtttc tctccatttt aagctgattg
     1861 ttttctacag catgaacaat aatgctcttt agtttttgat aatccagagg cttggttaaa
     1921 taatcaaatg caccttgttt taacccttca atcgcagttt ctattgatgc ataccccgtc
     1981 ataataacaa acggcagctc gggatttttc ttgcttactt cagatagcag atcaataccg
     2041 ctctttttgg gcatcttaat atctgaaaga atagtgttgt attcattctc accaaaaagc
     2101 tgtaaagctt tctcgcaaga tgttgcagta tctacatgat atccgtttct ggatagcaga
     2161 tcgtaaatta tcctgaggat ttcatgctcg tcatcaacta ctaatactct tttcttctga
     2221 tcctgtaatt gtatcatgat tattctcgtt atttatagga aggtgaatcg taaaagttgt
     2281 cccctcacct tcagtgctgt taacaactat ttctccgttg tggcttacaa taatcttata
     2341 acttatagct agacccaagc cgcttccggt ggtttttgtg gtaaaaaaag gatcgaagat
     2401 ttttgtgagg ttttcttcac ttatccctac tcctgtatcc ttaaagttta cttcaacaca
     2461 atctggtgac ttgctgctca tgcttgttgt aacatgcaat ttgccaccta tctccataca
     2521 ttgtgtcgca ttaataagca ggttaataaa aacctgccaa agttgattct gatcacccat
     2581 cacctgtgga atgtcggaat ctaaatccac ttctaattca atattgctat cggtgaattg
     2641 cttcttaatt aatactatag cattttccaa tatgctgttc aaatttactt ttttaaaact
     2701 cggttcttct ggttttgaaa agttgagaag attgttaaca agtctgtttg tacgaactac
     2761 ctgattttta ataaattcca gatactcatc cacaacttcc gcatttctgc catgatcaac
     2821 ttcagtagac aagtaaaatc ttgctcctcc aatgatgtta agaggatttc tgatttcatg
     2881 ggcaagtcaa gctgcaagct ggcctactgc cgacattttt tcagcctgta ttaactgaag
     2941 atgtgtctgt ataagctcct tattggtctc ttcgagtctc gatgcatagt cctcaaccag
     3001 tctgaatgca ttggcatttt ctacagctat agcagtttga tttgcaagag ttttaagaag
     3061 gcttatgtct cttgaattaa ataaaaggcc tgatttctta tgtccaagtg atagtattcc
     3121 aacaagatcg tctttaaaga acagtggcat tataagggta gaattaagct tattaaatgt
     3181 aagaattaat tcgtctttaa tatcatcgta tttattgtct gaaataagat cttcaaaaaa
     3241 tatctcgtgt cttttttgta ttaatgtact aatgagttta tcttcaacat ccagattaaa
     3301 agtagaggat tttagcttct ctaatgttgt tacattaact ttataaacag attgttctct
     3361 atcgagcagt aaaaatgtgg atgaatctat ctgcatagtg tctgtaatag tatttattat
     3421 ttttttgcct atttcatcaa tatttaatat tgatgtcatt gcctcaccca aattatatat
     3481 agttctttca tagtcatacc gttttctaaa aaatgcgccg tctatgatat tttgaatcct
     3541 gtcccttaca gggtttagag ctacaactag aaatacgctt aagaaaacga aaaatccagg
     3601 acttttccaa cccccttgag aagcaaacag gtaattgaat cctaagatca ttataacaat
     3661 tgcaaagcca agcacaccgg aaagacctgt gtataccaat gttttctgta ttattatttc
     3721 aatatcaaat aacttgtatt taacgattgc ataagcaatt gaaataggaa agaataaaac
     3781 cggtatagtt aaaaaagtaa cataattaat accgtataaa ataataatga tacaagcaac
     3841 tgttggtatg aagaatccga atattgaacc taataaaaca acctgagatc tctgttgatc
     3901 taacttagat tcaggtttta agtagttttg aataatggat attaaaaata gaatgttact
     3961 tataagcatg taaaacagta ttattatatc tatcagttta ataattctaa cattatttag
     4021 atatttcaaa tgagcaacaa aaattagtgc agaaagtatg aacggaacaa ctaagataaa
     4081 tttattcctt aaatagattt gtttcttatt tggaaaaact aatgcaataa atatccagca
     4141 tgcaggagca aagattaatg ctagatagga gatattcaat gaatatatga ttgagtatgt
     4201 agtttgataa tctatatctt ctataaacca gatacctaat gataaacaca acaagataaa
     4261 tgccttactt tcaataatat ttggtcttaa aaaatacaca attgctcctg ttattagtag
     4321 aataactcca gcaaaaaatt gaacaccaaa tacttgtatc agatcaaaca acttaaattc
     4381 catagtagga atatttaatg ttattttttg atcatcacgt tctaccaaat aagtaatact
     4441 ggtacctact ggatatcttg atatcatttc atatacctgt tgagagtttg atatctttgt
     4501 attattaaca gatataattc tatcgtatga tttcggtcca tcattatcct gacttggcca
     4561 atgtggaagt gatacctgac ttactgtaac agtattgaaa agaaaaaatc cggggaatgg
     4621 attacctttc catttggaaa cctgaactat agcatttaaa gataataata cagaaatagt
     4681 taataaaatt attaccaatc cgtgaaaaat agaaaatgac gtattatttt cattcaattt
     4741 tggcataata tgttattaag taatttagtt ataaattttc aatttttaaa ttttttggga
     4801 tatccaatta agtatgtaat tgataacggc ttcataggtg aatctatttt acctatttgc
     4861 cataaaaatc cattatcacc aattgagtca accatatttc ttaattcatc gggtgtatat
     4921 gtcctaagat tggaaataaa accatcccaa gtatccaata ttggaactat tggaattata
     4981 taggtccaga aaaaccttgg aaaagtaaaa ggtttaataa acggtgtaac aataaacatt
     5041 aaaattgggg taagtaacaa agataaaatt aaattaacaa tttttctttc tgtaaattca
     5101 aaaataccaa ttgggctatt attattaatt gcattttgta atattaactt tgcttgttca
     5161 ggagggaaat ggtgaaaact tgtaaacatt gtgcgaatac cttttaggtt agatggaata
     5221 tccatagcgt ctatagatct atctatgtag ttaatgttat ctttataaga ttctatcttc
     5281 tgaattgatt tcgtattggg atatttgtct gtaaacgtaa cttttattgg aaaatcatca
     5341 ctttcaaaca ttttataaaa tcttagtaat ggtccagagc ctccagagca tagatctata
     5401 atgtgattga ccttgagttg cttcatagtt ttttgcagca taggcgctat agaatcataa
     5461 attttatact tatcaatatc atattgtatg gcatcagtaa tcatgtctct gaatattatt
     5521 ggtagccaag ctttatcttc aaattcaaat agttgtaacc ttaccattta tattttttta
     5581 tatatgattt catattgaat tttagtagtt atattattta catcaattaa gtttagattt
     5641 aagtaatgct gacacacctc caatagaatc tatccataat cttagtttta gaagaccatc
     5701 attaaaagaa agattaggcg cataattcag ttcattacaa gctcttgata ttttataatt
     5761 tagatttctg tttaagacag atactacgta acgtgttaac atcggttcac aattattgat
     5821 aatatatttt gcatatagtt cagctaaata agcacatcca taagctatag aataaggaat
     5881 ggatttaggg gtccagtcaa tctgaagttc atttaaaaca ccttttagaa attcatacaa
     5941 tgttatgtca tatccatcat gtacaaaata gacattactt tttaattttt caatttcacc
     6001 cattgcaatc atagcatctg ttaaattatc tacataacag attgaaattt tattatctcc
     6061 cttaccaaca aggaataatt tgtcatcatt cgctaattcg agtagtcttg gaagaacaac
     6121 attatctcca tgtccccaaa cccaacatgg cctaagaatt cctccatcga tttcatcatt
     6181 attgatcaaa ttcaacaaca atttttccga tagcgcttta gtttcacaat aggggtgttt
     6241 atatttaact ggataaggat agtcttcatc tatatttata tgatgttcgt atttccaaac
     6301 agtgtcggtt gtacttaaat atataaatct tttaacttta ttttctaagc atgaatcaag
     6361 cagcaaccgg gttccctcga cattaacctc ataaaaatct tcatatttgc cccaatctga
     6421 tactttagca gcacagtgat agacaatttc aacatcttta gtagcttttt ttaatgattg
     6481 agtatctgat aaatctccat aacataattc aacacccaga ctatttaaat tctctacatt
     6541 actagtaggt ctaacaagag ctgagacatt ataatctttt ttaacaagtt ttcttgttaa
     6601 atttccacca ataaatccag tccctccggt tactaatact ctattagaca ttcagattat
     6661 atttattgtt caagtcattt attagtcatt attttttaag cagcagaaga tatgttctta
     6721 tctgttgctg aattttcatc tattactcca tactcttttc ctactctttc aagaatttct
     6781 attgttttat ccagatcttc ttttgtatga gttgacataa cagtgagtct gagtctttct
     6841 ttacccaccg gaacggcagg ataagggaaa taactgagat atatcccctc ctccagaatt
     6901 tttgcagcca ttttcttacc tataacatca ttaccaataa taactacgac aattgccgaa
     6961 taaaaatcca gcaaattaaa tcccagggct tctagttttt ctcttacata atttacattt
     7021 ttcattaatg tttctatctt ttcaggctca ttttccataa tttctattga tttgagggct
     7081 gatgcagcaa cctgtggagg gagattgcat gaaaaaacgc tggatctacc ataatgtcta
     7141 agatagttaa ttacctcttt ggaagcacca acaaaaccgc ctacacaccc aagcgcctta
     7201 ctcatggttc ctactactat atcgacctcc ccttccattc caaaatgact tattgtaccc
     7261 cttccatttt ctccaattat tcccatggca tgagcatcat caaccataac cttagcatcg
     7321 tatttcttcc ccagttctac aatctctgga agcggacaaa tgtccccctc ggtactatat
     7381 accccgtcta ctattattac tttcccatta tatttagatt cacaactttt aagtactctt
     7441 tgcagatcat tcatatccga atgtctgaat gttctcattg tagctccgga aagtgctact
     7501 ccgtccaaaa tactcctatg tgcaagtctg tctaaaatag ctacatcatc tttcataatt
     7561 aaacctgaaa taacacccag gttagccgta taacctgatg ggaagagcat tatctcctca
     7621 caacctttta tttctgcaag ctttttttcc agttttacat ggacatcata ggtaccggta
     7681 tgcatgggtg cgcctactga agaaccaccg tactttttta atgctttttc accagcttta
     7741 atgacttcag gatggtatga aagaccaaga tagttaccac tgcccatatt tattttttct
     7801 tctttttttc ccgtaacctc atcaaacagc attagtctgt tttttgaagc agtaagcaga
     7861 tgttttctta atgtgtaatt acctttctct ttccagtctt ctacatatga ccagaattta
     7921 tgtgttttct caaatatgtc ctttgcaggt atgttaacaa agtctgctaa tttatagtca
     7981 tcagaatcta catttagttt taaaactctt gattcaataa agtcctcaag acgctttttg
     8041 tctgacttat caattatgac aaacttaact tttaagcctg acaacatatt atcctgctct
     8101 gaatccttta tcccggtatt cttaacttca ctatatgtct tgatgctgaa atgagtattg
     8161 ggtagtttaa attccaaaag aagtttagtg ttaggttcaa ttacagtatc tgtttctata
     8221 aacattccat catttgataa tttgccggaa catcctaatg tttttccaat gtttttagaa
     8281 aatatatcaa tacctgagaa tggtatggat actgttgaaa cgtcagaaga tgattggttc
     8341 ataatccctt ctccttaata aagtaaattc tttgatgtta atactacaga cctgttaatt
     8401 ttatattgta ttaaatttat gcaaacagat caactacttt tttgtgataa tatactttga
     8461 taagctatag ggccatgttt aaataatctg atatatgtaa catctaccaa ttttaggtca
     8521 gaatctgt
//
LOCUS       DBNGSV010000128         2546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1339786, whole genome shotgun sequence.
ACCESSION   DBNGSV010000128 DBNGSV010000000
VERSION     DBNGSV010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1339786"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="AC159U_04435"
     CDS             complement(<1..1609)
                     /locus_tag="AC159U_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical enzyme family protein"
                     /protein_id="HIR3336338.1"
                     /translation="MDYMASKVEVSDRVYKLLYHAILEQDIVPSMRLFMTAGIACERD
                     NIAAFNCAYVAINNKRAFSEILYILMNGTGVGFSNERHNICKLPQVPVLEQCDDVIVV
                     ADSKKGWAVAFRKYLSCLWEGDVPTIDYSKIRPAGERLKTFGGRASGMQPLKNLFVFC
                     YETFRAAQGRKLNSLEVHDIICMIGDIVVVGGVRRSALIGLSNLQDIRMREAKSGAWY
                     LKVEEGGTPWRMLANNSVSYTEKPDVKTFMEEWLTLVKSGSGERGIFNRVAAQNQASR
                     WGRRSADIDYGCNPCSEIILRDKQFCNLTEVIIRETDTVDELNRKIELATILGTYQST
                     LTDFKFLSEEWKKNTDEERLLGVSLTGIMDNKLTNGSAIGQQELLKDKLDFPTMLEGL
                     RDNARKTNEKWAKKWGISASTAITCVKPSGTVSQLVNSSSGIHARFAPYYIRRIQLDN
                     KDPVCQHLLESGFPLVPYEAKKETTMVASFPMKAPPNAIFRTDMSAIEQMELWLIYQN
                     HWCEHKPSCTVYVKEDEWPEVGAWVWQH"
     gene            complement(1717..1884)
                     /locus_tag="AC159U_04440"
     CDS             complement(1717..1884)
                     /locus_tag="AC159U_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336339.1"
                     /translation="MKDNPATDMFIEIVFKRMRSLGTPLNKAQEDELAKMINAFVESF
                     GNTIRSIYKAK"
     gene            complement(1874..2170)
                     /locus_tag="AC159U_04445"
     CDS             complement(1874..2170)
                     /locus_tag="AC159U_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017001697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HIR3336340.1"
                     /translation="MSEWHKGYQEVQNKLNEKAYKNAVAPASSKANMKQIFGTHYTNE
                     NIQPWDFIIANEIPYLEGNIIKYICRWRSKGGLTDLAKAQHYIEKLIEVNTDEG"
     gene            complement(2163..2402)
                     /locus_tag="AC159U_04450"
     CDS             complement(2163..2402)
                     /locus_tag="AC159U_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336341.1"
                     /translation="MKITWPDLWFAAINLWNAPHMTNSLTLNDIKDRLKTLDEVILLE
                     ILDISSEELVEMFSDRIEERKDYFIKDLEVEDWDE"
BASE COUNT          697 a          577 c          385 g          887 t
ORIGIN      
        1 aatgctgcca tacccatgcc ccaacttcag gccattcatc ttccttgaca tatactgtac
       61 aactaggttt atgttcacac caatggtttt gataaatgag ccacaattcc atctgttcaa
      121 tggctgacat atcggttcta aatattgcat taggtggagc tttcattgga aatgatgcga
      181 ccattgtagt ctctttctta gcctcataag gtactaaagg gaagccactt tctaaaagat
      241 gctgacacac aggatcttta ttatccaact gaatgcgcct gatataatat ggagcaaacc
      301 tagcatgaat accactacta gagttaacaa gctgagatac agtgccacta ggcttgacac
      361 aagtaatagc tgtagaagca gatatgcccc atttcttagc ccacttttca tttgttttac
      421 gagcattgtc tcttaatcct tctaacattg taggaaaatc cagtttatcc tttagtaatt
      481 cttgctgtcc gatagcacta ccattagtta acttattgtc catgatacca gttaaactaa
      541 cacctagaag tctttcctca tccgtgttct ttttccattc ttcagataga aatttgaaat
      601 ctgtgagtgt tgattgatac gttccgagaa ttgttgccaa ttcaattttt ctgttgagtt
      661 catcaactgt atctgtttct ctgataatga cttccgtaag gttgcagaac tgtttatctc
      721 ggagtataat ttccgagcat gggttacatc cgtaatcaat atctgcacta cgtcttcccc
      781 aacgagaggc ttgattttgt gcagcaaccc tgttaaagat tccccgttca ccagacccgc
      841 ttttaacgag agtgagccat tcttccataa aagttttaac atctggtttc tccgtatatg
      901 aaactgaatt gtttgccaac attctccaag gtgtaccacc ctcttcaacc ttgagatacc
      961 atgcgcctga cttagcttct ctcattctga tatcttgtag gttggatagt ccaattaaag
     1021 cactacgtct tacaccaccc acaaccacta tatctccaat catacatata atatcatgta
     1081 cttctaagct gttgagttta cgcccttgtg cagccctaaa tgtttcatag cagaatacaa
     1141 ataggttctt taagggttgc attcctgatg cacgacctcc aaaggtcttt aatctttctc
     1201 ctgctggtct tatcttacta taatcaatag taggtacatc cccttcccat agacaagaca
     1261 agtacttcct gaatgctaca gcccaaccct tcttactatc agcaactaca attacatcat
     1321 cacactgttc tagtactggt acttgtggta gcttgcaaat attatgtctt tcattagaga
     1381 accctacacc agtaccattc atcaatatgt atagaatttc actgaatgct cttttattat
     1441 taatggctac atatgcacag ttgaatgctg cgatgttatc acgttcgcaa gctatccctg
     1501 cggtcataaa cagacgcatt gatggtacaa tatcctgttc tagaattgca tggtataata
     1561 atttatatac ccgatcactt acctctactt tagaggccat gtaatccata tatctcttga
     1621 ccgtctcagg ccaagattct ctgcgtaaat cttcctcacg atacctagcg tatttagtaa
     1681 ggtgtattat atcttggtat gttgtaggta atgtattcat tttgctttat atatacttct
     1741 aattgtgttt ccaaaagatt ctacaaaagc attaatcatc tttgcaagtt catcctcttg
     1801 tgctttgttt aatggtgtac caagactcct cattctctta aatactatct ctatgaacat
     1861 atctgtagct ggattatcct tcatctgtat ttacctcaat taatttttct atgtaatgtt
     1921 gtgcttttgc caaatccgtc aatccaccct tgcttctcca tctacatata tactttataa
     1981 tattaccttc aaggtatggt atttcattgg caataataaa atcccaaggt tgaatgtttt
     2041 cattagtata gtgagtgccg aaaatctgct tcatattagc cttagaactt gccggagcta
     2101 ctgcattctt ataagccttt tcatttagtt tattctgaac ctcctgatag cctttatgcc
     2161 attcactcat cccaatcctc cacttccaag tctttaataa aataatcttt acgttcttcg
     2221 attctatcag agaacatttc aaccagttct tcagatgata tgtctaatat ttctaacaat
     2281 atcacttcat ctaaggtctt taaccgatcc ttaatgtcat ttagtgtgag tgaattagtc
     2341 atatgaggtg cattccagag gttgattgct gcaaaccata aatccggcca tgtaattttc
     2401 attcattcta tatcccttta tattcgatat taagtgactc tattttttct gttccctgta
     2461 accatttgcc acagttttgg cactgatatc tccaatactt cccagctaac cttattacct
     2521 tacctctgcg ctggaaatga cttcca
//
LOCUS       DBNGSV010000129         6230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1478947, whole genome shotgun sequence.
ACCESSION   DBNGSV010000129 DBNGSV010000000
VERSION     DBNGSV010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1478947"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="AC159U_04455"
     CDS             complement(<1..155)
                     /locus_tag="AC159U_04455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336342.1"
                     /translation="MPETKDLFENIAKVYDPLNTFFSMGFHKKWKTKLIKEFKGAQLL
                     LDLATGTS"
     gene            complement(219..1094)
                     /gene="metF"
                     /locus_tag="AC159U_04460"
     CDS             complement(219..1094)
                     /gene="metF"
                     /locus_tag="AC159U_04460"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR3336343.1"
                     /translation="MLFKEYYQVNDRPVISFEIFPPRTDEGLKKLKDVTLPELIGLNP
                     SYITVTYGAMGTTRDKTLDIASFIKNELSTETACHLTCVGSTRDDIDQILDNIAGNNI
                     HNIVALRGDPPKDGVVNENSYRYANELVAHIRDYERRNNLKFGIAVAGYPEKHLEAQS
                     IEDDIENLKKKINAGSDIIITQLFFLNQYYYEFIDITKSHGIDLPVIPGLMPILSTKQ
                     IQRISSMCGSKIPAYLKDDLQQANSDDTKAKKIGIDQCIIQANDLLSSGVPGIHFYVL
                     NHSEHIKEIISQLDI"
     gene            1262..2278
                     /locus_tag="AC159U_04465"
     CDS             1262..2278
                     /locus_tag="AC159U_04465"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR3336344.1"
                     /translation="MKIALILIAQFFFIFVPAYSDTTPKSYQKVQRAFYVMGTSLEFN
                     LLCKNRTHCLDAINESIVEVNRIDKIFSNYRADSELSAIRINKDQYIKVSREFFELTG
                     FSYFISALTNGAFDISVGPLVDIWKKEAKKNKIPKTSLIESIRDKCIGFDKLKLYSIH
                     KKIGFSSSCIKLDYGAVGKGHVLKKLAELLVSKKIENGLINFGGNIHALGKDLDGKPW
                     VIKIKDPDNSNKYIKTINVSNMSVSTSGGYERYFLVKDRKYSHIIDPRTGFPVSRFSS
                     VTVVSENPLFADALSTAFSVLSIEESKKIIDQLDKIAVLIIEKHENEINIYNNDNFNK
                     LVLN"
     gene            2618..4012
                     /locus_tag="AC159U_04470"
     CDS             2618..4012
                     /locus_tag="AC159U_04470"
                     /inference="COORDINATES: protein motif:HMM:NF023351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HIR3336345.1"
                     /translation="MDIQKGYKYLFFTIFILLFSVASSTVMSEEMDKDSRIRELERKL
                     EAVSQEVNKLKEQQGVSNKISEIEDKLTVLVEEIENIKSSSVTAERKYEQKHGHAEGA
                     SQVYHVGKGFSVGGYGELFISQIEEDDDNTVDTQRVILYVGHKFTDNIIFNSEIEFEH
                     GTTSSNQDGRSGSASVEFASIDFLISESFNIRGGLLLAPFGIVNEQHEPTTFFGVLRP
                     DIETQIIPTTWRESGAGIFGEINDVIPGTLSYRAYLMNSFDSRGFRASNNRSLRVKGN
                     RARFNDIAFVSRLEYDPIPGVKFGGSWFIGQTGQNESVDGQTIGGLFQMYEGDVDINW
                     RGFEGRALFVYTFLDDAELINAQNGLVGDDSVGEEQFGWYVQGAYNIMSLADVHPYFQ
                     YLAPFVRYEQYDTQKDVPAGFLRNPANDRDVLTFGLNYKPIPNVVVKVDYQVRDDEAD
                     SSNNQINLGLGYVF"
     gene            4002..4583
                     /locus_tag="AC159U_04475"
     CDS             4002..4583
                     /locus_tag="AC159U_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR3336346.1"
                     /translation="MCFKLLSNKIIREAGKLISASLIINILLISPNLYAKVFMPKDEA
                     LKVAFPNADTIEKKSYFLKPEQINDVEELSKTKVGSKLFLLYIGKKDGRDVGYAAIDT
                     HTVRTKTQTIIVVINPDGSLNYTEILAFFEPQEYKPTEGWIDLFKERKLSDGIKIGYD
                     IPNISGATLSSNATANAVKKVLAIFEVVIRGAN"
     gene            4586..5080
                     /locus_tag="AC159U_04480"
     CDS             4586..5080
                     /locus_tag="AC159U_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336347.1"
                     /translation="MRYFISNDINKNDLLKIIIGFTILFFVFLWITNLLLYLQIGFSY
                     ESVVQYYLGSEESFRPAKSYIGLLQESHFHFFSMAILLVTLNHLILFASIKNIYKLIL
                     ILSSFLSALGDISAGWLIRYVSPYFSYLKIVSVIVLQISLALLIIVLIIFIYNKGKKA
                     LYTG"
     gene            complement(5087..>6230)
                     /locus_tag="AC159U_04485"
     CDS             complement(5087..>6230)
                     /locus_tag="AC159U_04485"
                     /inference="COORDINATES: protein motif:HMM:NF052799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7986 domain-containing protein"
                     /protein_id="HIR3336348.1"
                     /translation="NLLPEDLAMDEIDESSFSRFFDWFIYDFKLIDSGKTIISTFYEE
                     NKKDLSKIEQTIIKQAKESICSFFILEDIIEDNKCIISDIFTEEKFTIVDKSTAEKAK
                     LYSILGARILQSDNTLLFSDIITLYPIAFKSLILDFYKKEYREYENSCDGKPSIKKFL
                     KDWGYLIFQYLENISKNPRFLNSLGQEFIFTISEYKVINHKAAIELLDDTPNITPIIK
                     KTDDLKVYLIASENNPETEGVIELDKNYLNLECYSNSSQSKMKELFDSKFYGVLKHEK
                     DTIKDLNSLINVNKNKKKYKVDRLPPGVKNKKEMDSILDDYYVQWLDEPLDILGGKSP
                     KEASLSEDGKLQLHTILTELEDIYEIARNRGEPYYNVNILRTKLNL"
BASE COUNT         2056 a          907 c         1143 g         2124 t
ORIGIN      
        1 gatgtgccgg ttgcaaggtc tagtaataac tgagcccctt taaattcctt tattaactta
       61 gttttccatt tcttatgaaa ccccatgctg aaaaatgtat tcagcgggtc gtataccttt
      121 gctatatttt caaataaatc tttggtctcg ggcatggttt tgtattaata aatctctaga
      181 ctatatcagg aaaaacggaa aaaaatattt atatggtatt aaatgtctaa ctgactaatt
      241 atttctttaa tatgctctga atggttaagt acataaaaat gtatccctgg cacaccactg
      301 ctcagaaggt cattagcctg tattatgcac tgatctatac cgatcttttt tgccttggta
      361 tcatcactgt ttgcctgctg cagatcatct tttagatacg caggaatctt tgagccgcac
      421 attgaggata ttctctgaat ttgttttgta gatagaattg gcattagacc tggtataaca
      481 ggaagatcga ttccatgtga ttttgttata tctataaatt catagtaata ctgatttaaa
      541 aagaaaagct gagttataat aatatcagag ccagcattta ttttcttttt gaggttttct
      601 atgtcatctt caatggattg agcttcaagg tgcttttcag gataccctgc aacagcaata
      661 ccaaacttta agttgtttct ccgctcataa tcccttatgt gtgcaacaag ctcattagca
      721 tatctgtagc tgttttcatt aacgacacca tcctttggag gatcacccct gagagcgact
      781 atgttgtgga tattatttcc agcaatatta tcaagaattt gatcaatatc atctcttgtt
      841 gacccaacac aggtaaggtg gcaggcagtt tcagttgaca gctcattttt aataaatgat
      901 gcaatatcaa gggttttatc acgagtagta cccattgcgc catatgtgac agtgatgtag
      961 cttggattta atccaataag ttcaggcaga gtgacatctt ttagtttttt aagaccttca
     1021 tccgtcctgg gcggaaatat ctcaaatgaa ataactggtc tgtcattaac ttgataatat
     1081 tctttaaata acattttata atgatttata ttatatataa gtaataatta agatattact
     1141 ttaatattta tttttcttca agtatataat aattcaaaaa aatgttttgt aatttattta
     1201 tttaacacta aattaagggg ctaaatatag cataattata ttatattaaa tatattcaat
     1261 catgaaaatt gccttaattc taattgctca attctttttc atatttgttc cggcttattc
     1321 cgatactacc cctaagtcat atcaaaaagt acaaagagct ttttatgtta tgggtacaag
     1381 ccttgagttt aatcttcttt gcaagaatag gacacactgt ttagatgcaa taaatgaatc
     1441 aattgtggaa gttaatagga ttgataaaat atttagtaac tacagggcag acagtgaact
     1501 atcagctatt agaatcaaca aagatcagta cataaaagta tccagagagt tttttgaact
     1561 taccggattt tcttatttta tatctgctct aacaaatggt gctttcgata tatctgtggg
     1621 accactagta gacatctgga aaaaagaagc aaagaaaaat aaaattccta agactagttt
     1681 aatagaatca atacgtgata agtgcatagg gtttgataaa ttaaaattat actctataca
     1741 taaaaagatc ggttttagtt caagttgcat aaaactggac tatggagcag taggtaaggg
     1801 gcatgtatta aaaaaattag cagagctgtt ggtaagtaag aagatagaaa acggactgat
     1861 taattttggt ggaaatattc atgctcttgg taaagattta gacggtaagc cttgggtaat
     1921 aaaaattaaa gaccctgata atagtaacaa atatataaaa actattaatg tgagcaatat
     1981 gtctgtatca acgtccggag gatatgagag atatttttta gtaaaagaca ggaaatattc
     2041 tcatatcata gatccaagaa caggttttcc tgtaagccgc ttctcttcag ttactgtggt
     2101 atcggaaaat ccgctttttg ccgatgctct gtctactgct ttttctgtat taagtattga
     2161 agaatctaaa aaaataatag atcaattaga taagatagca gtgttaataa tagaaaagca
     2221 tgaaaatgaa ataaatattt ataataatga taattttaat aaactagtat taaactagaa
     2281 agttgttagg ttttatagaa ttttaacata ttgtaattcg aggaaacacc catgatagtt
     2341 gaaactagat ttccggatca aatatatatt ttaattattt gtaactgtat tgacaaatga
     2401 ttattcaata tgtatatcct agacctaaca tatctataat aagaaattga ttaactatag
     2461 aagactattt gtgatttata agattttatt aataagttat tgacaacaaa aaatcaatta
     2521 tgtataacat ctacctatga tatccataat agagattaaa aacctgaaaa tattgtaaat
     2581 ataaaataaa gggggaaata tttatatgag gatatatttg gatatacaaa aaggatataa
     2641 atatttattc tttacgattt ttattttgtt gttttctgtt gcaagtagca cggttatgtc
     2701 ggaagagatg gataaggaca gcagaataag ggagcttgag agaaaactcg aagcagtatc
     2761 tcaggaagta aataaattaa aagaacagca gggtgtaagt aataaaataa gcgaaatcga
     2821 agataaatta actgtattag tagaagagat tgaaaatatt aaatcttcct cagtaactgc
     2881 tgagcgtaaa tatgaacaaa aacacggaca tgctgagggg gcatcgcagg tttatcatgt
     2941 aggaaaggga ttttcggttg gtggatatgg cgagcttttt ataagtcaga ttgaagaaga
     3001 tgacgacaat acggtggata ctcagcgtgt aatattatat gtgggtcaca agtttacaga
     3061 taatattata tttaattccg agatagaatt tgaacacgga acaacaagca gcaaccagga
     3121 tggaagatct ggatcggcat ctgttgaatt tgccagtatt gatttcttaa tatctgaaag
     3181 cttcaacata aggggagggc tcttattggc gccatttgga atagtcaatg aacagcatga
     3241 gccaacgaca ttttttggag tattaaggcc cgatatagaa actcagataa tccctactac
     3301 atggagagag agcggagcag gtatatttgg cgagattaat gatgttattc cgggcacact
     3361 gagctacagg gcttatttaa tgaacagctt tgattcaaga ggttttaggg catctaacaa
     3421 caggagtctg agagttaaag gcaacagggc gaggtttaac gatattgcat ttgtttccag
     3481 gctggaatat gatcccattc ccggggttaa attcggcggc tcatggttca ttggacagac
     3541 gggacaaaat gaaagcgtag atgggcaaac aattggaggc cttttccaga tgtatgaagg
     3601 agatgtcgat attaattgga gaggatttga aggcagggcc ttattcgtat atacattcct
     3661 ggatgatgcg gagctcataa acgctcaaaa tgggcttgtt ggcgatgact cagtcggcga
     3721 ggagcagttt ggatggtatg tacaaggagc ttataatata atgtcacttg cagacgttca
     3781 tccgtatttt caatatcttg ctccttttgt aagatatgaa caatatgaca ctcaaaaaga
     3841 tgtaccagct ggttttttaa gaaatccagc caatgataga gatgttttga cgtttggact
     3901 taattataaa ccaataccaa atgtagtggt taaagtagat tatcaggtaa gagatgatga
     3961 ggctgattct tcaaataatc agataaacct tggacttgga tatgtgtttt aagttattat
     4021 caaataaaat aataagagaa gctggtaaat tgatttcagc ttctcttatt attaatatct
     4081 tgttaatttc gcctaattta tatgctaagg tttttatgcc aaaggatgag gcgcttaaag
     4141 ttgcttttcc taatgccgat acaattgaga aaaagtctta ttttttaaag cctgaacaaa
     4201 taaatgatgt tgaagagcta tcaaaaacaa aagtaggttc taagctgttt ttgttatata
     4261 tcggcaaaaa agacggcagg gatgtgggtt atgcagcaat tgacactcac accgtaagaa
     4321 cgaaaacgca aactataatt gtagtgataa atcctgacgg ctctttaaac tatactgaaa
     4381 tcctggcttt ctttgagcct caggaatata agccaacgga gggctggatt gatttattta
     4441 aagaaaggaa attaagtgac ggaataaaaa taggttatga tatacctaat atatcgggag
     4501 ctactctttc ttctaacgct actgcaaatg cggtgaaaaa ggtgctggct atttttgagg
     4561 tagtaatacg aggggcaaat taagattgag atattttatt tcaaacgata tcaataaaaa
     4621 tgatttgtta aagatcatta ttggcttcac tatactgttt tttgtatttc tatggattac
     4681 aaacctgctt ttatatttac agatcgggtt tagctatgaa tcggtggtac aatattatct
     4741 gggctccgaa gaaagcttca ggcctgctaa gagttatata ggccttctac aggaatctca
     4801 ttttcatttc ttctctatgg cgatacttct ggttactcta aaccatttaa tactatttgc
     4861 ttctataaaa aatatatata aattaatttt gattttgtct tcatttttgt ctgcgcttgg
     4921 cgatatttct gcaggatggc ttataaggta tgtaagcccg tatttttctt atctaaagat
     4981 tgtttctgtg atcgtgttac aaatcagttt agcgcttctc attatagtac taataatatt
     5041 tatatataac aaggggaaga aggctctgta tacgggatag agactgctac agatttaact
     5101 tagttctaag aatattgaca ttgtaataag gctcgcccct gttccttgca atttcataaa
     5161 tatcttctaa ttcagttagt attgtatgga gctgtaattt accatcttca gaaagtgatg
     5221 cttctttggg tgatttccca ccaagaatat ctaaaggttc atctagccac tgtacataat
     5281 aatcatccag gatcgagtcc atctcttttt tatttttaac tcctggaggc agtctgtcta
     5341 ctttatattt tttcttattc ttatttacat ttataagcga attgagatct ttgattgtat
     5401 ctttttcatg ttttaataca ccgtaaaatt tggagtcaaa gagttctttc atttttgatt
     5461 gcgagctgtt tgaataacat tcaaggttta aatagttttt atcaagttca ataactccct
     5521 cagtttccgg attattctcc gatgctatta aataaacttt tagatcatca gttttcttta
     5581 taattggagt aatattgggg gtgtcatcga gaagctcaat tgcagctttg tggttaatta
     5641 ctttgtactc tgaaatagtg aatataaatt cttgtcctaa tgaattaaga aatctggggt
     5701 ttttggatat attttccaga tactggaaaa tgaggtaccc ccagtctttt aaaaatttct
     5761 ttatacttgg tttaccatca catgaatttt catattctct atactctttt ttatagaaat
     5821 ccagtattaa cgatttaaaa gcgattgggt agagcgtgat aatgtcagaa aaaagcaggg
     5881 tgttgtcaga ctgaagaatt cttgctccca aaatactata taatttagct ttctcagctg
     5941 tagatttatc tactattgta aatttttctt cggtaaatat atcggagata atacatttat
     6001 tatcttcaat aatatcttct aatataaaaa agctgcagat actctctttt gcttgtttta
     6061 ttattgtctg ttctattttg gataaatctt ttttgttttc ttcatagaag gtagaaataa
     6121 tggttttgcc tgaatctatt aatttgaaat catatataaa ccagtcaaaa aatctggaaa
     6181 aagatgattc atcaatttca tccattgcca aatcttccgg aagtaaatta
//
LOCUS       DBNGSV010000130         5462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_371017, whole genome shotgun sequence.
ACCESSION   DBNGSV010000130 DBNGSV010000000
VERSION     DBNGSV010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_371017"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..455
                     /locus_tag="AC159U_04490"
     CDS             129..455
                     /locus_tag="AC159U_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336349.1"
                     /translation="MRLFSILAVFVLAGLFLVALTDNTVAGIPGQGICKIDCQGIAEM
                     EADAFVDTDCEGLRGEDRKECQIFRNIIVKCFYVECLEEECGMDEVPEESSICEVLDE
                     LCLDQE"
     gene            632..844
                     /locus_tag="AC159U_04495"
     CDS             632..844
                     /locus_tag="AC159U_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336350.1"
                     /translation="MKYIYDFNKTDSSLSYEEISRSVRGCKVRTMALSKGTFELVRSG
                     VEVDKDLMKFTNPPKLSNQHVSKFMY"
     gene            993..1394
                     /locus_tag="AC159U_04500"
     CDS             993..1394
                     /locus_tag="AC159U_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3336351.1"
                     /translation="MDILGTTVITKVSSAETGGAYYVFHLLIPPSGGIPAHVHENEDE
                     IFCIIEGDLEITVDGSVVNCTKGDVQNLPRGIVHGIKNTSNSESKSIVFITPGSSFED
                     FFSKLTLLSKGPDTDMGEVIKLSAEYGIKFV"
     gene            1437..1754
                     /locus_tag="AC159U_04505"
     CDS             1437..1754
                     /locus_tag="AC159U_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3336352.1"
                     /translation="MLIIDHDKDVKFNKDHAIAEPLFVNENNRLLRFALLPGQEIKEH
                     NSPSSPVFIKIIRGRGVFWGEDKKEQELDKDSVIVFKKGEMHGIKALDEELVFIAVLH
                     GAP"
     gene            2054..2287
                     /locus_tag="AC159U_04510"
     CDS             2054..2287
                     /locus_tag="AC159U_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336353.1"
                     /translation="MKKTLNDLTVDEQRYNSSSPLRTGIYVSAIVPDADKRESVDISV
                     LDKESMNKWLRTKSREYLEELVRYFSGHEVSRD"
     gene            2758..3588
                     /locus_tag="AC159U_04515"
     CDS             2758..3588
                     /locus_tag="AC159U_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HIR3336354.1"
                     /translation="MNKITISKTLGFFAVFILALFLYSMPSHAGTISFFEIRDDANGG
                     VCATADSGAHSWDADSKTCTLGQDISLHVTFSDNGLTLDCNGNTIQGTGSNTGIIINN
                     DKNIVVKRCTIDDWNTGISASNRSSNLLIFKNEFTSNFIALNFNGVSNSKVIDNHADN
                     TNTDSGFRFFATKTTEIINNRSEALDTGFSLLEGSVSNRLIANSSTGAFVGFVLTFGS
                     ARNEFIYNTATSHTGGVGFGFNLFESGREIYSNTIFPMEISLVLELMTIPIITLLKIT
                     "
     gene            3546..3731
                     /locus_tag="AC159U_04520"
     CDS             3546..3731
                     /locus_tag="AC159U_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336355.1"
                     /translation="MDDNSDNNTFEDNVADNNANDGFFQHATSSNTYIDNDCNDNSTA
                     NSNPPGLCNSVDPTPPP"
     gene            3772..4812
                     /locus_tag="AC159U_04525"
     CDS             3772..4812
                     /locus_tag="AC159U_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HIR3336356.1"
                     /translation="MKTIKLITLLLFASLMFTALSQHAQAGPPPAFVGYTISDDATGG
                     DCNTIGVWDSSTKTCVLTQDVECITNATTDICMEIQSDDIVFNCKEHQLFDNFGGPSG
                     TGVRILGHSGVKIKRCDFDNFDIGIQVLNSDNINILLNTFTFTDLQSINLQSTDSSYI
                     VGNEALDGSTDQMLFHNITKNNHVIGNKINAGSTFAGFVISDCTNDSFIANSSNDAQD
                     SVDGDGFNINGCVRSEFINNSVDGNDGSGFVYQNSSRSTIFANIADGNKLESMTIGGG
                     FVIDNNSGSLTFEQNISDNAQPGSVDGIGIIDNSTGKKTSGTANTYEGNTCDGNAGGS
                     SSPSGLCTGLNP"
     gene            complement(4878..5321)
                     /locus_tag="AC159U_04530"
     CDS             complement(4878..5321)
                     /locus_tag="AC159U_04530"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003727 - single-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR3336357.1"
                     /translation="MQLCPFREGPAILQTLEELNHKPDLMVFDGQGIAHPRGLGIASF
                     IGVLTGIPSFGIAKKKLFGDYTEPDVKAGSVSNLYHPEDKSIIGSVVRTKTKVKPVFV
                     SVGHKISLDKSVELALEFTRGYRLPEPTRQAHLFVNEVRREMLGK"
BASE COUNT         1774 a          972 c         1100 g         1616 t
ORIGIN      
        1 aaaattctaa cttataatct ggtactatta tatggggcaa ggtcagtata cccctcctaa
       61 caaaaaccgc ttgcttaaga ttttacaaag taatataatc aaactaataa acgaaggagg
      121 tgatggatat gagattattt tcaattttag cagtatttgt tttagcaggg ctgtttctgg
      181 tggcacttac cgataacacg gttgcaggaa tacccggaca aggtatttgt aaaatagatt
      241 gtcagggcat tgctgaaatg gaagccgatg cttttgttga tacggattgt gaaggactca
      301 ggggagaaga cagaaaagaa tgtcagatat ttagaaacat aattgttaaa tgcttttatg
      361 tcgagtgcct cgaagaagag tgcggtatgg atgaggtacc agaagagtct tcaatttgtg
      421 aagtattgga tgagctctgc ctcgaccaag aataagattt ttaattaaag tattgttaat
      481 tcaagggaag cagaacgtgc cgcttccctt tttttgtgcc ctttttaacc tttaattacc
      541 gcaataaaaa acagattgta atgatgccct tgtaaaggct tatttgatgc ttattgtaag
      601 tctataccaa ttttacaaag gggctgcaaa gatgaaatat atttatgatt tcaataaaac
      661 agattcttca ctttcatatg aggagattag ccggtcggtc aggggctgca aggtgcgcac
      721 tatggctcta tcaaagggca ctttcgagct tgtacgaagc ggtgttgaag tagacaagga
      781 tcttatgaaa tttacaaacc ctccaaaact tagtaatcag catgtttcaa aatttatgta
      841 ctgaccaagt gagcccttgt atctaacaag tccacagcta agcctggcac cgcttgcctt
      901 tttttgtacc caaaatatta ctgattaatt tacaatacat ttgatgttat catttgtata
      961 atgaaatcag atcactataa gagggaagag aaatggatat tctgggtaca accgttataa
     1021 ccaaagtaag cagcgcagag actggcgggg cgtattatgt gtttcactta ttaataccac
     1081 caagcggggg aatacccgcg catgtgcatg aaaatgagga tgagatattc tgtataattg
     1141 aaggtgatct tgaaattaca gttgatggca gcgtagtaaa ctgcactaaa ggtgatgttc
     1201 aaaatctccc aagaggtatt gtgcacggga taaaaaatac cagcaattcg gaatctaaat
     1261 cgatagtctt cataaccccg ggaagcagtt ttgaggattt cttttcaaaa ctcactctct
     1321 tgtccaaagg gcccgatacg gatatgggtg aagtaataaa gctttctgcc gaatatggga
     1381 taaagtttgt gtagaataaa tagctttaac tctgcttgtg ttatataata aaaagtatgt
     1441 taataatcga ccatgataag gatgttaagt ttaacaaaga ccatgcgatt gccgagccgc
     1501 tctttgtaaa cgagaacaac aggctgctca ggtttgcact tcttcccggt caggaaataa
     1561 aagagcacaa ctcaccgtct tcgccagtgt ttattaaaat aataaggggc aggggtgttt
     1621 tttggggaga ggacaaaaag gagcaagagc ttgataagga ctcagtaata gtctttaaaa
     1681 agggcgagat gcacggcata aaggcacttg atgaagagct ggttttcata gcagtgctgc
     1741 atggcgctcc ttaatactca aagattgcag aaacaaaatg gagcccgttc ctatccagaa
     1801 tatttagcat tctattcact attctaatca attccagaaa agcatgacag gtgtatttat
     1861 tggattgatt tatagtctag aaggtgaagt gatagaaata gcggagtgct gtttagaatt
     1921 ataactgcca gggaataaac tcataagata aataatgttt gatacaattt agcgttacta
     1981 tttgtcactg gctggctggt ttgatatagt aaattaacca ttacatagac ttgttataaa
     2041 ggaggcacat gatatgaaaa aaactctaaa cgatttgact gtagacgagc aacgttataa
     2101 ttcatcatct ccgcttcgca ccggcatata tgtaagcgct attgtgcccg atgccgacaa
     2161 aagagagtct gtagatatat cagttctcga caaggaatct atgaacaagt ggctgcgcac
     2221 aaaatcaaga gaatatcttg aagaactcgt ccgatatttt tcaggacacg aagtttcaag
     2281 agattgataa gcggcttata aatttaaatc tgaaaggaac aggattcact gctattcttt
     2341 tattatagat aaattgtatg atacaaaaaa ccccgctttc agacaattct gagagcgggg
     2401 acagtatgta ttcttgattt attatttgtc agtcacagtc aacattggaa aattctgttc
     2461 cattacctgc ccctgtgtac ttcttgcact gagtagaggc atcacctatt aagaaaaccc
     2521 tgaaatcgtt attatccgta taattatact ttaccttgtt tcctgttccg tttacttcga
     2581 atcagtcatt cgtttttgtt aattgctgca atttaagcac tcattaatat ttaactcaag
     2641 tattttgtta atgcttaagt atggatatat cttttgatag aaccaaagta ccaagaaata
     2701 attgacatca atcttctaat ttactacaat catttctatt aagggaggtt caccgctttg
     2761 aacaaaataa caatatctaa aacactcgga ttttttgcag tattcattct cgctttattt
     2821 ctttattcaa tgccgtctca tgcaggtacc atatcatttt ttgaaattag agatgatgct
     2881 aatggtggtg tttgtgcaac ggcagactca ggcgcccaca gctgggatgc tgactcaaaa
     2941 acatgcacgc tcggtcagga cataagcctt cacgttacat tcagcgataa tgggctcaca
     3001 cttgactgca atggaaatac gattcaaggc acaggtagta atacaggtat tattattaac
     3061 aacgataaaa acatagtagt gaaaagatgt accattgatg attggaatac tggtattagt
     3121 gcttcaaaca gatcatcaaa cctgcttata tttaaaaacg aatttacaag caatttcatt
     3181 gctctgaatt tcaatggtgt atctaactct aaagtaattg ataatcatgc ggacaataca
     3241 aatacagata gcgggtttag attttttgcc accaaaacca ctgagataat aaataataga
     3301 tcggaggcac ttgatactgg ttttagctta ctagaaggct ctgtaagcaa tagactcata
     3361 gcaaattcaa gcacaggagc tttcgtgggt tttgtactta cttttggcag tgcaagaaac
     3421 gagttcatat ataatactgc aacatcccat acgggcggag taggatttgg atttaattta
     3481 tttgaatcag gaagggaaat atattcaaat acaatatttc caatggaaat aagtttggtt
     3541 ttagaattga tgacaattcc gataataaca cttttgaaga taacgtagct gacaacaacg
     3601 cgaatgacgg tttttttcag catgcaactt cttccaacac atacatagat aacgactgta
     3661 acgacaattc aacagccaat tccaatcccc cgggtctttg caactcagta gatccaacac
     3721 ccccaccata ggacttgtga ggcaaaaata taattaaaaa ggagaaataa tatgaaaaca
     3781 ataaaactaa ttacactttt actttttgct tcattgatgt ttacggctct ttctcagcat
     3841 gcacaggcag ggccaccacc ggcatttgta ggctatacaa tatcggatga tgccacaggt
     3901 ggagactgca atacaatagg tgtatgggat agctctacta aaacgtgcgt tttaactcag
     3961 gacgtggaat gcataactaa tgctactact gatatatgca tggagataca aagtgatgat
     4021 atagtgttta actgcaagga gcatcagctc tttgataatt tcggtggtcc ttcaggtact
     4081 ggcgtaagaa ttttgggtca ttcaggcgtt aagataaaga gatgtgattt tgataatttc
     4141 gatattggca tacaggtgct caattcagac aatatcaata ttctattaaa tacatttact
     4201 ttcactgatt tgcagagtat taatcttcaa agtacggaca gtagttatat agtcggaaat
     4261 gaagcactcg acggttctac agatcaaatg cttttccata atattacaaa gaataatcat
     4321 gtaataggaa acaaaataaa tgccggctca acttttgcag gatttgtcat tagtgactgc
     4381 actaatgatt cattcattgc aaactcatct aacgatgctc aagactccgt ggacggtgat
     4441 ggattcaaca ttaatgggtg cgtgaggagc gaatttataa ataacagcgt cgatggaaac
     4501 gatgggtcag gttttgtcta tcaaaattct agcagatcta caatatttgc aaatattgca
     4561 gatggtaaca aacttgagag tatgacaatc ggaggtggat ttgtaattga caataattca
     4621 ggatctctca cttttgagca gaatatatct gacaatgcgc aacctggctc tgtcgatggt
     4681 atagggataa ttgataatag tacgggcaaa aaaacctcag gaacagctaa tacctacgag
     4741 ggaaatacat gtgacggaaa tgcagggggc agttcatctc catcgggcct ttgcacgggc
     4801 cttaatcctt aaacattata atcaaataga attaattaat tctaaagccc gcccagtgct
     4861 gcactcggca ggctttttta tttgcccagc atctcccttc tcacttcatt tacaaacaga
     4921 tgcgcctgac gggtaggttc gggtagtcga taaccccggg tgaattctag ggcaagctca
     4981 accgatttat ctaaacttat tttatgacca actgatacaa aaacgggttt gaccttagtc
     5041 tttgtcctaa caactgaacc aattatggat ttatcttcag ggtgatataa atttgataca
     5101 ctgccggcct taacatcggg ttcagtataa tctccaaaga gtttcttttt tgctatacca
     5161 aatgagggta ttcccgtaag aacccctata aaacttgcaa tgccaagacc gcgtgggtgg
     5221 gctatgccct gaccgtcaaa taccatcaaa tctggcttgt gatttaattc ttccaaagtc
     5281 tgtaatatag cagggccttc tctgaatggg cataattgca aaaaatgtgt gctgcaaatg
     5341 gggttttagc ctttatttac tatattataa tgagaaaaaa gttaaacagg tttaactatg
     5401 aacaaaaaac tgtccccttt gcagtgcaca aaaagcagtc aagaaaaacg gaaaatactc
     5461 aa
//
LOCUS       DBNGSV010000131        10933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1064279, whole genome shotgun sequence.
ACCESSION   DBNGSV010000131 DBNGSV010000000
VERSION     DBNGSV010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1064279"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="AC159U_04535"
     CDS             complement(<1..1619)
                     /locus_tag="AC159U_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR3336358.1"
                     /translation="MRVYELSKELGVVNKELIEAAKSLGISLKSHASSIRDDEVIKIR
                     QVLTKSKGTDTSKVDNNKEQPVSKEKVKVFKSDSGQEVVERRKGTRVILRRKKKVEEE
                     VEPEVVEPVDEVSETAAEIPDSTLTEQEQIIEAGSELQEISIDIDKTKTEQEPSSPDA
                     QDSLQAVTQQTEAKDVKENIKSKEIEQSESTDIPTEGEAQKETKKVKKKGKVVKPRKE
                     EIIDEDSLEELRKAFRTKLPVRKKEYLVENRRPKAKFHHSAKFNKGHDRHVTASQSTS
                     ATIAEPQSNVQVIAEPAKPATKVIKIQESINVAELAKKMGVKVGSVLRKLLSLGVTSS
                     INQSIDHETASILAQEYDYEIVLDIFEEDDHLVEQSKDEQENLVSRYPVVTVMGHVDH
                     GKTTLLDTIRKTKVVEGEAGGITQHIGAYSVPVQDKKVTFIDTPGHEAFTAMRARGAE
                     VTDVVILVVAADDGVMPQTVEAVNHAKAAEVPIIVAVNKIDKPEAEPDRVKRELSEIG
                     LVPEDWGGDALFTEVSAKTGKGIDELLELILL"
     gene            complement(1725..3095)
                     /gene="nusA"
                     /locus_tag="AC159U_04540"
     CDS             complement(1725..3095)
                     /gene="nusA"
                     /locus_tag="AC159U_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3336359.1"
                     /translation="MVVTELTRILDSVSKDKGVNREVIVNAIEEAVQLAAERLFRSEG
                     KFTELEVHFNEEDGEVELFEFKKVVEEVEDPDVEIAIKEAVNLDPEAELEDFIGVKIS
                     PEYTRIAIQNAKQKILQKLKEAEGKVIYEEFKTRKGELVGGIVRRIERRTAIVDLGKT
                     EAILPPEHQVPKEYYKQKERVRAILIEIQESKKGPKLILSRAHPDFVKSLFEAEVPEI
                     SEGVMEIKAISRDAGSRTKIAVHSNDPDVDPIGACVGMRGARVQNIIHELRGEKIDII
                     PWSKDSARFVCNALSPARINKVIIDENHNAMEVVVDDDQQSLAIGRRGQNVRLASQLT
                     GWKIDIKTETQLNKELQDVMTVLISLPNISDVTAKLLYNEGYHTLEDIAFSDPDLIAK
                     AGNLKTAEEVESIQKAARIALKDKLENISIDDEVVVDTNSADNIAETENKPESDSEPE
                     TNPNPE"
     gene            complement(3109..3579)
                     /locus_tag="AC159U_04545"
     CDS             complement(3109..3579)
                     /locus_tag="AC159U_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR3336360.1"
                     /translation="MVLEKNEILKKIEELISPVLRDKQLELIDIEYKPEGRGKVLRVY
                     IDKQGGVNIDDCTAVSRELSVIMDIHDIINGTYNLEVSSPGLTRVLKRNEDFIRNIGK
                     KLKLKLIEPLNKQYVLRNAVLQSFENNTLNINYEGAEYEIAIDNVSKANLELDI"
     gene            3725..4243
                     /locus_tag="AC159U_04550"
     CDS             3725..4243
                     /locus_tag="AC159U_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336361.1"
                     /translation="MKINKLNNLNILNTIKRSIHFDKNLELEIEDKEEYNNQAILITV
                     LASLFAAIGIEGTNIFGVIISTIIELIACAFWIAVIVVMVFKILEVRIIPIVLARLIG
                     ISLYPLMLMLFAFVPYIGTHIAIIGIILAIITVSLTIRSHTELEPGLSLALSMIGSIP
                     FIVLNFYLSYQS"
     gene            complement(4248..6320)
                     /locus_tag="AC159U_04555"
     CDS             complement(4248..6320)
                     /locus_tag="AC159U_04555"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR3336362.1"
                     /translation="MTISFALILFTHIIAFLGFFTLSISGQVSPLTMGVFYVSLVLSF
                     INDHYKKNYQFNQTVSTVIAFVVVLYILASIVYFGQEVFQGILNFLIYLQVVRLLSAK
                     GMREIIQIFLLSFFQFMAGAILSIEISYGFALIVYVFTALWGLIIYNLKKESDDAGVK
                     SSETVIGRSFMGSSLALSICVISISVVLFLMLPRLKTEYFSSSFINPKKLKTGYSDTV
                     KLGKVGEIKKDHSAVMRVTMLEGRGKLRDDLYWRGIALDHFDGVTWSVDSEKFGDYEK
                     RHSTNRYGIFSVKNNAENVIAHEIITEPIDTDILFAINEPVGYSVSGGSKSRYGASIY
                     EVNNSYFLPFNPRQRIKYRAYSSLKNYPDEKLGDESDQYSFYIKQRYLQLPEVSSDIE
                     NMALDITKNENTVYGKSKAVLRYLLEEMNYTLTLKSGTKEFPLETFLLEKKEGHCEYF
                     ATSMVVLLRMNGIPSRIVNGFIGGTWNKHGNFYVVKESDAHTWVEVYFPKRGWITFDP
                     TPSSDSTLGGGSLTAISSYIEYLKYRWQRYVIDFSQGDQVRLFSSARQKISWNKNKAL
                     NNVNLKSSKNRKFVIIIAAALLIIFILYNSNRFLGGRKNKKNKQLAVTKIYNKSVSYL
                     SKKGFSKKDSQTANEFAKYVLATGGSKYEIVDEFTKKYSLMRYKNNYSDSDISSLKQL
                     LSLIKKVK"
     gene            complement(6317..8326)
                     /gene="uvrB"
                     /locus_tag="AC159U_04560"
     CDS             complement(6317..8326)
                     /gene="uvrB"
                     /locus_tag="AC159U_04560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3336363.1"
                     /translation="MEKLFNLTTEYKPQGDQPGAIEKLTSGLASNKKHQVLLGVTGSG
                     KTFTIANVINNVQRPTLIIAHNKTLAAQLYGELMDLFPDNSVHYFVSYYDYYQPEAYI
                     PTTDTYIDKDVAINETIDRLRHAATSSLFERRDIIIVSSVSCIYGIGSPNDYYGLLTT
                     IETGGRLDPKILLNKLKDVQYQRTNNDLTRGTFRYRGDTVEVFPSHQENTAIRVEFFD
                     GVIESIKEIDPLRGKTLKKVDKTIIYPSSHYVASQDKIKIAIEAIKEELKQRIKILEK
                     ENKYLEARRIEERTNYDLELLTTMGFCNGIENYSRHITGRSEGEPPWTLIDYFPEDFL
                     VVIDESHQTMPQIRGMYEGDRSRKKTLVEYGFRLPSALDNRPLNFKEFESKVRSAIYV
                     SATPANYEITKSNGAVVEQIIRPTGLIDPEIEIKPAENQVDDLLQEIKSRIDIGDRVL
                     ITTLTKKMAEDLSEYYKDLGLRVNYLHSDIDALERIKIIRSLRLGEFDILIGINLLRE
                     GLDIPEVSLVVILDADKEGYLRSETSLIQVFGRAARNVRGKVILYADKMTGSMQRAIS
                     ETYRRREIQEKFNTANNITPKSIEKNISDILSSIFEYDYFTVPKDIGVELDNVAPNKI
                     PSMVKLLTKDMKKASKKLQYEKAAEYRDKITKLRELELEYAEQLK"
     gene            complement(8382..8792)
                     /locus_tag="AC159U_04565"
     CDS             complement(8382..8792)
                     /locus_tag="AC159U_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3336364.1"
                     /translation="MRIYRTLYLILTILMISQIPAFAGAKYKWWKNSDVVSNLQLSPA
                     QVSTIEQIFNLHKTKLHETKQFIIQKQKELNQKLKDPQSDKQEISNLAEQVDVLKLQL
                     GKIHRNMQLEIREVLTPDQRKKLHEMWSGRKHKK"
     gene            complement(8797..9285)
                     /locus_tag="AC159U_04570"
     CDS             complement(8797..9285)
                     /locus_tag="AC159U_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336365.1"
                     /translation="MKCDEIKGMYDDFKLQVLSKEESVAIDEHLINCNGCKDYFAKNN
                     KLTRLMDTWDEIEPQSNYVSKFWSRMEEKEKNENKGIIGFIKGIRTTFLVPSLAVALF
                     CSLFIVNMYRTSTVNPTYSEQDLQDNRLLQEINDIISVEGSESLQIYGLWNNLEEESK
                     EG"
     gene            complement(9275..9853)
                     /locus_tag="AC159U_04575"
     CDS             complement(9275..9853)
                     /locus_tag="AC159U_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3336366.1"
                     /translation="MESDIDLILRVQSGDESAFSEILKRYYSKILNYVYRYTNNRETS
                     EDLTQEIFMRVHRSVKNYRPEAKFSTWLYKIATNLCLTEVSTRSRKHASSLDEIQENV
                     GSLEDIKSVDQADVRYRSEIKEIIFEAMNTLSENERSAIMLCKYEQLSYEEVAQTLDC
                     TVGAVKTYVYRGRMKLIEKLKPYLDEVEMDEM"
     gene            10009..>10933
                     /gene="cobA"
                     /locus_tag="AC159U_04580"
     CDS             10009..>10933
                     /gene="cobA"
                     /locus_tag="AC159U_04580"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3336367.1"
                     /translation="MQKQGKVYLIGAGPGDPGLITINAVKCLNSADVIVYDYLVNTKL
                     LDHRKHNSEVIYVGKKSGHKELSQEKINKLLIKHAKNGKTVARLKGGDPFIFGRGAEE
                     AIELNNNNIDFDIIPGVSSFYAVPEYAGIPPTHRNVSSTIAVITGHEDPSKNRSNIPW
                     ASLSKISTVIFLMGIRNLSEIVKKLLKNSKSPNTPIAIITWGTYPNQKTITGNLENII
                     KKSQEAQIESPGIIIVGKVVKYREQIQWFEKRPLYGKKILTTRPSSQSKFFVEKLNNL
                     GAETIEFPTIEISAPSSWNKLDKSLKQLKTTD"
BASE COUNT         3146 a         2244 c         1629 g         3914 t
ORIGIN      
        1 agcaatatca attccaacag ttcatcaatt cctttccctg ttttagcgga gacttctgta
       61 aaaagtgcat caccgcccca gtcttctggg acaagtccta tctctgagag ctcacgtttt
      121 actctatcag gttctgcttc tggtttatct attttattaa ctgctacaat tataggtact
      181 tcggcagcct ttgcatgatt aactgcttct accgtctggg gcattactcc atcatctgct
      241 gctactacaa gtatgaccac atcagtaacc tctgcccctc tggctctcat cgcagtaaaa
      301 gcttcgtgtc ctggagtatc aataaatgta acttttttat cctgtaccgg aacactgtat
      361 gcacctatat gctgtgtaat gcctcctgct tctccttcaa caactttggt tttacgaata
      421 gtgtccagaa gtgttgtctt accatggtct acatggccca taacagttac tacagggtat
      481 cttgaaacca aattttcctg ttcatcctta ctttgctcta caagatgatc atcttcttca
      541 aaaatatcca gcacaatttc ataatcgtat tcctgagcca gtatgctggc agtttcgtga
      601 tctatagact gattaattga tgacgtcaca cctaaagaaa gaagtttcct gagtacagaa
      661 cccaccttaa ctcccatctt tttagcaagt tcggcaacgt taatagattc ctgtatttta
      721 attactttag ttgctggttt ggcaggttca gctataacct ggacattcga ttgtggctca
      781 gctatagtgg cagatgtgga ttgggaagca gtaacatgtc tgtcatgccc cttattgaat
      841 tttgctgaat gatgaaactt agctttaggc cttctgtttt ctactaagta ttcttttttt
      901 cttacaggca gttttgttct aaaagctttt ctcaattcct ccaggctgtc ttcatctata
      961 atctcttctt ttcttggctt aacaacttta cctttctttt taaccttctt tgtttctttt
     1021 tgagcctcgc cttctgtagg tatgtcagtt gattcagatt gctcaatctc tttagattta
     1081 atattttctt taacatcttt tgcttctgtt tgttgagtaa ctgcttgcag actatcctgc
     1141 gcatcagggg agcttggctc ttgctcagtc tttgttttat ctatatctat acttatttct
     1201 tgtagttcag aaccagcctc tattatctgt tcttgttctg tcagagtaga atcaggtatt
     1261 tcagctgcag tttctgatac ttcatctaca ggctcaacca cctcgggttc tacttcttct
     1321 tcgacttttt tctttcttct aagaataacc ctggtgcctt ttcttctctc tacaacttcc
     1381 tgtcctgagt ctgatttaaa tactttaact ttttccttag aaactggttg ctccttgtta
     1441 ttatcaactt tagatgtatc tgtgccctta gactttgtta gtacctgtct tattttaata
     1501 acttcatcat cccttataga acttgcgtga ctcttaagac ttataccaag agattttgca
     1561 gcttctataa gctctttgtt tactactcct agttctttag ataattcgta gactctcata
     1621 tgttatattt atagtacttt tcttaaaaaa aatactttag taaatcaata atactacctc
     1681 atttaggttt ataagcaaaa gtgatatgcg agtaatccta caacctattc tggatttggg
     1741 tttgtctctg gttctgaatc tgattctggt ttattttcag tttcagctat attatccgca
     1801 ctattggtat ccacgacgac ttcatcatca atgctaatat tttcaagctt atcttttaac
     1861 gcaattcttg cggctttttg tatgctttcc acttcttcag cagtttttaa attgcctgct
     1921 ttagctatta aatctggatc agaaaaagca atatcttcca gcgtatggta accctcgtta
     1981 tataacagct ttgcagtaac atcacttata ttaggaagag atattagtac tgtcattaca
     2041 tcctgcaact ctttattgag ttgagtctcc gttttaatat ctatcttcca ccctgtaagc
     2101 tgagatgcaa gccttacatt ctgccccctt cttcctattg caagagactg ctgatcatca
     2161 tctaccacta cttccattgc attgtgattt tcgtctatta taaccttatt tatacgcgcc
     2221 ggggaaagag cattacatac aaacctggca gaatccttag accatggaat tatgtcaatc
     2281 ttttcgcccc ttagttcgtg aataatattt tgtaccctag cacctctcat tcccacacaa
     2341 gcgcctattg gatcaacatc aggatcgttt gaatgtacgg caatttttgt tctgctccct
     2401 gcatcacggg aaatcgcctt gatttccatt actccttcgg atatctcagg aacttcagct
     2461 tcaaataaac tttttacaaa atccgggtgt gctctggata gaataagctt tggtcctttc
     2521 ttgctttcct gtatctctat taatattgcc ctgactcttt ctttttgttt gtaatattcc
     2581 tttgggacct gatgctctgg cggaagaatg gcttctgttt taccaagatc aacaattgca
     2641 gtccttcttt cgattctcct tacaatgcct cctacaagct ccccttttct ggttttaaat
     2701 tcttcatata taactttacc ttcagcttct tttagctttt gtaagatctt ttgctttgca
     2761 ttctgaatag caattctggt atattccgga cttattttta caccaataaa atcttcgagt
     2821 tcggcctcag ggtctaaatt tacggcttct ttaatagcaa tctccacatc tggatcttca
     2881 acttcttcta caaccttttt aaactcgaat agctcaactt caccatcttc ttcattaaag
     2941 tgcacttcga gctcagtaaa tttgccctct gaacgaaaca gcctctctgc agcaagctga
     3001 accgcttcct ctattgcgtt tacaataact tctctattta cgcctttatc tttactaact
     3061 gaatctaata ttcgtgtaag ttcggtaaca accatctgtc actacctcct aaatatcgag
     3121 ctccaagttt gcctttgata cattatcaat tgcaatttca tactctgcgc cctcataatt
     3181 aatattcaga gtattattct caaaactctg taaaactgca tttcttaaaa catattgctt
     3241 atttaagggt tctataagct ttaattttag ttttttacct atatttctta taaaatcttc
     3301 atttcttttt aaaactcttg taagacccgg tgaagatact tcgaggttat atgtaccatt
     3361 tattatgtca tgaatatcca tgataacgct caattctctg ctcacagccg tacagtcgtc
     3421 tatgtttact ccaccttgtt tatcaatata cactcttaac actttccccc ttccctcagg
     3481 tttatactct atatcaataa gctctaattg cttatccctg aggacaggac taattaattc
     3541 ttcaattttt ttcagtattt catttttttc caagaccatt tataatagcc tttaatattt
     3601 tcttttatac caaataaaaa agcgggtaaa aacccgcttt tgatcttaga tattacccaa
     3661 tgctatatat tgagcaagta tgaaggaaag tatattaatt acaataaaat attattttca
     3721 tattatgaag ataaataaat taaacaatct gaatatctta aacacaatta agaggtcaat
     3781 ccactttgat aaaaatcttg aattagagat tgaagataag gaagaatata acaatcaagc
     3841 aatactaatt acagttctag catctttatt tgcagctata ggaattgaag gaaccaatat
     3901 ctttggagtt atcatttcaa ctattataga gcttatagcc tgtgcattct ggattgccgt
     3961 tatagttgta atggtattta aaatactaga agtcagaata attccgattg ttctagcaag
     4021 acttatagga atatcattat atccacttat gctcatgtta tttgcgtttg taccatatat
     4081 aggaacacat atagcaataa ttggtataat attagccata ataacagtat ctctaactat
     4141 aagaagccac actgagctgg aaccgggact ttcactggct ctttccatga taggctctat
     4201 accatttatt gttcttaact tttacctttc ataccaaagc taataaatca tttaactttt
     4261 tttattaacg ataaaagttg ttttaaagaa gagatgtcgc tgtctgaata attattcttg
     4321 tatctcatta atgagtattt ttttgtaaac tcatcaacta tttcatattt ggagccaccg
     4381 gttgctaaga catacttagc gaattcattt gctgtctgac tgtctttttt actaaaacct
     4441 ttttttgata agtatgaaac acttttattg tatatttttg taacggcaag ttgtttattc
     4501 ttcttattct tcctgcctcc caaaaacctg ttgctgttat atagtataaa aatgattaga
     4561 agagctgccg cgataataat aacaaatttt ctattttttg aactttttaa attgacatta
     4621 tttagcgcct tatttttatt ccaggaaatt ttttgccttg cacttgaaaa cagcctgacc
     4681 tgatcaccct gactaaaatc tattacatat ctctgccagc gatattttaa atactcaata
     4741 taagaagata ttgcagttaa gctccctcca cccagagtag aatcagaaga aggagtagga
     4801 tcaaatgtaa tccatcctct tttgggaaaa tatacttcta cccatgtatg tgcatcactt
     4861 tcctttacca cataaaaatt gccatgctta ttccatgttc caccaataaa accatttacg
     4921 atacgtgatg gtattccatt catcctaagc agtacaacca ttgatgttgc aaaatattcg
     4981 cagtgacctt cttttttctc taacaagaat gtttccagag gaaactcttt tgtaccactt
     5041 ttaagagtaa gtgtatagtt catttcttca agcagatatc tgagcactgc tttggattta
     5101 ccgtataccg tattttcatt ctttgttata tctaaagcca tattctcaat atcactgcta
     5161 acttctggca gctgcagata tctttgttta atataaaaag aatattggtc tgattcatct
     5221 ccgagttttt catcggggta atttttgaga cttgagtacg ccctgtattt tatcctctgt
     5281 ctgggattaa agggtaagaa gtaagaattg ttaacttcat atatactagc tccatatctg
     5341 gatttgctcc cgcctgaaac tgaataacca acaggttcat taatcgcaaa aagtatatcc
     5401 gtatctatag gttctgtaat aatttcatgt gctataacat tttcagcatt gttcttaaca
     5461 ctaaatatcc catatctgtt tgtactgtgt ctcttctcat agtctccaaa tttctcactg
     5521 tccacactcc aggtaacccc gtcaaaatga tccagcgcta tccctctcca gtacaggtca
     5581 tccctaagtt tgcctctacc ctcaagcatt gttaccctca ttacggctga atggtctttt
     5641 tttatttccc caaccttacc aagcttcaca gtatcggagt atcctgtttt tagctttttc
     5701 ggattaataa aagagcttga aaaatattcg gtttttaatc ttggaagcat cagaaaaagt
     5761 actactgaaa tagatattac acatatggat agcgcaaggg aggatcccat aaacgatctt
     5821 ccaattacag tttcactgga ttttactcct gcatcatctg attctttttt gaggttataa
     5881 ataattaacc cccaaagagc tgtaaataca tacactatta gcgcaaaacc atagcttatt
     5941 tcaatggaaa ggatggctcc tgccataaac tgaaagaagc ttagaaggaa tatctgtatg
     6001 atctctctca tacccttggc actaaggaga cgaacaacct gcaaatatat taagaagttt
     6061 aatattccct ggaatacttc ttgtccaaaa tagacaatgc ttgccagaat gtataaaaca
     6121 acaacaaaag caataactgt ggatacggtc tgattgaact gatagttttt tttgtagtgg
     6181 tcattaataa agcttagaac tagtgataca taaaaaaccc ccattgtaag ggggctaacc
     6241 tggccggaaa tactgagagt aaaaaaacca agaaatgcta ttatgtgagt aaaaagtatt
     6301 agggcaaaag atattgtcat ttgagctgct ccgcatattc aagctcaagc tctcttagct
     6361 ttgttatttt atctcgatac tctgcagcct tttcatactg cagcttttta gatgcctttt
     6421 tcatgtcctt tgttaacagc tttaccatcg atggaatttt attaggagct acattatcta
     6481 attcaacgcc tatatccttt ggaacagtaa aataatcgta ctcaaatata gatgacaata
     6541 tatcacttat atttttctca atactttttg gagtaatgtt attagctgta ttaaattttt
     6601 cctgaatctc tcttctcctg taagtctcac ttatagccct ttgcatcgaa cctgtcattt
     6661 tatcggcata taatattacc ttgcctctaa catttcttgc agctcttcca aatacctgaa
     6721 taagtgaggt ttcagacctc aggtacccct ctttatctgc atcgagtata acgaccaaag
     6781 acacctctgg tatatcaaga ccttctctta acaaatttat accaataagt atatcaaatt
     6841 caccaagacg caggcttctt attattttta ttctctcaag tgcgtcaata tcagaatgca
     6901 gataatttac tcttagtccc aggtctttat aatattcact aaggtcctct gccatttttt
     6961 tagttagtgt ggtaattaat accctgtctc caatatctat tcgggattta atttcctgca
     7021 gcagatcatc aacctgattt tctgctggtt ttatttctat ctcaggatca ataagccctg
     7081 taggcctaat tatttgttca actacagcac cattagattt tgttatttca taatttgcag
     7141 gagttgctga tacatatatt gcactcctaa ccttagattc aaactcttta aaatttagtg
     7201 gtctgttatc tagggccgat ggcaatctaa atccatattc aaccagtgtt ttctttctgc
     7261 ttctgtcacc ttcatacatc cctcttatct gaggcattgt ttgatgactc tcatctataa
     7321 ctaccaaaaa gtcttcagga aaataatcta taagtgtcca tggaggctcc ccctcactcc
     7381 ttccggtaat atgtctggaa taattttcta tgccattaca aaaacccata gtcgttagaa
     7441 gctctaaatc ataatttgtt ctttcttcta ttcttctggc ttctaaatat ttattttctt
     7501 tttcaagtat tttaatcctc tgcttaagct cttctttaat agcttcaatc gcaattttaa
     7561 ttttgtcttg tgaggcaaca tagtgagagc ttggatatat tatagtctta tcaactttct
     7621 ttagggtttt acctcgtaat ggatcaatct cttttatgga ctcaattact ccatcaaaaa
     7681 attctaccct tatagcagta ttttcctgat gagatggaaa cacttcaacc gtatctccac
     7741 ggtacctgaa tgttcccctg gtcagatcat tattagttct ttgatactga acatctttta
     7801 gtttattaag cagtattttc ggatcgagcc ttccgccagt ttcaattgtt gtaagtagtc
     7861 cgtaataatc attgggtgaa cctattccat atatacatga aacgctcgat actataataa
     7921 tatctcttcg ttcaaataat gatgaagtgg cagcatgcct gagccggtct atcgtttcat
     7981 ttatagctac atccttatct atataggtat ctgtagttgg aatataagct tctggctgat
     8041 aatagtcata ataacttaca aagtagtgaa ccgaattatc cggaaataaa tccattagtt
     8101 caccgtagag ttgagcagca agcgttttat tatgtgcaat aattaaggta ggtctttgta
     8161 cattattaat tacgtttgca attgtaaaag tctttccgct tcccgtaact cccaggagca
     8221 cctgatgttt cttattggac gcaagaccgc ttgtaagttt ttctattgca cctggctgat
     8281 cgccctgggg cttgtattcg gtggtgaggt taaatagctt ttccaccaaa atagtattac
     8341 gtatttatta gaaaagttaa aataaaaggc aataattctt gttacttttt atgctttctg
     8401 ccagaccaca tttcatggag tttttttctt tggtcggggg ttaggacctc tcttatctca
     8461 agctgcatat ttctatgtat ttttccaagc tgtaatttta aaacatctac ctgttctgca
     8521 agattggata tctcctgttt atcagattga ggatctttta gtttctggtt aagctctttt
     8581 tgtttttgta taatgaattg ctttgtttca tgtaactttg ttttatgtaa attaaatatt
     8641 tgttctatcg tactaacctg cgcaggtgat aattgcagat tgcttactac atcactgttc
     8701 ttccaccatt tatacttagc tcccgcaaaa gccggaattt gagatatcat taaaatggtt
     8761 aatattaaat acagagtcct gtaaatcctc aaatctttat ccctcctttg attcctcttc
     8821 cagattgttc cagagtccgt atatttgcaa agactctgag ccttcaacag agataatatc
     8881 attaatttct tgtaaaagtc tattatcttg taaatcctgc tctgaatatg tcggattaac
     8941 agttgatgtt ctatacatat taactataaa aagactgcaa aataatgcca cggcaagcga
     9001 aggtactaaa aatgttgtcc ttataccttt tataaaacca atgatacctt tgttctcatt
     9061 cttttccttc tcttccatcc tgctccagaa tttactcaca tagttactct gtggttctat
     9121 ttcatcccat gtgtccatga gtctggtaag tttgttgttt ttagcaaagt agtccttgca
     9181 accattacaa ttaataagat gctcatcaat tgccacagac tcttccttgc ttaacacctg
     9241 cagcttaaaa tcatcataca tgcctttaat ctcatcacat ttcatccatt tccacctcat
     9301 ccaaataggg ttttaacttc tctattaatt tcatccgtcc cctatataca taagtcttta
     9361 cagctcccac tgtacagtcc aaagtttgtg caacctcttc atatgaaagc tgctcatact
     9421 tacaaagcat aattgccgat ctttcatttt cagaaagagt gttcatagct tcaaaaataa
     9481 tttcctttat ttcactccta tatcttacat ctgcctgatc cacagatttt atatcttcca
     9541 gtgaaccaac gttctcttgt atctcatcga ggctagaagc atgcttcctt gatcgggttg
     9601 aaacttctgt aaggcaaaga tttgttgcaa tcttatatag ccaggttgaa aacttagcct
     9661 ccggtctgta atttttaact gatctgtgta ctctcataaa tatttcctgt gttaagtcct
     9721 cggatgtttc ccgattatta gtatatctat acacatagtt caaaatctta ctgtagtatc
     9781 tctttaagat ttcagaaaat gcactttcat cgccgctttg aactctgagt attaaatcta
     9841 tatcgctttc catgtggcaa acatcttaca caatatttat aactataata attctaaata
     9901 gttacggata gttaaaaaaa ttatttcctt tatcaaatag ctgatttaaa atagctaagt
     9961 atcaataaat ttatggatat tagtatattt aaaataatct attaaataat gcaaaaacaa
    10021 ggaaaagtat acctgattgg cgcaggcccc ggtgatccag ggttaataac aattaatgca
    10081 gtgaaatgct taaactctgc agacgtgatt gtttatgatt atttagtaaa tactaaactc
    10141 ctagatcata gaaaacacaa ttccgaagta atttatgttg gcaaaaaaag cggacataaa
    10201 gaactgtctc aggaaaagat caacaagctt cttattaagc atgctaaaaa tggcaaaaca
    10261 gtcgccagac ttaaaggcgg cgaccccttt atatttggaa gaggtgctga agaagctatt
    10321 gaacttaata ataacaatat agattttgat ataattccag gagtaagctc tttttatgcc
    10381 gtccccgaat atgccggaat tcctccaacc cacaggaacg tttcatcgac aattgctgta
    10441 attaccggtc atgaagaccc gtcaaagaac agatcaaaca tcccttgggc ctcactgtct
    10501 aaaattagca ctgtcatatt tcttatgggt ataagaaatc tttcagagat cgttaaaaaa
    10561 ctcttaaaaa atagcaaatc ccctaacaca cctatcgcaa tcattacctg gggtacatat
    10621 ccaaatcaaa aaacgattac tggaaatctt gaaaatataa taaaaaaatc tcaggaagct
    10681 caaatagaat caccgggtat tattattgta ggtaaagttg ttaaatatcg tgaacagata
    10741 cagtggtttg aaaaaagacc actttatggg aaaaagatac ttactacaag accgtcttct
    10801 cagtcaaaat ttttcgttga gaaactaaat aatcttggtg cggaaacaat cgaatttccc
    10861 acaattgaga tttctgcacc gtcatcatgg aataaactcg ataaatcatt aaaacagctt
    10921 aagacaactg att
//
LOCUS       DBNGSV010000132        13573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1132767, whole genome shotgun sequence.
ACCESSION   DBNGSV010000132 DBNGSV010000000
VERSION     DBNGSV010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1132767"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="rfaE2"
                     /locus_tag="AC159U_04585"
     CDS             <1..1248
                     /gene="rfaE2"
                     /locus_tag="AC159U_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR3336368.1"
                     /translation="ELFGLIGNDDSGSLLKQLLKESKIQTKGIIVDKQRNTTTKSRII
                     AENQQVVRIDKENSQKVDSDLQDTLISKIKSSINKSPVDAVVFSDYNKGVITKKLVSE
                     ISRLTRLKNIFTIADPKGNDLIKYSGVNALTPNTKEASDLCGFNITDDRSLSKAARHL
                     TDKCRLDGVVITRGKDGISYRGKNKPLKTVSSNAKEVFDVTGAGDTVISSLLVSYLIS
                     NDWDFAAQTANSAAGAIVSRVGTSSLSQNELLRLIESGGDKEDKIQDIDSLKNQLFEH
                     KKKGRKIIFTNGCFDLLHPGHLMILKESKALGDILVVALNSDSSVRRLKGKSRPLISE
                     NERASIISSFDCVDYVTVFTEDTPLRIIEQTNPDVIVKGGDYSKKDVVGREHVEKHGG
                     EVVIIPILESFSTSSLVEKIKNS"
     gene            complement(1268..1567)
                     /locus_tag="AC159U_04590"
     CDS             complement(1268..1567)
                     /locus_tag="AC159U_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336369.1"
                     /translation="MAKTKDSFKTFKELFEKQFKTAFDSFDVNLKKAVGNIHLPTPKD
                     FLKLEKRVSELEKKVSRLEKPKKATTRKTTSSSSRTRKPAAAKATARSKRTSTKK"
     gene            complement(1575..2345)
                     /locus_tag="AC159U_04595"
     CDS             complement(1575..2345)
                     /locus_tag="AC159U_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336370.1"
                     /translation="MKKILISITALFVISTLLACGGASVNKSKDDKNLANQKQLAVSS
                     LKRKIYKQALEEIAVAEEIAPEDPEVHLIRGIILFAIQDLQGAEKSYKKAIEFNPEYS
                     EAHFNICGLYLHLERYDDAITHCKKATEDRLYKSRDRAYTAIGVAYFNMGDLDMAQKY
                     YEKSLKVNPSLVYTHNQMGKLYVAQGRLHDAIDEFETAIAGYQLYDEAHYNLGITYLK
                     LKEIFKACSSFKRVLEIAPNSDWGRKAKNYRKSACDGV"
     gene            complement(2350..3252)
                     /locus_tag="AC159U_04600"
     CDS             complement(2350..3252)
                     /locus_tag="AC159U_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3336371.1"
                     /translation="MSLLVVGSLGLDDIETPFGKVESVLGGSASYFSIAASKFTDVNV
                     VAVVGQDFPQAHLDLLSSKGVNINGIQKAEGDTFRWQGKYGYDLGDPETLGTYLNVFE
                     HFDPRIPDDYKQSDFVFLANIDPELQLNVLKQVDSPRLVACDTMNFWIENKKDALLEV
                     LKNVDILMINDSEARELGENSFITKAARNIMEMGPRILIIKRGEYGALKLSKKGIFWA
                     PSYPLEEVLDPTGAGDSFAGGFMGYLTNEYDITTTNLKRSVVYGCVVASFTVEDFSVN
                     RLAALKDEELNKRHSAFMNLTNIE"
     gene            complement(3249..4106)
                     /gene="mtnP"
                     /locus_tag="AC159U_04605"
     CDS             complement(3249..4106)
                     /gene="mtnP"
                     /locus_tag="AC159U_04605"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR3336372.1"
                     /translation="MEAIGVIGGSGLYEIEGLENIEYKDIDTPWGKPSDSFVTGTLNG
                     TEMVFLPRHGRGHKIMPSNINYRANIYGMKFLGVDSIISVSAVGSMKEEIPPGDVVLP
                     DQFIDFTKKRDSSFFGDGIVAHVSMADPVCPHLADSLHNVLQDMNAKVHKGGKYICIE
                     GPQFSSKAESELFRSWGVDIIGMTNMPEAKLAREAEICYSTLALSTDYDCWHDGHDNV
                     TVEDVIKILLQNTELAKEVIRNFVLAKSAEKKCNCNNSLRDAIITQPQAIDSESKEKY
                     KLLIGKYIK"
     gene            4285..5091
                     /locus_tag="AC159U_04610"
     CDS             4285..5091
                     /locus_tag="AC159U_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3336373.1"
                     /translation="MAFIATESFSQDLVIKLNGKQINQFDISELSKIIKPAEITIYEP
                     HEHKDRTYTGFPGYELLKAIYKQDLQSDKDIYITCTDGYKASITVTKFLTYDSYIVFK
                     SPGQTKFNLINKNQNNELVDLKPFYLIWDNIKYPELKKIGAYDFPYQIESIDLISFAQ
                     KFPNMSPPQKSSEQVNRGFVSYRKYCSTCHTINGEGGSKAVELNYPVNVTEYFNNKYL
                     RKWIDDPASIRYGTKMPRINPALDNRSNVIDDIIKYLKAMKKKKIRPELN"
     gene            complement(5083..5730)
                     /gene="rsmG"
                     /locus_tag="AC159U_04615"
     CDS             complement(5083..5730)
                     /gene="rsmG"
                     /locus_tag="AC159U_04615"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3336374.1"
                     /translation="MIIRDIFIQGTEEIGIILSAGDIEKFLIYLEELELWNKKINLTA
                     IKKDDDIITNHFLDSLAIVKYINPDSTLLDIGSGAGFPGVPVALSRPDCFVTCCDSAG
                     KKIMFINHIVRKLGLQNVKTISCRAGEGEQNLGFYDYVVTRAVSDLLTVIKLSKTYMT
                     ESGKIIAMRGEKGKDELEDVSGELNSLGLEIEKTDEFRLPHSGAKRVNIIFKKSV"
     gene            complement(5727..6521)
                     /locus_tag="AC159U_04620"
     CDS             complement(5727..6521)
                     /locus_tag="AC159U_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR3336375.1"
                     /translation="MKKNQLGYLIFTLLILGSSYFFTNKQSSSDYLVLKVIDGDTIVL
                     DDGSQTLVRFIGINSPEILTHESPGEPFSQQAKSFNEKLLAGKQVTIKFDKEKYDHYG
                     RMLGYIYADGVFVNEQLVRNGLATSLKIKPNTKHFDRILEAEEQARENKRGIWSAPGN
                     FIYPKENRQFYVKPQSAQRYLGQRVVTKGKITDVRKSEKVIVLKMDEDFDVVIFAGSW
                     KNFNHFGISPESDYLGFPVEVIGRIKKYKGRPQIIVSHPITLKKLL"
     gene            complement(6556..7329)
                     /locus_tag="AC159U_04625"
     CDS             complement(6556..7329)
                     /locus_tag="AC159U_04625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016426 - tRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HIR3336376.1"
                     /translation="MTIKEGDYVLILAPDDKTFLRKVNAEKQLSTHLGMINIKDIIGK
                     NYGDKIVSGLGNEFYLLEPTTWDKMMKVGRQTQIIYPKDASIIILKTGLSEGMRVVES
                     GTGSGSMTIALANTVKPGGKVYTYEKRDQFRENAMKNVETAGLSEYVEFNAGDARDGF
                     KETEVDVALIDLPSPWDGVPAAAKALRGGGRIASLSPTFNQIEENYKYLEEHGFTYLE
                     TVEVLVRNILVRAGKTRPSQRMISHTGFLTFGRKINKQE"
     gene            complement(7343..8503)
                     /locus_tag="AC159U_04630"
     CDS             complement(7343..8503)
                     /locus_tag="AC159U_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3336377.1"
                     /translation="MPLGLDKKGFINFSLITLANFFFFSNFSTFFLLPLYIKELGGTE
                     ADIGFIMGSFGITSLGAIPFVTYLIDKYGRKFFMFAGAVIMLLSSLLFLTVNELSPLL
                     YVYRLCQGVGFALFFTSATTSAADIVPMGKRAQGLGIFGAFTILAYSVGPTLGEFALN
                     NFGFDYFFLLASSFSIVAAVLILFSKVTDFQKSAERFGLGFFRLVVSKRYSLILLTNL
                     LLAGGLGSVLNFISAFLKEKEIGAYHFFIVYALTVTCVRIFGGRTSDIFGRKKVASPS
                     LIIFAISIACIVFINSGLIAVVVSFLFSFSYGMLYPVLTALVIDKAGPDERGKAMGAY
                     NACFSLGINFLAFPFGIIANNFGFNAMYLTSAFLVLISFFIFTLYYREEKIR"
     gene            8601..8673
                     /locus_tag="AC159U_04635"
     tRNA            8601..8673
                     /locus_tag="AC159U_04635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8634..8636,aa:Lys,seq:ttt)
     gene            8682..8759
                     /locus_tag="AC159U_04640"
     tRNA            8682..8759
                     /locus_tag="AC159U_04640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8718..8720,aa:Glu,seq:ttc)
     gene            9028..9516
                     /locus_tag="AC159U_04645"
     CDS             9028..9516
                     /locus_tag="AC159U_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3336378.1"
                     /translation="MKTPKNRPNYMELSGKLTKKLAEISQILNKSSLDNRMINLINIR
                     ASQINGCTFCNDMHVKEAKIEGERELRIYHLSTWKDSNLFTDKEKAVLEWTEAVTNIS
                     STSPSEEQYERIGTHLSQQEISDLTFAIAIINAWNRLATALSSQHGAVDYLYGLDRAG
                     LN"
     gene            9518..10006
                     /locus_tag="AC159U_04650"
     CDS             9518..10006
                     /locus_tag="AC159U_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3336379.1"
                     /translation="MILRNQVEWALHCVSLLAALPAEKRLSATDLAKFHGVPKEYLLK
                     ALQELSKAGIVDGSIGQKGGYKLARHPGEITFLDVIEAIEGKKSTFICTEIRQNSPCD
                     TPISALAKPCQVARIMYEADEAWRQVLKKTTIADLAKDVETELPEQFLTRIGQWIENN
                     IN"
     gene            complement(10057..10473)
                     /locus_tag="AC159U_04655"
     CDS             complement(10057..10473)
                     /locus_tag="AC159U_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3336380.1"
                     /translation="MKSYRKELWFNVPSRRGFINITPEVDKCLKESGISEGFVLVNAM
                     HITASVFINDDESGLHHDYDVWLEKLAPHEPVSQYKHNYSEDNADAHMKRQIMGREVV
                     VAITDGRLDFGPWEQIFYGEFDGRRKKRALIKIIGE"
     gene            complement(10657..11223)
                     /locus_tag="AC159U_04660"
     CDS             complement(10657..11223)
                     /locus_tag="AC159U_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336381.1"
                     /translation="METKTNKSFFRKFSKYFLVYFLFIFVAANFYLKHSNILIYSNAT
                     DDVAMLNIKWGMSPTEILFVTDTDFNHIIAEDPNHKSISQHLPLIDETNKSEINYYFV
                     NNQLYKVRIKENIATDTHDKVAKFIEKIGVNKNEKNIISDLYEGEKIRTENGRQKMSV
                     YIGFDAKDKPVNTYVTMYYKPIANKYEI"
     gene            complement(11468..12214)
                     /locus_tag="AC159U_04665"
     CDS             complement(11468..12214)
                     /locus_tag="AC159U_04665"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3336382.1"
                     /translation="MKESATENKIDIYSKDIPFIEGPRRRESELYTVIQIMTEFIKGF
                     RTFHFVGPCITVFGSARIAEDHPYYKMTMDIGARLAEMGLTVMTGGGPGLMEAANRGA
                     KEAGGRSVGCNILLPFEQKPNPYMDKWMDFQFFFVRKVMLIKYSYGFVVMPGGVGTMD
                     ELFQALTLIQTKKVINFPIVLIGKDYYKDVTELFDKMCEEQTISNNDRDLFLVTDSMD
                     QACEHIHVHAVEKFNLKTKKLPGRSKLLFE"
     gene            12377..13213
                     /locus_tag="AC159U_04670"
     CDS             12377..13213
                     /locus_tag="AC159U_04670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3336383.1"
                     /translation="MKSIVIVRQTPDTEAKINVASSGDSIDPEGIKWVLNPYDEFAVE
                     EAIRNKEKNGGEVVLITMGPARSLECLRTGLAMGADSAVHIKDDDFAFSDPFAIGKVI
                     AQEIKNIGEYDLILTGKKMIDEETGQVGIQVAQELGIPHVSIVTKLDINADAKTATAV
                     KDIEGGTMTVEVPLPSLITCEKGLNEPRYASLPGIMKAKKKPVKEVAAGDIDTGALGL
                     SAEALGKAGARIVNTKYEVPSIERKLTVLKGSQADMVKDAEVQSTAAEVVKLLREDAK
                     VI"
     gene            13248..>13573
                     /locus_tag="AC159U_04675"
     CDS             13248..>13573
                     /locus_tag="AC159U_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3336384.1"
                     /translation="MSEIWVVADLTLDGKVKSVTAEGLTVAKTKLSGGGGTVCGVLLG
                     SGVSGLAGELGKYGAEKVYVVDSDLLKDYTAEGYSAALADLIGQHNPDIVITGNSAAG
                     QDFFPRA"
BASE COUNT         4291 a         2788 c         2418 g         4076 t
ORIGIN      
        1 gagctttttg gacttattgg aaatgacgat agcggaagtt tgttaaaaca acttcttaaa
       61 gaatcaaaaa ttcagacaaa aggaattata gtagacaagc aaagaaacac caccaccaaa
      121 tccagaataa tcgcagaaaa ccagcaggtt gttagaatag ataaagaaaa ctctcaaaaa
      181 gttgattcag atttgcagga caccctgatc tctaaaatta aatcgtccat taataagagc
      241 ccagttgatg cagttgtatt ttcggattat aacaagggtg ttattacaaa aaaattagtt
      301 tcagaaatat ccagacttac gagacttaag aatattttta caatagccga tccaaagggt
      361 aacgatttaa taaaatattc aggcgtaaat gctctcacgc caaacacaaa agaagcttct
      421 gatctgtgtg gatttaatat cactgatgac agatcgctaa gtaaagcagc cagacattta
      481 accgacaagt gcaggcttga tggagttgtg ataaccaggg gaaaagatgg aatttcttac
      541 cggggaaaaa ataagccact taaaactgta tcttcaaatg ctaaagaggt atttgatgtt
      601 accggagcag gagatacagt tataagttct ctgctcgtat cttatcttat ctcaaatgac
      661 tgggattttg ccgcacaaac tgcaaacagc gcagcagggg ctatagtaag cagagtcggc
      721 acatcaagct taagccagaa cgagcttcta cgtttaatag aaagcggtgg agataaagaa
      781 gacaagatac aggatattga ctctcttaaa aaccagctgt ttgaacataa gaaaaaaggc
      841 aggaagatca tatttactaa cgggtgtttt gacctgcttc accccgggca tttaatgatc
      901 cttaaagagt caaaggctct tggtgatata ttagtagtag ctcttaacag tgacagctct
      961 gtaagaagac ttaagggtaa atccagaccg cttatatcag aaaacgaaag ggcaagcatc
     1021 atttcgtctt ttgactgtgt ggattatgtt acggtcttta ctgaggatac accgcttagg
     1081 atcattgagc agacaaaccc cgacgtaata gtaaaaggtg gcgattactc aaaaaaagat
     1141 gtagtgggca gggaacacgt agaaaaacat ggtggtgagg tggtaataat tccaatcctg
     1201 gagagttttt ctacctcttc acttgtcgag aagatcaaaa attcttaatg ccaaggactt
     1261 aagtattcta cttcttagta gaagttcttt tggatctggc cgtagctttt gctgccgcag
     1321 gttttctggt ccttgatgaa gacgaagttg tctttctagt agtagccttt tttggttttt
     1381 ctagtctact aaccttcttt tccagttcac ttacgcgttt ttcaagcttc aggaaatcct
     1441 tgggagtggg cagatgaata tttccaactg cctttttaag atttacatca aatgaatcaa
     1501 aggcagtttt aaactgcttt tcaaaaagtt ctttaaaagt tttgaatgaa tcttttgttt
     1561 tagccatatt gttattatac accatcgcac gcagattttc tgtagttttt agcctttctt
     1621 ccccaatcac tatttggagc aatctccaaa actctcttaa atgagctgca tgccttaaaa
     1681 atttctttta gcttcaaata ggttatacca agattatagt gagcttcatc gtacaattga
     1741 taaccggcaa tggccgtctc aaactcatct atcgcatcgt gcagtcttcc ctgagctaca
     1801 tatagttttc ccatctgatt atgggtgtac acaagagatg gattaacctt tagtgatttc
     1861 tcatagtatt tttgagccat atccaggtca cccatattaa aatacgctac ccctatggca
     1921 gtatacgctc tgtcccttga tttatacagt ctgtcttcag tagctttttt gcagtgggtt
     1981 attgcatcat catacctttc taagtgcaga tataatccgc agatattaaa gtgcgcttcg
     2041 gagtattcgg ggttgaattc aattgctttt ttgtaagact tttctgctcc ttgcagatcc
     2101 tgaattgcaa aaagaataat tcctcttatg aggtgaacct cagggtcttc aggagctatt
     2161 tcttcagcaa cggcaatttc ttctaaagcc tgtttataaa tttttctctt aagtgatgat
     2221 acggctaatt gtttttgatt agcaagattt ttatcatctt tggacttgtt tacagaagct
     2281 ccgccgcaag ctaaaagtgt tgatatcaca aatagtgctg taattgatat aagtattttt
     2341 ttcatatttc tactctatgt ttgttaaatt catgaaggca ctatgtctct tatttagttc
     2401 ttcatctttc aatgcagcca gtctgtttac actaaaatcc tctacggtaa aagaagccac
     2461 tacacagcca taaacaacag acctcttaag atttgtggtc gttatatcgt attcgttagt
     2521 aagatatccc ataaacccac cggcaaagga gtctcccgcc ccagtaggat caagcacctc
     2581 ttcaagagga taactaggcg cccaaaatat tcctttttta gaaagtttaa gagcaccgta
     2641 ctcccctcgc tttattatca atatcctggg tcccatttcc ataatatttc tggcggcttt
     2701 ggttataaaa gaattctcac caagctccct tgcttcagaa tcatttatca taaggatatc
     2761 cacattctta agtacttcta aaagcgcgtc ctttttgttt tcgatccaga aattcatcgt
     2821 atcacaggca acaagcctgg gactatccac ctgctttaac acatttaact gcaactctgg
     2881 atctatatta gctaaaaaca caaaatcaga ctgtttataa tcatccggga tacggggatc
     2941 aaaatgctca aatacattta gatatgtacc tagcgtctca ggatcaccaa gatcataccc
     3001 atacttgccc tgccacctga atgtatcgcc ctcagctttt tgtataccgt ttatgtttac
     3061 tcccttggag cttagtaagt ctaagtgggc ttggggaaaa tcctgcccaa ccacggctac
     3121 cacatttaca tcagtaaact tgcttgcagc tatagagaaa taagaagccg acccacccag
     3181 caccgactct acttttccaa acggggtttc aatatcatca agacctaaag aacctactac
     3241 cagtaaactc atttaatata ttttcctata agtaatttgt atttttcttt tgattcacta
     3301 tctattgcct ggggctgagt gattatagca tcccttagtg aattattaca gttacacttt
     3361 ttttctgcag acttggccaa tacgaagttt cttatcactt cctttgctag ctcagtattt
     3421 tgaagcagaa tctttattac atcttccacc gttacgttgt catgaccatc atgccagcag
     3481 tcatagtcag tagacagagc gagagtagaa tagcaaattt cggcctcacg cgcaagcttt
     3541 gcttcgggca tgttggtcat tcctattata tccacccccc agcttctaaa taattcactc
     3601 tcagccttag aagaaaactg cggcccctca atacatatat acttaccacc cttatgcact
     3661 tttgcattca tgtcctgcaa aacattgtga agtgagtcag caagatgagg acaaacagga
     3721 tctgccatcg agacgtgtgc cacaataccg tctccaaaaa agctggaatc tcttttcttg
     3781 gtaaagtcta taaactgatc cggaagtacg acatcacccg gtggaatctc ctctttcata
     3841 ctgccaacag cacttacaga aataatgctg tccactccaa gaaatttcat tccgtagata
     3901 tttgccctgt agttaatatt ggagggcata attttatgac cccttccgtg ccttggcaga
     3961 aataccattt cagtaccgtt tagagttcct gtgacaaagc tgtcagatgg ttttccccat
     4021 ggggtatcaa tatccttata ttcaatattt tccaggcctt ctatttcata aagcccgctt
     4081 ccgcctataa caccgattgc ttccatatat atttaactcc tcttaagtac ttacaaacca
     4141 gataaacgca ataaagataa caagaaaaaa tatattgtag aattttatgg gatataatat
     4201 ttactctatc tggattaaaa ataaaattat taatctaaat tattacataa aatgcagaga
     4261 atagttttat gggccttaat aatagtggct tttatagcca ctgaaagttt ttcgcaagat
     4321 ctggttatta aactaaacgg aaaacaaatc aatcaatttg atataagcga gctttctaaa
     4381 attataaaac ctgctgaaat tacaatttat gagccgcacg aacacaaaga tagaacatat
     4441 accggatttc caggttatga gctgctaaaa gctatatata agcaagatct gcaatcagac
     4501 aaagacattt acatcacatg tacagacggc tataaagcct ccatcacagt tacaaaattc
     4561 ttaacctacg acagttatat tgtctttaaa tcccccggac aaacaaaatt taatctgatt
     4621 aataaaaatc agaacaatga actggtagac ctaaaaccgt tttatttaat ttgggataac
     4681 attaaatatc ccgaactcaa aaaaataggc gcctatgatt tcccctatca gatagaatca
     4741 attgatctca taagttttgc acagaagttt cccaatatgt ctcctcctca aaaaagcagc
     4801 gaacaggtaa acaggggatt tgtatcatac agaaaatatt gttctacttg tcatactata
     4861 aatggtgagg gtgggagcaa agctgttgag cttaattacc cggttaatgt gacagaatat
     4921 tttaataaca agtaccttag gaaatggatc gatgatccgg cctctattcg ctacggcact
     4981 aaaatgccta gaataaaccc tgcgctagat aacaggtcta atgttataga cgacataata
     5041 aaatatttaa aagcaatgaa aaaaaagaag atcagacctg aattaaactg attttttgaa
     5101 tattatatta actcttttag ctccgctatg gggcagccga aactcatctg ttttttctat
     5161 ttccaaaccc agactattca actcacccga tacatcttct aattcatcct ttcccttttc
     5221 acccctcata gctataattt tgcctgactc tgtcatatat gttttgctca gttttattac
     5281 ggttaataaa tcagatacgg ctcttgtaac aacatagtca taaaatccca agttctgctc
     5341 accttcccct gccctacaag aaatagtttt aacattttgc agccctagtt tacgcacaat
     5401 atgattaata aacattattt tctttcctgc cgaatcacag caggttacaa aacagtcagg
     5461 acggctaagc gccacaggca ctcccggaaa tcccgcaccg gagccaatat caagaagtgt
     5521 agaatccgga ttaatatatt taactatagc aagagaatct aaaaaatgat tcgtaattat
     5581 atcatcatct ttttttatag ccgtaagatt tatcttttta ttccatagtt caagctcttc
     5641 taagtatatt aaaaatttct ctatatcacc tgcacttaga attatgccta tttcctcagt
     5701 gccctgtata aagatatcgc gaataatcat agcagttttt taagtgtaat cggatgactt
     5761 actattatct gtggcctgcc cttatatttt ttaatccttc caattacctc tacgggaaat
     5821 cctaaatagt ccgattcagg agatattcca aaatgattaa aatttttcca gctgcctgca
     5881 aaaataacga catcaaagtc ctcatccatc tttagcacaa ttaccttctc tgacttcctc
     5941 acatctgtaa tttttccctt cgtaacgacc ctctgcccca ggtatctctg cgcactttgg
     6001 ggttttacat aaaactgcct gttttccttt ggatatatga aattgccggg tgctgaccaa
     6061 attccccttt tattttccct ggcctgttct tctgcttcta gaatacggtc aaaatgcttt
     6121 gtgtttggct taatcttaag tgaagtagca aggccgtttc ttaccaactg ctcatttacg
     6181 aaaacaccat cagcatatat ataaccaagc atccttccgt aatgatcata cttttcctta
     6241 tcgaatttta ttgtaacctg tttgccagca aggagctttt cattaaaact cttagcctgc
     6301 tgagaaaacg gttccccggg tgattcatga gtaagaattt cagggctgtt aatgcctata
     6361 aaacgaacga gagtttgaga gccgtcatca aggacaattg tgtcaccatc aatgactttt
     6421 aaaaccagat aatctgaaga gctttgtttg tttgtaaaga aataggaaga gccgagaatt
     6481 aaaagagtaa agattaaata acctaattga ttcttcttca taaatattta ttgcgcaaat
     6541 atggtctaaa actgcttatt cctgtttgtt tatctttctt ccaaaggtaa gaaagcccgt
     6601 gtggctaatc attctctgtg atggtcttgt cttacccgct ctaactaaaa tattccggac
     6661 taacacctct actgtttcta aataagtaaa cccgtgttcc tctaaatatt tgtagttttc
     6721 ttcgatctga ttaaaagtag gagacagact tgctatacgc cctccccctc taagagcctt
     6781 agccgcagcg ggtactccgt cccatggaga aggaagatct attagggcta catcaacttc
     6841 agtttctttg aaaccatccc ttgcgtcccc tgcattaaac tccacatatt ctgaaagccc
     6901 ggcagtctcc acatttttca ttgcattttc tctaaactga tctcttttct catatgtata
     6961 gacctttccg ccaggcttta cggtatttgc aagcgcaatt gtcatagacc ccgaacccgt
     7021 accgctttcc acaaccctca ttccctctga aagccccgtt tttagtataa tgatcgaggc
     7081 gtcttttgga tagatgatct gggtctgcct gcccactttc atcatcttgt cccaggttgt
     7141 tggctctaag agataaaact cattgccaag ccctgaaaca attttatcac cgtaattctt
     7201 cccgataata tctttaatat taatcatacc cagatgggtg ctaagctgtt tttccgcatt
     7261 tacctttctt aagaatgttt tatcgtccgg agcaagtata agtacatagt cgccttcttt
     7321 tatcgtcata ttaatattcc cattacctga ttttttcttc tctataatat agtgtaaaaa
     7381 taaaaaatga tataaggacc aaaaatgccg aggttagata catagcattg aacccgaagt
     7441 tattggcaat gatcccaaaa gggaaagcca gaaagtttat gccaaggcta aagcacgcat
     7501 tatatgcccc catagcttta cccctttcat ctggccccgc tttgtctatc acaagagcag
     7561 ttagaaccgg atacaacatt ccgtagctaa agctgaagag aaatgaaacc actactgcta
     7621 taaggccgga gtttataaaa acaatacatg ctattgatat cgcaaatata attaaacttg
     7681 gtgaggcaac ttttttcctg ccgaatatat cggacgtcct tccaccaaat atcctcacac
     7741 atgtaacggt aagcgcatag actataaaaa agtggtatgc gccaatctcc ttttctttta
     7801 aaaatgcaga aataaaatta agaactgaac caagccctcc tgcaagcaaa aggttggtta
     7861 gaagtattag tgaataccgt tttgaaacaa caagcctgaa aaacccaaga ccaaaccttt
     7921 ccgctgattt ttgaaaatcc gttacttttg aaaacaggat caaaactgca gcaacgatac
     7981 taaaagatga agccagtaaa aagaagtaat caaaaccaaa gttatttagg gcaaattctc
     8041 caagagtggg gcccactgag taggcaagta tggtaaaagc gccgaatatg ccaagaccct
     8101 gcgctctttt tcccataggc actatgtctg cagcagatgt ggtagcagag gtaaaaaaca
     8161 gggcaaaccc tactccctgg cacaaccggt atacatatag caagggactc agttcgttta
     8221 cagtcaaaaa gagaagggat gagagaagca ttatcacagc cccggcaaac ataaagaact
     8281 ttcttccata cttatctata agataagtaa caaacggaat tgccccaaga gaagtgatac
     8341 cgaaagaacc catgataaaa ccaatatctg cctcagttcc gccaagctcc ttaatatata
     8401 aggggagcag aaagaacgtg gaaaaattgc taaagaaaaa gaagttagca agtgtgatga
     8461 ggctaaaatt tataaaccct tttttatcga gtcccagtgg catattattt gtatattatt
     8521 atagataaaa agggttagaa tatatgataa tttagccctt tgacaacaat attaagcgtg
     8581 atatatttgt tcaccttatt gagccgttag ctcaggtggt agagcaactg ccttttaagc
     8641 agtgggtcgc aggttcgaat cctgcacggc tcatattgtg tgtccccgtc gtctagcttg
     8701 gcccaggaca ccggcctttc acgccggcaa caggggttca aatcccctcg gggacgccat
     8761 tattaaatgc tataaccaga attgcttctg tatccattat cagaaagctg tcccacctat
     8821 gagaactttc aaacaaagct taaaagtcat aactatttta attttcatct attaatatgc
     8881 tttttcataa tctgtatagc tactacttca ctaacatact gtagcgtttt atcatttcag
     8941 tattcttctt agtcagattc cattgacaac aatttcaata cagatataat atatctgtat
     9001 tgagaaacac aaattaagga tcacaaaatg aaaacaccaa aaaatcgtcc aaattatatg
     9061 gagctatccg gtaagctcac aaagaagtta gccgagatca gccagatact taacaaaagc
     9121 agtctggaca atcgaatgat taatcttata aatatcagag cttcgcaaat taacgggtgt
     9181 acattttgca acgacatgca cgttaaagaa gcaaagattg agggtgaaag ggagcttaga
     9241 atttatcatt tatcaacctg gaaagactca aacctgttta ccgataaaga aaaagccgta
     9301 ctagagtgga ccgaggcggt taccaatatc tcgtctacct caccaagcga agagcagtat
     9361 gaaagaatag gtacacatct gtcccagcag gaaatctccg atcttacgtt tgctattgcc
     9421 attattaatg catggaaccg tcttgcaact gccctatcat ctcagcatgg tgctgtcgat
     9481 tacttgtatg gactggaccg tgcaggacta aactaaattg atattaagaa atcaagtaga
     9541 atgggcttta cactgcgttt ctttgctggc agctctgcct gccgaaaaaa gactctcggc
     9601 aacggatctg gccaaatttc atggtgtgcc caaagaatat cttcttaagg cactgcagga
     9661 actatcaaag gccggaattg tggatggaag cattggtcag aaaggaggct acaagttggc
     9721 cagacatccc ggcgagataa ctttcctgga tgtaattgaa gctattgaag gcaagaaatc
     9781 gactttcata tgcaccgaga taagacaaaa ttctccatgc gatacaccaa tatcagcatt
     9841 ggcaaagcca tgtcaggttg caagaatcat gtatgaggct gatgaggcat ggaggcaggt
     9901 tcttaaaaaa actacgattg cagatttagc aaaggatgtt gaaaccgaac tacctgaaca
     9961 gtttcttaca agaatcggtc agtggattga gaataatatt aattaaatta atcaatagac
    10021 tatcgttaaa actctaaatt taatttagat gacaatttat tcgccaataa ttttaataag
    10081 cgccctcttt ttccttctac cgtcaaactc gccatagaat atctgctccc atgggccaaa
    10141 atcgagcctc ccatctgtaa tagcaaccac tacctctctt cccataatct gtcttttcat
    10201 atgagcatcg gcattatcct cagaataatt gtgcttatac tgtgatacag gctcatgcgg
    10261 ggcaagcttt tcaagccaca cgtcatagtc atggtgaagg ccggactcat catcattaat
    10321 aaaaaccgag gctgttatat gcatcgcatt tacaagcaca aatccctcag aaatgccgct
    10381 ctcttttaag catttatcaa cttcaggggt tatgttaata aatccacgtc tgcttggaac
    10441 attaaaccat agctcttttc tataactttt catagcaatt ctccactttt tcaacaattt
    10501 ctagtttaac tatattctaa agacaaacta attgcttgga aagttttatt acgggtttaa
    10561 tacctgggaa gaaaataaaa aaaaagtggg ttacatttct gaaaccctct tttgattaaa
    10621 gatacgagtt gatatggtaa ctttatttat tattggttat atctcatatt tattagctat
    10681 tggtttgtag tacattgtta cataggtatt taccggcttg tccttggcat caaatccaat
    10741 atataccgac attttctgtc taccattctc ggttctaatt ttttcaccct catatagatc
    10801 cgagataata tttttttcat ttttattaac gcctattttt tcaataaact ttgccacttt
    10861 atcatgagta tccgttgcaa tgttttcttt tattcttact ttatacagct ggttatttac
    10921 aaaatagtaa ttaatctcag atttatttgt ctcatctatt aatggaagat gctgtgaaat
    10981 acttttatgg ttagggtctt cagcaattat atgattgaaa tcagtgtcgg taacaaaaag
    11041 tatctcagtc ggtgacattc cccatttaat attaagcata gcaacatcat cggtagcatt
    11101 tgaataaatc aaaatattag aatgtttgag atagaaattc gctgctacaa aaatgaacag
    11161 gaaatatact agaaaatatt ttgaaaattt tctgaaaaat gatttattgg tttttgtttc
    11221 catacttgct ctcctaaaat atgttttaca tagaatatag caatgagtgt gccaaaagca
    11281 ggttatttgg catttatgga tcaaaaaagg gctatttcaa tcaaatttca aaccttggta
    11341 gtacaaattt catacaaaat cacattgata cgataagggg tttaagacat caaagagaat
    11401 ataagaatga aatcaggaaa atttttgtta actatttgga aacgtattcc ctaaatggta
    11461 caaaaattta ctcaaaaagc agttttgatc ttccgggaag ttttttggtc ttaagattaa
    11521 atttttccac agcatgaaca tgtatatgct cacaggcctg gtccatagag tcagtaacta
    11581 aaaacaggtc tctatcatta ttgcttatag tttgctcttc acacatttta tcaaacagtt
    11641 cggtaacatc cttgtaataa tccttaccta tcagaacaat cgggaaatta atcacttttt
    11701 ttgtctgtat taatgtgagc gcctgaaaaa gctcgtccat cgtgcccact cctccgggca
    11761 taacgacaaa accgtaagaa tactttatta acatcacttt tcttacaaaa aagaactgaa
    11821 agtccatcca tttatccata tatggatttg gcttctgttc aaagggaagc agaatattac
    11881 accctacaga ccttcctccg gcttcctttg cccccctgtt tgcagcctcc ataagcccag
    11941 ggccgcctcc tgtcattacg gtaagtccca tttctgcaag ccttgcccca atatccattg
    12001 tcatcttata gtatggatgg tcttcagcaa tgcgcgcaga gccaaacaca gtaatacaag
    12061 ggccaacaaa atggaatgtt ctaaaaccct tgataaattc ggtcataatc tgaatgaccg
    12121 tatacagctc actctctctt ctccgtgggc cttcaataaa aggaatatcc ttagaataaa
    12181 tatctatttt attttccgta gcactttctt tcatattact ccctttttgg ctgtttttag
    12241 gttattaaag atttattact ctctatattg aaaagattca agtagatgct acaataatgc
    12301 ggttttttgt cctgaagatc aaatttctaa aataattttt cttagggatt atcaaaaaag
    12361 taggaggtaa tggaagttga aaagtattgt aatcgtcaga caaacacctg acacagaagc
    12421 caaaatcaat gttgcttcca gtggagattc aattgatccc gaagggatta aatgggtact
    12481 gaacccatac gatgaattcg cagttgaaga agccattagg aacaaagaga aaaacggagg
    12541 cgaagtagtt cttatcacaa tgggtcctgc aagatctctt gaatgtctaa gaacaggtct
    12601 tgctatggga gcagattctg ctgttcatat taaagacgac gactttgcat tttccgatcc
    12661 atttgcaatt ggaaaagtaa tagctcagga aatcaaaaac atcggagaat atgaccttat
    12721 ccttaccgga aagaaaatga tcgacgaaga aacaggacag gttggaattc aggtagctca
    12781 ggagcttgga attcctcatg tatccattgt aacaaaactg gacatcaacg ccgatgctaa
    12841 aacagccaca gctgttaaag atatagaagg cggaacaatg acagttgagg taccactacc
    12901 ttctctaatt acatgtgaaa aagggttaaa tgaacccaga tatgcttcac tcccaggaat
    12961 tatgaaagcc aaaaagaaac ctgtaaaaga agttgctgca ggcgacatag atacaggcgc
    13021 tctcggactt tccgctgaag cacttggaaa agcaggtgca agaatcgtta acactaaata
    13081 cgaggttcca tcaatcgaaa ggaagctcac ggtgctcaag ggctcacagg cagatatggt
    13141 aaaagacgca gaggttcaga gcacagctgc cgaggtagta aaacttctaa gagaagacgc
    13201 caaggttatc taattttgaa taaaaggtat ttatcaggag gaatacaatg agtgaaattt
    13261 gggtagtagc agatctaaca ctggacggca aagtaaaaag cgttacagcc gaggggctta
    13321 cagttgcaaa gaccaagctt tcaggaggcg gcggcacagt ttgcggagtg cttttgggct
    13381 ctggtgtatc gggtcttgca ggagaactgg gaaaatacgg agctgaaaaa gtttatgtag
    13441 ttgacagtga tcttctaaaa gactacacag ccgagggtta ttcggcagcg cttgctgatc
    13501 ttatcggcca gcacaacccc gatatcgtta tcacaggaaa ctcagccgct gggcaggatt
    13561 tttttccaag agc
//
LOCUS       DBNGSV010000133        11360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1548560, whole genome shotgun sequence.
ACCESSION   DBNGSV010000133 DBNGSV010000000
VERSION     DBNGSV010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1548560"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..577)
                     /locus_tag="AC159U_04680"
     CDS             complement(188..577)
                     /locus_tag="AC159U_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336385.1"
                     /translation="MKKVLSFFLALNIFLCYGGLCDLCHALGPEPDTAADCHSMGAMQ
                     MEQSTIDTSLDFQISSIEENISLCQYFLLSKSFEIEDNLYDGEIFTELFISQTNNYNY
                     IFEKSSFKIPDRAIPTELFIKNSSYLI"
     gene            complement(679..1257)
                     /locus_tag="AC159U_04685"
     CDS             complement(679..1257)
                     /locus_tag="AC159U_04685"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3336386.1"
                     /translation="MTTDNKPRCSWMGDDEIMLHYHDEEWGVPVHDDRKWFEYIILDA
                     FQAGLSWKTVLHKREGFKKVFFKFDPIKVARITDKQMESARNNPAIIRNRLKIKAAKT
                     NAEAFLKLQKEHGSFNSYIWSFTDGQVIQNKWNSLGDIPASTPLSDEISKALKKEGFT
                     FVGSTICYAFLQAAGVVNDHITSCFRYKELIK"
     gene            complement(1318..1614)
                     /locus_tag="AC159U_04690"
     CDS             complement(1318..1614)
                     /locus_tag="AC159U_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336387.1"
                     /translation="MAQFIDNGSFTIKGFKLFWKIRHWGGASNAYENHRGIAVCVNAE
                     SGKERELIVEFPFNMYFFEKPRSQRDFEAKLFQEVELALDQGWDPKSRGKAKTW"
     gene            complement(1690..2340)
                     /locus_tag="AC159U_04695"
     CDS             complement(1690..2340)
                     /locus_tag="AC159U_04695"
                     /inference="COORDINATES: protein motif:HMM:NF046610.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HIR3336388.1"
                     /translation="MNHKLISRLQDSYEKKKPLIKSRLNDFKQIRKNASDELLFEEMT
                     FCILAAGTSAKMALNSVEAVKDVLAFGDFNQIRASLIGHYRYPNLRANHIIQCREYLK
                     SEYNFNIRDMIANIKDPIERRGFLATTSEIKGIGFKEASHFLRNTGHSGYAILDKHII
                     YYLYELNLIRSNEIPTTENKYLETEKILINFSKDIGINIDELDLLLWSEKTGEILK"
     gene            complement(2371..4224)
                     /locus_tag="AC159U_04700"
     CDS             complement(2371..4224)
                     /locus_tag="AC159U_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3336389.1"
                     /translation="MINFSSMFTRYYYLFLPILLLTISCATTGKQASQSISDNAFSAL
                     SNQSDSYYGGHVLGQSEASAEQYFVREDRTGYYLLDPDDQTWKAHYYFFRIRDHIYED
                     LVDQLPTTDQEIPIVLNDKVQKYINFYQNRGRKTFTRWLERSGKFIPMMTEILKKKDL
                     PSDLVYLSMIESGFNVKARSPMGAVGPWQFIRGTGRKYGLTINSWIDERMNPEKSTIA
                     AANYLSDLYEMFESWELAAAGYNAGEHRVQRAIDKYNHDDFWEMSRTRKALPRETQEY
                     VPKIVAALVIVKNPEKYGFTEIDYHKPQSYEIAVVPAQKSLKDIARVIKTDYKTLRDL
                     NPSLIRNATPPGSNYLLKVPDGYGQIVGEKQTELYALKKYNAPMYLTHRVKSGDTLGG
                     IARRYRSSVSSIKRTNKLRGNLIRIGQKLRIPTKYTGSSYASNSSSSKSSSYRASSKS
                     STYRVRKGDTVGGIASRHGVSIKSIKAANKIKGNTIYPGQKLTIPGGSSYSSKSTTVA
                     KKAKTYKVRSGDTLSQIAERHGVRLHSLKRENNIKGANIRVGQVLKIPTKVYAKSSSS
                     KSNLISYNVRSGDNLWEIARKHNTTVSKIKKWNNLKSNRLDRGDVLKIYVK"
     gene            4362..5666
                     /locus_tag="AC159U_04705"
     CDS             4362..5666
                     /locus_tag="AC159U_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336390.1"
                     /translation="MEVPDGFETALNFGFYDSIFSRRSRRFGLGMEIEEGTLKHKSSH
                     KPVALSNLEEAMLIWAGTGMTGLSLGDLPRTGISWLFHWTGRSWPCSCNSHSTELFYT
                     NDDGVYMVKLFDLLPDKDQTSIFQNKSDEQKLERLLSLFNKSQLKLEDGRAVLPKGEP
                     GLFDFNAWNVNKPGTTFLIPVTNTTVEYMVLLFIYFGSKYGFNIIDELNGGRSCGLDK
                     WIKKGYIKNDVRMSLFDLELRVLTTLNVEQAFICQNMNLALQCLGLGGWAFTGLLPHY
                     ALGLKPEFPGLGFTFTTPEESLRSEKNPVPVGRDGVFEALCPPYVKDMGEAVDKFLQI
                     RGDFWNEDNPFPYQDPEGILDDEYHPKEVRVEIVKDFCRYVHENYGRFPAFLDPMFTR
                     LVFQAHHLDLEFYDKHYKEGAYTELHKDHFKLWHPDMGDPFG"
     gene            complement(5682..6254)
                     /gene="efp"
                     /locus_tag="AC159U_04710"
     CDS             complement(5682..6254)
                     /gene="efp"
                     /locus_tag="AC159U_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3336391.1"
                     /translation="MGVVDVSRFHKGLKIEFEDSVWEVTDYQHSKMGRSGAIITTKLK
                     NIFTGAAQEKKFRSGDTFQTPDLEKKQMQYLYKDDISFHFMDNETYDQIAISPDQVGD
                     SAKFLKEQQDVIVNYYNGEPIGIELPTTVDLEVTETDPGLKGDTVSASTKPATVETGA
                     VINVPLFIDIGEIIKVDTRDGSYVERVKSK"
     gene            complement(6285..6467)
                     /locus_tag="AC159U_04715"
     CDS             complement(6285..6467)
                     /locus_tag="AC159U_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3336392.1"
                     /translation="MKVKCPTCNKSVNWEDTIYRPFCSERCKMIDLGTWANEQYSMPV
                     EDAIDNEELEEHEQVH"
     gene            complement(6532..7119)
                     /locus_tag="AC159U_04720"
     CDS             complement(6532..7119)
                     /locus_tag="AC159U_04720"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR3336393.1"
                     /translation="MKEHVISALRESADVKMQMAKESLDEIVQVSELITRALKSGKKV
                     LIFGNGGSAADSQHIAAEFVNRFMIDRKALAAIALTTDSSIITSIANDTSFNDIFSRQ
                     IEAIGNKGDIALGITTSGKSANILKALEIAKEKELKTVAFSGSGTTKLKKHTDHIIAV
                     PSTSTQRIQEAHITVAHIICELIENALTESGSSGK"
     gene            complement(7123..8946)
                     /gene="mutL"
                     /locus_tag="AC159U_04725"
     CDS             complement(7123..8946)
                     /gene="mutL"
                     /locus_tag="AC159U_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3336394.1"
                     /translation="MGIITILPDDVVSKIAAGEIVERPASVVKELIENSLDANSKKIK
                     ITLVSGGKKFISVEDDGIGMNRDDALLCLERHATSKIKAADDLLHVSTLGFRGEALPS
                     ISSISKFRLVTKQDQQLSGCEIVIDGGVIRKVNDTGCPEGTLIEVRSLFYNTPPRLKF
                     LKKIETELSNVLDIVQRHAIIRYDVSFEVIHNDKTILNLSSRSNLHSRLLEIYPKVDL
                     HEVSAESSGVVVHGFLSSPNDVRTSTYKLFCYVNGRPVKDKLITRSVISSFGKVIEKG
                     RFPQGVLFTQLPSSEVDINVHPTKSEVRFHSAYKVSELISSAISKMLENAPWIMAFDK
                     KPYSNTNIDQKNINNTYNYRPADTRRVSPNYFQSEDQLRVLREIGQDYKQDYKIESSP
                     QVGSEEILTAQSQSDDIFTQKGFFGSLNIVGQINNLYIVCESQSGMILIDQHAAHERV
                     NYEKFKKQFETSTLQTQKFLFPEVIELTGAESTVVDDNIDLFSKMGFDLEKFGDWSYL
                     LRCSPVFLKNGDWHKIIVDIIGELKENDAEKSVSDRIDLLIATIACHKSVTANQYLQT
                     EKLQFLLDELDKTDLPHFCPHGRPVSKEFTYGEMEKLFKRT"
     gene            9130..10878
                     /locus_tag="AC159U_04730"
     CDS             9130..10878
                     /locus_tag="AC159U_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336395.1"
                     /translation="MKMFNIYKIGLLTIAVLLLATLIIPTDAKSQQKKRKKESAKTAL
                     IITDYEWKTGGRGIEGILNSVTIENVGSKDYENISIEVELISNNDVPQGSLRATIKDN
                     LISGSTKTFTNISLGFMNTDLEKSIIRITGAELVERGIDSPKDVILVKDWQWLETNYG
                     TEGILKYITLENKSDINFKKIQITINFQDPADSSTVPYRTIIHDYLPANSEKTFNNIN
                     VGFKDRGARSAVITVRDATKITKREYRSLLAKEGIRVDENTPGIDSYPDYDGQGERDR
                     NLSLAERYRKYVLKVDPDSGEQIIEGIEDSEGELQKEILIEEPQDTSPYYRDRNQYAT
                     RVDNAISDEQLLREQAKIFPEVFQEIVIVSAEADTFETDTRTESYSQPVSEPQTHERH
                     KIEYRYEKPKEKKNVISRAFGWSKNLVGLGDDEEEEYELTETKVAVDNTNTASTKRHP
                     YTSVARVDGKTVEYENISEDEYLIESDEDYEPLPTIDIEIRDYKVKPGIVSTIGQLSE
                     ITLVNRSNITYTNIQLEVTFYNRGATKQLGSNKFVINEILPKRTRRTFNNVDIGFLNT
                     VPEELKIRIVDAIDIN"
     gene            complement(11011..>11360)
                     /locus_tag="AC159U_04735"
     CDS             complement(11011..>11360)
                     /locus_tag="AC159U_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HIR3336396.1"
                     /translation="INLLYTFFKYNLYLDFYKSFVIIEAQFSNSVLIGRTRGGGIMER
                     VEKEHLSKHIDIESKRFFFDVKENHKGKYLRITELSGGRSCIVIPLGGIKTFKDRLGE
                     IIDETDEIVGLES"
BASE COUNT         3482 a         2199 c         1982 g         3697 t
ORIGIN      
        1 tctgattgcg ctatcgagac taagactctc ctttgcctca agcacggcag aatttataaa
       61 taaaatcagt gttaaaataa gatagtaata catatttccc tccacggtta taaattagat
      121 tccataaaag aacccaagtg gacaaaactt ccctgtaaac gggaaaaaat aacggagaga
      181 aatatttcta gattaaataa gaagagtttt ttataaaaag ctcggtgggt attgctctgt
      241 caggtatctt aaaactagat ttctcgaaaa tataattgta attattagtc tgtgaaataa
      301 ataactctgt aaatatttca ccatcataca aattgtcttc aatctcaaaa gatttactta
      361 aaagaaaata ctggcaaaga gatatatttt cttctataga tgatatctga aaatccaaag
      421 atgtatctat ggttgattgc tccatctgca ttgcgcccat ggaatgacag tcagccgcag
      481 tatccggctc agggcctaac gcatgacaca gatcacacag cccgccataa caaaggaata
      541 tatttagagc aagaaaaaat gatagaacct ttttcatatt agttttaata taaagttttt
      601 aatggtaaaa agcaatacaa ttaaatttaa catataacat tcaagcataa attatttcat
      661 gatgcaggtg ggttacagtt attttattaa ttccttatac ctaaaacagc ttgttatatg
      721 atcatttacc acgcctgctg cctgtaggaa tgcataacaa atagtagatc ccacaaaggt
      781 aaatccctct ttttttagcg ccttgctaat ttcatctgaa agaggggttg atgccggaat
      841 atcaccgaga ctattccact tattttgaat tacttgacca tctgtaaatg accatatata
      901 tgaattaaaa gagccgtgtt ctttctgtaa ttttaaaaat gcctcagcat ttgttttggc
      961 cgctttaatt tttaatctgt ttctgataat ggcaggatta ttccttgcag actccatctg
     1021 tttatctgta attcttgcaa cttttatagg atcaaattta aagaacactt ttttaaatcc
     1081 ttctctttta tgaagtactg ttttccatga aagcccagcc tgaaatgcat ctagaattat
     1141 atactcaaac catttcctgt catcatgaac aggcacaccc cactcctcgt catgataatg
     1201 tagcattatc tcatcatccc ccatccacga gcatcttggt ttgttgtcag tagtcatgaa
     1261 ttattattaa tattctaacc ttattaatag tacaattctt caattttact ccctcaccta
     1321 ccatgtctta gccttgccac gtgacttagg atcccatcct tgatctaatg ccaattctac
     1381 ttcttgaaac aacttggctt cgaagtcacg ctgtgatctt ggtttctcaa agaaatacat
     1441 attaaacggg aactctacta taagttctct ttctttacca gattcagcat taacacatac
     1501 tgctattcct cgatgatttt cgtaagcatt agacgcccca ccccaatgac gaatcttcca
     1561 aaataattta aagcctttga tagtaaaact gccattatcg atgaattgtg ccattgaaat
     1621 gttcgatgat atattattat taataatctg aaacttatat aatatattta ttgtataaaa
     1681 ataatcttat cacttcaaaa tctccccggt cttctcactc caaagaagaa ggtccagttc
     1741 atctatgttt attcctatat cttttgaaaa gtttatcagt attttctctg tttctaaata
     1801 tttattttct gtagtaggaa tttcgtttga tcttattaga ttaagctcat ataagtaata
     1861 aatgatatgt ttatccaata ttgcatagcc tgagtgtccg gtgtttctta aaaaatggct
     1921 tgcctcttta aaccctatgc cttttatttc cgaagtcgta gccaaaaacc ctcttctctc
     1981 aattgggtct tttatgttag cgatcatatc tctaatattg aaattatatt cagatttcag
     2041 atactctctg cactggatta tatgattagc tctgagattt ggatatctgt aatgcccgat
     2101 taggctagct ctaatttgat taaaatctcc aaaagcaagc acatccttta cagcctctac
     2161 tgagtttaga gccatctttg cgcttgtgcc tgccgcgagt atgcaaaatg tcatctcttc
     2221 aaataacagc tcatctgatg cattttttct gatctgttta aaatcattta gacgagactt
     2281 tataagcggc ttctttttct cgtagctatc ctgcagtctg gaaataagct tgtgattcat
     2341 gatgattgga aaagcttgag gatggtttga ttatttaacg tagattttta aaacgtcgcc
     2401 tctatcgagc ctgtttgact tgagattgtt ccatttctta atcttagaga cagttgtgtt
     2461 gtgctttctt gcaatttccc acaggttgtc gccacttctt acattgtatg atattaggtt
     2521 tgacttactc gaagaagatt ttgcatacac tttagtagga attttgagaa cctgacctac
     2581 tctaatatta gcacctttga tgttattttc tctctttagt gagtggagtc taactccatg
     2641 cctttcagca atttgactaa gagtatcacc acttctcact ttataagttt tggctttctt
     2701 ggcaaccgtt gtggattttg aagaatagct cgatcctccg ggtatagtga gcttctgtcc
     2761 cgggtatata gtatttcctt tgatcttatt tgctgctttt attgatttaa tgctcacccc
     2821 gtgtctgctg gcaatcccgc caacagtgtc accttttctc actctatagg ttgatgactt
     2881 ggatgaagca cgataagagg atgacttact agaactacta tttgatgcat aagaactgcc
     2941 ggtatatttt gtaggaattc ttagtttttg ccctattctt ataagattac ctcttaattt
     3001 attggttctt ttaatggaag aaactgagga cctgtatctt ctggctatac cacccagggt
     3061 atcaccgctt tttacccggt gagtaagata cataggcgca ttatatttct taagggcata
     3121 aagctctgtc tgcttttctc caacaatctg cccatatccg tcaggaacct ttaataaata
     3181 atttgagcca ggcggagtag catttcttat aagactcgga ttaagatctc ttagtgtttt
     3241 gtaatcagtt tttattactc ttgcaatatc ttttaaactt ttttgagcag gaactacagc
     3301 aatttcatat gattgaggtt tgtgataatc aatctctgta aaaccatact tctccggatt
     3361 ttttacaatt actaatgccg ctacaatttt aggtacatac tcttgtgttt cacgaggcaa
     3421 agcctttctt gttcgggaca tctcccaaaa atcgtcatga ttatatttat caattgcacg
     3481 ctgcaccctg tgttctcctg cattgtaacc agctgctgca agctcccaag actcgaacat
     3541 ttcatataga tcgctcaaat aattagcagc tgctattgta gacttttcag gattcatcct
     3601 ctcatctatc caggaattta tagtaagtcc atacttcctg ccggttcctc taataaactg
     3661 ccagggccct acagcaccca ttggagagcg tgcctttaca ttaaaaccgc tttcaatcat
     3721 tgatagataa accaggtctg atggaagatc ctttttcttt aggatctcgg tcatcattgg
     3781 gataaacttg cctgatcttt caagccacct tgtaaaagtc tttctacctc tgttttgata
     3841 gaagtttata tatttctgga ccttatcgtt tagaacaata ggtatttcct gatcggtagt
     3901 tggaagctga tccaccaggt cttcatatat atggtctctg attctaaaaa aatagtagtg
     3961 tgccttccag gtctgatcgt ccggatcgag aaggtaataa cctgttctgt cttccctaac
     4021 aaaatattgt tcggccgaag cttcactctg gcccaataca tggccgccat aataactatc
     4081 actctgattg gataacgcag aaaatgcgtt atcagaaata gactgtgaag cttgcttacc
     4141 tgtagtagca cagcttattg ttaaaagcag aatgggtaga aataaatagt aatacctggt
     4201 gaacattgac gaaaagttaa tcatatttct taacttttgt caaaggtttt tttacatata
     4261 gtatttatat gaaaaaaatt atacaatatg tagtgatatc atatataaga ctaatatctt
     4321 agttaatagt tctacatact tttatacctg gaggaaactg aatggaagta cctgatggtt
     4381 ttgaaacggc actaaacttt ggattttatg attctatctt ttcaagacgc tcacgaagat
     4441 ttggtcttgg aatggaaata gaagaaggaa cgctaaaaca taaatcaagt cacaaaccgg
     4501 tagcactatc aaatctggaa gaagcaatgc tcatatgggc tgggacaggt atgaccggac
     4561 tttcccttgg tgatcttcca agaaccggta tatcatggct gttccactgg acaggaagaa
     4621 gctggccctg ctcatgcaat tctcattcta cagagctctt ttatacaaat gatgacggag
     4681 tatatatggt aaagcttttt gatctgctgc cggacaaaga tcaaacatca atatttcaga
     4741 acaaatcaga tgagcaaaaa ctggaaaggc tcttgtctct ttttaataag agtcagctta
     4801 aacttgagga tggtagagca gtacttccaa agggcgagcc agggcttttt gattttaatg
     4861 catggaacgt aaacaaaccc ggcactacat ttttgatccc tgttacaaac acaacagttg
     4921 agtacatggt actgctattt atttattttg gctcaaagta tggatttaac attatagacg
     4981 agctaaacgg cggcaggagt tgcgggctcg ataagtggat aaaaaaggga tacataaaaa
     5041 atgatgtccg catgtcacta tttgatctag agctaagagt gctaacaaca ctaaatgtag
     5101 aacaggcatt tatatgccaa aatatgaatc tcgcgcttca gtgccttgga cttggcggtt
     5161 gggcttttac ggggcttcta ccccattatg cattgggatt aaaaccagag tttcccggcc
     5221 ttggctttac ttttactaca cccgaagaat ctctacgcag cgaaaagaac cctgttcctg
     5281 taggaaggga tggagtattt gaggcgctct gtcctccgta tgtaaaggat atgggtgaag
     5341 cggtcgataa attcctgcaa attagaggag atttctggaa tgaagacaat ccattccctt
     5401 atcaagaccc tgaaggtatt ttagacgatg agtatcatcc aaaagaagtg agagtagaga
     5461 tagtaaaaga tttttgcagg tatgtgcatg aaaactacgg caggtttccg gcatttttag
     5521 accctatgtt tacaaggctt gttttccagg cacaccacct agatctcgag ttttatgata
     5581 agcactataa ggaaggggct tacacggaac tacataagga tcattttaaa ctatggcatc
     5641 ctgatatggg tgatccgttt ggttaaccgg caatttatta attacttact cttcaccctc
     5701 tcaacataac tgccgtctct tgtatcaact ttaataattt caccaatatc tataaaaaga
     5761 ggtacgttta ttacggctcc tgtttctact gttgcaggtt ttgtagaagc tgaaacggta
     5821 tcacccttaa gaccaggatc ggtctctgta acctcgagat caactgttgt aggaagctct
     5881 ataccaatag gctcgccatt ataatagttt acgatcacat cctgctgttc ttttaagaat
     5941 tttgcgctgt ctcctacctg gtctggtgat attgcaatct gatcataggt ttcgttatcc
     6001 ataaagtgaa aggatatatc atccttatat agatactgca tctgcttttt ttctaaatca
     6061 ggggtttgaa atgtatctcc cgacctgaat tttttttcct gagcagctcc tgtaaagatg
     6121 tttttaagct ttgtagttat aatagcaccg cttcttccca tcttggaatg ctggtaatct
     6181 gtaacttccc atactgaatc ctcaaactct attttaagcc ctttatgaaa cctgcttaca
     6241 tcaactactc ccatcttaaa ttctcctcca aaaagatttt cttattagtg aacctgctca
     6301 tgttcttcta attcttcatt atctattgca tcttctactg gcatactata ctgctcattt
     6361 gcccacgttc caagatcaat catcttgcat ctctctgaac aaaatggcct gtaaatagta
     6421 tcttcccaat tcacagattt attacatgtg ggacacttta ctttcataaa agaagattat
     6481 tgctaaagga tagtaaaaaa tcaatctcaa taaagtggtt agtaaaaaat attatttacc
     6541 actactgcca ctttctgtaa gtgcgttttc aataagctca catattatat gcgctacagt
     6601 gatatgagct tcctggattc tttgggtgga tgtggaagga actgcaatta tgtgatcagt
     6661 gtgtttttta agttttgttg taccgcttcc gctaaaggct acggtcttta gctctttttc
     6721 ttttgcgatc tccaaagcct tcagtatgtt tgctgatttt ccgcttgttg taataccaag
     6781 cgcaatgtca cccttgtttc caatagcctc tatttgtctg gaaaaaatat cattgaacga
     6841 tgtgtcattt gctatagaag taataatgga actgtcagtt gtaagagcta ttgcggcaag
     6901 tgcttttcta tcaatcatga agcggtttac aaactctgcc gctatatgct gcgaatcggc
     6961 agcacttccg ccgtttccaa atattagaac tttctttcct gatttaagcg cacgagttat
     7021 tagctcagaa acctgaacaa tctcgtccag tgattctttt gccatttgca tctttacatc
     7081 tgcgctctct ctaagagcgg atattacatg ttctttcata tcttaagttc ttttaaaaag
     7141 cttttccatc tccccataag taaattcttt tgatacaggt ctaccatgcg ggcagaaatg
     7201 tggcaagtcc gtcttatcaa gctcgtctaa gaggaattgc aacttttcgg tttgcagata
     7261 ctgatttgct gtgacagatt tatggcatgc aatcgttgca attaacaggt caatcctgtc
     7321 actcacactt ttctcggcgt cattttcctt aagctcacct attatatcta caataatctt
     7381 atgccagtca ccatttttta aaaacaccgg cgaacaacgg agcaaatacg accagtcacc
     7441 aaatttttca agatcaaacc ccatctttga aaacagatct atattatcat caacaacagt
     7501 actctccgct cctgtaagct ctattacttc tgggaacaaa aatttttgtg tttgaagggt
     7561 gcttgtctca aattgttttt taaatttttc ataatttacc ctttcatgcg cagcatgctg
     7621 gtcaataaga atcatgccgc tttgtgactc acaaactata taaaggttat ttatctgccc
     7681 tactatatta aggcttccaa aaaacccttt ttgtgtaaaa atatcatcac tttgagattg
     7741 tgcagttaat atttcttcac tgccaacttg cgggctgctc tcaattttat agtcctgttt
     7801 ataatcctgg cctatttccc tgagtaccct taactgatct tcactttgaa agtaatttgg
     7861 acttaccctt cttgtgtctg cgggtctgta gttgtaagta ttattaatat ttttctggtc
     7921 aatattagtg ttactatatg gttttttatc aaatgccatt atccatggag cattttcaag
     7981 catcttgctt attgcgctgc ttataagctc tgagacttta tatgctgaat gaaaacgcac
     8041 ctcacttttt gtaggatgaa catttatatc tacttcggag cttggcaatt gggtaaacag
     8101 cacaccctga gggaacctgc ccttctctat tactttacca aaagatgaaa tcacagacct
     8161 tgttataagt ttatccttta caggccttcc gtttacataa caaaatagtt tatatgtaga
     8221 tgtcctcaca tcgtttgggc tcgataaaaa accgtgcaca acaacccctg aagattcggc
     8281 gcttacctca tgaaggtcta cctttggata aatctctaaa agacgcgaat gcagattaga
     8341 tcttgaactt agatttaaga tggttttatc gttatggatt acttcaaaac tcacgtcata
     8401 tctgataatt gcgtggcgct gtacaatatc taagacattt gaaagctctg tttcaatctt
     8461 ttttaggaat ttaagccttg gaggcgtatt ataaaaaagg cttcttactt caatgagagt
     8521 accctcggga caacccgtat catttacctt ccttattact ccaccatcta taacgatttc
     8581 acaacccgat agttgctggt cctgttttgt aacaagcctg aatttggata tgctgcttat
     8641 actaggaagt gcctcacccc taaatcccag tgtgctgaca tgcagtaaat catcggctgc
     8701 ctttatttta cttgtagcat gacgttcaag acaaagaagc gcatcatccc tgttcatgcc
     8761 tatgccgtca tcctctacac ttataaattt tttgcctccg gatacaagcg tgatctttat
     8821 ctttttagag tttgcatcca gggagttttc aattagctct tttaccactg atgccggtct
     8881 ttctactatt tcaccggcag caattttgga tacaacatca tcaggaagta tagttatgat
     8941 ccccatctaa acccaattct acataagtca aaaattatag tcaaaatgat ataaaaatta
     9001 caaatttcat acaaataaaa aattattaac aattagaaat ttaattatgt ataattgatc
     9061 agttattatt gcttcactaa actacttttt tagatattat cttataataa ctctgaggtg
     9121 ctattttaaa tgaagatgtt caatatatat aaaattggat tactaactat tgctgttttg
     9181 ttgttagcaa cgctaataat accaaccgat gcgaagtcac agcaaaagaa gagaaaaaaa
     9241 gaatctgcca agactgcgct tattattaca gattatgaat ggaaaacggg tggtagaggt
     9301 atagaaggca tcttaaattc ggtaacaatc gaaaatgtag gcagcaaaga ttacgagaac
     9361 atttccatag aagtagagtt aattagcaat aatgatgtgc ctcagggctc acttagagct
     9421 accattaaag acaatctcat ttccggatcg actaagacat ttactaacat tagtcttgga
     9481 ttcatgaata ccgatcttga aaaatcaatt ataaggatta ccggagcgga gcttgttgaa
     9541 agaggtatag attctccaaa agatgtaata ctggtaaaag actggcagtg gcttgaaaca
     9601 aattatggta cagagggaat attaaagtat ataactcttg agaataagag tgatattaac
     9661 tttaagaaaa ttcagataac tattaatttc caagatcctg ctgattcatc aacagtaccc
     9721 tacaggacca taatccatga ttatctgcct gcaaattcag aaaagacgtt caacaatatt
     9781 aacgttggtt ttaaagatag aggagcaaga agtgcagtaa ttacagttag ggatgccact
     9841 aaaataacca aaagagagta caggagcctt cttgcaaagg agggtataag ggttgatgaa
     9901 aatacccctg gtattgatag ttatcccgat tatgacggcc agggagagag agacagaaat
     9961 ctatcacttg ctgaaagata cagaaaatat gtattaaagg ttgatccgga ttccggagag
    10021 caaataattg aagggattga ggatagtgag ggtgaattac aaaaagaaat actgatagaa
    10081 gaacctcaag atacatctcc atattatagg gatagaaatc aatatgccac aagggtggac
    10141 aatgccataa gcgatgaaca gctgctaagg gaacaggcaa agatattccc tgaggttttc
    10201 caggaaattg ttatagtatc tgctgaggct gatacttttg aaactgatac aagaaccgaa
    10261 tcttacagtc agccggtgag tgaacctcaa actcatgaaa gacataagat agagtacagg
    10321 tatgaaaagc ctaaagaaaa gaagaatgtt ataagcagag cattcggatg gagcaaaaat
    10381 cttgtaggtc ttggtgatga tgaagaggaa gaatatgaac ttactgagac caaagtagcc
    10441 gtagataata ctaacacagc ctcaaccaag cgacacccat acacaagtgt agctagggtt
    10501 gatggaaaga ctgtagagta tgaaaatatt agtgaagatg agtatctgat agaaagtgat
    10561 gaagactatg aacctcttcc aaccatagat atagaaatca gggattacaa ggtaaaaccc
    10621 ggaattgtaa gcaccatagg gcagctttca gaaataaccc ttgtaaacag aagtaatatc
    10681 acatacacga acattcagct cgaggtcact ttttataaca gaggtgcaac taaacagctt
    10741 ggttcaaata aatttgtaat aaatgaaatc ctgccgaaac gcacaagaag aacgttcaat
    10801 aatgttgata taggtttttt aaataccgta cccgaagagt tgaagataag aatagtagat
    10861 gctatagata ttaactaaat ctaataccaa attatatatt aaaatcttaa gctaccttat
    10921 acaaacgatt tttctacgca cctatactta tatatagttc atgtgctatt gatatacaga
    10981 ataaatcttg tcagtttgta attccaaatc ctaagattca agaccgacta tttcatcagt
    11041 ctcatctata atttcaccaa gcctgtcctt aaatgtctta attcctccaa gaggaatcac
    11101 aatacaggat cttcccccgc taagctcagt tattctaaga tatttgcctt tatggttttc
    11161 ttttacatca aagaaaaatc ttttggattc aatatcaatg tgtttactta aatgctcttt
    11221 ttctactctt tccataatac caccgcctct tgtcctccct atgagaactg aattcgaaaa
    11281 ttgggcttca ataataacaa atgacttata aaaatcaagg tagagattat acttaaaaaa
    11341 agtatataat aagttgatcc
//
LOCUS       DBNGSV010000134         1055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_579157, whole genome shotgun sequence.
ACCESSION   DBNGSV010000134 DBNGSV010000000
VERSION     DBNGSV010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_579157"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..989)
                     /locus_tag="AC159U_04740"
     CDS             complement(9..989)
                     /locus_tag="AC159U_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3336397.1"
                     /translation="MRINRSDLKLKKNYIQKYRFLISQYEQVKQKKHPQFRYANDFYK
                     FHNTDRRSFLKYYNRYKQSGREADLLPRKRGPKYKTRRALPYVENKVIELREKGAGRY
                     EIHQILKPRLKQYTPSPSGVYNILKRNGLNRLKPAQKASRRKIISNKAGELGHIDAHY
                     LSKDIIQGQHKRLYLVCVIDGCSRVAWAEVVEDIQSLTVMFCVLRCFNIMADEYNIKF
                     ASVLTDNGPEFGTKSSSKKAQHPFERMLMELGVKHRYTRPYRPQTNGKAERFWRTLND
                     DLIEETTFESEEELKQELLEYLVYYNHQRPHQSLGGVPPVEFLKNCPRIT"
BASE COUNT          221 a          249 c          197 g          388 t
ORIGIN      
        1 tgtagatgtc acgtaattcg aggacagttt tttaggaact ctactggtgg tactcccccc
       61 agagactggt gaggtctttg gtgattgtaa tacaccaggt actcaagcag ttcctgtttt
      121 aattcctctt ctgattcaaa cgtggtttct tctatcagat catcgttgag agttctccaa
      181 aacctctcgg ccttgccgtt tgtctgaggc ctgtagggtc ttgtgtacct gtgctttact
      241 ccaagctcca tgagcattct ctcaaacggg tgctgagcct tttttgatga gctctttgtt
      301 ccaaactctg gcccgttatc cgttagtact gaagcaaact ttatgttgta ttcatctgcc
      361 atgatattaa aacacctaag cacgcaaaac atcaccgtga gtgattgtat atcctccacc
      421 acttctgccc aggccactct gctgcacccg tctatcacgc acaccagata caatcttttg
      481 tgttgtccct ggattatgtc cttgctcaga taatgagcgt ctatgtgtcc taactcccct
      541 gctttattgc ttattatctt tctcctgctt gctttttgag cgggttttaa acggttaaga
      601 ccgtttcttt tcagtatatt atacacaccc gagggtgaag gggtatattg tttgagtctg
      661 ggctttagta tctgatgtat ctcgtacctg cctgctccct tctctcttag ctctattacc
      721 ttgttctcta catacggaag cgctcttctt gttttgtatt ttggcccccg ctttcttggc
      781 agcagatccg cttccctgcc gctttgtttg tatctgttgt agtatttaag aaagctcctt
      841 cggtctgtat tatgaaattt ataaaagtca tttgcatacc gaaactgcgg gtgctttttt
      901 tgttttacct gttcgtactg actgattaaa aacctgtact tctgtatata gtttttctta
      961 agttttaaat cactcctgtt tattcgcatc ttaacaaaat accccctcta cttagttact
     1021 tatgtatttt gtctgcgaat tatgtgacat ctaca
//
LOCUS       DBNGSV010000135        10521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1825754, whole genome shotgun sequence.
ACCESSION   DBNGSV010000135 DBNGSV010000000
VERSION     DBNGSV010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1825754"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="AC159U_04745"
     CDS             <1..164
                     /locus_tag="AC159U_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3336398.1"
                     /translation="KENFANPYRAAELGYIDEVILPEDTRSRVIGAFELLEGKRQTNP
                     PKKHGNIPL"
     gene            185..1684
                     /gene="accC"
                     /locus_tag="AC159U_04750"
     CDS             185..1684
                     /gene="accC"
                     /locus_tag="AC159U_04750"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3336399.1"
                     /translation="MFNKILIANRGEIAVRIIRACKELGISTVAVYSEADRKSLHVIL
                     ADEAYHIGPPAPNKSYLVKEKIIEVAKKSGAEAIHPGFGFLSENESFADLCEKEGIVF
                     IGPSSEAIRLMGDKITARKIAQEAKVPLVPGSDGAVTDVQAGVIADKIGYPVMIKASA
                     GGGGKGMRLVRNKEDFESSLRMARSEARGAFGDDSVYVERFVENPRHIEIQILADSHG
                     NVLHLFERECSIQRRHQKVIEEAPSGFISASTRKKMGEVAVRIAKAVNYRGAGTVEFI
                     MDQKQNFYFLEMNTRIQVEHPVTEMITGLDIVKWMVRIADGQKLPFKQSKLQIKGHAL
                     ECRVYAEDPEMNFIPSPGLLHYVKAPSGPGIRDDSCIYSGYEVTSFYDPMLSKLVVWG
                     ETRDDAINKMLSALKEYIVLGVKTNIGFLIRVMRDENFKKVNFDTGFIDRYPELLKPT
                     SEELDLPLIAAGLAIDTMEESANTNKASKPDSNWKTHARQLGVSRNYTL"
     gene            1681..2175
                     /locus_tag="AC159U_04755"
     CDS             1681..2175
                     /locus_tag="AC159U_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR3336400.1"
                     /translation="MTYSFKHDNQIYKLNFENDGGNLTVELEDSKKKVEFKKLDDYLY
                     SIIINGKSHNLAVMKKGKEIQVFYQGNIYNFEAVSELEQARGGAAGGGANQITAPMPS
                     RIVKLLKGEGDAVEEGEGVIVVEAMKMESELKASLSGTVKDIKVAEGDTVEGGAVLVV
                     LSAE"
     gene            2251..3879
                     /locus_tag="AC159U_04760"
     CDS             2251..3879
                     /locus_tag="AC159U_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3336401.1"
                     /translation="MKKLIFFLFLTSFTFLSCGTQETSEDKSAGSQSILRDSLRINIG
                     SEPPTLDWSRSTDSTSYSILINIMEGLTTFGDDYKPVPALAQSWQISEDGKTYTFKLR
                     ENVKWTDGRPLEAKDFEYSWKRLLSPETGADYAYFLYDIENAQEYNTGKIKDKNSIGI
                     KTIDNTTLEVTLKRPASYFLSIVSFMSTFPMREDIIEKYGIKWTEPENMVTLGAYKLQ
                     SWKHHDKLIIVRYDNYWGKKPAIKNVEMIMNENQSSALALYESGELDYVDTKSMPHLE
                     IPRLRLSPDFRTQPQFTVNYLGFNTKKAPFDNPLVRKAFSASINRRNISEVVQGGGIP
                     ATSWIPKGMLAYNEDIGIDFDPDMAKELLKQAGFSNPEDFPKITFLYPDVRNNRIIAE
                     SMQSMWKEYLGIDVELINQEWKVYLDTVTTDPPHIYRAGWAADFPDPHNFMNLFGCSS
                     GNNNTRWCNPEYDKLVESAAGQLDENKRRVLYNKAQIILIETDVPIAPFLLSIQQSML
                     KPYVQGLEPNPLGFIFFNRVSFKDGTPASISSEK"
     gene            complement(3882..4934)
                     /locus_tag="AC159U_04765"
     CDS             complement(3882..4934)
                     /locus_tag="AC159U_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3336402.1"
                     /translation="MPEIKFGLSAPMPGADLDGLLNFSQRADRLGFDTVWFPDHVVFV
                     APTTAYEAWTVGTLAATKTKNINMGTVSDPHRMHPAVMAQRLATIDHISQGRVNLALG
                     VGESMNLDAYNIPWDKPLARVRESIEVMRKLWKEDSHVNYKGKFFQLKDAFLQIKPYQ
                     RNNIPVYLATHTPKGLKLTGETADGWLPIDLNPGLYKEYLGDIKTAAKKAKRSLRKFD
                     PALWVFTSVGENEDEAYKTLEPFKYVLVMQDQLLKAGYDVKIPKKYHGLNYFNVTPTD
                     EKGRQKFRELGQYFPREAILDFTITGSYKDCIKKIERYISKGVRHFVLFYRFSPDPEK
                     ALQTYAKKIIPYFKNK"
     gene            complement(5035..5424)
                     /locus_tag="AC159U_04770"
     CDS             complement(5035..5424)
                     /locus_tag="AC159U_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3336403.1"
                     /translation="MKYLLDTHALLWITSDSSKLPIKCKSLYLEQSNEIYISKASVWE
                     MAIKISLNKLKITNSLEKFVEDHVIANGIKIFDITLEHIYKIESLPFYHRDPFDRLII
                     SQSITEAIPIISTDNSFDMYSVERIWD"
     gene            complement(5421..5648)
                     /locus_tag="AC159U_04775"
     CDS             complement(5421..5648)
                     /locus_tag="AC159U_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016949285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3336404.1"
                     /translation="MTQVNIHEAKTQLSKLIEKIKKGEEVVIAKGNKPVAKLVLIDDL
                     KPKRKLDSAKGLVTISDDFDAPLEDFKEYTH"
     gene            complement(5805..6527)
                     /locus_tag="AC159U_04780"
     CDS             complement(5805..6527)
                     /locus_tag="AC159U_04780"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR3336405.1"
                     /translation="MAFDAKQVEELNNEFSGKAPEDALKWALDNVHPNIALASSFGAE
                     DVVVIDMLMKISPEARVFSLDTGRLNQETYNVMDDVRNKYDLNLEVMFPETAETEAMV
                     REHGMNLFYKGQDNRKLCCGIRKVTPLKKILGTLDGWITGIRADQTQNRGDSAIFEID
                     NVFGGILKVNPIINWSWDETWEYIKANNVPYNKLHDKGYPSIGCEPCTRAIKEGEDLR
                     AGRWWWENESDKECGLHMDHGK"
     gene            complement(6565..7722)
                     /gene="sat"
                     /locus_tag="AC159U_04785"
     CDS             complement(6565..7722)
                     /gene="sat"
                     /locus_tag="AC159U_04785"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR3336406.1"
                     /translation="MSDTIEAHGGTLVNRVLEGSEREQWLGKADSLKSITAPFRVITD
                     LELICNGAMSPLEGFMNKADYESVVENMRLANGLPWSLPIKLPVTTEQAKEFQDGDDL
                     VLKDEAGNILAILHLEEKYEYDKKKEAELVYKTTDEAHPGVAVVYEQGDVLLGGKVSL
                     LNHITGVEFVERRLEPRKTRDYFNKQGWKTVVAFQTRNPIHRAHEYLQKCALEMVDGL
                     LIHPVVGETKSDDIPADIRMRCYEALIENYFPDDRVLLSVLPASMRYAGPREAIFHAL
                     LRKNYGCTHFIVGRDHAGVGNYYGTYDAHHIFREFTLEEMGITPIFFDHSFFCKSCGN
                     MASSKTCGHAKENHVFLSGTKVRDMLTEGVDLPGEFTRPEVSRILMEAYKK"
     gene            complement(7742..8743)
                     /locus_tag="AC159U_04790"
     CDS             complement(7742..8743)
                     /locus_tag="AC159U_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3336407.1"
                     /translation="MELLIPIAIVLSTFLALNIGANNSAASMATAYGAGVRTKREAVT
                     LIAIFAVFGALLAGGPVVETLGKGIVPGDVLTSNIGLVLIVLVIAVFFISWANIAKVP
                     IATTHAIVCAIAGVGLFSKSFNSEKFFEIVIWWVAAPLVMWTINYLLGRYLYFRTLMY
                     LAKRFPEKKINSILTIMLTISGCFLAFSAGANNSANSVGPLVGMGLLTTHNGAILAGV
                     GMAIGAILFGGRVIETVGKQITEICILRAISVEFTGAALLITASFLGIPISMAEIITS
                     GIIGFSCAQHGFAITAQNRHVVQIAFFWFVVPFVAVGISYGLSSLYFKYGTIAMSNF"
     gene            8917..10320
                     /locus_tag="AC159U_04795"
     CDS             8917..10320
                     /locus_tag="AC159U_04795"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HIR3336408.1"
                     /translation="MVKKLSNNKIALISKNKTLHDEIQNLLGAEFDITDFDDRTQGID
                     AGVIFLDVDTVGINHLYGLKDDHFVIVVTAKKGSRYLIEAMTFGAFDCLISPFEKDQL
                     AGTVKRAIGIQQELKGKKIEFPGDIEGSSITCAIVGDSPVLQEVCKTIGQVGRVDVPV
                     LITGESGTGKDLVAESIWKVSRRWEKPFIIINCAAIPDSLLEAELFGHEKGAFTGADT
                     SRKGKFEEADGGTIFLDEIGDMSLTLQAKLLRVLQNQTFQKLGSNKDIEVNVRVIAAT
                     NKNLEQLVEQELFRGDLYYRLNVVEINLPALHERKEDIPLLTQCFARRHSTQAGKDIK
                     GFSTKYIEKLTEHDWPGNIRELENTVRKSIALSKTNYLTSYDLKLPTNIKSNAEDTLS
                     QSQDEIESIVSSKIELSNAGDENLYSETIKEVERSLIEKALAQTKWNRSKAARMLGIN
                     RITLRRKIEEFDISFPN"
     gene            complement(10330..>10521)
                     /locus_tag="AC159U_04800"
     CDS             complement(10330..>10521)
                     /locus_tag="AC159U_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of orotidine monophosphate
                     from 5-phosphoribosyl-1-pyrophosphate and orotate; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3336409.1"
                     /translation="KVVVVEDVVTTAGSVIKAIKAVQELGAIVEAVYVIVDREAGGEE
                     KFDELGIEYKPIFKISDLL"
BASE COUNT         3315 a         2094 c         2030 g         3082 t
ORIGIN      
        1 ataaggaaaa ttttgcaaac ccctacaggg cagcagaact aggatatata gatgaagtga
       61 tcttgccaga agacacaaga tcacgcgtaa ttggagcgtt tgagcttctg gaaggtaaaa
      121 gacagacgaa tccacctaag aaacacggca atattccatt atagagaata aaaaggaaca
      181 acgaatgttt aataaaatat taatagcgaa tagaggtgaa atagctgtaa ggattataag
      241 agcctgtaag gagcttggaa taagcactgt ggcggtttac tctgaagcag acagaaaatc
      301 tcttcatgta atccttgcag atgaggctta tcatatcggc ccgcccgctc cgaacaagag
      361 ctacctggtt aaagaaaaga taatagaagt agcaaagaaa tcaggagctg aagctattca
      421 cccaggcttt ggatttttat cagaaaatga aagttttgcc gatctgtgtg aaaaggaagg
      481 aatagtattt ataggtccaa gctctgaagc aatcagactc atgggtgata agattaccgc
      541 aaggaaaatt gctcaggaag caaaagtacc tcttgttccc gggtcagacg gagcagtaac
      601 agatgttcag gccggagtta tagcagataa aatcggttac cccgtgatga taaaggcttc
      661 agccggtggc ggcggtaaag gaatgagact ggtacggaat aaagaagatt tcgaaagctc
      721 actgcgtatg gcaagaagcg aagcaagggg agcttttgga gatgattcag tatatgtaga
      781 aaggtttgta gaaaatcctc gtcatattga aattcagatc cttgcagact ctcacggaaa
      841 tgtactccat ctttttgagc gtgagtgttc aatacagaga agacaccaga aggtaatcga
      901 agaagctccg tcgggtttta taagcgcttc tacaagaaag aagatgggtg aggtagctgt
      961 aaggattgca aaagccgtta actacagagg agcgggaaca gtagaattta ttatggatca
     1021 gaagcaaaat ttctactttt tggaaatgaa cacccgaatt caggtagagc atcctgtaac
     1081 agagatgata acagggctag atatagtaaa gtggatggtt agaattgcag atggacaaaa
     1141 acttccattc aaacaaagca aattacagat taaaggacac gctctggaat gcagggtata
     1201 tgccgaagac cctgaaatga atttcattcc ttccccaggt ttacttcatt atgtaaaagc
     1261 acccagcggc ccgggaataa gagatgattc atgcatttac agcggctatg aggtaacctc
     1321 attttatgac cctatgctgt caaaactcgt agtatgggga gaaaccagag atgatgctat
     1381 aaacaaaatg ctctcggctc ttaaggaata catagtactt ggcgttaaaa ccaatattgg
     1441 atttttaata agggttatga gggatgagaa ctttaagaag gtaaattttg atacaggttt
     1501 tatcgataga tatcctgaac ttttaaaacc tacaagcgaa gaactagact taccattaat
     1561 tgcagcaggc ctggcaattg atactatgga agaatctgca aacacaaata aagcttcaaa
     1621 acctgactct aactggaaaa cacatgcaag acagcttggt gtatcgagga attatacgct
     1681 atgacatact catttaaaca cgacaatcag atctataaac taaacttcga aaacgatggt
     1741 ggaaacctga ctgtagaact cgaggactct aaaaaaaagg ttgaatttaa aaagctcgac
     1801 gactaccttt attcgataat tattaacggc aaatcacaca atcttgctgt catgaaaaaa
     1861 ggcaaggaaa tccaggtctt ctaccagggt aacatctata attttgaagc tgtatcagaa
     1921 ctcgaacaag ccagaggcgg agcagcaggc ggtggcgcta atcagattac tgctccaatg
     1981 ccaagcagaa tcgttaaact tctaaagggc gaaggtgatg cagtggagga aggtgaagga
     2041 gtaatagttg ttgaagccat gaaaatggag agcgaactaa aagcatctct tagtggcact
     2101 gtaaaagata ttaaagtagc agaaggtgat acggttgaag gtggagccgt tttggtagtt
     2161 ttatctgctg aataattttt tgctatagtt atactcttga gtagtctttt atttatcttc
     2221 taaaaaaact taaataccgg aaggtatcat atgaaaaaat taatattttt tctcttttta
     2281 acctcattta cttttttaag ctgcggcacg caggaaactt cagaagataa atctgccgga
     2341 tcacaatcaa tcttaagaga ctctcttaga ataaatatag gaagtgagcc ccccactctg
     2401 gactggtcaa gatccacaga cagcacatca tattcgatat taatcaatat aatggagggt
     2461 ctaaccacct ttggggatga ttataaacca gtaccagcgc ttgctcaaag ttggcagata
     2521 agtgaagacg gcaaaactta tacttttaaa cttagagaaa atgttaagtg gactgatggc
     2581 agaccgcttg aagccaagga ttttgaatac tcatggaaaa ggctccttag cccggaaaca
     2641 ggtgcggatt atgcctactt tctttatgat atagaaaatg cccaagaata taatactggc
     2701 aagatcaaag ataaaaatag tattggaatt aaaacaattg ataacactac attagaggtc
     2761 acattaaaaa gaccagcatc ttatttcctt agcatcgtaa gcttcatgtc cacttttcca
     2821 atgagagaag atatcattga aaaatatggg atcaagtgga cagagcctga aaacatggtc
     2881 actttgggag catataaact acaaagctgg aagcaccatg acaagcttat aattgttaga
     2941 tatgacaatt attggggcaa aaaacctgca ataaaaaatg ttgaaatgat catgaatgaa
     3001 aatcagtcct cagctcttgc tctttatgag agtggtgagc ttgattatgt agatacaaaa
     3061 agcatgcctc acctggaaat cccgcgccta agactatcac ccgattttcg tacacaacca
     3121 caatttacag tgaattatct gggttttaat acgaaaaaag ccccttttga taaccctctt
     3181 gtaagaaaag cgttctctgc atccataaac aggcgaaata tttctgaagt agtccaggga
     3241 ggaggcattc cggcaacctc atggatacct aaaggaatgc tggcatataa tgaagatatt
     3301 ggtatagatt ttgatccaga tatggcaaaa gaacttctaa agcaggccgg tttttctaac
     3361 cctgaagatt ttcccaagat cacattctta tacccggatg taaggaacaa ccgtataatt
     3421 gcagaatcta tgcaaagtat gtggaaagaa tacttaggca ttgatgtaga gcttataaat
     3481 caggagtgga aagtgtatct tgatacagta accacagacc cacctcatat ttacagagca
     3541 ggttgggcag cagattttcc agaccctcat aactttatga acctgtttgg gtgcagcagc
     3601 ggaaataata acaccagatg gtgcaatcct gaatatgata aacttgtaga aagtgccgcg
     3661 gggcagcttg atgagaataa aagaagggta ctttacaata aggctcagat aatacttatt
     3721 gaaaccgacg taccgatagc cccgtttcta ttatctatac agcaaagcat gttaaagcct
     3781 tacgtgcagg ggcttgagcc aaacccactg gggtttatct tctttaacag ggtttcattt
     3841 aaagatggaa cccctgcatc aatttcatct gaaaaataat tctacttgtt tttaaagtaa
     3901 ggaatgattt tctttgcata agtctgaaga gccttttcag gatcgggact aaatctgtaa
     3961 aacagcacga agtgtcttac tcccttacta atataccttt cgattttctt gatacagtcc
     4021 ttgtatgatc cagtgattgt gaagtcaagt atagcttctc ttggaaaata ctgtccaagc
     4081 tctctgaatt tttgtcttcc tttttcgtca gtaggtgtaa cattaaaata gttaagcccg
     4141 tgatatttct ttggaatctt aacgtcataa ccggccttta gaagctgatc ctgcataaca
     4201 agaacatatt tgaaaggctc cagcgtttta taggcctcat cttcattttc acccactgat
     4261 gtaaataccc acagtgcagg atcaaatttc ctgaggcttc tctttgcttt tttggcagct
     4321 gtttttatat cacccagata ctccttatac agcccgggat ttaaatcaat aggaagccag
     4381 ccatccgctg tttcacctgt aagcttaagt ccttttggag tgtgggttgc cagataaaca
     4441 ggaatgttat ttctttgata aggttttatt tgcaaaaatg catcttttag ctgaaagaat
     4501 ttacccttgt aattaacatg agaatcttct ttccaaagtt ttctcatcac ttcaatcgat
     4561 tctcttaccc ttgcaagagg tttatcccac ggaatgttat aagcgtcgag gttcattgac
     4621 tcaccaacac caagcgcgag attcaccctg ccctgtgata tgtggtcaat agtagcaagt
     4681 ctctgtgcca tcacagcagg atgcattctg tgagggtcag atactgtgcc catatttata
     4741 ttttttgttt tagtagcagc aagagtaccc acagtccatg cttcataagc agttgtggga
     4801 gctacaaata cgacgtggtc aggaaaccag actgtatcaa atcccagcct gtccgctctt
     4861 tgtgaaaaat ttagtagtcc gtcaagatca gcaccaggca ttggtgcaga aagtccaaat
     4921 ttaatttccg gcatattatt tcacctctcc taatagagtt ttaatcatta taaaccattt
     4981 gccccttccg aagcaagggg aaatatttaa gcttagtatt tgtataaatt aattctagtc
     5041 ccaaattctc tctacactat acatatcaaa actattatct gtgcttatga ttggaatggc
     5101 ttcggtaatg gattgagaaa ttatcagtct gtcaaaaggg tctctgtgat agaaaggtaa
     5161 agactctatc ttataaatat gctccaaagt aatatcaaat attttaatac cgtttgcgat
     5221 aacatggtct tctacaaatt tttcaagact gtttgtaatc tttagtttat ttaaactaat
     5281 cttgatcgcc atctcccaca cactggcttt actaatgtat atctcattac tttgctcaag
     5341 gtatagcgat ttacatttta taggaagctt tgaactatca gaagttatcc aaagaagagc
     5401 atgggtatcc aataggtatt tcaatgtgta tattccttaa aatcttcaag tggagcatcg
     5461 aaatcatcag atatagttac caaacctttg gcagaatcta attttctttt gggttttaga
     5521 tcatctatca atacaagctt ggcaacaggc ttattacctt ttgctataac cacttcttca
     5581 cccttcttta ttttttctat tagcttggaa agttgagtct ttgcttcatg aatattaact
     5641 tgtgtcatat atgaacctct ttagtattgt atacaatata cttagctaag ttatatgact
     5701 aagtcaactt tgtcaaatct atctttggga tagtattaaa aaaaagggca accaaacggt
     5761 tgcccttttt aattctggat agttaatttt gagtttgaaa ggaattactt accgtgatcc
     5821 atatgaagtc cgcactcttt gtcagactca ttttcccacc accagcggcc ggctctcaga
     5881 tcttcgcctt ccttaatggc tcttgtgcat ggctcacagc ctatgcttgg gtagccttta
     5941 tcgtgtagtt tgttgtatgg aacattgttt gctttaatat attcccatgt ctcgtcccat
     6001 gaccagttga taattgggtt cacttttaaa ataccgccaa atacattgtc gatttcgaag
     6061 attgcagaat cgcctctgtt ctgagtctgg tctgctctga ttcctgtaat ccatccatct
     6121 agggttccaa gaattttctt aagcggtgtt acttttctta ttccacagca aagttttcta
     6181 ttatcctgac ctttatagaa cagattcatt ccatgttctc ttaccatggc ttcagtttct
     6241 gcggtctctg gaaacataac ctcaagatta agatcatatt tatttcttac gtcatccata
     6301 acgttatatg tttcctgatt aagccttcct gtatcgagtg aaaatactct ggcttctggg
     6361 cttattttca taagcatgtc tataactaca acgtcctctg ctccaaagct tgaagctaga
     6421 gctatatttg gatgaacatt atcgagcgcc cattttagtg catcctctgg ggccttacca
     6481 ctaaattcgt tgttgagttc ttcaacctgt tttgcatcaa atgccatgac tgcctcctac
     6541 aaatgtatat ttatttgttt ttatttattt tttatatgct tccattaaaa tcctggatac
     6601 ttcaggtctt gtaaattccc ccggcagatc cacgccttct gtaagcatat cccttacctt
     6661 tgtaccgctt aaaaatacat gattttcctt tgcatgaccg catgttttag agcttgccat
     6721 gttaccacag ctcttacaga aaaatgaatg atcgaagaaa atcggggtta ttcccatctc
     6781 ttctaatgta aactctctga atatatgatg agcatcatat gtgccgtaat aatttcctac
     6841 tcctgcatga tcccttccta caataaagtg tgtacatcca tagttctttc ttaaaagagc
     6901 atgaaaaatc gcctctcttg gcccggcata cctcattgaa gccggcagca ctgaaagtaa
     6961 taccctgtca tcagggaaat agttttcaat aagtgcctcg tagcacctca ttctgatatc
     7021 agccggaata tcatcagatt ttgtctcacc aactacagga tgaattaata atccatcaac
     7081 catctcgagt gcacattttt gcagatactc atgtgccctg tgtatcgggt tcctggtctg
     7141 aaaagccact actgttttcc agccctgttt attaaaatag tctcttgttt ttctaggctc
     7201 taatcttctt tcaacaaact ccactcctgt aatatgattg agaagactaa ccttgccgcc
     7261 aagcagcaca tccccctgct cataaactac tgctactcca gggtgggctt cgtctgtggt
     7321 tttataaaca agctccgcct cttttttctt atcatattca tacttttctt caaggtgaag
     7381 tattgctaat atgtttcctg cctcatcttt taaaactaaa tcatcgccat cctgaaactc
     7441 ttttgcctgc tcggttgtaa caggaagctt gattggcaaa ctccagggaa gcccatttgc
     7501 aagcctcata ttttctacaa cgctttcgta atcagccttg ttcataaacc cttcaagtgg
     7561 actcatggct ccattgcata taagctctaa atctgtaatt accctaaaag gtgctgtgat
     7621 acttttaaga ctatctgcct taccaagcca ctgctctctt tctgatcctt ccaacaccct
     7681 gtttacgagt gttccgccat gtgcttcaat tgtatcgctc atttacttaa attctccttg
     7741 tctaaaaatt cgacattgct atagttccgt acttaaagta tagagaggaa aggccgtaac
     7801 tgattcccac tgctacaaac ggtactacaa accaaaaaaa tgctatctga actacgtgcc
     7861 tgttttgggc cgtaattgca aacccatgct gtgcacagga aaagcctatt ataccagaag
     7921 ttatgatttc cgccatagat attggaattc ctaaaaaaga tgccgttata agcaatgctg
     7981 ctccggtaaa ttcaacagaa atggccctta atatacaaat ttccgtaatc tgctttccaa
     8041 ctgtttctat taccctgccg ccaaaaagaa ttgctcctat tgccatacca accccggcaa
     8101 gaattgcgcc gttatgagta gtaagaagcc ccatgcctac gagtggtcct acggagtttg
     8161 cagagttgtt ggcacctgca gaaaatgcca aaaaacaacc tgatatcgta agcattatag
     8221 taagaatgct gtttattttc ttttccggaa atcttttagc cagatacatg agagttctaa
     8281 aatagaggta tcttcctaaa agataattaa tagtccacat cacaagtggg gcagccaccc
     8341 accatatgac gatttcaaaa aacttctcag agttaaatga tttagaaaaa agtccaacac
     8401 ctgcaatagc acatacaatt gcatgagtag tagcaatagg aaccttggcg atattagccc
     8461 atgaaatgaa aaatactgct atcacaagca ctatcaatac aagcccaata ttagatgtaa
     8521 gcacatctcc gggcacaatc cccttaccca gggtttctac aactggccct ccggcaagta
     8581 acgccccgaa cacagcaaaa atagctatta atgttacagc ctctcttttt gtgcgcaccc
     8641 ctgcgccgta agctgttgcc atagatgcag ccgagttgtt ggctcctata ttcagggcca
     8701 aaaacgtaga taaaacaata gcaatcggta ttaatagttc caaaaacctt tcaccaataa
     8761 attagatcaa ttatgcagta attattaaaa tgacaaaatt agaagctata tagcaagttt
     8821 tattccaaat tttgtgcaat aatttcagca tgttacataa tattatcaat cttacagttg
     8881 aatacctgtt atccaatatg tatatttttg atacatatgg ttaaaaagtt atcaaataat
     8941 aaaattgctt taatttcaaa gaacaaaacg cttcatgatg aaatccaaaa cctccttggc
     9001 gctgaattcg atatcactga ttttgatgac agaactcagg gcatagatgc cggtgtaatt
     9061 tttctcgatg ttgacacggt aggaataaac catctgtacg gattaaaaga tgaccacttt
     9121 gtaattgttg tcacggccaa aaaaggctcg agatatttga ttgaagccat gacatttggg
     9181 gcatttgatt gtttaatcag cccatttgaa aaagaccagc ttgcaggcac cgtaaaaaga
     9241 gcaattggca tacagcagga gcttaaagga aagaaaatag agtttccagg agatattgaa
     9301 ggcagcagca taacctgtgc tattgtaggt gattcacccg tgcttcagga agtgtgtaaa
     9361 accatcggac aagtgggcag agtagatgtg ccggttctca ttacaggtga gagcggaaca
     9421 ggaaaggacc tcgttgctga atctatatgg aaagtaagca gaagatggga gaaacctttt
     9481 atcattataa actgtgcagc tattcctgat tcgcttttgg aagctgagct ctttggacat
     9541 gaaaagggag cttttaccgg tgctgatacg agcaggaagg gaaaattcga agaagcagac
     9601 ggcgggacta tatttctgga cgagattgga gatatgtctc taactcttca ggcaaagctt
     9661 ctgagggtgc ttcaaaacca gacatttcag aaacttggca gtaataagga tatagaggtg
     9721 aatgtaagag taatagccgc tacaaataaa aatttggagc agcttgttga gcaggaactt
     9781 tttcgcgggg atttgtacta taggttaaat gtagttgaaa ttaatctgcc tgccctgcat
     9841 gagagaaaag aagacattcc gcttcttact cagtgctttg cccggcgaca cagtacacag
     9901 gctggaaaag acattaaagg attttccact aaatatatag aaaaactaac tgagcatgac
     9961 tggccaggga acataaggga gctagaaaat actgtacgaa aatcaattgc actctctaag
    10021 actaattatc tcacttccta tgatcttaaa ctacctacaa atattaaatc taacgctgaa
    10081 gatacattat cccaaagcca ggatgaaatt gagtctatag tttcttcaaa gattgaatta
    10141 tcaaatgcag gcgatgaaaa tctatatagt gaaactataa aggaagttga aaggtctctt
    10201 attgaaaagg ctttggccca gacaaagtgg aacagatcaa aagccgcacg tatgcttggg
    10261 ataaacagga ttacacttag aagaaagatt gaggagtttg atatcagttt cccaaactag
    10321 tactcctggt tacaataaat cactaatttt aaagatgggt ttgtactcta tgccaagttc
    10381 atcaaacttc tcctcaccac cagcttctct gtctactatt acataaaccg cctcgacaat
    10441 tgcgccaagc tcctgaactg cttttatggc ttttattact gacccagcgg ttgttacaac
    10501 atcttcgact acaactacct t
//
LOCUS       DBNGSV010000136         7283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_926768, whole genome shotgun sequence.
ACCESSION   DBNGSV010000136 DBNGSV010000000
VERSION     DBNGSV010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_926768"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="AC159U_04805"
     CDS             <1..1095
                     /locus_tag="AC159U_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR3336410.1"
                     /translation="INSGAFVDLGGIEGFIPFSEITWGRIKNPNEVLSIGQEVSTKVI
                     NIADGPKITLSLKQTNLDPWENVAQKYKSGNNVKGKVVSVKDFGVFVELEPGIEGLIH
                     SSEITWTKKFRHPKEIVDEGSVVEAVVLDVDTEKKRISLSLRQIEPSPWEIFKDSNPP
                     GKKVKGTIRNITANGLFVEVAEGLVGLVRPDQISWQGKEDPNQNYKTGQEIEVSVINI
                     DVNNQRVALGIKQLTTDPWIEANNSYKARESVVTAKVTGVNDYGISVELENNIDGFIR
                     MAEYAQTGSRDLVKQVKVGDEITCLVMGFDKRKRQVNLSKKAYESFQEKQQVNNFISS
                     QGDSNVTLGDLFGKEFKAPESEQNSEQGTD"
     gene            1110..1985
                     /gene="sppA"
                     /locus_tag="AC159U_04810"
     CDS             1110..1985
                     /gene="sppA"
                     /locus_tag="AC159U_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3336411.1"
                     /translation="MKKILYLFFIFFLLAVAFMMGLGAGFIFDDDDGFKAGDKVALLS
                     VEDVILDSKVYLESITSIKKDKDIKALVLRINSPGGAVGPSQEIYSEILKLREKIPVI
                     ASLGAVAASGGYYIACGADKILTNPGTVTGSIGVIAQFVNYKELLEWAKVDVEVLKSG
                     KFKDSGSPLKDLTPEERAYLQSTIDDVHNQFKKAVGDTRKLKPEEVNAIADGRIFTGQ
                     QAVDLKLVDEIGTMNDAIDAAAKLGGIEGEPNVTHYPKKKTRLVDVLSNIESNVIPKI
                     PFNSRFGLFYLVNTI"
     gene            2006..2485
                     /locus_tag="AC159U_04815"
     CDS             2006..2485
                     /locus_tag="AC159U_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3336412.1"
                     /translation="MKVLWAPWRIEYITKEKQSGCVFCDTPAEQNDKNNLILYRGNTC
                     FIILNRYPYTNGHLMAVPYKHTNELNDLSSEERAELLDLTIKCKNILGIMNPNGFNIG
                     MNLGKVAGAGIDDHLHFHVVPRWDGDTNFMPVLGSVKIMPEYLDQTYDKLKEQLESN"
     gene            2497..2874
                     /locus_tag="AC159U_04820"
     CDS             2497..2874
                     /locus_tag="AC159U_04820"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HIR3336413.1"
                     /translation="MRSIKILIQALIFVALIVVIIQNIDVFTYEFELGLDLEVWSSGT
                     FIIKNIVLIASSFLLGVILTIFWGAFSSASKSSTIKQQKKKIKELESSKPETASTYSA
                     EETVSSEAHEVPSASNDPFKSPS"
     gene            2898..3836
                     /gene="lipA"
                     /locus_tag="AC159U_04825"
     CDS             2898..3836
                     /gene="lipA"
                     /locus_tag="AC159U_04825"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3336414.1"
                     /translation="MDSSLQSEKVRKPSWIKAKIPGGDNYKKLKSLMKENNLHTVCEE
                     AKCPNIGECWAAGTLTFMILGDTCTRSCGFCHVKTGKGPELDWEEPKRVAQTVEKLIS
                     NNANITHVVITSVNRDDKNLESAQIFGETISRVKQHNPNVKIEVLIPDFKGESEALEQ
                     VLIAKPHVLNHNIETVPRLYNHPQKTSSGRTRSVRPQARYEWSLELLKRSKQYGNDGM
                     LTKSGIMVGLSEEMDEIIDTLKDLKEVGCDIVTIGQYLQPTIDHLPVTKFYHPSEFES
                     LKQYGESVIGIPHIESGPLVRSSYHAEKQVIKLNQI"
     gene            3925..4509
                     /locus_tag="AC159U_04830"
     CDS             3925..4509
                     /locus_tag="AC159U_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336415.1"
                     /translation="MKSLLTSLLILFVTVVCINSAESKELKYKNTTFTTTVGNVRLQA
                     ELADQGKENSDKVSVTVPAVIFDKPIVIEDVRKVTKSQGKEIDFLVAYHKANIEGDKE
                     KILSFWHPAEKEEVSKDVNDPELLKHTKEYMTKNPGMTIHGIIKQKSSISVITGTDFV
                     FGISIRPKDGKLFLTNKPDNDLELAIIEGSFMKF"
     gene            4840..5412
                     /locus_tag="AC159U_04835"
     CDS             4840..5412
                     /locus_tag="AC159U_04835"
                     /inference="COORDINATES: protein motif:HMM:NF023580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR3336416.1"
                     /translation="MTIHRSGVVRKRHKGIEYLGGGYDEVFYPKITYSYSVNGVLYWG
                     GSDIYYDTFSHREGFSHGKAWDIARKYKPESVVSGYYNPKDPEESFLEVRSSSFMGMA
                     ILFFTFPLTIAIIIIRNEPDSDEVHKKELFMYSIPPLALFFIYQVYHLSLGGEMDGIY
                     WLTWLFMLLPAYILYCALKPSDDNVEQLGS"
     gene            5518..6426
                     /locus_tag="AC159U_04840"
     CDS             5518..6426
                     /locus_tag="AC159U_04840"
                     /inference="COORDINATES: protein motif:HMM:NF023048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HIR3336417.1"
                     /translation="MNNKYRVTILTCFIVFLSLIIFGLPTNSFAGAKACSGDNMCAKG
                     EICGYGQCIPGCRVSTECRPGQYCINNECTDVICKPGSSRQCYEAGDETKSVGMCNSG
                     IQFCLEDGKSYSLCTGQVLPQIEICDMQDNDCDGQIDNGVDCECKPGSKRTCYNGQQG
                     TLDVGECKAGIQYCEMDNSWGKCLEVVNPSNEICDGKDNDCNGIIDDNDKCECPPAAI
                     RWCYTHDPTTADVGECREGRQVCKADYTWASVCLGEIGPVKEIMADGKDNDCDGKVDN
                     VFADPNEMILDKPVVIEPEPPLEIIE"
     gene            6511..6684
                     /locus_tag="AC159U_04845"
     CDS             6511..6684
                     /locus_tag="AC159U_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336418.1"
                     /translation="MKKCPFCAEYIQDDAIKCRFCNELIDKTPKPKTKWYHTNTGVIF
                     ALLAIGPFALPLV"
     gene            complement(6892..7122)
                     /locus_tag="AC159U_04850"
     CDS             complement(6892..7122)
                     /locus_tag="AC159U_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336419.1"
                     /translation="MPQRILFAIAMKINFGKNSPNDDSSKVRNSNNKGNSRTLEIEFV
                     IALLAEGALSILSKLDIPAEKFNIPKPIVIRK"
BASE COUNT         2479 a         1231 c         1522 g         2051 t
ORIGIN      
        1 atcaactccg gagcatttgt tgaccttggt ggaatagaag gatttatacc atttagtgag
       61 attacatggg gaagaattaa aaacccaaat gaagtattga gtattgggca ggaagtttcc
      121 acaaaggtta taaatattgc agatggtcca aagatcacac tgagcctaaa gcagaccaat
      181 ttagatccat gggaaaatgt ggctcagaaa tacaagtccg gcaacaatgt taaaggaaaa
      241 gtggtttcag taaaggattt tggagtattt gttgagcttg agcccggaat cgaagggctt
      301 attcatagtt cagagattac atggacaaag aagttcagac atcctaagga aatagttgac
      361 gaaggtagtg ttgtagaagc agttgtgctc gatgtagata cagagaagaa aaggataagc
      421 cttagcttaa gacagataga gccaagcccc tgggaaatat ttaaagatag caacccgccc
      481 ggcaagaaag taaaaggtac aattagaaac ataactgcta atgggctttt tgttgaagta
      541 gccgaaggcc tggttggact tgttaggccc gatcagattt catggcaggg aaaagaggat
      601 ccaaatcaaa attataaaac cgggcaggaa attgaagtat ccgttataaa tatagatgta
      661 aataatcaaa gggttgctct tggtataaaa cagcttacga ctgatccttg gatagaggca
      721 aacaacagct ataaggcaag ggaaagtgtt gttaccgcaa aagtaacagg agtcaacgat
      781 tatggaatat ccgttgaact cgaaaataat attgatggtt ttattagaat ggctgaatat
      841 gcacaaacag gttcgagaga tttagtaaaa caggttaagg ttggtgatga gatcacatgc
      901 cttgtaatgg gatttgataa gagaaagagg caggttaacc tgagcaaaaa agcatacgaa
      961 tcctttcagg aaaagcagca ggtcaataat ttcatatcat ctcaaggcga ttctaatgta
     1021 acacttggag atctgtttgg taaagagttt aaggcacccg aatcagaaca aaactctgaa
     1081 cagggaaccg attagcaaac tacacataaa tgaaaaaaat actttatctg ttttttatct
     1141 tttttctgtt ggctgtcgcg tttatgatgg gcttgggtgc gggtttcatt tttgatgatg
     1201 atgatggctt taaggctgga gacaaagttg cgcttctaag tgttgaagac gtaatcctgg
     1261 attcaaaggt ttacctggaa agcataacga gtataaaaaa agataaggat attaaagctc
     1321 ttgttctgag aatcaattct ccaggtggtg cggttggtcc ttcacaggaa atttacagtg
     1381 aaatactaaa gctaagagaa aaaatccctg taattgcaag tcttggggca gtagcagcat
     1441 cagggggtta ttatattgca tgtggtgcag ataaaatatt aacaaatcca ggaacagtaa
     1501 ccgggagcat cggcgttata gcccagtttg taaactataa agaactcctt gagtgggcta
     1561 aggttgatgt tgaggtgtta aagagtggaa agttcaaaga ctccgggtct ccccttaaag
     1621 acttaacccc agaagaaagg gcatatctac aatcaactat tgatgatgtt cacaatcaat
     1681 tcaaaaaagc tgtaggagac acgcgcaaat taaaaccaga agaggtaaat gctattgctg
     1741 atgggagaat ctttacaggt cagcaggctg tcgatcttaa gcttgtagat gagattggaa
     1801 caatgaatga tgccatcgat gccgcagcca agctaggtgg tatagaaggt gaacctaatg
     1861 taacccacta ccctaaaaag aaaacgagac ttgttgatgt actgtcaaat attgaatcaa
     1921 acgttattcc gaaaatccct tttaacagcc gctttggctt attctattta gtaaatacaa
     1981 tttaagttta gactaaatta aaactatgaa agtactctgg gctccctgga gaattgaata
     2041 tattaccaag gaaaaacagt ctggctgcgt gttctgtgat acacccgcag aacaaaatga
     2101 taagaataat cttatactct accgtggaaa tacatgtttt attattttaa accgctatcc
     2161 ttatacaaat ggacacctga tggcagttcc atataagcac acaaacgagc ttaacgactt
     2221 aagctctgag gaaagagcag aacttttgga tttaacgatt aaatgtaaaa atattcttgg
     2281 tataatgaat ccaaacggat ttaacattgg tatgaattta ggtaaagtag caggagcggg
     2341 tattgatgac catcttcact ttcatgttgt gccaaggtgg gacggtgata caaactttat
     2401 gccggtatta ggaagcgtaa agataatgcc cgaatatttg gatcaaacat atgataaact
     2461 taaagaacaa ttagaatcta actaaaggag aatgatatga gatctataaa aattcttatt
     2521 caagccttaa tatttgtagc attaatagta gttattatac aaaacataga tgttttcacc
     2581 tacgagttcg agttgggtct tgatttagaa gtttggagtt ctggaacttt tattattaaa
     2641 aacattgttt taattgcatc atcttttctt ttaggagtca tactcacaat tttttgggga
     2701 gcattcagca gtgcatcaaa gagctcaaca attaagcaac aaaagaaaaa gataaaagaa
     2761 ctggaaagct caaagccaga aactgcatcg acttattcag ctgaagaaac agtatcatct
     2821 gaagcacatg aagtaccatc tgcatctaat gaccctttta agagcccaag ttaatcccgc
     2881 ataaatctaa attatatatg gatagttccc ttcaatcaga gaaagtaaga aaaccatcct
     2941 ggataaaagc aaagatcccg ggtggggata actataaaaa actgaaatct cttatgaaag
     3001 aaaacaacct ccacactgtg tgtgaggagg cgaagtgtcc caatatagga gagtgctggg
     3061 cagccgggac actgacattc atgattcttg gagatacatg tacacgcagt tgtggctttt
     3121 gtcatgtaaa gacaggcaaa ggccccgagc ttgactggga agaaccaaaa agagtcgcac
     3181 agacagtcga aaagttaatt tctaataatg caaacataac ccatgtggtg ataacctctg
     3241 taaaccgcga tgataaaaat ctggagagtg cacagatttt tggtgaaacc atttctagag
     3301 taaagcaaca taatcctaat gttaaaatag aagttttaat acccgatttt aaaggtgagt
     3361 ctgaagcatt agaacaagta ctaattgcaa aaccccatgt gctaaaccac aatattgaaa
     3421 cagttccaag actctacaac catcctcaga aaacttcatc tggaagaacg aggtcagtgc
     3481 gccctcaggc aagatatgag tggtcacttg agctgcttaa gagaagcaag cagtatggca
     3541 atgacggaat gcttacaaaa tcaggcatca tggttggtct tagtgaagag atggatgaga
     3601 taatagatac tctcaaagat ttaaaagaag taggttgtga tatagttaca atcggccagt
     3661 acttacaacc tacaatagac catcttcccg tcacaaaatt ttaccaccca tcagaattcg
     3721 agtctctaaa acagtacggc gaatcagtta tcggaatccc tcacatcgaa tccggccctc
     3781 tagtgagaag ctcctaccat gcagaaaaac aggttataaa actaaaccag atttaggata
     3841 tctaattcct aaatttcttt taacctctat tatattcgtg ttatattcaa ttgatacatc
     3901 acattgtgat aaggaatgat tctgatgaaa agcttactta cttcactact aattttattc
     3961 gtcacagttg tatgtataaa cagtgcagaa tctaaagaac taaaatataa aaacacaact
     4021 tttacaacca cagtcggcaa tgtaagactc caggcagaac ttgcagacca gggaaaagaa
     4081 aatagtgata aggtatctgt aactgttcct gccgtaattt ttgataaacc gattgttatt
     4141 gaagacgtaa gaaaagttac aaagagccaa ggtaaggaaa ttgattttct agttgcctac
     4201 cataaggcta atattgaagg agacaaggaa aaaatattat ccttttggca ccctgcagaa
     4261 aaagaagaag tcagcaaaga tgtaaatgac cctgaactac ttaagcatac gaaagaatat
     4321 atgaccaaga atcctggcat gaccattcat ggaatcataa agcaaaagag cagcatatca
     4381 gtgattacag gaacggattt tgtttttggt atcagcataa gaccaaaaga cggaaagctg
     4441 tttttaacaa ataagccgga caatgacctg gagcttgcta ttattgaagg atcgtttatg
     4501 aagttttagc attcagtaaa tttccaaaaa ttcaaatcat cctaatttcc tacaattcag
     4561 aatagccatc tctattcact actgccagtc tggtacacct tcgaaaatcg atatattatc
     4621 atatcaatta atttgatctt gtgataatta ctaatcgtta agtcatacac acagcttaat
     4681 atatagacca tgccaaaact agatgaatta gttagaaaaa ttaatttcag atatgtggga
     4741 tttgtttttt ttcttacaat aagcctgtta tcaggaaaac gtttatttag tgagatttat
     4801 acggggggta aaagagcgct tacataagaa cctgtggaaa tgactattca cagatcaggc
     4861 gttgttagaa agaggcataa agggattgag tatcttggag gcggttatga tgaagtgttt
     4921 tatccaaaga ttacatattc atatagtgta aatggagttc tgtactgggg tggtagtgat
     4981 atttactatg acactttttc tcacagggag ggatttagcc atggtaaggc atgggatatt
     5041 gcaagaaaat ataaacctga gtccgttgtg agcggatatt ataatcccaa agatcctgaa
     5101 gaatcatttc ttgaagtaag atcatcatca ttcatgggaa tggctatatt attttttaca
     5161 ttccctctaa ctattgcgat cattattata agaaacgagc ctgattctga tgaagtacac
     5221 aaaaaggaac tgtttatgta cagcataccc ccgcttgctc tattttttat ttaccaggtt
     5281 taccatcttt cactaggtgg agagatggac ggcatatact ggttaacgtg gttatttatg
     5341 ctactgcccg catatatatt atactgcgcc cttaagccat ctgatgataa tgtggaacag
     5401 cttggatcat gattagctta tctgcttaca taacgccctt catccttgat ttggctgtga
     5461 gttattatat attttataaa taagtcttat cgaatagagc ataatttgaa aatcggaatg
     5521 aataacaaat acagagtaac catactaacc tgcttcatag tatttttaag ccttatcatt
     5581 tttggtttac cgactaactc atttgcaggt gctaaagcat gttcaggtga taacatgtgt
     5641 gctaagggtg agatatgtgg ctacggacaa tgtattccag gttgcagagt aagtacagag
     5701 tgcagacctg gacagtattg tataaataat gaatgtacag atgtaatatg caaacctgga
     5761 tcatcacgcc agtgttatga agctggagat gaaaccaaat ctgtaggtat gtgtaattca
     5821 ggaatacaat tctgtctgga agatggaaag agttattcat tatgtacagg ccaggtttta
     5881 ccacaaatcg aaatatgtga catgcaggat aatgactgtg atggacaaat cgataacggt
     5941 gttgattgtg agtgtaagcc gggatcaaaa agaacatgtt ataacggaca acaagggacg
     6001 cttgatgtcg gtgaatgcaa ggctggcata cagtactgtg aaatggataa ctcttggggt
     6061 aagtgccttg aagtagtaaa tccaagcaat gaaatatgtg atggaaaaga caatgactgt
     6121 aacggcataa tagacgacaa cgataaatgc gaatgtcctc cggcagcaat tagatggtgc
     6181 tacactcatg accccactac agcagatgtt ggcgagtgcc gtgagggaag acaggtttgt
     6241 aaggcagact acacatgggc ttcggtctgt ttaggagaaa taggtcccgt aaaagaaatt
     6301 atggccgatg gtaaagacaa tgactgcgac ggaaaggttg ataatgtatt tgcagacccc
     6361 aatgaaatga ttcttgataa accagtagtt attgagcccg aacctccgct ggaaattatt
     6421 gagtagtaag gattttcaat ttcataattc ttcctatcaa caattctaac tttgcaacat
     6481 aagtttaatt ttatatgtta agctacctgt atgaaaaaat gccctttttg tgccgaatat
     6541 atccaggatg atgctattaa gtgccgcttt tgcaatgagc taattgacaa aactcccaag
     6601 cctaaaacaa agtggtacca tactaacacc ggagtaattt ttgccctgct tgccataggc
     6661 ccatttgccc tgccacttgt atgaatcaat cccgaatata aaacacacat caaaatcctt
     6721 cttacaatac ttatcctggg tgggagctta tggttttatt ttgctactaa ggatatttat
     6781 gaattgttgt gaaataaata attatgtcat tatttcgtaa ataacctgta ggagtttctg
     6841 cataacctgg aaaatgaaaa gggtcctgat aattattggt ttatcggcag ttcacttcct
     6901 aataactatg ggctttggta tattaaattt ttctgcaggt atatccaatt ttgataatat
     6961 agacaaagcc ccctcagcaa gtaatgcgat tacaaactct atttctaatg ttctggaatt
     7021 ccctttattg ttactgttcc ggactttaga gctatcatcg ttcggagagt tcttgccaaa
     7081 gtttatcttc attgcaattg caaatagtat tctttggggc actgcgatat atataattta
     7141 taaatatgtt atcaagaaac cagaaaagac ccggcagacg tgacctgcat ttcttgaata
     7201 gggacgggat ggtcgcatgc aaccctcgtg accacgaagc tgcgcataga gctgaggttg
     7261 aggggattgc tactgagaat aaa
//
LOCUS       DBNGSV010000137         5654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_996251, whole genome shotgun sequence.
ACCESSION   DBNGSV010000137 DBNGSV010000000
VERSION     DBNGSV010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_996251"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="AC159U_04855"
     CDS             complement(<1..1034)
                     /locus_tag="AC159U_04855"
                     /inference="COORDINATES: protein motif:HMM:NF051235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3336420.1"
                     /translation="MNRLSKKAILYLILIIVPFLYISINGLIAYSETKNKIVLIKING
                     MINPASSDYIRVGLQKAVETNANALVIEMDTPGGLLNSTKDIVKMILNAEVPVVVYIH
                     PSGSSATSAGVFITLSAHVAAMNEGTSIGAAHPVSIGQGGKEKKKQRDEDSDEDAEKK
                     KDIMEEKIENYAASFIESIAEKRGRNVEWAIEAVRNSASITSTEALEKNVIDLISPSL
                     NDLLSKIDGRKVKVNETEVILATKDASIQTFEMSAKQKLVDVLSTPDIALLLISLGSL
                     GLLLEFYNPGLIFPGVAGAICLMLGFVSFQIIPFNYGGVVLLLIAVGLFVAEMYVPSF
                     GLLTTGGVIS"
     gene            1136..1774
                     /locus_tag="AC159U_04860"
     CDS             1136..1774
                     /locus_tag="AC159U_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336421.1"
                     /translation="MANKVKVTQKELKKPDKFLEFIDRSTNYLAESYKLIVYFVGAII
                     ALILIFFLINSYQNKNTSKANIIYKEGITAKTSGDFDTALTKFGILQKDYSGQKVSDL
                     SLYYTGSIYYNREDYDNSLTYVSKFLNTGPKDPNMIDAAYSLISLSYLNKGEYQLAID
                     FSNRISNPDSPYINNAQMTKGLALEKLGKHDEATEIYNKILTTMYPGTFQPQ"
     gene            1845..2204
                     /locus_tag="AC159U_04865"
     CDS             1845..2204
                     /locus_tag="AC159U_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3336422.1"
                     /translation="MEIFGAIILGLFAGGASGLFGIGGGLVMIPALVFFFQLTQHQAQ
                     GTSLAVLTLPVVFLGALKYYHEGNVQLKLAAFIAVSFVIGAYGGSILVHKISSPMLKK
                     MFGGLLFVVSIRMILGK"
     gene            complement(2209..3138)
                     /gene="miaA"
                     /locus_tag="AC159U_04870"
     CDS             complement(2209..3138)
                     /gene="miaA"
                     /locus_tag="AC159U_04870"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3336423.1"
                     /translation="MTRDLPKIVVILGPTSSGKSDLACELSKHFNFEIINADSLQIYK
                     YLDIGTAKPDLKILDSVPHHLISIIKPDEQFNAGIYREKANEVIDILHNRNKKILIVG
                     GTYLYVRVLIEGLIEGVEPDTSFRDELKALRAAYGTEHIYNNLKDIDPESALSINEND
                     YVRMERALEVYHLTGQKMSDMQKEHGFSKKKYNVFKIGIDIERELLKKIIGRRLDNMM
                     DRGFTSEVKSLRERGYDKSLKPMQSIGYKEINDYLDGVMSLEDAREKIIINTRRLAKR
                     QMTWLRRDKEINWYKIPQDFKHILEDLNTFYEN"
     gene            complement(3149..5254)
                     /gene="pnp"
                     /locus_tag="AC159U_04875"
     CDS             complement(3149..5254)
                     /gene="pnp"
                     /locus_tag="AC159U_04875"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3336424.1"
                     /translation="MSSGHRTISEELFERTVEIETGRLAKQANGAVLFRYADTVVLAT
                     VAAGKEAIEGEDFLPLTVNYQEKSYAAGRIPGGYFKREGRPTEKEILTSRLIDRPIRP
                     LFPKKYTFPTQVIVTVMSTDQQNDPDVLSITAASSALMVSNIPFAGPVAGVRVGRIDG
                     QFVCNPTKTQLDDSDIDLVVAGTEDAIVMVESGSKEVSEEEMIDALMYAHDCIRKFIE
                     PQKKLISDIDTAKIELPEEEDNSALHNEISEFSKDNLKEAILTKEKQQRASNVNKVYD
                     DTIAHFTEKYPETDVLVTKQFDTLKKNVLRQMVVDDKKRIDGRSFTDIRDISIETGFL
                     PRTHGSALFTRGETQAAVIATLGTSYDEQKIDSLEGDTKKSFILHYNFPPFSVGEVGN
                     RLGPGRREIGHGALAERAIKYMVPNKDDFPYTIRIVSDILESNGSSSMATVCGTSLSL
                     MDAGVPIKDHVAGVAMGLIKEEDKVAILTDILGDEDHLGDMDFKVAGTEKGITALQMD
                     IKITGVTREILETALTQAKDARMFILGKMKEAITGPRESISDYAPMITSIKVDPAKIK
                     DIIGSGGKTIKKIVEESGAQIDIQDDGTVNVAAVVRADSDKAIKMIEMLTEEIEVGQL
                     YIGIAKRILDFGAVVELASGKDGLVHISELAPERVKEVTDIINEGDEVLVKCIEKGRD
                     GKIRLSRKAALEENIEDYR"
     gene            complement(5275..5544)
                     /gene="rpsO"
                     /locus_tag="AC159U_04880"
     CDS             complement(5275..5544)
                     /gene="rpsO"
                     /locus_tag="AC159U_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033016867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3336425.1"
                     /translation="MDVTVMEKSEVIKNFALHSTDVGSPEVRIAILTKRIKNLARHLQ
                     KSKKDVHSRKGLIDMINKRRKLLRYLNMKDSSRYIQLVEKLELRK"
BASE COUNT         1597 a         1156 c          874 g         2027 t
ORIGIN      
        1 aaacttatta cccccccagt agttagcagc ccaaaactag gaacatacat ttcggctaca
       61 aacaacccta ctgcaattag tagcagtaca acccccccat aattaaatgg tattatctgg
      121 aaagatacga aaccaagcat gagacatatg gctcctgcaa ctcccggaaa tatcagtcca
      181 gggttataaa attcaagaag caatccaagt gagccaagag atattaaaag aagtgcaata
      241 tcaggggtgc taagtacatc taccagtttt tgtttggcgc tcatttcaaa agtttgtata
      301 gatgcatcct tagtagctaa aatcacttct gtttcgttta cttttacttt tctaccatcg
      361 attttcgata gcagatcatt tagtgatgga gagatcaggt ctattacatt tttttctaat
      421 gcctctgtag atgtgatcga tgcactgttt cttacagctt caattgccca ctctacattt
      481 ctgcctctct tttctgcaat actttcaata aatgatgcag cataattctc tattttttct
      541 tccattatgt cttttttctt ttctgcatct tcatcactat cttcatccct ttgctttttc
      601 ttttcttttc caccctgtcc tatactcaca ggatgggcag ctccaatact tgtaccttca
      661 ttcattgccg ccacatgagc cgaaagagtg atgaaaactc cagcagatgt agctgaagaa
      721 ccgcttggat gtatatatac tacaacagga acttcggcat ttaggatcat ctttactata
      781 tcttttgtag aattgagaag ccctcccgga gtatccattt ctattacaag agcattagcg
      841 ttagtctcta cggccttttg cagcccaact cttatataat cggaactagc gggattgatc
      901 attccattga ttttgataag tactatttta ttctttgttt cggaataagc gataagtcca
      961 tttatggata tatatagaaa cggtactata atgagaatta aatataatat agctttcttt
     1021 gacagtctgt tcactaaaat attttagctt tcataaatca aactgcaagt aaatgacata
     1081 gagtatttaa ttcttatcga cttgtctttt tagttactct tattataatt ccgccatggc
     1141 aaataaagta aaagtaactc aaaaagaact aaaaaaacct gataagtttt tagaattcat
     1201 tgacagatct acaaactatc tggctgaatc ttataaatta attgtatatt tcgtgggagc
     1261 tattatcgct cttatattaa tattctttct tattaacagc tatcaaaaca aaaatacatc
     1321 taaagctaat attatttata aagaaggaat tacagcaaaa acctctgggg attttgatac
     1381 agcccttacc aaattcggca ttcttcaaaa agattattca ggtcaaaaag tatcggattt
     1441 gtcgctctac tatacaggtt ctatctatta taatcgcgaa gattatgaca actctcttac
     1501 atatgtaagc aaatttttaa ataccggacc taaagacccc aatatgatag atgctgcata
     1561 ttcacttatt tccttatcat atttaaataa aggtgaatat caattagcaa tagatttttc
     1621 aaatagaatc agtaatccag acagcccata tattaataat gcgcaaatga caaaaggtct
     1681 ggcacttgaa aaacttggaa agcatgatga agcaaccgag atttataata aaattctaac
     1741 aactatgtat ccaggcacat ttcagccgca atagatacaa catctactag ctgtcttgtt
     1801 atttgattct aagtataata agtccaaaat acctggagga aattatggaa atttttggtg
     1861 cgattatttt aggtcttttt gcaggagggg ctagcggtct atttggtatt ggtggtgggc
     1921 tggtaatgat tccggctctt gtattttttt tccagcttac ccaacaccag gcccagggta
     1981 catctctcgc cgtccttact ctgcctgtag tcttcctggg agctctaaaa tattaccatg
     2041 aaggcaacgt gcagttaaaa ctggcagctt ttatagccgt tagttttgtg ataggagctt
     2101 atggtggatc gatacttgta cacaagatat cctcacccat gcttaagaaa atgtttggtg
     2161 gtctattatt tgtagtatca ataagaatga tactgggaaa ataaagattt aattttcata
     2221 aaacgtgttc aagtcctcta ggatatgttt aaagtcttga ggaatcttat accagtttat
     2281 ttctttatct cttctaagcc atgtcatctg tctttttgca agccttcttg tatttataat
     2341 aatcttttct ctagcatctt ctaaagacat aactccatcc agataatcat ttatttcttt
     2401 atagccaata gactgcatgg gttttagcga tttatcatat cccctttctc ttaggctttt
     2461 aacttcagaa gtaaacccac gatccatcat attatcgagt cttctcccaa taatcttttt
     2521 aagtaattca cgctcgatat ctattcctat tttgaataca ttatatttct tttttgaaaa
     2581 tccgtgttct ttttgcatgt ctgacatttt ctgtccagtc agatgataaa cctcaagagc
     2641 tctttccata cggacataat cattttcatt aatactcagt gctgattcgg gatctatatc
     2701 ttttagatta ttataaatgt gttcagttcc atatgcagct cttagtgcct ttagttcatc
     2761 tctaaagcta gtatcaggct ccactccctc aattaacccc tcgataagca ctcttacata
     2821 aaggtaagtt ccgccaacta tcaatatctt tttatttcta ttatggagaa tatctataac
     2881 ctcatttgct ttttcacggt atattccggc attaaattgt tcatcaggct ttataatact
     2941 gattaaatga tgaggaactg aatctagtat ttttaaatca ggcttggctg tacctatatc
     3001 gaggtactta taaatctgga gggaatccgc gtttataatt tcaaaattaa aatgctttga
     3061 gagttcacat gcaagatctg atttaccact tgaagttggt cctaaaataa caactatttt
     3121 tggtaaatct ctagtcaatt tacagtaatt atctgtaatc ttctatattt tcttcaagag
     3181 cagcttttct gcttaacctt attttgccgt ctctgccctt ttcaatacat ttaacaagta
     3241 cttcatcacc ctcgtttata atgtctgtaa cctcttttac tctctcaggt gcaagctccg
     3301 atatatgtac tagtccatcc tttccgcttg caagctcaac aactgcgcca aaatcaagta
     3361 tacgttttgc tatacctatg tatagttgcc caacttctat ctcttcagtc aacatttcaa
     3421 tcatctttat tgctttatca gaatctgccc ttactactgc tgctacattc accgtaccat
     3481 catcctgaat atcaatttga gctcctgatt cttctactat tttctttatg gtcttaccgc
     3541 cgcttcctat aatgtcctta atctttgcag ggtcgacttt aatagatgtt atcataggtg
     3601 cataatcaga tatagattct cttggccccg tgatagcttc tttcattttt cccaatatga
     3661 acattctggc gtcttttgcc tgagtaagtg cagtctctaa aatttctctt gttacacctg
     3721 taattttaat atccatctgt agcgcagtga tacctttttc tgtaccggct actttaaagt
     3781 ccatatcccc tagatggtct tcgtcaccta gaatatcagt taaaatggca accttatctt
     3841 cttcttttat aagacccatt gcaacacctg caacatgatc ctttatgggt actccggcat
     3901 ccataagtga gagtgaagta ccgcagacag tggccatgga ggatgatcca ttggattcca
     3961 gaatatctga tacaattctt atcgtatatg gaaaatcgtc tttatttggg accatatact
     4021 ttattgctct ttctgcaaga gcgccgtgtc ctatttctct tctaccaggt ccaagtctgt
     4081 taccaacctc accaactgaa aatgggggga aattgtagtg taatatgaag ctttttttcg
     4141 tatctccctc cagtgaatca attttttgct cgtcatatga agtacctaga gtagctatta
     4201 cggctgcctg tgtctctcca cgtgtaaaaa gagctgatcc atgcgttctt ggtaaaaacc
     4261 ctgtttcaat cgaaatgtct ctaatatcag taaatgatct gccgtctatt ctctttttat
     4321 catcaactac catttgcctt aaaacattct tttttaatgt atcgaattgt tttgtaacaa
     4381 gaacatcagt ctctggatat ttttcagtaa aatgggcaat agtatcatca tataccttat
     4441 taacattact ggctctttgc tgtttttctt ttgtgagaat tgcttctttt agattgtctt
     4501 ttgaaaattc acttatttca ttgtgaagtg ctgaattatc ctcttcttca ggaagttcta
     4561 ttttagcggt atctatatcg gaaataagct ttttttgtgg ttctataaac tttctaatac
     4621 aatcatgtgc atacattagt gcatctatca tctcttcttc ggatacttct tttgatccgc
     4681 tttctaccat tactattgca tcttcagtgc cggctacaac aagatcgata tctgaatcat
     4741 ctaattgagt ttttgtagga ttgcatacga actgtccatc aattctacca acccttactc
     4801 ctgcaacagg tcctgcaaaa ggaatgtttg ataccataag agctgatgaa gctgcggtta
     4861 tagataatac atcggggtcg ttttgctgat cggtggacat taccgtaacg attacctgtg
     4921 taggaaaagt atatttttta gggaacaggg gtcttattgg tctatcaata agtcttgaag
     4981 taagaatttc tttttctgta ggtcttccct ctcttttaaa atatcccccc gggattcttc
     5041 cggctgcata ggatttttcc tgatagttta ctgttagtgg taaaaaatct tctccttcaa
     5101 tcgcttcctt tccagcggct actgttgcca gtaccacagt gtcggcgtat ctaaacaaaa
     5161 ctgctccgtt tgcctgcttt gcaagccttc cagtttcaat ctcaactgtc ctttcgaata
     5221 actcctcaga tatcgtacga tgaccagaac tcaaattagt aattcctccg tgttttattt
     5281 tctcagctca agtttttcaa ctaattgaat gtagcgactg ctgtctttca tatttaaata
     5341 cctaagaagc ttccttctct tgtttatcat atcaattagt ccctttcttg agtgtacatc
     5401 cttcttggat ttttgaaggt gtcttgcaag atttttaatt ctctttgtaa gtattgcgat
     5461 tcttacttca ggagaaccaa catctgtaga gtgaagtgca aagtttttta taacttcact
     5521 tttttccatt actgttacgt ccatctaaat ccctccaaat attttatcta attaaacact
     5581 cttaatattt taataatttc ttcagaatca tccatcataa ccaaatcctc aagtgataca
     5641 agtgatttag ctat
//
LOCUS       DBNGSV010000138         3431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1341451, whole genome shotgun sequence.
ACCESSION   DBNGSV010000138 DBNGSV010000000
VERSION     DBNGSV010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1341451"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1342)
                     /locus_tag="AC159U_04885"
     CDS             complement(128..1342)
                     /locus_tag="AC159U_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3336426.1"
                     /translation="MLIIENSKTTLKRFHEFKPKSIRYKNDDDYAEHYRELLDKSVAC
                     RMRCSTPVSIMLSGGMDSNSIVPFAVKHNPHKKKTRAISWTFNKLQQCDEREYIDKAI
                     EMYDLIKIQFNADDLWPLKNKDKFFNNPGTPMENPYRELKENIYSLCIENGSRLIFNG
                     WYSDRFYAGSDLWLTDLIKDKKISRFINDLVWLIGEEGLFNLRSSQPARNLFSFLKFL
                     KPKRNPTDNYSWLTDFSKSQIHETLIDKTTYQYFSNPKRIQNMLSAIGFFGQSSEHFH
                     LSNHSIELECPYRDIRLVEFMMNIPSYQLYNKGTKKYIAKNAMKNLLPDKILNRNSPT
                     LLTPLFNLGMQDKEVEWIESILSSSDEWDKYIKRGEVLNSLRNKNQPGRKELIVWQCI
                     SFINWTNSHSYN"
     gene            complement(1382..1960)
                     /locus_tag="AC159U_04890"
     CDS             complement(1382..1960)
                     /locus_tag="AC159U_04890"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR3336427.1"
                     /translation="MSAFLAVYDRSGKKLGKKHLNAMMKKLNHRGPDGSDSVYLENVF
                     LSHHHLWITPQEVGEKQPLAYSSNNLFICFDGRLDNRDKIIDLIYSGNIGLEEKSDAA
                     LLLLLYEKLGDKCFDKLIGPYSITIYDKAKSRVICARDPLAERGFFYHISNNTFIACS
                     EEAPILYYPSVTKTLNEKTIAHFFAMEAPRDG"
     gene            complement(1986..3284)
                     /locus_tag="AC159U_04895"
     CDS             complement(1986..3284)
                     /locus_tag="AC159U_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR3336428.1"
                     /translation="MANDGKLVYNDSGEAFEVQNLNSSDLVVYSKSGSSVSKHTNLDI
                     TDNGGSYSVKFAGDKNNDIQYHVFEHGAVLTPELEVAPDTSDLKTGQAQYLIISHPAF
                     LNDLSDLITEREKQYSVKAVDVEAVYTEFNGGIFGPEAIRSYIDYAVNNLGTVHVLLV
                     GGDSYDYHDYIGNSSLSFIPTFYGKVHEFANFAPSDALIADIDGDNVQDVSIGRFPVR
                     TSQELANIIEKTLNYPLIANNNAVFAADADDGSISFSSDSNNFIDQLPIGWSVDKAYL
                     EGSTVVEAKATLIDSINDGRALTSFFGHSSFGVWTFSGLFNLTDAQNLTNVGRPTVVT
                     QYGCWNTYFVDPALNTLGHKFLLSGLNGAAAVTGPTSLTASSSDRVFGDIFLQNLFTG
                     QTIGEAIRDTKQQIAPHGPSRNDVIIGWNLLGDPALVITE"
BASE COUNT         1012 a          657 c          638 g         1124 t
ORIGIN      
        1 ggcacttcca atacaaacat ttgtattagc ttgcttatct tactatttga tagttttgta
       61 ttcttaagta acttcttttg catagtttca gcttagtata ctttgctgtc ctatgcgagc
      121 tccaaaatta attgtaacta tgactattgg tccagtttat aaaacttata cactgccaaa
      181 caataagctc ttttcttccc ggttggtttt tgtttctaag cgaatttaaa acttcacccc
      241 tttttatata tttatcccac tcatcgcttg aagataagat gctttctatc cattcaacct
      301 ctttatcctg cattcctaaa ttaaatagag gagtaagaag cgtagggctg tttcgattta
      361 agattttgtc tggcagtaaa tttttcatgg cattcttggc tatgtatttt tttgtacctt
      421 tattatatag ctggtaagaa ggtatattca tcatgaattc aacaagtctt atgtcccggt
      481 agggacattc gagttctata gaatgattgc taaggtggaa atgttcagaa gattgaccga
      541 aaaatccaat agccgaaagc atattttgta ttcttttagg atttgaaaag tattgataag
      601 ttgttttatc aataagagtt tcatgaattt gagatttaga gaaatctgta agccagctgt
      661 aattatctgt cggatttctt tttggtttta aaaatttcag aaaagaaaac aagtttctag
      721 ccggctgaga tgaacgtaag ttaaacagtc cctcttcacc tattaaccac accagatcat
      781 ttataaaacg agatatcttc ttatctttta taagatcagt gagccaaaga tcagagcctg
      841 cgtaaaatcg gtcagaatac cagccgttaa aaatgagcct gctgccgttt tcaatgcata
      901 aactgtagat attttcttta agctctcggt aagggttttc cataggtgtg ccggggttat
      961 taaagaactt gtctttattt ttaagcggcc ataggtcatc tgcattgaac tgtatcttta
     1021 ttaaatcgta catctcgatt gccttatcta tatattctct ttcatcgcat tgctgtaatt
     1081 tattaaatgt ccatgatatg gcccttgttt tctttttatg aggattgtgc tttacagcaa
     1141 aaggtactat agaattcgaa tccatgcctc cgctaagcat aattgaaaca ggagttgagc
     1201 agcgcattct gcaggctacg cttttatcca agagttctct gtagtgctca gcataatcat
     1261 catcattttt atatcggata cttttaggct taaactcatg aaaccttttt aatgtagttt
     1321 tactattttc aattattaac aaattaccgg ggagtagttc ttttatatct ctaaaaaatg
     1381 tttatccatc ccggggtgct tccatagcaa agaaatgagc gatagttttc tcatttaatg
     1441 tttttgtaac agagggataa taaagaatag gagcttcctc tgagcaggca ataaaagtgt
     1501 tgttacttat atgataaaag aaccctcttt ccgctagagg atcacgagcg cagattactc
     1561 tgctttttgc cttatcgtat attgtaatgg agtaagggcc tatgagtttg tcaaaacatt
     1621 tatcccctaa cttttcgtac aataatagca atagagcagc atcagatttc tcttctaatc
     1681 ctatgttgcc ggaataaatc aggtctatta tcttatccct gttatcaagc cttccatcaa
     1741 agcatataaa caggttgttg gagctataag ctaatggctg tttctctcca acctcttggg
     1801 gagtaatcca taaatggtgg tgggaaagga aaacattttc aagataaact gaatcagaac
     1861 catcggggcc acgatgattc agttttttca tcatggcatt taggtgtttt ttgcctagtt
     1921 tcttaccgct tctatcgtaa actgccagaa atgcactcaa agtgcataaa atataccact
     1981 aaatcttatt cagtgataac aagcgcaggg tctccaagaa ggttccaacc gattatcacg
     2041 tcgtttctcg aaggtccatg aggagcaatc tgctgctttg tgtctctaat tgcctcacct
     2101 atggtttgtc ctgtaaacag gttttgcagg aatatatcac cgaatactct atcagatgat
     2161 gaagctgtaa gactcgtggg tcctgtaaca gcagcagcac catttaagcc actgaggaga
     2221 aatttgtgtc ccagggtgtt taatgcaggg tctacaaaat atgtgttcca acagccgtat
     2281 tgagttacaa ctgtaggcct tccaacattt gtaagatttt gggcatcggt gagattaaat
     2341 agacctgaaa aagtccatac accaaatgaa gaatgtccaa aaaagcttgt aagggcgcgt
     2401 ccgtcattta tagaatcaat aagagtagcc ttagcttcta ctactgtgga accttctaaa
     2461 taagctttat caactgacca tccaattggt agctggtcta taaaattatt actatcactt
     2521 gaaaacgata tagatccgtc atctgcatca gcagcaaaaa ctgcattgtt attagctatg
     2581 agcgggtagt taagtgtttt ttcaataatg ttggcaagct cctgtgaagt tctcacaggg
     2641 aacctcccga tagatacatc ctgtacatta tctccatcta tatcagcgat caaggcatcc
     2701 gaaggggcaa agttggcaaa ttcgtgtacc tttccataaa acgtaggaat aaagctaagt
     2761 gagctgtttc ctatataatc atgatagtca taagagtctc cgccaacaag tagcacatga
     2821 actgtgccaa gattgtttac agcgtaatct atgtaacttc tgatggcttc gggcccgaat
     2881 atgcctccgt taaactctgt atatacagcc tctacatcta ctgctttcac gctgtactgt
     2941 ttttcccttt cagtgataag atcactgaga tcgtttagga atgcaggatg tgaaatgatg
     3001 agatactgtg cctgtccagt tttaagatcg gaagtgtccg gagctacttc tagctcaggt
     3061 gttaaaactg caccgtgttc aaaaacatgg tactgaatgt cattgttctt gtctccagca
     3121 aatttaactg agtagcttcc gccgttgtca gttatatcaa ggttagtgtg tttagaaacg
     3181 gatgaaccgg atttactgta tacaacaaga tcagatgaat ttagattctg tacctcaaat
     3241 gcctctccac tatcgttata aacaagtttt ccatcattag ctatgaagct tctttcatac
     3301 ccaactgacc ttccccccta taatagtacc agattacaag ttagaatttt aggtaataaa
     3361 ccctatgaat aggaggcaag ggaaatgccc aggaagcgtt acacgccaga gcaaataata
     3421 agatatttaa g
//
LOCUS       DBNGSV010000139         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_372017, whole genome shotgun sequence.
ACCESSION   DBNGSV010000139 DBNGSV010000000
VERSION     DBNGSV010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_372017"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..508
                     /locus_tag="AC159U_04900"
     CDS             158..508
                     /locus_tag="AC159U_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336429.1"
                     /translation="MFSQLPDYDRQQLRSWSKQKIYLQIEYLKNLGDEFGFELIVMAM
                     PIKKGSPHQYDWLKDIKEKGVKYKDFNKNDIWTEKKNDLFFKNDYHLTVRGNRVLAEM
                     LYDHLTNPKHLSVY"
     gene            591..668
                     /locus_tag="AC159U_04905"
     tRNA            591..668
                     /locus_tag="AC159U_04905"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:627..629,aa:Glu,seq:ctc)
     gene            728..1363
                     /gene="trhA"
                     /locus_tag="AC159U_04910"
     CDS             728..1363
                     /gene="trhA"
                     /locus_tag="AC159U_04910"
                     /inference="COORDINATES: protein motif:HMM:NF011669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR3336430.1"
                     /translation="MIERSQTLREEIANSIAHGVFLLFAIIGSPFLIAKSIEAKDNLV
                     VITAAIFAAAVIFVYLVSTIYHAMPKNRAKEILRLVDYGAVYLLIAGTYTPIALNVIG
                     GGAGMALFAAEWILAITGIIFLYYTGLKHKGVLVLFYLIMGWLILFAIKPLWFSLPVW
                     GIFWIFAGGLAYTLGIPFYRLSHLNFRHFIWHIFVVAGTACHSVAVFKYAV"
     gene            complement(1432..1797)
                     /locus_tag="AC159U_04915"
     CDS             complement(1432..1797)
                     /locus_tag="AC159U_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor family protein"
                     /protein_id="HIR3336431.1"
                     /translation="MSKFGVTNKKEEELYLKMENLSIFEDDIEEKFIKSSGKGGQNVN
                     KNSTCVQLKHLPSGIIVKYQKERTQNLNRFFARRALIEKLDKQINGAKSSEILKREKL
                     IKQKQKRKKRAKKKLEKID"
     gene            complement(1882..2094)
                     /locus_tag="AC159U_04920"
     CDS             complement(1882..2094)
                     /locus_tag="AC159U_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336432.1"
                     /translation="MIIKVAVAIAFVIAFAYIAFWYKDTTEEAVQTTGKYIKTGIRAT
                     EHSREMIKGSKESMKEREKMADFDDQ"
BASE COUNT          627 a          376 c          391 g          796 t
ORIGIN      
        1 ccggcacttc caatacaaac atttgtatta gcttgcttat cttactattt gatagttttg
       61 tattcttaag taacttcttt tgcatagttt cagcttagta tactttgctg tcctatgcga
      121 gctccaattt ttaatatcct ttttatccta caaagacatg ttttcccagc tgccggatta
      181 tgatagacaa cagcttagaa gttggtctaa acaaaagatt tatctgcaaa tagaatatct
      241 aaaaaatctg ggtgatgaat ttggttttga attgattgtg atggcaatgc ctattaaaaa
      301 aggttctccc catcagtatg actggttaaa agacataaag gaaaaaggag ttaaatacaa
      361 agatttcaat aagaatgata tttggacaga gaaaaagaac gatctgtttt ttaaaaatga
      421 ttaccacctt accgtacggg ggaatagagt gcttgctgaa atgctttatg atcatttaac
      481 aaatcctaag catctgtccg tatattaata taataattcc tttagtttta tttgttatat
      541 ttttgaattg ttgagaatct ttattataag gtatattaat ttttctcaga ggccccatcg
      601 tctagcctgg cccaggacgc agccctctca aggctgaaac acgggttcga atcccgttgg
      661 ggctaccact aattaattat cccctatcat tcgtattaca attggtataa aatattgttg
      721 aaccgtaatg atagaaagaa gccagacctt aagggaagag attgctaata gtattgctca
      781 cggggtgttt ttattatttg ctattattgg cagtcctttt ttgatagcaa aatcgataga
      841 ggcaaaagat aacctggttg taattactgc cgctattttt gcagcggctg taatatttgt
      901 ttatctggtt tccaccatct accatgcaat gccaaaaaac agggccaagg aaatactgcg
      961 ccttgtggac tacggagccg tataccttct gatagcgggt acatatactc caattgcgct
     1021 taatgtaata ggagggggag ccggcatggc gctttttgca gctgaatgga ttcttgcaat
     1081 aacaggaata atttttttat actatactgg gttaaaacac aaaggagttc tggtcctgtt
     1141 ctatttaatt atgggctggc ttatattatt tgctataaaa cccttatggt ttagtcttcc
     1201 tgtctggggg attttctgga tttttgcagg cggattggcc tacactctgg ggataccttt
     1261 ttacagatta agtcacctaa acttccgaca ttttatctgg cacatttttg tagtagccgg
     1321 aaccgcatgt cactcagtag cagtatttaa gtatgctgtt tgaaaaagac tagttaagtt
     1381 aataatattc gtacattaaa tcaattttga aatgaaggct cttaattcag actaatcaat
     1441 tttttctagc tttttctttg cacgtttttt ccttttttgt ttttgtttaa taagtttttc
     1501 gcgttttaga atttctgaag acttggctcc gtttatttgc ttatccagtt tttctataag
     1561 cgcccttctt gcaaaaaatc tgtttaggtt ttgggttctc tctttttggt attttactat
     1621 tatgcctgaa ggaagatgct ttagctgaac acaggtagaa ttcttgttta cgttttgccc
     1681 gcccttacca gatgacttaa tgaacttctc ctcaatgtca tcttcaaaga tagacaggtt
     1741 ttccatcttt aaatacaact cttcttcctt tttatttgta actccaaatt tgctcattgt
     1801 ataaataaat tatacgtggg atataaaaga tgtgttaatg atgtgttaat agtgaaatta
     1861 aggaacttag aatgtaaaaa attactggtc gtcaaagtcg gccatttttt ctctttcttt
     1921 catactttct ttacttccct ttatcatctc acgggaatgc tcggtagccc taatgccggt
     1981 ttttatatat ttgccggtag tttggacagc ttcttctgta gtatctttgt accaaaaagc
     2041 aatatatgca aatgctatta caaaggctat ggcgacggct acttttataa tcatatttta
     2101 attataaaat gattaaatta aatattcaat aggataatgt ttaacctact ggaagattaa
     2161 taatgaattt tgtaccctga ctcatttcac
//
LOCUS       DBNGSV010000140        10069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1826333, whole genome shotgun sequence.
ACCESSION   DBNGSV010000140 DBNGSV010000000
VERSION     DBNGSV010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1826333"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..356
                     /locus_tag="AC159U_04925"
     CDS             168..356
                     /locus_tag="AC159U_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336433.1"
                     /translation="MENLKKKYHSKYPFTSDGVYVYFGEIQNMPGHCVVCDFKSGQIY
                     TGYHIENFIEISEEGYDS"
     gene            398..610
                     /locus_tag="AC159U_04930"
     CDS             398..610
                     /locus_tag="AC159U_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3336434.1"
                     /translation="MATQKEILKEAIKLKPTEKAKLIDQLILSLDKPDSDLDKLWAEE
                     AESRLEAYKRGELVAVSLKEVVAKYL"
     gene            607..903
                     /locus_tag="AC159U_04935"
     CDS             607..903
                     /locus_tag="AC159U_04935"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3336435.1"
                     /translation="MKVKFLVEAQLELDETINYYNQEVAGLGDSFLREILSTIDRIIE
                     FPNSWHLLSNEIRRCQTRRFPYGLIYSIIDNELLVLSVSNLHRRPNHWRDRIKE"
     gene            908..1285
                     /locus_tag="AC159U_04940"
     CDS             908..1285
                     /locus_tag="AC159U_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR3336436.1"
                     /translation="MKFCTVINCMDGRVQLPVIKYLQERFGAEYVDSITEPGPNRILA
                     EQTNTALLDSISTRLKISLGNHESIGIAVAGHHGCAGNPTPKDEQTQHTLNAVKFLKS
                     KYPGVEIIGLWVDGNWEVEEIQL"
     gene            1314..1535
                     /locus_tag="AC159U_04945"
     CDS             1314..1535
                     /locus_tag="AC159U_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336437.1"
                     /translation="MLTKLLEQAFNKASKLPKEEQDILAKAILDELEAEQKWEALFEK
                     SGDLLEELAKEALEEHKAGKTKSLNPDQL"
     gene            1532..1810
                     /locus_tag="AC159U_04950"
     CDS             1532..1810
                     /locus_tag="AC159U_04950"
                     /inference="COORDINATES: protein motif:HMM:NF048436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR3336438.1"
                     /translation="MKSFTTDKFRSLFAKLPKSIKQRARESYRLFKNNPHHPSLHFKT
                     VHLTEPIYSVRISKDYRAVGIINDEKIFWFWIGPHSEYDQLLKNYQKN"
     gene            1819..2592
                     /locus_tag="AC159U_04955"
     CDS             1819..2592
                     /locus_tag="AC159U_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3336439.1"
                     /translation="MSTIQINKNDIHYLSNTKIDGSHPTLFLLHGAGQSHLTWEYQLD
                     YLQALDGYNFVIPDLPGHGKSDGEGLKSVEEYTEFIKELISELDLKEIIFIGHSMGGA
                     VAQLLALEEPDYLKAIILVCTGASMSVARETLLAVRDNYEVFCEVAPTRAFAESSPEI
                     LKTKFKEGLQNTKQKVAYDDLLACNVFDISDKISGINIPTLIIAGSEDILTIPKRSEY
                     LHHQIFDSELHIVEGSGHFVMQEKTEEFNALVFDFIESL"
     gene            complement(2599..3366)
                     /gene="cofE"
                     /locus_tag="AC159U_04960"
     CDS             complement(2599..3366)
                     /gene="cofE"
                     /locus_tag="AC159U_04960"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR3336440.1"
                     /translation="MSSKLELIPVTGIPEIKEGDNLARIIANRLKSSDIELAEKDILV
                     IAQKIISKSEGKVVDLNDITPTPFAEKLGDELLKDPRMVEVILGETKRIVKMQRKTDT
                     GVMVVENKDGLVLANAGIDASNVPGNDKVTLLPDDSDGSAKKIAKDVTNESGKNVAVI
                     ITDTVGRPWREGLTEIAIGTWGINPLKDYRNQKDKEGYELTATLIAIGDEIASAAGLL
                     MGKNDAVPVVIVRGYDYEFEDVGSKSLIRDPQFDLFR"
     gene            complement(3395..4108)
                     /locus_tag="AC159U_04965"
     CDS             complement(3395..4108)
                     /locus_tag="AC159U_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336441.1"
                     /translation="MKNLYNSMLNKSLAVFLFLNLSCAIVTINVYFPAEEVKDVFQAL
                     EDELLEDGEVQTDTEADTDQEVTPDATESESEPTSHLYPSEPAVTANKIIATKRVFQL
                     VPEVYAQGNLTQKILNNIKSNPQVQKAYDRRNGRLSDVNKILSKRLAGEGNQGKLVKR
                     GTLSSSDTQTVSDENSDRDIIINAMAEEIVKINKLEMTDDNVSSVKAQAAEQFAAVRR
                     GEVKSGTLIQSSNGQWSAK"
     gene            complement(4124..7528)
                     /locus_tag="AC159U_04970"
     CDS             complement(4124..7528)
                     /locus_tag="AC159U_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3336442.1"
                     /translation="MFLALQYFIPYKIKNYLNDNPQWKGNNLKAENIEYSLLSGFHFT
                     NLEISKPDNKNEFVKINNVYVDIGFLSSIYNRKVSIEHIEIDSIDSAVTEQFLKDTSG
                     YGAPSTTGNDKQRLQLEIQELVIKNFDIAYEQETNFKLTGLILKLGDLQNLKDAKLRS
                     KIEFLGREFSLQSNIIRTKDDINILVDLQTPEFVLSDNENTAIPALNINSIIDLKING
                     EKKSKTNVQIHEEQELIGKADIDIVYKESDKTVIINDSVLEIFNLIKLGFRGDVTNIS
                     DDPTLDITANVENLDIEKFTSLFTDKYNIKLSGNLIKNEIKLAGSIDESLTIKNLFNF
                     DNFLIKQGSDELEIGKLDIVLENKLSSKDNNVNIKASSNSFAGGSINLNSSIELGEDN
                     YAINGRLSGNKFDLGKIFYVNNLITGSVINLDTRFTGNTKTLNFLSDISANNVSIKLS
                     DTQNVNVSHLNSSNKIQTSVSFDNAKERGIVDAEINNLSYKNLDYVSFKIDTGKFNAD
                     VKDLLNDRNYNLNITGDRLENNFLDVTLDSVNISVNEDEGDMITGNITSPSGSYDKYK
                     LSDLKSDYSFTNTDLNFSDITFILIDRGRAKINEVSLPIEEEIEFPSKVNLKKGTFTS
                     DDNGYKLVGINMNLNNNSKETWTGNVTIKEADIYSVIFTKLASIVSYDSRGYKLSSIK
                     GNLLGGKLTGFLNNLNNNLRFNINLSKPKIEGKENIITTNKLNLTYKGKFKNEESLPT
                     GSGKVNVSNMYVEDYEEESKLNINLDIDTESETLSVNNGLISNSNNDVLLEFTSNIEN
                     PYSGERKLEYKTEKMNLAEIKKFANPYLPPAIRFGEISGSMDVELTASNFPNDSTVTG
                     YIDFKKINFKGDPFGTLFNIENLDGRLKLSQDETEVKSINEVIKRHTIDHKKAYKFTK
                     NISRSDESNVNIKRLEYGFFTLENIKLLMNVVKNNLTIKELSTNVFGGKVSSSGVYNI
                     GKSKKKAGKFYFLFNDISLKNITDSIPGTEGYISGRLNGLVSLVQKEKRTSTLDGLFN
                     FWTVGSRKEKRIVGKAFLERLGARERLLLGSSKNYDKGRLYGYINNGVITFNELEISN
                     SILGLKDLNIKVHNRRNSISVAHLMSVIRETARRASTGSLDLQFQN"
     gene            complement(7640..8017)
                     /locus_tag="AC159U_04975"
     CDS             complement(7640..8017)
                     /locus_tag="AC159U_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336443.1"
                     /translation="MKKLILITSILIFTCSFSVSYGISDDAANSFEEFVSNPSEYDKK
                     EIVVEGIINKLKFTSAEGEQYTLFKIEDNSENILHVYYQGKHLDVKKGSVVKVVGEFL
                     KKKRYSFYSFKNVVKAKDVIIET"
     gene            complement(8022..8753)
                     /locus_tag="AC159U_04980"
     CDS             complement(8022..8753)
                     /locus_tag="AC159U_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3336444.1"
                     /translation="MTKKSQSINYNSRLNVEILSTLYNKTDPKVILKNNQSGAVNTYS
                     IGDSFNYLNETFKVLHISECLVSLIGGEQTVNITCREIERDYRVVRVSSLSQFRVVSY
                     DDIFTKSGDFKTDIDQVIKRIGHKYGVDPLLVKAVIKAESNFDPKAVSPKNAQGMMQL
                     IPATAKDYGVSDPFNATQNIDGGVKFLRDLIKFFKGDVDLVLAAYNAGPGTVMKYNYK
                     IPPYPETKNYVQKVKKFYIMYRNRG"
     gene            8954..9742
                     /gene="thiD"
                     /locus_tag="AC159U_04985"
     CDS             8954..9742
                     /gene="thiD"
                     /locus_tag="AC159U_04985"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3336445.1"
                     /translation="MPKALTIAGSDSSGGAGIQADLKTFTAHKVYGMSVLTSITAQNT
                     KKVLGIHNLSPEFIGLQLVAVATDFEVNAVKTGMLSTAPIIKSISDQIKRYKIPNLVV
                     DPVMISKSGARLLEKEAETSLKNDIIPIAFIVTPNIPEAEVIAGMEIGTIEQMKEAAE
                     KIIAMGPKNVLIKGGHLKDNKDAVDIFYDGEDFYEIATKWIKTKNTHGTGCTYSAAIC
                     ANLAKGLDVLEAVSKAKDYVTSAIEKSFNIGSGHGPLNHFHNIK"
     gene            9757..>10069
                     /locus_tag="AC159U_04990"
     CDS             9757..>10069
                     /locus_tag="AC159U_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HIR3336446.1"
                     /translation="MAELIDGKVVSQSVRDEVQELTSKLMDDTGIVPGLAAVLVGDDP
                     ASEIYVRNKRKACEKVGIYSEEHKLPAETTEEDLLALVNKLNDDPKIHGILVQLPLPD
                     HI"
BASE COUNT         3104 a         1807 c         1749 g         3409 t
ORIGIN      
        1 acaatattat tttttatcag ttagaaggtg atcacacaag gcaaaaaatt aattttgcgg
       61 gtttgaagtg gcaatttagt ctgaagtgtg agtgataaaa agattcaaaa atttaaattt
      121 ctattgatat gaaaatcccg cgacaatata gcctagttaa attcaatatg gagaatttaa
      181 aaaagaagta tcatagtaaa taccctttta ctagtgatgg ggtttatgta tattttggag
      241 agattcaaaa tatgccgggt cattgtgtgg tatgtgattt taaatcaggg cagatttata
      301 cgggttatca tattgaaaac tttatagaaa ttagtgaaga aggatatgac agttagttta
      361 tgttataatt ttaagtaaaa gtttagagaa aaatgtgatg gcgacacaaa aagaaatttt
      421 aaaagaagca attaaactta agcctactga aaaggcaaag ttaatagatc agcttatttt
      481 atcattagat aaacctgata gtgatttaga taaattatgg gctgaagaag cagaatcaag
      541 actagaagct tataaaagag gcgaattggt tgcagtttct cttaaagaag tagtagctaa
      601 atatctatga aggtaaagtt tctggttgaa gctcaactcg agctagatga aactataaac
      661 tattataatc aggaagtagc aggtttaggt gatagttttt taagagaaat attgagtacg
      721 attgatagaa taatagaatt tcccaattca tggcatttgc tttctaatga aataagaaga
      781 tgtcaaacaa gaaggtttcc ttatggtttg atttattcaa ttattgataa tgaattatta
      841 gttttatcag tatcaaatct acaccgtaga ccaaatcact ggagagatag aataaaggaa
      901 taaaatcatg aaattctgca cagtaataaa ttgtatggat gggcgggtac aactgcctgt
      961 aattaaatat ctgcaagagc ggtttggagc agagtatgta gattcgatta cagagcctgg
     1021 gcctaatagg atcttggctg agcagactaa tactgctcta ctagattcca tatctacaag
     1081 actaaaaatc tctttaggga atcacgagtc tattggaata gctgttgccg gccatcacgg
     1141 atgtgccgga aatcctaccc caaaagatga gcaaacccag catactctca atgctgttaa
     1201 gtttctcaaa tcaaaatatc caggcgtaga aattatcggc ctctgggttg atggaaattg
     1261 ggaagtggaa gagatacagt tataatttat attagaatac aggcaaggag agagtgttga
     1321 ctaagttact cgaacaggcg tttaataagg cttctaaact tccaaaggaa gaacaagata
     1381 ttcttgcaaa agccatactt gatgaattgg aagcagagca aaaatgggaa gcgctttttg
     1441 aaaaatcagg tgatctcctt gaagaactgg caaaagaagc cttggaagaa cacaaggcag
     1501 gaaaaactaa gtcccttaat cctgaccaac tgtgaaatct ttcacaactg acaagttcag
     1561 gtcgttattt gctaaacttc caaagtctat aaaacaacgg gccagagaat cctatcgtct
     1621 tttcaaaaac aatcctcacc atcctagtct gcattttaag accgtccatc taactgaacc
     1681 gatatactct gttaggatta gtaaggacta tagggcagtt ggtattatca atgacgaaaa
     1741 aatattctgg ttttggatag gaccacattc tgaatatgat caactattga aaaactatca
     1801 aaaaaattaa ttttaataat gtccacaatc caaatcaaca aaaacgatat ccattatctc
     1861 tcaaacacca aaattgacgg tagtcatcca accttgttct tgctccatgg cgctgggcag
     1921 agccacttaa cctgggaata tcaattagat tatctgcaag ctttagatgg gtataacttt
     1981 gtcattcctg atttaccagg tcatggtaaa tcagatggtg agggtcttaa atcagtcgag
     2041 gaatatacag aatttataaa agagttgata agtgaactgg acttaaaaga gataatcttc
     2101 atcggtcatt ccatgggcgg agcagttgcc cagctattag cattagaaga acctgattac
     2161 ttaaaggcta tcattcttgt atgcaccggg gcttcaatga gcgtagcaag agaaacgctt
     2221 ttggctgtaa gagataacta tgaagttttt tgtgaagtag caccaactag agcgtttgct
     2281 gaaagttcac cagaaatatt gaagacaaag ttcaaggaag gtttgcaaaa caccaaacaa
     2341 aaggtggcct acgatgacct actggcttgc aatgtgtttg atatttcaga taagatttca
     2401 ggcataaata tacctacatt aattattgct gggagcgagg atatattaac tattccaaaa
     2461 agaagtgagt atcttcacca tcagattttt gactcggagc tgcatatagt agaaggttca
     2521 gggcattttg taatgcagga gaagactgag gaatttaacg cgctcgtatt tgattttatt
     2581 gagagccttt aaaaataatt atctgaaaag atcgaactga ggatctctta taagtgactt
     2641 tgatccgaca tcttcaaatt cataatcata tccccttaca atcacaaccg gaactgcatc
     2701 gttctttccc ataagcagac ctgccgcgga agctatttca tcacctattg caattagagt
     2761 cgcagtaagc tcataacctt ccttatcctt ttgattcctg taatccttta gagggttaat
     2821 cccccatgta ccaatcgcta tttctgtaag cccttcccgc catggcctgc caactgtgtc
     2881 agtaataatt actgctacgt tctttccgga ttcatttgtt acatcttttg cgattttctt
     2941 tgcagagcca tcagagtcat ctggcagcaa agttacctta tcatttcctg gtacgtttga
     3001 ggcatctatc ccggcattcg caagtacaag cccatcttta ttttcaacca ccatcacacc
     3061 cgtgtcagtc tttctctgca tcttaacgat gcgctttgtt tcaccaagaa taacctcaac
     3121 cattctgggg tcttttagaa gttcgtcacc aagtttctct gcaaatggag tgggtgttat
     3181 atcatttaaa tcgacaactt tcccttcgga tttggatatg attttttggg caataacaag
     3241 aatatccttt tctgctaatt ctatatcaga ggatttaagt ctattagcaa taatccgggc
     3301 taagttatct ccctctttaa tttcagggat accagtaact gggataagtt caagcttaga
     3361 ggacataatc tatgtagaag ttaataatat agatttattt agcagaccac tgaccattgg
     3421 aagactgaat caatgtgccg gatttaacct caccccttct aactgcggca aattgctcgg
     3481 ctgcctgtgc ttttacgctg ctaacattat catctgtcat ttccagttta ttaattttca
     3541 caatctcttc tgccatggca ttaataataa tatccctgtc agaattttcg tcacttacag
     3601 tctgggtgtc tgaagatgaa agagttcctc tttttactaa tttcccctgg ttaccttcac
     3661 cggcaagtcg tttacttaga attttattaa catcactcaa gcggccgttc cttctgtcat
     3721 atgccttttg tacttgagga ttgctcttga tattatttag aatcttttgt gtgagattgc
     3781 cctgagcata cacttctggt actagctgga ataccctctt ggtagcaatt attttgtttg
     3841 cagtaacagc aggctccgaa gggtatagat gtgatgtagg ctcactttca gattccgttg
     3901 catctggagt aacttcctga tctgtgtccg cctctgtatc ggtttgcact tcgccgtctt
     3961 ccaacagctc atcttccaaa gcctgaaata cgtcttttac ctcttctgcg ggaaagtaaa
     4021 catttatagt tacaattgcg catgataggt ttagaaataa aaatactgct aacgatttat
     4081 ttaacatact gttgtataag tttttcataa ctgactcctc attttaattt tgaaattgta
     4141 aatccagact acctgtactt gctctccttg cggtttctct tatcactgac ataagatgtg
     4201 ctacggagat tgagtttctt ctattatgaa ctttaatatt caaatctttc aagcctaata
     4261 tcgaatttga aatttcgagt tcattgaatg taatcactcc gttattaatg tatccgtata
     4321 acctgccttt gtcataattt tttgacgaac caagtagaag tctttctctt gctccaagtc
     4381 gctccaaaaa tgccttcccg acaatcctct tctcttttct tgatcccaca gtccaaaaat
     4441 taaacagtcc atcgagtgta ctggttcgct tttctttttg cactaggctt acaagaccgt
     4501 ttaatctgcc gcttatgtaa ccctcagtac ccgggatgct gtctgttata ttctttaaac
     4561 ttatatcatt aaacagaaaa taaaacttgc ctgctttctt tttacttttt ccgatattat
     4621 agactcctga agaagagact ttaccgccga atacattggt gcttaattcc ttaattgtaa
     4681 gattgttctt tacaacattc atgagcagtt tgatattttc cagagtaaaa aaaccgtatt
     4741 caagcctttt tatattaaca ttggattcat cactgcgaga aatatttttc gtaaacttgt
     4801 atgctttctt atgatctatt gtgtgtcttt ttattacttc atttatgctt ttaacctctg
     4861 tttcatcctg gcttagtttt aatctgccat caaggttttc aatattgaaa agagtgccaa
     4921 acgggtcgcc tttaaagttt attttcttaa agtcaatata tcctgttacg gttgaatcgt
     4981 ttggaaaatt gcttgctgta agctcaacat ccatactacc gcttatctca ccaaatctga
     5041 ttgccggcgg gagatacgga tttgcaaatt tcttgatttc tgcaagattc atcttttctg
     5101 ttttatattc caattttctt tcaccgctat aaggattttc aatattgctt gtaaactcaa
     5161 gcaaaacatc attattgctg ttacttatta aaccattatt tacagacaaa gtttcgcttt
     5221 cggtgtctat atccagattg atattgagct tcgattcctc ttcataatct tctacataca
     5281 tattgctaac atttactttt ccgctaccag tcggtaacga ttcttcgttt ttaaactttc
     5341 ccttataagt taaattcagc ttgtttgtgg ttataatatt ttcttttcct tcaatttttg
     5401 gtttgctaag attaatattg aatctcaaat tattattcag gttatttaaa aagccagtta
     5461 gttttcctcc gagcaaattt cctttaatag aacttaattt atatccacgg gaatcatatg
     5521 aaactataga ggcaagtttg gtaaatatca ccgaatagat gtctgcttct ttgattgtta
     5581 catttcctgt ccaggtttct tttgaattat tgttaaggtt catattaatc ccgacaagtt
     5641 tgtaaccgtt gtcatcagat gtaaaagttc cctttttaag atttaccttg gatgggaatt
     5701 ctatttcctc ttcaatggga aggctaacct catttatctt tgctctacct ctgtctataa
     5761 gtataaaagt aatatcagaa aagttgagat cagtgttagt aaaactgtaa tcacttttta
     5821 aatctgataa tttgtatttg tcgtaactgc ctgagggaga ggtgatattg cctgttatca
     5881 tatctccttc atcttcattt actgaaatat taactgaatc aagagttaca tctaaaaaat
     5941 tattctctaa cctatctccg gttatattca ggttatagtt cctatcattc agaaggtctt
     6001 ttacatcggc attaaatttc ccggtatcta ttttaaagct gacatagtcc agattcttgt
     6061 atgaaagatt gttaatttca gcatcaacta ttcctctctc ttttgcatta tcaaacgaga
     6121 cgctggtttg aattttgttt gatgagttta aatgggaaac atttacattt tgtgtatcag
     6181 ataatttaat agaaacatta ttagcagaga tatcacttaa aaaattcagc gtcttggtgt
     6241 ttcctgtaaa acgcgtatcg agatttatca cacttcctgt aataaggttg tttacataaa
     6301 atatttttcc aaggtcaaat ttatttccac tgagtctgcc gttaatcgca tagttatctt
     6361 cacctaattc aatactggaa tttaagttaa tgctgccacc tgcaaaacta tttgagctgg
     6421 cttttatatt tacattatta tctttggaac taagcttatt ttctaaaacg atatctagtt
     6481 tgccaatttc cagttcatcg ctcccttgtt ttattaaaaa gttatcaaaa ttgaataggt
     6541 ttttgatggt caaactttca tctattgatc cagctaactt aatttcgttt tttatcagat
     6601 tcccactgag cttaatattg tatttatctg tgaacagact tgtgaacttt tctatatcta
     6661 aattttcaac atttgctgta atatctaaag ttggatcatc tgagatattt gtcacatccc
     6721 ctctgaatcc taatttaata agattaaata tttctaatac tgaatcatta ataatcacag
     6781 tcttatcgct ttctttgtaa actatatcaa tatctgcttt tcctataagc tcttgttctt
     6841 catgtatctg tacattggtt ttagatttct tttctccatt aattttcaga tcaattattg
     6901 aatttatatt gagagcggga atagcggtat tttcattatc agacaatacg aattcaggag
     6961 tttgcagatc aactaagata tttatatcat ctttggttct tatgatatta gactgtaaag
     7021 aaaactctct tcctaaaaat tctattttag agcgcagctt tgcgtccttt aaattttgca
     7081 ggtcacccag tttaagaatc aggccagtca gtttaaaatt agtttcttgc tcataagcta
     7141 tatcgaaatt tttaataacc agttcttgta tttctagttg caatctttgc ttatcattgc
     7201 ctgtagtact tggtgctccg tatcctgaag tatcttttaa aaactgctct gttacagcgc
     7261 tatcaatgct atcaatttct atatgctcaa tagatacctt gcgattatat atggaactca
     7321 aaaatccaat atccacataa acattgttga tctttacaaa ttcgttctta ttatcaggtt
     7381 ttgaaatttc taaatttgta aagtgaaaac cggatagcag actgtattcg atattttctg
     7441 ccttcaggtt attacccttc cactgagggt tatcattcag gtaattttta attttatagg
     7501 gaataaaata ttgtagagct agaaacaaaa agcaaagaat aacgagagta aatattaaga
     7561 aatactttag gcgtgcctgt aatttatttt gcgttaagat cattaattat tagtataaga
     7621 tcaaaccgct aaatagaaat taagtttcaa ttattacgtc tttggctttt accacatttt
     7681 tgaagctgta gaatgaatat cttttcttct ttaaaaactc tccaactact tttactactg
     7741 atcctttctt tacatccagg tgcttgccct gatagtatac atgtaatata ttttctgaat
     7801 tatcctctat tttaaacagt gtatactgct cgccttcggc agacgtgaat tttagcttgt
     7861 ttataatgcc ttcgacgact atttcctttt tatcatattc ggaagggttt gatacaaatt
     7921 cttcaaaaga attggctgca tcatcactta ttccgtaact aacgctgaac gagcaggtaa
     7981 agattaaaat agaagttatt aaaattaatt ttttcatatc atcatcctct gtttctgtac
     8041 attatgtaaa acttttttac cttctgcaca taatttttgg tctccggata tggaggaatt
     8101 ttatagttat atttcatcac ggttcccggg cctgcgttat aggctgctag cactagatct
     8161 acatcgcctt tgaaaaattt gataaggtca cgtaaaaatt tcaccccgcc atcaatattt
     8221 tgtgttgcat taaagggatc ggaaaccccg tagtcttttg ccgttgcggg aatgagctgc
     8281 atcatgccct gggcattttt tggagagact gccttgggat caaaattgga ttcggctttt
     8341 ataactgctt taacaagcag tgggtctact ccgtatttat gccctattct tttaattacc
     8401 tgatctatat ctgtcttaaa atcaccggac tttgtaaaaa tgtcatcgta actaactacc
     8461 ctgaattgag acaatgaaga tactcttaca accctgtaat ccctttctat ttccctgcat
     8521 gtgatattaa cagtctgctc accgccaatt aacgatacaa gacactctga aatatgaagc
     8581 accttaaagg tttcattcag ataattaaaa ctgtctccaa ttgagtatgt atttacagct
     8641 ccggattgat tattttttaa tattaccttt gggtcggttt tattgtatag agtactcaga
     8701 atctctacat tcaatctgga attataatta atcgattgcg atttttttgt cacatatgcc
     8761 agatcttgtg aaattgcggc ataatttgag gagaaaatta ttaatagtgc taaaattgtg
     8821 gttttcatgc taatttataa ataaataatt aatatattaa ttataggcta tttttgaaca
     8881 aatttaaagt agtttattaa gttttatttg taaatagata aattatttgt aaaattccca
     8941 gatgaaatcc gatattccca aggcacttac cattgcaggc tctgatagca gcggaggtgc
     9001 cggaatacag gctgatctta aaactttcac tgcacataaa gtatatggta tgtcggtgct
     9061 cacttcaatc acagctcaaa ataccaaaaa agtacttgga attcataacc taagccccga
     9121 gtttatagga cttcagcttg tggcagtagc aacggatttt gaagtaaatg cagtaaaaac
     9181 agggatgctt tccactgcac cgattattaa atctatttct gatcagataa agagatacaa
     9241 aattccaaat cttgttgtag accctgtgat gatatcaaaa agcggtgcaa gacttttgga
     9301 aaaagaggct gaaacatcgc ttaaaaatga tataataccg attgctttta ttgtaacgcc
     9361 taatattccg gaagctgaag ttatagccgg tatggagatt ggcactatag aacagatgaa
     9421 agaagccgct gagaagatta tagctatggg cccaaaaaat gtactcataa agggtgggca
     9481 ccttaaagac aataaagatg ctgtagacat tttttatgat ggggaagact tctacgagat
     9541 tgctacaaaa tggattaaaa caaaaaatac gcacggcaca ggatgcacat attctgctgc
     9601 aatctgtgct aatcttgcaa aaggacttga tgtacttgaa gccgttagca aggcaaagga
     9661 ttatgttaca tcagcaatag aaaaatcatt taatatagga agcggccacg gcccgcttaa
     9721 tcattttcat aatataaaat aaaaggaagg aaagttatgg cagagcttat agatgggaaa
     9781 gtagtatcac agagcgttag ggatgaagtg caggagctta caagcaaatt gatggatgat
     9841 acgggaatag tccccgggct tgcagcggtg ctagtaggtg atgatcccgc atctgagatt
     9901 tatgtaagga acaagagaaa ggcatgtgaa aaagtaggta tatactctga agaacacaaa
     9961 ctgcctgctg aaaccaccga ggaagacttg ctcgctttag taaataaact aaacgatgat
    10021 ccaaagattc acggtatatt ggttcagctt ccactacccg accatatta
//
LOCUS       DBNGSV010000141         6421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1065354, whole genome shotgun sequence.
ACCESSION   DBNGSV010000141 DBNGSV010000000
VERSION     DBNGSV010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1065354"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC159U_04995"
     CDS             <1..186
                     /locus_tag="AC159U_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336447.1"
                     /translation="LIKAEKYLPESVYVQFELGKTFKEIGNKEKMEYRFNKALNLPNT
                     DHRDKYFKKHITKYLEE"
     gene            complement(452..2422)
                     /locus_tag="AC159U_05000"
     CDS             complement(452..2422)
                     /locus_tag="AC159U_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR3336448.1"
                     /translation="MPKDIPVSNGSFLLNFDSDYQIRDIYFPFIGQENHSGGHPFRFG
                     VWSDGQYSRMGPEWKKDLRYHDNSLVTDVVLKNDDLGLELRCSDVVDLDLNIYIKKIN
                     VSNLTDKARQVRLFFSHDFNLYGNDIGGTAYFDPRSCSIIHYKIHRYFLISCWAHEQW
                     GVKHFSCGRKEHLGNLETITETNSGELNGEASAWGSPHSTIGIWLKLPAMGKATAYYW
                     MAAGVTYPEVTKLNLEVHEKTPEQLIVRTSRYWKSWVHNDSLQLDSLPKSVTDVYNRS
                     LLILRTQIDNNGAIIAANDSDIIRFGKDTYSYMWGRDGAFVAAALAKAGYSHVCMKYF
                     NFCARILSDEGYLFQHYNPDGSLASNWHSWLVDGKEVLPIQEDSTALTLWALWIHYKS
                     LNDVEFIRPLYKTLIKKIAEFLVSYRDPQTLLPQPSYDLWEERYALHAYTVSCVIAGL
                     RAAANFAILFQDTFLAEKYDSVADELTEGISQHLYHDGLKRFARSGTRTDRGYKLDEV
                     IDISLLSLVTLGVFDAKDPRMIETAKAVREELWLKTPVTGCARYQGDVYQRADDSPKD
                     IPGNPWFISTLWLAEYFIDQAENHEQLHATLPYLEWCGKNALPSGVLAEQIHPVSGLP
                     LCVSPLTWSHSAFVWTVLRYVEKNDVLNKNSF"
     gene            complement(2431..3597)
                     /locus_tag="AC159U_05005"
     CDS             complement(2431..3597)
                     /locus_tag="AC159U_05005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3336449.1"
                     /translation="MTKKSGKINVLHVATLNRPIRQDLGYGPIETVIYNIDKGLHKLG
                     HRSIVACSGDSCVVGEKFTTIEKSFSEYWSKDTCIQRENMRKHLLLSLDRAKKGDIDI
                     IHMHDAIMVEYIFNGVLDCPVPIVMTLHVPAEDKGPFKRWNESLISTSGVYFVPISEY
                     QSKQHEGLVNAQNVIHHGIDVEDYPYMSSSDKHKYLFSIGRITQDKGQDKAIEIAKKT
                     GSRLILAGNIQNKAKDRAFFKSLKKSIDLVVDAGKPPFNDNYYEKVMKPILDSDKQII
                     YIGEVDSVRKKQWYSHAQATLFSIQWGEPFGLVLIESMACGTPVLAFNKGSVPEIVVD
                     GETGFIVDSVDGMAEAVSKIHLINPGDCHKHVKNNFSIASMAEKYSELYHQIIH"
     gene            complement(3912..3988)
                     /locus_tag="AC159U_05010"
     tRNA            complement(3912..3988)
                     /locus_tag="AC159U_05010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3952..3954),aa:Arg,seq:ccg)
     gene            complement(3979..4743)
                     /locus_tag="AC159U_05015"
     CDS             complement(3979..4743)
                     /locus_tag="AC159U_05015"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3336450.1"
                     /translation="MDSKREELIKYFKQQKELGVEFYFNKTEKADTVHKQIEQLKETN
                     KSAGDTEMPKNTNQMNLLDSTAEKMTLDEISEEIGDCTRCKLHEGRTNIVFGEGNANA
                     RLVFVGEGPGRDEDEQGRPFVGRAGKLLTKIIHAMGLQRTDVFICNVVKCRPPDNRNP
                     EADEMTTCGQFLTKQLISINPEVIVCLGSIASKYLLNHKGSLGSLRGKFHDYQNSKLL
                     VTYHPAALLRNPNFKKPLWEDMKIVLKELQLPVPGD"
     gene            4863..>6421
                     /gene="leuS"
                     /locus_tag="AC159U_05020"
     CDS             4863..>6421
                     /gene="leuS"
                     /locus_tag="AC159U_05020"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3336451.1"
                     /translation="MEENYNPQEIEARWQKVWEEAKTYVATEDSQKEKYYVLEMFPYP
                     SGRIHMGHVRNYTIADVIARYKRTMDLNVLHPIGWDAFGLPAENAAIKNNLHPAKWTK
                     ENISQMREQLKRMGFSYDWTRELATCDSSYYRWEQLVFIKMFEKGIAYKKSSQVNWCP
                     SCETVLANEQVEEGLCWRCSSEVQLKEMDGWFFKITDYAEELLESINSLKDGWPERVL
                     LMQQNWIGKSSGAEIDFPLESPLDDVKSLPIYTTRPDTLFGVTYMCIAPEHPLVDKLI
                     NGKENEDKAREFISRIKKQSNIERISEGANKEGVFTGSYCINPLSNEKVPIYIANFVL
                     MDYGTGIIMSVPAHDQRDFEFAKQYDLPIKVVISPQDKEVKSDDLEEAFVDSGVMVNS
                     SLFDGTPSNEGKEKVINYLEENIIGTRQINYRLRDWGISRQRYWGAPIPMINCEKCGT
                     VAVPEKDLPVELPLDIEFSGKGGSPLENIEEFVNVKCPKCGSDAKRETDTMDTFMESS
                     WYFMRYASPNY"
BASE COUNT         1932 a         1261 c         1281 g         1947 t
ORIGIN      
        1 cttataaaag ccgagaagta tcttcctgaa agtgtgtatg ttcagtttga acttggaaag
       61 acttttaaag agataggtaa taaggaaaag atggaatatc gttttaataa agcactaaac
      121 cttccaaaca ccgaccacag ggataagtat ttcaagaagc atataacaaa gtaccttgaa
      181 gagtaactgt tagaaaatcg tatgaaaaaa tggcgggcga tgggggattc gaaccctcga
      241 ctcctggctc cggagatcat tttgagcaat tttctaagta tctgctaaca ttcaatagtt
      301 ctctaagttc ctaaaatcat aaaaattaaa atctgagcat atatttataa tgagtgcaat
      361 acatacattc tgtttacaaa taacgttttc tttaaattag gtcttcgttt attaatttct
      421 agttacagtt tgtcttatca ttcatacttt tttagaaact gtttttattg aggacatcgt
      481 ttttttcaac atatcgcagg actgtccaaa caaaagcgga atgactccag gtaagaggtg
      541 aaacacaaag aggcaaaccg cttacaggat gtatctgttc ggcaagtaca ccagaaggaa
      601 gcgcattttt tccgcaccat tcaagatagg gaagggttgc atgaagctgt tcatgattct
      661 ctgcctgatc aataaaatat tcagctaacc aaagagtaga gataaaccat ggatttccag
      721 ggatgtcttt tgggctatca tcagcgcgct gatagacatc accctggtat ctggcacaac
      781 ctgtaacagg agttttaagc caaagctcct cacgtacagc cttggctgtt tcaatcattc
      841 gaggatcttt agcatcaaaa actccgagag tcaccaggct taacaggctg atatcaataa
      901 cttcatctag tttataaccc ctatctgttc tggtacctga acgtgcgaat cgctttaagc
      961 cgtcatggta aaggtgttga ctgatcccct ccgtcaattc atctgccacc gagtcatact
     1021 tctcagccag gaaggtgtct tgaaagagta ttgcaaaatt cgcagcggct ctgaggccgg
     1081 caatcacaca tgatacagta taggcatgca gtgcataacg ctcttcccaa agatcataag
     1141 agggttgtgg cagcaatgtt tgcgggtccc tgtaactaac taaaaattca gctatttttt
     1201 ttatgagtgt tttgtacaga ggcctgataa attctacatc attcaaactc ttatagtgta
     1261 tccaaagcgc ccaaagtgtt aaagcagttg aatcttcctg gataggcaga acttctttgc
     1321 catcgaccaa ccaggaatgc cagttgcttg caagggatcc atcgggatta taatgctgga
     1381 acaagtagcc ttcgtctgac aaaatacgtg cgcaaaaatt aaaatatttc atgcatacat
     1441 gagagtaccc tgccttcgct aaagcggctg caacaaaagc gccgtcacga ccccacatat
     1501 acgaataagt atcctttcca aatcgaatga tgtcagaatc attcgctgct ataatggcac
     1561 cgttattatc aatctgggtt cttaatatga gcaggctacg gttataaaca tcggtcactg
     1621 atttgggtag gctatctagc tggagcgaat cattatgaac ccatgatttc cagtatctcg
     1681 atgtgcgaac gatcagttgc tcaggtgttt tttcatggac ttccaagttg agcttcgtaa
     1741 cttccggata tgttactccc gccgccatcc aataatatgc ggttgctttc cccatggctg
     1801 gcaatttcag ccaaattcca atagtggaat gaggcgagcc ccatgctgat gcctctccgt
     1861 taagctctcc actatttgtt tctgtaatgg tttctaaatt tcctaaatgt tcttttctcc
     1921 cacagctgaa atgtttcacg ccccattgtt catgcgccca gcagcttatc agaaagtacc
     1981 ggtggatttt gtaatggatt atcgagcagc tccgtgggtc aaagtaagcc gtgccaccta
     2041 tatcatttcc atataaatta aagtcatgac taaaaaaaag tcgaacttga cgcgctttgt
     2101 cggtgaggtt cgatacatta atttttttga tatagatgtt caaatctaaa tccaccacat
     2161 ctgagcatcg gagttctaaa cccaaatcat cattttttaa aacgacatct gttacaaggc
     2221 tattatcatg atatcttaaa tcttttttcc attcaggtcc catccgggaa tattgtccat
     2281 ccgaccaaac tccaaaacga aaggggtgtc cgcccgaatg gttctcctgt ccgataaaag
     2341 gaaaataaat atcacgaatc tgataatccg aatcaaaatt caatagaaat gatccgttac
     2401 ttacgggaat gtcttttggc ataagctcct ttaatgaata atttgatgat aaagctcaga
     2461 atatttttcg gccatagagg cgattgaaaa attatttttc acgtgtttat ggcagtcacc
     2521 gggatttata aggtgaatct tactaacagc ctcagccatg ccatcaacag aatccactat
     2581 gaagccggtt tctccatcaa caacaatttc tggcaccgaa cctttgttaa aagcaagcac
     2641 cggtgtgcca catgccatcg attcaataag taccagcccg aacggttcgc cccattgtat
     2701 agaaaaaagc gtagcctgcg cgtggctata ccactgtttt ttccgtacac tatcaacttc
     2761 tccgatataa ataatctgtt tatcggaatc cagaataggc ttcattactt tttcataata
     2821 attgtcatta aagggaggtt tgcccgcgtc aacaacaaga tcaattgact ttttcaaact
     2881 tttaaaaaaa gccctgtctt tcgctttgtt ttgtatatta ccagcaagga tgagacgtga
     2941 gccagttttc tttgcgatct ctatggcttt atcctgtccc ttatcttgcg tgattcttcc
     3001 aatcgagaat aaatatttat gtttatctga actactcata tagggatagt cttctacatc
     3061 aataccatga tgaatgacat tctgggcatt tactaaaccc tcatgctgtt tactctgata
     3121 ttcacttatc ggtacaaaat agacacctga tgtagaaatg agcgactcat tccagcgttt
     3181 gaacggtcct ttgtcttcgg caggtacatg aagagtcata acaatgggga cagggcaatc
     3241 aagtactcca ttaaaaatat attcaaccat aatggcatca tgcatgtgta tgatatctat
     3301 atcacccttc tttgccctat caagcgatag taagagatgt ttacgcatgt tttcacgctg
     3361 tatgcatgta tctttactcc agtactcact gaaactcttc tcaattgtag tgaatttttc
     3421 tccgacaaca caggaatcac ctgaacaggc tacaatggaa cgatgtccta atttatgaag
     3481 tcctttgtct atattataaa ttactgtttc tataggaccg tagcccaaat cttgtcttat
     3541 tggccggttt agtgtagcta catggagcac atttattttt ccactctttt ttgtcatggt
     3601 gtaatttcaa taaaaatata aggacagtaa tcaggcaagt ttttttaaca tagcctccat
     3661 agaagcatta agtaatatta aattatttaa tacattccac gttttataag acaaataaat
     3721 agagtgtcat taggcaggct gccaaataat agagggtaaa tatactgtac ttggtaggat
     3781 ccttctatct aagttatcat tataattata ccttataaga agggtaaata ctatttatat
     3841 taaatagagg atagaaaacc atttataaac cttataaatg gtataaatgg ggatgctacc
     3901 ctttattggt ttggcgggcg atgggggatt cgaacccccg acccctggct ccggaggcca
     3961 gtgctctaat ccactgagct aatcacccgg tactggtaat tgtaactctt ttagtactat
     4021 tttcatatct tcccagaggg gttttttgaa attcgggttt ctcaaaagtg ctgcaggatg
     4081 atatgtgaca agaagtttgg aattctgata gtcatgaaac tttcccctga gacttccgag
     4141 gcttccctta tggttcagta aatactttga tgcaatactt ccaaggcaga caattacttc
     4201 ggggtttata gatataagct gtttagtaag aaactgcccg catgtggtca tttcgtctgc
     4261 ttcggggttt ctgttatccg gtggtctgca ttttacgacg ttgcaaataa agacatctgt
     4321 gcgctgcagg cccatggcgt gtataatttt agtgagcagt tttcccgctc ttccaacaaa
     4381 tggtcttccc tgctcgtcct catcccttcc ggggccttca ccaacaaata ctaacctggc
     4441 attggcattg ccctcaccaa aaacaatatt agttcttccc tcatgtaatt tgcaccttgt
     4501 acaatcgcct atttcttcac taatttcatc caatgtcatt ttttctgctg tgctatcgag
     4561 taagttcatt tgattagtat tctttggcat ctctgtatct cctgcagatt tgttggtttc
     4621 ttttaattgt tcaatttgct tatgtacagt gtctgctttt tcggttttgt taaaataaaa
     4681 ctcaacccca agctcttttt gctgtttaaa atattttatt aattcttctc ttttgctgtc
     4741 catagtgtgt aattgataat atcacgtttt gataattaat ttaaatttga ttactgttga
     4801 aagtagtatc agcattattt aatataagcc attcaaaata attatatgtc agggatctgt
     4861 taatggaaga aaattataac ccacaggaaa ttgaagccag gtggcaaaag gtctgggaag
     4921 aggctaaaac ctatgtagct acagaggata gccaaaagga gaaatactat gttttagaga
     4981 tgtttcccta tccatccgga aggattcata tgggccatgt gagaaactat acaattgccg
     5041 atgtgatagc ccggtacaaa aggacaatgg atttaaatgt tcttcaccct attggatggg
     5101 atgcatttgg tcttcctgct gaaaatgctg caattaaaaa taatctacat ccagccaaat
     5161 ggactaaaga aaatatctcc cagatgaggg agcagctaaa gaggatgggt tttagttatg
     5221 actggacaag agaacttgca acatgcgaca gctcttacta caggtgggaa cagcttgttt
     5281 ttattaagat gttcgaaaag ggaattgcat acaaaaaatc atcgcaggtc aactggtgtc
     5341 cgtcatgtga gactgttctt gctaatgagc aggtagaaga ggggctttgc tggaggtgtt
     5401 ccagtgaagt gcagcttaag gagatggacg gctggttttt taagatcaca gattacgcag
     5461 aggaacttct tgaaagcatt aattcactaa aagatggctg gcccgaacga gtactactta
     5521 tgcagcaaaa ctggataggt aagagcagcg gggcagagat agattttcca ctagaatcgc
     5581 ctctggatga tgtaaagagt ctgccaattt ataccacaag gccggacact ctttttggag
     5641 ttacatatat gtgtatagct cctgagcacc cgcttgtaga taaacttata aacggaaaag
     5701 aaaatgaaga taaagccaga gaatttatta gtagaataaa aaagcagagc aatattgaaa
     5761 ggatttctga gggcgctaat aaggagggag tgtttacagg ctcatactgt attaacccct
     5821 tgagtaatga aaaagtgcca atttatatag ctaactttgt actcatggat tacggaacgg
     5881 ggatcataat gtcagttccc gcgcatgacc agagggactt tgaatttgca aagcagtacg
     5941 accttccaat taaagttgtt ataagtcctc aagataaaga ggttaagtca gatgatttag
     6001 aagaggcttt tgttgattcg ggggtgatgg ttaattcgtc actgtttgat ggtactccat
     6061 caaatgaagg aaaagaaaaa gtaataaatt atttagaaga aaacattatt ggtacaagac
     6121 agataaacta ccgtttaaga gactggggaa tatcgagaca gagatactgg ggtgctccta
     6181 taccgatgat aaattgtgag aaatgcggca ctgtagcagt acctgaaaaa gacctgcctg
     6241 tagagcttcc actggacatt gaattttctg gtaaaggggg ttctcctctt gagaatatag
     6301 aagagtttgt aaatgtgaaa tgtcccaaat gcggctcgga tgccaaaaga gaaacagata
     6361 caatggatac ttttatggaa tcctcctggt actttatgag atatgcatcg ccgaattatg
     6421 a
//
LOCUS       DBNGSV010000142         6210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1341746, whole genome shotgun sequence.
ACCESSION   DBNGSV010000142 DBNGSV010000000
VERSION     DBNGSV010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1341746"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(451..741)
                     /locus_tag="AC159U_05025"
     CDS             complement(451..741)
                     /locus_tag="AC159U_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3336452.1"
                     /translation="MKIVWSPLAVERVSEVALYIADDNPSAAERWVDSIFKETERLKK
                     FPILGRVCPEVGKKEIREIIFGNYRIIYRVEEKEIIILTVRHGKQILRTDEL"
     gene            complement(738..1025)
                     /locus_tag="AC159U_05030"
     CDS             complement(738..1025)
                     /locus_tag="AC159U_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012600094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3336453.1"
                     /translation="MKNIDLSEDIRSLSEFRANTSSLIEQVKNTRRPLVITQHGKSSA
                     VVMDVREYESLMDKLELLQDIQTAETQIEAGRGISDSDAKKQVLNKLAVNK"
     gene            complement(1223..2194)
                     /locus_tag="AC159U_05035"
     CDS             complement(1223..2194)
                     /locus_tag="AC159U_05035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3336454.1"
                     /translation="MKAIVFTEYGPPDVVLKLKDVEKPVPKDDEVLIRVHAASVNDWD
                     WNLVRGKPLYIQLFCGLFKPKINIPGVDIAGEVEAIGENVKRLKPGDKVYGDISECGF
                     GGFAEYVCVHEKALALKPESMSFEQAAAIPHAAMLAVQGLHDKGDIRSGQKILFNGAG
                     GGVGTLGVQIAKSIGADITGVDSSEKLEMMRSVGFDHTIDYEKEDFTKTGKHYDLILD
                     TKTNRSVFDYIRVLSPKGTYVTVGGTTARLLEVFILGWIINKLGNKKIRIVALKPNKD
                     LDYINELFKAGKIKPVIDGPYELSEVPAAIEYFGSGNHKGKVIITVG"
     gene            complement(2384..2770)
                     /locus_tag="AC159U_05040"
     CDS             complement(2384..2770)
                     /locus_tag="AC159U_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3336455.1"
                     /translation="MTDSLNSNISLLKKIDIRNLDACADIFSDNFVFHYFNPRLTELE
                     GDYRGVEELKGFFAKLAEMSNDSFNVSVIDANTVGDELVVTQTCNRISLEGNTIEFDV
                     VVVWRIVEGKINEAWDIPSVYHTRLI"
     gene            complement(2948..3367)
                     /locus_tag="AC159U_05045"
     CDS             complement(2948..3367)
                     /locus_tag="AC159U_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336456.1"
                     /translation="MDLKLDIFVPVLKRIICRVESLRSIKRFQKTGIEGWFKVEIVAA
                     LDTRIQSLNNKGPDILFDDNTELEIKAATDFNKSYFINDPIQKYGCPCLFLGDGTKRS
                     ILEEKLDENIEIVAYEIISDDENDWLLGLVKKKGASP"
     gene            complement(3425..4174)
                     /locus_tag="AC159U_05050"
     CDS             complement(3425..4174)
                     /locus_tag="AC159U_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HIR3336457.1"
                     /translation="MIISQNQHGINTTGRGIRAVKIFTRQTLVGMSFRNILPYQTESK
                     DKEDCLWDICSVASLSRNILEGYLSIIYYGTEIISEDDAEFRFLIGQFHRNREWYGIR
                     KHFDPNDSALKDFKLGQKDQKEKIKNHQFISKLTDEQKNKVLKGYDIYKTKADFQKEN
                     KICNGLRRDYQLLSNLVHPLPLSIERIDNESGRGIGSDTDINYCILSLIIARRYLAAS
                     TIEIADFFIDDIGSKFKVKIDTIRPFVLINA"
     gene            complement(4686..>6210)
                     /locus_tag="AC159U_05055"
     rRNA            complement(4686..>6210)
                     /locus_tag="AC159U_05055"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1781 a         1369 c         1045 g         2015 t
ORIGIN      
        1 cttaaatatc ttattatttg ctctggcgtg taacgcttcc tgggcatttc ccttgcctcc
       61 tattcatagg gtttattacc taaaattcta acttgtaatc tggtactatt atagggggga
      121 aggtcaggga acactaaggt aataagcaaa atgatattga gaattaataa gtatctcatt
      181 ttaaattctc ataattaatc aatttttaga atatataaat tgttcagtgc agctacttat
      241 attaatagtc tactaaatga ttgatgtaat tcaaatataa aggttaaatg caaaagcaag
      301 tgcctgtaat tactaaagag acaggttaga acagtgctat aaaaatatta agtaaaagaa
      361 aatgttgttt gtcagtattt actgatgtat accaaactca ttgagcatag ttgaagtgca
      421 ccctgaaaaa agtgggggtt gatcaaggct ttaaagttca tcggtacgca gtatttgttt
      481 gccgtgtctc accgtaagga ttattatttc tttctcttcg actctgtaaa ttattctata
      541 attgccaaat ataatctctc ttatctcttt cttaccgact tcggggcaga ctcggccgag
      601 aataggaaac ttttttaacc tttcagtttc tttaaatata gaatctaccc atctctccgc
      661 agcgctaggg ttatcatctg caatgtataa agctacctcg gagacccttt caacagcaag
      721 gggtgaccag actattttca tttgttaaca gccaatttgt tgagaacctg tttcttggca
      781 tctgaatcac taatacccct acccgcttcg atctgtgtct cggcagtctg gatatcttga
      841 agtaattcta atttatccat gagtgattca tattccctaa catccataac cacagcagag
      901 cttttgccat gctgagtaat cacaagaggt cttctcgtat tctttacctg ctctattaat
      961 gaagaagtat tcgctctaaa ctctgaaagt gatcttatat cctcagataa atctatgttt
     1021 ttcatattgt cctcttaata gtatctttgc ttgtattata tattgtactg aattacggac
     1081 aattaacaaa cccaacttac ttacctcatt gcgaaccgtg cttctctaat ttactaatca
     1141 aaaccttgca cggtgtgaca atctcgcttt taaacccctg aaaaaattgt ctgtttgatc
     1201 aaagaaaaag gaagagtctc cactatccaa cagttatgat tacttttcct ttatggttcc
     1261 cacttccaaa atactcaatt gctgccggaa cttcgcttaa ctcataggga ccatctatta
     1321 cgggtttaat cttacctgct ttgaaaagct cgtttatata atccaaatcc ttgtttggtt
     1381 tcaatgctac aatacgtatt tttttattac ctagtttgtt tattatccac cccagtataa
     1441 aaacctccag aagcctggcc gttgttcccc cgactgtgac ataagttcct ttagggctta
     1501 atacacgtat atagtcaaat accgatcgat tagtcttagt atctagaatc aaatcataat
     1561 gcttgcctgt tttagtgaaa tcttcttttt cgtaatcaat ggtgtgatca aagcctaccg
     1621 agcgcatcat ttccaatttc tccgaactgt ctacaccggt tatatcagct ccgatcgatt
     1681 ttgcaatctg cacacccaga gtgcccacac cccctccagc accgttgaac aatatctttt
     1741 gtcctgaccg aatatcacct ttgtcatgaa ggccttgcac agcaagcatt gccgcatgag
     1801 gtatcgcggc tgcttgttca aagctcatgc tttcaggttt cagagctagt gctttttcat
     1861 gcacacagac atattctgca aaaccgccaa agccacactc ggatatgtca ccatatactt
     1921 tatcacccgg ctttaaccgt tttacgtttt ccccgatcgc ttcaacctca cccgctatat
     1981 caaccccggg tatattgatt ttcggtttaa aaagcccgca aaacagctga atatataatg
     2041 gcttgcctct tacaagattc cagtcccagt cgtttacaga cgccgcgtga actcttatca
     2101 agacttcatc gtctttgggc acaggctttt caacatcttt aagctttaga acaacatccg
     2161 gtggtccgta ctctgtaaag actatggctt tcatatttat tctctttata ctcctctatc
     2221 tggtcagtag aagtatagcc tacccaataa aatcctagcc atgaaaaaag tggttggtta
     2281 ataggatgtg aaaaattagt gtccaatttt aaggcctgac acatttgatt ttctgtggtt
     2341 gtagttagtc gcctgttgct aaagcttgtt agttttggtc ctgttaaatt aatcgagtgt
     2401 gataaacaga tgggatgtcc catgcttcat taattttacc ttcaacaatc ctccagacaa
     2461 caacgacatc aaattctatg gtattacctt ctaaagatat gcggttacag gtctgtgtta
     2521 cgacaagttc atctccaaca gtgtttgcat caatcactga aacattgaaa ctatcattac
     2581 tcatctcagc tagctttgca aagaaaccct taagctcctc aacaccacgg tagtctcctt
     2641 ccaattcagt cagccttgga ttgaaatagt gaaagacaaa attatctgaa aagatatcag
     2701 cacatgcatc cagattgcgg atatcgattt tctttaaaag ggagatattt gagttaagtg
     2761 aatcagtcat aattatgctc ttgttaattg ttaatgtaat aaattattgt tcttttcaca
     2821 attaaattat ataaaaaatt aatatttact attgattaaa aagtatataa cgttaggaca
     2881 tccacccgct tttatacgcc ctgaaaaaag agattgggtg gtgaggagtg aaaaatagtg
     2941 tccaatgtca aggactggca ccttttttct tgacaagacc taacaaccaa tcattttcat
     3001 catcactaat aatttcatag gcaacaattt ctatattttc atctagtttt tcttcgagta
     3061 tgctacgttt tgtaccgtca cccaagaata agcatgggca tccatatttt tggatcggat
     3121 catttataaa ataactttta ttaaagtcag tggcggcctt tatttcaagc tcggtattat
     3181 catcgaatag gatatctgga cctttattat tcaacgactg tattcttgtg tctaatgcgg
     3241 caacgatttc tactttgaac caaccttcaa tacctgtctt ctgaaatctt ttaatactac
     3301 gaagtgattc aactcgacaa ataatccttt ttaatactgg cacaaaaata tctagtttta
     3361 aatccattaa aatatttacc tcataactat ggtgccataa gaataactag ttagactttc
     3421 ataattatgc attaattaaa acaaatggtc taatagtatc tattttcact ttaaatttag
     3481 atccaatatc atcaataaaa aaatcggcaa tttcaattgt gcttgctgcc agatatctcc
     3541 ttgcaataat taaacttaag atacaataat ttatatcagt atcacttcca atacctcttc
     3601 cactttcatt atctattctt tctatagaaa gaggtagggg atgaaccaag tttgatagta
     3661 attggtaatc ccgtcttaga ccattacata ttttattttc tttctgaaaa tccgctttag
     3721 ttttgtaaat atcgtaccct tttaaaactt tgtttttttg ttcatcagtc aatttggaaa
     3781 taaattgatg gttttttatt ttttcttttt gatctttttg tcctaatttg aaatctttca
     3841 atgcagagtc attaggatca aaatgttttc tgataccata ccactctcga tttcggtgaa
     3901 attgtccaat taggaaccta aattctgcat catcttctga aattatttct gtaccgtaat
     3961 atataatgct aagataacct tctagaatat tgcgagataa agaagcgact gaacaaatat
     4021 cccatagaca atcttctttg tctttgcttt cagtttgata gggtaatata ttcctaaagc
     4081 tcattcctac taatgtctgt cgagtaaata tttttacagc tctaatgccc ctgccagtag
     4141 tattgatacc atgctgattt tgggaaatta tcatggttgt atctagaact ttgtcatact
     4201 ttttaagttc ttctaaatat gatatttcat aattgtttaa aactctttta taggtaattt
     4261 tattatttcc ctttcgacag aattttcgtt tttataaaga tgtaatttat aaaattttaa
     4321 atagattttg atgctaatgc ataattatat tgtgcaatta atagtataat atcgaataaa
     4381 aacataataa acagtgacag atgtagatgc tcagtaatta gggataattt ttaatattgt
     4441 aaagaagaaa gcgggatttt aaaattatca ggaaataata atttgataaa tgtagtggga
     4501 aatgaggatt cacttttttt tgcacaaaag tagatttctc tcttgcactc gaaataagta
     4561 agaataaaga tattttggaa taatccggaa ggaaaaatcc tttaacaatc acatatttat
     4621 tacatattac ttttctataa cctgacagaa taaagaaaga aacagcttga gttcttttaa
     4681 accccgcagt taagcctcac gatcagttag taccagtcag ctcaacacct cgcggtgctt
     4741 acacctctgg cctatcaacc ttgtagtctt caagggatct taaggaactt aacgttcggg
     4801 agatctaatc ttagggcagg cttcccgctt agatgctttc agcggttatc ctttccgagc
     4861 attactaccc agcactgccg ctggcgcgac aactggtaca caagaggctc gtccactcag
     4921 gtcctctcgt actatgagca gcccccttca aatctcctac gcccactgca gatagggacc
     4981 gaactgtctc acgacgttct aaacccagct cgcgtaccgc tttaatgggc gaacagccca
     5041 acccttggga ccttctccag ccccaggatg cgatgagccg acatcgaggt atcaatcccc
     5101 gccgtcgatg tggactcttg ggcgggatta atctgttatc cccggagtac cttttatccg
     5161 ttgatcgatg gcctttccac acagaaccac cgggtcacta tgacctactt tcgtatctgc
     5221 tcgacctgtc ggtctcacag tcaagctccc ttatgccatt gcactctacg cctggtttcc
     5281 aatcaggctg agggaacctt tgtgcgcctc cgttactctt taggaggcga ccgccccagt
     5341 caaactaccc gcctaacagt gtcctcaacc cggattcacg ggttcaagtt agaattccag
     5401 cactacaagg gtggtatttc actgaagtct ccacactacc cagaagcaat gtttcacaga
     5461 ctcccaccta tgctacgcat ggaataccaa aattcaatgc taggttatag taaaggttca
     5521 cggggtcttt ccgtcccgca gcgggtaccc ggcatcttca ccggaactac aatttcactg
     5581 agtctctggt cgagacagcg tggcaatcgt tacacctttc atgcaggtcg gaacttaccc
     5641 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ccggggcttc
     5701 agttcagagc ttctccgaag ataacccttc cctttaaccg tccggcaccg ggcaggtgtc
     5761 agaccctata cttcggctta cgcctttgca gagccctgtg tttttagtaa acagtcgcca
     5821 ccacctattc actgcgacct gcttcagctc cagcagtaaa tgctttcacc taatgcaggc
     5881 accccttctt ccaaagttac ggggcaaatt tgccgagttc cttgaccaga gttcactcac
     5941 gcgccttagt attctcaact cacccacgtg tgtccgttta gagtacgagc accttataat
     6001 caacactacg aggtttttct cgtaagcatg ggatcaatca ctttatgagg ccgaagcctc
     6061 tcgtactcac ctctcaggct taacgaggga cggatttgcc tatccctcac cctacaggct
     6121 tgaaccggga tatccatcac ccggctgacc tacccttctc agtcacccct tcgctcatat
     6181 aaggtggtac aggaatatta acctgttttc
//
LOCUS       DBNGSV010000143         3921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1272810, whole genome shotgun sequence.
ACCESSION   DBNGSV010000143 DBNGSV010000000
VERSION     DBNGSV010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1272810"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="AC159U_05060"
     CDS             <1..404
                     /locus_tag="AC159U_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336458.1"
                     /translation="NNTRDYTQLIGSLTTGASLRCPDRVNNPCEESDALVDSSFFEAL
                     PEAGDGETATLADCANPKAGYCISMGDLAEIGSTDFDFGWEASFRSFDKTGRRDFSVF
                     GDGTIRCTLDSSLAKGATGAFGSKRSDSGCD"
     gene            569..1168
                     /locus_tag="AC159U_05065"
     CDS             569..1168
                     /locus_tag="AC159U_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3336459.1"
                     /translation="MNKVKTQLQQKGFTLIELLVVAAIIGILLAIAIPNLIKARISAN
                     EANARKSTQALRDAEYQYFEGDLDDNGIRDFTDQIGSIAQSASLRCPMIPSGESECAE
                     FDALVDKSFERMLSIGGSEGDCIDPKAGYCLKFDETITGSLLEADFGWQLTPASVNKS
                     GRKDFAGFGDTVIRCTVSTLATGSRGAFEANQSSPACID"
     gene            1241..2164
                     /locus_tag="AC159U_05070"
     CDS             1241..2164
                     /locus_tag="AC159U_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3336460.1"
                     /translation="MVLRVDSLIKDFRKGFFRKKTRVLKNISFEVNQGEIFGVIGPNG
                     AGKTVTFKSVMGFISPTSGNIKIFGQDNSKYLLKEKLGYLPENPYFYEYLTGFELLNY
                     MGSLHGIHGKHLLDRINYLLDRVNLTKSAGVQLRKYSKGMLQRIGIAQAIINDPDFVI
                     FDEPMSGLDPLGRREVRDLILELKNNGKTVILSSHILSDLEALCDRVCFIFHGEVVKQ
                     GNLADLLSEIESGYEAIIRKNSKVTQQLFGNNVVIEQRGEFIALIFDEQHKNNILQSI
                     HSNQIDLVSFHPIRKSLENIFNIHSVIDKQN"
     gene            2178..2993
                     /locus_tag="AC159U_05075"
     CDS             2178..2993
                     /locus_tag="AC159U_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336461.1"
                     /translation="MLHTINRIKVVAYNTFLEAVRDRILYSLLIFAVLMILGTIIISS
                     ISAEQYNKITKDFGLTTISIIGILISCFLGMNVVYKEIDRKTVYNLFSKPVRRFEFVL
                     GKYLGISFTLFVITLGMSIILFVLVYYVENRYKGLIEFHYGGQYYAQFVAALYFTFLE
                     FLIIIAISLVFSSFTTPIMTVMFVLLVFIIGRFSSDIKLFSQHVNHPLVGYITEGVYR
                     LVPHLDQFNLRNQAVYGGDINLSLFLNLSSYALLYCFAMIVLSIAIFEKKEFK"
     gene            2971..>3921
                     /locus_tag="AC159U_05080"
     CDS             2971..>3921
                     /locus_tag="AC159U_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336462.1"
                     /translation="MRKKNLNKMLSKNLTTIGVLLSLLLLIASVPVQKKIEEIRGDYS
                     IISGSLLLSSKTLKKLSLGYNLLIADIYWIRALQYFSDKKFDVNKPQELFKYFDIMTD
                     LDPKFVNAYRFGGTFLAEPPELGLGEVELGVKLLDKGRINNPENFRLVLDEAFIYYLY
                     TDNYKRASELFEEASEKPALSELRRASMKGMAALALSKTGNLELSKKIWKYIYTTSTI
                     EARKNHALKNLKKVETKEIENVLTGALGKYYQDNNKLPENIDALVVSGYIKTVPSDPS
                     GGEFIIVSDTRTIVSTTLSENEYKYAISLLNARSKKFNRIY"
BASE COUNT         1272 a          599 c          762 g         1288 t
ORIGIN      
        1 gtaataacac aagagactat acacagttaa taggtagttt gacaacagga gcaagcctac
       61 gttgtcctga tcgggtcaat aatccatgtg aagagtctga cgcgcttgtt gatagtagtt
      121 tcttcgaagc tttacctgaa gctggagacg gtgaaacggc tacattagct gactgtgcaa
      181 atcctaaagc aggttattgt atcagtatgg gagatcttgc tgaaattgga agtacagatt
      241 ttgattttgg ttgggaagct tcatttagat ctttcgataa gacaggaaga agggattttt
      301 ctgtattcgg agatggaaca ataagatgta cactggattc ttcactcgct aagggtgcaa
      361 caggtgcatt tggttctaaa agaagcgact ctggttgtga ttaattcaat agttatataa
      421 agcagaatat acaggcaggc acaacttatt tattgtgcct gtctagtttt agactttatt
      481 acaagtttta aactgtgagt tacttcttta cttactaata cctttttact tgatttatac
      541 taattaaatc tgatataatg atactgttat gaataaagta aaaacacaat tacaacaaaa
      601 agggtttaca ctaatagaat tactggttgt ggctgcaatt ataggtatat tactggcgat
      661 agctataccc aatttaatta aagcccgaat ttctgcaaac gaagcaaatg ctagaaaatc
      721 tacccaggcg ctccgtgatg ctgaatatca gtactttgaa ggcgatctcg atgataatgg
      781 tattagagat tttacagatc aaatagggtc aattgctcaa tctgctagtt tacgatgtcc
      841 tatgataccc tcaggtgagt ccgagtgtgc ggaatttgat gccttagtgg ataaaagttt
      901 tgaaagaatg ctttctatcg gtggttctga gggtgattgt attgacccta aagcaggtta
      961 ttgtttaaaa tttgatgaaa ctattacagg tagtctttta gaagctgatt ttggatggca
     1021 gcttacacca gcaagtgtta ataaaagtgg tagaaaagat tttgctggat ttggtgatac
     1081 agttattcgc tgcactgtaa gtactctggc aactggttca agaggtgctt ttgaagctaa
     1141 ccaatcttct ccagcttgta ttgattaact atttagaatt tttaagtaag aattcacttt
     1201 acttagatat atatcttcct tataatatcc cttctataca atggttttac gagtagacag
     1261 tcttataaag gattttagaa aaggattttt tagaaagaaa accagggtac taaagaatat
     1321 ttcctttgaa gttaaccagg gggagatatt tggtgttata ggtccaaatg gtgcaggtaa
     1381 aactgttact ttcaaatctg taatgggctt tatcagcccc acttcaggta atataaaaat
     1441 atttggacaa gataatagca aatatcttct aaaagaaaaa ctgggatatt taccagagaa
     1501 tccttacttc tatgaatatt taacaggttt tgagctgcta aattatatgg gaagtcttca
     1561 tgggattcat ggaaaacatt tattagaccg aataaactat ttactcgata gggttaacct
     1621 aacaaaatca gcaggcgtac agcttaggaa atattctaag gggatgttgc agaggatcgg
     1681 tatagcccag gctattatca atgatcctga tttcgttata tttgatgaac ccatgagtgg
     1741 tctagatcct ctaggacgaa gggaggttag agatcttatc ttggaactaa aaaataatgg
     1801 taaaacagta attctaagct cccacatcct ttctgatctg gaagctttat gcgacagggt
     1861 atgttttatt tttcatggtg aggttgtaaa gcagggaaac cttgctgatt tgttatctga
     1921 aatagaatcc ggctatgaag caatcataag aaaaaatagt aaagttaccc agcaattatt
     1981 tggtaataac gttgttattg aacaaagagg agaatttatt gcactaatat ttgatgaaca
     2041 acataagaat aatattttac aaagtataca cagcaatcag attgatttag tatcatttca
     2101 tccgattaga aaatcacttg aaaatatatt taacatacac tcagtgatag acaaacagaa
     2161 ttagcaagca ggttattatg ctccatacaa ttaatagaat aaaagttgtt gcctataata
     2221 cctttttaga agccgttaga gacaggatat tgtattcgct tttgatcttt gctgttctga
     2281 tgattcttgg gactattata atttcaagca tctccgctga acaatataat aaaattacaa
     2341 aagacttcgg tcttaccacc atatcaataa tcgggatatt gatatcatgt tttttgggaa
     2401 tgaatgttgt ttataaagag atagacagaa agacagttta taaccttttc tcaaagcctg
     2461 ttagaaggtt cgaatttgtt ttaggtaaat atctgggcat ttctttcact ctctttgtaa
     2521 taacactcgg gatgtcaatt atacttttcg tattagttta ttatgtggag aatagatata
     2581 aggggttaat agagtttcat tacggagggc agtactatgc tcaatttgtt gcagcacttt
     2641 atttcacttt tcttgagttt ttaataataa tagcaataag ccttgtattt tccagtttta
     2701 ctacacccat tatgaccgtg atgtttgttc tgcttgtatt cattattgga agattttcat
     2761 cagatattaa gttgttttcc cagcatgtta atcacccttt agttggctac attacagaag
     2821 gagtctacag gctagtacct caccttgatc agtttaattt aagaaaccag gcagtgtatg
     2881 gtggggatat aaatctttca ctttttctaa atctatcatc ctatgcactt ttatactgtt
     2941 tcgctatgat tgttttatca atagcaattt ttgagaaaaa agaatttaaa taagatgctt
     3001 agtaaaaatc ttactaccat aggcgtattg ctatctcttt tgttattaat tgcctcggtt
     3061 cctgttcaaa aaaagataga ggaaatcagg ggggattata gcattatcag cggttctctt
     3121 ctcctgtctt caaaaaccct taagaaatta tcactgggat ataatttgct catagccgat
     3181 atctactgga taagagcctt acagtatttt tctgataaga agtttgatgt gaataaaccc
     3241 caagagttat tcaaatattt tgatatcatg acagatctcg atcccaaatt tgtaaacgca
     3301 tacagatttg ggggaacttt tcttgctgaa cctcctgaat tggggcttgg cgaggttgag
     3361 cttggggtaa agttactgga taagggaagg attaataatc ctgaaaattt tagactagtg
     3421 ctggatgaag cttttatcta ctatttatat actgataact acaagagagc ttctgagcta
     3481 tttgaagaag cttctgaaaa acctgcatta tctgaattga ggagagcttc aatgaagggt
     3541 atggcggctc ttgctctgtc taaaactgga aatctcgaac tatcaaagaa aatctggaaa
     3601 tatatttata ccacatctac aatagaagcc agaaagaatc atgcattaaa aaatttaaag
     3661 aaagttgaaa ccaaagagat tgaaaatgta ctaacaggtg cacttggaaa gtattatcag
     3721 gacaataaca agctgcctga aaatatagat gcgcttgttg tatcgggata tattaagact
     3781 gttccctctg atccatcggg aggagagttc attatcgtat ctgatacaag aaccatcgtc
     3841 agcactacac tatctgaaaa tgaatataaa tatgctatat ctttattaaa tgcaaggtcg
     3901 aagaaattta ataggatcta t
//
LOCUS       DBNGSV010000144         7606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_718676, whole genome shotgun sequence.
ACCESSION   DBNGSV010000144 DBNGSV010000000
VERSION     DBNGSV010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_718676"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /gene="acpS"
                     /locus_tag="AC159U_05085"
     CDS             complement(<1..288)
                     /gene="acpS"
                     /locus_tag="AC159U_05085"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR3336463.1"
                     /translation="MIDGLGVDLVKIERIKHIIDKWDKKFTAKLFTENEIQYCSKHKN
                     QYLRFASTFAAKEALIKAHGRGNLKFKEIEILREISGRPQVNLHGNALKIIN"
     gene            complement(290..1012)
                     /locus_tag="AC159U_05090"
     CDS             complement(290..1012)
                     /locus_tag="AC159U_05090"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3336464.1"
                     /translation="MPNLNVNIDHVATLRQARLGHEPDPVFAAYLSELAGADGIVVHL
                     REDRRHIQERDVKILKKTVKTKLNLEMAPTKQMLKIALQIKPDMVTLVPEKRQELTTE
                     GGLDVICNKKTIGNIVKSLKDSAIITSLFIDPDTKQIDASKQLMSDMVEIHTGKYADS
                     INEEQQNFELNEIINSVNYASELDLRVAAGHGLDYSNTSKIAEINKIEELNIGYNIIC
                     RSVIVGIERAVKEMKQLCTGKT"
     gene            complement(1005..2366)
                     /gene="glmM"
                     /locus_tag="AC159U_05095"
     CDS             complement(1005..2366)
                     /gene="glmM"
                     /locus_tag="AC159U_05095"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3336465.1"
                     /translation="MKIFGTDGVRGLANTEPMTPETVLKLGKSLVHYLRINLRIKSPK
                     ILIGKDTRLSGYMFEQALSAGFISMGASVYLVGPLPTPAIAYLTRNINADAGVVISAS
                     HNPYTDNGIKFFGSDGFKLEDIAENIIEDILVNKKYQQIDHSQIGKAHRIDDASFRYV
                     SYLKNTFPNNHNLSDLKIVVDCANGAGYKVCPQILKELGAQVTVLSDNPDGKNINLNC
                     GALFPKELCKKVIETGSDIGIALDGDADRCVFVDHHGDVVDGDRVIALCINEMLSQKD
                     IKDDIFVFTQMSNMALQNYVDSKNAKFILTDVGDKYVVDQMKKCNSMFGGEKSGHYVF
                     MEHSTTGDGILSALQILSIMKKNNKTLSDLSNILNLYPQTLVNIKVKDKPPFETISNL
                     NESLCKCNKMLGKNGRINLRYSGTEQLARVMVEGENEQSIQEIASELANIINKEIGVN
                     YDA"
     gene            complement(2374..3303)
                     /locus_tag="AC159U_05100"
     CDS             complement(2374..3303)
                     /locus_tag="AC159U_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR3336466.1"
                     /translation="MKEYITNNIGKKILAITIALILWIIANFEQDIEKDISIDIQYND
                     LSPELIITNNPPEALNMRIRGSRTKLSILKTDNYSYPVPLTNVNKGLSKFNIRPQQIR
                     IPGVQILGISPSEIDVQVDNLITKRVKVKPNIGLPDIGYELIGTPKVEPNTVKIGGPK
                     DLISKLDSINTDEIKIEGEKTNFTIEVPLKANSPLITIEDNKEVVKITVDIQETMIEK
                     EFKELGISIIDTNNKIIEIIGTQTVDILFKGPYSKIKELKSEDIKITSSIKNILSQTN
                     MKHKIKLKVDYPNSEDINLEQITPGTVDIIIKQ"
     gene            complement(3313..4077)
                     /gene="cdaA"
                     /locus_tag="AC159U_05105"
     CDS             complement(3313..4077)
                     /gene="cdaA"
                     /locus_tag="AC159U_05105"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3336467.1"
                     /translation="MFEFISNYRYVQDTLDIVFVAFIIYFVLRFIEGTRAVQMIFGLL
                     SLVIIYFLSQNFDLFTINWLLGNFLSFIILIAVILFQHDLRRVLAAIGRSPFLLHFHP
                     VKSNKSIVDEIVKSASYLSNNKIGALIAIERNNNLGDFVEIGTEIDAILSTQLIISIF
                     NPASPLHDGAIIVSNEKILSAGSFFPLATDPDLDKDLGTRHRAAIGVTRETDCVVVIV
                     SEETSKISLASQGELIRAMDASSLNQKLLEILELNK"
     gene            complement(4083..4907)
                     /gene="folP"
                     /locus_tag="AC159U_05110"
     CDS             complement(4083..4907)
                     /gene="folP"
                     /locus_tag="AC159U_05110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3336468.1"
                     /translation="MISGLLKQNRTLIMGILNVTPDSFYDGGKYISLNNALKHSEQLI
                     EDGADIIDIGGESTRPGSNPVSENDELNRVIPVIAEINKKFKIPISVDTTKAQVARQA
                     LDNGASIVNDISGLNFEPQIADIVSKYNASIVLCHTSSRPVDMQTKINYDDLILNIYN
                     YLQDSIKLCEHKGINSDNIYIDPGFGFGKSVKDNLLLLKSLPVFKKLNKKILVGTSMK
                     SFIGKTLGTDSLEIKSDGTFATVVISILNGADIVRVHDVKKMKTAVKMTEAVINIQ"
     gene            complement(5001..6842)
                     /gene="ftsH"
                     /locus_tag="AC159U_05115"
     CDS             complement(5001..6842)
                     /gene="ftsH"
                     /locus_tag="AC159U_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3336469.1"
                     /translation="MINNQLFKNILLWLVIFVGIIALYQFVQTPRSTIKEIQYSEFVS
                     YIKNQEIHEVEFSEFEVTGNFVNKNITPNKFKAIGPTTEDILKLLEENNITFSFAQKR
                     ENSLSHILLNWAPLLLLIGIMVLFMRQIQSGGTKAMSFGKSRARMLTEDKNKTTFKDV
                     AGIDEAKEEVKEIVDFLKSPKKFTDLGGRIPKGVLLVGPPGTGKTLLAKAIAGEAGVP
                     FFIISGSDFVEMFVGVGASRVRDLFIQAKKNAPCIIFVDELDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFESNEGIIVMAATNRPDVLDPALLRPGRFDRQVIVPRPDVRGRQ
                     EILNVHVKNNPLDDDIDLAVIARSTPGLSGADLENLVNESALHAARIGNHKITMDDFE
                     FAKDKVLMGVERRSMIISDDEKKITAYHEGGHALVAKLTPGSDPIHKVTIIPRGLALG
                     VTQQLPIEDKYTHSKSYLNKRIMVLLGGRAAEEIIFNERTSGAANDLEVATDIARKMV
                     CEWGMSEELGPVKLGKSDEQVFLGKEMSKHNNYSETTALQIDNEIKSIVSTNYDKAAK
                     LLKDNINLLHKLADMLLDKEVVDSKELESLVKGFDPSNNMFGGTVST"
     gene            complement(6967..>7606)
                     /locus_tag="AC159U_05120"
     CDS             complement(6967..>7606)
                     /locus_tag="AC159U_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3336470.1"
                     /translation="DVSYETIQVLKTYETEIVLTPAKDSIEGSIKRSWEIASETKDSF
                     IPNQFENPNNPESHETTTALELLEQIPGDIHALVLSVNTGGSISGIGKVLKHKYPDIR
                     IYAVEPQESAVLSGQKPQKHNIKGIGPGFIPKVLNTEIYDDILKIKSDEAKSFTRELA
                     TKEGLLVGISSGAVAKAAKMVAEKLGRSKNVATIFSDSGQRYLSTDLFDMFQ"
BASE COUNT         2187 a         1391 c         1193 g         2835 t
ORIGIN      
        1 gttaattatt tttagcgcat ttccatgtaa attgacctga ggtctaccag aaatttctct
       61 aagtatttca atttctttga attttaggtt tcctcttccg tgggctttta ttaatgcctc
      121 ctttgcagca aaagttgacg caaagcgaag atattggttt ttatgtttac tgcaatattg
      181 aatttcattc tctgtaaata attttgcagt gaatttttta tcccatttat ctattatgtg
      241 tttaatcctt tcaatcttaa ctaaatctac tccaagacca tcgatcatat taagttttac
      301 ctgtacaaag ctgtttcatt tctttaaccg ctctttctat acctactatt actgatctgc
      361 aaatgatatt atatcctata ttaagttctt ctattttatt tatttcggca attttacttg
      421 tgttgctgta atcaagcccg tgtcctgcag ctactcttag atctagttca gaagcataat
      481 taacagagtt tattatttcg tttagttcaa agttttgttg ttcttcattt atactatctg
      541 catatttgcc ggtatgtatt tcaaccatat cagacataag ttgtttagat gcatcaattt
      601 gttttgtgtc aggatcaata aacagacttg ttataattgc tgaatccttg aggcttttaa
      661 caatatttcc aatagttttt ttattgcata ttacatccag gccaccttcg gtagtaagtt
      721 cctgcctttt ttcaggcaca agcgtaacca tatcgggttt tatttgaagt gctatcttta
      781 acatttgttt ggttggggcc atctcaaggt taagtttggt tttaacagtc tttttcaata
      841 tttttacgtc tctttcctgg atgtgtcttc ggtcttctcg aagatgaacg acgataccat
      901 ccgcacctgc aagttcacta aggtaagcag caaaaaccgg atctggttca tggcccagtc
      961 ttgcttgtct taaagtagcc acatgatcaa tatttacatt caggttaggc atcgtaattg
     1021 actccaattt ctttattaat gatgttagct aattcagatg caatctcttg tattgattgt
     1081 tcattctctc cttcaaccat aactcttgca agctgttctg taccagaata tctaaggtta
     1141 attcttccgt ttttacccag catcttattg cacttgcaaa gtgattcatt caaatttgat
     1201 attgtttcaa aggggggttt atcttttact ttaatattaa caagagtttg tggatataag
     1261 tttaagatat ttgatagatc agataacgtt ttattattct ttttcattat agataggatc
     1321 tgtagtgcag atagtatgcc gtcacctgtt gttgaatgct ccataaatac atagtgaccg
     1381 gatttttcac caccaaacat tgaattacat tttttcattt gatcaacaac atatttgtct
     1441 ccaacatctg ttaaaatgaa ttttgcattc ttgctatcta catagttttg tagcgccata
     1501 ttgctcatct gcgtaaaaac aaatatatca tcttttatgt ctttttgaga tagcatttca
     1561 tttatacata gtgcaattac tctgtcccca tcaactacat ctccatggtg gtcaacaaat
     1621 acgcatctat ctgcatcacc atcgagtgca attccgatat ctgagccggt ttcaattact
     1681 tttttacaaa gctcctttgg aaaaagagca ccgcaattga gatttatatt tttcccgtca
     1741 gggttatccg aaagtactgt aacttgggca ccaagctctt taagtatttg cggacagacc
     1801 ttgtatcctg ccccatttgc acaatcaaca actattttta aatcagataa attatgatta
     1861 tttggaaaag tattttttaa atatgataca tatctaaaag aagcatcatc aattctatga
     1921 gcttttccaa tttgtgaatg atcaatttgt tggtatttct tattcactaa aatatcttct
     1981 attatatttt cagcaatatc ttctaattta aagccgtcgg agccaaagaa tttaattcca
     2041 ttatcggtat aaggattatg tgatgctgat ataactacac ctgcatctgc attaatattg
     2101 cgtgtaagat aggctatagc gggagtgggt agtggcccta caaggtaaac agaagcaccc
     2161 atagagataa aacctgcaga cagtgcctgt tcgaacatat aacccgagag tctagtatcc
     2221 ttaccaatca gaattttggg agatttaatg cgaaggttaa ttcgtagata atgaacaaga
     2281 gatttgccca gtttaagaac tgtctccggc gtcataggtt ctgtatttgc aagtcctctt
     2341 actccgtctg ttccaaatat tttcatcttg atattattgt ttaattataa tatccacggt
     2401 ccccggcgtt atttgttcga ggttaatgtc ttcagagttt ggataatcta cttttagttt
     2461 gattttatgt ttcatgtttg tttgagaaag tatatttttt atagacgaag taattttaat
     2521 atcttcgctt tttaattctt taatcttgct ataaggccct ttaaatagaa tatcaactgt
     2581 ttgtgttcct atgatttcta taattttatt attagtatct ataatactta taccaagttc
     2641 tttaaattct ttttctatca ttgtttcttg tatatcaact gtgattttaa ctacttcttt
     2701 attgtcttct atagttatca gcggactatt agcttttaaa ggtacttcaa ttgtaaaatt
     2761 tgttttttct ccttcaattt tgatttcatc agtatttata gagtctaatt tagatataag
     2821 atcttttggg ccaccaattt ttactgtatt aggctctact tttggagtac caataagctc
     2881 atatccaatg tcaggtagac caatatttgg tttaacctta actcttttag ttataagatt
     2941 atcgacctga acatctattt cagaagggct tattcccagt atttgcactc caggaattct
     3001 tatttgctga ggtcttatat taaatttaga taaaccctta tttacatttg taagtgggac
     3061 ggggtaagag tagttatcgg ttttaaggat agacagttta gttctcgaac ctcttattct
     3121 catatttaag gcttcaggcg ggttgtttgt aattataagt tcaggagaga ggtcattgta
     3181 ctgaatatct attgatatgt ctttttctat atcttgctca aaattagcaa taatccataa
     3241 aattaatgct atggttattg ctaatatttt tttgcctata ttattagtta tatattcttt
     3301 catttatatt aattatttat ttagctctag aatttctaat agtttttgat ttaaggatga
     3361 tgcatccatt gctcttatta attcaccctg actagctagt gatattttgg aagtttcttc
     3421 agaaactata actacaacac agtctgtttc tcttgttaca ccaattgcag cgcgatgtct
     3481 ggtaccaaga tctttatcaa ggtcgggatc tgtggccaaa gggaaaaatg atccggcaga
     3541 tagaattttt tcattactta ctatgattgc tccatcatgc aggggagaag ctggattgaa
     3601 gatacttatt attaactgag tagatagtat tgcgtcaatt tccgttccaa tctcaacaaa
     3661 atctccaaga ttattattcc tttcaatagc tatcagtgcg ccaattttat tgtttgataa
     3721 atagctagct gattttacta tttcatcaac tatactctta ttggacttaa ccggatgaaa
     3781 atgcagaaga aaagggcttc ttcctatagc ggccagaacc ctgcgaaggt catgttgaaa
     3841 tagaattacg gcaatgagga taataaagct aaggaaatta cccaagagcc agtttattgt
     3901 aaaaagatcg aaattttgag atagaaagta aataataact agtgacaata gaccgaatat
     3961 catctgtaca gcccttgtac cctctataaa gcgcagaaca aagtaaatga taaaggccac
     4021 aaagactata tctaatgtgt cttgtacata tctgtaattt gatataaatt caaacattta
     4081 ttctattgaa tatttattac cgcttcggtc attttaacag ctgttttcat tttctttaca
     4141 tcatgaactc taactatatc tgcaccattt agtatagaaa taacaactgt agcaaaagtt
     4201 ccgtccgatt ttatttctaa actatccgta cctaatgttt taccaataaa tgatttcatg
     4261 gacgttccaa ctaaaatttt tttatttaat tttttaaaaa caggcaatga ttttaacaat
     4321 aataaattat cttttactga ttttccaaaa ccaaatcccg gatcaatgta tatattatca
     4381 gaatttattc ctttatgttc gcataattta atagaatctt gtaaataatt ataaatattc
     4441 aatattaaat catcataatt tatctttgtt tgcatatcca cgggtcttga tgatgtatgg
     4501 cataaaacta ttgatgcatt atattttgaa acaatatctg cgatctgggg ctcaaaatta
     4561 agaccactta tatcattaac tattgatgca ccgttatcta aagcctgtct tgctacttgt
     4621 gcttttgttg tatctacaga tatgggaatt ttaaattttt tattaatttc agctattaca
     4681 ggaattactc tgttaagctc atcgttttca ctaaccgggt tagaaccggg tctggttgac
     4741 tctcctccaa tatcaataat atctgcacca tcttcaataa gttgttcact atgtttaagg
     4801 gcattattta atgatatata ctttcctcca tcataaaaag agtcaggagt tacgttcaat
     4861 atgcccatta tgagggttct attttgtttt aataaaccgc ttatcatata ttgataaaaa
     4921 tatacaactg tactaaatat attacagaag aaaaggcaat aatactttaa ttggatgcag
     4981 attaatgaag taaaactaat ttatgtacta acagtaccac caaacatgtt gttactggga
     5041 tcaaagcctt ttacaaggct ttcaagctct ttactgtcta ctacttcctt gtcaagaagc
     5101 atatcagcta atttatgaag cagattgata ttatctttta aaagtttagc tgctttatca
     5161 tagttagtac ttacaatact ttttatttca ttatcaattt gcagggcggt agtttcgctg
     5221 tagttattat gttttgacat ttcttttcct aaaaatacct gctcatctga ttttccaagc
     5281 ttaaccggcc caagctcttc actcattccc cattcgcaaa ccatttttct tgctatatca
     5341 gtggctacct caaggtcatt ggcagcgcca ctggttctct cattaaatat aatctcttca
     5401 gctgcacggc ctcccagaag aaccataatt cttttattaa gataggattt agaatgagta
     5461 tatttatctt caattggcag ctgctgtgtt acgccaagag ccagacctct tgggatgatg
     5521 gttaccttgt gtatgggatc agatccagga gtgagtttgg ctactagggc gtgaccacct
     5581 tcatgataag cagtaatttt cttttcatca tcactaatta tcatgcttct tctttcaaca
     5641 cccattaaga ctttatcctt tgcaaattca aagtcgtcca ttgtgatttt atgattacca
     5701 attcttgccg catgaagggc tgattcgtta acaaggtttt caaggtctgc acctgatagt
     5761 cctggtgttg atctggcaat taccgcaagg tctatatcat catcaagagg attatttttt
     5821 acgtgtacat tcaagatttc ctgtctgcct ctcacatcgg gtctgggaac tattacctgt
     5881 ctgtcaaatc tgccaggtct tagaagagct ggatcaagta catcaggtct gtttgtggct
     5941 gccataacaa taattccttc gtttgattca aacccgtcca tttctacaag taactggttt
     6001 aaagtctgtt ctctttcgtc atgaccaccg cctagtccgg cacccctgtg tcttcctacg
     6061 gcatcaagtt catctacaaa tattatacag ggagcgtttt tcttggcctg tatgaacaag
     6121 tctcttaccc tagaagcgcc aactccgaca aacatctcta cgaagtcact accacttata
     6181 ataaaaaacg gtacaccagc ctctccggct atggcttttg caagtaatgt tttacctgtt
     6241 cccggaggtc ctactaaaag tacgcccttt ggtatccttc caccaaggtc ggtgaatttt
     6301 ttagggctct ttaaaaaatc tactatttcc ttaacttctt ccttagcttc gtcgatgccg
     6361 gcaacgtctt taaaagttgt tttgttctta tcctcggtaa gcattcttgc tctgcttttg
     6421 ccaaaagaca ttgccttggt accacctgac tgtatttgtc tcatgaaaag taccataata
     6481 ccaatcagta agagtagtgg ggcccaattt aacagtatat gagataggga attctctctt
     6541 ttttgtgcaa agctaaaagt tatgttgttt tcttccagaa gctttaggat atcctcagtg
     6601 gtgggtccaa tagccttgaa tttatttggt gtaatattct tgtttacaaa atttcccgtt
     6661 acttcaaatt cactaaactc aacttcatga atttcctgat tttttatata acttacaaac
     6721 tcactgtatt gaatctcttt tatagtagat ctgggtgttt gtacaaactg ataaagagct
     6781 ataataccaa caaagatcac aagccataat agtatattct taaataattg attgtttatc
     6841 atgtttaatt attaccgtgt ataaattgaa ttttggttaa cttaaaaaag atatcatatg
     6901 caacattcat tatcaaataa agtaactact aaaaaatata attcaagcct ttgtaacttg
     6961 ctgtaattat tgaaacatat caaataggtc tgtgctcaag tatctttgtc cactatcact
     7021 gaatattgtt gccacatttt ttgatctgcc cagtttttcg gcaaccattt ttgctgcttt
     7081 agctacagct cctgaggata tacctacaag aaggccttct tttgttgcta attctcttgt
     7141 gaaactcttt gcttcatcgc ttttaatctt taatatatca tcatatatct cggtatttaa
     7201 tactttaggt ataaaacctg gaccgatacc tttaatattg tgtttttggg gcttttgacc
     7261 ggaaagtact gcactctcct gtggttctac ggcataaatc cggatatcag gatatttgtg
     7321 cttaagaact tttccaatgc cacttattga tccgccggta tttacagata aaaccaaagc
     7381 atgaatatcc cctggaattt gttctaataa ttcaagagcg gttgttgttt catgtgactc
     7441 gggattattt ggattttcaa actgatttgg tataaatgaa tcttttgttt ctgatgcaat
     7501 ttcccatgat ctttttattg aaccttcaat actatcttta gcaggagtga gaactatttc
     7561 agtttcatat gtttttaaaa cctgtattgt ttcataactt acatca
//
LOCUS       DBNGSV010000145         1165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_511504, whole genome shotgun sequence.
ACCESSION   DBNGSV010000145 DBNGSV010000000
VERSION     DBNGSV010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_511504"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..846)
                     /locus_tag="AC159U_05125"
     CDS             complement(190..846)
                     /locus_tag="AC159U_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072697709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR3336471.1"
                     /translation="MQKKLLNNSKLSNNKIAKLVQMFALEVPACKAAVALNINRNTAQ
                     RVYTRIRLAIVKHCERHSPFSGEVEVGESYFGGKRKGNRGRGASAKVPVFGLLKRNGK
                     VYTRIVKDVSGSTLKQIIRNKVDTDSVIYSDSFRSYDGLVAYGYKHKRISHDKRFGVK
                     TNHINGIENFWGFAKTKLRRYNGIDRKHFVLYLKEMEFRFNNRNTDLVNKIKEILSST
                     "
     gene            913..>1165
                     /locus_tag="AC159U_05130"
     CDS             913..>1165
                     /locus_tag="AC159U_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336472.1"
                     /translation="MKEYQLAWQDSGTLSHLKTSFDSGVYVFIYEQNPNRVIYVGTAT
                     FKKGFTNRWAEHLSHFNEGGRTAWRPNLDQDIYSLMSRKN"
BASE COUNT          317 a          233 c          178 g          437 t
ORIGIN      
        1 tatcaagata tgaatactta cctccgttgt aaaaagaatc cggtgttaca ttcaatatac
       61 ccattatgag agttctctgt tctataggga gtctggtatc ctttaaataa agggttgcca
      121 taatataata tgtcttactg aaccttgagc ttagttaatt tatactttag gagctcgcat
      181 agaacatgtc tatgtactac taagaatctc cttgatttta ttaactaaat ctgtatttct
      241 gttgttaaat ctaaattcca tctcttttaa atacaaaaca aagtgttttc tgtcaattcc
      301 attgtatctt ctcaacttcg ttttagcaaa gccccagaag ttctctattc catttatatg
      361 atttgtcttt actccaaatc tcttatcatg actaatcctt ttatgtttat acccataagc
      421 tacaagacca tcatatgatc taaaactatc cgaatagatt acgctgtctg tgtccacttt
      481 attcctgata atttgcttta atgtacttcc cgatacatct tttactattc ttgtgtatac
      541 cttgccgttt ctttttagca gcccaaatac aggtacttta gcacttgctc ctcttcctcg
      601 atttccttta cgctttcctc cgaaatatga ttcacctact tctacctctc cgctaaatgg
      661 agagtgtctt tcacagtgtt taactattgc taatcttatt cttgtataga ctctttgtgc
      721 tgtgttacgg tttatgttca atgcaactgc tgctttacat gctggtactt ccagtgcaaa
      781 catttgtacc agcttagcta tcttattatt tgataacttt gaattgttga gtaacttctt
      841 ttgcatagtc tcagcttaat atatcttgct gtcctatgcg agctccttat atttttatat
      901 aggaattatg tgatgaaaga atatcagtta gcgtggcaag attctggaac actttcgcat
      961 cttaaaacat cctttgattc tggcgtctat gtttttattt atgagcaaaa tcctaatcga
     1021 gtaatttatg tggggacagc aaccttcaag aaaggcttta caaatagatg ggcggaacat
     1081 cttagtcatt tcaacgaagg aggccgaact gcttggcgtc ctaatttgga tcaggatata
     1141 tattccctca tgtcacgcaa aaatg
//
LOCUS       DBNGSV010000146         3505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_650330, whole genome shotgun sequence.
ACCESSION   DBNGSV010000146 DBNGSV010000000
VERSION     DBNGSV010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_650330"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="infB"
                     /locus_tag="AC159U_05135"
     CDS             <1..1038
                     /gene="infB"
                     /locus_tag="AC159U_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3336473.1"
                     /translation="LELILLQADVLELKANPDKRANGIVVESKLDKGRGPLATVIVKE
                     GTLKIGDNIISGTDSGKVRALIDDSGKNMKEAGPSTPAEIMGFSSVPTAGVPFYVVHD
                     DKALKEIVSHRESKVQGKIAGPDRKISLDNLFENLEQGETKELALIIKADTQGSVGAL
                     IDSIEKLSSEKCSVKVVHSAVGAINESDIVLAGASNAIILGFNVRPDPKAIQLAEKQG
                     VSLELHSIIYDAIDRIKNAMEGLLEPIKKENITGHAEVRDTFHVSKIGTIAGCYVTDG
                     KFSRNSNVRLIRDGIVIYDGQLESLRRFKDDVKEVQNGYECGISIRNFNDIKVGDILE
                     NYIIEEEKQKL"
     gene            1046..1336
                     /locus_tag="AC159U_05140"
     CDS             1046..1336
                     /locus_tag="AC159U_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3336474.1"
                     /translation="MVVGVLKIELLIDESRSLKDKRKVVKSITEKVRNKFKNVSISEV
                     DSNDLWQKAVLGVCVITNEGPFANSILDQVLNYIQTITMCRIFNNKIEIIHL"
     gene            1359..1712
                     /gene="rbfA"
                     /locus_tag="AC159U_05145"
     CDS             1359..1712
                     /gene="rbfA"
                     /locus_tag="AC159U_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3336475.1"
                     /translation="MGFGIKRNARVGVLIKEQLAKLILSGDIKDPRVADCVITGVKMT
                     DDLGLITVYFMSMTKEHKEQDIIKGFNSAKGFIKSKLSKNLRLKKFPEFRFEYDRSFE
                     TGYKVDELIKESLGE"
     gene            1705..2688
                     /locus_tag="AC159U_05150"
     CDS             1705..2688
                     /locus_tag="AC159U_05150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3336476.1"
                     /translation="MNEFKSVAELINSNSNFLIASHENPDEDAIGSTLSLGIALEQLG
                     KNVYLYNETGVTQALKYLPKSKKINNNLDNIPFELDTVIIVDCTDSMRVGKKFDKFIS
                     NLSVLDITIDHHFTKKTKSENCILDQHSPSTGFLIYKLLKELDVKITKDIADNIYSTI
                     VGDTGSFAYSNTTAETFKVAAELLEFGVKPYEISKNIFENEPLNKIKLLGLVLNTLQL
                     SHGGKLATVFITREMFNETSTTKQHTEGLINYPRSVKGVELAVLYRQEEDETKDENWK
                     ISLRAKDGINVAEIAQRFGGGGHESAAGCSIKGELAEVRNILLTEIEKVFK"
     gene            2685..>3505
                     /gene="truB"
                     /locus_tag="AC159U_05155"
     CDS             2685..>3505
                     /gene="truB"
                     /locus_tag="AC159U_05155"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3336477.1"
                     /translation="MNGILILDKPSGITSAKALDKAKKVIGANKAGHTGTLDPFATGV
                     LTVCLNEATKLIPYLNENIKEYEAVLKLGQRTDTLDLTGEVTQSVAKIDVPISEIQRV
                     INCYIGDTEQVPPDFSAVKIEGRRLYKYARKKINVKKPKRKIHIEKIEILDYYNPFIK
                     ISVKCSKGTYIRSLADDIGADLKVFAHLVELKRVKSGVFCINSSYSLDDVQNGEYNLI
                     TMSEAISDIKSLFISKDNTFKIKSGITLRKGDLNSSSITEINRDEVIKVLYENNL"
BASE COUNT         1323 a          469 c          722 g          991 t
ORIGIN      
        1 ttggaattga tattgcttca ggctgatgta ttggagctaa aagcgaatcc tgataaaagg
       61 gcaaatggta tagtagtaga atcaaaactt gataagggca ggggaccatt agctactgta
      121 atagtcaaag aaggtacact caaaattggt gacaacataa tatccggaac agactcgggg
      181 aaagtaaggg cgcttattga tgacagtggt aagaacatga aagaagcagg tccttcaact
      241 ccggctgaaa ttatggggtt ttcctcggtt ccaacagcag gagtaccatt ttatgtagta
      301 catgatgata aagctcttaa ggaaatagtc tcacatagag agagtaaagt tcaaggtaaa
      361 attgcaggtc cggacagaaa aatttcgctc gataatttgt ttgaaaatct tgaacaggga
      421 gaaacaaaag aactagccct aattataaaa gcggacactc agggttcagt aggggcgctt
      481 atagattcta tagagaaatt aagtagtgaa aagtgcagtg taaaagttgt tcactcagct
      541 gtaggtgcta ttaatgaatc cgatatagtt ttagcaggag cttcaaatgc aattatcctg
      601 ggatttaatg taagacctga tcccaaggca attcagctag ctgaaaagca aggtgtgtca
      661 ttagagctcc acagcattat ttatgatgct atagacagga tcaagaatgc tatggaagga
      721 ctgctggagc caattaaaaa agaaaacatt acgggtcatg cagaagtaag agatactttt
      781 catgtttcaa aaattggaac tattgcgggt tgttatgtta ctgatggtaa attttcaaga
      841 aattcaaatg tgcgtctaat aagggatgga attgttattt atgatggaca actcgaatca
      901 ctcagaagat ttaaagatga tgtcaaagaa gtacagaacg gatatgaatg cggtataagt
      961 attcgcaact tcaacgatat aaaagttgga gatatattgg aaaactatat aatagaagaa
     1021 gaaaagcaaa aactataaat tataaatggt agtaggagtt ctcaagatag agcttttgat
     1081 tgatgaaagc agatctctta aagataaacg aaaggtagtt aagagcataa cagagaaggt
     1141 aagaaataag ttcaagaatg tttctatttc agaagtagac tcaaacgatc tgtggcaaaa
     1201 agcagtactt ggcgtttgtg taatcaccaa tgaaggccct tttgcaaatt caattctcga
     1261 tcaggtctta aactatattc aaactattac catgtgcagg atatttaata ataaaatcga
     1321 gataattcat ctgtgaaata tcatacggag tacagacgtt gggatttggt ataaaaagaa
     1381 atgcaagagt tggtgtactt ataaaagaac aactagccaa attgatcctt tcaggagata
     1441 taaaagatcc aagagtagca gactgtgtta ttacgggagt aaaaatgaca gatgatcttg
     1501 ggcttatcac agtgtatttt atgagtatga caaaagaaca taaggagcaa gacattataa
     1561 aaggatttaa tagcgcaaaa ggttttataa aatcaaaatt atctaagaat ttaagactta
     1621 aaaaattccc tgaattcaga tttgaatatg atagatcatt tgaaacggga tataaagtgg
     1681 atgaattaat aaaggaatct cttggtgaat gaatttaaga gtgttgcaga attaattaac
     1741 agtaatagta attttttaat agcatcacat gaaaatcccg atgaagatgc aattggttct
     1801 acactttcac tgggtatagc gctggagcag cttggtaaaa atgtttattt atataatgaa
     1861 accggagtga ctcaagcact aaagtatctg cctaaatcta aaaagataaa taataatctg
     1921 gataatattc catttgaact agatactgtg attattgttg attgtacaga ttcgatgaga
     1981 gtagggaaaa agtttgataa atttatttcc aatctctctg tacttgatat tacaattgat
     2041 caccatttta caaaaaaaac taaatcagaa aattgtattt tggatcagca ttctccatca
     2101 actggatttt taatttataa actattaaaa gaactggatg ttaagatcac aaaagatata
     2161 gctgataaca tatattcgac aattgttggt gatacaggtt cttttgctta ttccaatacc
     2221 acggcagaaa cctttaaagt tgcagctgaa ttattagaat ttggtgtgaa gccttatgaa
     2281 atttcaaaaa atatctttga gaatgaacct ctaaataaaa taaaacttct gggtttggta
     2341 ttaaatactc tgcagcttag tcatggtggc aagttagcaa ctgtgtttat tacaagagaa
     2401 atgtttaatg aaacatcgac aacaaaacag cacaccgaag gtcttataaa ctatccgagg
     2461 tctgttaaag gagtagagct tgccgtactt tacagacaag aggaagatga aacaaaggac
     2521 gaaaactgga aaataagttt aagagctaag gatgggatta atgtagctga aatagcccaa
     2581 aggtttggtg gaggaggtca tgaatctgcc gcaggctgca gtattaaggg agaactggct
     2641 gaagttagaa atattttgtt aacagaaatt gaaaaggtat ttaagtgaat gggattttaa
     2701 tactggacaa accatctggt atcacatcgg caaaagctct tgataaagcc aaaaaggtta
     2761 ttggagctaa taaagcaggg cacacaggaa ctcttgatcc ttttgcaaca ggtgtgctca
     2821 cggtttgttt aaacgaagca acaaagctaa taccctatct caatgagaat ataaaagaat
     2881 atgaagcagt acttaaacta gggcagagga cagacacact tgatttaaca ggagaagtta
     2941 ctcaaagtgt agcaaaaata gatgttccta tatctgaaat acaaagagta ataaattgtt
     3001 atatcggaga taccgagcag gtaccaccag atttctcagc agtaaagata gaaggtagaa
     3061 ggctttataa atatgcaaga aaaaaaatta atgtaaaaaa gccaaaaaga aaaatacata
     3121 tagaaaaaat tgaaatttta gattattata atccgtttat aaagatttct gtaaagtgtt
     3181 cgaagggtac gtatataaga tcacttgcag atgatattgg cgccgactta aaagtgtttg
     3241 cgcatttggt tgaattaaaa agggtaaaat ccggggtttt ttgtattaat agttcttatt
     3301 cactcgatga tgttcaaaac ggtgaataca atttaataac gatgagcgaa gctatatccg
     3361 atattaagtc tttatttatc agtaaagaca acacttttaa gattaaaagt ggtataactt
     3421 taagaaaggg cgatttaaac agctcaagta ttaccgagat aaaccgagat gaggtaataa
     3481 aggttttata tgaaaataat ttaat
//
LOCUS       DBNGSV010000147         3197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1549702, whole genome shotgun sequence.
ACCESSION   DBNGSV010000147 DBNGSV010000000
VERSION     DBNGSV010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1549702"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="AC159U_05160"
     CDS             <1..436
                     /locus_tag="AC159U_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336478.1"
                     /translation="TAYQYSETGRLMGTYKMEHGTGYDIWRHVSMEEDASPDDFYVSE
                     VHSMKDGLPHGYEWWLNEDKTIYVEICSYMGKYHGITREWNFNGKLRRGYPKYYVHNK
                     KVDKRIYIKAYKTDSSLPEFKKEDNLPIRNFPPEIKKILITK"
     gene            517..951
                     /locus_tag="AC159U_05165"
     CDS             517..951
                     /locus_tag="AC159U_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336479.1"
                     /translation="MSQQELDEYNENINKYFEDNTDRIACVLAYSVPGRFKPMVITYH
                     KMFNDEIVRKYNAHVYFANGLAGNEDLLITYFIMAKDEHKDDPQAVNADCKMYVNKFA
                     DYNKEVTGAVAANFYDVGSKEKIEEFRQGEKESLTDIAFFRA"
     gene            1167..1376
                     /locus_tag="AC159U_05170"
     CDS             1167..1376
                     /locus_tag="AC159U_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR3336480.1"
                     /translation="MSERVIGTVKWFNSTKGFGFIERENDKDVFVHYSEISGDGYRSL
                     EEGQKVEFTVVDGQKGPQAQNVVAA"
     gene            1546..2097
                     /locus_tag="AC159U_05175"
     CDS             1546..2097
                     /locus_tag="AC159U_05175"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3336481.1"
                     /translation="MKNSNLTTKQRAMVEMWDKHTAAEFENKSIEATMDTMSADPFVN
                     HVPVMTGGVGTDQVRHFYSTYFIPGHPPDTEVTPVARTVGEDRLVDELIHKFTHTVEM
                     PWILPGVAPTGKLVEFAVIVVVLFEDGKISGERIYWDQASVLAQVGLIDSNKLPVAGA
                     EASRKVLDPNSESSNVLIERVNK"
     gene            complement(2137..2382)
                     /locus_tag="AC159U_05180"
     CDS             complement(2137..2382)
                     /locus_tag="AC159U_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336482.1"
                     /translation="MEIKAGDYVEVTTKDKKQYRFKTRKITEDFIKGENNNILLSDIY
                     KIEKRGDDNIIIYSETSPYLKVLQFLDNIYYFYYLEY"
     gene            complement(2764..>3197)
                     /locus_tag="AC159U_05185"
     CDS             complement(2764..>3197)
                     /locus_tag="AC159U_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold protein"
                     /protein_id="HIR3336483.1"
                     /translation="TMNQILKKHISSLTVLIIFSILAIGTTDTDVDTKKVQSQSPAYT
                     LTANQLYNEYKNNEVAADSKYKGKIVVVSGTVQDIGKDIMNEAYIVIGGTGLLDGVQC
                     MFTKGQQSSVAKLSKGQQVKVKGEVSSLTLGVVLLFRCSVQ"
BASE COUNT         1044 a          529 c          627 g          997 t
ORIGIN      
        1 tacggcatat caatattctg agaccggaag gcttatgggt acctacaaaa tggagcacgg
       61 cacgggttat gatatttgga ggcacgtgag catggaggag gacgcttcac cggatgattt
      121 ttatgtatcc gaagttcata gcatgaaaga cgggcttccg catgggtatg aatggtggct
      181 caacgaggat aagacaattt atgtagaaat atgttcttat atgggcaagt atcacggaat
      241 tacgagggaa tggaatttta atgggaagct tcgcaggggt taccccaaat attatgttca
      301 taacaaaaag gtagataaaa ggatttatat aaaagcctat aaaacagaca gttcacttcc
      361 tgagtttaaa aaagaagaca accttccaat tagaaatttc ccacccgaga ttaaaaagat
      421 attaataaca aaataaaagc aatcagacac tcgcataata tttaatccat agtattattg
      481 taagcttcaa aattaaaagc tttaaagagg aaactaatgt cacaacaaga actcgatgaa
      541 tataacgaaa acataaataa atattttgaa gataatacgg acaggattgc gtgtgtgctg
      601 gcttattctg ttcccgggcg ttttaagcct atggtgatta catatcacaa gatgttcaat
      661 gatgagattg taagaaagta taatgcccat gtgtactttg caaatggact tgccggaaat
      721 gaggatttgc ttatcacata ttttattatg gcaaaagacg aacataaaga tgacccgcag
      781 gcagtaaacg cagactgcaa gatgtatgta aacaaatttg ccgattataa taaagaggtc
      841 acgggtgcag ttgctgcaaa cttttatgat gtgggatcaa aggaaaaaat agaagagttc
      901 aggcagggtg aaaaggaaag tctcacagac attgcatttt ttagagcctg atactgccaa
      961 aattgcaccc ggtgagttat ttaactaata tccactaatg tagtaaaaaa gattatttgt
     1021 tattgacata aagttcaaat agtgcaaact tttattagtt gttgcagtag ttctgtctga
     1081 ttaaagattt actctttaaa gtagtatctg taattagctt tgaaccacta gcattaacca
     1141 gtttaattta tggaggttac ctggacatgt cagagcgtgt aattggcact gtaaagtggt
     1201 tcaattctac caagggtttt ggttttattg agcgcgaaaa cgacaaagac gtttttgttc
     1261 actacagcga gatcagcggc gacggctatc gttctttaga agaaggccag aaagttgagt
     1321 tcacagtagt agacggacaa aaaggtccac aagcacagaa cgtagtagct gcttagtgta
     1381 taaaaattaa agtgcgtcga tatatttcgg cgcacttcct ttctcaattc tttcttaatt
     1441 ttatttactc tttaagattc aggtttttta tattatcatt ccatctataa ctctattttc
     1501 agattgatat atatcggctg atacaggcag gaggattata ttattttgaa aaattctaat
     1561 ctaactacca aacaacgggc aatggtagag atgtgggaca aacacacagc tgccgagttt
     1621 gaaaataaga gcatagaggc cacgatggat accatgtcag ctgacccctt tgtaaaccat
     1681 gtacccgtga tgacaggtgg agtgggcaca gatcaggttc gccatttcta cagcacttac
     1741 ttcattcctg gtcatccacc cgataccgag gttactccgg ttgcccgcac tgtgggtgag
     1801 gacagattag tggatgagct tattcataaa tttacacaca cagtagagat gccgtggata
     1861 ctcccgggcg ttgcacctac aggaaaactt gtagaatttg cggtaatagt ggttgtgcta
     1921 tttgaagacg gaaagatttc aggcgaaaga atatactggg atcaggcctc ggtacttgca
     1981 caggttgggc taatagattc taataaacta cccgttgcag gagcagaggc ttctcgtaaa
     2041 gttcttgacc cgaattcgga gtcgtcaaat gttttgattg aaagggtcaa taagtagtta
     2101 attaaaaatt ggtaaaattt tctgtaaagt ttttgactag tattctaaat agtaaaagta
     2161 gtatatgttg tcgaggaatt gcagtacttt tagatatggt gaggtttctg aatatattat
     2221 aatattatca tcgcctcttt tttcaatttt atatatatca gaaagtagga tattgttgtt
     2281 ttcaccttta ataaaatctt cagttatttt ccgggtttta aacctgtatt gctttttatc
     2341 tttagtagtt acttccacat aatccccagc cttaatttcc atttgtttac tgatatcttc
     2401 tacatacaaa actctgtatt taggtccgca tgacacagaa aaaaacaagt tattgtaaga
     2461 ataaggattg ttaaaattgt tttcattaaa aacttatttt actctttaat ttccaaatga
     2521 aaaatatcgg gtcttgtact atctaaaaaa atcccaaatg tattacaaaa ctaaaatccg
     2581 gcagcgccgg cttatctgat taataagagt aaaggtctta agcaggtggg agtttgattg
     2641 atattatgta gatggataat atttagggaa aattactatt taatactctt aatcctcaaa
     2701 ttaagaacat ctaatttaga gaatgatgtt gtctaaacat aaaacagatc acacgaatgt
     2761 aatttactgt acactgcatc gaaatagcaa tacaacacct aaagtcaagc tagaaacttc
     2821 acccttaact tttacttgtt gacctttaga cagctttgct acagaagatt gctggccttt
     2881 tgtgaacata cattgtacac cgtccaataa accggtaccc ccaattacaa tatatgcttc
     2941 attcataata tcttttccta tatcttgaac tgtcccagaa actacaacta ttttgccctt
     3001 gtactttgaa tcggctgcaa cctcattgtt tttgtattca ttgtaaagct ggttagccgt
     3061 aagagtgtat gcaggagatt gtgattgaac ttttttagta tctacatctg tgtcagtagt
     3121 cccaatagca aggatactaa atataattaa aacggttaat gatgatatgt gctttttaag
     3181 tatttgattc attgttt
//
LOCUS       DBNGSV010000148         2607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1619202, whole genome shotgun sequence.
ACCESSION   DBNGSV010000148 DBNGSV010000000
VERSION     DBNGSV010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1619202"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..565)
                     /locus_tag="AC159U_05190"
     CDS             complement(392..565)
                     /locus_tag="AC159U_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin-like domain-containing protein"
                     /protein_id="HIR3336484.1"
                     /translation="MCPKCNQTYWKCSKCGFLITASMPPDICSQCHEKCDFLNVTCYT
                     PECGGPGHIDPRL"
     gene            complement(779..1519)
                     /locus_tag="AC159U_05195"
     CDS             complement(779..1519)
                     /locus_tag="AC159U_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR3336485.1"
                     /translation="MLRYLTRREILKKITNYLMAFFMMPFFSIKKSMAVEADGTTGSM
                     ERHMNLPKPLLKGEVSVEEAIKHRRTIRSYLSKALTLEQLSQIFWAAQGITEDGGYKR
                     SAPSAGALYPLDIYAAVGDNGVEGVKAGIYHYNAHKHVALLIAEGDFRKELARTALSQ
                     MWMARAPINLVITSEYSRITSKYGTRGEMYAMIEAGHVGQNIFLQAEALGLRAGIVGA
                     FHGNDVIRVMKISRSHDPLLILPVGYGA"
     gene            complement(1637..1993)
                     /locus_tag="AC159U_05200"
     CDS             complement(1637..1993)
                     /locus_tag="AC159U_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR3336486.1"
                     /translation="MVFRSLLTFIVVLSLIIGCAVPKIVGGLILKDSIKDGIYDGEAR
                     VEPVKVLARVTIQNQRVTNIGLIEHRNWKGKAAENIIPRRIIDEQSTKVDAVSGATVG
                     STAIMNAVNDAIQMAK"
     gene            2211..>2607
                     /locus_tag="AC159U_05205"
     CDS             2211..>2607
                     /locus_tag="AC159U_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336487.1"
                     /translation="MRPTPPEREPSATDPALAKWASPRRTIIDLRQNDWKTPGGTRPT
                     NAFCIGSARLLPQVPFPGVHNRHVLSQIEWWHYSVDYSSGFGQQFVPNPRKHHPWENG
                     AGDEHPARLHTSQVRQNPHDCLKDPGYYKL"
BASE COUNT          713 a          627 c          512 g          755 t
ORIGIN      
        1 ctcgtagacg cctaattgaa atgaccgata aaaatatgag atgttttttc cggaattttt
       61 ggagtcattg atagcttctc cgatatggta tcgaaggtag aagataccat aagtgaagcc
      121 tccccaccct aaaggttgga ggttctgcct tacggcagta tttcgtgccc gtgtaagtat
      181 ctcagctcat aatatatagc taccttgacc ccattctaaa ggacggagcg gcttgcgggg
      241 agaatgcgaa caaatgatct cagggtaatg tagggtcaca cacggcatgc aatatcattc
      301 cccgactcat atcggctaaa cagcgaagct tctaataaat agagcggcat gtccacagga
      361 gtgaatcatc aagatgtaac tggattcttt cttataatct tggatcaata tgtccggggc
      421 ctccgcattc cggtgtatag caagtaacat taagaaaatc gcacttttca tggcattgtg
      481 agcatatatc cggcggcatt gaagccgtga tgaggaatcc acatttagaa catttccaat
      541 aagtttgatt gcatttagga cagatatctc ctttgaattt tccatcagca gtatatccgc
      601 agtgtgcaca cgcatattct tcttgtttgc ttcccatttt ctttacctcc ttttacaatt
      661 tggtataaga ttcacctttt acgactatat ttccgaaaaa atatcattcc gtcatgaata
      721 aaatccaccc ggttcttcag ttgctcccac acttgatttt ttattcaagc ccagggcttt
      781 acgccccata tccaacaggc aaaatgagca gggggtcatg ggacctgctg attttcatga
      841 cccgaatcac atcattgcca tgaaaagcac ccacgatccc ggctctcaga ccaagggcct
      901 ccgcctggag aaagatgttt tgtcctacat gaccggcctc gatcatagca tacatttctc
      961 ccctggtacc gtacttgctt gtaatccggc tatattctga ggtgattacc aggtttatgg
     1021 gagctctggc catccacatc tgcgagagcg ctgtcctggc caactccttt ctgaaatccc
     1081 cttctgcaat caacaatgca acatgtttat gagcattata atgatagata cccgccttta
     1141 ctccttccac gccattatca cccactgccg catagatatc caaagggtat aaagccccgg
     1201 cagacggagc cgaccgtttg tagcccccat cctcggtgat accctgggca gcccagaaaa
     1261 tctgtgaaag ctgttcaagg gttaaagctt ttgatagata tgaccgaatg gttctacgat
     1321 gtttaatggc ctcctctaca gagacctctc cttttaataa gggcttaggt aagttcatgt
     1381 gacgctccat gctcccggtc gtgccatctg cctccacggc catggacttt ttgatagaaa
     1441 aaaaaggcat cataaaaaat gccatgagat aattggtaat cttttttaaa atctctcttc
     1501 ttgtaagata tcttagcatg cggtgtcttt tgatactaaa tggagtagga acttgaattg
     1561 tgaattataa aggcatctta ttcacttttg gcaacagccc gtcgagcttt tacactgttc
     1621 ccctcccttg tttaacttat tttgccattt gtatggcatc atttactgcg ttcataatgg
     1681 ctgtgctacc aacagtagct cctgaaacag catcgacctt tgtggattgt tcatcaatta
     1741 ttctacgagg aataatattt tctgctgctt tgcctttcca attacgatgt tctatcaaac
     1801 caatatttgt gactcgttga ttttgaatcg taactctggc taaaaccttt accggttcaa
     1861 cccttgcttc accatcatat atgccgtctt taatactatc ttttaagatc aggcctccaa
     1921 cgattttcgg aacagcacat ccaataatta gtgacaatac tactataaac gttaataagc
     1981 ttctaaagac catatttttc cactagaatt tacaggtgga gaggctcgct caaatctatt
     2041 tccaaagcac ataaagtttt caaatgccaa ccaactcgtt ttacttactg ttgtggtaat
     2101 acaggagaaa tataaattta acttgtaccg cagatcttgc cgtaattctc aggaacccat
     2161 cgatacgata agatcccgat aaagttcagc tgcccattgg acttggttaa atgagaccga
     2221 ctcctccgga gcgtgagcca tctgcaactg acccggccct agcaaaatgg gcttcacccc
     2281 ggagaaccat aatcgatttg cgtcagaatg actggaaaac gcctgggggg accaggccaa
     2341 cgaacgcttt ttgtataggt tctgcaaggc ttctaccaca ggtccctttt ccgggagttc
     2401 ataaccgtca tgtactgtca caaatcgaat ggtggcatta ttctgtggat tattcttccg
     2461 gattcggtca acaatttgtt ccaaatccac ggaaacatca cccatgggag aatggggcgg
     2521 gagatgaaca tccagccaga cttcacacca gtcaggtacg gcaaaaccca catgactgct
     2581 taaaagatcc cggatattat aaactcc
//
LOCUS       DBNGSV010000149         9695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1342265, whole genome shotgun sequence.
ACCESSION   DBNGSV010000149 DBNGSV010000000
VERSION     DBNGSV010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1342265"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..957)
                     /locus_tag="AC159U_05210"
     CDS             complement(265..957)
                     /locus_tag="AC159U_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3336488.1"
                     /translation="MSREKNKEDGFTLIELLVVAAILGILLAVAIPNLIKARISANEA
                     NARKSLQTLRDAEYEFFEGDLDNDGVKNFTSSIGSLLTSGSLRCPATFGATECTEFDS
                     LIDSRFEEMVSVSGTFADCTVPSAGYCIKFDGTIGTDATSPGGSEDPTGGFDDFGQGG
                     FGFPPDPDPIPDLSLDTDFGWLASPAGVNKTGHKDFATYADTRAIHCVISSLPSGHAG
                     EFEARSTSPVCY"
     gene            1120..2442
                     /locus_tag="AC159U_05215"
     CDS             1120..2442
                     /locus_tag="AC159U_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3336489.1"
                     /translation="MIEPLRYPAYRHLFSAQIIALLGTGLTTVALALLTYDLAGNKAG
                     TVLGTALALKMIAYVSIAPVVGGLAGGVNRKRVLVILDLIRAGFVMCLPFVSEIWHIY
                     LIIFLMQSCSAGFTPMFQSTIPDIIPDEKTYTKALSLSRLAYDLESLLSPALAAAALL
                     FMSFDALFAMNSAAFLVSAILVVTAVLPAAKKKESEHVLSTWQKITFGTRAYLATPRL
                     RGLLALSMAVAAAGAMVIVNTVVIIRSSFGMGERHVAYAFAAYGAGSMIAAFIIPKVL
                     NKFADRPVMLTGAVLLATGLFTGTAANTFTWLMIVWFLLGIASSIINTPTGRLLRRSS
                     SETDRPAYFSAHFSLSHACWLITYPLAGWLGISIGIINTYYLFGGIVVLGFLSALIFW
                     PSKEAYSIKHIHKDLNPDHPHLEDAKAVDGGYEHTHDYVIDYHHKTWP"
     gene            2529..3047
                     /gene="tpx"
                     /locus_tag="AC159U_05220"
     CDS             2529..3047
                     /gene="tpx"
                     /locus_tag="AC159U_05220"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIR3336490.1"
                     /translation="MSEIRKNAITLRGNAVSLVGPELKAGDNAPDFTVYEALDKPLTL
                     SDLGDKVKIFNVIVSVDTPVCDKQTRLFNEKAAELGDGVEIVTLSVDLPFALSRYCGA
                     AGIDKIKSASDYKDLSFGPAYGVLIDEFKLLCRAIFVLDSNNIVQYAEYVSEVSEEPD
                     YDSALEAAKSLI"
     gene            3072..3620
                     /locus_tag="AC159U_05225"
     CDS             3072..3620
                     /locus_tag="AC159U_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR3336491.1"
                     /translation="MGIKDKITKIFSSPGSGDDKSVSARNKATIHGPVHHGHVDIPET
                     EEVSDLKTENISSHSDESEDESEGDKPQMNEENNKSAGTELSEAKIYDVLSNVYDPEI
                     PIDIVNLGLIYGIDIVGDKVEVKMTMTSPGCPASVQITSESKYLIEELEGVTEANIEI
                     VWDPPWDPSRMSEEARQSMGFT"
     gene            3705..4160
                     /locus_tag="AC159U_05230"
     CDS             3705..4160
                     /locus_tag="AC159U_05230"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3336492.1"
                     /translation="MSKLSIGDKAPHFEAQSYEGKTISLSDYAGKSIVILYFYPRDNT
                     PGCTKEACSMRDGMDELSGLGIQVLGVSTDSVKSHEKFRDKFKLNFPLLSDKSKEIVK
                     AYDVQSAFGSASRKSFLIDKNGIIRHIWNKVKTSDHANEVMDKVKELGL"
     gene            4222..4962
                     /gene="bioD"
                     /locus_tag="AC159U_05235"
     CDS             4222..4962
                     /gene="bioD"
                     /locus_tag="AC159U_05235"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR3336493.1"
                     /translation="MSLPSKIFVTGTDTGVGKTVVTAALAHCLSEAGKRVSVVKPVQT
                     GTSCQKVSDIQFVYKLLGRDINYTDHCPYSFSQPLSPKTGAELENSCIDIEIIKSAIN
                     KQARLNDIVIIEGAGGLMVPITERFSMADLAKDTGCKLIVTARPGLGTINHTALSIEY
                     AKSRGLQILGIVINNFPKEPGLSEKTNPQELIRITGVPVVGVLKNDTAIDVENGHLGM
                     LKNNSVNSFISQFGGNLEIDEFLSSIKL"
     gene            5112..5687
                     /locus_tag="AC159U_05240"
     CDS             5112..5687
                     /locus_tag="AC159U_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3336494.1"
                     /translation="MKKGFKDGMAAILLVAAITIVSVISNRDAQSESEGPTQPIFFSH
                     KIHAGENQIPCEYCHSYVAVSAKPGIPSVQKCMGCHTHIPGKDELYEDGPEKINFQTE
                     INKLKDYWDKKTPIPWVKVHYVAEHARFKHKPHIKRGIECSACHGEVENMHVVKRVKK
                     IEMGWCITCHEDNAKDEKELERLKDCLTCHY"
     gene            5702..8707
                     /locus_tag="AC159U_05245"
     CDS             5702..8707
                     /locus_tag="AC159U_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3336495.1"
                     /translation="MSKDLIKQTEDKNGLKRRDFLKLVGVVGTGAAVSGCWSEPADEL
                     IPYVIPPDNVIPGIPNWYASTCTECPAGCGVIVKNMDGRAIKVEGNPENPVNSGSLCA
                     RGHASLQGQYNPDRFRSPLYKNEAGEMEPLSWDEAETKFAEIIKELTDSGNADKIVFL
                     TNNVSGTLNSLISDFLAAIGGGKHISYEPVSYESLKEANRITFGINEIPTYNIDKADF
                     VVSFGADFLETWLSPVSYTKQFSHMHGYDHGKMGKFVYVSPRSALTGANADSWLAIKP
                     GTEMVLALGLVREIIYSGLSNAPDTGVLRGIVSRYTLEMVSSKTDIAADTIKQLARDF
                     AKAESSLAVGGSLTNATDNATKTIAAINLLNYVSGNIGKTINFNASQSIGDVATYREI
                     EELVQSMNDGKVELLVTYDTNPVYSVPKVLGFEQALEKVSHVVSLSSQPTETTTKTNL
                     VLPDNTTLEKWGDYFPARGVYGLIQPAMKPIFNTKAIGDTLLSVSKKLELTYENLNHI
                     NYLSYLQNSWRKIHSANQSFRLFEDFWRASQQAGGYYADYSASGVSLSPSVQRIRFTE
                     SRIDNKSGNLYFVTYPSLSLYDGRGANRPWLQELPEPLSTSVWDSWAEIHPDTASSLG
                     VSEGDYITIETEQGSITTQAFVFKGIKEDTVAVALGQGHTSIGQFGDDKGVNVLDILP
                     NKKDINSGAFAWLSTKANVSKSGDSDKLVKVQMTFSQEDRHIAQATTVSALAHSGDGH
                     GDSHGGGHGNGGDSHGGGHGKLNMYPEREFQDNHWGMSIDLSKCVGCGSCVTACNAEN
                     NVSFVGKDQVSRGRYMGWIRIERFFDDGDEFKTKFIPMLCQHCDNAPCEPVCPVYATY
                     NNYEGLNVMVYNRCVGTRYCANNCSYKVRRFNWYDYELPEPLHMQLNPDVTVREKGIM
                     EKCSFCSQRIIAAKDKVKDENRKIKDGDVKPACVQTCPAEAITFGNLKDHDSKVYKKT
                     QDKRSYHVLEELNTMPTVSYLKKVEWDKA"
     gene            8707..>9695
                     /gene="nrfD"
                     /locus_tag="AC159U_05250"
     CDS             8707..>9695
                     /gene="nrfD"
                     /locus_tag="AC159U_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3336496.1"
                     /translation="MSNETTTTPLTYSRVNDDIIRMLEKPSAKWLALFIFDLSILGIG
                     AMCFIYQVYVGLGVAGYSHPVFWGVYITNFVFWVGIAHSGTLISAILFLFRARFRMSI
                     YRISEAMTVFAVATAGLFPIIHLGRPWNFYWLMPYPNQRELWINFKSPLIWDVFAVST
                     YATVSTVFFIIGMIPDIAAIRDRVVEKPRKIIYTLLSLGWKGSNWEWLHYTRAYLYFA
                     AFAAPLVISVHSVVSWDFAMANTPGWHTTIFPPYFVAGAIFSGLAMVITLCIPIRKIF
                     HLEDYITMDNFDAMAKMIILTSGIVGYAYGAEFFMAWYSGNPHEWGQFLYRATG"
BASE COUNT         3042 a         1801 c         2084 g         2768 t
ORIGIN      
        1 cttaaatatc ttattatctg ctctggtgtg taacgctttc tgggcatttc ccttgcctcc
       61 tattcatagg gtttattacc taaaattcta acttataatc tggtactatt atatggggca
      121 aggtcaagat gtaatatttc ttatatcaga aaacaaatgt cttaaggacg taagatatat
      181 agcaagtgga agcaaactat atctgatatg tttatctata aaactattga taaattagta
      241 actacggaaa tattattata ttaatcaata acaaaccggg gaagttgatc tggcttcaaa
      301 ttcaccagcg tgaccacttg gtagtgaact tataacacag tgaatagccc ttgtatccgc
      361 ataagttgca aagtccttat gacctgtttt gttgacacct gcaggtgaag cgagccatcc
      421 aaaatcagtg tcaagtgaga ggtcgggaat tggatccgga tcaggtggga atccaaagcc
      481 tccctgccca aaatcatcaa aaccaccagt aggatcttct gaacctccag gactagttgc
      541 gtcagtgcct attgtgccat caaactttat acagtaacca gctgaaggaa cggtgcaatc
      601 tgcaaaggtt ccagatactg ataccatttc ttcaaatcta ctatcaatta atgagtcaaa
      661 ctctgtacat tcagtggctc caaaggttgc agggcacctg agagaaccgg atgttaacag
      721 ggacccgatt gaacttgtaa agttctttac tccatcatta tccaaatcac cctcgaagaa
      781 ttcgtattct gcatctctta gggtttgtaa ggattttctt gcatttgctt catttgcaga
      841 aatacgagct ttaattaagt ttggaatagc aactgcaagc agtattccca aaattgcagc
      901 aactactaaa agttcaatta gagtgaatcc atcttcttta ttcttttctc tcgacatttt
      961 atgcccccgt ccttaatcag aaattataaa ataaaagtaa aaaaaatata gatatatttg
     1021 aatttcaata caaaagtatt tagtaatgaa atatacttct tacattgtaa ctaactcaat
     1081 gtatcttgac ctcgtgatgt tattactcaa aatactaata tgatagaacc actaagatat
     1141 cctgcgtaca gacatctgtt ctcagctcag ataattgcac ttctgggtac gggccttacc
     1201 actgttgcac tcgctcttct aacttatgat ttagctggaa ataaagccgg cactgtgctt
     1261 ggcactgctc ttgcgcttaa gatgattgca tatgtatcga ttgcacctgt agtagggggg
     1321 cttgcaggtg gagtaaacag gaaaagagtc ctggttatac ttgatctcat acgtgcaggg
     1381 tttgtaatgt gtctgccttt tgtgagtgaa atatggcata tctatttaat aatatttctt
     1441 atgcagtcct gctctgcggg atttactcct atgtttcagt caacaattcc cgatataatc
     1501 cctgatgaaa aaacatatac caaggcgcta tcactttctc gtcttgcata tgacctcgaa
     1561 tcacttttaa gccccgcact tgcagcggca gcgctgcttt ttatgagctt tgatgctctt
     1621 tttgcaatga actcagcagc atttcttgta tcagcaattc tggtggttac agctgtattg
     1681 ccggctgcta aaaagaaaga gtcagagcat gttttatcaa catggcagaa aataactttt
     1741 ggcaccaggg cttatcttgc tacgccaaga ctcagaggct tgcttgcgtt gtctatggca
     1801 gttgctgcgg ctggagccat ggtaattgta aacactgtag ttataatacg aagtagtttt
     1861 ggaatgggtg agcgccatgt tgcatatgca tttgcagcct atggggcagg atctatgatc
     1921 gcagcattta ttattccaaa agtgcttaat aaatttgcag acagaccggt gatgctcacg
     1981 ggggcagtct tgctggcaac gggattgttt acaggaacag cagcaaatac atttacatgg
     2041 cttatgattg tatggttttt actggggatt gcttcatcaa taataaatac acccactgga
     2101 agattgctca ggcgttcatc ttctgaaaca gacagaccgg cttatttctc tgctcatttt
     2161 tccctttccc atgcatgctg gctcatcact tatccccttg caggatggct tggaatatcg
     2221 attggcataa ttaatactta ctacttattt ggaggtatag ttgttttagg ttttctatct
     2281 gcacttatct tctggccttc aaaagaggca tattctataa aacatatcca taaagatctc
     2341 aatcccgatc atccacactt ggaggacgcc aaagctgtgg acgggggtta tgagcatact
     2401 cacgactacg tgattgatta tcatcacaag acatggccgt agatactatc aaatttaatt
     2461 aatatcattt tacttttgca ctgcatataa tattatctaa agttatacta tttcagcgga
     2521 ggtatgagat gtcagaaatc agaaaaaacg caatcacact tagaggcaat gcagtttcac
     2581 ttgtaggccc tgaactgaaa gcaggagaca atgcacccga ttttacagta tacgaagccc
     2641 ttgataagcc gcttacgctt tcagatcttg gagataaagt taaaatattc aatgtcatag
     2701 tatcagtgga tactcctgta tgtgacaagc agacaagact atttaacgaa aaggcagctg
     2761 agcttggtga cggggttgaa attgtaacac taagcgttga tcttcctttt gctttaagcc
     2821 gttactgtgg cgctgcggga atagacaaga ttaaatcagc gtcggactac aaagacctgt
     2881 ccttcgggcc cgcatacggc gtactaatag atgaattcaa acttctttgc agggccatat
     2941 ttgtattaga tagcaataat atagtacaat atgcagagta tgtaagtgaa gtatctgaag
     3001 agcctgatta tgacagtgct ttagaagcag caaagagtct tatatagagc accttctagg
     3061 attaaaggct tatggggata aaagataaaa taacaaagat atttagcagt cccgggagcg
     3121 gtgatgataa aagtgtaagt gcacggaata aagctacgat tcatggacct gtgcatcatg
     3181 gtcatgtaga tattccagag actgaagaag tgtctgatct gaaaactgaa aatataagct
     3241 cgcattcaga tgaatcggaa gatgaaagtg aaggtgataa accacaaatg aatgaagaaa
     3301 ataataaatc agcaggtaca gagttatctg aggcaaagat atatgacgtg ctatcgaatg
     3361 tttacgaccc tgagattcca atagatatag taaatctggg tctaatatac ggcatcgata
     3421 tagtagggga taaggttgaa gtaaaaatga ccatgacctc gcctggatgt ccagcatctg
     3481 tacaaataac atcagagtct aaatatctta ttgaagaact agagggtgtt actgaagcca
     3541 atatagaaat tgtttgggac ccgccctggg atcccagcag gatgagcgag gaagccaggc
     3601 agagcatggg ttttacctga tataatattt gccattttat tccaccattt tgcaatcttt
     3661 gggttatact tgcctaaaaa acaataaaga ggtcatttgc aactatgtca aaactttcga
     3721 ttggtgataa agcaccgcat tttgaagcgc aaagttatga aggaaaaaca ataagcttaa
     3781 gcgattatgc cggcaagtct atcgtaattc tctatttcta cccaagagac aataccccgg
     3841 gatgtaccaa ggaagcatgt tctatgagag acgggatgga tgaattgtcg ggtctcggaa
     3901 tacaggtgct tggggtcagc acagacagcg ttaaatctca tgaaaagttc agggacaaat
     3961 tcaaacttaa ttttcctcta ttgtcagata aatcaaaaga gatagtaaaa gcctacgatg
     4021 tgcagagtgc atttggttct gcatccagaa agtctttcct tatagataaa aatggaataa
     4081 taaggcacat atggaataag gttaagacct ctgatcacgc taacgaggtc atggataaag
     4141 taaaagagct tggcttataa gctaatttac cttaatccat ctttgggaat tatttcttcc
     4201 tttaggtagt attcattgat tatgagtctg ccaagtaaaa tatttgttac cggtacggat
     4261 actggtgtag gaaagacagt agttacagca gctcttgcac actgtttatc tgaggctgga
     4321 aaaagggtat cagtagtaaa acctgtacag actggcacat catgtcagaa agtatcagat
     4381 atacaattcg tatataaatt acttggtagg gatattaact atacagatca ctgcccttat
     4441 agtttctctc agccgttatc tccaaaaaca ggcgcagaac ttgaaaacag ttgtattgat
     4501 atagaaatta tcaaatcagc tataaataag caggccaggt taaatgatat agtaataatt
     4561 gagggagccg gagggcttat ggtgcctatt actgagagat tttctatggc cgatcttgca
     4621 aaagatacag gctgcaagct tatagtaact gctcgtccgg gtcttggaac aattaatcat
     4681 acagctctaa gtatagagta tgcaaaaagc agaggtcttc aaattttggg tatagtaata
     4741 aacaattttc caaaagagcc gggtttgtct gaaaaaacca atccacagga attaataaga
     4801 atcacaggag tgccggtggt tggagtatta aaaaatgaca cagctattga cgtagaaaat
     4861 ggccatctcg gtatgctaaa aaataattca gttaacagct ttatttctca atttggagga
     4921 aacctggaaa tagatgaatt cttaagttcc attaaactgt aatatttata aaacacagtg
     4981 cagcataggg atttatttgg taaataaata aattattgat taaacatctt gacagagaaa
     5041 ctgattatga catactacgc ttttttttca cagtataaat caatccaata ttttcaataa
     5101 atatttataa tatgaaaaaa ggttttaagg atggaatggc ggcaatacta ctcgttgctg
     5161 caataacaat agtatcggta atatctaacc gagatgcgca aagtgagtct gaagggccaa
     5221 cacagccgat ttttttcagt cacaaaatcc acgccggtga aaatcaaatt ccatgtgagt
     5281 actgtcacag ctatgtagca gtatcagcca aacccggaat cccatctgtt cagaaatgta
     5341 tgggttgtca tactcatatc cccggaaaag atgagctcta tgaagatgga cctgaaaaga
     5401 tcaacttcca gacagaaata aataaattaa aagattactg ggataaaaag accccaatac
     5461 catgggttaa agtacactat gttgccgagc acgctcgttt caaacataaa cctcatataa
     5521 aaaggggcat cgagtgcagc gcatgccacg gtgaagtaga aaacatgcat gtggttaaaa
     5581 gagttaaaaa gatcgagatg ggttggtgta tcacatgcca tgaggataac gcaaaggatg
     5641 aaaaagagct tgaaagacta aaagattgtt tgacctgcca ttactaggat ggagagaata
     5701 gatgtctaaa gatttaataa aacaaaccga agataaaaac gggcttaaaa gaagggattt
     5761 tttaaagctt gtaggtgtag ttggcacagg tgccgcagta tcaggctgtt ggtcagagcc
     5821 tgctgatgag cttataccct atgttattcc tccagacaat gtaatccccg gtatacctaa
     5881 ctggtatgca agcacttgta cagagtgtcc tgcaggctgt ggcgtaatag taaaaaatat
     5941 ggacggaagg gccattaagg tagaaggaaa tcctgagaat cctgtaaact caggaagtct
     6001 ttgcgcaagg ggacatgcct cacttcaggg gcagtacaat cccgaccgtt tcagaagccc
     6061 tctttacaag aatgaagcag gtgaaatgga gccgcttagc tgggatgagg cagagactaa
     6121 atttgctgaa ataataaaag agctaacaga ctctggaaat gcagacaaga tagtatttct
     6181 tacgaacaat gtatccggca ctctgaatag cctcattagc gattttctag cagccattgg
     6241 cggcggaaaa catatttctt atgagcctgt ttcttatgaa tcactaaaag aagcaaacag
     6301 aattaccttt ggaattaatg aaatacctac atataatatc gataaggcag actttgtagt
     6361 ctcttttggc gcagactttt tagaaacatg gctgtccccg gtaagctaca ccaagcagtt
     6421 ttctcatatg catggttatg atcacggtaa gatgggtaag tttgtatatg tatctccccg
     6481 ctcggctctt accggcgcaa atgcagacag ctggcttgca ataaaaccag gtactgagat
     6541 ggttcttgcg cttggtcttg taagagaaat aatctattca ggcctatcga atgctcctga
     6601 tacgggtgtt ctaagaggga ttgtttcaag atacacactg gaaatggttt cctctaaaac
     6661 cgatattgct gctgatacga taaaacagct tgcaagagac tttgctaagg cagaatcaag
     6721 ccttgcagtc ggcggatcac ttacaaatgc tacggataac gctacaaaaa caatcgctgc
     6781 tataaactta ctgaactatg tttcaggaaa cataggcaaa acaattaatt ttaatgcttc
     6841 gcagtccata ggcgatgtag ctacataccg ggaaattgaa gagctcgtgc agtcaatgaa
     6901 tgatgggaag gttgagctgc ttgttactta tgatacaaac cctgtttatt cagttcccaa
     6961 agtgcttgga tttgagcagg cactggaaaa agtatcacat gtggtttcat tatcatcaca
     7021 accaaccgaa accactacaa aaactaatct tgttcttccc gacaatacta cacttgagaa
     7081 gtggggtgat tatttcccgg caaggggcgt ttatggtctc attcagccgg caatgaagcc
     7141 aatattcaat actaaagcca taggtgatac tcttctatcg gtatctaaaa agcttgagct
     7201 tacatatgaa aacttaaatc atataaacta cctatcttat ctacaaaatt catggagaaa
     7261 aattcactca gctaaccagt cattcaggct gtttgaagat ttctggagag catctcaaca
     7321 ggcaggcggc tattatgcag attattccgc aagtggtgtt tctctttctc cttcagtaca
     7381 gagaataagg tttactgaat ccagaattga taataaatca ggcaatctct attttgttac
     7441 ttatccctct cttagtcttt atgacggaag aggagcaaac agaccctggc ttcaagaact
     7501 tcctgagccg ctttctacat cagtatggga ttcgtgggca gagattcatc ctgacacggc
     7561 aagttcactc ggagttagcg aaggtgacta tattacaatt gaaacagaac agggatctat
     7621 taccactcag gcatttgtat ttaaaggcat aaaagaggac actgttgcag ttgcattggg
     7681 acaaggtcat acatcaatag gacagtttgg cgatgataag ggagtaaatg tgctggatat
     7741 tctgcctaat aaaaaagata taaattctgg agcctttgca tggctatcaa caaaggcaaa
     7801 tgtgtccaaa tcaggggata gtgacaagct tgtaaaagtt cagatgacct tttctcagga
     7861 agacaggcac attgcgcagg ctacaacagt aagcgctctg gcacatagtg gcgatggtca
     7921 tggtgactct cacggcgggg gtcatggaaa tggtggtgat agtcacgggg gcggccacgg
     7981 aaaactgaat atgtatcccg agagagaatt tcaggacaac cactggggta tgagtattga
     8041 tctttccaag tgtgttggat gcggatcatg cgtaactgca tgtaatgctg aaaacaatgt
     8101 ttcatttgtc ggaaaggatc aggtatccag aggaaggtat atgggctgga taagaatcga
     8161 aaggttcttt gatgacggcg atgagtttaa aacaaaattt ataccaatgc tctgtcagca
     8221 ctgtgacaat gccccgtgtg agcctgtatg ccccgtatat gctacttata acaactatga
     8281 aggtcttaac gtgatggttt ataaccgctg cgtggggaca agatactgtg caaacaactg
     8341 ctcatataag gtcagaaggt ttaactggta tgactatgaa ctgccggagc cgcttcatat
     8401 gcagctaaat cccgatgtta ctgtaagaga gaaaggtata atggaaaaat gcagcttctg
     8461 ttcacagaga attattgctg caaaagataa ggttaaagac gagaacagaa agattaagga
     8521 cggagatgtt aagcctgcat gcgtgcagac ctgcccggca gaggcaatta catttggaaa
     8581 tctaaaagac catgacagta aggtctataa aaaaacacaa gataaaagaa gttatcatgt
     8641 acttgaagaa ttaaatacta tgccaacggt tagctatctt aaaaaagttg agtgggataa
     8701 agcataatga gcaacgaaac tactactacg ccgcttacat actcaagagt taatgacgat
     8761 ataataagga tgcttgaaaa gccttctgca aagtggcttg cactatttat attcgatctt
     8821 tcaattttgg gaataggcgc tatgtgcttt atttaccagg tttacgtagg tcttggtgta
     8881 gctggttact ctcacccagt gttttgggga gtttatatta caaactttgt gttctgggta
     8941 ggtattgcac actcagggac tctaatttcg gcaatattat ttctgtttcg ggcccggttt
     9001 aggatgtcta tttacaggat atccgaggca atgaccgtat ttgcggttgc cacagctggt
     9061 cttttcccca ttattcacct tggaaggcca tggaactttt actggcttat gccttatcca
     9121 aaccaaagag agctttggat taactttaaa tcacccctta tctgggacgt ttttgcagta
     9181 agtacctatg caacagtaag tacggtgttt ttcattatag gtatgattcc ggacattgcg
     9241 gcaataagag acagggtagt agaaaaacca agaaaaatta tctatacatt actatctctt
     9301 ggttggaagg gttcaaactg ggaatggctg cactatacaa gggcttatct ttattttgct
     9361 gcctttgccg ctcctcttgt aatatcggtt catagtgttg tgtcatggga ctttgctatg
     9421 gcaaatactc ctgggtggca tacaactata ttcccgccat attttgtggc aggtgctatt
     9481 ttctcggggc ttgcaatggt tattaccctt tgtataccaa ttagaaagat attccactta
     9541 gaagattata tcacaatgga taatttcgat gccatggcaa aaatgattat tcttacatca
     9601 ggtattgtcg gatatgccta tggagcagag ttttttatgg cttggtacag tggcaaccct
     9661 catgaatggg gacagtttct ttatagggca accgg
//
LOCUS       DBNGSV010000150         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1342393, whole genome shotgun sequence.
ACCESSION   DBNGSV010000150 DBNGSV010000000
VERSION     DBNGSV010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1342393"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1142
                     /locus_tag="AC159U_05255"
     CDS             165..1142
                     /locus_tag="AC159U_05255"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR3336497.1"
                     /translation="MMTRTVTLSNLIFENGLVSGTMNENGGAIGATGFPFADLVIQDC
                     TFRNNRVQCEGTSCQARGGAISAAGQLTIVRSTFEGNTTHCSVNNQQCFTRAGTVEHD
                     SNSSLTISNSTFANNQTSCEGTGDSSCTLEGGALHISSSGSVMNNNTFADNTILCDAS
                     TCSLFGSTIYIDGTGPDTTNTIITNALGVVPPNCDGTLTDSGNNLQFPVNSCGGTIPT
                     EDADPEPLADNGGITETMAITEESPAFNEGNNGTCEDTDQRGVSRPQEGTCDIGAYEL
                     IPDDEPPAPPAPSEPVVIVPTMGQWGMIILAVFSILISITALRNRNENT"
     gene            complement(1222..1536)
                     /locus_tag="AC159U_05260"
     CDS             complement(1222..1536)
                     /locus_tag="AC159U_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019643.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HIR3336498.1"
                     /translation="MRFSDIGENDTAYSHRNAAIALNINASWDDPCEEKTHIEWTRNF
                     WKALQQFSTGGSYINFQSSDEGEERVKSTYGDDKLKRLTALKTQYDPYNLFRLNQNIK
                     PG"
     gene            complement(1526..>2560)
                     /locus_tag="AC159U_05265"
     CDS             complement(1526..>2560)
                     /locus_tag="AC159U_05265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3336499.1"
                     /translation="EVLLGDHPEYEEVRKIWNGMIDKKPAMIARCKGVSDVIDCINLA
                     RENNILISVRGGGHNVSGNAVCDEGLMIDLSLMNSVRVDPNNKTANVSPGCCLGEVDH
                     ETQKFGLIVPAGIVTTTGAAGLTLGGGFGWTSRKYGLTCDNVISYDVVTANGQFVKAS
                     KDENPELFWGLRGGGGNFGIVTNFEYRLHDFGPEVAAGLCFFPFEYAKDVLNFYQQYS
                     EEVPNEVSMICVLRCVPDTPIFPKSEIGKPMVALGFCYSGPAQEGLKYAKPIGEIAKP
                     YANGIKSMIFRDLQSMLDAGQPKGKKYYWKAENLKPLSYDCIDTLVEHVSNITSPTTL
                     VPLFQMKGAI"
BASE COUNT          748 a          576 c          475 g          761 t
ORIGIN      
        1 cttaaatatc ttattatctg ctctggtgtg taacgctttc tgggcatttc ccttgcctcc
       61 tattcatagg gtttattacc taaaattcta acttataatc tggtactatt atatggggca
      121 aggtcaacaa catgacccgt atattttcgg tttccggcac tttcatgatg actagaacag
      181 ttacactttc taatcttatt tttgaaaacg gccttgtgtc aggcactatg aatgagaatg
      241 gcggcgcaat cggggcaacc ggctttccat ttgcagacct cgttatacag gactgcacat
      301 tcagaaacaa tagagtacaa tgcgaaggca cttcatgtca ggcaagggga ggagcaatat
      361 cagcagcagg ccaactcaca atagtaagat ctacattcga gggaaacaca acacattgta
      421 gtgttaataa tcaacagtgt tttacaagag caggtacggt ggaacatgat agcaatagtt
      481 ctcttaccat ttcaaatagc acctttgcta acaaccagac tagttgcgag ggcacaggag
      541 acagttcatg cacacttgaa ggcggtgcgc tccatataag cagcagcggt agcgtaatga
      601 ataataacac ttttgctgac aatactattc tgtgtgacgc ttcaacatgt tcactattcg
      661 gcagcacaat ttatattgat ggtacaggtc cggatactac caatacaata attaccaatg
      721 cactcggagt tgttcctccc aattgcgacg gaacattaac cgattcagga aacaatctac
      781 agtttccagt taacagctgt ggagggacta tccctactga agatgctgat cctgaaccat
      841 tggctgacaa tggcggcata actgaaacta tggcaatcac agaagaaagc ccggcattta
      901 atgaagggaa caacggcaca tgcgaagata ccgaccaaag aggtgtttca agaccccagg
      961 aaggcacctg tgatatagga gcttatgaat taatacctga cgatgagcca cctgctccac
     1021 ctgcaccatc tgagcctgta gttatcgtcc caacaatggg acagtggggc atgattatac
     1081 ttgctgtatt ttctatatta attagcatca ccgcattgag aaatagaaat gaaaatacat
     1141 aacacctatt aacaactaat tttattaaaa taaaaaggga gctacggctc ccttttttgt
     1201 attactattt tattatatct tctaaccagg ctttatattc tgattaagtc taaataggtt
     1261 ataaggatcg tattgtgttt ttaaggctgt aagcctcttt agtttgtcgt ctccataggt
     1321 ggacttcacc ctttcctcgc cttcatcact actctgaaaa ttaatgtacg atccgccggt
     1381 tgaaaattgc tgaagagctt tccagaaatt tcttgtccat tctatatgtg ttttctcttc
     1441 acaaggatca tcccaggaag cattgatgtt aagcgcaata gctgcatttc tgtgactgta
     1501 cgccgtatca ttttcaccaa tatcactaaa tcgcaccttt catttgaaat agaggcacaa
     1561 gggttgttgg tgaagtaata ttcgaaacat gctctacaag cgtatctatg caatcatatg
     1621 aaagtggttt aaggttttca gctttccagt aatatttctt acccttgggc tggccggcat
     1681 caagcatgct ttgtaaatct ctaaatatca tagatttaat tccatttgca tatggttttg
     1741 caatctcacc aataggtttt gcatacttca agccttcttg agccgggcct gaataacaaa
     1801 atccaagcgc taccattggt ttacctattt cactcttagg aaatataggg gtatccggca
     1861 cacagcggag aacacatatc atacttacct catttggtac ttcttcagaa tattgctggt
     1921 agaaattaag cacatctttt gcatattcaa agggaaagaa acaaagacct gcagcaactt
     1981 ccgggccgaa atcatgtagt ctgtactcaa aattagttac tataccaaaa ttgccgccac
     2041 ctccccttag tccccagaag agttcaggat tttcatcttt actggcttta acaaattgtc
     2101 catttgcagt tactacatca taagaaataa cattatcgca tgtaagtcca tactttctcg
     2161 atgtccatcc aaacccacca ccaagcgtaa gccccgcggc tcctgtagtt gttacaattc
     2221 ctgctggtac aatcagtcca aatttctgag tttcatgatc cacctctcca aggcagcagc
     2281 cgggagaaac atttgcggtt ttattgtttg gatcgacccg tacagaattc atgagtgata
     2341 gatcaatcat aagcccctcg tcacaaactg cgttacctga aacgttatgc cctccccctc
     2401 tcaccgagat aagaatgtta ttttctcttg caagatttat gcaatctata acatctgata
     2461 cacccttgca ccttgctatc attgcaggct ttttatcaat cataccgttc catatttttc
     2521 ttacttcttc atattcagga tgatcgccta acagtacttc
//
LOCUS       DBNGSV010000151         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1897249, whole genome shotgun sequence.
ACCESSION   DBNGSV010000151 DBNGSV010000000
VERSION     DBNGSV010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1897249"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..838
                     /locus_tag="AC159U_05270"
     CDS             179..838
                     /locus_tag="AC159U_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336500.1"
                     /translation="MRRNILFLFSLFIFVVSVSGAGQVYAGGKHVMVSPTSATKAADV
                     RVALNSLLREHVFLATAATGAALGGRSDEFQSAATALDGNSVDIANAIGSIYGEDAGE
                     AFLPLWRKHIGFFVDYTTGVATKDEAKQQKAVSDLLGYTKDFAAFLNSASPALPVDTV
                     AELSKTHVITLKAVVDTQATGNSEEAYKAIRKASAHMQMIADPLAETIVQQFPNTYAV
                     K"
     gene            919..1314
                     /locus_tag="AC159U_05275"
     CDS             919..1314
                     /locus_tag="AC159U_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR3336501.1"
                     /translation="MKLLKMIMIFTIMNVFYISAATYFMSFADSIEAETVLEKSNGDQ
                     VEVDIKLFQYKPADIEVSTGTTVIWKNNDAIEHSITHGTPENSGDEFDSEFFTKEQSY
                     SMKFEKEGEYPYFCKRHPSMKGKVVVTSR"
     gene            complement(1385..1470)
                     /locus_tag="AC159U_05280"
     tRNA            complement(1385..1470)
                     /locus_tag="AC159U_05280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1435..1437),aa:Ser,seq:tga)
     gene            complement(1540..2763)
                     /locus_tag="AC159U_05285"
     CDS             complement(1540..2763)
                     /locus_tag="AC159U_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3336502.1"
                     /translation="MFIDFFYLLKNDGLPVTLREYLTLLEALDKEVISYSVEDFYYLS
                     RTTLIKHEQHLDRFDKLFGVFFQGIEFIPTEEFMKIPDKWMKRGFERILTEEDKALIK
                     SMGGLDKLMERLKELMKEQKKRHAGGNKWIGTGGTSPFGAYGFNPEGIRIGQDRSINR
                     RAVKVWDKREFKNLDDNVEIDTRNMKMALRKLRVLTREGMPEELDIDKTIKKTSENAG
                     LLELEMVASKKNNVKVLLFLDIGGSMDDHIEICAQLFSAAIYEFKHLQYFYFHNCIYD
                     HIWKNNIRRYEERTPTFEVFNTYNSDYKLIIVGDASMSPYELAMPGGAVEYMNEEPGY
                     TWLQRLKDQYPDFIWLNPIDPDDWKWTESIGMVREFFEGRMFPLTIAGLQDAVSLLKK
                     GKRSPAKKLGEVKYA"
     gene            complement(2767..3600)
                     /locus_tag="AC159U_05290"
     CDS             complement(2767..3600)
                     /locus_tag="AC159U_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3336503.1"
                     /translation="MKFKGTDTYIATEDLMVSVNAAISLEKPLIIKGEPGTGKTLLAF
                     EIAKALSKELIAWHIKSTTTAQQGLYEYDAVTRLRDSQLGMEEVADIANYIKPGKLWE
                     SFDSREQVVLLIDEIDKADIEFPNDLLLELDKMEFYCYELQKTIKAKKRPIVVITSNN
                     EKELPDAFLRRCFFHYIAFPERDTMKEIVDVHYPDLEDKLLQSAMRVFYEIRDLNGIK
                     KRPSTSELIDWIKLLVLGKITSEELDDIDVDNELPPYVGSLLKNEQDHGLIRLVKRRF
                     R"
     gene            complement(3678..4352)
                     /locus_tag="AC159U_05295"
     CDS             complement(3678..4352)
                     /locus_tag="AC159U_05295"
                     /inference="COORDINATES: protein motif:HMM:NF040120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HIR3336504.1"
                     /translation="MLERIKTTSGLVLIVSNIIPIYGVFYQSWDAFWIVLLYWSENLI
                     IGFYNVLRMIFVKASTPAENVSKLFMIPFFIVHYGGFCAGHGFFIMMMFKRDPGNLFE
                     DISAPGFFVFIEMLISVVRKILEILPDNMLLAFFTLFLSHGFSFVYNFIIKGEYMNVK
                     AKDLMSQPYIRIGVLHIAIIAGAFPVMYFGSPVYLLVVLILIKIIIDLYLHNKEHKKF
                     QKEISV"
BASE COUNT         1318 a          873 c          818 g         1429 t
ORIGIN      
        1 agcaagtttg ctggatttaa tgaaatagac ttttagtaac ttagtagtag ttttagtatg
       61 attttatatg gcaggcctgt atctgaactc attatgacaa taagaagatt cttatattca
      121 ttcatccaaa cctctattta actccgtata tatctaaaaa tttataagga ggtgcaagat
      181 gagaagaaac atcttgttct tattttcatt atttatattt gtagtatcag tatcgggtgc
      241 aggccaggtt tatgcaggtg gtaaacatgt aatggtatcc ccaacatctg ctacaaaggc
      301 tgccgatgtg agggttgcat taaattctct tttaagagaa cacgtgttct tggcaacagc
      361 ggctacaggt gcagcgcttg gaggaaggtc cgatgaattt cagtctgctg ccacagctct
      421 tgatggaaat tctgttgata tagcaaatgc tatcggatcc atttatggtg aagatgctgg
      481 tgaggcattt cttccacttt ggagaaagca tataggattt tttgttgatt acacaaccgg
      541 ggttgctaca aaggatgaag ccaagcagca aaaggccgta agtgatcttc ttggatatac
      601 aaaagacttt gcagcttttc taaactctgc aagtcctgct ctgccggtag atacagttgc
      661 tgagttatca aagactcatg ttattacgct aaaggcagta gtggatacgc aggctacagg
      721 aaacagtgaa gaggcttata aggcaataag aaaagcatca gcacatatgc agatgatagc
      781 cgacccactt gctgaaacca tagtacagca gtttcctaat acatacgctg taaaataata
      841 tttattcata tttaaaatat attctggggc aggccgcaac accggtctgc tccagcactt
      901 acatggagaa ttatgattat gaaactatta aaaatgataa tgatctttac tattatgaat
      961 gtgttttata tatcagcagc tacatatttt atgagttttg ccgatagtat cgaggctgaa
     1021 accgttttgg aaaaaagtaa tggggatcag gtcgaagtag atatcaagct atttcagtac
     1081 aaacccgcag acatagaagt tagcaccggt actacagtaa tctggaaaaa caatgatgcc
     1141 attgaacaca gcataacaca tggaaccccg gaaaattcag gagatgaatt tgattcagaa
     1201 tttttcacaa aagaacaaag ctattcgatg aagtttgaaa aagaaggtga atatccttac
     1261 ttttgcaaaa gacatccatc tatgaagggt aaggttgtag ttacttccag ataagccgta
     1321 tataaaatct aaacagtctg gcaaagagaa aacataagaa tatttacgaa aatagagtaa
     1381 atattggcgg agggcgaggg attcgaaccc ccaagtccgc aaggacgccg gttttcaaga
     1441 ccggtgcatt agccgttctg ccagccctcc gatttgagtg ttttcattct acctaaagag
     1501 tttgttactt caaattaaat tgatagagaa tttggatact caggcatatt taacttcacc
     1561 gagttttttt gcaggacttc ttttgccctt cttaaggaga ctaactgcat cctgaaggcc
     1621 tgcaattgta agcggaaaca ttctgccttc gaaaaattcc cttaccattc caattgattc
     1681 tgtccatttc cagtcatccg gatcgatggg gtttagccat ataaaatcgg gatattggtc
     1741 ttttagcctc tgaagccatg tatatccagg ctcttcgttc atatattcca cagctccccc
     1801 tggcattgca agctcgtagg gagacataga tgcgtctcct acaataatta atttataatc
     1861 gctattataa gtgttaaaca cctcaaatgt aggagttctt tcttcgtatc gcctgatatt
     1921 atttttccat atatgatcat aaatgcagtt gtgaaaataa aaatactgca ggtgcttgaa
     1981 ctcataaatt gcagcagaaa atagctgtgc gcatatctct atatggtcgt ccatagagcc
     2041 gccaatatca aggaataaca gcacttttac gttgttcttt tttgatgcca ccatctcaag
     2101 ttctagtagc cccgcatttt cagaggtttt ttttatggtt ttatctatat ccagttcttc
     2161 tggcattcct tctcttgtaa gaacccttag ttttcttaga gccatcttca tatttcttgt
     2221 atctatctct acattgtcat caagattttt aaattctctt ttatcccata cctttacagc
     2281 cctgcggttt atgcttctgt cctgaccaat tcttatccct tcgggattaa agccataagc
     2341 tccaaaaggg gaggtgcccc cggttcctat ccatttattt ccgcctgcat gtcttttctt
     2401 ctgttccttc ataagctctt ttagtctttc cataagctta tccagtccac ccatggattt
     2461 tataagcgcc ttgtcttctt cggttaaaat tctttcaaac cctcttttca tccatttatc
     2521 cgggattttc ataaactctt cagtaggtat gaattcaata ccctgaaaaa atactccaaa
     2581 tagtttatca aacctatcaa ggtgctgctc atgtttgata agggtagttc tgcttagata
     2641 gtagaaatct tctacactgt atgagattac ttctttgtca agcgcttcca agagggtgag
     2701 atattccctg agtgttaccg gaaggccatc atttttcagt agatagaaaa aatctataaa
     2761 catagcttac ctgaatcttc tctttacaag tctgataagt ccatgatcct gctcgttctt
     2821 tagcagtgac cccacataag gcggcagttc attgtcgaca tctatgtcat cgagctcctc
     2881 ggatgtgatt tttccaagta ccagaagctt tatccagtct ataagctcgc ttgtagaggg
     2941 tcttttcttt attccgttta aatcccttat ttcatagaaa acccgcattg cgctttgaag
     3001 cagcttgtct tccaaatcgg ggtagtgtac gtcgacaatc tctttcattg tgtctctttc
     3061 cggaaatgcg atgtaatgaa agaagcatct tctaagaaaa gcgtctggga gctccttttc
     3121 attattggat gttattacta caatcggacg cttctttgcc ttaatagtct tttgaagctc
     3181 atagcagtaa aactccatct tatctagttc taacaataaa tcatttggaa actcaatatc
     3241 ggccttgtca atctcgtcta tcaacagcac aacctgttcc ctggaatcaa aagattccca
     3301 gagctttccg ggtttaatat agttagcaat atcggctacc tcctccattc caagctgaga
     3361 gtctctgagg cgggtgacgg catcatattc atagagtccc tgctgtgcag tcgtagtgga
     3421 ttttatatgc catgcaatta gctccttact tagagccttt gcgatttcaa aagctaatag
     3481 tgtttttccc gtaccgggct caccttttat tatgagaggt ttttccagtg aaattgcagc
     3541 gtttacagag accataaggt cttctgtagc aatatatgtg tctgttcctt taaatttaat
     3601 gttctttgcc atgtttttta atgtattcct ttataagaaa aattcaaatg tatagaaaat
     3661 tatacatagt gtttttacta aacagatatc tctttttgaa actttttatg ttctttattg
     3721 tgtaaataca gatcgataat aatttttatt agaatcaata ctaccaataa atagaccggt
     3781 gaaccaaagt acatcacagg aaaagcacct gcaataatag ctatatgaag tacgccaatt
     3841 cttatgtacg gctgggacat gaggtctttt gcttttacat tcatatattc acctttgatg
     3901 atgaagttat atacaaaaga aaacccgtgg cttaagaaaa gagtaaaaaa tgctaataac
     3961 atattatcgg gcaggatttc aaggatcttc cttactacac ttattaacat ttcaataaag
     4021 acaaaaaatc ccggggctga tatatcttca aaaagatttc ccggatctct tttaaacatc
     4081 atcattataa agaatccgtg tccggcgcaa aaccctccat agtggactat gaaaaagggg
     4141 atcataaata acttggaaac attttctgcc ggagtagatg cttttacgaa tatcattctc
     4201 aatacattgt aaaaaccgat tatcaggttt tcagaccagt agagaagcac tatccagaat
     4261 gcatcccagc tttggtagaa tactccataa atcgggatga tatttgatac gataagaact
     4321 aaaccagagg ttgttttaat tctttcaagc ataaaaatga tttaacttat tttatccaga
     4381 ccatccatat atggtcttag agcttctggg agggtgacag tgccgtcttt attctgaa
//
LOCUS       DBNGSV010000152         1564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_928498, whole genome shotgun sequence.
ACCESSION   DBNGSV010000152 DBNGSV010000000
VERSION     DBNGSV010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_928498"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..376
                     /locus_tag="AC159U_05300"
     CDS             149..376
                     /locus_tag="AC159U_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336505.1"
                     /translation="MGMKQLRKMTKCDMLLNLFDEIGKSLKVNIVCDITGIKNYNSLK
                     ALFSYIRKAPHIPDDHRIDVRIQDDICIRVN"
     gene            410..1309
                     /locus_tag="AC159U_05305"
     CDS             410..1309
                     /locus_tag="AC159U_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336506.1"
                     /translation="MTTREYIEKFKRSTGRLPSQVEMSKQLKISSAVALQELINYTRY
                     VAPEKKTRMRESKAKKDRTVILLKVFLVILSAMAFTLSVYFTGLWFSGRFSMFISGLI
                     SLSMVLFMVISPQTLRFVSNPFVKGVVILSFIVALVFSMSSTVAGQYQKTSEKIESEP
                     DKAYIFDQLLKGEVEIEQLINEAKSDKQIHQESIILLSGTEESRKDNWQSIATERKYI
                     ESFDQRIDSLRIELNNIRANKVDNGIIEERRDFYMFISGLTGMEKSFTEFILSSLPAI
                     FIDIISALCLNLALFIKKKEIDK"
BASE COUNT          570 a          216 c          314 g          464 t
ORIGIN      
        1 aaagaaaagt atgtaaaaga attagttcat ataatggaga actgtatcat gttaaaggtt
       61 cctttagtag tagatataga aagaggaaat aattggggaa gcgtgggtaa aataaaactt
      121 gactaaagta aaaggatgct ttacaattat gggtatgaaa caattacgga aaatgacaaa
      181 atgcgacatg ctcctgaatc tctttgatga gataggaaaa tcattaaaag tgaatattgt
      241 gtgtgacatt actggaataa agaactataa ttcattgaag gctcttttct cttatataag
      301 aaaagcacca cacattccag atgatcacag gatcgatgtt agaatccagg atgatatctg
      361 cataagggtc aattgaccct tattttttaa ctatgaggta aaaggaatta tgactactcg
      421 tgaatatata gaaaagttta agaggagtac tgggagatta ccttcacagg tagaaatgtc
      481 caagcagtta aagatatcct ctgcggtggc tcttcaggag cttattaact atacaagata
      541 tgtggctcct gaaaagaaaa caagaatgag agaaagtaaa gccaagaaag acaggacagt
      601 gattttatta aaagtctttc ttgttatact ttcagcaatg gcatttacgc tttcagtgta
      661 tttcactgga ctgtggttct cagggagatt ttccatgttt atatccgggc tgatctccct
      721 gtctatggtc ctgtttatgg ttatatcacc gcaaaccttg aggtttgtca gcaatccatt
      781 tgtaaaagga gtagtaatat tatcgtttat tgtagcattg gttttcagta tgtcctccac
      841 ggttgcaggg caataccaaa agacaagtga gaagattgaa tctgaacctg ataaggctta
      901 tatctttgat caactgttaa agggtgaagt agagatagag cagttgatca atgaagctaa
      961 gtcagacaag cagatccacc aggagtcgat tatccttcta tctggtacag aagaaagcag
     1021 gaaggataat tggcaatcta ttgcaacaga gagaaagtac attgaatcat ttgaccaacg
     1081 tattgattca ttgagaatag aattaaataa tattcgtgca aataaagtgg ataatggtat
     1141 aattgaagag agaagagatt tttatatgtt tatatcggga ttaacaggaa tggaaaaatc
     1201 atttacagaa tttattctct catcactccc ggcaattttt atagatatta taagtgcact
     1261 atgcctgaat ctcgctttat ttatcaagaa aaaggagata gacaaatgaa taaagagtta
     1321 aaaaagaaat gtactacatg tggaaaagag tgcaatgtta aagaaatgaa aacaaccatg
     1381 tcaaatcaaa tgcatagata tgtatgctcc agtaaatgca taattgatta ttacaaataa
     1441 cacatatacg gaggatatat gacaccaata attattatta tatcattagc tattatttgt
     1501 ttggtagttc tcgcattcga taatggtgat gattaaccac tacctataga tatgataagg
     1561 aaac
//
LOCUS       DBNGSV010000153        11095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_512599, whole genome shotgun sequence.
ACCESSION   DBNGSV010000153 DBNGSV010000000
VERSION     DBNGSV010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_512599"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /locus_tag="AC159U_05310"
     CDS             <1..85
                     /locus_tag="AC159U_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3336507.1"
                     /translation="KKTAEILGINRNTLAKKMAQLEIDNQE"
     gene            complement(110..778)
                     /gene="rnc"
                     /locus_tag="AC159U_05315"
     CDS             complement(110..778)
                     /gene="rnc"
                     /locus_tag="AC159U_05315"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3336508.1"
                     /translation="MKDQILGYTFKDKELLRTALTHSSYANECSVQSNERLEFLGDAV
                     IGCVTAKVLYNHFPDYPEGNLSKIKSAIVSRENLGVFARNINLQDELLLGKGEESSGG
                     RDKMSNLSGAFEAVIGAIYLDSGYTKVYSVISKLIKDCLRQNSFDDDYKTNFQEISQK
                     LFKSIPRYKVVQEEGPSHNKSFYVEVEINKKVFGNGCGKSKKSAEQEAARQGLDKIKD
                     IARL"
     gene            complement(873..1865)
                     /locus_tag="AC159U_05320"
     CDS             complement(873..1865)
                     /locus_tag="AC159U_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3336509.1"
                     /translation="MPVQSQYGRSRTFLNTQIGDSNYFVNMSWVQSIERTDNLKRQPG
                     SGNIIGVLSFERINIDVFSFSSLLNLEHSEEDFVSERVVVIKKQDIRMGLLVNSVSGI
                     VEIQQDLVFGIPRIADSNISDYFENIAISEDELYLSISPDFIAGRLSDNINESEANDK
                     IIAQTNKIDQNGTNGRTTGSNQILLFQMLDEDGKLSEYVFGMSISQILQILEPQKVLD
                     VPGSPDFVYGLINWRNRPVPVIDLYKRIGLKNSKITDQTRFVVARTLTGGHIVCFPVY
                     FDVNVKKLPLPHQKLPLGSDIDMSLTKGTFKAEDYSLVIPDMDKIVFSKISNIN"
     gene            complement(1807..2979)
                     /gene="cheB"
                     /locus_tag="AC159U_05325"
     CDS             complement(1807..2979)
                     /gene="cheB"
                     /locus_tag="AC159U_05325"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HIR3336510.1"
                     /translation="MSKLTKVVLADDSPFVCSMLKNYLQSTTEFEILGVAYDGLEALD
                     LVKQTRPDVLTLDLEMPKMNGLEVIEHVMYECPTPIVVISGISRNASDITLKALEMGA
                     VDFILKYTPGIEINPEYLQADIISKVKGASNIRVIRSLKSHEHYKANKDTKLYPFFDY
                     APIKKAPAGISYHSTQDNIIEAGVLVVGASTGGPVALKELINHLPDNYPQAIVVVQHM
                     NSSFTRVLASQLNKNTNFRVKEAEDGDMLMKDLILIAPGDYHLMIGSDLRVKLTKGPK
                     VKGHRPSIDVTMQSVSQVYGSKTKGVVLTGMGDDGAMGLLAIKSKWGSTYVQEPSDCV
                     VDSMPKSAIKKGVVDYIEPCWKIASLLGSNISKGSMKSASSKSVRKKQNILKYPNW"
     gene            complement(2976..7391)
                     /locus_tag="AC159U_05330"
     CDS             complement(2976..7391)
                     /locus_tag="AC159U_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3336511.1"
                     /translation="MSKSIDQQLIAGFVGEAKSYIPNIESAIESLRGGVDRISKIEEM
                     HRYIQNIKGASSMIGVTELSDISNYMEKLLEDLAANKVEYSDEVLLFLRNTTTHIGKY
                     LDSLVDGEFDYKSVVNEVKAAYIKLGGKEAQTEKVEFDKEQTIAPEDSTYTESTSEST
                     ADTQYPENEPLIAGPLSIYKELLNKFGELQDAPESYEKINELKKLVSELKDNCNVSEF
                     LPADKVSAAINFVSDISSNLLKIVNDIDAHKVTGNYETLLGIAETFIYQIERVLYNYR
                     SEIDFESEVGQLFSNYEEFHDKVLAGEQNDLQNVDADSADNKDGAMSPELIDGFISET
                     KKDINAVINEIENYAVSTDKKSIINSVKSHIHKIHGASGMLGFDNPQNANNERNHHGI
                     FLISKELSEFLNLVSDSDTELDAQSAGVVVQGTLLLKKLCEDSQVQNVEEEISNISSV
                     VSQLSSNILQSPQVNVEQDAVSLDPPAVQERKPQKQIIPDELFEVFTIEANEHLENMN
                     KCLSVYKSTNDLQDLQEVRRGAHTIKGAAGMVGLQSVSDLGHEMESVLDNIFERNIQP
                     SKKVLDLFSITTTFLEKLISNGDNVGTDSELNDILSHYSNIDLFDELSYEQATAAIDT
                     EEISIPDDIPQVEIEHERQDAEKVVSEVSTEQPQFMFYSESTEDIPPDLLEVFLIEAN
                     EHLQNMGRYLNMLVEDNKDRDSLQEIRRSVHTIKGAAGMVGFSSVSTLAHRMEDLLDN
                     LYENSVVLNDEIINLLYRTSDLLDDIVNSKVTDLQGNKLLTSLYDEYKAFVKEDDSEK
                     IPAEQVFADTPDQFIQDDVFLGLNTEEIFNVEEVQQEPHQRQEQAIRKPGDFVRIPME
                     RLDELVNLVSELVVNRSTFEQHFGVFNQELDEMAPSLDRLRRVTSNLETQYEVLTLGG
                     GKLQMAAGDESNVSSLPVHTSQTHGFDELEFDRYTEFHQYTRELSESTSDIRAIGGQM
                     AGLTGDFESYLNRQERITSEVQDKLMRLRMVPLATVATRLHRAVRVTASQQNKKVQLN
                     IVGEDVELDKTVLEEMSDPLLHIIRNSVDHGIELAELRKALGKDEVGTITLKAYYEGT
                     QVVIQIGDDGAGLVPEVLRTAAVRGGFISESDSHRISDEELFKLIFLPGFTSTTEISE
                     ISGRGVGMDIVGDTVTKLKGTVSIDSEASKGVTFTIRLPMTLAITRVLLVYANNEQFA
                     VPLASVSQIMRVEKEYIENLGDERVLKIENNIYPIIRLGEALNLKTSSSESITSLPVL
                     IMNLGEQHIALVVDELLESREVVVKTLGNHLKNVHGITGATLMGDGSVVLIVNPSDLI
                     DKPSSATVDINVPVSTEFRRDILDVFIVDDSLSVRRIMTNLIKNSGWNPIVAKDGVEA
                     LEILQRSVKLPDVILLDIEMPRIDGYELTSTLRAQQQFKDIPIVMITSRAGKKHRDKA
                     FELGANDYMVKPYQEEDLIGLIKKITGSTSNTSLQ"
     gene            complement(7407..8309)
                     /locus_tag="AC159U_05335"
     CDS             complement(7407..8309)
                     /locus_tag="AC159U_05335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3336512.1"
                     /translation="MNINTSTAYEDSDSLSLQKLEQYELIEQEFSRWQQCIESKCGLY
                     FDKSRISFLCTALLERINDLSLTGYDEYLRYLLYNNDAEREWKELYCRLVNCETSFFR
                     HQPSFELLKEHIFPQVLESRKNDIFKLLTVWSVGCSSGQEAYSIALNALQSYDTDEYY
                     TKIIGSDINLHLLKKARKGIYTKAEVKSLHPEYIERYLFPYNGSGTTYYKFRDEVKDL
                     VSFNFINLVESNTYKVFPVDIIFCQNVLFYFSKNRRLEIVYRLCEYLGKGGSLILGPS
                     DILPARINGMETEYINSNLVYRKL"
     gene            complement(8399..10717)
                     /locus_tag="AC159U_05340"
     CDS             complement(8399..10717)
                     /locus_tag="AC159U_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3336513.1"
                     /translation="MRLWQKLVLLVLVPIIISVPVILYFLKVELDNKENLARRQFAGI
                     EYIQPLRDYGDIIEDFRTVTIDVLENNRQLDDQTQAELDRLKKSMEDKIAQVDALNNK
                     YGDIFGLTGVWGSLKTGCEQLIATALELSAEDSYLVQKEHETEISDFIIQIVNSSQLK
                     NEIADVFLAELPNLATDIKDIERLSELAIRKSSDRNVYQEGVDGEIIVQLKQDGVSRD
                     EISDQLLLVENNLEIIKRGSELYLATNIDLRSQLRPLILKLERDISVFVEDINNDIVR
                     PATVTTTKQSINEKVETVSASIFAPYDSALQAIENLLSARIQQVGNQKIIILLVIGAA
                     ILLSLLIAVLVLRGVLGQIDFIRSAMGAYTEGDVSARSIMSSSDELGEMAGSVNQFMD
                     ENEALTNKVKEDAARVEDEYNRTQESIIKLLDEVSGIAEGDLTQEAEVTAEVTGAIAD
                     SFNNAIVELRRIITEVQSASTRVNEASSDIRLSMDRLSQGSEEQSGQIIDTTAAIDEM
                     AISIRQVSENASMSATVAQQALSNAKQGAEVVENTITGMNNIRDQVQETSKRIKRLGE
                     SSQEIGEITQLIGEIADRTSILALNASIQAAMAGEAGRGFAVVAEEVERLADRATDST
                     KRIETLIKTIQAESNDAISAMEDMTNEVVAGSERAADAGKALGEIESVSVKLADLIQS
                     ISMAAQQQARGSETIANAMSDISMVTQQSTLSTREAFTTSAGLTELSEELGRTISAFK
                     LPETNGASPEFTTSDVDIPSAESVTPAEGATQ"
     gene            complement(10925..11095)
                     /locus_tag="AC159U_05345"
     CDS             complement(10925..11095)
                     /locus_tag="AC159U_05345"
                     /inference="COORDINATES: protein motif:HMM:NF025063.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3336514.1"
                     /translation="METWRRDYNEYRPHSALDNMTPEEYVKEHKKPEISNYPGTAFVL
                     GEGQEHLIKFKN"
BASE COUNT         3167 a         2451 c         1746 g         3731 t
ORIGIN      
        1 gaagaagact gcagagatat tagggattaa tagaaataca cttgcaaaga aaatggctca
       61 attagagatt gataaccaag agtaataaaa tattttaatt gatactctat tataacctag
      121 ctatatcttt aattttatca agtccttgtc tggcagcttc ctgctctgct gattttttgc
      181 tctttccaca cccgtttcca aatactttct tgtttatttc gacctctacg tagaaacttt
      241 tattatgtga aggcccctct tcctgaacta ctttatatct gggtatgctt ttgaaaagtt
      301 tttgagatat ttcctgaaag ttggttttgt agtcatcatc aaagctattt tgcctcagac
      361 agtcttttat tagctttgat attaccgaat aaacctttgt ataaccgcta tcgagataaa
      421 tcgccccaat tactgcctca aacgccccgg agaggttgga cattttatct ctgccaccgc
      481 tagactcttc cccctttccc aaaagtaatt catcctgaag gttgatattc cttgcaaaca
      541 ccccgagatt ttccctgcta acgattgcac ttttaatttt tgataaattt ccttcaggat
      601 aatccgggaa atgattataa agaaccttgg cagttacaca gccaattaca gcatcaccca
      661 aaaactcaag cctctcattg ctttgcaccg agcactcatt ggcatatgaa ctgtgagtaa
      721 gagctgttct aagtaattcc ttatccttaa atgtatatcc gagtatctga tctttcattt
      781 gcgaaatatt actaaaagaa ggtctttgtt aaaagatggc aaatattagt ttactaatat
      841 tttataaaaa aacacacaaa tttcaaataa ttttagttta tattactaat tttactaaat
      901 actattttat ccatatcagg aatgacaaga ctataatcct cagctttaaa agttcctttt
      961 gtaagcgaca tatcaatatc agatcccaat ggtagttttt ggtgtggaag cggcagtttt
     1021 ttaacattta catcaaaata aacgggaaaa caaacaatat gacctcccgt tagtgtcctg
     1081 gcaactacaa accttgtctg atctgtaatt tttgaattct taagtcctat tcttttgtac
     1141 aggtcaataa ccggcactgg cctgtttcgc cagtttatca agccatatac aaaatccgga
     1201 cttcccggaa catctagtac tttttgaggc tccaatatct gaagtatctg tgaaatactc
     1261 attccaaata catattcact caacttacca tcttcgtcca acatttgaaa taaaagtatc
     1321 tgatttgatc cagttgttct accatttgtg ccgttttgat caattttgtt cgtttgggca
     1381 ataattttat cattagcttc actttcatta atattatcac tcaatctacc tgcaatgaaa
     1441 tcaggagaaa tagatagata taattcgtct tcgctgattg ctatattttc aaaatagtca
     1501 gaaatgttac tatccgcaat tctgggtata ccaaaaacca ggtcttgttg aatctcgact
     1561 ataccagaaa ctgagttaac caataacccc atacgtatat cttgtttttt tatcactact
     1621 accctttcac taacaaaatc ctcttcgcta tgttccagat ttaacaaact ggaaaatgaa
     1681 aaaacatcga tgtttattct ttcaaaactt aacactccaa taatatttcc agagcctggt
     1741 tgtcttttta gattgtcagt tctttcaata ctctgaaccc aggacatgtt gacaaaatag
     1801 ttgctgtcac caatttgggt atttaagaat gttctgcttc ttccgtactg actttgaact
     1861 ggcactcttc atactccctt tacttatatt actgccaagc agtgacgcta ttttccagca
     1921 gggttctata taatccacta cacctttttt tattgcactc ttgggcatgc tatctacaac
     1981 acaatctgat ggctcctgca cgtaggtact gccccatttg gattttattg ccaacagtcc
     2041 cattgcacca tcatcaccca ttccagtgag aacaactcct tttgttttag aaccatagac
     2101 ctgagaaact gactgcatgg ttacgtctat agaaggtctg tgccccttaa ctttaggacc
     2161 ctttgtaagc tttaccctta aatcagagcc tatcataaga tgataatcac ccggagctat
     2221 gagtattaaa tctttcataa gcatatcccc atcttcggct tccttcactc tgaaatttgt
     2281 gttcttgttt agctgtgatg ctaaaacacg agtaaaactc gaattcatat gctgtacaac
     2341 tacaattgcc tgaggataat tatcaggcag gtgatttatt agttccttta gagcaaccgg
     2401 ccctccggtc gatgcaccaa ctacaagcac acctgcttca ataatattgt cctgagtaga
     2461 gtgatagcta ataccggcag gagctttctt tataggtgca tagtcaaaaa acggatagag
     2521 cttggtatct ttattagctt tataatgttc atgggattta agtgatctta ttacccttat
     2581 attagatgcc ccttttacct ttgatataat atcggcctgt aaatattcag gattaatttc
     2641 aattcctgga gtatatttta agataaaatc aactgcaccc atctctaaag cttttaatgt
     2701 aatatcggat gcatttctac taattccact tattacaact attggggtcg ggcattcata
     2761 cataacatgc tcgataacct caagcccatt cattttaggc atttcaagat caagcgtaag
     2821 cacatcaggc cttgtttgtt ttaccaggtc cagagcttca agcccatcgt aagcaacccc
     2881 caagatttca aattcagttg ttgattgcag atagtttttc aacatactgc aaacaaaagg
     2941 agaatcatct gccagaacaa cttttgttaa cttactcatt gcaaagaagt attagatgtg
     3001 cttcccgtta tttttttgat tagtccaatg aggtcttctt cctgatatgg ttttaccata
     3061 tagtcattag ccccaagttc aaaagcctta tccctgtgtt ttttaccggc tctggatgta
     3121 atcatgacaa ttggtatatc tttgaactgc tgctgagctc tgagtgttga tgtaagttca
     3181 taaccatcga ttcttggcat ctcaatatct agtaaaatca cgtctggaag cttcaccgat
     3241 ctttgcagga tttcaagcgc ttctacacca tctttagcaa ctatgggatt ccaaccacta
     3301 ttctttataa ggttagtcat aattcgtctt acactaagtg aatcatctac aatgaataca
     3361 tcgagtatat ctcttctaaa ttcagtggat acaggaacat ttatatccac cgtagcacta
     3421 gatggtttat ctattaaatc tgatggattt actatcaaga caacactacc atcacccatg
     3481 agtgttgctc ccgtgatacc atgaacattc ttgaggtggt ttccaagagt ttttaccact
     3541 acctctctgg attccaacag ctcatccact acaagagcta tgtgttgttc accaagattc
     3601 attataagca ccggcaaact agttatggat tcacttgaag aagttttaag atttagagct
     3661 tcaccaagcc ttatgatcgg ataaatatta ttttctattt ttaagactct ttcatcccct
     3721 agattttcta tatactcttt ttcaactctc ataatctgtg aaacagaggc aagtggtact
     3781 gcaaactgct cattgtttgc ataaaccaaa aggactctgg taatagccag ggtcattgga
     3841 agtctaattg taaaagttac gcctttcgat gcttccgaat caatcgatac cgtacccttc
     3901 aattttgtta cagtatcacc tacgatatcc atccctactc cacgacccga tatctcactt
     3961 atttcagtag ttgaagtaaa tcctggaagg aatattaatt taaacagctc ctcatctgag
     4021 attctatgtg aatctgattc gcttataaac cctcccctta cagctgcagt tcgcagtact
     4081 tcaggtacaa gcccagctcc atcgtcccca atctggatta ccacctgagt accttcataa
     4141 tatgccttta aagtaattgt acctacttca tcttttccca gagcctttct aagctccgca
     4201 agttcaatac catggtcaac agaatttctt ataatatgca aaagcggatc agacatttct
     4261 tccaacactg ttttatccag ctctacatcc tctcccacaa tgtttaactg cactttttta
     4321 ttttgctgag acgctgtaac ccttactgcc ctatggagcc tggttgcaac agtggcaagt
     4381 ggtaccattc gaagcctcat gagcttatcc tgaacctcag aagttatcct ctcctgtctg
     4441 tttagataac tttcaaagtc tcctgtaagg ccagccatct gcccaccaat tgcccttata
     4501 tcacttgtag actccgacaa ctcgcgggtg tattgatgaa attctgtgta acggtcaaat
     4561 tcaagttcat caaatccatg ggtttgtgat gtgtgtacag gcagcgaaga gacattgctc
     4621 tcatcgcctg ccgccatctg caattttccg ccacccagcg ttagaacctc atactgggtt
     4681 tcaaggtttg atgtaaccct gcgcaatcta tccagactgg gggccatctc atctagttcc
     4741 tggttaaata ctccgaaatg ctgttcaaat gtagacctgt ttacaacaag ctcactcaca
     4801 agatttacaa gctcgtcgag tctttccatt gggattctta caaaatcccc aggcttcctt
     4861 attgcctgtt cctgtcgctg atgaggttcc tgctgaactt cttcaacatt gaatatctct
     4921 tcagtattaa gacctaagaa tacgtcatcc tggataaatt gatcaggagt atcagcaaat
     4981 acctgttcag ccggtatttt ctcagaatca tcttctttta caaatgcttt atattcatcg
     5041 tagaggctag taaggagttt attaccctgt agatctgtaa ctttactgtt tacaatatca
     5101 tcaagcagat cagatgttct ataaagcaaa tttattattt catcgttaag aacaacagaa
     5161 ttttcatata aattatcaag gaggtcttcc attctgtgtg caagcgtgga tacgctgcta
     5221 aatcccacca tacccgcagc accttttatt gtatgtacac ttcgtcttat ttcctgaagc
     5281 gaatctctat ccttattatc ctcaaccaac atgtttaaat atcttcccat gttttgcaaa
     5341 tgttcatttg cttcaattaa gaatacttct aacagatcag gaggaatatc ctcggttgat
     5401 tccgagtaaa acataaattg aggttgctcg gtactcactt ctgatactac tttctcggca
     5461 tcctgccttt catgctctat ttcaacttga ggtatatcat caggaattga aatttcttca
     5521 gtatcaatag cggcggttgc ctgttcataa gacagctcat caaatagatc tatgttgctg
     5581 taatgactta aaatatcatt taactcacta tctgtcccga cattgtctcc gtttgaaatg
     5641 agtttttcca agaaagtggt agttatagaa aataaatcca atactttttt tgacggctgt
     5701 atattacgtt caaaaatgtt gtcaagtaca gattccatct catgtccaag gtccgataca
     5761 gactgtagcc ccaccattcc agcagcacct tttattgtat gtgcccctct tctcacttcc
     5821 tgtaagtcct gcagatcatt agtggactta taaactgaaa gacatttatt catattttcc
     5881 agatgctcat ttgcttcaat ggtaaatact tcaaacagct catcgggtat aatctgcttt
     5941 tgtggttttc tttcctggac tgccggtggg tccaggctta ctgcatcctg ctcaacgtta
     6001 acttgtgggc tttgcagtat attagaagat aactgcgaca ctacagagct tatattgcta
     6061 atctcttctt caacattttg cacttgtgaa tcttcacata gtttttttaa taataatgtc
     6121 ccctggacta caactcctgc cgactgggca tctagctcag tatcactgtc agaaacaaga
     6181 tttagaaatt cactcaattc tttactaatc aaaaatatac catggtgatt tctttcatta
     6241 tttgcatttt gaggattatc aaaaccgagc atgccagaag caccatgaat tttatgaata
     6301 tggcttttta cgctgtttat tatggatttt ttatcagtag acacagcata gttttcaatt
     6361 tcatttatca cagcattaat atctttttta gtttcagaga taaatccatc aataagttca
     6421 gggctcattg ctccgtcttt attatctgca ctatcagcat ccacattttg taaatcgttt
     6481 tgttcaccag ctaatacctt gtcatggaat tcttcgtagt tgctaaacag ctggccaact
     6541 tcagactcaa aatctatttc acttctgtaa ttgtataaaa ctctttctat ctgatatatg
     6601 aaagtctcgg ctattccaag aagtgtttca taatttccgg taaccttatg agcatcaatg
     6661 tcatttacaa tttttagcag atttgatgat atgtctgaga caaaattgat agcagccgat
     6721 actttatcag ctggcagaaa ctctgaaaca ttacaattat ctttaagttc agatactaat
     6781 ttctttaatt cattaatctt ttcataactt tccggagcat cttgtaactc accgaattta
     6841 ttaagtaatt ctttataaat tgatagaggc ccggcaataa ggggttcatt ttcaggatac
     6901 tgtgtatcag cagtagactc agaagtactt tcggtgtagg tggagtcttc aggggcaata
     6961 gtttgctcct tatcaaattc tactttttca gtctgtgctt ccttaccacc aagttttata
     7021 tatgcagcct taacttcatt aactacagat ttatagtcaa actcaccatc tacaagacta
     7081 tccagatatt ttccaatatg cgtggtagta tttcttaaaa agagcaaaac ttcgtcacta
     7141 tactccacct tattggcagc caaatcctcg agcagcttct ccatataatt tgagatgtca
     7201 cttaactcag taacacctat catggaggat gcacccttaa tgttttgaat atatcggtgc
     7261 atttcttcaa tctttgagat tctgtctacc ccacctctga gagattctat ggcagattcg
     7321 atatttggaa tgtagctttt agcctcaccc acaaatccag ctataagttg ttgatcgatt
     7381 gatttactca tataattatt agttatttat aattttctat atactaaatt actatttata
     7441 tattcagtct ccatcccatt tatcctagcc ggcaaaatat cagaagggcc aagtattagc
     7501 gagcccccct tgcctaaata ttcacaaagt ctgtatacaa tctccaacct tctgttcttg
     7561 ctgaaataga atagaacatt ttgacaaaaa atgatatcga cgggaaacac tttataagtg
     7621 ttggattcta ctaaattaat aaaattaaaa ctcacaagat cttttacttc atctctaaat
     7681 ttatagtatg ttgtgccgct accgttgtat ggaaataaat acctttctat gtattccggg
     7741 tgcaagctct ttacttctgc ttttgtgtat attcccttcc tggctttttt taacaaatgg
     7801 agattgatat ctgaccctat tatcttagta taatattcgt ctgtatcata gctctgcaaa
     7861 gcattcaggg ctatggaata tgcttcctgg cccgatgagc aacccacact ccagacagtt
     7921 agcagtttga atatatcgtt tttcctactc tctaaaacct gagggaaaat atgctctttt
     7981 agcaactcga atgaaggttg gtgtctgaaa aaacttgtct cacagttcac aagcctgcaa
     8041 tacagttctt tccactccct ttcagcatca ttgttataaa gcaaatacct gagatactca
     8101 tcataccctg taagagaaag atcatttatt ctctcaagca gtgctgtaca tagaaatgaa
     8161 atcctgcttt tatcaaaata caatccgcat tttgattcta tacattgctg ccagcgactg
     8221 aactcttgct cgattagttc atattgctca agcttctgta gacttaaact atcgctgtct
     8281 tcgtatgcag tgctagtatt tatattcatt gtttattaat ttgcccagaa atgcccattg
     8341 gtaattattt attccggtct aattatattc atgaagcatt tgttaaacca aactagcctt
     8401 actgtgttgc cccctcggca ggtgttacag actcggccga aggaatatca acatcagacg
     8461 tagtaaactc cggacttgct ccattggttt ctggcagctt aaacgcactg attgttcttc
     8521 caagttcttc agaaagctct gtcagacccg cagacgtggt aaaagcttca cgtgtactaa
     8581 gtgtagattg ctgcgttacc atggaaatat cagacatagc atttgcaatg gtttctgaac
     8641 ccctggcctg ttgttgagca gccattgaaa ttgactgaat gaggtcagcg agttttacag
     8701 acacagactc aatctcaccc agcgccttac cggcatcagc cgctctttca gatcctgcca
     8761 caacctcgtt tgtcatgtct tccatagctg aaatagcatc gttagattca gcctgaatcg
     8821 ttttaattag agtttcaata cgttttgtgg aatcagtagc acgatctgcc agacgttcaa
     8881 cctcttcagc aaccacggca aaacctctac ctgcttcccc tgccatagca gcctgaattg
     8941 aagcgttaag agcaagaata ctcgttctgt ctgcaatttc accaattagc tgtgtgatct
     9001 ctccaatctc ctgagaactt tcacccagcc tcttaattct ttttgatgtt tcctgcacct
     9061 gatctctgat gttgttcata ccagttatgg tattttccac aacctctgcg ccctgttttg
     9121 cattacttag cgcctgttga gccacagtgg ccgacattga agcgttctct gacacctgtc
     9181 taattgagat tgccatttcg tcaatagcag ccgttgtatc aataatctga cccgactgtt
     9241 cttcactacc ctgagacagc ctgtccatgg aaagcctaat gtcagatgat gcctcattta
     9301 ctcgagtaga tgcagactgc acctcggtaa taatacgcct taactcaaca atcgcattgt
     9361 taaatgagtc agcaatagca ccggttacct ctgcagtaac ctctgcttcc tgtgtaagat
     9421 caccctcagc aataccactc acctcgtcaa gaagcttaat aatggattcc tgtgttctgt
     9481 tatattcatc ttccactctt gctgcatcct ctttaacttt attggttaga gcctcgtttt
     9541 cgtccataaa ctggttaaca gatcctgcca tttcaccaag ttcgtctgaa cttgacatta
     9601 ttgatcttgc agatacatca ccttcagtat atgcacccat agcacttcta ataaagtcga
     9661 tctgtccaag cacacccctt agcaccaata ccgcaataag aagtgacaaa agaatagctg
     9721 caccgattac cagaagaata ataatcttct gatttccaac ctgctgaatc cttgctgaaa
     9781 gtaaattttc aatggcttgg agagctgagt cgtaaggtgc aaagattgaa gcactaacag
     9841 tttcaacctt ttcattaata ctctgtttgg ttgtagttac ggtagctggt cgtacaatat
     9901 cattattaat atcttctaca aaaacggata tatctctttc aagtttaagg ataagtggcc
     9961 tgagctgtga tcttagatct atatttgtag caagataaag ttcagaacct ctttttataa
    10021 tctctaggtt attttctacc agtagtagct gatctgatat ttcatccctc gaaacaccat
    10081 cctgttttaa ctgaacaatt atttcaccat caacaccctc ttgatagaca ttcctgtcac
    10141 tgctctttct tatagcaagc tcagataatc tttcaatatc tttaatatct gtggcaaggt
    10201 taggaagttc tgctaaaaac acatccgcaa tttcgttttt taactgtgaa ctgttaacaa
    10261 tctgaataat aaagtcagat atttcagttt catgctcttt ttgaaccagg taactgtctt
    10321 ctgcgctaag ctcaagagct gttgctatta actgttcgca acctgttttt aagcttcccc
    10381 atacaccggt aagaccaaaa atatcaccat atttattgtt caacgcatca acctgagcta
    10441 ttttatcttc catagacttt ttaagcctgt ctagttcagc ctgggtttgg tcgtcaagtt
    10501 gtctattgtt ttcaagtaca tctattgtaa cggttctaaa atcttcgatt atatctccat
    10561 aatctctaag tggctgtata tattcaatac ctgcaaactg tcttctagcc aggttttcct
    10621 tattatctaa ctcaacttta aggaaataaa gaataaccgg cacagatatg attataggta
    10681 ccaacaccag caaaaccagt ttctgccata atctcatttt tactctcctc ctaacttata
    10741 caaatctatt aactacaagt taaattcgaa ttatttttaa aaacaaatat atattaccca
    10801 caacaatcag agacaaactc tgatgttttt ttaatctaaa ttactttaat tttgtaatct
    10861 aaatacttta attcatgaaa tacttgcact aattataata ccttttcata ttaatgtaca
    10921 tatcctaatt cttaaatttt attaaatgct cttgaccctc acccaaaaca aaagctgtac
    10981 caggataatt agagatttcc ggctttttgt gttctttaac atactcctcc ggcgtcatgt
    11041 tatctagtgc cgaatggggt ctgtactcat tgtaatctct cctccaagtt tctat
//
LOCUS       DBNGSV010000154        11460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1342998, whole genome shotgun sequence.
ACCESSION   DBNGSV010000154 DBNGSV010000000
VERSION     DBNGSV010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1342998"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..819
                     /locus_tag="AC159U_05350"
     CDS             166..819
                     /locus_tag="AC159U_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005802 - trans-Golgi network [Evidence
                     IEA];
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA];
                     GO_process: GO:0034067 - protein localization to Golgi
                     apparatus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GOLPH3/VPS74 family protein"
                     /protein_id="HIR3336515.1"
                     /translation="MLTLAEDILLLLLDDESGELANIDLMTLNYAMAGAVLMDLGLKN
                     RIDNDLEKLIVADSTPTGIAMLDKYLQKINSENNDNNTRFWLTELSNYGEEIMDSAIE
                     MLIDKKILKMEEKKFLWVIGTRVYPMIDDTEEKEVKKRIIDLLMSDDIPSPKDVVLVS
                     LMDTCSLFTTFLSEKEVERLSDRIELIKKLDLIAQEVTNVLERLRSDIAEAMLMLPT"
     gene            863..1192
                     /locus_tag="AC159U_05355"
     CDS             863..1192
                     /locus_tag="AC159U_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3336516.1"
                     /translation="MNLGEEKIGNVLVLKPESRIDSQNSADFEQGVLKLTENEKSILL
                     DFSGVEYISSACLRTLLTLTKKIKEQGGNIAITSLSDNVKQIFKVSGFDSILQIFEDK
                     EQALSSL"
     gene            complement(1193..1600)
                     /locus_tag="AC159U_05360"
     CDS             complement(1193..1600)
                     /locus_tag="AC159U_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336517.1"
                     /translation="MKLDKLINTIKELVDKADKQIYFDKKELYFEINAPETRFSISTK
                     QDGTLCITIHVTDGIFYKFYTERDGTLLLEKGSVPDLIENFGLNYIFKLFDNAAPGML
                     EIGLREIESNDYIFRHTIRAGNKNSVEEKRSLH"
     gene            complement(1779..1922)
                     /locus_tag="AC159U_05365"
     CDS             complement(1779..1922)
                     /locus_tag="AC159U_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336518.1"
                     /translation="MKQIIKTAVSLILIFVIGFTTGIAVNKMQNMHSGYQGNISMDAL
                     SKN"
     gene            2143..2469
                     /locus_tag="AC159U_05370"
     CDS             2143..2469
                     /locus_tag="AC159U_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3336519.1"
                     /translation="MIVIVGGLATVLLTISHLLGHKVYDKIKLSPYECGVNPVGDARE
                     KFSVKYYVIAALFILFDVEILFLFAWAVVYKKLGLIVFLEIVIFFAVILTGYFYMIKR
                     GALKWQ"
     gene            2460..2945
                     /locus_tag="AC159U_05375"
     CDS             2460..2945
                     /locus_tag="AC159U_05375"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3336520.1"
                     /translation="MAVNMNSNLGGDGFLTTTINNVANWGRKNSLWPMPFGTACCAIE
                     MMGVFASRFDLSRFGAEVARFSPRQADLMIVSGTITYKMASVCRRIYDQMPNPKWVIA
                     MGSCTCGGGPFDSYAVVQGIDEFLPVDVYVGGCPPRPEALVDAVIKIQKKIEIEGPPI
                     V"
     gene            2942..3466
                     /locus_tag="AC159U_05380"
     CDS             2942..3466
                     /locus_tag="AC159U_05380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3336521.1"
                     /translation="MSIDFPSLLTSIQTRLDSSVQKIESFRGETTLYITRKSFFKVCR
                     LLKEEYGFTFLVDVTAVDYLDKRDTRYEMVYVLHRFGNDYEENFRLRLKVLLDEKELS
                     IDSITPIWSSANWLEREVYDMFGVQFPGHPDLRRILMPDDYEPHPLRKDFDVRNREPS
                     KQSFEKALKEGIEQ"
     gene            3466..4689
                     /gene="nuoD"
                     /locus_tag="AC159U_05385"
     CDS             3466..4689
                     /gene="nuoD"
                     /locus_tag="AC159U_05385"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR3336522.1"
                     /translation="MERVEFITESPDSQSGLSTTSMMLNMGPQHPSTHGVLRVVLHLD
                     GELVVKAIPHIGYLHRGIEKLCENITYQQCLPYTDRMDYLAAICNNIGYILTVEKLLG
                     IQDEIPERAKVTEVILFELGRIESHLVGIGTHALDLGAMTAFFYCFKERERIYDILEL
                     VCGARLTTSYPRVGGLPLDLPQDFEQKVGNFLKIFPKTIDEVDKLLTRNKIWIQRTKD
                     VAKISAEDAINLGLSGPTLRGSGVPHDLRKSNPYLDYDKYDFEIPVGTDGDAYSRYLC
                     RLEEMRQSVKIIEQALNNLPDGPYVADLPDIVLPEKELTYTKMESLIRHFVLVYEGFK
                     PPAGEVYHGVENPKGELGYYLVSDGTAKPYRMHVRGPSFVNMQAIPQMVKGALLADVI
                     AAIGSIDIVLGEIDR"
     gene            4737..6062
                     /locus_tag="AC159U_05390"
     CDS             4737..6062
                     /locus_tag="AC159U_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR3336523.1"
                     /translation="MLNSKSILFLFILTPFILILLSLNLYAQGKTNLTFDPDDPPETT
                     IPIAPYLTFGAQLDLEYNFFGNIDPENEIEKNLSILEPGLTLAFSFDPTRHIQAFLET
                     SISRQITFTDGDKGEDNLKLEIEQGYLLFKELFDDRLSIQLGRQRFEDEREWLYDEDL
                     DAIRLIYDISDISLELSASRLNIVDRDLLNSDEKERIYNYGFYANYETEHEEKEKILG
                     AYAFLRDDRSQDNESPIFLGIQGEGDLTENLEYWLDTAFVFGESGDNDIRAFGFDIGS
                     IYQFDLPFEPSVTLSYAFGSGDSNPEDGTDSNFRQSGLQSNEGSFNGAADFKYYGEIF
                     DPELSNIHILSAGFGINPTEQSSIDIVYHYYRQHRAADFIRDSALNTDPNGTTKDIGN
                     EFDLVLGYERIRTPKLEAALKLGYLLPGEAFASNSENIFLVKFEIQYEF"
     gene            complement(6082..6738)
                     /gene="lolA"
                     /locus_tag="AC159U_05395"
     CDS             complement(6082..6738)
                     /gene="lolA"
                     /locus_tag="AC159U_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR3336524.1"
                     /translation="MRFLITFLTLLFSITSVVLANGNAQDAVIEKIENKYKKVNTYYA
                     SFRQEQPVTSLDKVQTNVGEIWIKKPGKIRWNYNIPKNAQIISDGNFLWYYYVEEKYA
                     LKRDLKEVGSDTNLLSLLSDLSNLQKLYKINIKRTAIEAVKYYLVELIPLDSEDDSVN
                     KQILAVNMDNLLIERIYIYDAFGNKSTIKFSDIKVNNGIKDKVFEFTPPKDVEIQDAP
                     "
     gene            complement(6735..8966)
                     /locus_tag="AC159U_05400"
     CDS             complement(6735..8966)
                     /locus_tag="AC159U_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR3336525.1"
                     /translation="MGVKIKEPEYKYFILREIFASIFLTLGLFSVISLFLYTSGSGAD
                     LKSSVGTVGLYISNKLGIYFGLSSFIFPIIMIYTSFVVFKNKDMPQLLRKAISGGVFL
                     IALTTFIGLANSSSELLGFSPAGGLLGAKLSSLLRDHLAGTIGSYLIVTLSLIISLIV
                     LINLSVKDLFSFIENTSAFIMNLLSITSKNVYSMVHSSITSRETSKPVLFDDSTPIEK
                     VLNLDEITYKEPVIEPVKESIFPQIVIDRNTTEPKNSVYIPESRSDIDFKLPEADLLD
                     IKEISNIEIDKELIYQNSKLIEEKLKDFGVIGKVTEVRPGPVITMFEYKPAPGIKINK
                     IASLSNDLAMGLSARSIRIIAPIPGKNVIGIEVPNHIREEVTIRELIDDTQFINDESS
                     LTLALGKDIAGHPLFMDLRKAPHLMIAGTTGSGKSVFLHTVITSMLYKSTPKDLKFIM
                     IDPKMLELSVYEDLPHLLHPVVKNPKEAVAALKWAVREMEVRYKLLSEEGARDIQTYN
                     KKIEAYADTEDAPAPLPYLVIIIDELADLMMIAPSDIKESITRLSQMARAAGIHLIVA
                     TQRPSADVVAGLIKANFPARVSFLVSSKIDSRIILDAHGAEQLLGKGDMLFLEPGTSN
                     LIRVQGAFISESESDRVSEFLKSQATPEYNQEITKLQEMSDDENLDDEKDELYLEALK
                     IISETGQASISMLQRRLKIGYNRAARIVEIMEKEGFIGQQESAGKPREVYIDSNSIKE
                     LSY"
     gene            complement(9077..10519)
                     /locus_tag="AC159U_05405"
     CDS             complement(9077..10519)
                     /locus_tag="AC159U_05405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3336526.1"
                     /translation="MEALYISTLNILPELSLIIAGLILLLAGPFLKKEENDAGFFITL
                     IGILIAFYLNFTRFTTPAEAFAGSLSLDSFTAYFNSIFLIGSLISVTFSRDYLKTHDL
                     KASEFYTLILFATSGMMFLGSAREFMSLFLSFEIMSISVYVLTALNRNSLKSTEAGIK
                     YLILGGFSSAILLFGIALVYGATGSIVFSDISAAFDYGNPMLIVGVGLIFIGFLFKIG
                     AFPLHQWVPDIYEGAPVTVTGFMSVGVKAAAFAVLLRVFLEAFPTFEANWLMALWVVA
                     VFTMTIGNVSALMQNNLKRLLAFSSIAHAGYAIIGIASMIGNREYAFASVNYYLLAYM
                     FMNLGAFGILAYLSKKDSECETFDQIAGLWDKKPAIATALTIFMVSLAGIPPFLGFFA
                     KYKIFLSAIEADLIWLAVIGIINSVISAYYYLKVIVYMYMKENKFDFPQLKIASSVAI
                     VLLSLINVFLGIFPLHTWDLALKAANSIPF"
     gene            complement(10647..>11460)
                     /locus_tag="AC159U_05410"
     CDS             complement(10647..>11460)
                     /locus_tag="AC159U_05410"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3336527.1"
                     /translation="SRELNENQIYRYSRNILIPEIGGEGQKKLLDAKVFCVGAGGLGS
                     PAALYLAAAGVGTIGIADSDNVDISNLQRQVIHSTDDIGRTKTSSAKERLNKLNPEIN
                     IIEHSELICEQNIRDIIKDYDVVIDGSDNFATRYIVNDGCFFEKKTLISGAVLQFEGQ
                     VSVFKPHLGGPCYRCLFAEIPPQGVIASCQEAGIIGAVAGIVGSIQSLETIKELLNIG
                     QSLDGRLLVINTLSMEIDELKIKKLGGCKLCGSSPSIDKVNGHPQGPCSGNS"
BASE COUNT         3529 a         2096 c         2258 g         3577 t
ORIGIN      
        1 tccaaaagct ttaatacaag acaaaaaaac cacgaactat gaacaaactt tcactccaac
       61 tcactgacaa aatgttaaca aattctaact ttaaaactgg acctaaattc tggggcttga
      121 caatatccta taattatagt tacataacat atttgggagt aattaatgct tacattagca
      181 gaagatatat tgttgttgtt actcgatgac gaatcgggag agcttgcaaa catagacctt
      241 atgacgctta actatgctat ggcaggtgca gtgcttatgg atctaggttt aaaaaacagg
      301 attgataatg atcttgaaaa actaatagta gccgattcta ctcctacggg tattgcaatg
      361 cttgataaat atctgcaaaa gataaacagt gaaaacaatg ataacaatac acgcttctgg
      421 ttaaccgagc tgtccaatta tggtgaagaa ataatggact cggccattga gatgctcata
      481 gataaaaaaa ttcttaaaat ggaagaaaag aagttcttat gggtgattgg tacaagggtc
      541 tatccaatga tagatgacac agaggaaaaa gaggttaaaa aaaggatcat cgatcttctt
      601 atgagtgatg atattccatc tccaaaggac gtagtattag taagtcttat ggatacatgc
      661 tcactattca ctacttttct cagtgaaaag gaagtcgaaa ggctatcaga tagaattgag
      721 ctcataaaga aattagacct gatagcacaa gaagttacta atgtgctgga aagactaagg
      781 tcagatattg cagaagcaat gcttatgcta cctacataac aatttgttat acataattac
      841 taaaagttaa agaggaatta caatgaatct tggtgaagaa aaaattggca atgtattggt
      901 attaaaacca gaatccagaa tagactctca aaattcagca gattttgaac agggagtgct
      961 aaaacttaca gaaaatgaaa aatctatact gcttgatttc tcaggggttg aatacattag
     1021 cagtgcttgc ctaaggacac ttcttactct tactaaaaag ataaaagagc agggtggtaa
     1081 tatcgccatt acaagccttt ctgataatgt taaacaaata tttaaagtaa gcggttttga
     1141 ttctatacta caaatatttg aagataagga gcaggcgctc tcaagcttat agttagtgta
     1201 gactacgttt ttcctctaca gaatttttat ttccagctct aatagtgtgt ctaaatatat
     1261 aatcattact ttcaatttct ctaaggccaa tttcaagcat accgggtgcg gcattatcaa
     1321 acagtttaaa tatataattt agtccgaagt tctctataag gtctggaaca ctcccttttt
     1381 cgagcagtag tgtaccgtct ctctcggtgt aaaatttgta gaaaataccg tcagttacat
     1441 gaatagttat acaaagtgtg ccgtcttgtt tggtgcttat tgaaaatctg gtttcaggtg
     1501 cattgatttc aaaataaagc tcttttttat caaagtaaat ttgtttatca gctttatcaa
     1561 caagttcttt aattgtgttt atcaatttgt ccagtttcat atttgttcag tctaatcatt
     1621 aagatattgg tggatgggca tgatattact ataacacact gcgatgctat ttataataaa
     1681 ttaaatcgta gtttctatac aatctattaa ttaggctgta tatggaatag gtttaatagt
     1741 tgaattttga gataataaga tagataatat attaaagatt agtttttact caaagcatcc
     1801 atgctgatat tgccttgata cccggaatgc atgttctgca ttttatttac agcaataccc
     1861 gtagtaaatc caattacaaa aataaggatt agtgatacag cagttttaat aatttgtttc
     1921 atcacaatct cctccccgag atctataatg gtaaacagta atactttggt attaagttta
     1981 atctaaccaa atcacttcgt caatcacaat ttattggaag cttttgttta ttattagaat
     2041 aattacgatt attttaaaat taattgtata tataccttga cttttaacgc tttgttttta
     2101 acattagtgt ccatgctgga gggctactta cctatattat taatgattgt tattgtagga
     2161 gggcttgcta ctgttcttct cactatttcc catctgcttg gccacaaggt ctatgacaag
     2221 ataaaactct caccctatga atgcggagta aatcctgtgg gagatgccag ggagaaattt
     2281 tcagtaaagt actatgtaat agctgctctg ttcatacttt ttgatgttga gatattattt
     2341 ctctttgcat gggcagttgt atataaaaaa ctgggtttaa tagtatttct tgaaatcgtt
     2401 atattctttg cagtaatact tacaggttac ttctacatga taaaacgcgg agcacttaaa
     2461 tggcagtaaa catgaactcc aaccttggag gggacggatt tttaactaca actattaata
     2521 atgttgccaa ctggggcagg aagaacagcc tttggcccat gccctttggt acggcttgtt
     2581 gtgcaattga gatgatggga gtatttgctt cgaggttcga tctatcaagg tttggtgctg
     2641 aagtagccag gttttcccca aggcaagccg acctcatgat agtttcaggc acaattacat
     2701 acaaaatggc atcggtatgc agaaggattt acgaccaaat gccaaaccca aagtgggtaa
     2761 ttgcaatggg atcatgtaca tgcggcggcg gtccttttga cagttatgct gtggtgcagg
     2821 ggattgacga atttttaccg gtggatgttt atgtaggagg atgtccacca agacccgagg
     2881 ctcttgtgga tgctgttatt aaaatacaaa agaaaataga aattgaaggt cctcctatag
     2941 tatgagcatt gattttccat ctcttttaac ctctatccaa acacgtcttg attcttctgt
     3001 ccaaaaaatt gaatcattcc gtggagagac aacactatat ataacaagga agagtttttt
     3061 taaagtgtgc agattgttaa aagaagaata tggatttacc ttcttggtag atgttactgc
     3121 tgtagattat ttagataaac gcgatactcg ttacgaaatg gtttatgtac tccacaggtt
     3181 tggaaatgat tatgaggaaa acttcagatt aagattaaaa gtgctgctgg acgaaaaaga
     3241 actttcaatt gattctataa caccaatctg gagcagcgct aactggcttg aaagggaagt
     3301 gtatgacatg tttggtgtgc aatttccagg acaccctgat ctgagaagaa tacttatgcc
     3361 agatgactat gagccacacc cgcttagaaa agattttgat gtaagaaaca gagagccgtc
     3421 aaagcagagt tttgaaaaag cactcaaaga gggtattgaa cagtaatgga aagagttgag
     3481 tttataaccg aatctccgga tagccaaagc ggcttatcca ctacatcaat gatgttaaac
     3541 atgggccctc aacacccgtc tactcacgga gtgctgagag tggtgctgca tcttgacgga
     3601 gagcttgttg taaaggccat tccccatatc ggatacctgc acaggggaat tgaaaaactg
     3661 tgtgaaaaca ttacctacca gcagtgtctg ccttatacgg acagaatgga ctatctggca
     3721 gctatttgta ataacatagg ctatatatta acagtggaaa agctgcttgg aatacaggat
     3781 gaaattcctg agcgtgctaa agtaaccgag gttattctct ttgagcttgg aaggattgag
     3841 tcgcatcttg taggaattgg aacacatgcc cttgatcttg gggccatgac tgcatttttt
     3901 tactgcttta aagaaaggga gagaatatac gacatacttg agcttgtatg cggcgcaagg
     3961 cttactactt cttacccaag ggtaggcgga ctcccgttgg atttaccaca agattttgaa
     4021 caaaaagtcg gtaactttct aaagattttt ccaaagacta ttgacgaggt agacaagctt
     4081 cttaccagaa acaaaatctg gattcaacgt actaaagacg ttgcgaaaat aagtgccgaa
     4141 gacgcaatta atcttggatt atcaggtcct actctaagag gaagcggagt acctcatgac
     4201 cttaggaaat caaaccccta tcttgattat gacaaatatg attttgaaat accagtaggt
     4261 actgacggcg atgcatattc gaggtaccta tgccgcttag aagaaatgag gcagagcgta
     4321 aaaataatag aacaggctct taataacctg ccggacggcc cgtatgtagc tgatcttcct
     4381 gatatagtac tgcctgaaaa agagcttaca tataccaaga tggagtcgct tataagacac
     4441 ttcgttttag tatatgaagg gtttaagccg cctgcaggtg aagtatacca cggtgtggaa
     4501 aacccaaagg gcgagcttgg ttattacctt gtgagtgacg gaacagccaa gccatacagg
     4561 atgcacgtac gaggcccatc gtttgttaac atgcaggcaa tcccgcaaat ggtaaaagga
     4621 gcgcttcttg ccgatgttat agcagcgata ggtagtatag atattgtact tggcgagatt
     4681 gatagataat aaaacacaga tctagtctgt attattacaa ttcaattgga gtacaaatgc
     4741 taaattccaa aagtatattg tttttattca tactgactcc attcatccta atattattat
     4801 cattaaatct atatgctcag ggtaaaacaa atcttacttt cgatcctgat gacccgcctg
     4861 aaacaactat acccattgca ccttacctta cattcggtgc acagcttgac ctggaataca
     4921 acttctttgg taatattgat cctgaaaatg aaatagaaaa aaatctgtct atactcgaac
     4981 caggactaac acttgctttt tcctttgatc cgacaagaca cattcaggcc ttcttggaaa
     5041 catcaatcag caggcagatt acatttactg atggtgataa gggtgaagat aatcttaaac
     5101 tcgaaattga gcagggctat ctgttattta aagaattgtt tgatgacaga ctgtcaatac
     5161 aattaggaag acagcgtttt gaagatgaaa gggagtggct ctacgatgaa gatctggacg
     5221 cgatcagact aatttatgat atatccgata tttctttaga gctttctgcc agcagattaa
     5281 atattgtaga tagggacctt ctaaattctg atgaaaaaga gaggatttat aactacggtt
     5341 tctatgcaaa ttatgaaact gagcatgaag aaaaggagaa gattcttggt gcatatgcct
     5401 ttttgagaga tgacagatct caagataatg aaagtccgat ttttttgggt attcaaggtg
     5461 agggagatct aaccgaaaat ctggaatact ggcttgatac tgcttttgta tttggagaga
     5521 gtggcgataa tgatataaga gcgtttgggt ttgatatagg atcaatctat cagtttgatc
     5581 ttccgtttga accttctgtg accttaagct atgcatttgg aagtggtgac agcaatccgg
     5641 aagacggcac tgacagtaat ttcagacaga gcggactaca gagtaatgag ggtagtttta
     5701 acggtgctgc cgattttaaa tactatggag aaatatttga tccggagcta agtaatattc
     5761 atattctatc tgctggtttt ggaataaatc ccaccgagca gagttctatc gatatcgttt
     5821 atcactatta cagacagcac agggcagcag attttattag ggactcagca ctaaatacag
     5881 atccaaacgg gactactaaa gatattggaa acgaattcga tcttgtttta ggatatgaaa
     5941 gaatccgtac accgaaatta gaggcagcac ttaagcttgg ctatttactc cctggagaag
     6001 cctttgcatc aaattcagaa aatatattct tagtaaagtt tgagattcag tatgagtttt
     6061 agtctttgtc tttgagtttg tttacggcgc atcctgtatc tctacatcct ttggaggcgt
     6121 gaactcaaat actttatcct ttataccatt gttaactttt atatcactaa acttaatagt
     6181 gctcttgtta ccaaaggcat catagatata tattctttca atgagcaggt tatccatatt
     6241 tacagcgagt atctgtttgt ttactgaatc atcctcgctg tctaggggga taagctctac
     6301 aaggtaatac ttaaccgctt caatagcagt tcttttgata ttaattttat ataatttttg
     6361 aagattgctt agatctgaaa gtagtgatag caaatttgtg tcactgccaa cttctttaag
     6421 gtcacgtttt aatgcatatt tttcttccac gtagtagtac cacaggaaat tcccgtcaga
     6481 tattatttgc gcatttttgg gaatgttata gttccatctg attttgccgg gttttttaat
     6541 ccaaatctct ccaacatttg tctgtacctt atcgagagat gttacgggct gctcctgcct
     6601 gaatgaggca tagtaggtat taactttctt atacttgttt tctatctttt caattaccgc
     6661 gtcctgtgcg tttccatttg caagtacaac tgatgtgata ctaaataata atgttagaaa
     6721 tgttatgaga aatctcaata tgatagctcc ttaattgaat ttgaatcgat atatacttct
     6781 cttggtttgc cagccgattc ctgctgtcct ataaaaccct ctttttccat tatttctaca
     6841 attcgtgctg ctctgttata accaattttt agtcttcttt gtaacatgga aatcgatgcc
     6901 tgaccagttt cgctaatgat tttcagtgct tcgagataaa gctcatcttt ttcatcatca
     6961 aggttttcat catctgacat ttcctgaagc tttgtgattt cctgattata ttcaggggtt
     7021 gcctgagatt taaggaattc agataccctg tcgctctcac tctctgagat aaaagctccc
     7081 tgtaccctta taagattaga tgtgccaggc tccaggaaca gcatatcacc ttttccaagg
     7141 agctgttctg caccatgggc atcgagtatg atcctggaat caattttgga tgaaaccaaa
     7201 aacgacactc ttgccggaaa gtttgccttg ataagtcctg caacaacatc tgctgaaggt
     7261 ctttgtgttg ctacaataag atgaatacct gctgctcttg ccatctgtga gagtcttgtg
     7321 attgattctt ttatatcact gggggctatc atcattagat cggcaagctc gtctattatt
     7381 attaccagat agggaagagg agcaggggcg tcctctgtat cggcatatgc ctcgatcttt
     7441 ttattgtacg tctgtatatc tcttgcgcct tcttctgata gaagtttgta ccttacttcc
     7501 atttctctta ctgcccactt aagtgcagca acggcttcct ttgggttttt tacgaccgga
     7561 tgtagcagat gaggcaggtc ttcatatacc gagagctcaa gcatctttgg gtcgatcata
     7621 ataaatttaa gatcctttgg tgtagattta tacagcatgc tagtaattac agtatgaaga
     7681 aaaacactct ttcccgaacc cgtagttcct gcaatcatta agtgtggagc ctttcttagg
     7741 tccataaaga gcggatgtcc ggcaatatct tttccaagtg caagagttag agatgattca
     7801 tcatttataa attgtgtgtc atctataagt tctcttatcg ttacttcttc tcttatatga
     7861 tttggaacct caatccctat tacattcttt ccgggaatag gggctattat tcttatgctt
     7921 cttgcgctaa gtcccattgc gaggtcattt gaaagggaag cgatcttgtt tatcttaatt
     7981 ccgggagccg gtttgtattc gaacattgta ataactggcc ctggtctaac ttcggttact
     8041 tttcctatta ctccaaaatc ttttagtttt tcttcaataa gtttggagtt ttggtatata
     8101 agctctttat caatttctat attggaaatt tctttaatat ccaaaagatc cgcttcgggc
     8161 agcttaaaat caatatctga tctgctctct ggaatataaa cagaattttt aggttcagtt
     8221 gtatttctat caattacgat ctgtggaaat atactttctt taacaggttc tataacaggc
     8281 tctttatatg ttatttcatc cagattaagt accttttcta taggtgttga atcatcgaaa
     8341 agcacgggtt ttgaggtttc tctgctggtt atgcttgagt gaaccatgct gtatacattt
     8401 ttgcttgtta tagatagtag attcattata aatgctgatg tattttcaat aaaactaaac
     8461 aggtctttta cgcttagatt aatcagaact attaaactta tgattaaaga taaagttaca
     8521 atgaggtaag aaccgatagt gccagccagg tggtccctta gaagtgaaga cagcttagct
     8581 cccaataatc ccccagcagg agaaaatccc aaaagctctg aagagctgtt tgcaagtcct
     8641 ataaaagttg ttagtgcaat taaaaatacc ccacctgaaa tcgcttttct caaaagctga
     8701 ggcatatcct tgttcttaaa cactacaaag cttgtataga tcattattat tgggaaaatg
     8761 aaagaagata gaccaaagta gattccaagc ttgttggata tataaagccc aaccgtaccc
     8821 actgagcttt tgaggtcggc ccccgagcct gaagtgtata aaaacaggct gattactgag
     8881 aacaaaccaa gggttaagaa aatagaggca aatatttccc tcaatatgaa atatttatat
     8941 tccggttctt taatttttac acccatcttg tcttaaagta attccttaaa ttgtattttt
     9001 gaattggtaa atatataatt tataacaatt taggcaagtt gtaaataata aataatgtta
     9061 tttattaagt tattacctaa aatgggatgg aatttgcggc ttttagggct aaatcccagg
     9121 tgtgtagggg gaaaatacca agaaacacat ttataaggct tagcagcaca atggccaccg
     9181 aagatgctat ttttagctga gggaagtcaa acttgttttc tttcatatac atatagacta
     9241 ttacttttag gtaataatag gcagatataa cactgtttat tattcctatt acagcaagcc
     9301 agatgagatc tgcttcaatg gctgagagaa aaatcttgta ttttgcaaaa aatccaagaa
     9361 atggcggaat acccgcaaga gataccataa agatcgtaag cgccgtggct atggcaggct
     9421 ttttatccca gagccctgct atctgatcaa atgtctcaca ctcactgtct tttttggata
     9481 gataagcaag aataccaaaa gcgcccaggt tcataaacat atatgctaaa aggtagtagt
     9541 ttacgcttgc aaatgcatat tccctgttac ctatcataga cgctatgcca atgattgcat
     9601 agcccgcatg agcaatacta gaaaaagcaa gcaggcgttt tagattgttc tgcataaggg
     9661 ctgatacgtt tccaattgtc atggtaaaca cagcaacaac ccaaagggcc attagccagt
     9721 ttgcctcaaa tgttggaaat gcttcaagaa ataccctgag cagtacggca aaagccgcag
     9781 cctttacccc tacagacata aacccagtaa ctgttacagg agcgccttca tatatatccg
     9841 gtacccactg gtgcagagga aaagcgccta tcttgaataa aaatcctata aatatgagtc
     9901 caacgccgac tataagcatt gggtttccat aatcaaaggc tgctgatatg tcgctaaaga
     9961 ctattgatcc tgtggctcca tatactaaag ctattccaaa aagaaggatc gcagaggaaa
    10021 atccgccaag aatgagatat ttaatacctg cctcggttga tttaagtgaa ttcctgttaa
    10081 gtgctgttag cacatatact gaaatagaca ttatttcaaa gctaagaaac aggctcataa
    10141 actctctggc agagcctaaa aacatcatcc ctgatgtagc aaacagtatg agggtataaa
    10201 attcgctagc cttaagatcg tgggttttaa gataatccct ggaaaatgta acggatatta
    10261 gagaacctat gagaaagata gaattaaaat aggctgtaaa actgtcaaga gaaagtgagc
    10321 ctgcaaaggc ttctgcagga gtcgtaaatc ttgtaaaatt taaataaaaa gctataagaa
    10381 ttccaattaa tgttatgaaa aatcctgcgt cattttcttc ttttttaaga aatgggcctg
    10441 caaggagcag tatgagtccg gctatgatga gacttagctc cggcagaatg ttaagtgtac
    10501 ttatataaag tgcttccata ttgaaaaatt ccggctaatt aaatgtttat ctatgggttg
    10561 gcagagtatt atcttaaaca gcgaggcttt gtcaagatta acaaagtata atgtttatat
    10621 aatgtagaat aaacccgggg aaaatgttac gaattaccac tgcatgggcc ttgcgggtga
    10681 ccatttactt tatctatcga aggtgaactt ccacaaagct tacatccacc aagctttttg
    10741 atcttaagct catctatttc catggaaagg gtatttatca caagaaggcg gccgtcaagg
    10801 ctctggccaa tatttaagag ttctttaata gtttcaagcg actgtatgct tcccacaatc
    10861 cctgctacag cgccgattat tcctgcctct tggcacgacg cgattacgcc ctgtggtgga
    10921 atctctgcga agaggcacct gtagcacggc ccgcccaggt ggggtttaaa caccgatacc
    10981 tgtccttcaa actgcagtac cgcgccggaa ataagggtct ttttttcaaa aaaacatccg
    11041 tcgtttacta tatatcttgt agcaaaatta tcagagccgt cgattactac atcgtagtct
    11101 tttatgatat cccttatgtt ttgctcacaa ataagctctg aatgctcaat gatattgatt
    11161 tcagggttta gcttgtttag cctctccttt gctgagcttg ttttagtcct gccaatgtcg
    11221 tctgttgagt gaattacctg cctttgcaga ttggatatgt ccacattgtc agagtctgca
    11281 attccaattg tgccaactcc ggcagctgca agataaagcg cagccggtga gccaagcccc
    11341 cctgcgccta cgcaaaatac ttttgcatct aggagtttct tttgaccctc tccgccaatc
    11401 tctggaataa ggatgtttct gctatagcgg taaatctggt tttcgtttag ttcccggctc
//
LOCUS       DBNGSV010000155         7387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_304462, whole genome shotgun sequence.
ACCESSION   DBNGSV010000155 DBNGSV010000000
VERSION     DBNGSV010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_304462"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC159U_05415"
     CDS             <1..186
                     /locus_tag="AC159U_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336528.1"
                     /translation="VIPTFTAASLVIFLMSLFGVVPNMILGIRMDLILLSITTIATVI
                     TLLIRAKTQHKSFVPKI"
     gene            409..810
                     /locus_tag="AC159U_05420"
     CDS             409..810
                     /locus_tag="AC159U_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3336529.1"
                     /translation="MKASEIMTHEVLSVSEDTSVMDAVKIMDKNKIGALIVQTPRPTF
                     GIFTQRDLLSRVVARRKSVKNTKIRDVMTETIKCAQVNDNIDDITRLMYEEDVRYLPV
                     MDGRKLVGIISNDDLFKVVFRSSEGYKEEVL"
     gene            892..1767
                     /gene="dapF"
                     /locus_tag="AC159U_05425"
     CDS             892..1767
                     /gene="dapF"
                     /locus_tag="AC159U_05425"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3336530.1"
                     /translation="MENFVKSHGLGNDYIIVDPEQISFEFTKEVIQLICNRNYGVGSD
                     GILLLTSSDTADFGLRILNPDGSEAEKSGNGIRIFSKFLFDYGYTSKTTFSIETLGGL
                     VKADLEVTDSKVNLITVDMGKASFDSEDIPVVGKKREVVKEEIRVNGDILEFTAVTVG
                     NPHCVIFFDKLDDNLIHKLGPMLENHELFPNRTNVQFAQVVSASEVKALIWERGAGYT
                     LASGSSSCAIASASVKNGLTDKKLSVIMPGGILSIEVNDDWTLRMKGPAEQVFEGTFS
                     QETIKRIQTSSYPKY"
     gene            1787..3022
                     /locus_tag="AC159U_05430"
     CDS             1787..3022
                     /locus_tag="AC159U_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3336531.1"
                     /translation="MQKKLLSAQTILPISSKPIYNSSVYIENGKILEIGPTDSLVSKY
                     KDVRHTDLGKGILLPGFINAHTHLELGWITEHIGGFSNFTEWIKQIISAKKNHAPSDR
                     QIRNSVNNGIRRLIKSGTTTVAEISSFEGADKQPLKNCFLRTIIFKEVFDRDIDQIAD
                     IELEKSVLYEERLFPHAPYSCSPETISEVHKHSLKHGNSYGIHLAESQQESLFINNKK
                     NHFESSIYPLLGKENFPRKQANTSLDYMTKNNFFEGVKTTAIHMVHVLEQELQIIKEN
                     DIGVVICPRSNKFLNVGLPPVKLYKELDRIGLGTDGLSSNLNLDMFEELSFLYLTYCD
                     VIGPEMAKFAIYAATLGGAKALFIEDTVGSIEPGKAADLIFVSPQNYYADPYLSVVSS
                     TRSELQMSMVNGEILFSPI"
     gene            3030..4076
                     /locus_tag="AC159U_05435"
     CDS             3030..4076
                     /locus_tag="AC159U_05435"
                     /EC_number="4.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061929 -
                     gamma-glutamylaminecyclotransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR3336532.1"
                     /translation="MINKLFVYGTLLQGQSRNHLLADWKLINTLETKGTMYDTGLGYP
                     AVDFTDKTNNLFGELYLYEGRNIESIISRIDAEEGTKSDLFERKIIESSGEKFFTYKM
                     GSKLENSRNSFSNISSGNWRHKSITQENISKFAIYFEQAQSTRYRVFAPADASSFVHI
                     KGDKPILVVSSHATAHMREGKLKSQEYYTGAIGVILNSISGVHSLYTTYASEFDSNYC
                     ESTRLKDYINEVVKKYHIKFVLDIHGTGSYRRFDIYPGIGKTGEFLNGNNTLLDSLEN
                     ILKYNNLRQGGSDVFPAYRQHTVSRFANTNLAISSMQIEINYKLRDPENDSNSFEKLI
                     SVLAEYIESVGKLV"
     gene            4132..4650
                     /locus_tag="AC159U_05440"
     CDS             4132..4650
                     /locus_tag="AC159U_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336533.1"
                     /translation="MNRFRLCLCILAIFISGVFTSCDKLDQNKPEDVLKQFLAADLVG
                     DHKKAYSFLSLANKKNKSLEQYVSEKSKHSNSELADLFIENTTHLIENVEIDGDNAKI
                     SVDTTTVNLEFITREIANAGFKAATTGKTFSDIQKDIAEKYKTHKLPLHNYKSNHTLI
                     KEKDGWKVILNF"
     gene            4857..6263
                     /locus_tag="AC159U_05445"
     CDS             4857..6263
                     /locus_tag="AC159U_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336534.1"
                     /translation="MIKKYWNSFTVFTLLLLIITSGFSLQAKEQIAQIPAFLPNKSDV
                     PTTKPMPIDGVWLVSPLGKKIRVQEGRAYALESWVHMFVLKIEPGMVTTKDIKPVAAS
                     KYEGYDLPVGGPSKWEVVDGGDIQANVKGPLGPANFKLIPITLDNKDWYISEMSKAGL
                     DLPDDYDTSPPGYDPAPPGYDPAPPGYDPDPPGYDPGPPGDDPIVDPILPGPPVDKPG
                     IQPIPKPKPLPKPKPIVIPGDITPDCGGDGQKICTTKPAYKVGKAAKLGCPGKNLHFS
                     PRNGGECWSCPKDYKRTANFITSKKACTKKLFKGPYSKATFVRSVWGCPAGQFHIAKD
                     GGTCMVCPKGYKRIHAAGADTLVCKVEKKYKCDGPLVAANLPPEKNPFANIFGFSRTK
                     VCGQKFDIAKEAAKDSVDAAKVAYHMNRLKEDVLKRGQGAFAVYHALKDKNWNSAWQH
                     LQGLDSYQDLLQAAREAE"
     gene            6276..6647
                     /locus_tag="AC159U_05450"
     CDS             6276..6647
                     /locus_tag="AC159U_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336535.1"
                     /translation="MGLGGDVQLILGANTEFGIAIDVYNPANFKGYNSTGFSKGIAAG
                     ADLGVAVGLWRGDLDSLPGPSQGIATSVGATYSGGAGVWYSYYTPDTIGQQYLGLTIT
                     GGGGIGFEVGEYNEVYTIFKD"
     gene            complement(6705..7256)
                     /locus_tag="AC159U_05455"
     CDS             complement(6705..7256)
                     /locus_tag="AC159U_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336536.1"
                     /translation="MLTTGFYLIVPHIIIIFITLFLELHLSSGWNRFYFTKGIALFKR
                     SIPLAYSPNLSSETLSMNFNKESSHAMVFHQLSHEEIAFREEYQSFRLSSITPVIHGL
                     IKYKEGNLEIIGLLNWFPIVSSLVVIIVLVGIAFDKEFLIEFKLSSILFATGAVMFFR
                     DLYEDQKVRYSKIYSVLLELARN"
BASE COUNT         2488 a         1342 c         1475 g         2082 t
ORIGIN      
        1 gtaataccta cttttacagc agctagtctt gtaatatttc ttatgtcact ttttggagta
       61 gttccaaaca tgatattggg aatccggatg gatttgatac tcctttccat taccactatt
      121 gccactgtaa taacacttct tattagggca aagactcaac ataagtcatt tgtgcctaaa
      181 atttaacggc tggcttaaat atctttcaac tgtcctacca ctcttattcg ttgcatacag
      241 tatcttcatt gtgcatactc cacagtcctt catcctggtt tcatagtcca tgttttgtca
      301 gcgtacatca aagatccttt ttatattaaa agttttttaa ttaaagacag aataaatgtt
      361 gtaaaataaa tcaatatgta gataaattcg aaataggggg tatctaatat gaaggcaagt
      421 gaaatcatga cacatgaggt attgtctgta agtgaagata cttcggttat ggacgcggtt
      481 aaaattatgg ataaaaataa gataggcgca cttattgtgc agactccaag acctacgttt
      541 ggaattttta cacaacgtga tttactgagc agggtcgtgg cccgaagaaa aagcgtcaag
      601 aataccaaga taagagatgt tatgacagaa actattaaat gcgcgcaggt aaatgacaac
      661 attgatgata ttactcgttt aatgtacgaa gaagatgtga ggtatcttcc agttatggat
      721 ggcaggaagc ttgtagggat tatatccaat gatgacttgt ttaaagtggt gtttaggtcg
      781 tctgagggct acaaggaaga agttttatag atattaagga ttatcttatt tttgaaatca
      841 tatgaatatg atatataaat agaatttgta atctttaaag gatggattta aatggagaat
      901 tttgtaaagt cacatggact gggaaacgat tatatcattg ttgaccctga acaaatatct
      961 tttgaattca caaaagaagt gatacagctt atttgcaaca gaaactatgg agtaggatcc
     1021 gacggcatac tgcttttgac ctcttcggat acagccgatt ttgggctcag gatattaaac
     1081 cccgacggca gtgaagcaga aaaaagcggt aatggcatta gaatattctc taaattttta
     1141 tttgattatg gatatacaag caagacaacc ttctctattg aaactctggg tggattggta
     1201 aaagcagatt tggaagtaac agacagtaaa gtaaatctta taactgtaga tatgggaaaa
     1261 gcatcttttg atagtgagga tattccggta gttggtaaaa aaagagaggt agtaaaagaa
     1321 gaaataaggg taaatggtga tattctggaa tttactgcag tcacagtagg aaatccccac
     1381 tgcgttatat tttttgataa actcgatgat aatctaatcc acaaactagg ccccatgctt
     1441 gaaaaccatg aactctttcc aaatagaact aatgttcagt ttgcccaggt tgtctcggca
     1501 tctgaagtta aggctcttat atgggaaagg ggtgcgggat atactcttgc atcaggtagc
     1561 agctcttgtg ctattgcaag cgcctcggta aaaaatggat taacagacaa aaaactttca
     1621 gtaattatgc ccggaggtat actcagtatc gaagttaatg acgactggac tctaagaatg
     1681 aaaggcccgg ctgagcaggt ttttgagggc acatttagcc aggagacaat aaaaagaata
     1741 caaacatcaa gttatcccaa atattaaaga actttcataa ttaatcatgc aaaaaaaact
     1801 attatcagct caaactatat taccaataag ttcaaaacct atatacaact cctctgtata
     1861 tatcgaaaac ggaaagattt tagaaatcgg cccaacagat tcattagtgt ccaaatataa
     1921 agacgtaaga catacagacc ttggaaaggg aattcttctt ccaggcttta taaacgccca
     1981 tacccattta gaactgggat ggataacaga acatattggc ggtttcagta attttactga
     2041 atggattaaa cagataattt ctgcaaagaa aaatcatgct ccatctgata gacagattcg
     2101 caactctgtt aataatggaa taagacgtct tattaaatca ggcactacta cagttgctga
     2161 gatatcttct tttgagggag ccgacaaaca accactgaaa aattgcttct taagaacaat
     2221 aattttcaaa gaagtctttg atagagacat agatcaaatt gctgatattg aacttgagaa
     2281 atctgtactc tatgaagaaa gactttttcc tcatgcccca tactcctgct ctcctgagac
     2341 tatatctgaa gtacacaaac acagtttaaa acatggcaac agctatggaa tccacttagc
     2401 cgaaagccag caggaatctc tttttataaa taataaaaaa aatcattttg aaagttcgat
     2461 atatccgctt ctgggcaaag agaacttccc cagaaaacaa gccaatacat ctcttgatta
     2521 catgactaaa aataattttt ttgaaggggt taaaacaaca gcaattcata tggttcatgt
     2581 attggaacag gagctgcaga ttataaaaga aaatgatatt ggagtagtta tctgtccaag
     2641 aagcaataaa tttttaaatg tcggccttcc accggtaaaa ctatataaag agcttgaccg
     2701 aatcgggctt ggcaccgatg gtctttcaag taacctcaat ctggatatgt ttgaagaatt
     2761 aagcttttta taccttacgt actgcgatgt tattgggcca gagatggcca aatttgcaat
     2821 atatgcagca acactgggcg gggcaaaagc tctattcatt gaggatacag ttggaagcat
     2881 tgaaccaggc aaggctgctg atcttatttt tgtaagccca caaaactact atgctgaccc
     2941 ttacctgagt gtggtttcga gtacacgcag tgagctgcag atgtccatgg taaacggaga
     3001 aatccttttt tctccaattt agaaaaaagt tgataaataa actttttgtt tacggaactc
     3061 ttcttcaggg acaatccaga aatcatcttc ttgcagactg gaaactcata aatactctgg
     3121 aaacaaaagg cactatgtat gatactggcc tgggatatcc tgctgtagac ttcactgaca
     3181 aaacaaacaa cttgtttgga gagctatatc tctatgaagg aagaaacatt gaatcaataa
     3241 taagcaggat tgatgcagaa gagggaacta agagcgatct ttttgaaaga aaaattatag
     3301 aaagcagtgg agaaaaattc ttcacttata aaatgggaag caagttagag aattcaagaa
     3361 attctttttc caatatatca tcaggaaact ggagacataa atcgattact caagagaata
     3421 tatcaaaatt tgctatatac tttgagcagg cacaatcaac aagatacaga gtgtttgcgc
     3481 ctgcagatgc tagttcattt gttcatatta aaggtgataa acccattctc gtggtatcct
     3541 cccacgctac agctcacatg cgggagggca agttaaaatc tcaggaatat tataccggag
     3601 caattggcgt gatactcaat tccatctcag gtgttcactc tttatataca acatacgcat
     3661 ctgaatttga ctccaattac tgtgagtcaa cccgactaaa agattacata aacgaagtcg
     3721 ttaaaaaata tcatatcaaa tttgttctgg atattcatgg aaccggttct tataggagat
     3781 ttgatattta tccgggcata ggaaaaaccg gtgaattttt aaatggaaac aatacattgc
     3841 tggatagtct ggaaaatata ttaaaataca ataatttgag acaaggtgga tctgatgtat
     3901 tccctgcata caggcagcat acagtttcaa gatttgcaaa cactaatctt gccatttcat
     3961 ccatgcagat tgagataaac tacaagttga gagacccgga aaatgacagc aatagttttg
     4021 agaaattgat aagcgttctt gcagaatata tcgaaagtgt tggcaagtta gtctaaagtg
     4081 ataatattct atttataaaa ttaacattgt ttcaaacaaa gggagcaagt gatgaataga
     4141 tttagattat gtttatgtat tcttgcaatt tttatatccg gtgtttttac atcttgtgac
     4201 aaactggatc agaacaaacc cgaagatgtg ctaaagcagt tcctggctgc tgacctggtt
     4261 ggtgatcaca aaaaagccta ttccttttta tcgttggcaa acaagaagaa caaatcactg
     4321 gaacagtacg taagcgaaaa aagcaaacac tcgaactcag agcttgcaga tctattcatt
     4381 gaaaacacta ctcatttaat agagaacgtg gaaatagacg gtgataatgc aaaaatcagt
     4441 gttgatacta caacagtcaa cctggaattc attacaaggg aaatcgcaaa tgccggcttt
     4501 aaagcagcca ctacaggcaa aacatttagt gacatacaaa aagatatagc cgaaaaatat
     4561 aaaacgcaca aactaccgct acacaactac aaatccaatc atactcttat taaagagaaa
     4621 gatggttgga aagtgatctt gaatttttag gataatacta caactaccta ccggcaaagg
     4681 cattccagga ttatttgata ataaaattcc gttgagaatt gcaagcaaat ttgttacaat
     4741 cgttacgcga tttaactggc aaactatatt cgtacaataa atttatataa gttgtatctt
     4801 aatagatttt tatcagttta aacaaatcag caatttatct gaataggagg atgtagatga
     4861 ttaagaaata ttggaatagc tttactgtgt tcactctctt actgcttata attacaagtg
     4921 gattttcatt acaagccaaa gaacagatag cacaaatacc ggcatttctg cccaataaat
     4981 cagatgtgcc cacaacaaag cccatgccca tagacggcgt gtggctggta tcacctcttg
     5041 ggaaaaaaat aagggttcaa gaaggaaggg catatgctct ggaatcctgg gtacacatgt
     5101 ttgtgctaaa gatcgagccc ggcatggtaa ctacaaaaga tataaagcca gtagctgcca
     5161 gcaaatatga gggttatgac cttcccgttg gggggccttc aaaatgggag gttgtagacg
     5221 ggggagatat ccaagcaaat gttaaaggac ctttaggccc tgcaaatttt aaacttattc
     5281 caattacact tgataacaag gattggtata tatcggagat gagtaaggca ggactagatc
     5341 tgcccgatga ttatgataca tctcctccgg gatatgaccc agccccaccg ggttacgatc
     5401 ctgctccacc cggatatgac ccagacccac cagggtatga tcctggaccg ccgggcgacg
     5461 acccaattgt tgatcctata cttcccgggc ctccggtaga taaacctggt atacagccta
     5521 tacccaaacc aaagcctctt cctaaaccaa aaccaatcgt tataccggga gacattacac
     5581 cggactgcgg tggtgatgga cagaaaatat gtacgactaa acctgcatat aaagtaggaa
     5641 aagcagcaaa acttggctgc ccgggaaaaa atctgcattt ttcaccaaga aacggcggag
     5701 agtgctggtc gtgtcctaag gattataaaa gaacggccaa tttcataact tctaaaaaag
     5761 catgtacaaa gaagcttttt aaggggcctt attccaaagc tacgtttgta agatcagttt
     5821 ggggttgccc ggctggacag tttcatatcg ccaaagacgg cggtacatgt atggtatgtc
     5881 ccaaaggata taaacgtatt cacgcggcag gggcagatac tcttgtatgt aaggttgaaa
     5941 aaaagtataa atgcgacggg ccgcttgttg cagcaaatct tccacccgaa aagaacccct
     6001 ttgcaaatat attcggcttt tcaagaacca aggtatgcgg ccagaagttt gatattgcaa
     6061 aagaagctgc aaaagactca gtagatgcag ctaaggttgc atatcatatg aaccgcctta
     6121 aagaagatgt tttaaaacgt ggccagggcg cattcgcagt atatcatgca cttaaagaca
     6181 agaactggaa ttctgcctgg cagcatcttc agggtctgga cagttatcag gatctgcttc
     6241 aggcagccag agaagcagaa tagagatcga ttacagtggg tctaggcggt gatgtacagc
     6301 ttattttagg tgcaaacaca gaatttggaa tagcaattga tgtttataat cccgctaatt
     6361 tcaagggata taacagtaca ggtttttcaa aaggtatagc cgcaggcgca gatcttggcg
     6421 tggcagtagg gctttggcgc ggtgatttag actctcttcc cggcccttca cagggtatag
     6481 caacatctgt tggagctact tatagcggcg gtgctggcgt atggtacagt tactacactc
     6541 ctgacactat tggccagcag taccttgggc tcaccataac cggcggtgga ggtattggtt
     6601 ttgaagtcgg tgagtacaac gaggtctata cgatatttaa agattaacaa cagttttaat
     6661 aattaacaac caggccaggc cataatatat ttttatggtc tggcctagtt tctggcaagt
     6721 tccaaaagta cgctgtagat tttactgtac ctcacttttt gatcttcata tagatcacgg
     6781 aaaaacatta cagctcctgt agcaaacaaa atggaggata gcttaaactc aatcaagaat
     6841 tctttatcaa aagcaattcc tactagcaca ataattacta ccaatgaact aactatagga
     6901 aaccagttta gtaaaccgat tatttcgaga ttgccttcct tatacttaat taatccgtgt
     6961 attacgggtg taatacttga taatcgaaaa ctttgatact cttctctaaa ggcgatctcc
     7021 tcgtgtgaca attgatgaaa taccattgca tgagaagatt ccttgttaaa attcatgctc
     7081 aatgtttcgg aagatagatt gggtgaataa gcaagtggga tagatctttt aaataacgct
     7141 attccttttg tgaaataaaa tctattccag ccactgctta agtgaagctc caagaacaaa
     7201 gtgataaata taattattat atgtggtact attaaataga agcctgtagt taacatttga
     7261 aaaagtataa catacctgcc cctatgttag aatcacccct caagaatatc gagcttaaca
     7321 tctttggtaa caattccctt ctctcttgcc tttgaaaaaa gaagttcgag tgcttgcctg
     7381 cctttgt
//
LOCUS       DBNGSV010000156         7537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1343408, whole genome shotgun sequence.
ACCESSION   DBNGSV010000156 DBNGSV010000000
VERSION     DBNGSV010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1343408"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1525
                     /locus_tag="AC159U_05460"
     CDS             392..1525
                     /locus_tag="AC159U_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336537.1"
                     /translation="MFLLAIRQLQNEPIRTLLTGLAISTVIAVILILHGFEKGLYVQL
                     ESAVLDRGGDLIVTQSGIKNFLAVRSSLRQFSRREVESIPGVVNAHPITTLLAIYEQN
                     NNKTPIAIIVYDTLGGPTNIKSGSFIEKGNDIIIDLSLAKEYNLKPGDEFVVYDFLFR
                     VSGITKNSAAFFTPLVFISYDGLIDFFIESEIAPDITTFPLLSFLLVELKPPASPENV
                     SNLIENKIGDVDVFTPQQLANNDVKLGKDLFGPVMVLLISIAYVIGLLVVGLIIYSDV
                     RGRLRTLGVLKALGFSMSYISAAVIIQNLMLLIIAFPLGILLSMLISNFIEFNMPLYL
                     VPVLDTGGLSVTLLASVLFTILSSVLPLRFIAKTDPTIAFQGN"
     gene            1529..2638
                     /locus_tag="AC159U_05465"
     CDS             1529..2638
                     /locus_tag="AC159U_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336538.1"
                     /translation="MIHWTIKYLWIQKGRLLTSSACVAGALILVLFFDAVFLGESKQI
                     VAYVRHTSPDIWVMQKGVSNMHMASSFIWDTKADKIAKISGIRDVTPILYLNSMVHAG
                     GHDWFSYIVGLESENQRAGPWDMARGKSNPETGEIILPVIVSNLTRIEIGDTASIADK
                     KFKVVGFSNNTFSMANSIAFVNFTDLQDILSATGTISYLLVDVEQGKDPSKLAEKIKD
                     EVEKVSVLTQREFIKNDFNMAMKMGVEIISIMTLIGSALAVIIIAFTAYTQVASIKHE
                     LAVAKALGVKNHSIYTSVVIQTIIITAIGYLIAIILALFVIPGIIHLAPQFSFLVSAA
                     MIIHIGIIALIVALIAAMIPTYMVIKVDPITAFKV"
     gene            2654..3496
                     /locus_tag="AC159U_05470"
     CDS             2654..3496
                     /locus_tag="AC159U_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3336539.1"
                     /translation="MLLQAKSISKEFGSGHTKVIAVNNVSMDVQAGELVLIMGPSGSG
                     KTTLLSILGTLLKPTNGEVFIDGINTTSMNEAELPNTRAHKVGFVFQAFNLLEALNVE
                     ENILFPAQLIPDKIKDAKARCDELIDRLNLTERHHALPKTLSGGEKQRVAIARALINK
                     PKLILADEPTGNLDSQSGQDVMMILHDITRDEECAILLVTHDPRVEDIADRILWLEDG
                     KLRDRRSEQHHWVKDPVCGMRVDEWTAVSFTEYKDNRYVFCSKRCLERFLDNPGKYHK
                     HESS"
     gene            complement(3519..4394)
                     /locus_tag="AC159U_05475"
     CDS             complement(3519..4394)
                     /locus_tag="AC159U_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3336540.1"
                     /translation="METILFALPNNETIGKKLSERTGLPLGKSEIRQFPDGETYVRID
                     SDVRDKRVVLVCTLDRPDEKLLPLYFLSKTTRDLGANCTCLVAPYLAYMRQDKRFRSG
                     EGITSEYFAALLSSFADSLITIDPHLHRRSSLSEIYSIPTTVSHASAHISNWIKENVG
                     QPVLVGPDKESEQWVSEVADNAGVPHIILEKVRHGDRDVEISVPDIKKHKHRTPVLVD
                     DIISTGRTMIETISHLKNTGMRPPVCVGVHAIFVGDANEDLINAGAERVVTCNTVVHQ
                     SNEIDVSDLLTGFVK"
     gene            complement(4366..5877)
                     /locus_tag="AC159U_05480"
     CDS             complement(4366..5877)
                     /locus_tag="AC159U_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HIR3336541.1"
                     /translation="MKKNISKLRVKSLGIETQHEHIVFMRSDCHVCISEGFEALTRVK
                     VSSESKSIVASLNVITSNHLLKEGEIGFSNSAAKSLRVKNGDLVEVTHLPPIHSLSSV
                     RSKIYGNKLDSTQMKAIIKDIVKGNYSNVHLAAFITACVGTNLEIDEIINLTQAMIDS
                     GKQIRWESDKVVDKHCIGGLPGNRTTPIVVAIIAAADLVIPKTSSRAITSPAGTADMM
                     ETMAPVNLSMRQIKRVVNEEGGCIVWGGIANLSPADDVLIRVERSLDLDSEGQLVASV
                     LSKKVAAGSTHVVIDIPVGKTAKVRTEEQAEKLKYYFNVVGKAIGLELKVLMTNGTQP
                     VGRGIGPALEAMDVLSVLRNEEDAPQDLKERSIQIAGELLELSSRVDSGEGEKLAEEI
                     VNSNKAYDKFISICQAQGGFKEPQYALYEKKVLADQSGIVAEIDNRKIAKIAKLAGAP
                     QDASAGVVFESPIGKIVERGQPLFTVYAQSEGELNYALSYLDSVDGNNIIRIT"
     gene            complement(5895..7253)
                     /locus_tag="AC159U_05485"
     CDS             complement(5895..7253)
                     /locus_tag="AC159U_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR3336542.1"
                     /translation="MKLHFLGAAETVTGSKFLIESGNIQVLIDCGLFQGMKSLRSLNW
                     QPLPINLNKLNAVILTHAHIDHTGYLPLLIKNGFEAPVYSTPATKDLCDILLPDSGYL
                     QEEDAKYANKKGFSKHKPALPLYTKEDAISCLGSFKTIPFNQDFLIQDRLRIRFSDAG
                     HILGAACVHISDGKNKIVFSGDLGCMNDSIMKSPETIESADYLVVESTYGNRIHDNTD
                     PKQELSEIINRTIDRKGILLIPSFAIGRAQTILHLIAELRKEKEIGEIPVYLNSPMAI
                     NATDIFCKYQNEHLLTQDQCHDMCNIAEYVRTSEQSKSLTLKKEPMIIISASGMATGG
                     RILHHLKALVSDPKNTILFIGYQAAGTRGQAMISGVDKIKIHGNYYPVRADVVSINGL
                     SAHADYKDISDWISRMKNHPKKIFLVHGEPSAQEYLKSYLGQQIKSEVIIPQYMQCFD
                     IN"
     gene            complement(7329..>7537)
                     /locus_tag="AC159U_05490"
     CDS             complement(7329..>7537)
                     /locus_tag="AC159U_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336543.1"
                     /translation="LHELLYQIINPKTMSKHVLQLNDKISANSNSKGGPEFWGLTMIR
                     KFYINIYVIRSRIIGNVEVSVLIV"
BASE COUNT         2289 a         1513 c         1346 g         2389 t
ORIGIN      
        1 tccaaaagct ttaatacaag acaaaaaaac cacgaactat gaacaaactt tcactccaac
       61 tcactgacaa aatgttaaca aattctaact ttaaaactgg acctaaattc tggggcttga
      121 caatatcata atattatgat acataaattt aaccattatt gcaactttat cttaaataaa
      181 ccaaaataag aattagatgc ccatatcaaa actatataga agaaactaaa ttcaaaacaa
      241 aatataagga gccatataac aactttcata ttatcaacct caaaccattt tttcttgaac
      301 tttagaaaaa gaactactta ctagaccatt ggaataaagt cctcaaacca agtaatataa
      361 aagctataga tatggagaat ttgtttatgc catgtttctc ttagctattc gacaattaca
      421 gaatgaacca atacgcactt tattaaccgg attggcaatt agtactgtta tagctgtaat
      481 actaatacta catggattcg aaaaggggct ttatgtacaa ttggaatctg ctgtcttaga
      541 ccgtggtggg gatctgattg ttactcaatc aggtataaag aatttccttg cagtacgttc
      601 atcacttaga cagttctcac gcagagaagt cgaatcgatt ccaggggttg tcaatgccca
      661 tcctataacg accctacttg caatttacga gcaaaataac aacaaaacac caattgctat
      721 aatagtttat gatactttag gcggcccaac aaatattaaa tcagggtcat ttatcgaaaa
      781 aggaaatgat ataatcatcg acctgtcttt agcaaaagaa tataatctca aaccaggtga
      841 tgaatttgtt gtatatgatt ttttatttcg agtatccggt ataacaaaaa actctgcagc
      901 attctttaca ccgcttgttt ttatcagtta tgacgggtta attgacttct ttatagaatc
      961 agaaatagcc ccagatataa ccactttccc cttactaagc tttctactgg tagaactaaa
     1021 gcctcctgct tcgcctgaaa atgttagcaa cttgatcgaa aataaaatag gagatgttga
     1081 tgttttcacc ccacagcaac tggcaaataa tgatgtgaag cttggtaagg atttattcgg
     1141 tccagtgatg gttttattga ttagcattgc ttatgtaata ggcttgcttg ttgttgggtt
     1201 aattatttat tctgatgttc gtggtcgttt acgcacactc ggtgtattaa aagcattggg
     1261 gtttagcatg agttacatat cagctgctgt catcattcag aacctaatgt tgttaattat
     1321 agcttttcca ctcggtattt tgttatcaat gctgataagt aattttatag aatttaatat
     1381 gccgctttat ctcgtaccgg tactggatac cggtggttta tcagtaacgt tgctggcaag
     1441 tgtattattt acaatattaa gcagcgttct acctttgcgg ttcattgcga aaactgatcc
     1501 cacaatagca tttcaaggta actgaatcat gatacattgg acaataaaat atctttggat
     1561 acaaaagggc agactgctga caagttctgc atgcgttgcg ggggctttaa ttcttgtcct
     1621 ttttttcgac gcagtatttt tgggagaatc aaaacaaatt gttgcctatg ttcgccacac
     1681 aagtcccgat atttgggtca tgcagaaagg tgtttctaat atgcacatgg caagctcatt
     1741 catctgggat actaaggccg ataaaattgc aaaaatctct ggcataagag atgtcacacc
     1801 aattttatat ttaaattcca tggtacacgc tgggggtcat gactggttct catatattgt
     1861 tggcctggaa tcagaaaatc aaagggcagg cccctgggat atggcaagag gcaaatcaaa
     1921 tccggagact ggagaaatca tactgccagt tatagtatca aatttaacaa gaatcgaaat
     1981 aggtgataca gcttccatag cagacaaaaa atttaaagta gtcggatttt caaataatac
     2041 cttttctatg gccaattcaa ttgcatttgt aaacttcact gatctacaag acatattatc
     2101 agctactgga actataagtt atctattagt ggatgttgag cagggcaaag atccttcgaa
     2161 acttgccgaa aaaattaaag atgaagttga aaaagtttct gtgttgacgc agagagaatt
     2221 tattaaaaat gattttaata tggcaatgaa aatgggtgtg gaaataattt caattatgac
     2281 cctaatcgga tcggcattag ctgttattat tattgctttc acggcttaca ctcaagtggc
     2341 aagtatcaaa catgaattag ctgttgccaa agcactcgga gtaaaaaatc attctattta
     2401 tacaagtgtg gttatacaaa ccattattat cactgcaatc ggttatctta ttgcaataat
     2461 attagcatta ttcgttatcc caggcattat tcatttagcc ccacagttta gctttctcgt
     2521 ttcagccgca atgataattc acattggtat tatcgcttta attgtggcat tgattgcagc
     2581 aatgatccct acctacatgg tcataaaagt agatccaata acagcattta aagtatgaaa
     2641 aatgacaaaa cagatgttat tacaagctaa aagcataagt aaagaattcg ggtcgggaca
     2701 tactaaagta attgcagtta acaatgtctc tatggatgtt caagctggag aattagtgtt
     2761 aataatggga ccttccggtt ctggcaagac aacactactt tccatactcg gaaccttatt
     2821 aaaacctaca aatggagaag ttttcatcga tggaattaac actacatcga tgaatgaggc
     2881 agaactgccc aatacacgag cgcacaaagt cggcttcgta tttcaagcgt ttaatctatt
     2941 agaagcttta aacgttgaag aaaacatctt gtttcctgcc cagttgatac cagacaagat
     3001 taaagatgct aaagctcgct gtgatgaatt aatcgacagg ttgaatctta ccgaaaggca
     3061 ccacgcactg ccaaaaacac tttccggggg agaaaaacag cgcgtagcca ttgcgcgtgc
     3121 attaattaac aaacctaaat taatactcgc cgatgaaccc acaggaaacc tagactcgca
     3181 aagcggccag gacgtgatga tgatactaca tgacattact cgtgatgaag aatgtgctat
     3241 tttgttggtc actcatgacc cacgagtcga agatatagca gaccgcattt tatggctgga
     3301 agatggcaag ctccgcgatc gtaggtcaga acaacaccac tgggtaaaag acccggtatg
     3361 tggtatgcgt gtagacgaat ggactgctgt atcatttacc gaatataaag ataatcgcta
     3421 tgtattttgc tcaaaacgat gtttagaacg atttttagat aatccgggga aataccacaa
     3481 acatgaaagc agttagtagc catgcttata aaattttatc attttacaaa tccggttagc
     3541 aagtcactca cgtcaatttc attactttga tggacaaccg tgttgcaggt gactactctt
     3601 tcggcaccgg catttattaa atcctcattt gcatctccaa caaagattgc atgtactcct
     3661 acgcacacag gcggtcgcat acctgtattt ttcaagtgac taatcgtttc aatcatagtc
     3721 cttcccgtcg aaattatatc atcgactaat accggagtac gatgtttatg tttttttata
     3781 tccggaactg aaatttctac gtccctgtca ccgtgtctta ctttttctaa aattatatgt
     3841 ggtactcctg cattatcagc aacttcagaa acccactgct cgctttcctt atcaggccct
     3901 acaagcacag gctgtcctac attttccttt atccagttag atatatgtgc tgaagcatgc
     3961 gaaacagtag taggaatcga ataaatctct gataagctgc tccttctatg caagtgggga
     4021 tcaattgtta tgagggaatc agcaaaactt gataataaag ccgcgaaata ttcagaggtt
     4081 attccctccc ccgacctaaa gcgtttatcc tgacgcatat acgccagata cggagctacc
     4141 agacaagtac agtttgcacc caaatccctg gttgtttttg ataggaaata aagaggcaaa
     4201 agcttttcat cgggtctgtc taaggtacaa accaagacaa cccttttgtc cctgacatcg
     4261 ctgtctatcc ttacataggt ctcaccgtct gggaactggc gtatttcaga tttacccaat
     4321 ggcaaaccag ttcgttcaga cagctttttc ccgatagttt cgttattagg taatgcgaat
     4381 aatattgttt ccatctacag aatctaaata actaagagca tagttcagtt caccctctga
     4441 ctgagcataa actgtaaaca aaggctgtcc cctttctact attttaccaa tgggcgattc
     4501 gaacactaca cccgcagaag catcttgtgg agcaccagcc aatttagcaa ttttggcaat
     4561 cttcctatta tcaatttctg cgactattcc agactgatcg gcaagcacct ttttttcgta
     4621 taaagcatat tgaggttctt tgaaaccacc ctgtgcttga caaatagata tgaacttatc
     4681 ataagcttta ttagaattta ctatctcttc tgccaacttc tcaccttcgc cgctgtctac
     4741 cctggatgac agttcaagaa gttcacctgc aatttgtatt gatctctctt ttaaatcctg
     4801 aggggcatct tcttcgttcc tgagaacaga aagtacgtcc atcgcttcaa gcgccggtcc
     4861 gattcctctg cccacgggtt gcgtgccatt ggtcattaac acttttaatt cgagtccaat
     4921 ggctttccca accacgttga aataatattt tagtttttca gcctgctctt ccgttctgac
     4981 tttagctgtt ttgccgactg gaatatctat tactacatgt gtagaacccg ctgccacctt
     5041 cttagacagt accgaagcaa ccagctgtcc ttcactatcc aaatcaagtg atctttcgac
     5101 ccttataaga acatcgtctg cgggacttag attggctatt cctccccaaa caatacatcc
     5161 gccttcctca ttaaccactc tttttatttg tctcatagaa agattaacag gagccatcgt
     5221 ttccatcata tcagcagtgc cagcaggcga agttattgca cgggaagaag tctttgggat
     5281 taccaaatca gcggcagcta taattgctac cacaatcgga gtagtccgat tccctggcaa
     5341 accaccgata caatgcttgt ctactacttt atcgctttcc caacgaattt gcttgccact
     5401 gtctatcatg gcctgtgtaa ggttgataat ttcatcaatt tctaaattcg tgcctacgca
     5461 tgctgtaatg aaagctgcaa gatggacgtt ggaataattt ccttttacaa tatcttttat
     5521 aatggccttc atttgagtgc tatccagctt gtttccataa atcttcgacc gaacactact
     5581 aagcgaatga attgggggta gatgggtaac ctcaacgaga tcaccatttt tgacccttaa
     5641 agattttgca gcgctgtttg agaaacctat ttccccttct ttgagtaaat gattagatgt
     5701 aattacattt aaacttgcaa caatagattt tgactcactt gatactttta cacgagtgag
     5761 tgcttcaaat ccctctgaaa tacatacatg acagtcgctt cgcataaaca caatatgctc
     5821 gtgttgtgtt tctattccaa gactttttac tcttaattta cttatatttt ttttcattag
     5881 ttaaaatttg atgattagtt aatgtcaaaa cattgcatgt attgaggtat tattacctca
     5941 ctttttattt gctgtcctag ataacttttt agatattctt gagccgaggg ttccccatga
     6001 acaagaaaaa tcttttttgg atgattcttc attcttgata tccaatccga tatgtcttta
     6061 tagtcggcat gagcagaaag accattaatt gaaactacat ctgcacgaac agggtaatag
     6121 tttccatgaa tttttatttt atctacccct gatatcatcg cttgtcctcg agtaccggca
     6181 gcctgataac cgataaaaag tatagtattt ttagggtccg aaaccaatgc ttttaagtga
     6241 tggagtattc tacctcccgt tgccattcca cttgcagata tgattatcat aggttctttt
     6301 ttcaaagtaa gcgactttga ctgttcagaa gttctgacgt attctgcaat attacacata
     6361 tcatgacatt ggtcttgtgt aagtaagtgt tcattctgat atttacaaaa aatgtctgtt
     6421 gcattaatag ccatgggact atttaaataa accggtattt caccaatttc tttttctttc
     6481 ctcaattccg cgattagatg taaaattgtt tgagcacgac caatggcaaa agaaggtata
     6541 agaagtatac cttttctgtc gatcgtacga ttgataattt ccgatagctc ttgttttggg
     6601 tctgtgttgt cgtgtatgcg attcccatag gtcgattcta ctactaaata atcagctgat
     6661 tctattgttt cgggtgattt cattattgaa tcattcatac atcctaagtc accgctaaag
     6721 acaatcttat tcttgccgtc ggagatatgc acgcaggcag cccctaagat atgtccggcg
     6781 tctgaaaagc gaatcctcaa cctatcttgg atcaagaagt cttgattaaa aggaatagtt
     6841 ttgaacgacc caagacaaga tatggcatct tcttttgtgt atagaggcag ggcaggttta
     6901 tgtttgctga accccttttt atttgcatat ttcgcatctt cttcttgaag gtatccagaa
     6961 tcaggtagca atatatcaca caaatcttta gtcgcaggag tcgaataaac aggagcctca
     7021 aatccgtttt ttataagcaa cggtaaataa cctgtatgat ctatatgtgc atgggttaaa
     7081 ataactgcat taagtttgtt caggttaatt ggcaggggtt gccaattaag tgatctcaga
     7141 cttttcatcc cctgaaatag accgcaatcg ataagaacct gaatgtttcc tgattcaatt
     7201 aaaaatttcg aacctgttac tgtttcagcc gcacccaaga aatgaagttt cataatgatt
     7261 tcatttaatt ctgtaatata ttcgaaactc gatttgtttc cagcttagat aaatatacag
     7321 gtctattttt atacaattaa aacgctgact tctacattcc ctataattct acttcttatt
     7381 acatatatgt ttatataaaa ctttcttatc attgtcaagc cccagaattc aggtccacct
     7441 ttagagttag aatttgctga tattttgtca ttgagttgaa gtacatgttt gctcatagtt
     7501 tttggattta ttatctgata taacagctcg tggagca
//
LOCUS       DBNGSV010000157         2762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1343440, whole genome shotgun sequence.
ACCESSION   DBNGSV010000157 DBNGSV010000000
VERSION     DBNGSV010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1343440"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="AC159U_05495"
     CDS             complement(<1..405)
                     /locus_tag="AC159U_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3336544.1"
                     /translation="MVVTIKKATLDKIIEHSESGFPHEVCGALIGDGKSITEFKICTN
                     LNTERAHDRYELDPESFNDADKWARDNGLEIMGIYHTHPDHPSRPSEFDRERAWPDWF
                     YIILSIMSGKFDNFRTWVLEDFDSQFVEYKLEV"
     gene            complement(396..1343)
                     /locus_tag="AC159U_05500"
     CDS             complement(396..1343)
                     /locus_tag="AC159U_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3336545.1"
                     /translation="MSTSDQAIQYPPENKNAVHNVIELIGNTPLIRLTNYEKQLAPGV
                     ELYIKAEWFNPGGSVKDRPALKMIVDGIEKGKLTKDKIIMDSSSGNTAIAYAMIGAAL
                     GYKVELVIPENVNEERKKILKAFGADIIYSNPLESSDGAIMLARKLFKENPDKYFMPD
                     QYNNPCNPQAHIETTAPEIWNQTEGRVTHFLAAIGTSGTIMGTGQGLKNISKDINILA
                     VEPEESLHGLEGMKHMATSIVPGIYSEAFPDEIINVSTENAYEKMKELLDTEGIFVGH
                     SGGAVMHAALQRAKTLKTGCVVALMPDSGTRYLSGGLWW"
     gene            complement(1343..1570)
                     /locus_tag="AC159U_05505"
     CDS             complement(1343..1570)
                     /locus_tag="AC159U_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3336546.1"
                     /translation="MIDERIDITNEICPMTFVKTKLKLETMGAGQVLEVTLREGEPLM
                     NVPRSAEEDGHKVVSIDKSEQDFYKVIIEKH"
     gene            1707..1955
                     /locus_tag="AC159U_05510"
     CDS             1707..1955
                     /locus_tag="AC159U_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336547.1"
                     /translation="MVEYLTVYVELLNEGVPCRRPVPACEVEPGVYILHKTYSYHPDD
                     EEWEFLPGDKVSCEERISNDGKKILVAITLVEKRKREW"
     gene            2049..2486
                     /locus_tag="AC159U_05515"
     CDS             2049..2486
                     /locus_tag="AC159U_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336548.1"
                     /translation="MGNIFKTGAKINLGIISIPILAMVASFIGLKMWEASCESEFWKQ
                     YYSPDKEWKVVLTKTDCSPFNELSYEGDICRVYYVQNCSNKNIFSFYATDQLDNLRNA
                     SDLLEIVWNGNSSVSVMYDSRLYFDDRHSVHKGIKITYHKKEE"
BASE COUNT          760 a          541 c          544 g          917 t
ORIGIN      
        1 aacctctaat ttgtattcaa caaactgtga gtcaaagtct tccagaaccc atgttctaaa
       61 gttatcgaac tttccgctca ttatggaaag gatgatataa aaccagtccg gccaggcgcg
      121 ttctctgtca aactcagacg gacgggaagg gtggtcgggg tgagtatggt atatccccat
      181 gatctcaagg ccgttgtccc ttgcccattt atcagcatca ttaaatgatt cggggtcaag
      241 ctcataacgg tcatgggccc tttcagtatt gaggttagta cagattttaa actctgtaat
      301 gctcttgcca tctcctatca atgctccgca aacctcatgc gggaatccgc tttcagagtg
      361 ttctattatt ttgtctaacg tagctttttt tattgttacc accatagtcc tccgcttaaa
      421 tatcttgttc cgctgtcggg cattagagcc actacgcagc ctgtctttaa agttttagcg
      481 cgctgcaatg ctgcatgcat cacggctccc cccgaatgtc ctacgaatat tccttctgta
      541 tcaagaagct ccttcatctt ttcatatgca ttttctgtgc tcacgtttat tatttcatca
      601 ggaaatgctt cactgtaaat cccaggcact atggacgttg ccatgtgctt catgccttcc
      661 agtccgtgaa gggattcctc gggctctaca gccaaaatgt ttatatcttt gctaatattt
      721 ttaagtccct gtcctgtccc cattatagtg ccgcttgtgc caatcgctgc aagaaagtga
      781 gtaaccctgc cttctgtctg attccagatt tcgggagccg tggtctcaat atgagcctga
      841 gggttgcagg ggttattata ctgatcaggc atgaaatatt tatcgggatt ttctttgaat
      901 aattttcttg ctagcattat agcgccgtca gagctttcga gcgggtttga gtaaattata
      961 tcagccccaa aagcctttaa tattttcttt ctttcttcgt ttacattttc aggtattaca
     1021 agctctactt tgtatccaag agctgcacct atcatcgcat aggcaatggc ggtgtttcct
     1081 gaagatgagt ccattatgat cttgtctttt gtgagttttc ccttctctat gccgtctaca
     1141 atcatcttaa gcgcaggcct gtcctttaca gagcctccgg ggttaaacca ctcagccttt
     1201 atatacagct ccactcctgg cgcaagctgc ttttcgtaat ttgtaagtct tataagcgga
     1261 gtgttgccga taagctctat tacattgtga actgcgttct tgttttcggg gggatattga
     1321 atcgcctgat ctgatgtgct cattaatgct tttctatgat tactttataa aaatcctgtt
     1381 ctgatttatc tatgcttacc actttgtgcc cgtcttcttc tgcgcttctt ggtacattca
     1441 taagaggctc gccttctcta agtgtaacct ccaaaacctg ccccgcaccc attgtttcca
     1501 attttagttt ggtctttaca aatgtcatcg ggcaaatctc gtttgttatg tctattcttt
     1561 catcaatcat aatatcatcc ttaccactca tcttatttag tataagatta atattaaaca
     1621 aaacaactta ccatattggt tttttaagac aatgtgttga aaatgatctt tatggataaa
     1681 tgatataata tagtagggat attgttatgg tcgaatattt aactgtatat gtagaattac
     1741 tcaatgaagg tgtaccctgt aggaggccgg ttcctgcatg tgaggtggag ccaggtgtct
     1801 acatacttca caagacttat agttatcatc cggatgatga ggagtgggag tttttaccgg
     1861 gtgataaagt gagctgcgaa gaacgtatta gcaacgatgg taaaaaaatt cttgttgcta
     1921 taactctagt agagaaaaga aaaagagagt ggtgaaatat cttctcaagg ggtgcgaaga
     1981 ttcttcgcag gtacccctga agattcttcg cactaaggac tacacatata aggaatacac
     2041 agtaaggaat gggcaatata tttaaaactg gggcaaagat aaatctaggg ataatttcta
     2101 ttcctattct tgctatggtt gcatcattta taggcttgaa aatgtgggaa gcaagctgtg
     2161 agtctgagtt ttggaaacag tactattcac ctgataagga gtggaaagta gttcttacta
     2221 aaactgattg ttcaccattc aatgagctta gttatgaagg tgatatttgc agagtttatt
     2281 atgtgcaaaa ttgttctaat aaaaatattt tttcatttta tgcgactgat caattagaca
     2341 accttagaaa tgcaagtgat ttattagaaa tagtctggaa tggtaatagt agcgtttcag
     2401 tgatgtatga tagcagactg tatttcgatg acaggcattc agttcataaa ggaattaaga
     2461 ttacatatca taagaaagag gagtgattgt gttttgttca tagaaataag tagctggact
     2521 tcggcagcat ttacctgaca cccaggcaaa ttttacccgg gtacccaggc aaaatttgcc
     2581 tactaaggac tacacatata aggaatacat agtaaggaat aatatttata ttataataat
     2641 ttttggagct cgcatagaac agagctataa gtaatgcaga atctccttta ttttattaac
     2701 taaatctaca tttctgttat taaatctaaa ttccatctct tttagataca aaacaaagtg
     2761 tt
//
LOCUS       DBNGSV010000158         5352 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1828282, whole genome shotgun sequence.
ACCESSION   DBNGSV010000158 DBNGSV010000000
VERSION     DBNGSV010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1828282"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..760)
                     /locus_tag="AC159U_05520"
     CDS             complement(185..760)
                     /locus_tag="AC159U_05520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3336549.1"
                     /translation="MSNQATQTAENMLNGVSVDGIFSTIEAVQENGEIAKFKFRNSNN
                     WVEGGLNRSEISGFYGACQEHGDRNFTLENDEPPVLLSKDQAPNPVEYVLHALAGCIT
                     TTLVYHAAAKGIEIQEMETKFEGDLDLRGFLGLPGAKRRGYEEIRISMRVKADATDEQ
                     IEELVDLAQRRSPVFDTVTNPVPVKINLIKD"
     gene            complement(952..2886)
                     /locus_tag="AC159U_05525"
     CDS             complement(952..2886)
                     /locus_tag="AC159U_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HIR3336550.1"
                     /translation="MTTPDKSPYDELDIDETLKTILEGTSSKVGKEFFKVLVFNLAKA
                     LDTYAAWITELESAQKASSIAFIMNGKWVKEYVYDLKNTPCEPVIMGRELVHIPDNVV
                     KLYPKDPDLITFDPIPLSYMGIPLIDSNGSVLGHLAVMDSKPMPENPRNVAIMNIFAA
                     RALAEMQRLKAEAKVLEREKKHMRLLSSAMDAIAEFDEDLKITQVNRSAEKILGSCSE
                     VLVGTNYATMMSKASLEKLIKIIDKLEDKKEGEKNLWIPAGLELTDVDGNKFQAEATI
                     SQLDINSKKYYTLIFRNVTHRIEAEKKIESLSSQAEYLKEEIKSQHNFEEIIGKSKSL
                     QDVINNVKKVSTTNSTVLIYGETGTGKELIARLIHAESKRSDKPLIKVNCAAIPAALI
                     ESEFFGHEKGAFTGATSKREGRFLIADGGTIFLDEIGELPLDLQSKLLRVLQEGEFEP
                     VGTSKTIRVDVRIIAATNRDLLKEVESGNFREDLYYRLNVFPITVPPLRERGDDVITL
                     AEAFCKKYAGNIGVKLNPIDKGNIEFLKNYRWPGNIRELQNVIERAVITSQDGSINLI
                     GELPAGSDSLAKNNIESIDDDRVYTVKELSNIERDNILRALEKTGWKISGQSGAANLL
                     GIPPTTLNSRIKALNIKKPS"
     gene            complement(2942..3526)
                     /locus_tag="AC159U_05530"
     CDS             complement(2942..3526)
                     /locus_tag="AC159U_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3336551.1"
                     /translation="MFNRTIKTIAVIMLIIASAGSLYAKEVWQWTDKEGTTHITDNKA
                     KIPEQYRETAKNLNWNDSSNNENVFKDTLDADDTVIITIEEEPYVDGIIDTEHEAELR
                     EEWRAKMVAIEQEQAEVESKLAQATEDQKYKKREVDWYLRNGYPADYMIAELKGIERY
                     IEDLEEELTTIPPKIDALQIEARKAGVPPGYLRE"
     gene            3664..5259
                     /locus_tag="AC159U_05535"
     CDS             3664..5259
                     /locus_tag="AC159U_05535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR3336552.1"
                     /translation="MPKLTIDGIEVEVEDGLNLIQAAEQVGIEVPHFCYHPDLSIVAQ
                     CRQCLVEVEGVPKVLPGCNTFVRDGMEVKTNTEKAIEARKATVEFTLINHPLDCPICD
                     KGGECPLQLTTVKHGPGYSRFDTPYEKKVRQKYYLSDRIMYDPNRCIMCTRCVRFTDE
                     VTKTGELGYDNRGFRKKIVTFPGKPLDNELAGNVIDLCPVGALLNKGTLHEERVWYWK
                     FTESVCTLCSTGCNITVGVDPRKGKVSRVRPRFNKDVNGHWICDRGRYGFEDVQEEKR
                     VKEPIIKVKNGFDITDWSNAYELIEENFPVSGGFADNNIGIIGSPRLTNEELFLLTKL
                     KDSVRASGLVSNIGSATGEKKFDLISSDPYPNSKGVTALGIDHTPGALDDLIDGILDE
                     KVRKLIIVENDLFDIIHESQHYILEGALNSLDYLLVIDTKLTETMKYADLILPAATAY
                     ERDGTFTNDELRVQRLNKSIDPPGTAKPQWEIINDLIEIIGDTKSAFDSPGGVFNTIS
                     ENIPTYNNMTYDKLGFLGLAKQV"
BASE COUNT         1410 a         1150 c         1123 g         1669 t
ORIGIN      
        1 taatcgcggt tgttaatatt actccgagag gggatgctta taaataatat aatctctttt
       61 tcactccctc aaatactgtt agggcacttg ctctgccgag gtcttgatgg tttgaaaaat
      121 tagggacgga acagatagta aaagggctgc cgaatagcaa ccctttacta tgaatggtca
      181 tgttttaatc ctttatgaga tttattttta caggtacggg gttagtaact gtatcgaaaa
      241 cgggggagcg tctttgggca agatcaacca gctcttcaat ctgctcatct gtggcatcgg
      301 ctttaaccct catcgaaatc ctgatttcct cgtagcctct tctttttgca ccgggaagtc
      361 cgagaaaacc tctcagatca agatcgccct caaactttgt ttccatttcc tgaatttcta
      421 ttccctttgc tgcggcatga taaacaagcg tagtcgtaat gcagcctgca agtgcatgaa
      481 gtacatattc gacaggattt ggagcctgat ccttactaag tagaaccggc ggctcgtcat
      541 tttccagagt gaagtttctg tcaccatgct cctggcatgc gccatagaaa cctgaaatct
      601 cagagcggtt tagccctccc tctacccagt tgttagagtt gcggaactta aacttagcta
      661 tttctccatt ttcctgtacg gcctctatgg ttgaaaagat accgtctacg cttactccgt
      721 ttaacatatt ctctgctgtt tgtgttgctt ggtttgacat ttgtcttacc tcctaaattt
      781 gaatttgatt tatataatta caataggcgt gccaagcctc attccagaga aataagacaa
      841 tgagtacagt atgttatgat gataatcgga atatttcagg actaacgaga attcgtgatt
      901 aacgagatat cgccactaat cttggcgaga tgtcgtaaat taatataaag gttaggaggg
      961 ttttttgata ttaagagcct tgattcttga attaagcgta gtgggaggga ttccaagcag
     1021 gtttgcggca ccgctctgtc cggaaatttt ccagccggtt ttttcaagcg ccctcaggat
     1081 gttatctctt tcaatgttag aaagctcttt tacggtataa accctatcat cgtctatgct
     1141 ttcaatatta tttttagcaa gggaatcaga acctgccgga agttcaccga tgagattaat
     1201 actgccatct tgagatgtta ttacggctct ttctatcaca ttctgaagct cccttatgtt
     1261 gccgggccat ctgtaatttt ttagaaattc gatatttcct ttatctatcg ggtttaattt
     1321 gaccccaata ttacccgcat attttttaca aaacgcctca gcaagggtaa tcacatcatc
     1381 acctctttct ctcagaggcg gtacggtgat aggaaagaca ttgagcctgt aatataaatc
     1441 ctccctgaaa tttcctgatt ccacttcttt taacagatcc ctgtttgtgg cggcaataat
     1501 cctcacgtcc actctgattg tctttgaagt tcccacgggc tcaaactccc cttcctgcag
     1561 cactcttaga agtttggatt gaagatcaag cggaagctcg ccgatttcat ccagaaatat
     1621 cgtacccccg tcggctatta aaaaccttcc ttctcttttt gatgttgccc cggtaaaagc
     1681 gcctttttca tgtccgaaaa attcactctc gataagggct gccggaattg cggcgcagtt
     1741 tacctttata agcggtttat cactcctttt gctctcagca tgaatgaggc gcgcaatcag
     1801 ttcctttccc gtgccagtct ctccgtatat aagcactgta gagtttgtgg tagaaacttt
     1861 tttaacatta ttaataacat cctgcagtga cttgctctta ccgatgattt cttcaaagtt
     1921 gtgctgggat ttgatctctt cctttaggta ttccgcctgg ctggatagtg actctatctt
     1981 tttttctgcc tcaatcctgt gggttacatt tctaaaaata agtgtgtagt atttcttgct
     2041 gttaatatcc agctgtgaaa ttgtggcttc tgcctgaaat ttattaccgt caacatcggt
     2101 gagttcaagc cctgccggta tccacaggtt tttttcacct tcttttttgt cttcaagctt
     2161 atcaataatc tttatgagtt tttcaaggct tgcctttgac atcatagttg catagtttgt
     2221 tccaacgagc acttcggagc atgagccaag gatcttttcg gcagacctgt ttacctgagt
     2281 gatttttaaa tcttcatcaa actcggcaat tgcgtccata gcgcttgaaa gcagtcgcat
     2341 atgttttttt tctctttcta agactttagc ttctgctttt agtctttgca tttctgcaag
     2401 cgcccttgcg gcaaagatat tcattattgc cacgtttcta ggattttcag gcatgggctt
     2461 tgagtccatc acggccaggt gtccaagcac actgccattt gaatcaatta gaggtatgcc
     2521 catataacta agcggaatag ggtcgaaagt gataagatcg gggtctttgg gataaagctt
     2581 aacgacgtta tcaggaatat gtacaagttc ccttcccatt attacgggtt cacagggcgt
     2641 gtttttaagg tcataaacat attccttaac ccacttgccg ttcataataa atgcaattga
     2701 gcttgctttt tgggctgact caagttccgt tatccatgct gcgtatgtat caagagcctt
     2761 tgccagattg aaaactaaaa ctttaaaaaa ctccttgcct actttgctgg atgttccctc
     2821 gagtatggtt ttcagtgttt catcaatgtc tagttcgtca tatggtgatt tatctggagt
     2881 agtcatgtga tggattaaaa cttattgtaa ttaaatactt aatttataat actactataa
     2941 atcactccct caaatacccc ggtggcacgc ctgctttgcg ggcttcgatc tgcagggcgt
     3001 ctattttggg ggggatggtg gtgagttctt cctcaaggtc ttctatatac ctttcgatcc
     3061 cttttaactc tgctatcata tagtcagccg ggtagccgtt tctcaaatac cagtccacct
     3121 ctcttttttt gtacttttga tcctctgtgg cctgggcaag cttgctctca acctctgcct
     3181 gctcctgctc aattgctacc atctttgccc tccactcctc tcttagctct gcttcatgct
     3241 ctgtatcaat aatcccgtcc acatagggtt cttcttcgat agttatgata actgtatcat
     3301 cggcatcaag ggtgtctttg aatacattct cgttgttaga cgaatcattc cagtttagat
     3361 ttttggctgt ttccctgtac tgttcgggaa tcttggcctt attatccgtt atatgcgtag
     3421 taccctcttt gtcagtccac tgccagacct cttttgcata aaggcttcct gcagaggcaa
     3481 ttataagcat aattactgct attgttttta ttgttctgtt aaacacaaat atccgcctgg
     3541 ttatataaca aaatattgat gcctaatagt ataatctaat aattctattt ttgctcaaaa
     3601 gtcttgcttc tttatgaatt cttggtaact taacaggcgt tatttcactc ctatttttat
     3661 aagatgccaa agttaactat agacggaatt gaagtagagg ttgaggatgg tttgaacctc
     3721 attcaggctg ccgagcaggt tggcatcgaa gtaccgcatt tttgctatca tcccgaccta
     3781 agcatagtag cacagtgcag gcagtgcctt gtagaagtgg aaggagtgcc caaggttctt
     3841 ccaggctgta acacctttgt acgtgacggc atggaggtta aaaccaacac cgaaaaagcc
     3901 attgaagcaa gaaaggccac agtagaattt actcttataa accacccgct tgactgccct
     3961 atttgtgata agggcgggga gtgtcctctt cagcttacta ccgtaaagca cggcccggga
     4021 tacagcaggt ttgatacccc ttatgaaaag aaagtaagac agaaatacta cctttcagac
     4081 cgcataatgt acgatcccaa ccgctgcatc atgtgtacaa gatgcgtgag gtttacagat
     4141 gaggtgacca aaacagggga gcttggatac gataaccgcg ggtttaggaa gaaaatcgta
     4201 acctttcccg gaaagccgct tgataatgag cttgcaggaa atgtgatcga tctctgcccc
     4261 gtaggtgcgc ttcttaataa aggtactctt catgaagaga gggtatggta ctggaagttt
     4321 accgaatcgg tatgtacgct ttgcagcacg gggtgcaaca tcacagtggg ggttgatccg
     4381 agaaagggaa aggtatcgag agtacgtccg aggtttaata aagacgtaaa cggtcactgg
     4441 atttgtgacc ggggaagata cggcttcgaa gacgtgcagg aagaaaagag ggttaaagag
     4501 ccaattataa aggttaagaa cggattcgat attactgact ggagcaatgc atatgaactt
     4561 attgaagaaa actttccggt ttcgggcgga tttgctgata acaacattgg aataataggc
     4621 tcacccaggc ttacaaacga ggagctgttc cttcttacca agcttaagga ttcggtcagg
     4681 gcaagcgggc ttgtttcaaa tatcggaagc gcaacaggag aaaagaagtt tgaccttata
     4741 agctccgatc cctatccgaa cagcaaaggt gtaacagcac tcggcataga ccacacgccg
     4801 ggagctttag acgacctgat tgacggcatt cttgatgaaa aagttagaaa attaattatc
     4861 gtggaaaatg acctgttcga tattatccat gagtctcagc actatatact ggaaggcgcg
     4921 cttaacagcc ttgattacct cctggtaata gacactaaac tcacagagac catgaagtat
     4981 gcagacctca ttctgccagc ggcaacggcg tacgagagag atggcacttt tacaaatgat
     5041 gaattaaggg tgcagagact aaataaatcc atcgatcctc ctggcacggc aaagcctcag
     5101 tgggaaataa taaatgactt gattgaaata ataggcgata caaaatcggc ttttgatagc
     5161 ccgggcggag tttttaatac aatttcagaa aacattccga cttataataa tatgacttat
     5221 gataagcttg gctttctggg cttagcaaag caggtttaat aaaaaatgga attagtagat
     5281 cttggattta cgcttcttaa aatcgggctt gtactattta tagtgctcac gcttgttgcg
     5341 taccttacac tt
//
LOCUS       DBNGSV010000159        19880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1759830, whole genome shotgun sequence.
ACCESSION   DBNGSV010000159 DBNGSV010000000
VERSION     DBNGSV010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19880
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1759830"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="AC159U_05540"
     CDS             complement(<1..600)
                     /locus_tag="AC159U_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR3336553.1"
                     /translation="MEESSFSELLNESLDIPSAGKKVTGTVIRVESDEVFIDFGFKSE
                     GVVPREEFDVKNGDSEVQIGDQVVVILENFNSQSGLPKLSKKKAEIQDEFQRINDINA
                     GNEKVTAKILQDVKGGFIVDIGQKTEIRAFLPGSQVDIRPVRDYKKLIGQEIEARIIN
                     MTDKGIVVSRRVLLEELRENRINELMSTLNVGDTITGKIS"
     gene            complement(689..1360)
                     /gene="cmk"
                     /locus_tag="AC159U_05545"
     CDS             complement(689..1360)
                     /gene="cmk"
                     /locus_tag="AC159U_05545"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3336554.1"
                     /translation="MEKRVVIAIDGPSSAGKGTVAKIVADKLGFAYMDTGAMYRAVAL
                     TLRINKTDIEDENEISDVLNNTHIYLYNDNNGNLVVELNGKNVSNEIRTPEISRFSSD
                     IATKKSVRDFLLQLQRNFGNNKSIVAEGRDMGTHVFPDAEFKFYIDASVQERTKRRFA
                     QLKEAGITVDIEKLKKDIENRDYQDKNRQIAPLHPATNAVIIDTTNLQIDQVVDIILK
                     KTTGV"
     gene            complement(1362..2648)
                     /gene="aroA"
                     /locus_tag="AC159U_05550"
     CDS             complement(1362..2648)
                     /gene="aroA"
                     /locus_tag="AC159U_05550"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3336555.1"
                     /translation="MAREQIKGGPTSIKGEITLPGDKSISHRAVIFGSLADGVTNVEG
                     FLKSEDTFSTIKAFEAMGVDISWQGEILRINGAGLHGLKEPEDMINAGNSGTTSRLLI
                     GLLSGQNFSSSITGDKYLKKRPMGRVINPLREMGANIDATDDSKLPLTIHGTNLNGIS
                     YKMPVASAQVKSAILLAGLYAEGQTKVIELSPTRDHTEKMMQYLGVDISTDSNSIRIS
                     KSCGFKARDITVPSDFSTAAFFIVAALINPGSEILIKNVGVNPYRIGALEILLSMGAD
                     IELTNERELNGEQVADIVARSSFLKGIDIGGDMIPKAIDELPIIAVAACFAEGRTTIS
                     DAEELRVKETDRITTTVSELKKLGAKIEETEDGMIIEGTDGLVGANCSSCGDHRIAMS
                     VAIAATRAKGQTIIEDSGAAAVSFPEFFEILNNIKA"
     gene            complement(3033..3287)
                     /locus_tag="AC159U_05555"
     CDS             complement(3033..3287)
                     /locus_tag="AC159U_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336556.1"
                     /translation="MFNVDDFVNWAYGNLLNNTTRAVVGEYLVHKALDSSTKQRIDWD
                     AYDILYKDYKIEVKTSGYLQTWNQQGNYSKIKFDISKKDP"
     gene            complement(3357..3800)
                     /locus_tag="AC159U_05560"
     CDS             complement(3357..3800)
                     /locus_tag="AC159U_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336557.1"
                     /translation="MKYLLLLIISFSLFAIPQKSNAEDVPERYKVVIEESYYVIAHEN
                     KEEFMKIYKERIFPFWREMKKMGLIEDDIRMYSQRIHPKKPHWTHKTVVRFKNYHAID
                     KWLASREEVVDKLFPDEGSYKEMRGKIAAITEAHWDDLVRELPLE"
     gene            complement(3897..4385)
                     /locus_tag="AC159U_05565"
     CDS             complement(3897..4385)
                     /locus_tag="AC159U_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336558.1"
                     /translation="MMRIITISLKLSIFFLLANLYLTYTAVSVEQINHTVGFGTLKWG
                     TELDSLDNLKFVKSGKTSQGEIKIYKNTNDNLDYNGVELKDIEYGFNNDKLYFVAFKT
                     NGPENWKELKEHALQKFGNEFNKIEDFGYQNYVWQRQESSVSLEYKPKQNSSVVLLIV
                     SN"
     gene            4768..7221
                     /locus_tag="AC159U_05570"
     CDS             4768..7221
                     /locus_tag="AC159U_05570"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR3336559.1"
                     /translation="MASEKIDESHSNGNGTAAVEKQKSISADIEASTEAKNDGGYTEG
                     LVDIPEETIEAFGGDELRARVFFEKYALRDKSGKMVEKSPADMWRRVAKEIASPEKTT
                     DLKKEWEENFFWLLTDFRFIPGGRILFGAGQKRRATLLNCYYMPIKEDSIEGIFEWCK
                     EAARTYSFGGGVGTDISILRPKGAPVNNSAIYSTGAVSFMELLSTTTGTIGQAGRRGA
                     LMITLGVNHPDIEEFIDIKNDADRAKVNFANISIKITDDFMRAVENDTEFELSFESEK
                     VKVTKKVHARKIWNKLIKSAWASAEPGVIFWDAVKRYSPTEYNGMEVNGVNPCSEQAL
                     EDYGNCCLGNINLSLFVKNSFTEDASIDWENLEETCKYSVRFLDNILDYNMQKHPLKY
                     QTNASTLSRRIGVGFTGYGDMLAKLNIKYDTDEAVEFADKMFEHIKNTVYEASSDLAA
                     QKGSFPAYDEEIHLNQPFLKAIDKNVYQKIKKQGVRNACILTVPPVGSGSVLAGTSSG
                     VEPIFALSYYRRSKSLSKEEFKVYHPLVGEYLEKFGLEDESEVPDVFVTSHEIKPEMR
                     VRTQAAIQKHIDSCISSTVNLPTDISLKEVEQIYFLAWKLGCKGITVYREGSREGILV
                     TNEEGVKQQQAKEEAEHFQPRDEGQKTPQHPISTPLHPENEIKPVKRPQTLEGFTEVI
                     KTGYGSLYVTINTYEGNPFEVFIQIGKSGYSTMADAEAIGRLVSLSLRSGVNVKDVVE
                     QLEGIGGASQVYSEGKLVMSIPDAIATILRKHFMDEKKVSKKTSDIDLEVCPDCSSRS
                     LAFESGCVTCRSCGYSKCD"
     gene            7580..8023
                     /gene="mscL"
                     /locus_tag="AC159U_05575"
     CDS             7580..8023
                     /gene="mscL"
                     /locus_tag="AC159U_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3336560.1"
                     /translation="MLDEFKKFAMRGNVVDMAVGIVIGGAFALIVNSLVNDVIMPPVG
                     MLLGNVDFSNLHAVLKEGTTAGPYISLAAAKEAGAVTLNYGAFINTIVSFIIVAFAIF
                     MVVKGMNKMQEEEEAPEEPTTKECPHCISTISIKATKCPNCTSQV"
     gene            8089..8874
                     /locus_tag="AC159U_05580"
     CDS             8089..8874
                     /locus_tag="AC159U_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR3336561.1"
                     /translation="MKIAIYQIDAFTSELFRGNPAAVCPLDKWIDDSLMQSIAAENNL
                     SETAFFVREDDGYRIRWFTPTTEVDLCGHATLAAAYVIFNEIDKSVEKIKFYSNSGEL
                     NVTRDSDLITLNFPSLPPNISKKPEKVVSAFNIEPMEVFEADDYLLIYENQEQIENIN
                     PDLNLLREIDLRGVIVSTPGNDCDFVSRFFAPKYGIDEDPVTGSAHCTLTPYWSEKVK
                     KKKLHAKQLSKRGGELFCELKLYSVSISGKAVLYLIGEIEIHL"
     gene            8941..9885
                     /locus_tag="AC159U_05585"
     CDS             8941..9885
                     /locus_tag="AC159U_05585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3336562.1"
                     /translation="MKTLIFGAGPIGSLYAHLICQAGKDVTILARGKRYEFIKTKGIE
                     LVNEITGDRGASKLRVVNELKEDDEYDLVVVLIRKNKLLPVFEVLRTNANIKNILFMG
                     NNSLGFVDYLRYLPKEKLLLGFPGAGGGISEQVVHYADREKPKDKRRPVTIGEIDGKT
                     KRRTLEIKSLFEMSGIPVDLVGDIDGWLKCHVALISPLGNALYKHNCENYSLAKDKKT
                     IKVMIQAVKEGGRVLKALGYTKRYPFKFNLFYWLPEMLNVKAVEGLLESKFAEVAFSL
                     HARAAQDEMRELSVEFQTLINKTSVETPNIVTLRNFIL"
     gene            10088..10456
                     /locus_tag="AC159U_05590"
     CDS             10088..10456
                     /locus_tag="AC159U_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3336563.1"
                     /translation="MNITGIMIFCEDIKNMTAFYRDVVGLTPLDTQPFPAHKFFKFVS
                     KKGAELCLHSGTKPNGGRQKLMLYTDDIDGLVKRVKAYKPRVRLPEPDKDGNIVFDFR
                     DPEQNRIQVYSNAIYRNRYT"
     gene            10486..11202
                     /locus_tag="AC159U_05595"
     CDS             10486..11202
                     /locus_tag="AC159U_05595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008988724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3336564.1"
                     /translation="MPKKYLFFLLVLFPAAIFLLYYYGRSVWVPKYRALAGQRTVADV
                     ISIYGEQAVARLAPYFKNAGVIYPPKQVVMLATKDDKELELWASDGADFKYIRTYDIK
                     KTSGKLGPKLREGDHQVPEGIYRIIGLNPNSSYHLSLKLNYPNDFDLLQAKKDGRTNP
                     GSDIFIHGKALSVGCLAMGDNAIEELFVLVENVGKQNVKVVIAPFDPRKQDLVVPEGS
                     NPIWLGELYNQIEEEFIKFN"
     gene            complement(11219..11386)
                     /locus_tag="AC159U_05600"
     CDS             complement(11219..11386)
                     /locus_tag="AC159U_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336565.1"
                     /translation="MSTAYRKIGDCTNALKSADKALEVMDFKAAQYAKSTAELCIEMQ
                     KMVYKDIVKSK"
     gene            complement(11393..12103)
                     /locus_tag="AC159U_05605"
     CDS             complement(11393..12103)
                     /locus_tag="AC159U_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336566.1"
                     /translation="MIKRFILNTTVFLLFSNIAICDEIDQLLQKALDAKFSEVGNDSS
                     ARQRYLFEILQKEPDHLEALWQVTLIKLNSIPSSSLSKEAPYLAMLGPEVNKILDTAI
                     SKGNRAFYHYINAVYASAHKAYDTALIEIEKSINLEPQSARYVLNKGKILIDKGRWTY
                     NDDNIAQGLDMIEAALILSKQNKNPFHSPWHYNFQLALGNSYFENPKWQEVISNYQIS
                     IDIMEKVNATQKSSYAYA"
     gene            complement(12100..12795)
                     /locus_tag="AC159U_05610"
     CDS             complement(12100..12795)
                     /locus_tag="AC159U_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3336567.1"
                     /translation="MISDRKLFYIPCPFFKVYKEFCNLNKKSLDSGIACVDPEVRGAR
                     LPFQAETGDYLKNLYNSKLNLIAGKKCSISGLSDEISSVKIPSFLHIGAHGSFYSGKP
                     MESSIYLSVSENSQSGIWTAHDIATTDLGNFPLVTLSSCETGLSDIDTERDVFGFHRA
                     LFFAGAQSILSPLWAVHDKGTAFLMQNFYKSYSSGDNPATSLKKAQLLSIKNKEYNHP
                     YFWSGYTITGLLL"
     gene            complement(12723..14234)
                     /locus_tag="AC159U_05615"
     CDS             complement(12723..14234)
                     /locus_tag="AC159U_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3336568.1"
                     /translation="MDLDSKKGRKQLSRGNYKGAYKTYQEVLAISYNNKNLRGIGVSL
                     GYIANILNKAGDKENALKTYLLQHKITETMHAPHDLGVVQLSISRMLAYKNESLSLMW
                     LMKARDNLKGTHYKRDTVSVLEELGSKHYYQGHKEKAGIFYKDAWDLALTLGKSRYDR
                     WARWEAGKLYSIYLQKTDNYQNSLNILEHITKEFPVVEMQGEMYSETLFLISKSYSKL
                     KYTEQADNSYHKAYIAYQHARSKSLGDVGRAKLDSKFNNYIDEYINHLITNNKYMHAL
                     VTIESNKAPTLNDIMEDVYQKDVYQKLSDLNTKHQLEIQDFIKQNSQTSEISDENILI
                     KYSQITQKQQQEKKELEISLNIRTVPVSSSLSANDIHLAQNHLPDDTAILSFYISKEL
                     SGLFILLNNSKKFVKLNIDENSLYLLASELRASIINPYTDYYKESSVKLYSALFGEVL
                     KHLNKNIKNLIVVSDNVLSSIPFSPLYDGKSFLIENYSIYRVPSLRFIKSFAI"
     gene            14605..15210
                     /locus_tag="AC159U_05620"
     CDS             14605..15210
                     /locus_tag="AC159U_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR3336569.1"
                     /translation="MNKYIEYVLDFWFGDIRDGLIVENRNDFWFMPNSQIDNTIREEF
                     EELVVKAGKGKLIHWEQSAKGTLALIILLDQFTRNIYRGKREAFQFDAEALRICKAGI
                     QRGHDRELEVIEKCFYYLPLEHSENLEEQDKCVKLFEDLLVTVDSNKVSIVKNSLDYA
                     VLHRNIIEKFGRFPHRNEVLNRKSTNEELVFLSKQGNKFGQ"
     gene            15258..15722
                     /locus_tag="AC159U_05625"
     CDS             15258..15722
                     /locus_tag="AC159U_05625"
                     /inference="COORDINATES: protein motif:HMM:NF021361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HIR3336570.1"
                     /translation="MNNKIILAAFSLFLTFTAFSIADETSHKKAAQELLVTMDIQNML
                     EKSNKRMVAIQVARNKRISGYEEVLNNFYNKYSNWDIVKDDVIELYTIKFTEAELNEL
                     ARFYKSPVGQKALAEVPTLMLETIAAGQKNIEKNLPELQQQIQKLQEEKKKN"
     gene            15774..16328
                     /locus_tag="AC159U_05630"
     CDS             15774..16328
                     /locus_tag="AC159U_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336571.1"
                     /translation="MNSISNHLIILALFACSFYISPHSYSLTIEEAYSAIPHRRTEFD
                     YNSAKMSLEEKTYLSEVFELTDLATIEKVNMLMWLGSNGQKGEVAKHYSNIISSLYNL
                     NPPSGLDNFHNLIISSIKEQKETLFKWKISSFDYSMIRSESLVRSSSKKLKSAYRILK
                     KKYPGESSYNKRAFFDHLCALDFI"
     gene            16372..17352
                     /locus_tag="AC159U_05635"
     CDS             16372..17352
                     /locus_tag="AC159U_05635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3336572.1"
                     /translation="MPSIKDTSNQMIQFVFPEHANNYGTLHGGRLMNWIMVLGSITSS
                     RIAKGLIVLGTADSIDFLNPVRTGEVVLLNSWVEYIGTSSMEIGVKVESENTSTGEKK
                     LTTFSHLAYVAIDENGSPKKVENKISPADDDERKVYKDAKKRKAQRIERLKDTKPELK
                     DIKYLKNYEWQIETIRVVLPEDAFYGNYLSVGKLMMDIDETGAILARKFTRGVVVTGS
                     VDDVHFYEPIRVGELITLHARINYIGRSSMDIEVNVHTENLTTAVKKHSCTAYLNFVH
                     ISKEGRPKAIDKIITPSTKYQKKLWKQSEDRKDRRFERVKWIKNNMVNDN"
     gene            complement(17380..19119)
                     /locus_tag="AC159U_05640"
     CDS             complement(17380..19119)
                     /locus_tag="AC159U_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336573.1"
                     /translation="MDENKQINKALFPELNEFAKISTWYPNAVTQVSETLTTTSSMFS
                     GHDELKNCSGSEELCGHTVIWNLVKGKIPTKDIRNLNGIKQKTNKIDLWTSIDKYKVQ
                     KDTLRILIEPGLNNSLKNFLKQIIDLYLLSIQNSSWSVKIFGADTYLDLTSSNHAKGA
                     IVDGIPKSYGFLACATKANRAIIRFNLFTDRIDPQRYFINSILTSLTHTPLCFDSDGI
                     IEVVYPIRPLLDDNEKVNYLPDAVAMSEYAQTSCTSDEISINIRRARINQSMLAMKMF
                     NKTITRIKELGLFDKSLIIAVSDHGVGFLGPSFGRANHKLRNSEDILDNRMMNASTLL
                     MIKYPHQKQPQTDYRMVRPIDIGATLMDIIEFDPPWDIDGQSLLSDNWKNLSPPLDFF
                     QIMYEDNQWKNKTDSGTFLKGKISEPLKYFDTIKHKFNKLTVYKSKWIDKNAEQLPLM
                     RQKSGKLEYLKIEKTLPTENSQDYILTLGGYSVDPKLNPASLTYISVNGKIIKAIKPC
                     LSFNKSYHYIGSILAGWVVSIPSEFIKSKNIKIRAFSKVENSNEEFFYELENPINIEL
                     TDEQFNKVGYYDN"
     gene            19469..19651
                     /locus_tag="AC159U_05645"
     CDS             19469..19651
                     /locus_tag="AC159U_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336574.1"
                     /translation="MDTLLFIKLNMVNNRVNKIVGMINISVGRVSELTRGFKNGGGNS
                     LKSDENTWNIVIPIPT"
BASE COUNT         6349 a         3357 c         3671 g         6503 t
ORIGIN      
        1 gctgattttt ccagtaatgg tgtcaccaac atttagagta gacataagct catttattct
       61 gttttctctc agctcttcca aaagcactct gcgggaaaca acaattccct tatctgtcat
      121 attgatgatt cttgcttcaa tctcctgacc tattagtttc ttatagtctc tgactggtcg
      181 gatatcaacc tgtgaaccgg ggaggaatgc gcgaatctcg gttttttgtc caatatctac
      241 aatgaaaccg cctttaacat cctgaaggat ttttgcagtt actttttcat ttccagcatt
      301 aatatcgttt attctctgaa attcatcctg gatttcagct tttttctttg atagtttggg
      361 aagtccactc tgcgaattaa aattttcgag aataacaacg acctgatcac caatttgtac
      421 ttcggaatca ccatttttaa catcaaattc ttctctgggt actactcctt cagacttaaa
      481 cccaaaatca ataaagactt catcactttc tactcttatc accgttcctg taactttctt
      541 gccggcactg gggatatcca atgattcatt taatagttca ctaaaacttg attcttccat
      601 aatttataaa atcccgcctg attgtttatt ttgatcgggg agaaattcca tatatatcta
      661 aaaaattact atttcaatcc ttgtgtttct aaacccctgt agtctttttt aatattatat
      721 ctactacctg atcaatttgt aaatttgtag tatctattat tactgcatta gtagcgggat
      781 gcaagggagc gatttgtcta tttttatcct gataatctct gttttctata tcttttttta
      841 atttttcaat atccacagtg attccagcct cctttagttg agcgaatctt ctttttgttc
      901 gctcctgtac agaagcatct atataaaatt taaattcggc atccgggaat acatgagtgc
      961 ccatatccct tccctcggct acaatacttt tgttattacc gaaatttctt tgtagttgca
     1021 ataaaaagtc tctaactgat ttctttgttg ctatatcaga tgaaaatctc gaaatttcag
     1081 gggttcttat ttcattactt acatttttac catttaattc tacaactaga ttgccgttat
     1141 tatcattata taggtaaata tgtgtattat ttaaaacatc tgatatttca ttttcatctt
     1201 caatgtctgt cttattaatt ctgagcgtaa gagcaacagc cctatacatt gcacctgtgt
     1261 ccatgtatgc aaatcccagt ttatcggcca ctatttttgc tacggttccc ttacctgccg
     1321 atgaagggcc gtctattgcg atgacaactc ttttttccat tttatgcctt gatattattt
     1381 aatatttcaa aaaattccgg aaatgacact gctgctgcac ctgagtcctc gattatagtc
     1441 tgaccttttg ccctagtagc tgcaattgct acagacatcg cgattctgtg atcgccacaa
     1501 ctactgcaat tagcacctac taacccatca gtaccttcta taatcatgcc gtcttcagtt
     1561 tcttctatct ttgcaccaag cttcttaagt tcggataccg tagttgtaat tcggtcagtt
     1621 tctttgactc taagctcctc cgcatctgat atagttgtcc taccttcagc aaaacaagcg
     1681 gccacagcaa ttataggcag ctcatcaatt gcctttggaa tcatgtcgcc gcctatatct
     1741 attcctttaa gaaagcttga tctggctaca atgtcagcaa cctgctctcc gtttaactct
     1801 ctttcgtttg tgagttcaat gtctgctccc attgatagaa gaatttccag agcacctatt
     1861 ctgtatgggt ttacaccaac gtttttaatc agtatctcag agccagggtt tattagagct
     1921 gcaacgatga aaaaagctgc ggttgaaaaa tcggaaggta cagtaatatc ccttgccttg
     1981 aatccgcatg atttggaaat cctgattgag ttagaatctg tggaaatatc tacacccaaa
     2041 tactgcatca ttttctctgt atgatctctt gttggagaaa gctcaatgac cttggtttgg
     2101 ccttcagcat agagtccagc tagtagtatg gccgatttta cctgtgcgct tgctacgggc
     2161 attttgtagc tgattccatt cagatttgtt ccatgtattg taagcgggag ttttgaatca
     2221 tctgttgcgt caatatttgc gcccatttct ctaagtgggt taattaccct gcccattggg
     2281 cgttttttaa gatatttgtc gcctgtgata gaagaggaaa aattctgtcc gcttaatagc
     2341 cctataagaa gtcttgatgt agtacctgag ttaccagcat ttatcatatc ttctggctct
     2401 tttaggccat gcagcccagc accattaatc cttaatatct caccctgcca ggatatatct
     2461 actcccattg cttcaaatgc tttaatggta gagaaagtgt cttctgattt taagaatcct
     2521 tcaacatttg taactccatc tgcaagagag ccgaatatca ctgccctgtg agagatggac
     2581 ttatcgccgg gaagcgtaat ctcgcctttt attgaagtag gcccaccttt tatctgctct
     2641 cgtgccataa accctaaaac taccaccaag ggggtttatt aacaagttgc agtttcaata
     2701 gtggtgattt aacttataga gtctatttaa tgatatatgt ggcaaaaatt ataagctcaa
     2761 ttttttagtt catgttttat taaataacct atatagtatt ttagttcgtt gaaaagtact
     2821 ggttgtaatc cttttttcaa caaacttgct aatgcaacac ttttttgttc accgaatact
     2881 tcatcaatcc attttgttgt agaacaataa aactcccatt gggtttcatc aagagaatgt
     2941 gccttattaa tatttttttc ggtgtgtaca caaaatatcc atatatcaga atatctgcat
     3001 gctttaccta agtatttatt atgttctgga agtcagggat cttttttgct tatatcaaac
     3061 ttaattttac tataattgcc ttgttgattc catgtttgta aataacctga agtttttact
     3121 tcaattttat aatctttata taaaatatca tatgcatccc aatcaattct ctgttttgta
     3181 gaggaatcca gtgctttgtg aactaaatat tctcctacaa ctgctctagt tgtgttattc
     3241 agaagattgc cataggccca attaacaaaa tcatcaacat taaacattaa aatttaaata
     3301 ataaatacta ataaaatatc tggatttgat ataaaaggac tctaaagtat ttagccttat
     3361 tccaacggca gttcccttac aagatcatcc cagtgggctt ctgtaattgc ggctatctta
     3421 cccctcattt ccttatagct tccttcatca ggaaaaagct tgtctacaac ctcttctctg
     3481 cttgcaagcc acttgtcgat tgcatggtag tttttaaacc ggactactgt tttatgagtc
     3541 cagtgcggtt ttttggggtg gattctctgc gagtacattc taatatcatc ttcaataagc
     3601 cccatttttt tcatctctct ccaaaacgga aaaattcttt ctttataaat cttcataaac
     3661 tcttctttgt tttcatgggc gatcacatag tagctttctt caattactac tttgtatctt
     3721 tcaggtacat cttcggcatt ggatttttga ggtattgcaa aaagtgaaaa gcttattatc
     3781 agtaataaaa ggtatttcat aactaaactc ctccttaaat attgaaaata tagctatatt
     3841 acaatattta attaaagaga agtcaaatat taggcattaa tagaagtgta accggtttag
     3901 tttgagacaa taagaagaac taccgatgaa ttttgttttg gtttatattc taatgataca
     3961 gaggactctt gtctctgcca tacatagttt tgataaccaa aatcttcaat tttattaaat
     4021 tcattgccaa atttttggag tgcgtgttcc ttaagttctt tccagttttc agggccattg
     4081 gttttaaaag caacaaagta tagtttgtca ttattaaaac catattcaat gtctttaagt
     4141 tctaccccat tgtaatctaa attatcattt gtatttttgt agatcttgat ctcgccctgg
     4201 ctggttttac cactttttac aaatttcaaa ttgtctaaag aatctaattc tgtaccccat
     4261 ttaagagtgc cgaaaccgac cgtatgattt atttgttcaa cggaaaccgc agtgtaagtt
     4321 aggtaaaggt tggctagtaa aaagaaaatt gataatttta aagaaatagt tattattctc
     4381 atcatttttc tcccataaat caactatagt attaactata taatcaattt agtattaatt
     4441 ataataccaa tattaatgaa aataaataag actatagtct tgttttagac taaatgctga
     4501 attgtgttaa ggtatagttt gaatagttca catataatat gaagattggg ggctaacttg
     4561 caaaaatgcc tatgtttttt atgtcatgta aattatcaag atttttaata aaaaatttat
     4621 aatcgtaaca tattgtactt attgcatttt atttttatcg taaaaaaagt tcttgacaac
     4681 cacaacatat tgtgtttgaa tcgtattcag agtacaacat agcataaaat aatttattaa
     4741 gcatgagcta attaaaaggg ggatttaatg gcttcagaaa agatagacga atcacattca
     4801 aatggaaacg gaacagccgc cgtagaaaaa caaaaaagta tttcagctga catcgaagcc
     4861 agtactgaag ctaaaaatga cggtggatat acagaaggtt tagtagatat tcccgaagaa
     4921 accatcgaag ccttcggtgg tgatgagctt agagcaagag tattttttga aaaatacgcc
     4981 ctgagagaca aatccggcaa aatggtagaa aaatcccctg ccgatatgtg gagaagggtt
     5041 gcaaaagaga tagcatctcc cgaaaaaaca actgatctta aaaaagagtg ggaagaaaac
     5101 tttttctggc ttctaacaga tttcaggttc atccctggtg gaaggattct ttttggtgca
     5161 ggacagaaaa gaagagccac tcttctaaac tgctactata tgccgattaa agaggattct
     5221 atagaaggca tatttgaatg gtgcaaagaa gcagccagga cgtattcatt tggtggtgga
     5281 gtgggaacag acatttcaat cctaagacca aaaggcgctc cggttaacaa ttccgctatt
     5341 tattccacag gtgccgtatc atttatggaa ctgctctcga ctactacggg cacaataggt
     5401 caggcaggca gacgcggcgc tcttatgatc acactgggcg tcaatcaccc ggacattgaa
     5461 gaatttatag acataaaaaa tgacgcagac agggcaaaag taaattttgc caatatttca
     5521 ataaaaatca cagatgattt tatgagggcc gttgaaaatg acactgaatt tgagctctca
     5581 tttgaaagcg aaaaagtaaa agttactaaa aaagtacatg ccagaaagat ttggaacaaa
     5641 ctgatcaaat ctgcatgggc atcggcagag ccaggcgtga tattctggga tgctgtgaaa
     5701 agatattctc caaccgaata caatggaatg gaggtaaacg gtgtaaaccc ctgtagtgag
     5761 caggccctgg aagattacgg caattgctgc cttggaaata ttaacctttc attatttgtt
     5821 aaaaactcat ttactgaaga tgcttcaatt gattgggaaa acctcgaaga gacctgcaag
     5881 tattcagtta gatttttaga taatatcctt gactacaata tgcaaaagca tccgcttaaa
     5941 tatcagacaa atgcctctac actgtccaga agaatcggtg tgggttttac aggctatggc
     6001 gatatgcttg caaagctaaa cataaaatac gatacggacg aagctgttga atttgctgat
     6061 aaaatgtttg agcacattaa aaatacagtg tatgaagcaa gctctgatct tgctgctcaa
     6121 aaaggaagtt tccctgctta tgatgaggaa attcatttaa atcagccgtt tttaaaagct
     6181 atagataaaa atgtatatca aaaaattaaa aaacagggag taaggaacgc atgtatttta
     6241 acagttcccc cggtaggaag cggttcagta cttgccggaa catcaagcgg tgttgaacca
     6301 atatttgcac tttcttacta tagaagatca aaatcattat caaaagaaga atttaaagta
     6361 tatcatcctc tggttggtga atatcttgaa aagttcggat tagaggatga atcggaagta
     6421 ccggatgtat ttgttacatc gcatgaaata aaaccagaaa tgagagtaag aacccaagcg
     6481 gcaattcaaa agcatataga ttcatgtatt tcaagtacgg tcaatctgcc aacggatata
     6541 tcgcttaaag aagttgaaca gatttacttc ttagcctgga agctcggatg taagggtatc
     6601 acagtataca gagaaggctc cagagaaggt attttagtca ccaacgaaga aggtgttaag
     6661 cagcaacagg caaaagaaga agcagaacat ttccaacccc gggatgaagg gcaaaaaacc
     6721 ccacaacacc ccatctctac cccccttcat cccgaaaatg aaatcaagcc tgtaaaacgt
     6781 cctcagactc ttgagggttt tacagaagtg atcaaaactg gttacggcag tttgtacgta
     6841 accataaata cctatgaggg taatcctttt gaagtgttta tccaaatagg aaagtcaggc
     6901 tattcaacaa tggcagacgc agaggcaatc ggaaggctag tgtcgctatc gctgagatcc
     6961 ggcgtgaacg taaaagatgt ggtggaacaa ctcgaaggta ttggcggagc ttcccaggtg
     7021 tattccgaag ggaagctcgt catgtccatt cctgatgcaa ttgctactat cttaagaaag
     7081 cattttatgg acgaaaagaa agtatcaaag aaaacatctg atatagatct tgaagtttgc
     7141 ccggattgca gcagcaggtc gctagctttt gaatcaggtt gtgtaacttg cagaagctgt
     7201 ggttactcta agtgtgacta gagcattatg cttaggctca atagatgaga ctggataatg
     7261 tagtaattgt tttttttatt cattatgcac tttcagtgtt gatattatca acatcacccc
     7321 ttacttcttc agaactcata atctctgatg ggggctttat tgactcatgt ctgcggtttt
     7381 ttagagacta tctttttatc ccaaccagca cttttctaaa tatcacaggc atcagattcg
     7441 aaggttatat gaagtatata ccttatattt ttaacagtat tctttgggcg gttgttatta
     7501 attatctttt ggtgcttatt aataattttt taaatccgaa aagactatct tagaaattaa
     7561 tataggagga tgatgattta tgcttgatga attcaaaaag tttgccatgc gcggcaatgt
     7621 agtcgatatg gctgttggta ttgtgattgg aggggcattt gctcttatag tcaattcatt
     7681 agtgaatgat gtaattatgc cgcctgtagg tatgctgctt ggcaatgtag acttttctaa
     7741 tttgcatgct gtattaaaag agggtaccac ggcaggtcca tatatatctc ttgccgcagc
     7801 taaagaagcg ggtgccgtta ctttaaatta cggtgctttt ataaacacga tagttagttt
     7861 tattattgta gcctttgcta tttttatggt agtgaagggt atgaacaaga tgcaggaaga
     7921 agaggaagct ccagaagaac caaccacaaa ggaatgcccc cactgcataa gcacgatttc
     7981 cattaaagct acaaagtgcc caaactgcac ttcacaggtt taaacaataa atacatgatc
     8041 tgaaaataac ctgctatata ttctttagca ggttataata ggtcttgtat gaaaattgct
     8101 atttaccaga tagatgcttt tacttctgag ttatttcgtg gaaatccggc agcggtctgt
     8161 cctttagata aatggattga tgattcttta atgcagtcca tagccgctga aaataatctt
     8221 tcagagactg ccttttttgt aagagaggat gatggctaca ggataagatg gtttacacca
     8281 actacagaag ttgatctttg cggccatgcc actcttgccg cagcatatgt aattttcaat
     8341 gaaattgata aatctgttga gaaaatcaag ttctattcaa acagtggtga gctgaatgtc
     8401 acaagagatt cggatttaat aacattaaat tttccttcac tgccccctaa tatctctaag
     8461 aaacctgaaa aagttgtttc tgcttttaat atagaaccaa tggaagtgtt tgaagcggat
     8521 gattatctgc taatttatga gaatcaggag cagattgaaa acatcaaccc tgatttaaat
     8581 ttgttaaggg aaattgatct aagaggagta attgtttcaa cacccggaaa tgactgtgat
     8641 ttcgtctccc gcttctttgc tcctaaatat ggtatagatg aagaccccgt cacaggctct
     8701 gcacattgta ctctaactcc ttactggtca gaaaaagtaa agaagaaaaa actgcatgca
     8761 aaacagttat ctaaaagagg aggggagctt ttttgtgaac ttaaattata cagtgtcagt
     8821 atttcgggaa aggcggttct ttatttgatt ggagagattg aaatacattt atgagtttta
     8881 tcccataaat aaattaacat aaaaataaat gttagattta aactataagg ggacaaccct
     8941 atgaaaactt tgatcttcgg agcaggtcct attggttccc tctatgccca tctcatctgt
     9001 caagcgggaa aggatgtgac tattctcgct cgaggtaaac ggtacgagtt cattaagacc
     9061 aagggtattg agttggtaaa cgaaatcact ggggatagag gggcctcgaa gcttagggta
     9121 gttaatgaac tgaaggagga cgatgagtac gatctggtgg tcgtattaat aagaaaaaat
     9181 aagcttcttc ctgtgtttga ggttcttagg acaaatgcca acatcaagaa tatcctgttt
     9241 atgggcaaca actcgcttgg cttcgtagat tacttacggt acctacctaa ggagaagttg
     9301 ctattaggat ttcctggagc gggtggtgga ataagcgagc aagttgttca ttatgccgac
     9361 cgagaaaagc caaaggataa aagaagacca gtcaccattg gggaaatcga cgggaaaact
     9421 aaaagaagaa cacttgagat caagtctctc tttgagatga gtggaattcc tgttgatctg
     9481 gtcggggata tagatgggtg gttgaagtgc catgtcgcat tgataagtcc attgggtaat
     9541 gctctctata agcacaattg tgagaactat tcacttgcca aagataaaaa aacgatcaag
     9601 gtgatgatac aagctgtcaa ggaaggtggc agggtcctga aagctcttgg gtacacaaag
     9661 cgttaccctt ttaaattcaa cctgttctac tggttacccg agatgctgaa tgtgaaggca
     9721 gttgagggtc ttctcgaaag caagtttgca gaagtagcat tctcactgca tgccagagct
     9781 gcacaggacg agatgcgaga gctgtcagtt gaattccaga ctcttattaa caagacatct
     9841 gtagagacac caaatatagt cacgctaaga aactttatac tctgaaaaag aaatgcaaaa
     9901 acacttacac tcatttatac aaagtcctaa cctagagaac ttaaagagta cagcgatctc
     9961 aatgcaaaag gatttgaggc ggggagattt gggtgcagtt atagatgaac tgaaatttta
    10021 gtatttagtc ggcatctaac ttcaaattgt attataataa aacctatatg aaaatgaggt
    10081 agtcagcatg aacatcacag gcataatgat tttttgtgaa gacattaaaa acatgactgc
    10141 attttatcgt gatgttgtcg ggcttacgcc gctcgatact cagccgtttc ctgcacataa
    10201 attctttaaa tttgtttcaa aaaaaggggc tgaactgtgc ctgcacagcg gcaccaaacc
    10261 aaacggcggc aggcaaaaat taatgctcta tactgatgac atagacggat tagtaaagcg
    10321 tgttaaagca tataagccca gagttcgctt gcctgagcct gataaggacg gcaatatagt
    10381 tttcgatttc cgagatcccg aacaaaaccg gatccaggta tattctaatg cgatttatag
    10441 aaatagatat acttgagttg atgtaaagta attaatagga ttcatatgcc taaaaagtac
    10501 cttttttttc ttttagttct tttccctgca gcaatctttc tattatatta ttacggcaga
    10561 agtgtttggg tacctaagta tcgtgcatta gcggggcaaa ggacggttgc ggatgtgatc
    10621 tcaatatatg gcgagcaggc tgtagcacgt ttagcacctt atttcaaaaa tgcaggtgtt
    10681 atctatccac ctaaacaagt agtaatgctt gcaacaaagg atgataaaga acttgagctg
    10741 tgggcatcag acggagcaga ttttaaatat ataagaacct atgatataaa aaagacaagt
    10801 ggtaaattag ggccgaaact gagggaagga gatcatcagg tacccgaagg tatctaccga
    10861 attattggtc tgaatccaaa cagctcatat cacctatcat tgaaactgaa ttacccaaat
    10921 gattttgatc ttttacaagc taaaaaggac ggccgaacaa acccgggctc tgatattttc
    10981 attcacggta aagctttgtc tgtcggctgc cttgccatgg gagataatgc tattgaagag
    11041 ctattcgtat tagtggagaa tgtaggtaaa caaaacgtaa aagtggttat agctccattt
    11101 gatccgagaa agcaagattt agttgttcct gagggaagta atccaatctg gcttggtgag
    11161 ctttataacc aaattgagga agaatttata aaatttaatt agggtacgac tactacattt
    11221 attttgattt aactatatcc ttataaacca tcttctgcat ttcaatacaa agctctgctg
    11281 tacttttagc atattgagca gctttgaaat ccattacttc taatgctttg tctgctgatt
    11341 ttagtgcatt tgtgcagtca ccaatttttc tgtatgcagt gctcatgttt cttcacgcat
    11401 atgcatagct cgatttttgt gtagcattaa ctttttccat aatatctata ctaatttggt
    11461 aattagatat aacttcctgc catttcgggt tttcaaaata ggagtttcct aaagctaatt
    11521 gaaaattgta gtgccatggt gaatgaaaag gatttttatt ttgtttcgat aaaataagag
    11581 ctgcttctat catatcaagt ccctgcgcaa tattatcatc attatatgtc cacctacctt
    11641 tatctataag tattttacct ttgtttaata catagcgagc agactgaggt tccaggttta
    11701 tcgacttttc aatttcaatc agagcagtat catatgcttt atgtgctgaa gcatatacag
    11761 catttatata gtgataaaat gctctatttc ctttggatat tgcagtatcc aatatcttat
    11821 ttacttccgg cccaagcata gcaagatatg gggcttcttt gctcaagctg ctacttggaa
    11881 tagaatttag cttgattagt gttacctgcc acaatgcttc aagatggtcg ggttcttttt
    11941 gcagtatttc aaatagatat ctttgccttg ctgatgaatc attacctact tctgaaaatt
    12001 tagcatcaag tgctttttga agcagttgat caatctcgtc acagattgca atgttggaga
    12061 ataaaaggaa tacggttgta tttaatataa aacgtttgat cataatagta atcccgtaat
    12121 tgtatatcct gaccagaaat aagggtgatt gtattcttta tttttgattg ataataattg
    12181 tgccttttta agtgaagttg caggattatc acctgatgaa tatgatttat aaaaattttg
    12241 cattaaaaat gcggtgccct tgtcatggac agcccatagg ggggataaaa tagattgtgc
    12301 acctgcaaaa aacaatgccc tatgaaaccc aaaaacatct ctttcagtat ctatgtctga
    12361 tagtcctgtc tcacaactgc tgagagttac aagcgggaaa ttgccaagat cggtggttgc
    12421 aatatcatgt gctgtccaaa ttcctgattg agaattttca gacactgata aataaattga
    12481 agattccata ggttttccag agtagaaact cccatgtgcg ccaatgtgca gaaaagaagg
    12541 gattttaact gaacttattt catcggataa tccagaaatt gaacattttt tgcctgctat
    12601 caggtttaac ttagaattat atagattttt taaataatca ccggtttcag cctggaatgg
    12661 cagacgtgca cctcgaactt ctggatcaac acaagcaatt ccactatcta atgatttttt
    12721 atttaaattg caaaactctt tataaacctt aaagaaggga cacggtatat agaataattt
    12781 tctatcagaa atgattttcc gtcataaaga gggctaaagg ggatactgct taatacatta
    12841 tcagatacaa caataaggtt ctttatgttc ttgtttaaat gttttagtac ttcaccgaat
    12901 agagcactat ataattttac agaagattct ttgtaatagt ctgtataagg attaattatt
    12961 gaagctctta actcacttgc aagaagatag agtgaattct catcaatatt taattttaca
    13021 aactttttac tattattaag taaaataaat aatcctgaaa gttctttgct aatataaaat
    13081 gataaaattg cagtatcatc tggtagatga ttttgagcta aatgaatatc attagcagaa
    13141 agtgaactgc ttactggaac agttcttatg ttaagtgata tttcaagttc ctttttttcc
    13201 tgttgctgtt tttgagttat ctgagagtac ttgattaata tattttcatc tgatatttct
    13261 gatgtttgtg aattttgttt aataaagtct tgtatttcta gttgatgttt agtatttaga
    13321 tcggaaagct tttgataaac atccttttga taaacatctt ccattatatc atttaaagta
    13381 ggagctttat ttgattcgat agttacaaga gcatgcatat acttgttgtt tgttattaaa
    13441 tgatttatgt attcgtctat ataattatta aatttagaat ccaattttgc tctacctaca
    13501 tctcccaatg atttagatct agcatgctga taggctatat aagctttatg atatgaatta
    13561 tcggcctgct ctgtatattt cagttttgaa tatgacttag aaataagaaa taatgtttca
    13621 gagtacattt ccccctgcat ttcaactact ggaaattctt ttgtaatatg ttctaaaatg
    13681 tttagtgaat tttgataatt atctgttttt tggagataga tagaatatag tttgccagct
    13741 tcccatctgg cccatctgtc atatctagat tttcctaaag taagtgctaa atcccatgca
    13801 tccttataaa atatgccggc cttttcctta tgtccttgat aataatgttt ggatcctaat
    13861 tcttctagaa cactgactgt atcgcgttta taatgtgtgc cttttagatt atctcgggct
    13921 ttcattaacc acatgagtga taaactttca tttttgtaag ctagcatcct agatatactc
    13981 aattgcacta caccaagatc atggggggca tgcatagttt cagttatttt atgctgcagt
    14041 aaatatgttt taagagcatt ctctttatcg cctgctttat taagaatatt tgcaatatag
    14101 ccaagagaaa caccaatacc tcttaagttt ttattgttat aactaatggc taatacttct
    14161 tgataagttt tatatgctcc tttataattt ccccgagata attgttttct gcctttttta
    14221 ctgtctaagt ccatggcata tttcatatag ttgtttttgg ggttcaattt tatccctttg
    14281 aaatacgcct ttatttcttc tttcgttttc tcatcgatgt tgaaagtatt tctttgagta
    14341 ggtgaatgag gtttcttttt gtgctttttt ttagattgga atttggattt tgattcgcta
    14401 ggaaagaaat cattaagata tggtatctcc gtcctgtaca tgtatacagc ggtaccaagt
    14461 gcagcaatta gaattagtag aagtcctatc ctcattgaat tgtgagatta aaatatgaat
    14521 aatatgtaat taggaaatag taattcattt ttaataaaat atctatagtt attttacaaa
    14581 ctattggtat caagtcacaa atatatgaat aaatatattg aatatgtcct agatttttgg
    14641 tttggcgata taagagatgg tttaatagta gaaaacagaa atgatttctg gtttatgcca
    14701 aattctcaga ttgataatac tatcagggaa gagtttgaag agcttgtggt taaggctgga
    14761 aaaggaaaat taatacactg ggagcagtcg gctaagggaa ctttggcact aattatattg
    14821 ttggatcagt tcacaagaaa catatacagg ggcaaaaggg aggcgtttca gttcgatgct
    14881 gaagcactta ggatttgtaa agccggaatc caaagaggtc atgatagaga gttggaagtt
    14941 attgaaaaat gcttttatta cttaccactt gaacactctg aaaatctgga agagcaggac
    15001 aagtgtgtga aactatttga ggatttgctg gttacagttg atagcaacaa ggtaagtatt
    15061 gtaaaaaatt ccttggatta tgcagtttta cacagaaata ttatagagaa atttggaaga
    15121 tttccacaca ggaatgaagt actgaacaga aaatctacaa atgaagaatt agttttttta
    15181 tcgaaacagg gtaataaatt tggacaatag aataatttgc tataattata taaacaaaga
    15241 tttttaagga gatagatttg aataataaaa taatattagc tgcgtttagt ctttttctca
    15301 catttacagc attttctatt gccgatgaaa cctcacacaa aaaagccgca caagagcttt
    15361 tggtaacaat ggacattcaa aatatgcttg agaagtccaa taaaagaatg gtagccatac
    15421 aggttgcaag aaataaaaga atttctggat atgaagaagt attaaacaac ttttataata
    15481 aatactctaa ctgggatatt gtgaaagatg atgttataga attgtatact ataaaattta
    15541 ctgaagctga gttaaatgaa cttgcaagat tttataaatc tccagttgga cagaaagcac
    15601 ttgctgaagt cccaactcta atgctagaaa ctattgccgc aggccagaaa aatatagaaa
    15661 agaatctacc cgagctgcag caacagatac aaaaattaca ggaagagaag aaaaagaatt
    15721 aatataggta tagtttcata aaaacaccaa ttaaacaata ttgagatgtg attatgaata
    15781 gtataagcaa tcatctaatc atacttgctc ttttcgcatg ctctttctac ataagtcctc
    15841 atagttattc tcttacaata gaagaggctt atagtgcaat acctcataga agaacagagt
    15901 ttgattacaa ctctgcaaaa atgtctttag aagaaaaaac ttacttatca gaagtatttg
    15961 aattaactga tcttgctacg atcgaaaaag ttaatatgtt gatgtggctt ggcagcaacg
    16021 gacaaaaagg cgaagttgct aaacattata gtaatataat atcgagtctt tataatctaa
    16081 atccaccgtc cggattagac aattttcata atctgataat cagttccatt aaagaacaaa
    16141 aagaaacgct ttttaaatgg aaaatcagtt cgtttgatta ttcgatgatt aggagtgaga
    16201 gtttagttag aagttcaagt aagaaactaa agagtgcata tagaatacta aagaaaaaat
    16261 atcccggtga gagtagctat aacaaacggg ccttctttga tcatctttgc gctttggact
    16321 ttatataaaa tatcattttc atttgttact tctttatagt aaattagcta aatgccttcc
    16381 ataaaagata cgtccaatca gatgattcag tttgtatttc cggagcatgc aaacaactac
    16441 ggcactttgc atggcggaag actaatgaac tggatcatgg tgctgggtag catcacttca
    16501 tcgagaattg caaaggggct tattgtactt ggcactgcag acagcattga tttcctaaac
    16561 cctgtgagaa caggtgaggt agtgttgtta aacagctggg tagaatatat cggtacaagt
    16621 tctatggaga ttggtgtaaa ggtagagtca gaaaacacga gcaccgggga gaaaaagctt
    16681 actacatttt ctcatttagc atatgtagcg atagatgaaa atggcagtcc aaaaaaagta
    16741 gaaaacaaaa ttagtccggc agatgatgat gaaaggaagg tctataagga cgctaaaaaa
    16801 agaaaagcac aaaggattga gagacttaag gacactaaac cagaattaaa ggatattaag
    16861 tatctaaaaa attatgagtg gcagatagaa actataagag ttgtgcttcc agaagatgca
    16921 ttttatggaa attatctttc agttggaaaa ctaatgatgg atattgatga aaccggggcg
    16981 atactagcaa gaaaatttac cagaggtgtc gttgtaacag gttcagtgga tgatgtgcat
    17041 ttttacgagc ctataagggt tggagaactg ataacactgc atgcaaggat aaattacatc
    17101 ggcaggtcat caatggatat agaagtaaat gtgcatacag aaaaccttac aactgctgta
    17161 aaaaaacatt cgtgcacggc ttatctcaat tttgtgcata ttagtaaaga gggaagacca
    17221 aaagccatag ataagataat tactccttca acgaagtatc aaaaaaaatt atggaaacaa
    17281 tctgaggatc gcaaagacag gagatttgaa cgggtaaaat ggatcaagaa caatatggtt
    17341 aatgacaatt agtatgatta catttcctac tgtattttat caattatcgt agtaacctac
    17401 tttattaaac tgctcatctg taagctctat atttataggg ttttccagct cataaaagaa
    17461 ctcctcatta ctattttcga ctttgctaaa tgcccttatt tttatatttt ttgatttaat
    17521 aaactcagaa ggaatgctaa ctacccatcc agccaggatt gagcctatat aatgataaga
    17581 tttgttaaaa ctcagacatg gttttatagc tttaataatt ttaccgttaa cagatatgta
    17641 ggtgagactt gctggattta attttggatc taccgagtat cctcctagag taagaatata
    17701 gtcttgtgaa ttttctgttg gcaatgtctt ctctatcttt aaatactcga gcttgccgga
    17761 tttttgtctc attaggggaa gctgttcagc atttttatca atccattttg atttataaac
    17821 agtgagttta ttaaatttat gttttattgt atcaaaatat tttaatggct cactgatttt
    17881 accttttaaa aatgttccac tatccgtctt gtttttccat tgattatcct catacattat
    17941 ctgaaaaaag tccaggggtg gtgaaaggtt tttccaattg tcagaaagaa gggattgtcc
    18001 gtcgatatcc catggtggat caaactctat aatgtccatc aaagtggcac caatatctat
    18061 aggtcttacc attctatagt ctgtttgtgg ctgtttttga tgaggatatt taatcataag
    18121 taatgtagaa gcattcatca ttctgttatc cagtatatct tcactatttc ttaatttatg
    18181 gtttgccctg ccaaatgaag gtcctaaaaa tcccacacca tggtcactaa ctgcgataat
    18241 cagtgattta tcaaataatc caagttcctt aattctggta attgttttat taaacatctt
    18301 cattgcaagc attgattgat taattcttgc tcttctgatg ttgatggaaa tttcgtcact
    18361 agtgcagctt gtttgagcat attccgacat cgctacggca tcgggcagat aattgacttt
    18421 ttcattatca tccaataagg gtcttatagg atatacaacc tcaattattc catcagagtc
    18481 aaaacaaaga ggggtatgtg taaggctggt aagaatactg ttgataaaat atctttgggg
    18541 atcaattctg tcagtgaaca aattaaacct gattatggct ctgtttgctt ttgtggcaca
    18601 agctaaaaat ccatatgatt taggaatacc atcaacgata gcaccctttg cgtgatttga
    18661 acttgttagg tctaaataag tatcagcacc aaatatcttt actgaccagc tggaattttg
    18721 tattgataat agatataaat ctatgatctg tttaaggaaa ttctttaaac tgttattcaa
    18781 cccaggttca attaaaattc tgagtgtatc tttttgaacc ttatatttat ctattgatgt
    18841 ccataaatca atcttattag ttttttgttt tataccattt agattgcgta tatcttttgt
    18901 agggatttta cctttaacga gattccagat aacagtatgt ccacaaagtt cctcactacc
    18961 actgcaattt ttaagctcat catgtcctga aaacattgag cttgtagttg ttagagtttc
    19021 ggatacctgt gtgaccgcat ttgggtacca tgttgaaatt ttcgcaaatt catttaactc
    19081 cgggaataag gctttattaa tttgcttatt ctcatccatt aatatatatg aactcagttc
    19141 atcaaaaatt ataaaaaata cgggagtatt tttgtttccg atcttactaa aataatcaac
    19201 ttcgttttta tttattattg ttgacttgtt ttctttaaga aaattatagt attgataact
    19261 aaaatacaga gtaagcgggg cggatactaa tgttagaatt tgaaaaaaag tgaatataaa
    19321 acgattaaaa cgatatgcag ctaagaatag aatgatgaaa aaacaaccga ttatgaggct
    19381 tgagggtgta taaacggaat ttaacaaata attagctcta agagaatata gagtaatact
    19441 gacagcaatt ataatacata gatatatcat ggatactctt ttatttataa aacttaatat
    19501 ggtaaataat agagttaata aaattgttgg aatgattaat ataagtgtgg gtagagtaag
    19561 tgaattaact agaggattta aaaatggcgg agggaactct ttaaaatccg atgaaaatac
    19621 ctggaatata gtaattccta taccaacata gaggcaggaa aataatatca gcaacactga
    19681 cagagtatct tttgaactgt gtttttttag ctcagacatt tagaattgtt attaattgtc
    19741 attatgaatt tatagtattt acataaaaaa agtaaagtac tataatgatt agtaatgacc
    19801 ttccccccta taatagtacc agattacaag ttagaatttt aggtaataaa ccctatgaat
    19861 aggaggcaag ggaaatgccc
//
LOCUS       DBNGSV010000160        21715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_166696, whole genome shotgun sequence.
ACCESSION   DBNGSV010000160 DBNGSV010000000
VERSION     DBNGSV010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 21715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21715
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_166696"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="AC159U_05650"
     CDS             complement(<1..739)
                     /locus_tag="AC159U_05650"
                     /inference="COORDINATES: protein motif:HMM:NF028512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3336575.1"
                     /translation="MSRLFKRTLIIIIILFGVIATLTSALSGWNIYNGLSKEFTSKGL
                     SIATSISDSSVEILLNRDLSTLQSVIDQYLKIEGVSYVLVENNEGEIVSHTFVPSVPA
                     EVIQRNTKNNDSFLKEKHTIRDLTIKGRGEFIDISVPILGGVAGQVQVGMDKSIIKNE
                     IVSIIAKQQALMFVIFLFTIGLSYFFINRISKPLNALTDQVKNITTKELTVQNINESQ
                     IKSIADNSQDEIGDLARSFINMEVELVD"
     gene            complement(742..1950)
                     /locus_tag="AC159U_05655"
     CDS             complement(742..1950)
                     /locus_tag="AC159U_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015783429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3336576.1"
                     /translation="MTRSFYILFNFVLSVVFLSSVWADDNKEPIVIGMSAAFSGQSKD
                     LGNELHRGIMTYLDYVNENGGVDGRKVVIKKYDDGYNPIPALRNTLKLMKEDNVLLLF
                     SYVGTPTVTRVLPLLKLNSSDHFYLYFPFTGAQPQREAPYNDFVFNLRPSYRDETAGL
                     VKNFINIGKKNISVFYQADAYGRSGWAGVRKELNKHGLNITAEATYKRGSEYSDSYKK
                     QVGILKEASTDAVVAIGSYAACAGFIRDARDAGWNVPIANLSFVGSESMLDLLVQQNK
                     TGGKDHTVNLINSEVVPNYRDTSIPVVNEYRKLSGINNNSSDNDLSFVGLEGFLNAKL
                     LVHVLTVMDGKIERKHLKQVTESIRDYDIGLPDKISFGTGKHQALDTVYFNTVKDGKW
                     ININNFESFR"
     gene            2014..3768
                     /locus_tag="AC159U_05660"
     CDS             2014..3768
                     /locus_tag="AC159U_05660"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3336577.1"
                     /translation="MGNIIEGLNITFSELFRYIISGSHNELSSVIFHAVILLIILILQ
                     NKIRTTVDTHDFAKERYFLFIIDLLFPLIFLAFVLYIAPEVLSDKVSQILFNAIGQLY
                     FAFFFIRNLIKLKSFEYIPWGVLIYVLSIISVFAYLVLNLNASFSNNAETNEFFLIVL
                     KISLMLLFYTFFYRSTGNLTGYISDKRILAQRLVENLIPFISVLFLIVSVLWLLEIIH
                     ISSNIFRGLVLCIIIILVYAFLRAYSTAQLATKIFSQSESYLELLSKLQLLLKLLLVY
                     GLYKIFLTFFNFGYFVHYLQSLFILKTDLVKISFFSAISSVFLFLVLFSLIGVFKNIA
                     FLSYNKRGKEIEAGTTRAIISNLGLLLVIIISLSQLGLNWKVLIPFAGALGIGIGFGL
                     QTIMNNYISGFILLFSKKLKVGDVIEIEGNAGRAIGNTLETIYGRVESIDVLSAVVKT
                     TDNIEIVIPNSQFIEGQIVNYSLSDSDIRLRIPFGVSYASDPNQIKEILLKVSKDHPS
                     ILSSPAPNVWFKEMGDSALVFNLLCWINIRMLWRINPLISEIYFKGWYELKEAGVEIP
                     FPQRDLWLKNKIKVEIEK"
     gene            3817..4152
                     /locus_tag="AC159U_05665"
     CDS             3817..4152
                     /locus_tag="AC159U_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3336578.1"
                     /translation="MKKLKIIISIVISYWLLCPLHSSASDVLNLTKEQKAWISSNPTI
                     IVGNEIDWPPFDYAEDGLAKGYSVDLMRLIAKKAGLGIEFVNGYTWQQLMQKFKAGEI
                     DVMPAIYLT"
     gene            4210..6651
                     /locus_tag="AC159U_05670"
     CDS             4210..6651
                     /locus_tag="AC159U_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3336579.1"
                     /translation="MVNKNSKYISDLEQLSGKKVAGVNGFSITKTLMENYPNIEIIPV
                     TSTVDGIKAVSVGKAEAFIDSIGFVSYILENNFVPNVKIITDADLKEVSNPALHIGVS
                     KKNGILRDIIDLAFKSISSEEEKLLHDRWLTSSLIEKDAILKKSELNLTKKQKKWLKE
                     HPVIRLAPDINWPPFEWVDENNEYKGLASDYMRLIEEKLGIEFYVEKDKLWPQVVEDV
                     KDKRLDIFPCVASNPQREKYLNFTRPYLSFPMVITTLDTSGYIDGVNRLKHMSVGVVE
                     GYATHEYFEANHPDINVKTYKSATDGLKDLSKGKIDAFVGNLATTSYLIPKIGLTNLK
                     ISGEMPIRYDLGVGIRKDWPELAAIMQQAFDSITDEQKNEIHNRWIGVRYEYGFDYSL
                     FWKILGVFVLVLGVLYFYNRKLAEEVGRRKFAEQELKHMNKELLEARDVAQSANMAKT
                     DFLSRMSHELRTPLTAIIGFAELFSKDNNLDEEHKKDAYIIHSAGQHLLNIINDLLDL
                     SKIESDNIEIEMKDISLNKIVNECFNLIKPLAAEHDIQVINNVTTNSAHMIYADYTRT
                     KQVLINLLSNAVKYNKSGGTITISHEIVDDRIRIKVIDTGKGLIKQQIEKLFNPFDRA
                     GAEDSQIKGTGIGLVISKRLIERMGGSIGVESEPGQGSTFWIDLNLSQSETPSVIKEN
                     PIDIVKKSTTNGAKTILYIEDIEPNIRLVSHILKKHTNHNLITASSAKIGLEMANVDN
                     PDLILLDIGLPEMDGYEVLKHLMDNEKTQHIPVVALSANAMKVNVQRALDEGFKDYLT
                     KPIEVKNLLTCINNI"
     gene            6908..7066
                     /locus_tag="AC159U_05675"
     CDS             6908..7066
                     /locus_tag="AC159U_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336580.1"
                     /translation="MHFYKRWKYKKDNSIGKIRYYENELAKKDYDEGSIIEIDSDEDD
                     SQPPKYLH"
     gene            complement(7073..7222)
                     /locus_tag="AC159U_05680"
     CDS             complement(7073..7222)
                     /locus_tag="AC159U_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336581.1"
                     /translation="MLIIIGGAFSMLLGSGGIIGIVLFQKPEMKSAYMYIGLYGSEKQ
                     IGDLS"
     gene            complement(7332..7652)
                     /locus_tag="AC159U_05685"
     CDS             complement(7332..7652)
                     /locus_tag="AC159U_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336582.1"
                     /translation="MNEKIYYFIVSIPKESHIEFVTKLNSFILKQFPGKKNPYTIRPL
                     EIRDNDTLSKVTIKTTEENITRFSDSLFEKDYLFLEQNNDEIEIVRKDSVEFNLDDNS
                     FTRH"
     gene            complement(7719..9014)
                     /locus_tag="AC159U_05690"
     CDS             complement(7719..9014)
                     /locus_tag="AC159U_05690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3336583.1"
                     /translation="MNDINSTSQSLNNEIASLTKRVEKLEKIREISCKLSSSLRLDPL
                     LKEIVTTAAEVIDCAGTSIFLVDENTDDLRFKATTIKALNLISDVPVPLNESIAGQIY
                     LTSKPQIVHDVKSNRRFFDKIDKILGFESKSLLGVPLIINEKCIGVLEAVNKRGDDVF
                     VEDDIDTLTALAAHASIAIDNARLYESVLDTAAELEQKVEERTTELRLRNEELSSYDH
                     TVAHDLKNLLSIIISYSKILEDDFPLVSEKELGDSLQKITDYSFKMSSVIDEMLYLAE
                     VRDTDVQKENLDMAEIVEEAKKRVDFMLKEFNAELKTPDSWPGALGYAPWIEEVWVNY
                     LSNAIKSGGQPPIIEIGSTGLSNGDIEFWIKDNGNGISAEDQKGLFTRFMPYDQYRKK
                     GHGLGLSIVYRIIHKLGGTVSVQSEVGSGSKFNFTLPAN"
     gene            complement(9266..10129)
                     /gene="purU"
                     /locus_tag="AC159U_05695"
     CDS             complement(9266..10129)
                     /gene="purU"
                     /locus_tag="AC159U_05695"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR3336584.1"
                     /translation="MTNNKLSAILLFSCPDKPGLVSKISHFIFEIGGNILDLVEHVEI
                     DEKFFSIRIEWEMDGSSISPSDLNEKFKPIVDQFNAQWKINFKDKKKRVAIFVSKYDH
                     CLQEILWRHSTGEYDIEIPLIISNHPDLAPLADHYSIPYHLFEISKESKQVQEQKELD
                     LLRESNIDTIVMARYMQILSPVLIDEYPNQIINIHHSFLPAFIGGNPYKQAFERGVKI
                     IGATSHFATENLDEGPIIEQDIIRISHKDSIEDLKRKGRDLERMVLARALHFHFEDRI
                     LVRGKKTIIFE"
     gene            complement(10194..10826)
                     /locus_tag="AC159U_05700"
     CDS             complement(10194..10826)
                     /locus_tag="AC159U_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3336585.1"
                     /translation="MTQLDWYFDFISPFVYLQFTQLHRIPDNININYKPVLFAGLLKH
                     HEHKGPAEIPAKRKLTYRHIVWLARKNNIPFKMPPAHPFNPLKALRLSIALDNNIDAI
                     TKIFNYIWVNGQSLEDEEAWDKLAKELGVENPEDMIKQPQVKEQLRTNTEQAVNLGIF
                     GVPTTIINGQLFWGFDTTDMLLEYINYPDMFEEKEMSRVFNLPEAVQRKT"
     gene            complement(10833..11321)
                     /gene="rraA"
                     /locus_tag="AC159U_05705"
     CDS             complement(10833..11321)
                     /gene="rraA"
                     /locus_tag="AC159U_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HIR3336586.1"
                     /translation="MKYQTPDLCDEYTDLVRVVEPIFNSYGGKSSFGGQIVTIKCHED
                     NSVVKQTAGTNGNGKVMVVDGGGSLRRALLGDLIAADAVKNGWEGFIIYGCIRDVDAI
                     SEMDLGVKALNTVPLKTEKKGIGELNISVTFGGVTFNPGEYLYADNNGIIVSSKPLDN
                     KG"
     gene            complement(11330..12109)
                     /locus_tag="AC159U_05710"
     CDS             complement(11330..12109)
                     /locus_tag="AC159U_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3336587.1"
                     /translation="MEQILNLDNIVSLGLLILLQAVLGFDNLLYISLESKRAPAEKQA
                     MVRRLGIGIAVVLRIVLLLLLLKLIQYFQDPLFGIHTSIIEGTFNLHSIIVLLGGVFI
                     IYTSIQEIFHMIGLEDLGKEEKEKSSVSMIIFWIVSMNLVFSFDSILSAMALTKVFWV
                     MATAIIIGGALMIWLSDSVAEFLERNRMYEVLGLFILFVVGIMLLTEGGHLAHLTIMG
                     QAITPMSKTTFYFVITVLVLTDLVQSRYQKRLLKVKQIGVK"
     gene            complement(12264..12680)
                     /gene="msrB"
                     /locus_tag="AC159U_05715"
     CDS             complement(12264..12680)
                     /gene="msrB"
                     /locus_tag="AC159U_05715"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3336588.1"
                     /translation="MSENEVLERTGLTKEDWKKKLTKLEYHVIWEKGTERPFTGDLLN
                     NKKKGVYVSAACKQPVFHSDHKYESGTGWPSFYQPISEDSIKLIKDTSFGMTRWEVVS
                     SKCGEHLGHVFNDGPAPTGLRYCLNSAALDFVEESE"
     gene            complement(12820..13566)
                     /locus_tag="AC159U_05720"
     CDS             complement(12820..13566)
                     /locus_tag="AC159U_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3336589.1"
                     /translation="MSKLLVFRHVAYEILGTLDPLLRNAGFRIKYVNFERHPDAIPNI
                     DNYDGLIVLGGPMNVDQTDEYPNLLTEIDVIRKAIDERKPVLGICLGAQLLAKALGAK
                     VNKNKKPEIGWYDVTPTSNGKKDDLISHFAGTEKIFQWHGDTFEIPKGAVHLASSKLC
                     KNQAFKYGENAYGFQFHLEVDTNLIERWLNVPAHKIEIKNTNGEIIPENIHEQTPKHI
                     DRLIELSNLVFGQFFAHFELKGKVHTLPSR"
     gene            13765..15360
                     /locus_tag="AC159U_05725"
     CDS             13765..15360
                     /locus_tag="AC159U_05725"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3336590.1"
                     /translation="MPPNNDKGNKGGKGKNSGDSGVPTSVVALNKMGFGPRPGDIADF
                     EALSKSEKKRLDNYIDQQTDPSSIDDTELDTIISDYQSAGYFTTINKTRTQLWQEHVK
                     SSMGSDQVKPVRELELLTLIRATHSKKQLVEVLADFWHSHFSIYFGGSPERSMSIHYD
                     RDVIRPHVLGNYRDMLEAVSQSTSMLYYLDNYNNRASGPNENWSRELLELMAMGTDNY
                     FGSIPPEDVPGYPNSLGYVENDVIEVTRCFTGWTVANNTSRGQTGEFYYQESWHDTDA
                     KLVLGQQIPAGQGPLQDGLDVINILAHHEKVAEYIVFKLCRRFISDSPPADVVAAAAN
                     TFLSNADSPQQLKLVMETILKHSAFKSSYGGKFKRGFEFTASSLRTLGADFSVLIDES
                     WLNSFINRYRLIGQHLFHWKPPNGWPDVAGAWQNSNSYVMRWRLANWFIDRKIGDTLA
                     IDVIGQAPQTEQSATEIVDFWLAELIGYTGIAEADRTEMINFMADGGDPDVLLDFPGN
                     NSIRDRVRSLIALIQMSPEFQMK"
     gene            15391..16674
                     /locus_tag="AC159U_05730"
     CDS             15391..16674
                     /locus_tag="AC159U_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3336591.1"
                     /translation="MADFSRRQFLVGCSAAIAAMAGSRLTSVVMGDPLAEPDQEIMLT
                     IFIRGGWDALNIMPPFDGADRGFYEAARSTIQVPADSTYQLGTNNFFFHPSAARLHEL
                     YNAGYLAAVQATGILNLTNRSHFDSMSFMELGTPGSKVGTSGWLARHLESANIFAGPE
                     EQASLATGNLSPTSLLSYLDTLTIANPNNFTLNIGPSAWRTEQRQALTSIYGGESQVE
                     VTGSNAIASLDIVANNLLGSYTPEPGVNYPGGGFGRDLQTIARLIKAQVGLRSATLDF
                     GGWDTHNNQGPGGGGYFANHLQTFSEGIYALFNDLNTSTTDYRQRFTMAIMSEFGRTF
                     NENDSLGTDHGKGNAMFVLGGCVNGGQLYGSWPGLSGEQLHQGREVDVMTDYRQVLSE
                     ILIRRFANPNLSTVFPGYSGYTPLGVVMGDDITPT"
     gene            complement(16706..18406)
                     /locus_tag="AC159U_05735"
     CDS             complement(16706..18406)
                     /locus_tag="AC159U_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3336592.1"
                     /translation="MNNVDSDKSYLFAQVNPEPVLRLDSKGNVLVANEGSKKLFKKDE
                     IEGLSWFELCPGTTKGIFQKLIKENKSDQLEFEINSKYYQFNIRPIPEYGFVHLYGTD
                     ITKRKKTELVLKENISEVERKNRFVSIINSISTNVHQSISLGEVLERTVQSLSENLHY
                     LHLISIYIIEDNCLILKAHSGLPDWLLEKAKIIPYPNGYMWKTIAAGVTRYSPDATKE
                     EVIGKTSERLNINSFLCIPLRDKEEIIGALSVSSKNKYAFESGEQELFKIVVSQIEDA
                     ISNARLAEERQNLIGQLEENLEKIKSIQKELITQEKLASLGTLTAGIAHEIKNPLNFV
                     NNFSEIALELTEELNEALDKEKDNISEETYLDITDIIENLQSNSKKINEHGKRADRIV
                     HTMLQHSRDKKGDKQLTDINTLLDDDINLAYHGMRAQDDTFNVSINKDFDTSIEKLSI
                     VPQDISRVLLNILTNAFYETSKKYKMRGSDYSPEVRISSNNLENEVEIRIKDNADGIP
                     VEVQDKLFTPFFTTKPSGEGTGLGLSISYDIIVQEHGGTLSFETEEGKFTEFKITLPK
                     "
     gene            complement(18468..19664)
                     /locus_tag="AC159U_05740"
     CDS             complement(18468..19664)
                     /locus_tag="AC159U_05740"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3336593.1"
                     /translation="MCQTNQMTENGLPANILVVDDEPDLELLIKQKFRKKVKNNEFNF
                     TFANNGLEALNRIQEDKEIDIVLTDINMPEMDGLTLVSKLTEQYPTLRSVIVSAYGDM
                     GNIRKALNFGAFDFITKPINFEDLEITIDKTIQEVSGLKEAEDNRQKLTKIQDELNIA
                     YQIQSSMVPRVFPPYPDRNEFDIFAKMYPAKNVGGDFYDFFLIDPYHLYFLIGDVSGK
                     GVPAALFMAMSRTLIKATAMRGIPPDVCLAEVNKLLSLDNESSMFVTVFAGVLNTFTG
                     EVIYCNGGHNLPYIIKNSGEVLSIENTNGVALAFMNEENLYSSKSYNLSPGDKIFLYT
                     DGVTEATDSDDFFFTDERLEKFLNDHKQDSITDLVNKTVEEVYSYSTGTTQSDDITAL
                     CVQYMK"
     gene            19745..20254
                     /locus_tag="AC159U_05745"
     CDS             19745..20254
                     /locus_tag="AC159U_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3336594.1"
                     /translation="MDVFECVSTLSSVRNFIDKQVSDEVIMKVMESARLAPSAHNDQP
                     WEFILVKDKKVLKKLGDYCISGRFVSQAGFAVVILTDQSSKWFQIDTTRAAQNMALTA
                     WSLDLGTCWIGRLDTGGIMSLLNIPNRWQVLTVLPFGYFNKTYANTTKFRKSPQEVFH
                     LDGYGNKLS"
     gene            20327..20560
                     /locus_tag="AC159U_05750"
     CDS             20327..20560
                     /locus_tag="AC159U_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HIR3336595.1"
                     /translation="MATLNISLTDSMRKWIDEQVKSGEYANASDYMRDLIRHDQREHE
                     VIRLALIEGERSGISNRKVSDIVAATKGSLKNG"
     gene            20553..20843
                     /locus_tag="AC159U_05755"
     CDS             20553..20843
                     /locus_tag="AC159U_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3336596.1"
                     /translation="MADYELSGKADQDLTEIYIYSYNNFGESKADSYFLGLEQSLIKL
                     ADKPNLGRKIDYIKQGYFRYEYVSHSIIYKQKEAGILVMRILHGSMDMERDL"
     gene            complement(20857..>21715)
                     /locus_tag="AC159U_05760"
     CDS             complement(20857..>21715)
                     /locus_tag="AC159U_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HIR3336597.1"
                     /translation="AFGVTKKSANAYELSIEQILSMLNEEIREVHIVGGLHPDWEFKH
                     YLEIVKSIKDSYPETHVKAFTAVEIDWFCEISGKSLTEVLEILKEAGVDALTGGGAEI
                     LHPDIRKKICAPKTIATRWEEIHKTAHSLGIPTNATILYGHIEKPFHVVDHLERLRNV
                     EDESPGFFAFIPVLFQPDNTGLKKDVKFFPASYDMKVHALARLYLDNFPHIKAYWITL
                     GEKAAQVALHYGCSDADGTIMREKIIHDAGAPSELGHSRDFMVNMIKNAGYTPVERDA
                     LYNVVRVYN"
BASE COUNT         6669 a         3896 c         4072 g         7078 t
ORIGIN      
        1 agtcaactaa ctctacttcc atatttataa atgatcttgc caaatcgcct atctcatcct
       61 gagaattatc cgcaatgctc ttaatttgtg attcattaat attttgaacg gttaattctt
      121 tggttgtgat gttttttacc tgatctgtta gagcatttaa cggtttagat attcggttaa
      181 taaagaaata tgaaagtcct atggtaaata aaaatataac aaacattagt gcctgctgtt
      241 ttgcaataat ggaaacaatt tcattcttga taatactctt gtccatacca acctgaacct
      301 gacctgcaac accgcccaat attggcacgg atatatctat gaattctccc cttcctttaa
      361 tggttaaatc tcggatcgtg tgtttctctt ttaaaaagga gtcattgttt ttagtattac
      421 gttgtatgac ttcagccgga acagacggga caaatgtgtg agatactatt tccccttcat
      481 tattttcaac aagcacatac gatactcctt caattttgag atactgatca atcactgact
      541 gaagcgtaga tagatccctg tttagaagta tttcaacact cgaatcagaa atactggttg
      601 caattgatag ccctttactt gtgaattctt tggatagacc gttatagatg ttccagccag
      661 agagtgctga ggtaagtgta gcaataacac cgaataggat gataattatg atcagagttc
      721 ttttaaacag tctagacatt tttatctgaa tgattcaaaa ttattaatgt taatccactt
      781 tccgtctttt acagtattaa agtagacagt atccagtgcc tgatgtttac cagtgccaaa
      841 tgatatttta tcgggcagtc cgatatcata atcgcgtata ctctcagtaa cttgtttcaa
      901 gtgcttcctt tcgatttttc catccataac tgtaagcaca tgaacaagaa gtttagcatt
      961 taaaaaacct tccagaccta caaaactaag atcattatct gaactattgt tattaatgcc
     1021 cgaaagtttt ctgtattcat ttactactgg aatactagta tcacgataat taggtactac
     1081 ttcagagttt ataagattaa cagtatggtc tttcccaccg gttttatttt gttgcactag
     1141 tagatctaac atgctttcac ttccaacaaa tgaaaggtta gcgatgggca cattccagcc
     1201 ggcatctctt gcgtctctta taaatcccgc acaggcagca taggagccaa ttgcaacaac
     1261 agcatctgta gaagcctcct ttagaatgcc tacctgtttt ttgtaactat cactatactc
     1321 tgaacctctt ttatatgttg cttcagctgt gatattaagg ccatgtttat ttagttcttt
     1381 tcttacccct gcccatccac tcctgccata tgcatcagcc tggtaaaata cagatatatt
     1441 cttctttcca atattgataa aatttttcac caaacctgca gtttcatctc tgtatgaagg
     1501 tcttagatta aaaacaaaat cattataagg tgcttctctt tggggttgtg ctcctgtaaa
     1561 tgggaaatat agataaaaat gatcagagct atttagtttg agcagaggca gcacccttgt
     1621 tacagtagga gtgcccacat agctgaaaag aagtaataca ttatcttctt tcattaattt
     1681 gagggtattt cttagtgccg gtattgggtt atacccatca tcatatttct taatcacaac
     1741 cttcctgccg tctaccccac cattttcatt tacatagtcc aggtaagtca ttattccacg
     1801 gtgaagctcg tttcccaggt ctttagactg accggaaaat gcggcggaca ttcctattac
     1861 aataggttct ttgttatcat ctgcccatac tgaggagaga aaaactactg atagtacgaa
     1921 attgaataaa atgtaaaatg atcttgtcat attttaattc agactattta attaataaca
     1981 atttatatct ttatagtata atatcaaaca accatgggta acattattga aggactgaat
     2041 ataacatttt cagagctttt tagatacata ataagcggat cacataacga gctctcatcg
     2101 gtaatttttc atgctgtaat tctacttatt attcttatac ttcagaacaa aatcaggaca
     2161 acagttgaca cgcacgattt tgcaaaagaa cgatattttc tatttataat tgatctttta
     2221 tttcctctca tttttcttgc ttttgttttg tatatagcac ctgaagtatt aagcgacaaa
     2281 gtaagccaga ttttgtttaa tgctattggg cagctttatt ttgcattctt ttttataaga
     2341 aacttaataa agcttaaatc atttgaatat ataccctggg gtgtacttat ctatgtactg
     2401 tcgattatca gtgtgtttgc atatttggtg cttaatctga atgcaagctt ttcaaacaac
     2461 gctgaaacta atgaattctt tttaattgta ttaaagatct cattgatgct tctcttttat
     2521 acattttttt atagaagtac tggtaattta acaggttata tatctgataa acgtatttta
     2581 gcgcagaggc ttgttgaaaa cctgatccct tttatttctg tattgtttct tatcgtatct
     2641 gtactctggc tactcgagat tattcatatt tcatcaaaca tattcagggg gctagtgctt
     2701 tgtataatta ttattttagt ctatgcattc cttagggcct actccacagc acagcttgca
     2761 acaaaaattt tcagtcagtc cgagtcatac ctagagcttc ttagtaaatt acagctgctc
     2821 cttaagctgc tgcttgtgta cggattgtat aagatatttc taactttctt caattttgga
     2881 tattttgtac actatctgca gtctctgttt atattgaaaa ccgatcttgt taagatttct
     2941 tttttctctg ccatatcatc agtatttttg tttcttgtcc ttttttctct cataggagtg
     3001 tttaaaaaca tcgcgtttct ttcctacaat aaaagaggaa aagagatcga ggcgggaacg
     3061 acacgggcaa ttatttcaaa cctgggactt ttattggtga ttattatttc actctctcaa
     3121 cttgggctta actggaaagt gcttatcccc tttgcgggtg ctcttggaat cggaatcggt
     3181 tttggtctgc agacaataat gaacaattac atcagcggat ttattttact atttagcaag
     3241 aaactaaaag taggggatgt gatagagata gagggtaatg ccgggagggc aattggcaat
     3301 acacttgaga ccatatacgg cagggtcgag agcattgatg tacttagtgc tgttgtgaag
     3361 actactgaca acatagaaat agttattcct aactcacaat ttattgaagg gcagatagta
     3421 aattactcac tttctgatag tgatataaga ctcaggatcc cgtttggagt atcatacgca
     3481 tccgatccaa accagataaa ggaaatttta ttgaaagtga gcaaggacca tccttctatc
     3541 ctttcgagtc ctgcaccaaa tgtttggttt aaagaaatgg gtgatagcgc acttgttttt
     3601 aatttactct gctggataaa catcaggatg ctttggagaa taaatcctct tataagtgag
     3661 atatatttta agggatggta tgagcttaag gaagcagggg tagaaattcc gtttccgcag
     3721 cgggatttgt ggcttaagaa taagataaag gtagaaatag aaaaatagaa gctgtatatt
     3781 ttatagattg tatttctcat atagcaacct ggataaatga aaaagcttaa gattattata
     3841 agtattgtta taagctactg gcttctgtgc ccacttcatt cttcagcaag cgatgtacta
     3901 aatctcacta aagaacagaa ggcctggata tcctcaaatc caaccataat tgtaggtaac
     3961 gaaattgact ggcctccgtt tgattatgcc gaggatggat tagcaaaggg atattctgtt
     4021 gaccttatgc gacttattgc taaaaaggct gggttaggta tcgagtttgt aaatggttac
     4081 acatggcagc agttaatgca aaaatttaag gcaggcgaga ttgatgtcat gccagctata
     4141 tatttaactt aacaaaggaa ggaatttata aattttagta gtagctatta ttcccagccg
     4201 tctgtaatag tggtaaataa aaatagtaag tatataagtg accttgaaca attatccggt
     4261 aaaaaagttg caggtgttaa tgggttttca ataacaaaaa cattaatgga gaactatccg
     4321 aatatagaaa ttataccggt aacaagtact gtagacggta taaaggctgt ttcggtaggt
     4381 aaagctgaag catttataga tagcattggt tttgtatcat atatattaga aaataatttt
     4441 gtacctaatg tcaaaatcat tacagatgct gatttaaaag aggtatcaaa ccctgcgctt
     4501 cacataggtg tttccaaaaa aaatggaata ttaagagata tcattgatct ggcttttaaa
     4561 tcaataagca gtgaagagga aaaacttctt catgacagat ggcttacttc aagtctaatt
     4621 gaaaaggatg ctattcttaa aaaaagtgaa ctcaatctca ctaaaaaaca gaagaagtgg
     4681 cttaaagaac acccggttat tagactggct cccgatatca actggcctcc atttgaatgg
     4741 gtagatgaaa ataatgagta taagggcctt gcttcggatt acatgagact tattgaggag
     4801 aagcttggaa tagagtttta tgtagagaaa gataaactgt ggccgcaggt agtggaagat
     4861 gtaaaagata agaggcttga tatatttcca tgcgtggcaa gtaatccgca aagggaaaaa
     4921 tatttgaact ttacacgccc atatctatcg tttccaatgg taataacgac tcttgataca
     4981 agcggatata tagacggggt taatagatta aaacatatga gtgtgggtgt cgtggaggga
     5041 tatgctacac atgagtattt tgaagcaaac catccggata ttaatgtcaa aacttataag
     5101 tctgcaaccg atggactgaa ggatttatca aagggtaaaa tagatgcgtt tgtggggaat
     5161 ttggcaacta catcatatct tatacctaaa ataggcctta ctaatcttaa gatctcgggt
     5221 gaaatgccta tccgatacga tttgggggta ggtataagaa aggactggcc ggagttagct
     5281 gctattatgc agcaggcttt tgattctatt accgatgaac aaaaaaacga gattcataac
     5341 aggtggatag gggtacgcta tgaatacggg tttgattaca gtctattttg gaaaatcctt
     5401 ggagtatttg tgttagttct gggtgtttta tatttttata accgtaaact tgcagaagaa
     5461 gtaggcaggc gtaaatttgc cgagcaggag ctaaaacata tgaataagga actgctcgaa
     5521 gccagagatg ttgcccaaag tgccaatatg gcaaaaactg attttttatc gagaatgagt
     5581 catgaactcc gcacaccact cacagctata attggctttg ccgagttatt cagtaaagac
     5641 aacaatctgg atgaagaaca taaaaaagat gcttatatta ttcacagtgc cggccagcat
     5701 ctgcttaata tcattaatga tctgctggat ctttctaaaa tagaatcaga caacattgaa
     5761 atcgaaatga aggacataag tttgaacaag atagtaaacg agtgttttaa cctgataaaa
     5821 ccccttgcag cagaacatga tattcaggta atcaataacg ttactacaaa ttcagctcat
     5881 atgatatatg ccgactatac acgtacaaaa caggtgttaa ttaatctgct ctcaaacgct
     5941 gttaaatata acaagagtgg aggcacaata actattagcc atgaaattgt tgatgatcgg
     6001 atacgtataa aagttataga taccggaaaa ggacttatta aacagcagat agaaaaacta
     6061 tttaacccgt ttgatcgtgc gggggcagaa gatagtcaaa taaagggtac aggtattgga
     6121 cttgtgataa gcaagaggct tatagaaagg atgggaggtt ctataggagt tgagagtgag
     6181 ccggggcagg gttctacatt ctggattgat ctcaatctaa gtcagtctga aacaccatct
     6241 gttattaaag aaaatcctat tgatattgta aaaaaatcta caactaacgg ggcaaaaaca
     6301 attctatata tagaagatat agagcctaac atcaggttgg tgtctcatat actaaaaaaa
     6361 catactaatc ataatcttat aacagcatct agtgctaaaa ttggattaga gatggcgaat
     6421 gtagataatc cagaccttat tcttctagat ataggtcttc ctgaaatgga tggatatgag
     6481 gtcttgaaac atttgatgga taatgaaaaa acccaacaca taccagtagt agcactaagt
     6541 gccaacgcaa tgaaagttaa tgtgcaaaga gccttggatg agggttttaa ggattatctt
     6601 actaaaccca ttgaagtaaa aaatcttctt acatgtataa acaatatata atcataagca
     6661 atgacgaatg taactgttgc atacgggtta taattctaga aattttctta ttaagcaaaa
     6721 aaatacgata ggtgtttata tgaatcttaa taagagggtt ggtataacat ttcaataatg
     6781 tgaccaatga ttttaataat aggttaagcc tgggatggaa aaataatttc acatttaatt
     6841 attattggtg taacctaaaa ttgctgtggg taaatttctc gtattattcc ttattgtata
     6901 tttgtgtatg catttttata aaagatggaa atataagaag gataactcca taggaaagat
     6961 aagatattat gagaatgaac ttgcaaaaaa agattatgat gagggatcta ttattgaaat
     7021 agacagtgat gaagatgatt cccaaccccc taaatatctg cactgagtgt ttttaactta
     7081 agtccccaat ctgcttttca cttccataca gaccaatata catatatgca gatttcatct
     7141 cgggcttttg aaaaagcaca attccaatta tgccgcctga tccaagcagc attgagaagg
     7201 cgcctcctat tataatcagc attcttttta atttcatcca gagggttgtc atttacctta
     7261 acaaaggata attaattata caactttaac ctgacactta ggataaaata aattaggtat
     7321 attgatgtag ctcaatgtct ggtaaagcta ttgtcatcca ggttaaattc tacagagtcc
     7381 tttcgcacta tttcaatttc gtcattgttt tgctctaaga acaagtagtc cttttcaaaa
     7441 agtgagtcag aaaatctcgt aatattttct tcagtagttt taatagttac ttttgataga
     7501 gtgtcattat ctctaatttc cagaggtctt attgtgtaag ggtttttttt gcccgggaat
     7561 tgtttgagga taaaagaatt aagcttggtt acaaactcaa tatgactttc tttgggtatg
     7621 gaaacaataa aatagtatat cttttcattc atatctattt aatgttattt gggcatatag
     7681 agtatggaat ttaactaaat atcaggatat tttgaaactc agtttgcagg aagggtgaaa
     7741 ttgaatttac ttcccgagcc aacctcgctt tgtacactaa ccgttccacc aagtttatgg
     7801 attattctgt aaacaataga aagtcccaga ccatgtcctt tcttacggta ctgatcataa
     7861 ggcataaacc ttgtaaataa tcctttttga tcttcagcgg atattccgtt accgttgtct
     7921 tttatccaaa actcgatgtc gccgtttgaa agccctgttg atccaatttc aattattgga
     7981 ggttggcccc cggattttat tgcattgcta agatagttta cccaaacttc ttcaatccag
     8041 ggtgcatagc caagagctcc tggccacgaa tctggcgttt taagttcagc attaaattcc
     8101 ttgagcataa aatccacgcg ttttttggct tcttcgacta tttctgccat atcgagattc
     8161 tctttttgca catctgtatc acgtacttcg gccagatata acatctcatc tattacactg
     8221 gacattttga atgaatagtc tgttattttt tgcaggctat caccaagctc cttttcagaa
     8281 acaagcggga aatcatcttc caatatcttg gaatagctaa ttataataga aagtaaattt
     8341 ttaagatcgt gtgctactgt atgatcgtag gatgagagct cttcatttct tagtctgagc
     8401 tcagttgttc tttcttctac cttctgttcg agctcagcgg ctgtgtccag aaccgattca
     8461 tatagtcttg cattatctat agcaattgac gcatgtgctg caagtgcagt taaagtatct
     8521 atatcatctt caacgaaaac atcatctcct cttttattta cagcttctaa aacaccaatg
     8581 catttttcat taataatcag tggcactccc agtaagcttt ttgattcgaa gccaaggatt
     8641 ttatcaatct tatcaaaaaa acgtctatta gacttcacat catgcactat ttgaggttta
     8701 cttgttaagt atatctgccc cgcaatagat tcatttaacg ggaccggaac atcggaaata
     8761 agatttaatg cctttatagt tgtagcttta aaacgaagat catcggtatt ctcatctaca
     8821 agaaatattg atgtgcctgc acagtctatt acctcagcgg ctgttgtaac tatttctttt
     8881 aagagcggat cgagtcttag agaagagctt agcttacagc taatttccct tattttctct
     8941 aacttttcta ctctttttgt tagagaagct atttcattat taagagattg agaagtgcta
     9001 ttaatatcat tcataaataa tcaaattata ttatatataa atttacttat tctctctatt
     9061 ctccttcata tagttaattt tatgatcaga atcataaaat ttcaggtgct cttaataagt
     9121 aagactttaa aaccaatctt tctatctaga gattgtatga taaaacaata aatattgcaa
     9181 ttaataatgt atattaataa tttttaagta tctttagagt gaattagaaa ggatttggtt
     9241 ataattaaga ctttggtact gatttctatt caaaaattat tgttttcttt cctcttacca
     9301 atattcggtc ttcaaaatga aaatgcagag cacgtgcaag aaccattctt tcaagatccc
     9361 ttcctttgcg ttttaagtct tctattgaat ctttatgaga aattcgaatg atgtcctgtt
     9421 ctattatagg cccctcatcc aggttttcag tagcaaagtg actggttgcc cctattattt
     9481 tcactcctct ttcaaacgcc tgcttgtatg ggtttcctcc tataaaagcc ggaagaaatg
     9541 agtgatgaat atttatgatc tggtttggat actcatcaat gagcacaggt gataggatct
     9601 gcatatacct tgccattact atagtgtcta tgttgctttc tcttaataga tccagttctt
     9661 tttgttcctg aacctgtttt gattccttag atatttcaaa caaatgatat gggattgaat
     9721 agtgatctgc cagcggagca aggtctggat ggtttgaaat tataaggggt atttcgatat
     9781 cgtattcacc agtgctgtgc ctccaaagaa tttcctgaag acagtggtca tattttgata
     9841 caaatattgc tactctcttc ttcttatcct taaaatttat tttccattgt gcattaaact
     9901 gatctacaat gggcttaaat ttttcgttca gatctgatgg ggaaatagaa ctaccatcca
     9961 tttcccattc aatcctgata gaaaaaaact tttcatctat ttcaacatgt tcaaccagat
    10021 cgagtatgtt tccccctatc tcaaagataa aatgtgagat ttttgaaaca aggcccggtt
    10081 tgtcagggca ggaaaaaagc aaaattgctg agagtttatt attagtcata tattatattg
    10141 agctattatg ttctgaagac atattatata caacaaatga gaaagttgtt acttcacgtt
    10201 tttctctgca cggcttcagg tagattgaat acacggctca tctctttttc ctcaaacata
    10261 tcaggataat taatatattc taaaagcata tcggttgtat caaaacccca aaaaagctgc
    10321 ccgtttataa ttgttgtagg tactccaaaa atacccagat tcactgcttg ttctgtattt
    10381 gtacgcagtt gttcttttac ttgaggctgc tttatcatat cttctggatt ttctactcca
    10441 agctcttttg ctaatttatc ccatgcctcc tcatcttcta aagattgtcc attgacccaa
    10501 atataattaa aaatcttagt gatcgcatca atattattat ccaatgctat actaagcctg
    10561 agagctttta gaggattgaa aggatgggcc gggggcattt tgaaagggat attatttttt
    10621 ctggcaagcc agactatatg acgataagtg agctttcgct tagcaggtat ctcggcgggt
    10681 cctttgtgtt catgatgttt taggaggcct gcaaacagca cgggtttata attgatatta
    10741 atattatccg gtattctatg cagctgagta aactgcaggt agacaaacgg ggatatgaaa
    10801 tcgaaatacc aatcaagctg tgtcattgtt tgttaacctt tattatccag aggtttggat
    10861 gatacaataa tgccattatt atcggcataa agatattcgc ctggattaaa agttacacca
    10921 ccaaatgtta cagagatgtt taattcacct atcccctttt tttctgtttt aagaggtaca
    10981 gtgtttaaag cctttacacc caggtccatt tcagagattg catccacatc tctaatgcag
    11041 ccataaataa taaatccttc ccagccgttt tttaccgcat ctgcagcaat aaggtctcca
    11101 agcagcgctc ttctaagtga tccgccacca tctacaacca taacttttcc attaccatta
    11161 gtacctgcag tttgttttac cactgaatta tcttcatgac acttaatagt aactatctgt
    11221 ccgccgaatg aagatttgcc gccgtagctg ttaaagatcg gctccactac tcttacaaga
    11281 tcagtatatt cgtcacaaag atcgggagtt tggtatttca tagtcaattt tattttactc
    11341 caatttgttt tacttttaat aatctttttt gatagcggct ttgaactaaa tcagtcagca
    11401 ctaatacggt aattacaaaa taaaatgttg tcttgctcat aggagttatt gcctgaccca
    11461 ttatggtcaa atgagctaaa tggccgcctt cagtaagaag cataattccc acaacgaata
    11521 aaatgaatag tcccagcact tcgtacatcc tgtttctttc caaaaattcc gccacactat
    11581 cggaaagcca gatcataagc gccccgccga tgatgatagc tgttgccatt acccagaata
    11641 ccttagtaag agccattgca cttaagatgg agtcgaatga aaatacaagg ttcatagaga
    11701 ctatccagaa tataatcata gaaaccgagc ttttttcctt ctcttctttt ccaagatcct
    11761 caagccctat catatgaaaa atttcctgta tagatgtgta gataataaac actccaccaa
    11821 gaagaactat tatactgtgc agattaaatg ttccttctat aatgcttgta tgaattccaa
    11881 aaagaggatc ctggaaatat tgtataagct ttaataacag aagaagaagc actattctaa
    11941 gaactacagc aattccaatc ccaagcctgc gtaccattgc ctgcttctca gccggagcac
    12001 gttttgattc cagtgagata tataaaaggt tatcaaagcc aagtacagcc tgaagtaaaa
    12061 tcagcagacc aagtgataca atgttgtcga ggttgagtat ttgttccata tataatttaa
    12121 taaactatat actcatttta ttagtgccta tgtctaaaat tttataatac agaacagatt
    12181 atattgcaaa tatttattag ttaagtaatc acaaaaggga ttgtgaaaaa tataaaaggg
    12241 gactaaacct taaaataagg ttgttactca gattcttcta caaaatccag tgctgcggag
    12301 tttaggcagt atctaaggcc ggttggagca gggccgtcgt taaaaacatg accaaggtgc
    12361 tcaccgcact tgcttgaaac aacttcccac cttgtcattc caaaagaggt atcttttata
    12421 agcttgattg aatcttctga aataggctga tagaaacttg gccaccctgt gcctgattca
    12481 tatttatgat ctgaatggaa tacaggctgc ttacatgcag ctgagacata gacacctttt
    12541 ttcttgttgt ttagtaaatc tcctgtaaaa ggtctttcgg tgcctttttc ccagataaca
    12601 tgatattcaa gttttgtaag ttttttcttc cagtcttcct tagtaagtcc ggttctctca
    12661 agtacttcat tttcagacat ctggtctgca ttggaggata tatttattac taaaagtgct
    12721 gtaagaaata ttaataaagt tgctatgatt aatttattca tttttcatac cctaaactat
    12781 tagattagca gataataaaa aggatacata ttatatgatt tatcttgatg gaagtgtgtg
    12841 aacttttcct ttaagttcaa aatgggcgaa aaactgtccg aaaactaagt tacttaattc
    12901 tataagcctg tctatatgtt ttggtgtttg ctcatggata ttttcaggaa ttatttctcc
    12961 atttgtattc ttaatttcta ttttatgtgc aggtacatta agccaccttt ctataagatt
    13021 tgtatcaact tccagatgaa actgaaaccc atatgcattc tcaccgtatt tgaatgcctg
    13081 atttttacat agcttggaag atgcgagatg aactgcaccc ttgggtattt caaatgtatc
    13141 accatgccac tgaaaaattt tttcagtacc tgcaaaatga gaaatcagat cgtccttctt
    13201 tccattagat gttggtgtta cgtcatacca cccgatctcc ggcttcttat ttttattcac
    13261 ttttgcccca agcgcttttg caagaagctg tgcaccgagg cagattccaa gcacaggttt
    13321 tcgttcatct attgccttcc ttataacatc tatttctgtt aataagtttg gatattcatc
    13381 ggtctgatca acattcattg gacctcccag gactatcagg ccgtcataat tgtcgatatt
    13441 aggaattgca tccgggtgtc tttcgaagtt cacatattta atcctgaacc ccgcatttct
    13501 tagaagtgga tctagtgtac caagaatttc gtaggctaca tgcctgaata ctaaaagctt
    13561 actcataatt ccaattcatt ttaccatcaa aatatagaat ttgtgacaga ataataaata
    13621 aaaaatatat aatactttag tttaataata attcctatcc aagcataaat atttagtatt
    13681 aaatacaatt aatactttag tattgctttt tttataaagt gggtttatca tttgttatct
    13741 taatttagtt gtgaggatga tgatatgccg ccaaataatg ataaaggcaa caagggtgga
    13801 aagggtaaaa actcaggaga ttctggggta cccacatctg ttgttgctct aaacaaaatg
    13861 ggttttggac ccaggcccgg tgatatagcc gattttgagg cacttagtaa aagtgagaag
    13921 aaacggcttg ataattatat cgatcaacag actgacccca gctcgataga cgatacagag
    13981 ctagatacaa taataagcga ttatcaatct gcggggtatt ttactaccat caataaaaca
    14041 aggactcaac tctggcagga gcatgtaaaa tcgagtatgg gttctgatca agtaaaacct
    14101 gtaagagaac ttgaactact aactcttata agggctacac acagcaagaa acagcttgta
    14161 gaagtgcttg ctgatttctg gcattcacac ttcagtatat attttggtgg aagcccggaa
    14221 cgttcgatgt ctattcatta tgacagagat gtaataagac cgcatgtact cggaaattac
    14281 agagatatgc tggaagctgt aagccagagc acaagcatgc tctactattt agataactat
    14341 aataacaggg cttcagggcc taatgagaac tggtcgagag aactactcga gcttatggca
    14401 atgggtacgg ataactactt cggatcaatt ccacccgagg atgtaccggg atatccaaat
    14461 tcactcggct atgtagaaaa cgacgtgatt gaggtcacaa ggtgttttac aggctggact
    14521 gttgcaaata atacaagtag aggacaaaca ggggaatttt attatcagga aagctggcat
    14581 gatacggatg ccaagcttgt tcttggtcag cagatacctg cagggcaagg accgcttcag
    14641 gacggactgg atgtgatcaa tattttggca catcatgaaa aagtggcgga gtatatcgta
    14701 tttaagcttt gcagaaggtt tatatcagac agtcctcccg cagatgtagt tgcggcagca
    14761 gcaaacacat ttttaagtaa tgcagacagt ccgcagcaat taaagcttgt tatggaaaca
    14821 attttaaaac acagtgcatt caaatcttca tatggtggca agtttaaaag aggatttgaa
    14881 tttacagcta gttccttaag gactttaggt gctgattttt cggttcttat agatgaatcg
    14941 tggctcaatt cttttataaa tagatacaga ctcattggtc agcatctgtt tcactggaaa
    15001 ccgccaaacg gatggcctga tgttgcaggg gcttggcaga attctaattc atatgtgatg
    15061 aggtggaggc ttgcaaattg gtttatagac agaaagatcg gtgacacact tgcaattgat
    15121 gttatagggc aggctcccca gactgagcag agtgcaacag agatagttga tttctggctg
    15181 gctgagttaa tcgggtacac aggtattgcc gaggcagaca ggacagagat gattaatttt
    15241 atggcagatg gtggtgaccc tgatgtactg ctcgattttc cagggaataa ctcaatcaga
    15301 gacagagtta ggtcattaat tgcccttatt cagatgtcac cagaatttca gatgaaataa
    15361 taaaagcaaa gttgtgggag gatattaaaa atggctgatt tttcaagacg acagttttta
    15421 gttgggtgtt cggcggcaat agctgctatg gctggttcga ggttaacatc ggtagttatg
    15481 ggtgatccgc tagcagagcc tgatcaggaa ataatgctta ctatatttat tagaggtggt
    15541 tgggatgcac tcaatataat gccgccgttt gacggagcag acaggggttt ttatgaagca
    15601 gcaagaagta ctattcaggt acctgctgat tctacttatc aattaggaac taataatttt
    15661 ttctttcacc catcggctgc tagactccat gagctttaca acgcaggtta tttagcggca
    15721 gttcaggcaa ccggtattct taatttaact aataggagcc attttgattc catgagcttt
    15781 atggagcttg gcacaccggg ttcaaaagta ggtacatcgg gctggcttgc aaggcacctt
    15841 gaatctgcaa acatattcgc aggcccggaa gagcaggcct ctcttgcaac aggaaattta
    15901 tctcccactt cccttttaag ctacttagat actcttacta ttgcaaatcc taataacttt
    15961 acccttaata ttgggccgtc agcctggaga acagagcaga gacaggcgct tacttctata
    16021 tatggcggag agagtcaggt tgaggtaacg ggaagtaatg ccatagcatc gcttgatatt
    16081 gtagcaaaca atctgcttgg aagctataca cccgaaccag gagtaaatta tccgggtggt
    16141 ggcttcggaa gagatttaca gaccatagct aggcttatta aagctcaggt tggcctcaga
    16201 tcggccactc ttgattttgg cggatgggac actcataaca accagggacc gggtggcgga
    16261 ggatatttcg caaaccatct acagactttt tctgaaggaa tttatgcgct ttttaatgac
    16321 ttaaatactt ccactactga ttacaggcag aggtttacca tggcaataat gagtgaattt
    16381 ggcaggactt ttaatgaaaa tgattcactt ggaactgatc atggtaaagg aaatgcaatg
    16441 tttgttctag gcggctgtgt aaacggcggc cagttatatg gatcatggcc ggggcttagc
    16501 ggtgagcagc tgcatcaagg cagggaagta gatgtaatga ctgactacag acaggttcta
    16561 agcgagatac ttataagaag atttgcaaat cctaatctca gcactgtatt tccgggttac
    16621 agcggataca ctccacttgg agtagtaatg ggagacgata ttacacctac ttaattagtt
    16681 atattaaaag attattcaaa ataatttatt ttggtagtgt aattttaaac tcagtaaact
    16741 ttccttcctc ggtctcaaag gaaagagtac caccatgctc ctggactata atgtcatagg
    16801 agatcgatag tccaagacct gtgccttcac ctgagggttt ggtagtaaaa aatggtgtga
    16861 ataatttatc ttgtacttct actggaatac catcagcatt atctttaatt cttatctcaa
    16921 cttcattttc gagattattt gagcttattc taacctctgg agagtagtca ctgcccctca
    16981 ttttatactt tttactggtc tcataaaatg cattagtaag aatatttaat agaactctgc
    17041 taatatcctg aggcacaatg cttagttttt ctatcgaagt atcaaagtcc ttattaattg
    17101 agacattaaa agtgtcgtcc tgtgccctca ttccatggta agcaaggttt atatcatcat
    17161 ccaacagagt gttgatatct gtaagctgct tatccccttt tttgtctctt gagtgctgga
    17221 gcattgtgtg cactattctg tctgcacgtt ttccatgctc attgattttc ttagaattac
    17281 tctgcaggtt ttcaatgata tcggttatat ccagataagt ttcttcagaa atattatcct
    17341 tttccttatc caaagcttca tttaattctt cagtaagttc caacgctatt tcagaaaagt
    17401 tattaacaaa attgagaggg ttctttatct catgagcaat accagctgtg agtgtaccaa
    17461 gggatgcaag cttttcctgt gttataagtt ccttctgaat gcttttgatt ttttctaaat
    17521 tttcttcgag ctggccaatt aaattttgtc tttcttcagc aagtcttgca ttgctaattg
    17581 cgtcttctat ttgtgaaacc actattttga acagttcctg ctcaccgctt tcaaatgcat
    17641 atttattttt agaacttaca ctgagcgcac ctataatctc ttctttgtct cttagaggta
    17701 tgcaaagaaa actgtttata ttgagccttt cgctggtctt gccaataacc tcttcttttg
    17761 ttgcatcagg agagtaacga gttacccctg ctgcaatggt tttccacata tagccgttgg
    17821 ggtacggaat gatttttgct ttttctaaca gccagtccgg aaggccgctg tgtgctttga
    17881 gtattaaaca attgtcttca attatataga tgcttattaa atgtaaataa tgtagattct
    17941 cagaaagtga ctgtactgtt ctttcgagta cttcacccag gctgatagac tgatgaacat
    18001 ttgtggaaat agagttaata atagatacaa acctgttttt tctctcaacc tcactaatgt
    18061 tttcctttag caccagttca gttttctttc tcttggttat atcggtgcca taaagatgca
    18121 caaaaccata ttccggtatg ggtctaatgt tgaactgata atatttagaa tttatttcaa
    18181 actctaattg gtctgattta ttttctttaa taagcttttg aaagatgcct tttgtggttc
    18241 cagggcataa ctcaaaccat gataggcctt ctatctcgtc ctttttaaaa agctttttag
    18301 aaccttcatt tgccacaaga acatttccct ttgaatcgag cctaagtacg ggctcggggt
    18361 ttacctgtgc aaatagatag gatttgtcag aatcgacatt attcataaga attagaatat
    18421 attaaaatat gtgaataaag aaaaattaag atatgaaaat acagatatta cttcatatac
    18481 tgtacacata aggcagttat atcatcagac tgtgtggtac ccgttgaata tgaataaacc
    18541 tcttctacag ttttattaac aagatcggta atcgagtctt gtttatggtc atttaagaac
    18601 ttttccagcc gctcgtcagt gaaaaagaag tcatcagagt cggtagcttc tgttactccg
    18661 tcagtgtata gaaaaatctt atcacccggg cttaaattat aagatttact gctgtaaaga
    18721 ttttcttcat tcataaatgc aagcgctacg ccgtttgtat tttcgatgct taagacctct
    18781 cctgaatttt ttataatgta tggcagatta tgtccaccat tacagtaaat tacctctccg
    18841 gtaaatgtat tgagaactcc tgcaaaaact gttacaaaca tactggattc attatccagg
    18901 ctcagtagct tattaacctc tgcaaggcat acatcagggg gaattcctct catagccgtt
    18961 gcctttataa gtgttctgct catggccata aaaagtgctg caggtacgcc ctttccagat
    19021 acatcaccta ttaaaaaata aagatgatat ggatcaatca gaaaaaaatc ataaaagtct
    19081 ccacctacat tttttgcggg atacattttg gcaaatatat caaattcatt cctatccgga
    19141 tatggaggaa aaactctagg aaccattgag gactgtatct gataagcgat attcagctca
    19201 tcctggattt tcgttaattt ttggcggttg tcctcagctt ctttaagacc ggatacttcc
    19261 tgaatagttt tatcaattgt aatttcgaga tcttcaaaat tgatgggttt agtaatgaaa
    19321 tcaaatgcac caaagttaag cgcttttctt atattaccca tatccccgta ggcagacacg
    19381 attaccgatc tcagtgtggg gtattgttct gtgagcttgg aaacaagagt aagaccgtcc
    19441 atctccggca tattaatgtc tgtaagtaca atatctattt ccttgtcttc ttgtattcta
    19501 tttagagcct caaggccgtt gttagcaaat gtgaagttga attcgttgtt cttaaccttt
    19561 ttcctaaact tctgtttaat aagaagctca agatcaggct cgtcatcaac tactaaaata
    19621 tttgcaggaa gtccattctc ggtcatttgg tttgtctggc acataattgt attatcttat
    19681 agtatcaaag tgctaatttt tttgcaaggt tattatcaat tgaagcatta ataaggagac
    19741 taaaatggat gtgtttgaat gtgttagtac actaagctca gtaaggaatt ttattgataa
    19801 acaggtttca gatgaagtaa tcatgaaagt tatggaatca gcaagacttg caccaagcgc
    19861 ccataatgat cagccatggg agtttatttt agtaaaggat aagaaggttt taaaaaagct
    19921 tggggactac tgcataagcg gaaggtttgt ctctcaggca ggatttgccg tggtaattct
    19981 cacagatcaa tcctctaaat ggtttcagat agatactaca agagcagccc aaaatatggc
    20041 tcttacggca tggagccttg atcttggaac gtgctggatt ggcaggcttg ataccggtgg
    20101 aataatgtca ctgctaaata ttcccaacag atggcaggtt ctgaccgtac tgccatttgg
    20161 ctattttaat aaaacttatg cgaacacaac caaattcaga aaatccccac aggaggtttt
    20221 tcatttggat gggtatggga ataaactatc atgataataa acaattgatt aatattggca
    20281 aactttgtat aatttatgtc ataaattaat attttggggc ttaaatatgg ctacattaaa
    20341 tatttcatta acagattcta tgaggaaatg gattgatgaa caggtaaaaa gtggtgaata
    20401 cgcaaacgca agtgattata tgcgtgattt aataaggcat gaccaacgcg agcatgaagt
    20461 aatcaggctt gcacttattg aaggggaacg aagtggaatt agtaatagaa aagtttcaga
    20521 catagttgca gcaactaaag gttctctaaa gaatggctga ctatgagctt tcaggaaagg
    20581 ctgatcagga cctaactgaa atttatattt attcctacaa caattttgga gaatcgaaag
    20641 cggattctta tttcctggga ttagaacaaa gtttaattaa gctagctgac aaacctaatt
    20701 taggaagaaa gatcgattat ataaaacaag gatattttcg atatgaatat gtaagtcact
    20761 caattatcta taaacaaaaa gaagctggaa tcttagtaat gagaattctt cacggaagca
    20821 tggatatgga gagggattta taaatatata ttaagtttaa ttataaaccc taaccacatt
    20881 atagagcgcg tcacgttcta cgggtgtata accagcgttt ttgatcatat ttaccatgaa
    20941 gtcacggctg tgaccaagct ctgatggggc gcctgcatcg tggatgatct tttctctcat
    21001 aattgtaccg tctgcatcac tgcatccgta atgaagtgcc acctgagcgg ctttttctcc
    21061 aagagtgatc cagtatgctt ttatgtgagg gaagttatca aggtaaagcc ttgcaagtgc
    21121 atgaaccttc atatcatatg aagccgggaa aaatttaaca tcttttttta gaccagtatt
    21181 atcaggctga aacaacacag ggataaatgc aaaaaacccc ggggattcat cttcaacgtt
    21241 tctaagtctt tccagatggt ctacaacatg aaatggtttt tcaatatgac cgtaaagaat
    21301 tgtcgcgttt gtaggaattc caaggctgtg tgcagttttg tgtatttctt cccaccttgt
    21361 tgcaatagtc tttggtgcgc agatcttctt tcttatatca gggtgcagta tttctgctcc
    21421 tccacctgta agggcatcta ctcctgcttc ttttaagatt tctaatacct ctgttaagct
    21481 cttgccgctt atttcacaaa accagtctat ttcaactgcg gtaaaggctt ttacatgagt
    21541 ttcggggtaa ctgtctttaa ttgattttac aatttctaaa taatgtttaa attcccaatc
    21601 agggtgaagg cctcctacta tatgcacttc cctgatctct tcatttagca tcgaaaggat
    21661 ctgttcaata gatagctcat aggcatttgc tgattttttt gtgactccaa atgcg
//
LOCUS       DBNGSV010000161        10590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1343979, whole genome shotgun sequence.
ACCESSION   DBNGSV010000161 DBNGSV010000000
VERSION     DBNGSV010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1343979"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC159U_05765"
     CDS             <1..777
                     /locus_tag="AC159U_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK family nuclease"
                     /protein_id="HIR3336598.1"
                     /translation="KSKNAHKYINIYDLLTSETISPEYVKKMILNQALKRNIIVLNLG
                     GEILFNNIPGTLHIRIKSSETTKSSKTNKANYKKLIHSIYDVSRLETKYYDLQIKIDN
                     NDTDFALELISKALEIKGILSKAGITWNAINKLKKSINHSSISTTKNSYHHKVPDFAH
                     PSEKEFAKVLDYYRINWEYEPRSFILQTYDSGSIKEEFTPDFYLTDLDLYIELTTMKQ
                     KLVTKKNRKVRKLKELYPDVNIKIFYGKDYQKLLYRFGIK"
     gene            1093..1629
                     /gene="hpt"
                     /locus_tag="AC159U_05770"
     CDS             1093..1629
                     /gene="hpt"
                     /locus_tag="AC159U_05770"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR3336599.1"
                     /translation="MIDDIKEILISEAELKDKTAQLGSKISQDYKGLNPVFITVLRGS
                     IIFVCDLIREVKIPISLDFLSVSSYSGQSHTGVVRIIKDLDENIENKHVILIEDIIDT
                     GLTLNYIVSMLQERKPTSIKVCALLDKKVRRIIDIPINYKGFEIPDEFVVGYGMDYNQ
                     KYRNLPFIGVLKEEVLAK"
     gene            1859..2389
                     /locus_tag="AC159U_05775"
     CDS             1859..2389
                     /locus_tag="AC159U_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336600.1"
                     /translation="MIKGSTISKAYSKATKDLELISVSQDKRSFYKEIIDNITETTER
                     GTVLIHLLDEKQTQATLVAECNIFSDDLKRFITIDNTEHTIWSPIKENRIKLFRNLVS
                     NEKVGEKIKSLGKSSALGVPITNEDGKSIGCIWVLDTNDSVTTPQYIQIRELLLESAK
                     KIAWKEENDKNTSGLH"
     gene            complement(2506..3657)
                     /locus_tag="AC159U_05780"
     CDS             complement(2506..3657)
                     /locus_tag="AC159U_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013636.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336601.1"
                     /translation="MSLDSPDGTNVVLFEDVGGSSDNFGTSCGDQTTTFPDFVLADGA
                     PAIPGAPGVAGTFSSQGNLSDYFLESSIGTWTLNIDDDFTIDTGTLECWCLDIYSTGE
                     GCSPGFWGNRALKDNSDVFAGCPSIDPDITMENLFDMCNLDNCLPNNQIEFADADEIL
                     HPRGRTNRKDCDGKAGAAAQLLRQATAALLNVCTNQVNYPLSAAEIEAEVCSLLTNID
                     GGGGTPKEDITAFAEELAANNSQECNVDADAPLEECSNPADVTIENCTNVDTFGLHGL
                     FPCGEGLCPPDPFFGLPAICFPDADGGSTGHCSQDFLCESVSPCPNGQSDCGVDELCI
                     QSCCEFGNGGFDVCVPLAISCPAPPGMMSSPNTLELFINSGPTSGGFIE"
     gene            complement(3667..3915)
                     /locus_tag="AC159U_05785"
     CDS             complement(3667..3915)
                     /locus_tag="AC159U_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336602.1"
                     /translation="MNKIIVRSIALMVLSLLILKTDAMAGDVPPPNGVLFETYCSTNT
                     PEAIPDSPNPGIGNDLDIASGPTITDVNVRLYITHTWD"
     regulatory      complement(4017..4103)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4291..5688)
                     /locus_tag="AC159U_05790"
     CDS             complement(4291..5688)
                     /locus_tag="AC159U_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3336603.1"
                     /translation="MAVKTLKVLTAYDEKVIDELPLATENDANKMLNAARKLADDPSK
                     RIPIPERIEILERAQQILKDRYDDIVGTACKEGGKPFIDTQVEITRSINGFGVTKEEI
                     ANMRGTEIPMNLNAASLNRMAYTFREPIGVVIAVCAFNHPFNLAVHQVLPAIAVGCPV
                     IIKPASTTPLSSINLVNILYEAGLPKGWAQVITCDNKVSEQLVTDPRVDFLTFIGSGR
                     VGWYLKSKVAPGVRCELEHGGAAPVIVEPDADLDDALPLMAKGGLYHAGQVCVSVQRV
                     FAHSSVTKKVANGLARLAKKMNVGDPLDPKTEVGPLILPREVDRVHDWVKEATRRGGK
                     LLCGGKKIGRTCYEPTVILDPPQKCNLSQLEIFGPVIAVYTYKERDEAIALANSLPLS
                     FQAAIFTKNIDTALDSIKRLNARAVMVNDHTAFRVDWMPFGGNKESGLGMGGIPYSMH
                     EMTQEKMMVIRSSVL"
     gene            complement(5729..7372)
                     /locus_tag="AC159U_05795"
     CDS             complement(5729..7372)
                     /locus_tag="AC159U_05795"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR3336604.1"
                     /translation="MKASDLFVKALEKEGVKYIFGIPGEENLDFLNSLRNSKIKLILT
                     RHEQGAGFMAATHGRLTGKVGVCLATLGPGATNFVTAAAYAQLGAMPMMMITGQKPIK
                     KSKQGQFQIVDVVNMMRPLTKYTKQIVDANNIPSFVRESIRVAEEERPGAVHLELPED
                     VAAENGEERYFEISNVRRPDANKTVIDKAVELISKAKKPLLLIGAAANRNRTCRALRK
                     FVDNTNIYFFDTQMGKGVVDERSPLFLGTAALSDHDYLHCAIDQADLIINVGHDVIEK
                     PPFFMNGHVKVIHINNFSAIVDDVYYPQLEVVGDIASSVERIAKGIKSKKVSRPRIFQ
                     NIKKSLEEHIFDKSNDKGFPIIPQKVVADIRKAMPDDGIITLDNGVYKIWFARNYYAY
                     KPNTILLDNALATMGAGLPSAIGAKIVEPKKKVLAICGDGGFMMNSQEIETAVRLKLD
                     LVVVILRDDAYGMIKWKQSGMGFKNWGLDYGNPDFVKYAESYGANGHRIGSNPELLSK
                     LKECINSKGVHLIEVPVDYSENEKVLIKELNNLTCELVY"
     gene            complement(7463..7912)
                     /locus_tag="AC159U_05800"
     CDS             complement(7463..7912)
                     /locus_tag="AC159U_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3336605.1"
                     /translation="MLRFLFHTNGDFPTFFARVFLGAVILPHGLQKLLGMFGGNGFDA
                     TVKFFVEGGLPEIVAILIIVGESVGAVCLIIGFMGRICAIGIITIMTGAIVTVHAKYG
                     FFMNWFGTQEGQGYEYHLLAIGLGLAVLVGGSGKFSIDRFITNKLVF"
     gene            8149..9177
                     /locus_tag="AC159U_05805"
     CDS             8149..9177
                     /locus_tag="AC159U_05805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3336606.1"
                     /translation="MRIDLLEQLCQAVALPGAEEKLQKIIVKHLKNYTSDITIDCMGN
                     IIAHIKGKGPKVVLDAHMDEVGFMVHYIDPNGFLKLTPLGGMDPRIFYAQRVMVHGKK
                     DLIGVVASIPPHISKNGNNQEVPEVEDCVVDLGLSAEKVHKLVKVGDLVSFCYSFEKT
                     EDAVVAKALDDRVGLFVFLEALKKAKKINCDLYVTATVQEEVGLRGARLITPVVEPDY
                     VVALEGTVSMNIPGIPEDKMLAKALEGPEIRLSDRFLVADRKFSFFIMELAEKNKIPY
                     QVTVKKAGSTNATAMQVTGKGSKAAVVSVPTRYLHSPSSLAFKDDISNTIQLIALLLE
                     NINKFKNN"
     gene            9193..10002
                     /locus_tag="AC159U_05810"
     CDS             9193..10002
                     /locus_tag="AC159U_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336607.1"
                     /translation="MNTNLFRSFIIFTALIILSAPFTYASDTENEKTSVQQVKINTLQ
                     HAQNIFFEYHNDESSFSKAMDILNEELAINPNNCDALVLLSRIWLTYGHYIQDNSSEE
                     KWNRFKTGSEIAKKAIKADSSNPDAYFYYVANEASLAKSKGVFGSFFLVKKIKKNIAK
                     TLELNPNHTETIAMKGALLNSIPGIMGGDVKEAEKLIRQALVLDPHLTSTKIFLAKNL
                     FKQEQYEEAKAVLNEVLEETNPTVLADWHLNKRSALMWLKKINEQQQSKKT"
BASE COUNT         3398 a         2170 c         1795 g         3227 t
ORIGIN      
        1 aaaagcaaaa atgcacataa atatattaac atttatgatc tattaacttc agaaacgatc
       61 tcgcctgaat acgttaagaa gatgatacta aaccaggcgc ttaaaagaaa tattattgtt
      121 ttaaatcttg gcggagaaat acttttcaat aatattcctg gaaccttaca cattagaata
      181 aaatcctctg aaactacaaa gagctcaaaa acaaataagg caaactataa aaaattaatc
      241 cattccatat atgatgtaag cagattagag acaaaatact atgacttaca gattaagatt
      301 gataacaatg acactgattt tgctcttgaa ttaatatcta aagccttaga gatcaagggg
      361 attttatcta aagcaggtat tacatggaat gcgatcaata aacttaaaaa gagtataaat
      421 catagtagta tatctactac taagaatagc tatcaccaca aagtacccga ctttgcccat
      481 ccaagtgaaa aagaatttgc aaaggtactg gattattaca gaataaattg ggaatatgaa
      541 cccagaagct ttattttgca gacgtatgac agcggtagta taaaagaaga attcacccct
      601 gatttttatc ttactgacct tgatctttat attgagctta caacaatgaa acaaaagctt
      661 gttacaaaga aaaaccgtaa ggttagaaag ctaaaagagc tgtaccctga tgttaatata
      721 aagatttttt acgggaagga ctaccagaaa ttactttata gatttggtat aaaataaaaa
      781 aacgactcca agtgacctta gcttgtattt gaaaactgag ccctggctcc gcgtccgctt
      841 tgccgttctc tctttcgaga gatttttgac cgcttttaac ggtctaacct actggttatt
      901 tcaggctttt gttttctgat taaccttagc ctcttggaat cgcttattta tattatagct
      961 ctttttataa actaaagtca ataggttttt ttatctggtc ataaattatt ttttttagcc
     1021 ttattgagtt aattcttata taatcttttt ctaattcaat tactttaatt aaatcaaaac
     1081 gaggcaaacg gattgattga tgatataaaa gaaatattaa taagtgaggc tgaattaaaa
     1141 gataaaacag ctcaactagg cagcaaaatt tcacaagatt ataaaggcct aaatccggta
     1201 ttcataacag ttctccgtgg gagcattatc tttgtatgtg atttgataag agaggttaaa
     1261 attccgattt ccctcgattt cctttcggtt tcaagctatt cgggacagag tcatacgggt
     1321 gttgtacgaa taataaaaga tctggatgaa aatatagaaa ataaacatgt cattttgatt
     1381 gaagacataa tcgacaccgg gcttaccttg aattatatag taagcatgct acaagaaaga
     1441 aaacccacca gcattaaggt ctgtgcatta ttggacaaaa aagtacgcag gataatagat
     1501 atacctatca attataaggg ttttgagata cccgatgagt ttgtagtggg ctatggaatg
     1561 gactataacc agaaatacag aaacctgccg tttatagggg tattaaaaga agaagtactt
     1621 gccaaataaa ccggtccttt attacatact ctaattgcat aaatcatatc cagaattata
     1681 aattttctat ttttctacaa acacccttta cttttttcta aaatccttat aattaacgct
     1741 cttttattga aaagttagcg tggttattaa atttaagaat taattcttat ttaataattt
     1801 aagcagctta gataataaaa ttaataaata tatttaaatt aacattttgg aggatttaat
     1861 gattaaagga agcacaattt caaaagccta cagcaaggca acaaaagatt tagagcttat
     1921 ctctgtatct caggacaaaa gaagttttta taaagagatc attgacaata ttacagaaac
     1981 aaccgaaagg ggtactgtac ttattcatct tttagacgaa aagcaaacac aagctacgct
     2041 tgttgctgaa tgcaacatct tttcagatga tttaaaaaga ttcataacaa tagataacac
     2101 tgagcacaca atctggagtc ctataaaaga aaacaggatt aaattattca gaaatcttgt
     2161 atcaaatgaa aaggttggtg aaaaaattaa gtcacttggc aaatcatctg ctcttggggt
     2221 tcctatcaca aatgaagatg gtaaatctat tggatgtata tgggttcttg atactaatga
     2281 ttcagttacc accccacaat atatacaaat aagagaattg cttctcgaat ccgcaaagaa
     2341 aattgcctgg aaagaagaga atgacaaaaa cacttcaggg cttcactaat tcatttcaaa
     2401 tatccgacaa attttatctt gttacctaac aaataaaatc tacaaaaaaa aggccggtat
     2461 tttactcccg gccctatatt ttttaaaaca attaaattag taatattatt ctataaaacc
     2521 accgcttgta ggaccactat taatgaataa ctccaaagtg tttggtgaag acatcatacc
     2581 gggtggtgct ggacatgaaa ttgcaagcgg aacacataca tcaaatccgc cgttaccaaa
     2641 ttcacaacag gactgaatgc agagctcatc tactccacag tcagattgtc cattagggca
     2701 aggactaact gattcacaca ggaagtcctg agaacagtgt cccgtacttc ctccatcagc
     2761 gtctggaaaa catatagcag gcaatccaaa aaatggatcc ggtgggcata agccctctcc
     2821 acatggaaac agtccatgta gaccgaaggt atccacattt gtacaatttt ctattgttac
     2881 atcagcagga ttgctgcatt cttccaaagg agcatctgca tcaacattac attcctggct
     2941 gttatttgcg gcaagctcct ctgcaaatgc tgttatatcc tcttttggtg taccgccgcc
     3001 accgtctata ttggtaagta gcgagcatac ttctgcttct atttcagccg cagaaagcgg
     3061 ataatttacc tggttcgtac acacatttag aagagctgca gtggcctgtc tcagcagctg
     3121 agccgccgcc cctgccttcc cgtcacaatc ttttctgttg gtacgacccc ttggatgaag
     3181 aatttcatcc gcatcggcaa actctatttg attattaggc agacaattat caagattaca
     3241 catatcaaat aagttttcca ttgtaatatc aggatctatg ctcggacagc cggcaaatac
     3301 atcagaatta tccttaagtg ctctgttccc ccagaacccc ggtgaacagc cctcaccggt
     3361 tgaataaata tccaggcacc agcattcgag agttccagta tcaatagtaa aatcatcatc
     3421 tatattcaga gtccaggttc caattgagct ttctaaaaaa taatcagaca gatttccctg
     3481 agaagaaaaa gtacctgcaa cacccggagc tccaggaata gcaggtgctc cgtcagcaag
     3541 tacaaagtca ggaaatgtag tagtctgatc accgcatgaa gtgccaaaat tgtcacttga
     3601 tcctcctaca tcttcaaaca atactacatt tgtgccgtcc ggtgaatcca gactcacgtc
     3661 taaatcctaa tcccatgtgt gggttatata cagcctaaca tttacatccg taattgtagg
     3721 accagaagca atatccagat catttccaat tccaggattt ggactatctg gaatagcttc
     3781 aggtgtatta gtactacaat aagtttcaaa aagcacgccg tttggcggtg gtacatcacc
     3841 tgccatggca tctgtcttca gtataagaag acttagaacc attaatgcta ttgatctaac
     3901 aatgatttta ttcatcttta tccccctact tattttgaat ttaacaaaat agcaagtgga
     3961 aattggaagg aactttacga tacatataat aataacttag ctttataata gactgtatcg
     4021 gcagccggac taccatggaa tatttttcca acaggctcct agctttgcgt cccatcattt
     4081 ctaatggttt gccatttcgt tatagtatta taatactata gtatggcata caaagtcaag
     4141 gcaaccatat cggtatttgt agcaaattaa tatttttaat cggatttcag gtatttaagc
     4201 aaaaatgtct ctgatattac tcacagccta tatactttta taaaacagat ttcattacat
     4261 tgaatattgt attatataac aaattatcaa tcaaagcacc gatgatctga taaccatcat
     4321 tttttcctga gtcatctcgt gcatggaata aggtattccg cccattccga gccctgattc
     4381 tttgttgcca ccaaaaggca tccagtcaac acgaaacgct gtgtggtcgt ttaccatcac
     4441 agcccttgca ttgagtctct taatagaatc cagagcagta tctatgttct tggtaaatat
     4501 ggcagcctga aatgacagag gcagactgtt agctagagca atggcttcgt ctctttcctt
     4561 gtaggtatag actgctatga caggaccaaa tatttcaagc tgtgaaagat tacatttttg
     4621 tggagggtct agtattactg tcggctcata gcatgtacgt cctattttct tgccaccgca
     4681 taagagtttg ccgcctcttc tggtcgcctc ttttacccag tcatgaactc tgtctacttc
     4741 tcttggtagt ataagcgggc caacctctgt ctttggatca agaggatctc ctacattcat
     4801 tttctttgca agccttgcaa gcccgtttgc tactttcttt gtaactgaac tgtgtgcaaa
     4861 taccctctgt acagatacac atacctgtcc tgcatgatac agcccgccct ttgccatgag
     4921 aggaagtgca tcatctagat ctgcatcagg ctctactatc acaggagcgg caccaccatg
     4981 ctcaagctca catctcacac ccggcgctac ttttgatttc aaataccagc ctaccctgcc
     5041 tgatcctata aaagtcaaaa aatctactct tgggtcagta acgagctgtt cagacacttt
     5101 attatcacaa gttatcacct gtgcccagcc ctttggcaga ccggcttcat atagaatatt
     5161 taccagatta attgatgaca gaggtgtagt acttgccggc ttaattatca caggacaacc
     5221 gaccgcaata gcaggcagca cctgatgaac ggctagattg aacggatgat taaaagcgca
     5281 tacagctata acgacaccaa ttggttctct gaacgtataa gccatcctgt ttagagaggc
     5341 ggcattaagg ttcatgggaa tctctgttcc cctcatattg gctatttctt cttttgtaac
     5401 tccaaagccg tttatagaac gagtgatttc aacctgggta tctataaatg gcttaccgcc
     5461 ttctttgcaa gctgttccta ctatgtcgtc atatctatct tttaatattt gctgagccct
     5521 ttcaagtatc tcaattcgct ccggtatagg aattctcttt gaaggatcat cagcaagctt
     5581 ccttgcagca tttagcattt tgttagcatc attttctgta gctaatggaa gttcatctat
     5641 aactttttcg tcatatgcgg ttaatacctt cagcgttttt acagccatct gttattcccc
     5701 ttatttaaat tatagtaata tacaaacact aataaaccag ctcacatgtt aagttgttaa
     5761 gctctttaat taacaccttt tcattttcag aataatccac aggtacttca atcaaatgaa
     5821 caccttttga atttatgcat tcttttaatt ttgatagaag ctcaggatta cttccaatcc
     5881 tatgtccatt cgctccatag ctctcggcat atttaacaaa atcagggttt ccataatcta
     5941 gcccccagtt tttaaaaccc ataccggact gcttccattt tatcattcca tacgcatcat
     6001 ctctaagaat cacgactacc agatcaagtt ttaatctaac tgcggtttct atttcctgag
     6061 agttcatcat aaacccgccg tctccacata ttgcgagaac cttcttcttg ggttctacaa
     6121 tcttggctcc gatagcagac ggcagtcctg cccccatggt tgcaagtgca ttatcaagta
     6181 atattgtgtt aggtttataa gcataatagt tccttgcaaa ccatatctta tatactccgt
     6241 tatcaagagt aataatgccg tcatcaggca tcgcctttct aatatctgct acgaccttct
     6301 gagggattat gggaaacccc ttgtcattgc tcttatcaaa aatatgttct tctaatgatt
     6361 ttttaatatt ctgaaaaatc cttggtctcg agactttttt actttttata ccctttgcaa
     6421 ttctttcaac agatgatgca atgtcaccaa ccacttcaag ctgtggatag tacacatcat
     6481 ctactatagc gctaaagtta tttatatgta ttaccttaac atgaccgttc ataaaaaacg
     6541 gaggcttttc aatcacatcg tgacctacgt ttattatcaa gtcagcctga tcaatagcac
     6601 agtgcaggta gtcatgatcc gatagagcag ctgtacccaa gaacagcgga cttctctcat
     6661 ctacaacccc ctttcccatc tgggtatcaa aaaaataaat attcgtatta tcaacaaatt
     6721 ttctaagcgc cctgcatgtt ctgtttctgt ttgcagctgc tcctattaaa agaagcggct
     6781 tcttagcctt tgaaataagt tctacagcct tatctattac tgtcttattg gcatcgggtc
     6841 ttcttacatt agaaatctca aaatatctct cttcaccatt ttctgcagca acgtcctcgg
     6901 gaagctcaag atgtacagca cccggtcttt cttcttctgc tacccttatg gattctctta
     6961 caaaacttgg gatattgtta gcgtctacaa tctgttttgt atattttgta agcggacgca
     7021 tcatgtttac aacatcgaca atctgaaact ggccctgttt actttttttg attggcttct
     7081 gcccggttat catcatcata ggcattgcgc caagctgagc atatgctgct gcagtaacaa
     7141 aattagttgc acctggtcca agtgtagcaa ggcacacccc tacttttccc gtaagccttc
     7201 catgtgttgc cgccataaat cctgcaccct gttcatggcg tgtaagtatg agtttaatct
     7261 ttgaatttct aagggagttt aagaagtcga gattttcttc acccggaatt ccgaaaatgt
     7321 atttaacacc ttctttttca agagctttta cgaacaggtc agaagctttc attttattat
     7381 cctcactcct cttacaagta ttttaatacc aatattatat tgttagaggt aactccgggt
     7441 gatatggatt ctgtaaatct tattaaaata ccagcttatt tgtaatgaat ctatcaatag
     7501 aaaatttgcc tgatcctcca acaagaacag caagtccaag gcctatagca agaaggtgat
     7561 attcataacc ctgcccctcc tgcgtgccaa accagttcat aaaaaaacca tattttgcat
     7621 gtactgttac gattgcgcct gtcataattg taattattcc aattgcgcag atccttccca
     7681 taaagcctat aataagacat accgcaccca cggattctcc tactataatc aggattgcta
     7741 ctatctcggg aagacctccc tcaacaaaga atttcaccgt agcatcaaaa ccattaccgc
     7801 caaacatccc cagtagtttt tgaagcccgt gcggtagaat aactgcgcct agaaagactc
     7861 ttgcaaaaaa tgttggaaaa tctccgtttg tatggaataa aaacctaagc attaaactgt
     7921 aatcccctca ttctcttaag acgcattaac agtatttata tagaattatt tatataataa
     7981 tattaatctg atattgatta tagcactaag tctataatga acctatacct tttatttaaa
     8041 acttaaaaat aaattccaag ttaatacact gttattattg ataaatatac cttcaatttt
     8101 aatcttgatt ggaaaccgta gtataaaacg gtataatctc acaagtatat gagaattgat
     8161 ctactcgaac agctctgcca agcagttgct cttcccgggg cagaggaaaa actccagaaa
     8221 attatagtta aacaccttaa aaactatact tccgatataa ccatagactg tatgggtaat
     8281 attatcgctc atataaaagg caaaggaccc aaggttgttt tagacgctca catggatgaa
     8341 gtcgggttta tggttcacta catagatcca aatggctttc tcaaacttac cccgcttggt
     8401 ggaatggatc cccgtatttt ctatgcacag agagtaatgg tgcatggaaa aaaagacctt
     8461 atcggtgtcg tagcctccat accacctcat ataagcaaga atggtaacaa tcaagaagta
     8521 ccggaggttg aggactgtgt agttgatctt ggattatctg ccgaaaaagt acataaactt
     8581 gtaaaagtgg gtgatctggt ctcattttgc tactcttttg agaagactga agatgctgta
     8641 gttgcaaagg cattagacga ccgggttgga ttattcgttt tcctagaagc actaaagaag
     8701 gcaaagaaaa taaattgtga cctttatgta actgccacag ttcaagaaga agtaggctta
     8761 aggggagcaa gacttataac ccctgttgta gagcccgatt acgttgttgc tctcgagggt
     8821 actgtatcta tgaacatacc gggaatacca gaggacaaaa tgcttgcaaa agctctggaa
     8881 ggacctgaaa taaggctatc agacaggttt ttggtagctg acaggaaatt tagctttttt
     8941 attatggagc ttgctgaaaa gaacaagatt ccttatcagg ttactgttaa aaaggccgga
     9001 agtactaatg ctacagccat gcaagtcacc ggaaagggaa gcaaagccgc cgttgtttca
     9061 gttcccacaa ggtatctgca cagccccagt agtcttgcat ttaaagatga tataagtaat
     9121 actatacagt taatagcact tttattagag aatattaata agtttaaaaa taattaaggc
     9181 aggatgtgaa aaatgaatac taacttattc agatcattta taatttttac agcattaata
     9241 attttatctg ctccatttac ttacgcttct gacactgaga atgaaaaaac atccgttcag
     9301 caggtcaaaa ttaatacttt acagcacgcc cagaatatat tctttgagta tcacaatgac
     9361 gaatccagtt tcagtaaagc aatggatatc ttgaatgaag aattagcaat taatccaaat
     9421 aactgcgatg cgcttgttct actttcaaga atctggctta catacggtca ttatatacag
     9481 gacaatagtt cagaagaaaa atggaacagg tttaaaacag gctcagaaat tgctaaaaaa
     9541 gcaattaaag cagattcttc caatcctgat gcttattttt attatgttgc aaacgaggcg
     9601 tcccttgcaa aatcaaaagg ggtattcggc tcatttttcc tggttaaaaa aattaagaaa
     9661 aacattgcca agacactgga gcttaatcca aaccacactg agacgattgc aatgaaagga
     9721 gcgcttctaa actctatccc cggaatcatg ggtggagatg taaaagaggc agaaaaactt
     9781 ataagacagg ctcttgtgct tgaccctcac ctaacaagta caaagatttt ccttgccaag
     9841 aacctattca aacaagagca gtacgaagag gcaaaggctg tattaaatga agtattggaa
     9901 gaaacaaacc ccacagtgct tgccgactgg cacctgaaca aaagatcggc tcttatgtgg
     9961 cttaagaaga ttaacgaaca acagcaaagc aaaaagactt aagatatttc ttttccataa
    10021 atcaacaaac ctgaatttct ccatataaca cttatcccca taacacatat aaccggccaa
    10081 agtgtgtaat ccgttcaaaa tatgtgaata tgccaaattg ccaccttttt atcctatacc
    10141 cacaaaacct ggcattatgg caatattagt aaaatttcca acttcggatc aaaagatcaa
    10201 cgcccatccc ttaacccctg atacttgatc ttttgatatt aactcattaa taatcctgga
    10261 atgcagtcca acatttgttt attatcaaag tttaatctct taatctccta gtctatatcc
    10321 catacctaaa accaagagac taagagactt acttttaata atttattaat ttcggcaggt
    10381 tatgttctac aggctcatag gctcagtggt tttaccctac ccctcagcct gctgagtttt
    10441 tgagctttgg aatggatatc tatataattt ttggagctcg catagaacag agctataagt
    10501 aatgcagaat ctcctttatt ttattaacta aatctacatt tctgttatta aatctaaatt
    10561 ccatctcttt tagatacaaa acaaagtgtt
//
LOCUS       DBNGSV010000162        11108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1067769, whole genome shotgun sequence.
ACCESSION   DBNGSV010000162 DBNGSV010000000
VERSION     DBNGSV010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1067769"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1415
                     /locus_tag="AC159U_05815"
     CDS             204..1415
                     /locus_tag="AC159U_05815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3336608.1"
                     /translation="MASTIPGIKDAREILKKNKIVIAGVGETEQGKIPNKSSFHFLSE
                     ASKLAIEDAGIKKTDVDGLVTAFSLVEHTFMHCTTFADYFGLNPKFFSSIAIGGSTAV
                     WMAAEAAMAIATGQAEAVLCVRGDNTLSGISSSGMVALIREMCHAEFEYPYGLTTPGG
                     YALAAQRYLHEYGATSEHLAAIAVTMRKHAALKENAMNKEPLTIDDVLSSRVIAEPLH
                     KYDCSIISDGGAAFIVTTEEKAKQLGIEKPLAYLWGMGQGYSHQYLTTLQDLDQIYNA
                     VERSGKKAYDTAGIGPKDINLAYLYDCFTITVALEMEGLGFVGKGEAGPFALEGRMEI
                     GKDMPVNTNGGLLSQAHLGGMHHVIEATLQLRGEAGPRQVDNAEVALVHGNGGIVSAH
                     STILLGNQALS"
     gene            1437..1856
                     /locus_tag="AC159U_05820"
     CDS             1437..1856
                     /locus_tag="AC159U_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3336609.1"
                     /translation="MSEENKYNKPLPEFRPETKPFWDACKNHELMLPKSKETNKFFFY
                     PRAFSPGDDMAEVEWSKASGKAKVWTFSIHHMGPTKAYKGDGPYVVALVETEEGVKMM
                     TNIVDCDPNDVHMGMDVEVVFDDVTDEVTLPKFKPAA"
     gene            1882..2334
                     /locus_tag="AC159U_05825"
     CDS             1882..2334
                     /locus_tag="AC159U_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3336610.1"
                     /translation="MAQDRILYEDLEVDAEHKSSGRTVTEADVVNFAGLSADYNNMHI
                     DEEFAKKTVFKTRVAHGMLVTSIATGLWFTMPRLATVAFMGLEDWRFSGAVFPGDTIH
                     ITRKLVEKREHKRPNMGFFIFEVNVHNQKDEVVQKGKWVILVNRKESS"
     gene            2573..3097
                     /locus_tag="AC159U_05830"
     CDS             2573..3097
                     /locus_tag="AC159U_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HIR3336611.1"
                     /translation="MVPVEALLGLVLLLVGFVFFGNGMTLLGKTGAKEIGVLNLGVGI
                     IISIAAWQLHGLGLTAATALVSVFALIYFMVAGIFIHGHDGKGLGWYCLFATIVFLWY
                     ASHFSSLGLTYMAGFSVAWAVLVFFAWTALALGKGTATLVAYLFLIESIVTLLIPGML
                     LLTEKWNPLGGVPG"
     gene            complement(3218..3430)
                     /locus_tag="AC159U_05835"
     CDS             complement(3218..3430)
                     /locus_tag="AC159U_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336612.1"
                     /translation="MKVVSPAGDFEITIEDSSVEQDSIVLKGQMGVWDSKIYLKPSDL
                     FIFASMLIRPAIIVFLLKYPFKQIFK"
     gene            complement(3691..4500)
                     /locus_tag="AC159U_05840"
     CDS             complement(3691..4500)
                     /locus_tag="AC159U_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HIR3336613.1"
                     /translation="MAYCIRADINAMKAIVPEPIEVQDDVIEFWYVIHRNTMLGPYME
                     FGVTFSATVDDGSGHIYKAGYYPYMYLSNDSPIFAGKEPFGFPKKAAYIAGFEHGTNN
                     NYFNHLMERRGYILHTANGRYSDKPLSVKPTFYGKTDWGRMNYRITTRPDVSNSIHEV
                     TYLPSELPPGFGTGHRFQLDPQSIRTATGDDIRSWYMSGTPFDFMGGQIPATEIVGLI
                     SFTFNLIIPPAATIWTRTVERGEADFGQILYSKPFEYTMRHRFLLPQYSQA"
     gene            complement(4720..5697)
                     /locus_tag="AC159U_05845"
     CDS             complement(4720..5697)
                     /locus_tag="AC159U_05845"
                     /inference="COORDINATES: protein motif:HMM:NF018066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HIR3336614.1"
                     /translation="MPSHLNVPTKYWELTKEVPPPPIERDIKSWIIANPSDPLWDIDV
                     LPLTYTDSRWSAFVVRVDEPTRRRLVPDPPLDLWDGENADLVEYWYVDHKNTMLGPYL
                     EFGVTVSTSYKDPKGNVWRAGYYPYMYLTGDAPVDAGRVLGFPKKMSYIRCTVHGGEK
                     GTDFFGFAMSRNGYLLHSATGQFDDKQVTPPYFYGKTDWGKFNMKVITRPDVSSSEWQ
                     LTYLPSELPGAMKEMIQGHRFKIKPEHTRTASGDAINWFAQATPFDNMGAEIKIQEVT
                     GLISFVFDLVIPPAVVLWTETYERPASYRGYATPYKYGLRQQFPISQGS"
     gene            complement(5907..6686)
                     /locus_tag="AC159U_05850"
     CDS             complement(5907..6686)
                     /locus_tag="AC159U_05850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3336615.1"
                     /translation="MDRNEKLARDFLDLIQNCKHAVALTGAGISTESGIPDYRSPDTG
                     LWENTDMSVVSLDGFLADPSKYYKYSLKLYPVRSAAKPNPAHYLLASLEKLGFLKGVI
                     TQNVDGLHYKAGSETVHELHGSLRQTVCLGCNLLFPMEEIMQRVVSGENPPYCQECGA
                     VLKPNAIFFGEALPQIPWKESVKLCENADLFIVIGSSLQVSPANILPDMTIKKGAKLV
                     ICNIQATPFDTDATLIVREKIGGFASLVASLLESNITDTES"
     gene            6916..7830
                     /locus_tag="AC159U_05855"
     CDS             6916..7830
                     /locus_tag="AC159U_05855"
                     /inference="COORDINATES: protein motif:HMM:NF046476.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3336616.1"
                     /translation="MPTNKQDKTRIVQIWGDILDEGFTSVPNLLLRYRSALGIKPKHL
                     TLIIDIMSFKWDRYSPFPSYSTLAKRAGVEERSIKRITQDLEELHLLIKEPRFNEHTG
                     AQITTIFDFRPLVKKLTEEMLRADYNEEITDKDTEDLDSKGDDRIVMGGGDKNVTGGV
                     TKLSRGGVTKMSPKEYSYINKTQINKRTYSVKTHKNEKKVHTNLKNGIFKTRLYEIYD
                     NLNGVKPLEDVVEEGAYKTIKEIMVNRMTELSEENPEIDIDSIANLVKTDFPYDELPN
                     ESEKARKFFVEMLCDMVIDNIYLKAEGS"
     gene            7851..8657
                     /locus_tag="AC159U_05860"
     CDS             7851..8657
                     /locus_tag="AC159U_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3336617.1"
                     /translation="MKILAIANHKGGVGKTTTAVNLAACLAEKEQRVLLVDLDPQGNA
                     TSHLGYNPNDFELTINDVLLDTRGSIDINNVIIKRDDGISFIPANLSMSRADLSMSSL
                     IEKDHKLQKSLNKVKHKTDFVIIDCPPSLATLTINAFFAADDVIITIQTQPMALDAVK
                     MIDETLYEIYSSRTDYPIVPYGLPTMHDKLTNVSKTVIDAIEKKFGPQTLPAIHNNVR
                     LKEASGFGKHILEYDPLCSGAMDYRRVAKEIIRIYQDEQKEGRQYLSRSL"
     gene            8614..9237
                     /locus_tag="AC159U_05865"
     CDS             8614..9237
                     /locus_tag="AC159U_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336618.1"
                     /translation="MSKKKEDNTSVEASNKQKKKGRLDGMIESMNKRSDPLTTHKVAT
                     KYFSSFRSERNIDKPQLSEHIQGVTKHPTAVDSAVTTAVHTKVDTAVTNKNITTKPIE
                     QNSLSATHTNSENKIYNAIKSELIKSGSQEVRIGITRIRELTGLSDKTIRVAIKSLEH
                     KKSIVITKPSQGVYGRQYFLPESSEVVKERLEHGVQIDWASKHILPR"
     gene            complement(9246..9821)
                     /locus_tag="AC159U_05870"
     CDS             complement(9246..9821)
                     /locus_tag="AC159U_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3336619.1"
                     /translation="MAQNLPETLNYQERELLLSQPNQKAPTGLRDLAMIRLMINAGLR
                     SCEVLKMFIEDIDWISGKLHIKQGKGKKDRIIWVNEEDLNVIKKWKEIKPKSPVLFTT
                     LKGGCINDRYLRAMVKRRAQNAGITKNVHPHLLRHTFATDLLRSTKNIRLVQKALGHA
                     NLSSTMIYTHVYDEELESALKSFRNSNYRGS"
     gene            10043..>11108
                     /locus_tag="AC159U_05875"
     CDS             10043..>11108
                     /locus_tag="AC159U_05875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3336620.1"
                     /translation="MFRDTIRDFAANEVEPLIEEYEAKEDFPKELFPKLGEMGFLGIV
                     FPEEYGGVGMDKITDCIFSEEMGKVNAGMTMCINAHVGLSMFPIYKFGTDAQKEKYLI
                     PGIEGKIIGSLGLTEPNAGSDARSIRTQAVKEGDKYILNGTKTWITNGNMCDYAIVAA
                     YTDKTKKGEGISLFIVDKDSPGFQVGAKIHKLGHKAADTGELIFENCEVPEENLLVGQ
                     GGFGGAMGTLLGARITHAAKSVGIAQAALDYALQYSKEREAFGRPISKFQAISFKLAD
                     MAVKVESARLMVYKAAWLYSNDKKCIKEASMAKLYAAEVVQEVATEAVQVLGGYGYGV
                     EYPVERYYRDAKLASLTEGTS"
BASE COUNT         3641 a         1995 c         2268 g         3204 t
ORIGIN      
        1 taactgaagg cacatcagag gttcagagga tagttatctc aagagaactt tgtatataga
       61 gcttcgtatt gttttagaat gcatcaaaaa gtataattta tacaattgtg tcttaagtag
      121 ctgataaggt tgacttttgt aaaggtcaat ttaatataag agcctttgaa aattaaatcc
      181 tatatgataa gggggtagta gtaatggcaa gtacaatccc gggcattaaa gatgccagag
      241 aaatacttaa aaaaaataaa atcgttattg caggagtagg tgagaccgaa cagggcaaga
      301 tccctaataa aagctcattt cacttcctct ccgaagcatc aaagcttgca atagaagatg
      361 caggaattaa aaagaccgat gtagacggac tagtaacagc attttcactt gttgaacata
      421 cattcatgca ctgtactaca tttgcagatt attttggact aaaccccaaa ttcttttcat
      481 ctatagcaat tggtggttct acggcagtgt ggatggcagc agaggctgct atggctatcg
      541 ctaccggaca ggccgaggct gtgctttgtg tgagaggaga taatacactc tcaggaatat
      601 catcctccgg tatggtggcg cttattagag agatgtgcca tgcagagttt gagtatccat
      661 atggacttac aacaccaggt ggttatgcac ttgctgcaca gaggtatctg catgagtatg
      721 gagcaacctc agagcacctg gcagctattg cagtaacaat gcgtaagcat gcagctttga
      781 aagaaaatgc tatgaataaa gagcctctta ctatagatga tgttcttagc tccagggtta
      841 tcgcagaacc attacacaaa tatgattgtt caattatttc cgatggtggt gctgctttta
      901 tagttacaac ggaagaaaaa gcaaaacagc ttggaataga aaaaccactt gcatacctat
      961 ggggtatggg acagggctat tcacatcagt atctaactac actacaagat ttagaccaga
     1021 tatacaatgc agtagaaaga tctgggaaga aagcatacga tacagcggga ataggtccaa
     1081 aagatataaa cctggcatat ctttatgact gttttaccat aacagttgcc ctagagatgg
     1141 aaggtctcgg gtttgttggt aagggtgaag caggaccatt cgcactagaa ggccgcatgg
     1201 aaattggaaa agatatgcct gtgaatacaa atggagggct actgtcacaa gctcatttag
     1261 gagggatgca ccacgtaata gaagctacac tacaacttag aggtgaggca ggaccaagac
     1321 aggttgataa tgccgaagtt gcccttgttc atggaaacgg aggaattgtt tctgctcaca
     1381 gcaccatact gcttggaaac caagcgcttt cttaatcatt taggaggaaa ttaaatatgt
     1441 cagaagaaaa taaatacaat aaaccacttc cagagtttcg acccgaaact aaaccttttt
     1501 gggatgcatg taaaaaccat gaattaatgc tcccaaaatc aaaagaaact aacaaattct
     1561 ttttctaccc cagggctttt tctccaggag atgatatggc agaggtagaa tggagtaaag
     1621 caagcggaaa agcaaaggtt tggacatttt caattcatca tatgggccca acaaaggctt
     1681 ataaaggtga tggaccatat gtagttgcac tggttgaaac agaagaaggt gtaaaaatga
     1741 tgacaaatat agttgattgt gatcctaacg atgttcatat gggaatggat gtagaggttg
     1801 tatttgatga tgtaacagat gaagttacac ttccaaagtt taaacctgct gcataactaa
     1861 tacttgaagg agataattgt aatggcgcaa gatcgtattc tatatgaaga tttagaagta
     1921 gatgctgagc ataaaagctc cggcagaaca gttaccgaag cagatgttgt taattttgcg
     1981 ggcctttctg cagattataa taacatgcac atagatgaag agtttgctaa aaagacagta
     2041 tttaaaactc gtgtagccca tggaatgctg gtaacttcta ttgcaactgg tctttggttt
     2101 acaatgccaa ggcttgcaac agtggccttt atgggactcg aagactggcg tttctcaggt
     2161 gccgtattcc caggagatac tatccatata acgcgcaaat tagtagaaaa gagggaacat
     2221 aaacgcccca atatgggatt ttttatcttt gaagttaatg tacataacca aaaagacgaa
     2281 gtagtacaga aaggtaagtg ggtaattttg gtaaatagaa aagaatcatc ttaaaaacta
     2341 tatattttaa agtaaccaag gagcttttag aactccttgg ttacttcttc cattgtttat
     2401 ataaactaag tttaattttt ccaaccccaa attcctatct acagtaaaaa acatcttgac
     2461 atttatatat gaaatgtgct aaaagctact agtaatggtt atagtgtatc tttttgtgac
     2521 catacaactc ttataaacca taagaaaata aataaaggag gtatggagag atatggttcc
     2581 tgtagaggct ttacttggtt tagtgttatt attggtaggg ttcgttttct ttggaaacgg
     2641 gatgaccctt cttggtaaaa ccggtgctaa ggagattggt gtattaaacc ttggcgtagg
     2701 tattattatt tcaatagctg catggcagct acatggtctc ggtttaacag ccgctaccgc
     2761 tttagtatct gtatttgcac tgatttattt tatggtagcc ggaattttca ttcacggcca
     2821 cgatggtaag ggtttaggct ggtattgcct attcgctact atagtatttc tatggtacgc
     2881 atcacatttc agctcattag ggcttaccta tatggctggt ttctctgttg catgggctgt
     2941 tttagttttc tttgcttgga cagcacttgc tctaggaaaa ggaacagcaa cacttgttgc
     3001 ttatttattc cttatagagt ccatagtaac acttcttatt ccaggaatgt tgttactcac
     3061 agaaaaatgg aatccacttg gtggagtacc aggataatat atagtttata atatcaaact
     3121 atttttatat aagttttaac caaaaaaggg gcattatatg cccctttttt tattcctatt
     3181 aaagatttat tgtttgacct atacttaaaa caccccttta tttaaagatc tgtttaaacg
     3241 gatattttaa taaaaatact ataatagcag gtcttattaa catcgatgcg aatataaata
     3301 ggtcgcttgg ctttaggtaa atcttggaat cccatacccc catttgccct tttaacacaa
     3361 tagaatcctg ttctactgaa gaatcctcta ttgttatctc aaaatcccct gctggactta
     3421 caactttcat atatgtactc cttgtgttcg cgatatgata gaaataatat aaaactatat
     3481 caaatgattc attttaagct attttaacgc tgtttattga gttgataaga ggttagcaca
     3541 tagaaattag tatttaccaa taaaaaaccc cattagagct atatagttct aatggggtta
     3601 attttttaac tacctgtttt aattactatc cagacaaata attaaaagat agaagcacta
     3661 atagtgcttc tatccgttgt tttcaattaa ttaagcctgg ctgtactgtg gtagtaagaa
     3721 acgatgacgc attgtatatt caaatggttt tgaatataga atctgtccga aatctgcttc
     3781 gcctctttct acagttcttg tccagattgt agcagctggt ggaatgatca agttaaatgt
     3841 aaaactgata agaccaacaa tctctgttgc aggtatctga ccacccatga agtcaaatgg
     3901 agtacctgac atgtaccaag aacgaatatc atcgcctgta gctgtacgga tactctgagg
     3961 atctaactgg aatctatgtc ctgttccaaa tcctggaggc agctcagatg ggagatatgt
     4021 tacttcatgg atactatttg atacatctgg tcttgtggta atacgatagt tcattctacc
     4081 ccagtcagtt ttgccataaa atgtaggttt aactgaaagt ggtttgtctg aatagcgacc
     4141 attcgctgta tgtagtatat aaccacgacg ttccattaga tggttgaagt agttattgtt
     4201 tgttccatgc tcgaaaccag cgatataagc tgctttcttt ggaaatccaa atggctcttt
     4261 acctgcaaag attggtgagt catttgagag atacatatat ggataataac cagctttgta
     4321 aatatgtcct gaaccatcat ctactgtagc tgaaaaagtt acaccaaatt ccatgtatgg
     4381 tccaagcatt gtgtttctgt gaattacata ccagaactcg attacatcgt cttgtacttc
     4441 gattggttct ggaacgatag ctttcatagc atttatatca gctctgatac aatatgccat
     4501 ccagcggctt tctgtataag tcaatggaat aatatcatag tcaaatactg ggtcgataac
     4561 accctgactt atgatgtgta ttctgtctct atccattggt agaggtggaa cttcaccttc
     4621 tgccaaccat ggataactat attcattagc atgtaaaccc attgttataa cctcctgaat
     4681 cttttttttc gatatttatg tttatacaag aaactactat tatgatccct gactgattgg
     4741 gaactgctga cgtagtccat acttgtatgg tgttgcatat cctctatagc tggctggacg
     4801 ctcatatgtc tctgtccaaa gaacaactgc tggaggaata actagatcaa atacaaatga
     4861 aataagacct gtaacttcct gaatcttgat ctctgcaccc atgttatcaa aaggtgttgc
     4921 ctgagcaaac cagttaatag catcgccaga agctgtacgg gtatgttcag gtttaatttt
     4981 aaaacggtga ccctgaatca tttccttcat agcaccagga agctctgaag gtaggtatgt
     5041 tagctgccat tcggagctag atacgtctgg tcttgtgata accttcatgt taaatttacc
     5101 ccaatcggtc tttccataga aatatggagg ggttacctgc ttatcgtcga actgacctgt
     5161 agcactgtgt agtaaatatc cgttacggga cattgcaaag ccaaaaaagt cggttccttt
     5221 ttctccaccg tgaactgtgc atctgatata tgacattttc tttggaaatc cgagtacacg
     5281 accagcatct actggtgcat caccagtaag atacatatat ggataataac cagctctcca
     5341 cacattacct ttaggatctt tatagctggt tgaaactgtt acaccaaatt cgagatatgg
     5401 accaagcatt gtgttcttgt ggtctacata ccagtattca actaagtctg cgttttctcc
     5461 atcccataga tcgagaggtg gatcaggtac tagacgcctt cttgtaggct catcaaccct
     5521 gacaacaaaa gctgaccaac ggctgtctgt atatgtgaga ggaagaacat cgatatccca
     5581 tagtggatct gatggattag caataatcca ggattttatg tctctctcta taggaggagg
     5641 tggaacctcc tttgtgagtt cccaatattt cgtggggaca tttaaatgac taggcatatt
     5701 aaaaccctcc ttactaaata ttttttaaaa caggattaaa aaaatccccg ctgagcatcg
     5761 aatgcacgca aaccgcgcgt ttctataccc caacgacatt gttatatcaa aattacaagc
     5821 cctttgtcaa gtactttttt catatattca aaaaaagttt aagtaaaagc cttaaaatgc
     5881 aaaaaataag tgctatattg gcttatctag ctttcagtat cagtgatatt actttccaac
     5941 aagctcgcca ctaaggaagc aaacccacct atcttttccc gaacaataag cgttgcatct
     6001 gtatcaaaag gggtagcctg aatattgcat ataacaagtt ttgcgccttt tttgatagtc
     6061 atatcaggta gtatatttgc gggtgagact tgtagtgatg agcctattac aatgaacaag
     6121 tcagcgtttt cacaaagctt aactgattct ttccaaggta tttgcggtag agcttcacca
     6181 aaaaatatgg cgtttggctt caaaacagct ccacactcct gacagtacgg cggattttcc
     6241 ccagaaacta ccctttgcat aatttcttcc atgggaaaca acaagttgca gccaagacag
     6301 actgtctgcc taagagatcc gtgaagttca tgaacggttt cagaaccggc tttgtaatgc
     6361 aaaccatcta cattctgcgt aataacacct tttaaaaatc cgagtttttc taacgaggct
     6421 agaagataat gcgcaggatt tggctttgca gcgcttctta ccggataaag cttaagtgag
     6481 tatttgtaat attttgaagg gtctgctaaa aaaccatcta gagaaaccac agacatatcg
     6541 gtattttccc aaaggcccgt atccggtgaa cggtaatctg gtattccgga ctccgtgctt
     6601 ataccagcac ctgttagcgc tacagcatgt ttgcaatttt gtattagatc taaaaaatct
     6661 cttgccaatt tttcatttct gtccattggt tgttctcaga aaagtttttt gtattattaa
     6721 ttgctctagt gttatatatc aatctttgat ttaaatcggt aattataata taataaatat
     6781 aagaaagccg ccgatgtttg tggagaaaac aaaaacaacg gcggcaaccg gaaggtggaa
     6841 catctaaatg ataccttccc ataaaaagca atgtcaagac cttaatttat ttaagataaa
     6901 tttaggaagg taaatttgcc tacaaacaaa caagataaaa ccagaatagt acaaatatgg
     6961 ggcgatatac ttgacgaagg ctttactagc gtcccaaacc tcttacttcg ctaccgttct
     7021 gccctcggaa ttaaaccaaa acacttaaca cttataatag atatcatgtc ttttaagtgg
     7081 gatagataca gtccgtttcc aagctactcc acacttgcaa aaagggctgg agttgaagaa
     7141 agaagcataa aaagaatcac ccaggactta gaagaacttc atctgcttat aaaagaaccg
     7201 cgttttaacg agcatacagg ggctcagata acaacaattt ttgattttag acccctggta
     7261 aagaaactca cagaagagat gctgagagca gattataatg aagaaataac cgacaaagat
     7321 acagaagact tggattctaa gggggatgac agaattgtca tggggggggg tgacaaaaat
     7381 gtcacggggg gggtgacaaa attatcacgg gggggggtga caaaaatgtc acccaaagaa
     7441 tactcatata taaataagac acaaataaat aaaagaacct atagtgtaaa aacgcataaa
     7501 aacgaaaaaa aggtgcatac aaatttaaaa aatggcattt ttaaaacccg attatacgaa
     7561 atatatgaca atttgaacgg ggtaaaaccg ctagaggatg tggtagagga aggagcttat
     7621 aagacaatta aagaaataat ggtaaacaga atgactgagt tatcggagga aaatccggaa
     7681 attgatatcg actcgatagc aaatctagta aaaacagatt ttccttacga cgaattacca
     7741 aacgagtcag aaaaagccag aaaatttttt gtagaaatgc tttgcgatat ggttatagat
     7801 aatatctatc taaaggcaga aggcagctaa gaaaaaacga agggggaagc atgaaaatat
     7861 tagccatagc aaaccacaaa gggggtgtag ggaagacaac gacagccgtg aacttggcag
     7921 cgtgtctagc ggaaaaagaa caaagagtgc tccttgtgga tttagatccc cagggaaacg
     7981 ctacttcgca cttgggatat aacccaaatg attttgagct taccataaac gacgtgctgt
     8041 tggatacaag aggcagtatt gatataaaca atgtgataat taaaagggat gatggaataa
     8101 gttttatacc ggcaaaccta tctatgagca gagcagatct aagtatgtct tctttgatag
     8161 aaaaagatca taaattgcag aaatcattaa ataaagttaa acataaaaca gattttgtta
     8221 taatcgattg cccaccttcc cttgcaacac ttactataaa cgcatttttt gcagcggacg
     8281 atgtgataat cacaatacag acacagccaa tggcccttga tgctgtaaaa atgatagatg
     8341 aaacgctata tgaaatatat tcatccagga cagattatcc gatagttcca tatgggcttc
     8401 ccactatgca tgacaaactt acaaatgtaa gcaaaacggt aatcgatgca attgaaaaaa
     8461 aattcgggcc acagacgctc cctgccatac acaacaatgt aaggcttaaa gaagctagtg
     8521 gttttggcaa acatatttta gagtacgacc ccctgtgctc gggcgctatg gactacagaa
     8581 gagtagcaaa agagataata aggatatatc aggatgagca aaaagaagga agacaatacc
     8641 tcagtagaag cctctaataa acagaagaaa aagggccgcc tggatgggat gattgagtcc
     8701 atgaacaaac ggtctgatcc acttaccaca cataaggtag ctacgaaata tttcagctct
     8761 tttaggtcag agcgaaatat agataaacca caattaagcg aacatataca aggggttacc
     8821 aaacatccta ccgcggtaga tagtgcggta actactgcag tacatactaa ggtagatacc
     8881 gcggtaacta acaaaaatat aaccacaaag cccatagagc aaaatagtct aagtgctaca
     8941 catacaaatt ccgaaaataa aatctataat gctataaaaa gcgaattgat aaaaagcggt
     9001 tctcaggagg tcagaatagg tattacaaga atacgcgaac tcacaggcct ttctgacaaa
     9061 acaataaggg ttgctatcaa atcattggaa cataagaaaa gtattgttat cacaaagcct
     9121 tcccaagggg tgtatggtag acagtacttt ttaccagaat catctgaagt ggttaaggag
     9181 aggttggagc acggagtaca aatagattgg gctagcaagc atatattacc gaggtagcta
     9241 ccgcactaac taccgcggta attactattc ctaaatgatt ttaatgcgct ttcaagctct
     9301 tcatcataca catgggtata aatcatggtc gatgacaggt ttgcatgccc aagggctttt
     9361 tgaacaagcc ttatattttt tgtagacctt aaaagatcgg ttgcaaatgt atggcgcagt
     9421 aagtgagggt gcacgttttt agtgattcca gcattttgcg ctcttctttt taccatagcc
     9481 ctcaaatatc tgtcatttat acatcctcct tttaatgtgg taaacaaaac aggactttta
     9541 ggctttattt ctttccattt ttttattacg tttaaatcct cttcattaac ccatattatt
     9601 ctatccttct taccttttcc ctgttttatg tgaagttttc cacttatcca gtctatatct
     9661 tcaataaaca tttttaatac ttcacaagaa cgcagaccag catttatcat aagtcttatc
     9721 atggcaagat ctctcaggcc agtaggggct ttttggtttg gctgagaaag tagcaattcc
     9781 ctttcttgat agtttaatgt ttccggtaaa ttttgtgcca taggttttca ctgaagaata
     9841 ccatatttgt ataaagacgg tattatatac agcttaatta tatatacttc tcggaattta
     9901 cgaaatacgg tactatataa cattttctgt actatttaca cagtctatac aaacgtgata
     9961 taatcaagcc gccaaattaa atctttatta caataggagg acaaccgtgt cactcaattt
    10021 tgaatttaca gaagaacaag agatgttcag ggatactatt agggattttg ctgcaaatga
    10081 ggtggagcct cttatagaag aatatgaagc taaagaggat tttccaaaag aactatttcc
    10141 caagttgggg gaaatggggt ttttggggat agtgtttcca gaagagtatg gtggagtagg
    10201 aatggataag attactgact gcatattctc tgaagaaatg ggaaaggtaa atgccggtat
    10261 gaccatgtgt attaatgcac atgttgggct ttcaatgttc ccaatatata aatttggtac
    10321 agatgcacaa aaagaaaaat atcttattcc aggcatagag ggaaagatca taggatcctt
    10381 ggggcttaca gagccaaatg caggttctga tgcaagaagt atacgcacac aggctgtaaa
    10441 agagggagac aagtacatat taaatggtac aaagacctgg attactaatg gaaatatgtg
    10501 tgattatgca atagtggcag catatacaga taaaacaaaa aaaggagagg gtataagcct
    10561 ttttatagtg gataaggact cgccaggttt ccaagtgggg gcaaaaatac ataaattggg
    10621 ccataaggct gcagacactg gagaactaat atttgaaaac tgtgaagttc ccgaagagaa
    10681 tcttttggta ggacaaggag gatttggtgg agctatggga actctccttg gagcaagaat
    10741 cactcacgct gctaaatcgg tgggaatagc tcaggcggca cttgattatg ctctgcagta
    10801 ttcaaaagaa agagaagcgt ttggaagacc aatttccaag tttcaggcta taagctttaa
    10861 actagcagat atggctgtaa aggtagagtc tgctaggctt atggtgtata aggctgcatg
    10921 gctttacagt aatgataaaa aatgcataaa agaagcatcc atggctaagc tttacgcggc
    10981 agaagtggta caggaagtgg ctacagaggc tgtacaggtt ttagggggtt atggttatgg
    11041 agtggagtac cctgtagaga ggtattatag agacgccaag cttgcatctt taactgaagg
    11101 cacatcag
//
LOCUS       DBNGSV010000163         3662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1067842, whole genome shotgun sequence.
ACCESSION   DBNGSV010000163 DBNGSV010000000
VERSION     DBNGSV010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1067842"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..702
                     /locus_tag="AC159U_05880"
     CDS             217..702
                     /locus_tag="AC159U_05880"
                     /inference="COORDINATES: protein motif:HMM:NF021996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR3336621.1"
                     /translation="MNKRVIGTILVIIVAVVWYLFRPERLIIDSKVNEDFPSVQNDKN
                     EIKPQILLKGTFYAVAHPAKGTATVYKLSDGKRILRFTNFEVSNGPDVVVYLSSVIDA
                     NDNELVKNSKIISLGSIKGNVGDQNYELPSDIDFNIYKSVVIWCKRFGVNFAVAPLTS
                     S"
     gene            807..2414
                     /locus_tag="AC159U_05885"
     CDS             807..2414
                     /locus_tag="AC159U_05885"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR3336622.1"
                     /translation="MRTLFIVISIVVVVVISLGGMFLSPKIFLSFIVVIPLLALGLWD
                     YFQTKHAVLRNFPIVGHARYILELIRPEINQYFIESNTDGVPFSREQRTIVYQRAKKV
                     LDTLPFGTQKNVYEIGYEWLNHSLKPVHVDMKDLRVTIGGPQCKKPYSASILNISAMS
                     FGALSSNAVMALNEGAKMGNFAHNTGEGGISSYHKMGGDLIWQIGTGYFGCRTEDGCF
                     CPKQFEEKSKMDQVKMIEIKISQGAKPGHGGILPAAKITPEIAEIRSVPMGKDVNSPP
                     AHKEFLTPFGLMEFVKKLRDLSGGKPIGFKLCIGKRREFLAICKAIVKTGIYPDFISV
                     DGGEGGTGAAPLEFSNHLGTPLTSGLIFVHNALVGFSLRDKIKVISSGKIASGFGIIK
                     QLCIGADLCYSARAMMMALGCIQALRCNSNHCPVGVTTQNPELVAGLVVSDKKQRVAN
                     YHYETIESVAEMIGAMGISKTEDLRNWHITKRVGPNELKHYGEIYHYLNDGDLLKEEL
                     PKDYARACKAAVAESFDHIEDISKRFN"
     gene            complement(2506..3018)
                     /locus_tag="AC159U_05890"
     CDS             complement(2506..3018)
                     /locus_tag="AC159U_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336623.1"
                     /translation="MRKLIIFASLFGLLLLLNTNPTLAKDKYYDGHGKKYNKSGHKKY
                     PHAEKHLKHDYYHYKQDRKHYKHHKYNDDYYHGYKKQHKRHYGHYKGHNKNYGHDHEY
                     NYYKSKRHHRHKNHHVKHHKKHYHGDYHHRHHYKKHHDHYNYRYGHHASNHGYAYFNL
                     FPYLLGYFQY"
     gene            3150..>3662
                     /locus_tag="AC159U_05895"
     CDS             3150..>3662
                     /locus_tag="AC159U_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336624.1"
                     /translation="MYKSKEIILPVIVILLLLITPLWMICTYYGEYSYYDELSKDGIQ
                     TGARLDNKEIKTDKSGIFSLFIADSTENYRLTAGYYINTDSYIRCEFGVSKDTFYSIG
                     LRDELLVVYPPSNPTKCTLTDGVEISRYFLLITLIFALFILILGIGFIYYVYKSYAKP
                     TPDKLIKPTTN"
BASE COUNT         1227 a          533 c          692 g         1210 t
ORIGIN      
        1 catatatact actcctaaca acgtctaatg ccctagaata ttatctaaat tatatccagt
       61 aataattaaa tataattaac attaatcaat atgaatctca ataataaaaa attatctctt
      121 aaatgtagag tctgcaaata atatatataa atctcaatat aatagcagga ataactgtat
      181 ttattcactg aaaaaattaa tatcgaggag aagagaatga ataaacgagt gattggtaca
      241 attttagtca ttatcgtagc agtagtttgg tatcttttca ggccggaaag attgataatc
      301 gattcaaaag ttaatgagga ttttccttcc gtacaaaatg acaaaaatga aattaaacca
      361 caaatactac ttaagggaac attttatgca gttgcccacc ccgcaaaggg tactgctacg
      421 gtttataaac tatccgacgg taaacgcata ctcagattta cgaattttga ggtatcaaac
      481 gggccggacg tagtggtata tttatcctct gttatagatg ctaatgataa tgaattggtt
      541 aaaaactcaa agattatttc gcttggttca attaagggca atgttggaga ccagaactat
      601 gagcttcctt cggatattga ttttaatatt tataagtcag ttgtaatatg gtgtaaaaga
      661 tttggtgtta attttgctgt tgcaccttta acttcaagtt agaaaagaac atttgtagta
      721 aagaacataa tataaaatta atattaaaga ttgtatggtg atggagtatt atattaaaat
      781 tattttaata attggagcat aatactatga gaactctttt tatcgtaatt tccatagttg
      841 ttgtagtagt tatatcgctt ggaggaatgt tcttaagtcc aaagatattt ttaagtttta
      901 tagtcgtcat tccacttctg gctttaggtt tatgggatta ttttcaaaca aagcatgcag
      961 ttttgaggaa ttttccaata gttgggcatg cgagatatat cctcgaatta ataagacctg
     1021 agattaatca gtactttatc gaatcgaata ccgatggagt tccgtttagc agagagcagc
     1081 gtactatagt ttatcagaga gcaaaaaaag ttctcgatac tttacctttt ggaacacaaa
     1141 aaaatgttta tgagattggg tatgagtggt taaatcattc attaaaacct gttcatgtgg
     1201 atatgaaaga cttaagggtt actattggcg gtccacagtg caaaaaacct tattcagcta
     1261 gtattctaaa catttcggct atgagttttg gtgctcttag ctcaaatgcg gtcatggcgc
     1321 taaatgaagg tgctaaaatg ggtaattttg ctcacaatac cggtgaggga ggtattagtt
     1381 cttatcacaa aatgggtggg gatttaatat ggcaaattgg tacgggatat tttggctgca
     1441 gaactgaaga tgggtgcttt tgtccaaagc agtttgaaga aaaatcaaag atggatcagg
     1501 tcaaaatgat agagataaaa atatcgcagg gtgcaaaacc tggtcatgga ggaatactcc
     1561 ctgctgcaaa aatcactccc gagattgctg agattagaag tgtacctatg ggaaaagatg
     1621 ttaactcacc cccggcacat aaagaatttt taaccccttt tggcctaatg gaatttgtga
     1681 aaaaactaag agacctatca ggtggtaagc caataggttt taaactctgt attggaaaga
     1741 gaagagaatt cttggcaatc tgtaaagcaa ttgttaaaac aggtatttat ccggatttta
     1801 tatcggtaga tggtggagaa ggaggcacag gggcagcacc tctggaattt tcaaaccatt
     1861 tgggaacgcc actaacctca gggctgatat ttgttcataa tgctcttgtc ggattttctt
     1921 taagagacaa gataaaggta atatcaagcg ggaaaatagc ctctggtttt ggaatcatta
     1981 agcagttgtg cataggtgcc gacctttgtt attctgcaag ggcaatgatg atggctcttg
     2041 gatgtattca ggccttaaga tgtaattcca atcactgccc ggtgggtgta actactcaaa
     2101 atcctgaatt ggttgcaggg cttgtagtat cagataagaa acaaagagtg gcaaactatc
     2161 attatgaaac cattgaaagc gtagctgaaa tgattggagc tatgggaata tcaaaaaccg
     2221 aagatcttag aaactggcac atcacaaaaa gagtcggacc taatgagcta aagcactatg
     2281 gggaaatcta tcattattta aatgatggtg atttgttaaa agaagaacta ccgaaagatt
     2341 atgcaagagc atgcaaggca gcagttgcag aatcgtttga tcatatagaa gatatttcta
     2401 aaaggtttaa ttaattaaat aattacttga tgaattaaca ataaagaccc tcgttcaatt
     2461 agagggtctt tatcctaatt aatcaggcaa ttcatttata aatgatcaat attgaaaata
     2521 ccccagtaaa tatgggaaaa ggttaaagta tgcataacca tgatttgatg catggtgacc
     2581 atatctatag ttgtagtggt catgatgttt cttatagtgg tgtctgtgat gatagtcacc
     2641 atggtaatgt tttttatgat gttttacatg atgattcttg tgtctatgat gtctctttga
     2701 tttataatag ttgtattcat gatcatggcc gtaattttta ttatggccct tataatgacc
     2761 ataatgcctc ttgtgttgtt ttttgtatcc atgataataa tcatcattgt atttatggtg
     2821 cttataatgt ttcctgtcct gcttataatg ataataatca tgctttaagt gcttttcggc
     2881 atgtggatat tttttatgac cacttttgtt atacttttta ccatgtccgt cataatactt
     2941 gtcttttgct agggttgggt ttgtatttag caaaagcaat aaaccaaaca gacttgcaaa
     3001 tataattaat tttctcattt catttacctc ctaaatcata ttttgcatat ataaactgat
     3061 atcatttcct ttagtttcat aaaataaatt gataagttta gaagctgtga ttaaaatggc
     3121 atcataagaa aagttaatta attcaagaaa tgtataaatc aaaagagata atactgcctg
     3181 ttatagttat attgctgctg cttattactc ccttgtggat gatctgtact tattatggag
     3241 aatattctta ctatgatgaa ttatctaaag acggtattca gactggtgcc cgccttgata
     3301 ataaagaaat taaaactgac aaatccggaa tattcagttt atttatagca gacagtacag
     3361 aaaattatag gcttactgct ggttattata taaatacaga cagttatata agatgtgaat
     3421 ttggtgtatc caaagatacc ttttactcca ttggattgag ggatgaatta ttggtggtat
     3481 atccaccatc taatcccaca aaatgcactt taacagatgg agtagaaata agcagatatt
     3541 ttttattaat tacattaata tttgcactat ttattttaat actgggtatt ggatttatat
     3601 actatgttta taaatcttac gctaaaccta ctccagacaa actcataaaa cccacaacta
     3661 ac
//
LOCUS       DBNGSV010000164         5492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_860201, whole genome shotgun sequence.
ACCESSION   DBNGSV010000164 DBNGSV010000000
VERSION     DBNGSV010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_860201"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="AC159U_05900"
     CDS             complement(<1..1239)
                     /locus_tag="AC159U_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR3336625.1"
                     /translation="MAKVISSKQTETGFDLSKYYVEHFEHIKKLHRLKAGNLNKLSGR
                     SLAKRRSKAMDDLIINYLSYGGFTNLKNLSVIALGGYGRSDLSPHSDIDILFLYDDIF
                     IKTAKEITQSLLYKLWDLKLDVGYATRTVDECVELSFDKDTTILTSMLDSRLIYGDES
                     LFKSLNNKLFKQVLPKNSKTFIENKVSENLQRIEKYGNSIYMLEPNVKESVGGLRDIH
                     FALWIAQAKFKVKTFKELLLKGILFHNELNTLNKNMDFLLSIRAELHYLAGKAEDRIR
                     FEYGEDIAKYLGFKDSDLRAVEKFMRIYYLRANQTREVSKRLIDRCLVKPQISSKFSK
                     ITKLGDGFILQGGKLSVTHRKIIEDQPPNMIRAFVISDKFGCNKTPYLADLIKDNSNL
                     KTITDKFRTNEEVNQYFLKLL"
     gene            complement(1250..1900)
                     /locus_tag="AC159U_05905"
     CDS             complement(1250..1900)
                     /locus_tag="AC159U_05905"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3336626.1"
                     /translation="MNKIDTYDTQIVNPEIEQYLNNLLADDDPLLREMEQLALNMDFP
                     IIGPQVGRLLYQLTKISGAKKILELGSGFGYSAYWFSKALNDDGYVICTEHSEDNHKI
                     AEKFFKKSEYFNRVRYEKGEALSILDKQEEQFDIILNDIDKEYYPDVIEIAAEKLKPG
                     GLLITDNMLWFGRVLSDYKTPSTAGVKIFTELILERKDFMTTIIPLRDGVAISLKV"
     gene            complement(1906..2466)
                     /locus_tag="AC159U_05910"
     CDS             complement(1906..2466)
                     /locus_tag="AC159U_05910"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3336627.1"
                     /translation="MPKKRITKVYTKTGDDGYTSLVFGQRVSKASLRVTAYGDVDELN
                     SVIGVIRSKNTDKKIDVLLTNIQNDLFVIGADLASVESDKSLRLSKKRVAFLERNIDK
                     YLKHLEPLKEFILPGGSEAGSLIHLGRTVARRAERNVTALMDSEKVNNLVLIYLNRLS
                     DLFFVLARIVNKAENKPETYVDFNKN"
     gene            2626..3771
                     /locus_tag="AC159U_05915"
     CDS             2626..3771
                     /locus_tag="AC159U_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3336628.1"
                     /translation="MADKEIYLDNNATTRPYPEVVTAMMEVLGEGFGNPSSTHATGQR
                     AKEFVERAREETAKLLGCDKDKLVFTSSGTESNNMAFYSCFRDCKEKCRIVTSCVEHS
                     SIKKMCSYLKLNGAEIIELDACEGGYVNLDVLDKTLGDKKVSLVSIQWVNNETGVIQP
                     VEEIGKICRKHGILYHTDAAQAVGKLELNLDSMPIDYLSLTGHKFNSPQGIGAIYCKN
                     KLNLNPIMFGGFQEDHFRPGTENVPGIVGIGKASEIRRKKLKDNIAYMTELRDSFEEQ
                     LSQEIRCVSFNGDTENRVCNTSNVQFKNIDGRILKARLDSVGIRCSQSSACTNSSPDP
                     SYVLLAMGKSPEDAYSSIRFSFGTSNTMEEIEQTVAHIKKFCRKLKH"
     gene            3790..4176
                     /locus_tag="AC159U_05920"
     CDS             3790..4176
                     /locus_tag="AC159U_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336629.1"
                     /translation="MTENGFNRAKALEEGHQIDISDHVKNAGLLIPAYVTKQLWNEVI
                     KLDATTNNLNTEEDKRIKEVISRLVYYIRTHRQSSKSNTIYFNVEFKREGEQQTYDLV
                     SHLGVIDDENSDPCLTLFMSGEGQTQ"
     gene            complement(4177..4650)
                     /locus_tag="AC159U_05925"
     CDS             complement(4177..4650)
                     /locus_tag="AC159U_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336630.1"
                     /translation="MNTEQTPNSIEPEGMFSGLNFRAIIVGVLADIFSTLILGMIITT
                     VLLVKLHGADIPEDAVEKILESDSNLYIYLLLGLCCTVCGGFMCARIAGSLPIRHGGW
                     VGAASLITGVILEMMLGADQTYPDWYIYISMAGIIPAGVLGGYLYNLLKSIDVSN"
     regulatory      4783..4872
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5025..>5492
                     /locus_tag="AC159U_05930"
     CDS             5025..>5492
                     /locus_tag="AC159U_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336631.1"
                     /translation="MSPCSSNIQFISFTDSNSGIKEVISSVNKYPNSLIFINPTVDNL
                     SEIERLLTKFNFLAAREIPIIALIKDFTNHNIKKLLSLGVSDFVYHPFTTQEIKARID
                     RYIDKSHEEIKTNLKQHLKQKLGLKNIIGQSPNFLSEIEKIPQIAQYDATVMIT"
BASE COUNT         1773 a          982 c          959 g         1778 t
ORIGIN      
        1 taaaagttta agaaagtact gatttacctc ttcgtttgtt ctaaatttat cggttattgt
       61 ttttaaattt gagttgtctt ttattaagtc cgcaagataa ggtgtcttgt tacaaccaaa
      121 tttatcggag atgacaaatg ctcttatcat atttggaggt tggtcctcaa taattttcct
      181 gtgtgtaact gataattttc ctccctgaag tataaaacca tcacctagtt tggtaatttt
      241 ggaaaacttt gaagatattt gtggttttac taaacatctg tctataagcc ttttggatac
      301 ttctcttgtt tggtttgcac ggaggtagta aatcctcatg aatttttcca cagctcttag
      361 atcactgtct ttaaatccca gatatttggc tatgtcttca ccgtattcaa acctgatcct
      421 gtcttctgct tttccggcaa gatagtgaag ctcagctctt atagacagca gaaaatccat
      481 gtttttattt agtgtgttta attcattatg aaataatatt ccctttaata aaagctcctt
      541 aaatgttttt accttaaatt ttgcctgcgc gatccagagt gcaaaatgaa tatccctaag
      601 tcccccaaca ctctctttta catttggctc gagcatgtat atggaatttc catatttttc
      661 gatcctttgc aggttttctg aaactttatt ctcaataaaa gtttttgagt ttttaggaag
      721 aacctgctta aacagtttat tgttaagtga tttaaataat gattcatccc catagatcaa
      781 tctggagtca agcattgaag ttagaatggt cgtatcttta tcaaaggaca gttcaacgca
      841 ttcatcgact gttctagttg cgtatccaac atcaagctta aggtcccaaa gcttatagag
      901 aagactctga gtaatctctt ttgcagtttt tataaatata tcgtcataaa gaaacaggat
      961 atctatgtct gagtgtgggc ttaaatcgct tcttccataa cccccaagcg ctatgacgct
     1021 taggtttttt agattagtaa atccaccata gctaagataa ttaataataa gatcgtccat
     1081 tgctttggac cttctcttag caagcgatct gccagaaagt ttattcaaat ttccggcctt
     1141 aaggcggtgc agttttttta tgtgctcaaa atgctctaca taatacttgc ttagatcaaa
     1201 ccccgtttct gtctgttttg aagatattac ttttgccatt tgttgtgaat tatactttta
     1261 agcttattgc cactccatct cgtaaaggaa ttatagttgt cataaagtct tttctttcta
     1321 aaattagctc agtaaaaatt tttactcctg cagtcgacgg agttttataa tcagaaagaa
     1381 ccctgccaaa ccataacata ttgtccgtaa ttaacagacc accgggtttt agtttttctg
     1441 ctgcaatctc aattacatcc ggataatatt ccttatcgat atcatttaga atgatatcaa
     1501 attgttcttc ctgtttgtct agaatagaca aagcttcccc tttttcatat cttacccggt
     1561 taaaatactc acttttttta aaaaattttt ctgctatctt atgattgtcc tcagagtgct
     1621 ccgtacaaat cacatatcca tcatcattta atgctttgga aaaccaatag gccgaatatc
     1681 caaatcccga tccaagctcc aatattttct ttgcacccga tattttggtt aattgatata
     1741 ataatcttcc gacttgcggt ccaataatcg gaaaatccat attcagggca agctgctcca
     1801 tttcccttag caaagggtca tcatcggcca aaagattatt taaatattgt tcaatttccg
     1861 ggtttacaat ctgtgtatca taagtatcaa ttttattcat gattattagt tcttattaaa
     1921 atctacatag gtttctggtt tattctcggc tttgtttaca atccttgcta atacaaaaaa
     1981 caggtctgat agacggttaa gataaattaa aaccagatta tttacttttt cagagtccat
     2041 taaggctgta acatttctct cggctcttct tgcaacggtt cttccaagat gaattaatga
     2101 tcctgcttcg ctacctccgg gaagtataaa ttcctttagt ggttctaagt gttttagata
     2161 tttatctata ttccgctcta aaaaagcaac ccgctttttg gaaagcctaa gtgatttatc
     2221 cgattctaca gatgctaaat ctgcacctat aacaaataga tcattttgga tatttgttag
     2281 cagcacatcg atttttttat ctgtattttt ggaccttatt acaccaatta cactgttaag
     2341 ctcgtctaca tccccgtagg ctgtaaccct caatgaggct ttgcttaccc tctgtccaaa
     2401 cacaagggat gtatatccgt catctcctgt tttggtataa actttagtta tccttttttt
     2461 aggcattgtg caagtttaac tggggtgttt attaactcaa cttacactca tttaatgcct
     2521 gacggattaa tataattcat cacagcaaat cattataaaa ttgtattaat atatttagtt
     2581 ttattaatta atgtatatat tatattttta agggtagaga acaaaatggc agacaaagaa
     2641 atatacttag ataacaacgc aaccaccagg ccttatccag aagtagtgac ggccatgatg
     2701 gaggtattag gtgagggatt tggtaatcct tccagtactc atgcaaccgg gcaaagggca
     2761 aaagaatttg ttgaaagagc cagggaagag acagcaaaac tcctaggatg cgataaagat
     2821 aaactagtgt ttacaagctc gggaacagag tcaaacaata tggcctttta ctcatgtttt
     2881 agggattgta aagagaaatg ccgtatcgtt acatcttgtg tagaacattc atcgataaag
     2941 aagatgtgca gttatttaaa gctaaatggt gcagagatca tagagcttga tgcctgcgag
     3001 ggtggatatg tgaatttaga cgtgcttgat aaaacactgg gtgataagaa agtgtcgctt
     3061 gtttctatcc aatgggttaa taatgaaaca ggggtaattc agcctgtaga ggagattggt
     3121 aagatatgca gaaaacacgg aattctttat cataccgatg cagcacaggc agtagggaaa
     3181 ttggagctta atcttgatag tatgcctatt gattaccttt cgctcacggg tcataaattt
     3241 aattctcccc agggtatcgg ggctatttac tgtaaaaata aattaaattt aaatcctata
     3301 atgtttggcg gctttcagga ggaccatttc agaccgggta cagaaaatgt tccaggaata
     3361 gtgggcatag ggaaggcttc ggagattaga aggaaaaaat tgaaggataa tatagcatat
     3421 atgacagaac tgagggacag ttttgaagaa caattgtctc aggaaatacg ctgcgtaagc
     3481 tttaacggag atactgaaaa cagggtgtgc aatacttcaa atgtacagtt taaaaacata
     3541 gacggcagaa ttcttaaggc aaggctggat agtgtgggaa ttagatgttc tcaaagctcg
     3601 gcttgtacca attccagccc cgacccatca tacgttctgc ttgcaatggg aaaatctccc
     3661 gaagatgctt attccagcat aagatttagt tttggtactt caaatacgat ggaagaaata
     3721 gaacagaccg tagctcatat caagaagttc tgccggaaac ttaaacatta aactctttat
     3781 ggtgaaatta tgacagaaaa cggatttaac agagcaaaag ccttagaaga aggccatcaa
     3841 atagatattt cggatcatgt aaaaaacgca ggacttctaa ttcctgctta cgttacaaaa
     3901 cagttatgga atgaagtaat taaactcgat gcgaccacta ataatcttaa tactgaagaa
     3961 gataagagaa ttaaagaagt tataagcagg ctggtttatt atattagaac tcatagacag
     4021 tccagtaaat ctaacacaat atattttaat gtggaattca aacgtgaagg tgagcagcaa
     4081 acctacgatc ttgtatctca tctgggtgtt atagacgatg aaaactcaga cccatgttta
     4141 acacttttta tgtcaggaga aggacagact cagtaactaa ttagacacat caattgattt
     4201 tagcaaattg tataaatacc cacctaaaac accggcggga ataataccgg ccatagagat
     4261 ataaatatac cagtcagggt atgtttgatc tgcaccaagc atcatttcca atattacacc
     4321 cgttattaaa gaggcagcac caacccagcc gccgtgtctt attgggagtg aaccagctat
     4381 tctggcacac ataaatccac cacacactgt acagcatagt cctaataaaa gatagatata
     4441 aagattgcta tctgattcca gaattttttc tacagcgtct tctggtatgt cagccccatg
     4501 tagttttact aacagaactg ttgtaattat cattcctaga ataagagtag aaaaaatatc
     4561 tgcaagaaca ccaacaataa tggccctaaa gtttaaccct gaaaacatgc cctcgggttc
     4621 aattgaattt ggagtttgtt ctgtattcat ggtggtgaga ttacattatt atcgtaaaaa
     4681 cctacaatta aatatattta ttattaaaat tataataatc accgaaaata cgtattgaca
     4741 ttatcaatac taaagtaata taatatatat tgaaggaggt ttatacgata aaggcaaacc
     4801 atttgaaaag atgggacgca aagctacttg cctcaagcat caacatgatt gccaggtaga
     4861 aggatcaccg aaataaaatt cttcatattg aatttgcaat aaatatattt tattaacgga
     4921 ttcgataggg ggatattatc ggtattagta atgctacctt tctacttctg gatatcggtt
     4981 cagagcaata ttcttgcagt gaaaatgatg cgcttgtaaa tattttgtcg ccctgtagta
     5041 gtaacattca atttatctcg tttaccgatt caaactctgg tattaaagaa gtaatctcat
     5101 ctgttaataa atacccgaat agtcttattt ttattaatcc tacagttgat aacttatcag
     5161 aaattgagcg gctgctaacc aaatttaatt ttttagcagc aagagaaatt cctataattg
     5221 cgcttattaa ggattttact aaccataata taaaaaaact gctaagcctg ggtgtaagtg
     5281 attttgttta ccatcctttt acaacacagg agattaaagc ccgaatagat agatatattg
     5341 ataaaagtca tgaagaaatt aaaacaaatc taaaacaaca ccttaagcaa aagcttggtc
     5401 taaaaaatat cataggccaa agcccaaatt ttttatctga aattgaaaaa atacctcaaa
     5461 ttgcacagta tgatgctact gtgatgatta cc
//
LOCUS       DBNGSV010000165         2152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_860218, whole genome shotgun sequence.
ACCESSION   DBNGSV010000165 DBNGSV010000000
VERSION     DBNGSV010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_860218"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC159U_05935"
     CDS             <1..412
                     /locus_tag="AC159U_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3336632.1"
                     /translation="IGCLSPEDYRTYVFPHMKTLFSSIDKNIPTVHFGTGTSSLLGLM
                     KQAGGTVIGFDWRVDINKAWKEIGYTCSVQGNIDPIVLFSTPSIIRKKVKELLKKVNG
                     RRGHIFNLGHGVLPGTPPDNVLALIDAVHEYSSR"
     gene            421..1356
                     /gene="hemH"
                     /locus_tag="AC159U_05940"
     CDS             421..1356
                     /gene="hemH"
                     /locus_tag="AC159U_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3336633.1"
                     /translation="MGKDFNSILMIGYGGPDKPEDIMPFLENVAKGFNIPRSRLEDVA
                     HHYELIGGKSPLNELTYRQAIGLEHQLAAMGHDLPVFIGMRNWDPFIPDTLRIMKREQ
                     TLNSVGIILAAHKCDASWEKYMRDIEQAKEDTDIDLNIEYIEPQFDNPLFIESCADQI
                     ARSFYEIPDEERSDTKIIFTAHSIPVPMAEDSPYVQQLETSAKLIAEKVEHEDWLLCY
                     QSRSGRPSDPWLEPDVCDVIDELAEQEKKIKHVIIQPIGFICDHVEVMYDIGIEAFQV
                     CRHHGIKMHRANTVNDDPKFLKGLANSVIKLIESS"
     gene            1360..>2152
                     /gene="hemG"
                     /locus_tag="AC159U_05945"
     CDS             1360..>2152
                     /gene="hemG"
                     /locus_tag="AC159U_05945"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR3336634.1"
                     /translation="MKIIIIGGGITGLAAAHRLIELNKENDLNIKIELLESSTRLGGN
                     ISTLKKDGLLIEEGPDSFITSKPYALNLCRRLGLEKDIISTSENNRRTLIYLNKRLVE
                     LPEGFILLSPTKILPFITSPILSLMGKLRALMELVIPKSSGNEDESLESFVTRRFGKE
                     MFENIAQPLIGGIYTSDPYKLSIKSAMPNIYNLEHKYGSIIKGLINQYGGQKESGARY
                     SQFVTLKNGMQTLVDSIISKIPKNSIITNKTVHKIKKTKKRWKVET"
BASE COUNT          722 a          398 c          473 g          559 t
ORIGIN      
        1 gattggttgc ttgtcaccgg aggactatag aacttatgta tttccgcata tgaaaacgct
       61 gtttagcagt attgataaaa acatacctac tgttcacttt ggtaccggca cgtctagttt
      121 gctcggacta atgaaacagg ccggagggac agtgattgga tttgactggc gtgtggatat
      181 caataaggca tggaaagaaa tagggtatac ttgctctgtg cagggcaata ttgatcctat
      241 agtgctgttt tcaacacctt caattattag aaaaaaagtt aaagaattac taaagaaagt
      301 taacggcaga aggggtcata tattcaattt gggtcatgga gtgctgcctg gtacgccacc
      361 agataatgtg ttggctctta ttgacgcagt gcacgaatat tcaagcagat aaggagaaat
      421 atgggaaagg attttaattc gattttaatg attggctacg gcgggccgga taaaccagaa
      481 gacataatgc catttttaga aaacgttgct aaggggttca acattcccag aagcagattg
      541 gaggatgtcg ctcatcacta tgagcttata gggggcaaat ctcccctaaa cgaattaacc
      601 tacaggcagg caataggtct tgaacatcag cttgctgcta tggggcatga cctccccgtg
      661 tttataggaa tgagaaactg ggaccccttt atacctgaca cccttaggat aatgaagagg
      721 gagcagaccc ttaactcagt ggggataatt cttgctgccc acaagtgcga tgcaagctgg
      781 gaaaaatata tgagagatat agaacaggca aaagaagata ctgacatcga tctgaatatt
      841 gaatacattg agccccagtt tgataacccg ctttttattg agagctgtgc cgatcaaatt
      901 gccagaagct tttatgagat accagatgaa gaacggtcag acacaaaaat tatttttacg
      961 gctcacagca tacccgttcc catggcagag gactccccat atgtgcagca gctggagact
     1021 agtgctaaac taatcgcaga aaaagttgaa catgaagact ggctgctatg ctatcaaagc
     1081 agaagcggca ggccatcaga cccatggctt gagcccgatg tgtgtgatgt gatagatgaa
     1141 cttgccgagc aggaaaagaa aattaagcat gtgattattc agccaatagg atttatatgt
     1201 gaccatgtag aagtgatgta tgatattggt atcgaagcat tccaggtctg ccgccatcac
     1261 ggcataaaga tgcacagggc aaacactgta aatgacgacc ccaagttttt aaaaggtctt
     1321 gcaaattcag taatcaaatt aatcgaatca tcttgatata tgaaaatcat tattattggc
     1381 ggcgggataa caggtcttgc agctgcacac agattaattg aattaaataa agaaaatgat
     1441 cttaatataa aaattgaatt actagaaagc tctaccagac ttggcggtaa tatttccaca
     1501 cttaaaaaag atggcctgct catagaggaa ggacctgact cattcattac ctcaaagccg
     1561 tatgctctga acctttgtag aaggcttgga cttgaaaaag atatcatatc cacatccgaa
     1621 aataaccgaa gaacgcttat atacctaaac aaaagactgg ttgagcttcc tgaaggcttt
     1681 attcttttat caccaaccaa aatattacct tttataacct cccctattct aagccttatg
     1741 ggaaagctaa gagcgctaat ggaattggtt atccctaaat catctggaaa cgaggacgag
     1801 agtcttgagt catttgtcac caggagattt ggaaaggaga tgtttgaaaa tatcgcccag
     1861 ccgcttattg gaggaatata tacttctgat ccgtacaagc ttagcattaa gtctgccatg
     1921 ccaaatatat ataatctcga gcataaatac ggaagtataa taaaggggct tataaatcaa
     1981 tatggagggc agaaggaaag cggagcaagg tatagccagt ttgttacact aaaaaatgga
     2041 atgcagacac tcgttgattc aattatttct aaaattccaa aaaactctat cattaccaat
     2101 aagacagttc ataaaatcaa gaaaacgaaa aaaagatgga aggttgagac tg
//
LOCUS       DBNGSV010000166         4057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_860569, whole genome shotgun sequence.
ACCESSION   DBNGSV010000166 DBNGSV010000000
VERSION     DBNGSV010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_860569"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1706
                     /locus_tag="AC159U_05950"
     CDS             345..1706
                     /locus_tag="AC159U_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3336635.1"
                     /translation="MPSKIRFFVLSLLLAPLLFVSNAAAETNSEVEQLKKQILEIQRQ
                     NQKQIEMLQQKIESMETKRASSDLKVEEFIAKKESEDEDAWWKGIRAGYKKGFFMESA
                     DGNFKTRFRTVGQFLMQVEDEDGGDTVTEFDIQRLRLIWDGNAFAPWFKYKVQLDIRE
                     DSINLRDLIFDFAYNPLFVPRVGQYKVPFNKEQLVSAFAQQTVNRSILDRQFSPQRDT
                     GISIHGKHGGYFWYHVGVFQGEGRNSDEGDENAADSNILWAGRVMFNPLGTGVKHQQN
                     FVKQTTIQLGAAIMGLDSDIVGEGNTALADETDSRNFRSRGGDFAAIGATDMQMLSWA
                     FDVSLMHPRGNIEASYIGADYDPSGTSQGSVYDQGFRVQAGVFLIPKVLEVAARYAYV
                     DLDDDLGDTDSIWEITPAVSYFLGQSHRWKVVADYSFIQEEGVDGTEEDTNRFRLQLQ
                     AYF"
     gene            2030..2362
                     /locus_tag="AC159U_05955"
     CDS             2030..2362
                     /locus_tag="AC159U_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336636.1"
                     /translation="MSGNNQQPSNPMDKIIIVILLLIVCLIAGAAYLFVQNKPQQVAR
                     TTQQERQTALQGKDLLREQLRETLSELRNIANEDILIQSAGAQTDIRLSRDTILSTGI
                     KPGKRTLM"
     gene            2575..2748
                     /locus_tag="AC159U_05960"
     CDS             2575..2748
                     /locus_tag="AC159U_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336637.1"
                     /translation="MGDLEWDQSSRTYAVQLSVPVIRNGVPVGVITGGVALDWLADEL
                     DLESFKDIRTQLN"
     gene            3255..3617
                     /locus_tag="AC159U_05965"
     CDS             3255..3617
                     /locus_tag="AC159U_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336638.1"
                     /translation="MSKSILVVDDSNTELEHISSIIKSKGYEVVTASSGEEALNKANI
                     AKPDLIVLDVVMPGKDGYQTCRELKKEENTKDIKIVMVTSKSQKTDEYWGRKQGADDY
                     ITKPFEPDNLLSSISSLI"
     gene            3778..>4057
                     /locus_tag="AC159U_05970"
     CDS             3778..>4057
                     /locus_tag="AC159U_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336639.1"
                     /translation="MGKRILLVDDSPTELEFMKKAVGTGEYELMTASNGDEAIKKCIE
                     FKPDLILLDVVMPVKDGFSVCRELKKNPETKDIKIIIVTSKNKPLDQQW"
BASE COUNT         1419 a          640 c          785 g         1213 t
ORIGIN      
        1 taaagcaaaa gctatctgtt gttctgtaaa tcttgacttc ctcatggcaa aatacctcct
       61 gatttattac gttattttgc cagatttctc tacttttgac tggttcagtt tacggggtga
      121 aggtcaaatt gaatattttt gttatgcatt ttcctaataa attgcgggta attcaatctg
      181 ctaatttaat gttgcaagat ttataccaaa cggcagtttt atgcatatat taagtaaatc
      241 atgaggtatt cgctgtaaaa tggcacagaa attgcacact ttcatatatg ttcattctgt
      301 gttaagaata aacatatatt actatattta taggaggtat ggagatgcca agtaagatta
      361 gattttttgt tttatcatta ctattggctc ctttactatt tgtttcaaat gctgcagcag
      421 aaacaaacag tgaagtagaa cagctaaaaa agcagattct ggagattcaa agacagaatc
      481 agaagcagat tgaaatgctt cagcaaaaaa ttgagtcaat ggaaacaaaa agagcttcca
      541 gcgatctcaa ggtagaggag ttcattgcca agaaagaatc agaggacgag gatgcctggt
      601 ggaagggtat aagagccggg tataagaaag gattcttcat ggaatctgca gatggtaatt
      661 ttaagaccag attcagaaca gtgggtcagt tcttaatgca ggttgaagat gaagatggtg
      721 gagatacagt cacagaattt gacattcaga gactaaggtt gatctgggat ggtaatgcat
      781 ttgcaccatg gtttaaatat aaggtacagc ttgatataag agaagacagt atcaatttaa
      841 gagacctaat atttgatttt gcttacaatc ctctatttgt tccaagggtt ggacagtaca
      901 aggttccatt taacaaggaa cagttagtct cagcgtttgc acagcaaaca gtcaacagat
      961 caatcctcga cagacagttt agtccacagc gtgataccgg tattagtatt catggtaaac
     1021 acggcggata tttttggtat catgttggtg tgttccaggg tgaaggaaga aactctgacg
     1081 aaggtgatga aaacgcagct gattcaaata ttctctgggc aggtagggta atgtttaacc
     1141 cgcttggcac aggagttaaa catcagcaaa acttcgttaa gcaaaccaca attcagttag
     1201 gtgcagctat tatgggactt gattcagata tagttggaga aggaaacaca gcacttgctg
     1261 atgaaacaga cagcagaaac ttcagatcca gaggtggtga ttttgcagca atcggtgcta
     1321 ccgatatgca gatgctatcc tgggcatttg acgtttcatt gatgcatcca agaggaaata
     1381 ttgaagcttc atatataggc gctgattatg atccatcagg taccagccag ggttcagtat
     1441 atgaccaggg tttcagagta caggccggtg tatttctaat tccaaaggta ttagaggttg
     1501 cagcaagata tgcctatgtt gaccttgatg atgatcttgg cgacaccgac tcaatctggg
     1561 aaataacccc tgctgtaagt tatttcttgg gacagagtca cagatggaaa gtggttgctg
     1621 actactcatt tatacaggaa gaaggcgtag acggtactga agaggataca aatcgtttca
     1681 gattacaact tcaggcctat ttctaaaatt taaagaactc atctctcctt acacataagg
     1741 agaactaaaa aacctgctga tttaaaattt aaatcagcag gtttttttat ttattatata
     1801 tatttatttt atgttttaat aattatatta atatagaaac cacttcccta taattaatat
     1861 aacaacggta acaaattcct ttattaaata gtcaatattt taattaaaac taattaaaat
     1921 ggcatagcga ttgcataata taacaagtaa aatattagaa gaaaatatta ttgaaagtaa
     1981 aaaaggtata tacttttagt aaaagaaatt attttggagg aaaagaaaca tgagtggaaa
     2041 taatcagcaa ccatccaacc caatggataa gataattatt gtcatcttgc tgttaatagt
     2101 ttgcttgata gcaggtgcag catatttatt cgtacaaaac aaaccgcagc aagtcgcaag
     2161 aacaacccaa caggaaaggc aaactgcact acaaggtaag gaccttttaa gagaacagtt
     2221 acgtgaaaca ctatccgaac ttcgcaatat agcaaatgaa gatatcttga ttcagtctgc
     2281 aggcgctcaa acagacataa gactatccag agatacaata ctaagcacag ggataaagcc
     2341 tggaaagaga acactgatgt agtatttaag caagaaatta ccacaaacac accgtcacaa
     2401 ttactaaagt ccaaagttga tggagcaggc tcccgttgga ctgagttatt tgtaactgat
     2461 aatcagggcg caaatgttgc agcctatccg gcaacatctg actactggca gggtgacgaa
     2521 gataaattta cagatgtact ggatatacag ggaaatcaaa ttaactcatt ttggatgggt
     2581 gaccttgagt gggatcaatc ttcccgtaca tacgctgtac agctttctgt cccagtaata
     2641 agaaacggag tgcccgtagg agtaataacc ggtggtgttg cgctggactg gcttgctgat
     2701 gagcttgatc ttgaatcatt taaagacatt cgtactcagt taaattaaat atctaataac
     2761 agattatttg atgatagaac aaataaaggc cttataacaa tataaggcct ttattttatt
     2821 aactgtttaa tacattctat ttattactta ttctacaatt aagttctttt tttccattgt
     2881 cttttccaga cttataggaa atcagttctt aacaaaaagg taaatcagtc cctgatacac
     2941 ctttatattt tcggatataa aattagacta ataataagaa tataaactaa gttgcataat
     3001 ataagagatt gctgagctat ctcttaaatt tgcaatcatt attgtaaata ttcattatta
     3061 ttacatttta taagattgaa gagcctctcc gtctgaacac tttaggttaa atttttatgg
     3121 aatttaaatt attatcaatt ttaaaaattc aggaaccaat aaatagttgt atattcataa
     3181 ctggcattta aattgcatgt attaaacaat aaagagtaaa gttatataag tcatataata
     3241 attggagtaa aaaaatgtca aaatcaatat tagttgtaga tgacagtaat acagaacttg
     3301 aacacatttc gagtattatt aagtctaaag gctatgaagt agttactgct tcatctggag
     3361 aagaggctct aaacaaggct aatattgcta aaccggattt gatagtttta gatgttgtta
     3421 tgcctggtaa agatggttat cagacttgca gagagctcaa gaaagaagaa aatacaaaag
     3481 atattaaaat cgtcatggtg acatccaaga gccagaaaac tgatgaatat tggggtagaa
     3541 aacaaggcgc agatgactat attacaaagc catttgagcc tgataacctg cttagcagca
     3601 tatcctcact catttaaatc tcactaatat aagctttttg catacttggg gtcatgcaca
     3661 tacaggctat gaccccctgt acattcgtac aggaaaaaac tgtacaatct atcgttttgc
     3721 tatttttttt atagtgttat attatatcct attaaaactt tagtatagga gctaggtatg
     3781 ggaaaacgca ttttattagt agacgatagt cctacagaat tagaatttat gaaaaaagcc
     3841 gtaggaactg gtgaatacga actaatgact gcttcaaacg gtgatgaagc tataaaaaaa
     3901 tgtatcgaat tcaaacctga tctaatatta cttgatgttg taatgcctgt taaagacgga
     3961 tttagcgtat gcagagaact aaaaaagaat cctgaaacaa aagatattaa gatcattatt
     4021 gttacaagta agaataaacc cctcgatcag cagtggt
//
LOCUS       DBNGSV010000167         1492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_167662, whole genome shotgun sequence.
ACCESSION   DBNGSV010000167 DBNGSV010000000
VERSION     DBNGSV010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_167662"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC159U_05975"
     CDS             <1..552
                     /locus_tag="AC159U_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR3336640.1"
                     /translation="NLSADIKNVVSPAVLWITFLFSGSIGLGRSFAMEKESEAIKGLL
                     LAPVDRSLIYVGKFLSNFTFLLIIELFTIPIFIVVFNYGLNINFMGIILVIFLGTVGF
                     GSVGTVFAAMSINTRLREVLLPILYFPIIIPVLLASVNITGSLLDGKMLTDNASSLQL
                     LIVFDIIFLSAGILLYQYVIEES"
     gene            772..1200
                     /locus_tag="AC159U_05980"
     CDS             772..1200
                     /locus_tag="AC159U_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336641.1"
                     /translation="MEYIFVLIIVLCVTAYICYPFIIGNTNTVFFNENIKGYTEAKKS
                     KLEILEDDKLELYSAIKEIDFDYGMGKLSEQDYKELRNEYLHKASLILKEIEKHSVQG
                     TEEAKDRLEEEIMRAKHEIRTAEDEIEIEIKKKREKKDLT"
     gene            1197..>1492
                     /locus_tag="AC159U_05985"
     CDS             1197..>1492
                     /locus_tag="AC159U_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336642.1"
                     /translation="MINTLQQHLWTFLTGIEKTSKRTGVVMVLIGWLVNNIKKLTSPS
                     NIFYRNPTHCRISGFIVPVLLITFIFSATASSQQLTGKELFVGNRCVNCHTLGRG"
BASE COUNT          495 a          234 c          291 g          472 t
ORIGIN      
        1 aatttatctg ctgatattaa gaatgtcgta agtcctgcgg tattatggat cacttttcta
       61 ttttctgggt caattggtct tggcagatca tttgccatgg aaaaagaatc agaagctatc
      121 aaaggccttt tattggctcc ggttgacagg agtcttatct atgtcggaaa atttcttagt
      181 aatttcacat ttttgctgat tatcgaactt tttaccatac ctatattcat agttgtgttt
      241 aactatggcc ttaacataaa tttcatggga atcattttgg ttatctttct gggcacggta
      301 ggttttggat ctgtaggcac agtttttgcg gccatgtcca ttaatacgag gctgagagaa
      361 gtgctgctcc caatactata ttttccaata attattccgg ttttactggc gtcggtaaat
      421 attacgggct ctcttctgga cgggaaaatg cttacagata atgcgtcttc attgcagttg
      481 ttaatagtct tcgatatcat ttttctctca gcaggaatac tactctatca gtatgtaatt
      541 gaagaaagtt aatgataata tttcagaatt ttgcattata taacaaatta taattcaact
      601 acttagacga attgtaattt gtttcaatca gtgttaagag caattacggc taagtcataa
      661 atattaaatc aatcgatttt gtaatgatga gtaaatattt attgcagtag caatttcaga
      721 atataaaggt aaaatcccac aactaaattc atctgttcca aacaataaac tatggaatat
      781 atatttgtat taataattgt tttgtgcgta accgcatata tttgctatcc gtttattatc
      841 ggcaatacca acactgtttt ttttaatgaa aatataaaag gctatacaga agccaagaaa
      901 agcaaactag aaatactgga agacgataag ctggagcttt acagcgctat taaagaaata
      961 gactttgact acgggatggg aaaactttcg gagcaagact ataaggagct taggaatgaa
     1021 tatcttcata aggcttcttt aatattgaag gagatagaaa agcactcagt tcagggcaca
     1081 gaggaggcaa aagacaggct tgaagaagag atcatgaggg ctaagcacga aatcagaact
     1141 gcagaagatg aaatagagat tgagattaaa aagaagaggg agaaaaaaga tttaacttga
     1201 taaatacctt acaacaacat ctatggactt ttttaacggg tattgagaaa acatcaaaga
     1261 ggacaggagt tgtaatggtt ctaattggct ggcttgttaa taacataaag aaattaacct
     1321 caccttcgaa cattttttat agaaatccga cacactgccg gatatctgga ttcatagtac
     1381 ctgttctttt aattacattt atattttcgg caacggcaag ttcacagcaa ctgaccggaa
     1441 aggagttatt tgtgggcaac agatgcgtca actgtcacac attagggcgc gg
//
LOCUS       DBNGSV010000168         9382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_98990, whole genome shotgun sequence.
ACCESSION   DBNGSV010000168 DBNGSV010000000
VERSION     DBNGSV010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_98990"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="dtd"
                     /locus_tag="AC159U_05990"
     CDS             complement(<1..262)
                     /gene="dtd"
                     /locus_tag="AC159U_05990"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3336643.1"
                     /translation="MKIVIQRVKQAKVKVNSEIIGNIQQGLLILVGAENDDDSSDIQY
                     LVSKVLNLRIFEDENSKMNLSVLDIKGEILAVSQFTLLGDCTK"
     gene            367..699
                     /locus_tag="AC159U_05995"
     CDS             367..699
                     /locus_tag="AC159U_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3336644.1"
                     /translation="MKSYIYGLIISSFLFTGVSFAGSYDITTGPGQMPSKIVKEHIVQ
                     HGEELHLLSAYYQLNPREWREIYNKNDYQISNPNLIYPKQKFKVSVYEDWEPPYDLDQ
                     WLKDNPRW"
     gene            complement(777..1712)
                     /locus_tag="AC159U_06000"
     CDS             complement(777..1712)
                     /locus_tag="AC159U_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014273.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3336645.1"
                     /translation="MIVKEIEISDIDISNPKYLILYNNISNELFNSIKSIGIINPPII
                     VKSDNKYIVLTGFKRFSVCKELGLKTVLCKVIDENEISFDKCIKIIYEDNRDRFTDIE
                     IGQLCKKYIYHGNDVKKFLELIGYPTSEKNIQRFTYLSNLNERILEYYIEERLNPEQC
                     FLLSQINTENALLILNKVLLPFRFNTNETREFLTELNDISIRDDISIAHIIENTLKSQ
                     EKINKNDIRLCIKKLRSPKLIEVEKEYHKIIKSLKLPKSVQISYSPYFESNYIEVKAK
                     IDGKDKLNELKTFIGNEDSFKRLIELISLVRGERN"
     gene            complement(1731..2669)
                     /locus_tag="AC159U_06005"
     CDS             complement(1731..2669)
                     /locus_tag="AC159U_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3336646.1"
                     /translation="MFVFEFVGDLPFFENQNRFQVYFKNISELRVGNPVKFSGLEVGK
                     VTDIKIDKGRIAVTFKADKDVPIREDSKATIRLTSLLGTSYLNVDYGTQSAKLAAAGS
                     TLVSEEPADINEILSNVQNTISSISSTFETFDIFGENKEDVSSLIKNLNTVVKNLSEG
                     KGTLGKLINEDKLYNDATSTFANVSDITSSIKSGKGTLGKLLTDESLYNETRTAMTNL
                     GEFTENISLEGGSLGKLLNDDTLYNEATEAATNLNAILKKINSGQGTLGLLVNDDSLY
                     LDAKDTLQKVDKGVDTVEDLAPLGIVGTVFGVVRFF"
     gene            complement(2740..3525)
                     /locus_tag="AC159U_06010"
     CDS             complement(2740..3525)
                     /locus_tag="AC159U_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3336647.1"
                     /translation="MEAQRKQNISSNDILSYLSGKAVSVTVSNVCKSFGSNQVLKNLS
                     LEVRPSETLVILGKSGSGKSVLLKHIVGLLQPDSGNIYINGDNITEIAGRKKHSIAMV
                     FQSSALLNSLTVEENVALYLKEHNIIKDDNDIAALVSSCLSIVGLQGKENVMPSELSG
                     GMKKRVAVARALITNPDLILFDEPTAGLDPMLVGNIAQLVNSLKTNIKVTQILVTHNV
                     SLASKIADRVALLYDGRIFKVSSFEEMRLSTEPEIRQFFDSSM"
     gene            complement(3529..4287)
                     /locus_tag="AC159U_06015"
     CDS             complement(3529..4287)
                     /locus_tag="AC159U_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3336648.1"
                     /translation="MNSLFNTTGGIINLFIESLYWSKDFMANRDKIYFQIINIGNNTI
                     PVAALISLFIGGVLALQTGPQLAQFGVEESIGGIVGLSMVKELGPVMAAILIAGRIGS
                     AMTAEISSMTVYEEVDALKTMDINPVKYLVMPRLLAVIIAMPILVVFMDLVGWFGGAV
                     VSAVNQDINVSYPIYFKNLKELVDFVAILNGMIKSVIFGILIISICGYVGLSTRGGPR
                     EIGTSVTRAVVLSFITVLISDYFITRFLFLIGLD"
     gene            complement(4329..5660)
                     /locus_tag="AC159U_06020"
     CDS             complement(4329..5660)
                     /locus_tag="AC159U_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336649.1"
                     /translation="MFDYTNNPELNKKHITALGPFFIYKTEDHKKQYGFRPLFYNEYD
                     YEKDKHYLDIAYPFAGYRRHENNRAFNIFFFINYNSTDRKNDIQRKFSIFPLFFSSFS
                     EDKEDNYLALFPLYGNIKNKFHKERIKFVLFPLFLQTESEGEINNNILWPFIGYQKGQ
                     DVEGFKFWPFYGYKKKKKSYYDEKFVLWPFYVKREKQFYDERVRYVSYLPFYSELDSK
                     NINQKSYLWPLFNKYTDRKNGFERIDAPWPVFNFTRGEKYENRFFPFFANEVSGEDKD
                     GYFLWPLYSYRVTDLEDYRISKKYYMLFLYKDVMEIPKNLNGRTSRRIDLWPIFSYHR
                     DRKGNRKINILSILSPFLPDNRQIENNFSPFWTLYEKKKMVTGEETTTILWNTYKSRK
                     SDDSRQVKISPLLPLFEYNRHDNIQVYKFFGGLIDIKRIKFISKTEFALLN"
     gene            complement(5773..6858)
                     /gene="alr"
                     /locus_tag="AC159U_06025"
     CDS             complement(5773..6858)
                     /gene="alr"
                     /locus_tag="AC159U_06025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3336650.1"
                     /translation="MRPTWAEINLKALSQNFLRTAELVGEKVKIMAIVKADGYGHGAV
                     RVSQTLQEQECHMFGVATVDEGLELRNSGINTPIIILSGICSDEIEIVVKNKLIPVVF
                     SLDLLKQLDKYGYKNNMKIKYHLKIDTGMNRLGLQSEKLNEYLSDLLSLNNVIMEGVF
                     THLSSADCDEAYTSNQILIFKQICDEFNKKGIKPIYYHSANSAALQRFKDSHFNLVRP
                     GIMIYGASAADDIYLHPVMKLKSKIIQIKELPKGARVSYGGTFITERVTKIAVLPIGY
                     ADGYSRHLSNKGVVSVNGFTAPVIGRVCMDLTIIDITNLKDINVGDEAVLFGDENISV
                     DYIASLADTISYELLSIVGKRVRRVYL"
     gene            complement(6883..8409)
                     /locus_tag="AC159U_06030"
     CDS             complement(6883..8409)
                     /locus_tag="AC159U_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3336651.1"
                     /translation="MEILKKSQIIDFDSLDKEIFVNTLSRSQELAKLIDKGSRLLPNY
                     YNFVFDLFLCFYKAEMLYNKKFEQGKGVNLFKTFIDDIYKNEFLKYIRNDTFLDETKS
                     AVATVTFGNSVLEWLKTNNFFSKKSFSDLNKIENIEEEIKEINSELEVIKDIQEKNND
                     EDLAKKLDSIENEKNIDIHRLQDEMDDSLNNMNKKITDNKDQITNFINTGLMDMDARM
                     SDYYSNSTLLEDNINSSANSEAGQKIDLAHKLIENDKLRKLANMMGSLRDIMHSVRNK
                     NWRTRTDEIHSITLGNDIGKAISSEIIKLSNDALKKDFFLKYLDGQLQQYHLKDNRSK
                     GPFIICLDCSSSMSGEKEIWSKGVALTLAETARKQKRKCDILAFTSGDYEVKHFDLSN
                     SSIKSIDSEQFISVAEYFPGGGTSFEKPLTKALDLLKEYRENNADIVFITDGESHIGP
                     GWLESFKEDKKNYEFKVYSILIDLVGNESQRTLEMFSDKITNVKNLTADNSKKIFVDI
                     "
     gene            8500..>9382
                     /locus_tag="AC159U_06035"
     CDS             8500..>9382
                     /locus_tag="AC159U_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3336652.1"
                     /translation="MSLNKQEKKAVLSVSVIISIRLLGIFLILPTFSIYVLEYPLATK
                     ITAGIAFGIYAISQSLLQIPFGWASDKFGRKRVLIFGILIFIIGSVLCGFADNINELI
                     LARVLQGSGAISSVAISSLGDLTRPEVRAQSFTITGIMIGVTFIVGMLLGPLLAAKLG
                     FESLFFILAVLGVIAIAATLLYFPDIEVKKLEINKTRLYAYLKNKDIKTLFFSAFINS
                     ITTNIFLFVFPLTWVLLGESSENIWEAYLIILVPSFLVVFPYIRRAEKNKNLKISTKA
                     AWLLLLICFVSYLFVESD"
BASE COUNT         2914 a         1729 c         1344 g         3395 t
ORIGIN      
        1 cctttgtgca gtctccaaga agcgtaaact gagaaactgc taaaatctcg ccttttatgt
       61 ctaatacaga aagattcatt ttagaatttt catcctcaaa tatccttaaa tttagtactt
      121 tagataccaa atattgtata tccgagctat catcatcatt ctctgcaccc actaaaatca
      181 acagcccctg ttgaatattt ccaattatct cactattgac tttaactttt gcttgtttta
      241 ctctttgtat tactattttc ataacttaaa gtagtttttt tagaaaactt aataaattaa
      301 tttaacattt tgaaaaaatg tagtaaaata attacatatt ttaaataagc ggaggatatt
      361 caaataatga aatcttatat atacggttta ataatatcta gtttcctttt cacaggtgta
      421 tcatttgctg gttcttatga tattaccaca ggcccggggc agatgcctag caaaattgta
      481 aaagaacata tagttcagca cggtgaagaa cttcatttac tttccgctta ttatcagcta
      541 aacccaaggg agtggagaga aatttataac aagaatgatt atcagattag taacccaaac
      601 ttaatatatc ctaaacagaa atttaaagtt agcgtatatg aagactggga gccaccatat
      661 gaccttgatc agtggctaaa agataaccct agatggtaat ctagtttagt atataaaaac
      721 aacaacaaaa aaaaggtgac tattatagtc accttttttt atacctaatt ttagatctaa
      781 tttcgttctc ccctcactaa gcttattaat tctatcaatc tcttaaagct atcttcattg
      841 ccgataaagg tttttaattc atttagctta tcctttccat ctatttttgc ttttacctca
      901 atgtagttac tttcaaaata tggtgaatag ctgatttgaa cacttttggg aagcttaaga
      961 ctcttaataa ttttatgata ttctttttca acttcaataa gttttggact ccttaatttc
     1021 ttaatacaaa gcctgatatc gtttttatta atcttttcct gactttttag tgtgttttca
     1081 ataatatgag caatagatat gtcgtctctt atagaaatat cattcaattc agttaagaac
     1141 tctcttgttt catttgtgtt aaacctgaaa ggaagaagaa ctttgtttaa aatcagtaaa
     1201 gcattttctg tattgatttg ggaaagtaaa aaacattgct caggatttaa cctctcttct
     1261 atataatatt ctaagattct ctcattcaaa ttcgataaat acgtaaatct ttgaatattc
     1321 ttttcagagg taggataacc aattaattcc aggaattttt taacatcatt tccgtgataa
     1381 atatattttt tacaaagttg ccctatttca atatccgtaa atctatctct attgtcttca
     1441 taaattattt taatacattt atcaaaagaa atttcatttt catctattac tttacaaagt
     1501 acagttttta atcccagttc tttgcaaaca ctaaaacgtt taaaaccagt caggacaatg
     1561 tatttattat cgcttttaac gataattggg gggttaataa ttccaatgct ttttatggaa
     1621 ttaaataact catttgaaat attgttgtac aaaattaaat atttgggatt agaaatatct
     1681 atatctgata tctctatttc tttaactatc atttagaata aatgtgttaa ttagaaaaat
     1741 ctaactactc caaatacagt tcctactatt ccaagtgggg ctaagtcctc tactgtatca
     1801 acacccttat ctaccttttg caacgtatcc ttagcgtcca aataaagtga gtcatcattt
     1861 acaagaagcc caagagttcc ctgcccacta ttaatttttt taagaattgc atttaagtta
     1921 gtagcagctt cagttgcttc attataaagg gtgtcatcat ttaaaagttt tcccaaagac
     1981 ccaccctcaa gactaatgtt ttcagtaaat tctccaagat ttgtcatagc agttcttgtt
     2041 tcattgtata aagactcatc tgtaagtaat tttcccaatg tgccctttcc acttttaatt
     2101 gaacttgtta tatcgcttac gttagcaaat gtacttgtcg catcattata gagcttatct
     2161 tcgttaataa gcttacctaa tgtgccctta ccttctgaga gattttttac aacagtattt
     2221 aggtttttta taagactaga aacatcttct ttattttctc caaatatatc aaaggtttca
     2281 aaagtggagc ttattgagct tatagtattt tgaacatttg ataatatctc atttatatcc
     2341 gcaggttctt cgcttacaag tgtagatccg gcagcagcta gcttagcact ttgagttcca
     2401 tagtccacat taaggtagct tgtacccaaa aggcttgtaa gcctgatagt agcctttgaa
     2461 tcttctctta taggtacatc tttatccgct ttaaatgtga cagcaatcct tcccttatct
     2521 atttttatat cggttacttt acctacctca agtccagaaa atttaacagg atttcccact
     2581 ctaagctcgc tgatattctt aaaatagact tgaaaacggt tttgattttc aaaaaaagga
     2641 aggtctccta caaactcaaa gacaaacaac agcaataaga atccaactaa gaaaaataaa
     2701 cctgcccttg tattgttatt tatttacaac ctcctttatt cacatacttg aatcaaagaa
     2761 ctgccttatt tcaggctcag tactaagtct catttcttca aacgaactga ctttaaatat
     2821 tcttccatca taaagtaaag ctactctgtc tgctatttta gacgccagac ttacattatg
     2881 agtaactaat atctgagtaa cttttatatt tgttttcagg ctgtttacaa gttgggcaat
     2941 atttcctacc agcataggat caagccccgc tgtaggctca tcaaacagta ttaagtcagg
     3001 attagtaatt aacgcccttg caacagctac cctttttttc attccaccac taagctccga
     3061 aggcattaca ttttcctttc cttgtagtcc aactatagat agacatgatg aaactaaggc
     3121 ggctatatca ttatcatctt taataatatt gtgctcctta aggtagagcg caacattttc
     3181 ttctactgta agcgaattaa gcagagctga tgactggaat accatagcaa tactatgttt
     3241 ttttctgcct gcaatttctg taatgttgtc tccgtttata tagatatttc cactatcagg
     3301 ctgtagtaag ccaacaatat gttttagcag tacacttttc ccagaaccgc ttttaccaag
     3361 tattacgagg gtttctgatg gcctgacttc tagggataag ttttttaata cctgattgct
     3421 gccaaaagat ttacaaacat tagatacagt gacacttacg gcttttccac ttaagtagga
     3481 aagtatatca ttactactaa tattttgttt tctttgtgct tccatatttt agtccagccc
     3541 aatgagaaat aaaaaccttg tgataaagta gtcagaaatt aatactgtaa taaaagacag
     3601 tacaacagcc cttgtaacag aggttccaat ctctcttggc cctcccctgg tactaagtcc
     3661 cacataaccg catatggata taattaaaat tccaaagatt actgatttaa tcattccatt
     3721 tagtatggca acaaaatcta caagctcttt taaattcttg aaatatatag gataagaaac
     3781 atttatgtcc tggtttacag cagatactac agctcctcca aaccagccaa ctagatccat
     3841 aaatacgacc aatattggca tcgctataat gaccgccagc agtcttggca ttacaagata
     3901 ctttacagga ttaatatcca tggtttttaa ggcgtctact tcttcgtata cagtcataga
     3961 gcttatctca gctgtcatcg cagagcctat tcttccggca attaaaatag ccgccatcac
     4021 cgggccaagc tcctttacca tggataggcc aacaatacct ccaatacttt cttctacacc
     4081 aaattgagca agttggggac cagtttgtaa ggcaagcaca ccaccaataa aaagagatat
     4141 gagagcagct actggtattg tattatttcc aatattaatt atctgaaagt atattttatc
     4201 tctattggcc ataaagtctt ttgaccagta taaactctca ataaataaat ttattattcc
     4261 acccgttgta ttaaacaggg aattcatgcc ttttatcata ttactatcat gtatatcata
     4321 agttattgct aatttaaaag cgcaaattca gtcttactta taaattttat tcttttaatg
     4381 tctatcaaac ctccaaagaa tttatagact tgaatgttat catgtctatt gtattcaaat
     4441 aaaggtaaaa gcgggcttat cttaacctgc ctgctgtcat ccgatttcct gcttttataa
     4501 gtattccata atatagtagt tgtttcttct cctgttacca tttttttctt ctcataaaga
     4561 gtccaaaaag gcgaaaagtt gttctctatt tgtctgttat ccggcaaaaa ggggcttagt
     4621 attgataaaa tatttatttt cctgtttcct tttctgtccc tgtgatatga aaatattggc
     4681 cacaggtcaa ttcttctaga tgttctgccg tttaagtttt ttggtatttc cattacatcc
     4741 ttataaagaa aaagcatata atattttttt gatatcctat aatcctcaag atccgtgact
     4801 ctatagctat acaaaggcca gagaaaatat ccgtctttgt cttctcctga aacttcattt
     4861 gcaaaaaacg ggaaaaacct gttttcgtat ttttcccctc tggtaaagtt aaaaaccggc
     4921 catggggcat cgattctttc aaatccgttt ttcctgtctg tatatttatt gaataacggc
     4981 cagaggtatg atttctggtt tatgtttttt gagtcaagct ctgagtagaa cggaaggtat
     5041 gatacatatc ttactctctc atcataaaac tgtttttctc tttttacata aaaaggccat
     5101 aatacgaatt tttcatcgta ataagatttt ttctttttct tatacccata aaaaggccag
     5161 aatttaaaac cctcaacatc ttgccctttt tgatagccga taaaaggcca caatatatta
     5221 ttgtttatct ccccttcact ctcagtctgt aaaaacaagg gaaacagcac aaatttaatc
     5281 ctttccttgt gaaatttatt cttaatattt ccgtatagag ggaaaagcgc taaatagtta
     5341 tcttccttat cttcagaaaa gctagaaaag aaaagcggga aaatactaaa ttttctttgt
     5401 atatcgttct ttctatcagt ggagttataa tttataaaga agaatatatt gaaagctctg
     5461 ttattctcat gccttctgta tcctgcaaat ggataagcta tatccagata gtgtttatct
     5521 ttttcataat catattcatt gtagaacagg ggtctgaatc catattgttt tttatgatct
     5581 tctgttttat agataaagaa agggccgaga gcagtaatat gctttttgtt aagctcaggg
     5641 ttgttcgtat agtcaaacaa aggaccaaaa cttatttctt ccgcacttat agatagggag
     5701 gtaataatca aaaatatagc aatatatata aaatgcttaa aacgattcaa tataaattcc
     5761 cccacaccta aactataaat ataccctccg aactcttttt cctacaattg agagtagttc
     5821 gtacgatatc gtgtctgcaa gtgatgcaat ataatctacg ctgatatttt catctccaaa
     5881 caatactgct tcatcaccaa catttatatc tttaagattt gtaatatcaa ttatagtaag
     5941 atccatacaa accctaccaa ttacaggagc tgtaaaaccg tttacgctta caaccccttt
     6001 atttgataag tgtctcgaat atccatctgc atatcctatt ggaagtacag caattttagt
     6061 taccctttca gtaataaatg ttcctccata gcttactctg gctcctttag gtagctcttt
     6121 aatctggata atcttagatt taagcttcat taccggatgt aaatatatat catccgctgc
     6181 acttgctcca tatatcatga ttccaggtct tacaagatta aaatgtgaat ctttaaacct
     6241 ttgtagtgct gcactattgg cactgtgata atatatcggc tttatccctt ttttattaaa
     6301 ttcatcacaa atctgcttaa atattaaaat ttggttacta gtatatgcct catcacagtc
     6361 tgcacttgaa agatgtgtaa agactccttc cattattaca ttatttagac ttaataaatc
     6421 agataaatat tcatttaatt tttcactttg tagtccaagc ctgttcatcc cagtatcaat
     6481 ttttagatga tacttaattt tcatattgtt tttataacca tatttgtcca gttgttttaa
     6541 aaggtctaat gaaaaaacta caggaattaa cttgtttttt acaactattt cgatctcatc
     6601 agaacaaata ccgcttagga taataattgg ggtatttatt ccagaatttc gtagttcgag
     6661 gccttcatcc acagtagcaa cgccaaacat gtggcattcc tgctcctgta gagtttggct
     6721 aactcttaca gcaccatggc cataaccatc tgctttaact atggccataa tcttaacttt
     6781 ttcgccaaca agttcggctg ttcttaaaaa gttttgagaa agagccttta aatttatctc
     6841 agcccatgta ggtcgcattg tatattgata atcggattag ggttaaatat caacaaatat
     6901 ttttttagaa ttatctgcag ttaaattctt aacatttgta attttatcac taaacatttc
     6961 tagtgtacgc tggctttcat ttcccaccag gtcaatcaat atcgagtaca ctttaaattc
     7021 atagttcttt ttatcctctt tgaatgattc aagccagcct ggtcctatat gtgactctcc
     7081 gtcagtaata aatacaatat cggcattatt ttctctatac tcttttagca aatcaagcgc
     7141 ttttgttaga ggtttttcaa aactcgtacc gccccctgga aaatattctg caacactaat
     7201 aaactgctca ctatcaatag atttaatact gctgtttgat aaatcaaaat gttttacttc
     7261 ataatctccc gaggtaaagg caagtatatc acactttctt ttctgttttc ttgcagtctc
     7321 ggcaagtgta agagctaccc cttttgacca tatttccttt tcaccactca ttgatgagct
     7381 gcagtctaga catataataa atggcccttt ggatctgtta tcttttaggt gatattgctg
     7441 tagctgtccg tctaaatatt ttaagaaaaa atctttttta agtgcatcat ttgaaagctt
     7501 tataatttca gaagaaattg ccttaccgat atcatttccc agagtgatag aatgtatttc
     7561 atcagttctg gttctccagt ttttgtttct aacagagtgc ataatatctc ttaagcttcc
     7621 catcatattt gcaagttttc taagtttatc gttttcaatt aatttatggg ccagatcaat
     7681 tttctgtcct gcctcagaat ttgctgaaga attaatatta tcttcaagaa gtgtgctatt
     7741 tgaatagtaa tcactcattc tagcgtccat atccataaga ccggtattaa taaaatttgt
     7801 tatctgatcc ttattgtcag tgattttctt attcatatta tttaatgaat catccatctc
     7861 atcttgaagt ctgtgaatat caatattctt ttcattttct atcgaatcca gcttttttgc
     7921 taaatcttca tcattatttt tctcttgaat atcttttatg acctcaagct cactattaat
     7981 ctccttaatt tcttcttcta tattctctat tttatttaga tcagaaaacg attttttgga
     8041 gaaaaaattg ttggttttaa gccactccag caccgaattt ccaaatgtaa cagttgctac
     8101 agcagatttt gtttcatcga gaaaggtatc gtttcttata tattttaaaa actcattttt
     8161 atatatatcg tctataaaag ttttaaatag atttactcct ttaccttgtt caaatttttt
     8221 gttgtatagc atttcagcct tataaaagca caggaacagg tcaaaaacaa aattgtaata
     8281 gtttggcaat aatctgctgc ctttatctat tagttttgca agctcttgtg atctggataa
     8341 agtatttaca aaaatttctt tgtccaatga atcaaaatcg attatttggc tcttttttaa
     8401 gatttccatt gaattttaag attaacatat aagataactg tatcaattta gttatctgat
     8461 gatagaataa tcagctattt tataagtcat taaaattaaa tgagcctaaa caaacaagaa
     8521 aaaaaagctg tactaagcgt aagcgtaatt atttcaatac gccttctggg tatattttta
     8581 atattaccta catttagtat atatgtgctt gaatatccgc ttgcaacaaa aatcactgct
     8641 ggcatagcct ttggtatata tgcaatatca cagagcctgc tgcagatacc ctttggatgg
     8701 gcaagtgaca aattcggcag aaaaagagta ttaatatttg gaatccttat atttattatt
     8761 ggaagtgtac tctgcggatt tgccgataat ataaacgagc ttatactggc tagggttctt
     8821 cagggaagcg gagccataag ctctgtagca atttcatctc ttggcgatct tacaagaccc
     8881 gaggtaagag ctcagtcttt tacaataaca ggaattatga ttggcgttac atttattgta
     8941 ggaatgctcc ttggaccgct gcttgcagca aagctgggtt ttgagagtct cttttttata
     9001 ttggctgttc ttggtgttat agcaattgct gcaacacttt tatatttccc tgatatagag
     9061 gtcaagaaac tagagataaa caagactcgt ctttatgcat atttaaagaa taaagacatt
     9121 aaaactctat ttttctccgc atttataaat tcgataacga caaatatttt cctgtttgta
     9181 tttcctctaa catgggttct gcttggagaa tctagtgaga atatatggga agcttattta
     9241 attattcttg ttccgagctt tcttgttgta tttccttata tcaggagggc tgaaaaaaat
     9301 aaaaacctta aaatatctac aaaagctgca tggctgttgc tgcttatttg ctttgtttct
     9361 tatctttttg tagaatccga ta
//
LOCUS       DBNGSV010000169         6166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_860827, whole genome shotgun sequence.
ACCESSION   DBNGSV010000169 DBNGSV010000000
VERSION     DBNGSV010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_860827"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1402
                     /gene="carA"
                     /locus_tag="AC159U_06040"
     CDS             290..1402
                     /gene="carA"
                     /locus_tag="AC159U_06040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR3336653.1"
                     /translation="MSKAVLLLEDGTHFFGRAFGSKGEVFGEIVFNTSLTGYQEILTD
                     PSYSEQIIVMTYPEIGNYGINDVDVESSKPHAKGFVVKEFWKTPSNWRSQGGLGTYLK
                     DHGIVGIEWIDTRAITRKIRNDGAQKCVISSVDLDIYSLKKKVHAYPSITGRDIVSPV
                     TCEKAYQWNEGTDDWNPKPDSIKTAGKHNVVVYDFGVKKNILRRLVDLGCDVSVVPST
                     TSPEDILSMNPDGILLSNGPGDPFPVEYAAENVKSLIGKKPIFGICLGHQVLSLALGA
                     KTFKLKFGHRGGNQPVKNLRTGEVEITSQNHGFAVAADSFGKDIEITHINLNDDTVEG
                     IEHKKHPAFSVQYHPEASPGPHDASYLFDKFIELMS"
     gene            complement(1417..1848)
                     /locus_tag="AC159U_06045"
     CDS             complement(1417..1848)
                     /locus_tag="AC159U_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336654.1"
                     /translation="MGPNPVGAVIGGFIGALSGAIVWTGVTIVLMQELGLAAIAIGFI
                     TALFVRKLGKGTTNIYGLIGAFFTFFGCILGKILTVIFHLSRETGISFLDTLMDFDLG
                     VILPMLEQTIQKFDYLFLAAAVFTGYLYSTHKGKQMHFRRR"
     gene            complement(1838..3439)
                     /locus_tag="AC159U_06050"
     CDS             complement(1838..3439)
                     /locus_tag="AC159U_06050"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HIR3336655.1"
                     /translation="MQSTSDIIKILIPAVGGQGGGVMTEWLVTAFLNSGFEVQSISLP
                     GLAQRGGSTAYYIEAYKADTTSSNSQLIFSQYPIPGDIDIILCQEFLELGRVLEQGYG
                     SKKTCIISSTHRIYSTPEKLPVSSGIFSDDKLHNLAIEFSSRFIGLDAKQIAKQNGMD
                     DLAINAILFGALGASKSIALRSDNLRTAIESVGIAVKNNLNAYEIGYDYAMNNLAGSD
                     KESAKSLKYLDEKKITTKRKYREDFIKISDYINNFFPENLHIYLIEAAHILIDYQDPE
                     YAEKYLEKVNKVINADKTHNLLISEQFSKNLALLMSYEDGIRVAELKVKSIRFSNIKE
                     EMKIEDSQIFNVTDYLKPDAYEIYGLMPDFIMSPVIKIIDNTPLKRLKRRQKDITFAQ
                     KPKTTSFFGFLRLFLLTKLKPLRPYSHRYANESRVINKYIKTTLDYSNKDQQLGLLAA
                     KSGSLVKGYGDVRRKTVDVFTRFIDNIIWPISKDLRDDPDNNKALINFAEQCLDTIGS
                     ESDKISEYEKEMREKILFQGVKNGP"
     gene            complement(3501..5618)
                     /locus_tag="AC159U_06055"
     CDS             complement(3501..5618)
                     /locus_tag="AC159U_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3336656.1"
                     /translation="MGSVYSNDLISQLNLGEGQTLYGDTPLTILKSFLQSGVSYVGGY
                     PGAPTASLIDVISDAYDPVLKDYGMYFDCSGNEASAAALLSASINYPIRGSVNWKIVG
                     TNVASDALAHICSSGVTGGAMIIVGEDYGCDGTIVAEKAMPFALKSSMAVIDPRGDLG
                     RISFLIEKGFELSEYSNLSVMFILSTRIAYIRNKMICKDNITPQISTLSPIDELIQDV
                     TKIPLPPYSQQQEKLKYESRIPAAKEFILNNNINELFIGDDSNIGIITHGSLYNSLLR
                     CLYNLGEAELTGKSSISILNLNVTYPLVPEQITEFISDKKSVLVLEEGMPNLIEEQIR
                     SIAQRNKIYSDIFGKDIMPVNGEYTPDRIIKGIGDFLVSNAQSPSKKQAISKKSLEVE
                     EHISKASSFLDEPVTLRNPIFCTGCPERPIFTAIKILEEKYGRSYYASDIGCYSMSGL
                     PPFNLSDSITGMGIGLASAGAISKMTDKRTISFMGDGTFWHSGLTTSVANALYNNQNA
                     ILIILENFWTSMTGHHENPTSGENMRKEGVAMSIEQTLRGAGVKWIKEVDPYDLSDSM
                     KVLSKAYEHDDGLRVIISRGECQLEKDRRERVIIKKDINEGKRTVQTKLGVDAEVCTG
                     DHSCMRHNGCPSLTLKQSPNILRKDPVAHIESSCVGCGLCGEIAHAAVLCPSFYKVDV
                     ITNPSMLDKLSKRVNNYLISLLN"
     gene            complement(5686..>6166)
                     /locus_tag="AC159U_06060"
     CDS             complement(5686..>6166)
                     /locus_tag="AC159U_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3336657.1"
                     /translation="KEISDLIEPIINNSNNQTVEFLFNLNNYIYQDFEKIIRVTGNPW
                     KPVKTLKENKGSCRDLAVLFIECCRSVGLPSRFTSGYNEIDIDTETRQLHAWAEVFIP
                     GAGWKGYDPTLGLAVGDKNISLSSSSLPQNTYPISGNFRGTGASSELQYKIEIYPIS"
BASE COUNT         1849 a         1181 c         1109 g         2027 t
ORIGIN      
        1 cattggttgc cagatcgcat tcgtatccta aagttgataa tatgtcatta agcagaaaac
       61 ggattaaatc aatgtcatct acaattagta ttttttcgtt gctcatataa aactaattac
      121 ctttgtaaca gatgagtaaa tatttaaaac aaataaaagt ttaaagctac taaactaata
      181 attatatact aaaataaaac aacaggaata ataatatata ataatcaata tatcatatca
      241 taacatgata tttgacacgg tataatttga tgttattttc aaacccgaaa tgagcaaagc
      301 agttctatta cttgaagatg gtacacactt ttttggccgc gcatttggct ctaaaggaga
      361 agtgtttgga gagatagttt ttaacacttc attgaccggt tatcaggaaa ttcttactga
      421 tccctcttat agtgaacaaa tcatagtaat gacttatcct gaaattggaa actacggcat
      481 aaatgacgtg gatgtagagt catcaaagcc ccatgcaaaa ggttttgtgg ttaaagagtt
      541 ttggaaaact cccagcaact ggaggtctca ggggggtctt ggtacatatc taaaagatca
      601 cggcattgtc ggaattgagt ggattgatac aagagctatt acaagaaaga taagaaacga
      661 tggggcgcaa aaatgtgtta tttcctctgt agacctcgat atttatagtc ttaagaaaaa
      721 agtacatgct tatccttcta ttaccggcag ggatatagtc tcaccggtaa cttgtgaaaa
      781 agcttatcaa tggaacgaag gtacggatga ttggaatcca aaacctgaca gcattaagac
      841 tgcaggaaaa cataatgtgg ttgtttatga ttttggcgta aagaaaaata ttctaagaag
      901 acttgttgat cttggatgtg atgtaagtgt ggttccatct actacatcac cagaggatat
      961 tttatccatg aatcccgacg gcatactgtt atcaaacgga cccggtgacc cctttccggt
     1021 agagtatgct gccgaaaacg tgaagtctct tattgggaaa aaaccgatat ttggaatttg
     1081 tctgggtcat caggttttaa gtcttgctct gggtgcaaag acatttaagc ttaaatttgg
     1141 ccaccgcgga gggaatcaac ctgttaagaa ccttaggaca ggtgaagttg aaataacatc
     1201 tcaaaatcat ggttttgccg ttgctgcgga ttcatttgga aaagatatag aaattacaca
     1261 tataaatcta aacgacgata ctgtagaagg tattgagcat aaaaaacacc ctgcattctc
     1321 agtgcagtat catcccgaag cctctcccgg gccgcatgat gcttcatatt tatttgataa
     1381 gtttatcgag cttatgtcat aaatgttttt acaaggtcac cttcttctaa agtgcatttg
     1441 ttttccctta tgagttgaat acaagtatcc cgtaaaaaca gcagctgcca ggaatagata
     1501 atcaaatttt tgtatggttt gttccagcat cggaagaata acacccaggt caaaatccat
     1561 aagtgtatca agaaatgata ttcccgtttc acgcgataag tgaaagatca cagtaagtat
     1621 ctttcctaaa atgcatccaa aaaaagtaaa aaatgcccca ataagtccat aaatgttggt
     1681 tgtgcctttg ccaagttttc ttacaaaaag agcggtaata aatcctatag caattgcggc
     1741 aaggcccaat tcctgcatca atactatcgt tactccagtc cacactatag ctcctgaaag
     1801 tgctccaata aatccgccta ttactgctcc aaccgggtta gggcccattt tttactcctt
     1861 gaaaaaggat tttttctctc atctcttttt catattcact aattttatca ctctcagaac
     1921 ctattgtgtc cagacactgc tctgcaaaat taatgagagc tttgttgtta tccggatcat
     1981 ctcttaagtc cttggagatt ggccagatta tattatctat aaatcttgta aatacatcaa
     2041 ctgtttttct tctcacatcc ccatagcctt taactaatga accacttttt gctgctaata
     2101 gtcccagttg ctggtcttta ttagaataat ccagtgtagt cttgatgtat ttatttatta
     2161 ccctgctctc atttgcatat ctatgtgaat atggtcttaa tggttttaat tttgttagta
     2221 ggaatagtct taaaaaccca aagaacgaag tagtctttgg tttttgtgca aatgtaatat
     2281 ctttttgcct tcttttcagt ctttttagtg gcgtattatc aataatttta ataacagggc
     2341 tcattataaa gtcaggcata agaccataaa tctcatatgc atctggttta aggtagtctg
     2401 taacattaaa aatctggcta tcctcaatct tcatctcttc tttgatattg gaaaatctta
     2461 tggattttac ttttagctca gccactctta ttccgtcttc atagctcata agaagcgcca
     2521 ggttttttga gaactgttct gatattaata aattatgtgt tttatcggca tttattactt
     2581 tattaacctt ttcaagatac ttctctgcat actcgggatc ctgataatct atgagtatat
     2641 gagcggcttc aatgagataa atatgcagat tttctgggaa aaaattgttt atataatctg
     2701 aaatctttat aaaatcttct ctatatttcc ttttggttgt aattttcttt tcgtctaaat
     2761 actttagaga tttagcagat tctttatcgc tgccagccaa attattcatt gcatagtcat
     2821 atccaatctc ataagcgttt aggttgtttt taacggctat gcctaccgat tcaattgcag
     2881 ttctaaggtt atcggaccgc agagcaatag attttgatgc acccagtgct ccaaacagta
     2941 tggcatttat agcaagatca tccataccgt tttgttttgc aatctgctta gcatccaggc
     3001 ctataaatct ggaagaaaat tcaattgcta agttatgaag tttgtcgtct gaaaatattc
     3061 cgctcgaaac aggaagtttt tcaggggttg aataaattct gtgtgtgctt gagatgatac
     3121 aggttttttt tgacccatac ccctgttcta aaacacgtcc aagctcaagg aactcctggc
     3181 acaatattat atcaatatct cctggtatgg gatactgtga aaagattaat tgtgaattgc
     3241 tgctggtagt atcagcttta tatgcttcaa tataatatgc ggtggaaccg cctctttgag
     3301 ctagccccgg aaggcttatg ctctgtactt caaagccgct gtttaagaat gctgtaacga
     3361 gccactcggt catcactcca ccgccctgac ctcctacagc tggaataaga atcttaataa
     3421 tatcactggt actttgcata tctaaatttg gattataaaa tgaaaagctg aattttaaag
     3481 aataattatt taggctattt ttaatttaaa agagatataa gatagttatt tactcttttt
     3541 gacaacttat caagcataga aggattggtt attacatcaa ctttataaaa tgacgggcaa
     3601 agcacggcag catgtgcaat ctctccgcaa aggccacatc ccacacaaga gctttcaata
     3661 tgggcaacag gatcttttct taaaatattt gggctttgtt taagtgtaag ggaagggcag
     3721 ccattgtgac gcatacatga atgatcaccg gtgcatactt cagcatcaac acccagtttg
     3781 gtctgtaccg ttctctttcc ctcattaata tcttttttaa ttattactct ttctcttctg
     3841 tctttttcaa gctggcactc tcccctggaa ataataaccc taaggccgtc atcatgttca
     3901 taagcctttg agaggacttt catggaatct gataaatcat acggatcaac ctcttttatc
     3961 catttaacac ctgcacctct tagggtctgc tctatgctca ttgcaacccc ttccttgcgc
     4021 atattctctc cagatgtagg gttttcatga tgcccggtca tggatgtcca aaagttttct
     4081 aagatgatta gaattgcatt ctgattatta tataaagcat ttgctacgct tgttgtaagg
     4141 ccgctgtgcc agaacgtgcc gtctcccata aaagagattg ttcttttatc ggtcatttta
     4201 gagattgcgc ctgcagatgc aagacctatt cccatccccg ttatagaatc actcaggtta
     4261 aaaggcggaa ggccgctcat tgaataacat ccaatgtctg atgcatagta tgacctgccg
     4321 tatttttctt ctaatatttt aattgcagta aatattggtc tttccggaca tcctgtacag
     4381 aaaataggat ttctaagtgt tacaggctcg tctaaaaatg aggatgcttt tgaaatgtgt
     4441 tcctctactt ctaagctttt tttacttatt gcctgttttt ttgaaggtga ctgtgcattg
     4501 gaaactagaa aatcacctat tccctttatt attctgtccg gggtgtattc gccatttact
     4561 ggcattatgt ccttgccaaa aatatctgaa taaattttat ttctctgcgc aattgatctt
     4621 atctgttctt caataagatt tggcattcct tcttctaaaa cgagtactga tttcttatca
     4681 gaaataaact cggtaatctg ctccggtaca agagggtagg tcacatttag atttagaata
     4741 gatatactgc tttttccggt aagttctgct tctccaaggt tataaagaca ccttaacagc
     4801 gaattatata gacttccgtg agtaatgatt ccgatattac tgtcatcacc tataaatagt
     4861 tcattgatat tgttatttaa aataaactct tttgcagccg gtattctgct ttcatattta
     4921 agtttttcct gctgctgaga gtagggggga aggggaatct ttgtcacatc ctgaattaac
     4981 tcatcaattg gactaagagt gcttatctga ggggttatat tatccttgca aatcatttta
     5041 tttcttatat atgcaatgcg ggttgaaaga ataaacataa ccgaaaggtt agaatactct
     5101 gagagttcaa aacccttttc tattaaaaat gaaattcttc caagatcacc cctgggatca
     5161 atcacggcca tagatgattt aagtgcaaaa ggcatagctt tttctgccac aattgtgccg
     5221 tcacaaccat aatcctcacc cacaattatc atggcccctc ctgtaacccc tgatgagcaa
     5281 atatgagcaa gagcgtccga ggctacattg gtaccgacaa tcttccagtt tactgagccc
     5341 ctaatagggt agtttattga agctgagaga agagctgcag cagatgcctc attgcccgaa
     5401 cagtcaaaat acataccata gtctttgagt accggatcat atgcatcaga tattacgtct
     5461 ataagacttg cagtaggagc tcccgggtat ccacctacat aacttacacc tgactgaaga
     5521 aatgatttta agatagtaag aggggtatca ccatataagg tctgaccctc tcccagattt
     5581 aactgcgata ttaaatcgtt agaataaact gatcccatgc catatatttt cactaataat
     5641 taattcgata tcaagaataa ttaaatatta aggttagatt aaaaattaac tgattggata
     5701 aatttcaatt ttatattgca attcggagct cgcacccgtt cctctgaagt tgccagaaat
     5761 gggatatgta ttttgtggca gtgaagaaga tgagagtgat atgtttttat ctcctacagc
     5821 aagtccaagg gttggatcat aacctttcca tccggctccc gggataaaaa cctctgccca
     5881 ggcgtgcagc tgcctggttt cagtatcaat atctatctca ttataaccgc ttgtaaatct
     5941 gcttggaaga ccaacacttc tgcagcactc tataaacaac actgcgaggt cacggcaaga
     6001 gcctttattt tcctttagag tcttaacagg tttccagggg ttgccggtta ctctaataat
     6061 tttttcaaaa tcctgataga tatagttatt aagattgaat aaaaattcta ctgtttgatt
     6121 gttggagtta ttgataattg gctcgatcaa atcagatatt tctttg
//
LOCUS       DBNGSV010000170         7018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1829634, whole genome shotgun sequence.
ACCESSION   DBNGSV010000170 DBNGSV010000000
VERSION     DBNGSV010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1829634"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="AC159U_06065"
     CDS             <1..553
                     /locus_tag="AC159U_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3336658.1"
                     /translation="AHSDVFNVLLQILDDGRLTDGQGRTVDFRNTVIIMTSNLGSHHI
                     QENIKDEKKLEENVMEVVKASFRPEFLNRIDEIVIYKPLSLDHIKEIVEIQVDHLRKR
                     LEDKKMDIELSDEAKEALAEQGYDPIYGARPLKRTIQKLIQNPLATELLKGEYKEGDL
                     IFVDVDKNKDFTFNKKENKKKAA"
     gene            638..868
                     /locus_tag="AC159U_06070"
     CDS             638..868
                     /locus_tag="AC159U_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HIR3336659.1"
                     /translation="MKVKTSITLSEDILRTMDKRLSDFKNRSDFIEQAIKSYIEKLKK
                     DEQDVKDLAILNKEADRLNKEAEDVLSYQTIP"
     gene            865..1191
                     /locus_tag="AC159U_06075"
     CDS             865..1191
                     /locus_tag="AC159U_06075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3336660.1"
                     /translation="MKRGELYLVQNPSSRDPKKQRVFIIVSRQVLIDSRFSTVICAPV
                     YSKYDGLSTQIPIGTKEGLKHESSIHCDELVSLPKTILTNYIGCLELKTIKSLDNALL
                     AALDIN"
     gene            complement(1184..1906)
                     /gene="pyrF"
                     /locus_tag="AC159U_06080"
     CDS             complement(1184..1906)
                     /gene="pyrF"
                     /locus_tag="AC159U_06080"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3336661.1"
                     /translation="MSSEQLNPYERIILALDFADFNESEKWVKRFSSKIKTYKVGPIL
                     YVKNGTDVLKRFSELGADIFLDLKFHDIPSTVEKTVRQLADLNINMFTLHALGGFDMM
                     KRVSESVAEEADKLSKQKPITLAVTVLTSHDESTLSEIGINNSTKDEVLKLAGVADKA
                     GIDGLVCSGQELEFIKQEFGDRFKLVIPGIRPASNVHDQKRVMTPEAAVSKGADYLVI
                     GRAITEAEDPDEVVEQIKKDIS"
     gene            complement(1955..3670)
                     /locus_tag="AC159U_06085"
     CDS             complement(1955..3670)
                     /locus_tag="AC159U_06085"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3336662.1"
                     /translation="MRYSQYFLPTLKETPADAEVVSHQLMVRAGMLRKVAAGIYDYLP
                     LGLRSIRKFEEIVREEMNRAGAIELLMPAVLPAEHWKESGRWDVYGKELLRFTDRAER
                     EFCIGPTHEEIITDVVRREVKSYKQLPVNLYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFDATDEGADKSYWAMYEAYSRIFERCGLEFRAVLADTGNIGGSFSHEFMVLAETGE
                     DVVMSCDKCDYAANLELAEIKQTDENNGTESEEQKDIEEVHTPDLKTVEEVAEFLKVQ
                     TGDLIKTMIIEADEQPLVALVSGDNELSITKLRRHLGADQVELATPDVIEKVTGGALG
                     FSGPVGLKVNIVADHSIKQIQNGVTGANKKDYHLVNVNLDKDYKVSEFADIRVAADGD
                     GCPNCDGGKLKSTRGIEVGHVFKLGTKYSEAMSANFVAEDGKEKPFIMGCYGIGIGRT
                     VAAAIEQNFDDSGMILPRALAPFEIIVMPLNMKDEDVVKLSDDIYNSLVEKGYDVLID
                     DRKESAGYKFKDADLIGIPVHVRIGPKTLKDNSVEIRLRSTGENKLVKIEDAVSEVDK
                     IMREI"
     gene            3847..4086
                     /locus_tag="AC159U_06090"
     CDS             3847..4086
                     /locus_tag="AC159U_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3336663.1"
                     /translation="MKASIVDLRYKMKEVIKALDRKETVTILYHGKKKGLIIPIHSKD
                     KKDITKHPFFGMNKADKKSIDKTMKELRESRIDVV"
     gene            4076..4453
                     /locus_tag="AC159U_06095"
     CDS             4076..4453
                     /locus_tag="AC159U_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3336664.1"
                     /translation="MLFDTDILIWVQRGNNKAAKLINDSVERFLSIQSYMELIQCAKN
                     KAQINYSKDFLYTYDFQVLPLTENIGHRASIYTEEYTLSSGLRAADAIIAATATENNM
                     VLATSNAKHFKPIKDLKLKIFKP"
     gene            complement(4463..4924)
                     /locus_tag="AC159U_06100"
     CDS             complement(4463..4924)
                     /locus_tag="AC159U_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family protein"
                     /protein_id="HIR3336665.1"
                     /translation="MEPRTSQPKLRLEAISELADSGIPVGVLIAPVIPGLTDHEMPKI
                     IEKSVDAGASYAAYVMLRLPFGVKDIFSTWLEQYYPDRKNKVLNKVNSVRDGKLYSSE
                     FFKRQRGEGVFADQAAKIFEIACRKNGIDNNSINLSTDHFINTGNKQMELF"
     gene            complement(4958..5518)
                     /locus_tag="AC159U_06105"
     CDS             complement(4958..5518)
                     /locus_tag="AC159U_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family protein"
                     /protein_id="HIR3336666.1"
                     /translation="MSSYKQKIKGRGTSDNPVNRFDKIDFVPDEEFYKDEQKPKTEFF
                     RDTSKSIITYNSSPDINFDASINPYRGCEHGCAYCYARPNHEHLGLSAGLDFETKIFV
                     KENAPELLKKELSSKMWKPQTIAISGVTDCYQPCEKHFELTRSCLEVLNEFKNPVGIV
                     TKNKMVTRDKDILKEMAKINAAAVTI"
     gene            complement(5889..6953)
                     /gene="ispG"
                     /locus_tag="AC159U_06110"
                     /gene_synonym="gcpE"
     CDS             complement(5889..6953)
                     /gene="ispG"
                     /locus_tag="AC159U_06110"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3336667.1"
                     /translation="MRRVTRQFSLGNVKIGGGAPVSVQSMTKTDTRDIDATVEQISRL
                     ENAGCDIVRLAVPDMEAARALGEIKNQTNIPLVSDIHFDYKLALEAIAQGVDGMRINP
                     GNIGSKKRIKAVVDAVKERKIPIRIGVNSGSLEKDILKKHGSPTPEALAESAMRHIRI
                     LEDMDFYDTKISVKSTDVMRMIEAYKLIAEKTDYPLHLGVTEAGTIKMGTIKSSVGIG
                     SLLAQGIGDTIRVSLTGDPVDEIVVGIDILKSLGHRKHGIELISCPGCGRLEIDLMKL
                     VADIEKKIEHIELPRPIKVAVLGCVVNGPGEASDADIGIAGGRGKGMMYKDGKLIRSF
                     KEDQIVDELAKELETFRVVQ"
BASE COUNT         2081 a         1493 c         1215 g         2229 t
ORIGIN      
        1 agctcatagc gatgtattta acgttctact gcagattctc gatgacggaa ggcttacaga
       61 cggacagggt aggactgtag actttagaaa cactgtgata atcatgactt ctaatcttgg
      121 aagccatcat attcaggaaa acattaaaga cgaaaagaaa cttgaagaaa acgttatgga
      181 agtggtaaag gcatcattca gacctgaatt tttaaacagg atcgatgaga ttgtgatcta
      241 caaaccttta tcattggatc atataaaaga gattgtagaa attcaggtgg accatttaag
      301 aaagagactg gaagacaaaa aaatggatat cgaactttcc gacgaagcaa aagaggctct
      361 tgccgagcag ggttatgacc cgatatatgg cgcgcgacct cttaaaagga cgatacaaaa
      421 gctcattcaa aatcctcttg caacagagct acttaaaggt gagtacaaag aaggtgattt
      481 gatatttgta gatgtagaca agaataagga tttcactttt aataagaaag agaacaagaa
      541 aaaagctgca taagattatt aagtgggaga aatttgagaa ataatttctt ccactgttga
      601 gaattgaaat ggaatattta tggggtataa tggtagtatg aaagtcaaga catcaataac
      661 actatcagaa gatattctaa gaacaatgga taaaagattg tctgatttta aaaacaggtc
      721 tgattttatt gaacaggcta tcaaatctta tattgagaaa ttaaaaaaag acgaacagga
      781 tgtaaaagat cttgctattt taaacaaaga agctgatcga ttaaataaag aagctgaaga
      841 tgtgctttca tatcagacaa taccatgaaa cggggtgaat tgtatttggt acaaaatcca
      901 tcttccagag accctaaaaa acaaagggtc tttatcatag taagccgtca agttcttatc
      961 gattcacgtt tttctaccgt tatatgtgct cctgtgtatt caaaatatga tggactatcc
     1021 acgcaaattc caataggaac aaaggaaggg cttaaacatg aaagcagcat tcactgcgat
     1081 gaacttgtga gcttgcctaa aacaattctt acaaactata ttggctgttt agaactcaaa
     1141 acaatcaaat ctctggacaa tgcacttctt gcagctttgg atatcaacta atatctttct
     1201 taatttgctc cacaacttca tcaggatctt ctgcttccgt gatagctcta ccaatcacaa
     1261 gataatctgc gccttttgat acggctgctt ctggagtcat tactcgtttt tgatcatgta
     1321 cattactagc tggtcttata ccagggatta caagtttaaa cctatcacca aactcctgtt
     1381 taataaattc aagctcctgc cctgaacata caagcccgtc tataccggct ttatcggcga
     1441 cacctgcaag cttcaacact tcatcttttg tagagttatt tatccctatc tcgcttaatg
     1501 tactctcgtc atgacttgta agcacagtaa ctgccagagt aattggtttt tgctttgaga
     1561 gtttatctgc ttcttctgca accgattcgg atactctttt catcatatca aaaccgccaa
     1621 gagcatggag ggtaaacata ttgatattca gatcagcaag ctgccttacg gttttttcta
     1681 cagtcgatgg aatgtcgtgg aatttgagat ctagaaaaat atcagcacca agctcggaaa
     1741 acctttttag tacgtcggtt ccgttcttaa cgtatagtat cgggccgact ttataggttt
     1801 ttatcttact actaaaccgt ttgacccact tctctgactc attaaaatca gcgaagtcca
     1861 gggctaatat tattctctca tagggattta gttgttcaga agacatgccc tattttaagc
     1921 tatttggaat gaatgtgaag taggaaaata aatattaaat ttctctcatt atcttatcta
     1981 cttctgatac tgcatcttca attttaacaa gcttgttctc gccggtgctc ctaagcctaa
     2041 tttcaactga attatcttta agtgtttttg ggccgatgcg aacatgtaca ggtattccaa
     2101 taagatcggc atccttaaat ttataacctg cgctctcttt tctgtcatca attaaaacgt
     2161 catagccttt ctctaccaaa ctgttgtata tatcatcgga tagcttaacc acatcttcat
     2221 ctttcatgtt tagtggcatc actattatct caaatggtgc aagcgccctt ggaagtatca
     2281 taccgctgtc atcaaaattc tgttcaatgg ctgctgcaac tgtcctgcca attccgatcc
     2341 cataacatcc catgataaat ggtttttcct ttccgtcctc tgctacaaaa ttggcactca
     2401 tggcctcgct gtattttgtg ccgagtttga aaacgtggcc aacttctatt cctcttgtgg
     2461 atttaagttt tccgccatca cagtttggac acccgtctcc gtctgctgcc actcttatgt
     2521 ctgcaaactc gcttacttta taatccttat ccaggttcac gtttacaaga tgatagtcct
     2581 ttttatttgc tccggtaact ccgttttgaa tttgttttat tgaatggtct gcaacgatgt
     2641 taacttttag accaactggc ccgctaaacc caagagctcc accggttacc ttttcaatta
     2701 catcaggagt tgcaagctct acctgatctg cacccagatg tcttctaagt ttagtaatgc
     2761 taagctcatt atccccggag acaagcgcta caagcggctg ctcgtctgcc tcgatgatca
     2821 tggtcttaat aagatcacca gtctgtactt ttaaaaactc ggcaacctct tctactgtct
     2881 ttaaatccgg tgtgtgtact tcttctatat ctttttgttc ttctgactca gtaccattgt
     2941 tttcatctgt ctgcttaatt tcagcaagct ccaggttggc agcgtaatcg catttatcac
     3001 agctcatcac cacatcctca ccggtttctg caagcaccat aaattcatgt gaaaaactac
     3061 cgcctatgtt tccagtgtct gcaagtactg ccctgaactc gagtccgcat ctctcgaata
     3121 ttctgctata cgcctcgtac atcgcccagt atgatttatc tgcgccttca tccgtggcat
     3181 caaagctgta tgcgtctttc atgatgaact ctcttgcgcg catgactcca aaacgtggac
     3241 gtatctcgtc acgaaacttg gtctgaatct gatacaggtt tacaggcaac tgcttgtaag
     3301 actttacttc tcttctaaca acatcggtaa taatctcttc atgagtaggc ccaatgcaaa
     3361 actccctctc tgcacggtct gtaaacctta gcaattcttt accatagaca tcccatctgc
     3421 cgctctcttt ccagtgttct gcgggaagta ccgccggcat tagaagttca atagccccag
     3481 ccctgttcat ctcctcacgc actatctctt caaattttct tatcgagcgt agcccaagtg
     3541 ggaggtagtc atatataccc gctgcaactt tcctgagcat ccctgccctt accataagtt
     3601 ggtgacttac aacctctgca tcagcaggtg tttctttaag agttggtaaa aaatattgtg
     3661 aatatctcat gacttaaggg gtataactta ctgattttga gtttaaatgc aatataaatc
     3721 ttacaatata caatacatca ctcaatttag attgaaactg tttatccaca gattgaacag
     3781 aaatatggct tgaaagcagt gattgataaa tgtataatac caacatacat tattgaggga
     3841 tatattatga aagcatcaat agttgatctt agatacaaga tgaaagaagt aatcaaagca
     3901 ctggacagaa aagaaactgt tacaatctta tatcatggta aaaaaaaggg gttgattata
     3961 cccatccaca gtaaagacaa aaaagatatt accaaacacc cattttttgg catgaataaa
     4021 gcagataaga aatctattga taagactatg aaagaactaa gggaatctag aatagatgtt
     4081 gtttgatact gatatattaa tttgggtcca aaggggaaac aataaagctg caaaactaat
     4141 taatgattcc gttgaaagat tcctgtccat ccaatcctat atggaactta ttcagtgtgc
     4201 taaaaacaaa gcacaaataa attactctaa agatttcctt tatacatatg atttccaggt
     4261 acttcccctc acagaaaata ttggtcatcg agcatccata tatactgagg aatatacact
     4321 atcttcaggt ttgcgtgcag cagatgcaat aatagctgcg acagctacag agaataatat
     4381 ggttcttgct acaagcaacg caaaacattt caaacctata aaagacttaa aattaaagat
     4441 atttaaacct taataaaaat tatcaaaaca attccatttg tttgtttcct gtattaataa
     4501 aatgatcagt agataagttt atagagttat tatctattcc gtttttccta caagctatct
     4561 caaatatttt agccgcttga tctgcaaaaa ctccctctcc tctctgcctt ttgaaaaact
     4621 ccgaagaata tagtttacca tctctaactg aattaacctt gttgagtact ttgttctttc
     4681 tgtctggata gtactgctca agccaggtag aaaaaatgtc tttgactcca aacggaagcc
     4741 tgagcattac ataggctgca taggatgcac ctgcatctac agatttctct ataatctttg
     4801 gcatctcatg atctgttagt cccggaataa ccggtgcgat caaaacccct acaggaattc
     4861 cgctatctgc aagctcgctg attgcttcta atctcagttt aggctgggag gttcgtggct
     4921 ccattaacct tgcaagctca ggctcaaggg tcgttattta aatagtcaca gcagcagcat
     4981 taatttttgc catttcttta agaatgtcct tatctcttgt aaccatttta ttcttggtaa
     5041 caatcccaac aggattctta aattcgttga ggacctctaa acatgaacga gtaagttcga
     5101 agtgcttctc gcatggctga taacaatcgg ttaccccgct tattgctata gtttgaggct
     5161 tccacatttt tgatgacagc tcttttttaa gaagctcagg tgcattttct tttacaaata
     5221 ttttggtttc aaaatcaagc cctgcagata gtcccagatg ctcatggttt ggccttgcat
     5281 agcaatacgc acacccgtgc tcgcagcccc tgtatggatt gatgctcgca tcaaaattga
     5341 tatctgggct ggaattataa gtaattatgg actttgatgt atccctaaag aattcagtct
     5401 ttggtttctg ctcatcttta taaaactctt catccggaac aaaatcaatt ttatcaaatc
     5461 tgttaacagg attatcagat gttccccgac cctttatttt ttgtttatat gaactcataa
     5521 atatatgcac taaattcttc agcccagtta gctgaatgtc aatatcaaaa taataaagag
     5581 ggatttttga tctatatcgg caaggagtca gatgcccctg cgatccaaga attaaaaaag
     5641 tttaatctta attgacattt tgttataata tatatgtgga aagaaagtat attttcttta
     5701 aaacaagcag gggattttgg gcaccattct ttctttttgg ttgtttctaa ttcctttcat
     5761 aattttagtc tggatttttg atttgtcagt agtaggaaga ataatgttat cactcatatt
     5821 cgcactactt ataagtcatc ttgcttttga aatagacaag aagaaaagtt cggagaaata
     5881 aatctaaatc actgcacaac cctaaaggtc tctaactctt ttgccagttc atctacaatc
     5941 tgatcttctt taaaagagcg gatgagtttt ccgtctttat acatcatgcc cttccctctt
     6001 ccgcctgcaa ttcctatatc ggcatcagag gcttcgcctg ggccgtttac tacacatcct
     6061 agaaccgcaa cttttattgg tcttggaagt tcgatatgtt cgatcttttt ctcaatatca
     6121 gcgacaagtt tcatgagatc aatttccagc cttccgcacc ctgggcacga aataagctca
     6181 atcccatgct ttctatgacc aagagatttt aagatatcta tacctacaac tatctcatcc
     6241 acaggatcac ccgtaagtga tactcttatg gtatcaccaa taccctgagc aagcaggctg
     6301 cctatgccga ccgaagattt tatagtaccc attttaattg ttcccgcttc agtaacacca
     6361 agatgaagcg gataatcggt tttttctgca atcaacttat aagcctctat catcctcata
     6421 acatcggttg atttaacaga aattttggta tcataaaagt ccatatcctc taaaatccgg
     6481 atgtgcctca ttgcactctc tgctaaagcc tcgggagtgg gacttccgtg ttttttaagt
     6541 atatcttttt cgagcgagcc ggaatttacg ccaattctaa ttggaatttt tctctctttt
     6601 acagcatcta ctacggcttt tattctcttt tttgatccta tattacctgg atttattctc
     6661 attccgtcta caccctgagc aattgcctca agtgccagct tgtaatcaaa atgaatgtct
     6721 gatacaagcg ggatattggt ctgattcttt atctcgccaa gggcccttgc tgcttccata
     6781 tcgggtacag caagcctcac aatatcacac ccggcgtttt ccagccggct tatctgctcc
     6841 acagtggcgt caatgtccct agtatcggtc tttgtcatag actgcaccga aaccggagct
     6901 ccaccaccga tttttacatt tcctaaactg aactgcctgg ttactcttct catttttcgc
     6961 tactaggtta gtttaaataa catgtagtgt caagatggca aatagtacta tatattgt
//
LOCUS       DBNGSV010000171         8783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_722216, whole genome shotgun sequence.
ACCESSION   DBNGSV010000171 DBNGSV010000000
VERSION     DBNGSV010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_722216"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="AC159U_06115"
     CDS             <1..747
                     /locus_tag="AC159U_06115"
                     /inference="COORDINATES: protein motif:HMM:NF036799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR3336668.1"
                     /translation="IQSVLDNYKRNLNNQAVDIKLFEISRTYEKTKNGMEENLKLCIA
                     ASVENDNELWDKRKYDFFDLKNLLINLISFLRLDGRVALEADASSISFVHPGKSALVM
                     SDGFEIGYLGYLHPDFLSSLDIDTDVIISEIDLSALLETQKQNVSKFTPIPRFPYTKR
                     DLSLVVDSSISVKRILEEIKKTENELIENVKIFDVYSEGDLAKTGQKSVSISLIIRSK
                     ETTLTDEQANLVQSSIFDKLSESLDARMRS"
     gene            766..1047
                     /locus_tag="AC159U_06120"
     CDS             766..1047
                     /locus_tag="AC159U_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3336669.1"
                     /translation="MTKKELGEVLHSRIGLSKRESSEIVEFFFNNLKEILSNGQSIKL
                     PRFGSFRTQNRNARKGRNPSTGEDITIGERKVVVFKSSRFLRDALNSEQ"
     gene            1054..1719
                     /locus_tag="AC159U_06125"
     CDS             1054..1719
                     /locus_tag="AC159U_06125"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR3336670.1"
                     /translation="MSHISGSIKRSFLTGLLVIIPFGLTLFVVYKISLWVVRFISFVP
                     AKFIEPLSVLPKPLYQYATFSIGLIGALIIIIAVGIIARNYLGGKLLNFSENIISKIP
                     FARSIYSASKQMIETVLFSRGFKGIKRVIMLEFPRKGIFCLGFVTGNVANKAIDDTDD
                     TLLSIFVPNTPNPTSGYYLMLPESEVQEVSITVEEAFKVIISGGLAAKDFELIKKSTP
                     VDY"
     gene            1682..3046
                     /gene="tilS"
                     /locus_tag="AC159U_06130"
     CDS             1682..3046
                     /gene="tilS"
                     /locus_tag="AC159U_06130"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3336671.1"
                     /translation="MSLLKKVHQSITKHNLLSKNDTIIVGVSGGADSVFLLHALSQLN
                     QYELSIIVAHVNHCLRGKESDRDAEFVKKVCKDRGLEFELLVENASVIKKELGKGIEE
                     SARIIRYKFFEFLTKKYGADKVATAHTADDQLETVFMRLIRGSGLKGLSGIPIKRDDI
                     VRPLLEITKDEIKKYLKENKIKWVEDSSNAEIDFFRNRVRHKLVPVLKELNPNITENV
                     LRTSYQFTEKDSFVSEHTSKLFKKLFRKTAEAFYTTSLKKFKKTHRLIQFTLLREAVS
                     RVNENLLNIDFDHIEAALELMSSKKISGEVNLPNNLIIAKSYDDLIVTKKSVINPNYS
                     YKIYDFGQYDFEHVSFVIKKTKTKNLDQGTDVALLNPKKVKFPVEIRNWRPGDKFTPF
                     GMKGEKKLKDFFIDEKIPRYLRKLYPLFIINREIAWVGGLRMSDKFRVKGDIAIKLKI
                     IKTK"
     gene            3070..3984
                     /locus_tag="AC159U_06135"
     CDS             3070..3984
                     /locus_tag="AC159U_06135"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HIR3336672.1"
                     /translation="MDYQLILEEIYEEIKPYLALGKVADYIPELKKVSPDNFAMVLQT
                     ADGEISKVGDFNTKFSIQSISKLFTLTLALQFEDKRLWQRVGREPSGNPFNSLVQLEY
                     EKGIPRNPFINAGALVITDIILSRVGDAKKAIIDYLRLLSGNSSIKHDPKVYASEKKT
                     GYLNKSIANYLKSFNNLDNQPSGVLDVYFYQCSISMNCIDLSNACSYLVHSGYSPLIK
                     EAVSTTRQTKYLNSLLLTCGTYDAVGDFAYRVGLPGKSGVGGGIIAVLPGQFNIAVWS
                     PGLNDNGNSLAGVKALEMFTTITGKSIF"
     gene            complement(4042..4998)
                     /locus_tag="AC159U_06140"
     CDS             complement(4042..4998)
                     /locus_tag="AC159U_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3336673.1"
                     /translation="MSRLVIGIDIGGTNLRGAVVDSKGNIQKRYSISSEANIGIDNLI
                     DNLIHLISNLRSDYKIAGIGIGIPGILDSKNGILTQAPNINVANNYPIRKVLSKRIKP
                     KVPIFIENDANCAAFGEFKFGAGKGLNSLIMLTLGTGVGGGIILNKEIWNGAYGMGGE
                     IGHIKIYPGGIKCNCGHRGCLESYSSQAGIYNMVKKGRKENRINKTLSDLIKSTHKDK
                     LPELIFRQAKNGSKFSVKLWEEFGTALGIGISSITNLLNPEIVVIGGGITGAWQFFIP
                     SLKKTVKENTLSGPYKKLIISRSKLKDDAGILGAANLALGKK"
     gene            5099..5674
                     /locus_tag="AC159U_06145"
     CDS             5099..5674
                     /locus_tag="AC159U_06145"
                     /inference="COORDINATES: protein motif:HMM:NF047071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3336674.1"
                     /translation="MTNKEKILHAAYTMIYKNGYGQTSVDEIIRKAGVSKSNFYYHFR
                     SKQLLGLEVLEMRIDNYISEVLDKTLSNENLSPLRRLNSFYDKVISIHENDKCRHGCP
                     FGNLAIELSSKNSSFRKRLEQFFELWKQKIQNCLQEGVRIGEFSDKFDTAISSELILS
                     HLQGAILMTKTYKQINPLDNGSKEIINLLKA"
     gene            5702..6409
                     /locus_tag="AC159U_06150"
     CDS             5702..6409
                     /locus_tag="AC159U_06150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3336675.1"
                     /translation="MSDYMLKSNISADIVHLKEINPDLYGSWMDYHNTAFTDGALTKK
                     EKELIAVAAAHLTRCSYCIRSRVTISKKHGTSDEEITEAIYVAMRFAMGAPYAYTSMA
                     YEAWDALDNDVPLTEGHFFKKNIAHEIKCFSDASGESDDKFKDFTKKVFADGALSKNF
                     KRGLLGLCCANMTRCPYCIRGCVKDARAAGYTKEQIAEAMNVGMVMSAGACYAHSSVA
                     MYTLDSINDKEQKKTGS"
     gene            6489..7208
                     /locus_tag="AC159U_06155"
     CDS             6489..7208
                     /locus_tag="AC159U_06155"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3336676.1"
                     /translation="MIVRINRYISMCGYTSRRKADELIDKGEVYVNDKLVTEPGLSVD
                     TLSDTVRIGEQELNFENKRYILLNKPKLYVTTLENNEDDKKTIVELIDDIPERVYPVG
                     RLDYDVEGLLFLTNDGEIAHKIHHPSFTVPKEYVCVLNKKITLQELEKLTEGAELEDG
                     FVKPDSLKIPVNNNNSNLVVATFHEGKNHLVKRYFSHFGYKIIKLKRVSIGPLTLGDL
                     QKGKWKDLSKKELEGLFDYFN"
     gene            complement(7228..7734)
                     /locus_tag="AC159U_06160"
     CDS             complement(7228..7734)
                     /locus_tag="AC159U_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336677.1"
                     /translation="MQPRKHIAIIIIFILIFGLQTKLGFSRNYIESLKVKAIPAEILD
                     TICPLYLTFQMQIIAGVKMYKTEQLTIKYRLIGDNGFASNWRQYNIAKGDTRTDVFRR
                     RIDPHAIAEKTDIKNNPTLELKGDKIHYRGWSAVELIYQDELRKIYTVGSNKAEFNIE
                     CKELEIIN"
     gene            7837..8751
                     /locus_tag="AC159U_06165"
     CDS             7837..8751
                     /locus_tag="AC159U_06165"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR3336678.1"
                     /translation="MIKRYSWQFTEGKIRRIFSHEKYGKNKTCKRCKSRVLHRLRDQR
                     HECSKCQFRFSLKTDTYLKHSRVSLDVWYELLWWFVYGFTANKTAKETQLSQKLAHRC
                     FTIIRKALFDYEERNIKPVFGTVEVDETYVGPKFKNRRKKDREYYRRVEAVKRGRGAK
                     TLLQPVFGFYQRNGTVYVEFVRDAEKKTLQDIIKGRIVLRSKIYTDTWRSYRGLNRKG
                     YYHKTIDHGKQEYVKQGKDNKVHINGIEGFWGYLKEQLLKHHGVSKANLIYYVKEQEF
                     RFNNRHLSTDDMVKKIIKILMNSTPSDV"
BASE COUNT         2986 a         1419 c         1733 g         2645 t
ORIGIN      
        1 attcagagcg tgcttgataa ttataagaga aatctcaaca atcaggcggt agatattaag
       61 ctctttgaga tttcaaggac ttatgaaaag actaaaaatg ggatggagga aaatctaaag
      121 ctttgtatag ctgcatcagt tgaaaatgat aatgagctat gggataaaag aaaatatgac
      181 ttttttgatt taaagaactt gcttattaat ctgatttcgt ttttaaggct ggacggcaga
      241 gtagcccttg aagctgatgc ctcaagcatt agctttgttc atccgggaaa atcagctttg
      301 gttatgtcag acggatttga aattggttac ttgggatatc tgcatcctga ttttttatcg
      361 agtcttgata tagatactga tgtaattata tcagagattg accttagtgc tttacttgaa
      421 acacagaaac aaaatgtcag caaatttaca cctattccaa ggtttcccta tacaaaaagg
      481 gacttgtcat tagttgtaga tagtagtata agtgtaaaga ggatactgga agaaattaag
      541 aaaacagaaa atgaattaat agagaatgta aaaatatttg atgtttacag tgagggtgac
      601 ctggccaaga cgggtcaaaa gagtgtttcc atttcactta ttataaggtc caaggaaacc
      661 acacttactg atgaacaggc aaatcttgta caatcttcta tatttgataa actaagtgaa
      721 agcttagatg cgcgtatgcg ttcctgataa taaagaggtg tgggtatgac taaaaaagag
      781 ttgggagaag tattacattc aaggattgga ctatccaaaa gagaatcatc agaaatagta
      841 gaatttttct ttaataacct aaaagaaata ttaagtaacg gacagtcaat caagcttccg
      901 agatttggaa gttttagaac tcagaataga aatgccagaa aaggcaggaa tccttcaacc
      961 ggagaagaca taactattgg tgagagaaaa gtggttgtgt tcaaatccag caggtttttg
     1021 agggatgccc taaattccga gcaataatat ttaatgagtc atatatcggg ttctattaaa
     1081 agaagttttc tcacgggatt gctggtgata atcccattcg gactgactct ttttgttgtt
     1141 tataaaatta gtctctgggt agtaagattt attagctttg tacccgcaaa gtttatagaa
     1201 cccttatcag tgcttccaaa acccctttat caatacgcca ctttttcaat tggcctgatc
     1261 ggtgcgctta taataattat tgctgttgga attattgcca gaaactacct tggtgggaag
     1321 ctccttaatt ttagcgaaaa cattatctca aaaattccat ttgcaaggtc aatttattct
     1381 gcctcaaagc aaatgattga gactgtgctt ttcagcagag gatttaaggg tattaaaagg
     1441 gtaatcatgc ttgagtttcc aagaaaaggt attttctgtc ttggatttgt tactgggaat
     1501 gttgcaaaca aggcaataga tgataccgac gatacactgc ttagtatttt tgtaccaaat
     1561 actccaaacc caacttcagg ctattatcta atgcttccgg aaagcgaagt ccaggaagta
     1621 tcgatcacag tcgaggaagc atttaaagta ataatatccg gggggcttgc agcaaaagat
     1681 tttgagctta ttaaaaaaag tacaccagtc gattactaag cataatctgc tttccaaaaa
     1741 tgacaccatt attgtggggg tatccggcgg ggcagactct gtctttttat tacatgcatt
     1801 atcacagtta aatcaatatg agcttagtat tatagtagct catgtaaacc actgtcttag
     1861 gggaaaagaa tctgacagag atgctgaatt tgtaaaaaag gtgtgtaaag acagggggtt
     1921 ggagtttgag cttctagttg aaaatgcttc agttataaaa aaggagcttg gaaagggaat
     1981 agaggagtcg gcccgtatca taagatataa gttctttgag tttttaacca agaaatatgg
     2041 cgcagacaaa gtagcaacgg ctcatacggc agatgatcag ttggagaccg tgtttatgag
     2101 gcttatacgc gggtcaggac ttaaaggtct ctctggaata cccattaaaa gagacgatat
     2161 tgtaagaccg cttttggaaa tcactaaaga tgaaataaaa aaatatctaa aagaaaataa
     2221 aatcaaatgg gttgaagata gttccaatgc ggaaattgat ttttttagga atagagtaag
     2281 acacaagctt gtaccggtct taaaagagtt aaaccctaat atcaccgaaa atgttcttag
     2341 aacctcttat cagtttacag aaaaagactc ttttgtttca gagcatacga gtaagctatt
     2401 taaaaaatta ttcaggaaaa ctgcagaggc tttttatact acgagtctaa agaaatttaa
     2461 aaaaacacac agattgattc agtttacttt actaagagaa gcggtttcca gggttaacga
     2521 aaatcttcta aatattgatt ttgaccatat agaggcagca cttgaactga tgtcttcaaa
     2581 aaagatttca ggcgaagtaa atcttccaaa taatttaatc atagcaaaga gttatgatga
     2641 tttaattgtc acaaaaaagt cagtgattaa tcccaattat tcatataaaa tttatgattt
     2701 tggacaatat gactttgaac atgtttcatt tgttataaag aaaactaaaa ctaaaaatct
     2761 tgatcaaggc acagatgttg cactgctaaa tcctaagaaa gtaaagttcc cggtagagat
     2821 tagaaactgg aggcctggtg ataaattcac tccatttgga atgaaaggag agaagaaatt
     2881 aaaggacttt tttattgatg aaaaaatccc cagatactta agaaagctct atcccctttt
     2941 tattataaac cgtgaaattg cctgggttgg tggcttaaga atgtcagata agtttagggt
     3001 taaaggtgat atagcaataa aattaaaaat aattaagact aagtaatcaa gcaatatgta
     3061 aacacggaaa tggattacca gctaatttta gaagaaatat atgaagagat aaagccttat
     3121 ctagccttgg gtaaggttgc cgattatatt cccgagctta aaaaggtctc acctgataat
     3181 tttgccatgg ttttgcaaac cgcagatgga gagatttcaa aagttggtga ttttaacaca
     3241 aagttttcaa tacagagcat atcaaaactg tttacattaa cactggcatt gcaatttgaa
     3301 gataaaagat tgtggcaaag agtgggacgt gaaccatcag gaaatccatt taattctctc
     3361 gtacagctcg aatatgaaaa aggtatacca agaaatcctt ttattaatgc aggtgcatta
     3421 gtaataaccg acattatctt atcaagggtg ggagacgcaa aaaaagccat aatcgattat
     3481 cttaggttat taagtggcaa ttcgagtatt aaacatgatc caaaagttta cgcatcggaa
     3541 aagaaaacgg ggtatctgaa taaatctatt gccaactatc ttaaaagttt taataatctc
     3601 gataatcaac cctctggtgt tttggacgtt tacttttacc agtgttcaat atctatgaac
     3661 tgtatcgatt tatccaatgc atgctcatat cttgtgcaca gcggctattc acctttaatc
     3721 aaagaggcag tctctacaac aagacaaacc aagtatctaa actcactgct ccttacatgt
     3781 ggaacctatg acgcggttgg tgattttgcc taccgggtag ggcttccggg caaaagtggt
     3841 gtgggtggag gcataatagc tgtgcttccc ggtcagttca atatcgccgt gtggtcgccg
     3901 ggtttgaatg acaacggaaa ttcccttgca ggggtaaaag ctctggagat gtttacgact
     3961 atcaccggca aatctatttt ttaacccgct tccatgatca aattaatctt ttgttatact
     4021 taaatgactt attcggcact tttatttttt tcccagtgcc agatttgctg cacctaaaat
     4081 tcctgcatcg tcttttaatt tagatctgct gattatgagt ttcttatagg gacctgaaag
     4141 tgtattttct tttacggtct tttttaaaga tggaatgaaa aattgccagg cgcccgtaat
     4201 cccgccgcct attacaacta tttcagggtt tagcagattt gtgattgaag aaattccaat
     4261 cccaagagcc gttccaaatt cttcccacag ttttacagag aatttgctgc catttttagc
     4321 ttgcctgaat atgagctcag gtaatttgtc cttatgtgtg gatttgatta aatccgatag
     4381 agtcttatta attctatttt cctttctacc cttttttacc atgttataaa tgcctgcctg
     4441 tgatgaataa ctttccagac aacccctgtg accacagtta cattttattc caccggggta
     4501 aatctttatg tgtccgatct ctcctcccat tccatatgcc ccattccaga tctctttatt
     4561 taaaatgatt cccccgccaa ctccggtacc aagagtaagc ataattaaac tatttaaacc
     4621 cttacccgcc ccaaatttaa actccccaaa tgctgcgcaa ttggcatcat tttctataaa
     4681 aatcggaact ttgggtttaa tccttttgct gagcactttt ctgatcggat aattattggc
     4741 cacattaata ttaggtgctt gcgtgagtat tccattttta gagtccagta ttccgggtat
     4801 tccaatacca atgcctgcaa ttttatagtc tgaccttagg ttacttatta aatgaataag
     4861 attatctatg aggttgtcta taccgatatt ggcctccgaa cttatgctgt atctcttttg
     4921 gatgtttcct ttagagtcaa ccactgcacc ccttagattt gtaccgccaa tatctatgcc
     4981 tattacgagt ctgctcattt aaagaaaatt acactaaatc tacaaataaa taaaacctct
     5041 ttacattttc aatttatatt atatattagt acgtactagt tagtcctaat ttaataaaat
     5101 gacaaacaaa gaaaaaatat tacacgctgc ttatacgatg atttataaaa atggttacgg
     5161 gcagacaagc gtagacgaga taataagaaa agcgggtgta tccaaaagca atttttatta
     5221 tcatttcaga agcaagcagt tgctgggtct tgaagtttta gaaatgagaa tagacaacta
     5281 tatatcagaa gtgctcgaca aaaccttatc gaatgaaaac ttaagtcctc tcagaagact
     5341 gaatagtttc tatgataaag tcatatccat acacgagaat gataaatgca gacacggatg
     5401 cccttttgga aatcttgcaa ttgagcttag ctctaaaaat agtagtttca gaaaaagact
     5461 ggagcagttt tttgaactct ggaaacagaa gattcaaaac tgtctgcagg aaggtgttag
     5521 gattggagaa ttctcagaca aatttgatac agcaatatcc tcagaactga tcctttcgca
     5581 tttacaaggt gcgattctca tgacaaaaac atacaaacaa attaaccctt tggataacgg
     5641 ttcaaaagaa ataataaatt tattaaaagc ctaatagcag ggctttgagg agaataaaaa
     5701 tatgagtgat tacatgctta agagtaatat cagtgccgat atagtacacc taaaggaaat
     5761 aaaccccgat ctttatggtt catggatgga ttatcacaat actgcgttta cagatggtgc
     5821 attaactaaa aaagaaaagg agctaattgc cgtagctgct gcacatctta ccagatgttc
     5881 ctattgcata agatcaagag taactataag taagaaacac ggcacatctg atgaggaaat
     5941 tacagaagca atatatgttg cgatgaggtt tgcaatgggc gctccttatg cttatacaag
     6001 tatggcctat gaagcttggg atgcactgga taatgatgtg ccgcttaccg aaggacattt
     6061 ttttaagaaa aatattgcac acgaaataaa atgtttttcc gatgcaagtg gggaatctga
     6121 cgataaattt aaagatttca ccaaaaaggt atttgctgat ggtgctttat caaaaaactt
     6181 caaacgtggc ctgcttggtc tttgctgtgc taatatgaca aggtgtcctt attgtataag
     6241 aggctgcgta aaagatgcaa gagcggccgg atatactaaa gagcagatag cagaagccat
     6301 gaatgttggt atggtcatgt cagcaggagc atgttatgct catagtagtg tagcgatgta
     6361 tacacttgat agtattaatg acaaagaaca gaagaaaacc ggttcgtagt ataattaaat
     6421 aaggatttat aaaaatataa gctccttttg ttatcaaagg agcttttttg ttttttatag
     6481 aataaaaaat gatagtaaga ataaaccgat atatatcgat gtgcggctat acgtcaagaa
     6541 gaaaggccga tgagctgatt gataaaggcg aggtatatgt aaacgacaaa ctagttactg
     6601 agcccggact ttccgtagac accttgagcg atactgtaag aattggtgag caggagctaa
     6661 attttgaaaa taagagatat atattactaa acaaaccaaa gctctatgta acaacacttg
     6721 aaaataacga agatgataaa aagacaatag tggaattgat tgatgatatt cctgaaagag
     6781 tctaccctgt ggggaggctg gactatgatg tagaagggct gcttttctta actaatgacg
     6841 gtgaaattgc tcataaaatt catcacccaa gctttacagt tccaaaggag tatgtctgtg
     6901 tgctcaataa aaagatcacc cttcaagaat tagaaaaatt aactgaaggt gccgagcttg
     6961 aagatggatt tgtaaaaccg gacagcttaa aaatccctgt aaacaataac aacagcaatc
     7021 ttgttgtagc cacatttcac gaagggaaaa atcatctcgt aaaaagatat ttttcccatt
     7081 tcggatataa gataataaag cttaaaaggg tatcaatagg gccgcttact cttggggatt
     7141 tacaaaaagg aaaatggaaa gatttgtcaa aaaaagaact tgagggtctc tttgattatt
     7201 ttaattaaat aagatttcta aaaagattta attaataatc tcaagttcct tgcattcaat
     7261 attgaattct gccttatttg atccaactgt atatatcttc cttaattcat cctgatatat
     7321 gagctcaact gctgaccatc ctctatagtg aatcttatcg ccctttaatt ctaatgtcgg
     7381 gttatttttg atatccgttt tttcagcaat tgcatgggga tcaatccttc ttctgaatac
     7441 atctgttctt gtatctcctt tggcaatatt gtattgccgc cagtttgatg caaatccgtt
     7501 atctcctatc aatctgtatt ttattgtgag ctgctcggtc ttgtacattt ttactccggc
     7561 aataatctgc atttgaaagg ttaagtacag tggacatatg gtatccagaa tttctgcagg
     7621 tatggctttt acttttaggc tttctatata gtttcttgaa aatccaagct tggtctgtaa
     7681 tccaaaaatc agaataaaaa tgataataat tgctatatgt tttcttggtt gcatagatat
     7741 tcctttgcga aattttatat tttataactg cataagtata atattttaac ttagggcatt
     7801 aaacgttgtt aggagtagta tatatgataa agagcaatga taaagcggta ttcatggcaa
     7861 tttacagagg gaaaaataag gaggattttt tcacatgaaa aatatggaaa aaacaaaacg
     7921 tgcaagcgat gtaaatcaag agtactgcac agactccgtg atcaaagaca tgagtgttca
     7981 aagtgtcagt ttcgtttctc acttaaaacc gatacatatc ttaagcacag cagagtgtca
     8041 ctggatgttt ggtatgaact gctatggtgg tttgtatacg gatttacagc aaataagacg
     8101 gcaaaagaaa cacagttatc acaaaagctg gcacacagat gttttacgat cataagaaaa
     8161 gctctatttg actatgaaga aagaaatata aaacctgtgt ttgggacagt ggaagtagat
     8221 gagacatatg tggggcctaa gtttaagaac aggaggaaga aggacaggga gtattacagg
     8281 agggtagaag cagtaaagcg tggaagaggt gctaaaacac tgcttcaacc ggtgtttggg
     8341 ttttatcaga gaaacgggac agtgtatgtg gagtttgtaa gagatgcgga gaaaaagact
     8401 cttcaggata taataaaggg aagaatagtt ttacgaagta aaatatacac cgatacctgg
     8461 agatcctaca ggggattaaa tagaaaaggt tattatcata aaaccataga tcatggcaaa
     8521 caggaatatg ttaaacaagg caaagacaat aaagtacata taaacggaat cgaggggttt
     8581 tggggatatt tgaaggagca gttattaaag catcatggag taagcaaggc aaatctgata
     8641 tattatgtga aggaacagga atttaggttt aataacagac atctgtctac ggatgatatg
     8701 gttaaaaaaa taatcaaaat cttaatgaat tctactccgt cagatgtcta atgccctaaa
     8761 tataatatcc tgtatagaga aag
//
LOCUS       DBNGSV010000172         1207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1692506, whole genome shotgun sequence.
ACCESSION   DBNGSV010000172 DBNGSV010000000
VERSION     DBNGSV010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1692506"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..355)
                     /locus_tag="AC159U_06170"
     CDS             complement(92..355)
                     /locus_tag="AC159U_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3336679.1"
                     /translation="MKETGLLQKILDKLQAAGKDSDGKSELSDNELIQRFVDNNDAAA
                     FEMIVSRYMEKIYSLAYRITRDHYKSEDVMQEVYMGIIAKNVC"
     gene            complement(500..>1207)
                     /locus_tag="AC159U_06175"
     CDS             complement(500..>1207)
                     /locus_tag="AC159U_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3336680.1"
                     /translation="DKDLDTLMDRLGSRVEAMKTVGNKQALILSIVLMAVALFISVYF
                     INMVNALLIVLAILSYTVLYTLYLKRSSPYGTILGGIPGALPVLIGYAAVEPSLGIGA
                     WILFIFMMLWQPPHFWALAQKYKDDYAKAKIPVMPVVFGTQYTNIFILLYSISLIPLS
                     LSLWFFGICTEYYAILVLIVGIYFQYVMLSSVISKSNYGKAFGVSILYMLCFMLILIL
                     DIYFNTDNSQNIFPLYR"
BASE COUNT          407 a          224 c          213 g          363 t
ORIGIN      
        1 atgggcttac agaaaccata gaggactggc taaacacaag agaattaatt acttcaaatg
       61 gtacaccttt ttaaagcaaa tctgactcaa attagcacac attttttgca attatgccca
      121 tatagacttc ctgcattaca tcctctgatt tgtaatgatc cctggttatt ctgtaagcaa
      181 ggctataaat tttttccata tacctgctta caatcatctc aaatgcggct gcatcgttat
      241 tatccacaaa cctttggatc agttcattat cagataattc actttttcca tctgagtctt
      301 tgccagcagc ttgtagctta tccagaattt tctgtagaag tcccgtttct ttcataaaaa
      361 tcaattgccg aatctattat aattttttat cgctcgaatt aagcgcagtt ttcctatatt
      421 aataaaaacc tgtattttta gatgaaaaaa atgggagttg gtttctaaat atttgaacaa
      481 atactaaaaa aagtaaaaac tacctgtaaa gtgggaaaat attctggctg ttatctgtat
      541 taaaatatat gtcgagtatt aaaatcagca taaaacacag catatataga attgaaacac
      601 caaatgcttt gccgtaattg ctttttgaaa ttacgcttga aagcatcaca tactggaaat
      661 aaatgcctac aatcagcact aaaatcgcat aatattcagt acatattcca aaaaaccaga
      721 gtgagagtga caggggaatt agagatatag agtatagaag tatgaatata ttggtatatt
      781 gtgtcccgaa tactacaggc attactggaa ttttagcttt tgcataatca tccttatatt
      841 tttgagcaag cgcccagaaa tgcggcggct gccagagcat catgaatata aagagaatcc
      901 atgctccgat accaaggctg ggttcaacag cagcatagcc tatcagcaca ggtagcgcac
      961 cgggaattcc tccaagaata gtaccgtaag gtgaacttcg ttttaggtag agcgtgtaaa
     1021 gaacagtgta gcttaggatt gcaagaacta ttaacagagc atttaccatg tttatgaaat
     1081 aaacagagat aaaaagtgct accgccatca aaacaattga gagtataagg gcctgtttgt
     1141 taccaacagt tttcatggct tctacccttg aaccaagtcg gtccatgagg gtatcgaggt
     1201 ccttatc
//
LOCUS       DBNGSV010000173         4826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_30497, whole genome shotgun sequence.
ACCESSION   DBNGSV010000173 DBNGSV010000000
VERSION     DBNGSV010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_30497"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..779
                     /locus_tag="AC159U_06180"
     CDS             120..779
                     /locus_tag="AC159U_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336681.1"
                     /translation="MIFYKRIFVSIVAILTISFFSVGGCDIDFSTDDSSGGSISEMES
                     MSGQIIDIIPSISIEGITAEITNTEDVTSSDSTDSSGNFFFAGDFNASPEVVTFLDSG
                     GVSLGNFSVNIFPGADVDLGDVRIDNGTVSLDFDVMVIFFGDITAINCVDNDGSIDVE
                     VDNVDVTVQIDGSTDLERESDNDNITCDDLILGQEVRVDGDMPNPTGNIVEASEVQVQ
                     D"
     gene            complement(781..2358)
                     /locus_tag="AC159U_06185"
     CDS             complement(781..2358)
                     /locus_tag="AC159U_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR3336682.1"
                     /translation="MSNSEEFKKVIDKGYTFKGDYITLGGAMYEGQCIKDTLVKVPLK
                     TLNRHGLIAGATGTGKTKTLQVLAENLSDKSIPVLLMDLKGDLSGLARSSAGHPKIDE
                     RHEQIGISWNPSEYPVELLTISKQNGVRLRSTVSEFGPVLFSKILDLNDTQTGVVSLI
                     FKYCDDNGLPLLDLKDFKKAVQYITNEGKKEIEKEYGRISTASTGAIIRKIIEIEQQG
                     AELFFGEKSFEVDDLLKIDQKGRGKINILRLTDIQDKPKMFSTFMLSLLAEIYSSFPE
                     EGDLDCPKLVIFIDEAHLVFDQASSALLEQIEMIIKLIRSKAVGIYFCTQTPRDIPDS
                     VLSQLGMKVQHALRAFTAKDRKTIKMIAQNYPDSKFYDTNKVLTSMGIGEALITVLNE
                     KGIPTPLAATLLRAPKSRMDILSNSEITQTIDSSRLVKKYNETLDRESAYEMLERKIL
                     DFKEEEKQKEIKKQQVKIEKQRKKESKSSSIINSTFGRQIGRTVAREVTRGILGVLGL
                     GGKTRRRRRKKPSFSWF"
     gene            2495..2899
                     /locus_tag="AC159U_06190"
     CDS             2495..2899
                     /locus_tag="AC159U_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HIR3336683.1"
                     /translation="MAKITEVEGIGPTYGGKLSDAGIKTQETLLKEGGTKKGRKEIAE
                     NTGISEKLILEWVNQADLARIKGVGSEYSDLLEASGVDTVPELAQRNAANLHKKMEEV
                     NTDKKLVRILTSESQVEDWVKQAKSLPRAVHY"
     gene            complement(2900..3964)
                     /locus_tag="AC159U_06195"
     CDS             complement(2900..3964)
                     /locus_tag="AC159U_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3336684.1"
                     /translation="MRNKNTITISKELIETPDERELQNTLQIRWFGTSNYLISYGNNS
                     IMTDPFVSYQNLSNVVFGNSIKSNDTYISKYYEKIITPDAIFIGHSHYDHLMDTVPLL
                     KHKKDESGCNVPVYGSITTKNILHGYKNECGCSNGDDSKRWSYNSQIVETLGNWKSVE
                     TMNCKRQTRGSNSDIHYKAYIADHANHLELGPLKFKLFEGDEKNPLEYPPNIAYDFRE
                     GDTYLYIFKLIKDSKSVIIAIAGAATNVTASKENPENQVSQVTDTVDVLILCVPGWKY
                     KEDYPGKLIDILRPKHILLSHFDNFFDSNRHKEVIEDVPLSDLDKFILHLQDDIESID
                     DYERFENIIIPDVNTTVYLQ"
     gene            complement(4105..>4826)
                     /locus_tag="AC159U_06200"
     CDS             complement(4105..>4826)
                     /locus_tag="AC159U_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3336685.1"
                     /translation="FWEKPEIKKHQLLKSNFDVDLQEYPINIIAIGNDSMLRQGILNV
                     LDADKSIKIRCQVSSKLEILECSYSNDIDLAIILDDSNYSDELFDIIKFLKNEVSDVK
                     ILVINPKPDDKNELRLIENTVSGVVSYKEDNVNIPLTIHSIMNGDLWYRRDILNNYVK
                     QKNTNINIKITANDKTFDMFSLKEKQIIAFASQGYTNKDIGDKLSIAEGTVKNYLYRI
                     YKKLNIHNKKDLKAYSLKYFD"
BASE COUNT         1466 a          808 c          783 g         1769 t
ORIGIN      
        1 atttttagta ttcttcataa taatttttaa attattagca atttaacatg aaatattctt
       61 ttccaaaaca atattttaag ttatacttta actttgagga ctaacatagg agattgatta
      121 tgatatttta taagagaatt tttgtatcta tagtagcaat tttaactata tctttttttt
      181 cagttggcgg ttgtgatata gattttagca ctgatgattc cagtggaggc agtatttctg
      241 agatggaatc tatgagtgga cagataattg atattattcc atcaattagt attgaaggga
      301 ttacagcaga aataactaat actgaggacg taacctccag tgatagcaca gattcatcgg
      361 gcaacttctt ttttgcagga gattttaacg caagcccgga agtagtaaca ttcttagact
      421 ctggaggcgt tagccttggt aacttcagtg taaatatatt ccccggagca gatgttgatc
      481 ttggagacgt tagaattgac aatggaacag tgtctttgga ttttgatgtc atggtaatat
      541 tttttggaga tataactgca ataaactgtg ttgataatga cggatcaatc gacgtagagg
      601 ttgataatgt agatgtaaca gttcagattg atggctctac agatttagaa agggaaagtg
      661 acaatgataa tataacctgt gatgatttaa tattaggaca ggaagtaaga gtagacggtg
      721 atatgccaaa tcctacagga aatatcgtag aggcttccga agtacaggtc caggactaat
      781 ctaaaaccag ctgaaactcg gttttttcct gcgcctcctt ctggttttgc cacccaggcc
      841 tagtactccc aatatgcctc tggttacttc tcttgcgaca gttctgccta tctgtcttcc
      901 aaaagtactg tttataatac ttgagctttt agattccttt tttctttgtt tttcaatttt
      961 tacctgctgt ttttttattt ctttttgttt ttcttcttct ttaaagtcga gtatttttct
     1021 ttctagcatt tcatacgcac tttctctgtc cagtgtttca ttgtattttt taaccagtct
     1081 agatgaatct atggtttgag ttatttcaga attactaaga atatccattc tagattttgg
     1141 tgcccgtagt agtgtggcgg caagtggagt cggaatacct ttttcattta gaactgttat
     1201 aagagcctct cctattccca tagaagtaag caccttgttg gtatcataga attttgaatc
     1261 cggatagttt tgtgcaatca ttttaatagt ctttctatct tttgcagtaa atgcccttag
     1321 tgcatgctgt actttcattc caagctgact tagtacacta tctgggatgt cccttggagt
     1381 ttgagtacaa aaataaatgc ctacagcttt tgatcttatt aactttatga tcatttcaat
     1441 ctgttctaac agtgcgcttg atgcctgatc aaaaaccaga tgggcttcat ctataaatat
     1501 cacaagcttt ggacaatcaa gatcaccttc ttcaggaaaa cttgaataaa tttctgctag
     1561 aaggctaagc ataaaagtag aaaacatttt tggtttatcc tgtatatcag taagtcttag
     1621 tatattaatc ttgccccttc ccttctgatc gatctttaat aggtcatcta cctcaaacga
     1681 tttttctcca aaaaacagct ctgccccctg ctgttctatt tctataatct ttcttattat
     1741 tgctccggtt gacgctgtgg atattcgccc gtattctttt tctatttctt ttttgccctc
     1801 atttgttata tattgaactg cttttttaaa gtcttttaaa tccagcagag gaaggccatt
     1861 atcatcacag tacttaaaaa ttaaagaaac aacacccgtc tgcgtatcat ttagatcaag
     1921 tattttagaa aatagaacgg ggccgaattc tgagacagta gatctaagcc ttactccatt
     1981 ttgtttggat atggtcaata actccacagg atattcactt ggattccatg atattcctat
     2041 ttgttcgtgt ctttcatcga tttttggatg accggcgctt gaccttgcaa gaccactaag
     2101 atcacctttt aaatccataa gtaataccgg tatgctttta tccgataaat tttcagcaag
     2161 cacctgaagt gttttagttt tacccgttcc ggttgcaccg gctataagcc catgtctgtt
     2221 tagtgtctta agcggaactt taacgagagt atcctttata cactgcccct catacatcgc
     2281 accaccgagt gttatataat cccctttgaa tgtatagccc ttatcaatta cttttttaaa
     2341 ctcttcagaa ttactcataa tttatatata gactcccttg caaaataagg tttatattaa
     2401 aattactgat attttatgtc aattaatata attttatttt gttattaaat tttattgatt
     2461 ataattatta aaatttttca gatggaggaa attgatggct aaaatcacag aagtagaagg
     2521 aattggacct acatacggcg gcaaattatc tgatgcagga attaaaactc aggaaacact
     2581 tctaaaagaa ggtggtacta aaaaaggcag aaaagaaatt gccgaaaaca ctggcataag
     2641 tgaaaaacta atacttgaat gggttaatca ggcagacctt gccaggatta agggagttgg
     2701 gagtgaatac tctgacctgc tcgaagcatc cggagttgat actgttcctg agctagctca
     2761 aagaaacgct gcaaatttac acaagaaaat ggaagaagtt aatacagata agaaacttgt
     2821 aagaatacta acttcagaat cacaggtgga agattgggtt aaacaggcaa aatctcttcc
     2881 aagagccgtg cattattaat tactgcaaat agacagtggt gttaacatcg ggaattataa
     2941 tattttcaaa tctctcataa tcatctattg attcaatatc gtcttgtaaa tgtagtatga
     3001 atttatccag atctgataaa ggtacatcct caataacttc tttatgccga tttgaatcaa
     3061 aaaagttatc aaaatgagag agtaatatat gttttggtct taagatatca ataagtttgc
     3121 caggataatc ttctttgtat ttccatcccg gaacacaaag tattagcaca tctaccgtat
     3181 ctgtaacttg agatacctga ttttcaggat tttccttact cgctgttaca tttgtagcag
     3241 cccctgcaat agctataatg accgatttgg aatcttttat gagtttaaaa atgtacagat
     3301 aagtatctcc ctccctaaaa tcatatgcaa tattaggtgg atactctaga ggatttttct
     3361 catccccttc aaataattta aattttaacg ggcctaattc tagatgattt gcgtgatcgg
     3421 cgatataagc tttgtagtgg atatcagaat tacttcctct agtttgtctt ttgcaattca
     3481 tagtctctac cgatttccag ttacctagag tttcaacaat ttgggagtta taagaccatc
     3541 ttttactatc atcaccgttt gaacaaccgc attcattttt atatccatgc aagatattct
     3601 ttgttgtaat actcccataa acaggaacat tacatcccga ttcatctttt ttatgtttta
     3661 gtaatggaac tgtatccatt aaatgatcat aatgggaatg tccgataaaa atagcgtctg
     3721 gtgttattat tttttcataa tatttactta tataagtatc gtttgatttt attgaatttc
     3781 caaaaactac atttgataaa ttctgataac tcacaaaagg atcggtcatt atagaattat
     3841 tgccatagga tattaaataa tttgaagttc caaaccacct gatttgcaaa gtgttttgta
     3901 attctctttc atcaggcgtt tctattagtt ctttagaaat agtaatagta tttttattcc
     3961 tcacagggat tggttttgtg caggaataaa tgttaacgat gaaaattaat ataactaaga
     4021 tatgtatagt gtttatatac atgtaaatta caatgtaatt acctaaccat attatttctt
     4081 gtactttttg tttattaata tcttttaatc aaaatatttt agagagtaag cttttaaatc
     4141 ctttttattg tgaatattta gtttcttgta tattctgtat agataatttt ttactgtacc
     4201 ttctgctata gagagtttgt cacctatatc cttgtttgtg taaccctgtg aagcaaatgc
     4261 gattatttgc ttttctttaa gagaaaacat atcaaatgtc ttatcatttg ccgttatttt
     4321 tatatttata tttgtatttt tctgtttaac ataattattt agtatatctc ttctatacca
     4381 cagatctcca ttcatgatag aatgtatcgt caaagggata tttacattgt cttctttgta
     4441 gctaactaca ccgcttactg tgttctcaat aagtcttagt tcatttttat catcaggctt
     4501 tgggttaatt actaatattt tcacatctga aacttcattt tttaagaatt ttatgatatc
     4561 aaatagttca tcagaataat tagaatcatc caagattatt gctaaatcaa tatcattaga
     4621 atagctacat tctaatatct caagttttga agagacctga caacgtattt taatagattt
     4681 atcggcatct agtacattta aaatgccttg tcgtaacata ctgtcatttc caatggcaat
     4741 aatatttatt ggatattctt gtaaatcaac atcaaaatta gattttaata gctgatgttt
     4801 tttaatttca ggtttttccc aaaaga
//
LOCUS       DBNGSV010000174         3641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1276884, whole genome shotgun sequence.
ACCESSION   DBNGSV010000174 DBNGSV010000000
VERSION     DBNGSV010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1276884"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            571..2034
                     /gene="dnaA"
                     /locus_tag="AC159U_06205"
     CDS             571..2034
                     /gene="dnaA"
                     /locus_tag="AC159U_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3336686.1"
                     /translation="MQKKLEAKADSDLISGTNTENYTNHLSSVHEHSEQAVDWEQVLE
                     TIRKKSNPQVFFWFSPLKVVTQNSKQLILKANSSFDKDWINNHYMDFIVDSIREIYHN
                     DIEVKIICESEQAESAKAADNFKSRNKSIDDIALPKNTFFTRSLNPNYSFENFIVGPS
                     NQFAHAASLAVARKPGQSYNPLFIYGGVGLGKTHLLNAVGNFISAASSNHSRVCSISA
                     EQFTNEVINSIKSNKMEEFRNKYRYGCDVLLIDDVQFIAGKESTQEEFFHTFNTLYES
                     KKQIILTSDKSPRDMSYLEERLRSRFQWGLITDIQPPETETRIAILKKKAESEQVSLP
                     DEVANFLAQNIISNVRELEGTLTNIIGYAKLLNSQISIDLAKEVLKNILKNDDRKVLS
                     IESIQKEVSNYFGIKTNDLKSNKKQKNIAFPRQISMYLVRRYTGASFPEIGEKFGGKD
                     HSTVIHAVNKIERLLKDDLSVKNTINSLSRTMESLRG"
     gene            2255..3361
                     /gene="dnaN"
                     /locus_tag="AC159U_06210"
     CDS             2255..3361
                     /gene="dnaN"
                     /locus_tag="AC159U_06210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3336687.1"
                     /translation="MKFVCDASSLSEKLSIVQGISERRITMPILSHVLISSNENNITV
                     TATDLENTLQTECSAIVQASGKIALPSKKLFEIIREIHSGDIEIEELDNNWVQIQSSG
                     VNFKLAGLPAADFPSLPVQQLQNQADVQAAMLDDMIAKTFFAVSADDLRRNLAGLYLE
                     PLDGGMLRIIGTDGHRLSYIEKKFESSLGFNTGIILPRKGVSELRKILKNTDSGQVKL
                     TVMNNFFIAESKDITLFSRLIELEYPDYKQVVPEVSKYSFVVNRETLKTAVRRVSVLS
                     SEKTKSIKMNIGPSNLQLLSVTPELGEAKEQIEVQYTDEPVELGFNSVYIMDVLDAID
                     SEQVEIEITDELSPALIRPGGDQNYVSVIMPMRV"
     gene            3393..>3641
                     /locus_tag="AC159U_06215"
     CDS             3393..>3641
                     /locus_tag="AC159U_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3336688.1"
                     /translation="MIDILFPTPCLLCGNELKNSIVCSRCFSLFNFINNTEVCEKCGV
                     PFSGFNEKSARLCGGCIENKDSMLAVRSVLLYKDNLKEL"
BASE COUNT         1359 a          591 c          655 g         1036 t
ORIGIN      
        1 tatattatta agctagtaaa tttagtacat aaatttaata cttaaaatat ttaaattatt
       61 cataaccgga ataagagaaa atcagtgaga taatatattt aaaaacaaat ttaataacaa
      121 tcaaaaatat tctttatagg ttaaaatcac aaccaaaaac aacacttatt taatgtaaaa
      181 aattaacaaa catgatacat aacctaaact ttcattctct aagtagcctc acttattaca
      241 attacgataa acatagcata tttaataagc tgatagtttg aataataaat aagaaatata
      301 tattaatctc aataatataa tatatatagt gtgataatat accaagcatc tgtttgatat
      361 aactgatagt tttgtttata agtcatacta aatactcgac ttaaaaaagt ttcttgacaa
      421 cggtttttcg aatctgttac gattaagttc tatcttttac ggggtgttat aatttatgca
      481 gttttcattt aagaattctt tactgtgtaa aatggagtta agaaatagtt gaggggtttt
      541 acagataccg ttttatcttg aggaggagta gtgcaaaaaa agctagaagc caaggctgat
      601 tctgatttaa tatcaggtac caacaccgaa aattacacca atcatttatc aagcgttcac
      661 gaacactccg agcaagctgt agactgggaa caggttcttg aaaccataag aaagaagtcc
      721 aatccacagg tgtttttttg gttttctcct ttaaaagtag tcacacaaaa ctcaaaacag
      781 ctaatcctaa aagcgaacag cagcttcgac aaagactgga taaataatca ttacatggat
      841 ttcatagtag atagcataag ggagatatat cataacgata tagaagtaaa gatcatatgt
      901 gaatcggagc aagcagaatc tgctaaagct gctgataatt ttaaatctag gaacaaatca
      961 atagatgata ttgcattacc aaaaaacact ttttttacaa gatctctcaa tccaaactac
     1021 tcatttgaaa attttattgt aggaccaagc aaccagtttg cccatgcagc gtctcttgct
     1081 gtagcgcgaa aaccaggaca gtcatataat ccgcttttta tatacggcgg tgttggactt
     1141 ggaaaaacac atctgctaaa tgcagtagga aacttcatct cggcagcttc ttcaaatcac
     1201 tcaagagtgt gcagtatatc tgccgagcag tttacaaatg aggttataaa cagtatcaaa
     1261 tcaaacaaga tggaagaatt tagaaataaa tacaggtatg gctgtgatgt tttactaata
     1321 gacgatgtac agtttattgc aggaaaagaa agcacacagg aagaattttt ccacacattc
     1381 aacacactct acgaatctaa aaaacagata attcttacaa gtgacaaatc cccaagagat
     1441 atgtcttatc ttgaagaacg actgcgctcc aggttccagt ggggtcttat aaccgacatc
     1501 cagccccctg aaacagaaac aagaatagct attttgaaga aaaaagcaga gtctgaacag
     1561 gtctccctgc ccgatgaagt agcaaatttt cttgctcaga acatcatttc taatgtaagg
     1621 gagcttgaag gaacacttac aaatataatt ggctacgcca aactgctaaa ctcacaaatt
     1681 tctattgatc ttgcaaaaga ggtgcttaaa aacatcctta aaaacgatga cagaaaagta
     1741 ttaagtattg aatccataca aaaagaggtt tcaaattact ttggaattaa aaccaatgat
     1801 ctaaagtcta ataaaaaaca aaaaaatatt gcctttccaa ggcaaatttc aatgtacctt
     1861 gtaagaaggt acacgggtgc atcttttcct gagatcgggg agaagttcgg aggtaaggat
     1921 cattccactg taatacatgc tgtaaacaaa attgaaagac tgctcaaaga cgacctgtct
     1981 gtgaaaaaca ccataaattc tctatccaga actatggagt ctttaagggg ttgatattat
     2041 gtggataaaa tgttaattaa caactaagtt gttttgaata tgggattgcc tgtatataga
     2101 tgtttataaa gtaaagtatt tatcaacagt ttagtaacaa taaaatgtgt gtaattagaa
     2161 tagcttatag tactaataaa caatttcaca gtgcctgtta ctactacttc tataattaaa
     2221 ctatatatat atataataat taggagtgta aataatgaag tttgtatgtg atgcatcgag
     2281 tttatctgaa aaattatcaa tagtgcaggg aatctccgaa agaagaataa ctatgcctat
     2341 tctttcacac gtgcttatta gttcaaatga aaacaatata acagtaacgg ctacagacct
     2401 agaaaacacc ttacaaactg agtgcagtgc cattgtacaa gcaagcggta aaattgcact
     2461 tccttcaaaa aaactgtttg aaataataag agaaattcac agcggagata ttgaaattga
     2521 agagcttgat aataactggg tacagataca aagctcagga gtaaacttca aacttgcagg
     2581 acttccagca gcagacttcc cgtctctgcc agtacaacaa ctccaaaatc aggcagatgt
     2641 acaggctgca atgctcgatg atatgatagc aaaaactttt tttgcagttt cagcagatga
     2701 cctgagacga aaccttgcgg gtctttactt agagccgctg gatggcggga tgctaagaat
     2761 tataggaaca gacgggcaca ggttatcata catagaaaag aagttcgaaa gctcgctggg
     2821 gtttaatact ggaataatac ttccaagaaa aggagtttca gagcttagaa agattttaaa
     2881 aaacacagac agtgggcaag taaaacttac agtaatgaac aattttttta ttgcagagtc
     2941 taaggacata actctgtttt caaggcttat agagcttgaa taccctgatt ataaacaggt
     3001 tgtaccagaa gtatcaaaat attcatttgt agtaaacagg gaaacactta agactgcggt
     3061 aagaagggta tcggtgcttt cttcggaaaa aacgaagagc ataaaaatga acataggacc
     3121 ttcaaactta cagctacttt ctgtaacccc ggagcttggg gaagcaaaag agcaaataga
     3181 ggtgcaatac acagacgaac cagtagaact tggatttaat tctgtatata taatggatgt
     3241 gcttgatgca atagactctg agcaggtaga aatagaaatt acagatgaat taagcccggc
     3301 gcttattaga cccggcggag atcagaacta tgtttcagtt ataatgccaa tgagggttta
     3361 acctttctat aacaataact ttctaaggtt caatgataga catactgttt cctacaccat
     3421 gtctgctatg tggaaatgaa ctaaaaaata gtatagtatg tagcagatgc ttttcgttat
     3481 tcaattttat caataacact gaagtatgtg aaaaatgtgg ggttccattt tcaggattta
     3541 acgaaaaaag tgcaagactt tgcggcggat gtatagaaaa taaagacagt atgttagcag
     3601 taaggtcagt cctgctttat aaagataatt taaaagagct a
//
LOCUS       DBNGSV010000175         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_723548, whole genome shotgun sequence.
ACCESSION   DBNGSV010000175 DBNGSV010000000
VERSION     DBNGSV010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_723548"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="AC159U_06220"
     CDS             <1..1794
                     /locus_tag="AC159U_06220"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR3336689.1"
                     /translation="DGGKTVNSLRLFKAQSPVKLDFEKFYFGDFKTAAVRNIYAQNIS
                     KILYPSDSNEKGKELRLLQEYFLVSCSIQDILRKFEKRGETYENLPEIIAIQINDTHP
                     SLAIPELMRLLLDEKYIEWDKAWEITTKTISYTNHTLLPEALERWPVYLMERMLPRHL
                     DIIYKINKHFLSKVEHIWKGNLDKLRSMSIFEESETKYVRMANLSIIGSHSVNGVSQI
                     HSELVKTTLVPDFYQLWPQKFNNKTNGITQRRWLLHSNPLLAELITSKIGDKWITDLS
                     RLSELEQYSTDEEFQNRVLEIRNKNKSRLVEHIQRQLGIVVMKESIFDAQIKRIHEYK
                     RQLLNVLNIIHMYLCITEDGLEPQNPHTFIFAGKSAPSYYTAKLIVKLINSVAKTINN
                     DPKTKDLLKVIFIPNYNVSLAELIIPAADVSEQISTAGMEASGTGNMKFALNGAIIIG
                     TLDGANIEIKDEVGQDNIYIFGKTAEEINKYKQEKSYKSREYYEKNTNIKRVLNTLIT
                     DLFSPGDPRLFKPLFDTLIHHGDRYFLLTDFDSYISAHKLIDSDFGNKSQWLKRSILN
                     TSRSGKFSSDRTIREYADDIWKIKSLSEYEN"
BASE COUNT          665 a          343 c          375 g          529 t
ORIGIN      
        1 gatggaggga aaacagttaa cagcctgagg ctttttaaag cccagtctcc tgtaaaactt
       61 gatttcgaaa aattctattt cggagatttt aagacagcag cagtgaggaa tatttatgcc
      121 caaaacataa gcaaaatcct ttatccctcg gattcaaatg aaaaaggcaa agagttaaga
      181 cttcttcagg agtattttct ggtttcttgt tccatacaag atattttaag aaagtttgaa
      241 aagagagggg aaacttatga gaatctgcct gaaataattg ctattcaaat caatgatact
      301 catccttcac ttgcaattcc agaactcatg aggctgttgc tggacgaaaa gtacatagaa
      361 tgggataaag catgggaaat cactacaaaa accatatctt acactaacca tactcttcta
      421 cccgaggcac tggaaaggtg gcccgtgtat cttatggaaa gaatgcttcc caggcatctg
      481 gatatcatct ataagatcaa caagcatttc cttagtaagg tcgagcatat atggaagggt
      541 aatttagata agctgagatc aatgtcgata tttgaagaga gtgagacaaa gtatgtgaga
      601 atggccaatc tttccataat aggtagccac tcggtaaacg gtgtctcgca gattcattcc
      661 gagcttgtaa aaacaacgct tgttccagat ttttatcaat tatggcctca aaaattcaat
      721 aataagacaa acggtatcac acagcgcaga tggcttcttc attcaaatcc gcttcttgcc
      781 gagcttataa catcgaaaat tggtgacaag tggataactg atctatcaag actgagcgag
      841 ctagaacagt actctactga tgaggaattt caaaaccggg ttttggaaat cagaaataaa
      901 aataaatcca ggcttgttga acacatacaa aggcagttag gcatagtagt tatgaaggaa
      961 tcaatatttg atgcacaaat caagaggata catgagtaca aaaggcagct tctaaatgtt
     1021 ttgaatatta tccatatgta cttatgcata acagaagatg ggcttgagcc gcaaaatccg
     1081 cacactttta tatttgctgg taaaagcgcg ccatcatact atacagctaa gcttattgta
     1141 aagcttataa acagtgttgc caaaacaata aacaacgacc cgaagacaaa ggatttatta
     1201 aaagtgattt ttattcctaa ctataatgtt tcacttgctg aacttatcat tcccgcagcg
     1261 gatgtaagtg aacagatatc cacagcaggc atggaggcat ccggcacggg aaatatgaag
     1321 tttgcgttaa acggggcaat aattatcggc acccttgacg gtgcaaacat agaaatcaaa
     1381 gatgaagtcg ggcaggataa tatatatatc tttggtaaaa cagctgagga gattaataaa
     1441 tacaaacaag agaagagcta taaatccagg gagtattatg aaaaaaacac aaacataaaa
     1501 agagtgctca atacgctcat tactgatctg tttagcccgg gtgatccaag actgtttaaa
     1561 ccactttttg atactttaat acatcatgga gacaggtatt tcctgttgac tgattttgat
     1621 tcgtatatat cagcacataa gctaatagat tctgatttcg gcaataaatc acagtggctt
     1681 aagaggtcta tactcaatac aagccgatct ggaaaatttt ccagcgatag aacaattaga
     1741 gaatatgctg atgatatatg gaaaattaaa agtctgtcgg aatatgaaaa ttagacgata
     1801 actacagagt cttcagaacc atattcgttg ggcacgtaat tgtccgcatc ccagtcgttt
     1861 tcccatttgt cgccgtctat tacatatttg aatttatact catttccgga tt
//
LOCUS       DBNGSV010000176        11876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_169617, whole genome shotgun sequence.
ACCESSION   DBNGSV010000176 DBNGSV010000000
VERSION     DBNGSV010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_169617"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="AC159U_06225"
     CDS             complement(<1..662)
                     /locus_tag="AC159U_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3336690.1"
                     /translation="MYIAAIDLGTNSFRLLIAKIELNQIIFVQKTSIITGLGKDFDTN
                     NKLLSKKSISRSLKVLKKFSLTLKRFQISSLRAVATSVVRNSDNSQDFIKQASSILNT
                     KIEIISGKKEAELTTIGVLNSVNITPGNKIVVDIGGGSTEFGIVDENEKINFECSINL
                     GVVSIAEKFNLRKPISGEVTFTLQTEPGKSREIWQNNAINQEVFCHEEVKIYRTTDSI
                     RF"
     gene            complement(677..1192)
                     /locus_tag="AC159U_06230"
     CDS             complement(677..1192)
                     /locus_tag="AC159U_06230"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3336691.1"
                     /translation="MICAFNNKHPNIHDSVFIAKSAQVIGQVSIGKSSSVWFNSVVRG
                     DVNYIEIGQRTNIQDNSVLHVTKNTYPLVIGNNVTIGHRVVVHGCSIEDNTLIGIGSV
                     LLDGSVVNSNSIVAAGSLVPPGFNIPSGVLAVGSPVKIKRDLNEQELTLIDKLSNNYV
                     EYSLQYLSQDF"
     gene            complement(1214..3292)
                     /gene="fusA"
                     /locus_tag="AC159U_06235"
     CDS             complement(1214..3292)
                     /gene="fusA"
                     /locus_tag="AC159U_06235"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3336692.1"
                     /translation="MSSDYPRENIRNITLVAPHGAGKTSLAEGLLYINGSLDKLGKVD
                     NGSTYLDAEAEEKNRQMTINTQVGYYDHKGFNVNVIDTPGFPNFLYQTELAVDITDTA
                     VLLVSGLADSKEQIDRFWDIIKSRNVSSLIFMNKLDKERADFEQSLNHLKDTLKINPV
                     VLSVPIGSEQNFSGVVDLLSMKSYRIDGTKSIIGDIPDDCKELSEKYREKLIESIAEN
                     DDDLLERYLNGDELTDEELQNTLKKAVIDGNAIPLVVGSATELTGLDYLCQTINNILP
                     GPLDRKKINIIKGEEQSDLKPLVDGPVVGSVFKTISDPYAGKISILRIFSGSVNSDAT
                     LINSSDGSKVKIGQISKILGKRLSNVDKAQCGDIVAVNKIKELHTAQTLTSDSEAIKV
                     LLSTPPHAVLSYAIEPQSRGDEDKLMPSLSKIAEEDTTLDYHRDEETNDFLLSGTGQM
                     HVEVIVERLKNVYGVTVDLKTPKIPYKETIKKSAKAEGKYIKQTGGRGQYGDAWIQID
                     PLTNGQDFEFVDKIVGGVIPRNFIPSVEKGVKDAMKKGVYAGYPVTGVKVTVFDGKYH
                     PVDSSDVAFQIAGSMGFKKAMEEAKPTLLEPIMKMKVFTPEEVMGDVIGDINSRRGKV
                     SGMDTASGGSEINANVPLAEILTYAPELRSITSGRGNFSVEFSHYEEVPAQIAQKIIQ
                     QQNSSESTDD"
     gene            complement(3439..3642)
                     /gene="rpmE"
                     /locus_tag="AC159U_06240"
     CDS             complement(3439..3642)
                     /gene="rpmE"
                     /locus_tag="AC159U_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3336693.1"
                     /translation="MKAEIHPEYKEVTITCACGNVITTRSTKTEDFGIDICSNCHPYY
                     SGKERSTESKGQVERFRRKFGNK"
     gene            complement(3663..5600)
                     /gene="dnaK"
                     /locus_tag="AC159U_06245"
     CDS             complement(3663..5600)
                     /gene="dnaK"
                     /locus_tag="AC159U_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3336694.1"
                     /translation="MAKSVIIGIDLGTTNSVVSIVEAGEPKVIINDEGSRITPSVVAI
                     SKDGEILVGNPAKRQAVVNPENTIYSVKRLMGRRFDETIEEAKKLPYKVIKSKNDDAW
                     VEVDGKEFSPPQISSHVLSKLKAAAETYLGEKVTEAVITVPAYFNDSQRQATKDAGKI
                     AGLEVKRIINEPTAAALSYGFDKKKEGMVVVYDLGGGTFDVSVLEIGDNVIEVKSTNG
                     NTSLGGDDFDRTVMDHIIGEFKKDQGIDLSNDNMALQRLREAAEKAKIELSSTMETEV
                     NLPFITADASGPKHLVLKITRSKFEQLTESLIIGTLEPCKQALKDAGLKASEITEVIF
                     VGGSTRIPMVHKVVTDFFGKEPNKSINPDEVVALGAAIQGAVLGGDVRDVLLLDVTPL
                     SLGIETLGGVMTPIITRNTTIPTKKSQIFTTAEDNQTSVEIHVLQGERQMASDNRTLA
                     KFILDGLPPAPRSVPQIEVTFDTDADGILHVSAKDQATQKEQKIKVEASSGLSKDEID
                     QMVKDSEDKAEDDKERKELIETRNKLDELIYRTDKSFKELGDKLSEEEKKELEDSLEQ
                     GKTALKGDNKEELESALERITKSSHKLAELMYKQASENSDQPADQSAEEQTEQTAESD
                     IPTDESNKKDDDVIDAEFTEK"
     gene            complement(5715..6875)
                     /gene="sucC"
                     /locus_tag="AC159U_06250"
     CDS             complement(5715..6875)
                     /gene="sucC"
                     /locus_tag="AC159U_06250"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3336695.1"
                     /translation="MNIHEYQAKELLAKNGMNVPAGKVATSSEEAMQIANDLQSDGFV
                     VKAQIHAGGRGKGGGVKLASTTEDVGRLASDIIGMNLVTHQTGPEGKLVGKVLVEEAS
                     QIEKEFYLGMVIDRSKEKIVVMASPEGGVEIEEVAKHSPEKIIKEYVDISTGLLPFQG
                     RKIAYFLGLKGKTANKAVNLIVRLYEVFVNSDCSLAEINPMILTKSDDVIPLDAKLNI
                     DDNALYRHKDLQAYHDPTEEDPTELEAKEWGISYVKLDGNIGCLVNGAGLAMSTMDII
                     KYHGGEPANFLDVGGGATPEQVTQAFKMIVSDNNVKAIFVNIFGGIMKCDTIATGIIS
                     AAKEVGIEVPLVVRLEGTNVELGREMLADSGLNIITGKDMKECAAKVVEAAQ"
     gene            complement(6909..7646)
                     /locus_tag="AC159U_06255"
     CDS             complement(6909..7646)
                     /locus_tag="AC159U_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3336696.1"
                     /translation="MADEAVLRVFRGDSEKGETVDYKVPVEEGMVVLDALHYIQANFD
                     GDLAVRWNCKAAKCGSCSTEINGMPKLSCKTRMDSLSHDEPITVYPIKAFPVIKDLVT
                     DVKWNFQKHKEIPPFTPPEGEEELTMYQEDVERSQEFRKCIECFMCQNVCHILRDNNA
                     KDAFFGPRYMIKIAELDMHPLDTVDRSELLKEEAGVAYCNITKCCTEVCPESIHITDN
                     AIIPLKERVVDKYFDPINWILSKFKEG"
     gene            complement(7646..9370)
                     /locus_tag="AC159U_06260"
     CDS             complement(7646..9370)
                     /locus_tag="AC159U_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3336697.1"
                     /translation="MDKYETHEHDVIVIGAGGAGLRAAIEASAQGASVGLVCKSLLGK
                     AHTVMAEGGVAAAMANVAPEDNWQTHFKDTMKGGKMLNNWRMAQIHATEAPDRIHELE
                     QWGALFDRTKEGKINQRAFGGHTWKRLCHVGDRTGLELIRTLQDKGIHSGIDVYMECT
                     ITDLVKDGDKVVGAFGYWRETGKFVLFKSKAVILATGGIGKIYQVTSNSWEYTGDGQA
                     LAYNAGAELIDMEFIQFHPTGMVWPPSVKGILVTEAVRGEGGILTNSKGERFMEKYDP
                     ERMELSTRDVVARSIYTEAQEGRGTPHGGAYLDISHRGAEYVIKKLPSMYHQFMEFAD
                     IDITKDPMEVGPTTHYVMGGVRVDPETGQSTVPGLFAAGEVAAGMHGANRLGGNSLSD
                     LLVFGRRAGLGAAEYANEIDANTLNMNYGQITQHEKHILAPFSNTNGKNPYTVHQNLQ
                     EIMQNYFGVFRIKDKLEQGLKELEALINDSQVLSVEGSRMFNPGWNLSVDLNSLFIVA
                     EAAAKCALQREESRGAHSRVDFPESDNTKWNKVNSVISKDGDKMNLGLSPLPEMPDEL
                     KSLFSEDS"
     gene            complement(9373..10101)
                     /locus_tag="AC159U_06265"
     CDS             complement(9373..10101)
                     /locus_tag="AC159U_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR3336698.1"
                     /translation="MGSSASQNYTTLRTDNWKTAPMITAAVLGSFVLYATWRALENQY
                     YLAEPYLSPFYSPLINPEWWPLSPAILILWAPGLFRFTCYYYRKAYYRSFAGMPPGCA
                     VEGRSKESYNGESKFPLILQNLHRFFFYAATIVLIFLWYDAYKGFKFEDGYGVGIGSV
                     VLVINAFLLSMYSLSCHSFRHLIGGKLNIFSACPTRFSLWKGASSLNENHMLWAWLSL
                     FGVCLTDLYVRLVATGVINDVRFI"
     gene            complement(10193..11134)
                     /gene="mdh"
                     /locus_tag="AC159U_06270"
     CDS             complement(10193..11134)
                     /gene="mdh"
                     /locus_tag="AC159U_06270"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3336699.1"
                     /translation="MSRPKISVIGAGNVGATAALRLAESELGDVVLVDIVEDMPQGKA
                     LDLMETGPVVKYDVNITGSNGYDETADSDIVIITSGLPRKPGMSRDDLLATNTKIVTQ
                     VTTEVAKKSPNAILILVTNPLDAMTYVAYKASGFPRERVLGMAGVLDSARFRTFISME
                     LDVSVDNIQAFVLGGHGDDMVPLARFTTVAGIPITELMPEDRVESIIQRTRDGGAEIV
                     KLLKTGSAFYAPGASAVEMAEAILKDKKKILPCCVYAEGEYGIDGTFVGLPVKLGRAG
                     IEEIIEINLTDKEKEELHISAGHVKELCDRIHELGHL"
     gene            complement(11154..>11876)
                     /locus_tag="AC159U_06275"
     CDS             complement(11154..>11876)
                     /locus_tag="AC159U_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3336700.1"
                     /translation="MKVEIIELSGIGIKPISPFGTKRLVRKAIQYAIKYNRKSVTLVH
                     KGNIMKFTEGAFRDWGYELAKEEFGNETITEDELWDKYDGKAPKDKIIIKDRIADNMF
                     QQILTRGNEYDVLAMPNLNGDYLSDALAGQVGGLGVAPGANIGDPYALFEATHGTAPK
                     YAGQDKVNPGSVILSGVMMFEYLGWTEAAELIVKAMEKTILNKTVTYDLHRQIEGATL
                     LKCSEFGQTIADNMDDIKQILI"
BASE COUNT         3257 a         2626 c         1857 g         4136 t
ORIGIN      
        1 ttaaagcgaa tgctatctgt tgttctgtaa atcttgactt cctcatggca aaatacctcc
       61 tgatttattg cattattttg ccagatttct ctacttttac ctggttcagt ttgcagggtg
      121 aaggtcactt cacctgatat aggcttacga agattaaatt tctctgcaat gcttacaact
      181 ccaagattaa tactacattc aaaatttatt ttctcatttt catctacaat accaaactcg
      241 gtactcccgc ctccaatatc tactacaatt ttatttcctg gagtaatatt tacggaattt
      301 aatactccaa ttgtagtaag ctctgcttcc tttttcccac ttataatctc tatttttgta
      361 tttagtatac tgcttgcttg ttttataaaa tcctgtgaat tatcggaatt tcttactaca
      421 ctcgtagcaa cggctctaag actacttatt tgaaaacgct ttaatgttaa cgaaaatttt
      481 ttaagtactt tcaaactacg agagatagat ttttttgaaa gtaatttatt gttagtatca
      541 aaatctttac caagacctgt aataatgctt gttttttgaa caaaaattat ttgattgagt
      601 tcaatttttg ctataagaag tctaaagcta ttagttccta aatcaattgc ggctatatac
      661 acaatataat gttgtcttaa aaatcctgtg aaagatactg aagtgaatac tctacatagt
      721 tatttgaaag tttatctatt aaggttaatt cttgttcatt taagtcgcgt tttattttca
      781 ccggtgatcc taccgcaagc actccactgg gaatattaaa gcctggtggt actaatgatc
      841 cagctgcaac aatagaatta gaatttacaa ctgagccatc aagtaatacc gagcctatac
      901 ctataagcgt attatcttca atactgcagc catgaactac aaccctgtgg ccaattgtaa
      961 cattattacc tattactaga ggataagtat tctttgtaac atgtaataca ctgttgtcct
     1021 gaatatttgt tctttgtcct atttctatgt aatttacatc ccctcttaca actgaattga
     1081 accatacgct tgaagactta cctatgctaa cttgtccaat tacttgtgcg ctttttgcta
     1141 taaatacaga atcatgaatg tttggatgtt tattattaaa agcacaaatc atatacttaa
     1201 gtaatatcta gaattaatca tctgtagatt ctgatgaatt ttgttgttgt attatttttt
     1261 gtgctatctg agcaggtact tcctcataat gtgaaaattc gacgctaaag tttcccctgc
     1321 cacttgttat agaacgtagt tcaggagcat acgtaagtat ttctgctaaa ggcacatttg
     1381 cgtttatttc acttcctcca gacgctgtat ccatgcctga taccttaccc cttctggaat
     1441 tgatatcgcc tatgacatcc cccattactt cttctggagt aaatactttc atcttcataa
     1501 tcggttcgag cagggttggt tttgcttctt ccatggcctt tttaaacccc atagaacctg
     1561 ctatttgaaa agctacatca gaggaatcca caggatgata ttttccatca aatactgtta
     1621 cttttacgcc tgtaaccgga tatccagcat agacgccctt tttcattgca tcttttaccc
     1681 ctttttctac agacggtata aaatttcttg gaattactcc tcctacgatt ttatcaacaa
     1741 actcaaagtc ctgcccgttt gtaagtgggt caatctgtat ccacgcatca ccatactgcc
     1801 ctctaccacc agtttgttta atatacttcc cttcagcttt agcactcttt ttaatcgtct
     1861 ctttgtaagg gattttagga gttttaaggt ctactgttac gccatataca ttttttagtc
     1921 tctctactat aacctctaca tgcatctgac ccgtacctga aagcaggaag tcatttgttt
     1981 cttcgtctct gtgataatca agtgttgtat cttcttcagc tattttagag agtgaaggca
     2041 taagtttatc ttcgtctcct ctgctttgag gttcaattgc ataagagagg actgcgtgtg
     2101 gaggagtgga tagtaatact tttattgctt ctgaatcaga tgtaagtgtt tgtgcagtgt
     2161 gtaattcttt tattttgtta acagcaacta tatccccaca ctgggcttta tcaacattag
     2221 atagtctctt gccaagtatc tttgaaattt gacctatctt taccttggaa ccatcagaac
     2281 tgtttataag agttgcatcc gagttcaccg atcccgaaaa aatccttaaa atacttattt
     2341 ttcctgcata cgggtctgat atggttttaa atacagatcc tactacaggc ccgtccacca
     2401 ggggttttaa atctgactgc tcttcacctt taataatatt tatcttcttt ctgtcaagtg
     2461 gcccgggaag gatattattg attgtctggc aaaggtaatc aagtcccgta agttcagtag
     2521 cagaaccaac gacaagaggt atggcatttc catcaattac cgctttttta agagtatttt
     2581 gtaattcttc atctgtaagt tcatcaccat ttagatatct ttccagcaga tcatcatcat
     2641 tttcagcaat tgattcaatt agtttttctc tgtatttctc actaagctct ttacaatcat
     2701 cgggaatatc accaattatt gatttagtgc catcaattct ataggacttc atgctaagca
     2761 aatctacaac tccactaaaa ttctgttctg agccaatggg tactgataat acaaccggat
     2821 ttatctttaa tgtgtctttt aaatgattta acgattgctc aaaatcagct ctttctttat
     2881 caagcttatt cataaatata aggcttgata cattcctgga tttgataata tcccaaaatc
     2941 tgtcgatctg ttctttagaa tcagctaaac cacttacaag caaaactgct gtatctgtaa
     3001 tatctacagc tagttctgtc tgatataaga aatttggaaa tcccggggtg tctattacgt
     3061 ttacattaaa acccttatga tcataatatc ctacctgggt atttattgtc atttggcggt
     3121 ttttttcttc ggcttcagca tcgagataag tagacccgtt gtctacttta cctaatttgt
     3181 ccaaagaacc gtttatatat agtaaacctt ctgcaagact ggtttttcct gctccatgtg
     3241 gggcaaccaa tgttatgttt cttatgtttt ctctgggata atcactactc atatgtacct
     3301 ctccataata gaataattac tattatagaa tcatatggca gaaaaagtaa aggtatttct
     3361 taataagatt ataaaaaaat cgtggaaaat gttaattaaa tacttatttt taataaatta
     3421 tttaatttat aacgcggctt atttatttcc aaactttctt ctaaatctct ctacctggcc
     3481 tttggactct gttgatcttt ctttaccgga atagtaggga tggcagtttg aacatatatc
     3541 tattccaaag tcttcagttt tagtagacct ggtggttata acattaccgc aggcacatgt
     3601 tatggttact tctttatatt caggatgtat ttctgctttc atttaatcaa tatacctctt
     3661 atttattttt ctgtaaactc tgcatctatc acatcgtcat ctttcttatt tgattcatct
     3721 gtaggaatat ccgattcggc tgtttgttct gtttgttctt ctgcgctttg atcagcaggt
     3781 tgatcggaat tttctgatgc ctgtttatac ataagctcgg caagtttatg agatgacttc
     3841 gtaatgcgct caagagcact ttcgagttct tccttattat ccccttttag tgctgtcttg
     3901 ccctgttcca aactgtcctc aagctctttt ttctcttctt cgcttagttt atcacccaat
     3961 tctttgaagc tcttatcagt tctgtaaatc agttcatcta acttgtttct tgtttctata
     4021 agctcttttc tttctttatc atcttctgct ttatcctcag aatccttaac catttggtct
     4081 atttcgtctt tacttagacc actggaagcc tctactttta tcttttgttc cttctgtgtt
     4141 gcctgatctt tagcagatac atgtaaaatt ccatctgcat ctgtatcaaa tgtaacttct
     4201 atttgcggta cactccttgg tgctgggggt aggccatcga gtataaactt ggccagggtt
     4261 ctattatcag aggccatttg tctttcaccc tgaagaacat gaatctctac cgaggtctga
     4321 ttgtcttcag cggtagtaaa tatctggctt ttctttgttg gaattgtagt atttctagta
     4381 attataggtg tcattactcc accaagtgtt tctattccca aagatagcgg ggttacatcg
     4441 agcagtaata catcccttac gtcaccacca agaactgctc cctgtattgc agcaccaagt
     4501 gcaacaactt catcagggtt aatactttta ttcggttctt ttccaaaaaa gtctgtaact
     4561 actttatgaa ccataggaat tctggtagaa ccgccaacaa aaattacttc agtaatctct
     4621 gaagctttta gccctgcatc tttaagagcc tgtttacagg gttcaagagt accaataatc
     4681 aatgattctg taagctgttc aaactttgaa cgggtaatct ttaagaccaa atgtttggga
     4741 ccactagcat ctgctgtaat aaatggaagg ttaacctctg tttccatagt gcttgaaagc
     4801 tcaattttag ccttctcagc agcttctcta agacgctgta gcgccatatt atcattactt
     4861 aggtctatac cctgatcttt tttgaattcg cctataatat gatccattac ggttctgtca
     4921 aaatcatcac cgccaaggct tgtattacca tttgtggatt ttacttcaat aacgttatcc
     4981 ccaatctcaa ggacagacac atcaaaggta ccgccaccaa ggtcataaac tacaaccatt
     5041 ccttcttttt tcttatcaaa accgtaagaa agcgcagctg ctgtaggttc gttgataatt
     5101 ctttttacct ctaaaccagc tatctttccg gcatctttag ttgcctgcct ctggctgtca
     5161 ttaaagtaag caggaactgt gatcacagct tctgttactt tttcacccag ataagtttct
     5221 gctgctgcct ttagtttgga aagtacatga gaagatatct gaggagggga aaattctttt
     5281 ccatctacct ctacccatgc atcatcattt ttactcttaa taactttata aggcaatttt
     5341 ttggcttctt ctatagtttc atcaaatcta cgtcccataa gtctttttac tgagtatatt
     5401 gtgttttcgg ggtttactac agcttgtctt ttagcaggat tcccaactaa tatttctcca
     5461 tctttagaaa ttgccactac agaaggggtt attctggagc cttcatcatt aataattacc
     5521 ttaggttcac ctgcttccac tatagatacc acagagttgg ttgttccaag atcgattccg
     5581 attattacac tcttagccat tgaaatgcct ccaaaatata aagatatact tatatattta
     5641 aattagatta ataaaattta aagtaatcat gaagaaaggg ttgtcaagaa gtgaatttat
     5701 aaataatttc agtattattg tgcagcttca accactttag cagcacattc tttcatatct
     5761 ttaccagtaa taatattcag gcccgaatca gccagcatct cccttccaag ctcaacattg
     5821 gtaccttcta acctaactac cagtggaacc tctataccta cttcttttgc agcagagatt
     5881 attcctgtag caattgtgtc acatttcata attccaccaa aaatattaac aaatattgct
     5941 tttacattgt tgtcgctaac aatcatttta aaagcttgtg tgacctgttc aggagttgcg
     6001 ccaccaccta catccaagaa atttgccggc tcaccgccat gatacttaat aatgtccatt
     6061 gtagacatag caagtcctgc tccgtttaca aggcatccta tattaccatc aagcttaaca
     6121 tagcttatgc cccactcttt tgcttccagt tctgttgggt cttcttcagt aggatcatgg
     6181 taagcttgta aatctttgtg tctgtaaagc gcattatcat ctatattgag tttagcatcc
     6241 agaggtatca catcatctga ttttgtaaga atcataggat taatttctgc taatgaacag
     6301 tcactgttta caaatacttc atataaccta acaattaaat ttacagcctt attagctgtt
     6361 tttcctttaa gacccagaaa ataagcaatc tttcttccct gaaatggcag taaccctgtt
     6421 gaaatatcaa catattcctt aataattttc tcagggctgt gctttgctac ttcttcaatt
     6481 tctaccccgc cttcagggct tgccattact actatttttt ctttagatct gtctattacc
     6541 attccaagat aaaactcttt ttctatctgc gaggcttctt ctacaagtac cttacctaca
     6601 agctttccct caggccctgt ctgatgggtt acaaggttca taccaataat atctgaggca
     6661 agcctgccta cgtcttcagt agttgaagca agcttcactc caccaccctt tcctcttccc
     6721 ccagcatgta tctgtgcctt tactacaaaa ccatctgact gcagatcatt agcaatctgc
     6781 atggcttctt cgcttgaagt agcaactttt cctgccggaa cgttcattcc gttttttgca
     6841 agtagttctt ttgcctgata ctcatgtatg ttcaactctt tatatcctcc aaaatcattt
     6901 actactcatt aaccttcttt aaatttagaa agtatccagt ttattggatc aaaatattta
     6961 tcaacaactc tttcttttag tgggattatc gcattatcag ttatatgtat actctcagga
     7021 catacctcag tacagcattt tgtaatgtta cagtatgcta caccggcctc ttcttttaat
     7081 agttcgcttc tatcaaccgt atcaagagga tgcatatcaa gttcggcaat ttttatcata
     7141 tatcttggtc caaaaaatgc atccttagcg ttattatctc ttaatatgtg gcaaacattc
     7201 tggcacataa aacattcaat acactttcta aattcctgac ttctttccac atcttcctga
     7261 tacattgtta gttcttcttc accctctgga ggagtgaaag gaggtatctc tttatgtttt
     7321 tgaaaattcc atttcacatc tgtcacaagg tctttaatta cagggaatgc tttaatcgga
     7381 taaacagtga ttggctcatc atgagaaagg ctgtccattc ttgtcttgca tgatagtttg
     7441 ggcataccat taatttcagt actacaagat ccacatttag ccgctttaca attccacctg
     7501 acagctaaat ctccatcaaa attagcctga atgtaatgca gggcgtctaa tacaaccatc
     7561 ccttcttcta caggcacttt gtagtctaca gtctcacctt tttccgaatc gcctctaaat
     7621 actcttagta ccgcttcgtc agccattatg aatcctccga aaataagctt ttaagctcgt
     7681 ctggcatttc tggaagagga gaaagtccaa gattcatttt atctccatcc ttagaaataa
     7741 cagaattcac tttgttccat tttgtattat cgctttcagg gaaatctact ctgctatgag
     7801 cacccctgct ctcttctctt tgtagtgcac attttgctgc agcctctgca acaataaaaa
     7861 gcgaatttaa atccacactc aggttccacc cgggattaaa catcctcgat ccttctacac
     7921 taagaacctg tgagtcatta attaatgctt ctaactcttt taaaccctgt tccaatttat
     7981 ctttaatcct gaatacacca aagtagttct gcattatttc ctgtagattt tgatgaactg
     8041 tgtatgggtt tttgccatta gtattgctaa atggagctag tatgtgtttt tcatgctgtg
     8101 taatttgacc ataattcata tttaaagtat ttgcatctat ttcatttgca tactctgcag
     8161 caccaagccc ggctcttctt ccaaacacca ataagtcaga tagagaattg ccacctagtc
     8221 tgttagctcc atgcattcct gccgctacct cacccgcagc aaatagccca ggcacggtgc
     8281 tttgtccagt ttcaggatca acccttactc cgcccataac atagtgcgtt gtagggccta
     8341 cttccatagg atcttttgtt atatcaatat ctgcaaattc cataaactga tgatacatgc
     8401 tcggaagttt ctttattaca tattcagcgc cacgatgaga tatatctaag tatgccccac
     8461 catgcggagt acctctgcct tcctgagctt cagtataaat tgatcttgct acaacgtctc
     8521 ttgtggatag ctccattctc tcagggtcat atttttccat aaatctttca cccttagagt
     8581 ttgtaagtat tccgccttct cctctaacag cttcagtaac aagtattcct tttacgcttg
     8641 gaggccatac cattccagtg ggatggaact gaataaattc catatcaatt agttctgcgc
     8701 ctgcgttata agcaagtgcc tgcccatcac ctgtatactc ccatgagttg gacgtaacct
     8761 gatatatttt accaataccg ccagttgcaa gtattacagc tttagattta aatagtacaa
     8821 attttccagt ctctctccag taaccaaatg cacccactac cttgtcgcca tctttaacaa
     8881 gatcggtaat cgtgcattcc atataaacgt caatacccga gtgaatacct ttatcctgca
     8941 gcgttcttat taattccagc cctgttctgt cgcctacatg acatagtctt ttccaggtat
     9001 gtccgccaaa agcgcgctga ttaattttac cttctttggt tctatcaaaa agtgctcccc
     9061 attgttctaa ttcatgaatt ctgtcaggcg cttcagtagc atgaatttga gccatgcgcc
     9121 agttgtttag catctttccg cctttcatgg tgtctttgaa atgcgtctgc cagttatcct
     9181 caggggctac atttgccatt gcagcagcaa caccgccttc tgccattaca gtatgagctt
     9241 ttcccaaaag ggatttacat acaagcccaa cagatgctcc ctgtgcagag gcttcaattg
     9301 cggctctaag gcctgctccg cctgctccaa ttacaattac gtcatgttca tgtgtttcgt
     9361 atttatccat ttttaaataa acctcacatc gtttataaca cctgttgcta caagccttac
     9421 atataaatca gtaagacata cgccaaaaag actgagccag gcccagagca tatggttttc
     9481 atttagtgaa cttgctccct tccaaagaga aaatcttgta gggcaggcag aaaatatatt
     9541 gagcttaccg cctattaagt gtctaaatga gtgacaggaa agagaataca tactcaaaag
     9601 aaatgcatta attactaata caaccgatcc tattccaact ccatagccat cttcaaattt
     9661 aaatccttta taggcatcat accaaaggaa aattaatact atcgtagctg cataaaaaaa
     9721 gaatctgtgc aggttttgta atattaacgg gaatttgctc tcgccgttat aactctcttt
     9781 gcttctgcct tcaactgcac accccggggg catccccgca aatgatctgt aatatgcttt
     9841 tctgtaataa taacatgtaa acctgaatag gcccggtgcc catagtatga ggatagcagg
     9901 cgaaagaggc caccactcgg gatttataag tggagaataa aatggtgaaa gatagggttc
     9961 agccagataa tactgatttt cgagcgccct ccatgttgca tatagtacaa aagaaccaag
    10021 tactgcagct gtaatcatag gagctgtctt ccaattatcg gttcttaaag tcgtataatt
    10081 ctgtgatgca gaactaccca ttttttcctc caaactgtaa aatttacaaa acccacttat
    10141 ttttaaatat tcaggctgtt aataaaaaat taaattgtcg ccgaattagg ttttagagat
    10201 gaccaagctc atgtattctg tcacaaagct cttttacatg acctgctgat atatgtaatt
    10261 cttctttttc cttatcagtt aaattgatct caatgatttc ttcaatacct gcccttccca
    10321 gtttaactgg aagacctaca aatgtaccgt caataccata ttcgccttca gcataaacac
    10381 aacaaggcag aattttcttt ttatccttta aaattgcttc tgccatttca acagctgaag
    10441 cacccggtgc atagaatgca ctgccagttt taagcagttt aactatttca gcaccaccat
    10501 ctcttgttct ttgaattatt gattctactc tgtcttccgg cataagttct gttattggaa
    10561 taccggctac agtagtaaat cttgcaagtg gaaccatatc atcaccatgt cctccgagta
    10621 caaatgcctg aatgttatca accgaaacgt ctagctccat tgaaatgaag gttctaaacc
    10681 ttgcagaatc aagcaccccg gccataccta atactctttc ccttggaaag ccactagctt
    10741 tataagccac atatgtcatt gcatcgagcg gatttgttac aagtataagt attgcattag
    10801 gtgatttttt agctacttct gtggttacct gtgttacgat tttagtgttt gtggcaagca
    10861 ggtcatctct gctcattcca ggctttctag gaagtcctga tgtgattatt actatatccg
    10921 aatctgctgt ctcatcataa ccatttgatc ccgtgatgtt tacatcatat ttaacaacag
    10981 ggcctgtctc cattaaatca agcgcctttc cctgcggcat gtcttcaact atatcaacta
    11041 aaactacgtc accaagttcg gattctgcga gtctcaaggc cgctgtagct cctacattac
    11101 ccgctcctat tactgatatt ttaggtctgc tcattatatt ctctccaaag attttaaatt
    11161 aggatttgtt ttatatcatc catattatct gcaatagtct gtccaaactc tgaacatttg
    11221 agaagtgttg ctccttctat ctgtctgtgc agatcatatg taactgtttt atttaaaata
    11281 gttttttcca ttgctttcac aattagctct gcagcttctg tccatccaag atactcaaac
    11341 atcataacac ctgaaagtat caccgaacct ggattaacct tatcctgtcc tgcgtatttt
    11401 ggggctgttc cgtgtgtagc ctcaaacaaa gcgtaaggat caccaatatt agctccaggt
    11461 gctactccca ggccgccaac ctgccctgcc agagcatctg ataagtaatc accgtttaag
    11521 ttaggcatag caagcacgtc gtattcatta cctctggtaa gaatctgctg aaacatatta
    11581 tcagcaatcc tgtcttttat tataatttta tctttaggtg ctttgccatc gtacttatcc
    11641 cacaattcat cctcagtgat tgtttcatta ccgaattctt cttttgcaag ctcataaccc
    11701 caatctctga acgcaccctc agtaaacttc attatgttac ctttatgtac taacgtaaca
    11761 ctctttctat tatatttgat tgcgtactgg attgcttttc ttacaagcct tttagtacca
    11821 aacggactaa taggttttat accaattcca gaaagttcta ttatttctac cttcat
//
LOCUS       DBNGSV010000177         1807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1831877, whole genome shotgun sequence.
ACCESSION   DBNGSV010000177 DBNGSV010000000
VERSION     DBNGSV010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1831877"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC159U_06280"
     CDS             complement(<1..479)
                     /locus_tag="AC159U_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3336701.1"
                     /translation="MNNFFEILDKYKSLQKSALIEESKSISYEELFNLCTSLSSFLCK
                     NQIHKGERVAIFIPNSIEFAVSFFSCLNLGAVAVPINPKFKKYEIEYFINDSEPKLII
                     TSDILKTLLLTVNKELKSRTISISGKNPDFNLHELICSDRILNPNSTSETDKAVYLYS
                     "
     gene            complement(486..1481)
                     /gene="argC"
                     /locus_tag="AC159U_06285"
     CDS             complement(486..1481)
                     /gene="argC"
                     /locus_tag="AC159U_06285"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3336702.1"
                     /translation="MKIKVGILGATGYTGAELIRLLTAHPNTEIKWLTSESFTGQKIC
                     EVFPTFRNIVDLKCKSVREIEELKKVDLVFSCLPKGSSSHFSRLFLDKGAKFIDFSSD
                     LRFNKSPKGTKAIYGLPELFRNKIKKTYLLANPGCYATAASLGLAPLINNKLIKTDSI
                     TIDSKAGLSGGGRAPVVSRQFSEANDAISVDSISDHNQKTEIEELIRNRYKTNSNLIF
                     STYNVNIDRGILTTISAKLDESVTKEKLHSIYMDFYKNERFIRIIENEDAVSTKNIRF
                     TNYCDIGVNIQDRHVVCVTALDNLGKGASGQAVQNMNLMFNLDESAGLLNSGLLP"
     gene            complement(1597..>1807)
                     /locus_tag="AC159U_06290"
     CDS             complement(1597..>1807)
                     /locus_tag="AC159U_06290"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HIR3336703.1"
                     /translation="HILNICGEDHVGIGSDLDGAEIKDFPEDIRAIENLQKIPEYLYE
                     KGYSKDLVRKISYDNFLRVIKSNLR"
BASE COUNT          535 a          315 c          294 g          663 t
ORIGIN      
        1 gagtatagat aaactgcttt atccgtttca ctagttgaat tagggtttaa aatacgatca
       61 gaacatatta gctcatgtaa attaaaatca ggatttttac cactaataga gattgttctg
      121 ctcttcagtt ctttattaac tgttagcaat aatgttttaa gtatatcgga tgtaataatt
      181 agttttggct cggagtcatt aataaagtac tcaatttcat attttttaaa ttttgggttt
      241 ataggaacag caactgcacc caggtttaaa catgagaaaa agcttactgc aaattctatg
      301 gaatttggaa taaagatggc aacccgctca cctttatgaa tttgattttt acataaaaat
      361 gatgagaggc ttgtgcatag gttaaataat tcctcgtagc ttatgctttt agattcttcg
      421 ataagcgcag atttttgtag tgacttatac ttatcaagta tttcaaaaaa attattcatt
      481 tattcctacg gcaataaacc tgaatttagt aaacctgcag attcatcaag attaaacatc
      541 aggttcatat tctgtacagc ctgcccggag gctcctttgc caaggttgtc cagagcagtg
      601 acacatacta catgtctgtc ctgtatattt acaccaatat cacagtaatt agtaaacctg
      661 atgtttttag tactaacagc atcttcattt tctattattc ttataaacct ctcgttttta
      721 taaaaatcca tatatataga atgcaatttt tctttagtaa cagactcatc taatttggct
      781 gatatggtgg ttaaaatacc gcggtctatg ttaacattat aggtcgagaa tattagattt
      841 gagttagttt tgtacctatt tcttataagc tcttctattt ctgttttttg gttatgatct
      901 gatatagagt ctacagagat cgcatcattt gcctctgaaa attgtctgga aacaacaggc
      961 gctctgccgc cgcctgaaag tcctgccttg gaatctatcg ttattgagtc tgtttttatg
     1021 agtttattgt taataagtgg agcaagaccc agagatgctg cagttgcata gcagccgggg
     1081 ttagcaagaa gatacgtttt tttgatctta tttctaaata gttcgggcag gccatatata
     1141 gcttttgtgc cttttgggga tttattaaat cttaagtcag agctaaaatc tataaatttt
     1201 gctcctttat ctaaaaacaa cctggaaaag tggctggacg aaccttttgg aaggcaggaa
     1261 aataccaaat caactttttt aagttcttca atttctctta ctgatttgca ttttagatca
     1321 acaatgtttc taaatgtcgg aaaaacttca catattttct gtcctgtaaa actctcagag
     1381 gttagccatt taatctctgt atttgggtga gcggtaagta atcttattaa ctcagcccct
     1441 gtatatccag tagctccaag aattccaact ttaattttca tgatttgaat ctctttgtat
     1501 tgatcgttat ctaaataggt atagacaatt taataaaata gttattacgt ttccatcctc
     1561 ctccaaaagc ccggagatta tgcttcgggc ttttttttat ctcaggttac tttttataac
     1621 tcttaggaaa ttatcatatg aaattttcct tactagatct ttagaataac ctttttcata
     1681 caaatactcc gggatttttt gcagattttc tatagctctt atatcctcag gaaagtcttt
     1741 tatctctgct ccgtccaggt cgcttcctat acctacatga tcttctccac agatatttaa
     1801 aatatga
//
LOCUS       DBNGSV010000178         8595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1832129, whole genome shotgun sequence.
ACCESSION   DBNGSV010000178 DBNGSV010000000
VERSION     DBNGSV010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1832129"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1365
                     /gene="metK"
                     /locus_tag="AC159U_06295"
     CDS             205..1365
                     /gene="metK"
                     /locus_tag="AC159U_06295"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3336704.1"
                     /translation="MPKGNFIFTSESVTEGHPDKMADQISDSILDAALSGDKHSRVAC
                     ETLITTGLVVLAGEITTNSIIDYPHIVREKIRDIGYTDSNMGFDCDTCAVMVTLDGQS
                     QDIAMGVDENSQKEQGAGDQGLMFGYATDETDVLMPSPIMYAQKLAKRLSQVRKDGTL
                     SFLRSDGKTQVTVRYEDNKPVAIDAVVISSQHSDEVSNKDIYDGIYEEVIKASIDKKL
                     LTKNTKIFVNPTGRFVTGGPMGDAGLTGRKIIIDTYGGWARHGGGAFSGKDPSKVDRS
                     AAYMARYVAKNIVASGIANECEVQLAYAIGVADPVSVMIDTFGTSKVSEDKISEVVRE
                     VFDLKPSGIIKSLNLLRPIYSETAAYGHFGRTGEGFTWEKTDKTEELNTAIR"
     gene            1418..2674
                     /gene="ahcY"
                     /locus_tag="AC159U_06300"
     CDS             1418..2674
                     /gene="ahcY"
                     /locus_tag="AC159U_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3336705.1"
                     /translation="MQHDVKDLALAKEGKLRSEWAFQEMPVLSQIQQRFKKQKPLKGI
                     TIAACLHVTTETGNLAITLKEGGANVALCASNPLSTQDDVAAHLVKNHKISVFAIKGE
                     NTKVYYNHINSALDQKPQITMDDGADLVSTIHTTRKDVLEGVIGGTEETTTGVIRLKS
                     MSKNNILKYPIVAVNDANTKHLFDNRYGTGQSTLDGIIRATNRLISGSTFVVCGYGWC
                     GKGLAMRARGLGANVIVTEIDQLAGLEAVMDGFRVMPIADAAKLGDFFCTVTGNINVI
                     RREHFPNMKDGAIVANSGHFNVELDLDGLKKISKSTRDIREYVKEYTLKNGKRINVLG
                     EGRLINLAAAEGHPSSVMDMSFANQALCAEYMVKNAKKLENIVYDVPPKVDSSVADLK
                     LKALNVKIDKLTPEQDKYLNSWDMGT"
     gene            2688..3575
                     /gene="xerD"
                     /locus_tag="AC159U_06305"
     CDS             2688..3575
                     /gene="xerD"
                     /locus_tag="AC159U_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3336706.1"
                     /translation="MEEYVDSYYSYLAVIKGLSDNTLESYIRDCKKFVLYLKQKNINN
                     LADITHSHILDFLTILKDKNSKPKTIARYIVSIKQFFKYLLIEKIITEDPTVNIKTPK
                     LSVTLPDILTLEDTEVLLKSPDTETTIGQRDSVMLEVLYGSGIRVSELINLKLNSINF
                     EMGCIMVMGKGSKERIVPLGRIAIDKIRSYLQDTREKLLKGKTSDYLFLNRNGKNMTR
                     QGFWKILRQYVIKSGINKVVTPHTIRHTFASHLLERGADLRTIQILLGHSDISSTQIY
                     THIDVKRLKDIHSKHHPRA"
     gene            3579..4259
                     /locus_tag="AC159U_06310"
     CDS             3579..4259
                     /locus_tag="AC159U_06310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3336707.1"
                     /translation="MDINLNFIIIAAPVILFALTIHEYCHALVAFKLGDDTAALQGRL
                     TLNPIMHLDPIGTIMMFLAGFGWARPVPVNPLNFKDPEKGMMLVAIAGPISNIITAIV
                     AGLALKFILSDPSFSVETNGGAYNTLVLILILNVVFGIALAVFNMIPIPPLDGSRVLY
                     AYLPRRFADAYGKLEQYGFFFLIIIFIFAGSLFGKFLWYPISILSKLITGFDYYYLSY
                     LIQFLTNQ"
     gene            4261..5025
                     /locus_tag="AC159U_06315"
     CDS             4261..5025
                     /locus_tag="AC159U_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3336708.1"
                     /translation="METEILESCTVQLESFEGPLDLLLHLIKKHEIDIYDIPIALISE
                     QYNQYIDLMKDLNLDIVGDYLKMAAELGYIKSRMLLPKPQLEEEEEGQDPREELVKRL
                     IEYQRYKDASYQLFSLDLLGRDIYTRKFDELTTMDLQEFQDPIRVDLWTLVDAFKTVL
                     ERKTSSQTEEANLIFEPEIITVEDKMEEIVQRLEEYESFSFFDLFDINGPKSILITTF
                     LALLELIKQQVVAVIQPEQFSDIKITFAGDAQIAEH"
     gene            5012..5578
                     /gene="scpB"
                     /locus_tag="AC159U_06320"
     CDS             5012..5578
                     /gene="scpB"
                     /locus_tag="AC159U_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3336709.1"
                     /translation="MQNIDLDKAIESIIFISERPVSLKDLSKIFTENNKKEIRDALNN
                     LIDYWKESNRGMTLEEVSEGYQFRTRPEYSVFIAKFKDEKPFRLSRAALEVLSIIAYK
                     QPLTKIEVDHIRGVDSSGVFGMLLERGYIKISGRKEVLGRPFIYETTSEFLETFGLKD
                     LKDLPTLKELEEIEESMDKSILSLDLNQ"
     gene            complement(5549..5980)
                     /gene="ybeY"
                     /locus_tag="AC159U_06325"
     CDS             complement(5549..5980)
                     /gene="ybeY"
                     /locus_tag="AC159U_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3336710.1"
                     /translation="MKITILDNNDYFDKDEKKEIKSDVLLILKLLDQSKDCQVCISFI
                     NDSAIRLLNKTYRNINRATDVLSFPQDGPDFSILGDIVISVDTAKRHAERYANSFDDE
                     IKKLLVHGILHLLGFDHKKKKETIVMREKEKELLVKIKAED"
     gene            6036..7367
                     /gene="der"
                     /locus_tag="AC159U_06330"
     CDS             6036..7367
                     /gene="der"
                     /locus_tag="AC159U_06330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3336711.1"
                     /translation="MNTNYKPLVAIVGRPNVGKSTLFNRITGMQKAIVQDMPGVTRDR
                     LYADADWDGREFQVVDTGGLDPDTKDYFLALVKEQIDVALSEADLIVVVLDGKAGLMP
                     QDKEVMELLRKTNKPVLYTINKIDNEKHESSLYEFAELGVDDFFMISASHGRNVDALL
                     DRIVEIIPQKRVEFEKSDHIRVSILGKPNVGKSTLLNKLSGDDRMITSPVPGTTRDSI
                     DCIIKYHKKEYLFVDTAGIRRRSKIDERLEQITVSKAISTIERSDVVLLMIDGQQGPT
                     HQDCRIADLIKSKGKGCIILLNKWDLVPEELAQTPGIQDKLLEQLKGIDYAPVIIISA
                     LNGKNVTKIFSKIDQVYSNLNQKLNTNNLNNYLKELMQATPPPLFKGKETKIYYISQT
                     NVNPPTFVMFTNRTKGFPEHYKSFLENRFREKYDLEGVPIWFIFRSRKDNK"
     gene            complement(7373..>8595)
                     /locus_tag="AC159U_06335"
     CDS             complement(7373..>8595)
                     /locus_tag="AC159U_06335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3336712.1"
                     /translation="KNTKNVLFINHSIRHGGPGKSLFYILKYLDREKIKPFVLIPADD
                     VFSQDLKNEGIYKNIIVDKRFPENVMRPRLGFKWDAGSGANIKFYISILVNIFDLISF
                     VLTSRSILKKNDIDLVYCNGTVAKIVGAFIGLLNWKPVIWHVRNIQLKLPLIFTMNLL
                     SLLPVIKKIICVSIPTAAQFKLSSDKTIVINNGVDIRDFNARKVKAVLREEYKVKKEQ
                     VLIGTTGRIVPRKKYEIFIDIANKLKADRNDLFNKVMFVIVGNTPYYFRIDQLEVLKR
                     QVADSKLSNKFIFTGYKDDVRSYLKDFDIFVLTSDYPDPFPRSVIEAMSFSKPVVGFN
                     IGGITESVLNNENGYLCDPGDLDEMTERIIDLLDSKSKRTKMGNAGLKRIKNLYLAPD
                     RTKDIEDEIIKVLG"
BASE COUNT         3009 a         1480 c         1554 g         2552 t
ORIGIN      
        1 taaatactga tttaagacaa tatcaggata tgcttttttg attgttattc tgattagcag
       61 ttcatatgca attaatctgg tattatatgt gttttccatg gtatatcatt gtatataatg
      121 agacagcctg gttacttgac ttgatgtccc aaaaagatat attagtcggc tttatactaa
      181 catcataatt taaacttatt aactatgcca aaaggaaact ttatatttac atcagaatca
      241 gtaactgaag gtcatccgga taaaatggcc gatcagattt ctgattccat actcgatgcc
      301 gctctaagtg gcgataaaca cagccgtgta gcatgcgaga ccttaataac cacaggcctg
      361 gtagtacttg caggtgaaat cactacaaac tccataattg attatccaca tatagtaagg
      421 gaaaaaataa gagacattgg ttatacagac agtaacatgg gttttgattg tgatacatgt
      481 gctgttatgg tcactctgga cggacaatca caagatattg caatgggagt tgatgaaaac
      541 tcgcaaaaag aacagggtgc aggagatcag gggcttatgt ttggatatgc aactgacgaa
      601 accgatgttc ttatgccctc ccctatcatg tatgctcaga agcttgcaaa aagactatct
      661 caggtaagaa aggacggaac gttatcgttt ctcagatcag atgggaagac tcaggtgaca
      721 gtaagatatg aagataacaa acctgttgct atagatgcag ttgtaatttc ctctcagcat
      781 tcagatgaag tttcaaataa agatatatat gatgggattt atgaagaggt aataaaggca
      841 agtatagata aaaagttatt aaccaagaat actaaaatat ttgtaaaccc aaccggcaga
      901 ttcgtaacag gcggcccaat gggtgatgca ggcctaactg gaagaaaaat tattatagat
      961 acctatggcg gctgggcaag gcacggaggc ggagcatttt ccggtaaaga cccatcaaaa
     1021 gtagacagaa gtgcagctta tatggcaaga tatgtggcaa agaacatagt agcctctgga
     1081 attgcaaatg aatgcgaagt acagcttgcc tatgcaatag gcgtagccga tccggtttca
     1141 gtgatgatag atacatttgg aacctcaaaa gtatccgaag ataagatatc agaggtagtg
     1201 agagaggtat tcgatctaaa accctccgga attattaaat cacttaacct tcttagacca
     1261 atatactcag aaactgcagc atatggtcat tttggtagaa caggtgaagg atttacatgg
     1321 gaaaaaacag acaagaccga agaattaaat acagctataa gataattcaa catctaaaaa
     1381 acaacctaat tacttatata actatttgga ggaaatcatg cagcacgatg ttaaagattt
     1441 agcactagca aaagaaggta aattaagaag cgaatgggct tttcaggaaa tgcctgtact
     1501 ctctcaaatt caacaaagat ttaaaaaaca aaaaccactt aaaggaatta caattgctgc
     1561 atgtctgcat gtaacaactg aaacaggcaa ccttgctatt acactaaaag aaggtggtgc
     1621 aaacgttgct ctatgtgctt ctaatcctct aagtacccag gatgatgtag ctgcgcatct
     1681 tgtaaaaaat cataaaattt cagtatttgc cattaaaggc gagaacacaa aagtatatta
     1741 caaccatatt aatagtgcgc tggatcaaaa acctcaaatt acaatggatg acggcgctga
     1801 tctggtatcg acaatacata caacgagaaa agacgtacta gaaggcgtta taggtggaac
     1861 cgaagaaaca accactggtg ttatccgtct aaaaagcatg tccaagaaca atatactaaa
     1921 atatccgata gttgctgtaa atgacgctaa tacaaaacat ttatttgata acagatacgg
     1981 tacaggacaa agcacacttg acggcataat aagggctaca aacagactta tttcaggttc
     2041 tacatttgtg gtatgcggct acggctggtg cggtaagggt cttgcaatga gagccagagg
     2101 gctaggcgca aatgtgatag tcacagagat tgaccaatta gccggtcttg aagctgttat
     2161 ggatggattt agagttatgc ctatcgcaga tgctgccaaa cttggtgact ttttctgcac
     2221 tgttacagga aacattaatg taataagaag agagcatttt ccaaatatga aagacggagc
     2281 aattgttgca aattcaggtc attttaatgt tgagctcgac cttgatggac ttaagaaaat
     2341 ttccaaatct acaagagaca taagagaata tgtaaaggaa tatacactta aaaatggtaa
     2401 aagaattaat gttctaggtg aaggaagact aattaatctt gcagctgccg aaggtcatcc
     2461 atcaagcgtt atggacatga gctttgcaaa tcaggcctta tgcgccgagt atatggttaa
     2521 aaatgcaaag aaactcgaaa acatagtata cgatgttcct ccaaaggtag atagttcagt
     2581 tgctgatcta aaattaaaag cactaaatgt taaaatagat aaactaacac cagaacagga
     2641 caaatactta aactcctggg atatgggaac ataaatagct cttttatatg gaagaatatg
     2701 tagattctta ttactcttac cttgctgtca ttaaaggttt atcagataat acacttgaat
     2761 catacataag agactgcaaa aagtttgttt tatacctaaa acaaaaaaat atcaataatc
     2821 ttgcagatat aacgcactct catattttgg attttctaac catattaaaa gataaaaact
     2881 ctaagccaaa gactattgca agatatatag tctctattaa gcagtttttc aaatatctgc
     2941 ttatagaaaa gattattact gaagatccta cagtaaatat aaaaacacct aaattaagtg
     3001 taacccttcc ggatatactt acattggaag acaccgaagt tcttttaaaa tcaccggata
     3061 cagagaccac tatagggcag agagactcgg taatgcttga agtattatat ggaagcggaa
     3121 taagggtatc ggagcttatt aatctgaaat taaacagcat taactttgaa atgggctgta
     3181 ttatggtgat gggcaagggt tctaaagaaa ggattgtacc gcttggcaga attgccattg
     3241 ataaaatcag atcatacctt caagatacaa gagaaaaact attaaaaggt aaaacatcag
     3301 actatctgtt tttaaataga aatggaaaaa atatgacccg tcagggtttt tggaaaattc
     3361 ttagacaata tgttataaaa tcaggtataa ataaagtagt aacgccccat actatcagac
     3421 atacttttgc atcacattta ttagagagag gcgctgatct taggactata caaatacttt
     3481 tgggacattc tgatatttca agcacccaga tatacacgca cattgatgtt aagaggttaa
     3541 aagacataca cagcaagcat catccaagag cgtaggatat ggatattaat ctaaatttta
     3601 taataatagc agcacctgta atactgtttg ccctcacaat tcacgagtac tgtcatgcgc
     3661 ttgttgcttt taagcttgga gatgatactg ccgcccttca gggaagactg actttaaatc
     3721 ccataatgca cctggatcca attggaacaa taatgatgtt ccttgcagga tttggttggg
     3781 caagaccagt accagtaaat cctcttaatt tcaaagaccc tgagaaaggc atgatgcttg
     3841 ttgccattgc cggacctatt tctaatataa ttacagccat agttgccgga ctagcactca
     3901 agtttatact ttccgatcca tctttttctg ttgaaacaaa tggcggggca tataacacat
     3961 tagttttaat attaatacta aatgtagtgt ttggaattgc tctagctgtg tttaatatga
     4021 taccaatacc tccactggac ggctccagag ttctgtatgc ttatttaccc cgaaggtttg
     4081 ccgatgctta tggcaaactc gaacaatatg gtttcttttt cttaatcata atttttatat
     4141 tcgcaggtag tctgtttgga aagtttttat ggtatcccat ttcaatactt agtaaattga
     4201 taacaggttt tgattattat taccttagtt acttaataca atttttaacc aatcaataat
     4261 atggaaactg aaatactaga atcctgtact gtacaactgg aatcctttga aggcccttta
     4321 gaccttcttc tgcatttaat aaaaaaacat gaaattgata tttatgatat tccaattgct
     4381 ctaatatccg aacagtacaa tcagtatatc gatttaatga aagacctgaa tctcgatata
     4441 gtgggagatt acttaaagat ggctgctgag cttggttaca taaaatcccg aatgctcctt
     4501 ccaaaacctc agcttgaaga agaggaagaa ggacaagacc caagagaaga actggtaaaa
     4561 agactaatag aatatcagag atataaagac gcttcttatc agttattttc tcttgacctt
     4621 ttaggaagag atatttatac acgcaagttt gatgaattaa caacgatgga cctgcaagaa
     4681 tttcaggatc caataagggt tgacctatgg actcttgtag atgcatttaa aacagtactg
     4741 gaaagaaaga cctcttctca aacagaagaa gctaatttga tatttgaacc tgagataatt
     4801 actgtagaag ataagatgga agaaatagta caaagactcg aagaatatga atcattttct
     4861 ttttttgatt tatttgatat aaacggtcct aaatccatat taattaccac atttttagcc
     4921 cttttagaat taataaaaca acaggtagta gcagtaattc agcccgagca gttttcggat
     4981 attaaaatca cttttgcagg agatgctcaa attgcagaac attgatctgg ataaagcaat
     5041 tgaatctata atatttattt ctgaaagacc tgtaagcctt aaggatcttt ctaaaatctt
     5101 tacagaaaac aataaaaaag agataagaga cgctttaaac aatctcattg attactggaa
     5161 agaaagtaac aggggtatga cccttgaaga agtatcagaa ggctatcaat ttagaacaag
     5221 acctgaatac agcgttttta tagctaagtt taaggatgaa aaacctttta gactaagtag
     5281 agctgcactt gaagttttat ccataatcgc atataaacag cctttaacaa aaatagaagt
     5341 agatcatatc agaggagtag actcatcggg agtattcggg atgcttctgg aaaggggcta
     5401 tataaagata tcaggcagaa aggaagtact cggaagacca tttatctatg aaaccacaag
     5461 tgaattcctg gaaacttttg ggctcaaaga tctaaaagat cttccaacgc taaaagaact
     5521 agaagagata gaagaatcta tggataaatc aatcctcagc cttgatctta accagtagtt
     5581 ctttttcctt ttctctcatc acgatagttt cttttttctt tttatgatca aaacctaaaa
     5641 ggtggagtat gccgtgaact aataattttt ttatctcatc atcaaaactg tttgcatatc
     5701 tctcggcgtg cctttttgcc gtatcaactg aaattacaat atcaccgaga atagaaaaat
     5761 caggaccgtc ctgtgggaaa gatagtacgt ctgtagctct gttaatgttt ctatatgttt
     5821 tattcaacag tcgtatagct gaatcattaa taaatgaaat acaaacctga caatcttttg
     5881 actgatccag taatttaaga attaatagta catcagattt tatctctttc ttttcatctt
     5941 tatcaaagta atcattatta tcaagaattg taattttcat tattttggtt aggtatatta
     6001 acaattaata aatctagatt ctaataatat tatatatgaa tacaaattat aaaccgcttg
     6061 tagcaatagt tggcagaccc aacgtgggta agtctactct ttttaataga ataacaggta
     6121 tgcaaaaagc cattgtgcag gatatgccgg gggttaccag agacagacta tatgccgatg
     6181 ccgactggga cggcagggaa tttcaggttg tagatactgg tggacttgat cccgatacaa
     6241 aagattactt tttggcactg gtaaaagaac aaattgatgt tgcgcttagt gaagctgacc
     6301 tgatagtagt agtattagat ggtaaagcag gtttgatgcc gcaggataaa gaagttatgg
     6361 agcttcttag aaaaaccaat aaacccgtac tatacacaat caataaaata gataacgaaa
     6421 aacatgaatc aagtctatat gaatttgccg agctcggagt tgatgatttt tttatgatat
     6481 cagcttctca cggaagaaat gtagatgcgc tgctagacag aatagtagaa atcattccac
     6541 aaaaaagagt tgaatttgaa aagtccgatc atataagggt ctctatactt ggcaaaccca
     6601 acgttggtaa atctaccctt ctcaacaaac tctccggcga tgaccgaatg attacaagcc
     6661 ctgttccagg tacaacaaga gattcaattg attgtattat caaatatcac aaaaaagaat
     6721 acctgtttgt cgacactgcc ggaataagaa gaagatccaa gatagatgaa agacttgaac
     6781 aaataaccgt atcaaaagcc ataagcacaa tagaaagatc agatgttgta ctgcttatga
     6841 tagatggaca gcagggacct acacatcagg actgcaggat tgccgatctt ataaaatcca
     6901 agggtaaagg ctgtattatt ttactaaaca aatgggacct ggtacccgaa gaactggctc
     6961 agacacctgg tatacaagat aaattactgg aacagttaaa aggtatcgat tatgctcctg
     7021 taataatcat ctccgcactg aatggaaaaa atgtaaccaa gatattctct aagattgatc
     7081 aggtatattc aaatctcaat caaaaactaa acacgaacaa tcttaataat tatctgaaag
     7141 agttaatgca ggctacccct ccccctcttt ttaaaggaaa ggagacaaaa atatattaca
     7201 tatcgcagac aaatgttaac cctcctacat ttgtaatgtt tactaacaga acaaaaggat
     7261 ttcccgaaca ttataaaagc ttccttgaaa acaggttcag agaaaaatat gatttggagg
     7321 gagtacctat ttggtttatt tttagaagta gaaaagacaa taaataatag ttctatccaa
     7381 ggactttaat aatctcatct tctatatctt ttgtcctgtc aggagctaga tacagatttt
     7441 taattctctt tagtcccgca tttcccattt ttgttctctt gctcttacta tcaagaagat
     7501 ctattattct ttctgtcatt tcatccagat caccaggatc gcacagatag ccgttttcgt
     7561 tgttcagtac agactctgta attcctccta tattaaaccc aacaactggt ttagaaaatg
     7621 acatggcttc aatcactgat cttggaaacg gatctgggta atctgaagtt aaaacaaaaa
     7681 tatcaaaatc ttttaaatac gacctcacat catctttgta gcctgtaaaa ataaatttat
     7741 tagataattt gctgtcggca acctgccttt taagtacttc taactgatct atcctgaaat
     7801 aataaggggt attgcctaca ataacgaaca taactttgtt aaacagatca ttgcggtcag
     7861 cttttaattt attggcaata tctataaata tttcatactt ttttcttgga acaattcttc
     7921 cagtcgtacc aatcaatacc tgctcttttt taactttata ttcttctctt agtacagctt
     7981 ttacttttct ggcgttaaaa tcacgaatgt ctactccgtt atttattact attgttttat
     8041 ctgaagaaag tttaaactgt gctgctgttg gtatcgatac acagataatc ttctttataa
     8101 ccggtaaaag tgaaagaagg ttcatagtaa aaataagagg aagcttgagc tgaatatttc
     8161 ttacgtgcca tatgacaggt ttccagttta atagccctat aaatgctcca actatttttg
     8221 ccaccgttcc gttacaatag acaagatcaa tatcattctt tttgagtata gaccgtgatg
     8281 tgagtacaaa agatattaaa tcaaatatat ttactaatat actaatataa aatttaatat
     8341 ttgcccccga gcctgcatcc catttaaatc caagccttgg gcgcatcaca ttttctggaa
     8401 atctcttatc tacaatgata tttttataaa taccttcatt tttaagatcc tgagaaaata
     8461 catcatctgc aggaattaac acaaatggtt ttattttttc tctgtccaga tatttaagta
     8521 tataaaaaag gctctttcct ggacctccat gccttataga gtggtttata aacagtacat
     8581 ttttagtatt cttca
//
LOCUS       DBNGSV010000179         1214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1002429, whole genome shotgun sequence.
ACCESSION   DBNGSV010000179 DBNGSV010000000
VERSION     DBNGSV010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1002429"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1208
                     /locus_tag="AC159U_06340"
     CDS             join(82..331,331..1208)
                     /locus_tag="AC159U_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612266.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3336713.1"
                     /translation="MRKSRFTEQQIAFALKQAETGTKISEVCRKMGVAEATFYNWKKK
                     YSGLGVSELRRLKQLEEENRQLKKLVADLSLDKHMLQDVNSKKALKPALKRSLVKYVD
                     RVYRVGMRRACGVIKMGRSTYYYRSVRDDRAIRKRIIEIAEARVRYGYRRIHVLLRRE
                     GWHVNHKKVYRIYCEDGLNLRRKRPKRMVSAACREMRPVVSKIDQTWSMDFVSDSLFN
                     GRRMRALTIVDNFSRECLAIHADQSIKGDKVVEVMEALKCFMGRVPERIQVDNGSEFI
                     SKALDKWAYENGVVLDFSRPGKPTDNAYIESFNGSFRDECLNTHWFLSLDDAREKIET
                     WRRDYNEYRPHSALDNMTPEEYVKQHIKKPEISNYPGTAFG"
BASE COUNT          405 a          186 c          332 g          291 t
ORIGIN      
        1 tgaccttcac cctgcaaact gaaccaggta aaagtagaga aatctggcaa aataatgcaa
       61 taaatcagga ggtattttgc catgaggaag tcaagattta cagaacaaca gatagcattc
      121 gctttaaagc aggcagagac aggaacaaag atatcggagg tatgcaggaa gatgggggta
      181 gcagaggcta cattctataa ctggaagaaa aaatacagtg gcctgggagt aagtgagtta
      241 aggcgactga agcagcttga ggaggagaac aggcagctta agaaactagt agctgattta
      301 agcctggata agcatatgct tcaggatgta atagcaaaaa agctctgaag ccagccctaa
      361 agaggagctt agtcaaatat gtggataggg tttaccgagt ggggatgaga agagcatgcg
      421 gagtaataaa gatgggaagg agtacttatt actaccgttc tgtaagggat gacagagcta
      481 ttagaaagcg tattattgag attgcagagg caagagtacg gtacggttac agacgcatcc
      541 atgtgttact gaggcgagag ggctggcatg ttaatcataa gaaggtgtac aggatatact
      601 gtgaagacgg gcttaatcta agaaggaagc ggcccaagag aatggtatct gctgcctgcc
      661 gggaaatgag acctgttgta tctaaaatag atcagacatg gtcaatggat tttgtatccg
      721 acagtttgtt taacggcagg cgtatgaggg cattaactat agtggataat tttagtcgtg
      781 agtgtcttgc aatacacgcc gatcaatcta tcaaagggga caaagtagta gaagttatgg
      841 aagcattgaa atgttttatg ggaagggttc ctgagaggat acaagtagat aacggcagtg
      901 agtttatatc caaggctctg gataagtggg catatgagaa tggagtagtg cttgatttct
      961 caagaccggg taaacccact gataatgctt acatcgagtc attcaatgga agttttagag
     1021 atgagtgttt aaacactcat tggtttcttt cacttgatga tgcaagagag aagatagaaa
     1081 cctggaggag agattacaac gagtacagac ctcattcggc acttgataat atgacgccgg
     1141 aggagtatgt taaacaacac ataaaaaagc cggaaatctc taattatccc ggtacagctt
     1201 ttgggtgagg gtca
//
LOCUS       DBNGSV010000180         5955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1139718, whole genome shotgun sequence.
ACCESSION   DBNGSV010000180 DBNGSV010000000
VERSION     DBNGSV010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1139718"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC159U_06345"
     CDS             complement(<1..269)
                     /locus_tag="AC159U_06345"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3336714.1"
                     /translation="MDKHFLEVFFDIQSGLPRQGPGDNDSTRRALELCDLPANANVLD
                     IGCGPGAQTMAVAKELPQATIKAVDFHTPYLKELDQRAQESGFAGR"
     gene            466..879
                     /locus_tag="AC159U_06350"
     CDS             466..879
                     /locus_tag="AC159U_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3336715.1"
                     /translation="MTFGDYLKNQRKNRNITQEDLAKSLGVSSVFIHQLETGKVDAPS
                     VDRCQQLSNIINIPFEQLWNFARKERMRRFMEREGLNEENMEVLCEEEKMLINLYRSL
                     DEDLKKDFSGMIYMLFRHSKDQKIQEILDKFMQCA"
     gene            870..1649
                     /locus_tag="AC159U_06355"
     CDS             870..1649
                     /locus_tag="AC159U_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336716.1"
                     /translation="MRLDLRNNTVLLAVVVELCIFTVVGLVFLFQYKSAKNEFVTKTR
                     AIAETIGDLSGSFFAEGAINPTSDEFYHFLDERLGRKKLFNTFEIAPKFFSVVFKDEL
                     EQQQGGMFIKEVDPVKGYSVFDRDGVISVAVPFSIKDSLSPKGIVKIDSETTAIAKRV
                     LSENFLLYAAMLVVFNNQAFILYLLNRKKKEVVFEKGYLRDNSVGALKIMHKILGDVI
                     TDHELEEKGIQKDDGTSKSTDEKTGDKNVISFSSLSDKKKK"
     gene            1646..2074
                     /locus_tag="AC159U_06360"
     CDS             1646..2074
                     /locus_tag="AC159U_06360"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR3336717.1"
                     /translation="MRYLLTLIFCVFLLAGCIQSENDGFVELDGPILESVNKEGQLEF
                     NGVIVNNGDVAVQSVFVVIILKDESGNVVEANSTPLFDDGNLQALYPYQREFFTISLN
                     SQPDRVFTKEVEIYYDEFNEDEVLDVDKSDEELLDTEFVN"
     gene            complement(2071..3033)
                     /locus_tag="AC159U_06365"
     CDS             complement(2071..3033)
                     /locus_tag="AC159U_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3336718.1"
                     /translation="MLTFSISPLLSQEYSNSFESDYKKAKTHIQASLNFLEQSNKAKL
                     SQNEQSSQLYFHLSQIHLSTAQSLFRTSTIKSQTELLKEELASIQSKNFKIKEQFKDN
                     ISKLKEHKDKITLSEELLYNNSLNNLETALTQINAAEDVDATVYSSHLLTQAHNYYQK
                     ASENHEQGKYELSATESDKSIELAKRAFDESSNKFQAKQGLKDKFANIFGFSINSSDS
                     SLVLLSTELFSPQSSSIRFDLYPSLDKIAGIIKNSDDIKLEIKAHNNSYKSRTKNKKL
                     SAEQRDTIMTYLISKGITENAFIINDYPSKEITAERKVEIILIN"
     gene            complement(3072..3494)
                     /locus_tag="AC159U_06370"
     CDS             complement(3072..3494)
                     /locus_tag="AC159U_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336719.1"
                     /translation="MSILFQRKLEFTLIFTLLLFSSFTVNSVIYAQKIDNADKVKELS
                     ELISNLEDKQHPISQDSLELSKDFVININNGSYNSADPSIIDELFKAHSALLNALLVE
                     ARNSTELQSTKNTIAEQQQLYEKLSKYNTDISQDLKKY"
     gene            3799..4848
                     /gene="pilM"
                     /locus_tag="AC159U_06375"
     CDS             3799..4848
                     /gene="pilM"
                     /locus_tag="AC159U_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR3336720.1"
                     /translation="MFFSKKGVVGLDIGSSSIKLVELKGSKKGFQLKNLGEALLPPDA
                     IVNKVINNSENVIDSIATLVDDLNIKSKNVAIGVSGHSVIIKKVSMPKMTDKELADSI
                     QWELEQYIPQSVEDVNYDFQILPGETAEGNMEVLIIAAKKDIAAGYLNAVTDAGLNPV
                     IVDVDVFALENMYDENYFESQGVVALVNVGASIINVNILREGISIFTRDITTGGNQFT
                     EMIQKEFDVNFEEAEKMKYSLGSDSSSQELKRIAGDFNNMICGEIKRTLDFFTNTIWK
                     EKVNSVLIGGGSSKIPEMEETLKSMANTNVELLNPFRNIGYSNKDFDTDYISDIGPKM
                     SIATGLALRKVGDRP"
     gene            4845..5678
                     /locus_tag="AC159U_06380"
     CDS             4845..5678
                     /locus_tag="AC159U_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3336721.1"
                     /translation="MIKINLLPVEERRSVEGFGQFVFGILIIAVAIALMVGAIIVQNN
                     KIKDIREETARVNKRIKELDSIRKQVEKFKVQNKKLEQRIKVIAQLEKNRVGPLYVMD
                     SLSTEIPERAWIGSFTTRGDSAEISGIASSEFVISEFMRNLESSPHFRNINLSKITNK
                     KVSGKTFKSFGLSARVKFFKPPEETEAKVSDETADSTVMKSVSDTTVLNTAEKSVMTD
                     GMEMIDKSKSQIKEENKSDKDLKDTNTDVDNNNDKKPETKKKPTTGVVGSDDGSNVIV
                     F"
     gene            5703..>5955
                     /gene="gspM"
                     /locus_tag="AC159U_06385"
     CDS             5703..>5955
                     /gene="gspM"
                     /locus_tag="AC159U_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR3336722.1"
                     /translation="MIEDLVESINQKSVQSRILILLAVLAVLGVIFYYVFWEPKQTEI
                     KGLQTQLQKREKLKNEYENIAKELPKFKAENKRLEKEFLQ"
BASE COUNT         2051 a          831 c         1157 g         1916 t
ORIGIN      
        1 cgaccggcaa atccagactc ttgtgcccgc tggtccagct cttttaaata tggagtatga
       61 aaatccacag cttttatagt tgcttgcggt aactcttttg caactgccat tgtctgtgcg
      121 ccgggaccgc atcctatatc aagaacgttt gcattagcgg gcaaatcaca aagttctaat
      181 gctctgcgcg tactatcatt atctcccggt ccctgccttg gtaaaccgga ttgtatatca
      241 aaaaatactt ctaaaaaatg cttgtccatt ttatcttcta ttttgtgtta aatttgcgat
      301 ataaagcatt tgtatattat gatttatgag aaatttacta tctttgtgta gatttaagta
      361 aaattcttaa aatttctata taaatccatt ttttaatgat ataatataaa tcaatatagg
      421 attgttgtat ttatacaatt ttaaaaactt agttggaggc catatatgac gtttggcgat
      481 tatcttaaga atcagagaaa aaatagaaat attacccagg aagaccttgc aaagtctctt
      541 ggggtatcaa gcgtatttat acatcaattg gaaacaggca aagtagacgc gccttctgtg
      601 gacaggtgcc agcagctttc caatatcatt aacattccct ttgagcagct ttggaacttt
      661 gcaagaaagg aaagaatgag acgctttatg gaaagagagg ggcttaatga agagaacatg
      721 gaagttctct gtgaagaaga gaaaatgctc atcaatcttt acagaagtct ggatgaggat
      781 ttaaaaaagg attttagcgg aatgatatac atgctattta gacattccaa ggatcagaaa
      841 atacaggaaa tattagataa attcatgcaa tgcgcctaga tttaagaaat aacacggtac
      901 ttttagcggt agtggttgaa ctgtgtattt ttacagttgt aggattggtg tttctttttc
      961 agtataaaag cgccaagaat gaatttgtta ccaaaaccag agctattgca gaaacaatag
     1021 gtgatctttc gggaagcttt tttgccgaag gggccataaa tcctacaagt gatgagttct
     1081 accatttttt agacgagagg cttggaagga agaagctatt taatacattt gaaatagcgc
     1141 caaagttttt tagcgtagta tttaaagacg aactggaaca gcagcagggt ggaatgttca
     1201 taaaagaagt agatcccgta aagggctatt ctgtttttga tagagacgga gtaatttctg
     1261 ttgccgtacc gttttctata aaagatagtt taagtccaaa gggaatagtt aaaatagatt
     1321 ctgaaaccac ggcaatagca aaaagggttt tatctgaaaa cttccttctc tatgcagcaa
     1381 tgctggttgt ctttaataac caggccttca ttttgtatct gctaaacaga aagaaaaaag
     1441 aagtggtatt cgaaaaaggg tatctgaggg ataattcagt aggagccctt aagataatgc
     1501 ataagattct tggggatgtg atcacagacc acgaactgga agaaaaaggc atccaaaaag
     1561 atgatggaac atcaaaaagt actgatgaga agactgggga taaaaacgta atatcatttt
     1621 cttcattatc ggataagaaa aagaaatgag atacttactt acattaattt tctgtgtttt
     1681 cttgcttgcc ggatgtattc agtccgaaaa tgacggcttt gtagagcttg atggtcctat
     1741 actggagagc gtaaacaagg aagggcagct tgagttcaac ggagttatcg taaataacgg
     1801 agatgttgcc gtgcagtcag tgtttgtcgt aataatctta aaagatgagt cgggaaatgt
     1861 agtagaagcc aattccaccc ctctttttga tgatggaaat ttgcaggcac tttatccata
     1921 tcaaagagaa ttttttacca taagtttaaa ttcgcaacct gatagggttt ttacaaaaga
     1981 agtagagata tattatgacg aatttaatga agatgaagtt ttagatgttg ataaatccga
     2041 tgaagagttg ttggatacag aatttgtaaa ttagtttata agaattattt ctacttttct
     2101 ctctgctgta atttctttgc ttggataatc attaattata aaagcgttct ccgtaattcc
     2161 tttagatatt aaataagtca taatcgtatc tctttgttcg gcagagagtt tcttgttttt
     2221 tgttctactc ttgtaactgt tattatgagc cttaatttcc agttttatat catcactgtt
     2281 tttaataatg ccggcaattt tgtccaacga agggtataaa tcgaacctta ttgaactgct
     2341 ttggggagaa aacagctctg tagataataa aactagtgaa ctgtctgaac tatttatgct
     2401 aaaaccgaaa atatttgcaa atttgtcctt gaggccttgt tttgcctgaa atttgtttga
     2461 agactcatca aaagcccgtt ttgccagctc aatactctta tcagactctg ttgcggagag
     2521 ctcgtatttt ccctgttcat ggttttctga agctttttga taatagttgt gagcttgagt
     2581 taacagatgt gaactataaa ccgtagcatc aacatcttca gctgcattaa tctgtgtaag
     2641 ggctgtttct aaattgttta gagaattatt gtacaaaagt tcttcggata atgtgatttt
     2701 atctttatgt tcttttagct tcgatatatt atccttaaat tgttctttaa tcttgaaatt
     2761 ctttgattgt attgaagcaa gctcttcttt tagcagttct gtttgcgatt ttatcgtact
     2821 ggttctaaat aatgattgag cagtgctaag gtgaatttgc gataaatgaa aataaagctg
     2881 cgagctttgc tcgttttggg ataatttggc tttattggac tgttctaaga agtttagaga
     2941 tgcttgtatg tgtgttttag cttttttata atctgattca aatgaattgg aatattcctg
     3001 tgataataag ggtgatatgc taaaagttaa catataacac atcaaagcta ttacaatttt
     3061 tgttttatgc atcagtattt ttttaggtct tgagatatat ctgtattata tttggataac
     3121 ttttcataaa gctgctgctg ttctgcaata gtattctttg tgctctgcag ctctgtacta
     3181 tttcgggcct caaccaatag cgcatttaac aaagctgaat gagctttaaa taattcgtct
     3241 attattgacg gatcagcaga gttataagac ccattattta tgtttattac aaaatcttta
     3301 gatagctcta gtgagtcttg cgatattgga tgttgtttat cttctaaatt gctaattagt
     3361 tcagaaagct ctttaacttt atcggcatta tcaatttttt gagcgtatat taccgaattt
     3421 acagtaaaac ttgaaaataa taataacgta aaaattaaag taaattctag ttttctttga
     3481 aaaagtattg acatctgatg agttagaaag tattgttaaa gtaaatgttt aaatatttaa
     3541 atgtgtatat tgttaattta agaattaata ttaaagataa taattatatt agtcaatatt
     3601 tattatgata aaaagtttaa ctaattaaat ctaaagatga tgaaagtaat aaaaaacatt
     3661 aaattattaa agttaaacat tagttatatg attcaactaa ttgatattaa atatatattt
     3721 ttgatgcaat ttcaagtaat gggtattgat aaatttgaaa aaatatgttt taattctatt
     3781 aatcatcagg gaggatgaat gtttttttca aaaaaaggcg tagttggact cgatattggt
     3841 tctagttcaa taaaactcgt agaacttaaa ggtagtaaaa aaggttttca gttaaaaaac
     3901 cttggagaag cactactccc tccagatgca attgttaata aggttataaa caattctgaa
     3961 aacgtcatag actccatcgc aactctggtg gatgacttaa acattaaatc caagaatgtt
     4021 gccataggag tttcaggtca ttcggtgatt attaaaaaag taagcatgcc aaaaatgact
     4081 gataaggagc ttgctgattc aattcaatgg gaattagagc agtatatccc gcagagtgtt
     4141 gaagatgtta actatgattt tcagatactt ccgggagaaa ctgctgaagg caacatggaa
     4201 gttcttatta ttgccgcaaa aaaagacatt gcagccggtt atttgaacgc cgtgaccgat
     4261 gcgggcttaa atccggtaat agtggatgta gacgtttttg ctttagagaa tatgtatgat
     4321 gagaattact ttgaaagtca gggcgtagta gctttagtaa acgttggagc ttcaattatt
     4381 aacgttaaca ttttaagaga aggaatatct atatttacta gagatattac aaccggcgga
     4441 aatcaattta ctgaaatgat acaaaaagaa tttgatgtaa attttgaaga agctgaaaaa
     4501 atgaaatatt cactgggttc tgatagctct tcgcaagagt taaagagaat tgccggtgat
     4561 ttcaataata tgatatgtgg tgaaattaaa agaactctgg actttttcac taatactatt
     4621 tggaaagaaa aagtaaattc tgtactcatt ggaggcggat ccagcaaaat tccagaaatg
     4681 gaagaaactt taaagtctat ggcaaatact aatgtggagt tattaaatcc ttttagaaat
     4741 ataggataca gcaacaagga ttttgatact gattatatta gtgatatcgg tcctaaaatg
     4801 agtattgcta ccgggcttgc acttagaaag gttggagaca ggccatgata aaaattaatc
     4861 tattaccagt agaagaaagg cgatcagtcg aaggcttcgg tcaatttgtc tttggcattc
     4921 ttataatagc tgttgctata gctcttatgg taggggccat tattgttcaa aataataaaa
     4981 taaaagatat cagggaagaa actgcaaggg taaataagag aattaaagaa cttgatagta
     5041 tcagaaagca agtcgaaaaa ttcaaagttc agaataagaa gcttgaacaa agaattaaag
     5101 tcatagctca gttggagaaa aatcgtgttg ggccactgta tgtaatggat tcactatcta
     5161 ctgagattcc tgaaagagcc tggatcggtt catttactac aagaggagat agtgcagaaa
     5221 tttcgggtat agcaagttct gaatttgtaa tatctgaatt tatgagaaac ctggaatcat
     5281 cacctcattt tagaaatata aacctttcca agataacaaa caagaaagta agcggtaaaa
     5341 catttaaaag ttttggacta agtgcgagag ttaaattttt taaaccaccc gaggaaactg
     5401 aggctaaagt atcagacgaa actgccgatt ctactgtcat gaaatcagta tcggatacaa
     5461 ctgtactaaa cacagcagaa aaaagcgtta tgacagacgg aatggaaatg atagataaat
     5521 ctaaatcgca aataaaagaa gaaaataaaa gtgataaaga tttaaaagac actaatactg
     5581 atgttgataa caataatgat aaaaaaccgg agacaaaaaa gaaacctaca accggtgtag
     5641 taggttctga tgatggctct aatgtgattg tattttaata gaaaaaagag gcttatatgt
     5701 caatgataga agatctagtt gaaagtatta atcaaaagtc tgtacaatcc agaatattaa
     5761 ttcttttagc agtactggca gttctgggtg tcatttttta ttacgtgttc tgggaaccaa
     5821 aacagacaga aatcaaaggt ctgcagactc aattacaaaa gagagaaaaa ctaaaaaatg
     5881 aatacgagaa tattgcaaaa gaattaccaa agtttaaagc tgaaaataaa aggttagaaa
     5941 aagaattttt acagg
//
LOCUS       DBNGSV010000181         3553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_32379, whole genome shotgun sequence.
ACCESSION   DBNGSV010000181 DBNGSV010000000
VERSION     DBNGSV010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_32379"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1321)
                     /locus_tag="AC159U_06390"
     CDS             complement(182..1321)
                     /locus_tag="AC159U_06390"
                     /inference="COORDINATES: protein motif:HMM:NF027594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HIR3336723.1"
                     /translation="MSLNKIALRLRESEKQIVNEILCANPYNIEFITNKIEQLSESFS
                     LDSILEILFRQQANCGFVLSQPDSDELIESKYILDSLTGIKFKVQWNPNRELRLNHEL
                     LIERGVININIDNNLLVNRNNQGKACYLCSENIKLQNPLEILLPFKLSGEKYFCGANF
                     SPITNKHFTIMSEEHKPQQYDSQVILAMIDFVTQSNGKFRTIFNGKAGASILEHLHIQ
                     ATTELFPIEDINISNNNLVSTISNTNIYKPDYYLPLLILESENTETISIISDKLINQW
                     ETQNSEYHTVNIVAHKNNEKYRIYIYLRDTRKLKGTGKIGDMGTFECSGLLVLSAGDK
                     DSKKGITGERCLYEQADLGKIKKLLQEIAPTDHFDLTNFISENHK"
     gene            complement(1440..2048)
                     /locus_tag="AC159U_06395"
     CDS             complement(1440..2048)
                     /locus_tag="AC159U_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336724.1"
                     /translation="MRNILFASLLCFSFLVVSCEKTQEVVEDAKETTEKVVEDTKEMA
                     GDAADAVKDTADEAIEAAGDAVDAAGDAAADAADAAKDTAGEAMDAAGEAVDAAGDAA
                     ADAADAAKDTAGEAMDAAGEAADAAADTAGEMKKEAGEMVDNAGDLADDAVDAAGDAA
                     MDAAKDAAGDAAMDAAKDAAGDAAMDAAKDAAGDAAKDAAGY"
     gene            complement(2122..3453)
                     /locus_tag="AC159U_06400"
     CDS             complement(2122..3453)
                     /locus_tag="AC159U_06400"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3336725.1"
                     /translation="MTEDSNNQHRVVIIGGGFGGLYAAKELGDTNIKLTLVDRRNFHL
                     FQPLLYQVATGGLSPGDIASPLRAVLNKQNNTKVYKSEVIDIDVEAKKVVLRYRTIPY
                     DTLIVASGSSHHYFGNDNWAIYAPGLKTIEDALEFRKRIFFAFECAEKITDPKEQESW
                     MTFVVIGGGPTGVELAGALGELANTTLSKDFRNIDTTKTKIILLEATDKILQTYPEKL
                     SNDAVKSLQKLGVTVQTGSFVTAIDENKVTLKKSDRSQIINSKTVLWAAGVRASRLGR
                     VLHKRTGVEIDNIGRVMVNNDLTIDNHPEIFVIGDLANFSHQTGHALPGVAPVAMQQG
                     MYTARLIKKRLNSEQFKPFIYTDKGSLAVIGRNSAVASIGKFKVKGFIAWLIWLFIHI
                     RYLVEYDNKILVLTQWAWNYFTRKRGARLVTGTDHLEILKKEEQEINNNLK"
BASE COUNT          956 a          725 c          562 g         1310 t
ORIGIN      
        1 tattttatgc agagataccg tttgcctacc tcctttactt ttgggtacta tgtgatgttt
       61 atcaaatggt gccaccagat ttctaccata aagtgaacaa attgtgcatt gttcagccat
      121 agtattatat taattctata tattaatttt ttaatataga cataatattg tcactttact
      181 attacttatg gttttcagaa ataaaattag ttaagtcaaa atgatcagtt ggagctattt
      241 cttgtaacaa ctttttaatc ttgccaaggt ctgcttgctc atataaacac ctttctcctg
      301 ttattccctt tttggaatct ttatctccag cagataagac cagcaaacca ctacactcaa
      361 atgttcccat atcaccaatt tttcctgtac cttttaattt tcttgtgtct cttaaataaa
      421 tatagattct atacttctca ttgtttttgt gtgcgactat gtttactgta tgatattcag
      481 aattttgtgt ttcccactgg ttaatcagtt tatcagatat tattgaaatt gtttcagtgt
      541 tttcactctc taatattaat agtggtagat aataatcagg tttatatata ttcgtattag
      601 atatagttga tacgagatta ttatttgaga tattgatatc ttctattgga aataattctg
      661 tcgttgcctg aatgtgtaaa tgttcaagaa tagatgctcc ggcttttcca ttaaagattg
      721 tcctgaattt tccgtttgat tgtgttacaa aatctatcat ggctaaaatc acttgtgagt
      781 cgtattgctg tggtttatgt tcttcagaca taatcgtgaa atgtttattc gtaataggtg
      841 aaaaattggc tccacaaaaa tacttttcac ctgaaagttt gaacggcagt aatatttcaa
      901 gaggattttg aagttttata ttttcactac aaagatagca tgcctttcct tgattattcc
      961 tattaactaa aaggttgtta tctatattta tattaataac ccctctttca atcaatagtt
     1021 catgattgag ccttaattct ctattaggat tccattgtac tttaaattta atacctgtca
     1081 gtgaatcaag aatgtacttt gattctatca attcatctga atcaggctgt gagagtacaa
     1141 aaccacaatt tgcctgttgc ctgaataata tttctagtat tgaatctaaa ctaaaagatt
     1201 cactaagttg ttcaatctta tttgtaataa actctatatt gtaaggattt gcacaaagta
     1261 tttcatttac aatttgcttt tcactttctc ttagtcttag agcaatttta ttcaacgaca
     1321 tagtatagtt tgttgtattc tgataaaaaa ataggggaac atcttagcta gatgttcccc
     1381 tacatgagtt tttaaaatat tttaatagaa gtattactta ttttttttaa ctggatattt
     1441 taatagcccg cagcgtcttt tgcagcgtct ccggctgcgt ctttagctgc gtccattgct
     1501 gcgtctccgg cggcgtcttt agctgcgtcc attgctgcgt ctccggcagc gtctttagct
     1561 gcgtccattg ctgcatctcc tgcagcgtct actgcatcat ctgctagatc accagcatta
     1621 tcaaccattt ctccggcttc ttttttcatt tctcctgcag tatcggctgc ggcgtcagct
     1681 gcttccccgg cggcgtccat ggcttctcct gctgtatctt ttgctgcgtc tgcggcatct
     1741 gctgcggcgt ctccggcggc atcaactgct tcacctgctg catccattgc ttccccagct
     1801 gtgtcttttg ctgcgtctgc ggcatctgct gcggcgtctc cggcggcatc aaccgcatca
     1861 cccgctgctt caatggcttc atcagctgta tcttttactg cgtctgcggc atcacctgcc
     1921 atctcctttg tatcttctac tactttttct gttgtttctt tggcatcttc tactacctct
     1981 tgtgtttttt cacacgaaac aactagaaaa ctaaaacata gtaaacttgc gaataagatg
     2041 tttctcatct tttcctccta aacttaaaaa ttttacttaa ttttgaacaa taaatcagat
     2101 aaaatcaaat atctggatca tttattttaa attattattt atttcctgtt cttccttttt
     2161 taatatttcc agatgatctg taccagtcac cagtcgtgct cctctttttc ttgtaaaata
     2221 attccaggcc cactgtgtaa gaaccagtat tttattatca tattcaacca aatatctaat
     2281 atgtataaaa agccagataa gccaggcaat gaaccctttt actttaaatt tacctatact
     2341 tgcaacagca ctattcctac cgattacagc aaggcttccc ttgtcagtat atataaacgg
     2401 tttaaattgt tcagagttca atcttttttt aattaacctg gcggtataca tcccctgctg
     2461 cattgctaca ggagccactc ccggaagagc atggcctgtt tgatgtgaaa agtttgccaa
     2521 atcacctatg acaaatattt caggatggtt atcaatggtt aaatcattat taaccattac
     2581 cctgccaata ttatcaatct ctacccctgt tcttttatgt aaaacccttc caagtctgga
     2641 cgctctaacc ccagctgccc aaagtactgt tttagaattt attatttgag acctatcgga
     2701 tttttttagt gttactttat tttcatcaat tgctgttaca aaggagcctg tttgaacagt
     2761 tactcccaat ttttgtagag atttaacagc atcatttgat aacttttcag gatatgtctg
     2821 taggatctta tccgtagcct cgagaagaat tatttttgtt tttgttgtgt ctatattcct
     2881 gaagtcctta ctcagagtag tattagcaag ctcccctaga gcaccggcaa gctcaacacc
     2941 cgtgggccct cctccaatca ccacaaatgt catccatgat tcctgctctt taggatcagt
     3001 tattttttcg gcgcactcaa aagcaaagaa aatacgtttt ctaaactcta gggcatcttc
     3061 aattgtcttt aatccaggtg cataaatagc ccagttatca tttccaaaat aatgatggct
     3121 tgaacctgaa gcgactatta aagtgtcata aggaattgtc ctgtatctca gtacaacttt
     3181 ttttgcctca acgtctatat cgataacctc tgatttatat accttagtat tattttgttt
     3241 atttagtact gctctaagtg gagaggcaat atctcctggg gacaaccctc ctgtagctac
     3301 ctgataaaga agtggctgga ataaatgaaa atttctccta tcaaccaggg ttaatttaat
     3361 attagtatcg ccaagttctt tggctgcata tagtccgcca aagccgccac ctattataac
     3421 tacacggtgt tgattgttag agtcttcagt catttatgaa tttataatgg aatggaatct
     3481 gaaaaatctt aaggacagag tcgttcgata atccatttat cttctttttg taatctatat
     3541 ctaagtctgt cat
//
LOCUS       DBNGSV010000182         3994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1764421, whole genome shotgun sequence.
ACCESSION   DBNGSV010000182 DBNGSV010000000
VERSION     DBNGSV010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1764421"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC159U_06405"
     CDS             complement(<1..752)
                     /locus_tag="AC159U_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3336726.1"
                     /translation="MKNRTGKVLYVGKAKHLKKRVLSYFTPNRSDNRYQIHDLLKEVK
                     NLEYIITQNEREALILENSLIKKYRPRFNVQFKDDKTYFSLKLDIKDKYPRLYYTRKV
                     VDDGALYFGPFTSSTTLKKTKKFFHKLFPLRDCTNSKFKRHSDRPCINYNLKLCSGPC
                     AGKISESEYQSLSNQARLYLRGKFNEIVKLIQNNMKQAAEDMRFEEAVLYRNQLSFLK
                     MHIDQKGLIDSNTKDIDVLGIYNEGRGASIVVL"
     gene            complement(962..1219)
                     /locus_tag="AC159U_06410"
     CDS             complement(962..1219)
                     /locus_tag="AC159U_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336727.1"
                     /translation="MRYVVIVLLAALLFTTPSFARPKQITEYDPNDAINPFKITSLII
                     RPPIALINVFVKGFYYVLDSDPIRRPFNIEFDSSINIDEDY"
     gene            1367..2134
                     /locus_tag="AC159U_06415"
     CDS             1367..2134
                     /locus_tag="AC159U_06415"
                     /inference="COORDINATES: protein motif:HMM:NF053169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8083 family protein"
                     /protein_id="HIR3336728.1"
                     /translation="MATSNLIIYEPLDSFIEQKDYWESYIEFYELYWGGPPSPFGVWF
                     ANEYDNFKKSFSERIDINPSEVKDCFFIKDEDGKYYIAPSESETQNNLIYIENIIPVE
                     WFLLFIEDERKSLFSHWGFNAIYYSIKLRSGIERVKTAKNLYEQTISKVPEDIKESEI
                     ISILTNLIRGCDQLLGLLSGYDSSGHIILNYGDICTFIHAYTLDNEKSVGDLSAFNEL
                     IRKSEYEKAATSIKILFQKWNEIFENASGQSSSKPIQ"
     gene            2263..3411
                     /locus_tag="AC159U_06420"
     CDS             2263..3411
                     /locus_tag="AC159U_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3336729.1"
                     /translation="MYDENNFSTVTEEKQYNEFELNRENSFKINKANQKLNTKTSGLT
                     PQYNEKDNILINDYFKDVSLETLLKPKQELTLSALMQNCENRISFYSSPISDVKINKV
                     CECSSSERKIKSVKQKKELNKRAVRNKHIIHAYSKFKNNLRNRFINSNLRLVASIAKR
                     FVGKGASFLDLIQEGNIGLIRAVEKFDYTKGYRFSTYAVWWINQSISRAIFNQTRTVK
                     VPSYLLEKSGKIWQTFIKLKEEKGKDPSAEEISAILNISVDGIKSVIRTGNDVISLDS
                     TIYNSETTSYVDLLKDDNQVFQDAEIDNISIPENVNEALVDLTTREREVIKMRYGIGY
                     DDSFTLDYIGQKFGLTRERIRQIEKQTIMKLKKSHSSEVLKSLYDNLQ"
     gene            3508..>3994
                     /locus_tag="AC159U_06425"
     CDS             3508..>3994
                     /locus_tag="AC159U_06425"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3336730.1"
                     /translation="MCSYTKIKSSLGDVYAVSSSGVLSNLILGLENFEEFRANNKSAE
                     FTRSDEPSAVLKELKSYLDGDLKKFSYKLDISKGTKFQKSVWKELLKIPFGKLITYKD
                     IANKLGRPNSYRAVGNAFGANPLPIVIPCHRVVSKTGLGGYSCGINYKKKLLEHEGIN
                     YQ"
BASE COUNT         1384 a          597 c          738 g         1275 t
ORIGIN      
        1 agaacaacaa tgctcgcccc cctgccttca ttgtatatac caagaacatc tatatctttt
       61 gtgtttgaat caataagccc cttttggtct atatgcatct taagaaaact aagctgattc
      121 ctgtataata cggcttcttc aaagcgcata tcctctgctg cctgtttcat gttattttgt
      181 atcagtttta ctatttcatt gaacttacct ctcaaataga gccttgcctg atttgaaagg
      241 gactggtatt cactctctga gatctttcct gcacatgggc ccgagcacag cttgaggttg
      301 taatttatac acggtctatc ggagtgtctt ttaaatttag agttagtaca gtctcttagg
      361 ggaaaaagct tatgaaaaaa cttcttggtt tttttaagcg ttgtcgatga tgtaaacggt
      421 ccgaaatata gtgcaccgtc atctactacc tttcttgtat aataaagcct gggatattta
      481 tccttgatat ccagttttaa agaaaagtag gttttatcgt ctttaaactg tacattaaat
      541 cttggcctgt atttttttat aagtgaattt tcgagtatca gcgcctctct ttcattctgc
      601 gtgattatat actctaggtt cttaacttct tttagaagat catgaatctg atacctgtta
      661 tcacttctat ttggtgtaaa ataggataaa acgcgctttt ttagatgctt tgcctttcct
      721 acgtaaagta ccttgccagt ccggtttttc atcaaatata caccgggtgc agtgggggca
      781 ttatcgatta tatcattgct atggatcatt aatttaattt ggttaaagct atattttatt
      841 taaaattata gaaaataagt tgagaaatac aaagaagtga ttaaataaga ggtaaaacgg
      901 gcaaagaaaa acccattgca gatagatgca taattatcta caatgggtct tatagtagtt
      961 tttaatagtc ttcgtctata ttaattgaag aatcgaattc aatgttaaaa ggtcttctga
     1021 ttggatcaga atctaaaaca tagtagaaac cctttacgaa aacattaatt agtgctatag
     1081 gtgggcgtat gatcaaactt gtaattttga aaggattaat ggcatcatta ggatcgtatt
     1141 ctgtgatctg tttaggtctt gcaaaagacg gtgtagtaaa cagtaaagct gccagtaaaa
     1201 cgataactac atatctcatg gatatatcct cctgtttaaa attaatatcc agtataaact
     1261 gtttttttct taaggtcaat aataaaccta agtttttcaa gcagtttaat actataatta
     1321 atgtaaattg ttaaagttat tttatcatat tttataaaat attactatgg ctacaagtaa
     1381 tttaataata tatgagccgc tcgattcatt tatcgagcaa aaagattatt gggagagtta
     1441 tatagagttt tatgagctat actggggcgg tcctccatcg ccttttggcg tatggtttgc
     1501 taatgagtat gataatttca aaaaatcttt tagtgagaga atagatatta acccgtctga
     1561 agttaaggac tgctttttca ttaaagatga agatggaaag tattatattg cgccgtctga
     1621 gtcagaaact caaaataatc ttatatatat agaaaacatc attcccgtag aatggttttt
     1681 actgtttata gaggatgaga gaaaatcttt attttctcac tggggtttta atgcgattta
     1741 ttatagtata aagcttagaa gtggcataga aagggtaaaa acagcaaaga atttatatga
     1801 acaaaccata agtaaagtgc cggaggatat caaagagtct gaaatcatta gtattcttac
     1861 aaaccttata agaggctgcg accagctgtt aggactgctt tcaggttatg atagttcggg
     1921 ccatataatt ttaaattatg gagatatttg tacatttata catgcttata ctctcgataa
     1981 tgagaaaagt gtgggagatt tatctgcatt taatgaactt atcagaaaat cagagtatga
     2041 aaaagcagct acttctataa aaatactgtt tcagaaatgg aacgaaatct tcgaaaacgc
     2101 ttccggacag agctccagca aacctattca ataagtaatt tacttaattt tttatttgtt
     2161 tgaaacttta tttaagtaat ttagttaata tttttatata gagtttgcat ctctataggt
     2221 ttataaacat caaaatatta aaagcactaa aagagggatg atatgtatga tgaaaacaac
     2281 tttagcactg taactgaaga aaaacaatat aatgaatttg agctaaatcg tgaaaattca
     2341 tttaagataa ataaggcaaa ccagaagtta aataccaaaa cttcaggttt aactccccag
     2401 tataatgaaa aagataacat cttaattaac gattatttta aggatgtaag tctggaaaca
     2461 ttactaaagc ccaagcagga gcttactctt tctgctctta tgcaaaactg tgaaaacaga
     2521 atctcttttt attcatcacc aataagtgac gttaaaatca acaaagtctg tgagtgcagc
     2581 agttcagaga gaaaaataaa gtcagtaaag caaaaaaaag aactgaacaa aagggcagtt
     2641 agaaacaaac atattattca tgcctatagt aagtttaaaa ataatcttag aaacaggttt
     2701 ataaactcta atttaaggct tgtagccagc atagccaaaa ggtttgtagg aaagggcgca
     2761 tcatttttag acctcataca ggaaggtaac atagggctta tcagggctgt tgagaagttt
     2821 gattatacca agggctacag gttttctaca tatgcagttt ggtggataaa ccagtctatt
     2881 tccagggcta tttttaatca gacaagaaca gtcaaggttc catcgtatct gctggaaaaa
     2941 tcagggaaga tctggcagac gtttataaaa ttaaaagaag aaaagggtaa ggacccatca
     3001 gcagaggaaa tttccgcaat cctaaatata tctgtagacg gtataaagag tgttataaga
     3061 acaggaaatg atgtaatcag ccttgattcc accatttata acagtgaaac aacaagctac
     3121 gtagatctgt taaaggacga taaccaggtg tttcaggatg cagagatcga taatatatca
     3181 atacctgaaa atgttaatga agctctggtt gaccttacaa caagagaaag ggaagtcatc
     3241 aagatgagat atggtatcgg gtacgatgat tcatttactc tggattacat agggcagaaa
     3301 tttggactta caagagaaag aatacggcag atagaaaaac agaccataat gaagcttaag
     3361 aaatctcata gctcagaggt actaaagagt ttgtacgata atctgcagta attctctggg
     3421 tatttattaa aagctaataa attatcctga tagttttaat aattagctga gtttgagtat
     3481 aatgattata ttccgaatgg gggaaatatg tgctcttata caaaaatcaa aagcagtctt
     3541 ggtgatgttt atgctgtttc cagttccgga gtgttatcca acctgatatt aggtttagaa
     3601 aattttgaag agtttagggc aaataataaa tctgctgaat ttaccaggtc tgatgagccc
     3661 tcagctgtat tgaaagagtt aaaatcatat ttagatggtg atcttaaaaa gtttagctat
     3721 aaactggata tatctaaagg taccaaattt caaaaaagcg tctggaaaga gcttttaaaa
     3781 attccttttg gcaaattaat aacttataaa gatatagcaa acaagcttgg aaggccaaat
     3841 tcctatcgtg ccgtaggtaa tgcttttggg gcaaatccgc ttccaatagt aatcccatgc
     3901 cacagagttg tttccaaaac tggtcttggg ggctattctt gtggtataaa ttataagaaa
     3961 aagcttttag agcacgaagg aataaattat caac
//
LOCUS       DBNGSV010000183        27921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_794743, whole genome shotgun sequence.
ACCESSION   DBNGSV010000183 DBNGSV010000000
VERSION     DBNGSV010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 27921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27921
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_794743"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC159U_06430"
     CDS             complement(<1..223)
                     /locus_tag="AC159U_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3336731.1"
                     /translation="MKRDNIYIKWFDDIDAGDIALVGGKNASLGDMYHQLCDKGVNVP
                     NGFAITADAYWHVLKSNNILDEIKKTFRGL"
     gene            complement(435..1970)
                     /locus_tag="AC159U_06435"
     CDS             complement(435..1970)
                     /locus_tag="AC159U_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HIR3336732.1"
                     /translation="MQKTKEEQYKTVNKLVFKELGIETQHEHIVFMRDDSHVCVSEGF
                     EALTRVRLSKGKKSIVASLNVITSNQLLEEGEIGLSNSAQNALGVKNGDYIEVAHLSH
                     VNSLSSVRSKIYGNKLDYRQMQSIIQDIVKGNYSNVHLSAFITACAGSNMEIDEITNL
                     TQAMISTGEQINWGTGPVVDKHCIGGLPGNRTTPIVVAVAAAAGLKIPKTSSRAITSP
                     AGTADMMETMTPVDLSINEIKKIVSKEGGCIVWGGIANLSPADDALIRVERALDLDSE
                     GQLVASVLSKKVAAGSTHVLIDIPVGKTAKVRTEEQAEKLKYFFKVVGNAVDLETKVL
                     VTDGTQPVGRGIGPALEAMDVLSVLRNESDAPKDLKHRSILIAGELLELSSRTESGKG
                     MQLAEELIISNKAYEKFMAICLAQGGFKEPEYASYKRNVFAQTAGIVGEIDNRKLANV
                     AKLAGAPQDSSAGVLFEAPVGKKVKKGQTLFTVYAQSEGELNYASSYLDSLDGSNIVS
                     IVS"
     gene            2367..3503
                     /locus_tag="AC159U_06440"
     CDS             2367..3503
                     /locus_tag="AC159U_06440"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3336733.1"
                     /translation="MEKEEFSFSDELGPLKIIHVYEPTINLKAILVVDNVAMGPSIGG
                     LRMASDVSTEECFRLARAMTLKNAAANLKHGGGKSVLFGDPAMDKDQKENLIRCMAKA
                     LRGEEEYIFGPDMGTNEECMAWVRDEIGRSVGLPREVGGIPLDEIGATGWGVRHAAEV
                     ASEFCDFKMDGARFVVQGFGAVGKHSALFLSDMGAVMVAASDSKATVYDPDGLDVNEL
                     IEHKNSGKSLSEYKKGKVLKRDDVIEIECEIWIPAARPDVITEKNVDQMKTRLIVAGA
                     NIPATTEAEKILFKKGIINVPDFVANAGGVICAAMEYQGATESSVFESIKDKIRRNTQ
                     QVLEDSANKKIAPRQAATELATARVKKAMEYRRFSAFSSAPKFV"
     gene            3515..4447
                     /locus_tag="AC159U_06445"
     CDS             3515..4447
                     /locus_tag="AC159U_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3336734.1"
                     /translation="MYQIRFHGRGGQGIKTASRILGTAFFLEGYEVQDAPRYGAERRG
                     APIFAYVRASKEQINDRGVIQKPDLVIVADDTLVPVPAAGVLDGVTKKTTVLINSHDS
                     KETWTGRLNIESQVLILPVSEESEDRAELPFIGAMCAGAGACLLGVISLKSLEKAIRT
                     ELQDLGKDIVDKNIEYAKTAFKSMNKDKGNVTETDETLADTYDSPDWVDVPFESAEQS
                     APVIHAGLTSVEVRTGLWRILRPEIDYDRCNNCWWICSTFCPDSAINVSDENIPEVDY
                     DHCKGCMICVAICPSHAIEAVPEKQSQDAKGGKL"
     gene            4447..5694
                     /locus_tag="AC159U_06450"
     CDS             4447..5694
                     /locus_tag="AC159U_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HIR3336735.1"
                     /translation="MKRQMLTGNGAAAWAARLASVDYIPAFPITPQTEIIETLSRWID
                     NKEMKGRLVTLESEHSMLTAAGSAAATGVRVFSATSSQGLLYAMEMLYTTAGWRVPFV
                     LINVSRGLAAPITMEPDHNDILAARDCGFLQIHCSTCQEVLDSTLIAYRLSEDKRVRL
                     PVIVNLDGFYLSFTREPVQLPGTKSIKNFLPPFDPEEIAFRASTPTSQAVAVLGGSPY
                     SYFRYETHLAALNALDVYDEVSDEFAELFGRSHQAVESYKSADADIVFVMMGCFATKA
                     KAAVDKLRSAGKKVGLVRPRLLRPFPEKKLSSVLANKKGVCVIDQNLSMGKGGVLHTE
                     IASALYGKNGSPSVLTSFIGGLGGRDITEEEFFEMAQITSIAADSGITPPPRLLYKED
                     ELRQMRKLQAVAHVERTELGEIQ"
     gene            5691..6611
                     /locus_tag="AC159U_06455"
     CDS             5691..6611
                     /locus_tag="AC159U_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3336736.1"
                     /translation="MSETTTEYKRWKDLPSTHLLGSGTPMCAGCGGLQALHAIYDVLG
                     EKTVFVNAAGCMTLLSVYPFTPFRGSWLYTSMASAPAGAQGIRDALDILLEQKRIPKK
                     EDLDVVVLTGDGAAYGMGLSATSGAMERDLNFIYICYDNEGYGNTGQQYSGATPHAAK
                     TATSKGPRGFAGYKKDLFSIWAAHKPAYLATIIGTEPLDLAQKIEKLKDIKGPRMLIT
                     LAPCPTGWEYDPRESIEIGRLAVKSGVWPLKEYMDGKVVHTKMPHPRIPVEEYLEKQG
                     RFSHLFKPKRNKKLLSELQAKVDEYWDNIE"
     gene            6637..8352
                     /locus_tag="AC159U_06460"
     CDS             6637..8352
                     /locus_tag="AC159U_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3336737.1"
                     /translation="MTNHKKSYYHRGGLEPLLGATIPDHFSDIAIRFADNDAVVSLHQ
                     NKRLSYLQFSKAIDKLARGIVGIGFEKSDRIGIWSTNNTQWLLLQMATARIGAVLVNI
                     NPAYRPVELEYALKQSEVQGLFIIPAFKKSDYVSMITGLIPELKKSSHSKWHSTNFPA
                     LKKVVVYDPLRPNRTARPHRGFLTWQEVIAAGSIVKKKELEQLTENLDRDDPINIQYT
                     SGTTGFPKAVVLTHHNILNNAYFSAKAMHFSEKDRLAVPVPFYHCFGMVLANLLCFSV
                     GAAIIIPSEHFDAEKTLKAIEKEKCTAIHGVPTMFIAELEHPGFKEFDLSSLRTGIMA
                     GAPCPPELMKKVIKDMHCKDILIGYGETEASPLTHLTTREDSFKRRTETVGKNLPHQE
                     VKVTDIDTGKTVAIGTDGEICFRGYHIMKGYYGNDEATKKTIDENGWLYSGDLGKMDK
                     DGYVEITGRLKEIIIRGGENIYPREIEDYLFTHPKVAEVAVFGVPDEFYGEQVMAWIE
                     LHKNESADENEIKEYCKGNIAHYKIPKYIWFVEKFPMTVTGKLQKFRMREIAIEKLEK
                     QKTLS"
     gene            8403..9572
                     /locus_tag="AC159U_06465"
     CDS             8403..9572
                     /locus_tag="AC159U_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3336738.1"
                     /translation="MENISLNLSILGILLLAGYGAHVAGRKVHIPRVTLLLLIGLLTG
                     PYFLNFIPQQISEWFPQIAHMALAMVGFLLGERFYGKDLKTTGRVVLRLSILQTVITV
                     IIVFAFLILSGAGALMALLLAGVSSATAPAATYDIVNEKKARGPLTKTLLGVVALDDA
                     WGIILFSFILVFVELSLGSGNAGFVLINGLWEVFGAMLLGVILGIPMAWLTGRIKKGE
                     PTMLEAAGFVFLCGGLALYLHVSYLLSCMVLGAIVANRAKHHTKPFRDIKGISEPFLA
                     LFFFLAGFKFELPAIMAVSSITFFYILSRFAGKYFGGLLGGSVSGADNVIQKRIGLCL
                     IPQAGVAMGMALLIAEKLPEPGVQILQIVIASTIIFEIIGPLVTVWQLKKAGEIK"
     gene            9624..10880
                     /locus_tag="AC159U_06470"
     CDS             9624..10880
                     /locus_tag="AC159U_06470"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3336739.1"
                     /translation="MNELFKSDAFENSVAQFEKVCDILELEDWIVEKFRTPQRTLIVD
                     CPIKMDDGNVKVFSGYRIQHNQTLGPCKGGIRFHPTVDMGEVASLAMNMTWKCALSEL
                     PFGGAKGGITVDPKQLSVDELQRLSRRYTAEILPIIGPNKDIPAPDLGTDAQIMSWIM
                     DTYSMSVGYTSPSVVTGKPLNIGGSLGREEATGFGVAYATEAAIHKENKEMSNIEVAI
                     QGFGNVGSFAALKLKEMGCNIVAISDSKGGIYNPFGLPVENIIELNKEGMKVSEYEEA
                     DKVTNEELIGIDCDIFIPAALGGVINDNNATKLKCNIIAEGANGPTTPGADEILEDKG
                     IKVLPDILANSGGVIVSYFEWVQGIQEYYWLKEMVFKELKTRIQNKYNKLTQYADDNR
                     LTLRSAALMMGIQRVADATRIRGIYP"
     gene            complement(10919..12277)
                     /locus_tag="AC159U_06475"
     CDS             complement(10919..12277)
                     /locus_tag="AC159U_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR3336740.1"
                     /translation="MQINHENIHRGEPVPFKVYTQNNLTKIAQLKHFNSEQLFDMQVV
                     ATVLPFRVNQHIIDSLIDWDNVPDDPMFQLTFPQRDMLEPEHFEQVAELIKSGADKQK
                     LKSVVREIHMQLNPHPAEQLTLNVPRHDGVRISGVQHKYRETVVFFPSQGQVCHSFCT
                     FCFRWPQFVNDSELRIALTEGKLLRSYLHDQKEVTDLLITGGDPMVMKTSKLAKHIEP
                     ILGSEFDHIQNIRIGTKALTYWPYRFTRDSDSDDLLRLFERVVKSGKHLALMAHFTHF
                     RELEPDATVDAIKKIQSTGAVIRSQSPVLNHINDDPAVWARLWREQVNLGVVPYYMFV
                     ERDTGARRYFELPLIRTWEIYQNAIQQVSGLGRTARGPSMSAGPGKVEIQGVSRVNGE
                     ECFILRFIQGRNPDWVDRPFFASCDPGAKWLDHLKPAFGENKFFYEDEYEQMLEKHLV
                     GF"
     gene            complement(12481..13527)
                     /gene="rtcA"
                     /locus_tag="AC159U_06480"
     CDS             complement(12481..13527)
                     /gene="rtcA"
                     /locus_tag="AC159U_06480"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR3336741.1"
                     /translation="MSEDSSLISIDGSHGEGGGQIIRTSCALAAVTGKTIEVFNIRKN
                     RRTPGLRRQHLIGIQSLNKLCNGFIEGAALGSQEILFRPGEIKPKRLKIEINTAGSIT
                     LVLQTLLLPSFLSPTPIILDFLGGATDTYFSPTIDYNRNVFFKFLEKLGLSATIKIKK
                     RGFFPSGDSRLSVKIDPGFPKSLNFLDRGSLNKVTIISGASNNLRDAKVSERQATAAS
                     KLLSSKLDISANIQIEYYDTLSTGSQINIIAEFENSIIGVDGLGSPGKRAERVGRQAA
                     KNFIKEFRSNACMDKYACDQILPYIALSKAESIFTTSEITEHTKTNMWVISHFLKRTF
                     TMEKEESRFVVRVR"
     gene            complement(13542..14567)
                     /locus_tag="AC159U_06485"
     CDS             complement(13542..14567)
                     /locus_tag="AC159U_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HIR3336742.1"
                     /translation="MTLIIWIGILICITQAGILSGLNLACFSISKLRLKIESSKKNKK
                     AAKVLELREDSNFLLTTILWANVAVNVLLTLLSESILAGVTAFMFSTLLITFFGEIIP
                     QAYFSRHALKIAYALSPMLRFYQILLYPVAKPTALILDRFLGPEAIQYFQESDLEEII
                     KMHIESTATEIDELEGIGALNFLAIDDLPITDEGEFIDPKSIITLDFQNSRAMFPNIS
                     KSVNDNFLKQILSSGKKWVIITDRRLEPRLVLNSNSLLRVVLSDADAINPYAFCHKPI
                     VVKNPNTRLGEIINQIEVYTGSLSKNVLDERVVLYWNSRKKIITSSDILDRLLSGIKK
                     QKSFSDK"
     gene            14808..15290
                     /locus_tag="AC159U_06490"
     CDS             14808..15290
                     /locus_tag="AC159U_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR3336743.1"
                     /translation="MEILTVSIEKPEEINFILGQAHFIKTVEDLHEALINSMPGIKFG
                     IAFCEASGPCLIRHSGTEPDMIELAKANALRLSAGHCFIIFLKDAFPLNVLNSIKNVN
                     EVCTIYCATANPTEVIVAESDLGRGILGVIDGYKSKGIEDDNDIEQRKLLLRKIGYKL
                     "
     gene            15478..16677
                     /locus_tag="AC159U_06495"
     CDS             15478..16677
                     /locus_tag="AC159U_06495"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR3336744.1"
                     /translation="MILENNINKVLEYKDDTSYVTTLYLKLGPDERNDFQYKRTLKNL
                     IKESKKNYSYLESDIKKYKSIENDLAMIENHINNSKNIEGLRGIAIFSCSSNDFWEVF
                     NLPLVNKDRLFFNNYPHVGELMILNDKYGNTVFLVIDKHKARFFNLTLDGVQELSGFL
                     FPGASRTHKFKPQDGKFKQRVSSSSGDRMMSQGYGEYTFNRTIENDVQQHFKYVSDRV
                     FEYSKDNKVDWLILGGSDDVVQDLSNHIHSYLKPKLLGNIKLDIDTIKTDKLFDLSMN
                     LLDDLRYKNNCKLLEEFEEKLSSGLAVNGIESTLNSLYNGQTRTLLINEGFAQPGFIC
                     PKSKLLILDNNQSPCPENQEPNKIQDIVDYIIEEAFSQKCEVDIVYDSKLKEKIDGMG
                     AILRFKL"
     gene            16742..17881
                     /locus_tag="AC159U_06500"
     CDS             16742..17881
                     /locus_tag="AC159U_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3336745.1"
                     /translation="MTNIPKQIGDKIVLNYEHLQSLFDSLINRNFKVIGPTVADNAIV
                     YGELTSANELPVGYTDEQEGGTYRLKKRNDRAAFGYNVGPHSWKKYLHPPVKKLWQAK
                     RKGTGFEIVEEQEVANKYAFIGVRSCEIHAMSIQDRVFTGGQFIDNAYKSNRENSFVV
                     AVNCGQAASTCFCDSMNTGPKVEFGFDLSLTEIIKPRGHYFVVEVGTQEALEVINEIP
                     NEYASEKDMEAADSIVDNTRGQMGREIDTSDIKELLYRNYENPRWDEVADRCLSCANC
                     TMVCPTCFCTTVEDTTDLTGDNTERVQKWDSCFTMDFSHIHGGSVRTSVKSRYRQWIT
                     HKLATWIDQFGTSGCVGCGRCITWCPVGIDITEEIDAIRKTEKEE"
     gene            17892..18365
                     /locus_tag="AC159U_06505"
     CDS             17892..18365
                     /locus_tag="AC159U_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3336746.1"
                     /translation="MAKNLKSIDSLLKEHSFFEGLDPEYLKLIAGCGSNIKFNSEDYI
                     FREGQNADHFYIIRHGKIALEISSPNRGPITIETISEGDVLGWSWLYPPYRWFLDARA
                     ISLVRAVALDGKCLREKCEDDPRLGYELMKRFSYIIAERLQGTRLRLLDLYGDGN"
     gene            18517..19212
                     /locus_tag="AC159U_06510"
     CDS             18517..19212
                     /locus_tag="AC159U_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR3336747.1"
                     /translation="MMYIYGIGEVPISITGDPADQDSVLHTIRSVGNVTSAISRLKRG
                     DTVGIRGPYGSSWPIEKAKGNDLVIVAGGIGLAPVMPVIYKIISGRENYRRVTLLYGT
                     RSSEDIIYKRELQKWRRRFDLELEITVDYDSQNWHGNVGVVTTLIPRAAFDPASTVAM
                     ICGPEIMMRYSVSELLKCGVRADMIYLSMERNMKCAVGFCGRCQYGPNFICKDGPVFK
                     YSNIENLLNIREL"
     gene            19214..20002
                     /locus_tag="AC159U_06515"
     CDS             19214..20002
                     /locus_tag="AC159U_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3336748.1"
                     /translation="MADKQKPKLAVWKFASCDGCQLSLLDCEEELLRVAGEIEIANFP
                     EASRRIIKGPYDISLVEGSITTPKDLKRIQQVRKCSKYLITIGACATAGGIQALRNFK
                     DVKDFISIVYAIPSYIQTLEKSTAIADHVRVDFELRGCPINKYQLIEVISAFLNKRKP
                     NTAPHSVCIECKMLGNVCVMVANGTPCLGPVTQAGCGALCPSYLRGCYGCFGPTETAN
                     TKALSKEWKTLGVKNEDILRAFRGINAYSDEFLKESELYEKERD"
     gene            19983..21299
                     /locus_tag="AC159U_06520"
     CDS             19983..21299
                     /locus_tag="AC159U_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR3336749.1"
                     /translation="MKKKETSNKSKTKTIKVDYLARVEGEGALYIKVKDDKVKDVKLK
                     IFEPPRFFEALLRGRRFDEAPDITARICGICPVAYQMSACHAMENAFGINVDGQLRAL
                     RRLIYCGEWIASHTLHVYMLHAPDFLGYESAIEMAKDYPKEVARGLKLKKIGNDLMSL
                     IGGREIHPINICVGGFYKLPTKSELIGIKDDLKRARDDAMQTVQLVSKFTFPDFYRDY
                     EYVALSHPNEYPINEGRIVSNKGMDIEASDYDQYFEEQHISYTNALHSVIKERGEYFV
                     GPMARYNLNFDKLSPLAKETAKDAGLEPLCNNTFKSIIIRSIEILYACDEALRIIDQY
                     EMPEKSRLEIKPSVSTGFGCTEAPRGILYHRYKMDKEGSILDAKIVPPTSQNQKTIES
                     DLCDFVDNNISLSDKELTWHCEQTIRNYDPCISCATHFLKLDIDRE"
     gene            21306..21800
                     /locus_tag="AC159U_06525"
     CDS             21306..21800
                     /locus_tag="AC159U_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR3336750.1"
                     /translation="MTEYRGNNSNILFIGIGNKYRSDDGAGLFIAERIEQIIPAGKCA
                     ISKQDGEATRLIESWKGYDIVIIADAISSGAEAGAIKRFDVKQTLLTEDIFNYSTHSF
                     SIADAVELARALDKLPEVLIVYGIEGKDFSYGDILSQKVQKNINKTAGIIIKEINNFG
                     AQHA"
     gene            21793..22059
                     /locus_tag="AC159U_06530"
     CDS             21793..22059
                     /locus_tag="AC159U_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HIR3336751.1"
                     /translation="MHEHSLIKNLIKKIESIAYENDAKTVKSFKIKLGALSHTTPEHF
                     KEHFYELSNGSIAENAEIEFIISKDMNDPLAQEIVLESVEVENN"
     gene            22055..24367
                     /gene="hypF"
                     /locus_tag="AC159U_06535"
     CDS             22055..24367
                     /gene="hypF"
                     /locus_tag="AC159U_06535"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR3336752.1"
                     /translation="MNHLEATRDHKLTRTEIKIYGRVQGVGMRPYVFRIAKKLGLRGW
                     VKNTTEGVIIEAEGRVKDICDLLTKLSNDELPNCVIRKIEKAELKYRGYKDFEIATSA
                     MTPNNSTLVQPDMATCTDCTAEIFDPNNRRYLYPFTSCSVCGPRYSIIESMPYDRANT
                     TMKNFSMCKECSIEYDDPSSRRFHTQTNCCAQCGPAVELWDSKGNPLFLKDEAIHKAI
                     LEIQNGRIVALKGLGGFHLITDAFNRDAVLKLRKRKKRKHKPFAMMFPTLGTVKLHCR
                     VSKKEATLLTSNESPIVLLKNKSHEFPSEIAPDNPYLGIMLPYTPLHHIIMEELGTPV
                     VATSGNISDEPICIDENEALERLGEIADLFLVHNRPIKRRVDDSVARVIMNKVTVIRS
                     ARGYSPHSVYIKNKVPDLLAVGGHLKNTFSVANGNYIHMSQHIGDLETLEAFNNFKDE
                     IKSFEKLFDSNPKSITCDKHPGYMSTQYAETRNLPVIPVQHHYAHVLGCMAENKIEKT
                     ALGVCWDGTGYGDDKTIWGGEFLSVSDRSYNRVAHFRTFPLPGGEKAIKEPRRAAIGI
                     LYEIFGEEIFKMNDLKPTKTFSIQQLRILKSALSKKINTNTTSSAGRMFDAVSSILGL
                     CHFNDFEGQAAMKMEFIAEAHKTEYKYNYSLNLCPDTDLSSIIIDWEQMIISIIDDLK
                     AKTHAGIISAKFHNTLTDIIVSVANYFKLKNVVLTGGCFQNKYLLESAIIHLKKEGFR
                     PYYNQMIPSNDGGLALGQIIGASYNMEQKE"
     gene            24371..24625
                     /locus_tag="AC159U_06540"
     CDS             24371..24625
                     /locus_tag="AC159U_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR3336753.1"
                     /translation="MCLAIPGKIIGITDNAPLTRRGKVSFGGLVKEVNLSFVPEADVN
                     NYVMVHVGFAISQVDEKEADQVFEYLREIDELSELKDGEI"
     gene            24638..25729
                     /gene="hypD"
                     /locus_tag="AC159U_06545"
     CDS             24638..25729
                     /gene="hypD"
                     /locus_tag="AC159U_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR3336754.1"
                     /translation="MKFIDEYRDPDSARRYSNLIKDITTKQWTIMEVCGGQTHAIVKY
                     GLDELIPDKIELLHGPGCPVCVTSVELIDKALTIALLDNVIFCSFGDMLRVPGTTGDL
                     LSVKAKGADIRIVYSPLDCLKIAKDNPSKKIVFFAVGFETTAPATAMTLYQAYNRGID
                     NFSLLVSHVLVPPAIEAILSSLNNKVQGFLAAGHVCTIMGYKEYFPIAEKYKVPIVAT
                     GFEPLDLLQGICMCIKQLEEGRHEVENQYKRSVKEDGNLKAIEIIEEVFEIVPRKWRG
                     IGEIERSGLGIKIKYEKYDAEKIFNLETYSAEESKDCISGLVLRGVKKPLECSAFGKE
                     CTPEHPLGATMVSSEGACAAYYKYRKAEV"
     gene            25777..26814
                     /gene="hypE"
                     /locus_tag="AC159U_06550"
     CDS             25777..26814
                     /gene="hypE"
                     /locus_tag="AC159U_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR3336755.1"
                     /translation="MKDFSLSCPISTDQYPNIQIAHGGGGKLMNNIIEKMFLPAFDNN
                     ILNARHDGAIVNFENTKIAFTTDSYVVSPLFFPGGDIGKLAVNGTVNDLSMCGAKPLY
                     ISIGLIIEEGFATETLWKVISSLKESAEIAGVQIVTGDTKVVEKGKGDGIYINTSGIG
                     IVDSNITIAPSNVCPGDAIILNGDIARHGIAIMTLREGLEFENEIQSDSAPLCEPVSS
                     LIGEGVDVHCMRDLTRGGLASALVEISQASKTHIQIEEASIKLSNEVRGVCEILGLDP
                     MHVANEGRFIAFVNPKHAQKALKIMNNYGEGSIIGFVKDDAQAVVTMKNLLGTNRIVD
                     MISGEQLPRIC"
     gene            complement(27434..27523)
                     /locus_tag="AC159U_06555"
                     /pseudo
     CDS             complement(27434..27523)
                     /locus_tag="AC159U_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     regulatory      27827..27918
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         8936 a         5138 c         5929 g         7918 t
ORIGIN      
        1 ctaaaccgcg aaaagttttt ttaatttcat ctaaaatatt attggatttt aaaacatgcc
       61 agtaagcatc tgccgttata gcaaaaccat ttggtacatt aactccttta tcacagagct
      121 gatgatacat atcccccagc gaggcgttct tgccccctac caaagcaata tcccctgcat
      181 ctatgtcatc aaaccattta atatatatat tatctcgttt catttgattt tcttttctct
      241 tattattatt caaatagggt gccaagagta acttcaataa tatcagttag ttatgtatga
      301 gttacaagac taaattgtct aaatatctga caacttagct cctggattta tttaatgaag
      361 cagaaatatt acacaagctg aacaggataa actaaaatat ctgcttttta ggaagtctat
      421 gtgaatacat ttatttaact tacaatacta acaatattgc ttccatctaa agaatctaaa
      481 tagctggatg cataatttag ctcgccctcc gattgtgcat atacagtaaa taaggtctgt
      541 ccctttttca cctttttgcc tacaggggcc tcgaaaagaa ctcctgccga gctgtcttgt
      601 ggagcgcctg ccaatttggc gacattggct aatttgcgat tgtcgatttc cccgactatt
      661 ccagcagttt gggcaaagac attccttttg tacgatgcat actctggttc tttgaagccc
      721 ccctgtgcca gacatatagc catgaatttc tcgtaagcct tatttgaaat aattaattct
      781 tcggcaagtt gcataccttt tccgctttct gtcctagatg aaagttcgag aagctcgcct
      841 gcaattagta tagacctatg ctttaaatct ttgggagcat ctgattcatt ccttagaacc
      901 gaaagcacgt ccattgcttc aagcgcaggg ccgatccctc ttcctacagg ctgtgtacca
      961 tctgtaacca agactttcgt ctccaagtcc actgcatttc ccactacctt aaaaaaatat
     1021 tttagttttt cggcctgttc ttccgttcta acctttgcgg tcttacctac cggaatatct
     1081 attaacacat gagtggagcc tgccgccacc tttttagata gcaccgaggc aaccagctgt
     1141 ccctcactat ctaaatcgag cgctctttca actctgataa gggcatcatc cgcaggactt
     1201 agattggcta tgcctcccca aactatgcag ccaccctcct tgctcacaat tttttttatt
     1261 tcgttaatag atagatcaac aggagtcatg gtttccatca tatcagcggt accagccggt
     1321 gaagttatag cacgagaaga tgtttttgga atttttagac cggcggctgc tgcaactgcc
     1381 actactattg gagtagtccg gttgccggga agaccgccga tacaatgttt atcaaccacc
     1441 ggaccagtgc cccagttaat ttgttccccg gtgctgatca tagcttgagt aagatttgta
     1501 atctcatcga tctccatatt gcttccggca catgccgtta tgaaggcaga aagatgtacg
     1561 ttggaataat ttccttttac gatatcctga ataattgact gcatttgcct gtagtctaat
     1621 ttgttgccat agatctttga cctcacgctg cttagagaat taacatgaga taaatgtgca
     1681 acctcgatat agtcaccatt cttaactccc agagcatttt gagcactgtt ggaaagtcct
     1741 atctcgcctt cttcaagcag ctggtttgat gtaatgacgt ttaagcttgc aactattgat
     1801 ttcttgcctt ttgagagcct tacacgtgtt agggcttcaa aaccttcgga aacacaaacg
     1861 tgactgtcgt cacgcatgaa cacaatatgt tcgtgctgtg tttctattcc cagctctttg
     1921 aacacaagct tatttactgt tttatactgt tcttctttag ttttttgcaa ctgggattcc
     1981 tgtaaattaa atgatacaca ttatatattt tttgtactac attaataata tcaaccttat
     2041 tttgtttggt catgttatta gaaattatag tatcagatta atttaaataa aatgctatta
     2101 atacagagaa tgtaaagctg tccattgtcc gtatttaaga aattattcag tttattgacg
     2161 cacaaagaat attaaacctc agatattatt ctaccgtcaa tcaaaatatt gtaatattat
     2221 cgtacactga tcatcttgtc tgattctgtg aagactctta agttgctgaa attgcttgaa
     2281 atagtttaaa tttatattgg cactcaattt gccaaatagt tatgtagttg agagtaatcc
     2341 atcatagtct taaggaaaga aatataatgg aaaaagaaga atttagtttt tcagatgagt
     2401 taggcccact caagataata catgtctatg agcccaccat taatctaaag gcaatactcg
     2461 ttgtagacaa tgtagccatg gggccctcca taggaggcct gcgtatggct tctgatgtta
     2521 gtacagagga atgcttcaga cttgccagag cgatgacact gaaaaatgct gctgcaaatc
     2581 ttaagcacgg aggcggcaaa tctgtacttt ttggcgaccc ggcaatggac aaagaccaga
     2641 aggaaaacct tatacgctgt atggctaagg ctttacgtgg agaagaagaa tatatatttg
     2701 gtcctgatat gggcaccaac gaagagtgta tggcatgggt tagggatgag ataggacgtt
     2761 cggtaggact tccaagggaa gttggaggaa taccgcttga cgaaattggt gctacgggat
     2821 ggggcgtgag acacgctgca gaagtagctt cagagttttg tgacttcaaa atggacggag
     2881 cgcgatttgt agttcagggt tttggggctg tgggaaaaca ttcagcacta tttctatctg
     2941 acatgggagc agtaatggta gcagcatctg attctaaagc tactgtatat gatccggatg
     3001 gacttgatgt aaatgaatta atagagcata agaactcagg taagagcctg tcggaatata
     3061 aaaagggtaa ggtgttaaag cgtgatgatg ttatagaaat agaatgcgag atatggattc
     3121 ctgcggcccg tcctgatgta ataaccgaaa aaaacgtaga tcaaatgaag accaggctta
     3181 ttgtggctgg ggcaaatatt cctgcaacta cagaggctga aaagattcta ttcaaaaagg
     3241 gaataattaa tgttccggac ttcgttgcaa atgcaggcgg agtaatctgc gcagcaatgg
     3301 aatatcaggg tgctacagaa tcttcagtat ttgaatccat aaaagataaa attagaagaa
     3361 atacacagca ggttttagag gactcagcaa ataaaaaaat tgccccaagg caggctgcca
     3421 ccgagcttgc tacagcaaga gtgaaaaaag ctatggaata ccgcaggttt tctgcgtttt
     3481 catcagctcc aaaatttgtt tagggaacta atatatgtat cagattagat ttcatggaag
     3541 agggggacag ggtataaaga cggcaagccg catactcggt accgcctttt ttttagaagg
     3601 ttatgaagta caggacgctc caagatacgg agcggaaaga cgcggggcgc cgatattcgc
     3661 ttacgttcgg gcatcaaagg aacagattaa cgacagggga gttatacaaa aacccgacct
     3721 tgttatcgta gcggatgaca ctttagtgcc agttcctgcc gcaggagtac ttgatggagt
     3781 cacaaaaaag actactgtac ttataaatag ccatgactct aaggaaacat ggacagggcg
     3841 attaaatatc gaatcgcagg tactgatttt gccagtcagc gaagaatccg aagaccgcgc
     3901 cgagctacca tttataggcg caatgtgcgc tggagccggg gcctgtcttt tgggagtaat
     3961 ttcacttaag tcattggaaa aggctatacg aacagaactg caagacttag ggaaagacat
     4021 agtagacaaa aatattgaat atgcaaaaac tgctttcaaa tcaatgaata aagataaagg
     4081 taatgttaca gagacggacg aaactttagc agatacgtat gattctcctg attgggtaga
     4141 tgtaccattt gaatcggctg agcagtcagc accggttata catgcaggcc ttacaagcgt
     4201 tgaagtcaga acagggcttt ggcgtattct gcgccctgaa attgactatg acaggtgtaa
     4261 taattgctgg tggatatgca gcactttttg tcccgatagc gctataaatg tatctgatga
     4321 aaacattccg gaagtggatt atgatcattg taaaggctgc atgatatgcg ttgctatctg
     4381 cccgtctcat gcaatcgaag cagtacctga aaaacagtct caggatgcaa agggaggcaa
     4441 actataatga aacggcaaat gcttacaggc aacggggcag cagcatgggc agcacgtctt
     4501 gcaagtgtag actatatacc ggctttccct attactccgc aaaccgagat aatagaaacc
     4561 ctttcgcgct ggattgataa taaagagatg aaaggcagac ttgtaacact ggaatcagaa
     4621 cattccatgc tcacggctgc cggaagcgcc gccgctacag gagtaagggt attcagtgcc
     4681 acttcaagtc aggggctttt gtatgctatg gaaatgctat acaccacagc cggctggcgc
     4741 gtgccgtttg tacttataaa tgtttcaaga gggcttgcag ctcctattac tatggaacct
     4801 gaccacaatg atatactagc agcaagggat tgtggtttct tgcagattca ctgctctact
     4861 tgtcaggagg tgctggacag cacattgatc gcttacaggc tctcagaaga caaacgagtg
     4921 cgtcttcctg tgatagttaa tttagatgga ttctatcttt cctttactcg cgagcctgta
     4981 caactaccag gtactaaatc cattaaaaat tttcttcctc cgtttgatcc cgaagagatc
     5041 gcttttcgcg caagcacacc aacaagccag gcagtggctg tgcttggtgg ctcgccctat
     5101 tcttattttc gttatgaaac acatcttgct gcactaaatg ctctggatgt gtacgatgaa
     5161 gtctcagatg aatttgcaga gctttttgga agaagtcacc aagccgtaga atcatacaaa
     5221 tccgcagatg ccgatattgt atttgtaatg atgggctgtt ttgcaacaaa ggcaaaagct
     5281 gctgtggata aattgcgttc agcaggaaag aaggttgggc ttgtcaggcc aagactttta
     5341 agaccgtttc cagaaaaaaa attaagtagt gtacttgcaa ataaaaaagg agtatgcgta
     5401 atagaccaga atctgtctat gggtaaaggt ggagtgctgc atacagaaat tgcctctgcg
     5461 ctttacggaa aaaatggcag tccttctgtg cttacaagtt ttataggcgg cctgggcggg
     5521 cgtgatataa ctgaagaaga attttttgaa atggcacaaa tcacaagcat agccgccgat
     5581 tccgggataa cgcctcctcc aagacttctt tataaagagg atgaattaag acagatgaga
     5641 aagcttcagg ccgtagcaca tgtagaacgt actgaacttg gagaaataca atgagcgaaa
     5701 caacaactga gtataaacgc tggaaggatc tgccttcaac acacctgctt ggatcaggca
     5761 cgcctatgtg tgccggctgt ggagggcttc aggccctgca cgctatatat gatgtgcttg
     5821 gcgaaaaaac agtgtttgta aatgcggccg gatgcatgac actactgtct gtttatccgt
     5881 ttactccttt tagaggttca tggttgtata cgtctatggc ctcggctcct gcaggcgcac
     5941 agggaatcag agacgcgctc gatatactcc ttgagcaaaa acgaataccc aaaaaagaag
     6001 acttagacgt tgtggttcta actggagacg gcgcagccta tggcatgggg ctttcagcaa
     6061 catcgggggc tatggaaagg gacttgaatt tcatttatat ctgctatgac aacgaaggtt
     6121 atggaaatac gggccagcag tactcgggcg ctacaccaca cgcggcaaag accgctacca
     6181 gcaaaggccc gaggggattt gctggctata aaaaagacct gttttcaatc tgggctgctc
     6241 acaaaccagc ctatcttgcc actataattg gcacagagcc tctcgatctt gcacagaaaa
     6301 tagaaaagct aaaagacata aaagggcctc gcatgttaat tactcttgcg ccctgtccaa
     6361 ccggctggga gtatgatcca agggagagta tagagatagg aagactcgcc gtgaaaagcg
     6421 gggtctggcc tttaaaggaa tatatggatg gaaaagtagt tcacacaaaa atgcctcatc
     6481 caaggattcc tgttgaagag tatctggaaa aacagggaag attttctcat cttttcaaac
     6541 caaaacgaaa taaaaagctg ctttctgagc ttcaggcaaa agtagatgag tattgggata
     6601 atatagaata aacatataat ttactcttta tttctaatga cgaatcataa aaaaagctac
     6661 tatcatcgtg gaggattgga gcctttatta ggtgctacaa ttccagatca tttttcagat
     6721 atagcgatca ggtttgctga taatgacgcg gtagtttctc tgcatcagaa taaacgactg
     6781 agctatttac agttctcaaa agcaattgac aaactcgcca gaggtattgt aggtattgga
     6841 tttgaaaaaa gcgaccgaat aggtatctgg tctactaaca atacccagtg gcttctgctt
     6901 caaatggcca cagcgcgtat tggtgctgtt ctagtaaaca taaatcctgc ttaccgcccg
     6961 gtagagctcg aatatgcgct taaacaatca gaagtacagg ggctttttat tatccccgct
     7021 tttaaaaaga gtgattatgt gtctatgatt acaggactta taccggaact aaaaaaatca
     7081 agtcatagca aatggcacag tactaatttc ccggcactta aaaaagtagt tgtatatgat
     7141 cccttgagac caaacagaac tgcccgcccc cataggggtt ttctaacctg gcaggaagta
     7201 atcgccgctg gcagcatagt taagaaaaaa gagttggagc aattaaccga aaacctggat
     7261 cgtgatgacc ctataaacat acagtacaca tccggaacta caggatttcc caaggccgtt
     7321 gtgctcacgc atcataatat tctaaacaac gcttactttt cggcaaaagc catgcatttt
     7381 tccgaaaaag accgccttgc agtaccagta cctttttatc actgctttgg gatggtgctt
     7441 gctaatctgc tctgcttttc ggttggagca gctatcatca tcccttccga acattttgat
     7501 gctgaaaaga cattaaaagc aatagaaaaa gaaaaatgca ctgcaataca tggggttcca
     7561 actatgttta tagctgagct cgaacatcct ggtttcaaag agttcgatct atcttctcta
     7621 agaacaggga taatggcagg agctccatgc ccgcctgaac ttatgaaaaa ggttataaaa
     7681 gatatgcact gcaaagatat tctcattggc tatggtgaga cggaggcttc gccgctcact
     7741 catctcacaa caagggaaga ttcatttaag aggcgtacag agacagtggg taaaaatctt
     7801 cctcatcagg aggttaaagt aacagatata gatacaggta aaaccgttgc aattggcacg
     7861 gatggagaaa tatgctttcg cggttaccat attatgaaag gatattatgg aaacgatgaa
     7921 gctaccaaaa aaactattga tgagaatgga tggctttatt cgggtgatct gggaaagatg
     7981 gacaaagatg gttatgttga gatcaccggc aggctcaagg aaattattat aaggggaggg
     8041 gaaaatattt atccacgaga aattgaagat tatttattta ctcatccaaa ggtagccgag
     8101 gtggccgtgt ttggagttcc tgatgagttt tatggtgagc aggttatggc ctggatagag
     8161 cttcataaaa atgaatcggc ggacgaaaat gaaataaaag aatattgcaa aggcaatatt
     8221 gcccattata aaattcctaa atatatctgg tttgttgaaa aatttcctat gacagtaaca
     8281 ggaaaattgc aaaaatttcg tatgcgggaa atcgcaatag aaaaactaga aaaacaaaaa
     8341 actttaagtt agatatagct ttataaaagc tatgaatttc tatttcaagc cgggaaattt
     8401 aagtggaaaa tatatctctt aatctttcta tattgggcat attgcttctg gcaggttacg
     8461 gagcacacgt agcaggtcgc aaggtgcata ttccaagagt aacgctttta cttcttatag
     8521 gacttttaac agggccgtat tttttaaact ttatacctca gcaaatatcc gagtggtttc
     8581 cgcaaatagc acatatggca cttgctatgg tgggttttct gttaggggag agattttatg
     8641 gtaaagactt aaagactacc gggcgagtcg tcttacggct ttcaatcctg caaaccgtta
     8701 taactgtgat aatagttttt gcgtttttga tacttagtgg tgcgggtgca ctgatggcgc
     8761 ttttacttgc aggagtctct tccgccactg caccagcagc gacgtacgat attgtaaatg
     8821 aaaaaaaagc aaggggaccg ctcactaaaa ccctattggg agtcgtagct cttgatgatg
     8881 catggggaat aatactgttt agctttatac ttgtcttcgt agagttatca cttggaagcg
     8941 gaaatgccgg atttgtatta ataaacggcc tttgggaagt ttttggagca atgctacttg
     9001 gagtcattct gggaatacct atggcgtggc ttacagggag aataaaaaaa ggcgaaccca
     9061 ctatgcttga agcagcagga tttgtatttc tctgcggcgg tttagcactt tatttacacg
     9121 tttcatattt gctttcatgt atggtcttag gtgctatagt tgcaaacagg gcaaaacatc
     9181 atacaaaacc atttagagac ataaagggaa taagtgagcc gtttcttgct cttttctttt
     9241 ttcttgcagg cttcaaattt gagctgcctg caataatggc tgtaagctca ataacatttt
     9301 tctatatatt aagcagattt gcaggcaaat attttggtgg gcttttagga ggcagcgtat
     9361 caggagctga taacgttatt caaaaacgaa tcggattatg tttgataccg caggcaggtg
     9421 ttgctatggg aatggctctg cttatagcag aaaaacttcc cgaaccgggc gtgcagattt
     9481 tgcaaattgt tatagctagt acaattattt ttgaaattat aggcccgctg gttactgtgt
     9541 ggcagctgaa aaaagcaggt gaaataaagt aattttatgt tcaaatttga atccacagaa
     9601 attaaaaagt accctataaa aatatgaatg aactatttaa atcagatgct tttgagaatt
     9661 cagtagcaca gtttgaaaaa gtctgcgata ttctggaact ggaagactgg attgtagaga
     9721 aattcagaac cccccagcgc actcttattg tagactgtcc tataaaaatg gatgatggaa
     9781 atgtgaaggt ttttagtggt tacaggatcc agcataatca aactctgggt ccttgtaagg
     9841 gtggtataag atttcatccc actgtggata tgggtgaagt cgcttcactt gcaatgaata
     9901 tgacatggaa gtgcgctctt tcggaactac catttggtgg agccaaggga ggtatcactg
     9961 tagatcctaa acagctatca gtagatgaac tgcaaaggct ctcaagaaga tataccgcag
    10021 aaatacttcc tatcatcgga ccaaacaaag atatacccgc tcccgattta ggaactgatg
    10081 cacaaatcat gtcatggata atggatacat acagcatgag tgttggttat acatctccat
    10141 cagttgtaac aggaaaaccg cttaatatag gaggttcact tggtagagaa gaagccaccg
    10201 gatttggagt tgcttatgct acagaagctg cgatccataa agagaacaag gaaatgtcca
    10261 atatagaggt tgcaatacag ggttttggaa atgtgggaag tttcgcagct ttaaaactaa
    10321 aagagatggg ttgtaatata gtagctataa gtgacagcaa gggtggtatt tataaccctt
    10381 tcggactgcc tgtagaaaat atcattgaat taaataaaga aggtatgaag gttagcgagt
    10441 atgaagaagc agataaagta accaacgaag aattaattgg tatagactgt gatattttca
    10501 tacccgctgc tcttggtgga gtaatcaatg acaataatgc aaccaaatta aaatgcaata
    10561 taattgcaga aggtgcaaat ggacctacaa ctcccggagc cgatgaaata ttagaagaca
    10621 agggcattaa agtcctgcca gacatactgg caaactcagg cggtgttata gtttcatact
    10681 ttgaatgggt tcagggaatc caggaatatt actggttaaa agaaatggtt tttaaggagc
    10741 ttaagacaag gatacaaaac aagtataata aactcactca gtacgccgat gataatcgct
    10801 taacccttcg ttctgctgct cttatgatgg gtatacagag agtagcagat gctacaagga
    10861 tcagaggaat ttacccttaa aaagaagttc agaaatatgt gtggcagtta atttttatct
    10921 aaaaacctac caagtgcttt tcgagcattt gctcatattc atcttcataa aagaatttat
    10981 tttctccaaa agcaggcttt aaatggtcta accattttgc cccgggatca cagctagcaa
    11041 agaaaggacg atctacccaa tcagggtttc gtccctgaat aaaacgcaat atgaaacatt
    11101 cttccccatt gacccgtgat actccctgga tctctacttt accggggccc gcactcatag
    11161 aaggccctct agccgtacgc cctagtcctg acacttgttg aattgcattt tggtaaatct
    11221 cccaagtacg aattaaagga agctcaaaat agcgtctggc tccggtatca cgttcaacaa
    11281 acatataata tggaacaacc ccaagattta cctgttcacg ccacagcctt gcccatacag
    11341 ctggatcatc attgatgtga ttcagcaccg gagattggct gcgtattaca gcccctgtgg
    11401 attgtatttt ctttatggca tcgactgtgg cgtcaggttc caattccctg aagtgtgtaa
    11461 agtgtgccat taaagccaga tgcttgcctg attttacaac tctttcaaac aacctaagca
    11521 gatcatctga gtctgaatct ctagtaaaac gataaggcca gtaagttaga gcttttgttc
    11581 ctattcttat gttttgtata tggtcgaact cgcttccaag tatgggttct atgtgcttgg
    11641 ctaatttgga cgtcttcatt accatggggt ctcccccagt gataagaagg tccgtgactt
    11701 ctttttgatc atgcagataa cttctgagaa gcttcccttc agtaagcgct atacggagtt
    11761 ctgaatcatt aacaaactga ggccagcgaa agcaaaacgt gcaaaacgaa tgacatactt
    11821 gtccctgact tgggaaaaat acaactgttt cacgatactt gtgctgaaca cctgaaattc
    11881 taacgccatc gtgacgaggg acattaagtg tgagctgctc tgcaggatga gggtttagtt
    11941 gcatgtggat ttcacgaaca actgatttta acttctgttt gtcagcacca gacttgatca
    12001 attcggcaac ttgctcaaag tgttccggtt caagcatgtc tcgctgcggg aaagtaagct
    12061 gaaacatagg gtcatcgggt acattgtccc aatcaataag actgtctata atgtgctggt
    12121 ttaccctgaa tggcagaacg gtggctacaa cctgcatatc aaataactgc tcagaattaa
    12181 agtgctttaa ttgagcaatt ttagtgagat tattttgagt gtagacttta aaagggacag
    12241 gctctcctct gtgaatgttc tcatggttaa tttgcattat ccaaccactc ctttatataa
    12301 agcaaatata gtagcatcaa tatacaaaat gaaagccctg aaggagcctc tgcatttata
    12361 ctttagcatc acaattaaat ttgtatatgt ttttatatta caattcgtat ttaatgtatc
    12421 ttcagcaata taaatacaat atattaatat ccctttataa gcatttcact ttaagtttga
    12481 ttatctaacc cttactacaa atctcgattc ttctttttcc atagtgaaag ttctttttaa
    12541 aaagtgacta attacccaca tattagtttt tgtatgttct gttatttcgg aagtcgtgaa
    12601 aatagattca gctttagata atgcaatgta aggaagtatc tggtcacagg catatttatc
    12661 catacaggca ttggatctaa attccttaat aaaatttttt gccgcctgcc tgcccactct
    12721 ttcggcccgt tttcccggac taccaagacc atcgactcca atgatcgagt tttcaaattc
    12781 ggcgataatg ttgatttgac ttcctgtaga taaggtatcg taatattcaa tttgtatatt
    12841 ggctgatatg tcgagtttcg agcttaagag cttacttgcg gcagtagcct ggcgctctga
    12901 tactttcgcg tctcttaaat tattcgaggc tccggaaata atagtaactt tattcagtga
    12961 acctctatca agaaaattaa gcgattttgg aaagcccgga tcgattttta ctgaaagtct
    13021 ggaatctccc gatggaaaaa aaccgcgctt tttgattttt attgttgcac tcagacccaa
    13081 cttttctaaa aatttaaaga aaacattacg gttgtaatct attgtcggag aaaagtaggt
    13141 atctgtagcg cctccaagaa agtcgagtat tatcggcgtg ggtgacaaaa aagaagggag
    13201 caggagagtc tgtaatacaa gggttatgct gccagctgtg tttatctcaa tcttgagccg
    13261 tttaggtttt atttctccag gcctaaataa tatttcctgt gaaccaagtg ctgctccttc
    13321 tataaatccg ttacataact tatttaaact ttgaattcct attaagtgct gtctcctcag
    13381 gcccggggtt cttctgtttt tccttatatt gaaaacctcg attgtttttc ctgtaactgc
    13441 ggctagggca catgacgttc taatgatctg tcccccaccc tctccatgag aaccatctat
    13501 agaaattaag ctgctatctt cagacacttt atagttttta tttatttatc actaaatgat
    13561 ttctgtttct ttattccgga aagcaatctg tcgagtatat ccgatgaggt aatgattttc
    13621 tttctactat tccaataaag taccacccgc tcatcaagaa cattctttga gagtgaaccc
    13681 gtgtaaacct caatctgatt aataatctca cccaaccggg tattgggatt tttgactact
    13741 ataggtttat gacaaaaagc atatggattt atagcgtccg catcagacaa tacaacccgc
    13801 aataaactat ttgagtttaa tacaagcctg ggctctaacc tgcgatcagt tattattacc
    13861 catttcttgc ccgatgatag tatctgcttt aaaaaattgt catttaccga tttgcttata
    13921 ttggggaaca ttgccctgct gttttgaaaa tccagggtga taatgctttt tgggtctata
    13981 aattcacctt catctgttat tggaagatcg tctattgcaa gaaaattgag tgccccaatg
    14041 ccctccagct catcaatttc ggttgcagtt gattcgatgt gcatctttat tatctcttct
    14101 aagtcacttt cctgaaagta ctgaatagct tcgggtccca aaaaccggtc aagaattagt
    14161 gccgtgggtt ttgctaccgg atatagtagt atctgataaa aacggagcat gggagacagt
    14221 gcataggcga tttttaaagc atgtcttgag aaatatgcct gtggtatgat ttcgccaaaa
    14281 aatgtaatca gtaatgtaga aaacataaat gctgttacac ctgctaatat agattctgag
    14341 agcaatgtta aaagaacgtt tacggctaca tttgcccaca gaattgtagt gagaagaaag
    14401 tttgagtctt ctctaagttc taaaacttta gcagcttttt tatttttttt tgaagactca
    14461 attttaagcc ttagcttgct aatactgaaa caagccagat taagaccaga taatatcccg
    14521 gcctgtgtta tacagattaa tattccgatc caaatgatta atgtcatttt attggattct
    14581 tataaaatat ttattcttat aggctatcaa gatgtgcatt gtgtttatta tttataaacc
    14641 gggttacaaa atagcattat gtaaaattat taaaatattc taaacatatt atgaatgtaa
    14701 atatcagata attcacttac ttaagaataa tatttattat atttaacata ttggtggaat
    14761 gatttttgca caaaattaga atataataac ttatggagaa cagtaagatg gaaatactaa
    14821 cagtaagtat agaaaagccc gaagaaataa attttatttt gggccaggca cattttatta
    14881 aaacggttga agacctgcat gaggctctaa ttaattccat gcctggaatt aaattcggaa
    14941 ttgccttctg tgaggcttcc gggccatgcc ttataagaca cagcggaaca gaaccagata
    15001 tgatagagct tgcaaaagca aacgcattaa ggctctcggc cgggcactgt tttattattt
    15061 tcctaaaaga cgcctttccc ttaaatgtat taaactcaat aaaaaatgtt aatgaagtct
    15121 gtacaatcta ttgcgccact gcaaacccta ctgaagtgat agtggccgaa tcagatcttg
    15181 gaaggggaat attaggcgta atagacggat acaaatcaaa aggaatcgaa gatgataatg
    15241 atattgagca aagaaaattg cttctgagaa aaatcggata caagctataa atattaaata
    15301 ttcagcacat tttaagtaca atggttatgt cttatattct tacactctac aagatcaagc
    15361 atatatttgc cgtttaatcc taataaaatc atttgtttcg gtggcacaac agttgcaaaa
    15421 tcatatatta gaaaaataaa agttctctac attactattt ctcagacagg aggcgctatg
    15481 atattagaaa acaatattaa caaagtttta gaatataaag atgacacatc gtatgtaacc
    15541 acgctatatt taaagttggg tccggatgaa agaaatgatt ttcagtataa aagaactttg
    15601 aaaaatctaa ttaaggagtc taagaaaaac tattcatatc tggaaagtga cattaaaaaa
    15661 tataaatcta tagaaaatga tttggccatg atagaaaatc atattaataa ttcaaaaaac
    15721 atagaagggt tgagggggat agcaatattt tcgtgttctt ctaatgattt ctgggaagtg
    15781 tttaacctgc cgcttgtaaa caaagacaga ctttttttta ataactatcc ccatgtcggt
    15841 gaattaatga tattaaatga taaatacggc aataccgtat tcttggtcat agataaacat
    15901 aaagcaagat ttttcaattt aaccctggat ggtgtacagg agctttcggg gtttcttttc
    15961 cccggtgcct ccaggactca taaattcaaa ccccaggacg gtaaatttaa acaacgggtt
    16021 tcatcctctt ctggtgacag aatgatgtct cagggatatg gagaatacac ttttaaccgg
    16081 actatagaga atgatgttca acaacatttt aaatacgtct cagacagggt cttcgaatat
    16141 tctaaggata ataaagttga ctggttgatt ttgggtggtt ctgatgatgt tgtccaagat
    16201 ttatcaaatc atatccactc ttacttaaag ccaaaacttc ttggcaatat caaactggat
    16261 attgatacca ttaagacaga taaactcttt gatttatcaa tgaacttgct agacgatcta
    16321 agatataaaa acaactgcaa gttattagaa gaatttgaag aaaagctgag ctctggttta
    16381 gcagtaaatg gaatagaatc caccttaaac tccctgtata acggacagac aagaacactt
    16441 ctgataaacg aaggttttgc acaacccggc ttcatttgcc ctaagtccaa actgcttatt
    16501 ctggataaca atcaaagccc atgcccggaa aatcaagaac caaacaagat acaggatata
    16561 gtcgactata taattgaaga ggcattcagc caaaaatgcg aggttgatat cgtctatgat
    16621 tccaagctta aagaaaaaat tgatggtatg ggggcaattc tgagatttaa gttataatta
    16681 gagcattagt gacaatagta aatgctcggt acaaatcttt aggtaaaccg gtcaaaaata
    16741 tatgactaat ataccaaaac aaatagggga caaaattgta ttaaattatg agcatctaca
    16801 gagccttttt gattctttaa taaatagaaa ctttaaggta attggaccaa cagttgctga
    16861 taatgcaatt gtctatggtg aactaacctc ggcaaatgag cttcctgtgg gatataccga
    16921 tgagcaggaa ggaggaactt accgtctaaa aaaaagaaat gaccgggctg cctttggtta
    16981 taatgttggt cctcactcgt ggaagaagta tttgcatccg ccggtcaaaa aactatggca
    17041 ggccaagcga aagggaacag gatttgagat tgttgaagag caagaagttg ccaataaata
    17101 tgcgtttata ggggtaagat cctgcgaaat tcatgcaatg tcaatacagg atagggtatt
    17161 tacaggcggg cagtttatag acaatgctta taaatcaaac agggaaaact catttgttgt
    17221 agcagttaac tgcggacagg cggcaagcac ttgtttttgt gattctatga acacgggccc
    17281 caaagtagaa tttggttttg acctaagctt aacagaaata attaaacccc gggggcatta
    17341 ctttgtagta gaagtcggca cccaggaggc actggaagtt ataaatgaaa ttccaaatga
    17401 atatgcctca gaaaaggata tggaagcagc ggattctata gtagataata cacgcggtca
    17461 gatgggcaga gagatagaca catcggatat taaagagctt ctttaccgta actatgaaaa
    17521 tccgcggtgg gacgaagtcg ccgacagatg tctttcctgt gccaattgca ctatggtatg
    17581 cccaacatgt ttttgcacta cggtagaaga tacaaccgac ctgactggtg ataatactga
    17641 gagggtacag aagtgggatt catgttttac catggatttt tcacatatcc atggcgggag
    17701 tgttcgtacg tcggtaaagt cgcgttatcg tcagtggata acacacaaac tcgcaacatg
    17761 gatcgaccag tttggcactt ccggatgcgt tggctgtggc aggtgcatta cctggtgtcc
    17821 ggttggaata gatataacag aagagataga cgcaataaga aaaaccgaaa aagaagaata
    17881 aggatatact ggtggcaaaa aaccttaaat caatcgatag cctgcttaaa gagcactcat
    17941 tttttgaggg ccttgatccg gaatatttaa agctcatagc agggtgcgga tcgaatataa
    18001 aattcaactc agaagattat atatttcgtg agggacagaa cgccgatcac ttttatatta
    18061 tcaggcacgg taaaatagcc ttggaaattt cgtctcccaa tcgcggcccg ataacaatag
    18121 aaactatttc tgagggagat gtgctcggct ggtcgtggtt atatcctcca tacagatggt
    18181 ttttggacgc tcgtgcaatt agtcttgttc gtgcggtagc gcttgatgga aaatgtttaa
    18241 gagaaaaatg tgaagatgac ccaaggcttg gatatgaact tatgaagagg ttttcttata
    18301 ttattgctga gcgccttcag ggaacaagac tgagactgct agacttgtac ggagacggta
    18361 attgaccttg ttatgccaga caaaattaaa agtccattta taccgacgct atatgtcatc
    18421 gatcggatca aaagggaaac acatgatacc ttcacattaa aacttttacc ccaagatata
    18481 aaatccaaaa ttagattttc tccaggacag tttaatatga tgtatatata cggtattgga
    18541 gaagtgccga tttctattac cggtgatccg gctgatcaag attcagtttt gcatacgata
    18601 cgcagtgtag gaaatgttac cagtgctatt agcagattaa aacgcggaga tacagtaggt
    18661 ataagaggcc cctatgggag cagctggcca atagagaagg ctaaaggaaa tgatcttgta
    18721 atagttgccg gtggtatagg gcttgctcca gtcatgccgg tcatatataa aattatttct
    18781 ggcagagaaa attaccgaag agtaactctg ctttatggta ccagaagttc agaagacatt
    18841 atatataaaa gggaacttca aaagtggcgc agacgttttg atttggaatt ggagataacc
    18901 gttgattatg actcgcaaaa ctggcatggc aatgtaggag tagtgacgac tcttatccca
    18961 agagcagcat ttgatccggc aagcaccgtg gcaatgattt gcggcccgga aataatgatg
    19021 agatattcgg tttcagaact gctaaaatgc ggcgtaaggg cagacatgat atatttatct
    19081 atggaacgta atatgaagtg tgctgtggga ttttgcggac gctgtcaata cggcccaaac
    19141 tttatctgta aggacgggcc agtgtttaag tatagcaaca ttgaaaatct gcttaacata
    19201 agggagctat aaaatggcag acaaacaaaa gccaaagctt gcagtatgga agtttgcatc
    19261 atgtgacggc tgtcagctta gcttgttaga ctgtgaagaa gaacttctta gagttgcggg
    19321 ggagattgaa attgcaaact ttcccgaagc ctcccgcaga ataataaaag gcccctatga
    19381 tatatcgctt gttgaaggtt caataacaac gcctaaagat ctaaaaagga ttcagcaagt
    19441 aagaaaatgc tcaaaatatt taattacaat tggcgcatgt gctacagcag gtggaattca
    19501 ggcacttaga aattttaaag atgtaaaaga ttttatttct attgtttatg cgataccatc
    19561 atatatacaa actctggaaa aatccaccgc tattgcagat catgtaagag tggattttga
    19621 gcttcgtggt tgtccgataa ataagtatca gctaattgaa gtaattagcg catttttgaa
    19681 taaacgaaaa ccaaacacgg cgccgcacag tgtctgcatc gagtgcaaga tgctgggtaa
    19741 tgtttgcgtc atggtggcta atggtactcc ttgcctgggc ccggtaacac aagctggctg
    19801 tggagcttta tgcccctctt atctgagggg ctgctatggt tgtttcggac ccacggaaac
    19861 tgctaacaca aaggcactga gcaaagagtg gaaaacactg ggtgtaaaaa atgaagatat
    19921 attgagggct tttaggggta ttaatgcata ttcagatgaa tttctaaaag aaagtgagct
    19981 ttatgaaaaa gaaagagact agcaataagt ccaaaactaa aacgataaaa gtagattacc
    20041 ttgcaagggt agagggtgaa ggtgctctat atataaaagt caaagatgat aaagttaaag
    20101 atgtaaaatt aaaaatattt gagccgccgc ggttctttga agcacttctg cggggacgaa
    20161 ggtttgatga agcccccgat ataacagcca ggatttgcgg tatatgtccg gttgcgtatc
    20221 agatgagcgc atgtcacgcc atggaaaatg cctttggtat aaatgtggat ggacaactga
    20281 gagccctcag acgtttaata tactgcgggg agtggattgc aagtcatacg cttcatgtat
    20341 atatgctgca tgcgcctgat tttctgggtt acgagagcgc aatcgaaatg gcaaaagact
    20401 atccaaagga agtggctcga ggacttaagc taaaaaagat cggaaacgac ttaatgtctc
    20461 ttattggcgg aagggaaatt catccaataa atatatgcgt tgggggtttt tataaactgc
    20521 ctacgaaaag tgaactaatc ggaattaaag atgatctcaa acgagcaaga gatgatgcaa
    20581 tgcaaacagt acaattggta tctaaattca cattccctga tttttatagg gattatgaat
    20641 atgttgccct cagtcatccc aatgaatatc caattaacga gggcagaatt gtatcaaata
    20701 agggaatgga tattgaggcc agcgattatg atcaatattt tgaagaacaa cacatctcat
    20761 acacaaatgc ccttcattcg gttattaaag aaaggggaga atactttgtt ggccctatgg
    20821 cccgttacaa tcttaatttt gataagctct caccacttgc caaagagact gctaaggatg
    20881 cagggcttga acccctatgc aacaatacat tcaaaagcat aataataagg agcatcgaga
    20941 tactgtatgc atgcgatgag gcgctgcgaa tcatagatca atatgaaatg cctgaaaaat
    21001 ctagactaga aataaaaccg tcggtgtcca caggttttgg atgcaccgaa gctccaaggg
    21061 gtattcttta tcacaggtat aaaatggaca aagaaggcag tatattggat gcaaagattg
    21121 tacccccgac ttcacaaaat caaaaaacaa tagaaagtga tttgtgcgat tttgtagata
    21181 acaacattag ccttagcgac aaagagctta catggcactg tgagcaaaca ataagaaact
    21241 acgacccctg catttcctgc gcgacacatt ttcttaaact cgatatagat agagaatgaa
    21301 aaaccatgac cgaataccga ggcaataatt caaacattct ttttataggt ataggaaata
    21361 aatacaggag tgacgacgga gcagggcttt ttatagcaga acggatcgag cagattattc
    21421 ctgcgggaaa gtgtgcaata tcaaaacaag acggtgaagc tacaagactc atcgaatcat
    21481 ggaaaggtta tgacatagtc ataatagcgg atgcaatatc ttcaggagct gaggctgggg
    21541 caattaagcg gtttgatgtt aagcaaacgc ttttaactga agatatattc aattactcaa
    21601 cacattcatt tagcatagct gatgcagttg agcttgcgcg ggctttggac aaacttccgg
    21661 aagttcttat agtctacgga atcgaaggaa aggatttttc ttatggtgat attctttctc
    21721 aaaaggttca aaaaaatatt aataagaccg ccggcataat aattaaagaa attaataatt
    21781 ttggagcaca acatgcatga gcactctctt ataaagaacc taataaagaa aatagaatca
    21841 attgcttacg aaaacgatgc taaaacagta aaaagtttta agattaaact aggtgcccta
    21901 tcccacacta cacctgagca tttcaaagaa catttctatg agctttcaaa tggcagcatt
    21961 gccgaaaatg cagagattga gttcataatt tcaaaagaca tgaatgatcc ccttgcccag
    22021 gaaatagtcc tagaatccgt agaggtagaa aacaattaat cacttagaag ctactcggga
    22081 tcacaagctt acaagaacgg aaataaaaat atacggacgc gtgcagggtg tgggcatgag
    22141 gccatatgta ttcaggattg ctaaaaaact tgggcttaga ggctgggtaa aaaatacaac
    22201 agaaggagtc ataattgaag ctgaaggcag agtgaaagac atttgtgatc ttttaacgaa
    22261 attaagtaat gatgaacttc caaactgtgt tatacgaaaa atagagaaag cagaactaaa
    22321 atatagaggc tataaagatt ttgaaatagc caccagcgcc atgactccaa acaacagtac
    22381 tttagttcag cctgatatgg ctacctgtac tgactgtacc gcagaaattt ttgatcccaa
    22441 taaccgcaga tatttatatc ctttcacatc atgttctgtg tgcgggcctc gttatagcat
    22501 tatagagtct atgccttacg acagagcgaa cacaactatg aaaaacttca gcatgtgcaa
    22561 ggagtgtagt atagagtatg acgacccttc aagcaggcgt tttcacacac aaacaaactg
    22621 ctgtgcacag tgcggacctg ctgttgagct ttgggactct aagggaaacc ctctgttctt
    22681 aaaagatgaa gctatacata aagctatatt agaaatacaa aacggaagga ttgtggctct
    22741 aaaaggatta ggcggatttc atctgataac agatgctttt aatcgggatg ctgttttaaa
    22801 attaagaaag agaaaaaaaa gaaagcacaa accttttgca atgatgtttc caactctcgg
    22861 tactgttaag cttcactgca gggtttcaaa aaaagaagct accctactca cttcaaatga
    22921 aagtccaatt gtacttctta aaaataagtc tcatgaattt ccttctgaga tagcgccaga
    22981 caatccttat cttggcatca tgctgccata caccccactt caccatataa taatggaaga
    23041 gcttgggaca ccggttgtcg ccacaagcgg caatatttct gatgaaccaa tatgtataga
    23101 tgaaaacgag gcgctcgaaa gactgggaga aattgccgat ctttttctgg ttcataacag
    23161 accaataaaa agacgagtcg atgattctgt tgcaagggtg attatgaaca aagtaactgt
    23221 aatacggtca gcaagaggct actctccaca ctcagtttat atcaaaaata aggtgccgga
    23281 tctattggcc gttggtggtc atctgaaaaa cactttttct gttgcaaatg gaaattatat
    23341 acatatgagc cagcacattg gcgatttaga aactttagag gcattcaata attttaaaga
    23401 tgagataaaa tcatttgaaa aactttttga ttctaatcca aaatcaatta cttgtgacaa
    23461 acatccgggt tatatgtcta cacaatatgc tgaaactaga aatttgcctg taatccctgt
    23521 tcagcaccac tatgcacatg tattaggctg tatggcagaa aacaaaatag aaaaaactgc
    23581 actgggagta tgctgggatg gaacaggtta tggtgatgac aaaactatct ggggcggcga
    23641 attcctaagt gtcagtgata gatcgtataa cagggtagct catttcagaa cctttcctct
    23701 tccaggcgga gaaaaagcta taaaggaacc aagacgcgct gcgataggta ttctttatga
    23761 aatatttgga gaagaaattt ttaagatgaa cgatcttaaa cccacaaaaa ctttttctat
    23821 tcagcaactg agaatattaa aatctgcgct gagcaaaaaa ataaatacta atacaacatc
    23881 gagtgcagga agaatgttcg atgcggtaag ctccattttg ggtctttgtc attttaatga
    23941 tttcgaaggg caggctgcca tgaaaatgga atttattgct gaagctcata agacagagta
    24001 taaatacaac tattcactaa atctatgtcc tgataccgat ttatcatcta taataataga
    24061 ctgggagcaa atgattataa gcataataga tgatttaaaa gcaaagacac atgcaggaat
    24121 tatatcggca aagtttcaca acacgcttac tgatattatt gtatcggttg caaattattt
    24181 taagttaaaa aatgtagtgc ttacaggagg atgttttcaa aataaatacc tgctggaaag
    24241 tgcgattatt cacttgaaga aagagggatt ccgcccctac tacaaccaga tgattccttc
    24301 aaatgacggg ggattagcac tagggcaaat aataggagcg tcatataata tggagcaaaa
    24361 ggagtaggat atgtgtctag ccatacctgg aaaaataatt ggaattacgg ataacgcccc
    24421 attaacaagg aggggtaaag taagtttcgg agggcttgta aaagaagtaa acctgtcatt
    24481 cgtacccgaa gctgatgtga acaattacgt tatggttcat gtgggttttg caataagcca
    24541 ggttgatgaa aaagaagccg atcaggtgtt tgaatatcta agagaaattg atgagctcag
    24601 tgaattgaaa gacggtgaaa tataaaatag gaaatagatg aaatttatag acgaataccg
    24661 cgatccggat tctgcccgca gatacagcaa tttaataaaa gatattacaa ccaagcaatg
    24721 gactattatg gaagtctgcg gggggcagac acatgcaata gtaaaatacg gtttagacga
    24781 gcttattcca gataaaattg aactgcttca tggccccgga tgtccagtat gcgttacatc
    24841 tgttgagctt atagataagg cgcttaccat agctttgctg gataatgtca tattttgttc
    24901 atttggcgat atgcttagag tccccggcac taccggagat ttactgtcgg taaaagcaaa
    24961 gggtgcagat attaggatag tatactcgcc tctggactgt cttaagattg caaaagataa
    25021 tccgtctaaa aaaattgttt tttttgcagt agggtttgag acaaccgccc cagcaacagc
    25081 aatgacgctg tatcaggctt acaatagggg tatcgataat ttctcgctcc tcgtatcaca
    25141 tgttcttgtg cccccggcta ttgaagcaat tctatcttcc ttgaataaca aggtgcaggg
    25201 atttttagca gccggtcatg tatgtaccat tatgggatat aaagagtact ttccaattgc
    25261 agaaaaatac aaggttccta tcgttgccac ggggtttgag cctcttgatc ttctgcaggg
    25321 aatttgcatg tgtatcaaac aactcgaaga aggcaggcat gaagtagaaa atcaatataa
    25381 aaggtcggtt aaagaagacg gcaatttgaa agctatagaa atcatagaag aagtatttga
    25441 aatagtaccc agaaagtgga gaggcatagg agaaatagaa cgaagcggtc ttggaattaa
    25501 aattaaatat gagaaatatg atgctgaaaa aattttcaat ttagaaactt attcagcaga
    25561 agagtcaaaa gactgtataa gcggacttgt tcttaggggc gttaaaaaac cactggagtg
    25621 ttctgccttt ggcaaggaat gtactccgga gcatccactt ggagccacaa tggtatcatc
    25681 cgagggggcg tgcgccgctt actataaata cagaaaagca gaagtatgaa ttaatttgta
    25741 cattacctaa atcaataaaa taatcttatg ggaaaaatta aagatttttc actatcctgc
    25801 cctatatcaa ccgaccaata ccccaatata caaattgctc atggtggagg cggtaagctt
    25861 atgaacaata ttatagagaa gatgtttctg ccggcctttg acaataacat cctaaatgcc
    25921 cgccacgacg gcgctatagt taattttgaa aataccaaga tagcatttac gactgattca
    25981 tatgtcgttt ctcctctttt cttcccgggg ggagatattg ggaaactggc tgtaaacgga
    26041 actgttaacg atctttccat gtgcggagct aagccgcttt atataagcat tgggcttata
    26101 atagaagagg ggtttgcaac agagactcta tggaaagtta ttagctcatt aaaagaatcg
    26161 gcagaaattg caggtgttca gattgtcacc ggagacacca aggtggttga aaaaggaaaa
    26221 ggtgacggga tttacattaa cacctccggg atagggatcg tagattcaaa catcactatt
    26281 gctccttcga atgtatgccc cggagatgca ataatattaa atggtgatat tgccagacac
    26341 ggcatagcaa taatgaccct aagagaagga ctggaattcg aaaatgagat acagagtgat
    26401 tctgctccac tctgtgagcc ggtttccagt cttataggtg aaggcgtaga tgttcactgt
    26461 atgagagact taacccgagg tggactagca agcgctttag tagaaatttc acaggcatca
    26521 aaaactcata tacagattga agaagcttca attaaattaa gtaacgaggt aagaggagta
    26581 tgtgaaatac tgggccttga tcccatgcat gtcgcaaatg aaggcaggtt tattgctttt
    26641 gtaaacccca agcacgctca aaaggctcta aaaattatga ataattatgg agagggttct
    26701 ataattggat ttgttaagga cgatgcacag gcggttgtta caatgaaaaa cctgctggga
    26761 acaaaccgca ttgtagatat gataagcgga gagcagcttc caaggatttg ttaagtaaac
    26821 tgccggtaat acagattgat acaaaaccat aagatatgtt catatttata aatcgcttag
    26881 gggtaatatt aatcagatat ctgatctaat aaatcaggta tatgtaaaag caaattttgt
    26941 ggcaccagaa attacaacac catctatgtg ttaatatggg gttctatcca cccacaatca
    27001 tggaataata tttcgcgccc gagaggattc gaacctctga cctacggatt agaagtccga
    27061 aaagaacaaa agagctaaac acctataaac attagataat ctaatatcta caatgagtta
    27121 cacttgtaat tttctatgca acaagtgtaa ccaattgcat tcaataccat tattttccaa
    27181 atggttctta gaatattctc agaaagaaat catgaagttt tctgcacata atttcaaaaa
    27241 attagatgat caatttaaca attagtcctg ataaataatg gctgaattcc gatgtctaac
    27301 aaagtagata tattgctctt ttctatgtgg attactagta tactagtttc tcagaagtgt
    27361 actgctcccc ttttgtcaag ctggttttaa gttggaatta tagagaacgg ggtgacaata
    27421 tgtaccccgt tgtcttcctg catacctcgg atacctttgt ccctgtctct gcctgtctta
    27481 aagcaaaggc tatctgttct tcagtaaatc ttgacttact catggcaaaa tatacctcct
    27541 gttaaatagt gttattttgc cagatttctc tttttgactg gtgcagttta cggggagaag
    27601 gtcaatacca gtcaggagct catgtgtact gtttttcctt caactcataa gaaatccttt
    27661 ttactgtgca catttctgcg caaaaggtga acagttatgc gcagatatat atgacagatc
    27721 ataatcatga ttaattaaat tgaataatta cagattatta aaaaaaaaag ttgtaattgg
    27781 tataaaaatt gtactatact tgcattgaat tacttattaa attaatttct gataatggca
    27841 caccatttga aaaaatgggc cgcaaaatca ttgcctaagg gtttggtctt ttattcctat
    27901 ggttgaaagt ttgccggata a
//
LOCUS       DBNGSV010000184         2952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1418482, whole genome shotgun sequence.
ACCESSION   DBNGSV010000184 DBNGSV010000000
VERSION     DBNGSV010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1418482"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..979)
                     /gene="istB"
                     /locus_tag="AC159U_06560"
     CDS             complement(227..979)
                     /gene="istB"
                     /locus_tag="AC159U_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR3336756.1"
                     /translation="MSEETLKKLRQMKLYGMANALETSLANTERERLSCDELISVLIE
                     AEHDDRTNRRIIRSIKNARFRYNASIEELHFDVNRNLDRTLMSRLASCKFIAKAENIL
                     ISGSTGVGKSFIASAFGNQACVEGYKVFYSNTKRLFARLKMSKADGSYLKELNKLEKI
                     DLLILDDFGLLEFDSFSRNALVDMIEDRHGRKSTIIASQLPIALWYEAIGEDTVADMV
                     MDRLVYNAYRLELAGDNLRKRQNKKHEIQMNT"
     gene            complement(1045..2658)
                     /gene="istA"
                     /locus_tag="AC159U_06565"
     CDS             complement(1045..2658)
                     /gene="istA"
                     /locus_tag="AC159U_06565"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR3336757.1"
                     /translation="MSKNLIIMSRVRRIIELFQGSTGKKAIARRLNLPLNTVRRYIWL
                     FLASGKSYEELMQMNDTELERMFIQMGNRKQVTKEERYLNLIEYFPQVEKEVKKTGVT
                     RFMMWEKYKKQHPDGYGRSQFNRYYVIWHRSKYPSAVITHKAGAKMYVDYTGKKLYIV
                     DPEKNDAIELEVFVAILGASQLIYCEASYSQDTRDFITSNVNALTYFGGSPEAIVTDN
                     LKAAVIKSSKYEPTINEAFSDFASHYTMAVLPAAPYRPTYKSLVEGAVRIIYREIFAP
                     LNNRMVFTSLESINAEIKVLLDGLNNRNFSNKNYSRRSLFNEIEVNELLELPHKAYVM
                     RDRSHGTVLKNNHVRLAQDYHYYSVPYQFIGKKVVVYYDAERVEVYYKYERIAIHKRD
                     RTAHAFTTQEEHLLAKNRGVNNWDIGKYLKRGKKVGQETLDYMHKIIENSFHKEAAFK
                     ACMGIINLRKGFNDQRINNACKRAESFKDYSYRTIENILKMGLDRVKEDEEVNGKTLP
                     RHDNIRGKDFYKDPDKDENINKDNNGNKN"
BASE COUNT          773 a          664 c          546 g          969 t
ORIGIN      
        1 tacacctccg tctgaataac cacttccaaa ataaatctct ccatgcaaac caaaagtaat
       61 tgaataataa ctacctatgc taacttcttc gcctatattg catgtgccgg gttatctgcc
      121 ccctctagac tttttttaat tgaccccacc tgtgccggcc ttaactgacc ccactaaaag
      181 ctgttcaaag accttccatt ctcgcattta aagtaataaa tatttgttac gtattcattt
      241 gtatttcatg ttttttattt tgacgtttac ggagattatc cccagcaagt tctagcctgt
      301 aggcattata caccagacgg tccatcacca tatctgcaac ggtatcttct ccaatagcct
      361 cataccaaag ggctatgggc agctgagagg ctataattgt agattttcta ccatgccggt
      421 cttcaatcat atctactaag gcatttctgc tgaagctgtc aaactccaaa agaccgaagt
      481 catcaaggat taacaagtca attttttcca gtttgtttaa ttcctttaga taggaaccat
      541 cagccttaga catcttcagt cgcgcaaaca atcttttggt attactgtaa aataccttgt
      601 atccttccac gcaggcctga ttgccgaagg cagaagcaat aaagctcttt cccacaccgg
      661 tactaccgct aatcaggatg ttctctgcct tggctatgaa cttacacgaa gcaagacgac
      721 tcattaatgt gcggtcaagg tttctgttta catcaaaatg caactcctcg atactggcat
      781 tgtacctgaa gcgggcattt ttaatgctac gtataatcct ccggttggta cgatcatcat
      841 gttctgcctc tatcagaact gagatgagct catcacaact cagacgctct ctctcggtat
      901 tggccaggct ggtttccaga gcattggcca ttccatacag cttcatttgc cgaagctttt
      961 ttagtgtctc ttcactcata acttattttt taatttattg tggtgaatag ccagagacaa
     1021 taacgatctc tggctttatt caatttaatt cttgttcccg ttattatctt tattaatatt
     1081 ctcatcttta tctggatcct tatagaagtc tttccctctg atattatcat gtctgggtaa
     1141 ggtttttcca ttgacctcct cgtcttcttt tacccggtcc aggcccatct taagtatgtt
     1201 ttcaatcgtt ctgtaactat aatctttaaa gctttccgcc cgtttgcatg cattattgat
     1261 tcgctggtca ttaaatcctt tacgcagatt aataatcccc atacaagcct taaaagcagc
     1321 ttccttgtga aaggagttct caataatctt gtgcatataa tcaagcgttt cctggcctac
     1381 cttcttcccc cgttttaagt atttaccaat atcccagttg ttcacgccac ggttcttggc
     1441 cagcaggtgc tcttcttgtg tggtaaatgc atgtgctgtc cgatctcgct tatggatggc
     1501 tatacgttcg tatttgtaat agacctctac tctttcagca tcgtagtata caacaacttt
     1561 ctttcctatg aactgataag ggacggagta gtagtggtaa tcctgagcca gccttacatg
     1621 attattcttc aatacagttc catgggaacg gtcgcgcatt acataggctt tgtgcggtaa
     1681 ttccagcagc tcgttgactt caatctcatt gaacagggaa cgccggctgt agtttttatt
     1741 actgaagttc ctgttgttga gcccgtccaa aagtacttta atctcggcat taatgctttc
     1801 cagggaggta aataccatcc tgttatttaa cggggcaaaa atctccctgt agataatcct
     1861 gactgcgcct tcaactaacg atttataggt aggacgatat ggtgctgcag gtaatactgc
     1921 catagtgtag tgagatgcaa aatcactaaa tgcttcattg atggtgggct catacttgct
     1981 gcttttaata acagcggctt tcaggttatc cgttacaatg gcttcaggtg aaccgccaaa
     2041 gtaggttagg gcatttacat tggaggtgat aaaatcccgt gtatcctggc tgtaacttgc
     2101 ctcacagtaa attagttggc ttgcaccaag aattgcgaca aatacttcca gctcaattgc
     2161 atcattcttc tcgggatcta cgatgtaaag tttcttaccg gtgtagtcga catacatttt
     2221 tgccccggcc ttgtgggtga ttactgctga tggatacttg gaccggtgcc agataacata
     2281 gtagcgatta aattgggacc tgccataacc atcagggtgc tgctttttgt atttttccca
     2341 catcataaac cgcgtaaccc ccgttttctt aacctctttc tccacttgtg ggaagtattc
     2401 gattaggttt aggtaacgtt cttctttggt tacctgcttt ctgttaccca tttggataaa
     2461 catacgctct aactccgtgt cattcatctg catcaattct tcatagcttt tccctgatgc
     2521 caagaaaagc cagatgtacc tacgcactgt atttaatggt aggtttaacc ttcttgcaat
     2581 cgctttctta ccagttgaac cctggaagag ttcaataatt cttcgtactc tgctcataat
     2641 aattaaattt ttagacatga ttaacagtat tattatcact gctaatcaaa gcattatcaa
     2701 ttaaatatgc gcgaatagaa agtgctttga actgaaggaa aaactgtgtc gttttctttt
     2761 acgtttttct cttcttaggt ggggtcaatt aaaaaaagtc tgaggggggt cagataatta
     2821 cggcaagggg ggtgcaaagt gtccggcgaa tgcaattaga ttcaatacca ccaaaataaa
     2881 acctgctagg atctttacag gatggatctg cttcaggaaa tagtttcaat aatgatatta
     2941 cgataaaatc at
//
LOCUS       DBNGSV010000185         3267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1348890, whole genome shotgun sequence.
ACCESSION   DBNGSV010000185 DBNGSV010000000
VERSION     DBNGSV010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1348890"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="AC159U_06570"
     CDS             <1..2085
                     /locus_tag="AC159U_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR3336758.1"
                     /translation="INFPLTYYVFSKFWDKGYALSKIFSILLVSYLVWLVSSVFPVFS
                     RATIFIVIIALLIFNIVLFTKYKRDISGFLKTSFHIVIVEEFLCLITLFFWSYVRSHL
                     PDINGLEKFMDFGFLNASLITKSFPPTDMWYSPLPINYYYYGHYISAMLTKLSNIDPA
                     ITYNLMIATVFSLSFTSCFSLSSNIVHLAAGKSGKDEKKRIFSRKLVLYGIISSCLLT
                     MAGNLHTVRHIWEGVNKYWYPDATRYIPFTIHEFPLYSFVVADLHAHFMNIPFVIFGL
                     STLLVIFYRRFIKFELLSIQYYILYSFLLAIFFITNSLDAAIYICFFALVLFYSNYYL
                     NRNIIKSLISALPVTAMVVLLGVLFSLPFHLHFEPFSSSIGIVTTRSEVDKLLVLWGF
                     FWFSSVVFTLAVLSTGTGRIGAVNSIAKKMLSIIANLFGLDVITRKSKSDDKSSGITL
                     MDLFVFTMIITALFLNAFPEFFYFKDIYQNDHYRANTLFKLGYQSFILYSVITAYIIF
                     RLNQLLSKNSYNIWLIVFVFGLISVMSYPYFAVKTYYNSFKNFRGLNGLSYLDKKYPD
                     DYKAILWIRENIKEPEVIIEAVGESYTDYARVSANTGLPTVVGWRVHEWLWRGSFEPV
                     GKRTEEVQAAFTTTDEKVLSDFIRKYNVKYVFIGNLERKQYKELNEQNFKDIGEEIFR
                     SGETVIYEIISN"
     gene            complement(2093..2761)
                     /locus_tag="AC159U_06575"
     CDS             complement(2093..2761)
                     /locus_tag="AC159U_06575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3336759.1"
                     /translation="MPCYNEGEVIETVIRSYHKEIIDNIDDSEFIIIDDCSSDNTPEI
                     LDRLQSDLPALRVIRPDQNGGHGKAIRLGYDNATKDYVFQVDSDNQFNPQDFPKLYEL
                     RNDFDFILGYRVNRNDPVSRLILTRILRLINFIIFGVWIKDANCPYRLIRRESLSELL
                     SEIDKEAHTPNIMLSILAKKRGLRFAEIPISHNERKTGFVFAFSWKLIKFSVTAFFQV
                     LKIR"
     gene            complement(2934..>3267)
                     /locus_tag="AC159U_06580"
     CDS             complement(2934..>3267)
                     /locus_tag="AC159U_06580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3336760.1"
                     /translation="ISIDTVIPCGLILNELISNSLKHAFPSSSVLSSDSYSNNKEITI
                     RLYKESENLITLKYEDNGIGLPDGFDFTKTDSLGLQLIVGLVKQIRGTIERVDQPGTA
                     YQINFSIA"
BASE COUNT          987 a          563 c          561 g         1156 t
ORIGIN      
        1 ataaatttcc cactgacata ttatgtgttt agtaaattct gggacaaggg atatgcgctt
       61 tcaaaaattt tttcgatatt attggtctcg tatttagtct ggttagtttc ttcggtattt
      121 cctgtatttt caagagctac tattttcatt gttattattg cgttattaat ttttaatata
      181 gtcttattta ccaaatacaa gagagacatt tcaggctttt taaaaacatc ttttcatata
      241 gtcattgttg aagaattcct ttgtctaatc acactttttt tctggagtta tgtaagatct
      301 catcttccgg acataaacgg tcttgaaaaa tttatggatt ttgggttctt aaacgcatca
      361 cttattacca aaagctttcc acctacagat atgtggtatt cgcctcttcc aataaactat
      421 tattattatg gtcactatat ttcagcaatg cttacaaagc tatcgaatat tgaccccgct
      481 attacataca acctcatgat agctacggtt tttagtctaa gctttacatc atgttttagc
      541 cttagttcga atatcgttca tcttgctgcc ggtaaatcag gaaaagatga gaaaaagaga
      601 atatttagta gaaaactcgt tttatacggc ataatatcat cctgtcttct taccatggcg
      661 ggtaatttac acactgtaag acatatttgg gagggtgtta acaagtactg gtatcctgat
      721 gccactcgct atatcccctt tactatacac gagtttcctc tatacagttt tgtagttgct
      781 gatctgcatg cacattttat gaacattcca tttgttattt tcgggctttc aaccctgctt
      841 gtaatatttt acagacgttt tattaaattc gaattattat caattcagta ctatattctc
      901 tactcctttc ttctggcaat attttttatc accaattcac tagatgcggc gatctatata
      961 tgcttttttg cccttgttct gttttattct aattattatc taaaccgaaa tattattaaa
     1021 tcacttattt ctgctctccc tgtaactgct atggtcgtac tcctgggggt gcttttttca
     1081 ctaccttttc acctgcattt tgagcctttt tcaagctcta taggaatagt aacaacaaga
     1141 tcagaagttg ataaactgtt ggttctctgg ggatttttct ggtttagttc agtcgttttt
     1201 actcttgctg ttcttagcac tggtacgggc agaattggcg ctgtcaattc aattgcaaag
     1261 aagatgctaa gtatcattgc aaatctcttt ggacttgatg taataacaag aaagtccaaa
     1321 agtgatgata aatcctccgg cataaccctt atggatttat ttgtttttac catgataata
     1381 accgctttat ttctaaacgc ttttccggaa ttcttctatt ttaaggatat ttatcaaaat
     1441 gatcactaca gggcaaatac cctgtttaag cttggatatc agagttttat tttatactct
     1501 gtaataaccg catacataat attcaggtta aatcagctac taagtaagaa ttcatataat
     1561 atatggctta tagtgtttgt ttttggtctt ataagcgtta tgagctatcc gtatttcgca
     1621 gttaagactt attacaatag ctttaaaaat ttcaggggac taaatggatt gagttacctt
     1681 gataaaaaat atccggacga ctacaaggca attctttgga taagagaaaa tattaaggag
     1741 ccggaagtaa taattgaagc tgtgggtgaa agctatactg attatgccag agtctctgca
     1801 aataccggcc tgcccactgt tgtaggatgg agggttcatg agtggttgtg gagagggagc
     1861 tttgaacctg ttggtaagag gacagaagaa gttcaggcag cgtttactac tacagatgag
     1921 aaggtattgt cggattttat tagaaaatat aatgtgaaat atgtttttat cggtaacctg
     1981 gagcgcaagc agtataagga actaaatgaa cagaacttta aggacattgg agaggaaata
     2041 ttcagatcgg gtgaaactgt aatatatgaa ataataagta attaatatct tgttagcgga
     2101 ttttcaagac ctgaaaaaaa gccgttactg aaaattttat aagcttccag ctaaatgcaa
     2161 atacaaatcc agtttttctc tcgttatgag aaattggtat ttcagcaaat ctaaggcccc
     2221 ttttcttggc aagtatagat aacataatat tgggtgtatg agcctcctta tcaatttcac
     2281 tcagtagctc agacaaactt tcccttctta taagcctgta gggacaatta gcgtctttta
     2341 tccaaactcc gaatattata aagttaatca atcttagaat acgggttaag attaatctgg
     2401 agacaggatc gtttctgttg actctataac caagaataaa atcaaaatca tttcttaatt
     2461 catataactt tgggaagtcc tgggggttga actgattatc gctgtctacc tggaaaacat
     2521 agtcttttgt agcattgtcg tatccaagcc ttatggcttt gccgtggcca ccattctgat
     2581 cgggccttat tactcttagt gctggtaaat ctgattgcaa tctgtctaga atttcgggag
     2641 tattatccga gctgcaatca tcgataataa taaattcaga atcatcaata ttatcaatga
     2701 tttctttgtg gtatgaccta ataacagttt caataacttc cccttcatta taacagggca
     2761 ttacgataga taccgacata aaaaaaataa tacatgaatc agtacgaatt cataattacc
     2821 aagaaattca gataaggtat ctatggtatt taattgataa ccatgaatat tcggaataat
     2881 taatttcagt ttttatagac tgtttttaat taataagaat atctagattt aacctatgcg
     2941 atgctgaaat ttatctgata cgccgtacct ggctggtcaa ctctttcaat agttcctctt
     3001 atttgcttta ctaaaccaac tatgagctgc agtcccagac tgtctgtttt ggtaaagtca
     3061 aaaccatcag gcaaacctat tccattatct tcatatttta atgttataag attttcagat
     3121 tccttataca gccttattgt aatctcttta ttattgctat aactatcaga gctgagcaca
     3181 ctgcttgagg gaaatgcatg tttaagcgag ttagagatta attcatttag aattaaaccg
     3241 catggaatta cagtatcgat gctaatg
//
LOCUS       DBNGSV010000186         9437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1833929, whole genome shotgun sequence.
ACCESSION   DBNGSV010000186 DBNGSV010000000
VERSION     DBNGSV010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1833929"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="AC159U_06585"
     CDS             <1..970
                     /locus_tag="AC159U_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336761.1"
                     /translation="KGDLYDIDTNTLQENIIVTVIDRQFTLRSIPNPSWIGRDDMLHV
                     GWRERPDNLWAMGPLDNLVGMQYRLDHLENLKADAFDLSVFPMVAITGAVEPFKWEPK
                     GVIHLPEDGKVDVLNLNPNALSTNNEIGGIMGIMEELAGSPKNALGIRTQGEKTAFEV
                     QTLDNAAQRVFLNKTNKFERRFLEVALNKMLEIAKRNLDTADLIRIMDDDLGVVEFMS
                     IEKEDITAKGKLIPMGARHFAARNQLIQNIMGIFNGPIAGVIAPHVSSIALAKMIEEA
                     MGLQKFQLISPNIAVAEQAETASQAQQAEQSNEVAATEPVEEDLLG"
     gene            1037..1267
                     /locus_tag="AC159U_06590"
     CDS             1037..1267
                     /locus_tag="AC159U_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336762.1"
                     /translation="MEKFLRLNTDDWNKLSKLDVINIIREHLETEKTTAIRKLIAEDN
                     FNKPSWAEHQAYLLGDVKRIEKLIQFLPDQGK"
     gene            1267..1995
                     /locus_tag="AC159U_06595"
     CDS             1267..1995
                     /locus_tag="AC159U_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336763.1"
                     /translation="MAEETIFKGDNPEATKEETVTPVVKDTQTTLTIPTELQGLIGEG
                     KKYATVEAALASVAPAQSHIATLETENKALKESVTATKTVQELIDDLRSTTAREETPS
                     KVEVNQTDIAAIVAQELAKADKNKTKVQNKTAVAQRFIKQFGEKGEEKYNKLAAEAGL
                     SVADLNILASTSPNAVFRMAGFDTQVSRDVPSTTNTGSVNSQALHGNSEEITTSKVKS
                     FNTKDVKAGWAEAGKLARKNLGLD"
     gene            2015..3010
                     /locus_tag="AC159U_06600"
     CDS             2015..3010
                     /locus_tag="AC159U_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016033201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336764.1"
                     /translation="MSQLTTNSTAFIEAEQYSQFILDNLQTFALPDGLWRDVSDFGSG
                     TTLNIKTIGTVTIQDGAEDAPLDFNSIDNNTLTLTITDYKADAWRVSDELREDGAQVE
                     ALMSMRAVESTRALAVDHESRFLAVAASESGTGQTAANVNLVNGQPHRWVAGGDGATN
                     RIMTIGDLIAAKLSMDKAGVPANGRIFIVDPVVEATFNSLTNLVNVSNNPHFEGIITD
                     GFAREHKFVKNIMGFDIWTSNFLPVKTATEALNASSYNLANDTAEIGDVVNIAMCVAD
                     DNCKPVMHAWRRMPRTEGWRDHENRGDNFQVTSRFGLGVQRTDTLIAIATHPSNY"
     gene            3028..3534
                     /locus_tag="AC159U_06605"
     CDS             3028..3534
                     /locus_tag="AC159U_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336765.1"
                     /translation="MGFETNLIRGVENHYGPRSTDKKFGGVIPGKGLIKQGVWVFDYD
                     DLPAASTTNDMVLTIPANSIILDAYFHVITAFAAGTSYDIDLVETDGTANGTGVDKMW
                     DALAVAEIDASEVAAAMKSSTHAGTNSGNIIGTKLTTAGQLAVAETGVFTAGRGRIVV
                     EYLSLPAA"
     gene            3610..4002
                     /locus_tag="AC159U_06610"
     CDS             3610..4002
                     /locus_tag="AC159U_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336766.1"
                     /translation="MSLAKQNSVDHHGQELEVGQLAFKEDGTSTVTQITNRSTGVTLN
                     KPFGQITTDNTSLAAAAEATFTVTNSTVGAKSVPVIAPASGQTAGTSIAVITAVAAGS
                     FDITLTNIHATTADTGAMVINFFVVNAV"
     gene            4035..4493
                     /locus_tag="AC159U_06615"
     CDS             4035..4493
                     /locus_tag="AC159U_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336767.1"
                     /translation="MTIQHKNIANADLHETKGVSTASDNQILIASSGVNSWGFQEYVL
                     NLDIADIVADASYFVPVPYACDIVSWSTAIDNAFATADCTLTLEIGGTLVTDSTITIT
                     QSGSAAGDVDSATPTAALSVGSGGSIEVTVSGTNTTATACHVAIIVKRTS"
     gene            4493..5254
                     /locus_tag="AC159U_06620"
     CDS             4493..5254
                     /locus_tag="AC159U_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336768.1"
                     /translation="MAAMTLLDMTQDILSDLDSDVVDSISDTVESSQVAQIIKTSYYN
                     LIDGKDWPQLKQFIQLEAATAAQPTRMKLPDNTIDVEWVKYNTKKLAGTFDKYTDIKY
                     KTPKDFIDILSLRKSDASDVDVISDDSGITLNIFNDTAPTIYTSFDDEYIVMDAYDSA
                     IDTTNLLQSKSQAFGKVQPTWTHSDTFTPDLPQQAFSYLLAEAKTACFVVLKQSENPI
                     SATQATIQRRRMSQEAFRVNNGITFANTGRKGKKI"
     gene            5254..5505
                     /locus_tag="AC159U_06625"
     CDS             5254..5505
                     /locus_tag="AC159U_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336769.1"
                     /translation="MIMYTTEAGKELELGVDPKSPLLSFRWKGGGELPVILSGLFTEE
                     GSARKAFNMWRAANTSTQEELDLGEEKSLFALAKEKVIG"
     gene            5505..7754
                     /locus_tag="AC159U_06630"
     CDS             5505..7754
                     /locus_tag="AC159U_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336770.1"
                     /translation="MPKASSEKQFNNFTKGLVTEATALSFPENASVDEDNFVLERTGR
                     RSRRLGMDYEVNYAKASTGFTDAILSSTKQSFHRWESPDGDTSVMIGIIRVFDRLWFI
                     NLLNASPSTEYLNGGTHISIDGLNSAELQVTTINNNVVIVSSDMDNPILLTYDSDNDL
                     VSQTELVLKIRDIWGVDDGLTLEERPSTLSSKHKYNLRNQGWSSRITSTCGTDAIDCT
                     FTTLGVYPSNADVWTFGKEADVSTSSTFEKYNPTFMDRNSTDNAPAPKGHYIISAFDR
                     GASRETESGLTGLPLDKENGRISTVATYAGRVFYAGVLSNITGRDDDSPFYSGFIFFT
                     QVATTTSALSRCYMSADPTSEDISDIIDTDGGTIQIPEVSKITRLVATKSSLLVFAEN
                     GVWEIFGDAGGFKATSFQVSRVSSIGTESPKSIVEVNGLMAFWSRSGIFALTQDDVSG
                     RYQSESLSLTTIQSFYNNIPNIAIQHAKGMYEEQESRIRWLYNDDADYAEDNYINKYN
                     RELVLDLTLKSFYPNTISSLAANSPAVCDYIVLPRFTSALSTTPVVAGSQEVIADQDN
                     VVSTTTIRTTRTTTFSFLALAQTDITISRYSNTSFLDWFSEDSVGVDYSSFLVTGYDI
                     FGDLMRRKYTPYVYFYFDRTEDGFTETGGNLIADNQSSCLVQAQWDWADSANSGKWGT
                     QFQAYKYKRNYIPVGDGDLFDYGHSVIITKNKLRGTGRALSLKIESETGKDLKLLGWA
                     MLMEAPARP"
     gene            7769..8128
                     /locus_tag="AC159U_06635"
     CDS             7769..8128
                     /locus_tag="AC159U_06635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3336771.1"
                     /translation="MADGPKITSVMMNMRKDNLVIGQIWLIPQSIHLYQVHLSEVIRK
                     ERGHTLDFLKEALRVGFHTMEKLEKVIAIIPINNTLTIRLTEKCKFKKEGTLKKSYMQ
                     DDKLIDQVIYSFSREDL"
     gene            8128..8673
                     /locus_tag="AC159U_06640"
     CDS             8128..8673
                     /locus_tag="AC159U_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336772.1"
                     /translation="MPFVAVAAAVAIAASVATTVNSFIAAGKQKKQLKRKERAEREHN
                     RISQKREDLKTARERVAAFREARIKRGQLLQGATNAGLGSTGGTSSVQGAEGSISSQL
                     GQNIGQLGQFQGFSQKLSALSQESADALSKFNRIGANAASLNSIFAGVGGIAKTVMAA
                     GGGGQAAGQQAAGGGGAGALT"
     gene            8673..>9437
                     /locus_tag="AC159U_06645"
     CDS             8673..>9437
                     /locus_tag="AC159U_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336773.1"
                     /translation="MIFESEIVESTDKPIPAIQPDSEVPTTEPNKPTSFMDEESEFQI
                     DDPVESKVVDIFDTSGDAMELPEPQPTPKPVLDDRRARELSFYATVEINPEDPIGSFN
                     VSRQQLITKGESDLVNAALKQAREQDEEATELAVESIIADETVHPDIRKATLQTFVST
                     GFLPTTLKERFINKTATPREDVPLAQQQIQEVILTNLQDRKNNQYSRVRQEDINKVDV
                     GTWSAFMEGMGIKFDQVRNTLGLGVSDREVAEGLNRL"
BASE COUNT         3232 a         1612 c         1948 g         2645 t
ORIGIN      
        1 taaaggtgat ctttacgaca tagatacaaa cactctgcaa gaaaatataa ttgtcactgt
       61 tattgacaga cagtttactc ttagaagtat acctaatcct agttggatcg gaagagatga
      121 tatgcttcat gtaggatgga gagaaagacc cgataacctt tgggctatgg ggccacttga
      181 caacctagta ggtatgcagt ataggcttga ccacttagaa aatcttaaag ccgatgcttt
      241 tgatctaagt gtattcccaa tggtagccat aacaggtgca gtagaaccat ttaaatggga
      301 gccaaaaggt gttattcatc ttcctgaaga tggtaaagta gatgttctaa acctgaatcc
      361 aaacgcatta tctacaaata atgagattgg tggaataatg ggtattatgg aagagttagc
      421 agggagtcca aagaatgctc ttggtattcg tactcagggt gaaaagacag cctttgaagt
      481 acaaactctg gataatgctg ctcaacgggt attcttaaat aaaactaata aatttgaaag
      541 aagattctta gaagtagctc tgaataagat gcttgaaatc gctaagagaa atctggatac
      601 tgctgacctg attcgtataa tggatgatga tttaggtgtg gtagagttta tgagtataga
      661 gaaggaagat attacagcca aaggaaaact tatcccaatg ggagcaagac actttgctgc
      721 aagaaaccaa ttaattcaaa acattatggg tatattcaat gggccgatag ctggagttat
      781 agctcctcat gtgtcatcaa ttgcacttgc taaaatgatt gaagaagcaa tgggtctaca
      841 gaaattccaa ctaattagtc ctaatatagc agttgcagag caagctgaaa cagcaagtca
      901 ggctcaacaa gcagaacaat ctaatgaggt tgctgctaca gaaccagtag aagaagacct
      961 tctcggataa taatccttga caaatttact tatatatggt ataatattat aaataagaat
     1021 cattctcatt caaactatgg aaaaatttct aagattaaat acggatgatt ggaataaatt
     1081 atccaaactg gatgtaatca atataattag agaacatcta gaaacggaga agactactgc
     1141 cattaggaaa ctaattgcag aagacaactt taataagccc tcttgggctg aacatcaagc
     1201 ctatttatta ggtgatgtga agcgtataga aaagcttatt caatttttac ctgaccaagg
     1261 aaaataatgg ctgaagaaac aatatttaag ggtgacaatc ccgaagcaac aaaagaagaa
     1321 actgtaacac cagtagtaaa agatacccaa accacactta caattccgac agaattgcaa
     1381 ggcttgatag gagaagggaa gaagtacgcg acagttgagg ctgcattagc ttcagttgca
     1441 ccagcacaat ctcatattgc tactctggaa acagagaata aagcattaaa agaaagtgtt
     1501 actgcaacaa aaactgttca ggaacttata gatgatttaa gatcgacaac agcaagagaa
     1561 gagactcctt caaaagttga agttaatcaa acagatatag ctgcaattgt agctcaagag
     1621 cttgcaaaag cagacaaaaa taaaacgaag gttcagaata aaaccgcagt tgcacaaaga
     1681 ttcattaagc aattcggtga aaaaggtgag gaaaaatata ataaactcgc tgctgaagcc
     1741 ggactatctg tagccgattt aaacatccta gctagtacat cacctaatgc cgtatttaga
     1801 atggcagggt ttgataccca agtgagtagg gatgttccgt ccactaccaa cacagggagt
     1861 gtaaactctc aggccctaca cggcaattca gaagaaataa caacatctaa agtaaaaagt
     1921 ttcaatacta aggatgttaa agctggatgg gcggaagctg gtaaattggc tcgtaaaaac
     1981 ttaggattag attaactttt aaaggaaaca ttttatgtct caattaacaa ctaactcaac
     2041 tgcttttatt gaagcagagc agtatagtca atttattctt gataatctgc aaacatttgc
     2101 acttcccgat gggttgtggc gtgacgtgtc agatttcggg agtggtacga ctttaaatat
     2161 taaaacaatt ggtacagtta caattcaaga tggtgcagaa gatgctccac ttgacttcaa
     2221 ctcaattgat aacaacacat taacacttac aatcactgac tacaaagctg atgcttggag
     2281 agtttctgat gaacttcgtg aagacggagc acaagtagaa gcacttatga gcatgagggc
     2341 tgtggaatct acaagagcat tagctgtaga ccatgaatct cgtttcttag ctgtagctgc
     2401 cagtgaaagt ggaacgggac aaaccgctgc taatgttaac ttggttaacg gacagcctca
     2461 tcgttgggta gctggtggag atggtgctac taaccgtatt atgactattg gtgatttgat
     2521 tgctgctaaa ctgtcaatgg ataaagctgg cgtacctgca aatggtcgta tctttattgt
     2581 tgatccggtt gtagaagcaa cattcaatag cctgaccaat ctggtcaacg taagtaataa
     2641 ccctcatttt gaaggtatta ttactgatgg tttcgcaagg gaacataagt tcgtgaagaa
     2701 cattatggga tttgatattt ggacttctaa tttcttacca gtaaaaactg ccactgaagc
     2761 acttaatgct tcttcttaca atctggcaaa tgatactgct gaaataggtg atgtggtcaa
     2821 tatagcaatg tgtgtagcag atgataactg caagccagtt atgcatgcat ggagacgtat
     2881 gccacgtact gaaggttggc gtgaccatga aaatcgtggt gataatttcc aagtaacttc
     2941 acgttttggg ctaggtgtac agcgtacaga tactctgatc gctattgcaa ctcacccatc
     3001 taactactaa aataggagat tattattatg ggtttcgaaa caaatttaat tcgtggcgta
     3061 gaaaatcact acgggccaag atctaccgac aagaaatttg gtggagtaat tcccggtaaa
     3121 ggattaatta agcaaggagt gtgggttttc gattatgatg atctgcctgc tgcttctact
     3181 accaatgaca tggttctgac tattcctgct aactctatta tactggatgc ttacttccat
     3241 gttataacag cttttgctgc cggaacatca tatgatattg atttggtgga aacagatggt
     3301 acagcaaacg gaacaggtgt agacaaaatg tgggatgctc tggctgtggc agaaattgat
     3361 gcctcagaag tagctgctgc tatgaagtct tcaactcacg ccggaacaaa ctcaggtaac
     3421 attattggta ctaagctaac aactgctggt caactagcgg tagctgaaac tggcgtattt
     3481 accgcaggtc gggggcgtat tgtagttgag tatctatcac ttcctgcagc ttaaggggta
     3541 atagtattta atactataaa ctaaggggag tctttgcgct ccccttttta ttaacaaaag
     3601 gtaaataaaa tgtctttagc aaaacaaaat tcagttgacc atcatggtca ggaactagaa
     3661 gtagggcaac tagcttttaa agaagatgga acctctacag taacacaaat tacaaacaga
     3721 agtacaggag taacattaaa taaaccattc ggacagatta ctactgataa tacatcatta
     3781 gctgccgcag cagaggctac attcactgtt actaatagta ccgttggtgc taagtctgtg
     3841 cctgtaatag ctcctgcatc aggacaaaca gcaggcactt ctattgctgt aattactgct
     3901 gtagcagccg gaagttttga tattaccctt accaatattc acgcaactac tgctgatact
     3961 ggagcaatgg taattaactt ctttgtagtt aacgcagttt aattaaaggg aggattttcc
     4021 tccctattta aattatgact atacaacata aaaatatagc aaatgcagat cttcacgaaa
     4081 ccaaaggagt ttcaacagct tctgataatc aaatattaat agcatcttcg ggagtaaact
     4141 cttggggttt tcaggaatat gtattaaatc ttgatatagc agatatagtt gctgatgcaa
     4201 gttattttgt accagttcct tatgcttgtg atattgtaag ctggtctact gctattgata
     4261 atgctttcgc tacagcagac tgtactctaa ccttagagat aggtggaacg cttgtaacag
     4321 attcaacgat tactattact caatcaggta gtgcagccgg agatgtagat tctgcaacac
     4381 caaccgcagc attgtctgtg ggttcaggag gttctattga agtcacagta tcaggcacta
     4441 acacaacggc tactgcttgt cacgtagcta ttattgtaaa gagaacttca taatggcagc
     4501 aatgacctta ttagacatga ctcaggatat actatcggac ttagattctg atgtagtaga
     4561 tagtatttct gatacagtgg agagttctca ggtagcacag ataattaaaa cttcatacta
     4621 caatctgatt gatggaaagg attggcctca attaaaacaa ttcattcagt tagaagctgc
     4681 taccgcagcc caacctaccc gtatgaagtt acctgataac acaattgatg tagaatgggt
     4741 taaatataat acaaagaaac ttgcaggtac tttcgataag tatacagata ttaaatacaa
     4801 aacacctaaa gacttcatag atattctttc attaagaaag agtgatgctt ctgatgtaga
     4861 tgtaatctca gatgattcag gaattactct taatattttt aatgatacag ctcctactat
     4921 ctatacctct tttgatgatg aatatatagt tatggatgca tacgattcag ctattgatac
     4981 aactaaccta ctacaaagta aatcacaggc gtttggtaaa gtacaaccaa catggacaca
     5041 ttcagataca tttacaccag acttgccaca acaggcattc agctatctct tagctgaagc
     5101 aaagacagct tgctttgtag tattaaaaca aagtgagaat cctatctctg caacacaagc
     5161 aacgatacag agacgtagaa tgtcacaaga ggcatttaga gtcaacaacg gcataacatt
     5221 tgccaacact ggaagaaaag gaaagaaaat ataatgataa tgtatacaac tgaagctgga
     5281 aaagaattag aactaggagt agaccctaaa tctccattac tatcttttag atggaaaggc
     5341 ggaggtgaac ttccagttat cctgtctggt ttatttaccg aagaaggatc tgccagaaaa
     5401 gcattcaata tgtggagagc agctaataca tcaacccaag aagagttaga tctgggagaa
     5461 gaaaaatctc tattcgcatt agctaaagaa aaagttattg ggtaatgcca aaagcaagtt
     5521 cagaaaaaca gtttaataac ttcacgaagg gattagtaac agaggccaca gccttatcat
     5581 tccctgaaaa tgcctcagta gatgaagata actttgttct tgaacggaca gggagacgct
     5641 ctcgtagact aggtatggat tatgaagtaa actatgcaaa ggcttctact ggctttacag
     5701 atgctattct atcttcaact aaacaaagtt ttcatagatg ggaaagccct gatggggata
     5761 catctgtaat gataggtatt atcagagtat ttgatcgtct ttggtttatt aatctgttaa
     5821 atgcttctcc ttcaactgaa tacctaaatg gtggtactca tatttctatt gatggactta
     5881 acagtgcaga attacaggta actacaatta acaataatgt agttatagta tcttcggata
     5941 tggataaccc tattctatta acatacgatt cagacaatga tctggtttca caaacagaat
     6001 tagtattgaa gattagagat atatggggag tagatgatgg ccttactctg gaagaaagac
     6061 catctacact atcaagtaaa cataaatata atttaagaaa tcaggggtgg agtagtcgta
     6121 taacatctac ttgtggaacg gatgctatag actgtacctt tacaactctt ggagtatatc
     6181 ctagcaatgc tgatgtatgg acttttggta aagaagcaga tgtatcaaca tcatctactt
     6241 ttgaaaaata taatcctaca ttcatggata gaaactctac agataatgcc cctgctccta
     6301 aagggcatta tattataagt gcgtttgata gaggggcttc cagagaaacg gaaagcggat
     6361 taacaggtct tcctttagac aaagaaaatg gtagaatatc tacagtggca acttatgctg
     6421 gtagagtttt ctatgccgga gtattatcaa atattacagg aagggatgat gattctcctt
     6481 tctattcagg atttatattc tttactcagg tagctacaac gacaagtgct ctatcaagat
     6541 gttatatgtc agccgaccca acatcggaag atataagtga tattattgat actgatggtg
     6601 gaacaattca gataccagaa gtcagtaaga ttacaagact tgtagcaaca aaatcttcat
     6661 tattagtatt tgcagagaat ggagtatggg aaatatttgg tgatgctggt ggatttaaag
     6721 ctacttcttt ccaagtttca agagtatctt caataggtac agaaagtcct aaaagtattg
     6781 tagaagttaa cggactaatg gctttctggt caaggtctgg tatatttgct ttaactcagg
     6841 atgatgttag tggtagatac caatcagagt ctttatcatt aaccactatc caaagtttct
     6901 ataataacat acctaatata gctattcaac atgccaaggg tatgtatgaa gaacaagaaa
     6961 gtagaataag atggttgtat aatgatgatg cggattatgc agaagataat tacataaata
     7021 aatataatag ggaactggtt ctcgacttaa cactaaagtc tttctaccct aatactatat
     7081 cttcattagc tgccaattct cctgctgtat gtgattacat cgtgctgcct agatttactt
     7141 ccgcattatc tactactcct gtagtagctg gttcacagga agtaattgcc gatcaggata
     7201 atgtagtgtc tactacaact attagaacta caagaactac tacgttctca tttctggcac
     7261 tggctcaaac agatataaca atatcaagat atagtaatac atcatttctg gattggtttt
     7321 cagaagatag tgttggtgta gattactcca gctttcttgt tacaggttac gatatatttg
     7381 gtgatttaat gagaagaaaa tatactcctt atgtatactt ctactttgat agaacagagg
     7441 acgggttcac tgaaactggt ggaaacttaa ttgcggataa tcaatctagt tgtcttgttc
     7501 aagcacaatg ggattgggct gatagtgcca atagtggaaa atggggtact caattccaag
     7561 catataaata taagagaaac tacattccag ttggtgatgg agatttattt gattatggac
     7621 atagtgttat tattacaaag aataaattaa gaggaacagg aagagcatta tcattaaaaa
     7681 tagaatcaga gacagggaaa gaccttaaac tattaggatg ggctatgctt atggaagctc
     7741 cagcaagacc ttaatgctat atatagatat ggctgatggg ccaaaaataa catctgtaat
     7801 gatgaacatg cgtaaggata accttgttat aggacaaata tggttgattc cacaatctat
     7861 tcatttatat caagtacatc tatcagaggt tatacgtaaa gaaagagggc atactttaga
     7921 ctttctaaag gaggctttaa gagttggttt tcatacaatg gaaaagctag agaaagtaat
     7981 tgctattatt cctattaaca atactcttac aataagattg acagaaaaat gcaaatttaa
     8041 gaaggaagga accttgaaga aaagttatat gcaagatgat aaactaatag atcaggttat
     8101 atactcattt agtagggagg atttataatg ccttttgttg ccgttgccgc tgccgtagcc
     8161 attgctgcta gtgttgccac tactgttaac tcctttattg ctgcgggtaa gcaaaagaaa
     8221 cagctaaaaa gaaaagaaag agctgaaagg gaacataaca gaatatccca aaaaagggaa
     8281 gacttaaaaa cagccagaga acgtgtcgct gcttttagag aggcgagaat aaaacgtgga
     8341 cagcttctac aaggagctac caatgctggg cttggatcta ctggaggtac atcatctgtt
     8401 caaggtgctg aaggaagtat ttcatctcaa ttaggacaaa acattggaca actaggacaa
     8461 ttccaaggat tcagtcagaa actatctgca ttgagtcagg agtctgctga tgctctatca
     8521 aaattcaatc gtatcggtgc aaacgctgca agccttaact caatatttgc aggtgtagga
     8581 ggtattgcta aaactgtaat ggctgctggt ggcggaggac aagcagccgg acaacaagct
     8641 gcaggtggtg gcggggccgg agcacttacc taatgatatt tgaaagtgaa atagtcgaaa
     8701 gtacagataa acctatacct gctattcagc ctgattccga agttccaaca acggaaccta
     8761 acaaacctac atctttcatg gatgaggaga gtgagtttca gattgatgat cctgtagaat
     8821 ctaaggtagt ggatatattc gatacgtctg gtgatgctat ggaattgcca gagccacaac
     8881 ccacaccaaa acctgttcta gatgatagaa gagctaggga gctttcattc tatgctactg
     8941 tagaaattaa tccggaagat ccaattggat catttaatgt atcaagacaa caacttatta
     9001 ctaaaggaga aagtgattta gtaaatgctg ctcttaaaca ggctagagaa caagatgaag
     9061 aagctacaga gcttgcagta gaaagcatta tagctgatga aactgttcat cctgatatta
     9121 gaaaagcaac cttacaaact ttcgtttcta caggattctt acccactacc cttaaagaaa
     9181 gatttataaa taaaacagct actccaagag aggatgttcc tcttgcacag caacaaattc
     9241 aagaagttat cttaactaac ctacaagata gaaaaaacaa tcaatactcc agagtaaggc
     9301 aagaagatat aaataaagtg gatgtaggca cttggtcagc atttatggaa ggaatgggca
     9361 ttaaatttga tcaggttaga aatacacttg gtcttggagt aagtgataga gaggtagcag
     9421 aaggattaaa cagatta
//
LOCUS       DBNGSV010000187         2008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1280294, whole genome shotgun sequence.
ACCESSION   DBNGSV010000187 DBNGSV010000000
VERSION     DBNGSV010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1280294"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1743)
                     /gene="ilvB"
                     /locus_tag="AC159U_06650"
     CDS             complement(40..1743)
                     /gene="ilvB"
                     /locus_tag="AC159U_06650"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR3336774.1"
                     /translation="MARMLGAEMFFETLKHEGIDVVFGLPGGYVLKVYDVMTKYLGDI
                     THVLARHEQGATHMADGYARASGKPGVVLCTSGPAATNTVTGIATAQMDSSPVVVFTG
                     QVPTQYLGSDAFQEADHIGITMPCTKHNFLVRETYDLPKAMKEAFYIATTGRPSPVLV
                     DMPKDVLIGEDEFFIPEKVNLRGYKPPTKGDPQQIKRAMNMIKKSKRPIIYGGGGIIL
                     SNASKELSDFVHRLNIPVNLTLMGLGAFPANDPLYISMLGMHGSYAANMAVHESDLVI
                     SVGGRFDDRATGGNFEKFAPNAEIIHIDIDPSSIEKNIKVDCPIIGDAKEVLKQLIEI
                     IPTDLKPRRKEWHAQIKKWQDQHPLNPHQDDDKIQTSFAIDTLSNMTKGDAIIVSDVG
                     QHQMWLAQFYRFNRPRTWINSGGLGTMGFGFPAAMGAKFAKPNERVISVCGDGSFQMN
                     MQELATAVEYKLDLTIIVFNNRHHGMVRQWQTMFFDQNYSASRFEVLPDFVMLAESFG
                     AKGMKVSRPDQVESSFKEALNMEGVVLLEVDVDCEEMVYPMIAPGAAMNEMIMDPVDV
                     A"
     gene            complement(1775..>2008)
                     /locus_tag="AC159U_06655"
     CDS             complement(1775..>2008)
                     /locus_tag="AC159U_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HIR3336775.1"
                     /translation="RGDIMADDDLVQKLLEEDEGFRNLFSEHRQLDEKVAVLENIDDL
                     SVAEELEIKQLKKLKLALKDQMQQKMKEFKKKK"
BASE COUNT          560 a          458 c          345 g          645 t
ORIGIN      
        1 aggtgtaact aaaaatcatt tcattgtaaa tagcttaact taagctacat ccacaggatc
       61 cataatcatc tcattcatag ccgctccggg ggcaatcatt ggatatacca tctcttcaca
      121 gtccacatca acctctaaaa gaaccactcc ttccatgttc agtgcttctt taaatgaaga
      181 ttcgacctga tcgggccttg aaaccttcat tccctttgct ccaaatgact ctgcaagcat
      241 aacgaaatca ggaagcacct caaagcggga tgcagagtag ttttgatcaa aaaacatcgt
      301 ctgccactgt cttaccatgc cgtggtgcct gttattaaat actattattg ttagatccaa
      361 tttatactca acagcagtag caagctcctg catattcatc tggaaactgc cgtctccaca
      421 tacagaaatc accctttcat tgggcttagc aaacttggct cccattgctg ccgggaaacc
      481 aaaacccatt gttccaagcc caccggaatt tatccaggtt cttggcctgt tgaacctata
      541 aaactgagca agccacatct gatgctggcc gacatcggat actattattg catcaccctt
      601 tgtcatattt gaaagagtat cgattgcaaa gctggtctga attttgtcat catcttgatg
      661 aggatttaga ggatgctgat cttgccattt ctttatctga gcatgccact cttttctccg
      721 aggtttcaaa tcagtaggta taatttcaat aagttgtttt aatacttctt ttgcgtcacc
      781 tattattgga cagtcaactt taatgttctt ctctattgac gacgggtcaa tatctatatg
      841 aattatttct gcattaggag cgaatttttc aaagtttcca cctgtagctc tgtcatcaaa
      901 tctgcctcca acggaaatta ccaggtctga ttcatgaact gccatatttg cggcatatga
      961 gccatgcata ccaagcatac ttatatataa tgggtcgttt gctggaaaag ctccaagccc
     1021 cataagggta aggtttacag gaatgttgag tctatgaaca aaatctgaaa gctctttgct
     1081 ggcgtttgaa agaataattc cgcctccacc atatatgatg ggtctcttag atttcttaat
     1141 catattcatg gcacgtttta tctgctgagg atctcctttt gtaggaggtt tatatcccct
     1201 taaattaacc ttttcaggaa taaagaactc atcttcaccg attaatacgt ctttaggcat
     1261 atcaactaac actgggcttg gccttcctgt ggtagcaata taaaatgctt ctttcatggc
     1321 ttttggaagg tcgtaagttt ctctgactaa aaagttgtgt ttagtacagg gcattgtgat
     1381 tccgatatga tctgcttcct ggaaagcatc actaccaaga tactgtgtgg ggacctgtcc
     1441 tgtaaaaact acaacagggg atgaatccat ttgtgcagtt gcaattcctg ttacagtgtt
     1501 agttgcagca gggccggagg tacaaagaac aactcctggt ttaccagatg cccttgcata
     1561 gccatctgcc atatgagttg caccctgttc atgtcttgca agcacatggg taatatcacc
     1621 tagatacttg gtcatgacat catagacctt aagcacatat cctccgggca gcccaaagac
     1681 tacgtctatc ccttcgtgtt taagagtctc aaaaaacatt tccgcaccaa gcattctagc
     1741 catttaatgt aaaacctcct gaattaaaat atccttattt ctttttctta aattctttca
     1801 ttttttgctg catttgatcc ttaagagcta atttcagttt ttttagctgc ttaatttcaa
     1861 gctcttcagc tacagacaaa tcatctatat tttccaaaac agcaactttt tcatctagtt
     1921 gcctgtgttc tgaaaataaa tttctaaaac cttcatcctc ttctaataac ttttgaacca
     1981 aatcatcatc tgccatgata tcacctct
//
LOCUS       DBNGSV010000188         7612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1418585, whole genome shotgun sequence.
ACCESSION   DBNGSV010000188 DBNGSV010000000
VERSION     DBNGSV010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1418585"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="AC159U_06660"
     CDS             <1..229
                     /locus_tag="AC159U_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336776.1"
                     /translation="LLHELLYQIINPKTMSKHVLQLNDKISANSNSKGGPEFWGLTEK
                     VYTSYPFASKISFARCLISSWSSKMNTLAIL"
     gene            complement(443..2449)
                     /locus_tag="AC159U_06665"
     CDS             complement(443..2449)
                     /locus_tag="AC159U_06665"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR3336777.1"
                     /translation="MAETGNISIFLSFVISIYAIIASVVGVKTRNRTLIHSASNGAIA
                     IFALLIIATISLLVSLINLDFSLKFVALNTSTDLSPIYRFTALWAGQSGSLLLWSLVL
                     AGYTFFVVKQYRNRDNHFIPYVIAILCAVSAFFLFLIAFIENPFEKLPYVPTEGRGLN
                     PILQNAYMAIHPVTLYLGYVGVTVPFAFAMGALLSGKLNDDWIKLSRKWALISWAFLS
                     IGLLLGARWAYLELGWGGYWAWDPVENAAFMPWLTATAFVHSVMIQEKKGMLKKWTIS
                     LIIITFMLSIFGTFITRSGIISSVHSFAQSNIGPLFLVFLSITLLFSSSVFFYRLKSL
                     NSENKFDSILSRESAFIFNNLLFLGAAFTVFLGTFFPILTEAIKGEKILVGSSYFNKV
                     NVPIGLVLIFLMGIGPLISWRKASKKNLINNFLYPSILGILTIVLLVILGIRDVKAIA
                     SYSLCSFVFGTILFEFIKGTKVRSSRGENYLLAFVKLVSRNKRRYGGYIVHLGVILIV
                     IGITSSSVFVDVKEASLNPGEKFDVGAYTIKYDGINQYTSDAKYGTTANLTIYKNDKY
                     AGTLHPEKNIYKYEGNREINQETEVALRSTFTDDLYIILSNFDQAGRASFRALINPMV
                     SWIWAGSLVLILGAIVTVLPEHKLRAQESSVTYSFKDKGNLAKV"
     gene            complement(2451..2864)
                     /locus_tag="AC159U_06670"
     CDS             complement(2451..2864)
                     /locus_tag="AC159U_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR3336778.1"
                     /translation="MLKGKGKFIVTIGIIAVSITYLVFAGVKETMVYYLTLDELKQRT
                     PAVYEEKVRVSGSVVEGSITNDTDGNLGFKITDGNHVIKVEYKGIVPDVFRGGVEAVV
                     EGLYKPDNTFKADLLLAKCPTKYESTESLEKGNST"
     gene            complement(2900..3034)
                     /locus_tag="AC159U_06675"
     CDS             complement(2900..3034)
                     /locus_tag="AC159U_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HIR3336779.1"
                     /translation="MVYLFWSQMIVWALLLGYVFFLDRKASGLKKQMDIIESSQKSEN
                     "
     gene            complement(3037..3693)
                     /locus_tag="AC159U_06680"
     CDS             complement(3037..3693)
                     /locus_tag="AC159U_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HIR3336780.1"
                     /translation="MKILMGFSILSMITTLYTSFIYAPNESIMGPIQRIFYFHMGTVW
                     VATVAFTVVFIASIQYLRKQSRWWDILAYCSAEIGVLFITLTIITGSIWAKPVWGTWW
                     TWDPQLTTTFILWILYIAYLILRSVAGNDSSKAKFAAVFAIIAYIDLPLVYISARLMR
                     GISPVVFGSGGGGIDSKMMVALLFAIFAFTFIYIILLIHRMEFEKIQDKLIQFKSKEI
                     "
     gene            4283..4666
                     /locus_tag="AC159U_06685"
     CDS             4283..4666
                     /locus_tag="AC159U_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3336781.1"
                     /translation="MFNNFKLLGVFVLIAVFSVSLIASAEGKGDAAKGKESYVATCAS
                     CHGVTGKGDGVASAALDPKPRNLSSAEYVSTLSDEHLFKVINDGGAAVGKSALMPAWG
                     GMLSEQDVWNVVAFIRSDLCKCQAK"
     gene            complement(5013..5213)
                     /locus_tag="AC159U_06690"
     CDS             complement(5013..5213)
                     /locus_tag="AC159U_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3336782.1"
                     /translation="MDDSKGRNFDLYIINTDGAGLERITCSAAFDRFPMFSPDGKKLV
                     WASNRNAKTRGETNIFIADWID"
     gene            complement(5217..6098)
                     /locus_tag="AC159U_06695"
     CDS             complement(5217..6098)
                     /locus_tag="AC159U_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3336783.1"
                     /translation="MINSQFNCRSIKFIKSCLVFLLVVTAATTTHSDETDQAEKLNES
                     EIHLKNITQLTFGGENAEAYFSADGKKLIFQSTRDGNAADQIYTMNTDGSDVRIVSTA
                     KGQCTCSYFMPGGNRILYSSTHHFDSQPPAPPDRSQGYVWLIHSSFDIFTSTNDGSNL
                     RQLTTSWGYDAEATVSPKGDRIFFTSIRKGDLDIYSMDTDGSNVKQLTDELGYDGGAF
                     FSADGKKIVYRSHYPSSKEEEDDYKSLLAKGLIKFSKLDVYIMYADCGNKFKVTNNDA
                     TNFCPFFAPDGKRIIFS"
     gene            complement(6134..>7612)
                     /locus_tag="AC159U_06700"
     CDS             complement(6134..>7612)
                     /locus_tag="AC159U_06700"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3336784.1"
                     /translation="SDFLPLSFSNDGKIAADLVFAGYGITAKDLNYDDYEGLDVKDKI
                     VIALRYAPEEFGKDKGYSPYTSLYNKAITAKQKGAKGIIFTTPYRKEEEKDLGGLRFD
                     FTFATSDIHATVVRRQSVEKLFSILGLDLKDTENKITAHSPNSFAMYEVRVHQQTELQ
                     RLRSNTYNVIGLIEAADKKPETKTIIIGAHYDHLGHGGVSSLAKTKKGQTQIHNGADD
                     NASGTAGLIEIAEYFASNKDSLNFNLVFIAFSGEELGLLGSSYYIKNPKVPLEQTTAM
                     INMDMIGRLRHDNLVILGTGSSPQWHDLLKSANADVGLKLKNNDTGFAPSDQSVFYAR
                     KTPVLQFFTGVHSEYHTPEDDWQKINADGQVKILNVITNLVWNINNNNLQISYSSVKG
                     DSADRPTRFNVYLGTIPDYSDESEGMKLMGVKENGPAEKAGIKGGDIIIKLGSKSIKN
                     IYDYVYALSEMKANVPTEIVVLRGGKKISLNIVAEPRRSKMN"
BASE COUNT         2298 a         1524 c         1383 g         2407 t
ORIGIN      
        1 cctgctccac gagctgttat atcagataat aaatccaaaa actatgagca aacatgtact
       61 tcaactcaat gacaaaatat cagcaaattc taactctaaa ggtggacctg aattctgggg
      121 cttgacagaa aaagtatata cttcgtatcc atttgcttct aaaatatctt ttgcaagatg
      181 ccttattagt tcttggtcat cgaaaatgaa tacccttgct attctatgag ctttattttt
      241 tctcccttca accatcttaa tcgtttctat aatattattg ttaagattta attcttatag
      301 attactaaaa cactttttta tctatttaaa ttataaagaa gctctttaat tcaagtcaat
      361 agttaaactt atatgcagta catcagatat acactactca agctgttcac tcctcctgaa
      421 tctgtgtagg tagcaagaga ttttagacct tggccaaatt gcccttatct ttaaatgagt
      481 aagtaacaga agattcttgt gctcttagct tatgttcggg taataccgtt acaatagccc
      541 ctaaaattag aacgaggctg cctgcccata tccagcttac catggggttt atcagagccc
      601 taaagcttgc acgtcctgcc tgatcgaaat ttgataaaat gatatataaa tcatctgtaa
      661 aagttgatct taaagcaacc tcggtttcct ggttgatttc tctgtttcct tcatacttgt
      721 atatattttt ttcaggatgc aatgtgccag catatttatc gtttttatat atagtaagat
      781 tagcagtagt accgtatttg gcatcagatg tatactgatt tatgccatcg tattttatgg
      841 tataggcccc aacatcgaat ttctcaccgg gattaagaga ggcttctttg acatctacaa
      901 atactgaaga ggaagtaatt cctatgacta ttaaaataac tcccagatgt actatgtatc
      961 ctccgtacct tctcttgttt cgtgatacta gcttaacaaa agccaacaaa tagttttctc
     1021 ctctagaaga tctgacctta gtacctttaa tgaactcaaa gagtattgtg ccaaaaacaa
     1081 agctacatag tgaataagaa gcaatggcct taacatccct gatgccaagg ataacaagca
     1141 aaactatggt caaaattccc aagatcgatg gataaagaaa attgtttatt aagttcttct
     1201 tagatgcttt tctccatgat ataagcggtc cgattcccat aaggaatatc agaactagac
     1261 ctattggcac attaaccttg ttgaaatagg aagagcccac aagaattttt tcgcctttta
     1321 tggcttctgt taaaatcgga aaaaatgtac ctagaaatac ggtaaaggcg gcccccaaaa
     1381 acaataggtt gttaaatata aaggcgcttt ctctagaaag aatcgagtcg aatttgtttt
     1441 cgctattcag actttttaac ctgtaaaaga atactgacga cgaaaataaa agcgtgatgg
     1501 ataaaaacac caaaaacagt gggcctatgt tggactgggc aaaagaatga actgaagaga
     1561 taataccgct tctggttata aaagtaccga atattgagag cataaaagtt attattataa
     1621 gtgaaatagt ccacttcttt aacatcccct ttttttcttg aatcattaca gaatgcacga
     1681 aagcagtcgc tgtaagccac ggcatgaaag ccgcattttc tacaggatcc catgcccagt
     1741 atccacccca tcccagctcc agataagccc atcttgcacc gagcaaaagt ccgatgctta
     1801 aaaacgccca ggatatcaac gcccattttc ttgaaagttt gatccagtca tcatttaatt
     1861 taccgctgag cagcgctccc attgcaaatg caaacggcac agttactcct acataaccta
     1921 aatacaaggt taccggatgt attgccatgt atgcattttg caaaattgga ttgagccctc
     1981 taccctcggt tggtacatat ggaagctttt cgaaagggtt ttcaataaat gcaatgagga
     2041 aaagaaaaaa cgccgatacg gcacaaagta tggctattac atatgggata aaatgattgt
     2101 ctctattcct gtactgtttg actacaaaaa aagtatagcc tgcaagaacc aaagaccaaa
     2161 gcagaagtga tcctgattga cccgcccaca gagccgtaaa tctgtatatt ggcgaaaggt
     2221 cggtacttgt atttaatgcg acaaacttaa gactaaaatc aagattaata agagaaacca
     2281 acagtgatat tgttgctata atcagtagag caaatatagc aatagcgcca ttgctggcgc
     2341 tatgaattag ggttctattt cttgttttga caccgacaac cgacgcaatt atcgcataaa
     2401 ttgaaataac aaaggataga aaaatggaga tattgccagt ttctgccatc ttatgtgcta
     2461 ttaccctttt ctaatgactc cgtagactca tacttggttg gacattttgc aagcagtaaa
     2521 tctgctttga atgtgttatc aggtttatat aaaccttcta ctactgcttc tacgccgccc
     2581 ctgaaaacat cgggcacaat gcctttgtat tcgaccttta taacatgatt gccgtctgtt
     2641 atcttaaagc ccagatttcc gtctgtatca tttgttatag agccctcaac cacagatcca
     2701 gatactctta ccttttcttc atacactgcc ggggttcttt gcttgagctc atcaagtgtt
     2761 aaatagtaaa ccattgtttc tttaacaccc gcaaacacaa gatatgttat agaaacggct
     2821 ataataccga tagttacaat aaattttcct ttacctttta gcatatcaga atctccataa
     2881 tttatagcat ataatcattc taattctcac ttttttgtga gctttcgata atatccattt
     2941 gcttctttaa gcccgaggcc tttctatcca ggaaaaaaac ataaccgagc aataaggccc
     3001 agactatcat ctgagaccaa aacaggtaaa ccatagctat atctcctttg acttaaattg
     3061 tattaattta tcttgaatct tttcaaattc cattctgtgt atcagtaata ttatatatat
     3121 aaaagtaaat gcaaaaatcg caaataataa cgcaaccatc atctttgaat ctatgccccc
     3181 tccgccagag ccaaaaacaa ccggagatat gcctctcata agtctagcag aaatatacac
     3241 taaaggtaaa tctatatatg cgattattgc aaataccgct gcaaatttgg cttttgaaga
     3301 atcatttcca gcaacagatc ttaatattaa atatgcaatg tatagaatcc acaatataaa
     3361 cgtagttgtg agctgtgggt cccaggtcca ccaggtgccc catacaggtt ttgcccatat
     3421 gctgccggtt ataatagtaa gcgtaatgaa gagcactcca atttctgccg aacagtaagc
     3481 cagtatatcc caccacctgc tttgttttct gagatactga atactggcta taaatacgac
     3541 tgtaaaagct actgtagcga cccacacggt tcccatatgg aaataaaaaa tcctctgaat
     3601 tggtcccatg atactttcat tgggtgcata gataaaagaa gtgtatagag tggtaatcat
     3661 gcttaagata ctaaatccca ttaatatttt catagtctat taattttatt gttatttatt
     3721 aaatttattg tttttattaa aagtatcata aatacaataa aaatgtatct actacaaata
     3781 taaattgtta agatagaaat ttatttcttc tttcaatatt ccttaatatt tcaatagtca
     3841 atagacatta tattaactaa tttgattgca aaatatatgc caggggcata ggatatgtgt
     3901 aatatttgac ttgcttagag tagtgagtag tcactcattt aaggaattag attatattgt
     3961 aaactatata gaaaacaagg agattataaa tctaatattg taagatatca gcatatctga
     4021 caccagatcc acagagcaat atatgcttta tttgcatgct gatagcaagt tgacatccat
     4081 taaataattt gtcggtagta tgtagataaa agttatttat tattacaaat cttacaaaat
     4141 cttcttatta atattaagag ttttgtttta tctcatatat tctgaaactt tatattaata
     4201 tagttgttta tataaataac atcagtttaa tgatcgacaa taaaattata tcattatcaa
     4261 ttattaaagg aggtgtttga ttgtgtttaa caatttcaag ttactaggag tgtttgtttt
     4321 aatagccgtt ttcagtgtct ccctcattgc atctgccgaa ggtaaaggtg atgcagcaaa
     4381 gggcaaagag tcatatgttg ccacctgtgc gtcttgccat ggcgttaccg gcaaaggaga
     4441 cggagtggcc tcagcagctc tcgatcctaa accaaggaat ttgagcagcg ctgagtatgt
     4501 atccactctt tctgacgaac atctgtttaa ggtaataaac gatggcggcg cagcagtcgg
     4561 caagtctgca ctcatgcctg cttggggtgg gatgctttca gaacaggacg tatggaatgt
     4621 agtagctttt ataaggtctg atctttgtaa atgtcaggca aaatagcaat aatttaagcc
     4681 taataggagg gacaggctga tctacgtacc tcattgttcc aatattgtac accaagcatc
     4741 ctatactaaa tgaattaagg aagccgcatg attatcgcgg caaatgcgga gaatgttctt
     4801 ttaacgcagt atgctgagag tcaagggcaa aagcatttgc tgtgagcgga gactacatcg
     4861 ggcagtatcc aatctgttat ttcgatgagc aaatattaaa acagtcaaaa ctacagattt
     4921 aatagtatag tacgtgaagg gatagtagca tattaagggc taagcagatt ggacaaataa
     4981 tcagaaaagt ttatctggta taacaatatg ccctaatcaa tccagtcggc tatgaaaatg
     5041 ttagtctcgc ctcttgtctt ggcgtttctg ttagatgccc agacaagctt cttgccgtct
     5101 ggcgaaaaca taggaaacct gtcaaaagca gctgagcaag tgatccgctc caatccagcc
     5161 ccatctgtat tgatgatgta aagatcaaaa ttgcgccctt tggaatcatc catattttat
     5221 gaaaaaatta ttctctttcc gtcaggagca aagaacgggc agaaattagt tgcatcgttg
     5281 tttgtcactt taaatttatt cccacaatcc gcatacatta tatagacatc aagcttgctg
     5341 aatttaataa gcccttttgc aagcagactt ttgtaatcgt cttcctcttc tttgctagac
     5401 ggataatgtg acctgtacac gattttcttc ccgtcggcag aaaagaatgc cccgccgtca
     5461 tatcccagct cgtcagtcaa ttgtttaaca ttgcttccgt cggtgtccat tgaatatata
     5521 tcgaggtcac cctttctaat cgacgtaaaa aagattctat cccctttagg agaaactgta
     5581 gcttcggcat cataacccca cgaagttgta agttgtctta aattgctacc atcatttgtt
     5641 gatgtaaata tgtcgaaaga tgaatggata agccaaacat atccctgaga cctgtctgga
     5701 ggtgctggag gctgagagtc aaaatgatgg gtggatgaat acagtattct attgccacca
     5761 ggcatgaaat aagaacacgt gcactgtcct tttgctgtgc ttacgattct tacgtcacta
     5821 ccgtccgtat tcatagtgta aatctggtct gcggcattgc cgtcacgggt tgactggaat
     5881 attaacttct tgccgtctgc agaaaaatag gcttcggcgt tttccccacc aaaagtgagc
     5941 tgtgttatgt tttttaggtg tatttccgat tcattgagct tctcggcctg gtctgtttca
     6001 tccgaatgtg tagtagttgc cgctgtgaca accaataaaa aaacaagaca acttttaata
     6061 aatttaattg atctgcaatt gaattgtgaa ttgatcattg gttcttactc ctgttcgggt
     6121 tgtgatataa gtatcagttc attttgctcc gtcttggctc tgcaactata tttagactta
     6181 ttttcttacc ccctcttaaa acaacgatct ccgtaggtac gtttgccttc atttcactca
     6241 gtgcatatac atagtcgtat atatttttta tacttttact gccaagcttg atgatgatat
     6301 ctccaccctt tatacctgct ttttctgcag gaccgttttc tttcaccccc ataagcttca
     6361 tcccctctga ttcatcggag tagtctggaa tcgtaccgag ataaacattg aaccttgtag
     6421 gtctgtctgc gctatcaccc ttgacgctcg agtatgatat ctgcaggtta ttgttgttaa
     6481 tgttccaaac caggttagta attacgttta aaatttttac ctgtccgtcc gcattgattt
     6541 tctgccagtc gtcttcgggt gtgtgatatt cactgtgcac accggtaaag aattgcagca
     6601 cgggggtttt ccgggcataa aagacggact ggtcgcttgg ggcaaatccc gtgtcgttgt
     6661 ttttgagttt aagaccgaca tccgcgtttg cactcttaag cagatcgtgc cactgaggag
     6721 acgatcccgt gccaagtatg acgagattat catgacgcaa tctgccgatc atatccatgt
     6781 tgatcatggc tgtagtctgc tccaaaggca cctttgggtt tttaatgtag taggatgaac
     6841 ccaagagacc gagctcttct ccagaaaacg cgataaatac cagattaaag ttaagtgaat
     6901 ctttattcga tgcgaaatat tcggcaattt ctattaatcc cgcagtgccg gaggcattat
     6961 cgtccgcacc gttgtgtatc tgcgtctgtc cttttttggt cttagcaaga gaacttactc
     7021 ctccatgccc aaggtgatcg tagtgggcgc ctattatgat tgtttttgtc tccggttttt
     7081 tatctgctgc ttcaataagg ccaatgacat tgtatgtatt gcttcggagc ctttgcagtt
     7141 cggtttgctg atgtactctg acttcgtaca ttgcaaaaga gtttggactg tgcgccgtta
     7201 tcttgttctc agtatctttt aggtcaagcc cgagaatact aaacaatttt tccaccgact
     7261 gtcttctaac aacagttgca tgaatatctg aggtagcgaa tgtaaaatca aacctgagac
     7321 ctccgaggtc tttttcttcc tcttttctgt agggtgtagt gaaaattata cctttggcgc
     7381 ctttttgctt tgctgtaatt gccttattgt atagtgaagt atatggactg tatcccttgt
     7441 cttttccaaa ctcttctgga gcataacgca gagcaataac gatcttatct tttacatcta
     7501 atccctcata gtcatcataa tttaaatcct tcgcagttat accgtaacct gcaaagacca
     7561 gatctgctgc tattttgccg tcattggaaa aactaagagg taaaaaatcc ga
//
LOCUS       DBNGSV010000189         6819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_657635, whole genome shotgun sequence.
ACCESSION   DBNGSV010000189 DBNGSV010000000
VERSION     DBNGSV010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_657635"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="AC159U_06705"
     CDS             complement(<1..2213)
                     /locus_tag="AC159U_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3336785.1"
                     /translation="MNKELVAILILLTTLVATNLFAQHSIDENKSSKSVSYNYNKDII
                     THTVVKGDSMGGLAYRYKTTVDYIKQLNNITRDQVFLGEVLKVPFNTKNSKGKDAYIS
                     YTIQKGDSLSQIASKYKTDTKSIKIVNKLGRNQINVGQELLIPYNKKFESSKAYDSKE
                     IIKTASKIDKKPAVIPVKGSKKKDYQKEIEDKYLSFLDKQERLKKEEQTKIKTASSTN
                     KSVKETSKSNINSPKDNKNYADLEKSLKNDLVKSTKREKVKTAKITKSENRESINKGA
                     YIDYVVKSGDSISILAQKHNTTAKTIRTLNNLERNQIDIGQTLKIPNIPASKYKTYRI
                     ESGDSLSYLADRHGITTKELMEINGLSSKQINIGQRIKLPLIEADVQEKPEIAAKEVK
                     VAKTETIDKKSEKNVEKTEIKPLKTTGGGPFQIASNKEEIILGVNTAKVKQQTTEKVE
                     NKTDLSNEKTAKSDKLPNKTDKLDNSKNNDKPVLVKAQTSKTDKVIKPDTKIEKEVKK
                     PETKPVIQVKKESIKTENTIKKVENEEKQSDIKPVIEAKKETKKDIIKPETTTQTKKE
                     EVIKVAESDSNTKKSFKVEKTQTGQSNIITYTVESGDSVSVLADKYNTTSADIRKLNS
                     LKDNNIKIGQKLKISKMPPSPYLRYKVQSGDSLSIVAEKHGLTTKQAMEINNLDSKQI
                     KIGQILKFPKEIDSKNEHKTTLVAMADSSKEAKIEEIISEPAKEVAVKKEIIEEK"
     gene            complement(2357..3289)
                     /gene="gspC"
                     /locus_tag="AC159U_06710"
     CDS             complement(2357..3289)
                     /gene="gspC"
                     /locus_tag="AC159U_06710"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HIR3336786.1"
                     /translation="MLSAIIKKYIKIVEFVLIALIAIVFVLIVHDNFVKPSLSTSNLK
                     NNNTNKNSTTRRAPTANSTRPRAYYKPILASNIFGLKNIAVSDSLAPGLTGDRDAPET
                     KLNMELIATIIDINNSQAVLKNLDNNKSNGYFIGDEVDIYKSEKIKISKVENCKVILE
                     RRSGYETIKCKTVTVASTSRKPTRNTRPEISKPDDHEDEALDEGIKKIGENEFEVDQE
                     LLDELLEDINEIMAEVRVIPNEEGLKLFGMRRNTFFYKIGLRNGDTLNMINDVELTDV
                     TNALGIFQELKGQPNFTINLTRRKKKHTFEYAVR"
     gene            3538..4032
                     /locus_tag="AC159U_06715"
     CDS             3538..4032
                     /locus_tag="AC159U_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIR3336787.1"
                     /translation="MNKKIKIYLAGPLGFSEVGRQFHSNIIIPAIKKLGFSYYDPWNT
                     RLNKQIKEVSSLEYGKRKRSAWYKLNLELGKLNTTEIESSDIILAVLDGVDVDSGTAA
                     EIGYAFAKGKKILGYRGDFRLSADNDGSTVNLQVEYFIRASGGTIITTIKQLNTELKK
                     LAAL"
     gene            4180..5118
                     /gene="bioB"
                     /locus_tag="AC159U_06720"
     CDS             4180..5118
                     /gene="bioB"
                     /locus_tag="AC159U_06720"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR3336788.1"
                     /translation="MTFEEAVALTELPKDEVPDLISLARKVTLKYKGDGVFLRSIISA
                     QTGNCPEDCSFCSQSVHFDTEVEAHPLMAAEKILGAARQAEKNGAMDFCIVISAKGPT
                     PRIFKKVLEAVDLLVKETELKVGCSLGDLTEEQAYTLRDHGVWRYNHNLETCRSHFPN
                     ICTTHTYDHRRKTAELVKETGMNLCCGGILGMGESVRQRLELAFELRELNPTWVPINF
                     LNPRPGTPFSEFEKVAPFEAVRTISIYRLILPDKIIMTAGGREVTLRDLQAMGLVAGA
                     NAMILGNYLTTPGRTPAEDLKMLDDLELPIRKDIAN"
     gene            5167..6522
                     /gene="bioA"
                     /locus_tag="AC159U_06725"
     CDS             5167..6522
                     /gene="bioA"
                     /locus_tag="AC159U_06725"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HIR3336789.1"
                     /translation="MGEDSKKTADLDKEYVWHPFTQMQEYEQKEPVVIEKGEGVYLYD
                     SEGKKYIDGVSSLWVNIHGHKVPEIDEAIKNQVDNISHSTLLGITNPPAAKLAEELIN
                     ICPEGLKKVFYSGDGASAVEVGIKMAFQYWKNKGKPEKTKFVYLENGYHGDTLGAVSV
                     GGIDMFHSAFKDLLFETYKAPSYYCYRCPLNKTYPSCNIACADEIDKILSEHHDEICA
                     VILEPYVQAAGGMIVAPEGYLKKVREYCDKHDVLLIVDEVATGFGRTGKMFACEHEDV
                     TPDILVLGKGMTGGYLPLSATITNQKVYDAFLGEYEEYKTFFHGHSYAGNPISCAASI
                     ANIEVFEKNNTLVELQEKIEYFENELKEFTGLSHVGNVRNKGLMAGIELVEDKKTKKP
                     YEAKEKMGWQVCDRAMDDEVLLRPLGNVVVLMPPLGIEMEDLKKLMKVTFQSIKETTE
                     P"
     gene            complement(6544..6684)
                     /locus_tag="AC159U_06730"
     CDS             complement(6544..6684)
                     /locus_tag="AC159U_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336790.1"
                     /translation="MKKGESPDKGESVLDGHSWIELNGEIFNDGDKDAPDIYQVTYSY
                     PN"
BASE COUNT         2002 a         1243 c         1206 g         2368 t
ORIGIN      
        1 ttcttctctt caattatttc tttttttaca gctacttctt tggccggttc tgatattatc
       61 tcttcgattt tggcttcttt actgctatca gccattgcaa ccaatgttgt tttatgctca
      121 tttttgctat ctatttcttt aggaaacttt aatatctggc caatcttgat ctgttttgaa
      181 tctagattat taatctccat agcctgtttt gtagtaagtc catgtttttc agccacaatt
      241 gaaaggctat ctccactttg cactttgtac ctgaggtacg ggcttggagg cattttagaa
      301 attttaagtt tttgtcctat ctttatatta ttatctttta aactatttaa cttccttata
      361 tcagccgatg tagtgttata tttatcagcc agcactgaaa cactgtctcc gctttcaact
      421 gtataagtaa taatgttact ttgcccggtt tgagtttttt cgactttaaa agatttttta
      481 gtattgctgt cagattcggc aactttgata acttcttctt ttttggtctg ggttgtggtt
      541 tcaggtttta ttatatcttt ttttgtttct tttttagcct caataactgg tttgatatca
      601 gactgttttt cttcattttc tactttttta attgtatttt ctgtcttgat tgattctttt
      661 ttaacctgaa taaccggctt agtttccggt ttttttactt ctttttctat ttttgtatct
      721 ggtttgataa ccttatcagt tttactggtt tgagctttta ccagcactgg cttatcgttg
      781 tttttagaat tatctagctt atctgtctta tttggcaatt tatctgattt tgctgttttc
      841 tcattcgata gatcagtttt attctctact ttttcagttg tttgctgttt tacttttgca
      901 gtattaacac ccaatattat ttcttcttta ttagaagcta tctgaaatgg tccaccacct
      961 gtagttttga gtggtttaat ttcagtcttt tctacatttt tttctgattt cttgtctatt
     1021 gtttctgttt tagctacttt aacttcttta gctgcaattt ctggtttttc ctgcacatcc
     1081 gcttcgatca aaggaagctt tattctttgg cctatattta tctgttttga actaagacca
     1141 tttatctcca taagctcctt tgtagtaatt ccgtgacgat cagctaaata agacaggcta
     1201 tcaccacttt ctattcgata ggttttatac ttgctggcag gaatatttgg aattttaaga
     1261 gtctgtccaa tatctatctg gtttctttcc agattgttta aagttcttat agtttttgca
     1321 gttgtattat gtttttgcgc aagtatggaa atgctgtcac cgctctttac aacatagtca
     1381 atataagcac ctttatttat ggattctcta ttttcagact tagtaatctt agctgttttt
     1441 actttttctc tttttgtgct ttttacaaga tcatttttaa gtgatttttc cagatcggca
     1501 tagtttttat tatctttagg actgtttata ttgctttttg aggtttcttt aacactctta
     1561 ttcgttgatg aagctgtttt tatcttagtt tgttcctctt tctttaatct ttcctgttta
     1621 tctaaaaaag atagatattt atcttcgatt tctttttgat aatctttctt tttactaccc
     1681 ttaaccggaa ttacagcagg ttttttatct attttagagg ctgtttttat gatttctttg
     1741 gaatcatagg ccttcgaact ctcaaatttc ttgttataag gaattaatag ttcctgccca
     1801 acgtttatct gatttctacc taatttatta acaattttta tagatttagt gtctgtttta
     1861 tatttagacg caatttgaga aaggctgtca cctttttgga ttgtatagct tatatatgcg
     1921 tctttaccct tggaattttt agtattaaaa ggaactttca gaacttcacc cagaaatacc
     1981 tgatcccttg ttatattatt taactgcttt atatagtcta cagttgtttt ataacgataa
     2041 gccagaccac ccatactatc gccttttaca accgtatgag ttataatatc cttattgtag
     2101 ttataactta cggatttact ggatttattc tcatcaattg agtgctgggc aaataaattg
     2161 gtagctacaa gtgttgtgag caatattaag attgccacta attctttatt catcttctcc
     2221 ctcatgaact tgctccaaaa tgtttaagaa attactttat gttttaatca taaactactt
     2281 ttaatattaa gtctagtact tttttatatt tataaaataa acaaaactct actggagttt
     2341 cattaaatgg tgtaaattat ctgactgcgt actcgaaagt gtgcttcttc tttcgccttg
     2401 taaggttaat ggtaaagttg ggctgacctt taagttcctg gaatattcca agagcatttg
     2461 tcacatctgt taattccaca tcatttatca tatttaatgt atcaccgttt ctaagtccta
     2521 ttttataaaa gaacgtatta cgcctcatac caaagagctt gagcccttct tcatttggta
     2581 tcacacgcac ctcagccata atttcgttaa tatcctctaa tagttcatcg agcagctcct
     2641 ggtctacttc aaactcattt tcaccgattt tctttatgcc ctcatcaaga gcttcatctt
     2701 catgatcgtc aggttttgat atttctggcc tggtgtttcg tgtaggcttt cttgatgttg
     2761 atgcaactgt tactgttttg cattttattg tttcatagcc gcttcttcgc tccaatatca
     2821 ccttacagtt ttcgacctta gaaatcttta ttttttcaga tttatatata tctacctcat
     2881 caccaataaa atagccgttt gatttgttgt tatccaaatt tttcaatact gcctgggaat
     2941 tgttaatatc tattatagtt gcaattaact ccatattgag ctttgtttcc ggagcgtctc
     3001 tgtcacctgt aagtcccggt gctaaactat ctgaaactgc aatattcttt aacccaaata
     3061 tattactggc aagaatgggc ttataatatg ctctgggtct tgtgctattt gctgttggtg
     3121 ctctgcgagt ggtgctattt ttattagtgt tattattttt aagattgctt gtagataatg
     3181 atggttttac aaagttgtca tgaactatta gcacaaaaac gattgcaatg agtgcaatta
     3241 gtacaaattc tacaatttta atatatttct ttattattgc cgaaagcatc ttaagtaatc
     3301 caattaataa ttaatttaaa aataaagtaa ttcacttaat atgtcaatag ttataaaaac
     3361 taattaataa tactatatat aataaatttc taaagaaaat taaagtcttt ttttaaagaa
     3421 ccccaataaa catcttctat gattagacaa tataaattaa ataaactaat attcattaaa
     3481 taagttgcta aaatagattg ctaataatga aaaaacaatc agacagtaaa attcacattg
     3541 aataaaaaaa tcaaaatata cctggccggt cctcttggct tttctgaagt gggtaggcag
     3601 tttcatagta acattattat tcctgctata aagaaactgg gattttcata ttacgaccca
     3661 tggaatactc gtctaaacaa acaaataaaa gaagtatctt ctttagaata tggcaaaaga
     3721 aaaagaagtg catggtataa gttaaatcta gaactgggaa agttaaatac cacagaaatt
     3781 gaaagttcgg atattatact tgccgtactt gatggagtag atgtggacag cggtactgcc
     3841 gctgagattg ggtatgcatt tgcaaagggc aagaagatat taggttatag gggagatttc
     3901 agattaagcg ccgacaatga cggatcgacc gtaaacctgc aggttgaata cttcattcga
     3961 gcaagcggcg gtacaattat tacaacaata aaacaactaa acacagagct taaaaaactg
     4021 gctgctttat aaattttata agcttgtgct tcaaattgac tttcatacct gtttttagta
     4081 aattagagtg ttcaaattta atctgctaat ttatttgggg ggcccaaaat cggagtctta
     4141 tcaactgtat cagaaaaatt attagaaaac gaagatcaaa tcacatttga agaagcggtg
     4201 gctttaaccg agcttccaaa ggatgaagta cctgacctta tttcacttgc aagaaaagtc
     4261 acactcaagt acaaaggtga cggagtattc ttaagatcca ttataagcgc acagaccggc
     4321 aactgccccg aggactgctc tttttgttca cagtccgtac attttgatac cgaagtagaa
     4381 gcccatcctc ttatggcagc agaaaagata cttggtgctg caagacaggc tgaaaagaat
     4441 ggcgccatgg atttttgcat cgtcattagc gccaagggtc ctactcccag aatattcaaa
     4501 aaggtactgg aagccgttga tcttcttgta aaagaaacag aacttaaagt tggctgctcc
     4561 cttggcgacc tcactgaaga gcaggcttat acactaaggg atcacggagt ttggagatat
     4621 aatcataatc tggaaacatg caggagccac tttcctaata tttgtacaac tcacacttat
     4681 gaccatagaa gaaaaaccgc agagcttgta aaagaaaccg gaatgaacct atgctgcgga
     4741 ggaatcttgg ggatgggaga atctgtaagg cagagacttg agcttgcgtt tgagctaaga
     4801 gagttaaacc ccacgtgggt gcctattaat ttcctgaacc cgcgcccggg cacaccattt
     4861 tcagaatttg aaaaggtagc accgtttgag gctgtaagga caatatctat ttatagactt
     4921 attcttccag acaaaataat tatgacagcc ggtgggagag aagttacact tagagattta
     4981 caggccatgg gacttgttgc cggagcaaac gccatgattc ttggcaacta tcttacaact
     5041 ccgggaagaa cacctgcaga agacctcaag atgctcgatg atcttgaact tccaatcaga
     5101 aaagacatag ctaactagtt ttttataaca tttctaccat agttcttaat ttataggcta
     5161 tccataatgg gtgaagattc aaaaaaaacc gccgatctgg acaaagaata cgtctggcat
     5221 ccttttaccc aaatgcagga gtatgaacaa aaagaacctg ttgttataga aaaaggagaa
     5281 ggcgtttacc tgtatgattc tgagggtaaa aagtacatag acggagtgtc ctcactatgg
     5341 gtaaacattc acggccacaa ggtacccgaa atcgatgagg ccataaaaaa tcaggtcgat
     5401 aatattagcc actcaaccct tcttggaata actaacccac ccgctgcaaa gcttgctgaa
     5461 gaattaataa atatatgccc cgaagggctt aaaaaagttt tctactcggg agacggcgca
     5521 agcgctgtag aggttgggat caagatggcc tttcagtact ggaagaataa aggcaagcca
     5581 gaaaagacca aatttgttta tctcgaaaac ggatatcatg gtgacacact cggagctgta
     5641 tctgttggtg gaattgacat gtttcattca gcgtttaaag accttctttt tgaaacatac
     5701 aaagccccgt catactactg ctacagatgc ccgcttaaca aaacttatcc ctcatgcaat
     5761 atagcctgtg ctgatgagat tgataaaata ctaagtgaac atcatgatga gatttgtgcc
     5821 gtaatcctcg agccatatgt acaggctgcg ggcggcatga tagtagcccc cgagggttat
     5881 cttaaaaagg ttagagaata ctgcgataaa catgatgtgc ttctaatagt agatgaagta
     5941 gcaaccggct ttggaagaac aggaaagatg tttgcctgtg agcatgaaga tgtgaccccg
     6001 gatattctgg tgctgggcaa gggcatgaca ggaggctacc tgccgctttc ggcaacaata
     6061 acaaaccaaa aagtctacga tgcattcctt ggagaatacg aagaatacaa gacattcttt
     6121 catggccaca gctacgccgg aaatccaatc tcgtgtgctg cttcaattgc caacattgaa
     6181 gtatttgaaa agaacaatac tcttgtcgaa ctgcaggaga aaattgaata ttttgaaaat
     6241 gagctaaaag aattcacggg gctttcccat gttggcaatg taagaaataa agggcttatg
     6301 gcaggcattg agcttgttga agacaaaaaa accaaaaagc cctatgaggc caaagaaaaa
     6361 atgggctggc aggtttgtga cagggctatg gacgatgaag tgcttcttcg cccgcttggg
     6421 aatgtagttg ttctaatgcc gccgcttgga atagagatgg aagacctaaa aaagcttatg
     6481 aaggttactt ttcagtcgat aaaggaaaca actgagcctt agtaattaat aatatagaaa
     6541 taatcagttc ggataactgt aagtcacctg atagatatct ggggcatctt tatccccgtc
     6601 attaaaaatc tcaccattta attcaatcca tgagtggcca tccagtactg attcgccttt
     6661 atcaggactc tcacccttct tcacaccata ataaataagg agctcgcata gaacagagct
     6721 ataagtaatg cagaatctcc tttattttat taactaaatc tacatttctg ttattaaatc
     6781 taaattccat ctcttttaga tacaaaacaa agtgttttc
//
LOCUS       DBNGSV010000190        11129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_310561, whole genome shotgun sequence.
ACCESSION   DBNGSV010000190 DBNGSV010000000
VERSION     DBNGSV010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_310561"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="AC159U_06735"
     CDS             <1..1076
                     /locus_tag="AC159U_06735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3336791.1"
                     /translation="IFEYEFLEALEQSGCVGEQTTWKPYYIILSDKEKDLIVGAVTFY
                     IRFDSYGEFIFDWEWARAFYDAGMNYYPKLLVAVPFTPTNGKRIHVDPDYSFETCAAI
                     MVEYLIEICREKNFSSIHFLFLTNKEQEFLESYGFLSRTTHQYHWKNRDYNTFDDFLA
                     DLRAGRRKQIKKERKYLAESDLNIDVIDKNNVMPHHIEAIWQFYIDTSSRKWGTPYLN
                     KKFFELFYEKMRDRIVLVMAKNGNGWAGGTFNVVKNQSLYGRYWGCLEDFRYLHFECC
                     YYSLIEYAISNNIEIFEAGAQGEHKFLRGFGAYPTYSSHYINNEGASAAISGFLDSEK
                     IYISNLIDRYNNKSPLKYLHNKS"
     gene            1103..1441
                     /gene="clpS"
                     /locus_tag="AC159U_06740"
     CDS             1103..1441
                     /gene="clpS"
                     /locus_tag="AC159U_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR3336792.1"
                     /translation="MSGSKIAKNDREGKVLTQERVRLKKPDMYKIVLLNDDYTPREFV
                     VWVLIKVFYKSEHESLRIMLDAHTKGKSMIGVYTHDVARTKVMQVDKLAEQNGHPLKC
                     DLEVEQGGEC"
     gene            1438..3684
                     /gene="clpA"
                     /locus_tag="AC159U_06745"
     CDS             1438..3684
                     /gene="clpA"
                     /locus_tag="AC159U_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR3336793.1"
                     /translation="MTLSKELEDTLQRAYDLAKAKKHEFITLEHILFEMTNESGASEV
                     LMSCGVDLDKLKYDLAEFMDKSMPSIMSEDLPEPQYSVGSQYVLRIAAMHVQSAGKEE
                     LKSGNLIVAMFREEESHAVYFLKEQGVDRLSVVKYVSHGGAPSSEQETKTTNKNPDGE
                     PVPQVKNPLTEYCTNLVEKAKAGKLDPLIGRDKELERTVHILCRRRKNNPIFVGDAGV
                     GKTAIAEGLANRIVEDRVPEVLKDLQIYSLDMGALTAGTRYRGDFEERLKAIIDSVKG
                     NRNNVLFVDEIHTVIGAGAVSSSGLDASNMLKPALSNGEIRCIGTTTLKEYRSVFEKD
                     HALARRFQKIDVHEPSNEDTLKILNGLKGYYEDFHKVTYSAPALKAAVDLSDRYINDK
                     KLPDKAIDLLDEAGAEVKLRNYKSEIKQVSVKDIEALVSRIAKIPSRTVKVDDRNRLM
                     TLSADLKKVLYGQDQAIEAIVKSIQMSRAGLNEPDKPVGSFMFSGPTGVGKTELAKQL
                     AAAMGIEFLRFDMSEYMEKHTVSRLIGSPPGYVGYDQGGQLTEAVNQNPHAVLLLDEI
                     EKAHEDIYNILLQVMDHATLTDSNGRKVDFKQIILILTTNTGSRESSQHTMGFGKQEY
                     EDKSKETIDKYFSPEFRNRLNAIVQFNSLSKEVVEQVVDKIVGQLSERLSSKKITIEL
                     SPEARSYIAESAYDPLLGARPIQRFIDSEITEKLTNEILFGKLLNGGSVKILLENGEL
                     KIETLIEN"
     gene            complement(3671..4333)
                     /locus_tag="AC159U_06750"
     CDS             complement(3671..4333)
                     /locus_tag="AC159U_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336794.1"
                     /translation="MSEIQDILISKHEKVPKTDDPIALKTSWEPTNPGGANFKTQQLT
                     VTKNRIIIEKTYGTIVLSLIFILIGIPSLIYGSRYYYSKGMVWEGVFAFVIGIIFTIC
                     GILVLKFFKKTTFDLIAGVYYRGKKYDKFNSVDRSIQGLLSDIYAIQLIYEILRSGST
                     KVSNTYKSYEMNLVFKDGERLNIMDHGKQEDIDAAAIKLGEFLQVPVWKASYSNTNSN
                     SQ"
     gene            complement(4364..4768)
                     /locus_tag="AC159U_06755"
     CDS             complement(4364..4768)
                     /locus_tag="AC159U_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336795.1"
                     /translation="MNPEQVESAINTALQQDQSSTDKIKVYISNLQITDRGRPSDVKY
                     YFYKNQLYKLKIKQDLATHKKPIINQLIKLKGLDEQFNDIVQSLHYGRKYETFNDNQK
                     IVFYLNSGDLNSSDTVFLDLKYLPVAGQTNFE"
     gene            complement(4967..6151)
                     /gene="nhaA"
                     /locus_tag="AC159U_06760"
     CDS             complement(4967..6151)
                     /gene="nhaA"
                     /locus_tag="AC159U_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR3336796.1"
                     /translation="MLKNKNKFIRTFFKMESAGGILLMISSLLAIIFANTPLLTYYEL
                     LLSTPVEVAVGSLQIAKPLLLWINDGLMAIFFFLVGLELKRELTEGELSDKRSIILPG
                     VGAVGGMLAPALVYLYFNYSDPVALKGWAIPAATDIAFALGVLALLGSRVPISIKIFL
                     TSLAIFDDLGAILIIAFFYTSKISLTALIIVAICIPILIVLNKRNVISNSPYILIGII
                     MWIAMLKSGVHATLAGVLMAMFIPMRSKTDPEFSPLKTIEHDLHSIVAFFVLPVFAFA
                     NAGISFSDIESHQILHGVPLGIAFGLFFGKQIGIFGLCWIFIKLRITSLPAGMNWKSL
                     YGTAALCGIGFTMSLFISSLAFEETGVNLLFDERIGIILGSLTSGILGYAVLRKSLSS
                     NK"
     gene            complement(6461..6844)
                     /locus_tag="AC159U_06765"
     CDS             complement(6461..6844)
                     /locus_tag="AC159U_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3336797.1"
                     /translation="MSFPIYAGQDTKGFKVDVVLNSTATMSGHPLSVPSGDNIKIEAH
                     VVQIAPKSEIGKHSHPYPVLTYVISGSMEVENEDGSKQTFSAGQSFIEDANTWVINRN
                     NSNEPVKFLAVLYAQKDQQTVMLKE"
     gene            6975..7253
                     /locus_tag="AC159U_06770"
     CDS             6975..7253
                     /locus_tag="AC159U_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HIR3336798.1"
                     /translation="MSHKYKIQIEYCTQCRWLIRAAWIAQELLTTFEAELGEVSLIPG
                     TGGIFQIRSENKILWDRKNQDGFFDIKELKRIVRDHVAPDKNLGHTDK"
     gene            7234..7368
                     /locus_tag="AC159U_06775"
     CDS             7234..7368
                     /locus_tag="AC159U_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336799.1"
                     /translation="MVTLTNNETLYSMLDLYGNGKDLYELYMSKHGGDGGPGGTIKLK
                     "
     gene            7431..8006
                     /locus_tag="AC159U_06780"
     CDS             7431..8006
                     /locus_tag="AC159U_06780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3336800.1"
                     /translation="MKNIRSGVIVLGFFLSTALHAGSADLNPFKLKDPLIGKKAPSLE
                     TEVWFNSSAISLDEIRGNVVIIEFFQLWCPGCNKFSIPLMNRWRDETFSSESDVKFIS
                     VHTVFEGHEYQDNERLKYFIKEKEITHPVAVDKHIEGRHTPVTMAKYRTGGTPCIAII
                     DKKGIVRFKYFGGFKTEPVEGFIKELLKETS"
     gene            complement(8065..10224)
                     /locus_tag="AC159U_06785"
     CDS             complement(8065..10224)
                     /locus_tag="AC159U_06785"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3336801.1"
                     /translation="MQEYKNKTMKQIKKDLPVEVREELDEFDKKVRDYEMGLIGEIKF
                     QKIRLQLGVYAQRQEGYQMQRIKIPYGGLNSNQLRRLADVADKYANNFLHLTTRQDVQ
                     IYYLNIKQVPNMMRELADVGITTREACGNTVRNVSACHISGVSPTESFDVTPYAEAFK
                     NFLLRNPICQNMGRKFKVCFEGCHDTDHAGIRIHDIGFQARIKEVDRKQRRGFEIWVG
                     GGLGASPSLGHLWTEFMPVEEMIPFSAAIIRIFDRHGERKVRMKARMKFLIRKLGMDE
                     FRKLVEAEMAQLKIDPSWNDYLNDIPGQETAPEFSHDIPAAPEGTEDNPEYKHWLATN
                     VVPHRTDDFKMVHIRVKLGDISAENARRLAEITEVFSDSTIRISILQNFMLRWVPTSA
                     LPSLYNALKQLDLAAAGANGFEDITACPGADTCRLGITSAKGLSNKLNKSMTNGLGKY
                     ADVTKDLKIKISGCPNSCAQHVVANIGFQGAAISNQGRSVPAEMVFLGGALKGENTTL
                     AMPIGKVPTRNAPKLVERLLEIYKSEKNEGEDFDDVMRRLGAKELKPVLKEYGDIPSY
                     EDDPDFYKDWGHEHEKFEVQQGVKGECAGATVEEKVPTLGEAQYHIEQAEALLSHGEF
                     EASMNEAFLACSASAHVPLYTKLVDPFTPEQTLWEFENLLVRTGEADSKWLNIAERLA
                     DERSQEAEEEQAVEMLDIAKEFYSECEYVQEQLTAGS"
     gene            10445..>11129
                     /gene="mtgA"
                     /locus_tag="AC159U_06790"
     CDS             10445..>11129
                     /gene="mtgA"
                     /locus_tag="AC159U_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020288611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR3336802.1"
                     /translation="MKNLIKKLKKIVKWSVLLFFASTFVWTAVYRYINPPFTPLMLAR
                     YIQDDRDEKVIEKSWINLDHISQNTILAVVAAEDQSYFSHWGFDFNQINKSILDYEKK
                     GSFRGASTISQQTAKNVFLLPVRSWLRKGFESYFTVLIEILWGKKRILEVYLNVAELG
                     SGYYGVEAASNHYFGKSASKLNQREAAHLASLLPNPREYHKKVYSDYRKKKTTWIIKQ
                     MKNLGGTGYI"
BASE COUNT         3417 a         2079 c         2271 g         3362 t
ORIGIN      
        1 cgattttcga atatgaattc ctagaggcac tagagcagtc tggctgcgtg ggagagcaaa
       61 ccacgtggaa gccttattat attattctca gtgataagga aaaagattta atagtaggag
      121 cggttacctt ttatatcagg tttgattcct acggggagtt tatttttgac tgggaatggg
      181 ctagggcatt ctatgatgca ggaatgaact actaccctaa actccttgta gcagtcccct
      241 ttactccaac aaacggtaaa aggatacatg ttgatcctga ttattcattt gagacctgtg
      301 ccgcaattat ggttgagtat ctaatagaaa tctgcaggga aaagaatttt tcttccatac
      361 attttttgtt tctcaccaat aaagagcagg agtttttgga gagttacggc tttctttcaa
      421 gaaccaccca tcaatatcac tggaagaaca gggattataa tacatttgat gattttctgg
      481 ctgaccttag ggcaggcagg agaaagcaga taaaaaagga gagaaaatat ttggcagagt
      541 cggatctaaa tattgatgtt attgataaaa ataacgttat gcctcatcat atagaagcaa
      601 tctggcagtt ctatattgat acatcgtctc gtaaatgggg tactccttat ctaaataaaa
      661 agttttttga attattctat gaaaagatga gagacaggat tgtgcttgtg atggccaaaa
      721 atggtaatgg ctgggctggg ggcactttta atgtggtcaa gaatcagagt ctttatggaa
      781 ggtactgggg gtgtctggaa gattttagat atcttcattt tgagtgttgt tactacagtt
      841 tgattgagta tgctatttct aataatatcg agatatttga agcaggagca cagggtgagc
      901 acaaattctt aagaggtttt ggggcttatc ctacatatag ctctcattac attaacaacg
      961 agggcgcaag tgctgctata tcaggctttt tagatagtga aaaaatttat ataagtaatt
     1021 taattgatag atataataac aaatcaccgc taaaatattt acataataaa tcttaagatt
     1081 tatcatttat actataattc agatgagcgg aagcaagata gcaaaaaacg acagggaagg
     1141 aaaggtttta acgcaggagc gtgtgaggct taaaaagcct gatatgtaca agatcgtcct
     1201 gcttaacgat gattacacac caagggagtt tgtggtctgg gtgcttataa aagtatttta
     1261 taaatcggaa catgagagtt taaggataat gctcgatgcg cataccaagg ggaagagtat
     1321 gattggtgtt tatactcacg atgtagcaag gacaaaagtg atgcaggttg ataaacttgc
     1381 agagcaaaac gggcatccgc taaaatgtga tctggaagta gaacaaggag gtgaatgttg
     1441 actctatcaa aagaactcga agacacgctg caaagggcat atgaccttgc aaaggcaaag
     1501 aagcacgagt ttattacgct tgagcatata ctgtttgaaa tgacaaatga atccggagct
     1561 tcggaggtct taatgtcatg cggggttgat ctggataagc tcaaatatga tcttgccgag
     1621 tttatggaca agagcatgcc ctctataatg tcagaggatc tgccagaacc gcagtattcg
     1681 gttggcagcc agtatgtact caggattgcc gcaatgcatg tgcagtctgc cggaaaagaa
     1741 gagcttaaga gcggaaatct catagtagca atgttcaggg aggaagagtc acacgctgtt
     1801 tactttttaa aagagcaggg ggtggacagg ctttcagtag taaaatatgt gtcacacggt
     1861 ggtgccccaa gctcagagca ggaaacaaaa acaacaaaca agaatccaga tggcgaacct
     1921 gttccacagg ttaaaaatcc gcttacagag tattgtacta atcttgtaga aaaggctaaa
     1981 gctggaaagt tagatccact cattggaagg gataaagaac tggaaagaac cgttcatata
     2041 ctctgcagga gaagaaaaaa taaccccata ttcgttggtg atgcaggggt tggtaaaacc
     2101 gcaatagccg aggggcttgc aaacaggatt gtagaggatc gggtgccgga agttttaaaa
     2161 gacctgcaga tttattcact cgatatggga gctctaacag cgggaacaag atatagagga
     2221 gattttgaag aacgactaaa ggcaataata gattcagtta aaggaaatcg gaataacgtt
     2281 ttatttgtag acgaaataca cacagtaata ggtgctggtg cggtatcaag ctccgggctt
     2341 gatgcatcca atatgctaaa gcctgcgcta tcaaacggcg agataaggtg catcggaaca
     2401 actacattaa aagagtacag aagcgtattt gaaaaggacc atgcgctagc aagaaggttt
     2461 cagaagattg atgtacatga gccaagcaat gaagatacgc ttaagatact aaatggtctt
     2521 aagggctact atgaagattt tcataaagtg acttattcag ccccagcgct taaggccgca
     2581 gttgatctgt ctgacagata cattaatgac aagaaacttc ccgataaggc aattgatctc
     2641 ttagacgaag ctggtgcaga ggttaagctt agaaactata aatctgaaat taaacaggtt
     2701 tctgtaaaag acattgaagc gcttgtttca agaatagcaa aaattccttc cagaactgtg
     2761 aaggtagacg acaggaacag gcttatgacc ctttctgccg accttaaaaa agttctttac
     2821 ggccaggatc aggcaattga ggcaattgtt aaatcaatcc aaatgtccag ggcaggacta
     2881 aatgagccgg ataaaccagt aggttctttt atgttttcag gccccacggg ggtaggtaaa
     2941 actgagcttg caaaacagct tgcagcagca atgggtatag agttcctgcg gtttgatatg
     3001 agtgagtaca tggaaaagca cacggtgtcg agactcattg gctctccccc tggatacgtg
     3061 ggatatgatc agggtggcca gcttactgaa gcggttaatc aaaatcctca cgcagtattg
     3121 cttcttgacg aaatagaaaa ggcacacgaa gatatctata acatactgct acaggttatg
     3181 gatcatgcca cgctcacaga ctccaatgga aggaaagtag actttaagca aatcatactt
     3241 attcttacta caaacacagg gtcaagagag agcagccagc acacaatggg atttggtaaa
     3301 caggaatatg aagataagag caaggaaaca attgataaat atttcagccc tgagtttaga
     3361 aacagattaa acgcaatagt gcagttcaat tcactgagta aagaagttgt tgaacaggta
     3421 gtggataaaa ttgtaggaca gctttctgag agactaagct caaagaaaat tacaattgag
     3481 ctatccccgg aggccagatc ttatattgcc gagagtgcat atgatccact tcttggcgca
     3541 aggccaattc aaagattcat cgattctgag atcacagaaa aactaacaaa tgagatactt
     3601 tttggaaagc tgttgaatgg tgggtctgtg aaaatactgc ttgaaaatgg tgagcttaag
     3661 attgaaacac tcattgagaa ttagagtttg tattggagta gcttgctttc cagactggaa
     3721 cttgcaaaaa ctctcctaac tttattgcag cggcatctat atcctcttgc tttccatgat
     3781 ccataatatt tagacgttct ccgtctttaa acacgaggtt catctcatag cttttgtagg
     3841 tattacttac ttttgtagaa ccggaacgta atatttcata tattaattgt atagcgtaaa
     3901 tatcagaaag caggccctga atagaccgat caactgaatt gaatttatcg tattttttac
     3961 ctctataata aacgcctgca attaaatcaa aggttgtttt cttgaaaaat tttaggacta
     4021 atattccaca aatggtaaat attataccta taacaaaagc aaatacgcct tcccaaacca
     4081 tacctttgct ataataatat ctagagccat aaataagtga aggaattcca attaatataa
     4141 aaattaatga tagaacaata gtgccgtaag ttttttctat aataattcga ttttttgtaa
     4201 cagtgagttg ctgtgtctta aaattagccc cgcctggatt tgttggctcc cagctagtct
     4261 ttaatgctat ggggtcatcg gttttgggga ctttctcatg tttgcttatc agtatatcct
     4321 gaatttcgct cattattaca gtctactatg gcctaagatt atatcattcg aaatttgttt
     4381 gaccggcaac aggcaaatat ttcaaatcta aaaaaacagt atcggagcta tttaaatcgc
     4441 ctgagtttag atagaaaact attttttgat tatcattgaa agtctcatat ttccttccgt
     4501 aatgaagtga ttggacaata tcattaaact gctcgtccag tccttttagt tttattagct
     4561 gatttatgat cggtttttta tgagtagcta aatcttgttt tatctttagc ttgtataact
     4621 gatttttata gaaataatat ttaacatcgg agggcctgcc tctgtcagta atttgcagat
     4681 tacttatgta aacttttatt ttatctgtgc tggattgatc ttgttgtaga gcagtattta
     4741 ttgcactttc aacctgctcc ggattcatgc cccagtgcat gtttagtatg gcggtgttgg
     4801 tgaaggaata tgaagaattg atttcatata aaaatataga aaataataaa agaacaatcc
     4861 cacagaattt cattattaat atattaaata cttagataaa caaataaagt attattggaa
     4921 ataatattag agatctgctg ctagactcta tatctgttgg atgagtttat ttgttggaac
     4981 tcaaactctt cctaagaact gcatatccta aaatacccga agtcaacgag cctaaaataa
     5041 tgccaattct ctcatcgaat agtaagttga cgccggtttc ttcaaatgcc agggaactaa
     5101 taaataaact cattgtaaat cctataccgc agagagcagc agtaccgtag aggctcttcc
     5161 agttcatgcc agcgggcaga cttgttattc ttagttttat aaaaatccag caaagtccga
     5221 atattccgat ctgctttcca aaaaatagtc caaatgctat tcctagcggc acaccgtgca
     5281 gtatctgatg agactctata tcactaaaac tgatacccgc atttgcaaat gcaaacacag
     5341 gcagtacaaa aaaggctaca atagaatgca gatcatgctc gatggtttta agtggagaaa
     5401 attcgggatc agtcttcgat ctcatgggaa taaacattgc catcaagacc ccggcaagcg
     5461 tggcatgaac tcctgattta agcattgcaa tccacataat aattcctatt aaaatatatg
     5521 ggctgttgga tatcacattg cgtttattaa gcacaatcaa tatcggtatg caaatggcga
     5581 caataataag ggcagtaagc gagatttttg atgtatagaa aaaagctatt atcagtattg
     5641 ctccaagatc atcaaatatt gcaagtgaag tcaaaaatat tttaatagaa attggtactc
     5701 ttgagccaag taaagctaat acgccgagtg caaatgctat gtctgtggca gccggtatgg
     5761 cccagccctt gagtgccact ggatcgctgt agttaaaata tagatagaca agtgcggggg
     5821 caagcatacc accgacagct cccacaccgg gaagtatgat acttctttta tctgataatt
     5881 ctccctcagt taattctctt tttagttcca gtcctacaag aaaaaagaat atggccatta
     5941 gcccatcgtt tatccacagt agcagaggtt ttgcaatttg tagtgaacca actgcaacct
     6001 ctacaggagt cgatagcagc aattcatagt aggtaagcag tggggtgttt gcaaatataa
     6061 tggctaaaag cgatgagatc atcagcagta tcccgcctgc agattccatc ttaaaaaatg
     6121 ttcttataaa tttattttta tttttaagca tttgaaaaaa taattacgat caagagttca
     6181 attacataca aaatatatct tcatcagtat agcctgaaaa tttataggtg tagatattag
     6241 ctttataata aaaaagccga gctgatctcg gctttttgga gggtttcttt ctaaaccaaa
     6301 agaaacagtc gcttttcttg cattccctta tacctgcccc atgcaaacct tactacatct
     6361 atattatact attttttata ttaaaatgat agcaataata ttgctatggg tagatattga
     6421 ttaatataca aaaacgggat attaaaaatt cagcaactcg ttattctttt agcataacgg
     6481 tttgctgatc tttttgggca tagagaactg caaggaactt gaccggctca ttactgttgt
     6541 tcctgtttat aacccatgta ttcgcatcct ctataaatga ctgacctgcg ctaaaagtct
     6601 gcttggatcc gtcttcattt tccacttcca tactgccgct tattacatat gtcagcaccg
     6661 gatacgggtg gctgtgtttt ccgatctcag attttggcgc aatttgtacc acatgggcct
     6721 caattttgat attatccccg cttggaacac tcagaggatg gcctgacatt gttgctgtgg
     6781 aattgagaac aacatctacc ttgaaacctt ttgtgtcttg cccggcatat attggaaaag
     6841 acataatcga tatcaataac ccacaaaata ttatgtgttt cataatacac ccccataatg
     6901 atatgatcta ttttaattgt ataagtttct tgtataaaat taaagcataa aaatacttaa
     6961 tttaaaaaat taatatgtct cataaataca aaatccagat cgaatactgc actcagtgca
     7021 ggtggctaat acgggcagcc tggattgcac aggaactgct tactaccttt gaggcagaat
     7081 tgggcgaagt ttcgcttatt ccgggcacgg ggggtatttt tcaaatccgc tctgaaaata
     7141 aaatcctgtg ggatagaaaa aatcaggacg ggtttttcga tataaaagag ttaaaacgga
     7201 ttgtgcggga tcatgttgct ccggataaaa atcttggtca cactgacaaa taatgaaact
     7261 ttatactcca tgctcgatct ttatggaaat ggaaaggatc tctatgaact ctatatgtca
     7321 aagcatggcg gagacggagg cccgggtggt acgattaaat tgaagtagga tttatgaacc
     7381 caattttaat ttgtagaatc ttatattcta aatattaaaa tggtgatgat atgaagaata
     7441 taagatcagg tgtgatagtt ttggggtttt ttctgagcac tgctttgcat gcgggttcgg
     7501 ctgatttaaa tccctttaag ttaaaagacc cgctaattgg caaaaaagct ccgtccttag
     7561 aaactgaagt ctggtttaac agctcggcaa taagtctcga tgagattagg ggtaatgtgg
     7621 ttattataga attctttcag ctctggtgtc cgggctgtaa taagttttct attcctctta
     7681 tgaacaggtg gagggatgaa acattcagct cggagtcaga tgtgaaattt ataagcgtac
     7741 acactgtgtt tgaggggcat gaatatcagg ataatgaaag gctgaaatat tttataaagg
     7801 aaaaagaaat tactcatccg gttgctgttg ataaacacat tgagggcagg catacccctg
     7861 ttacgatggc caaatacaga accggcggta ccccgtgtat tgcaattata gataaaaaag
     7921 gaattgtcag attcaaatac tttggcggtt ttaaaactga gcctgttgaa ggctttataa
     7981 aagaacttct caaagaaact tcataaagaa aagccccgcc gaatattctg acgaggctct
     8041 gtattctata atatttttta ttagttaact tccagccgtc agctgttcct gaacatattc
     8101 acattcggaa tagaactctt ttgcaatgtc cagcatttct acagcctgtt cttcttcagc
     8161 ttcctgagac ctttcatctg caagtctctc ggcaatgtta agccatttgc tgtctgcttc
     8221 accggttctt acaagaagat tttcaaattc ccacagggtc tgctccggag tgaacggatc
     8281 aacaagtttt gtgtatagcg gcacatgggc gctagctgag catgcaagga atgcctcatt
     8341 catactagcc tcaaactctc cgtgtgatag aagagcctct gcctgctcaa tatgatactg
     8401 tgcctcaccg agcgttggaa ctttttcttc taccgtagct ccggcacact cgccttttac
     8461 tccctgctgt acctcaaact tttcgtgctc atgaccccag tctttataaa agtcgggatc
     8521 gtcttcgtat gaaggaatat cgccgtactc ctttaacact ggttttaatt ctttggctcc
     8581 gagccttctc attacatcat caaaatcttc accttcgttc ttctcacttt tgtaaatttc
     8641 taaaagtctt tctacaagtt taggagcgtt tctagttgga acctttccta taggcatggc
     8701 aagcgtggtg ttttctccct ttagtgctcc gccaagaaaa accatctctg ccggcactga
     8761 tctgccctga ttggaaattg ctgcaccctg aaaaccaata tttgcgacta catgctgtgc
     8821 gcacgagtta gggcagcctg agatttttat tttcagatct tttgtaacat cggcatattt
     8881 cccaagaccg tttgtcatgc ttttatttag tttattagat agcccttttg cagaggtaat
     8941 tcccagtctg catgtgtcgg ctccggggca tgctgtgatg tcttcgaaac cgtttgctcc
     9001 agctgctgca aggtctaatt gttttagtgc attataaaga gacggcaggg ctgatgtcgg
     9061 aacccacctg agcatgaaat tctgcaggat ggaaattctt atggtgctgt ctgaaaaaac
     9121 ctcggtaatc tcagcaagcc ttcttgcatt ctcagcagat atatctccaa gcttaactct
     9181 tatatgtacc atcttaaaat cgtctgttct atgtgggaca acatttgtgg caagccagtg
     9241 tttgtattcc gggttgtctt cagtaccttc tggagctgca ggtatatcat gactgaattc
     9301 aggtgcggtc tcctgcccgg gaatatcatt taaataatca ttccatgatg ggtcaatctt
     9361 aagctgcgcc atttctgctt caaccagctt tctaaattca tccataccca gtttgcggat
     9421 taaaaacttc atcctggctt tcattcttac ttttctctca ccatgacggt caaaaattct
     9481 tattattgct gctgaaaatg gaatcatttc ctcaacaggc ataaactctg tccacaggtg
     9541 cccaagcgat ggcgaagccc ccaggccgcc gccaacccaa atctcaaaac ctcttctttg
     9601 ctttctgtct acctctttta ttcttgcctg aaaaccaata tcatgaattc ttattcccgc
     9661 gtggtctgtg tcatgacaac cctcaaaaca tactttgaat ttcctgccca tgttctgaca
     9721 gattgggttt cttagtaaaa agtttttaaa tgcttctgcg tatggagtaa catcaaatga
     9781 ctctgttgga gatactcctg atatatggca agcagatacg tttcttacag tgtttccgca
     9841 agcttctctg gttgtgattc ccacatcggc aagctctctc atcatgtttg gaacctgctt
     9901 tatgtttaaa taatagatct gtacgtcctg ccttgttgtg aggtgcagga agttgttagc
     9961 atatttgtca gctacatcgg caagtcttct gagctgattg gagttaagcc cgccataggg
    10021 aatcttgatt ctctgcatct gatacccttc ctgcctctgg gcatatacac ccagctggag
    10081 tctaatcttc tgaaacttga tctctccaat aagccccatc tcataatcac ggactttttt
    10141 atcaaattcg tccagctctt ctcttacttc tacaggcaga tcctttttga tctgcttcat
    10201 tgttttattc ttatattcct gcattatttt gtctccaact aatgttcagt gattaattat
    10261 atcaaattaa cggtctattt gaacttatta tcgttattct gcacgctaaa tattgaacta
    10321 tagtgttatg caattattat accgtacaat atctcattat ttagctagat tatgcataat
    10381 tattgactaa tatttataca ttctgattat agtttaatca atatttatga gttctgtttg
    10441 ataaatgaaa aatctgatta agaaattaaa gaagattgta aaatggtcag ttttattatt
    10501 ctttgcatcc acatttgttt ggacggctgt ttatagatat atcaatcctc cttttactcc
    10561 tttaatgctt gcaagatata ttcaggatga tagggatgaa aaagtcatag aaaaaagctg
    10621 gataaatctg gatcatatat cccagaacac catacttgct gtcgttgcag cagaggatca
    10681 gagctatttc agtcactggg ggtttgattt taaccagatc aataaatcta tattagacta
    10741 tgaaaaaaag ggaagcttca ggggggcaag caccataagc cagcagactg caaaaaatgt
    10801 gtttcttctt cctgtacgca gctggcttcg taagggattt gaatcttact ttacggttct
    10861 tattgaaatt ttatggggta aaaaaagaat tcttgaggtc tatttaaacg tagcagaatt
    10921 gggtagcggt tattacgggg tagaagccgc atcgaatcat tatttcggca aatcagcaag
    10981 caagctaaac cagagagaag cggcacacct ggcgtctctt cttcctaatc ccagggagta
    11041 tcataaaaaa gtttactcgg attacaggaa aaagaagacc acctggatta ttaagcagat
    11101 gaaaaatctg ggtgggacag ggtatattg
//
LOCUS       DBNGSV010000191         9024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1904683, whole genome shotgun sequence.
ACCESSION   DBNGSV010000191 DBNGSV010000000
VERSION     DBNGSV010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1904683"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="AC159U_06795"
     CDS             <1..950
                     /locus_tag="AC159U_06795"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3336803.1"
                     /translation="KNLITLANNHSETVGLDESEKILFTLPISHTYALGNFISAIKSG
                     MTCYLLEDFNRKKVTEIIIKQQITIYPCVPFILDILAESNIDPDTFKSLKLVISAGAA
                     LSKEIFDKFSNKFAKHPRQLYGSSETGVISINLSSNIENTSDSVGRPVKGVTVKIVTD
                     EGKEADTGELGEIIVKSETMTNGYDNMPQETKEVFKDGFYYTGDLGYMDNDGMIYIKG
                     RKKLMINISGNKVDPVEIENILTSHPKIHESAVVGITDQNSTEIVKAFIVCDENIERK
                     DIIQYLMNQVSDYKIPKLIEFVQSIPKSPTGKILRNKLK"
     gene            954..1205
                     /locus_tag="AC159U_06800"
     CDS             954..1205
                     /locus_tag="AC159U_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3336804.1"
                     /translation="MSEELKLKIKEIIINKFNLGIDVVDFDEQKPFIEYGVGIDSVAN
                     LELILQIEKNLKINIDEAQVNSEILFNFKTLYKFLRDQI"
     gene            complement(1209..1937)
                     /locus_tag="AC159U_06805"
     CDS             complement(1209..1937)
                     /locus_tag="AC159U_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3336805.1"
                     /translation="MTDLSMSDLIEFSKVKNVNLGADPEKTIKSCVKLGLIPKPKRRK
                     VKDDKGRTTTLFFPDYTVDKLAYIKALKSEGLSLEEIRDNFAHEFVKDAINGLLKDAD
                     EDKIKHLANIIGSDTRQLEAIVEAPLVNVMEVMSAEEVKRILCLFFGVGFYSLLEAQK
                     ALEEFRINDAKKAISKSTFYNSVGVLRLARTAGDTKLEETASEVYDSVVIKPIAKATE
                     MIRIQFLDSLSSYLETKNTKENAK"
     gene            2091..2702
                     /locus_tag="AC159U_06810"
     CDS             2091..2702
                     /locus_tag="AC159U_06810"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3336806.1"
                     /translation="MDILKDHRIVAVIRAENIDEAMLKADACISGGIKAIEITFSFDQ
                     CCRAINKIKENSDIVVGAGTVLTVDNAKRAVEHGADFIVSPHTCKNIIEFTKKENKVV
                     ISGASTSNEIVTAHSIGADMVKIFPANLIGGPGYVKAIKEPLPFAKIFVTGGINLQNC
                     SDYITSGASLIGVASALFTHNSQTPGKKITENAQKFVEKVHNI"
     gene            2744..4213
                     /locus_tag="AC159U_06815"
     CDS             2744..4213
                     /locus_tag="AC159U_06815"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR3336807.1"
                     /translation="MNILFITSEFDPLTNIGGLGEVTSSLSKELQGLGCNVKIVLPYY
                     KAVKSNLQNLKIKPKSINKTITTCIDWLAVSAKVKEVEVNGITCYLIDNKDYFDRNYI
                     YSAYGYELGDDDLRFGFFSLCALQLAKSLNFQPNIIHCHDWQTAFVPLFLKYRKHLKD
                     DPFYKSSKVLFTIHNLYYQGEFREELLETFGLPDYLFTNQNIELKGKVNLLKAAILFS
                     DQITTVSETYAKEIINPEFGFGLDRVLRRASQDSNKMHGILNGIDYNLHNPNYDKEIY
                     KNYGLDSLENKQYNKTKLKEEFGLDTSEDKPLIAVISKLTDQRGIQLIIDSFSQIIDL
                     GYQLFILGVGDSKYHKALSHKTKKYKGNYCLILKQDDHTAAKIYAGADMILMPSKFEP
                     CGLGQMIALRYGTIPVVRSTGGLVDTVRDYSESNKPTGFTFKQFTKVNLLDTLIKAIT
                     VYEHKNEWEKIIKNAMKEDYSWKVSAKKYLDLYEKTIKN"
     gene            complement(4230..4532)
                     /locus_tag="AC159U_06820"
     CDS             complement(4230..4532)
                     /locus_tag="AC159U_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR3336808.1"
                     /translation="MIKHIVMWKLKDSARGVSKEENAKELKSVLEDLKDKIDVIKEIE
                     VGMDFNGSAAAYDVALYSVFDSREDLQTYQKHPEHVKVVDFVNEIRDERVVVDYEI"
     gene            complement(4627..5613)
                     /locus_tag="AC159U_06825"
     CDS             complement(4627..5613)
                     /locus_tag="AC159U_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3336809.1"
                     /translation="MKIILVTDAWEPQVNGVVYTLKNVVNNITDHEVFVLHPSVEGSV
                     TLFNLYYNIPILKNPFEVVRNYFGEQKPDRVHIATEGSLGLSARYYCKKYGIKFNTSY
                     HTQLADYGWLLYKVPRFITNLFIRWFHSASGRVLVTTKSIEKQLNFKNSVVWARGVDA
                     SLFNNYSRSANVRGNTLIFVGRVSKDKNLDDFCSIEGYKKILVGDGPYLSTLKKHYPD
                     VEYTGFIDHKELVNWYNKAGVFVFPSKFDTYGLVMLEAMACGLPVVAYDVPSPCDVVE
                     NGVTGYLGDNLTKSIEKVFADYTNLSNNAQKFAKSKSWKSISDQFVSHLVNI"
     gene            complement(5826..6266)
                     /locus_tag="AC159U_06830"
     CDS             complement(5826..6266)
                     /locus_tag="AC159U_06830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3336810.1"
                     /translation="MNIDFKSIVLVAIQLVCIVFILATGRLIPHSLPLQIVYVLAIAL
                     GLWALWTINFKNFRLTPNFPKDARIVAKGPYKLVRHPMYSCLLLVTLMLVIDDFSIIR
                     FAVWLILLADLYVKLVYEEKLIIYRNSDYQIYKRGTKRLIPFIH"
     gene            complement(6304..6696)
                     /locus_tag="AC159U_06835"
     CDS             complement(6304..6696)
                     /locus_tag="AC159U_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336811.1"
                     /translation="MRETGDFVIVRVNNRPVLIFYSSNDKNKQWYLPGLDLLTNINRQ
                     IAIETEILGNSRPENDDRNNMIDSGEFVILSKSRFKTIFKLEGKNINQKFIMLTPSWG
                     RWTQRPISLIIKLNEPDNNFLTGKQLCL"
     gene            complement(6680..7444)
                     /locus_tag="AC159U_06840"
     CDS             complement(6680..7444)
                     /locus_tag="AC159U_06840"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3336812.1"
                     /translation="MLLAVDIGNTNIVFGLIDSKGIHHSWRIGTDKTKTQDEYGVLLR
                     NLFEYKKVNISGLCGSVISCVVPALQDILVECIHDYFDIEPVVVGPGVKTGMQILYKN
                     PQEVGADRIVNAIGAFEEYKRSLVIVDFGTATTFDCVSKKGEYLGGVITPGITISSEA
                     LFGSASKLPKVEIKKPENVIGKTTIQSIQSGLVYGYAGLVDGIVGRIKKELGAGTVVI
                     GTGGLAGLIAKESETIEKIDEHLALKGLWIIYERNR"
     gene            7621..8919
                     /gene="miaB"
                     /locus_tag="AC159U_06845"
     CDS             7621..8919
                     /gene="miaB"
                     /locus_tag="AC159U_06845"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR3336813.1"
                     /translation="MDEKYLYIETYGCQMNDYDSQRIKNAIGIPQTDDPGKADIVIIN
                     TCAIREKADQKALSSLGKFKHLKRANPNLVVGMTGCVAQLYGEKLLSKASHLDFVLGP
                     RAIPKLAEIIKDVEIERTRIVETSYDVEEPFEILPYHENEKVTSFLSIQQGCNKNCTY
                     CIVPTVRGKEINRPVEDIMSEAKYLVGKGVKEITLIGQTVNSWKNDGLKFADLLNIVG
                     ELESLERIRFTTSYPRDFTKKLINTMKTNPKVCSHIHLPVQSGSNEVLSKMKRTYTVE
                     WYLDVVNRLKEEINDMAISSDIIVGFPGETEHDFNQTLKLVEQVGFDTCFSFKYSPRP
                     ATPAAVMENSVDSDTSGRRLTILQELQRKITCKKNEDRIGRVEKVLVEGKSKNDLNHL
                     MGRTSQNRIVNFSSNEDLSGKLVNVKINRGFQNSLLGSIL"
BASE COUNT         3087 a         1624 c         1528 g         2785 t
ORIGIN      
        1 ataaaaatct gattactctt gctaacaacc acagtgaaac cgtggggttg gatgaatcgg
       61 aaaaaatatt gtttaccctg ccaatttctc atacatatgc ccttggcaac ttcatttctg
      121 caataaaatc cggtatgacc tgttatttac ttgaagattt taacaggaaa aaagtaacag
      181 agattattat taaacagcag ataacaattt atccttgcgt tccctttata ctagatatcc
      241 tggctgaatc aaatattgat cctgacacat ttaagagtct taagcttgta atatccgcag
      301 gcgctgcgct ttctaaagag atatttgata agttttccaa taaatttgca aaacatccaa
      361 gacagctcta cggatcttca gaaacagggg ttatttctat aaatctaagc agcaatatag
      421 aaaacacatc cgattctgtg ggaagacccg taaaaggggt tacagttaaa atagtaaccg
      481 atgaaggcaa ggaagcagat accggagaat tgggtgaaat aattgtaaag agtgaaacaa
      541 tgactaacgg atatgataat atgcctcagg aaaccaaaga ggtttttaaa gatgggtttt
      601 attatacagg cgatcttggc tatatggaca atgacggaat gatctatatt aagggtcgca
      661 agaagcttat gataaatata agcggtaata aagtcgatcc tgttgaaatt gaaaatattt
      721 taacttcaca tccgaaaata catgaatccg ctgtagtagg aattacggat caaaacagca
      781 ccgaaatagt aaaggctttt attgtttgtg atgaaaatat tgaaaggaaa gatataatcc
      841 aatatctaat gaatcaggtt tcagattata aaataccaaa actaatagaa tttgttcagt
      901 ccattccaaa aagccctact ggaaaaatcc taaggaacaa actcaaataa acaatgagtg
      961 aagaattaaa actaaaaatt aaagaaataa taataaacaa attcaatctt ggcattgatg
     1021 tcgtcgattt cgatgaacaa aagcccttta tagagtatgg agtaggaatt gattctgttg
     1081 ctaatttaga acttatactc caaattgaaa agaaccttaa gatcaatata gatgaagcgc
     1141 aggttaatag tgaaatactg tttaatttta aaactcttta taaatttcta agagatcaga
     1201 tatgacaact atttagcatt ctctttggta tttttggtct ctagataact tgaaagtgaa
     1261 tccagaaact gtatccgtat catttctgtg gcctttgcaa taggtttaat gaccacgctg
     1321 tcataaactt ctgaagcagt ttcttctaat ttggtatcac ctgctgtcct tgccagtctc
     1381 agtactccta cagagttata aaacgtagat ttggatatcg ccttctttgc atcattaatt
     1441 ctgaattctt ctaaagcttt ttgcgcctcg agaagagaat aaaatccgac tccgaagaac
     1501 aggcatagta ttctctttac ctcttcagca gacattacct ccattacatt taccagtgga
     1561 gcttccacta ttgcttcaag ctgcctggta tcactaccta taatatttgc aagatgcttt
     1621 atcttatctt catctgcatc tttcagaaga ccatttatgg cgtctttaac aaattcatgg
     1681 gcaaagttat ctcttatctc ttcaagcgac aggccctccg atttaagagc tttaatgtac
     1741 gcaagcttat ccacggtgta atcggggaaa aacagggttg tagtcctgcc cttatcgtcc
     1801 ttcacctttc ttcttttagg ctttggtata agacctagtt ttacgcagga ctttatagtc
     1861 ttttcaggat cagctccaag attaacattt ttaacttttg aaaattcaat cagatcggac
     1921 atagaaagat cggtcatatt ccgatattaa ggtattttgt atgtttttgt caatacagta
     1981 atttgttttt atattaaatt agtaaaatag ttttaatact gtattttctt aaagcaaaat
     2041 ttgctaatga taacggcagg ttaaaatttg aaggggtttg gcagcgttta atggacattt
     2101 taaaagatca tagaatagta gccgtgatta gagccgaaaa tatagatgag gcaatgctta
     2161 aggcagatgc ctgtatttcg ggtggaataa aagcgataga aatcactttt tcctttgatc
     2221 agtgctgcag ggcaattaac aagataaaag aaaatagcga tattgttgta ggagcgggga
     2281 cagtgcttac tgtggataat gctaagcgcg cagtagaaca cggtgctgat tttatagttt
     2341 ctccccatac ctgtaaaaat attatagaat ttacgaaaaa agagaataag gtagtaatat
     2401 cgggagcttc tacatcaaat gaaatagtta ctgctcatag tataggtgca gatatggtaa
     2461 aaatatttcc tgccaattta attggcggcc caggatatgt aaaagctatc aaggaacctc
     2521 ttccatttgc aaagatattt gttacaggtg gtataaatct gcaaaattgc agtgattaca
     2581 taacctccgg agcatcttta ataggagttg caagcgcatt atttactcac aactcacaaa
     2641 caccgggaaa aaagataact gaaaatgctc agaaatttgt tgaaaaagta cataatattt
     2701 gatacaagat agataatact tctaaaacta atatacccaa ctcatgaata ttctttttat
     2761 tacatcggag ttcgatccac ttactaatat aggaggtctt ggtgaagtta catcgtcact
     2821 ttccaaagaa ctgcagggtc ttggatgcaa tgtaaagata gttctgcctt attacaaagc
     2881 agtaaaatct aatctgcaaa atctaaaaat taagccaaaa tctattaaca aaacaattac
     2941 tacctgtatt gactggcttg ctgtatcggc aaaagttaaa gaggttgaag taaatggtat
     3001 cacttgttat ttgattgata ataaagatta ttttgataga aactacatat acagcgcata
     3061 tggatacgag ctgggagatg atgacctcag atttggattt ttctcacttt gtgcactgca
     3121 gcttgcaaaa tctctaaatt ttcagccaaa cataatccac tgccatgact ggcagacagc
     3181 ttttgttcct ttgttcctaa agtaccgcaa acatttaaaa gatgatccgt tctataaatc
     3241 atccaaggtc ttattcacaa ttcacaacct ttactatcag ggggagttca gagaggagct
     3301 actggaaact tttggtcttc cagactactt gtttactaac caaaacatag aattaaaagg
     3361 caaggtcaat ctgctaaaag cagcaatact tttttcagat cagattacaa ctgttagtga
     3421 aacttatgca aaggaaataa taaatcccga gtttggattt ggactggaca gggttcttag
     3481 aagagcttcc caggactcca ataaaatgca tgggatctta aatgggatag attacaacct
     3541 tcacaatccc aattatgata aagagattta taagaattat ggtttggact cattggaaaa
     3601 taaacaatac aacaagacta agctcaaaga agaatttggt cttgatacca gtgaggataa
     3661 accattaatt gccgttatat ccaagcttac agatcaaaga ggcatacagt taataattga
     3721 ctcattttca cagattatag acctgggata tcagctattt attcttggag tgggtgattc
     3781 aaaatatcat aaagcgctct cacataaaac aaagaaatat aaggggaatt actgtctgat
     3841 actaaagcag gatgatcata cagcagcaaa gatctatgca ggagccgaca tgatattgat
     3901 gccttcgaag tttgaaccct gtggacttgg gcaaatgatt gcgcttagat atggaactat
     3961 cccagttgta agatccaccg gaggtttagt ggatacagta cgtgattata gtgaaagcaa
     4021 caagccaaca ggttttacat ttaagcagtt cacaaaggta aacctgctgg ataccctcat
     4081 aaaggcaatc acagtttatg agcacaaaaa tgaatgggaa aagattatta agaacgccat
     4141 gaaagaagat tattcatgga aggtatcagc caaaaaatat ttagatcttt atgaaaaaac
     4201 aataaagaat taaattctat gattctaaat cagatttcat aatctactac tactctttca
     4261 tcccttatct catttacaaa atcaaccact tttacatgct ccgggtgctt ttgataggtc
     4321 tgcagatcct ctcttgagtc aaataccgaa tacaatgcaa catcatatgc cgcagccgat
     4381 ccgttaaaat ccatacctac ttcaatttct tttataacat ctattttatc tttcaggtct
     4441 tccaatacag atttaagctc cttagcattt tcttctttac taactccacg agcactatct
     4501 ttaagtttcc acattactat atgtttaatc atttaagcct ccatcggttt ttaagtattc
     4561 aaaagttaac tgaatattaa taattatcta attccttact gattttgaaa caccacatat
     4621 ttaaattcat atatttacca ggtgggaaac aaactgatct gaaatagatt tccagctctt
     4681 tgattttgca aacttctgtg cattgttcga aaggttagta taatcagcaa aaactttttc
     4741 tatactcttg gtcaaattat cccccaagta tcctgttacc ccgttttcaa ctacatcaca
     4801 ggggcttgga acatcatatg ccacaacggg aagcccgcag gccatagctt cgagcatcac
     4861 cagaccatat gtatcaaact tactcgggaa cacaaataca cctgctttat tataccaatt
     4921 tacaagctct ttatgatcta tgaaaccagt atactcaaca tcaggataat gcttcttaag
     4981 tgtgcttaag tatggcccat ctcctaccaa tattttttta tatccttcaa tcgaacaaaa
     5041 atcatccagg tttttatcct tgctcactct tcctacaaaa attaatgtat ttcctcttac
     5101 attggcagat ctgctgtaat tattaaacag tgaagcatca actcctcttg cccaaaccac
     5161 cgaatttttg aagtttaact gtttttctat actctttgtg gtaaccaaaa ccctgccact
     5221 agccgaatga aaccatctta taaataaatt tgtaataaac ctgggcactt tatacaaaag
     5281 ccagccatag tctgcaagct gtgtgtgata agaagtatta aactttatcc catacttctt
     5341 acagtaatac cttgctgaaa gacccaggct tccctctgta gcaatatgaa ctctatcggg
     5401 cttttgctct ccaaaatagt tccttacgac ctcaaaaggg ttttttagta ttggaatatt
     5461 ataataaaga ttaaaaagtg tcacagagcc ttcaacgctg ggatgaagta caaagacttc
     5521 atgatcagta atattattaa ccacattttt aagtgtatag acaacaccat ttacctgagg
     5581 ttcccaggca tcggttacaa gaattatttt cataaaagtt tctttgagat tggatattat
     5641 aaataattaa gcccaatgta catagtcatt aattctctac tttaggagat agaaattttg
     5701 ctatgtttct ggatttattt ttagttagta aatgatcgtt attctttata tggaatgtgc
     5761 atcaattata taagtaaaac taaaaattaa tatacatcaa ttttgtgtac aatagtttta
     5821 gcaaattaat ggatgaaagg aatgagtctt ttggttcccc ttttgtaaat ctgataatct
     5881 gagtttctat atattattaa tttttcttca taaacgagtt ttacatataa atcagctaat
     5941 aaaatcagcc aaacagcaaa tcttataata gaaaaatcat caataacgag cattaaagtt
     6001 acaagcaata ggcatgagta catgggatgc cttactaact tatatggtcc tttggcaact
     6061 attcttgcat cttttggaaa atttggagta agacggaaat ttttaaaatt tattgtccag
     6121 agcgcccaca aacctagtgc aatagcaagc acatatacta tttgaagtgg taagctgtga
     6181 ggtattaatc ttcctgtagc caaaataaat acaatacata caagctgtat tgcaacaaga
     6241 actatcgatt taaagtctat attcataaat aaattagata ggaatatgat tgtacaggta
     6301 ttatcataga cataattgct taccagtcaa aaaattattg tcaggttcat taagtttaat
     6361 aataagtgaa ataggtcttt gagtccacct gccccagctt ggtgtaagca taataaactt
     6421 ttgattgata ttttttcctt caagtttgaa tatggtttta aatctggatt tactaagtat
     6481 aacaaattca cctgaatcaa tcatattatt tctatcatca ttttcaggac gagaattgcc
     6541 taagatctcg gtttctatag ctatttgcct gttaatgtta gttagtaaat caaggcctgg
     6601 taagtaccac tgtttatttt tatcatttga tgaataaaat atcagaaccg gtctattgtt
     6661 caccctaact atcacaaaat caccggtttc tctcatatat tatccaaagc cctttcaatg
     6721 ctaaatgttc atctattttt tcaattgtct cgctttcttt agcaataagc ccggcaagac
     6781 cgcctgttcc aatcacaact gtaccagcgc caagctcttt ttttattctt cccactattc
     6841 catctacaag cccggcataa ccatagacaa gaccggattg aatgctctgg atagtggttt
     6901 taccaataac attttcaggt ttttttattt ctacctttgg cagtttggat gcagatccaa
     6961 atagcgcttc agaggaaatc gtaatcccgg gagttatcac tccacccaga tattcacctt
     7021 ttttagaaac gcagtcaaaa gttgtagcag taccaaaatc tacaatcaca agacttctct
     7081 tatactcctc aaatgcaccg attgcattta caatcctatc agcaccaact tcttgaggat
     7141 ttttatacaa tatttgcatt cccgttttta ctcccgggcc tacaacaacc ggttctatat
     7201 cgaaataatc gtgaatacac tctactagga tatcctgaag tgctgggaca acacatgaga
     7261 ttacagaacc gcaaagtcct gatatattaa ccttcttata ttcaaaaaga tttcttaaca
     7321 gaactccgta ttcatcctgc gtcttagtct tatcagtccc tattctccag gaatgatgta
     7381 tgccctttga atcgatcaat ccaaagacta tattcgtatt tcctatgtct actgctaata
     7441 acatggctac ctatcctaaa ataaaatcat ggcagagttg atatacatat aatgatttat
     7501 taaaacaatc ttaatcatag cagtaaactt tgtaaatcaa aaggtatagc ataaataaat
     7561 tgtgcaatat tttactgatc cttgacttaa aaccaagcaa tcgttaaggt ttagattcaa
     7621 atggatgaaa aatacctata tatagaaact tatggctgtc agatgaatga ttacgattca
     7681 cagagaatca aaaatgctat aggtattcct caaacggacg atcccggtaa ggctgatatt
     7741 gttatcatta atacttgcgc tataagagaa aaagcagacc aaaaggcgct cagcagtctt
     7801 ggaaaattta aacatcttaa aagagcaaac ccaaatttag ttgtaggtat gacagggtgt
     7861 gtagcacagc tttatggtga aaagcttctc agtaaagctt cgcacttaga ttttgttctc
     7921 ggtccgcgcg caatacccaa gcttgcagaa ataataaaag acgtagaaat tgaaagaaca
     7981 agaatagtgg agacttccta cgatgttgaa gagccttttg aaattcttcc atatcacgaa
     8041 aatgaaaagg tcacttcatt tttatccatc cagcagggat gcaataaaaa ttgtacttac
     8101 tgcatagttc ctacggtaag aggaaaagaa attaacagac cggttgagga tataatgtcc
     8161 gaagctaagt atcttgtagg taaaggtgtc aaggaaatca cacttatagg acagactgtt
     8221 aactcatgga aaaatgacgg tctcaaattt gcagacctgc taaatatcgt tggcgagttg
     8281 gaatcactag aaaggataag atttacaaca tcctatccaa gagattttac aaaaaaacta
     8341 atcaatacaa tgaagaccaa tcctaaagtg tgcagccata ttcaccttcc cgtgcagtcc
     8401 ggatccaacg aagtattgtc caagatgaaa agaacttata cagtggagtg gtacctggat
     8461 gtagtaaaca gactaaaaga ggaaattaat gatatggcaa tttcgtctga cataatagta
     8521 ggttttccag gagaaaccga acacgatttt aatcaaacgt tgaaacttgt tgaacaagta
     8581 ggatttgata cttgtttttc atttaaatat tcacccagac cagcaactcc ggctgctgtt
     8641 atggaaaatt ccgtagacag tgatacttct ggcagaagac tcacaattct tcaggaatta
     8701 cagagaaaaa tcacatgtaa aaagaatgaa gatagaatag gtagagttga aaaagtgctt
     8761 gtcgaaggaa aaagcaagaa cgatttaaac catttaatgg gaagaacctc tcaaaacaga
     8821 atagtaaatt tcagctcaaa cgaagatctg tccggaaaat tagttaatgt aaaaataaat
     8881 agaggctttc aaaattcctt attaggctct atactataat aggactagtg ctgtggggag
     8941 aacagtatgt tgtatgaaat gaaagttagt ggaattgcta tcgatccgtt tacaaacact
     9001 ccaatcatta tacttaaaga ctta
//
LOCUS       DBNGSV010000192         5321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1419278, whole genome shotgun sequence.
ACCESSION   DBNGSV010000192 DBNGSV010000000
VERSION     DBNGSV010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1419278"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..378
                     /locus_tag="AC159U_06850"
     CDS             178..378
                     /locus_tag="AC159U_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336814.1"
                     /translation="MNIKNYNNLLTQYDIDKFCDDWSTKIFHFPDDDNELKKEEGWVN
                     TFTRVMECEEEWEERKKQNLSE"
     gene            590..1306
                     /locus_tag="AC159U_06855"
     CDS             590..1306
                     /locus_tag="AC159U_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3336815.1"
                     /translation="MKLKDKNVLITGGSKGIGKGLAKVFLEQGANVIICARNKDVLDD
                     TVNELSAVGNIRGFSVDITKLSDVKDLQASVSNEFKHLDVLLNNASILGVRAKIKDTP
                     EDIWDSVIKINLNSQFYVIKAFLPLLEKSTNASIINVSSSVGRKGKAEWGAYAASKFG
                     LEGLTQVLADELKDTNIRVNSVNPGGTRTDMRADAYPDEDPLTLPTPEDISPIFLHLA
                     SDDSIDTNGQAFNARDYLDN"
     gene            complement(1312..1980)
                     /locus_tag="AC159U_06860"
     CDS             complement(1312..1980)
                     /locus_tag="AC159U_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3336816.1"
                     /translation="MSDSRESILNTISNSLRKKPESTFEPYSGFDNNDYLTINYNTDL
                     LTEFSQKLESLSGNVKVVKNKRNLSKSVTDIFSSTNSNNFIGWECVELSSVYRRLEKL
                     GYKHIKSNRKSQQAKCDIGITLADYAISESGTIVLYNNKTQNRNSSLIVPIHIAILRK
                     EKIVKSIFDLIARLSNDYSTLENINELSNCITLITGPSRTADIELNLTLGVHGPKELY
                     VVVM"
     gene            complement(1973..3364)
                     /locus_tag="AC159U_06865"
     CDS             complement(1973..3364)
                     /locus_tag="AC159U_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3336817.1"
                     /translation="MYYKYNFEKESAKALDNQNLQEALTNTTNRFRTARSNNVLESNN
                     WEQLRQVAHNIKEHTINNIHNYLILLENNIIQAGGQVHWAKDSEEACTIIRDIAINNN
                     VKKIVKSKSMATEEIHLNKSLQSNGYKVIETDLGEYILQISDDKPSHIIAPAIHKRKV
                     DISKLFSEKFGVPEYDDPQELTKIAREKLRQQFLTADMGISGVNLAAADTGTIAIVEN
                     EGNARLTTTVPKVHVALMGIEKIIPRFDQMGLFLKLLIGSATGQKISTYVSFITGPRK
                     RGESDGPKEFHLVLLDNGRSKMLTNPNTRESLYCLRCGACLNICPVYRQVGGHAYGGT
                     YSGPIGAIINPQLEGLDKYPDLPFASSLCGACKDVCPVKIDFPKVLLDLRRQVVENKK
                     NSRAGGLEKSVFIIWKKMNQHPRLLKLLNNIAHYMPHSLISKIFSRWTKSRDITHIPQ
                     KTFKQIWKDEKNV"
     gene            complement(3379..4116)
                     /locus_tag="AC159U_06870"
     CDS             complement(3379..4116)
                     /locus_tag="AC159U_06870"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3336818.1"
                     /translation="MSFNNNVSLFLTCLVDNMYPNVGTSMVKVLRRAGAHNINIPENQ
                     TCCGQPAYNSGYQSEAKEIAIKFLDDFKDSEYIVCPSGSCVSMIKVFYKDLFKNDDNY
                     SDIANVISHNTYELSDFIINILGVSNIGAMYRGKVTYHDSCHALRELGISGQPRELLK
                     NVDGLQLIEMDMHDACCGFGGTFSVKYPDVSTAMLKEKVQCIIDSGADTIVSTDMGCL
                     MNIQGYISRNNISIKVHHIAEILAITD"
     gene            4242..>5321
                     /locus_tag="AC159U_06875"
     CDS             4242..>5321
                     /locus_tag="AC159U_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3336819.1"
                     /translation="MQNDFYKELFDNSNDLIQSVSPQGDILYVNKTWLNKLGYKRSEI
                     KDLNIFQIIHPTSINHCTNLFQKIMLQETSISIDEFKLLTKNGDSIILEGYASSKFEN
                     NKPLYTRGIFKDITDKVKTEKNLEILNLVTQNLNETNDLRQAYKIALDKVCELDYVDI
                     ACIYIVDNITREAVLEDHRNLPDDYLKRASRISFPKGITWKTILSGNILNVKDIQKVK
                     VLGKAGKKLGIRSFVGIPIKIDSKSIGVFWLLSIKKHLFSKNEVSLLNSIGNQIAISI
                     ARSKSYDELIKKNKYDEIISSVVKSVHSSIDLQQVFENTVDAIFNNIKTIKHVAVQIV
                     EGNSANIKAYRGYPKWFINEVRKIER"
BASE COUNT         1794 a          834 c          856 g         1837 t
ORIGIN      
        1 ggtttattac ctaaaattct aacttgtaat ctggtactat tatagggggg aaggtcaaat
       61 ataagaaggc gtttatatct aacaacagat aagcttaatt tttatgaaag aactggaaag
      121 aaggaatatt taaatttttt aaaagattat tataagtatg gggaaattaa agcatttatg
      181 aatataaaga attataacaa tttacttact cagtatgata ttgataaatt ttgtgatgat
      241 tggtctacta aaatatttca tttcccagat gacgataatg aattaaagaa agaagaaggg
      301 tgggttaata catttactag agtaatggaa tgtgaggaag aatgggaaga aagaaaaaaa
      361 caaaatctat cggaataagt aggatatcat tagtaataag gggtgattat acaaacccaa
      421 attattatga tctaaaacca tccaatattt atctaatgaa ccaaatcctt aatatttata
      481 cataatagta tctatatata atagttaaca taatatatct tatcggacaa taactctatt
      541 tctttccata atagcaatat aaccgtcttt acgctataat acgcccatta tgaagctaaa
      601 agacaagaat gtgttaatta caggtggttc taaaggtatt ggtaagggtt tagctaaggt
      661 ttttttagaa caaggcgcaa atgttattat ttgtgccaga aataaagatg tcttagatga
      721 tacagttaat gaactaagcg ctgtaggtaa tataagagga ttttcggtag acattacaaa
      781 actttcggat gttaaggatt tacaagcttc agtttcaaat gaatttaaac atcttgatgt
      841 actattaaat aatgcaagca tactgggggt aagagcaaaa attaaggata ctcccgaaga
      901 tatatgggat tctgtaatta agattaatct aaattcacag ttttatgtga taaaggcttt
      961 tcttccatta ttagaaaaat ctactaatgc atcaataata aacgttagct ccagtgtcgg
     1021 tagaaaagga aaagctgaat ggggagccta tgcagcatcc aagtttggac ttgagggtct
     1081 cacacaggtc ttggctgatg aactgaagga tacgaatatc agagtaaact cagtcaatcc
     1141 cggcggtaca agaacagata tgcgagctga tgcttaccct gatgaagatc cattaactct
     1201 accaacgcca gaggatatat ctcccatttt tcttcaccta gcttctgacg actccataga
     1261 tacaaatgga caggcattca atgcaaggga ttatttggat aattaatagg tttacataac
     1321 aactacatac aactcttttg gtccatgaac gccaagggtt aagttaagct caatatctgc
     1381 agttctgcta gggcctgtta ttaaggtaat acagtttgaa agttcattta tattttccaa
     1441 tgtactgtaa tcattagata atctggctat cagatcaaat atgctcttta ctatcttttc
     1501 tttccttaag atagctatat gtattggtac aataagggat gaattcctgt tttgtgtttt
     1561 attattgtat aaaactatag tgcctgattc agagatagca taatctgcaa gagtgatacc
     1621 tatatcacat tttgcttgtt gagattttct attactcttg atatgtttgt accctaattt
     1681 ctctaacctt ctgtatacac tagataattc aacacattcc caaccaataa agttgtttga
     1741 gttagttgaa gaaaaaatat cagtgactga tttactaaga ttcctcttgt ttttaactac
     1801 ttttacattt ccgcttaatg attctaattt ttggctgaat tctgttagaa gatctgtatt
     1861 ataattaatc gtaagataat cattattatc aaaaccacta tatggttcaa aagtactctc
     1921 aggttttttc ctcaatgagt tactgatagt atttaaaata gattctcttg aatcagacat
     1981 ttttttcatc cttccaaatc tgtttaaatg ttttttgtgg aatatgggtt atgtctctgg
     2041 atttagtcca tcttgaaaat attttagata ttaaagaatg aggcatataa tgtgcaatat
     2101 tattcaataa tttcagtaat ctgggatgct gattcatttt cttccatatt atgaatactg
     2161 atttttctaa accccctgcc ctgctattct ttttattttc aacaacctgt cttcttaaat
     2221 ccaataaaac ctttggaaaa tcaattttta ctggacacac atctttacag gctccgcaaa
     2281 gagaagatgc aaaaggaaga tcagggtatt tatccagtcc ctcaagctgt ggatttataa
     2341 tagcgccaat tgggcctgaa tatgttccac catatgcatg ccccccaacc tgcctgtaaa
     2401 caggacatat attaaggcaa gccccgcatc ttaggcagta aagcgactcc ctggtgtttg
     2461 gatttgtaag catcttgctc cttccattat cgagcagaac caggtggaat tctttggggc
     2521 cgtcagattc cccccttttt ctaggacctg tgataaatga tacataagtg gatatctttt
     2581 gccctgtggc actacctatc agaagtttta gaaataagcc catttgatca aatctgggta
     2641 ttatcttttc tatacccata agagccacat gcactttggg cactgtggta gtaagccttg
     2701 cgttaccctc attttctact atggcaatag ttcctgtgtc tgctgctgcc aggtttactc
     2761 ctgatattcc catatcagca gttaagaatt gctgtcttag cttttctcgt gcaattttag
     2821 tcagttcttg cggatcatca tactctggga ctccaaattt ttcagaaaat aactttgata
     2881 tatcaacttt ccttttatga attgctggag caatgatatg tgaaggttta tcgtctgaaa
     2941 tttgtaaaat atactcacca agatcagtct caattacctt gtaaccatta gactgcagtg
     3001 atttattcag atgaatctct tcagtagcca tggacttact tttaactatc tttttaacat
     3061 tattatttat agctatatct cttataatag tgcaggcttc ttcagaatct tttgcccaat
     3121 gaacctgtcc accagcttgt ataatattat tttctaataa tatcagatag ttatgtatgt
     3181 tgtttatagt atgttcttta atattatgtg caacttgccg taattgttcc caattattag
     3241 attctaaaac gttatttgaa cgtgcagtcc ggaatctatt ggttgtattt gtaagtgctt
     3301 cctgtaggtt ttgattatct aatgctttag ctgattcttt ttcaaagtta tatttatagt
     3361 acataatatt taatattatc agtcagttat agctaatatc tcagctatat gatgaacttt
     3421 tatagatata ttatttctgc ttatataacc ctgaatattc ataagacatc ccatatctgt
     3481 tgaaacaata gtgtctgctc cggaatctat gatacattgc accttttctt tgagcattgc
     3541 tgttgataca tctggatatt taactgaaaa cgttccacca aatccacagc atgcatcatg
     3601 catgtccatt tctatcagtt gtaaaccatc aacatttttt agaagctctc ttggctgacc
     3661 acttattcca agttctctta gtgcatggca ggaatcatga taagttactt tacctctata
     3721 cattgctcca atattactaa ctcctaatat attaataata aagtcggaga gctcatatgt
     3781 gttatgagaa ataacatttg caatatcaga gtaattatcg tcatttttaa aaagatcttt
     3841 ataaaatact tttatcatag atacacacga acctgaggga cagactatgt attcggaatc
     3901 tttaaagtca tctagaaatt taatcgctat ttcttttgct tcagattgat atccgctatt
     3961 atatgcaggc tggccgcagc atgtctgatt ttctggaatg ttaatattat gtgcccctgc
     4021 ccttcttagt accttaacca tagatgttcc aacattagga tacatattgt ccacaagaca
     4081 agttaaaaat agagatacat tattattgaa tgacattata tttagtggat aagttttaat
     4141 tattaaagaa aataatgctt aatattaatc ttaaacaggt atataattaa taactcatta
     4201 atgaatataa cataacttag ttgattctca tttattaacc tatgcaaaac gacttttata
     4261 aagaattatt cgataactct aatgatctta ttcaaagtgt atctcctcaa ggtgatattc
     4321 tgtatgttaa caaaacctgg ttaaataaat tagggtataa aaggtctgag attaaagact
     4381 taaatatatt tcaaattata catccaacct ccattaatca ttgtactaat ttattccaga
     4441 aaataatgtt acaggaaaca tcgatatcaa ttgatgaatt taagcttctt acgaaaaatg
     4501 gagattcaat aatacttgaa ggatatgcaa gttctaaatt tgagaataat aaacctttat
     4561 atacaagagg tatttttaaa gatatcacag ataaagtaaa gacagagaaa aatctagaaa
     4621 tattaaatct tgtaactcaa aatttaaatg aaaccaatga tcttaggcag gcatataaaa
     4681 ttgctcttga taaagtctgt gaactggatt atgtggatat agcatgtatt tatattgttg
     4741 acaatattac acgagaagca gttcttgaag atcatagaaa tcttccagat gactatttaa
     4801 aaagagcttc tcgtatatcc ttccctaaag gtattacatg gaaaacaata ctatctggca
     4861 atattcttaa tgtaaaagat atacagaaag taaaagtatt aggaaaagca gggaagaaac
     4921 ttggaattcg tagttttgta ggaataccta ttaagattga cagcaaaagt attggtgtgt
     4981 tttggctctt aagcattaag aaacatttat tcagtaaaaa tgaagtaagt cttctaaatt
     5041 ctattggaaa tcagattgct atatctattg caagatctaa atcttatgat gaactaataa
     5101 aaaaaaataa atatgatgaa attattagtt ctgttgtaaa gtctgtacat agttcaatag
     5161 atcttcagca agtatttgaa aatactgttg atgcaatttt caataatatt aaaactatta
     5221 aacacgtagc agtacagatt gttgaaggta attcagcaaa tattaaggct tatagaggtt
     5281 atccaaaatg gtttataaat gaagttagaa aaatagaacg t
//
LOCUS       DBNGSV010000193        12749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_449915, whole genome shotgun sequence.
ACCESSION   DBNGSV010000193 DBNGSV010000000
VERSION     DBNGSV010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_449915"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..715
                     /locus_tag="AC159U_06880"
     CDS             161..715
                     /locus_tag="AC159U_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336820.1"
                     /translation="MNRSNHVIYPLLFTTFIINLLFSACSSGDSGKPNRDPSQWKITL
                     DIDGEEVILPLKVMDVYLVEDENYPESYYIKGNGVSLLGNFPDGVHVGYGEKWDKLFD
                     EIIQISEEGVDPYNTRASQISLPGNPDLLITDGYIVFSKLTGKYSGSSGDLTIHGTIS
                     LTVDSSGGEQTYEGTISVQCVSWG"
     gene            complement(755..3853)
                     /locus_tag="AC159U_06885"
     CDS             complement(755..3853)
                     /locus_tag="AC159U_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3336821.1"
                     /translation="MHISEFSVKNPVLMNLLMAGIIVVGLLTAFRLPLELFPSIQLEL
                     VTVTTIYPGASAEDVEQLVTIAVEDELSDIEGVKVVRSTSSEGRSYVVAELEAGEDIQ
                     KVAQDVRSDISQIRNKLPDDIEEPLISEQKASFPIISVSIAGEVKRELLRKFALDLRD
                     DLELIKGVDNVVTSGLGDPVFWIRVNPAQLRQYDISIEDISATINNKNLDFPGGSFKQ
                     QDLEYLVRSKGRIQSVADIEDLPVKVSTSGKHIYLKDIASIELGEEKTISTSRINSLP
                     AISFWVNKRKNADVVETAKRIEQKVEQFRKSLPDEIDIFLANDNSYWVKQRLKTMVIS
                     GIVGLIIVLILLNTFLDRRSAIVVAAGLPVSFFGAFIVMSFTGVTINLLSMFAMILVL
                     GIIVDDAIIVAENIQRYIQNGLSPARAAVVGTKEVALPVIATILTNIASFVPLLLASG
                     IIGKFLSIIPKVAIFALLVSLIEALLILPTHCSDYLRAIPKKRSKNRGMVYKMRSYYL
                     KALAFVLYKRYIVLGSSIVILVMSIAVYVQIPPVLFHTKDIAQFIVRVENPTRSSLGS
                     TKESVKKVESVIKENIPPHVLKNVFSMIGIDISQKVPEFGDHVASILVEYEDFEKRKE
                     NGKDLMKLVKNKSQDVIGPNIIKFIQTEGPPTGDPVDIEIQGDDFNVLKDLSADIKKE
                     LDNMPGVYGVSDNLVWGKPEIRLIVDEKKAAIYGLNNTTVSRAVRAAIDGLTVSRTRI
                     GSEEADIIVKQDLPTNDIEYILKTYEISNGRGSWVPLSNIVDFEMSPSMLSISRYDME
                     RAVRVTAEIDQDVSTSSEVNIQLQNFIKTMISKLPGYSYKVGGEDEETRESIDSIINA
                     IVVSAILIYLILATILKSYLQPFIIMSVLPYTIIGVLFGVLLRGEPVSLPALIGFVAL
                     LGVVVNDSLVLMDFINKRVKNMNKVVAVFMAAKHRFRPIVLTTITTFGGLALLMTKTR
                     GESAFLAPMAIALGFGLVFATLITLFLIPALYLILDDIKKYVGIKIEDYKDKRKLEIP
                     TS"
     gene            complement(3870..4994)
                     /locus_tag="AC159U_06890"
     CDS             complement(3870..4994)
                     /locus_tag="AC159U_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3336822.1"
                     /translation="MKQRIVSRQQWRWFAGFLLCFFIVIIALYIRMSSVRSSALESGF
                     IRKPQPVTVYDAVVSEQWTTANFLARVQGGQTIELRSEVGGWVEEKKVDIGSSVDVGE
                     VLLVLSDQRKELALIESKSRFDAAQADLKELRRLYNKNISLVEKGIVARDTLESLNNQ
                     IAAKRAEVNALEASYKRSNWDVEHLIIKSPIKGKIIDVLPDIGQEVLANEIVINIVNS
                     TKKKVVAGVDANWARTLKTDTEVKLTNTIDNEPQTSRGIIRGISPNIDAASGTYSVEA
                     EITDESNNWLPGEIIDMEVPVEKLQNVIKVPRSAILSDSKDVFLFTFTEGKAVKVPVS
                     VTWLNDKHGAIPQDSLPKDSKIIIEGHTGLADKQPVRIIN"
     gene            5248..5544
                     /locus_tag="AC159U_06895"
     CDS             5248..5544
                     /locus_tag="AC159U_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3336823.1"
                     /translation="MATVKKPLTKAQISTHIAESAGITKKAAGEILDELASLAYSETK
                     KKGEITLPGIGKLVLQKRNARMGRNPATGETIKIPAKKVVKFRVAKACKDSVLG"
     gene            5715..6095
                     /locus_tag="AC159U_06900"
     CDS             5715..6095
                     /locus_tag="AC159U_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336824.1"
                     /translation="MVKILFADDEELARSLYGESLQAVDGFEVKTAVDGNDALQKIAE
                     DKPHLVILDVFMPNKSGVEVLQALKSDPATKDIPVIMFSGVAEMNTITECLDMGAIGY
                     IQKDEEHEEIIAKIKLLVRLKVRA"
     gene            6282..6668
                     /locus_tag="AC159U_06905"
     CDS             6282..6668
                     /locus_tag="AC159U_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336825.1"
                     /translation="MTKILLVDDEQSIRTVYTEYLNRVDDFKVETAEDGNDALLKIAQ
                     SKPDIVILDISLPEISGVDVLGIIKADPEFKKIPVVILTGSQDYTEINKCLGMGAASY
                     ITKGEKHDEIIDKIKMVIKLNQRIRN"
     gene            complement(6658..7848)
                     /gene="coaBC"
                     /locus_tag="AC159U_06910"
     CDS             complement(6658..7848)
                     /gene="coaBC"
                     /locus_tag="AC159U_06910"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3336826.1"
                     /translation="MLNNKNIVVGVSGGIAAYKSCDLVRKLVLSGADVHVVMTKNAQQ
                     FVTPLTFQTLSNNKVSVDLFDLEREAEIGHITLADNADLIVVAPATASLIGKSASGIA
                     DNLLNTVILATKSPVLFCPAMNVNMYDNPIVHENITKLNKHGYYILEPSEGALACGWE
                     GKGRLPDAPIIVSEIEKILTPADLTGEKILVTAGPTREYIDPVRFISNPSSGKMGYAV
                     AKAAWKRGAEVILVSGTTDTELPYGVNAIVVESAEQMYEAVMKLSDWATIVVKAAAVG
                     DYSAKNYEKDKISKRKVTFNLELEKTRDILFEIGKNKNGKLLVGFAAETNDIVKKGKA
                     KLKEKNLDLIVVNDVTKEDSGFAVDTNSAFVLNRNGDVREYDVMPKEMLAHKIFDELL
                     KINS"
     gene            complement(7916..9622)
                     /gene="ilvD"
                     /locus_tag="AC159U_06915"
     CDS             complement(7916..9622)
                     /gene="ilvD"
                     /locus_tag="AC159U_06915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3336827.1"
                     /translation="MKKKTTTSKIDTRKYSSLIVDGADRAPNRSMLRAVGFKDKDFTR
                     SVVGVASTWSMVTPCNMHINLLADHAGRGVNGAGGKSVIFNTITVSDGISMGTPGMRY
                     SLVSREVIADSIETVAGAEGFDGIVAIGGCDKNMPGCLISISRLNRPAVFVYGGTIMP
                     GNYKGKDVDIVSVFEAVGSHANNEMSDRELKGIESCAIPGAGSCGGMYTANTMASAIE
                     ALGMSLPNSSAQAAISKDKSADCYNAGKAVVNLIKKGIRPSDIMTKKAFENAITVVAT
                     LGGSTNAVLHLLAMANAMNVKLTIDDFTRIGKRVPVLADLKPSGKYMMADFCRLGGLT
                     PLLKMLLGKGLIHGDCLTVTGKTVKENLRGVKPYPKPQDIVKPFSKPIKDSSHIVILR
                     GNLAPDGGVAKITGKEGLSFTGKAIVFDSEEACLKSILNGRVKKGHVIVIRYEGPKGG
                     PGMREMLSPTSAIMGKGLGNDVALITDGRFSGGTHGFVVGHITPEAIDGGVIAVVKNG
                     DEITIDANKHEVNLKVSKAEIKRRLSAWKKPKPKETSGVLAKYAKLVSSASDGAVTDR
                     NL"
     gene            9816..10271
                     /locus_tag="AC159U_06920"
     CDS             9816..10271
                     /locus_tag="AC159U_06920"
                     /inference="COORDINATES: protein motif:HMM:NF042610.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HIR3336828.1"
                     /translation="MKVIIEAMLSGLIGTAGMTLVMWAITKSGLAHADMIRAIGSIFT
                     RSYDDAVTPGVIVHFGVGIIIAFFYVLIISILDPSSAIKTLGAGAMIGIFHGVTFSFL
                     LVVAVAEHHPIEKFKTAGSEVAIAHLAGHIVYGLLVGAVVAATGARIIL"
     gene            complement(10266..11021)
                     /locus_tag="AC159U_06925"
     CDS             complement(10266..11021)
                     /locus_tag="AC159U_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR3336829.1"
                     /translation="MKLNKIVDFLDSYLEIDAVKDSCWNGLQFEGKEKVQKIVFAVDA
                     AVETFETAVKEKADMIVVHHGHFWKNQNPSVTSWAKDRLDILYNNKISLYACHLPLDR
                     HRAVGNNAQIIKLIGAKIKDEFMFSGNKNIGWTGEFTNSKDLSIIEKTLTDKLDADCT
                     VLPFGPKRIKRVAVCSGGAGYSGFYEALESKADLLITGDAVEVYYSAKDAGINIIFAG
                     HHATEIIGLIALSKVVNNKFHIDTKFIDLPTGL"
     gene            11076..11456
                     /locus_tag="AC159U_06930"
     CDS             11076..11456
                     /locus_tag="AC159U_06930"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR3336830.1"
                     /translation="MANKTGKTGLNRIWHALLYSISGLQVAFKNEAAFRQELFALTIF
                     SIVAVLLPVSTIVKMGLILSIVFVIIIELLNSSIERVVDLASPQQSTLAKEAKDMGSS
                     AVFLAIVCSTAFWVYILVKTFIAK"
     gene            complement(11470..12267)
                     /locus_tag="AC159U_06935"
     CDS             complement(11470..12267)
                     /locus_tag="AC159U_06935"
                     /inference="COORDINATES: protein motif:HMM:NF018066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HIR3336831.1"
                     /translation="MKVSKYLVLLLVLCITSAGSFVRADEHEMKEEAKKMELPGPQLV
                     TDAWMFITAYKADPAVLKALLPKGLEPTGHIVMNMYTVPTAEQTSGFGAYTLTYLTVE
                     LKDHDSYVMNSDITYPGRYFVHYYNSSPTMRAFTEKVGIPAQEGVTTTTVKDGKLTAV
                     LHIDGKPVITSTADVGSELGGFGGGHLNYFGLIKTEKDGKKVNQVVKYPIPFNGGAVE
                     TTNVKINFTAPEGHSLNKVKPLAAEPVWAVWMKGSFVYPQHQVINEF"
BASE COUNT         3807 a         2567 c         2129 g         4246 t
ORIGIN      
        1 cagaaaacac tttgttttgt atctaaaaga gatggaattt agatttaata acagaaatgt
       61 agatttagtt aataaaataa aggagattct gcattactta tagctctgtt ctatgcgagc
      121 tccaaatttt attctaatat cacttaaaag gtaaattgta atgaacagat caaatcatgt
      181 gatttatcct ttattattta cgacttttat tattaatctt ttattttcgg cttgttcgag
      241 cggtgatagc ggtaagccaa atagagaccc atctcagtgg aaaatcactc tcgacattga
      301 tggagaagaa gtgatcctgc ctctaaaagt aatggatgta tatcttgtag aagatgaaaa
      361 ctatcccgag agctattata ttaaaggaaa cggtgtttct ttattaggaa attttcctga
      421 tggcgtacat gtaggatatg gtgaaaaatg ggataagctc tttgatgaaa taatacaaat
      481 aagtgaagag ggagtagacc cttataacac aagagcctcg caaatatctc ttcccggaaa
      541 tcctgatctt ctcatcacag atggatatat agttttttct aaactcaccg gtaaatactc
      601 tggaagcagt ggagacctaa caatccatgg aacaataagt cttacagtcg attcttccgg
      661 tggagaacaa acatatgaag gaacaatttc agttcagtgc gtaagctggg ggtagtaacc
      721 tgcaaacatc gttgatgcta acgcagtaat aaagctagct tgttggtatt tctaacttcc
      781 tcttatcttt gtaatcttct atctttatac ccacatattt ttttatatca tcaaggatta
      841 aatatagggc gggtataaga aagagtgtta ttagagtagc aaagactaac ccaaatccca
      901 gagctattgc cattggggca agaaatgcag actcaccccg ggtctttgtc ataagcaaag
      961 ctaatccgcc aaaggttgtg attgtagtta aaactatggg gcggaacctg tgctttgctg
     1021 ccataaaaac tgcaacaacc ttattcatat ttttaaccct cttgttgata aagtccatta
     1081 gtaccagact atcattaaca actactccaa gaagtgctac aaatccaatt aatgccggaa
     1141 gtgaaacggg ctctcctctt agaagcaccc caaatagcac ccctataata gtatatggaa
     1201 gtacagacat gataataaaa ggctgcagat atgattttag aattgtggca aggataagat
     1261 atataagtat tgctgataca actattgcat taataatgct atcaatggat tctcttgttt
     1321 cttcatcttc acctccaact ttatagctgt aaccgggaag tttggaaatc atggttttaa
     1381 tgaagttttg aagctgtata ttcacctcag atgaagtgct tacatcctga tcaatctctg
     1441 ctgttaccct taccgctctt tccatatcat atcttgatat gctcaacata cttggtgaca
     1501 tttcaaaatc cactatattt gaaagtggca cccaactgcc tctgccgttt gaaatttcat
     1561 aggtttttaa aatatattct atatcatttg taggcaggtc ctgtttaacg attatatctg
     1621 cttcttcgct tcctattctt gttctggaaa ccgtaagacc gtcaattgct gcccttacag
     1681 ccctggaaac tgttgtatta tttaacccat aaatagcagc cttcttttca tctacaataa
     1741 gacgaatttc aggctttccc catacaaggt tatcagacac cccataaaca ccgggcatat
     1801 tatccaattc cttttttata tccgccgaaa ggtcttttaa gacattgaaa tcatcaccct
     1861 gtatttctat atctacaggg tcacctgttg gagggccttc agtctgtata aactttataa
     1921 tattaggacc aattacatcc tgtgacttat tttttaccag tttcataagg tccttaccgt
     1981 tttcctttct cttctcaaaa tcttcatact caactaaaat cgaggccaca tgatcaccga
     2041 attcaggtac tttttgagat atatcaattc caatcataga aaatacattt tttagaacat
     2101 gaggtggaat attttccttt ataacagatt ctactttttt cacagattct ttagttgaac
     2161 caaggcttga tctggttggg ttttctactc ttactataaa ctgagctata tcctttgtat
     2221 gaaaaagaac tggggggatt tgaacataaa ctgcaatcga cataaccaat attacaattg
     2281 aagaccccag gactatgtac cttttgtata gtacaaacgc taatgcttta aggtaatagc
     2341 ttctcatctt ataaaccatt cctctgtttt ttgatctctt tttaggtatg gctctaaggt
     2401 aatcagaaca gtgcgtgggt agtattagta atgcttcaat gagcgaaacc aatagggcaa
     2461 atattgctac ttttgggatt attgaaagaa actttcctat tattccagaa gccagcagca
     2521 gaggtacaaa tgatgcaata ttcgtaagaa tagttgctat tacgggtagt gccacttctt
     2581 ttgttccaac aactgcagcc cttgcaggac taaggccgtt ttgtatatac ctttgtatgt
     2641 tttcagcaac aattattgca tcatcaacta taattcccag tactaaaatc attgcaaaca
     2701 tagagagcag gttaattgtt actcctgtaa aactcataac aataaaagct ccaaaaaatg
     2761 atacaggaag tcccgcagct acaacaatag cggatcttcg gtctaaaaat gtgttaagca
     2821 gtattagtac aatgataaga cctacaattc cgcttataac cattgttttt aatctctgct
     2881 ttacccagta tgagttatca tttgctaaga atatatctat ctcatcggga agactcttcc
     2941 tgaattgttc tactttttgc tctattctct ttgcagtttc taccacatcc gcattttttc
     3001 tcttatttac ccaaaatgat atagccggca gactatttat tcttgaagta gatatggttt
     3061 tttcttcacc aagctcaata ctggcaatgt cttttaagta tatatgcttt ccacttgtgc
     3121 ttactttaac cggaaggtct tcgatgtctg cgacactttg aattcttccc ttcgagcgca
     3181 caagatattc aaggtcctgc tgcttgaaac tacctcccgg aaaatccaga tttttattat
     3241 ttatagttgc tgagatgtct tcaattgata tatcgtactg cctcagttgt gcgggattta
     3301 ccctgatcca aaatactgga tctccaagtc ccgaagttac tacattatcc acacccttta
     3361 taagctccag atcatctcta agatccagag cgaacttcct aagcagttct cgtttcactt
     3421 ccccggcaat tgatacactt ataattggaa aactggcttt ttgctcgcta attaaaggtt
     3481 cttctatatc atctggaagc ttatttctta tctgtgagat atctgatcta acatcctgtg
     3541 ctactttttg aatgtcctcc cctgcttcaa gttctgcaac tacataagat cttccttccg
     3601 aagaagtaga ccttacaacc tttactcctt ctatatcact aagctcatcc tctacagcta
     3661 tagtaaccaa ctgctcaaca tcttcagcag aggcgccggg atagatggta gtaaccgtaa
     3721 caagttcaag ctgtattgaa ggaaatagtt caagaggcag tctaaatgcc gtaagtagtc
     3781 ccactactat gatcccagcc ataagcaggt tcatcaatac tgggtttttg actgaaaatt
     3841 cagatatatg cacttagatg aagtattagt taattgatta tccttacagg ctgcttatcg
     3901 gcaagtcctg tatgaccctc aattattatt ttggaatctt tgggaaggct gtcctgtggt
     3961 attgctccat gcttgtcatt tagccatgta acactaaccg ggaccttaac tgcttttccc
     4021 tcagtaaatg taaatagaaa tacatcttta ctatcagata atattgcact tcttggaact
     4081 tttataacat tttggagttt ttccaccggc acttccatat ctattatttc accgggcagc
     4141 cagttgttgg attcatcggt aatttccgcc tctactgaat aagtgccgct tgcagcatca
     4201 atattaggac ttattcccct tattatacct ctcgatgttt gcggctcatt atctatagta
     4261 tttgtaagtt tcacttccgt atctgttttt aatgttcttg cccagtttgc atcgacccct
     4321 gcaactactt ttttctttgt actgtttact atattaatca caatctcgtt tgctaatact
     4381 tcctgtccaa tatcaggcag tacatctatg attttgccct ttataggtga tttaattata
     4441 agatgttcga catcccagtt tgatcttttg tatgatgcct caagagcatt aacctctgcc
     4501 cttttagcag caatctggtt attaagagac tctagtgtat cccttgccac tattcctttt
     4561 tctacaaggc ttatattttt gttgtagagt cttctaagct ctttgaggtc agcctgtgcg
     4621 gcatcaaatc tggatttaga ttcaattaat gcaagttctt ttcgttgatc acttagcacc
     4681 aacaatactt cgccaacatc tacactgctc ccgatatcta cctttttttc ttctacccag
     4741 ccgcctacct cggatctgag ctcaatagtc tgtccaccct gaaccctggc aagaaagtta
     4801 gccgttgtcc actgctccga aactacggca tcatacacag taaccggttg tggttttctt
     4861 ataaaaccac tttcgagtgc tgatgatctt actgaagaca tgcggatata tagagcaata
     4921 attactataa aaaaacatag taaaaaaccc gcaaaccacc tccactgttg tcgactaact
     4981 attctttgtt tcatatctac taattatatc ctaatatatt tctgatgtat ctaaagtatc
     5041 actatattat tactaactga aaaatgtcat taatgtttta ttttctttac agtctgccta
     5101 atcaacaatt tttccgggtt aataattttc tattcatatt gatattataa caaatcatta
     5161 atttattatt tcaattcaat aaataatcta ttgattttta aaataaattt ctgtactatt
     5221 cagttaaacc agccgtaagg aggaacgatg gcaacagtta aaaaaccttt aaccaaagcc
     5281 cagatttcaa ctcatattgc agaaagtgca ggaattacca aaaaagctgc aggtgagata
     5341 cttgatgaac tagcttcact agcttacagt gaaactaaga aaaagggtga gataacactt
     5401 ccaggcatag gcaaacttgt attacaaaag cgcaatgcca gaatgggaag aaaccctgct
     5461 acaggcgaga ctataaaaat ccctgctaag aaagttgtta agttcagggt agccaaagcc
     5521 tgtaaagatt cagtcttagg atagatacta cataaacctt aatattcatt aagttaaggt
     5581 cattattgtg ttttatagtt ccagttaggt acgggagagt tgtgctgttt ttatatcaaa
     5641 agttgatata atattttcac ccgccctgta aatacgctca aatttgattt agactactac
     5701 acaaagggct tggcatggta aaaatattat ttgcagatga tgaagaatta gctagaagcc
     5761 tgtatggtga aagccttcag gctgttgacg gttttgaggt aaagaccgca gtagacggca
     5821 atgatgctct gcaaaaaatc gcagaggaca agccccacct tgttatatta gatgtattca
     5881 tgcccaacaa atctggtgta gaagtcttac aagcactaaa atctgacccc gccactaagg
     5941 acataccggt aattatgttt tccggagttg ctgaaatgaa tactattaca gaatgtctcg
     6001 atatgggtgc cataggctat atacaaaaag atgaagaaca cgaagaaatc attgcaaaaa
     6061 taaagcttct tgtaagactt aaggttaggg cctaaccctc caaactcctc atattgttat
     6121 taaattcaac tagttagccc tctttttaga tatcgataat atacattttt attttttaac
     6181 aatagttttt ttctcaaagt tttattaata cattgattaa ttcttaagta gggattaaaa
     6241 tttatctact ttacttactg ttaacagcat acttttatta tatgactaaa atcttgttag
     6301 ttgacgatga acaatctata agaacagttt ataccgaata tttaaacaga gttgatgact
     6361 ttaaggtcga aaccgctgag gatggcaatg atgcacttct aaaaatagcc cagagtaaac
     6421 ctgacatagt aattttagat atatcacttc ccgaaatttc cggagtagat gtattgggga
     6481 ttattaaagc agatccggaa tttaaaaaaa tcccagttgt tatacttaca ggttctcagg
     6541 attatactga aattaataaa tgccttggta tgggtgctgc aagctatatt accaaaggcg
     6601 aaaagcatga tgaaataata gataaaataa aaatggtaat aaagctgaat caaaggatta
     6661 ggaattaatt tttagtagtt catcaaatat tttatgagct aacatctctt ttggcattac
     6721 atcatactct ctaacatcac catttctatt taaaacaaaa gcactattag tatctactgc
     6781 aaatccagaa tcttctttag taacatcatt tacaacaatc aaatccaggt tcttttcttt
     6841 gagtttggct ttgccttttt taactatatc gttagtttct gcagcaaaac ctacaagtaa
     6901 tttaccgttt ttattttttc ctatctcaaa taaaatatcc ctggtctttt caagttccag
     6961 attaaatgta acttttcttt ttgatatttt atccttttca taattcttag ctgaataatc
     7021 acctaccgct gctgccttta ctactattgt agcccaatct gatagtttca tcacagcttc
     7081 atacatttgc tcagcagatt ctacaacaat tgcgttcact ccatacggaa gttctgtatc
     7141 agtagtacct gaaaccagta tcacctcggc accccttttc cacgcagcct ttgcaacagc
     7201 atagcccatc ttgccacttg agggatttga aataaatctc acaggatcaa tatactctct
     7261 tgtaggaccg gctgtgacta aaatcttttc acctgttaaa tcagcagggg ttaatatttt
     7321 ttctatttct gaaactatta tcggagcatc gggtagcctt cccttaccct cccagccgca
     7381 tgcaagagca ccttcagatg gttccaatat ataatagccg tgtttattaa gtttggttat
     7441 gttttcatgc actatgggat tatcatacat gtttacattc attgcaggac aaaaaagcac
     7501 aggtgatttt gttgctaaaa ttactgtgtt tagcaaatta tcagcaatac cagaggctga
     7561 tttcccaata agtgatgcgg ttgcaggagc tacaacaatt aggtcagcat tgtcggcaag
     7621 tgtaatgtga cctatttctg cttctctttc cagatcaaat agatccactg atactttatt
     7681 gttagaaagg gtttgaaagg ttagaggggt tacaaactgc tgggcattct tggtcattac
     7741 aacatgcacg tctgctccgc taaggacaag ttttcttacc agatcacatg acttatatgc
     7801 agcaataccc ccgcttactc caaccactat atttttgtta tttaacatat tcatatcttt
     7861 ataatagtat acatactaat taatcatata ctgctctatt caattctata aaactttaaa
     7921 gatttctatc agtaaccgct ccatctgaag cagatgaaac aagctttgca tatttagcga
     7981 gtactcccga agtttccttt ggcttgggtt ttttccacgc tgaaagacgc ctcttaattt
     8041 cagccttgga tacttttaaa ttaacctcgt gcttgtttgc atctatggta atttcatctc
     8101 cgttttttac cactgctatt actccgccgt ctattgcctc aggagtgata tgaccaacaa
     8161 caaacccgtg tgtccctcct gagaaacgtc cgtctgttat taacgctacg tcgtttccaa
     8221 gtcctttgcc cataattgcg gaggtagggc ttagcatttc tctcatcccg ggcccgccct
     8281 ttggtccttc atatcttatt acaataacgt gtcctttttt tacccttccg ttgagaatgg
     8341 attttaagca ggcttcttct gaatcaaaca ctatagcctt tcctgtaaaa gaaagccctt
     8401 ccttacctgt tatctttgct actccgccat caggtgccag attacctctc aaaatgacaa
     8461 tgtgacttga gtctttaatt ggtttactaa atggtttaac aatgtcctgt ggttttgggt
     8521 aaggtttaac ccctctcaaa ttctctttaa cagttttccc cgtgacagtc agacagtcac
     8581 catgaataag tccttttccc agaagcattt taagcagagg agtaagcccg ccaagtctgc
     8641 aaaaatctgc catcatgtac ttaccgcttg gctttagatc agcaagtaca ggaactctct
     8701 taccaatcct ggtaaaatca tcaattgtta atttcacatt cattgcattt gccattgcta
     8761 gcagatgcag tactgcattt gttgaacccc caagtgtggc tacaacagtg atagcatttt
     8821 caaatgcttt ttttgtcata atgtcacttg gtcttatccc tttttttata agatttacca
     8881 cggcttttcc tgcattatag caatctgcag atttatcctt ggatattgca gcctgtgcag
     8941 agctatttgg aaggctcatc cccaaagcct caattgccga agccattgtg tttgccgtgt
     9001 acatgccgcc gcacgatcct gcccccggta ttgcgcatga ctctatcccc ttaagctctc
     9061 tgtccgacat ttcgttattt gcatgtgacc caacagcttc aaaaactgag actatatcta
     9121 cgtcctttcc tttatagttg cccggcatga tagtaccgcc gtatacaaat acagcaggtc
     9181 tattaagcct tgaaattgaa atcagacacc cgggcatatt tttatcacac ccgccaattg
     9241 ctacaatacc gtcaaatccc tcagcacctg caactgtttc aatggagtct gcaattacct
     9301 ctcttgaaac taaagaataa cgcatacccg gagtacccat tgatatacca tccgatacag
     9361 tgatggtgtt aaaaattaca gactttccac ctgcaccatt cacccctctg cctgcatgat
     9421 ctgctagtaa attgatgtgc atattacatg gagtgaccat gctccatgta gaagccactc
     9481 ccacaactga ccttgtgaaa tctttatcct taaatcccac ggctctcagc atagagcggt
     9541 ttggtgccct gtcggccccg tctacaataa gtgatgaata ctttcttgta tcaatttttg
     9601 atgttgttgt tttcttcttc aattaattcc tccattaaac cttattaact cttcatctta
     9661 ctaaaatttt tcaattaatt tcataatcat actataaaac taaaaagaaa ctaaactcat
     9721 ttctattaca tcataaatat taaaaggaat aaaagtattc attttataat cattaagtag
     9781 taaaatatac atataaacgg aaagaggagg tagatatgaa ggtaattata gaggctatgc
     9841 tttccggttt aattggtact gcaggcatga cgcttgtgat gtgggcaata acaaaatcgg
     9901 ggcttgctca tgcggatatg ataagagcaa ttggaagtat tttcacccgc tcatatgatg
     9961 atgcagtaac cccgggggta attgtacatt ttggagtagg tatcataatt gcttttttct
    10021 atgttcttat tataagcatc ttagatccgt cttcagctat taaaaccctg ggtgctgggg
    10081 ctatgattgg aatttttcat ggggtaactt ttagttttct tcttgtagtt gcagtagctg
    10141 agcaccaccc catcgaaaag ttcaagacag caggctctga ggtagcaata gctcatcttg
    10201 caggtcatat agtttatggt ttattagttg gtgctgtggt agctgctact ggggcaagga
    10261 ttattttata atcctgtagg cagatcaata aattttgtat ctatatgaaa tttattgttc
    10321 actacttttg agagtgctat tagtccaata atttctgtag catgatgccc tgcaaagatg
    10381 atattaatcc ctgcatcttt ggcagaataa tacacctcta cggcatcacc agttattaag
    10441 agatcagcct ttgattcaag ggcttcatag aatccggaat aaccagcccc gccgctgcaa
    10501 actgccacac gttttattct cttaggacca aaaggcagga cagtacagtc agcatctaat
    10561 ttgtcagtaa gtgttttttc tataatagat aaatcttttg agttagtaaa ttcacccgtc
    10621 cagccaatat tcttatttcc tgaaaacata aattcatctt taatttttgc accgattaat
    10681 tttattatct gagcattatt tccaactgct ctatgtctat cgagaggaag gtggcaggca
    10741 tagagtgaaa ttttattgtt atagagaata tcaagtctgt ctttggccca ggatgttaca
    10801 gatggatttt gattcttcca aaaatggccg tgatgtacaa ctatcatatc ggctttttct
    10861 tttacagccg tctcaaatgt ctctaccgct gcatcaacag caaagacgat tttctgtact
    10921 ttttcttttc cttcaaactg aagcccattc cagcaggaat cctttacagc atctatttcc
    10981 aaataagaat cgagaaaatc tacaattttg tttaatttca ttatattaat attatatgat
    11041 taactattaa tatattttat aataattaaa tggaaatggc taataaaaca ggtaaaacag
    11101 gattaaatag aatttggcac gcactattat attctatatc aggactgcaa gttgcattta
    11161 aaaatgaagc cgcttttaga caggaacttt ttgcacttac catatttagc atagtcgcag
    11221 tgcttctgcc cgtttctact attgtaaaaa tgggacttat cctgtctatt gtctttgtga
    11281 taataataga actcttaaat tcttctattg aaagagtggt tgatcttgct tcaccccagc
    11341 agagtaccct tgcaaaagaa gcaaaagata tgggcagctc tgctgttttt cttgctattg
    11401 tctgttctac ggcattttgg gtctatattt tggtgaagac attcattgct aaataatttt
    11461 atctaaatcc tagaattcat ttataacctg atgctgagga tatacaaaac ttcctttcat
    11521 ccaaacagcc catacaggct ctgcagcaag tggttttact ttatttagtg aatgaccctc
    11581 tggtgctgtg aaatttattt tcacattcgt ggtttcaact gctccgccgt taaaaggtat
    11641 cggatatttt acaacctgat ttactttttt accgtctttt tcagtcttga tcaggccaaa
    11701 atagtttaga tgaccaccac caaacccacc aagctcactt ccaacatccg ctgtagaagt
    11761 aatcacgggt tttccatcta tatgtaaaac tgcagtaagc ttgccgtctt taactgttgt
    11821 tgtggtaaca ccttcctgag caggaatacc aactttttca gtaaaggctc tcattgttgg
    11881 tgagctgtta taatagtgaa caaaatatct accaggataa gtaatatcac tgttcatcac
    11941 ataagagtca tgatctttta gctcaaccgt tagatatgtg agtgtgtacg cgccaaagcc
    12001 agaggtctgc tctgctgttg gtactgtgta catattcata acaatatgac ctgtaggctc
    12061 taaaccctta ggaagaagtg cttttaaaac tgctggatcg gctttataag cggtaataaa
    12121 catccaggca tccgttacaa gctgtggacc aggaagttcc atcttttttg cttcttcttt
    12181 catctcatgc tcatccgctc ttacaaatga gccggcagat gtaatacaaa gtactaataa
    12241 taaaacaaga tattttgaaa ctttcatatt tttacccctc caattgatct gttaataaag
    12301 aaatattgaa ttgttaagac ctaaaagtca ataaaattaa tggttattga agcttttagt
    12361 atttaatcga ttattgacaa taaaattaat ttgttataac atagacataa tatagcttaa
    12421 tacttaagca tggtattgat acttaagtaa taactattaa actggcgcat taagaggggg
    12481 tgatgtagag acataaaatt atatactgaa ttcttttgct ttgtgtggat ttagtaaatg
    12541 aattcttatt ttttaaccga caaattcaca ataaaatcaa caaataaatc ttgggaggat
    12601 ttaataaatg agtattaaaa aaataggagc tcgcatagaa cagagctata agtaatgcag
    12661 aatctccttt attttattaa ctaaatctac atttctgtta ttaaatctaa attccatctc
    12721 ttttagatac aaaacaaagt gttttctgt
//
LOCUS       DBNGSV010000194        30082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_172626, whole genome shotgun sequence.
ACCESSION   DBNGSV010000194 DBNGSV010000000
VERSION     DBNGSV010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 30082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30082
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_172626"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="AC159U_06940"
     CDS             <1..1102
                     /locus_tag="AC159U_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR3336832.1"
                     /translation="LHFSADAPLAINALLFIPQENTERFGLGRTEPGVNLYCRKVLID
                     SKPQGLIPEWLRFLKGVVDSDDLPLNISRESMQDTSLIEKLNKAVTNRFLKFLEDKAK
                     KETESYEEFYKMFGVYLKEGIATDFSNREQLAKLIRYESSHTEAGKLTSLTDYVSRMG
                     QDQKEIYYLYGPSREIIENSPQMEAFKANKLEVLYLLEPVDEFVMNNIGKFEEKDLVS
                     ADSSEVKLDDVKKEKAKDGLKKKESDELCKWISDELGDKVKEVSVSKRLVDSPVIALN
                     ADKVMTPSMRKIMKSIHKEIGQSDNEINLEINPNHGLIKNLSKLKDSDAELAKLVTEQ
                     LYDNALVAAGFIEDPRSMVNRVYELLERVSAN"
     gene            complement(1110..1562)
                     /locus_tag="AC159U_06945"
     CDS             complement(1110..1562)
                     /locus_tag="AC159U_06945"
                     /inference="COORDINATES: protein motif:HMM:NF018404.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HIR3336833.1"
                     /translation="MVCFEVYVNDKKVFTAGHEDLDEITTSLKYQKDKETVKLSINGE
                     LIGNDSLRGYARWLIWVNNNKARSMRELKIGDEIKIIVNKDVIPDKPAFYKSGKEESS
                     SEIKKFCSFCSKERKEVRTLIASEHKNTYICGECIDKFREIKEEVNKS"
     gene            complement(1654..2943)
                     /gene="nuoF"
                     /locus_tag="AC159U_06950"
     CDS             complement(1654..2943)
                     /gene="nuoF"
                     /locus_tag="AC159U_06950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3336834.1"
                     /translation="MPELKIIRNYTERENSHTLQSYLSSGGYKALAQALKMQPEEVID
                     IIKKSGLRGRGGAGFPTGIKWGFIPKDTNKPVYLCCNADESEPGSFKDREIMEQDPHQ
                     MLEGIAIACYAIGSHKAYIYIRGEMPYAAKIITEAIEEANTKAYLGKNVNNSGYDIDI
                     VLFRGAGAYICGEETALLESIEGKNGEPRPKPPFPAQVGLFGCPTIINNVETLAFVPH
                     IINNGAEWFLGIGKPKNTGTKIYGLSGHVNKPGLYELPLGIPIGQLIEEYGGGVPNGR
                     KVKAMSPGGSSSAILDASELDMPMDFDSLAAVGSMLGTAGVTVMDDQTCMVRVAQNLA
                     HFYRDESCGQCVQCREGTWWLEMMLTKIEAGKGSMEYIDTLLDACHQMRGTTICALAD
                     GCAMPIESIINKFRAEFEDHITSGKCPFENKHMGDWG"
     gene            3168..4355
                     /locus_tag="AC159U_06955"
     CDS             3168..4355
                     /locus_tag="AC159U_06955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3336835.1"
                     /translation="MKTDFDIIIVGGGPSGLSAASFLSDKGYSVALFDRNYVIGSDVI
                     CSGVISNEVFDRYDLPTSSIVAKLKDAELVSPYGSTLQYTHPEQSVSVVDRHKFDFEL
                     FQIAKQKGARIYTGANVRSIIKNEHCIEAEVAFDNKVNKYRSKLLVLATGVRFNLHPK
                     LGFGRPKKILKAIQVEIDEILSDKLKLFWGSQYSDGFFGWSIPLDDGRTRVGVMTESN
                     SRECIGNLLTELNYDQQRNRGCINFKQRGISFGSIEKSYSDRVIAVGEAAGLVKTTTG
                     GGIFYGILSAEIACDVIDRCFTEDDFTENTLSDYESGWKNIIGNEIKHGQYFHNFFSK
                     LSDESIDELFEAVNKDGLFDYISRSGNFDWHSGTVVKILRSPNIRRVLLNGFVSSKLK
                     LAI"
     gene            complement(4352..5557)
                     /gene="lhgO"
                     /locus_tag="AC159U_06960"
     CDS             complement(4352..5557)
                     /gene="lhgO"
                     /locus_tag="AC159U_06960"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HIR3336836.1"
                     /translation="MDKSECDVLIIGSGITGLTIANELIKRNYENIIIIDKESHTGTH
                     ASGRNSGVLHSGVYYSPGTLKARFCVEGNHLMTKYCEDNNLPINTVGKVIVATDEKKL
                     ENLYELKRRADHAGAVANIINEKELAEIEPNASTLQKALYCPETSVVKPTEILNHLTN
                     ALRATGKVEIKYKTSFHGLIGNSAARTSGGEIRYKKIINAAGAYAEKIAQKFGLAKDY
                     NLIPFKGTYKKLKNGASELVRCNIYPVPDLNNPFLGVHFTKSVDGTVYAGPTAIPALS
                     RENYNLFENLDGETLEILLRDGLLTLFNRSFRSAAVNEIKKYFDHYFYSEAKTLLPIL
                     KPSDLENSTKVGIRPQLVHWPTKKLVMDFIVLGDGDSVHVLNAISPGFTTSMAFSKYV
                     VDILEGKAD"
     gene            5737..6231
                     /locus_tag="AC159U_06965"
     CDS             5737..6231
                     /locus_tag="AC159U_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3336837.1"
                     /translation="MLKIGENGVYPAHGVVEVVGIESKEFSGTAKQFYILKVLDNEVT
                     VMVPTDNANTVGLRPLVAKNKVPRVYNILGNKDKVNTSNNNGNQSWNKRYREYADKLK
                     SGDIFEVASVLREIKLLQNGKELSFGERRIMDSALGLLIKEISLSKNQKEDKVETEIK
                     KLLG"
     gene            complement(6288..7697)
                     /locus_tag="AC159U_06970"
     CDS             complement(6288..7697)
                     /locus_tag="AC159U_06970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3336838.1"
                     /translation="MSKFNRCIFIMADGARPDVFAQLLSKGDLPNISKYVVEKGTCTN
                     AVTVFPSTTGPAYTPYLLGKFPGRCNFPGIRWFDKKEFSKTFFSYKRFRSYIGYETYL
                     MNRDISTEHKTIFEIIPDSLSILNEISRGITAGRDKTKYSKIYYKLKSHFTEKTNEVD
                     EVSRRIILDHSTNLPEFSFVVFLGIDNYSHLTHPFHKHVMDSYSIIDNTVGKLAKTLL
                     ETNKLDDTLILIVSDHGLTQTHSHFDLLEFMNDSGYKTLYYPYVFHNFIGATAANMVS
                     GNSMSNLYFKGESNWNGFNVRDLNGFENKLVQRPEIDILLCRQPDNRIKIMSKRGSAR
                     TWIDSENRIGYEVTDGDPFGYKSLPAVMNSTESLQLSYNTDYPDALLQINQLFESPRA
                     GDIVVSANLGYDLRAKHENPEHGSSHGSLHRDHMMVPIAINADINSEYLRTVDLFPTV
                     LELLGYDVPDALDGKSLVN"
     gene            complement(7783..10548)
                     /gene="glnE"
                     /locus_tag="AC159U_06975"
     CDS             complement(7783..10548)
                     /gene="glnE"
                     /locus_tag="AC159U_06975"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR3336839.1"
                     /translation="MTDVYPDEVNAGNTLDIFRQVNKKVSKKKEDILFHLSSYSRFIG
                     REITRNPTILDYLVTSKYTKNHKPYEELLNDANEISNKSQNINQFQSEIKRYKYKEFA
                     RIILKDIRDSSVFVQTMQELSDLASAIVSSVLFFHSKDDTSIWDNFVVIAMGKLGGRE
                     LNLSSDIDLIYLFDGTENLEPIFKFAENITKTLSNRTEDGFLYRVDLGLRPGGGKSPI
                     AVSLEGSLEHYNYWGDTWERGALIKARPIAGNLELGNRFLEEVENFIYKRHLDYASIE
                     DIKEMKFKLDSLHKVGDVKLGKGGIREIEFFVNAFQLIHGGTLKQLRSNSLLKTLEIL
                     TELKFISDDIRQTLESSYIFLRKTEHFIQLVEEKQTHALPTKSEDLQLLAKRVGLKNE
                     KEFAKEYSRITNNVSEIYNKLFLESSQKIEQSGKEFWELADFLTEGHISEEQATSKLS
                     ELGFKDPKTALIIIEILLNPKKGGLTQRGRSLSRKIIPVLLKYVISSVNPDVSLRNVD
                     RFLSSIGSRSSIYSVLLENPEICALLSKLFSTSAYLSAFLIKHPEYLDILTLRSVWKD
                     YKSSEEMMAELSDTVKKEPDFEGKLDSLRRFKHVETLKLSMMYLNGELEPGYLGNYIS
                     MIADAVVNVGLEVALKEMKPAKSRKKEIVVIGMGKLGGMEMGYNSDLDVIFVYNGDDH
                     EYYSKLGQKLISVLSIPTGEGFAFKIDMGLRPSGNAGALVSSLESFKNHHKKGAMLWE
                     RQALLKARVIAGDKQLAKKVMSTIESFVYKQPLQDESIKEISRLRKRMELELAKETKT
                     KFNIKTGHGGLVDIDFIVQLLQLKHGAKLKIIRQTNTIEALASLFNEAIISESEFKTL
                     DTGYKFLRRIENAVRLIQDKATSDIQIDDFNKAGEIIFNTSGESLLKEYKSVTKKIRK
                     IYNSYFS"
     gene            complement(10541..10822)
                     /locus_tag="AC159U_06980"
     CDS             complement(10541..10822)
                     /locus_tag="AC159U_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336840.1"
                     /translation="MKKKRKSDLNTARAHFQNAAKEGLIASAYMLKGMREMSKSKNYR
                     KQILENTGEFLDQGLDLFIKFAEAVNECSQSSEKSSSKSKKKSRKIEID"
     gene            complement(10916..13342)
                     /gene="priA"
                     /locus_tag="AC159U_06985"
     CDS             complement(10916..13342)
                     /gene="priA"
                     /locus_tag="AC159U_06985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR3336841.1"
                     /translation="MKRAAKVAFPIPNSEHYHYRIPDDLKDLVALGHRVLVPIKSRKA
                     IGYVIGLEKPPADIKLKNIIDVLDEKPLFDKKRLSFFKWISDYYLAPIGMILKSAHPG
                     GLGLNVNKVISITEQGKSALDSEATDLTELQILNALSAGSLSINKILEIVQGSTNAVI
                     NKLKLKNLVQTEYALDRKSYLKTERFITSSDKSRNKELFDIICKKRPAKAKILEYILR
                     NENIEYPQLKEVFGNLSGHLKWLEENGYIEIETKELIRDPYSEIKITREKPVELNVDQ
                     KLCLKELKSASNSKSFSPFLLHGVTGSGKTEVYLQATQHVLNKGREALVLVPEISLTP
                     QLVKRFKARFGNKIAVLHSAVNESERFDSWQKISEGKIKIAIGARSSIFAPFKNLGII
                     VVDEEHEQSYKQEESPHYNARDLALVLGKIYRSVVVLGSATPSVETYSNSLKEKVKYL
                     SLPLRATNSTMPRVEIVDIKKEKNIVISEPLRKAIWENFSSGNQTIIFLNRRGFSSVL
                     VCRACGETLECPNCSITLTFHKSDSSVKCHYCGLDEGKFNKCLKCGNLLIEYGIGTQK
                     VEEEIKQIVPDARCARMDRDEVRGKKGLIDLYNKLQKNEIDVLIGTQMVAKGHDLAGV
                     TLVGVISADMSLSIPDFRAGERTFQLLTQVSGRAGRGSKEGRVIVQTLKPDNSVIKFA
                     AGQDSISFLKNELKLRSALGYPPFAKLVNIKFTARTDSQTSGIANTTRAIASKLLQKF
                     PLDSIEILGPSPCPISKIKNKYRWQLLIKSSDPKELHKFAKGLQKALRLAKFPVGIRF
                     GFDIDPMSFN"
     gene            complement(13536..14162)
                     /gene="msrA"
                     /locus_tag="AC159U_06990"
     CDS             complement(13536..14162)
                     /gene="msrA"
                     /locus_tag="AC159U_06990"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3336842.1"
                     /translation="MMKKIVGILCVFAILYSLPAILSGDDEDVEVNLEVYEKATFAGG
                     CFWCVQPPYDKLEGVIKTTVGYTGGKEQNPTYKQVAYGKTTHTEAIEVIYDPTVVGYD
                     KLLEVFWMNIDPTDLGGQFVDRGSQYRPEIFYHSEEQRIIAKNSKEKLNNSGRFDRPV
                     VVPITEATTFWKAEEYHQKYYKKSPVGYYAYRKGSGRDQFINKYWPSK"
     gene            complement(14217..15332)
                     /locus_tag="AC159U_06995"
     CDS             complement(14217..15332)
                     /locus_tag="AC159U_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3336843.1"
                     /translation="MELAIFIVVAVIFLAYSNGANDNFKGVATLFGSRTLDYKKALYW
                     ATATTFFGSLFSIYLSNGLISAFSGKGLVSPEVISNTSFVISIGLGAASTVMLATVIG
                     IPVSTTHSLIGALVGAGIASVGSVNLSNLGQSFFLPLAVSPFLSFGLTALVYPVFKKT
                     RQTLGVERQTCVCVSEGSYAPVMMRTDGSVVLKSSGIQIAAGNLDDCEQRYNGKLLGI
                     GAQTVLDWLHFLSSGMVSFARGLNDTPKIVALLVSAKAVGIDLKLAMVIVGVAIACGG
                     LLSAKKVASTMSDKIVPMNHGQGFTANLITALLVTVASKFGIPVSTTHVSCGSLFGLG
                     AVTKQGRWKVIRTIIFAWFITLPVAALFSSIVYFIVS"
     gene            complement(15458..16255)
                     /locus_tag="AC159U_07000"
     CDS             complement(15458..16255)
                     /locus_tag="AC159U_07000"
                     /inference="COORDINATES: protein motif:HMM:NF018684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR3336844.1"
                     /translation="MIKNILPGLLLLAVLIIPCISNSSPLSECNKESENKKELSKCLD
                     AKLKKAEASLDNKVLEIRKKMEQKSIKSNQIYPIRSFNLSQQSYLIYRKTNCNWYFDK
                     SFPGSGSSELLKNCLIKMTLDRILELDSSIETVNPSADKTDKSSSSSNNDNNNNRSNA
                     AKTNTSSNKKHNNDTDNIRQQQRDSDNRKHSDNNNRAADTNYADTDKYDNLDERCYIK
                     PDPGTCEEFIMKYFFYPEGGNCSSFIWSGCGGVVPFESMAECKEICQ"
     gene            complement(16442..16786)
                     /locus_tag="AC159U_07005"
     CDS             complement(16442..16786)
                     /locus_tag="AC159U_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3336845.1"
                     /translation="MEIKEITTDESITHLELIGRLDLKGVEQVELKFTTLVAAKAKPS
                     LIDLSKLEFIASLGLRMFLSCAKTLKSKNAKLVLYKPTAQVLEILTSVGFDKLIPIED
                     DMDKAMQILKEA"
     gene            complement(16834..18243)
                     /locus_tag="AC159U_07010"
     CDS             complement(16834..18243)
                     /locus_tag="AC159U_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR3336846.1"
                     /translation="MEIKFCGAARTVTGSSHLLTLDDGYKILLDCGLYQGREEEFNDY
                     NSKWFFDPAEIDCLILSHAHIDHCGRIPKLVKDGFKGKIYSTAATRDLCAVMLMDSAK
                     IQENDAKYINKKRRKKKLPETAEPLYTSYDIVPAIEMFVGISYGIKYKIREDIVLEFR
                     DSGHIFGSASITLDLKDKDGNNKRIGFSGDIGRPNRPIIRNPMPMDDLDYLICESTYG
                     GRRHNSLPKDIEGLLKTIQHTCIENKGKLIIPAFSVGRTQEIVYTLDRLETEGKLPHI
                     PVYVDSPLSVNATEIFQLHPDCFDADILEYMLMDPNPFGFNNLKYVRSVEESKRLNRS
                     DEACIIISASGMMTGGRIMHHIFHNIDNSKNTIFIVGYCAPHTLGARVRRGDKKIKIF
                     GETKKVKASVEVVDSYSAHGDQDEMLEYLDNLDRTRLKNIFLVHGELDRQEIFREALL
                     KSGFADVQIPGLNDVGTLK"
     gene            complement(18255..18671)
                     /locus_tag="AC159U_07015"
     CDS             complement(18255..18671)
                     /locus_tag="AC159U_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336847.1"
                     /translation="MRFNSGKEYILTIFYYFTPIFILSDYLFGFNVRVSGLQNYGSYK
                     TVYYIFCLICAFLIYRYEGYSLVIGFIESIINLLILFISFLLPYFTYASIIADGNIQE
                     ITFYDSKMVINFMISGIIVIFTFQSSLNRLTNRIGL"
     gene            complement(18675..20321)
                     /locus_tag="AC159U_07020"
     CDS             complement(18675..20321)
                     /locus_tag="AC159U_07020"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3336848.1"
                     /translation="MLKSYFPIYKKSETIKYIIFALVFAVFFSIASHAVNEQQDTDSV
                     AGVIKYKLDSKHKLRIGDVLISPKTTLASFYKSRGFKPAWENMDINYLMKSLYEIETE
                     GLRKEDYFYTEIIDLIKAEESGNLAKIENKADLEILLSYSLSKLFDHLQNGKIDPKSL
                     KSEWDIDKFATPSRYMLLTKALESTNLYEFIDSQKPSLGYYNTLKKALSTYKEYKKNG
                     GWVKVAGGRILKPGMYDKRVQALRDRLLVTGHLSSSQNFETVQYDEQLVEAVKKFQLH
                     HNLITDGVIGPNTLKELNIGVDDRIDQIKVNLERARWVLNNADQKFVIVNIAGFKAYY
                     ADNGEILWTSRIQVGKPYRKTPVFKSEIQHLVFNPTWSVPPGIFRKDILPIIKRNPGY
                     LTRRGFNVYDYNGRKVSPYSINWARYSANVPYSIRQKPGRRNALGRVKFIFPNDHFVY
                     LHDTPNRSLFNKDIKTFSSGCIRVEKPFELAELLLNDNEKWNRQNIQKVVDKRKTRTV
                     FLDKPVPIMILYWTASADKNGNVYFMNDIYKRDKAILKLL"
     gene            complement(20343..20873)
                     /locus_tag="AC159U_07025"
     CDS             complement(20343..20873)
                     /locus_tag="AC159U_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HIR3336849.1"
                     /translation="MKKLKALIAYLLFYPTLLWTVFLGRVLKTRNWWDEIDDCVILGA
                     IPFSSDVSRLSEMGVKCVVNLCAESSGPTALYKKYKIDYCNIPTIDFTCPGVEAIAKG
                     VLNIERYRDKDEKVYIHCKAGRGRSATIVFCWLVCVKNYSTEQAMKLLIDKRHQVNKK
                     LHNRQHVKKYIDQNSN"
     gene            complement(20870..21235)
                     /locus_tag="AC159U_07030"
     CDS             complement(20870..21235)
                     /locus_tag="AC159U_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3336850.1"
                     /translation="MSVKNINEIESEEVSAGKDTTRQVLIGSDQAPNFAMRRFVIEPG
                     GSMPRHKNSVEHEQYVLGGRARIGIGDKTYEVKKDDTVFIPAETPHWYECLGDEPFVF
                     ICVVPNAEDKIELVEQSSC"
     gene            complement(21271..22518)
                     /locus_tag="AC159U_07035"
     CDS             complement(21271..22518)
                     /locus_tag="AC159U_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336851.1"
                     /translation="MNGLYYHFGIITYLILIFFVASCSVPKTITESKQSKSLEKAIQL
                     SAKYLQKSVKENGMFEYRINTDPTIEVKQKYNILRHAGTIYAMSQYLELYPNDKEIRT
                     AIEQAGRYLQNKAIEQVPGKKEMLAVWSKPEINNSGRPLEVKLGGTGLGLVALLSIEK
                     FHQEFTPLSDLRILGNFIVYMQREDGSFYSKYIPAEGGRSDKWTSLYYPGEAALGLLM
                     LYEKDKSALWVESAFKALKYIAQKNSDAVVVDHWYLIATEKMLNLNNYNLTDQQRDLL
                     ISHAARICEKIIDEQIISHKYSEFRGGFAVDGRITPSSIRLEGLLAASAFLPNEYREF
                     MRINSAIDRGISFLQRAQIEEGQYAGAYRRALRKLDGDTVTIRNFNRRVTEIRIDYVQ
                     HALSALLGYVNYSASRNMHNSIS"
     gene            complement(22543..22878)
                     /locus_tag="AC159U_07040"
     CDS             complement(22543..22878)
                     /locus_tag="AC159U_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336852.1"
                     /translation="MKKQLAITVFSLLSLTTYIPYVASDDSEKWFLMSRHGECAEIKT
                     LQRKIPDIGGINDPESFIKLMEEKGNEVIKNEPKELKGKAVEVSVPNEGLSLMFVVES
                     VCVGFASKE"
     gene            complement(22971..23138)
                     /locus_tag="AC159U_07045"
                     /pseudo
     CDS             complement(22971..23138)
                     /locus_tag="AC159U_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(23154..23270)
                     /locus_tag="AC159U_07050"
     CDS             complement(23154..23270)
                     /locus_tag="AC159U_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3336853.1"
                     /translation="MAEIRWTLQATGDLEAIAEFISRDSAYYARLFVSKIIS"
     gene            complement(23258..23452)
                     /locus_tag="AC159U_07055"
     CDS             complement(23258..23452)
                     /locus_tag="AC159U_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336854.1"
                     /translation="MALAKTEKEVMVDAIKSLPDDTSFEEAMERLYLLSKVERGLRQA
                     DNGETISHEQVIKRLDKWLK"
     gene            complement(23525..24013)
                     /locus_tag="AC159U_07060"
     CDS             complement(23525..24013)
                     /locus_tag="AC159U_07060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3336855.1"
                     /translation="MSEIKVRKAKVEDAVSMAEILREIGWSERRNQMKLEEVSGPVEG
                     LIDHCNKDTEGHTMYVATDEKDEVIGFTTVHWVPFVMLGSFEGYISDVFVSPRAGGKG
                     AGSLLVKTVMEEGKERGCMRLMLTNGKEKPSYKQGFYKKLGWTERPKVANFVYYYREP
                     WS"
     gene            complement(24028..25062)
                     /locus_tag="AC159U_07065"
     CDS             complement(24028..25062)
                     /locus_tag="AC159U_07065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3336856.1"
                     /translation="MTDISLEKKIAQMLMIGFDGYEVDENHHISEAIGKYNLGGVILF
                     DLEEKDYRLKNIESHEQVQNLTANLQSYSDNNLLIGIDYEGGQVNRLKEEYGFPKTFS
                     QQHLGELNDPSLTYEKSAEMAGTLSGLGINLNFAPVVDLKINPENPIIAKRERSYSSD
                     PEVVTLHSKEFIKAHRQAGVLTCLKHFPGHGSATADSHHGFVDISDTWSESELIPYKK
                     LVKDNLVDCVMSAHVFNRNIDPKYPATLSSIHIDQELRNEIGFNGVVFSDDLHMGAIK
                     DNYTFEQTLNLAINAGIDILIISYDEEYKENLCENLIYTILRLVKDGSISQSRIDESY
                     DRIILLKNKL"
     gene            complement(25101..25382)
                     /locus_tag="AC159U_07070"
     CDS             complement(25101..25382)
                     /locus_tag="AC159U_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336857.1"
                     /translation="MTAKIIGAIWPLIVFPAVLFLTIQGTLDLGGGEKDIVIAIPMLA
                     FTLIYLVSYVILTKKKKRTFIAVMLSAGISLVLLFVITLVFAQLLGVKA"
     gene            complement(25733..26494)
                     /locus_tag="AC159U_07075"
     CDS             complement(25733..26494)
                     /locus_tag="AC159U_07075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3336858.1"
                     /translation="MKLKGKTALITGGGDGIGRATALLFCKEGAKVGIMGRTKSSLNK
                     VKKEAMGKGEIHVLQGDVSNEKDVKRVVASFQRKYKKIDILFNNAGIFEAGTAVTTTN
                     DIWNRTINTNVKGVFLMSKYVVPIMAKGGGGAIVNNSSVLGIVGMEDCLAYNASKGAV
                     RQISKSMALDHAKENIRVNSVCPGYIKTKMDVEFMGNPPDADEILDKIAADMIPLIRR
                     AEPEEVAHSVLYLSSDEARYMTGSDLVIDRGWTTL"
     gene            complement(26529..27011)
                     /locus_tag="AC159U_07080"
     CDS             complement(26529..27011)
                     /locus_tag="AC159U_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR3336859.1"
                     /translation="MPSFDIVSEIDLHEVTNAVDQANKEVATRFDFKGTDSKYELTDN
                     IVTLNTKEEFQLGQMMDVLSKKLSTRKVDVRCMDKDEPELSSNTARQKVTLRHGIETE
                     LAKKMVKMIKDTKLKVQASIQEKQVRVNGKKRDDLQAVMAMLKDSNLEIPLQFINFRD
                     "
     gene            complement(27037..27633)
                     /locus_tag="AC159U_07085"
     CDS             complement(27037..27633)
                     /locus_tag="AC159U_07085"
                     /EC_number="3.1.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HIR3336860.1"
                     /translation="MNYLAHTYLSDNSEESILGNLLGDFIKGNPDEHYEGEILKWIKI
                     HRKIDAYTDAHSVFRSSKRLISKERRRFAGIIVDISFDHYLAKNWHEYSNETFPQYVN
                     KIYSILQKNKSILPERLIGILPRLVSENWFDMYKSIEGIDYVFNRMSKRVKRENSLAG
                     SVVEITQNYSELEDNFFCFFPEVINFVKQLNDVNNNVK"
     gene            complement(27643..27975)
                     /locus_tag="AC159U_07090"
     CDS             complement(27643..27975)
                     /locus_tag="AC159U_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336861.1"
                     /translation="MSKKIISVYHKPTNTKLAEGPGGWGITSFEGNLYISRKYLLTEG
                     FKINYFPGFCIYKFFYFWMDLNLEDGHIIKNLAWMYVLPNPLFPFIWFRVGIPCYHPE
                     IEIIENVN"
     gene            complement(28001..28225)
                     /locus_tag="AC159U_07095"
     CDS             complement(28001..28225)
                     /locus_tag="AC159U_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336862.1"
                     /translation="MKRFLWITVPIVLMVLVFYLFFRPVCVIITEEEVKNFNPPIETR
                     TDKNFYMQTFQKKNGQWYQCKTWISRKFFF"
     gene            complement(28222..28455)
                     /gene="nrtS"
                     /locus_tag="AC159U_07100"
     CDS             complement(28222..28455)
                     /gene="nrtS"
                     /locus_tag="AC159U_07100"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HIR3336863.1"
                     /translation="MTLSIFFKNFFRKDTTLRALKVAFVVAPILILINHFDTIMNRDF
                     HWVFYLKSIITFFVPYTVSAFSSAMAYSQSVGK"
     gene            complement(28512..29462)
                     /locus_tag="AC159U_07105"
     CDS             complement(28512..29462)
                     /locus_tag="AC159U_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIR3336864.1"
                     /translation="MRNIFILSVIILLLTTYNSYSISLFEDYNGFDVSNSLIPKEKIF
                     SGGPPKDGIPAILNPKFESASSASWLKSSDLVSGVEMEGVFKAYPLRILVWHEAVNDK
                     VGSKPIMVSFCPLCGSTLVFDRNIDGQELTFGISGLLYQSDVLFYDHQNQSLWSQLEM
                     EAISGPKAGTEMGVLPSVLATWGEWKKKHPNTLVLSRDTGFNRDYSNMPYGGYEKSTS
                     LYFPVDNQDKRYHPKEKVLVVISSDGVAKAYAFKELGKVSTPLKDQVGSDKITIKYND
                     GKFVSAYDNNGSPIKSFVTYWFAWYTFRPDTLVYSVSESG"
     gene            complement(29512..29961)
                     /locus_tag="AC159U_07110"
     CDS             complement(29512..29961)
                     /locus_tag="AC159U_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3336865.1"
                     /translation="MPYTLIKKLHTYRGESKFSSWLYRVTANASYMMLRSEKKYEYDL
                     SLEDYVPYDNKGTLMGRMHHKDWSSRPDIVIYVKEALKILEKAVDELPEVYRVVFYLR
                     DIEGLTNEEVAEVLDISVPATKSRIHRSRLYIRDKVSDYFYEWKKIG"
BASE COUNT         8850 a         6142 c         5141 g         9949 t
ORIGIN      
        1 gcttcacttc agcgccgacg caccgcttgc gataaacgcc ttattgttca taccgcagga
       61 aaacacagag cggtttggtc ttggcaggac tgagcctgga gtaaatttgt attgccgtaa
      121 agtgttaatc gactcaaagc ctcaggggtt gattcccgaa tggctcagat ttttaaaggg
      181 tgtagtagac agtgacgacc tgcctcttaa tatctcaagg gaatccatgc aggacacctc
      241 actcattgaa aaactcaata aagccgtaac gaacaggttt ttaaaattct tagaagataa
      301 agccaagaaa gagaccgaaa gctatgaaga gttctataag atgtttggtg tatatttgaa
      361 agagggtata gccactgatt tttccaatag agaacagctt gcaaaactca taagatatga
      421 atcttctcat acagaagccg gaaaacttac ctctcttact gactatgttt ccagaatggg
      481 acaggatcaa aaagagattt attaccttta tggcccaagc agagagataa ttgaaaacag
      541 ccctcagatg gaggcattca aggcaaacaa actcgaagtc ctttatttgc tggaacctgt
      601 agatgagttt gtgatgaaca atataggcaa gtttgaagag aaagatctgg tatcggcaga
      661 cagctcagag gttaaacttg atgatgttaa gaaagaaaaa gccaaagacg gacttaaaaa
      721 gaaagaatca gatgagcttt gtaaatggat aagtgatgag cttggagata aggtaaaaga
      781 ggtaagcgtg agcaagaggc ttgtggatag tccggtgata gcgctaaacg cagataaggt
      841 tatgactcca agcatgcgca agatcatgaa atcgatccat aaagagattg gccagagcga
      901 caatgaaatt aatctggaaa ttaatccaaa tcatggactt ataaaaaatc ttagcaaatt
      961 aaaagattct gatgcagagc tggcaaagct tgtaaccgaa cagttatacg ataatgccct
     1021 tgttgctgca ggttttattg aagacccgag gtctatggta aacagggttt atgagctgct
     1081 cgagagggta agcgctaact aagttataat tatgatttat ttacttcttc tttaatttct
     1141 ctaaacttat ctatacattc accacagata taagtatttt tgtgttcact tgctatgagt
     1201 gtcctgactt ctttacgttc tttagaacaa aatgaacaga atttcttgat ctcggaagat
     1261 gattcttctt tacctgattt ataaaatgca ggtttatccg gtatcacatc tttgttaact
     1321 ataattttaa tctcatcacc aatttttaat tctctcatac ttctggcttt gttattattt
     1381 acccaaatta accatctcgc atagcccctt agagaatcat ttccaattaa ttcgccatta
     1441 atggataatt tcactgtttc tttatctttt tgatatttga gcgatgttgt aatttcatcc
     1501 aaatcttcat gccccgctgt aaacactttt ttatcattta catatacttc aaaacaaacc
     1561 atagcacacc ctcgaagcaa tattgtagtt ataaaaagta taaataagat ataagaaaag
     1621 tagaaattta tttaggggca ggcttttgta gtactatccc caatcgccca tgtgtttgtt
     1681 ttcaaaaggg catttgccgg aggttatgtg atcttcgaac tcggcacgga atttgttgat
     1741 tatggattct atgggcatgg cacagccgtc ggcaagggcg catattgtag tacctctcat
     1801 ctgatgacag gcatcaagca gggtatctat gtattccatt gatcccttac cggcttcaat
     1861 ttttgtaagc atcatctcaa gccaccatgt accctctctg cactgtacgc actgtccgca
     1921 ggattcatcc ctgtaaaaat gtgcgagatt ctgggcaacc ctaaccatac aggtctgatc
     1981 atccattact gttacacccg cagttccaag catcgaaccc actgccgcaa gggaatcaaa
     2041 atccatgggc atatcaagtt cagatgcatc cagtattgct gaagacgagc ctccgggaga
     2101 catggccttc actttccttc catttggaac tccaccgcca tattcttcta ttagttggcc
     2161 tattggtatc ccaagcggaa gctcataaag cccgggttta tttacgtgac cgctaaggcc
     2221 atatatcttt gtaccggtgt tcttgggttt tcctattcct aaaaaccatt ctgcgccgtt
     2281 atttattata tgcggtacaa acgcaagggt ttctacatta ttaataatag ttggacatcc
     2341 aaaaagaccc acctgagcag gaaatggagg ttttggtctt ggttcaccat tcttaccctc
     2401 gattgattcc agtagggcag tttcttctcc gcaaatataa gcgcctgctc ctctgaacag
     2461 gacaatgtct atatcatagc cggagttatt aacatttttt ccaagataag cctttgtgtt
     2521 ggcttcttca atcgcttcag tgattatttt tgcagcatac ggcatctcac cacggatata
     2581 aatgtacgcc ttatgagaac caattgcata gcatgcaatc gctatgccct ccagcatctg
     2641 atgaggatcc tgctccatta tttccctgtc tttaaaactt ccgggttcac tctcatctgc
     2701 attacaacac aggtaaaccg gcttgttggt atcctttgga ataaatcccc atttaatccc
     2761 cgtaggaaat cctgcccctc ctcttcccct taggcccgac ttcttaatga tatcgatcac
     2821 ttcttcaggc tgcattttca gtgcctgcgc aagcgcttta taacctccag aagataagta
     2881 agactgtaga gtgtgtgaat tttccctctc tgtgtaattt ctaataattt ttagttcagg
     2941 cattaaattt atttcctcag ggttttaaaa tgagaactta ttatatatcg ataaatcacg
     3001 attccaataa ataaatttca attattttat taatattatt gtgatctcaa taatttgttc
     3061 tattagtaca ctaagccttt agttttagta atattgagtt gacttttatg gttactaata
     3121 cattttttca tgtttaaatc atatcaattt tccagagata aatacaattg aaaacagatt
     3181 ttgacataat aatagtaggg ggaggaccaa gcggattatc agcagcttct ttcctgtcag
     3241 ataagggtta tagtgtagca ctgttcgaca ggaactatgt aattggcagt gatgttatct
     3301 gctctggtgt tataagtaac gaggtattcg atcgttacga ccttccaaca agttcaattg
     3361 tagcaaagct aaaagatgcc gagcttgttt caccctatgg aagcacgctt cagtatactc
     3421 accccgaaca gtcagtatcg gtagtagaca gacataaatt tgattttgaa ctttttcaaa
     3481 tagctaagca aaagggtgct cggatatata caggagctaa tgttagatca atcataaaaa
     3541 atgaacactg tattgaggca gaagtagcat ttgacaacaa agttaacaaa tacagatcaa
     3601 agctgttagt gcttgctaca ggcgtgaggt ttaaccttca tccaaaactt ggttttggaa
     3661 gacctaagaa aattctaaaa gcaatacagg ttgaaataga tgaaattctt agtgacaaat
     3721 taaaactctt ttggggaagc cagtattcag atggtttctt tggatggtca attcctcttg
     3781 atgacggcag gacaagagtg ggagttatga cagaaagtaa ctcaagagag tgtatcggaa
     3841 acctgcttac tgaactaaac tatgatcagc agagaaatcg tggctgcatc aattttaaac
     3901 agcgcggaat ttcatttggt tcaatagaaa aaagctactc ggatagagtt atagcagttg
     3961 gtgaagctgc cgggcttgta aaaactacta ccggcggggg aatattctac ggtatcctta
     4021 gtgccgagat cgcttgtgat gttatagaca gatgttttac tgaagatgat tttaccgaaa
     4081 acacattaag tgattatgaa tctggctgga aaaatataat aggaaatgaa atcaagcacg
     4141 gacagtattt tcataacttc ttttccaagc ttagcgacga atccattgac gaactttttg
     4201 aagccgtaaa caaggacggt ctttttgatt atatctcaag aagcgggaat tttgactggc
     4261 acagtggaac agttgtaaag attctaagaa gccccaacat tagaagagtt cttctaaatg
     4321 gatttgttag tagcaagctt aagcttgcta tctagtctgc cttaccctca agtatatcca
     4381 ccacatattt tgaaaaagcc attgatgtag taaaacccgg tgatattgcg tttaagacat
     4441 gaaccgaatc tccatctcca agtactataa aatccatcac cagttttttt gtcggccagt
     4501 gtacaagctg tggtcttatg ccaacctttg tgctgttttc cagatcagaa ggttttaata
     4561 ttggaagcag agtctttgcc tcagagtaaa aataatgatc aaaatatttc tttatttcat
     4621 ttacagctgc actcctgaat gacctgttaa atagagttaa gagtccatcc cttaatagaa
     4681 tttcaagtgt ttctccatcc agattttcaa ataaattata gttttctcta cttagtgcag
     4741 ggatggcagt aggacctgcg taaacagttc catcaacact tttagtaaaa tgaacgccca
     4801 aaaaagggtt attgagatcc ggtacagggt agatattgca ccttacaagc tcagaagccc
     4861 cgttctttaa ttttttatat gttcctttaa aaggaataag attatagtcc tttgcaagtc
     4921 caaatttctg tgcaatcttt tctgcataag ctccggcagc atttataatt tttttatacc
     4981 ttatttcccc tcccgaagtt ctggctgcgc tatttccaat aagaccgtgg aatgaagtct
     5041 tgtatttaat ttcaaccttt cctgttgctc taagagcatt agtaaggtga ttaagaattt
     5101 cggtaggctt tactacagaa gtttccggac agtatagtgc tttttgcagg gtagacgcat
     5161 ttggttctat ctcagcaagt tctttttcat ttatgatatt tgcaactgca cccgcatgat
     5221 ctgcccttct ttttagttca tacaggtttt ccagtttctt ttcgtctgtg gctacaataa
     5281 cctttccaac ggtattgatt gggaggttgt tatcctcaca gtactttgtc atgaggtgat
     5341 tgccttctac acaaaacctg gctttgagag tgcctggtga atagtacacc cctgaatgca
     5401 gtaccccgct gtttctgccg cttgcgtggg tacccgtatg actttcctta tctatgataa
     5461 taatgttttc atagtttctt ttgataagct catttgcaat agtaagccct gtaattcctg
     5521 agccaattat gagcacatca cattctgatt tatccattat gtttaattaa aaaataagtc
     5581 atcaatacta tggagtatct acccaatacg cttaagtcat caaaattatt agtaaattac
     5641 aaatattata ttatttctct gtttgaccat caccctaatt tatgctactt tattagttct
     5701 agtcaagggg ttttaaatct tacaaggtaa taattaatgc taaaaatcgg agagaacgga
     5761 gtatatcctg ctcatggggt agttgaagtg gtaggtatag agagcaagga attcagtggt
     5821 acagcaaaac agttttatat cctaaaggta ctcgacaacg aagtaactgt aatggtacct
     5881 actgataatg caaatactgt ggggttaaga cctcttgtag caaagaacaa ggttccgcgt
     5941 gtatataata tccttggaaa caaagataaa gttaatacca gcaacaataa cggaaaccag
     6001 agctggaata aacgttacag ggaatatgcc gacaaactca aaagtggtga tatttttgag
     6061 gttgcttccg tactaagaga gataaagtta cttcaaaacg gcaaggagct ttcttttggt
     6121 gagcgtagaa taatggactc tgcactcggc ctgcttataa aggaaatatc actgtccaaa
     6181 aatcaaaaag aagataaagt agaaacagaa atcaagaagc tgcttggtta aatctattaa
     6241 attttctttc tctaaatcca actcagtaat taattcttaa aaagtagtta gtttactagg
     6301 ctctttccgt ctaaggcatc cggcacatca tatcccaaga gctcaagcac tgtaggaaac
     6361 agatctactg ttctgagata ttcactattt atatctgcat ttatggcaat tggaaccatc
     6421 atatgatccc tgtgcagtga accatgggaa gagccgtgtt cgggattttc atgcttagcc
     6481 ctcaggtcat aaccaagatt tgctgatacg acgatatccc ccgcccttgg tgactcaaac
     6541 aattggttta tctgtaaaag tgcatcggga tagtctgtat tatagcttaa ttggagtgat
     6601 tctgtgctgt tcataacagc cggcaatgat ttataaccaa atggatcacc atctgtaact
     6661 tcatatccaa ttctgttttc tgaatcaatc catgtgcgag cactgccgcg tttactcata
     6721 attttaattc tattatcagg ttgtctgcaa agaagtatat caatctcagg tctttgtacc
     6781 aatttgtttt caaaaccatt taaatcacgt acgttaaatc cattccagtt tgattcaccc
     6841 ttaaaataaa gattggacat agaattgccc gataccatat ttgccgctgt agcaccaatg
     6901 aaattatgaa aaacatatgg atagtagaga gttttatacc cactgtcgtt cataaattcc
     6961 agcaaatcaa aatgtgagtg agtctgagtg agcccatgat cacttacaat aagtataaga
     7021 gtatcatcga gcttattagt ttctaaaaga gtctttgcaa gctttccaac tgtattatct
     7081 attatgctgt aagaatccat aacatgtttg tgaaatgggt gagtaaggtg agagtaatta
     7141 tcaataccaa gaaaaaccac aaacgaaaac tctggcagat tggtgctatg atccagaata
     7201 atgcgcctgg atacctcatc cacttcgttt gttttttcag taaaatggct ctttagctta
     7261 taatatattt ttgaatattt ggttttgtct cttcctgcag ttattcctct agatatttca
     7321 tttagaatgc taaggctgtc tggaataatc tcaaaaatgg ttttgtgctc tgtggatata
     7381 tcacggttca ttaaatatgt ttcatagcct atataactgc gaaacctctt gtaagagaaa
     7441 aaggttttag aaaattcttt cttatcaaac catctgatcc ctgggaaatt acacctgccc
     7501 gggaatttgc cgagtaaata aggtgtataa gccgggcctg ttgtagaagg aaaaacagta
     7561 accgcgttag tgcacgttcc tttttctaca acatattttg aaatgttggg aagatcgcct
     7621 ttacttagga gctgcgcgaa tacatctggt cttgccccat ctgccataat aaaaatacat
     7681 ctattaaatt tgctcaaaaa actatatctc ccaatttata ataatattaa ggattaatta
     7741 ctagcatgaa tgaaacataa aagtatatct tctgaaaata tattaagaaa aataggaatt
     7801 gtaaattttc cgtatttttt tagtaacaga tttatattct tttaacaggg actctccgct
     7861 tgtattaaat attatttcgc cggctttatt aaaatcatct atttgtatat cacttgttgc
     7921 cttatcttgt atgagcctaa cggcattttc gatacgcctt aaaaatttgt atcctgtatc
     7981 taatgtctta aattcactct cactaataat tgcctcatta aaaagggatg ctaaagcctc
     8041 tatggtgtta gtctgtctga ttattttaag ttttgcgcca tgctttagct gtaataactg
     8101 gactataaaa tcaatatcta caagcccccc atgacccgtt tttatattaa atttagtttt
     8161 agtttccttt gcaagctcaa gctccattct ttttctaaga cgcgatattt cttttattga
     8221 ttcatcttgc agcggctgtt tatatacaaa actctcaatt gtactcatta ctttttttgc
     8281 aagctgttta tctccggcaa ttactcttgc cttcagcaga gcctgccttt cccaaagcat
     8341 tgctcctttt ttatggtgat ttttaaatga ctccagagat gataccagcg ctcctgcatt
     8401 gccggaaggc ctgaggccca tatctatttt aaaagcaaaa ccttctcctg ttggtataga
     8461 aagtactgat attaattttt gcccaagctt tgaataatat tcatggtcat ctccattata
     8521 gacaaaaata acatcgagat cggaattata tcccatctcc attccgccga gcttgcccat
     8581 tcctattact acaatttctt ttttcctgct tttagcaggc ttcatttctt ttagcgcaac
     8641 ctcaagtcct acatttacga ctgcatcggc tatcatcgat atgtaattgc ccaaatatcc
     8701 tggttcaagc tcgccgttta aatacatcat gctgagtttt aatgtttcta catgtttaaa
     8761 acgtcttaga gaatccagtt tgccttcaaa gtcaggctct ttttttactg tatcactaag
     8821 ctcggccatc atctcttcac tgcttttata atctttccat acactcctta gggtaagaat
     8881 atcaagatac tcgggatgtt tgattaaaaa tgctgataaa tatgcgcttg tagaaaaaag
     8941 tttcgaaaga agggcacaaa tctcaggatt ttcaagaagc acactgtata ttgaacttct
     9001 ggagccaata ctggaaagaa acctatctac gtttctaagt gacacgtcgg ggtttaccga
     9061 ggagatcaca tatttaagaa gtactggtat aatttttcta gagagcgacc tgccccgttg
     9121 tgtaaggcca ccttttttag ggtttaagag tatttcaatg ataataaggg cagtctttgg
     9181 atctttaaat ccaagctcac ttaatttact tgtggcctgc tcttcggata tatgcccttc
     9241 agttaaaaaa tcggcaagct cccaaaactc tttcccactc tgttcaatct tttgtgatga
     9301 ctccaagaac aatttattgt agatttcaga aacattgttt gtaatcctgg aatactcttt
     9361 tgcaaactcc ttttcatttt taaggccaac cctttttgca agcagttgca aatcctctga
     9421 ttttgtggga agcgcatgag tctgcttttc ttcaacaagc tgtatgaagt gttcagtttt
     9481 tcttaagaat atataagatg attccagagt ctgccttata tcatcagaga taaatttaag
     9541 ttcggttaat atttcaagtg ttttcaataa gctgtttgat cttaactgtt ttaaggttcc
     9601 accgtgaata agctgaaatg catttacgaa aaactcgatc tccctgattc ctcccttccc
     9661 aagctttacg tcacctacct tatgaagact atcgagctta aatttcatct cttttatatc
     9721 ttcaatcgat gcataatcga gatgcctttt atagataaaa ttttctacct cttctaaaaa
     9781 cctgtttcca agctcaagat ttccagcaat tggtcttgct tttataagag cccccctctc
     9841 ccacgtatca ccccaatagt tatagtgctc caaagagccc tctaaagaca ctgcgattgg
     9901 gcttttaccc ccgccaggcc ttagcccgag atcgactctg tacaaaaatc cgtcttcagt
     9961 tctgttgctg agagtttttg tgatgttctc tgcaaattta aatatgggtt ccaggttctc
    10021 agtaccatca aataaatata tcaaatcaat atctgagctt aggtttaact ctcttccacc
    10081 aagctttccc attgcaataa ctacaaagtt atcccagata ctcgtatcat ctttggagtg
    10141 gaaaaagagc actgagctta caatggctga tgccagatct gaaagttctt gcattgtctg
    10201 cacgaacacg ctgctgtctc ttatatcttt taggattatc cttgcaaatt ctttgtattt
    10261 gtacctttta atttcagact gaaactggtt tatgttctgc gatttattgg atatctcgtt
    10321 agcatcattc agcagctctt catatggttt atgattcttg gtatattttg aggttactaa
    10381 atagtccaaa attgtggggt ttcttgtaat ttctctgcca ataaatcggc tgtaagagga
    10441 taaatggaac agtatatcct cttttttttt agatactttt ttgtttacct gcctgaatat
    10501 atcaagagta ttaccggcgt ttacttcatc tggataaaca tcagtcaatt tctattttcc
    10561 ttgatttctt tttagattta gaagatgatt tttcagaaga ttgagaacat tcatttacag
    10621 cttcagcaaa cttgataaac agatcaaggc cctgatcgag gaattcaccg gtattttcca
    10681 gtatctgttt tctgtaattc ttgctcttag acatttctct catacccttt aacatataag
    10741 cagaggcaat gaggccttct tttgcagcat tttgaaaatg agctcttgca gtatttagat
    10801 cagattttct ttttttcttc atattctaac ccctcactac taaatttata tctacaaata
    10861 gaagtttaac acattatcga aaacactaaa cttaattaca gataagctat acagactaat
    10921 taaagctcat gggatctata tcaaaaccaa atcttattcc cactggaaac ttggcaagcc
    10981 ttagagcctt ttgcaggccc tttgcaaact tgtgcaattc ttttggatca gaggatttta
    11041 taagaagctg ccaccggtat ttatttttta tcttactgat cgggcaggga gaaggaccaa
    11101 gaatctctat ggaatccaga ggaaatttct ggagaagttt tgatgcgatt gctcttgtag
    11161 tattagcgat acccgatgtc tgactgtccg ttcttgccgt aaatttaata ttaacaagtt
    11221 ttgcaaaagg gggatagcca agagcagagc gcagttttag ctcatttttt aaaaagctaa
    11281 tagagtcctg cccggcggca aatttaataa cagagttatc aggttttagc gtctgaacta
    11341 ttaccctgcc ttccttactt cccctgccag ccctgccaga gacctgggta agcagctgga
    11401 aagtcctctc acctgccctg aaatcaggaa tagaaagtga catatcggca gatattaccc
    11461 ccacaagtgt cactcccgcc aggtcatgtc cctttgctac catctgtgtt ccaataagaa
    11521 catcaatttc atttttttga agtttattat atagatcaat taatcctttt ttacccctca
    11581 cttcatccct gtccattctt gcgcaccttg catcgggcac aatctgtttt atttcctctt
    11641 cgaccttttg cgttccaatt ccatattcaa taagtaaatt tccgcattta aggcatttat
    11701 taaatttgcc ttcatcaaga ccacagtagt gacattttac agagctgtct gatttgtgaa
    11761 aagtaagggt aatgctgcag ttgggacact ccaaagtctc tccacatgcc ctgcatacta
    11821 ataccgatga aaacccacgc ctgtttaaaa aaattattgt ctgatttcca ctgctaaaat
    11881 tttcccaaat agcttttctt aatggctctg atatgactat gtttttttcc tttttaatat
    11941 caacaatttc aacccttggc attgtgctgt ttgtagctct tagcggaagg cttagatatt
    12001 taaccttctc ttttaaagaa tttgaatagg tttcaaccga gggggtggct gaacccagca
    12061 caacaacaga tctataaatc ttaccaagca cgagcgctaa gtcgcgggca ttgtagtgag
    12121 gggattcttc ttgtttatac gactgctcat gctcttcatc aactactatt attccaaggt
    12181 ttttaaatgg tgcaaaaatg gaagaccttg cccctatggc aatctttatc ttcccttcag
    12241 aaatcttctg ccaggagtca aacctctcac tttcattaac cgcgctgtgg agtactgcta
    12301 ttttatttcc aaatcttgcc ttgaatcttt ttacaagctg aggggtaaga gagatttctg
    12361 gtacaagcac aagagcttcc ctgcctttgt ttaaaacatg ctgggtagcc tgcaagtaaa
    12421 cctccgtttt accactgcct gtaaccccgt ggagaaggaa tggggaaaat gatttgctgt
    12481 tgcttgcgct tttaagttct tttaggcaca acttctgatc aacattaagc tcaacgggtt
    12541 tttctcttgt aattttaatc tctgagtaag gatcgcgtat cagttctttt gtttctattt
    12601 ctatataccc gttttcttca agccacttta agtgtccgga aagattgcca aacacttctt
    12661 ttagctgggg gtactcaatg ttttcatttc tcaatatata ttcgagaatc tttgccttag
    12721 cgggccgttt tttgcaaatt atgtcaaata attctttatt cctgctctta tcggaagatg
    12781 taatgaatct ttcggttttt aaataggatt ttctgtccag agcatattca gtctgtacta
    12841 aattctttaa tttaagctta tttataacgg cgtttgtact gccctgcact atctccaata
    12901 ttttatttat gcttaaactt cctgcactaa gtgcgtttag tatttgaagc tctgtaaggt
    12961 ctgtagcctc cgaatcaaga gcggattttc cctgctcggt tatgctgatc accttattta
    13021 cattcaggcc aagccccccg ggatgggccg actttagaat catacctatg ggcgcaagat
    13081 agtaatctga aatccattta aaaaaagaaa gtcttttctt gtcaaaaagc ggcttttcat
    13141 ccagtacatc aatgatattt tttaacttta tatcagccgg tggtttttca agacctatta
    13201 catacccgat agccttcctg ctcttaatcg gcacaagtac cctgtgcccc agcgcaacca
    13261 ggtcttttag atcatccggt atccggtaat gatagtgctc agaattggga ataggaaaag
    13321 ctactttggc cgctcgtttc attagtttga ttaatcagta gacattagat tatgattact
    13381 ttacctaatt acaagcggag ttatagaagc aggccagaca aattgtaata ttctataaac
    13441 gttgtttctc gcttaaaaat ataatgtaaa aattggaaga ataggggaat ttatagaaat
    13501 aataaataat atcttttaaa aagtattgat cgcttttatt ttgaaggcca atacttgtta
    13561 ataaactgat ccctgcctga gccttttctg tatgcatagt agccaacagg gctcttcttg
    13621 tagtatttct ggtgatattc ctcggctttc caaaatgtcg tagcctccgt tatgggaacc
    13681 actaccggtc tatcaaacct gcccgagtta ttaagttttt ccttagagtt tttagcaatg
    13741 atcctctgct cttcactatg ataaaagatt tcaggcctgt actgcgagcc cctgtctaca
    13801 aactgacctc ccaggtctgt cggatcaata ttcatccaga aaacttctag aagcttatca
    13861 tagccgacta ccgtgggatc ataaattact tcaattgcct cagtgtgtgt ggtcttgcca
    13921 taggcaacct gtttatatgt ggggttttgc tcttttccac ccgtataacc tacagttgtc
    13981 tttataaccc cttcgagctt gtcatatggc ggctgcacgc accaaaaaca gcctcctgca
    14041 aatgttgcct tttcatatac ctctaaattt acttctacat cctcatcatc ccctgaaaga
    14101 atggccggca gggaataaag aatggcaaat acacatagaa ttcccactat ttttttcatc
    14161 attatctcca aaatttatat atatgagact agattaacga ataaggatgc aaatatctag
    14221 cttacaataa aataaacaat ggaagaaaaa agagctgcta caggaagggt tataaaccag
    14281 gcaaaaataa tagtacgtat tactttccaa cggccctgtt ttgtaactgc tcccagccca
    14341 aacaggcttc cgcaggaaac atgagtcgta gacacgggaa taccaaattt agaggccacg
    14401 gtaaccaaaa gtgctgttat caggtttgct gtaaatccct gaccatgatt catgggcacg
    14461 atcttatcac tcatggtaga ggccactttc tttgcgctta gaagtcctcc acaagctata
    14521 gccaccccta ctatgaccat ggcaagctta agatcaatac ccacagcctt tgccgatact
    14581 aaaagtgcaa ctatttttgg cgtgtcattg agaccccttg caaaactcac catcccggat
    14641 gacaaaaaat gcagccagtc taaaacagtc tgggcaccaa tacccaggag ttttccatta
    14701 tacctctgtt cacaatcatc cagattgcca gcagcaatct gaatcccaga actttttagc
    14761 actacagagc cgtctgttct catcatcaca ggcgcatatg aaccttcaga tacacacaca
    14821 caggtttgtc tttcaacacc aagggtctgt cttgtttttt taaatacagg atatacaaga
    14881 gctgtgagac caaaactcag aaatggactt acagccagag gcaaaaagaa gctttgtccc
    14941 agattagata aatttacgct tcctacagat gctatacctg ctccgactag ggcacctata
    15001 agagaatgtg tggtcgaaac aggaattcca ataactgttg caagcattac ggtactagct
    15061 gcacccagtc ctatagaaat tacaaatgag gtgtttgaaa ttacctcggg tgatacaagc
    15121 cccttgccac tgaaggcact gataagtcca tttgataaat atatcgaaaa gagcgatcca
    15181 aaaaacgtag tggcagttgc ccagtatagt gcttttttgt agtcaagagt tctacttcca
    15241 aaaagcgtag caaccccttt aaaattatca tttgctccgt tgctgtatgc tagaaaaata
    15301 accgcaacta ctataaatat tgcaagttcc atgtgttaat aaccaattaa tgtaaatagg
    15361 atgaataaaa ccggaaaaaa gatgcaattt acttaagtaa ttaaaaaaag gtacttattc
    15421 agcaggacat cttcaaaaat cagtaatttc tattatgtta ttgacagatt tccttgcatt
    15481 ctgccattga ctcaaatggg actacaccac cacagccact ccagataaat gagctgcagt
    15541 taccgccttc tggataaaag aaatacttca taataaactc ttcacaagtt ccgggatcag
    15601 gctttatata gcacctttca tccaaattgt catatttatc cgtatctgca tagtttgtat
    15661 cagcggcacg gttgttgtta tccgagtgtt ttctgttatc tgaatctctt tgctgctgtc
    15721 ttatgttatc tgtatcatta ttatgttttt tatttgatga tgtattggtt ttagcagcat
    15781 tgctacggtt gttattatta tcgttattcg aactggagct actcttatca gttttatctg
    15841 ctgatggatt tacagtttct atactgctgt cgagctctaa tatgcggtca agcgtcatct
    15901 ttataagaca gtttttaaga agttcacttg agcccgaacc tggaaatgac ttatcaaaat
    15961 accagttaca gtttgttttt ctataaatta gatatgactg ctgggagaga ttaaatgatc
    16021 tgattggata tatttggttt gattttatgc ttttttgttc catcttctta cgaatttcca
    16081 agactttgtt gtccagactt gcctcagctt tcttaagctt tgcatcaaga catttggaaa
    16141 gttctttttt attttcagat tctttattgc attcactaag tggtgaagaa ttggaaatgc
    16201 agggaataat caatacagca agaagtagaa gacctggtaa aatatttttt atcatttttt
    16261 ttcttggtat atagattgga tatttatccc gttattttat acatttaacc attaagagta
    16321 aagaaattag atgcactcac tgattggtat ttaaaataaa acaacgcagc ataataatta
    16381 ttaaaccgct tagcagtttt tttgtttcaa taacatatta tcaataaaag aataaaacta
    16441 tttaggcctc ttttagtatc tgcattgcct tatccatatc atcttcgatt ggaattagct
    16501 tatcaaaacc cactgatgta agaatttcca gcacctgggc agtcggttta tagagtacga
    16561 gctttgcatt cttgctttta agcgtctttg cacagcttaa aaacattcta agtccgagtg
    16621 aggctataaa ctcaagcttg cttaaatcaa tgagactagg ctttgccttt gcggctacaa
    16681 gtgtagtaaa tttaagctcc acctgttcta ctcccttaag gtctaaccgt cctataagtt
    16741 ccagatgtgt aatactttca tcagttgtta tttcttttat ttccataata cacttcccag
    16801 tatatataaa atttgttaat acaatattta agattatttc aaagttccta catcattcaa
    16861 tccgggtatc tgcacatctg caaatccact cttcagtaaa gcttccctga aaatttcctg
    16921 tctgtctaat tcgccatgta ctaaaaatat gtttttaagc ctggtgcggt ctaggttatc
    16981 aagatactca agcatctcat cctggtcacc atgagcgctg tatgaatcaa caacttctac
    17041 tgatgccttt accttttttg tctctccaaa aatttttatt tttttatcgc cccttctaac
    17101 tcttgcgcca agagtatggg gagcacagta tcccacgatg aaaatcgtgt ttttactatt
    17161 atcaatatta tgaaaaatat gatgcatgat cctgccgcct gtcatcatcc ccgaggcgct
    17221 aatgataata caagcctcgt cactgcggtt aagtctttta gattcctcta cgcttcttac
    17281 atattttaga ttattaaatc caaacgggtt tggatccata agcatatatt ccagtatatc
    17341 agcatcaaaa cagtcgggat gaagctggaa aatctcggta gcatttacag atagagggct
    17401 gtcaacatag acaggtatat gtggaagctt tccctcggtc tcaagcctat caagcgtgta
    17461 tacgatttcc tgagttcttc ctacgctaaa tgcaggaatt atgagctttc ctttattttc
    17521 tatacaagta tgttgaatgg ttttaagcaa tccctcaata tcttttggaa gcgaattatg
    17581 cctgcgcccg ccgtaggtcg attcacaaat cagatagtcc agatcatcca ttggcatagg
    17641 gtttcttata attggcctgt ttggcctgcc tatgtctcca ctaaaaccga tccgcttatt
    17701 atttccgtct ttatctttga gatcaagcgt aatgctggcg ctgccaaaaa tgtgaccgct
    17761 gtctctgaat tcaagaacta tatcctctct aattttatac ttgataccgt aactaatacc
    17821 cacaaacatc tctattgcag gaactatgtc ataagatgta taaagcggtt cagcagtttc
    17881 gggcagtttc ttttttcttc tctttttatt tatatactta gcatcatttt cctgtatctt
    17941 ggcactgtcc ataagcataa ctgcacatag atcccttgta gccgcggttg aataaatttt
    18001 tcccttaaac ccgtctttaa caagtttggg gattctgcca cagtgatcga tatgtgcatg
    18061 ggaaagaata agacagtcaa tttcagcagg gtcaaaaaac cacttgctgt tataatcgtt
    18121 aaattcctct tcccttccct gatagaggcc gcaatccaaa agtatcttat agccgtcatc
    18181 aagggttaat agatgactgc tccctgtcac tgtccttgca gcaccgcaga attttatctc
    18241 catagtttgc cctattataa accaatcctg tttgttaatc tatttaatga actttggaat
    18301 gtgaatatta ctatgattcc ggaaatcata aaattaataa ccattttaga gtcataaaaa
    18361 gtaatctcct gaatattccc atctgcaata atgcttgcat aagtaaaata tggaagaagg
    18421 aagctaatga ataatattag aagattaatt atgctttcaa taaaaccaat tactaaggaa
    18481 taaccctcat atctatagat aagaaatgca cagataaggc agaatatata ataaacagtt
    18541 ttataagaac cataattttg cagacctgaa accctgacat taaaaccaaa caaataatct
    18601 gaaagaataa atataggggt aaaataataa aatattgtta gaatatattc ttttccagaa
    18661 ttaaatctca taggttatag taatttcaag atagctttat cccgcttgta aatatcattc
    18721 ataaaataca cattgccatt tttatccgca gaggctgtcc agtatagaat cataatgggg
    18781 acgggtttat cgaggaatac cgtcctggtt tttcgtttat caacaacctt ttgaatattt
    18841 tgccggttcc acttctcatt atcatttagc agtaattctg caagctcgaa tggtttttcc
    18901 acgcgaatgc aaccggagct aaatgtttta atgtccttat taaacagcga tctgttggga
    18961 gtatcatgaa ggtaaacaaa atggtcgttt ggaaaaatga atttaaccct cccaagcgca
    19021 tttcttctgc cgggtttttg ccttatacta tagggaacat ttgctgaata tcttgcccag
    19081 ttaatagaat agggagatac tttccttccg ttataatcat aaacattaaa gcctcttctt
    19141 gtaagatatc cgggatttct tttgattatg ggcagtatat cttttctgaa aattccgggt
    19201 ggcacagacc atgtgggatt gaatacaagg tgctgaattt cggatttaaa aaccggtgtt
    19261 tttctatatg gttttccaac ctgaattctt gaagtccaca gtatttcacc gttatcagca
    19321 taataagctt tgaatcctgc gatattaact atgacaaatt tttgatctgc attatttagc
    19381 acccatcttg cacgctcaag gtttacttta atctgatcaa tcctgtcatc tacgcctata
    19441 tttaattctt ttagtgtatt aggaccgatc actccatctg tgataaggtt atgatgaagc
    19501 tgaaatttct ttacggcttc tacgagctgc tcatcgtact gcacagtctc aaaattttga
    19561 gaactggata gatgaccagt aacaagcagt ctgtctctca gtgcctgtac tctcttgtca
    19621 tacattccgg gcttaaggat tctgccacca gcaaccttaa cccagccgcc gtttttctta
    19681 tattccttat aagtgctaag agcctttttt aaagtattat aataaccaag acttggtttt
    19741 tgcgaatcta taaattcata aagatttgta gattcgagcg cctttgttag aagcatatac
    19801 ctacttggag ttgcaaattt atctatatcc cactcagatt tgagactttt gggatcaatt
    19861 ttaccgtttt gcaaatgatc aaatagtttt gacaaggaat aggatagcaa tatttcaaga
    19921 tcagccttat tttcaatctt tgccagattt ccggattctt ctgctttaat taaatcaatt
    19981 atctctgtat aaaaataatc ttctttccgc agtccttctg tttctatttc ataaagagac
    20041 ttcatcagat aatttatgtc catgttctcc caggcaggtt taaatccccg ggacttataa
    20101 aaacttgcaa gtgttgtctt tggtgaaatt agtacatccc caatacgaag tttatgtttg
    20161 gagtccagtt tatatttgat tacacctgca accgagtctg tatcctgctg ctcatttacc
    20221 gcatgagatg caatgctaaa aaatacagca aagactaaag caaaaattat atattttata
    20281 gtttcagatt ttttgtaaat tgggaaataa gattttaaca tcaatgaata acagtaataa
    20341 tatcagttag aattttggtc tatatatttt tttacatgct gtctattatg aagtttttta
    20401 ttaacctgat gccttttatc aattaaaagt ttcattgcct gctctgtgct atagttttta
    20461 acacatacaa gccagcaaaa cacaattgta gcgcttctgc cgcgtcctgc cttacaatga
    20521 atgtacacct tctcatcctt atccctatac cgctcaatat tcaaaacacc ttttgcaata
    20581 gcttcaacac ctgggcatgt aaaatctatt gttggtatat tacaataatc aattttatat
    20641 tttttgtaaa gtgctgtggg gccactgctt tcagcacaaa gattaacaac acatttgacc
    20701 cccatctcgc tcagcctcga gacatcagag ctaaatggga ttgccccaag gattacacag
    20761 tcgtctattt catcccacca gtttcttgtt tttaatactc tgccaagaaa aactgtccaa
    20821 agtaatgttg gataaaacag cagatacgct ataagtgctt ttaacttttt caacaactac
    20881 tctgctctac aagctcaatt ttgtcttcgg catttggcac aacacatata aacacaaaag
    20941 gctcatcacc caaacattca taccagtgtg gagtttctgc aggtataaag acagtatcat
    21001 ccttcttaac ttcataggtt ttatccccaa tgcctattct tgcccttccg cctagaacat
    21061 actgctcatg ttctacagag tttttgtgtc ttggcatcga tcctccaggc tctatcacaa
    21121 accttctcat agcaaagttg ggtgcctgat ctgaaccgat tagtacctgc cttgtggtat
    21181 ccttgccggc tgagacttct tcactttcta tttcatttat attttttaca gacatattct
    21241 taattaattt aaacgaaatt agaaatattt ctaactaatt gaattatgca tattccggct
    21301 agctgaataa ttgacataac ccaggagcgc actcaaagca tgctgaacat aatcaattct
    21361 tatttcagta acacgtctat taaaatttct tatagttact gtgtcgccat cgagctttct
    21421 tagtgctcgc ctgtaagctc cggcatactg gccttcttct atttgtgctc tttgaagaaa
    21481 tgagatgcct ctatcaatag cactattaat tctcataaat tctctatatt cattaggaag
    21541 aaaggcactt gctgcaagca gaccttccag tcgtatggag cttggtgtga tccttccatc
    21601 gactgcaaat cctcctctaa attccgaata tttatggctt atgatctgct catctataat
    21661 tttctcacat atacgtgcag catggctaat taataaatcc ctctgctgat ctgtgagatt
    21721 ataattattc agattcagca ttttctcagt ggctatgaga taccagtggt ctactaccac
    21781 agcatcgcta tttttttgtg ctatatattt tagggcttta aatgcagatt caacccaaag
    21841 tgctgacttg tctttttcgt agagcataag cagaccaagt gctgcctccc caggataata
    21901 taacgaagtc catttgtccg acctgccacc ttcagctgga atatatttgg aatagaaact
    21961 tccgtcttct ctttgcatat agacaataaa attgccgagt attctgagat cagaaagggg
    22021 agtaaactcc tgatggaatt tttctatact taagagtgcc acaagcccaa gcccggtgcc
    22081 gcctaatttt acttcaagcg gtcttccact gttattaatt tctggctttg accacactgc
    22141 gagcatttct tttttgcctg gtacttgttc aatagcttta ttttgcaaat atcttccggc
    22201 ttgctctatg gctgtccgta tttctttgtc attaggataa agctcaagat actgagacat
    22261 tgcataaata gtccctgcat gccttaatat attatatttc tgtttaacct cgatagtagg
    22321 gtctgtatta atacggtatt caaacatccc gttttctttc acactctttt gtagatattt
    22381 agcagagagt tgaattgcct tttcgagaga tttagattgt tttgattcag taattgtttt
    22441 tgggacagag caggatgcta caaagaagat taggattaaa taagttatga taccaaaatg
    22501 ataataaaga ccattcatat tatatagagt aaatttaaag aactattcct tgctggcaaa
    22561 tccgacacaa acagactcaa ccacaaacat caagcttagc ccctcatttg gaacactcac
    22621 ttcaacagct ttgcctttaa gttcttttgg ttcatttttt attacctcat tacccttttc
    22681 ttccatcaac ttaataaagg attccgggtc attgattcca cctatatccg ggatttttct
    22741 ttggagggtt tttatctctg cacattcacc gtgtctggac attaaaaacc atttctcaga
    22801 atcatccgaa gccacatatg gaatatatgt ggttagggat aatagactaa acactgttat
    22861 tgcaagttgt tttttcatgt cagatttttt tggtaacaaa caccatatat ttcaataggc
    22921 aacctatata gtatgaacaa gcggaaacta tttttctaca agaatttaga ttacaaatct
    22981 attttatcta agtttaaaat tcttgcagaa tgatataccg ttaatatttc cacaacatct
    23041 gctttaacac gataaataat tctatagttt ccttgtaaga tttcgcggat gtgttcatga
    23101 ttggtttcag caaggattct tcctgattgc ggaaattctt taagtctgtc ggtttaactt
    23161 attattttac ttacaaacaa ccttgcataa tatgcagagt ctcttgatat aaattccgct
    23221 atggcttcga ggtcaccggt ggcctgcaat gtccatctta tttcagccat ttatccagtc
    23281 gcttaattac ctgctcatgc gatatagttt caccattatc agcttgccgg agtcctcttt
    23341 caacttttga cagaagatac aagcgctcca tcgcttcttc aaaactagtg tcatcaggca
    23401 aactctttat tgcatcaacc attacttctt tttctgtttt agctaaagcc attttattac
    23461 ctctatttcc ttataatata agcaatcgca atttaattgt ctactagatt aaagccataa
    23521 cagtttaaga ccacggctct ctataataat aaacaaagtt tgcaaccttg ggtctttctg
    23581 tccagccgag ctttttataa aacccctgtt tataagacgg tttttccttt ccatttgtaa
    23641 gcataagacg catgcatccc ctttctttac cttcttccat cactgtcttt actagcaggc
    23701 ttccggcacc tttacctcca gctcttgggc ttacaaacac gtctgagata tagccttcga
    23761 aactaccaag cataacaaac ggcacccagt gtacagttgt aaatcctatc acttcatctt
    23821 tttcatctgt ggctacatac atagtgtgtc cttcagtatc tttattgcaa tgatcaatca
    23881 acccctcaac tgggcctgat acctcttcta atttcatctg gtttcttctt tcagaccagc
    23941 ctatttccct taatatttcg gccattgaaa ccgcatcttc aactttcgct tttcttactt
    24001 taatttctga catttatatt tctgcctcta taatttattt ttaagtaata tgatcctgtc
    24061 gtaagattca tcaattctgc tttgagatat actaccgtct tttactaacc ttagtatcgt
    24121 gtatatgagg ttttcgcata gattttcttt gtactcttca tcatatgata taattaaaat
    24181 atcgattccg gcattgattg ctaagtttag cgtctgttca aatgtatagt tatctttaat
    24241 tgcacccata tgcagatcat ctgaaaatac tacaccgtta aaacctattt cattccgcaa
    24301 ctcttgatct atatgaatac ttgaaagcgt tgcaggatat ttcggatcaa tattgcggtt
    24361 aaagacatgt gcgctcataa cacagtccac aagattatct ttaacaagct tcttataagg
    24421 aataagttca gactcagacc atgtatcaga aatatctaca aatccatggt gagaatcggc
    24481 ggtagcgctc ccatgaccag gaaaatgttt taaacatgtc agtactcctg cctgcctgtg
    24541 cgctttgata aactctttag aatgtaaagt tacaacttca ggatcacttg aataacttct
    24601 ttctctttta gcaatgatcg gattttcggg attaatttta agatcaacta ctggggcaaa
    24661 atttagattt attcccagcc cacttagagt acctgccatt tccgctgact tttcatatgt
    24721 cagtgatgga tcattcaact caccaagatg ttgctgtgag aaagttttag gaaatccata
    24781 ttcttctttg agtctattaa cctgcccccc ttcatagtct attccaatca atagattatt
    24841 atctgaataa ctctgcaaat tggcggttag attttgcacc tgctcatgtg attcaatatt
    24901 cttaagcctg taatcttttt cttcgagatc aaataatatt actccaccca aattatactt
    24961 gccgatagcc tctgaaatat gatgattctc atcaacttca taaccatcaa agcctatcat
    25021 gagcatctgg gcaatctttt tttctaatga gatatctgtc ataaaaagtg ccgagtctaa
    25081 ttattattta taattcggga ttatgccttt acaccaagta gctgagcaaa aaccagtgtg
    25141 atgacaaata gtaagaccaa tgaaatacca gcagaaagca ttacggcaat aaaagttctc
    25201 tttttctttt ttgtaagaat gacatatgag actaaataaa taagagtgaa agcaagcata
    25261 gggatcgcaa taacaatatc cttttcgcca cctccaaggt ccagagttcc ctggatagtc
    25321 agaaatagca cagctggaaa tacaataagc ggccagatag cgccaattat ttttgcagtc
    25381 atatcttctt tttatctcta tttagttttc ttatataaac tataaagaaa atactgacat
    25441 tcaccaacaa caaaatccca aaggctatgt agattaataa actcctggcg cccagttcag
    25501 ttattatcga tttccctgtg atttcttcgg ctcctaccaa aatcaaattt accaaaataa
    25561 aaattgcaat cggcaaggaa attataaaca aaaaatataa gtaggccttt ttccatgaaa
    25621 cgccctcttt tatatattta gcggtggcat acctaaaaga gagcaaggga cagaccagca
    25681 gagagactat atagagtagt attaatatct ggtacatgaa tatgaataaa atctaaagag
    25741 tagtccagcc cctgtcaatt accaggtctg atcctgtcat atacctggcc tcatcactcg
    25801 atagatatag tactgagtgc gccacctctt ctggctcagc tcttcttatt agaggaatca
    25861 tgtctgctgc gattttatca agtatttcat cagcatcggg agggtttccc ataaattcta
    25921 catccatttt tgtttttatg tatccgggac aaacagaatt gactctaata ttctcctttg
    25981 catgatcaag ggccatactc tttgaaatct gcctaacggc accctttgat gcgttataag
    26041 ccaggcagtc ttccataccc acaataccaa gcacagagga attatttaca atagctcccc
    26101 caccgccttt tgccataatt ggtaccacat acttactcat taaaaataca cccttaacat
    26161 tcgtgtttat cgttctgttc cagatatcat ttgtggttgt aactgctgtt ccagcctcaa
    26221 aaatgccggc gttattaaat agaatatcta tttttttata ttttctttga aagctagcca
    26281 ctaccctttt cacatctttt tcgtttgata catcaccctg aaggacatgg atttcaccct
    26341 ttcccatggc ttcttttttt actttattga ggctggattt tgtcctgccc atgattccaa
    26401 ccttggcacc ctctttacaa aataaaagtg cggtagccct gccaattccg tctccaccac
    26461 ctgtgattag tgctgtcttt ccctttaatt tcatttgttt gtcctcctca aatgagtctt
    26521 aaataatatt aatctctaaa gtttataaac tgaagaggta tttccaaatt tgagtccttt
    26581 aacattgcca tcactgcctg taggtcatcc ctctttttac cgtttaccct aacctgcttt
    26641 tcctgaatac ttgcctgtac cttgagtttg gtgtctttta tcatcttaac catcttctta
    26701 gcaagctcag tctcgattcc atgcctgagt gttacttttt gtcttgctgt gtttgatgac
    26761 agttcgggct catccttatc catgcatctc acatccacct tgcgtgtgct aagctttttt
    26821 gagagtacat ccatcatctg tccaagctga aactcttctt tggtgttgag agttacaata
    26881 ttatccgtta gttcatattt agaatcagta cccttaaaat caaaacgagt ggctacctct
    26941 ttattggcct gatccacagc atttgttact tcatgtaagt caatctctga aactatatca
    27001 aatgatggca tattattttt atcctctata tattaattat ttaacattat tattaacatc
    27061 gtttaactgt ttaacaaaat taataacctc tgggaaaaaa caaaagaagt tatcttccag
    27121 ctcagagtaa ttttgggtta tttcaacaac tgaacctgct agtgagtttt ctcttttaac
    27181 tcttttggac atcctgttaa acacataatc tattccttct atagatttat acatatcaaa
    27241 ccagttctca gacacgagtc ttggcaatat tcctattaac ctttcaggca gaatagattt
    27301 atttttttgt aaaatcgaat agattttatt cacatactgt ggaaaagtct catttgagta
    27361 ttcgtgccag ttttttgcca gataatggtc aaaactaata tctacgataa taccagcaaa
    27421 ccttcttcgt tccttgctta taagcctctt tgatgatctg aatactgaat gcgcatcggt
    27481 ataagcatct atcttgcgat ggatcttaat ccacttaaga atttcacctt cgtaatgctc
    27541 atcgggatta ccttttataa aatcaccaag cagattgccc aagatagact cttcactatt
    27601 gtcagataaa taagtatgag ccaggtagtt cattacagta acttaattta cattttcaat
    27661 aatttcaatc tcaggatgat agcagggtat ccccactcta aaccaaatga acggaaaaag
    27721 gggatttgga agaacataca tccacgccag gttctttatt atatgaccat cttccaggtt
    27781 taaatccatc cagaaataga agaatttata gatgcagaag ccgggaaaat aattaatctt
    27841 aaaaccttct gttagtaaat atttgcggct tatatataaa ttaccctcaa acgaagttat
    27901 cccccagccg cctggcccct cggcaagttt tgtgttagtt ggtttatgat atacagaaat
    27961 tattttctta gacataggca gagacatagg cagacaaatt ctaaaaaaag aattttctgg
    28021 aaatccaggt tttacactga taccactggc catttttttt ctgaaatgtt tgcatataaa
    28081 agttcttgtc ggttcttgtt tcaattggag ggttaaaatt cttcacttct tcttcagtaa
    28141 taatcacaca aacagggcgg aagaacagat agaagactaa taccattaac actataggta
    28201 ctgtaatcca taaaaatctt ttcatttccc cacactctga ctataggcca ttgcagacga
    28261 aaatgctgat actgtatacg gcacaaaaaa tgtgattatg gattttaagt aaaataccca
    28321 atggaagtcc ctgttcataa tcgtatcaaa atgattaatc aaaattagta ttggagcgac
    28381 tacaaatgcc accttaagtg cccttagtgt tgtatccttt ctaaagaagt ttttaaaaaa
    28441 tattgataga gtcatgaggt ttatactata taggattacc ctggaattga taaattagaa
    28501 tctctggtca tctaccccga ttctgagacc gaatacacaa gcgtgtcggg cctgaatgtg
    28561 taccaggcaa accagtatgt aacaaaagat tttataggcg atccattgtt atcataagca
    28621 gaaacaaatt tgccgtcatt atattttatt gtaattttgt cactgccgac ttgatccttt
    28681 agaggtgtgc tcaccttgcc aagctcttta aaagcatagg ccttggcaac gccgtcagaa
    28741 gaaattacca caagcacctt ctcttttgga tgatatcttt tatcctgatt atctacaggg
    28801 aaatataaac ttgttgattt ttcataaccg ccataaggca tattactata atccctgtta
    28861 aatcccgtat ctcttgaaag cacaagggta ttcggatgtt ttttcttcca ctcaccccag
    28921 gtagcaagca ccgacggaag aacccccatc tcggtcccgg cttttggccc cgagattgcc
    28981 tccatttcca gctgtgacca caggctctga ttctgatgat catagaacaa aacatcagac
    29041 tggtaaagaa gtccagagat tccgaatgtg agctcctgtc cgtctatgtt tctgtcaaaa
    29101 acgagggttg atccgcaaag cggacagaat gaaaccatga tcggtttact gcctacttta
    29161 tcgtttacgg cttcatgcca cactaatatt ctaagaggat aagccttaaa tactccttcc
    29221 atttctactc ctgaaaccaa gtcagaactt tttagccatg aagcactgga tgcagattca
    29281 aatttaggat ttaatattgc tggaataccg tcttttggag gcccccctga aaaaatcttt
    29341 tcttttggta taagactgtt tgaaacatca aagccattgt aatcttcaaa caagcttatt
    29401 gaataagagt tataagttgt aagaagtaaa attattactg ataagataaa tatatttcgc
    29461 ataattaagt atgatgaatg caattgtaat aagattctga ttgcccaccg actaaccaat
    29521 cttcttccac tcatagaaat aatctgagac tttgtctctt atgtataatc tggagcgatg
    29581 gattctggat tttgtggctg gtacagatat atctaaaact tcggcaacct cttcatttgt
    29641 aagcccttct atatcacgaa gataaaatac aaccctgtaa acctcgggaa gttcatctac
    29701 cgctttttct aaaattttga gcgcctcttt tacatatata acaatatcgg gtcttgagct
    29761 ccagtccttg tggtgcatcc tgcccataag ggtgccttta ttatcatatg gtacataatc
    29821 ctctaagctc aggtcgtact catatttttt ttcagaccgc agcatcatat agcttgcatt
    29881 agcagttact ctgtataacc agcttgagaa tttcgattct cccctgtatg tatggagctt
    29941 ctttataagc gtatagggca taattgcaaa aaatgtgtgc tgaaaatggg gttttagcct
    30001 ttatttacta tattataatg agaaaaaagt taaacaggtt taactatgaa caaaaaactg
    30061 tcccctttgc agtgcacaaa aa
//
LOCUS       DBNGSV010000195         3481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_727141, whole genome shotgun sequence.
ACCESSION   DBNGSV010000195 DBNGSV010000000
VERSION     DBNGSV010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_727141"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1101)
                     /locus_tag="AC159U_07115"
     CDS             complement(106..1101)
                     /locus_tag="AC159U_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336866.1"
                     /translation="MNESTGYWKGVWIEFKKDRLSVIALCVIITLFIVALFDDLIAGS
                     KPIIIKYDGSLYSPAVFNHKDLIGVDLSDSDLVKDKASFVIYPPVKYSPTENDLLSVL
                     SPPDSNHYFGTDDRGRDVLSRMIHGAKISLSIGFVAVGIAFIIGIIMGGIAGYYGGKT
                     DFIISRLFEIMITFPVFFLILTILAFRDPSIYNIMIVIGATGWTGIARLIRGDFLKIR
                     NYDYITAQKALGSGDIRIMFMHILPNALAPVLVAATFGIAGAILTESALSFLGFGVPP
                     PTPSWGDVLSQSKKYVDFAWWLVIFPGLAIFLTVTSFNLVGEGFRNALDPKLRGR"
     gene            complement(1103..2104)
                     /locus_tag="AC159U_07120"
     CDS             complement(1103..2104)
                     /locus_tag="AC159U_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336867.1"
                     /translation="MKSYIVKRLLLAIPTLIGITLITFCIIQLAPGNPVERNQQLEQG
                     IQSEEITKEIVEQTKKLYGLDKPIHIQYLIWLKRIVTFDFGDSYKDHRPVLDKIKERL
                     PITLTLNLISIFLVYIIAIPIGIMSAIKDRTIMDRITTFLLFGLYSIPSFWLAMMLIY
                     FLGGGEHWDIFPIYGIQSANSDGFSIFQKFTDFLWHITLPVICLTYADFASLSRYQKG
                     SLLEVLRENYVRTARAKGLEEKTVITKHVLRNSLIPIITIVSAILPAMIGGSVIIESI
                     FSIPGIGQLGFEAILSRDYPLIMAITTISAFLTLIGILIADILYVLVDPRITFEGRS"
     gene            complement(2138..>3481)
                     /locus_tag="AC159U_07125"
     CDS             complement(2138..>3481)
                     /locus_tag="AC159U_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HIR3336868.1"
                     /translation="ISDDKLTFTFKLRDDVFWHDGKPFTSEDIVFSYNTIMDPKVDSA
                     QLKGYYQEIKKVEAIDKHTVKFTYARPYFLALEFCGGIPIVPKHIFDKGDFNKNPAGR
                     HPIGTGPYKFSKWETGREIILDKNEDYWGESPKLQKLVFKIINDSNVAFQVLRKGELD
                     LAGLTPIQWEKQSKTKKFEDIFDKYSYFRPNYSYIGWNIDRPFFSDKRVRTAMSHLVN
                     KELILQKILYNLGATVTNPFYIKSKEYDKTIKPIKYSPESAIALLNEAGWIDTNSDGI
                     RDKDGVKFEFEFLVPNGSDTSEKISTILKEEMDKAGIRMNIRKIEWAVFVQRLNERKF
                     DAVTLSWSMGVETDPYQIWHSSQKSSGGSNFVGFDNAEADRLIEEARQEFDSEKRTKL
                     YKKFANIIHDEQPYTFLFCGKSPIAVNKRFENVNVYPLGVDFLEWYVPAELQKYH"
BASE COUNT         1088 a          673 c          556 g         1164 t
ORIGIN      
        1 tggtacggct atagatgcat tacttaccac ttcaaagccg gtttctttat ctccaataga
       61 gatagtgaga ttatttattt ctaatatgtt ttccatttaa attatctatc ttcctctgag
      121 tttaggatca agagcatttc taaatccctc accaaccaga ttaaatgagg ttactgttaa
      181 aaaaatagcc agtccaggaa aaattactaa ccaccaggca aaatcaacat attttttaga
      241 ctgagacagc acatctcccc agcttggcgt tggtggtggc actccaaatc caaggaagct
      301 aagggcagat tcagttaaga ttgctcctgc aattccaaaa gtagctgcta ctaaaaccgg
      361 cgcaagcgca tttggaagta tatgcatgaa cattatcctt atatcgccag aaccaagtgc
      421 cttttgtgca gtaatataat cataatttct aatctttaaa aaatctcccc ttataagtct
      481 tgctattcct gtccagcctg tagctcctat gactatcata atattataaa tactggggtc
      541 tctgaaagct agaatagtta aaataagaaa gaatacggga aatgtaatca ttatctcgaa
      601 tagtcttgaa ataataaaat cagtctttcc tccgtaatac ccggcaattc ctcccattat
      661 tatccctata ataaaagcaa taccgactgc aacaaaccct attgaaagcg atatttttgc
      721 accatgaatc attctgctga gtacatcccg gcctctgtcg tccgttccga aataatgatt
      781 gctatcaggc ggagagagta cagaaagaag atcattttcc gtagggctat atttcaccgg
      841 aggatatatt acaaatgagg ctttgtcctt aacaagatca ctgtccgaca agtctactcc
      901 tataagatct ttatgattaa aaacagcagg ggaatataag ctgccatcat attttatgat
      961 aataggcttg cttccagcaa taaggtcatc aaacagagca acaataaaga gcgttattat
     1021 tacgcaaaga gcaatcacag atagtctgtc ttttttaaac tctatccata cccctttcca
     1081 atatcctgtt gattcgttca atttaacttc ttccctcaaa cgtaattcgc ggatctacaa
     1141 gtacatatag tatgtcggct attaatattc caataagagt taaaaatgct gaaatagtag
     1201 taatagccat gataagcgga taatccctgg atagaatcgc ctcaaatccc agctgtccaa
     1261 taccgggaat ggaaaatatg ctctcaatga tgacactacc accaatcatt gccggaagta
     1321 ttgcagatac aatggtaatt attggtatca atgaatttct cagcacatgt ttcgttatca
     1381 cagttttttc ttcaagacct tttgcccttg cagttcttac ataattttct cttaggactt
     1441 caagcagact tcctttctgg tatctggaaa gtgatgcgaa atctgcatat gttaggcaaa
     1501 ttacgggaag tgttatgtgc cacagaaagt ctgtaaattt ctgaaatatg gaaaagccat
     1561 cagaatttgc agattgtatt ccgtaaatag gaaatatatc ccagtgctcc cctcctccca
     1621 ggaaatatat aagcatcata gcaagccaga agcttggaat tgaatatagg ccaaatagta
     1681 aaaacgtagt tatcctgtcc attatagtac ggtctttgat tgcggacata attcctatag
     1741 gtatagcaat aatataaaca agaaatatcg aaatcaggtt taatgtaagt gtgataggca
     1801 gtctttcttt aatcttatca agtaccggtc tgtgatcttt ataagaatca ccgaagtcaa
     1861 aagtgactat tcgcttaagc catattaagt actgtatatg aattggctta tccagaccgt
     1921 atagcttttt agtctgctca acaatttctt ttgttatttc ttcggattga atgccttgtt
     1981 caagctgttg gtttctttca acagggtttc caggtgcaag ttgaataata caaaatgtaa
     2041 ttaatgttat ccctattaga gtgggaatag ctaaaaggag tctcttaact atataggatt
     2101 tcattatggc aatagttgta atatatgttt aacaagttta atgatatttt tgcagctctg
     2161 caggtacata ccattcaaga aaatctactc ctagtggata tacattaaca ttttcaaagc
     2221 gtttatttac cgcaataggt gattttccac aaaacaagaa tgtatatggt tgttcatcat
     2281 gaattatatt tgcaaatttt ttatataatt tggttctttt ttcactatca aattcttgtc
     2341 gtgcttcttc tataagtcga tcggcttcgg cattatcaaa acccacgaaa tttgaaccac
     2401 cacttgattt ttgtgaggag tgccatattt gataagggtc tgtttctaca cccattgacc
     2461 agctgagagt aacggcatca aatttccttt catttaacct ctgaacaaat acagcccact
     2521 ctatttttct gatattcatt ctaattccag ctttatccat ctcttctttt aaaatagttg
     2581 atattttttc gctagtatcc gatccattag gtacaagaaa ttcaaactca aacttaacac
     2641 cgtctttatc ccttatacca tctgagttgg tatcaatcca acctgcttcg tttagcaacg
     2701 caattgctga ctcagggctg tattttatgg gttttatagt tttatcgtat tccttactct
     2761 taatataaaa aggatttgta accgtagctc ctaaattgta gagaatcttc tgtagtatta
     2821 attctttatt aactaaatgg ctcatcgcag ttcttactct tttatcagag aagaatggcc
     2881 tatctatatt ccagccgata taactataat tgggtctaaa ataagaatat ttatcaaata
     2941 tatcttcaaa tttctttgtt ttactttgtt tttcccattg tataggagta agacctgcaa
     3001 gatctaattc accttttctt aagacctgga atgctacgtt actatcatta ataattttga
     3061 ataccaattt ttgcagcttt ggactttctc cccaataatc ttcattttta tctaatatta
     3121 tctctcttcc agtttcccat ttagaaaatt tatatgggcc tgtaccaatt ggatgcctac
     3181 cagcgggatt tttattaaaa tcacctttat caaagatatg cttgggtaca ataggaattc
     3241 caccgcaaaa ttctagtgct aaaaaatatg gccttgcata agtaaattta acagtatgct
     3301 tgtctattgc ttcaactttt ttaatttcct ggtaataacc cttaagttga gctgaatcga
     3361 ccttgggatc cataatagtg ttatatgaaa agacaatatc ctctgaagta aatggcttac
     3421 cgtcatgcca gaagacatca tcccttaatt taaaagtaaa tgtaagctta tcatccgata
     3481 t
//
LOCUS       DBNGSV010000196         1294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1004980, whole genome shotgun sequence.
ACCESSION   DBNGSV010000196 DBNGSV010000000
VERSION     DBNGSV010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1004980"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC159U_07130"
     CDS             <1..261
                     /locus_tag="AC159U_07130"
                     /inference="COORDINATES: protein motif:HMM:NF020996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336869.1"
                     /translation="TLNPDSIFLDPPWGGTDYGNIGKFKLLNFEPDGSKLLDLCFGIT
                     DKIVIRLPKNFDLEELKQFNRKFDLQENILEAQLLHYCVYFR"
     gene            318..605
                     /locus_tag="AC159U_07135"
     CDS             318..605
                     /locus_tag="AC159U_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336870.1"
                     /translation="MTTCPHCSAQCNPLKFLKVTKWTPYVCDKCTGKSDFPQKENIIL
                     GVVTGIIFFILAYLLSKVIGLFGLIPAVILTIVLIPIYQYYFMYLEKIDDR"
     gene            638..1009
                     /locus_tag="AC159U_07140"
     CDS             638..1009
                     /locus_tag="AC159U_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3336871.1"
                     /translation="MSLKVNSKEKQPGAFVISCDGSLDSNTYSSLENRVALIFEGTPK
                     LIVFDMENLNYISSMGVRVILQTQKKMKSLGGSSRLMNLKPQVEKVFEIINALPSDQV
                     FSSEQEMDAYLDKIQKRVSEE"
     gene            complement(1022..>1294)
                     /locus_tag="AC159U_07145"
     CDS             complement(1022..>1294)
                     /locus_tag="AC159U_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336872.1"
                     /translation="LKWHKKPLRANRINYKAEFYFHFSGDTCRVLASGPVGYSFNSKG
                     ELIGWTEDSGDFHIPKFIYSKDAIWERTTVGWVKDYIRKLENSKNN"
BASE COUNT          414 a          214 c          231 g          435 t
ORIGIN      
        1 accttaaacc ctgattcaat tttcttggat cctccctggg gtggtacgga ttatgggaat
       61 atcggcaagt ttaaactatt aaatttcgag cctgacggaa gtaaattact tgatttatgt
      121 tttggcataa cggataagat tgtaattaga cttccaaaga actttgatct ggaagaatta
      181 aagcaattta acagaaagtt tgatctacag gaaaacattc tggaagcaca gttgttacat
      241 tattgtgtat acttcagata gtctggcgca ttttatattt tattattttt gttaattgtg
      301 ttaaatttca attcttaatg acaacctgtc cgcactgcag tgctcaatgt aatccgctta
      361 aatttcttaa agtcacaaaa tggactccat atgtctgtga taaatgtacg gggaagtcgg
      421 attttcctca aaaagaaaat attattttgg gcgtggttac agggattata ttttttatac
      481 tggcgtatct gttgtcaaaa gtgataggtc tttttggact cataccggca gtaattttaa
      541 cgattgtttt aattccaatc tatcagtact atttcatgta tcttgaaaaa atagacgaca
      601 ggtagatttt ttgtcctaac caacttataa tctaattatg tctttaaaag taaactctaa
      661 agaaaaacag ccgggagcat ttgttatttc ctgcgatggc tctcttgata gcaataccta
      721 ttcttctctt gaaaacaggg tggcattgat atttgaaggt actcctaaat taatagtctt
      781 cgatatggaa aacctgaatt acataagcag tatgggcgtg agggtaattt tgcaaacgca
      841 gaagaaaatg aaaagccttg gcggatcttc caggctcatg aacctaaagc cacaggttga
      901 aaaagtgttt gaaataataa atgccctgcc ttccgatcag gttttttcat cagagcagga
      961 gatggatgcg tatctggata aaattcaaaa aagagtatca gaagagtaat ttattattaa
     1021 attaattatt tttcgagttt tctaattttc taatataatc ttttacccaa ccaacagtag
     1081 ttctttccca tatagcatcc ttagaatata tgaattttgg aatatgaaaa tctcctgaat
     1141 cttcagtcca tccaattaat tcccctttag aattgaatga gtaacctaca ggtccggatg
     1201 ccaaaacccg acatgtatca ccagaaaaat gaaaatagaa ttcagcttta tagtttatcc
     1261 gatttgctcg tagaggcttc ttatgccact ttaa
//
LOCUS       DBNGSV010000197         5938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_935891, whole genome shotgun sequence.
ACCESSION   DBNGSV010000197 DBNGSV010000000
VERSION     DBNGSV010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_935891"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1299)
                     /locus_tag="AC159U_07150"
     CDS             complement(136..1299)
                     /locus_tag="AC159U_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336873.1"
                     /translation="MNPKGEIDLANVDTWHGNGELVDFNLASLEQANLPVGTPVKVIC
                     VRPETRKSDKEEQNSENKEAASNTSSKENNGWEALVRPLDTFYPEQFGEKSLIQLDVE
                     AKSDLHPEGFVNIKYSESEGENSNDINDLLNRSVENWLALVVDEDLRFEKGTEDNRYI
                     LEGDDLKVICLRSIFLVKPSYLVNNEHLKLVGRTVKTATQLSPKGKVKIDGKIFDGGL
                     VDYNLQVKKNVVTPKDTELVIVGVSGYDMTLLLQRPEEVLKETRFIGSVVKAEDVLNP
                     YGNVILDSKQWASSVVTDSLTPFYGIEIEKDQNLTIVAVATQLLVVESDLLVPPFWRD
                     KWVPILTLLTAGAATGIILPLALDDGGGSGPDAPPPPGDDDDDDDDVASPVIP"
     gene            complement(1342..1929)
                     /locus_tag="AC159U_07155"
     CDS             complement(1342..1929)
                     /locus_tag="AC159U_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336874.1"
                     /translation="MINQTYAAAMIGKLYVYNINPKNPQVHPIFEGDRIKQGNKYVLR
                     LNGATMVADSNTDVQILDEHGVKIVKIASGIIRFRVSPQKIRISFRTPQGEVFTPEVI
                     KASTNIIDGQITVKDNTIVELNEGTLEALTLNGVTKIESGEGVLLTQANIAQSDIEQA
                     SENSTPPASNAAKEQITTNKNLVAVRDKEIPENIR"
     gene            2515..3567
                     /locus_tag="AC159U_07160"
     CDS             2515..3567
                     /locus_tag="AC159U_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3336875.1"
                     /translation="MLVLSYFLFGFFLSRYITNKSKLFIVIIPIILLAVIETAIGFYQ
                     SFIVYGITNSKSAHGTFVNKNHYAGLLELLIPILIGLGLSFNKNWGSKSKISLSSLLN
                     ADRFKQLIILNIVAATFIALIISKSRMGIMSIVISLIFFFFLPSFIAKNYKNFIWFIV
                     ISVLFFILLIFLIDITPVLDRFSELSAAARIKVWKDCIGIITDFHLFGSGLGTFKYIY
                     PLYKENLKTAVDYNYAHNDYIYLLVETGFIGFSCLTAGLIILFKDSIKYLRFHLKTSN
                     PFGFFITLGALCSVVSILIHSLADFNLHIPANALIFTAMIGIIYGINSKNNSKDIVKV
                     KRVRKTKIGEPINSEV"
     gene            complement(3551..4621)
                     /locus_tag="AC159U_07165"
     CDS             complement(3551..4621)
                     /locus_tag="AC159U_07165"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF026257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3336876.1"
                     /translation="MECIETLDFKSVDWTYIINFSKVHGITSLICFNINKHKNISVPE
                     LELKKLKTASLQNSKKNLFLTSVLLKILKDLKKIGIEAVAHKGPVLSRMLYDDITIRD
                     FTDIDIIVYKKDVIKAKEYLIQNGYSPQFDLKGYKEDYYLNSKYYYINFLRDDSKAAL
                     DLHWALSAPNYGFSTGLEHFSSKLDKINLNNVSLDVLREEDLFLQLCIHGAKHNFSRF
                     CWLADIVNLAAVNKGMDYEYITRQAAKLGCTNIVTFSAELIWCLFNVRLHVCHDLKPE
                     QKTLLFAKNLVVNNTGHSAGSLFPVLFDTAKDRIIYYLNTYIYPTPIEFSVVNLNKYL
                     FFVYYIIRLIRMFYKGIAKLLN"
     gene            complement(4807..5814)
                     /gene="trpS"
                     /locus_tag="AC159U_07170"
     CDS             complement(4807..5814)
                     /gene="trpS"
                     /locus_tag="AC159U_07170"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3336877.1"
                     /translation="MSDKKDVLVTGDRPTGRLHLGHLVGSLNNRIKFQQEYNCYFLIA
                     DLHMLTTHFDKVKEVEESVIEMVIDWLSVGMDPNKSTFYLQSEIPYITELYTILAMLC
                     SVARSERIPTLKEKIQDMGVGENYSVGLLGYPILMAADILMYKAVKVPVGEDQLSHIE
                     ITRELARRFNHIYGDYFTEPEPIISETSRLVGTDGDRKMSKSLDNCIYLSDDPKAVEK
                     RVRGMFTDPNRIRADIPGKVEGNPVFIYHDAFNDNTEEVDDLKDRYRTGNVGDVEVKK
                     KLAAAINKLLEPIREKRSYYETRRDEVKDIIKQGTAKAKEDAGVIMNEILDHIGMYRP
                     L"
     gene            complement(5866..5938)
                     /locus_tag="AC159U_07175"
     tRNA            complement(5866..5938)
                     /locus_tag="AC159U_07175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5903..5905),aa:Arg,seq:acg)
BASE COUNT         1809 a         1192 c          909 g         2028 t
ORIGIN      
        1 taaagcaaaa gctatctgtt gttctgtaaa tcttgacttc ctcatggcaa aatacctcct
       61 gatttattac gttattttgc cagatttctc tacttttgac tggttcagtt tacggggtga
      121 aggtcaatta taactttaag gaattacagg acttgcaaca tcatcatcat cgtcatcatc
      181 atcgccgggt ggtggaggtg catcaggacc gcttccacct ccgtcatcga gtgcgagcgg
      241 aagaattata cccgtagcag cgcctgctgt taataatgtt agtattggca cccacttgtc
      301 tctccaaaat ggcggaacca aaagatccga ttcaacaacc agtagctggg tagcaactgc
      361 aactattgtg agattctgat ctttttcaat ctctatacca taaaatgggg tgaggctgtc
      421 tgttacaacc gatgaagccc actgtttact atcaagtatc acatttccat atggatttag
      481 cacatcctct gcttttacaa cagacccaat aaaccttgtt tctttaagaa cttcttctgg
      541 tctttgaagt aaaagagtca tgtcataccc actaacacca actataacaa gctcagtatc
      601 ttttggagtt actacatttt ttttaacctg aagattataa tctacaagcc ctccatcaaa
      661 tatctttccg tcaatcttta ccttaccttt gggacttagt tgggtagcag tttttactgt
      721 ccttcccact aattttaaat gctcattatt taccagatac gacggcttta ccaaaaatat
      781 tgacctaaga catataacct taaggtcgtc accttcaaga atataacgat tatcttctgt
      841 acctttttca aaccttaaat cctcatcaac caccagagca agccagtttt caacactcct
      901 gtttaataaa tcatttatat cgttactgtt ttcaccttca ctctcactat acttaatatt
      961 aacaaaaccc tccgggtgaa gatcactctt tgcttccaca tctaactgaa taagactctt
     1021 ttctccaaac tgctcaggat aaaatgtatc cagaggtctt accaatgcct cccagccatt
     1081 attctctttg ctgctcgtat tactcgcggc ttccttattt tcactattct gctcttcttt
     1141 atcagattta cgtgtttctg gtcttacgca aattaccttt accggagttc ctaccggcag
     1201 atttgcctgt tccagagatg caagattaaa atcaacaagt tctccgttgc catgccaggt
     1261 atctacgtta gcaagatcaa tctcaccttt aggattcaaa atagattctg ctgatccttt
     1321 ttctccaatc agctcgagta tttatcgtat attttcagga atttccttgt cacgtactgc
     1381 aaccaggttt ttatttgtgg ttatttgttc ttttgcagca tttgaagcag gaggtgttga
     1441 attttcagac gcttgttcaa tatcggattg agcaatattt gcctgggtca acagtacacc
     1501 ctcacccgat tcaatttttg taactccatt tagagtaaga gcttcaagcg taccctcatt
     1561 aagctcaacg atagtattat ctttaaccgt gatctgtccg tctattatat ttgtgctggc
     1621 ttttataacc tctggcgtaa atacttcacc ctgaggagta cgaaatgata ttctaatctt
     1681 ctgagggctt actctgaatc taataattcc acttgcaatt ttaactattt ttacaccatg
     1741 ttcatccaaa atctgaacat cagtattaga atcagccacc attgtcgcac catttaagcg
     1801 aagcacatat ttattgccct gcttaattct atcgccttca aagattggat gtacttgtgg
     1861 atttttaggg ttaatattgt aaacatacaa tttaccaatc atagccgcag catatgtttg
     1921 gttaatcata aacaacataa caaataaaaa atagataaat ctcttaaaca tcatggcaac
     1981 tcccgcatat tcatataaat atgattaact catcacttat gttatatttt cacaaacaag
     2041 tgcaataatt tcaaattata tcattattct aataatatta acagttaatt aacaaaaaat
     2101 ataaacctaa aatattatta acctaaggta gtattgatta tcaatttctt tattattatt
     2161 ttcacttcat cgttaattct attcccaccg ctttattatg gcagtgtact gacgctctca
     2221 atacaactaa tacagttagc tatatatata attatggctc tatggttaac aaaacattat
     2281 tttgagaaac aaagtataaa gataaattta gtaagattat taccacttat catccttgta
     2341 cttctatgtt tttttcaaac tataaacctc ccaaattggg tacataatat actcagcagc
     2401 aaaagctttg agatatggga caatagtcgc aacatactaa gtaccattag tccatattac
     2461 gacagggatt ggtttacgct cagcatatac ccgtttgcga cgaaaaactc actcatgctg
     2521 gttttatctt attttctttt tggctttttc ctgtcgagat atattacaaa taagtcaaag
     2581 ctgttcatag ttattatacc aataatatta ttagcagtca ttgaaacagc tataggtttt
     2641 tatcaatcat ttatagtata tggaattacc aacagcaaat cggcgcatgg aacttttgta
     2701 aacaaaaacc attatgcagg tttacttgaa cttttaattc ctatattaat aggactcggg
     2761 ctatcattca ataaaaactg gggtagcaaa agcaaaatct cactcagttc gctcttaaat
     2821 gctgaccgtt ttaaacaatt aataatttta aatatagttg cagctacttt tattgcacta
     2881 attatatcaa aatcccgcat ggggataatg agtattgtta tttcactaat attctttttc
     2941 tttcttccga gctttatcgc caaaaattac aaaaacttca tttggttcat agtaatctcg
     3001 gtactttttt ttattttatt aatatttctt atagatataa caccggtctt agatagattt
     3061 agcgaactaa gcgctgcggc aagaataaag gtttggaaag attgcattgg aattattact
     3121 gattttcatc tttttggatc agggcttgga acatttaagt atatttatcc actatacaag
     3181 gaaaacttaa aaacagctgt cgactacaac tatgcccata acgattatat atatttactc
     3241 gttgaaacag gctttattgg atttagctgt cttacggctg ggcttataat tttgtttaaa
     3301 gattctataa agtatctcag attccattta aaaacttcta acccatttgg tttttttatc
     3361 acccttggag cactttgctc agtagtgtca atactaattc acagccttgc ggactttaat
     3421 ctgcatattc cagccaacgc tctaattttt acagcaatga ttgggattat ttatgggatt
     3481 aactctaaaa acaacagtaa agatatagta aaggtaaaga gggtgagaaa aaccaagatc
     3541 ggtgaaccta ttaattcaga agtttagcaa tgcccttata aaacattcga atgagtcgaa
     3601 ttatataata cacgaagaat aaatatttat tcagattaac aacagaaaat tctattggtg
     3661 taggataaat atatgtattt aaataatata ttattctatc ttttgctgta tcaaataaaa
     3721 cagggaacaa cgaacctgca gaatgaccag tattatttac aacaaggttt ttagcaaaca
     3781 ataaggtttt ttgctccggt tttaaatcat ggcatacatg aagccgcaca ttaaataaac
     3841 accatattaa ttcagcgcta aatgtaacaa tgttagtaca tcccaacttt gcagcctgcc
     3901 gggtaatata ttcataatcc atacccttgt taacagctgc aaggtttact atatcagcaa
     3961 gccagcaaaa tcttgaaaag ttgtgttttg cgccatgaat acaaagctgc aggaataaat
     4021 cttcttccct aagcacatcc agagacacat tattaagatt tatcttgtcc agttttgaag
     4081 aaaaatgttc aaggcctgta gaaaatccat aatttggagc cgatagtgcc cagtgtagat
     4141 caagtgccgc cttgctgtca tctcttagaa aatttatata gtaatattta ctgtttaaat
     4201 aataatcctc cttataacct ttcaaatcaa actggggaga ataaccgttt tggataaggt
     4261 attcttttgc ctttatgaca tcttttttgt atacgattat gtcaatatcc gtaaaatctc
     4321 taatagtaat atcatcatag agcatccttg ataacactgg ccctttatga gcaacagcct
     4381 caattccaat cttttttaaa tcctttagga tctttaacaa cactgatgtt aaaaaaaggt
     4441 ttttcttgga attttgcaga cttgcagttt taagcttttt aagctctagt tcaggtacag
     4501 aaatattttt atgcttatta atattaaaac aaatcagtga agttattcca tgtacttttg
     4561 aaaagttaat tatataagtc cagtcaacag acttaaaatc tagtgtttca atgcattcca
     4621 aatcacttgg atccgggcta gctttactta aaataattaa aagtttttgc tcctccgtca
     4681 gtgactcaaa aatatgggca ttattatagt tcaaattagt tatccataac tagtttgtac
     4741 ctataataac agagttgaaa caacctaaat agaactattt tgataaataa ggaatttttt
     4801 aaaaaactac agaggtctgt acattccaat atggtcgaga atttcgttca ttataacacc
     4861 tgcatcctct ttggctttag ctgtaccttg ttttattatg tcttttactt cgtctcttct
     4921 ggtctcgtag taacttctct tttctcttat gggttcgagt agtttattta ttgctgcagc
     4981 aagcttcttc tttacttcta cgtccccaac gtttccggtt ctgtatctgt ccttaaggtc
     5041 gtctacctct tcggtattat cattaaaagc gtcatgatag ataaagacag ggtttccctc
     5101 aactttaccg ggaatatccg cccttatgcg gtttggatca gtaaacattc ctcttaccct
     5161 tttttcaact gcctttggat catcggacag ataaatacag ttatcaaggc tcttgctcat
     5221 ttttctgtca ccatcagtac ctacaagcct tgaggtttcg cttataattg gttctggctc
     5281 agtaaaataa tctccataaa tgtggttgaa tctccttgca agctcccttg taatttcaat
     5341 gtggcttagc tgatcttctc ctacgggaac ctttacggct ttatacatta atatatcagc
     5401 agccattaat ataggatatc caagaagtcc caccgaatag ttttctccca ctcccatatc
     5461 ctgaattttc tcttttaggg ttggaattct ctcagaacgc gccactgagc aaagcatcgc
     5521 aagtattgta taaagctcag taatataagg tatctccgac tgaaggtaga atgtagattt
     5581 gtttggatcc atcccgacac taagccagtc tatgaccatt tctatcacgc tttcttccac
     5641 ttcctttacc ttatcgaagt gagttgtaag catgtgtagg tctgctatga ggaaataaca
     5701 gttatattcc tgctggaact taattctatt gtttaaagag ccaaccaaat gaccaaggtg
     5761 aagtcttccg gtaggacggt cacctgtaac aagcacatct tttttatcac tcatattctt
     5821 ttagctccta acaataaata gcaattaatt aaatatataa agacccgcgc ccggagggag
     5881 tcgaaccccc aaccttgtga tccgtagtca cacgctctgt ccagttgagc tacgggcg
//
LOCUS       DBNGSV010000198         2635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_936016, whole genome shotgun sequence.
ACCESSION   DBNGSV010000198 DBNGSV010000000
VERSION     DBNGSV010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_936016"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="AC159U_07180"
     CDS             <1..1241
                     /locus_tag="AC159U_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336878.1"
                     /translation="SRRFGLGMEIDRGPLTYKSKHEPVSLSEVEEAILVWTGLGIKGI
                     NLSDFPPHVGLDLEMQFTSKTIPALGDVHRTELFYTNDEGTYMIKMHDKRPDDFKGLE
                     GLTRDKRIDRIVELFRESKIKIHDGRADLPSKPPGIAAHNLWNVNKPGTSVFMPVTDL
                     SACIINLLFFYMRPDHRFNFVDELHGMRPPGTKGWLEKGFLDESKRMPLIEAELRFAN
                     GYIAEQAFMGQNMVLALQTLGLGGWLFSGFASMFMLGGTPFNRGLGFRFITPDIKGDT
                     GNPNPVAVGKDDMFHAFCPPYYKDMGEAVEALNDLKWTNWETHKMPYKDPEGVLGEIE
                     RPSKEEIQVVKDICNYIYDTYGRFPAFSDPMFLRFMVQAHHLDLDFYDKYYPEGAYTE
                     NCKHHFGRWHPEIDDPFANK"
     gene            complement(1276..2130)
                     /locus_tag="AC159U_07185"
     CDS             complement(1276..2130)
                     /locus_tag="AC159U_07185"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3336879.1"
                     /translation="MDINATTKLYGIFGHPVSHSFSPSMHNAAFEHLGLNCVYLAFDI
                     LPENLSTAVESIRQLNVQGVNVTIPHKQEIMNYLDDISDAAKYTGAVNTVHNKNGKLF
                     GHNTDVGGFLKDLEIELGITEFSDVSACLIGAGGAARAVLSGLCMKGIKKIVIANRTI
                     SKASDLAQYFSKQFSDTVISSVDLGDEQAVSKHLSESNLLINSSSAGMTGHEELNIAL
                     DSLHKNSSVYDLVYNPKETRLVKDARSLGQNACTGLGMLLYQGAESFELWTGKKAPID
                     IMKQALGL"
     gene            complement(2138..>2635)
                     /locus_tag="AC159U_07190"
     CDS             complement(2138..>2635)
                     /locus_tag="AC159U_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HIR3336880.1"
                     /translation="GFKATIIDPVTNGSNVISSTAIRNLILDGQISRANKYLGRNFSL
                     EGTVTEGEKRGRVLGFPTANLKTDWELLPKPGVYITLTHLGKNTHRSITNIGFRPTFG
                     KNSLLIESHIFNFSSEIYGDEIRIEFLSRLRDEKKFGGLEELKQAISQDVKTANQYFD
                     DNLVT"
BASE COUNT          750 a          510 c          574 g          801 t
ORIGIN      
        1 gatccagaag attcggtctt ggaatggaga ttgacagagg gccgcttaca tataaatcaa
       61 aacatgaacc tgtatctctc tccgaagtag aagaagcaat actggtgtgg acgggtttag
      121 gaataaaagg tattaatctt tctgatttcc caccgcatgt gggccttgat cttgagatgc
      181 agtttacaag taaaactatc ccggcacttg gtgatgtgca cagaactgag cttttttata
      241 caaacgatga aggcacatac atgattaaga tgcatgataa gagaccggac gattttaagg
      301 gtcttgaggg gcttacacga gacaaaagaa tcgatagaat cgtagaacta ttcagagaat
      361 caaaaataaa gattcatgac ggaagggcag atcttccaag caaaccccca gggattgccg
      421 cacacaatct atggaatgta aataagccgg gaactagtgt atttatgcct gtcacagacc
      481 tttctgcatg tattattaac cttctgttct tttatatgcg ccccgatcac aggtttaatt
      541 ttgttgatga gctccatggg atgagaccac caggaacaaa gggctggctg gaaaaaggat
      601 ttttggatga aagcaagaga atgcctctca ttgaagcaga gctaagattt gcaaatggat
      661 atatagcaga gcaggcattt atgggtcaaa acatggtgct tgcgcttcag accctggggt
      721 taggcgggtg gctttttagc ggctttgcga gcatgtttat gctcggaggt actccattta
      781 acagaggtct cgggtttaga tttattactc ctgatattaa aggagataca ggaaatccca
      841 accctgtggc agtaggtaag gacgatatgt ttcatgcgtt ctgcccgcct tattataaag
      901 atatggggga agcagttgaa gcgcttaatg accttaaatg gacaaactgg gaaactcata
      961 agatgcctta caaagaccct gagggagtgc ttggcgaaat agaaaggccc agcaaagaag
     1021 agattcaggt cgtaaaagat atttgcaatt atatttatga tacttacggc agattccctg
     1081 cattctctga cccaatgttt ttaaggttta tggtgcaggc tcatcatctc gatctggatt
     1141 tttacgataa gtactatccg gagggcgctt atacagaaaa ctgtaaacat catttcggca
     1201 ggtggcatcc agaaatagat gatccgtttg caaacaaata aactgtgacc agccctttaa
     1261 agtggtatgt cagggtcaca gacccagggc ttgcttcatg atgtcaatag gggctttttt
     1321 accagtccag agttcaaagc tctctgcacc ctgatagagg agcattccaa gtcctgtgca
     1381 tgcattctga cctagtgatc tagcatcttt tacaagcctg gtttccttgg ggttataaac
     1441 gaggtcatat accgaggagt ttttgtgtaa tgaatcaagg gcaatgttta attcttcatg
     1501 tcccgtcatt ccggctgaag atgaatttat aagcaggttt gattcactaa ggtgtttgct
     1561 tacagcttgt tcatcaccga gatcaactga actaattacg gtatctgaaa attgttttga
     1621 gaaatattgg gcaaggtctg atgccttact aatcgttcta ttagcaataa caattttttt
     1681 tatccccttc atgcaaagac cagaaagtac tgctcttgct gctccacctg ctcctattaa
     1741 acatgccgaa acatcactaa attctgtaat tcctaattcg atctcaagat cttttaaaaa
     1801 tccgccaaca tcggtgttgt ggccaaaaag cttaccattt ttgttgtgaa cagtgtttac
     1861 agctccagtg tattttgcgg catctgatat atcgtcaagg taattcatta tttcctgctt
     1921 gtgaggaata gttacgttaa ccccctgcac atttaactgt cttatagatt ctacagctgt
     1981 ggaaaggttt tcgggcagta tatcaaaagc aagataaacg cagtttagtc caagatgctc
     2041 aaacgctgca ttgtgcattg atggagaaaa gctgtgactt acagggtggc cgaagattcc
     2101 atagagcttg gttgtggcgt taatatccat aaatgaatca ggttaccaaa ttatcatcaa
     2161 aatattgatt ggctgttttt acatcctgtg aaatagcttg ctttagttct tctaatccgc
     2221 caaatttttt ttcatctctt agcctgctta aaaattctat tctgatttca tcaccataaa
     2281 tttctgagct gaaattaaaa atgtgtgatt ctataagcag gctgtttttt ccaaatgtcg
     2341 ggcggaagcc gatgttggta atactcctat gggtattttt accgaggtga gtgagtgtaa
     2401 tatatacccc tggttttggt agcagttccc agtcggtttt tagatttgct gttggaaaac
     2461 caagtactct gcccctcttt tcaccttccg ttactgtgcc ttctaggctg aagtttctgc
     2521 caagatattt gttggcccta cttatctggc catcgagaat aagatttctt atagctgtgc
     2581 tgcttataac attacttcca tttgtaacag gatcaataat agtggcttta aaccc
//
LOCUS       DBNGSV010000199         3898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_35207, whole genome shotgun sequence.
ACCESSION   DBNGSV010000199 DBNGSV010000000
VERSION     DBNGSV010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_35207"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..>3898)
                     /locus_tag="AC159U_07195"
     CDS             complement(295..>3898)
                     /locus_tag="AC159U_07195"
                     /inference="COORDINATES: protein motif:HMM:NF022100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HIR3336881.1"
                     /translation="YENEIAPVSVPYISTSANKINYERGNITEWYVNKPFGLEQGFTV
                     YSSPNENNDKSNLILQVEYDTRLHPEVKKYGKEIIWKKDNGEVKFNYSKLLAYDSNRK
                     VLPSSMSISDDIISIKIDDNNAVYPIVIDPIMIQETLIHTASDPDVLAKYGQSVGIDG
                     HVAIVGSPGDNDNGDLSGSAYIHTKDSGGVNNWGEFKKITPSDAAACKTFGFSVDVSG
                     DVIAVGANTITTTSSCPTDEGAVYIFSRNQGGVDNWGQVKRLEATDGTVGNQFGFSVT
                     IDGETLIVGAPGDSSGQGAAYVFQKDQGGVDNWGQVAKVAASDGVVDDSFGFASSISV
                     DTAVIGAPAHSIAGVSISGAAYVYDRNQGGADNWGEVSKISASDAADDDVFGISVSID
                     MDTVVVGAGADDLGTTIDAGSVYFFGRDEGGANNWGQIIKLTASDAEDFDALGSAVVV
                     MSDLMFVGSPGDDDGGNGSGAFYGSELSGTWGALEKFTASDAAPGTGALKGLGVFEID
                     DTTGNRSIVSDNTTGTGNLLSEPEGIVVENPGPGEKYLIADKGIPAIISVDPATGNQA
                     IYEDNSGAPSIPFVLPIDLDFDGGGDLFVTDPGSAIVIQIDGVSGSRSIVNNTGSYPA
                     LGFPFGIALEGSGNVVVTDLQMIIENGTQQCFDCALDPDCIPPDCFQFGCVGCVDIIE
                     PSIARVNTSTGNVQQLADTTSCVAGRSFNRPLGIDIMASNNLAVVVIDLNESTSVIED
                     WAILEVNSTTGACSIIADDTTGTGPDLQFPEGIATLASGELAVVDNFRDEVLVIDPAT
                     GNRTVLSGNAIGTGPQFEEPFWVAVNGNGDSIVTDQGTTGISFGSSIDISGRTAIIGA
                     PGKTNLEGGIYIFQFCEQASPIISVSPADQTGTAGETLNYTVSVQNVHNLACASTSFT
                     MSSSVPGGWGSNFNPSTVNLDSGQSTNVDWSVTSDPGAADADYPITAEATDDNEQTFT
                     GSDNANYEIINTIPTPTPTPTPTPTPPPLCNNDGVCSGSEDCNNCPNDCISGSGPSCG
                     NGICETADGEDCNSCPSDCNSKTKGNPKTQYCCGEGGVCGSSQCNNDPFSCTETSSVP
                     YCCGDLICEGAEDTSSCGIDCPPPVCGDAVCESGEDMCTCSADCGTPPVSESMCSDGI
                     DDDCDSDIDCNDIDCSGDLACSCVPTHSKEKGPRCSDGLDNDCDGLIDAADSDC"
BASE COUNT         1070 a          949 c          715 g         1164 t
ORIGIN      
        1 gccggcactt ccaatacaaa catttgtatt agcttgctta tcttactatt tgatagtttt
       61 gtattcttaa gtaacttctt ttgcatagtt tcagcttagt atactttgct gtcctatgcg
      121 agctccatta ttaaattaga tttacgaatt atataaagct taccccaggg gaattttatt
      181 taggaaaata tttgtaaaaa agggaataaa ttaaataaat agagttgagg caactgttat
      241 ttacagttgc ctcaaaatat tataaatccg aaaaattatt acaataaaat ctgtctagca
      301 atcagaatca gctgcatcta taagtccgtc acaatcatta tctaaaccgt ctgaacatct
      361 tggtcctttt tcttttgaat gtgtaggtac acagctgcat gcaagatctc cgctgcaatc
      421 gatatcatta cagtcgatat cgctgtcaca atcatcatca ataccatctg aacacatact
      481 ctctgatacc ggaggtgttc cacagtcagc agaacatgta cacatatcct ctccactctc
      541 acacactgca tcaccacaca caggaggcgg gcagtctata ccacagctcg aggtgtcttc
      601 agctccctca cagataagat caccacagca gtaaggtact gaagaggttt cagtacatga
      661 gaacgggtca ttgttgcact gactgcttcc acatactcca ccttcgccac agcagtattg
      721 agtctttgga ttacccttgg tttttgaatt gcagtcagat ggacatgagt tacagtcttc
      781 accatcagcc gtctcacata ttccgtttcc acatgaagga ccgctgcctg atatgcagtc
      841 atttggacaa ttattacagt cttcggagcc tgaacaaact ccatcgttat tacataaagg
      901 tggaggggta ggagttgggg ttggagtagg agtaggtgtc ggtattgtat taataatttc
      961 ataatttgca ttatctgaac ccgtaaatgt ctgctcatta tcatccgtag cttccgcagt
     1021 aatcggataa tccgcatctg ccgcacctgg atcggatgtc acactccaat ctacatttgt
     1081 tgactgtccg gaatccagat tcactgtgga tggattaaaa ttagaacccc atccgccggg
     1141 tacactcgag ctcattgtaa agcttgtcga tgcacaggca aggttatgaa cattttgtac
     1201 cgagactgta taattgagcg tttccccagc cgtacccgtc tgatctgcag gagagactga
     1261 aataatagga cttgcctgct cacagaactg aaaaatgtat ataccgccct caagatttgt
     1321 cttacccggt gctccgataa ttgcagttct tccggatata tcaatcgatg atccaaagct
     1381 tattcctgtt gttccctgat ctgtaacaat tgagtcaccg tttccattaa ccgcaaccca
     1441 gaacggttcc tcaaattgag gccctgtacc tattgcattt ccagaaagta ctgtccggtt
     1501 gccggttgca ggatcaatca ccaaaacctc atctctaaaa ttatctacaa cggcaagctc
     1561 tccgctagca agtgtcgcta tgccttcagg gaactgaaga tccgggcctg taccggttgt
     1621 atcatctgct ataatggaac atgcacctgt tgtgctgttg acttccaata tcgcccagtc
     1681 ttctattact gaggttgact cattaagatc aatgactact actgcaagat tattgcttgc
     1741 cattatatct attcctagcg gacgattaaa agaccttccc gctacacatg aagtagtatc
     1801 agcaagttgc tgaacattac ccgtggaggt gtttactcga gctattgacg gttcaattat
     1861 atctacacaa cccacgcagc caaactgaaa acagtccggc ggtatacaat cgggatcgag
     1921 agcacaatcg aagcactgtt gagtaccatt ttcaattatc atttgtaagt cagtaaccac
     1981 tacattaccg ctgccctcaa gcgctattcc aaatggaaaa ccgagtgctg gatagctgcc
     2041 agtattatta actattgatc tgctgccact tactccgtct atttgtatta ctattgcact
     2101 tccggggtct gtgacaaata ggtctccacc gccgtcaaaa tcaagatcta taggtagtac
     2161 aaatggaatt gaaggagcac ccgaattgtc ttcatatata gcctggttac ctgtagccgg
     2221 gtctacagaa atgattgcag gtataccctt atctgctatt aaatatttct ctccaggacc
     2281 agggttttca acaactatac cctcgggttc tgataacaaa ttgccggttc ccgttgtatt
     2341 gtcagataca attgatctgt ttcctgttgt gtcatctatt tcaaatactc ccagtccttt
     2401 cagtgcacca gtgccgggag ccgcatccga agcagtaaat ttttcaagcg ctccccatgt
     2461 accgctgagc tcagagccat aaaatgctcc cgaaccattt cctccgtcgt catcccccgg
     2521 agaacctacg aacataaggt cagacatcac tacaactgct gagccaagtg catcaaaatc
     2581 ttcggcatca gacgcagtca atttaattat ctgcccccaa ttattagctc cgccctcgtc
     2641 ccttccgaaa aagtacactg agccagcatc tattgttgtt ccaagatcat ctgctcctgc
     2701 accaacaacg actgtatcca tatcgattga cacagatata ccaaacacgt catcatccgc
     2761 tgcatcagag gctgaaattt tcgaaacctc accccagtta tcggcaccgc cctgattcct
     2821 gtcatataca taagctgctc cagaaattga taccccggca atactatgtg caggagcacc
     2881 gattactgcc gtatcgacac tgatagaaga cgcaaaccca aaactgtcat ctaccactcc
     2941 gtcagatgcg gcaactttag ctacttgtcc ccaattatct actcctccct ggtctttctg
     3001 aaacacataa gccgcacctt gtccgctgct gtcacccggg gctccaacaa ttagtgtttc
     3061 tccgtctatt gtcacagaaa atccaaactg attccctact gttccatcag tggcttcaag
     3121 tcttttgact tgtccccaat tgtctacccc gccttgattc cgagagaata tatataccgc
     3181 tccctcgtcc gtaggacagg aagaagtggt tgttattgta tttgcaccta ctgcgataac
     3241 atctccgctt acgtccactg aaaatccaaa tgttttgcag gcagctgcat ctgatggtgt
     3301 aatcttttta aattcacccc agttgtttac cccacctgaa tcctttgtat gtatatatgc
     3361 agacccagag aggtcaccgt tatcattatc accgggagac ccaacaattg ccacatggcc
     3421 atctatacct acagactggc catattttgc aagtacatcc ggatcactgg ctgtgtgaat
     3481 cagtgtttcc tgaatcataa ttgggtcaat gacaatagga taaacagcat tattatcatc
     3541 aatttttatt gatataatat catcgctgat gctcatagaa gatggaagaa cctttctatt
     3601 agagtcataa gctaatagtt ttgagtagtt aaattttact tctccgttgt cttttttcca
     3661 tattatttcc ttaccgtatt tttttacttc tggatggagt cttgtgtcat attcaacttg
     3721 taatatgaga tttgatttat cattattttc attaggtgag ctgtatacgg taaaaccctg
     3781 ttcgagacca aacggcttat ttacatacca ttctgtaata tttccccgtt cataatttat
     3841 tttgttagct gatgtagata tatatggcac agatactgga gcaatttcat tttcatag
//
LOCUS       DBNGSV010000200         7234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_173250, whole genome shotgun sequence.
ACCESSION   DBNGSV010000200 DBNGSV010000000
VERSION     DBNGSV010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_173250"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..398)
                     /locus_tag="AC159U_07200"
     CDS             complement(120..398)
                     /locus_tag="AC159U_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR3336882.1"
                     /translation="MDINEIEKFKANISVKEAKHLQTKLSAKVIQKNGFKKLSVISGI
                     DLSVIKEQKKLISGIVSFSYPGMEIIERVSRVVDETFPYIPGLLSFRA"
     gene            477..1427
                     /gene="gshB"
                     /locus_tag="AC159U_07205"
     CDS             477..1427
                     /gene="gshB"
                     /locus_tag="AC159U_07205"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR3336883.1"
                     /translation="MGFIVDPIEDFNINTDTTFTFMLESQARGYDVWYFEIKDIFVKD
                     GRPFCNAKKVKVKRAKDHYKVTSTKTLSFEDFDVLWMRKDPPFNMDYVYTTYYLSLVD
                     PKKTFVINDPKGIRESNEKLYTMYFPDVIPQSCVAKDINRLNEFLDEVGGEMVVKPLD
                     GHGGEGVFFVKKGDKNAAVILENITAFGTQFVLAQKFIKKVSEGDKRIIILNGKPIGS
                     VLRVAKKGGEFRCNFHSGGSAKKSSLTKRDMQICDAISERLVADGLYFVGIDVIGGYL
                     SEVNTTSPTCVQEINTLSKVKLEEQVIDFVEEHLNENNIG"
     gene            1435..1725
                     /gene="gatC"
                     /locus_tag="AC159U_07210"
     CDS             1435..1725
                     /gene="gatC"
                     /locus_tag="AC159U_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3336884.1"
                     /translation="MKITTENVKEVAELARLEFAEDEIENFRSQFENILKFISKLDEL
                     DIDDVKPTAHVLDISTPMREDVVQDWITQDEAMKNAPQPEDGFFGVPKVIED"
     gene            1748..3211
                     /gene="gatA"
                     /locus_tag="AC159U_07215"
     CDS             1748..3211
                     /gene="gatA"
                     /locus_tag="AC159U_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3336885.1"
                     /translation="MELHELTIQEISELIKKKEVSPVELTELILGRIEKYDGEINSYV
                     TVLAEFAIKRAREAEKEIGAGNYIGPLHGVPIGLKDIFVMKDYPTTAGSRILEGYTSL
                     YNAAVTQKLIDAGAVIVGKNNMDEFAMGSSNETSYYGQVKNPWDLQRVPGGSSGGSAA
                     AVAASLCYGSVGTDTGGSIRQPASLCGIVGMKPTYGSVSRFGMIAFASSLDQAGPLTK
                     SVYDTALILSLISGHDKRDSTSVKSEVPDYAANLTSDIKGMKVGIPKEYFIDGIDKEV
                     ASAVNDSIKLITELGANIVEISLPHTEYSTAAYYIIAPSEASSNLARFDGVKYGYRTA
                     DADNLSDMYVKTKTEGFGQEVKRRIMIGTYALSAGYYDAFYNKARRVQTLLREDFQQA
                     FEKVDVILTPTAAEPAFKIGEKTTDPIKMYLSDVFTNPANLAGLPGISLPCGYTESGL
                     PIGLQFIGKPFDEQTVLNAAYAYESNTEWNKRRPKIN"
     gene            3222..3392
                     /locus_tag="AC159U_07220"
     CDS             3222..3392
                     /locus_tag="AC159U_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336886.1"
                     /translation="MTEKVKKCIICAKPSEETICEACKANIQGESLDKKMKVEKKVPI
                     AEEVLKRKKKEK"
     gene            3411..4124
                     /locus_tag="AC159U_07225"
     CDS             3411..4124
                     /locus_tag="AC159U_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3336887.1"
                     /translation="MMSKDEFIYDTDELEALKYAKNYYNWIMGYFKPHLGRSVVEVGA
                     GIGTFSDFIIDNINEPYTLSLYEPAGNNFEQLEQKYNGKKKVNIYNDYFKYSNENQFD
                     TLIINNVLEHIEDDQAIVNDFYKSLLPTGKLLIFVPALDFLYSEFDKDLGHFRRYSKD
                     NLRKLLINNGFKIIEIRYFNILGVLSWYFVFKVLRKKTINIDAVKFYDKYIIPVLRNI
                     ESIVKPPIGQNLLVVAKKE"
     gene            4137..4886
                     /locus_tag="AC159U_07230"
     CDS             4137..4886
                     /locus_tag="AC159U_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3336888.1"
                     /translation="MNNFRLSIVIPVYNEVNTIEEILLKVQSVKLDKEIVIVDDCSTD
                     GTREFLELINKKKLKSKSIKTPLNKISLKIDNISVFFQSENMGKGAALHRGFAESNGD
                     IIIIQDADLEYDPEEYHVLLEPIEKGVADVVFGSRFIGGGAHRVLFFWHSLANKFLTV
                     LSNMFTNLNLTDVWTCYKVFKRDILSDIELRENRFGFEPEFTQKIANKKFRIFEVPIS
                     YYGRTYEEGKKIGWRDGIRALWCVIYYKFLG"
     gene            complement(4888..5718)
                     /gene="rsmI"
                     /locus_tag="AC159U_07235"
     CDS             complement(4888..5718)
                     /gene="rsmI"
                     /locus_tag="AC159U_07235"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3336889.1"
                     /translation="MKGKLYVVSTPIGNLEDITLRASRILGEADIIACEDTRVTRKLL
                     SHLGIKKTLLSYHEHNESEKSEEIVNELINGKSVAVVSDAGTPCISDPGYRVVKHASE
                     NGIDVISIPGPCAATSALSISGLPTSGFTFIGFLPKTATKIKDKLQKVKYYPETLIFY
                     ESPNRVINTLSAIQDILGNRHCSVSRELTKIYEETIRGTLSKVIEKLVEKDKIRGEFT
                     IVVTGQNPDLLEPKIDIELELIKLKKTGKTLKEAVSIMTDRFNLQKNDVYKTALKIWD
                     "
     gene            5949..6272
                     /locus_tag="AC159U_07240"
     CDS             5949..6272
                     /locus_tag="AC159U_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3336890.1"
                     /translation="MNLFVGNLDKETTEDELAQLFSEYGDVKSAKIIRDIYSGDPRGF
                     GFVEFGSKSDAINAINEMNGREFKGQELKVNEAIDKRADNKRGGGRRGGKSGGSRKGG
                     SGRRY"
     gene            6626..7165
                     /locus_tag="AC159U_07245"
     CDS             6626..7165
                     /locus_tag="AC159U_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR3336891.1"
                     /translation="MIIRMLIVFITISINSVAENDISLNTVPMVDLKKYSGTWYEIAK
                     IPNRFQKNCISDTTATYNLSDNGRISVVNKCLDKKGKLLVAEGIAKIVDTNSNSKLKV
                     SFFSIFGWHLFWGDYWIIGLGDDYDYAIIGHPDRKYGWILNRTPQLSKEKNEEIFKII
                     ENSGYNPDKFEFPKQDYND"
BASE COUNT         2383 a         1175 c         1513 g         2163 t
ORIGIN      
        1 agagtgaaat gggcttacag aaaccataga ggactggcta aacacaagag aattaattac
       61 ttcaaatggt acaccttttt aaagcaaatc tgactcaaat tagcacacat tttttgcaat
      121 tatgccctga atgacagaag gcctgggatg tatgggaatg tttcgtctac taccctggat
      181 actcgctcaa ttatttccat cccggggtat gagaagctta caatgccgct tatgagtttt
      241 ttctgctcct taattacact caagtcaatc ccgcttatca cgcttagctt tttaaacccg
      301 tttttttgga ttacctttgc gctaagcttg gtctgcaggt gctttgcttc ttttactgat
      361 atatttgcct taaacttctc aatttcatta atatccataa cgtttatgaa cttacattat
      421 ataatttctt gaacaatatg atgtattaga tagattttat ctccatgaag atgcgtatgg
      481 gttttatagt agacccgata gaggatttta atatcaatac cgatacgact tttacgttta
      541 tgcttgagtc tcaggcccgg gggtacgacg tctggtattt tgagattaag gatattttcg
      601 taaaagacgg caggcccttt tgtaatgcca agaaggttaa agttaagagg gcaaaggatc
      661 attataaggt tacttctaca aagactcttt cgttcgaaga cttcgacgtg ctctggatga
      721 gaaaagaccc gccttttaat atggactatg tttatacgac ctattacctg agccttgttg
      781 atccaaagaa gacatttgta ataaacgatc ccaaggggat aagggaatcg aatgagaagc
      841 tctacactat gtattttccc gatgtgattc cgcagtcatg tgttgcaaag gacattaacc
      901 ggttaaacga atttctcgac gaagtaggag gcgagatggt tgtaaaacct cttgacggcc
      961 atggagggga aggggtgttc tttgtaaaga agggtgataa gaatgcggct gtgatacttg
     1021 aaaacataac agctttcggc actcagtttg ttcttgctca aaagtttata aagaaggtat
     1081 cggaagggga taagaggata ataatcctaa acggaaaacc gatagggtca gtgctcaggg
     1141 tggcaaagaa aggcggagag ttcaggtgca actttcattc aggcggaagc gccaaaaagt
     1201 ctagccttac aaagcgtgac atgcagattt gtgatgcaat atcagagagg cttgtggcag
     1261 acgggctgta ttttgttggc atagacgtta taggcggcta tttgtcagaa gtaaatacaa
     1321 caagcccaac atgcgtacag gaaataaaca cacttagtaa agttaaactg gaagaacagg
     1381 tcatagattt tgttgaagaa catcttaatg aaaataatat aggataaaat tgttatgaaa
     1441 attacaacag aaaacgtaaa ggaagtggca gagctggcaa ggcttgagtt tgctgaggat
     1501 gagattgaaa acttcaggag tcagtttgaa aacattctca agttcatatc gaagctcgat
     1561 gaactggata tagatgacgt aaaacccaca gcgcatgtgc ttgatatttc cacaccaatg
     1621 agagaggacg tagtacagga ctggataaca caggatgagg caatgaaaaa tgcccctcag
     1681 cccgaggatg gtttcttcgg tgtccccaag gtaattgaag attagttaag gtattagagg
     1741 attagttatg gagctacatg aactaacaat acaagaaata tcagaactta ttaagaaaaa
     1801 agaagtctct cccgttgagc ttactgaact tatactcggc agaatagaga agtatgatgg
     1861 tgagataaat tcatatgtca cggtacttgc tgagtttgca attaaaagag ccagggaagc
     1921 agagaaggaa ataggtgctg gcaattatat agggccactg cacggagtgc ctatcggcct
     1981 gaaggatata tttgtgatga aagattatcc taccacggca ggatcgagga ttcttgaggg
     2041 gtatacctct ttatataacg cagctgtcac acaaaaactt attgatgcag gagccgtaat
     2101 agttggcaaa aacaatatgg atgagtttgc aatgggttcg tccaatgaga catcgtatta
     2161 cggacaggtt aaaaacccat gggatttgca acgtgtacca ggggggtcaa gcggcggatc
     2221 ggcagcggca gtggcagcgt cactctgcta tggctcagta ggtaccgaca ccgggggctc
     2281 aattcgccag cctgcatcac tatgcggtat agttggtatg aaaccaacat atggatcagt
     2341 gagccgattt ggcatgatag catttgcgtc atctctcgat caggcaggac cccttacaaa
     2401 atctgtatat gacacggctt tgatattgag cctgatatcg ggccatgata agcgtgattc
     2461 tacttcggta aaaagtgaag ttccggatta cgctgctaat ttaacttccg atattaaagg
     2521 catgaaagta ggaataccaa aggaatattt tattgatggt atagataaag aggtagctag
     2581 tgcggttaat gattcaatta aacttataac tgagcttggt gcaaatattg tagaaatatc
     2641 actgccccat accgaatact ctactgcagc ttactatata attgccccgt ctgaagcaag
     2701 ctccaattta gcaagatttg acggcgttaa atacggatat agaacagcgg atgccgataa
     2761 cctttccgat atgtatgtaa aaacaaaaac cgaggggttt ggtcaggaag taaagagaag
     2821 gataatgatt ggaacatatg cgctatcagc agggtactat gatgcatttt ataataaagc
     2881 cagaagggtg cagactttgc ttagagagga cttccagcag gcgtttgaaa aagtggatgt
     2941 aattcttacc ccaactgctg ccgagcctgc ttttaaaatt ggagaaaaaa ctactgaccc
     3001 cattaagatg tatctgtctg acgtgtttac aaaccctgcc aacctggcag gccttccagg
     3061 aatctcgctt ccctgcgggt atactgaaag cggacttcca attgggctgc aattcatagg
     3121 caaacctttt gatgaacaga ctgtcctaaa tgctgcttat gcttatgagt ctaataccga
     3181 gtggaataag aggagaccta aaataaattg aggtagatga tatgactgaa aaagtaaaaa
     3241 aatgcataat ttgcgccaaa ccttctgaag aaactatatg tgaagcctgt aaggcaaaca
     3301 tacagggtga atcgcttgat aagaaaatga aagtagaaaa gaaagtgccg atagcagagg
     3361 aagtactaaa gaggaagaaa aaagaaaaat agtatttttc tttcttggaa atgatgagta
     3421 aagatgagtt tatatacgat actgacgagc tggaagcact aaaatatgca aagaattatt
     3481 acaactggat catgggttat tttaaacccc atctgggccg ctcagtagtg gaggtaggag
     3541 ctggaatcgg gacattctct gattttataa tagataacat taatgagcca tataccttgt
     3601 ctctttatga acctgccgga aataattttg aacaacttga acaaaagtat aacggtaaaa
     3661 aaaaagtaaa catatataac gattacttta agtatagtaa tgaaaatcaa ttcgataccc
     3721 tgataatcaa caatgttttg gaacatattg aagatgatca ggcaattgtt aacgactttt
     3781 ataaatctct attgcccact ggtaaacttt taatattcgt tccggcgctg gattttttgt
     3841 atagtgaatt tgataaagac ctgggtcatt ttagaagata ttctaaagat aatttgcgta
     3901 aattgctcat taataatggt ttcaagatca ttgagatcag atattttaat atacttggtg
     3961 ttttgagctg gtattttgtg tttaaggtat taagaaagaa aacaataaat atagatgcgg
     4021 ttaagtttta tgataaatat ataattccag ttttacgaaa cattgaatca atagttaaac
     4081 ctccaatagg gcagaattta ttagtggtcg ctaaaaaaga gtaatatttt aaaaagatga
     4141 ataatttcag attatctatt gttattcctg tttataatga ggttaatacc attgaagaaa
     4201 tacttctaaa agtacagtct gtaaaactcg ataaagagat agtgattgta gatgactgct
     4261 ctacggacgg tacaagagag tttcttgaac taataaataa aaagaaactt aagtctaaat
     4321 ctataaaaac cccgctgaat aagattagct tgaaaataga taacatcagt gttttctttc
     4381 aaagtgaaaa tatgggcaag ggtgctgctc ttcatagagg ctttgctgaa tcaaatggag
     4441 atatcattat tattcaggat gcagatcttg aatacgaccc agaggaatat catgtactgc
     4501 tcgaacccat agaaaaaggt gtagcagatg tggtatttgg ctcaaggttc attggtggag
     4561 gtgcacacag agtgttgttc ttctggcatt ctctggcaaa caaatttctt actgtccttt
     4621 ccaatatgtt taccaaccta aacctgactg atgtttggac gtgttataag gtctttaaaa
     4681 gagatatcct gagtgacatt gaactgagag aaaatagatt tggatttgaa cctgagttta
     4741 ctcaaaaaat tgcaaataaa aaattcagga tttttgaggt gccaatttct tactatggaa
     4801 ggacctatga agaaggtaaa aagataggtt ggagagacgg gatcagagcc ctttggtgcg
     4861 tgatttatta taagttttta ggatagatta gtcccagatc ttaagagcag ttttgtaaac
     4921 atcgttcttt tgtaaattaa atctgtcggt cataatactt actgcttcct taagtgtctt
     4981 ccctgttttt tttagcttga ttaactctaa ttcaatatct attttcggtt ctaataaatc
     5041 cgggttttgg cctgtaacta caattgtaaa ctcaccccta attttatctt tctctacaag
     5101 cttttctatt actttagata aagttcccct aatagtctct tcatatattt tcgtaagctc
     5161 tctgcttacc gagcagtgcc tgttgccaag aatgtcctgt atagcgctta aagtattaat
     5221 aaccctattt ggcgactcat aaaatataag agtctcagga taatatttta ccttttgaag
     5281 cttatctttt atttttgtgg cagtttttgg taaaaatcca ataaatgtaa agccggatgt
     5341 tggaagcccc gaaatactga gtgccgatgt agcagcgcag ggccctggta ttgagatcac
     5401 atcaatacca ttttctgatg catgctttac aaccctgtat cccggatcag atatgcatgg
     5461 tgttcctgcg tcagaaacaa ctgctacgga ttttccattt attagctcgt ttacgatctc
     5521 ctctgatttt tcgctttcat tatgctcatg gtaacttaac agggttttct ttattcccag
     5581 gtggctaagc agttttcttg taactcttgt atcttcgcat gcaattatat cagcttcacc
     5641 aaggattctt gatgccctga gagtaatatc ctcgagatta ccaatagggg ttgagacaac
     5701 atataatttc cctttcatgt ctttattata gtgggatatt atcagattga taatatatta
     5761 tcattatcat gtattattag gatgtaaact caatcaattt gctaattcat gtagtaagtg
     5821 taatatccgt agttaacaca ttcgatctct attaaatttt tcttttcttt taggttttta
     5881 taaactgcct tccttaaatt ttataaaaat ctgcaaatct ttaatacgaa atcacaaagg
     5941 ggacaaatat gaatcttttc gtaggaaatc tggataagga aacaacagaa gatgaacttg
     6001 cgcagctttt tagtgaatat ggcgacgtaa aatctgctaa aattataaga gatatatata
     6061 gcggtgaccc aagagggttt gggtttgtag aattcggaag taaatccgat gcgattaatg
     6121 ccataaacga gatgaacggc agggaattca aaggacagga actaaaggtt aacgaagcca
     6181 tagacaagag agcagataac aaacgaggcg gcggcaggag aggcggtaaa agcggcggca
     6241 gcagaaaagg cggcagtggg agaaggtatt agatttactg aattatacca aaaaaagata
     6301 acactatatt aattaagtta cagagccgac gctggaattt gaaccgcaga tccgcttatt
     6361 acaattgaag attatatagg ttgtgttttt tttagtcaaa atgtttttaa ctgagaaaat
     6421 tctttattat cagggtgtta taagccgatt aattaaaaac ataaggtatt taaaaaatct
     6481 taagtgtata aaccaagagc aaaactgcag taataaaatt gtattgtttt agtgtttggg
     6541 attaaagctt ttagccatgc gtaactaaga atatctattc cacacgactt tcattatgtt
     6601 gctgaagaaa gggtataata ttaatatgat cataagaatg ttgattgtat ttataaccat
     6661 atcaataaat tctgttgctg aaaacgatat ctctttaaat acagtaccaa tggttgacct
     6721 taaaaaatac agcggcactt ggtacgaaat tgctaaaatc cctaatcgct ttcagaaaaa
     6781 ttgtatttcc gatactactg caacttataa tttatctgat aatggcagga ttagtgtagt
     6841 aaataaatgc cttgataaaa aaggcaagct tttagtcgcc gaaggtattg ccaaaatagt
     6901 agatacgaat tcaaactcca aactcaaagt aagctttttt agtatattcg gatggcactt
     6961 attctggggt gattattgga ttataggact tggtgatgat tatgattatg caatcatagg
     7021 acatccagat agaaaatatg gctggatatt aaatcgcaca cctcagctat ctaaagaaaa
     7081 aaatgaagag attttcaaaa ttatagagaa cagtggatac aatccagaca aatttgaatt
     7141 tccaaaacaa gattataatg attaagtaga tttaattact tatttatata ggatattagg
     7201 agattactga ttaataggta tagaggttca aaga
//
LOCUS       DBNGSV010000201        17828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1697817, whole genome shotgun sequence.
ACCESSION   DBNGSV010000201 DBNGSV010000000
VERSION     DBNGSV010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17828
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1697817"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="AC159U_07250"
     CDS             complement(<1..1462)
                     /locus_tag="AC159U_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336892.1"
                     /translation="MSESRIENKLLNDHADCLERVYIEGQVPCVVFYKGDSPDEYTLK
                     LYNIFSNRGFKSYLVPEDFLIKDKQDLKVYEEIINSACLFIFYIDENRSSFLYCLGHI
                     RAKSRPILTITREKLLEDISSDYSSEFKYDSGFYEIDYGKLSEIGQESNSLVNFIASV
                     DHSKNFVLSSKDSDNEISDIYASFDQIIEYIKPDLQSLFTEDLNDKLSGDLEPEYKDI
                     IDTYFGLNNTDSDKLIKYLDIKPKDKSLKNSSAYLLFLNIVLTNIINYLSNKYSDKNY
                     KLVLDKYEEIIKNTEITDEKKHTVHSIIGLENKGAACLILSCLKHDDSFLEESEKLFE
                     KSSKLKKTNLLKDIFSGAKINYALLQEYLADSTGSVDSFNNTLELLKDADIANDGFDP
                     AFKDGLALNNKAICLRKLSPVTKNQSELQLAAEQSEKAFKIFESSNRTLHKISALFNI
                     SASLLYESKLANEKKFCTEALEKLDQAFDLLDKNTDV"
     gene            1692..2792
                     /locus_tag="AC159U_07255"
     CDS             1692..2792
                     /locus_tag="AC159U_07255"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3336893.1"
                     /translation="MDNHKSKILIVDDEPRNRLLLNNMCENMGYKTFQATNGEEAIDY
                     AVDNSPDLIIMDVIMPKMNGFDSTEKIKADDRTKQIPVIIVTALGSREDLLTGISKGA
                     DDFLTKPYDFEELAMRIKNNLKIKKYNDLLKENNDILEEKVQQRTQELQKALFELNLA
                     HDSTKAAYLETIYRLTLATEFKDQATGSHIKRISLYTRKIAETLSMNTEFIENIFHAS
                     PMHDIGKVGIPDSILLKPGALNAEEWEIMKTHTTIGAKILEGSESTFLKMGQEIARNH
                     HETWDGSGYPNGLSKTQIPISARITIIADQYDALRSTRPYKKPFTHDEALKIITEGDG
                     RTSPDHFDPDIIKAFIEINKDFGEIFESYSGY"
     gene            3220..3498
                     /locus_tag="AC159U_07260"
     CDS             3220..3498
                     /locus_tag="AC159U_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336894.1"
                     /translation="MKDERGLDMKNTILGLMLGTMFLSWGGIDEARAGLTPPQQNLFN
                     AIIHSCEKKFFACYTDKALTASIINSALSVPPETVPGLCDAVCNSSAI"
     gene            complement(3969..4493)
                     /locus_tag="AC159U_07265"
     CDS             complement(3969..4493)
                     /locus_tag="AC159U_07265"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR3336895.1"
                     /translation="MKIAILSILSFCVLGIQGVIAEIPIMAKSKLKNSAGEVVGVATF
                     LESNDGVNIAVQVRSLTPGLHGIHIHETGKCKAPDFKSAGGHFNPFAVSHGLRNPKGY
                     HVGDLGNILIKEDGTGTMLTLSSLATLQGSKNSLFRKGGTALVIHSGPDDLISDPTGN
                     AGSRVACGVIKMIK"
     gene            complement(4594..4962)
                     /locus_tag="AC159U_07270"
     CDS             complement(4594..4962)
                     /locus_tag="AC159U_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336896.1"
                     /translation="MDNQEDILDKFRAGLNRGLRIVNVRSKEAYDVLKTKNNIQGKNR
                     NKRKLVEELGNAVFRTYKHKGNISEDSIKNKCEDILKMESEIDDLNEQIQNIHENALK
                     ELGKLKAITKPNDDSDDGKE"
     gene            5160..6401
                     /locus_tag="AC159U_07275"
     CDS             5160..6401
                     /locus_tag="AC159U_07275"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3336897.1"
                     /translation="MANNNLFYGWYIVAASLILIVLDGLLLYSFGIFLPGLNDKFGLS
                     EAGGSSIYALRSFVLAFSLAISGRLVDKYDPRIIIFSGGLICAIGLLLCGFAETKFEL
                     FLYFGVLIGIGDGVLYITCVTLISRWFVKKRSLAIGIITTGVPLSGLFVPPITTWLII
                     NHGLSNAFFGLAAFVFIAIFAALFLRKSPADMNMKPYGAEDIGKDGNNTLKSPEESKD
                     WEAKEAIKTPAFWLLYVMWLFGFITFLIIITHLFNYEIHVGIPELLASGAPAAIGLGS
                     IFGRIVLSGFLTERMRSERILFICFFVQSSSIIILLLFKDIEYYFIFGLLFGFFYSGW
                     VPIFPMILGDFFGLKALGAIYGFFGTSFSIAAIIGPILAGYLFDRTGSYFYPFLIAII
                     ICYLAALLTFFIKKPERKITA"
     gene            6421..7896
                     /locus_tag="AC159U_07280"
     CDS             6421..7896
                     /locus_tag="AC159U_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336898.1"
                     /translation="MEFKVDKDFTESAEKLLGRLNYHKYFFDSGISLQSNCKDILDDH
                     RELFSPDVLADLDRSIYNENDHEKIELFKVFVSNMIQTYYGSEHIDRLNNEFIKQAQN
                     PENKGKNLLNLIPAIKTERKREKRNKLGKQYSELSKELYHNLELVIEKRNETSTLLGC
                     NDYIDQTRSLCSLDVESTEKLCNDFLSDTEYIYKDLLGWQLKNKLEIRIDDARHEDIL
                     FLFNSYELKDYFKQTSADKLSTRFLDEMGINLPGSIKFDLNNRLTKNPDARTYPVSVP
                     DNILISVNRIKTAEDYDSILGQIGKSLLLGSINIDDSFEKKRLIDPTTLEIFKFLFED
                     LIFEQNWLQRYLRLDTDKNFLEFLYLKKLFQIRNLCLNVLSYRLIYENNLHQALEQIS
                     ELYQKSSFIKPNEHMVLLNLILDKDTPYYSFRACLLESYLREYLISRFDEQWWREIDA
                     GRQLIKWWNQNSGLTTGFLEQEIETGPLNIKKLIKYFEKAF"
     gene            8071..10113
                     /locus_tag="AC159U_07285"
     CDS             8071..10113
                     /locus_tag="AC159U_07285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3336899.1"
                     /translation="MKILTAGYLVFINSSISLALIILVYKKQNNIVGLFANDVNKISQ
                     FLKSLNSPYIIFLLILLCLVSFWLFLSALLLPPRGIDDLTYHLPAVYEFINHEKIFLV
                     AINYRGHFAFPMNAELLFLWPVIFIKNLIPIGLVQYFYGLYAVVVIYALSKEMRLKTQ
                     TSMFIGLCFFFSPVVLAQAGVGYIDLIVSVFYLTAIFFSVRFYRTQRPIYLYLCAVSI
                     GLIVGMKYTMLLCALVLQVLILPGLSRKKLKQAGLYLFIITIFCSYWYLRNFLILGHP
                     FYPFNISGLESTYTSFSDLSVLNIISKYLTILKLFFTKDWGIGSYNGGYGIIFWGLAF
                     PSWIYLFVKSIISKSENRLINLFMWIQIPVGFTFFLIVPIDEFHLYPRFSIFMISIGL
                     IAFGMVIEKFNKNQIYKNAVLFCICIFCFVSTFQLARAKLPSHAIDAPIYDYINKKNY
                     SNIRYLRVASTSASFLWETLDFITLNNETGLDCYFASSLLDYVGPIYGSKLQNRVWNF
                     KEDKSSSPDALIFLIHEEDKVKYYGKKITIDEVINNIDYVLIDIAQKAYLYLNKDFLD
                     KDSEIQKSLIKHYENYYNKEIKVASTLRNKINTDTPIIASDYYGIGLQYLSMTENIRN
                     SVLMLPEGKENKFLGENVTGSFYSVNKKYKGYNSKLVYKIRINKDENVILYLNSKK"
     gene            complement(10324..11322)
                     /locus_tag="AC159U_07290"
     CDS             complement(10324..11322)
                     /locus_tag="AC159U_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3336900.1"
                     /translation="MKAAIYHEFGEADVIKLEEYDKPQISDDEVLINVKSVSLNALDL
                     RLRSGKSPRPVDLPHIGGIDIAGEIAEVGGNAGDLNVGDRVVVDPTVRLEKGFEVIGV
                     NRHGGLAEFVKVPAVNVITIPEGLSFDDAAAAPICTVTAWYGLVERAGLKEGERVLVH
                     AAGSGTGCMAVQIAKHLGASQVIATAGSDEKLQKAKELGADEIINYNTQDFAEEVKNM
                     TGGKGVNVIFDQIGASVYEKNIQSLSAKGRLLLVGVVGGVKAELMFGPLIMKDLEVLG
                     VTVFNAPRSNLEKALKLTLNGYIKSVIDKKFPLSETAAAHKHIENRAQFGKVIVNP"
     gene            11408..11641
                     /gene="tatB"
                     /locus_tag="AC159U_07295"
     CDS             11408..11641
                     /gene="tatB"
                     /locus_tag="AC159U_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR3336901.1"
                     /translation="MFGIGTGEILVILVVALLVLGPNEIPKVARTIGKTMKDINRFKN
                     DLRQSVDTEFEQYEQSEQKKKEEKPDKEKDKIT"
     gene            11677..12216
                     /locus_tag="AC159U_07300"
     CDS             11677..12216
                     /locus_tag="AC159U_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HIR3336902.1"
                     /translation="MSKLTEPAPVKIIFSVIFARGADTDFLKQILIENFGQLDFESQI
                     INFDKTGYYTEEMGAELFRKIYSKKEVVKREQIIDIKIKANDIEEQTSVNNKRVFNID
                     PGYLATEHFVLSTAKGYAHRPYLGKGVYADLTLIYRDKNFSELEWTYPDYKSSELQNI
                     LKEIRVNYIKNLREEKMYD"
     gene            12209..13075
                     /locus_tag="AC159U_07305"
     CDS             12209..13075
                     /locus_tag="AC159U_07305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR3336903.1"
                     /translation="MISMTGFGRTELQTSLGTVAIEARSENHRYLDVTTQLPDILSEI
                     ENDIQEIVKKHISRGKFRIYISAKLNNKSSHLDYSLAKDSLNNLEKLKKDLKLNGDRK
                     LEHLLMLKDLFTTESKEELTKADINKVLNSLKSVIKKVHDARKAEGKYLKSELLKRIN
                     KIEKLLAKIKTRRKNYPKETKEKLKERVNLLLEDSQIENSRLVQEIAILAEKSDFTEE
                     TVRLNAHIKRFKDTMNKNTPVGRELDFLIQEMNREAGTISAKSKDAKISHFIIELRSD
                     LEKIREQIQNVE"
     gene            complement(13083..14015)
                     /locus_tag="AC159U_07310"
     CDS             complement(13083..14015)
                     /locus_tag="AC159U_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336904.1"
                     /translation="MYPKYDPSGRIKFDYNEQFNIRIGKKNYSGSQIKNTGDYDTYVE
                     FNKYGFRDKKDIAEIKPDELAVVGDSFSLGWGVDEHKRYSDQLQKLINKPVYNISISN
                     NLNGYRELLNYAESTGAKIKNVILGLCMENDLLDYGNRNSNRIVNPSKYNKIKHFLYN
                     FALFNLFATYVHSNSELRDLAVRYGLIIDNLDGIPKNEIDKNVLNSSVHILSDLDKKY
                     NLLVVIIPSRGLWVGKNKQIESEVHNKLISMLSDKEIEFIDLRSEFEKGRNPLGYHFE
                     NDGHWNVHGHSLAAKQIYDYLIDCEHSTCIPCLN"
     gene            14226..14639
                     /gene="ndk"
                     /locus_tag="AC159U_07315"
     CDS             14226..14639
                     /gene="ndk"
                     /locus_tag="AC159U_07315"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR3336905.1"
                     /translation="MEKTLSIVKPDGVEKNVIGEVIRRFESEGMKVIGLKMVKLSKEQ
                     AQGFYAVHKERPFFDSLTDFMSSGPCVLMVLQGENAIKTVRTTMGATNPAEADNGTIR
                     KDFASDIEHNIVHGSDSTESADYEIVYFFNDLELA"
     gene            14661..15311
                     /locus_tag="AC159U_07320"
     CDS             14661..15311
                     /locus_tag="AC159U_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3336906.1"
                     /translation="MANRKNKRAYSTALFIFLAVLCLYLFRNSYLPAAGNYLVIQDDL
                     EKADAIFVFGGSVPNRIIEAAEIYKKGYAPLIIISKYPRPGGYEYLKNKGISYPEGHD
                     INKSIAQKLGIPKKSIVITKDRNTSTFEELVRLNKFCKNNNFNKVILVSTKSHTKRIS
                     KIFTDISEGKIKGIVRYTNYDSYDPKNWWKDRSSLRQTLFEYQKLLHHFFVDRKKI"
     gene            complement(15308..15961)
                     /gene="lipB"
                     /locus_tag="AC159U_07325"
     CDS             complement(15308..15961)
                     /gene="lipB"
                     /locus_tag="AC159U_07325"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3336907.1"
                     /translation="MNKNVSWSLLGRISYSDCLDIQNRLQNDIVSGKDCSYGHILMLE
                     HEPVITVGKFGSNQNLLATQKVLKERGIELFETNRGGDFTYHGPGQLVCYPVLNLKEL
                     GLGVKKYVNLLEEVIIQSLRSLEIGACRRDKYPGVWVGNSKIAFIGIYVKKHITMHGF
                     SLNITKQDDAFSHIVPCGITDLQITSISEILKREVELKEIINIVKDNFENIFEIEFD"
     gene            16007..17002
                     /gene="holA"
                     /locus_tag="AC159U_07330"
     CDS             16007..17002
                     /gene="holA"
                     /locus_tag="AC159U_07330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3336908.1"
                     /translation="MAKTTKNSLTYSELKDRLQKELLDPVFILHGDDEYLIEQSINEI
                     KQSVEQKNPDADYLSYYADSVNITEILDSANSMGLFSAAKIIVIKQCEKLLKDDAGYL
                     MEYIDNPNKQTFMVLVFENLKKFKIKPNDNFISVNIPSDSKNIILKVKKESEKQGYHI
                     KNDAILRLTELVGENLIDILGEIKKLSINQSGDIIEKSDVENYIKKSKFKDVFELINA
                     ICNKDKSKAYEILSELEQNGEEPIAVINRISWRIRQLWQVNQLVNEKKSKDEILKTLK
                     ISSGNLYYMQKQMKNFRNEEYIRILDTIYNTELKLKSTATPRFDLLTSSVMQMCR"
     gene            complement(17025..17306)
                     /gene="rpsT"
                     /locus_tag="AC159U_07335"
     CDS             complement(17025..17306)
                     /gene="rpsT"
                     /locus_tag="AC159U_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3336909.1"
                     /translation="MALRSKSGIKRDRQSKVRRERNLHIKSTLRSKVKTLLSTIESKD
                     LEKSKELLKDTTVAFDKAATKGVIHKRTASRNISNFSKKVHKLEAAAQA"
     gene            17445..17720
                     /locus_tag="AC159U_07340"
     CDS             17445..17720
                     /locus_tag="AC159U_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR3336910.1"
                     /translation="MNINFNSTIKFPSEVIIQQTADGQSIMLNTRTENYFGLDETGTI
                     FYNNLINSLNINKALQKIHNTYEVEKDTLSTDLQSFISDLKKNKELA"
BASE COUNT         6013 a         3142 c         3003 g         5670 t
ORIGIN      
        1 ttacatccgt atttttatcc aataagtcaa atgcctgatc taatttttca agggcctcag
       61 tacaaaattt cttttcattt gcaagctttg attcatataa aagcgaggcc gagatattaa
      121 atagggcaga tattttgtga agcgttctat tacttgactc aaatatttta aaagcctttt
      181 cactctgctc tgcggccagt tgcagctcag actgattctt ggttacgggg gagagttttc
      241 taaggcatat ggctttatta ttcaaagcca gaccgtcttt aaaggctgga tcaaacccat
      301 catttgcaat atctgcatcc tttaagagct ccaatgtgtt attgaagcta tctaccgagc
      361 cggttgaatc tgcaagatat tcttgcaaaa gagcatagtt gatcttagct ccagaaaaaa
      421 tatcttttag aagattagtc ttttttagct tagaggactt ttcgaatagc ttttccgatt
      481 cttctaagaa actgtcatca tgttttaaac aactgaggat taagcatgcc gcacccttgt
      541 tttcaaggcc tataatgcta tgcactgtgt gttttttctc atcagtaatt tctgtatttt
      601 ttataatctc ttcatactta tccaatacaa gcttatagtt tttatcggag tatttattgc
      661 ttagatagtt aattatgtta gtaagaacaa tgtttaaaaa taataaatag gcagaactat
      721 tcttaagtga tttatccttg ggctttatat ccagatattt tattaattta tccgaatcag
      781 tattgtttaa tccaaagtat gtatcaataa tatctttata ctccggttcc aagtcaccag
      841 acagtttatc gttcagatct tcagtgaata acgactgtag gtcaggctta atatattcaa
      901 tgatttgatc aaagcttgca tatatatcac ttatttcgtt atcactatcc ttactgctca
      961 atacaaagtt tttagaatga tctaccgatg ctatgaaatt aacaagagaa ttgctttctt
     1021 gcccaatttc gcttagcttt ccgtagtcga tttcgtaaaa tcctgaatcg tatttaaatt
     1081 cactgctata atcagaactt atgtcttcta agagcttttc acgagtaata gtgaggatag
     1141 gacggctctt tgcccttata tgaccaagac aatacagaaa acttgatctg ttttcatcaa
     1201 tgtaaaatat aaacaggcat gcgctgttaa taatctcttc atatactttt aaatcctgct
     1261 tgtctttaat aagaaaatct tctggtacaa gatatgactt aaacccccgg tttgaaaaga
     1321 tattatataa cttaagagta tattcatcgg gactatcacc cttataaaaa actacacacg
     1381 gtacctgacc ttcaatgtag accctttcca agcagtcggc gtgatcgttt aacagcttat
     1441 tttctatcct ggattcactc atttaatata atttattata attttatatt actacgcata
     1501 ttaatacttt agtaatactt atactaaccc cgtaagaatg taaatattaa ttataaacct
     1561 gatatcgggt tattaaaaca gttatataaa aaataactga tattataaaa cattataaaa
     1621 attattgtac aattgaaata aaatttataa attataaaat gttatatata tataacctaa
     1681 agaatattct tatggacaat cataaatcaa agatactcat agtagacgat gaacccagaa
     1741 acagactgct tctcaataat atgtgcgaaa acatgggata caaaacgttt caggcaacaa
     1801 acggcgaaga ggcaatagac tatgcggtag ataacagtcc cgaccttata attatggatg
     1861 ttatcatgcc aaaaatgaat ggatttgatt caacagaaaa aatcaaagcc gatgatagaa
     1921 ccaaacagat tccggtaatc atagtaacag ccctaggctc gagagaggac cttcttacgg
     1981 gtatttccaa aggggccgat gacttcctca caaagcccta tgattttgaa gagcttgcaa
     2041 tgaggataaa aaataacctc aagatcaaaa aatacaatga ccttctaaaa gagaataatg
     2101 acatattgga agaaaaggtt caacaaagaa cgcaagaact gcaaaaagcc ctttttgaac
     2161 taaacctagc acatgatagc accaaggctg cctatcttga gactatttac agactcactc
     2221 ttgctactga gtttaaagat caggccacag gctctcacat aaaaagaata agtttatata
     2281 ccaggaagat agctgaaacg ctttcaatga acactgaatt tattgaaaat atattccatg
     2341 cttctcctat gcacgatata ggaaaggttg gaatacctga cagtattctt ttaaaacccg
     2401 gtgcattaaa tgccgaggaa tgggaaataa tgaaaactca tactacaatt ggggcaaaga
     2461 ttttagaagg atcagaatct acattcctga aaatgggtca ggaaattgcc agaaaccatc
     2521 atgaaacatg ggatggctca gggtatccaa acggccttag caaaacacag ataccaattt
     2581 cagccaggat aacgattata gcagatcagt acgatgcact aagaagtaca aggccgtata
     2641 aaaagccatt tacccatgat gaggctttga agataattac cgagggtgat ggaagaacaa
     2701 gccccgatca ctttgacccg gacatcatta aggcttttat agaaataaac aaggattttg
     2761 gagaaatatt cgaatcctac agcggatact aattcgtatt tctcttcata tttctattac
     2821 agaagtctac tgtgctcata gagcctaata ttaatcaaaa taatagttat taatatatac
     2881 aaatttggtt acttaattac tgagcattta caagtgcccc cttttattca gtcccctttt
     2941 tatgtcacta attaggaagg ttttcagcag aggattaata aaaaaataca cttagaatat
     3001 ttattaacat aagttatctt atcggattta gagtaataaa cgccagatat gtaaaatcaa
     3061 ttgtgtgata attgaatata tgtaaaacat catgtccccc attattttgt ccttttataa
     3121 taagtcattg actttacata ataaaactaa tagagcttga agagagaatg aaacttaagt
     3181 cctgttttgt catcttatgt agtggtagga cttaaatcta tgaaggacga aagaggcttg
     3241 gatatgaaaa acacaatatt aggacttatg ctgggaacaa tgtttcttag ctgggggggg
     3301 atagacgaag ctagggcagg actaactcct ccacagcaga atttatttaa tgcaataatt
     3361 cacagctgtg agaagaagtt ctttgcatgt tatacagata aggctctcac agcttcaata
     3421 ataaatagtg cgctgagtgt gccgcccgaa actgtacctg gactttgtga tgctgtttgt
     3481 aattcatcag caatctaaga aagaagaact agcaattgtg ccgatgtaca gtgctcacag
     3541 atatgtcagg ttcttggtgg tatacaggac aatgatattg agcaacttat agaacaaaag
     3601 aattttgatg agttagggga agtttgtcag cttggtgtgc ctgaccagag ttaacatacc
     3661 gtagtgttgc aaaaaggaac gtttttgcaa tagttaattg atcccgtata agttcctgaa
     3721 attattatat tcttactgtt gcataattct gttgcaaaag gaatatctag ctctgaggcc
     3781 caacaggatg taaaaatcac tttctcagga tagcaccaat attgtataag ttccttaaat
     3841 gtgcggacac ttcactcttt ttgataacaa agacatttaa gtaaaagggg actccactct
     3901 tttttggtta gaagatgtat gctttagagt atgccaagac agccaaagta aatattgtct
     3961 acaactattt acttaatcat tttaattact ccacaagcaa ctctgctgcc tgcattgccg
     4021 gtaggatcag aaataaggtc atctgggccg gagtgtatta ccaatgccgt accgcctttt
     4081 ctaaatagag aatttttgct gccttgcagt gttgcaaggc ttgaaagtgt aagcattgtg
     4141 cctgtgccgt cttctttaat aaggatattt cccaaatctc cgacatgata gcccttgggg
     4201 tttctaaggc cgtgacttac agcaaatgga ttaaaatgtc caccggctga tttaaaatcc
     4261 ggagctttgc actttccagt ctcatgaata tggataccat gaagtcccgg ggttaagctc
     4321 cttacctgca ctgctatgtt taccccatca ttgctctcta aaaaagtggc aacacctaca
     4381 acttcaccag cgctgttttt taacttagac tttgccatga taggaatttc tgctattacg
     4441 ccctgaattc caagaacaca aaatgataaa atagataaga ttgcaatttt cataattatt
     4501 tctcccttta ttactaatat ttatttaact tattttcaaa ctctgattca tgtccaagca
     4561 taattatctg ccaactaata ataaggtatg aaatcactct ttaccatcat cgctatcatc
     4621 atttggcttt gtaatagcct ttaatttacc aagctctttt aatgcatttt catgtatatt
     4681 ttgtatctgc tcatttaaat catcaatttc tgattccatt tttaatatat cttcacattt
     4741 attttttata ctatcttcac taatattgcc tttatgttta taggtcctga atacagcatt
     4801 tccaagctct tctactagtt ttcttttatt cctgttttta ccctggatat tatttttggt
     4861 tttgagtaca tcatatgcct cttttgagcg tacatttacg attctaagcc ctctgttaag
     4921 gcctgccctg aatttatcta atatatcttc ctggttgtcc atgtcatatt cctttttata
     4981 tatgcgtgct gattttaata tattatcatt gttaaggatt attgattaaa aatcaaaaaa
     5041 aaactgctta tttaatttat ttatttatat aatagataaa taaatcttgc ctcttaagac
     5101 atactcactt aaaatctgag ggcttattct tctttattat ttggaatcac aactactaaa
     5161 tggctaataa caatttattc tacggctggt acatagtagc cgcatcactc attcttattg
     5221 ttcttgatgg acttcttctg tactcatttg gtattttcct tccgggcctg aacgataaat
     5281 ttggactgtc tgaagcaggc ggatcatcta tatatgcact caggtcattt gtgcttgctt
     5341 tttccctggc tatctccgga agactggtag ataagtatga tccaaggata attatattct
     5401 ctggcgggct tatctgtgca attggtcttc tgctctgcgg gtttgccgaa accaagtttg
     5461 agctgttctt gtattttggt gttcttattg gtattggtga cggggtttta tatataacct
     5521 gtgtaactct cataagcaga tggtttgtta agaaaagatc gcttgcaatt ggaataataa
     5581 caacaggagt accgcttagc ggtctttttg tccccccgat tacaacctgg ttaataatca
     5641 atcacggatt atcgaatgca ttttttggtc tagccgcatt tgtattcatc gctatttttg
     5701 ccgcactttt cttaagaaaa agccctgctg acatgaacat gaagccatat ggtgcagagg
     5761 atattggcaa agacggcaat aacacactta aatctcctga agaatctaaa gactgggaag
     5821 ctaaggaagc tatcaagacc ccggcgtttt ggctcttata cgtaatgtgg ctttttggtt
     5881 ttatcacttt tcttatcata atcactcacc tgttcaatta tgaaattcat gtaggcatcc
     5941 cggagctttt agcatcaggc gctccggcag caatcgggct tggaagcatt tttggaagaa
     6001 ttgtgctctc tgggttttta acagaacgaa tgcgaagcga gcgaatttta tttatatgct
     6061 tttttgtgca gagcagttcg ataattattt tgctactttt taaagatata gagtactact
     6121 ttattttcgg acttttattc ggatttttct acagcggctg ggtgccgata tttcctatga
     6181 ttctgggaga tttttttggc ctaaaagcac ttggtgcaat atacggtttt tttggaacct
     6241 cattttcaat tgcggcaatt atcggtccaa ttctggcggg ctaccttttt gacagaacag
     6301 gcagttattt ctatccgttt cttatagcaa taattatttg ctatctagca gcacttctta
     6361 catttttcat aaaaaaaccc gaacgaaaga tcaccgcctg atctccactt aggactaaac
     6421 atggaattca aagtagataa agactttacg gagtcagcag aaaaactact gggtagactt
     6481 aattaccaca agtatttctt tgattcaggc atcagcctgc aaagtaattg taaagacatc
     6541 ctggatgatc accgcgagtt attcagcccc gacgttcttg cagatctaga caggtctata
     6601 tataacgaaa atgaccatga aaaaatagag ctctttaagg tcttcgtctc aaacatgata
     6661 caaacctatt atggttcaga gcacattgat agattaaata atgaatttat caaacaagct
     6721 caaaatcctg agaataaagg caaaaacctt ttaaatctaa ttccagcaat taagaccgag
     6781 agaaaaagag aaaagagaaa caaattaggc aaacaatata gtgagcttag caaggagctg
     6841 taccataatc ttgagcttgt tatcgagaaa agaaatgaaa cttcaaccct gcttggatgt
     6901 aatgattata tagatcaaac tagaagttta tgcagcctgg atgtagaaag caccgaaaaa
     6961 ttatgcaatg attttttaag cgatactgaa tatatataca aggacttgct tggctggcaa
     7021 ctaaaaaaca agctggaaat aagaatagat gatgctaggc acgaagacat actattctta
     7081 tttaactcat atgagcttaa ggattatttc aaacaaacat cggcagacaa actatccacc
     7141 aggtttctgg atgaaatggg aataaattta cctggttcta tcaaatttga cctgaataat
     7201 agactcacaa aaaaccctga tgcgcgaacc tatcctgtat cagtaccgga taatatctta
     7261 atatcagtaa acagaataaa aactgctgag gactatgact ctatattggg gcaaattggc
     7321 aaatcactgc tccttggttc aataaatata gacgattcat ttgagaaaaa gaggttaatt
     7381 gatcctacta ctctagaaat attcaaattt ctcttcgaag atctgatctt tgaacaaaac
     7441 tggctgcaga gatatttaag acttgatact gacaaaaact ttttagaatt tctatattta
     7501 aaaaaactgt ttcagataag aaatctatgc ttaaatgtgc tgtcatacag gctgatatac
     7561 gaaaacaact tacaccaagc attagagcaa atatctgagt tatatcaaaa aagttctttt
     7621 ataaaaccaa atgaacatat ggtgctgctt aatttaattc tggacaaaga tactccgtat
     7681 tacagtttta gagcatgttt actagaatca tacttaaggg aatatcttat tagccgtttt
     7741 gatgaacagt ggtggagaga aatagatgca ggcagacagt tgataaaatg gtggaatcaa
     7801 aactcaggat taacaaccgg ttttttggag caagaaatag aaactggccc actcaatatt
     7861 aaaaaactaa tcaagtattt cgaaaaagcg ttttagcgta tatgcttttt taaacatatg
     7921 atacttttat tcacaacgac tctattaatt tgcctatctt tatatgcact attaactcta
     7981 tgtaaattaa catcaacatc tgaaaagatt gtagcggcag tgcttattat atcggctcaa
     8041 ataatattaa ctgagacgct tcttggtata atgaaaatac taactgccgg ctatttagtc
     8101 ttcattaact catctatttc tttagcatta ataatcttag tttataaaaa acaaaataat
     8161 atagttggcc tttttgctaa tgatgtaaat aagatctcac aatttcttaa gtcactcaat
     8221 tcaccctaca ttatattcct tttgatattg ctatgcttag ttagtttttg gttgttttta
     8281 tctgccttgc ttcttcctcc aaggggaatt gacgacctta catatcatct tccggcagtt
     8341 tatgaattta ttaatcatga aaaaatattt ctggtggcaa taaattatag aggtcatttt
     8401 gcttttccta tgaatgccga gctgttattc ctttggcccg tgatattcat aaaaaatctc
     8461 attccaatag gcttggtaca atatttttat ggtctttatg cagtagtagt tatttacgca
     8521 ctctccaaag agatgagact aaaaacacaa acctccatgt ttattggttt atgcttcttc
     8581 tttagccctg ttgtattggc tcaggccgga gtaggatata ttgatttaat agtaagtgtg
     8641 ttctatttaa ctgccatttt cttctcggtc agattttata gaacacaaag accaatatat
     8701 ttatatttat gcgctgtatc aattggtcta atagtaggga tgaaatatac gatgctcctt
     8761 tgcgcattag ttttgcaagt tctcatcctt cctggtttat caagaaaaaa gctcaagcaa
     8821 gcaggacttt atttattcat aataactatt ttctgctcat actggtattt aagaaatttt
     8881 ttaatacttg gacatccgtt ttatcctttt aatatatcgg gtcttgagag tacctacaca
     8941 agcttctctg acctgagcgt tttgaatata atttctaaat acttaaccat tctaaaactg
     9001 tttttcacca aagactgggg aattggctct tacaacggag gatatggaat aatattttgg
     9061 ggactggctt ttccaagctg gatatatttg tttgttaaat caatcatatc aaaaagtgaa
     9121 aacaggctga ttaatctatt tatgtggata cagatacctg ttggatttac attctttctt
     9181 atcgttccaa tcgacgaatt tcatctatat cccagatttt ccatattcat gatttctatt
     9241 ggattaattg cttttggtat ggttattgaa aagtttaata aaaaccagat ttataaaaac
     9301 gctgtacttt tttgcatctg tattttttgt tttgtttcaa catttcagct tgcaagggca
     9361 aagctcccgt cacatgcaat tgatgcaccc atttatgatt atataaacaa aaagaactat
     9421 tcaaatatca ggtacctgcg agtagcatcg acaagtgcat cttttctttg ggaaacatta
     9481 gacttcataa ctctaaataa tgagacagga ttagactgtt actttgcaag cagtcttctt
     9541 gattacgtag ggccaattta tggcagcaaa cttcaaaaca gggtgtggaa ctttaaagaa
     9601 gataaatcct cttcgcctga tgctcttata tttcttattc acgaggaaga caaggttaag
     9661 tattacggaa agaaaataac aattgatgag gttataaata atatagacta tgtattgata
     9721 gatattgctc aaaaagcgta tttgtatctg aacaaggatt ttttggataa agacagtgag
     9781 atacaaaaat cacttataaa acactatgaa aactactata ataaagaaat caaagtagca
     9841 tcgacactta ggaataaaat taacacagat actccaataa ttgcatcaga ttattatggt
     9901 ataggattac agtatctctc tatgacagag aacataagaa attcagtcct tatgcttccg
     9961 gagggtaaag aaaataagtt tttaggtgaa aatgttacag gaagtttcta tagtgttaat
    10021 aaaaagtata aaggctataa ctctaaacta gtttataaaa taagaattaa taaggatgaa
    10081 aatgtaatat tatatttaaa ctcaaaaaaa tgattagaaa gtttgagcat cacattttgt
    10141 ttatatttat tacacttata acatcggtac acctttacta tatgattggt cattacctgc
    10201 agggattttc aaggtttacc gagatctggc atgcggtata caaatttcta gggtttttaa
    10261 taatataaaa accctgtagt ctagtcgatt tgccggtgtt aataaaattc ctatgattta
    10321 aatttacgga ttcacaatca cctttccaaa ctgagctctg ttttcaatat gcttatgggc
    10381 tgctgcagtc tctgatagtg gaaacttctt atcaataact gactttatat atccgtttaa
    10441 tgtaagcttt aaagcctttt caagatttga tcttggtgca ttgaacaccg taactcccaa
    10501 cacttccaag tccttcatta taagcggtcc gaacattagc tcagctttta ccccgccaac
    10561 tactccaaca agaagaagcc tgccctttgc tgaaagtgac tgaatatttt tttcatatac
    10621 agaggctccg atctgatcaa atatgacgtt tacaccctta ccgcctgtca tattttttac
    10681 ttcttcagca aaatcctgcg tgttgtaatt gattatctca tcggcgccaa gctctttagc
    10741 tttttgcagt ttttcatcag acccggcggt agctattacc tggcttgcac caagatgttt
    10801 agcaatctga actgccatac aaccggttcc gcttcctgct gcatgtacaa gcactctctc
    10861 tccctcttta agccctgccc tttcaacaag cccataccaa gctgttactg tacaaatggg
    10921 agccgcagca gcatcgtcaa acgacagacc ttctggaatt gtgataacat tcacagcagg
    10981 caccttaaca aactcagcaa ggccgccgtg cctgtttacc ccaattactt caaacccttt
    11041 ttcaagcctt actgttgggt ctaccactac cctatcccca acattcaagt cccctgcatt
    11101 gccgcctacc tcggctatct ctcctgcaat atctattcct ccaatatgtg gcagatcaac
    11161 cggtcttggt gattttcccg atcttaacct cagatcaaga gcattaagtg aaactgattt
    11221 aacattaata agcacttcat catcagaaat ttgaggttta tcatactctt caagtttaat
    11281 tacatctgct tctccaaatt catggtatat ggctgccttc ataagattcc tccaagcttt
    11341 attaaattta aaagtgatat attatatagt ttttaaaaat taaaaaggtc tgtttataaa
    11401 aaattatatg tttggaatcg gaacgggtga aatattggtt attctggttg tggcgcttct
    11461 agtgcttgga ccaaatgaaa taccaaaagt cgcaagaact attggaaaga ccatgaaaga
    11521 cataaacagg ttcaaaaatg atttaaggca aagtgtagat accgaatttg aacagtatga
    11581 acagtctgag caaaagaaga aagaagagaa acctgacaag gaaaaagata aaatcacctg
    11641 ataataacca ttcagaacca ttaatacatc tactaaatga gtaagcttac cgagccagca
    11701 ccggttaaaa ttatattctc tgtaatcttt gcacggggcg ctgatacgga ttttttaaaa
    11761 caaatattaa tagaaaactt tggacagctg gattttgaaa gtcagataat aaattttgat
    11821 aaaacaggtt attacactga ggagatgggt gctgagcttt ttagaaaaat atatagtaaa
    11881 aaagaagttg taaagcgcga gcaaattata gatattaaaa ttaaagcaaa tgatatagaa
    11941 gaacaaacat ctgtaaataa taaaagagtt tttaacattg atcccgggta tttagcaaca
    12001 gaacattttg tattatcaac agcaaaaggt tatgctcatc gtccctacct gggaaaagga
    12061 gtatatgctg atttaaccct gatttacaga gataagaatt tcagtgaatt ggaatggact
    12121 tatccggatt acaaatcatc agaactgcag aatattctaa aagaaattag agtaaattac
    12181 attaaaaatt taagagagga aaaaatgtat gattagtatg acaggatttg gcagaacaga
    12241 gctgcagaca tcgcttggca ctgttgcaat tgaagcaaga tcagaaaacc acagatatct
    12301 tgatgtaacc acgcagctgc cggatatact ttctgagata gaaaacgaca ttcaggaaat
    12361 agtaaaaaag catatatcaa gaggcaagtt tagaatatat atatcagcaa agctaaataa
    12421 caaaagcagc catctggact acagccttgc aaaggactct ttaaataatc ttgaaaaact
    12481 taaaaaagac ctgaagttaa atggggatag gaagcttgaa caccttttga tgttaaaaga
    12541 cctctttacc actgaatcga aagaggaact aacaaaagct gatatcaaca aagtactaaa
    12601 tagccttaaa tcagtaatta aaaaagttca tgatgcaaga aaggctgaag gtaaatatct
    12661 taaatctgag cttctaaaaa gaataaataa aattgaaaag ctgctagcta aaataaaaac
    12721 tagaagaaag aactatccaa aagagacaaa agaaaagctc aaagaaagag taaacctgtt
    12781 attagaagat tctcagatcg aaaactcaag gcttgttcag gaaatcgcaa tactcgctga
    12841 aaaaagcgat ttcactgaag aaaccgtaag gttaaatgcc cacataaaaa gatttaaaga
    12901 cacgatgaat aagaacactc ccgttggaag agaacttgat tttcttattc aggaaatgaa
    12961 cagggaagcc ggaacaattt ctgcaaaatc aaaagacgca aagatatctc actttattat
    13021 cgaactcaga tcagaccttg aaaaaataag agagcagata cagaatgtgg aataggatta
    13081 tattaattca ggcagggtat acaggtcgaa tgctcacaat caattaaata atcataaatt
    13141 tgctttgcgg caagtgaatg cccatgtaca ttccagtgac cgtcgttttc aaaatgatat
    13201 ccaagaggat ttctaccttt ctcgaattca cttcttaaat caataaactc aatttcctta
    13261 tcgcttagca tagagatgag tttattatga acctcacttt caatttgctt attcttacct
    13321 acccaaagtc cgcgtgaagg aataattaca acgagcagat tatatttttt gtcgaggtca
    13381 cttaatatat gaacagagct atttaataca tttttatcaa tctcattttt agggattcca
    13441 tcgaggttat caataataag accatatcta acagctaaat ctcttagttc tgaattggaa
    13501 tgtacatatg ttgcaaaaag gttaaataga gcaaaattat atagaaaatg ttttattttg
    13561 ttatattttg aaggattgac aattctatta ctatttcgat ttccataatc cagcaaatca
    13621 ttttccatgc aaagcccaag tattacattt ttaattttag ctcctgtcga ctctgcataa
    13681 tttagtagtt cacggtaacc gtttaaatta tttgagatac tgatattgta tacaggttta
    13741 ttgataagtt tttgaagctg atcagaatat cttttatgct catcaacccc ccagcctagg
    13801 gaaaatgaat ctccaacaac tgccagttca tccggtttaa tttctgcaat atccttctta
    13861 tccctaaacc catatttgtt aaactctaca tatgtatcat aatcccctgt gttcttaatc
    13921 tgactgcccg aataattttt ctttccaatc cttatattaa attgctcgtt ataatcaaat
    13981 ttaatccttc ccgacgggtc gtattttgga tacatagttc ttatcccaaa ctcgcaggga
    14041 atcagaacag ttaatagaac tgaaataaaa actagaaatc taactatata ctttttaatt
    14101 atcatttaac taagctaatt gttttaaatc tactcaaaat taaacagtta attaaacatt
    14161 atatgttgac acaaaggcaa ttaacataaa aatataggcc aaatttattt gtggaggaac
    14221 aaaagttgga aaagacacta tcaattgtta aaccagacgg agtagaaaaa aatgtgatcg
    14281 gagaggtaat aagaagattc gaaagcgaag gaatgaaggt aataggcctt aaaatggtaa
    14341 aactctcaaa ggaacaggcg cagggttttt atgcagtaca caaagaaaga ccattttttg
    14401 acagccttac agattttatg tcatcaggcc cgtgcgtact aatggtactt cagggtgaaa
    14461 atgcaataaa gactgtaaga acaaccatgg gtgcaacaaa ccctgctgaa gcagataacg
    14521 gaacaatcag aaaagacttt gcttcagata ttgagcataa tattgtacac ggctcagatt
    14581 ccacagaatc tgccgactat gaaatcgttt atttctttaa tgacctggaa cttgcctaag
    14641 aatttgttta aagtttaact atggctaaca gaaaaaataa aagggcttat tctacagccc
    14701 tctttatttt tttagccgtt ctttgtctat acctattccg aaacagctac cttccggcag
    14761 caggaaacta tctagttatt caggatgacc tggaaaaagc agatgccatc tttgtgtttg
    14821 gaggatcggt tccaaacagg ataattgagg ctgccgagat atataaaaaa ggatatgccc
    14881 cgcttataat aatttcaaaa tatccaaggc ccgggggtta tgagtatctg aaaaataaag
    14941 gaatctccta cccggagggc catgatatta ataaatcgat tgctcagaag cttggaatac
    15001 caaagaagag tatagtcata acaaaagaca ggaacacgag cacatttgaa gaactggtaa
    15061 gattaaacaa gttttgtaag aacaataact ttaataaagt catcctggta agcaccaaat
    15121 cccataccaa aagaatctct aaaatattca cagatatatc tgagggtaaa ataaagggaa
    15181 ttgtcagata cacaaattac gacagttacg accctaaaaa ctggtggaaa gacaggagct
    15241 cactcaggca gacattgttt gaatatcaaa agcttttgca tcattttttt gttgatagga
    15301 agaaaattta atcaaattct atttcaaaaa tattttcaaa gttatctttc actatattaa
    15361 taatttcttt caattctact tctcttttta aaatttcaga tatggaagtt atttgtaaat
    15421 cggtaatacc acatggaact atatgtgaga atgcatcatc ctgtttagta atatttaaag
    15481 aaaaaccgtg catggtaatg tgctttttta cataaattcc tataaaggca attttggaat
    15541 taccaaccca tacacctgga tatttatctc tcctacatgc accaatttcc agggacctca
    15601 aagattgaat aatcacctct tcgagtaaat ttacatattt cttcaccccc aggccaagct
    15661 ctttaagatt caaaacagga taacaaacaa gctgcccggg gccgtggtat gtgaagtccc
    15721 ctcccctgtt tgtttcgaat agctctatac cccgctcttt taacactttt tgggttgcaa
    15781 gcaaattctg attacttcca aatttgccga ctgtaattac tggttcatgc tccagcatca
    15841 gtatatgacc atatgagcaa tctttaccgg aaacaatatc attttgcagt ctattctgaa
    15901 tatccagaca gtccgaatat gatatccttc caagcagtga ccagcttaca tttttattca
    15961 tattttttaa tttagattat ttattaatca ggttatatta atcaaaatgg caaaaacaac
    16021 caaaaacagt cttacatatt ctgagcttaa agacaggctt caaaaagaac tgctagatcc
    16081 tgtttttata ttacatggag acgatgagta tcttatagaa cagtctataa atgaaattaa
    16141 acagtctgtc gagcaaaaaa atcctgatgc cgattatctc tcttattatg cagattcagt
    16201 aaatatcacc gagattctgg attctgcaaa ctctatggga ctattttctg cagctaagat
    16261 tattgttata aaacaatgtg agaaacttct aaaagacgat gccggctatt tgatggaata
    16321 tattgataac ccaaacaagc agacatttat ggttttggtg tttgaaaatc ttaagaagtt
    16381 caaaatcaag ccaaatgaca atttcataag cgtaaatatc ccctcagact ccaaaaatat
    16441 catcttaaaa gtaaaaaaag aatcagaaaa gcagggttat catattaaaa acgatgctat
    16501 tttgaggctt acggagcttg ttggggaaaa tttgattgat attttaggtg aaataaaaaa
    16561 actctccata aatcagagcg gtgatataat tgaaaagtcc gatgttgaaa actatataaa
    16621 aaaatcaaag tttaaggatg tcttcgagct tataaatgca atttgcaata aagataaaag
    16681 caaagcctat gaaatattat cagagcttga gcaaaatggc gaagaaccca ttgctgttat
    16741 aaacagaatt tcctggcgaa taaggcagct atggcaggta aatcaactgg taaatgaaaa
    16801 aaaatccaag gatgagatac taaagacact caaaatttca tctggaaacc tctattatat
    16861 gcaaaaacag atgaaaaatt tcagaaatga agaatatata aggattttgg atactatcta
    16921 taatacggag cttaagctaa aatccactgc gacacccaga tttgaccttc ttacaagctc
    16981 agtgatgcag atgtgcagat agaatagttt gttatgttta aagattatgc ctgagcggca
    17041 gcttccagtt tatgaacttt ctttgagaag ttggaaatgt tccttgaagc tgttctctta
    17101 tgaattacac cctttgttgc agccttatca aaagctacgg tggtgtcttt caaaagctct
    17161 ttggattttt ccagatcctt actctctatt gtagaaagca gggttttaac cttggatcga
    17221 agagtagatt taatatgcaa gttcctctct cttcttacct tgctctgacg gtctcttttg
    17281 attcctgatt ttgatcttag tgccatgttt acccgcttta aaatttagtt ataattaaag
    17341 ggagcaatta ttagtctttt tattacagtt gtcaagtatt tattacttgc atttttatat
    17401 taactatgac ataatttacg actaaggtcg aataatatta gaaaatgaat atcaatttca
    17461 atagtactat taaatttcca tctgaagtaa ttattcagca aactgctgat gggcagagta
    17521 taatgcttaa tacaagaact gaaaattatt ttggactcga tgaaactggg acaatatttt
    17581 ataataacct aataaattca ctaaatataa ataaagcatt gcaaaagata cataatactt
    17641 atgaagtaga aaaagacacc cttagcactg atttgcaaag ttttattagt gatcttaaaa
    17701 aaaataagga gctcgcatag aacagagcta taagtaatgc agaatctcct ttattttatt
    17761 aactaaatct acatttctgt tattaaatct aaattccatc tcttttagat acaaaacaaa
    17821 gtgttttc
//
LOCUS       DBNGSV010000202         8732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1697867, whole genome shotgun sequence.
ACCESSION   DBNGSV010000202 DBNGSV010000000
VERSION     DBNGSV010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1697867"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC159U_07345"
     CDS             <1..263
                     /locus_tag="AC159U_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HIR3336911.1"
                     /translation="DIEFDYIVTVCDSAQEKCPHFGGSGIKIHKSFNDPSAYSGSDEE
                     KAGFFRKVRDEIRGWIDNEFINYTNPIQNVPTAAKEQVNDQV"
     gene            250..918
                     /gene="phoU"
                     /locus_tag="AC159U_07350"
     CDS             250..918
                     /gene="phoU"
                     /locus_tag="AC159U_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3336912.1"
                     /translation="MIKFKEELNNLIKLMLNMASVAEEMITKSTKALVDRNMILAEEV
                     IKMDQRVNEMEIEIDNLCIKIQALYQPEAENLRTVTMILKINNDLERIGDHAVNIAEK
                     VIFLSDKPPVKPLIDIPRMADKSVEMLRDSLKAFIEKDSELAVQVCKKDDEVDILEKQ
                     VMRELISYMVSDPRTIDRALGLIFIARDLERVADLATNISEDVYYIVHGKNLKHHALE
                     KGSA"
     gene            complement(921..1751)
                     /locus_tag="AC159U_07355"
     CDS             complement(921..1751)
                     /locus_tag="AC159U_07355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3336913.1"
                     /translation="MRKYLKSLAILAFVIILSGFSVISCTQAPVTGRSQFILLSEDQE
                     IEMGLVAFQDVLKKENISKNTKYNSSVTRVGKRIAQASGKTGYKWEYKVIEADDTINA
                     FALPGGKIAVYTGILKVAETEAGLATVMGHEVAHATARHGGERISVGILAQLGAVGLN
                     VALRNKDPQTINAVNAAFGTGVTLGGILPFNRKQESEADQIGLIYMAKAGYDPRESVN
                     FWKRMAKASSGKPTPPEFISTHPSHGTRITNLNKWIPGAMEIYKKSPKASSQQITLNK
                     "
     gene            complement(1872..3044)
                     /locus_tag="AC159U_07360"
     CDS             complement(1872..3044)
                     /locus_tag="AC159U_07360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3336914.1"
                     /translation="MSYPFSSRLSHFVPPKQWASMRVARDLEKKTGKKIIHFEKGDYQ
                     GPDFDTPQHVLDATEQALRDGYVRYDPGPGLPELREALAERMLRRGRKTQPEEVIVTA
                     GAKHALTMSLLTFLEDGDEVIFPNPGYPPDEVWAKYVRANIKHTPLIQENWQFDVDKL
                     ESLITPKTKMVIINTPQRPNGHLVDNPEKIAEVILKHKDLMVITDEIFSEVVFDGKKH
                     KSISGIEEVKDRCIVIDTFSKTWAMTGWRIGWAVAPKAVTDKLSIFLQDSITNVAAFI
                     QKAAYSAITGPQDWVDNKLQLLQDKRDRMVAGLNTIDGIKCDTPDGAFYAFADISGTG
                     LTSQEYTDRLVERAGVAVVAGTAFGSQGEGYVRITYACSDADIDEGIKRMKEADLS"
     gene            complement(3076..3732)
                     /locus_tag="AC159U_07365"
     CDS             complement(3076..3732)
                     /locus_tag="AC159U_07365"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3336915.1"
                     /translation="MAKAARKSMAMKNKSAKKLNKKEDIIKAAAKLFSQKSFHDVKMD
                     EIAEELGIAKGTIYLYFKSKEKLYLEILEESYEAIEALLEKEIANDEPSTLKLKKVLG
                     IIFSFYRRNLDVLKILSRDETHLIKEHFEFTEHWRLRRLKLYEKILEKGIKEGSFNVQ
                     NPKLTSLIIFGLVRSVMFFYSTEKNTTDIAEEVFNVITNGILNSKLNEYEKGLKVINH
                     "
     gene            complement(3898..5430)
                     /locus_tag="AC159U_07370"
     CDS             complement(3898..5430)
                     /locus_tag="AC159U_07370"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HIR3336916.1"
                     /translation="MESLLLFSKITLSSLYIFILVVLCILGLNRYYLFYIQNKFKNKK
                     NRPLKYYSRLPRVTVQLPIYNEMYVAKRLIDAVCKLDYPSDLLEIQVLDDSTDNTSVI
                     AENAVKSHSSEGVNIQYLHRDNRAGFKAGALENGLKYSRGELIVIFDADFIPPADFLT
                     KTVDYFTDENVGIVQTRWGHINKNYCSLTKAQSILLDGHFVVEQFARSNGGYYINFNG
                     TAGILRKSCIKDAGGWQHDTLTEDLDLSYRAQMKGWRAVYLNDVVVDAELPVEINSFK
                     SQQHRWAKGGIQTAAKLLPSLLSNKNIQTRVKIQSLFHLLGNISYPLLLMLIILMIPL
                     AYLWSSVGWEKVIMFNLLAITAGTLSVFNFYFTAVRSVYGSNWLGFARYIPLSIALGS
                     GLAINNTKAVFEALFKRDTDFNRTPKFAVTKNSDTYFKKNYTTSRNFTSLIELLLGIA
                     LMIKTVYAAYLGFYGWIPFLLIIQFGFFYTSLLSYFHNNINIFDTVKPTILKKLTLLR
                     SK"
     gene            complement(5457..6623)
                     /locus_tag="AC159U_07375"
     CDS             complement(5457..6623)
                     /locus_tag="AC159U_07375"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010285 -
                     L,L-diaminopimelate:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR3336917.1"
                     /translation="MEINWADRINELPPYLFAEIDAKKAEVMAKGVDVIDLGVGDPDI
                     PTPNFIIEALNKAVRDPSNHVYPSYAGMLSFKQAVSDWYKKRFNVDLEAASEVVTLIG
                     SKEGIAHTPLAFINPGDIALVTDPGYPVYNVAITFAGGVPYSIPILKENNFLPDFDTV
                     PEEILKRSKLLFINYPNNPTTAVADEDFYKRVIEFAEKNQIIVCHDAAYTEIAYDGYK
                     PLSFLEIDGAKEVGIEFHSLSKTFSMTGWRIGFAVGNKKAIAGLGKIKTNIDSGVFQA
                     VQHAGIEALNNYTEGLADRVNIYKERRDIFCKGLDEIGISYLHPKATFYVWFETINGL
                     SSKDFASKMLDEAGIVATPGNGFGEYGEGYMRVSTTFENSKIVEAVERLKNLKL"
     gene            complement(6738..7871)
                     /locus_tag="AC159U_07380"
     CDS             complement(6738..7871)
                     /locus_tag="AC159U_07380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3336918.1"
                     /translation="MKKLENKIISYIQGNGPITFDKFMEFALYDKEDGYYCKSGKRFG
                     KSGDFYTSPTVHRAFGNVISKFIVRVFEAIDEQFVSVIEPGCGNGYLAVDILDCLKEF
                     YPTVYENIHYYCIDKSISNLDNAYELLADHRAIVNFAADISDLKDKHSGVVISNELFD
                     ALPFHRIKFKDNKPYEIYIENEGESFTEHLGKLSSNDLQKFIDNLNIHFEQDQQIEIN
                     LNYKKVIEQISNSLNKGVVVTFDYGWLSGELYSPERLDGTYRCFHNHQISADPYINIG
                     KQDITSSVNFSDLIKLGEQNELKLIKYSTQGQFMVDWGVLDIIEKLDEKGRSTAKNLF
                     MPGTMGDMFKVLIQQKNMNDSFTEHYPESILKISYQSTKDTIR"
     gene            7929..>8732
                     /locus_tag="AC159U_07385"
     CDS             7929..>8732
                     /locus_tag="AC159U_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336919.1"
                     /translation="MPLFSDAPDDWPQLDIKLQVGNNHINAINIAKELTNDFKENKKD
                     YIVSSFLESLLLENGVLQSHITISHPDGKYYVFIFHTDSALSSRFYAGIKYLFSNSKT
                     TKCIYFSSFELDSDAKPALPLREFAPDLFSTLKTSIPEDTYCIWSSEGQEVKFAQSKE
                     YLHIDELAQLSDGIHSYLLAEILRSINEIEQDVGRIELPDEEFSTVLVGPENKVILLS
                     ASKKRGVMFHFGEDQTTSRYRTLFLKQFNTYVKGLRDYIAEKEVELDSYS"
BASE COUNT         2586 a         1659 c         1536 g         2951 t
ORIGIN      
        1 cagatattga atttgactac attgttactg tttgtgatag cgctcaagaa aaatgtccac
       61 attttggtgg ttctggaata aaaattcaca aaagttttaa cgatccatca gcttatagtg
      121 gaagtgatga agaaaaggcc ggattttttc gaaaagtaag agatgaaata aggggctgga
      181 tagataatga atttataaat tatacaaacc ctatccaaaa cgtccctact gcggcaaagg
      241 agcaggttaa tgatcaagtt taaagaagaa ctgaataatc ttataaagtt aatgttaaat
      301 atggcctctg ttgccgagga aatgattact aaaagcacaa aggctcttgt tgacagaaac
      361 atgattttgg ccgaagaagt gataaagatg gatcagcgag ttaatgaaat ggagattgaa
      421 atagataatc tctgtataaa gattcaggca ctttatcagc cggaagccga aaatttaaga
      481 actgttacaa tgatactgaa aataaacaac gaccttgaga gaattggaga ccatgcggtt
      541 aatatagcag aaaaggtaat tttcttatcc gacaaaccgc cagttaagcc tctaatcgat
      601 attccccgga tggcagataa atctgtagaa atgttaagag acagcttaaa ggcattcata
      661 gaaaaagatt ctgagcttgc agtgcaggta tgtaagaaag atgatgaggt ggatatactt
      721 gaaaaacagg tgatgaggga gcttatttct tacatggttt ctgatccaag aaccattgac
      781 agggcgcttg ggctcatatt tattgcacgt gatttagaaa gagtagctga ccttgctact
      841 aatatttctg aagacgttta ctacatagtt cacggcaaaa atcttaagca tcatgcgctt
      901 gagaaaggaa gcgcataaaa ctacttatta agagttattt gctgactgct tgcttttggt
      961 gattttttat atatttccat ggctcccggg atccatttgt taaggtttgt tatcctggtt
     1021 ccgtggcttg ggtgagttga aataaattcg ggaggtgttg gttttccgct gcttgctttt
     1081 gccattcttt tccagaagtt tacagattct cttgggtcat aacctgcctt agccatatag
     1141 ataagaccaa tctgatcggc ttctgattcc tgttttctgt taaaaggaag tatcccgcca
     1201 agtgttacac ctgttccgaa tgcagcgttt actgcattta ttgtctgcgg gtctttgttt
     1261 ctgagtgcta cgtttagccc aaccgctcca agctgagcaa gaatacctac agatatcctc
     1321 tcaccaccat gtcttgctgt tgcatgggct acttcatgac ccataactgt tgcaagcccg
     1381 gcttctgttt cagctacttt taatattccg gtgtatactg cgatctttcc acccggaaga
     1441 gcaaaagcgt ttatagtatc atcagcttcg attaccttat attcccactt gtaaccagtt
     1501 tttccggatg cttgcgctat tctcttacca actcttgtta cagagctatt gtattttgtg
     1561 ttttttgaaa tattttcttt cttaagcaca tcctgaaagg ctaccaatcc catttcgatc
     1621 tcctgatcct cagaaagcag tataaattgc gatctgccgg ttacaggagc ctgtgtacag
     1681 gaaattacgc taaatcctga caggattatt acaaatgcta ggattgcaag tgatttaaga
     1741 tattttctca ttttattatt ataaataata tgttctatat attaaactgg aaaatttaaa
     1801 aaaagttata ttccaaataa tatatgagag gcccttaatt aataagaacc tctcatttga
     1861 ttttaattgt cttaactcaa atcagcttct ttcattcttt tgataccttc atcaatgtct
     1921 gcgtcagaac atgcataggt tattcttaca taaccttcac cctgacttcc aaatgcggta
     1981 cctgctacta cagccactcc ggctctttca acaagtctgt ctgtatattc ctgtgatgta
     2041 agacctgtgc cgctaatatc tgcaaatgca taaaaagcgc catctggtgt atcacactta
     2101 attccgtcta ttgtgtttag acctgcaacc attctgtctc tcttgtcctg taataattga
     2161 agtttgttgt ccacccagtc ctgaggtcct gttatggcag agtaagctgc tttttgaata
     2221 aaagccgcaa catttgttat tgaatcctgc aggaatattg agagtttatc tgtaactgct
     2281 tttggtgcta cagcccagcc tattctccat cctgtcatag cccaggtttt cgaaaatgta
     2341 tcgataacaa tgcatctgtc tttcacttcc tctatacctg agattgattt gtgtttctta
     2401 ccgtcaaaca ctacctcaga gaaaatttca tcggtaatta ccattagatc tttatgcttt
     2461 agtattacct ctgcaatttt ttcgggatta tctacaagat gaccatttgg tctttgagga
     2521 gtgttaataa taaccatctt ggttttagga gtgatgaggc tttcaagctt gtccacatca
     2581 aactgccagt tttcctgaat gagcggagta tgtttaatat tggctcttac atattttgcc
     2641 catacttcat ctggcggata tccggggttt ggaaatataa cttcatctcc gtcttctaaa
     2701 aatgtgagaa ggctcatagt cagcgcatgc tttgcgccag ctgtaacaat tacttcttct
     2761 ggttgagtct tccttcctct tctgagcatt ctctcggcta gagcttctct taattcggga
     2821 agtcctggtc cgggatcata tctcacatat ccgtctctaa gtgcctgctc tgtcgcatct
     2881 aaaacatgct gaggcgtatc aaagtcgggg ccctgataat ctcctttttc aaaatgaata
     2941 atcttctttc cggttttctt ttccagatca cgagccacac gcatactggc ccattgtttt
     3001 ggtggaacga aatggcttaa tctgctgcta aatggatacg acattctcat atacctccat
     3061 atatgaatta atcatctaat gatttattac ttttaatcct ttttcatatt cgtttaattt
     3121 tgaatttaat attccattgg taataacgtt aaaaacttct tcggcaatat cggttgtatt
     3181 cttttcagtg ctgtaaaaaa acattactga acgaaccagc ccaaagatga taagtgaagt
     3241 aagttttgga ttctgaacat taaaagagcc ttcttttatt cctttttcca gtatcttttc
     3301 ataaagtttc agtcttctta gtctccagtg ctctgtgaat tcaaagtgtt cttttattag
     3361 gtgtgtttcg tctctgctca gaatcttaag tacatcaagg tttcgtctgt aaaaactaaa
     3421 gattattcca agtacttttt ttaactttaa agtggagggt tcatcatttg caatctcttt
     3481 ttctaataga gcctcgatag cttcatagct ttcttctaaa atttcgaggt aaagtttttc
     3541 ttttgatttg aaatagagat aaatagtgcc cttggctatt ccaagctctt cagcaatctc
     3601 atccattttt acgtcatgaa aactcttttg agaaaatagt tttgcagcgg ccttaataat
     3661 atcttctttt ttattaagtt tttttgcgct tttatttttc attgccatac tttttcttgc
     3721 ggcttttgcc atttatttac accccaaaaa actgactagt cagtcatcct agtatatcct
     3781 tcattcattg tcaagtgttt ttttatctat ttggataaat tgaatatgta gggaattaaa
     3841 gcgcagttag atagtgtgtg taactaaact gtattgttaa taaactatat tgctatgcta
     3901 ctttgatcta agaagggtca actttttcag tatagtaggt tttactgtat caaaaatgtt
     3961 tatattatta tgaaaataag ataaaagaga agtatagaaa aatccaaatt gtattattag
     4021 cagaaacggt atccatccgt aaaatcccaa atatgctgca tagaccgtct ttatcattag
     4081 ggctatccca agcaaaagct caattaatga agtgaaattc cgggaagtcg tatagttttt
     4141 cttaaaataa gtgtcactat tttttgttac agcaaatttg ggggttctat taaaatctgt
     4201 atctctttta aaaagtgctt caaatacggc ttttgtattg tttatagcca gacctgatcc
     4261 aagggcaatg gatagaggta tgtatcttgc aaatccaagc caattggagc cgtacacact
     4321 tcttacagca gtaaaataga agttaaaaac actaagtgtt ccagctgtaa ttgcaagcaa
     4381 attaaacatt attacttttt cccatcccac tgaactccaa agataagcca gcggtatcat
     4441 aagtataata agcatgagta gaagagggta cgaaatgttt cccaataaat gaaacaagct
     4501 ctgaattttt actcttgtct gaatattttt atttgaaaga agcgaaggaa gtagcttggc
     4561 cgcagtttgt attccgcctt ttgcccacct gtgttgctga gatttaaaag agttaatttc
     4621 tacaggaagc tcagcatcaa ctacaacgtc gtttagatac acggctctcc agcctttcat
     4681 ctgtgccctg taactaagat caaggtcttc agtaagtgta tcatgctgcc atcctccggc
     4741 atctttaata catgattttc ttagaattcc agctgtaccg ttaaaattta tataatatcc
     4801 accattagat cttgcaaact gctcaaccac aaaatggccg tcaaggagaa tggactgagc
     4861 ttttgtgagg gaacagtagt ttttgtttat atgtccccag cgtgtctgaa ctataccaac
     4921 attttcgtca gtaaagtaat ctactgtctt tgtaagaaaa tccgccggag gaataaaatc
     4981 ggcatcaaaa atcacaatta gctcacctct tgaatatttc aggccgtttt ctaaggctcc
     5041 ggctttaaac ccggctctgt tatccctgtg taagtattgt atattaactc cctcagaact
     5101 atgagatttg acggcatttt cagcaattac cgaggtgttg tccgtagaat catcaagcac
     5161 ctgtatttcc aataaatcag aaggataatc cagtttgcaa accgcatcta taagcctttt
     5221 tgctacatac atctcattat aaatcggaag ctgtactgtg acccttggaa gtcgggaata
     5281 atattttagt ggtcggtttt tcttattctt gaatttgttt tgtatataga aaagatagta
     5341 tctgttaagc cctagaatac acagtactac caatataaag atatataggg atgataaagt
     5401 gattttagag aataagagta agctttccaa tgtgaatctt taaaggattt attttattaa
     5461 agttttagat tcttaagtct ttcaacagcc tcaacaattt tagagttttc aaatgtggta
     5521 gatactctca tatagccctc accatactca ccaaacccgt ttccaggagt tgctacaatt
     5581 cctgcttcat ctagcatttt tgaagcgaaa tcttttgatg acaggccatt tatagtttca
     5641 aaccacacgt aaaatgtagc ttttggatgt aggtagctta ttcctatttc atcaagcccc
     5701 ttgcagaaga tatcccttct ttctttgtaa atgtttaccc tgtctgcaag tccttctgta
     5761 taattgttca aggcctctat tcctgcatgt tgcacggcct gaaaaacgcc tgaatctata
     5821 ttagttttta tctttccaag cccggcaatt gcttttttat ttccaactgc aaatccaatt
     5881 cgccacccgg tcatgctaaa tgttttagat agcgaatgaa actctatacc aacctctttt
     5941 gccccatcta tttctaaaaa actcaaaggt ttatacccat cataggcaat ctcggtgtaa
     6001 gcggcatcat gacacacgat gatctgattt ttctctgcaa actcaattac tcttttataa
     6061 aaatcttcat ccgctaccgc tgttgtaggg ttattgggat agtttataaa cagcagcttc
     6121 gatcttttta gtatttcttc gggaacagta tcaaagtcgg gtaaaaagtt attttctttc
     6181 aggataggta tagaataagg tacgccaccg gcaaaagtga tagctacatt gtataccggg
     6241 tatccggggt ctgtaacaag tgctatatcc cctggattaa taaaagctaa gggggtgtgt
     6301 gcaatgcctt cttttgaacc aataagcgtt acaacctcac tcgcggcttc taaatctacg
     6361 ttgaaccttt ttttgtacca atctgaaact gcttgtttaa aagacaacat acctgcataa
     6421 gaagggtaga cgtggttcga agggtctctt acagctttat taagcgcttc gattataaaa
     6481 tttggtgtag gaatatcagg atcaccaaca cccagatcga ttacatctac gccttttgcc
     6541 ataacttctg ctttttttgc gtctatttca gcaaagaggt aaggtggaag ttcgtttatt
     6601 ctatcagccc agtttatttc cattgaaatt ctcctaaatt aattaaaaat tggttataag
     6661 gagattatct taacctaaca aaaatttatc cacaaggtct tattaatata tagcatgtgg
     6721 taaattatta ataattccta ccgtatcgtg tctttagtac tttgatagct tatttttaaa
     6781 atagattcag gataatgttc agtaaaactg tcattcatgt ttttttgctg aattaatact
     6841 ttaaacatat cacccattgt gccaggcata aaaagattct ttgctgtaga gcggcccttt
     6901 tcatccaact tttcaataat atccaaaaca ccccagtcaa ccataaattg tccctgagta
     6961 ctgtatttaa ttaactttaa ttcgttttgc tcaccaagtt tgattaaatc actaaagttt
     7021 acgcttgatg taatgtcctg ctttcctatg tttatatatg gatcagccga tatttggtgg
     7081 ttgtgaaaac atctgtaagt accatctagt ctttccgggc tataaagctc accagaaagc
     7141 cagccgtaat caaacgtgac tactactccc ttgtttaagg agttagatat ttgttctatc
     7201 acctttttat aattcaaatt tatctcaatc tgctgatctt gctcaaaatg tatatttaag
     7261 ttatcaataa atttttggag atcattgctg ctgagcttac ccaagtgttc ggtgaaactt
     7321 tcaccttcgt tttcaatata aatttcatag ggtttattat ctttaaactt gattctgtga
     7381 aatggcaggg catcaaaaag ttcatttgat ataacaactc cactatgttt gtcttttagg
     7441 tctgaaatat cagctgcaaa attaactatt gctctatggt cagcaagcag ttcataagca
     7501 ttgtctaaat tcgatatgct tttgtctatg caataataat ggatattttc ataaacggtg
     7561 ggataaaact cttttaagca atccaatata tctactgcca gatacccgtt tccacagcca
     7621 ggctcaatca cagatacgaa ttgttcatct atggcttcaa aaactctgac tatgaatttt
     7681 gaaataacat taccaaatgc cctgtgcaca gtggggcttg tataaaaatc tcctgatttt
     7741 ccaaaccttt tgcctgattt gcagtaatat ccatcttctt tatcatacag ggcaaattcc
     7801 ataaacttgt caaatgttat tgggccgttt ccctgtatat aagatataat tttattttcc
     7861 agctttttca ttgtttaagt acgaaccatt atataatata tgaatactta aattacagga
     7921 gtatgacaat gccacttttt tcagacgcac cggatgactg gcctcagttg gatataaagc
     7981 tgcaggtagg aaacaaccac ataaatgcta taaacatagc aaaagagctt actaacgatt
     8041 ttaaggaaaa caaaaaggat tatattgttt cctcatttct agaaagtctg ctattagaaa
     8101 atggagtact acaatcacat attacgattt cacatcccga cggtaaatat tatgtcttta
     8161 tctttcatac cgatagtgcc cttagcagca ggttttatgc tgggataaaa tatttatttt
     8221 caaactctaa aactacaaaa tgtatttatt tttcaagttt tgaattagac tccgatgcta
     8281 aacctgctct tcccttaaga gaatttgcac ctgacctctt ttcaacacta aagacttcga
     8341 tcccggaaga tacatattgt atatggtcat cagaaggcca agaggtgaaa tttgcccaat
     8401 caaaggaata tttacatatt gatgaactgg cgcagctttc agatggtata cacagctacc
     8461 tgttagctga gatattaaga tcaataaacg aaattgagca agatgttggc aggattgagc
     8521 ttcctgatga agagttttct actgtacttg ttggccctga gaataaggtg atattattaa
     8581 gtgcatcaaa aaagcgtgga gttatgtttc attttggtga agatcagacc acgagcagat
     8641 acaggacatt atttctaaag cagtttaata catacgtaaa agggttaagg gattatattg
     8701 ctgaaaaaga ggttgagctg gacagttatt ct
//
LOCUS       DBNGSV010000203        15410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_173458, whole genome shotgun sequence.
ACCESSION   DBNGSV010000203 DBNGSV010000000
VERSION     DBNGSV010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_173458"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC159U_07390"
     CDS             <1..306
                     /locus_tag="AC159U_07390"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR3336920.1"
                     /translation="DVAMTGEITLRGKVLPIGGLKEKILAAHRARVTQVIIPKDNLKD
                     TKDIPSNILKDIEIKLVEHMDEVLKEAIISDTEILNDKIDSTQIDITNSETHRITHS"
     gene            412..882
                     /locus_tag="AC159U_07395"
     CDS             412..882
                     /locus_tag="AC159U_07395"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR3336921.1"
                     /translation="MQVKHIPIDKQKRIALVAHDNKKEILLDWAKKNKDVLIKHNLFA
                     TGTTGKLIQDTLGVKVKRFNSGPLGGDQQVGAKIAEGKLDLLIFFWDPLSPQPHDPDV
                     KALLRIAVLWNIPTACNRSTADFMISSSLINKAYDRIVIDYSKYKQRFNNQSIN"
     gene            886..1128
                     /locus_tag="AC159U_07400"
     CDS             886..1128
                     /locus_tag="AC159U_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336922.1"
                     /translation="MKEQLEETINTANNGNWEKAHKIVQDIEHELAYWIHANLHREEG
                     DVSNSKYWYKRAGREFTEMDLEEERRLIKEEIENKL"
     gene            complement(1135..1632)
                     /locus_tag="AC159U_07405"
     CDS             complement(1135..1632)
                     /locus_tag="AC159U_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR3336923.1"
                     /translation="MKVDVGIEIMVRAFNIHWGRKRKVIKYFAYSLAVTIIAFGIWII
                     SKNYSNSYQGYISVFIGITAILLINLNNSIIRWKLNKQYNQMGDDIQNVDMSFDKENL
                     YVKTPKSETKIRWDTFVKWKERDGLILAYRNDSYFQVIPVSQFPENDGLEIRSLLDEH
                     STMIV"
     gene            1728..2588
                     /gene="ypfJ"
                     /locus_tag="AC159U_07410"
     CDS             1728..2588
                     /gene="ypfJ"
                     /locus_tag="AC159U_07410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR3336924.1"
                     /translation="MRWRLGRRSSNVEDRRGRRVGRKSAGGGIGFIIILLIGMYLGID
                     PSTLMNIKSGIDSVSVQEQNTQTTNPGVKDELSDFVSVVLADTEDTWNPLFKQMGGNY
                     REPRLVLFSGAVKSACGLAQFAMGPFYCPRDQQVYIDLSFYQDLKHKLGAPGDFAQAY
                     VIAHEVGHHVQNLLGISEQVYNARTRMSKVEGNKLSVMQELQADCFSGIWAHHADKAR
                     QILEKGDIDEALNAASSIGDDRLQKQSTGHVTPDSFTHGSSAQRVRWFKIGLKSGDIN
                     SCNTFEADRL"
     gene            complement(2599..3504)
                     /locus_tag="AC159U_07415"
     CDS             complement(2599..3504)
                     /locus_tag="AC159U_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336925.1"
                     /translation="MSVFVFLSSLLHDHNTKFFKDVKIFTNTLHLRPVGEIDNDELQN
                     KNNVYKLYEEKHNHYIEHYGTKNIFGANPILNKFKVTRDKVLLNRTKKQEIINTEFGS
                     INSSTKYIYKGRRPHDLIRFNVKVANDDLHADIPEILDEFEDTISLKLDAISLIRIFD
                     GLEDQKLIVSAVYHPSDLSIYCFNVFATVNGRVVDNDHVLLPNEYIRKGSCKTDFDSS
                     AKYFGFPLVLNYGDYIEKPMHIRGASNLKVKLELVNLHYNHNYIVRRYIKNKNKPIET
                     SWLKFQKTKEDIALEEFDKLKKFGK"
     gene            complement(3647..4114)
                     /locus_tag="AC159U_07420"
     CDS             complement(3647..4114)
                     /locus_tag="AC159U_07420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3336926.1"
                     /translation="MSDKPFQDYWKHNKCYGCGHNEHGHKIKSHWDGDKSVCIWKPEE
                     YHAAGPPGFLYGGIISSIIDCHTVCSAIAYEYNIEEREYDSKPLIWCVTASLKVDYLK
                     PTPIDKPVKLEAYIQKREGRKIWLSCDMFSDDLKTATGEVLAIRVNADDWYVE"
     gene            4389..5534
                     /locus_tag="AC159U_07425"
     CDS             4389..5534
                     /locus_tag="AC159U_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3336927.1"
                     /translation="METNLTDDQKMIRELISDYSEKEIKPEASENERNSRFPSELIAK
                     LGELGFMGHFVPTEYGGSGLDYLSYIIAVEEISKACASTGIIVSAHNSLACSPIVYFG
                     TEQQKQKYLTDLASGTKLGCFALSEPEAGSDATSIKTTAVKKEKHYIINGLKSWITNG
                     SEADVAIVFASTDKDKGSRGISAFIVDLDSEGIEIGKSEHKLGIKASSTTQIIFDDCI
                     VPAENLLGEEGEGFKIAMKTLDGGRIGVAAQAVGIARAALEASIIYSKQRKAFNDTIS
                     NFQGIQFMLADMSTKVEAARLLTWQAASLLDQGEKFTKQSAMAKLFAAETAMWVTTKA
                     IQIHGGNGYTTDFPVERYFRDAKVTEIYEGTSEIQKIVIARQALNEY"
     gene            5553..6167
                     /locus_tag="AC159U_07430"
     CDS             5553..6167
                     /locus_tag="AC159U_07430"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3336928.1"
                     /translation="MQNEVQSKIKNKKLIDTRRTELINAAVKLFVIKGFHETSVREIA
                     KESGMSMGALYDYIRTKNDILFLVCDHIHSSISTKLKESVASNKPPIDILKNAIKDYF
                     KIIDKNQNNMLLLYQETKSLSKSARKYIFKAESDLTSIFKDILIQCIKDKSVSISKKK
                     ADIVSHNIMVAGQMWAFRRWALSENYTINSYINSQTELLLNGVK"
     gene            6182..7024
                     /locus_tag="AC159U_07435"
     CDS             6182..7024
                     /locus_tag="AC159U_07435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3336929.1"
                     /translation="MNMVGIVGSGTMGSGIAQLVAASGYEIVLIDLNDELLKKSVSSI
                     NKNLSRLVDKEKITKEIHQQILKRVNTSTNLDNLSSCDLVIEAVSENIDIKRELFVKL
                     NAVVKKDCIIASNTSTLSITELAMFTSRPDKVVGIHFFNPAPVMKLVEIIKSLATSKQ
                     TIKTAEEFILSLDKDPVVTKDRPGFIVNRILVPMLNEAVFALDEQMGTAEDIDKAMKL
                     GTNHPMGPLELIDLIGLDVTLDIMDVLFAEFKDSKYRACPLLRQKVRAGHLGRKSGRG
                     FYDY"
     gene            7042..7824
                     /locus_tag="AC159U_07440"
     CDS             7042..7824
                     /locus_tag="AC159U_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3336930.1"
                     /translation="MSENIIYKKIKPNIGLLTINREKALNTLSLETVTELKEFIDNQL
                     PKEEIRVLIITGSGKKAFVAGADISQMKEMNKDEFKNYCEISHGNFNNLQKLIIPVIA
                     AINGYALGGGSELALACDIRIASDNAKLGFPETKLGLFPCWGGSQRALRLLGIAKAKE
                     LIFTGEMISAEQAAEIGMVDKVVKQDDLMDEVVSIAEKISGNSPLAVGYAKEMINSGQ
                     DLVLSESLELELQKGIECFDSYDRQEGMSAFIEKRTAEFKGT"
     gene            7826..9019
                     /locus_tag="AC159U_07445"
     CDS             7826..9019
                     /locus_tag="AC159U_07445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3336931.1"
                     /translation="MEKVVLCNPLRTAIGTFGGTLKDTSAVDLGITVVKEILNQSSLD
                     PKNIDDCIMGNILGAGLGQNPSRQIAINSGLPVETPALTLNRLCGSGLQSVIFAAQAI
                     KAGDADCIVAGGIENMSQAPFYLKKARWGYKMAMPKEEIIDGMVYDGLWDIFGDYHMG
                     ITAENLAEQYNISRERQDEFAYNSQMKTKQAQEDGKFDSQIVPISIPKRSGEYTKFKT
                     DEHPRGDVTIESLNKLKPVFKKDGTVTAGNASGINDSAAAIIVCSESKASELDLEPLA
                     FIRSYTLAGVDPSIMGIGPVPAIQKALTKANLSIDDIDLFELNEAFAAQSLACLSDLP
                     IPENKLNVNGGAIALGHPIGASGAVILVKLLHELKQRKEAKLGLCSLCIGGGMGIAMV
                     VEKIK"
     gene            complement(9055..9897)
                     /locus_tag="AC159U_07450"
     CDS             complement(9055..9897)
                     /locus_tag="AC159U_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3336932.1"
                     /translation="MDTKPENNSISTTRFKLHEIIFEADTFLGKIFDIVLISCIVLSV
                     IFVMLDSVRSFREQYGNLLYLAEWFFTIVFTIEYVLRLYCVGNRIKYAISFFGIVDLL
                     AIVPTYLSLLLPGSQYFLVVRLLRILRIFRVLKLAHYVGESTLLLQALVASRRKIFVF
                     LMTVLTLVIILGSIMYLVEGEKNGFSSIPKSIYWAIVTITTVGYGDVAPVTNLGKFIA
                     SIIMIIGYSIIAIPTGIVTVEIANAFKGNLSTQVCLHCNAGGHDMDAVFCKRCGHEIH
                     PKVE"
     gene            complement(9946..10818)
                     /gene="prmA"
                     /locus_tag="AC159U_07455"
     CDS             complement(9946..10818)
                     /gene="prmA"
                     /locus_tag="AC159U_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3336933.1"
                     /translation="MFNLTKLSFEFSKTHSDMMSDFLSGVGAIGVAEDFIDDSKSEIN
                     SYYPNFTNMNEVIYKIKNYVAVLEGEYGKLYLGEFNVEEIDESKWQVWRDILKTVRVT
                     DRIIIRPPWEEHDTQNDEIVIEINPSMAFGTGHHESTRLCLEAAEKIITNEKIESVLD
                     VGCGSGILSIAAYKLGVNEVLAFDVDPVSIDETHQNMERNRISGKFTTKCCEIKDIQG
                     KFDLILANVYAEPLISMSDEIKSRLNNNGRAILSGFQDFRVKEIKLAYEKSGFTLVDT
                     IQDDEWVCAVFRID"
     gene            complement(10878..11438)
                     /locus_tag="AC159U_07460"
     CDS             complement(10878..11438)
                     /locus_tag="AC159U_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336934.1"
                     /translation="MDNLDNSIKQYYESQSLSEDRLERILGESSNKTSLKKLYIPLSI
                     AASLLIAVLASSVFYFDGYLLTKRVIKEIEMNHNKHLSVEMFSTEYAELSGMLKKLDF
                     ELVRPDSPFTKDYELIGGRYCSIQGELAAQLKLKHKGNGSISTLYAASLNPELQKIKL
                     TDKNTNTTSIKLWNSDNIFYGLAEDL"
     gene            complement(11439..11924)
                     /locus_tag="AC159U_07465"
     CDS             complement(11439..11924)
                     /locus_tag="AC159U_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3336935.1"
                     /translation="MDYRELVQYGYRYALSLTNNGHDAEDLVQEAFVKIKGKSKDVSK
                     TYFYKTIKSLFIDKYRRESLVVFDTEQAEQDSQLQETFDEEISVSAEHIEICLEVLRP
                     VEREVLFLSAVEQYTAEEIGNMMEKPRGTILSLIHRSKIKFRDKLRSLEHKDKISRDI
                     K"
     gene            complement(11958..12350)
                     /locus_tag="AC159U_07470"
     CDS             complement(11958..12350)
                     /locus_tag="AC159U_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336936.1"
                     /translation="MNKLVISAFSLMFVTLSMVVYAGDDPSIQGKPRTGSQTAMTNHI
                     NANTFDGKYVIYDAVQGKLLKLDFKELHAGLVKKGDFYVSCADFTDTQGNKYDLDFLV
                     GEKSNDYFVVQALVHKVNTDKRKYHLEN"
     gene            12521..12949
                     /locus_tag="AC159U_07475"
     CDS             12521..12949
                     /locus_tag="AC159U_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3336937.1"
                     /translation="MHTGQRIKAIRSKHSLTQHEFSNRIGISPNYLSELETGKAKPSK
                     PVLLALEHKYGVNYEWLVNGKGPESVELENKLNNEEREILSILRNNRELYQTVKNLSN
                     KYIKNKRILSNLSDKINISKEQALLLLTTVDKIKSRSKAF"
     gene            complement(12973..13392)
                     /locus_tag="AC159U_07480"
     CDS             complement(12973..13392)
                     /locus_tag="AC159U_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3336938.1"
                     /translation="MPFLITIRNVFLVTFLILFAANESFAKCEDPASPNVNWQGCNKV
                     GVYLKNKDLSGANLQGANLRGANFEGANLSGANLNEAILLGAKLEGANLEGATITSAF
                     LTKALVAGIKLKGAILDNTYWVNGKQCKPGSVGECIF"
     gene            complement(13404..14117)
                     /locus_tag="AC159U_07485"
     CDS             complement(13404..14117)
                     /locus_tag="AC159U_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3336939.1"
                     /translation="MSNSHLFLGEYEAILIDAQFNAEDAHNVVNLILQSGRKLSKIII
                     THPHPDHYYGLEVIGAKFSDAEIVGGHQTIEHIKNSAKYWGDNPQVFQNPKRYKTLSG
                     ISIKLDQTDLIYKIYKDSESVENTVLYIPSLKILFSGDLASNKVHMWLAEGNSKNWLN
                     HLIDIRSIGPVEKIYPGHGSVSDLRLISDAENYIKDFNRIAGSSKNKDEAAKRFMGIY
                     KNYQMPEILEASLMSVLNK"
     gene            complement(14293..14838)
                     /locus_tag="AC159U_07490"
     CDS             complement(14293..14838)
                     /locus_tag="AC159U_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002541338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3336940.1"
                     /translation="MRVFIITSVAAFIFVSFNSQNLIGDTYSSIVDDKGNITRPTDFK
                     EKWVYLGSWIHPDSLKDGFHNVYTQPGVVEKYKKSGGEFPDGAVLVKELRSSSTADMT
                     TGKNVHSADKEVLWFVMVKDKKGRFADNPKWGDGWGWALYYADNPSKDVSTDYKKDCL
                     GCHIPAKGTDWIYVNGYPVLK"
     gene            complement(14877..15284)
                     /locus_tag="AC159U_07495"
                     /pseudo
     CDS             complement(14877..15284)
                     /locus_tag="AC159U_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002541337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
BASE COUNT         5068 a         2881 c         2800 g         4661 t
ORIGIN      
        1 gatgtagcaa tgactggaga aattaccctt aggggtaagg tacttcccat cggagggctc
       61 aaagaaaaga ttcttgctgc tcaccgtgca agagtaaccc aggtgattat tccaaaggac
      121 aatctcaagg atacaaagga tattccatcc aatattctta aggatattga aataaagctt
      181 gttgagcata tggacgaagt gctcaaagaa gctataatat ctgatacaga gattctaaat
      241 gacaaaatag acagtacaca aattgatata acaaattcag agactcatag aattacccat
      301 tcttaaatag gtaataggag ccctgctgtt tggcaaggct cctattattt ccatttcaaa
      361 agcaatccag tagtattaat ctaaaatcac aagattgatt cgggagaaaa tatgcaggta
      421 aaacacatcc ctatagataa gcaaaaaaga attgcactgg tagctcacga caataaaaag
      481 gaaatccttc ttgactgggc aaaaaagaat aaagatgtac ttataaaaca taatcttttt
      541 gccacaggaa ctaccggcaa gttgatacag gatacccttg gagtaaaagt aaaaagattt
      601 aatagcggcc cgcttggggg tgaccagcag gtgggagcaa aaattgcaga agggaaactc
      661 gatttgctta tattcttttg ggaccctctg tctccccaac ctcacgaccc ggatgtaaaa
      721 gcgcttctca ggattgctgt gctatggaac atccccactg catgcaacag atctacagcc
      781 gattttatga tttcatcaag tctgataaat aaagcatatg acagaattgt gatagattat
      841 tctaagtata aacagagatt taataatcaa tccattaatt aaacgatgaa agaacaatta
      901 gaagaaacca taaatactgc aaataatgga aattgggaaa aggctcacaa aatagtccag
      961 gatatagagc atgagcttgc ttactggatt catgcaaatt tgcataggga agagggtgat
     1021 gtttctaatt ccaaatattg gtacaaaaga gcaggtaggg aatttactga gatggatttg
     1081 gaagaagaaa gaaggcttat taaagaagag atagaaaaca agctctgata aaattcaaac
     1141 aatcattgta gaatgttcat ccaataaact tcttatctcc aatccgtcat tttcaggaaa
     1201 ttggcttacg ggtatcacct gaaaatatga atcatttctg tatgccagga tcaatccatc
     1261 cctctccttc cactttacaa atgtgtccca tctaatcttt gtttctgact ttggggtttt
     1321 aacatacagg ttttctttat cgaagctcat atccacattt tgtatgtcat ctcccatctg
     1381 attgtactgt ttatttagct tccatctgat aattgaatta ttaagattta ttaagagtat
     1441 ggctgtaatt cctataaaaa cagatatata gccctgataa ctattggaat aatttttact
     1501 aataatccat atgccaaatg caattatggt aacagctaaa gaatatgcaa aatattttat
     1561 aaccttcctt ttccttcccc aatggatatt aaaagctcta accattattt ctattcctac
     1621 atctaccttc atgatttcaa cctctgtatt tttatttata aataaaaata tattatatat
     1681 aattaagcat taatatatta atattctaat caggggtaaa tataatcatg agatggagac
     1741 ttggcaggag aagcagtaat gtagaggaca gaaggggcag aagggttggc aggaaatccg
     1801 ctggcggtgg cattggtttt ataataatcc tgttaattgg gatgtatttg ggtatcgatc
     1861 caagcaccct tatgaacatc aaatccggaa ttgattcagt ttcagttcaa gagcaaaata
     1921 ctcaaacaac taaccccggg gtaaaggatg agctttcaga ttttgtttca gtagtgctgg
     1981 cagatactga agacacctgg aaccctttat tcaaacagat gggcggaaat tatagagaac
     2041 ccagactggt tctattttca ggggcagtga agtctgcatg cggacttgca cagtttgcaa
     2101 tgggaccttt ttactgcccg cgcgaccagc aggtatatat agatctaagt ttttatcagg
     2161 atcttaaaca taaattgggt gctcccggtg actttgcaca ggcctatgta atagcgcatg
     2221 aagttggcca tcatgtgcaa aaccttcttg gaatatctga gcaggtctac aatgcaagaa
     2281 caaggatgag taaggtagaa ggaaacaagc tgtcggtaat gcaggagctt caggccgact
     2341 gcttttcagg catctgggcg catcatgcag ataaggcaag acaaattttg gaaaagggcg
     2401 atatagatga agcccttaat gccgcaagca gcattggtga tgacagactg caaaaacaat
     2461 caacaggtca tgtaacccct gattctttca cccacggctc atctgctcag agggtaagat
     2521 ggtttaagat tggtctaaag agcggagaca taaattcatg taacactttt gaggctgaca
     2581 ggttgtaaaa taattgtgtt atttgccaaa tttctttaat ttatcaaatt cttccaaagc
     2641 aatatcttct tttgtctttt gaaattttag ccaactagtt tctattggtt tatttttgtt
     2701 ctttatatat ctcctcacta tataattatg attgtaatgc agattaacta gctcgagttt
     2761 tactttaaga ttagaagcac cacgaatatg cattggcttc tcaatataat caccatagtt
     2821 taagactaag ggaaatccaa aatactttgc tgaactatca aaatctgttt tacaacttcc
     2881 ttttctaata tattcattgg gtagtaacac atggtcatta tcaacaactc ttccatttac
     2941 cgttgcaaaa acattaaaac aataaataga aagatcgctt ggatgatata cagcagaaac
     3001 tattaacttc tgatcttcta aaccatcaaa tattcttatt aaacttattg catctaattt
     3061 taagcttatt gtatcttcaa attcatctaa tatctcgggg atgtcagcat gtaaatcatc
     3121 atttgctact ttaacattaa acctgataag gtcatgtgga cgccggcctt tatagatata
     3181 ttttgtagaa gaatttatag atccaaattc agtatttatt atttcctgtt tcttagttct
     3241 atttaataaa actttatctc ttgtaacttt aaacttattt aatataggat tagcgccaaa
     3301 gatattcttg gttccgtaat gttcaatgta gtgattatgt ttctcttcat atagtttata
     3361 tacattattt ttattttgta attcatcatt atcaatctca ccaacaggtc ttagatgtaa
     3421 agtattcgta aatattttta catctttaaa aaacttagta ttatgatcat gaagcaagga
     3481 tgacaaaaac acaaatacag acaaatacac tattgtatag atgacgtatc aatgaaattt
     3541 gtttttctta aaatagtcat cgaacctgga tagccattgt gatataatat tcatgattat
     3601 attctgcccc cacgtttatt ataggctcaa ttttcggtta atgcaattat tcaacatacc
     3661 aatcatctgc gtttacacga atagccaata cttcacctgt ggcagttttg agatcgtctg
     3721 aaaacatatc acaactaagc cagattttcc tgccttctct tttttgtata tatgcttcta
     3781 gttttacggg tttatctatt ggggtaggtt ttagataatc gacttttaaa gacgctgtta
     3841 cacaccatat taaaggtttt gagtcatatt ccctttcttc aatattatac tcatatgcta
     3901 ttgcagagca cacagtatga cagtctataa tagatgaaat tattcctcca tataaaaacc
     3961 caggtgggcc tgccgcatga tattcttcgg gcttccaaat acagacagat ttatcaccat
     4021 cccagtggct tttaatctta tggccatgtt cattgtgccc gcacccatag catttgttat
     4081 gtttccagta gtcctgaaaa ggtttatccg acattaatca tctccatttt tataaattta
     4141 tccaaatagc acatcagtat tagagcaatt atatcaaaca ttgtgttaag tcaccagatc
     4201 aagagacaat acctaacaac actaaaaccc ttgactaata taattattgg gtttattatt
     4261 tcagtagacg agcgagcgct cgctcgcatt taatgagaca ggacacatgg agcttttatc
     4321 aattagatat gggctcgggg ttaatagaca aaaagataat agataaaaac tgttggagat
     4381 aatttatgct ggaaacaaac cttactgatg atcagaaaat gatacgggaa ttaatctcag
     4441 attattcaga aaaggagatc aaacctgagg catcagaaaa tgagagaaat tccaggttcc
     4501 catctgagct tatagccaaa ctcggcgagc ttggatttat gggccatttt gttcctactg
     4561 agtatggagg cagtggcctt gattatctat cttatatcat tgcagtagaa gagatatcaa
     4621 aggcctgcgc atctacggga ataattgttt cagcacataa ttcacttgcc tgcagtccga
     4681 tagtttattt tggaacagaa caacaaaaac aaaaatatct aactgatctt gcatccggaa
     4741 ctaagctggg ctgttttgca cttagcgaac cagaagcagg ttccgatgct acaagtataa
     4801 aaactactgc agttaaaaaa gagaaacatt atataatcaa tggcttaaaa tcctggataa
     4861 caaacggctc agaggcagat gtggcaatag tgtttgcatc gacagataaa gataaaggca
     4921 gcagaggaat ttctgcattt attgttgatc tggattccga aggaattgag ataggcaaat
     4981 ccgaacacaa acttggaatc aaggcttcca gtactacaca gataatattt gatgactgta
     5041 tagtaccagc agaaaacctg cttggggaag aaggtgaagg gtttaagata gcaatgaaaa
     5101 ctcttgatgg tggcagaata ggcgtagcag cacaggctgt aggcattgca agggccgccc
     5161 ttgaagcatc aattatttac tctaagcaga gaaaagcatt taacgacact atatctaact
     5221 ttcagggcat acagtttatg cttgcagata tgtctacaaa agtagaagcc gcaagacttt
     5281 taacgtggca ggcggcgtct cttttggatc agggtgagaa atttacaaaa cagtcagcta
     5341 tggcaaaact atttgcagca gagaccgcta tgtgggtcac tacaaaagca atccagatac
     5401 acgggggtaa tggatatacg actgactttc ctgttgaacg gtactttaga gacgctaagg
     5461 ttacagagat ttatgaaggc acctcggaga tacaaaaaat agttatagca agacaggcac
     5521 ttaatgaata ttgatttatt acagattaca aaatgcaaaa tgaagttcaa tcaaagataa
     5581 aaaataaaaa gctgattgat acaaggcgaa cagaacttat aaatgcagct gtaaagctct
     5641 ttgttattaa aggctttcat gaaacaagtg taagggaaat tgctaaagaa tccggcatga
     5701 gcatgggtgc gctttatgac tatataagaa caaagaatga tattcttttt ttggtttgtg
     5761 atcacattca ctcaagcatt agcactaagc tcaaagaatc cgttgcaagc aataagcctc
     5821 caattgatat tctcaaaaat gctatcaaag actattttaa aataatagat aaaaatcaaa
     5881 acaatatgct ccttttatat caggaaacca aatctcttag caaaagtgcg agaaaatata
     5941 tttttaaagc agaaagcgat ttaacatcta tttttaaaga tatattaata caatgtataa
     6001 aagataaatc tgtttcaatt agcaaaaaga aagctgatat agtctctcac aatatcatgg
     6061 tagcaggaca gatgtgggca ttcaggcgct gggctctaag tgaaaattat acaataaatt
     6121 catacataaa ttcccaaaca gagttattac taaatggagt aaaataatta tgggcaaaat
     6181 aataaatatg gtaggcattg ttggatcagg aaccatgggc tcgggtattg cgcagcttgt
     6241 ggctgcttca ggttacgaga tagtcctaat tgatttaaac gatgaactgc ttaaaaaatc
     6301 ggtatccagt attaataaga acctctctag acttgtggac aaagaaaaga tcacaaaaga
     6361 gatccaccag caaattctaa aaagagttaa tacatctaca aaccttgata atctttctag
     6421 ctgtgatctg gtcatagaag ctgtatctga gaatatcgac ataaaaagag aactatttgt
     6481 aaagttaaat gcggtcgtaa aaaaagactg catcatcgca tccaacacct ccaccctgtc
     6541 tataaccgag cttgccatgt ttacttccag gccagataaa gtggtaggca tacatttttt
     6601 caatcctgct ccagttatga aactggtcga aataattaaa tcattggcca caagcaaaca
     6661 gaccattaaa acagctgaag agtttatact cagcctcgat aaggaccccg ttgttacaaa
     6721 agacagaccc gggtttatag tgaaccggat tttagttcca atgttaaacg aggcagtatt
     6781 cgcactagat gaacaaatgg gaactgctga ggatatagac aaagccatga agcttggcac
     6841 taatcaccct atgggacccc ttgagctaat agatttaatc ggactggatg tgacacttga
     6901 tataatggat gttttatttg ctgaatttaa agatagtaaa taccgagcct gcccactact
     6961 aagacaaaag gtaagagctg gtcatctggg aagaaaatcg ggaagaggat tttatgacta
     7021 ttaatttaag gagagaataa gatgtcagaa aatattattt ataaaaagat caagcctaac
     7081 attggtcttt taacgatcaa tagagaaaaa gctctgaaca cactcagcct tgaaaccgtt
     7141 accgagctaa aagaattcat agacaatcaa cttccaaaag aagaaataag agtattaata
     7201 atcactggtt ctggcaaaaa agcatttgtg gccggggccg atatttcaca gatgaaagag
     7261 atgaacaagg atgaatttaa aaactactgc gaaatatctc acggcaactt taataatctg
     7321 caaaaattaa taattccggt tatagccgcc ataaacggat atgcgcttgg aggtggtagc
     7381 gaacttgcgc ttgcctgtga tatcagaata gcttcggata atgctaaatt aggctttccg
     7441 gaaacaaagc ttggattgtt tccttgctgg ggaggttctc aaagagcgct tagactactt
     7501 ggaattgcaa aggcaaaaga gctaattttc actggggaga tgatatccgc agaacaggca
     7561 gcagaaatcg gcatggtaga taaggtagta aaacaggatg atctcatgga cgaagtggtt
     7621 tctattgcag agaaaatatc tggtaacagc ccacttgcag ttggatacgc taaagaaatg
     7681 ataaatagcg gtcaggattt ggtgttatct gaatcacttg agctggagct tcaaaaaggg
     7741 attgagtgtt ttgattcata tgacaggcaa gaaggtatga gtgcatttat agaaaaaaga
     7801 actgctgaat ttaaggggac ctaaaatgga aaaagtcgta ctttgtaatc cactgagaac
     7861 tgcaattggt acatttggag gaacgttaaa ggatacaagt gcagttgatc tgggaataac
     7921 cgttgttaaa gaaatattaa atcaatccag tctggaccct aaaaatattg atgactgtat
     7981 catgggaaac atcctgggag caggccttgg tcaaaatcca tccagacaaa ttgctatcaa
     8041 ttcaggactc cccgtagaaa ctcccgcttt aactttaaat aggctctgcg gttccggtct
     8101 tcagtctgtt atatttgcag ctcaggcaat aaaggctggg gatgcggact gtatcgtggc
     8161 tggcggcatt gaaaatatga gccaggctcc tttttatctt aagaaggcac gctggggcta
     8221 taaaatggca atgccaaaag aagaaattat tgacggtatg gtctacgatg gactatggga
     8281 tatattcggt gattatcata tgggtataac cgccgaaaac cttgccgagc agtacaatat
     8341 atcaagagag aggcaggatg aatttgctta caatagccaa atgaaaacta aacaggcaca
     8401 ggaagatggg aagtttgatt ctcagattgt gcctatttct attcccaaaa gaagtggtga
     8461 atatactaaa tttaaaacag acgaacaccc aaggggtgac gttacaatcg aatcactgaa
     8521 taaattaaaa cccgtattca aaaaagacgg cacagtaaca gccggaaatg cttcaggcat
     8581 aaatgacagt gcagccgcaa taatagtttg ttcagaatca aaggcatctg agctggattt
     8641 agaacctctt gcatttataa ggtcatatac acttgctgga gtggatcctt ctataatggg
     8701 tatcggaccc gtgcccgcaa ttcaaaaagc cctaacaaaa gcaaacctat caatagatga
     8761 tatagactta tttgaattaa atgaagcttt cgcagcacag tccttagcat gcctaagtga
     8821 tcttccgatt cctgagaata agctcaatgt aaacggcggc gctattgctc ttggtcatcc
     8881 cattggagca tcaggggcag taatactggt taaattactt catgaactaa aacaaagaaa
     8941 agaggctaaa cttggcctct gttcactttg tataggcggc ggaatgggca ttgctatggt
     9001 agttgaaaaa attaagtaac tctatattac atatatctgt atcactcccg attattattc
     9061 gactttcgga tgtatttcat ggccgcaacg tttgcaaaaa acggcgtcca tatcgtgtcc
     9121 gcctgcatta cagtgcagac atacctgagt agacaaattt cctttaaatg catttgcaat
     9181 ttccacagtt acaataccgg tgggaatagc aataatactg tagccgataa tcatgattat
     9241 ggatgcaatg aatttgccaa ggttggtaac cggagctaca tctccataac cgactgtagt
     9301 gattgtcaca attgcccagt aaatgctctt aggaatgctc gaaaatccgt ttttttcgcc
     9361 ctccacaaga tacataatag agccaagaat gattacaagt gttagtactg tcatgagaaa
     9421 aacaaatatc tttcttctgc tggctacaag cgcctgcaaa agaagggtgg actcacctac
     9481 ataatgggca agtttcagca ccctgaatat ccttaatatt ctaagcaaac gaaccaccag
     9541 gaaatactgc gagcccggaa gaagaagact cagataagtt gggactatcg ccaaaagatc
     9601 tacgattcca aaaaagctta tggcatattt aatcctgttt cctacacagt atagacgaag
     9661 cacatattca attgtaaaaa ctatggtaaa aaaccattct gccagataga gcaggttgcc
     9721 atattgctca cggaatgatc ttacgctgtc gagcatgaca aaaataacgc taagaacgat
     9781 acaggagatt aggacaatat cgaaaatctt gccaaggaag gtatcggctt cgaatattat
     9841 ctcatgaagc ttaaaccttg tggttgaaat tgaattgttt tccggcttgg tgtccattac
     9901 ataatattaa tgaatatttg aagttattaa aaatgaaatg ataatttaat ctatacgaaa
     9961 cacagcacaa acccattcat catcctgtat tgtatcaaca agtgtaaaac ccgacttttc
    10021 ataggctaat tttatttctt taaccctaaa gtcctggaaa ccggataata tagctcttcc
    10081 attattattt aacctggatt ttatttcgtc actcatgcta atgagaggct ctgcataaac
    10141 atttgcaagg atcaaatcaa acttgccctg tatatctttt atctcacagc acttggttgt
    10201 gaattttcct gaaatccggt ttctttccat attctggtgt gtctcatcaa ttgaaacagg
    10261 gtcgacatcg aaagccaaaa cctcatttac gcccagctta taagctgcaa ttgataaaat
    10321 tccgcttccg cagcctacat ccagtacgct ctctatcttt tcatttgtaa tgattttttc
    10381 tgctgcttca aggcatagcc ttgttgattc atgatgccct gttccaaatg ccatggatgg
    10441 gtttatttct ataacaattt catcattttg tgtatcgtgc tcctcccatg gtggtcttat
    10501 gataattcgg tcagtaaccc ttacagtttt tagaatatcc ctccagacct gccatttgga
    10561 ttcatcaatt tcttctacat taaactcccc aagatacagc ttgccatatt caccttcaag
    10621 aacagccaca tagtttttta tcttataaat aacctcgttc atatttgtga agtttggata
    10681 gtaagaattt atctcagatt ttgaatcatc aataaaatcc tcagctacac caatggctcc
    10741 gacaccggag agaaaatcgg acatcatatc ggaatgtgtt ttgctaaact caaaggaaag
    10801 ctttgtaaga ttaaacatag aggagatatt taatataaaa gactctatag tgcaaataat
    10861 tttgtctttt atttgtatta aagatcttca gcaagcccgt agaaaatatt atcgctattc
    10921 cacagtttta tagaggttgt attcgtattc ttatcggtaa gctttatttt ttgtaactcc
    10981 ggatttagac tcgccgcata aagggtagag attgaaccgt tacccttatg ttttagtttt
    11041 agctgagctg caagctcacc ctgaatggag cagtaccttc cgccgataag ctcatagtct
    11101 tttgtaaatg gagaatcggg tcttacaagt tcaaaatcaa gctttttaag catgccggaa
    11161 agctcggcat attctgtgga aaacatctcg acagaaagat gcttattatg attcatctca
    11221 atttctttta ttaccctttt agttaaaaga tagccatcaa aataaaatac cgacgaagca
    11281 agaacggcta tcagcaatga tgccgcgata gataaaggaa tgtaaagttt ctttaaactt
    11341 gtcttgttgg aagactcccc aagaattcgt tccaggcggt cctcagacag cgactgagac
    11401 tcataatact gctttatact attatctagg ttatccattt atttaatgtc cctggatatt
    11461 ttatccttat gctccaagct tcttaattta tccctgaact taattttaga cctgtgaatc
    11521 aaagagagaa tagtaccccg tggtttttcc atcatattac ctatctcttc tgctgtatac
    11581 tgctcaactg cgcttaaaaa taaaacctct ctttcaaccg gcctcaatac ctcaagacat
    11641 atttcgatat gttctgctga tactgatatt tcttcatcaa atgtttcttg taattgagag
    11701 tcctgctctg cctgttctgt atcaaacaca acaagactct cccttctgta tttatctata
    11761 aaaagagact tgattgtttt ataaaaatag gtctttgaaa catctttgct tttaccttta
    11821 atttttacaa atgcttcctg caccaggtcc tctgcgtcat gaccattgtt tgttaaacta
    11881 agtgcatacc tatatccata ctgcacaagc tctctgtaat ccatattaga tcagtataac
    11941 cttcaaaggc tattaaatca gttctccagg tgatatttgc gtttgtcagt atttacttta
    12001 tgaacgagcg cctgaactac aaaataatca ttactttttt cgccaaccag aaaatcaagg
    12061 tcgtacttat tgccctgagt atctgtaaaa tcagcacagc ttacataaaa atcacctttt
    12121 tttacaagcc ctgcatggag ctctttgaag tcgagtttaa gtaattttcc ttgcactgca
    12181 tcataaatca catatttgcc atcaaacgtg ttagcattta tgtggtttgt cattgctgtc
    12241 tgagaaccag ttctgggttt accctgaatt gagggatcgt cccctgcata aacaaccata
    12301 cttaatgtta caaacataag agaaaatgct gatattacta acttgttcat ttactgcctc
    12361 ctgaattcat caattttata tatataaacg aatatatagc tgttttgatt gcaattattt
    12421 tgtatagatg ctgaatttaa taattttttg gaatattctg aaactttttc tattaatttc
    12481 tcatcacata tataaagaat agcattataa agaggttact atgcatacgg gacaaaggat
    12541 taaagcaata agatcaaagc actcgcttac tcagcatgaa ttttccaata ggattggtat
    12601 ttcaccaaat tatctgtcag aactggaaac cggtaaggca aagccttcaa aacccgtgtt
    12661 attagccctt gagcataagt atggagtaaa ctatgaatgg cttgtaaatg gaaaaggccc
    12721 ggagagtgtc gagcttgaaa acaaactaaa taacgaagaa agagagatat taagtatatt
    12781 aagaaataat agagagctat atcaaacggt aaaaaatcta tccaataaat atatcaagaa
    12841 taaaagaatc cttagcaacc tttcagataa aataaatatc tctaaagagc aggcgcttct
    12901 gcttttaact acagtggaca agatcaagtc caggagtaag gcgttttgaa tattctattt
    12961 attaaagatt aatcaaaata tacattctcc cactgaaccc ggtttacact gcttaccatt
    13021 aacccagtaa gtattatcca atattgcacc ctttaattta atacctgcaa caagtgcttt
    13081 tgtaagaaat gctgaagtaa ttgttgctcc ttccaggtta gccccctcaa gcttagcccc
    13141 aagcagaata gcttcattca aattagctcc ggaaagattt gctccttcga aatttgcacc
    13201 tcttaaattt gcaccctgca ggtttgctcc acttaagtct ttattcttta gatagactcc
    13261 tactttattg catccctgcc agttaacatt cggacttgca ggatcttcac actttgcaaa
    13321 tgattcgttt gctgcaaaca agattagaaa tgtaactaaa aaaacatttc gaatagtaat
    13381 taaaaaaggc ataaaagtat ctcctatttg ttgagcacac tcattaaact tgcttcaaga
    13441 atttcgggca tttgataatt tttataaatt cccataaaac gtttagcagc ttcatccttg
    13501 ttttttgaac tacctgcaat tctattaaaa tcctttatat agttctcggc atcggaaata
    13561 agtctcaaat ctgatacaga accatgccct ggatatattt tctcaaccgg tcctatggac
    13621 cgaatatcaa ttaaatgatt aagccagttt tttgaattgc cttctgcaag ccacatatgc
    13681 actttattgc tcgctaagtc gccactaaat aatatcttaa gagacgggat ataaagaaca
    13741 gtattttcaa cactctcact atctttataa atcttgtaga ttaaatcagt ttggtctagt
    13801 ttaatactta tcccacttaa tgttttatac cttttggggt tttgaaacac ttgtggatta
    13861 tcgccccagt acttagcgct gtttttgata tgttcaatag tttgatgccc gccaactatt
    13921 tcagcatcac taaatttagc tccgattact tccagaccat aataatgatc tggatgcggg
    13981 tgtgtaataa taatcttaga cagtttcctg cctgattgga gaattaggtt taccacatta
    14041 tgagcatctt ctgcattaaa ctgtgcgtcg attaaaattg cttcatattc gcccaggaat
    14101 aaatgcgaat tactcataaa tcccttggcg gaggcagatt aggtgagtac agatagtttg
    14161 tttttaggct ctggagacct atttgattgt agattggtgc aggaaaatag aactatagaa
    14221 agcgctataa tacagcagct agaacaattt aaaattctga gtagtatctt aattgttaat
    14281 ttattaatat atttatttta atacaggata tccatttaca taaatccagt ctgtaccttt
    14341 agcaggtatg tgacatccta aacaatcctt cttataatcc gtagagacgt cttttgatgg
    14401 gttatcggca taatacaaag cccatcccca gccatctccc cactttggat tatcagcaaa
    14461 ccttcctttt ttgtccttaa ccatcacaaa ccaaagcact tctttatcgg ctgaatgtac
    14521 attcttacct gtcgtcatat cagcagtact agatgatctc agttccttaa ccaatacagc
    14581 gccgtctgga aattctcctc cactcttttt atacttttca acaaccccag gctgagtata
    14641 aacgttgtga aaaccgtcct tcaaactatc cgggtgtatc caggaaccca gatacaccca
    14701 tttttcttta aaatcggttg gacgagtgat attccctttg tcatcaacaa tggaagaata
    14761 tgtatctcca attaaatttt ggctattaaa tgacacgaaa atgaacgcag ctacgctagt
    14821 tataataaaa actctcatta tatttacctc tataaacttg accagctatc tttactctat
    14881 ttctttttca aagcacgcag attagtatag tactgtgtga acacccagtc atcagctgca
    14941 gctgcctgat gacaggcatt acatgcagca gcgggaaata tttttgccgt atctttgtaa
    15001 ggaagagaat gatccggttt ggaaaaacta aaataccccc aattgcctgg ttctttaggg
    15061 aatctttttg aatctttaat agttgcttcc agtcctaaaa attcaccttc aaaataacca
    15121 ttaccgctca cagcactctt ggaaccgacg cttataagtt ctttcataag tatagttcct
    15181 tctctgaact tacccgtttc tttataatgt ttataactta caggatctat atatacgtga
    15241 tggaactcag ggaaaggggc tttgccgttg ttcatgtcat taggggcata attgcaaaaa
    15301 atgtgtgctg aaaatggggt tttagccttt atttactata ttataatgag aaaaaagtta
    15361 aacaggttta actatgaaca aaaaactgtc ccctttgcag tgcacaaaaa
//
LOCUS       DBNGSV010000204         5740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_35522, whole genome shotgun sequence.
ACCESSION   DBNGSV010000204 DBNGSV010000000
VERSION     DBNGSV010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_35522"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..2321
                     /locus_tag="AC159U_07500"
     CDS             132..2321
                     /locus_tag="AC159U_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013636.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HIR3336941.1"
                     /translation="MDYIFQNRTSRLLISLIILLLTANFSIAAIFTYDNATPGTLNEV
                     AASCGSPLIRNFSVTDNFTINDLNVGFNANHTFRGDIIVDLESPMGTTVRIISSNGGD
                     GNNNYDLLLDSASGNPINDGNGDNTAVPIYDGDRTAAPSSTLNVFNGQNANGIWEMRI
                     CDTFAGNGGTFNSARLVFDGTSVKNPGEVTGTAFRDYNANGSQDTNEPGISGIEVFAT
                     DENGTTASTTTGSDGSYIIAAGAGLTGNVRIEFSLPAGGALDFLEDGAPGATNVQFFD
                     ITSGACANAAFNNPAQFCESNPTMVTNCYVDGDNLIGPNQDQDVLVSFPFNSSGDITP
                     PNHEAEAEQIGTTWGLAYQRSSDTLFAGAFQKRHTGYGPNGTCAIYELENPGDNQDGD
                     GDAGIAPALFVDLNSTFSVNCGTNPHPGGTNFSVDSASFDAVGKRALGDMDISEDDLT
                     LWTVNLNTKDLIEIPLGTDPTTPTVPGAIFTHPTTALDDVCAGAPVNASASDFRPFAL
                     EVHDGRVLLGGVCSAESNGLVSSLYGVVFEFNPGNTSGTGSFNTSPLLLFPLNYDRGC
                     GFGIASITTCFGPADWEPWVANWGGFSWNVLFGDGTREIGLPQPMLTDLELTEEGDLI
                     IGLRDRFGDQTGFAELATNPPGGGTIFSGDGEGDILLATSNGSGWNQPSVPADGTSDT
                     AFPPTSNEFSLQATFSSTLSFSTKKHQWPVLRLYLGQQPRLTLLWIL"
     gene            2258..5305
                     /locus_tag="AC159U_07505"
     CDS             2258..5305
                     /locus_tag="AC159U_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR3336942.1"
                     /translation="MAGLALVLGTATPAYTVMDPVNTAFSSGIDWVQTTGSKADHTHR
                     SYEIIAGAPGTGKAGGLGDLEALCSPAPIVLGNRVWNDTDADGVQDPGETGLGSIIIQ
                     IYSDPDGIPNNGYEVLIGTTTTDGSGNYLITDAVLQNGPDGINGNGDDDPNAAVTLNT
                     NYLITIDPSQSGISTNNFIVTFPDTDEEQRDSDATRLLNNGGSLTDFIAIPLSTSGAG
                     VNDYNMDFGFTAQENNPVSLYSFEAYYVDNSLKFDWVTGTETHNAGFNLWVVINGKKH
                     YLNDDIIPSHLTDSLIPQYYQQLIPAGDIKDEFMLLGLSSIDIKGKEEFHGPFKLGEK
                     YGSAPVDQQIDWRAIRSGIDKQLESKNLVLRDNSLRAVNKGKFTTTLANSSSAGGGNN
                     SSPDSVCNVTVGSRGINRVTFTQLQSAGCDYTGINPTDIAVIYKGAGISRYIESGSAN
                     GKKFSTGGYIDFYGELPQDGDSLYLKENTYQIKIDRTLAVNSKLVNSKPSGSDSSYTE
                     TTLRHHNLIYSFTMASENDPWYERRIFSRGSPVSADFEVVTESDALTTDPAVLTVSLA
                     GGIDAPGVDPDHHVKIYINGNQVGDFFNNGFVDWLIEIPLAGNIILPGSNIVTVEAVG
                     DTGFPFDIVLLNYIELSYERPYTAVADKLLFEETVQSDFVVTGLSTDSVVSYAYDGID
                     LYILKAETAANAGVFDVSIPGVQNNSTSAVKYWVSAENKINNTLVSLARSNTDILSGF
                     ADYLIIAHPAFIGAELDGYVSQKESEGYAVKVVDLLDIYDDHGSGMATPFSIRNYLKE
                     AAPSIGFDHLLFVGGDSYDYHNNTSSGVISFIPTMYELTNELIFYTPTDSLIADLDGD
                     EIQDLSIGRWPVRSIDYLGAIINKTSTFSSTSTSSALLVAEHTDPSKEPFKNQTDRTA
                     NRLPFEPVDIVKVYVDDFLDADPDLTISGAIAQTRDTMISRINNGTSLTIFAGHGAPT
                     MWTFNGLMTPTIAQNLTNAGKPTFIMPLLCLLHATPHITLFLR"
     gene            5260..5553
                     /locus_tag="AC159U_07510"
     CDS             5260..5553
                     /locus_tag="AC159U_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR3336943.1"
                     /translation="MPLACYTTYHVIPTLNSLSHQLMAAGDKGAVVVTGAISLSTLAD
                     NEKLGDGIIDKMFYENKTLGQAVKETKQILGTEFRDVVINWQTLGDPTLKFNQ"
BASE COUNT         1737 a         1173 c         1266 g         1564 t
ORIGIN      
        1 gtttcgaata attattaacg gcataaatat tgcagtactc atctaatact ctttcaatat
       61 tcgagagtac acaatacagg tagaggggaa ataataattg ataaatctaa ttattattaa
      121 gagattaaac tgtggattat atttttcaaa atagaacgag caggcttctt atctcgctaa
      181 ttattctttt attaaccgct aatttttcta tagcagcaat ttttacttat gacaatgcaa
      241 cacccggcac tttaaatgaa gtagcagcat catgcggttc tccattaatc cgaaacttct
      301 cggttaccga taattttact attaatgatc tcaatgtggg tttcaatgct aatcacactt
      361 ttaggggcga tattattgta gatcttgagt cccctatggg aaccacggtt agaattatta
      421 gttctaatgg tggagacggt aacaacaact acgatctgct tttagatagt gcatcaggca
      481 accccataaa cgatggtaac ggtgacaata ccgctgtacc catttatgac ggtgacagaa
      541 ctgccgcacc aagcagcacc ctaaatgtat ttaacggtca aaatgctaac ggcatatggg
      601 aaatgcgtat atgtgatacg tttgcaggta acggtggtac ttttaacagc gccaggcttg
      661 tttttgatgg aacttcagta aagaaccccg gagaagttac aggaacagct ttcagggatt
      721 acaatgcaaa cggctctcag gatacaaacg agccgggaat atcgggcata gaagtatttg
      781 caacagacga aaacgggact actgcctcca caacaacagg cagtgacggc tcatacatca
      841 tagcagccgg tgcagggctt accggaaacg taagaattga gttttctctt ccagctggcg
      901 gcgcacttga ttttcttgaa gacggcgccc cgggggctac aaatgtgcaa ttttttgata
      961 taacaagcgg agcatgcgca aacgcagcat ttaataatcc agcacagttc tgcgagtcta
     1021 atcccacaat ggttacaaac tgttacgttg acggagacaa cctcataggg ccaaaccagg
     1081 atcaggatgt attggtatct ttcccattta attcatcagg agatataact cctcctaatc
     1141 acgaggcaga agccgagcag atcggtacta catggggact tgcttaccag cggtcatcag
     1201 acacactttt tgcaggagca ttccagaaaa gacacacggg atacggccct aacggcacat
     1261 gtgccatata tgagctggaa aaccccggag acaatcagga tggtgacgga gatgcgggta
     1321 tagcacctgc tctttttgta gacctaaact ccacattttc agtaaactgc ggtactaacc
     1381 ctcacccagg aggtacaaat ttttcagtag acagcgcatc ttttgatgct gtggggaaaa
     1441 gagctcttgg agatatggac atctcagagg acgaccttac actctggact gttaatctga
     1501 acacaaaaga tttaattgaa ataccccttg gtacagaccc tacgactccc acagtaccag
     1561 gagctatatt tactcatccg accacagcgc ttgatgatgt atgtgcaggc gctcctgtaa
     1621 atgcaagcgc ttctgacttc agaccttttg cgcttgaagt tcatgacgga agggtactcc
     1681 ttggaggagt atgcagcgca gagagcaacg gacttgtatc aagcctctac ggcgtagtat
     1741 ttgagtttaa cccgggaaac acaagcggca ctggatcatt taacacatct ccactactgc
     1801 tctttccact taattatgac agagggtgcg gctttggcat tgcttcaatt acaacctgct
     1861 ttggtcctgc agattgggaa ccatgggttg caaactgggg cggattctct tggaatgtat
     1921 tatttggcga tggaaccaga gagataggac ttccacaacc tatgttaaca gacttagaat
     1981 taacagaaga aggagatctt ataattggac ttagggaccg atttggtgac caaacgggtt
     2041 ttgctgagct tgcaactaat cctccgggag gaggtaccat tttttcgggt gatggtgaag
     2101 gtgatatact ccttgcaaca tccaatggaa gtggatggaa ccagccatct gtacctgcag
     2161 acggcacatc agatacggca tttccaccta caagcaatga attttctttg caggcgactt
     2221 tttcttcgac actgagtttc tccacgaaga aacatcaatg gccggtcttg cgcttgtact
     2281 tgggacagca accccggctt acactgttat ggatcctgtg aataccgcat tttcaagcgg
     2341 aatagactgg gtacaaacaa ccggatcaaa agcagatcat acgcacagaa gctatgagat
     2401 aatagcggga gccccgggaa ccggaaaagc cggaggtctt ggtgatctgg aagcgctatg
     2461 cagccctgct cctattgttc ttggcaacag ggtatggaat gatacggatg cagacggggt
     2521 gcaggatccg ggtgagacag gtcttggaag tataataatt cagatttact ccgatcccga
     2581 cggcatacct aacaatggtt atgaagtgtt gataggtact actacaaccg atggcagcgg
     2641 taattatctt ataacggatg cagttctgca aaacggcccg gacggtataa acggcaatgg
     2701 tgatgatgat ccaaatgccg ccgttacgct taacacaaat tatcttataa ccatcgatcc
     2761 ttcacagtca ggtattagca ctaataactt tattgttaca ttcccggaca ctgacgaaga
     2821 acaaagagat tcggatgcaa cccgacttct taataacgga ggatcactca cagacttcat
     2881 tgcaattcct cttagcacat caggtgcagg cgtaaatgac tataatatgg attttggctt
     2941 tacggcacaa gagaacaatc ccgtttcatt gtattcgttt gaggcatatt acgtagataa
     3001 ctcccttaag tttgactggg ttacgggtac tgaaacccat aatgcagggt ttaacctctg
     3061 ggttgttatt aatggtaaaa aacactacct aaacgacgat attatcccaa gccacctcac
     3121 agattctctt atcccccagt actatcagca gcttatccca gcaggtgata ttaaagatga
     3181 atttatgctc cttggcctca gcagtattga tataaagggc aaagaagaat tccacggccc
     3241 gtttaaactg ggtgagaaat acggaagcgc tccagtagat caacaaatcg actggagagc
     3301 cataagatcg ggtattgata aacaactcga atccaaaaac cttgtgctaa gggataacag
     3361 ccttagagca gttaacaaag gtaaatttac cactaccctt gcaaacagca gtagtgctgg
     3421 aggcggcaac aattcatctc cagactctgt atgcaatgta accgtaggct caagaggtat
     3481 taacagggta acatttaccc agcttcagtc tgcaggatgt gattataccg gaataaaccc
     3541 aacagatatt gcagttatat ataaaggcgc aggtatttcc agatacattg aatcaggaag
     3601 cgctaatggg aagaagttta gcacgggcgg ttatattgac ttctatggag agctccctca
     3661 agacggtgat tctctttatc ttaaagaaaa tacttatcaa ataaaaatag acagaactct
     3721 tgctgttaac agcaaattag ttaactctaa acctagtggt tcggatagtt cttatacaga
     3781 gactacactt agacaccata acctaatcta cagctttacc atggcctcag agaatgaccc
     3841 atggtatgaa agaagaatct tttcaagagg cagccccgta tctgctgatt ttgaggtagt
     3901 tacggagtct gatgccctta ctacagatcc tgccgtgctt acagtgagtc ttgcaggcgg
     3961 gatagatgct ccaggtgttg atcccgatca tcatgtaaaa atatatataa atggaaatca
     4021 agtaggtgat ttctttaata acggttttgt ggactggtta atagagatac cccttgcagg
     4081 gaacatcata ttaccgggtt caaatattgt aaccgtagag gctgttggag atacgggatt
     4141 tccgtttgat atagtgcttt taaactatat tgagcttagt tatgaaagac catatacagc
     4201 ggtagccgat aaactgcttt ttgaagagac agtacagtct gattttgtag ttacggggct
     4261 tagcacggac agcgttgttt cttatgctta tgacggaatt gatctttata tactaaaagc
     4321 tgaaactgct gcaaatgcag gagtgtttga tgtatcaata cccggggtac aaaacaatag
     4381 tacatctgct gttaagtact gggtatctgc agaaaacaag ataaacaaca cgcttgtatc
     4441 tcttgcaagg tcaaatactg atatactatc ggggtttgcc gattatctta ttattgcaca
     4501 tccggctttt ataggagcag agcttgatgg ttatgtatcg cagaaggagt ctgagggtta
     4561 tgcagtgaag gtagttgatc tgcttgatat atatgatgat cacggctctg gtatggctac
     4621 accgtttagc atacgcaact atctcaaaga ggctgctcct tcaataggtt ttgatcatct
     4681 gctttttgtg ggtggggata gttatgatta ccacaacaac acaagctctg gggttataag
     4741 ttttataccc acaatgtatg agcttacaaa tgagcttata ttctatactc ctacagactc
     4801 attaatcgct gatcttgatg gggatgagat acaggatctt tcgataggca ggtggcctgt
     4861 aaggagcatt gattaccttg gtgctattat taacaagacc tcgactttta gctctacctc
     4921 tacctcttca gcattattgg tagctgaaca taccgatcct tccaaagaac cttttaagaa
     4981 tcaaacagac aggacagcta ataggcttcc atttgaacca gttgatattg ttaaagtata
     5041 tgtggatgac tttttggatg ccgatccgga tcttactata agcggagcta tagcacagac
     5101 tagagatact atgatatcaa gaataaataa cggaacaagc cttacaatat ttgctggaca
     5161 tggagcgcct acaatgtgga catttaacgg gcttatgaca ccaactattg cgcagaatct
     5221 tacaaacgca gggaagccca catttattat gcctctatta tgcctcttgc atgctacacc
     5281 acatatcacg ttattcctac gctaaactct ttaagccatc agcttatggc agcaggagac
     5341 aagggagctg tggtagttac cggggctata tcactcagta ctcttgcaga taatgaaaaa
     5401 cttggggatg gcattataga caaaatgttt tatgaaaata aaaccctggg acaggctgta
     5461 aaagaaacta aacaaatctt gggtacagag tttagggatg ttgttataaa ctggcagacg
     5521 cttggtgatc ctactctaaa atttaatcaa taaaaatagt ctatgggtaa accggagtaa
     5581 tttcggttta ccttctttta ctttcaagat ataaacttag ggcattagac gttgttagga
     5641 gtagtatata tgataaagag caatgataaa gcggtattca tggcaattaa cagtgggaaa
     5701 aatatcacga attttttcac acgcaaaata tggaaaaaac
//
LOCUS       DBNGSV010000205         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_35663, whole genome shotgun sequence.
ACCESSION   DBNGSV010000205 DBNGSV010000000
VERSION     DBNGSV010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_35663"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1379
                     /locus_tag="AC159U_07515"
     CDS             33..1379
                     /locus_tag="AC159U_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3336944.1"
                     /translation="MKNLLILIVLSSISIALYQYLPVEKDTAKGLAVLFLIGGLWVSE
                     ALPITVTALAVPLIAVLTGIFDAKDSFKHFANPIIFLFLGGFALAAALHKQNMDELIA
                     KIVLKTSRNNVLVSAIILFLVTAVISMWISNTATMAMMLPVALGLLYRINKIEDNRIN
                     LFILLGLAYSANIGGIGTIVGSPPNGITAANLGMNFNDWLYVGIPSVLILLPVAVLFL
                     YLWIKPVFLYKETSDQMSINYSDYFNKNSILVVVIFFITVFLWLFSKPISGYLQITKS
                     FDSLVAIFALILLCATRVVNWKEIEEFTDWGVLLLFGGGIALSAVLSETGASKYLANI
                     LFSNLFDFGVLILLLGGVLLVVFLTEIASNTAIAAILVPIFYAVGVEIPTLNNSVMPL
                     AVGIAASCAFMLPVATPPNALVFGTGKVSHKSMIKIGLGLNILCAVLVCLITYLVI"
     gene            complement(1417..2490)
                     /locus_tag="AC159U_07520"
     CDS             complement(1417..2490)
                     /locus_tag="AC159U_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336945.1"
                     /translation="MIRLKTLIISTFLIFVFTIQSSANEQKIFSLLENSGVLIFGEKR
                     GKPESARLVSDVVEAFTAMGNCVNVGLEISSEEQKNIDGFINGNTDISNIRINTFVTH
                     KEYRNMLRSFKDFKAAGRCIRVFGVDVPDTVPIEKNVWMSRIINSLVGDSPMLVLIGN
                     RHTFKNVQWKNDENKKTVLAQNLKSNGVNVGAVMQYWEKQDCISKIADFLDTENPYTG
                     VYVNDIIKPLSMKPPSQVSEITDGVIVWNCGTLSAEINKQVEIEKKTADIDVDPVINK
                     PTIETDRKKIEKSIKRGIPMAGMTTEDVIKTLGEPKKKSGVNSSGYEKWGYECYADEG
                     YYYECNNLTFENNVLIRIADWEI"
BASE COUNT          765 a          534 c          479 g          927 t
ORIGIN      
        1 tactactttt ataaatacca gaaataaaaa ttgtgaaaaa tttactaatt ctaattgtat
       61 taagttctat atcaatcgct ttgtatcaat atctccctgt agaaaaagat acagcaaaag
      121 ggcttgctgt tttgtttctg attggcgggc tttgggtatc agaggcactg cccattacag
      181 ttacggcact tgctgttccc ttaatagcgg ttttaacagg cattttcgac gctaaagatt
      241 cgtttaagca ttttgcaaac cctatcatat ttcttttcct gggcggtttt gcccttgcgg
      301 cggcgctcca taaacaaaat atggatgagc tgatagctaa aatagtatta aaaacttcca
      361 ggaacaatgt tttagtatct gcaataattt tatttttagt aacagctgtt ataagcatgt
      421 ggataagcaa tacggcaacc atggcaatga tgcttcccgt agcccttggg ctattgtaca
      481 gaataaacaa aatagaagac aatagaataa acctttttat acttttgggg cttgcgtatt
      541 cagccaatat tggagggatt ggaacaatag tcggaagccc tccaaacggt attaccgcag
      601 ctaatctggg catgaatttt aacgactggc tctatgtagg cataccatct gtattgattt
      661 tgcttccggt tgcagtttta ttcctctatc tatggataaa acctgttttt ctatacaagg
      721 aaacatctga tcaaatgtct attaattata gtgattattt taacaaaaac agcattttag
      781 tagttgtaat atttttcatt accgtgtttt tgtggctttt cagtaaaccc atatccggtt
      841 atctgcaaat caccaagagc tttgactccc tggttgctat atttgccctg attttactct
      901 gtgccaccag ggtagtgaac tggaaagaga ttgaagaatt tacagactgg ggcgtgctgc
      961 ttttgtttgg cgggggtatt gcacttagtg cagtgctctc cgagacaggg gccagcaagt
     1021 atcttgcaaa tatactgttt agcaatttgt ttgatttcgg ggtattgatt ttacttctgg
     1081 gcggtgtgct gcttgttgta tttcttactg aaattgcgag caacacggca attgccgcaa
     1141 ttttagtacc tattttctat gcagtaggtg ttgagatccc tactttaaat aacagtgtaa
     1201 tgccacttgc tgtaggaata gccgcatcct gcgcctttat gcttcccgta gctaccccac
     1261 caaatgcgct tgtgtttggt acaggaaaag ttagtcataa atcgatgatt aaaatagggc
     1321 ttggtcttaa cattctatgt gccgtgcttg tatgtttaat tacgtacctt gttatatgac
     1381 ctaagatttt aataattatg acaattttat ttccgtctaa atctcccagt cagcaattct
     1441 aatcagcaca ttattttcaa acgtaagatt attgcattcg taataataac cctcatcggc
     1501 ataacactca taaccccatt tttcataacc agagctgtta acaccagatt tcttttttgg
     1561 ttcaccaaga gtttttataa catcctctgt agtcatgcct gccataggaa tgcctctttt
     1621 tatactcttc tcaatctttt tcctgtcggt ttctattgta ggtttgttaa taaccggatc
     1681 aacatcaata tccgccgtct ttttttcgat ttctacctgc ttatttatct cagccgacaa
     1741 agtcccgcag ttccagacaa tcaccccatc tgtaatctcg gacacttggg agggcggttt
     1801 cattgagagc ggctttatga tatcgtttac atatactccg gtataagggt tttcggtatc
     1861 cagaaaatca gcgatttttg aaatacagtc ctgcttttcc cagtactgca taacagcgcc
     1921 aacgtttact ccatttgact taaggttctg ggctaaaacc gttttcttgt tttcatcatt
     1981 tttccattgc acatttttaa aagtgtgcct gtttccaata agaacaagca tggggctgtc
     2041 ccccacaagg ctattaataa tacgtgacat ccagacattc ttttcaatag gcactgtatc
     2101 aggcacatct actccaaata ctcttataca ccttccagct gctttaaaat ctttaaaact
     2161 tcttagcata ttcctatatt ctttgtgagt aacaaatgtg tttatcctaa tattggaaat
     2221 atcggtattg ccattgataa atccgtctat atttttttgc tcttcagagc ttatttcaag
     2281 accgacattt acgcagtttc ccatcgctgt aaatgcctca acaacatcag atacaagcct
     2341 tgcagactcg ggtttacccc ttttctctcc aaatattaat acgccgctgt tctcgagaag
     2401 agaaaaaatc ttctgctcat ttgcactgga ttgtatggta aatacaaaga ttaaaaaggt
     2461 tgatataatt aatgttttta gtcttatcat caaatgtccc atatgaatta aattatattt
     2521 attaatcctg ccgatagttt aaactaatct tcgcgttatt aataatatac ttcatattaa
     2581 cttagggcat tagacgttgt taggagtagt atatatgata aagagcaatg ataaagcggt
     2641 attcatggca attaacagtg ggaaaaatat cacgaatttt ttcacacgca aaatatggaa
     2701 aaaac
//
LOCUS       DBNGSV010000206         1549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1698520, whole genome shotgun sequence.
ACCESSION   DBNGSV010000206 DBNGSV010000000
VERSION     DBNGSV010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1698520"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC159U_07525"
     CDS             complement(<1..209)
                     /locus_tag="AC159U_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336946.1"
                     /translation="MHEINEDEEKRLLQEAVKLDATVFGLIFGLITGLIIFLATNWLI
                     LAGGHVGPDGKIVIGPHLQLLGQFF"
     gene            complement(223..>1549)
                     /locus_tag="AC159U_07530"
     CDS             complement(223..>1549)
                     /locus_tag="AC159U_07530"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336947.1"
                     /translation="IRGLSLFSALKNALLKQPAANKDVIKTLINSFHYPKMGPGMMWE
                     KVRDLVEQGLSTVHMKSGVDKIYWEDGKIVSISSNNEKSVSGTDFISSMPMRELVAKL
                     DPAAPKEIIEASQKLNYRDFITVALIIDKEDIFKDNWIYIHDPAVNVGRIQNFKNWSP
                     FMVPDPKKTCLGLEYFCFEDDNFWSMSDEKLIELAKEELQLLGFATKSQISDGTVVRM
                     PKAYPVYDDEYKAALNTVKGFLANICNIQLVGRNGMHKYNNQDHSMLTSILAVRNILG
                     AKYELWNVNADQDYHEEIKDRELVSSELSQISYTQPLVPSVFAVPRSENNVVEKAIIR
                     VFSRLDKFAFASAVGIASFIFIFFTTLFLDFKGSPEVVNSMLLLNNYFIGYDISIKGA
                     LIGGGYSIFWGFIFGWLFAYIRNLSLGFIIYNEKRKLENQSLKDLLDYI"
BASE COUNT          462 a          318 c          259 g          510 t
ORIGIN      
        1 ataaaaaact gtcccaaaag ctgcaggtgc gggccaatta ctatcttgcc atcagggcct
       61 acatgaccgc cagcaaggat taaccagttt gtggcaagaa aaattataag cccggtaata
      121 agaccaaata ttagtccaaa aactgtggca tcgagcttta cagcttcttg cagcagccgt
      181 ttttcttcgt cttcgtttat ttcatgcaat ttagtcaatc cctcaaatgt agtccagtaa
      241 atcttttaaa gactggtttt caagtttcct cttttcatta taaataataa atccaagtga
      301 taggtttctt atatacgcaa agagccatcc aaatataaaa ccccaaaata tactatagcc
      361 cccgcctata agcgctcctt ttatagatat atcatatcca ataaaatagt tatttagaag
      421 aagcatgcta tttactacct cggggctgcc tttaaaatct aaaaacaaag tagtgaaaaa
      481 gatgaagata aaacttgcaa tacccacagc cgaagcaaaa gcaaatttat caagtctcga
      541 aaataccctg ataatagctt tttctaccac attgttctct gatctgggta cagcaaatac
      601 tgatggcacg aggggctgtg tataggatat ttgagaaagt tcgcttgata caagctcacg
      661 atcttttatt tcttcatgat agtcctgatc ggcatttaca ttccaaagtt catattttgc
      721 gcctaatata ttcctgactg caagaattga ggttagcatt gaatgatcct ggttgttata
      781 cttatgcatt ccgtttcttc ctacaagctg tatattacat atatttgcta aaaatccctt
      841 aacagtattt agagcagctt tgtattcatc atcatatact ggatatgctt ttggcattct
      901 tactactgtt ccatcactaa tttgagactt cgtggcaaag ccaagcagtt gtagttcctc
      961 ttttgcaagt tcaattaact tttcatcact cattgaccaa aaattgtcat cttcaaaaca
     1021 gaaatattcc aggccaaggc aggtcttctt cgggtctggt accataaacg ggctccagtt
     1081 tttgaagttt tgtatcctgc ccacatttac agcggggtca tgaatataaa tccagttatc
     1141 tttaaatata tcttctttat caattattaa tgctactgta ataaaatccc tgtagtttag
     1201 tttctgagat gcttcaataa tctctttggg agcagcggga tcaagctttg caacaagctc
     1261 cctcatgggc atgctgctaa taaaatcggt gcctgataca gatttttcat tgttagaaga
     1321 aattgacacg attttgccat cttcccagta aattttatct actccacttt tcatatgtac
     1381 ggtacttaga ccctgttcaa ccaggtctct tactttttcc cacatcatac cggggcccat
     1441 ttttgggtaa tgaaatgaat ttattaaggt cttaataacg tctttattgg ctgcaggctg
     1501 ttttaggagt gcatttttta gtgcagaaaa cagagacagt cctcttatt
//
LOCUS       DBNGSV010000207         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1075298, whole genome shotgun sequence.
ACCESSION   DBNGSV010000207 DBNGSV010000000
VERSION     DBNGSV010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1075298"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC159U_07535"
     CDS             complement(<1..239)
                     /locus_tag="AC159U_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336948.1"
                     /translation="MKHHEQIIHKFEPRDNQKITINNTVDGFLSTGFHGQLLEMSLGK
                     TKCSLNVAEIMKKYNKLDRLLIICPKAIQSVWLEE"
     gene            complement(236..439)
                     /locus_tag="AC159U_07540"
     CDS             complement(236..439)
                     /locus_tag="AC159U_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3336949.1"
                     /translation="MNRAEQIKDLRKRHHLTQEKLADSLYGVTTQAVASWESGRRNCP
                     DPIWWQICLVWDKRDLWAEEHGE"
     gene            complement(440..760)
                     /locus_tag="AC159U_07545"
     CDS             complement(440..760)
                     /locus_tag="AC159U_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336950.1"
                     /translation="MNIHKFNIEYRQKHGFVVAIVDGEYIDTFETLIDAQDYVEDSLN
                     KILKEASTYNCDFCQEYFQEDEVEHRIEDQRFTAPLGSSWTLGGDVGSVPVCPICGDD
                     LEDC"
     gene            complement(1018..1200)
                     /locus_tag="AC159U_07550"
     CDS             complement(1018..1200)
                     /locus_tag="AC159U_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336951.1"
                     /translation="MKDDEIRKKFDDFVAEKLVERKPDTRPEPCEKCDGEDFECMKCN
                     AKREWIKEQSKLKDTK"
     gene            complement(1298..1618)
                     /locus_tag="AC159U_07555"
     CDS             complement(1298..1618)
                     /locus_tag="AC159U_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336952.1"
                     /translation="MKVQIKDVIKKHKMSRATIMKRCKALGFPVYTIMAAGSKHMAKA
                     MDEENVEAVVKFNPENATVPGVYLSIEEKTKAYKKHSFSKQGVSMTRPSSIAHRLTYY
                     GGIK"
     gene            complement(1615..1950)
                     /locus_tag="AC159U_07560"
     CDS             complement(1615..1950)
                     /locus_tag="AC159U_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336953.1"
                     /translation="MKELIKGIAALLIVIVGTTLLIVLIWSISISFNSKQEPQPQYSI
                     YEYSYDNVAVFRYKDHKVIYEFAPSGIHLIQLIDGAKQITILDFGTKEQSIDFAYAYL
                     ESIQREDRL"
     gene            complement(1947..2240)
                     /locus_tag="AC159U_07565"
     CDS             complement(1947..2240)
                     /locus_tag="AC159U_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336954.1"
                     /translation="MKVYEQWKIQELKEKKMLEQRGVCAGCGKEFRIGDILELAHRIP
                     QRKYLIKLYGKDILHHELNMELTHSGYCNSACQISPNKTELVNELVEKIRETL"
     gene            complement(2227..>2517)
                     /locus_tag="AC159U_07570"
     CDS             complement(2227..>2517)
                     /locus_tag="AC159U_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336955.1"
                     /translation="AGFEGFVYIDMVLVGDNFDVSHYKMYDVKNKSDIPQHVLNDPNM
                     KDMIRGKLKSWSNYSLKERKQTIDNVINEMIQCGINTAKFQEIMKGLNHEGV"
BASE COUNT          675 a          569 c          351 g          922 t
ORIGIN      
        1 atctcttcaa gccatactga ttgaattgcc tttgggcaga taataagaag tcggtccagt
       61 ttattatact tcttcataat ctctgcaaca ttcaatgaac attttgtctt acccaaactc
      121 atttcaagca attggccgtg aaaccctgta cttagaaaac catccactgt gttattgatc
      181 gtgatttttt ggttgtctct cggctcaaac ttatggatta tctgttcatg gtgtttcact
      241 ctccatgctc ctctgcccat aagtctcgtt tatcccatac aaggcaaatc tgccaccata
      301 tgggatctgg acaatttctt cttccagatt cccatgatgc caccgcttgt gttgtcacac
      361 cataaagaga gtccgccaac ttctcttgag taagatggtg ccgtttcctt aaatctttaa
      421 tctgttctgc tctgttcatt taacaatcct ccaaatcatc accgcatatt gggcaaaccg
      481 gtactgatcc aacgtcaccg ccaagggtcc acgaacttcc taatggagct gtgaaacgtt
      541 ggtcctctat tctatgttcc acttcatcct cctggaaata ttcctggcag aaatcacaat
      601 tgtatgtaga tgcttctttc aggatcttgt tgagtgagtc ttctacataa tcctgtgcat
      661 ctataagagt ttcaaatgta tcaatatatt caccatcaac tattgccacc acgaaaccat
      721 gtttctgtct gtattctata ttaaacttgt gtatgttcat ttctcttctc ctgtatatgt
      781 tgtattgaat caagactgta attagtcccg gataatgtaa taatattaaa gtcttggagt
      841 gcaatcataa aatcaagtaa gttacttatc ccttttgtag tgtaatcgtg ctgcttatca
      901 tcaagtgttg ttatctgaaa aatcacttga accacctgtc aagaatcttt actgccagat
      961 caaccataaa catggcaatg tctttacaca acatgtacca ccaatactga aacttgttca
     1021 ttttgtatcc tttagttttg attgctcctt tatccactct cttttagcat tacacttcat
     1081 gcattcaaaa tcctcaccat cacatttttc acaaggttct ggacgtgtat caggttttct
     1141 ttctaccaac ttctcagcta caaaatcatc aaacttcttt ctaatctcat catctttcat
     1201 ttcttcactc ccaactgttc agcaagccat tttcttgccc attggcaatt cacacaatca
     1261 attccttcac catcgcatct gtcacaagga tcttcactca ttttattcct ccataatacg
     1321 ttaatctgtg ggcaattgaa gagggtctgg tcatacttac accctgttta ctgaaactgt
     1381 gctttttata tgctttagtc ttttcttcaa tagataaata tactccgggt acagttgcat
     1441 tctccggatt aaactttaca actgcctcta cattctcttc atccatagcc tttgccatgt
     1501 gcttagagcc tgctgccata atagtgtata ccgggaaacc taatgcttta catcttttca
     1561 taatcgtggc tctgctcatc ttatgcttct ttatcacatc tttaatctgt accttcataa
     1621 ccggtcctct ctttgtattg actctaagta agcataagca aaatctattg attgttcctt
     1681 agtaccaaaa tctaaaattg ttatttgctt tgccccatct atcaactgta ttagatgaat
     1741 cccggacggg gcaaactcat atatcacttt atgatcttta tatctgaaca ctgctacgtt
     1801 atcataacta tattcgtaga ttgaatattg gggctgtggt tcttgtttac tattaaagga
     1861 tatactaata gaccatatca gtactatcag taatgttgtt cccactataa ctatcagtaa
     1921 tgctgctata ccttttatta gttctttcat aatgtctccc ttattttttc aactaattca
     1981 tttactaatt ctgttttgtt tgggctgatt tggcaagcac tattacaata tcctgaatgt
     2041 gtcagctcca tattaagctc atggtgcaga atatccttac catataattt tattaaatat
     2101 ttccgttgag gaatccggtg agcgagttct aatatatctc caatacgaaa ctctttgcca
     2161 caaccggcgc aaactccacg ttgttctaac atcttttttt cttttaattc ttgtatcttc
     2221 cattgttcat acaccttcat gatttaaacc tttcattatc tcctggaatt tagcggtatt
     2281 tattccgcat tgaatcattt catttattac attgtcgatt gtttgtttac gttcctttag
     2341 tgaataattt gaccatgact ttaattttcc tctaatcata tctttcatat taggatcgtt
     2401 caaaacatgt tgcggtatat cagatttatt ttttacatca tacattttgt aatgagatac
     2461 atcaaagtta tcaccgacta aaaccatatc aatatagaca aacccttcaa accctgc
//
LOCUS       DBNGSV010000208         4569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1351653, whole genome shotgun sequence.
ACCESSION   DBNGSV010000208 DBNGSV010000000
VERSION     DBNGSV010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1351653"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="AC159U_07575"
     CDS             <1..605
                     /locus_tag="AC159U_07575"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF014788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046084 - adenine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3336956.1"
                     /translation="RKDEFDIAGFVVGVVDKKNIIDGKNVKNGDVLIGLASSGLHSNG
                     YSLARNLLLKKLKYKLYDKPRPLRKSLGSELLTPTKIYVNTIESLNKKFNLKAIAHIT
                     GGGLIDNIPRVIPDKLKVQIDTKSWTRPPIIKLIQNSDMVEPSELLRTFNCGIGMVIV
                     VDSVESSKILAQLKKLNEKAFIIGQVAKRNKNESTVEFVN"
     gene            627..1646
                     /locus_tag="AC159U_07580"
     CDS             627..1646
                     /locus_tag="AC159U_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336957.1"
                     /translation="MYLKKIIFFTAVLLFLICTSSNLYSQNKYSPKYKTLAHEIISKE
                     PELVSKDQAYRKLDILIDNARKVLKKREKYRRWQTEEIFKTIYMDLLNQGYDYKSNDF
                     LSYGLVNGKLDCDNYTLIYYAIAQVIGFPIYIVNLPKHTTLLWDTDGRHDPINKSNPV
                     NEGDFYWETTLGEIRTDEKYITTRRIEKSSINSGVFLNKVKNKNMYAVMHNYIATMHI
                     ESGEFDKALAEISKAVELDSKFPEAFNNMGIIYSNLNDIVSAVKSYDKALSLHPNFTL
                     AYINKGIALQKSKDFHGAISLYTDALSIESDFRPLYLMRSKLYKKVGLYDKALKDIET
                     YESMK"
     gene            1742..2662
                     /locus_tag="AC159U_07585"
     CDS             1742..2662
                     /locus_tag="AC159U_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3336958.1"
                     /translation="MKHKIFNFLYTIEGERKAIIYMIAGGFCFATMGALTFRLGKEVD
                     WVFIAFCRMLFSFVLVFFLASKSGNTPILLTRKLLWLRSLTGTIAMLATFYSFTHLPV
                     SDVSAITETRPVWVALLAVVLLGEKLNKVLLPVTILSIIGVLLIEKPHFDQRSFAVFV
                     TLVASVLGAVVMICLRKLRDLDPRTIVTHFSMTATLGSIVFMYLFRDINSYENYFQGH
                     IILYLVGIGLFGTLGQLTMTKAFALGRASIVSSTGYAKVGFSAIYDLVLFSAVFELYT
                     IAGMLLILGASTWLINSSNNVQDTQGRLIK"
     gene            2905..3219
                     /locus_tag="AC159U_07590"
     CDS             2905..3219
                     /locus_tag="AC159U_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3336959.1"
                     /translation="MERRGSLRLKLQERAQIGESGKDYYEAVSYDISETGISVKSQDF
                     IPEKTPINVILDHSNKALNLEGVIAWVSGKSSSNQYKMGIKITNPSGELTNIYNYLSR
                     IA"
     gene            3498..3731
                     /locus_tag="AC159U_07595"
                     /pseudo
     CDS             3498..3731
                     /locus_tag="AC159U_07595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
     gene            3820..>4569
                     /locus_tag="AC159U_07600"
     CDS             3820..>4569
                     /locus_tag="AC159U_07600"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3336960.1"
                     /translation="MSIDINSTIGWIGTGVMGESMCGHLINAGYKVHVYNRTQSKAQN
                     LVDKGAVWQNSPADVAKRSDVVFSIVGYPDDVRDVYFSDNGVFQALNKGSFIVDMTTT
                     EPSLAVEIYNKAKEMGSTAIDAPVSGGDIGAKQGMLSIMVGGDKFAVDMIMPMFETMG
                     RYIVYQGPAGSGQHTKMCNQIAVAGVMVSTCETLLYAHKAGLDIETVLSSITKGAATS
                     WTLENLAPRIVRRDFKPGFYVEHFIKDMKIAL"
BASE COUNT         1526 a          679 c          871 g         1493 t
ORIGIN      
        1 atagaaaaga cgagtttgat attgctggtt ttgtggttgg tgtagttgat aaaaaaaaca
       61 taattgacgg taaaaatgtt aaaaacggtg atgtattaat tggccttgca tcaagcggtc
      121 ttcattctaa tggctattca cttgcaagaa atttgctact aaaaaagctt aaatataaat
      181 tgtatgataa accaagaccg ttaaggaaat ctcttggcag tgagcttcta acgcctacta
      241 aaatatatgt taatacgatt gaatctttaa ataagaaatt caatttaaag gctattgccc
      301 atattacagg tggtgggtta attgataata ttccaagggt aataccggat aaattaaagg
      361 tacagattga tactaaatct tggacaaggc ctccaataat taagcttatc cagaattcag
      421 atatggtaga accgtctgag cttttaagaa cattcaattg cggaattggc atggttatag
      481 tcgtagattc tgtagagtct tctaaaatcc ttgctcagtt aaagaagtta aatgaaaaag
      541 cttttataat cggccaggtg gctaaaagaa ataagaatga aagtacagtg gaatttgtta
      601 attaatccca ggtatgatct gtttatatgt atttaaaaaa aatcatattc tttacagctg
      661 tattattatt tctaatttgt acttcttcta atctttattc tcaaaacaaa tatagcccta
      721 agtataaaac cctcgcacat gagattatat caaaagaacc tgaattagtc tcaaaagatc
      781 aggcctacag aaaacttgat atcctaattg ataatgcaag aaaagtacta aagaaaagag
      841 agaagtacag gcgctggcag actgaagaaa tatttaaaac catatatatg gatcttttaa
      901 atcagggata tgactacaag agtaatgatt ttttaagcta tggtcttgta aatggaaagt
      961 tggactgtga taattatact cttatctatt atgcaattgc ccaggttata ggatttccaa
     1021 tttatatagt taacttacca aagcatacca ctcttctctg ggatacagac ggcaggcatg
     1081 atcccataaa taagtcaaat cctgtaaacg aaggggattt ctattgggag accacccttg
     1141 gtgaaataag aacggatgaa aagtacatta ctacaaggcg cattgagaag agttcaatta
     1201 acagcggagt attcttaaat aaggttaaaa ataaaaacat gtatgctgtg atgcataact
     1261 atattgctac gatgcacata gaatctgggg agtttgataa agcccttgct gaaataagta
     1321 aggctgtaga acttgattcc aaatttcccg aagcttttaa caatatgggt atcatatatt
     1381 caaatctcaa tgatattgta agtgctgtta aaagctatga taaagcatta tcactacatc
     1441 ctaactttac acttgcatat attaataagg gcatagcgct gcaaaaatcc aaagactttc
     1501 atggagcgat ttcactatat acagatgcac taagtataga atctgatttc aggcctcttt
     1561 atttgatgag atcaaaattg tacaaaaaag taggtttgta tgataaagct ctaaaagata
     1621 ttgagaccta tgagtcaatg aaataatttt tattttttca accgttccaa aaaatatcct
     1681 aaactacaat cctgcagcag gtaatatagg tgattttaat cgataatttt atgtcctacc
     1741 tatgaagcat aagatattta attttctata taccattgaa ggagaacgca aggcaataat
     1801 ctatatgatt gccggtgggt tttgttttgc aacaatgggt gcactgacgt ttagactcgg
     1861 taaggaagtt gattgggtat ttattgcatt ttgcaggatg ttgttttcat ttgttctcgt
     1921 atttttttta gcttctaaat caggtaatac gcctatactg ctaaccagaa aactgctatg
     1981 gcttagaagc cttacaggaa ccatagctat gcttgctaca ttttattcat ttactcacct
     2041 gcctgtatcg gacgtatctg ctatcactga gacacgtcct gtgtgggtgg ctcttctggc
     2101 agtagtactt ttaggtgaaa agttaaataa ggtcctgcta cctgtaacta ttttaagcat
     2161 tattggtgta ttgcttatag aaaaaccaca ttttgatcaa agaagttttg ctgtatttgt
     2221 aactctcgtt gcatctgttt taggagctgt tgttatgata tgtcttagga aactgagaga
     2281 ccttgatcca aggacaatag ttacacattt ttcaatgaca gctactttgg gatcaatagt
     2341 gtttatgtat ttatttaggg acataaattc atatgaaaac tatttccagg ggcatattat
     2401 attatattta gttggtatag gtctattcgg tacattgggt cagctgacaa tgaccaaggc
     2461 atttgctctt ggtagagctt ctattgtgtc atccaccggc tatgcaaagg taggattttc
     2521 agcaatatat gatctcgttt tattcagtgc tgtttttgag ctttatacaa tagccggaat
     2581 gttacttatc cttggcgcat ccacatggct tataaacagc tcaaataatg ttcaagatac
     2641 acagggcagg ttaattaaat agaattttaa attatataag ttaaaaattc ttactaacaa
     2701 ttaaaatttt tattaatgaa tgttaaatat gttcagtata tttattaaat atattttttt
     2761 tgatattatg ttatattaaa gattgaaatt tattatattt agatgtggtt acatatcata
     2821 attaaattaa aacattatta ttgacaaaaa gatcatatag actatagtag tagcatgagg
     2881 gaggataatc taaaattgtc agaaatggag cgtaggggat ctttgagatt aaagttgcag
     2941 gagagagcgc aaattggtga atctggaaag gattattatg aagcggtctc ttatgatata
     3001 tcagaaacgg gtatatcagt aaaatcgcaa gactttattc cagaaaaaac tccaattaat
     3061 gttatcttag atcacagcaa taaagcgctt aaccttgaag gagtaattgc ctgggtatca
     3121 ggtaaaagta gctctaacca gtataaaatg ggtatcaaaa ttaccaaccc aagtggagaa
     3181 ctgacaaata tttataacta tctatcaagg atagcctaac ttatcgaaag cattacctaa
     3241 tattaaaaca tataataatt taaataactt tagtattaat agtttttaag cattaattgt
     3301 atgttccttt aatactttat catgttttta tcgttcaact tgaaatgtgg ttgtattcca
     3361 ttgtcttgta ttatatattc ttagtatact caataagtaa taaggcagat attggtggag
     3421 caaagaacct catataggat accttttgaa gaaaaagtgc agtatggcta cttaagccgg
     3481 catattttga tggaactctt tcaaatatat cagaaaatgg tattgccgta aaagctgaca
     3541 atcctattcc cgaaaatact gctgttacag tgcttgttta ctatctcaac aaaccaattg
     3601 agttagaagg tgaggtgaaa agactctctc agtgttattt tacaaataag tttgagatgg
     3661 gtattagcct tactgttagt aatagtgagt ataactcaat ttacttatct atgcttagcc
     3721 gtagtttata aaaaactttt cattttcctt tctttgtagt agattttttt atattactta
     3781 ttttcctatt tttaagcaca tatttattgt ggggttttta tgagcattga tataaattca
     3841 actatagggt ggattggcac aggagtaatg ggtgagtcca tgtgcggaca tctaataaat
     3901 gcagggtaca aagttcatgt ttataacaga acacagagca aggctcaaaa tcttgttgac
     3961 aagggagcag tttggcaaaa ctcgcctgca gatgtagcaa aacgctctga cgtagtgttt
     4021 tcaatagttg gttatcctga tgatgtaagg gatgtttatt tttcagataa cggtgtattt
     4081 caagctctta ataaagggtc atttattgta gatatgacaa ctacagagcc atcacttgct
     4141 gtagagatat ataataaagc aaaagaaatg ggatctactg caatagatgc acctgtgtcg
     4201 ggcggggata ttggtgcaaa acagggaatg ctctcgatca tggtaggcgg agataagttt
     4261 gctgtggata tgataatgcc tatgtttgaa accatgggaa ggtatatagt ctatcagggc
     4321 ccagcaggct cgggacagca tacaaagatg tgcaatcaaa ttgctgttgc gggggtgatg
     4381 gtttcaacat gtgaaacact gctttatgct cataaggccg ggcttgatat tgaaactgta
     4441 ctctcttcca taaccaaggg ggctgcaaca tcctggacgc ttgaaaacct agctcccaga
     4501 attgtaagaa gagattttaa gccaggattt tatgtagagc attttatcaa agacatgaag
     4561 attgcactc
//
LOCUS       DBNGSV010000209         2460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1491758, whole genome shotgun sequence.
ACCESSION   DBNGSV010000209 DBNGSV010000000
VERSION     DBNGSV010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1491758"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..556)
                     /locus_tag="AC159U_07605"
     CDS             complement(194..556)
                     /locus_tag="AC159U_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR3336961.1"
                     /translation="MIKFVMCLTRHPDMTREQFKDYWMNSHGPFFMSNADAMGAKKYV
                     QSHTMDTPLNEGIRASRVMQPEYDGVAEVWFESEQALMEGMSSPEGQKLGAALLEDEK
                     NFIDHSKSCAFIVKEYEF"
     gene            complement(825..1859)
                     /locus_tag="AC159U_07610"
     CDS             complement(825..1859)
                     /locus_tag="AC159U_07610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3336962.1"
                     /translation="MPYKNNLTLATFNLYNLQLPGKAMYNGIRYTPQQYREKLEWTAN
                     KLKLLDADIIAFQELWRPECLRDVFVTANLDDEYTLIAENINSKGMDNAIAVRKPHEV
                     VKAEWIKNFPEETVLKKRKGRSSDPDYQISVNISRFSRAVLSVTLNVIPTAGAEPVRV
                     RVLVAHLKSKKCIMLDKQEKCDPSIRPHATALGQAMSSIRRTAESAAIRVLVNKMMRS
                     CDDPVIVLGDLNNTEHSVSTAIIAGQPKFYMYTNNCSASRSDRGLYSTSYMQGYRSVK
                     DTVFTHITDGNMETLDHILVSKQFYDYSDNRLWSFNNLRCFNDYLADKNDVSSDHAIV
                     SATFDYNPSN"
     gene            complement(2174..>2460)
                     /locus_tag="AC159U_07615"
     CDS             complement(2174..>2460)
                     /locus_tag="AC159U_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336963.1"
                     /translation="NDFLQTGADELPAQIVGHSYIFESASAVLFPKQEIADNPKRFVE
                     IHAKEDPGHSQAIRRTVRKIEPDLSTEEKKKIVNFSKQSGRYFIDLFSQI"
BASE COUNT          720 a          505 c          415 g          820 t
ORIGIN      
        1 catagggttt attacctaaa attctaactt gtaatctggt actattatag gggggaaggt
       61 caattggcag actggaatta tatacagttc tagtaatatt cactcccatg ttctggacaa
      121 agtaaaaaaa tttaaaactg atggcatctg gtcttaacat tgaaaactaa tattcactac
      181 ttcattgtgc cacttaaaat tcatattctt taacaataaa agcacatgat tttgaatgat
      241 ctatgaaatt cttctcatct tctaaaagtg cagcgcctag tttctgacct tctggagaac
      301 tcatcccctc cattaacgct tgttctgatt caaaccatac ctctgcaact ccgtcatact
      361 ccggctgcat tacccttgaa gctctaatac cttcatttaa aggggtatcc attgtatgtg
      421 actgcacata cttttttgca cccattgcat cggcgttgct cataaaaaac gggccatggc
      481 tgttcatcca gtagtccttg aattgctcac gagtcatatc agggtgtctg gttaaacaca
      541 taacaaattt gatcatttca atactcctca tttcacaaga tgtttgagta tataactgta
      601 cattattctg atccactgac cagtatagtt aaaaaaacaa attctgttaa tatttatgat
      661 ttcaactctg acttaattaa gctttaaggt tggcaactta aatattgtta gatacttctt
      721 ctataataac taagtactaa atcattattt attatatttt gaaatgaagt attagcattt
      781 tggttccacg cataaaccaa aataccaaag cctgacccca tgcgttaatt agacgggtta
      841 tagtcaaatg ttgcgcttac tatcgcgtgg tccgaagata catcattctt atcggcaagg
      901 taatcattga agcatcttag gttattaaaa ctccagagcc tgttatcaga atagtcataa
      961 aactgcttag atacgagaat gtgatccagg gtttccatat tcccatctgt gatatgagta
     1021 aagaccgtgt ctttaacact tctgtatccc tgcatataag aagttgaata caatcccctg
     1081 tcgcttctac ttgcactgca gttatttgta tacatataaa atttgggctg ccctgctata
     1141 atagctgttg acacactgtg ttcggtgtta ttcaggtctc caagtacaat tacaggatca
     1201 tcgcaacttc tcatcatttt gtttactaaa actcgtattg ctgccgattc cgctgttctg
     1261 cgaatactcg acattgcttg cccaagagca gttgcatggg gtcttatcga cggatcgcat
     1321 ttttcctgtt tatcgagcat tatacatttc tttgatttta agtgagcaac taagaccctc
     1381 actcttacag gctccgcccc agctgtggga ataacattta aagtaacgct caatactgcc
     1441 cttgaaaacc tgctaatatt aacgctaatt tgataatccg gatcactgga acggcccttg
     1501 cgcttcttaa ggactgtttc ttcagggaaa ttcttgatcc attcagcttt tactacctcg
     1561 tgaggcttcc tcactgctat ggcattatcc atccctttag agttaatgtt ttcagcaata
     1621 agtgtgtact catcatccaa attagccgta acaaatacat ccctcaaaca ctccggtctc
     1681 catagttcct gaaatgctat gatatcagca tcaagcagtt ttagtttatt agcagtccac
     1741 tcaagcttct ctctatattg ctgcggagtg tatcttatgc cattatacat agcttttccg
     1801 ggtagctgca gattatataa gttaaaagta gctaaagtca gattgttttt gtatggcata
     1861 atttcctctg gggcttattc cttaatcaat tttttatgta taacgctata aactgcctat
     1921 ctagttaatt aattttctcc cataaattct ttgcagatat ccattaaatt aatgctagta
     1981 aattcggata tggttgtaaa gatatgaata agtcactatt gaaaaaaagt gatagtgacg
     2041 aatgtgtgac cttttgggat attttggagg gagatattgt ataattatca aaaatcaggg
     2101 aattaaatcc cgaaattagc ctacaaacca aaacatcaaa gcctgacccc aatgcctggt
     2161 taggttgagg aagttatatc tgactgaata aatcaatgaa gtaacggccg ctttgtttgg
     2221 aaaaatttac tatcttcttt ttttcttctg tacttagatc aggctctatt ttcctcacgg
     2281 tccttctaat ggcctgggaa tgaccagggt cttcctttgc atgaatttct acaaaccttt
     2341 ttggattatc agcaatctcc tgctttggaa aaagcactgc tgaggcgctt tcgaaaatat
     2401 aggaatgccc cacaatctga gctggaagct catctgcacc agtttgcaaa aaatcattat
//
LOCUS       DBNGSV010000210         6335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1907645, whole genome shotgun sequence.
ACCESSION   DBNGSV010000210 DBNGSV010000000
VERSION     DBNGSV010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1907645"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC159U_07620"
     CDS             complement(<1..455)
                     /locus_tag="AC159U_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR3336964.1"
                     /translation="MNIKKNSRQMYLSLLAGLFGFSLLITSCAPIDPYPSSQRTYEGA
                     AAGAALGSVAGLLIDKDNRWRGAMIGGLLGAALGGTVTEVSQRASREAAAEGRPVAYE
                     SKDGFQRVEATPVAYNANTDCHKVRERIWQDGELVKDEVKEVCDSNKSEP"
     gene            695..3613
                     /locus_tag="AC159U_07625"
     CDS             695..3613
                     /locus_tag="AC159U_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HIR3336965.1"
                     /translation="MQKNLYSKSRNILTTIIFILAVALVFLLNTSLKFSASGHGTQTE
                     ITSSVLSYVNKYYIDKSKIDPVKMLEQSLAELEQIIDEVLIDFNNDSKKFIVQVNDNK
                     KTFDYSNVNDIKDLPDMLDNVLSYVAMHHKPQNNTFLEIEYAVVDALLKTLDSNSGII
                     TPKIYKEFMIETEGSFGGLGIVIGIREGQLTVISPIEGTPAYLAGVKSKDKIVQIEDG
                     STVNMSLLEAVSKLRGPKGTKVNIHVMRDGFAIPKKFTIKRDTIKIESVDAFLLSDNI
                     LYLKVRDFQKNTLSSIKEKANKLTSDNINSVNGIILDLRGNPGGLLDQSKKISDLFLQ
                     NGNIVSTQIGTDKNTYSANDEYYEYRGKLIVLVDSGSASASEIVAGALKNNNRALIVG
                     DQTFGKGSVQQVFSLKDNAALKLTIANYLTPGDISIQDIGITPDIFLQPVRVSKDAID
                     LFTNENQDSNKDNNINYSQNPKKSIKFIEALPDPDQEEDMVEEALSKQEKLSKIQEDF
                     AVIFSRNIINNSSSPQTITQAGFIESELEKYTKQQQDQITLELNELDIDWTNTDKTNN
                     ENLFTVTVSKSKNNVNAGDIIDIGVTVKNTGSKSTERLYAVTKSDNPVFNDKEYIFGK
                     IPAGNSKTWTNSFEVPKWSISRNDKVELIFKSNNDEQILSKNFDVQTIQQQRPAYAFN
                     YELIDDGRFETKGNSNSIAEEGELVGLRLKLKNTGSGESEKTTVLLKNNLGEDLYLKK
                     GRAKIENLKPNEVREELFLFSVNNPINNLDVEIQIFDDNYREGVTEKIKLDSTETSND
                     KIKELTAWYKVNADNVPIYGSPSEKSSIVAYASKDSLFKSDISNNEWAKVSLDSSSRG
                     WINMKFLTTTDKKVTEANITITSSFQQPPQIKLDQPPLLIKNNEISLKGIVQDSDGVR
                     LITVYLGEDKVYLKNFYSQSAAVDTKISLKSGINYINIFAKDKLGLISKKTYIVRYDA
                     "
     gene            3641..4573
                     /locus_tag="AC159U_07630"
     CDS             3641..4573
                     /locus_tag="AC159U_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3336966.1"
                     /translation="MIKILASRLFTGLFVVLTVISITFFLLRSLPGGPFDTEKKLPAQ
                     IQINIEKKYNLDQPLWKQYSIYLYNICRGDFGPSYKYVDRSVNDIIKQTFPVSLKLGI
                     VSLILSLLLGCVVGIFSSIKRGRSFDFLSFSFSTALISVPSFVIGAFLIYIFSIKYGI
                     LPAALWGEPKHYILPGLTLAAGPAAYLMRLVRSSMLDVTGAQFIRTAKAKGLKTYKII
                     FKHIFKNALIPIVTVLGPITAYLVTGSFVVEHIYAIPGMGKFFVLAVSNRDYPLIMGI
                     TIVYTVVLVVSNLIVDMLYLKLDPRIRFGISTDK"
     gene            4570..5313
                     /gene="truA"
                     /locus_tag="AC159U_07635"
     CDS             4570..5313
                     /gene="truA"
                     /locus_tag="AC159U_07635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3336967.1"
                     /translation="MRNIKLFIEYDGTNYHGWQRQPGLNTIQKLIEESIQKITNEKTI
                     ITGSGRTDSGVHAMEQVANFKTSSTINTDRITKALNSVLPCDIAIIKSQQVHIDFHSQ
                     YDSLRKTYIYKILNRQSRTAINTKRVWHIPYKLDLKKMQNAAASLLGEHDFLVFSKTG
                     SGVKTTVRSIYKASFTKKRDGTIEFCIQANGFLKGMVRLITGALVSVGKGKMTESEFA
                     EIISSGKKHKYIKSAPAHGLYLAKVAYDS"
     gene            5349..6107
                     /locus_tag="AC159U_07640"
     CDS             5349..6107
                     /locus_tag="AC159U_07640"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3336968.1"
                     /translation="MRICRFINTNSQTCYGIIENTTVTEIDNSPFEDYSITDKSYDLD
                     ELKLLAPCVPSKIVAIGLNYKDHAAEMNKKIPEDPMLFLKPGSSVIGNEDKIIYPAHM
                     SKRVDYEGELAVIIKRVAKNVSMENAMDFVLGYSCLNDVTARDLQSKDIQFTRAKGFD
                     TFAPIGPWIETDIDPTNLDIKTFLNGELKQNSSTSNLIFNIPKLINFISHVMTLYPGD
                     VISTGTPGGIGPMKIGDKVGITIQSIGTLTNYVG"
     gene            complement(6111..>6335)
                     /locus_tag="AC159U_07645"
     CDS             complement(6111..>6335)
                     /locus_tag="AC159U_07645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3336969.1"
                     /translation="EKYKKALQVLLEVDKRSKYNRRLHLLVAEVYLRLGDFKQAHKFA
                     DRSLKSGKAKLDKKQRERAEYILNYTERYQ"
BASE COUNT         2229 a         1121 c         1140 g         1845 t
ORIGIN      
        1 ggctcggatt tattggagtc gcaaacttct tttacttcat cttttacaag ctccccatcc
       61 tgccatattc tttctctgac tttatgacaa tcagtgttgg cattataggc tacaggagtt
      121 gcctcgactc tctgaaatcc gtccttactt tcatatgcca cgggtcttcc ctcagcagca
      181 gcttctcttg atgcgcgctg tgaaacttcg gttaccgtac cacctagcgc cgctcctagc
      241 aggcctccaa tcattgcacc ccgccacctg ttatccttat caataagaag ccctgcaact
      301 gaaccaagtg cggctcctgc tgcagccccc tcatatgttc tttgacttga aggataagga
      361 tctatgggtg cacaggatgt aattagcaag ctaaaaccga atagaccggc aagtaatgat
      421 aaatacatct gcctgctgtt ttttttaata ttcataattc aacctcccct gtttttaaaa
      481 ctataaacct tattatatta aaaaggtaac aagttatttt aaaaagaaaa cccctctata
      541 ttattagcag tttgatctca ttaatcagtt atcctattta caagtttatt aaatgcttta
      601 gcttatttgt aaaaatcaat attaaacaat ataattccga agtttttatt ttatactcta
      661 tatatatgca taaataattt ttctggagaa tacattgcaa aaaaatcttt attcaaaatc
      721 acgcaatatt ttaactacaa taatttttat acttgctgtt gcacttgtat tcttactcaa
      781 tacttcactc aagttttcag cttcgggtca tggcacgcaa accgagatta catcatcagt
      841 attaagctac gtaaacaaat actatatcga caaaagcaag attgatcctg taaaaatgtt
      901 agagcagtcc cttgcagagc ttgagcagat tatagatgaa gtgttaattg attttaacaa
      961 tgactctaaa aaattcattg ttcaggtcaa tgataataaa aagacttttg attactcaaa
     1021 cgtaaatgat ataaaggacc ttccggatat gctggataat gttttgagtt atgtggccat
     1081 gcaccacaaa ccgcaaaaca acactttttt ggaaatagaa tatgcagttg tagatgcatt
     1141 actaaaaaca cttgattcaa actcgggaat aatcactcct aaaatttata aagaatttat
     1201 gattgaaacc gaaggcagtt ttggcggact tggtattgtc ataggtataa gggaaggaca
     1261 acttactgtg atatctccca tcgaaggcac gccggcatat cttgcaggag taaaatcaaa
     1321 agacaagatt gttcagatcg aagacggttc tacggtcaat atgtctttac tcgaagcggt
     1381 aagcaagctt agaggaccta agggcacaaa agtaaacata catgtgatga gagacgggtt
     1441 tgcaattcca aagaaattta ccatcaaaag ggatacgata aaaattgaaa gcgtcgatgc
     1501 atttttactt tcagacaata tcctgtattt aaaggtaagg gattttcaga aaaacacgct
     1561 tagcagcata aaagaaaaag ctaataaact taccagtgac aacatcaatt ctgtaaacgg
     1621 tattattctt gatctgcgtg gaaaccccgg gggactgctc gatcaatcaa aaaaaatatc
     1681 agatctattt ttacaaaatg gcaatattgt ttccacacaa ataggaaccg ataaaaacac
     1741 atacagcgcc aatgatgagt attatgagta cagaggaaag ctcatagtcc ttgttgattc
     1801 aggaagcgca agtgcatcag agattgtagc gggagctctt aagaacaata acagggcgct
     1861 tatagtaggt gaccagacat ttggcaaagg ctccgtgcag caggtattta gcctaaagga
     1921 taacgctgct ctaaaattga ccatcgccaa ctacttaacc cccggagata tatccattca
     1981 ggatattggt attactccgg atattttttt acaacccgtt agagtatcaa aagatgctat
     2041 tgacctcttt acaaatgaaa accaagactc taataaagac aataatatca attattctca
     2101 aaatccgaaa aaatccatta aattcattga agccctgcct gaccctgatc aagaagaaga
     2161 catggtagaa gaagctctta gcaaacaaga aaaactaagc aagatacaag aggattttgc
     2221 agttattttt tcaaggaaca ttattaataa ctcctcaagt ccacaaacca ttacgcaagc
     2281 tggttttatt gaaagcgaat tagagaaata tacaaaacaa cagcaagacc aaattaccct
     2341 tgaattaaat gagctggata tagactggac aaatacagac aaaactaata atgaaaattt
     2401 gtttaccgta acagtgagca aatcgaaaaa taatgttaat gccggagata ttatcgatat
     2461 tggcgttaca gtcaaaaaca ctggcagcaa atcaactgag agactatacg cagtaacaaa
     2521 atccgacaat ccggttttta atgataaaga atatatattt gggaaaatcc ctgccggaaa
     2581 ttccaagacc tggacaaaca gctttgaagt tcccaaatgg tctatttcga gaaatgataa
     2641 agttgaactg atttttaagt ctaataatga tgagcagatt ctgagcaaga attttgatgt
     2701 acaaacgata caacaacaaa gacccgcata tgcttttaac tatgaactga tagacgatgg
     2761 gagatttgag actaaaggaa acagcaatag tatagcggaa gagggagagc ttgttggtct
     2821 tagattaaaa ctaaaaaata ccggaagcgg ggaatccgaa aaaactactg ttcttcttaa
     2881 aaacaatttg ggagaagacc tgtatctaaa aaaagggcgg gcaaaaattg aaaacttaaa
     2941 accaaatgag gtgagggaag agttatttct tttttcggta aataacccga ttaacaacct
     3001 ggacgttgaa attcagatat ttgatgataa ctacagagag ggtgtaacag aaaagatcaa
     3061 attggatagt acggaaacct caaatgataa gataaaagag ttaacagcct ggtataaagt
     3121 aaatgctgat aatgtaccga tatacggcag tccatctgaa aaaagctcta tagttgcata
     3181 tgcatcaaaa gattcattat ttaaatcaga tatatcaaat aatgagtggg ctaaagtatc
     3241 tttagatagt agttcaaggg gatggataaa tatgaaattt ttgactacaa ctgataagaa
     3301 ggttaccgag gcaaatatta caattacctc ttcattccaa cagcctcctc aaataaagct
     3361 ggaccagcct ccgctgttaa tcaaaaataa tgagatatct ctaaaaggta tagtacaaga
     3421 tagtgacgga gtgaggctta taactgttta tcttggtgaa gataaagtat atttaaaaaa
     3481 cttttattcg caatctgcag ctgttgatac taagataagt cttaagagtg ggataaacta
     3541 tattaatatt tttgcaaagg ataaattagg gcttatttct aaaaaaactt atatagtaag
     3601 atatgatgcc tgaaactgtc ttatgtgatt ttattagtta atgattaaaa tattagcaag
     3661 ccgtcttttt acaggactat ttgttgttct aacagtaatt tcgattacgt tttttctatt
     3721 gagatccctt ccgggaggcc catttgatac agagaaaaag cttcctgccc aaatacaaat
     3781 aaatattgaa aagaaatata atctcgatca gccgctatgg aaacaatact ccatatacct
     3841 ttataatata tgcaggggtg atttcggtcc ttcttacaaa tatgtcgaca gatcggtaaa
     3901 tgatataatc aaacaaactt ttcccgtctc tttgaaactg ggaatcgtta gtcttatatt
     3961 atctctttta ctgggatgcg tagtgggaat attctcttct ataaaaagag gaagatcatt
     4021 tgactttctt tctttttcat tttcaacagc gcttatttct gtaccaagtt ttgttatagg
     4081 cgcatttctt atatacatat tctcaattaa atatgggatt cttccggctg ctttatgggg
     4141 tgagcctaaa cattatatac tgccgggcct tacccttgca gctggtcctg ctgcatatct
     4201 tatgagactt gtaagatcca gcatgcttga tgttacaggg gcgcagttta ttagaacagc
     4261 aaaggccaag ggactgaaaa catataaaat aatattcaag catatattta aaaacgctct
     4321 tattcccata gtaaccgtgc tggggccgat tactgcttac cttgtaacag gatcatttgt
     4381 agttgaacac atatatgcta ttcccggaat gggcaaattc tttgttttag ctgtttcaaa
     4441 cagggactat ccacttatta tgggtataac catagtctat acagtagtat tggtagtctc
     4501 caatttaata gttgatatgt tgtatttaaa gcttgatccg agaataagat ttggaatcag
     4561 cacagacaaa tgagaaatat aaagcttttt atagaatatg atgggactaa ttatcatggc
     4621 tggcagagac aaccaggcct caatacaata caaaagctta tagaagaaag tatacaaaaa
     4681 ataactaatg aaaaaacaat aattacagga tcaggaagaa cagacagcgg cgtgcatgca
     4741 atggagcaag tggccaactt taaaacttca tcaactataa atactgatag aattacaaaa
     4801 gctcttaaca gcgtacttcc ctgtgatatt gctattataa agtctcagca agtacatatt
     4861 gattttcatt ctcagtacga ttcattgaga aaaacctata tatacaaaat tctcaacagg
     4921 caaagcagga cagcaataaa cacaaagagg gtctggcata taccatataa actcgatcta
     4981 aaaaaaatgc agaacgctgc ggccagtctc ctgggcgaac atgatttcct ggtattttcc
     5041 aaaacaggct caggtgttaa gacaactgta agatcaatat acaaagcatc atttacaaaa
     5101 aaaagagacg ggacaattga gttttgtatc caggcaaatg gctttctaaa gggtatggtt
     5161 agattaatca caggagcact tgtaagtgtt ggcaaaggca aaatgactga gagtgagttt
     5221 gcagaaataa tctcaagcgg taaaaaacat aaatatataa aatccgcccc tgcgcacggt
     5281 ctttatcttg caaaagttgc gtatgacagt taagaatttt agctaaaact tatttaaact
     5341 ttaaaatcat gagaatatgc agatttataa acacaaattc acaaacatgc tatggaataa
     5401 tagaaaatac aacagtaact gagattgata acagtccatt tgaagattat tcaataacag
     5461 ataagtcata tgatctggac gagctaaagc ttctagcacc ctgcgtccct tccaaaatcg
     5521 tagcaatcgg actcaactat aaagatcatg cagcagagat gaacaagaaa atccctgaag
     5581 accctatgct ttttctaaaa ccaggctcca gtgtgatagg aaatgaagat aaaatcatct
     5641 atccggctca tatgtcaaag agagtagatt acgaaggaga gcttgcagta ataataaaaa
     5701 gagttgcgaa aaatgtaagc atggaaaatg ctatggattt tgttttgggt tatagctgtc
     5761 taaatgatgt aacggccaga gacctgcaaa gcaaggacat ccaatttaca agagccaagg
     5821 ggtttgatac ttttgctccc attggtccat ggattgaaac agatattgat ccaactaatc
     5881 tggatataaa aacattttta aatggcgaat taaaacaaaa ttccagtaca tccaatttaa
     5941 tttttaatat ccctaaatta ataaatttta tatcccatgt tatgactttg tatcccgggg
     6001 atgtcatatc cactggaact cccggaggca ttggacccat gaaaataggt gataaagtgg
     6061 gtattacaat acaaagtata ggtacactta ctaactatgt ggggtaacat ctattgatag
     6121 cgttcagtat aatttaaaat atattctgct ctttctcttt gctttttatc gagctttgct
     6181 tttccagatt tgagtgaacg atcagcaaat ttgtgtgcct gcttaaaatc acccagcctt
     6241 aaatacactt ctgctactaa taaatgaagt cttctattgt attttgaacg cttatctact
     6301 tctaatagaa cctgtaatgc ttttttgtac ttttc
//
LOCUS       DBNGSV010000211        11087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1907660, whole genome shotgun sequence.
ACCESSION   DBNGSV010000211 DBNGSV010000000
VERSION     DBNGSV010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1907660"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..2811
                     /locus_tag="AC159U_07650"
     CDS             13..2811
                     /locus_tag="AC159U_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336970.1"
                     /translation="MESFVYNILEFLTKPLLSFYGWVWGIVIPWVQQPSPWRQNRTEF
                     ISKFPEVVIMGTYVSAGSKAYYLDSYIANTIHGKPTDNVSVTYATTYSGYWKPVDVLV
                     LHETGSIVRHRQIMERLSKNGFLGPIVKFGQKYQLIQPGYPLSSRQSRGFKDINFSSS
                     GRLFVSRIDPETQNGEIYEIKPPESEPEIVGSILNCKAVKWLDIPSNISWQGYFSFSD
                     DDVLHISVNRPRESGEIRKTNAIVGQSVQSLSSISIYKEPNRSILGFDLNGPSQIYYT
                     SNVNLLTVADIKNNTQNDYMHFSSLNSSVMLDYSVRKLNFNKVVLTSSGVELDPFLRL
                     NPKIRSHMVWWFIDETKKDAEGEWLPYDKWPAHGKEKLFNAYNKIRNNQNPDFPEISK
                     DISEEGASNIYYAYTAFSLWVEINNKVNWSLLDLKGSEISNDDCLYMMLDGRHMFIDI
                     INNGNNEFIYGHHYNKAYYSPRKTDHKMDVFPAQPRFVFNFLAQNNLIRHSIRDTVKE
                     ILRWYEINLSHRSGCPWVNQPKEFFEKHGVENLSEFIKEKLGVNSVYETPIEWVLTKR
                     KYETAELDSKGEFYAWHGCHSAASLTYVFLHVLNVPSEVWSAIVSPGDCGKVDGTCTN
                     YGHEGIKIPTEDLISEHMDHFYANLHFLHDPTFDLFKIFMTRTQAKNFYKQMITDGDQ
                     DYDIEQALFRRLALLALQHPTALVVIRAWVAYNNATRLSMAAGRTISTEELTTAIYKE
                     LRRALGTSTEDKLYQETNIRPTRIPQDLFKELDKYKPADGRKDLPSDSLVRKFLNDFN
                     KAVYNTATIMSKVVKDENGKDTIAWIVATEDESTNTSHKYYLRKFSHIGQVWIKIHTV
                     QYVEYTYYRVNEPTSEPETHLAKDYISKKHAIKKSELLKISRDLVTHIQNNEQMGLKI
                     EDFEAKYDQWSGGKYSWG"
     gene            2984..3187
                     /locus_tag="AC159U_07655"
     CDS             2984..3187
                     /locus_tag="AC159U_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336971.1"
                     /translation="MYICSRCYYSGLPTGGAVEAKSGGLSGFINSLFGSGNKKLSCPN
                     CEKETFISLVSDEGKEILDSVTK"
     gene            complement(3199..4185)
                     /locus_tag="AC159U_07660"
     CDS             complement(3199..4185)
                     /locus_tag="AC159U_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3336972.1"
                     /translation="MNIFITGATGFIGRQLTKKLVENKFNVTALVRNKYKAKELTELG
                     VTIHEGDVTDVNSIEGAMRGCDTLFHLANISRWWLRDKSKYIDVNVTGTKNLLKEAKK
                     SGVKKVIFTSSVAAIRQPHGVLAKEDLEHCGEIESSYGKSKYLAEQEVLKFAKTNKMD
                     ISVLNPGVVIGPGDFKTFGKMLIEFLNRKLKFRAFDESYVPLVYIDDVVDAHIIAAEK
                     AKSGQKYILVSENLKIIDVYKMAHEITGDPIPSFQMPPFMIKLIAHIMEIKASVTGVS
                     PKLAVDAVNAMEKGASASNIKATDELGIKFMPIREALEKTINWYKENGYVNK"
     gene            4542..6023
                     /locus_tag="AC159U_07665"
     CDS             4542..6023
                     /locus_tag="AC159U_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3336973.1"
                     /translation="MKYFLITAFLISSSLFFSSCERVKENVVKEQPKQEVKTEKPKPV
                     ESADRNEIPISPSTGLPSFADLVKTLKPAVVNISTTNKVQRRSSPFSYPFDQGHPFDE
                     FFKKFFEGEPSQEFKQRGLGTGFIISKDGFIVTNNHVIEKADDIEINLEDGEKYEAKI
                     IGKDPKTDLALLKIDAERDFPFVEFGDSENSEIGDWVIAIGNPFGLGHTVTAGIISAK
                     GRILGIGNYDDFIQTDAPINPGNSGGPLFNLKGEVVGVNTAIIARGQGLGFSIPVNLT
                     KHVIDQIKDSGRVVRGWLGISIQKVTPEIADVIGVKDGEGVLVADVMDKSPAQTGGLL
                     RGDIILEYNGEKIVEVSDLTGKVALTPPGEISRLKIIRDKKIQEIRLKIGEFPEDEKV
                     ATKVDEPKAKFGLVVVDITPKIANRLNLDSTDGVIVNSVSRGSAAGEAGFKRGDIILE
                     INRVKVKNVDEYNKRLEDIGEGNSALFLIKRGETTVYIGLKIE"
     gene            complement(6034..6705)
                     /locus_tag="AC159U_07670"
     CDS             complement(6034..6705)
                     /locus_tag="AC159U_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR3336974.1"
                     /translation="MSSTIGQETSSVMEDYIKAIFNLIQKKGKANTNDLAKSLSVSAP
                     TVTQMVKRLVDLKLVTHEPYKGVALTESGNKMALETIRHHRLIEQYLYEALGVPWDKV
                     HNEAEKLEHVISEDLEKRMAEALNHPTHDPHGSPIPTKDLILESEDLCLLTDLEEGTK
                     GTISEVPDEDPKLLQYFDELGIKPDTKFTLIKIEPYGGSIVLEIDGNNVNVGSEAASQ
                     ISVKT"
     gene            6897..8177
                     /gene="egtB"
                     /locus_tag="AC159U_07675"
     CDS             6897..8177
                     /gene="egtB"
                     /locus_tag="AC159U_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR3336975.1"
                     /translation="MRTRNFEKDELFEQFRAVREFSEQISSPLCTEDYVIQSMPDVSP
                     TKWHLAHTSWFFEAFVLEKYDKKYKSLHPQYNYLFNSYYALVGERHCRPKRGLISRPT
                     VDEVYKYREYVNSKIEGLFTKSSEKEFQKHAPVIEIGVHHERQHQELMVTDIKHVFSE
                     NPLRPVYLEAPPNGNNGKITKHKWISFPEGLYEIGHQDESFCYDNETPRHKKFLNPFK
                     LSSRLVTNGEFIEFIEDGGYTRPELWLSNGIYTAEDNGWQAPLYWEKEGSKWHHFTLS
                     GFKELNLAEPVCHVSFYEADAYARWKGARLPTEAEWEITSEDLEIEGNFVEDARYNPV
                     PLNGGSKNGKLHQMYGDVWEWTSSPYVCYPGYKTLPGALGEYNGKFMSNQMVLRGGSC
                     ATSITHIRNTYRNFFPPDSRWQFMGIRLAQDENR"
     gene            8183..9145
                     /gene="egtD"
                     /locus_tag="AC159U_07680"
     CDS             8183..9145
                     /gene="egtD"
                     /locus_tag="AC159U_07680"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR3336976.1"
                     /translation="MSESVLKLNTEDPSDQHLLEEILVGLSKEQKELPCKLFYDKKGS
                     ELFDQITALPEYYPTRTEIGIIKSNIDEIAKCIGKNSLLVELGSGSSMKIRLLLGGLK
                     EISAYVPLDISYNHLIESTNTLSKDFPDIRIIPLCVDYTKPFEFPKFDFEWEKLVVFY
                     PGSTIGNFHPSYARKFLANVAKRTGKGSGLLIGVDLKKDKAVIENAYNDTKGITAEFN
                     LNILYRLNSDIGFNFDTDKWEHSAIYNENQGRIEMHLVSLTNQRVSHNGSKISFDKDE
                     SIITEYSYKYSLDEFEELIKDYYNVQKVWVDSNKRFSLQYLSVI"
     gene            9161..10147
                     /locus_tag="AC159U_07685"
     CDS             9161..10147
                     /locus_tag="AC159U_07685"
                     /inference="COORDINATES: protein motif:HMM:NF042530.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HIR3336977.1"
                     /translation="MGTEENIPESTEVSYILECCSELDSKGHKFVFLAEHVLDINRDE
                     AASLLAVDLNTIDTLINKKNEILQTCSYTTDLFKTQNEIINLADLFFELLNKINYSEA
                     INKNLKKSFIFKVIGLFKLFIESGYSNKQTIHAFISYLYFNLSRQDTIFSAERKLISL
                     REQDRSKWDVQLINKGIYHLEESADGNNVTIYHLESAVAAIHCLSKSYRQTDWNKIVS
                     VYDKYLSVNESPYVELQRASVISKIKGPRQGIEEIMNIGDMKSLEDSPLLYSTLGNLH
                     LQIHNYSTALENYEKAYAYAEIEVDKNFYHQKVQICRQRLKMIENYHFYNSF"
     gene            10223..10954
                     /locus_tag="AC159U_07690"
     CDS             10223..10954
                     /locus_tag="AC159U_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3336978.1"
                     /translation="MIELKNISKSYGSTIVIKDLNLHIDSGTTTVLIGQSGCGKSTTL
                     KLINGLVCPDKGSVIVGNNDITELDIFTLRKNIGYVIQAGGLFPHLTANENISIVAEY
                     LGMEKQEILDRTEQLCKLTKFPEDALSRYPVEISGGQRQRVSLMRALMLDPDVLLMDE
                     PLGALDPIIRYDLQNDLKEIFSTLNKTVVMVTHDINEAHFFADEMVLINEGRIVQQGT
                     IEQFTNNPADSFVTKFINAQRNTIS"
BASE COUNT         3680 a         1890 c         2142 g         3375 t
ORIGIN      
        1 agggggttaa aaatggaaag ttttgtctac aacattctag aatttttaac taaaccattg
       61 ttatcatttt atggctgggt ttggggtatt gttataccct gggtccaaca gccaagtcca
      121 tggagacaaa atagaactga gtttatttcg aaattcccag aagttgtaat aatggggaca
      181 tatgtgagtg cagggagtaa agcgtactac ctggattcct atatcgcaaa tacgattcat
      241 ggcaaaccca cagataatgt tagcgtaact tacgccacca cctatagtgg atactggaaa
      301 ccggtagatg ttcttgtact acacgagacc gggagcatag tgcgtcatag acaaataatg
      361 gaacgactta gtaaaaacgg ctttctgggc cctatcgtaa aattcgggca aaaataccag
      421 ctaatccagc ctggctatcc tctgagctcc cggcaaagca ggggatttaa agatataaat
      481 ttttcatcct ccgggcggct ttttgtaagc agaattgatc ccgaaaccca aaacggagaa
      541 atatatgaaa ttaagccgcc tgaatcagaa cctgaaatcg taggctcaat tctaaattgt
      601 aaagcagtta aatggcttga cataccatca aatatatcgt ggcagggata tttttcattt
      661 tctgatgatg atgtattgca tatctcggtc aacaggccgc gtgaaagtgg tgaaataaga
      721 aaaaccaatg ccatcgttgg gcaatctgtt caatcactat catcaatatc aatatataaa
      781 gaacccaata gatcgatcct gggctttgat cttaatggcc catcacagat ttactacacg
      841 agcaatgtaa accttctcac tgtggctgat attaagaata atacacagaa tgattacatg
      901 catttttcaa gtctaaattc ttcagtaatg ctggactatt cggtaagaaa acttaatttt
      961 aacaaagtag ttctgacttc ttcgggggtt gaacttgatc cattcttaag actaaaccct
     1021 aagatcagat cacatatggt ctggtggttc atagatgaga ctaaaaaaga tgctgaagga
     1081 gagtggctgc cttatgataa gtggcctgct catggaaaag agaaactgtt taatgcctat
     1141 aacaaaatta gaaataatca aaatcccgat tttccagaaa tctcaaagga tatttctgaa
     1201 gaaggggcta gtaatatata ttatgcctat actgcttttt cactttgggt tgaaatcaat
     1261 aacaaagtaa actggagttt actagaccta aagggatcgg aaatcagtaa tgatgattgt
     1321 ctgtatatga tgttggatgg acggcatatg tttatcgata ttattaataa tggaaacaac
     1381 gaatttatat atggccacca ttacaataaa gcatactata gcccaagaaa gactgaccat
     1441 aaaatggatg tattcccggc acaaccacgt tttgtattta attttctagc acaaaataat
     1501 ctgattagac atagtataag agatacggta aaagaaatat taagatggta tgaaataaat
     1561 ctcagtcata ggtcaggatg cccttgggtt aatcagccta aagaattttt tgaaaagcat
     1621 ggagttgaaa atctttcaga attcatcaaa gaaaagttag gtgttaacag cgtttacgaa
     1681 acacctatag aatgggtatt aaccaaaagg aaatatgaaa ctgctgaatt agattctaaa
     1741 ggtgagtttt atgcctggca tggttgtcat tcagctgctt ccctgaccta cgtattttta
     1801 catgttctta atgttccttc ggaggtgtgg tcagcaattg tttcacccgg cgattgcggt
     1861 aaagttgatg gaacttgtac aaactatggc cacgaaggaa taaaaatacc cactgaagat
     1921 cttatttctg aacatatgga tcatttctat gctaatcttc attttttaca cgaccccaca
     1981 tttgatttat tcaagatatt catgacgcgc acacaggcta aaaactttta caaacaaatg
     2041 attacggatg gagatcagga ctatgatata gagcaggcat tgttcaggcg gctggcactt
     2101 ttagcactac aacatcccac agcacttgta gtaatcaggg cttgggtagc ttataataac
     2161 gctaccaggt tgagtatggc tgcaggcaga accatttcaa ctgaagaact tacaactgca
     2221 atatataaag aacttagaag ggctctgggt acttctacag aagataaact ttatcaagaa
     2281 acaaatattc ggcctacacg aattccgcag gatcttttca aagaattaga caagtataaa
     2341 ccagctgacg gaagaaaaga tctgccgtca gattctttag tacgcaaatt cctcaatgat
     2401 tttaataagg ctgtctataa taccgcgact attatgtcaa aagtagtcaa ggatgaaaat
     2461 ggcaaagata cgattgcatg gatagttgcc acagaagatg aatctacaaa tactagtcat
     2521 aaatattatt taagaaaatt ttctcatatc ggacaagtgt ggattaaaat tcatacagta
     2581 cagtacgtag aatatacata ttatcgcgtc aatgaaccaa cgagtgaacc tgagactcat
     2641 ctagcaaaag actatatctc caaaaaacat gcaatcaaaa aatctgagct tttaaaaata
     2701 tctcgggatc tggtaacaca tatacagaat aatgaacaga tgggtcttaa gatcgaagat
     2761 tttgaggcta agtatgatca gtggtcgggc ggcaaatata gctggggata agacttagac
     2821 aatcctttac gtataaagac cataagattg ttggataagt tatatgagta actaatataa
     2881 gcttatctcg gattaaattc ctacaattga attaaaaaaa tcttctccaa ataaaaatgg
     2941 atgtataatt tgctttatat gttcattttt aaggagaggg gaaatgtata tctgttctag
     3001 atgctactac tccgggcttc cgacaggtgg agcggttgaa gctaaatctg gtggactatc
     3061 aggatttatt aattcactgt ttggttccgg aaataaaaaa ttatcttgtc ctaattgtga
     3121 gaaagaaact ttcatatctc tagtttctga tgagggaaaa gaaattctgg attctgttac
     3181 caaataaccc tgattaattt acttgtttac ataaccattt tccttgtacc aattaatagt
     3241 cttttctaaa gcctctctga tcggcatgaa ttttattccc agttcgtcag tagcttttat
     3301 gttggatgcg cttgcacctt tttccatggc atttacagcg tctactgcaa gtttgggaga
     3361 tactcctgtt acagaagctt ttatttccat tatatgagca atgagcttta tcataaaagg
     3421 aggcatttga aatgagggaa taggatcgcc agtgatttca tgagccattt tatatacatc
     3481 aatgattttc aggttttcac ttacaagaat atatttctga ccggattttg ctttttctgc
     3541 agcaataata tgggcatcaa ctacatcatc gatataaaca agaggtacat aactttcatc
     3601 aaatgcccgg aacttgagtt ttctattgag aaattctatt agcatttttc caaaagtttt
     3661 aaaatccccc gggccaataa caactccggg atttagaaca gaaatgtcca tcttattggt
     3721 ttttgcaaat tttagaactt cctgctcagc aagatatttc gacttaccat aagagctttc
     3781 aatctctcca cagtgttcca gatcttcctt agctaatacc ccatgaggct gtcttatagc
     3841 ggcaacagag cttgtgaata ttactttttt tactcctgat tttttggctt ctttaagcag
     3901 attttttgtt ccagttacat ttacatcgat atatttgctc ttatccctta accaccatct
     3961 ggatatattt gctagatgaa aaagagtatc acagcctctc atcgcaccct ctattgaatt
     4021 tacatctgta acatctcctt catgtattgt tactcccagc tcggttaatt cttttgcctt
     4081 gtatttattt cttacaagtg ctgtaacatt aaacttattt tcgaccagtt tcttagtaag
     4141 ctgcctgcct ataaatcccg tggctcccgt gataaatata ttcatataat taaatgattt
     4201 gattaataag gtttaatttc taagttggat gcattattat tgtgatcgat acacataaat
     4261 caataaacgc tttgcaaatt agtgagagat aattatgaaa cagattttaa attcgaaacc
     4321 tttttggata aatctggtaa tattttaata aaaatggtaa taacacgatt gacatttaag
     4381 taatatactt taatttaaat tacaaaatta atataataac ttaaattatg tttgagattc
     4441 gaataataag acttttattt ataccttttg tctgttagct tttcaccttt actaattact
     4501 aaaatgaaaa tagtatctat tactacgatt cggaggaata tatgaaatat tttttaatta
     4561 ctgccttttt aatatctagt tcattatttt ttagctcatg cgagcgtgta aaagaaaatg
     4621 ttgtaaaaga acagcccaaa caagaagtta aaactgaaaa accaaaacct gtagagagtg
     4681 cagatagaaa tgaaattccg atctcacctt caaccggcct accatcattt gctgatcttg
     4741 taaaaacatt aaagcctgca gttgtgaata taagcacgac caacaaagtg cagagaagat
     4801 catctccatt ctcttatccc tttgatcagg gccatccttt tgatgaattt ttcaaaaaat
     4861 tctttgaggg agagccttca caggaattta agcaaagggg tcttggtacg ggattcataa
     4921 ttagtaaaga cggctttatt gttacaaaca accatgtgat tgaaaaggca gacgatattg
     4981 agattaattt agaagatgga gagaaatacg aggccaagat tatagggaaa gatccgaaaa
     5041 ccgatttagc actgctaaaa atagatgctg aaagagattt tccatttgta gagtttggtg
     5101 attctgaaaa ttctgaaata ggagactggg taatagcaat tggaaacccg tttggccttg
     5161 gacataccgt aactgcagga ataataagtg ccaagggaag aattcttggt attggaaact
     5221 atgacgattt tattcagacc gacgctccaa ttaacccggg aaacagcggc ggacctttat
     5281 ttaaccttaa aggggaagta gtaggggtaa acactgcaat aattgcaaga ggtcaggggc
     5341 ttggtttttc aattcctgta aaccttacta agcacgttat agaccagatc aaggacagtg
     5401 gcagagtggt aaggggatgg cttggaattt ccatacaaaa agttacaccg gaaattgctg
     5461 atgtaattgg cgtaaaagat ggtgaagggg tattagttgc agatgttatg gataaaagtc
     5521 ctgctcagac aggagggctg ctaagaggtg atatcatcct tgagtataac ggtgaaaaaa
     5581 tagtagaagt atcagattta acaggaaaag tagcgcttac tccgccagga gagatatcta
     5641 ggcttaagat aataagggat aaaaaaatcc aggaaataag actgaaaatt ggcgaatttc
     5701 cagaggacga aaaagtagca acgaaagttg atgaacctaa agcaaagttt ggtcttgtgg
     5761 ttgtagatat tacaccgaaa attgcaaaca gattaaatct cgattctact gatggggtaa
     5821 tagtaaattc tgtttcaaga ggttctgctg cgggcgaggc aggttttaaa agaggggata
     5881 taatattaga gatcaacagg gtaaaagtta aaaatgttga tgagtacaat aagcgccttg
     5941 aagatatagg cgaaggtaat tcagcgctct ttttgattaa aaggggtgaa actacggttt
     6001 atattggtct taagatcgaa taatgtgcta cttttaggtc ttaacactta tttgagaagc
     6061 agcttcagat cctacattta cattatttcc atcaatttca agaactattg agccaccata
     6121 aggctctatt tttatgagtg tgaatttagt gtcgggcttt attcccagtt catcaaaata
     6181 ttgtagaagc ttagggtctt catccggaac ctcagagata gttcctttag taccctcttc
     6241 aagatcggta agtaaacaaa ggtcttcact ctctaaaata aggtcttttg tgggaatggg
     6301 agacccatgt ggatcatggg taggatggtt taaggcttct gccattctct tttccagatc
     6361 ttcggatatt acatgttcta gtttttcagc ctcattatgt accttatccc atggcactcc
     6421 caaggcttca tacaggtatt gttcaattaa tctgtggtgt cttatggttt ccagagccat
     6481 tttatttcct gactctgtaa gagctactcc cttataaggt tcgtgagtta caagttttag
     6541 gtctacaagc cgttttacca tttgtgtcac agttggtgca gacacactca aactcttagc
     6601 aagatcgttg gtgtttgcct tgcctttttt ttgtatcagg ttgaaaatag cctttatata
     6661 atcttccatt acagaagaag tttcttgtcc tatcgtgctg ctcattatgt actataaaca
     6721 ataatatctg ttagttccaa atatttttat acgcctttaa taccattata agtttgaaat
     6781 cataacctag tataatatac tattacatta aatcattgtt tttacttttt ataagaatct
     6841 aatttctaaa ctttttatct aaataaatca ggttttaaca aatcgggaca caactaatga
     6901 gaacgcgcaa ttttgaaaaa gatgaactat ttgagcagtt tagagctgta agagagttta
     6961 gtgaacaaat ttcatctccg ctttgtacag aagattatgt cattcagtcc atgcccgatg
     7021 ttagccctac aaagtggcac cttgctcaca caagctggtt ttttgaagcg tttgttttag
     7081 aaaaatatga taaaaaatac aagtcactcc atcctcagta taactacctg tttaattcct
     7141 attatgcttt agtgggtgag agacactgta gacccaaaag agggcttatc tcgagaccta
     7201 cagtggatga agtgtataaa tatagagaat atgtgaacag taaaatcgaa ggacttttta
     7261 caaaaagcag tgaaaaagag tttcaaaagc atgctcctgt tattgaaata ggcgttcatc
     7321 atgaaaggca gcatcaggag cttatggtta ctgatattaa acatgtattt tcagaaaatc
     7381 cattaagacc ggtttattta gaagctccgc ctaatggaaa taatggcaaa ataaccaaac
     7441 ataaatggat ttcgttccca gagggcttat atgaaattgg ccatcaggat gaaagttttt
     7501 gttatgataa tgaaacaccg agacacaaga aatttctaaa tccgttcaag ctctcatcca
     7561 ggcttgtaac aaacggggaa tttattgaat ttatagagga tggtggttat acaagaccgg
     7621 agctttggct atcaaatggt atttatactg cagaagataa cggatggcag gcaccgctgt
     7681 attgggaaaa agaaggaagt aaatggcatc actttacact ttccgggttt aaagagctaa
     7741 acctggctga gccggtatgt cacgtgagct tctatgaggc tgatgcctat gcccgctgga
     7801 aaggtgcaag gcttcccact gaggctgaat gggaaataac ttctgaagac ttggaaattg
     7861 aaggaaactt tgttgaagac gccagatata atcccgtacc tcttaacggc ggctcaaaaa
     7921 acggaaagct tcaccagatg tatggtgatg tgtgggaatg gacatcaagc ccttatgttt
     7981 gttatccggg gtacaaaact cttccgggtg ccctgggtga atataacggg aagtttatgt
     8041 ctaaccagat ggttctgcgt ggcggctcat gcgcgacttc tatcacacat attagaaata
     8101 cttacagaaa tttctttcca cctgactcaa gatggcagtt tatgggtata agactcgcac
     8161 aagatgaaaa tagatgatac taatgagtga aagtgttctt aaattaaata ccgaagatcc
     8221 cagtgaccag catttattag aagaaatact agttggtctc agcaaggaac aaaaggagct
     8281 tccatgcaag ctgttttatg acaaaaaggg ctcagagctt ttcgatcaga taacagctct
     8341 tccggagtac tatcccactc gcacagaaat tggaataatt aagtccaaca tagacgagat
     8401 tgccaaatgt ataggtaaaa acagccttct tgttgagctt ggaagtggaa gtagtatgaa
     8461 aatacgcctt ctcctaggag gattaaaaga gattagcgca tatgtgccgc ttgatatttc
     8521 ctataaccat cttattgaat ctaccaatac gctttcaaaa gattttcccg atataagaat
     8581 aattcctctt tgtgtcgact acacaaagcc atttgaattt cctaaatttg atttcgaatg
     8641 ggaaaaattg gtagtctttt accccggctc tacaattggt aattttcacc cctcttatgc
     8701 aaggaagttt ctggcaaatg ttgcaaaacg cactggtaag ggaagcgggc ttctaatagg
     8761 ggttgatctt aaaaaagaca aggctgttat tgaaaatgct tataatgaca ctaaaggaat
     8821 aacggcagag tttaatttga atattctgta caggctaaac tctgatattg gttttaattt
     8881 tgataccgat aaatgggaac atagtgcaat atataacgaa aatcaaggca gaatagaaat
     8941 gcaccttgta agtttaacta atcaaagagt atctcataac ggcagcaaaa tatcttttga
     9001 taaggacgaa tcgattataa ccgagtattc atataagtat tctctggacg agtttgaaga
     9061 acttataaaa gattactata atgttcaaaa agtctgggta gacagcaaca aaagatttag
     9121 cctgcagtat ttaagcgtta tatgagtatt aattaaaaca atgggaacag aagaaaatat
     9181 tccggagagt accgaagtat cttacatact agaatgctgt agtgaattag attctaaagg
     9241 gcataaattt gtgtttttag ctgaacatgt gcttgatatt aatagagatg aagcggctag
     9301 tctgctggca gtagatctca atacaattga tactcttatt aataaaaaga atgaaatttt
     9361 acaaacctgt tcatacacta cagatttatt taaaacacag aatgaaatta taaaccttgc
     9421 tgatttgttc tttgagcttc taaataaaat taactactca gaagccataa ataagaatct
     9481 taaaaagagt tttatattta aagtaatagg cctgtttaaa ctttttattg agtcaggtta
     9541 ctctaataaa caaacaatac atgcgtttat ttcgtatctt tattttaact tgtccagaca
     9601 agacaccatt ttttctgctg aaagaaagct catatctctt agggaacaag acagatcaaa
     9661 gtgggatgtg caattaataa acaaagggat ttatcatctg gaagaatcag cagatggaaa
     9721 caacgttacg atctatcatc tggagtcggc agttgctgca atacactgtc tttctaaatc
     9781 ttacagacaa acggactgga ataagatcgt atcagtctat gataaatatc tctcggttaa
     9841 tgaatcccct tatgtagagc ttcaaagggc ctcggtcatt tcgaaaatta aggggccaag
     9901 gcagggaatc gaagagatta tgaatatagg tgatatgaaa agtttagagg atagtcccct
     9961 tttatattca acccttggaa acctgcacct acagatacat aactactcaa cggctcttga
    10021 gaattatgaa aaagcctatg cttatgcaga aattgaggta gataagaatt tttatcacca
    10081 aaaagtacag atatgcaggc agcgcctaaa aatgatagaa aattatcatt tttataactc
    10141 tttttgatct ttttctactc tcaccacagt taatttataa gcttctctgt ttaatttctt
    10201 ataaacaatt agaataatat ctgtgataga gcttaaaaat atttccaaat cctatggcag
    10261 tactattgta ataaaagact taaatctaca cattgattct ggcacaacta ctgtgctaat
    10321 cgggcaaagt gggtgtggta agtctactac tctaaaactt attaacggac ttgtttgccc
    10381 tgataaaggc agcgttattg taggtaacaa tgatataact gagcttgata tcttcacact
    10441 aaggaaaaac attggctatg tgattcaggc cggcgggctt tttcctcacc tgacagcaaa
    10501 tgaaaatata tccatagttg cagagtatct tggcatggag aagcaggaga tattggatag
    10561 aacagagcag ctctgtaagc tcactaaatt tcccgaagat gcgcttagca gatatcctgt
    10621 agaaatatca gggggacaga ggcaaagagt aagtctcatg agagcattaa tgttagaccc
    10681 cgatgtcctg ctaatggacg agccgctagg ggcgcttgat cctatcattc gttatgatct
    10741 tcaaaacgat ctaaaagaga tttttagcac actcaataaa acagttgtga tggttaccca
    10801 tgatatcaat gaagcacact tttttgccga tgagatggtt ttaattaatg aaggaagaat
    10861 tgttcagcag ggaacaatag agcagtttac taataatcct gcagatagtt ttgttactaa
    10921 attcataaat gctcaaagga atactattag ctaatgaaat ccatatatac cttatttagc
    10981 gtattattta ttatcctttt ttttacatcc tctcttcagt ctaaagagat tgtagttggc
    11041 tctaaaactt ttacagagtc tgtaatcctt ggagaaataa taaaaca
//
LOCUS       DBNGSV010000212         8840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_314112, whole genome shotgun sequence.
ACCESSION   DBNGSV010000212 DBNGSV010000000
VERSION     DBNGSV010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_314112"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="AC159U_07695"
     CDS             complement(<1..1214)
                     /locus_tag="AC159U_07695"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR3336979.1"
                     /translation="MSSRRVLLHGTNAIIFITVVIGILIFANYFALKNGGRMDLTKDK
                     LFSISDQTRQILTTVESEVEIIGFFKEVGLDRKEFLTLANQYKEYSDKVKLTMVDPDK
                     SPGLATQYGITEYSTVVIARDEDSLKINLTDPLSDGIINTSENEITNAIIKLAKESKK
                     TLYFIIGHGERDTADVSDVSGFGQLVNAVRDEGYEVSELLVKQNLDLPKYDSILVVAG
                     PRKTLLQTEIDAIKNFIDEGGNTIFMLEPQGSPELVKMLSGYGIKIGNNIVIDPSSKL
                     VGGGDVAPIVAQYPQHKITSDFKFATLFPFVRTVEPVSSEASNIESIAATSEYSWAET
                     NLDLFEQGKADFESGDRKGPVSVAVVGQLSEGGKIAVFGSAEFSSNRFLLFSGNKDYV
                     LNVVNWISGDENL"
     gene            complement(1211..1963)
                     /locus_tag="AC159U_07700"
     CDS             complement(1211..1963)
                     /locus_tag="AC159U_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3336980.1"
                     /translation="MKKTYTILKRELKSYFVSPIAYIVLLVFTVLTGVFFFIYLQGFA
                     QAQFDTRAQMFQQTFSLTEFVLQPYFGTISVLLLLIIPMLTMRLLSEEKKQHTSELLF
                     TSPIKVIHIVLGKYLAALSLFLLMLVFSAIFIVVLYIKGNPDPGVILTGFLGLFLLGA
                     SFLSVGLFASSLSQNQIVCAVISFGVLLLFWIVGSTSDAESSVVGYLSIINHLDNFIK
                     GVIEVKDIVYYISFIFFGLFLTHVILDSERWR"
     gene            complement(1960..2883)
                     /locus_tag="AC159U_07705"
     CDS             complement(1960..2883)
                     /locus_tag="AC159U_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3336981.1"
                     /translation="MIKVENLTKTYGSITAIDDISFQVNKGEILGFLGPNGAGKTTTM
                     RMITGYMPANEGRVEVSGFDVLDYPIKAKNEIGYLPENPPLYDDMKVADFLDFVAGLK
                     GVEEDKAQKIDSAMSRCGIADVSARLIGNLSKGYRQRIGIAQAILNDPSVLILDEPTI
                     GLDPKQIIEIRELIKQLGTDRTVILSTHILPEVMMTCSKVVIINKGKIALETSLVELN
                     KKLDEDNEVHIKVSNYSSEIKTTLNSLQSVQSTEDKGGGEFIVKSKSSNNTDEIARAI
                     IDNNWGLKEIRPRSNTLEDVFLNVIASDQKQ"
     gene            3168..5987
                     /locus_tag="AC159U_07710"
     CDS             3168..5987
                     /locus_tag="AC159U_07710"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3336982.1"
                     /translation="MNIWKEFHGPNSGYILELYERYSENPDSVDPSTRALFDLWKPET
                     EEQPQYIENKDMPDVQKIKGAVRLARSIRAYGHLAADIDPLGSKRHGDPSLNYQTHGI
                     TEHDLANLPASLIGGPIAVKCENAQEAIKELTKVYISTTGYDYDHIHTLEEREWLRGC
                     SESGKYNVVSIPIDPLALLNRLTEIDVFEKFLHRIFPGKFRFSIEGVDMLVPMLNEFI
                     DEASKSGIKNVLLGMAHRGRLNVLAHVLNKPYYHTFSVFKDPVHERDFRDDIGWRGDV
                     KYHAGFTKAVNGNGREIDMMISLAPNPSHLEAVNPVVEGMARAAGTISNQKGAPEFDP
                     SASIPVLVHGDSAFQGQGIVPETLNLSRLPGYSTGGTLHIITNNQLGYTTTPLDSRST
                     LYASDVAKGYELPIIHVNADDPEACIHGVRLGFTYIRKFQKDFLIDLVGYRKHGHNEA
                     DEPSFTQPKMYKKIENHPSVRELWAKKLVERNIIDEGHAEDLFNKQMEKLHSIFESLE
                     VDAAIKELQQKLPPPSRSGEINTSVKKKKLIEYHKELGKYPENFNLHAKLKRPLQKRI
                     EALDDHKEKSIDWALAEDLAFASILAEGTPIRFTGQDTQRGTFSHRHSVFHDYETGKR
                     FTPLQSFKQAKASFEIKNSPLTENACIGFEYGYNIQKPECLVIWEAQYGDFINGAQTM
                     IDEFIVSARAKWGLTPSLVLMLPHGYEGQGPDHCSGRLERFLGLAADANIRIANCSSS
                     GQFFHLLRRQALLLIEDPLPLVIMTPKSLLRNPDSFSSLNDLTEGTWQNVIDDPRVKN
                     KSKVKRLVFCSGKIYIDLDSSELRAKSPDIAIVRIEQLYPFPKMETKKIIETYKNIKE
                     IVWVQEEPSNMGSVMYLWPRFKQVFDDSKIAMHYIGRKPNSSPSEGSSSWHKVIQEAI
                     IKETFNLKSKKDTKGILWYSLETVN"
     gene            6006..7277
                     /gene="odhB"
                     /locus_tag="AC159U_07715"
     CDS             6006..7277
                     /gene="odhB"
                     /locus_tag="AC159U_07715"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR3336983.1"
                     /translation="MSKNIVVPELGESVVEATVIRWIKNEGEAVSMGETLVELETEKA
                     NFEVAAEAAGVLEKIVKNADEDVVIGDVLGTINENGTASAEVSTEPVQTETAAEPEPE
                     QAQPKPAEQIDVDVKVTPVAKKIIEQSGLNAYDISGTGPNGKITKEDVEDHIKSVSTA
                     ASEKPRAPEPAAKPAPSAPSTSVVIPEPESDGREERVRMSARRRTIARRMLEAQADAA
                     ILTTFNEVDMGPVMEVRKRRKDSFAEKHGVKLGFSSFFVKAVIGALKDFPAVNAEIQD
                     DYIVYKHYYDIGMAIGAEGGLVVPVLRDCDRMTFADIERTIIEYVGKANNNTLSLQEL
                     RGGTFTITNGGVFGSLLSTPILNPPQVGILGLHKIEERPVAVNGEVVIRPMMYTALSY
                     DHRIVDGKEAVQFLVKIKELIEDPEVLLLEG"
     gene            complement(7293..8684)
                     /locus_tag="AC159U_07720"
     CDS             complement(7293..8684)
                     /locus_tag="AC159U_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HIR3336984.1"
                     /translation="MSIPRDTNEQFENAVLGSILIEPKIMDEVSVALRPDDFYKSTNK
                     KLFEVLEDMHKNSAPIDLMTVFEYAKQKKADKNIGGPQRLIYLTETTPVSANFAYYVK
                     KVKDASERRSFVDTCSCISKELEQGKISVPEAWGLIAEMESSVTMRSGLKFTAASDIT
                     LNTESVQSLWGEVIYPQCITQINSEPGVGKTTFVYNLCIDGVKGSRFLDIPFSKSIKV
                     LYIDVETPGWKRGVKLNSISPDEVPAGLYFLDSLDMHTQFSELLCLCKNMGFDLIVFD
                     TQSRIFNLESENDNAEANRMMHLVRRLVSEAGAAVILVHHCSKTDKKGVYSGRGASAI
                     GGAVDVVVNLESLDGQSLKLKVDKNRISGDYMTLFIKKSGDDRFEPYIPPTESVSGFE
                     KFKVQEQILSLSDRKPQWTTVEIQSECQLKGFTESTIKRAISSLTLSGKITKTRRGEY
                     EITGTVQDTQSVH"
BASE COUNT         2601 a         1955 c         1890 g         2394 t
ORIGIN      
        1 agattttcat ccccggatat ccagtttact acgtttaata cataatcttt attgcctgaa
       61 aaaagcaaaa acctgttcga agaaaactct gcacttccaa atactgcaat cttaccgccc
      121 tcagagagct gccccactac cgctacagac actggcccct tcctgtcacc tgattcaaaa
      181 tcagccttac cctgctcaaa cagatcaagg ttggtctcgg cccagctgta ttcacttgtt
      241 gctgctatgg actcgatatt ggaagcttct gacgataccg gctctacggt tcttacaaaa
      301 ggaaatagcg ttgcaaattt aaaatcgctt gtgattttgt gctgagggta ctgtgctact
      361 atgggggcca catcacctcc tcccacaagc ttcgatgaag ggtctattac aatattattt
      421 ccgattttta tgccgtagcc cgaaagcatc ttaacaagct caggcgagcc ctggggctca
      481 agcataaata tagtgttgcc gccttcatcg ataaagtttt ttatagcatc tatctcggtt
      541 tgtaatagag tcttcctggg acctgctaca accagaatag aatcatactt tggcagatca
      601 agattttgtt taacaagaag ttcagatact tcatacccct catccctcac agcgtttaca
      661 agctgtccaa accctgatac atcagacaca tctgcagtat ccctttctcc atgacctata
      721 ataaaataga gcgtcttctt actttccttt gcaagcttaa ttattgcatt tgttatctcg
      781 ttttccgagg tatttataat accgtctgag agcggatcgg taaggtttat cttaagacta
      841 tcttcgtccc ttgcgataac caccgtagag tattctgtga ttccatactg tgttgcaagc
      901 ccggggcttt tatcagggtc taccattgta agcttaactt tatcggagta ctctttatat
      961 tgatttgcaa gggttaagaa ctcctttcta tccagcccta cttccttaaa aaaacctatt
     1021 atctctactt cactctcaac agtggtaaga atttgcctgg tctgatcgga gatggaaaag
     1081 agcttgtctt tggtcaaatc catcctgcca ccgtttttaa gagcaaaata gtttgcaaat
     1141 atcaatattc caataacaac ggttataaat attatagcgt ttgttccgtg aagcagcacc
     1201 cttcttgagc tcatcgccac ctctccgaat ccagtattac atgggtcaga aaaagcccga
     1261 agaatataaa agagatatag tagacaatgt cttttacctc tattacccct tttataaaat
     1321 tatccaggtg attaataatc gaaagatatc caacaaccga actctctgca tcagaagtag
     1381 agcctacgat ccagaataac agtaaaaccc caaaggatat gacggcacaa acaatctggt
     1441 tttggctaag agaagaagca aataacccaa cggagagaaa actggcgccg agcaaaaaca
     1501 ggcccagaaa ccctgtgagt attactccgg gatcggggtt gccttttatg taaagcacca
     1561 ctataaaaat tgccgagaac acaagcatca gcaaaaacag gctaagagcc gcaaggtact
     1621 tgccaagaac tatgtggatc acctttattg gagaagtaaa aaggagctcg gatgtatgct
     1681 gctttttctc ttcagaaaga agcctcatgg taagcatggg aataattagc agcaaaagca
     1741 cgctaattgt tccaaaatag ggctgcagga caaactcagt aaggctgaaa gtctgctgga
     1801 acatctgtgc gcgcgtatca aactgcgcct gggcaaaacc ctgcaggtat ataaaaaaga
     1861 atactcctgt tagaacggtg aaaaccaaaa gcactatata tgcaatcggt gatacaaaat
     1921 aggatttaag ctctcttttt agtattgtgt atgtcttttt cactgttttt ggtctgatgc
     1981 tattacgttt aggaacacat cttcgagggt atttgacctt ggcctgatct ccttaagccc
     2041 ccagttatta tcaataatag cccttgcaat ttcgtctgta ttatttgaac tttttgattt
     2101 aacaataaac tctccaccgc ccttatcctc ggtactttgt actgactgca gggagtttag
     2161 cgtggttttt atctcacttg aatagtttga tactttgata tggacttcat tatcctcgtc
     2221 taactttttg ttcaactcca ctaaagaggt ttccagagca attttgccct tattgatgat
     2281 cacgacctta gagcacgtca tcattacctc tggcagaata tgagtgctca ggatcaccgt
     2341 tctgtcagtg ccaagctgtt ttataagctc ccttatctca attatctgct ttggatcaag
     2401 gccgattgta ggctcgtcca aaataagcac agaggggtcg tttagtattg cctgagctat
     2461 tccaatcctc tgcctgtaac ccttagacag gtttccaatg agccttgcgc ttacatcagc
     2521 tatgccgcat ctgctcattg cgctgtctat tttttgagcc ttatcctcct ccactccttt
     2581 cagcccggca acgaagtcca aaaagtctgc aaccttcata tcatcataaa gcggaggatt
     2641 ctcaggaagg tatccgatct cgttcttggc ctttatagga tagtccagca catcaaaccc
     2701 cgacacttcc accctgccct catttgccgg catgtagccc gtgatcatgc gcatggtagt
     2761 ggtctttccg gctccatttg ggcccaggaa tcccagtatc tctcccttat taacctggaa
     2821 agatatatcg tctatggcag ttatgctgcc gtatgtcttt gtaaggttct ctacttttat
     2881 catcccaaaa atagagtcaa tagatatgaa atttcataaa aaattaatta ttcagataaa
     2941 attgtcaaga attatatttt gaaataattt ttgattgtta atatactacc ttaaatatgc
     3001 gggaaaaata attttattta aagaaaaatt gtattaaaat ataaattaac atataaaata
     3061 tgatatttat ttgaaaattg agatattaca tatttcacaa taaattttat atgatataat
     3121 tcgagccaaa tttcatattc catataaatg attcctatag gagtaaaatg aatatttgga
     3181 aagaatttca cgggccaaac tctggttaca tcctcgagct gtacgaaaga tacagcgaaa
     3241 atcctgattc agtcgatcca tccacaagag cgctgtttga tctatggaaa ccagaaactg
     3301 aagagcagcc ccagtacatt gaaaataagg acatgccaga tgtccaaaag ataaaaggag
     3361 ccgtaaggct tgcaagaagc ataagggcct acggacatct tgcagcagat attgatcccc
     3421 ttggttcaaa aaggcatggt gatccgtctc ttaactatca aacccacggg atcaccgaac
     3481 atgatcttgc caatctgcct gcaagtttga ttggcggtcc aatagcagtg aaatgcgaaa
     3541 atgctcagga ggcgattaaa gagcttacaa aggtttatat atccacaacc ggatatgatt
     3601 atgatcatat tcatacatta gaggaaaggg aatggctcag agggtgcagc gaatctggga
     3661 aatacaatgt agtgagcata ccgattgatc cgctagcgct acttaacaga ctcacggaaa
     3721 ttgatgtgtt tgagaaattc ctgcacagga tattccccgg caagttcagg ttttcaattg
     3781 aaggcgtgga catgcttgta ccaatgctta atgaatttat tgacgaagca tcaaagagcg
     3841 gtataaaaaa tgtgctttta ggaatggcgc acaggggaag gctcaatgtg cttgcccatg
     3901 tgcttaacaa gccctattat catacatttt cggtatttaa agacccggta cacgaaaggg
     3961 atttcagaga cgatattggc tggagaggag acgttaaata ccacgccggt ttcacaaagg
     4021 ctgtaaacgg aaacggcagg gaaatagaca tgatgatatc tcttgctcca aatccaagcc
     4081 accttgaagc tgtaaaccct gtagtggagg gtatggcaag ggcagcggga actatttcca
     4141 atcagaaagg tgcaccggaa tttgacccat cagcctcgat accagtactt gtacatggcg
     4201 actcggcatt tcagggacag gggattgttc ccgaaactct aaacctttcg agacttcccg
     4261 gatacagcac aggcggcact cttcatatta ttactaataa ccagctcggt tatacaacca
     4321 cgcccctgga cagcaggagc accctttatg caagcgatgt ggcaaagggt tatgaattac
     4381 caataattca tgtaaatgcg gacgatccgg aggcctgtat tcatggcgtt aggcttgggt
     4441 ttacatatat caggaagttt caaaaggatt tcctaatcga ccttgtcggt tacagaaaac
     4501 acgggcacaa cgaggccgac gagccttctt ttacccagcc caagatgtat aagaaaattg
     4561 aaaatcatcc ttctgtacgc gagttatggg caaagaaact tgtagaaaga aatattatag
     4621 atgaaggtca tgcggaggat ctgtttaaca agcagatgga aaaactgcac agcattttcg
     4681 aatcactgga ggttgacgcc gcaatcaagg aacttcagca gaagcttccg cctccgagca
     4741 gatcgggaga aatcaataca tcggtcaaaa agaaaaagct catcgaatac cacaaagaac
     4801 ttgggaagta tcctgagaac tttaaccttc atgcaaaact aaagaggcct ttgcaaaaga
     4861 ggatagaagc tcttgatgac cataaagaga aatccataga ctgggcgctg gctgaagacc
     4921 tggcatttgc atcgatactt gcagagggta cacccataag gtttacagga caggacaccc
     4981 agcgcggcac gttcagccac cgccattcag tgtttcatga ttatgaaaca ggaaaaagat
     5041 ttacaccgct gcagtcattt aagcaggcaa aagcctcttt tgaaataaag aactcaccgc
     5101 ttacagaaaa cgcctgtata gggtttgaat acggatacaa cattcaaaaa cccgagtgtc
     5161 ttgtaatctg ggaggcgcag tatggagact ttataaacgg cgctcagacc atgatagatg
     5221 agtttattgt gtctgcaagg gcaaaatggg ggcttacccc ttcgcttgtt ctcatgctgc
     5281 cgcacggata cgaaggccag ggccccgatc actgcagcgg aaggcttgag agatttttag
     5341 gtcttgctgc cgatgctaat atcaggattg caaactgctc aagctcgggt cagttctttc
     5401 atctgctccg gcgccaggcg ctgctgctca ttgaagaccc tctgccgctt gtgattatga
     5461 cgcccaagag tcttcttaga aatccagact cgtttagctc tctcaatgac cttacggagg
     5521 gaacatggca gaatgtgatt gacgatccaa gggttaagaa taaatcaaaa gtaaaacggc
     5581 tggtattttg cagcggaaag atttatattg accttgactc cagcgagctt agagccaaaa
     5641 gccctgatat cgcaatagtc agaatcgaac agctgtatcc tttccctaaa atggaaacaa
     5701 agaaaattat tgaaacctat aaaaatatta aagagatagt atgggtacag gaagaaccat
     5761 ccaatatggg gtcagtcatg tatctgtggc caagattcaa acaggttttc gacgattcaa
     5821 aaatcgcaat gcattatatc ggaagaaagc caaactcaag cccttcggag ggttcgtcat
     5881 cgtggcacaa ggtaatacag gaagctatta ttaaagaaac atttaatctc aaatccaaaa
     5941 aggatacaaa gggtatcctg tggtacagtt tggaaactgt taattaattt acggagtata
     6001 gaaatatgtc aaaaaacatt gttgtaccgg aacttggaga atcagtagtc gaagcaaccg
     6061 taattcggtg gataaaaaat gaaggcgagg ctgtttcgat gggtgagaca cttgttgagc
     6121 ttgaaaccga aaaagcaaac ttcgaagtgg ccgcagaggc tgccggagtg ttagagaaga
     6181 ttgttaaaaa cgccgatgag gacgttgtaa taggtgatgt gcttggaaca ataaatgaaa
     6241 acggcacggc ttctgctgaa gtcagcactg aacctgtgca aaccgagacc gctgctgaac
     6301 ctgagcctga gcaggcccag cctaagcctg ctgaacagat tgacgtagat gtaaaggtta
     6361 cccctgttgc aaagaagatt atagagcaaa gcggcttgaa tgcttacgat ataagcggca
     6421 cgggtccaaa cggaaaaata acaaaagaag acgttgaaga tcatataaaa tcagtgagca
     6481 ctgcagcaag cgagaagccc cgtgcaccag agcctgctgc aaagcctgcc ccaagcgccc
     6541 cttctacctc agtggtgatt ccagagccag agtctgacgg aagggaagag cgtgtgagga
     6601 tgtcggcaag aagaagaacc atagcaagaa ggatgctcga ggcccaggca gatgctgcaa
     6661 ttcttactac atttaatgaa gtggacatgg gccccgtgat ggaagtgcgc aagagaagaa
     6721 aggattcatt tgcagaaaaa cacggagtta agcttggctt ttcatcattt tttgtaaaag
     6781 cggtaatagg agcgttaaag gattttcctg ccgtaaatgc agagattcag gacgactata
     6841 tcgtttataa gcactactac gacatcggca tggcaattgg cgcagagggt ggtcttgtgg
     6901 taccggtgct gagggactgt gaccgcatga catttgcgga tatcgaaaga acgatcattg
     6961 agtacgtcgg aaaagcaaac aacaacacgc tttccttgca ggagctaaga ggcggcacat
     7021 ttacaatcac aaacggcgga gtgttcggct ctcttttgag cacgccgatt ctaaacccac
     7081 cacaggttgg aatactcgga cttcataaaa ttgaagagag gccggttgct gtaaacggtg
     7141 aagtggtaat acgccccatg atgtacaccg ccctcagcta cgatcacaga atagtagacg
     7201 gcaaagaggc cgtgcagttt cttgtgaaaa ttaaagagct tattgaagat ccggaagtgc
     7261 tgcttttgga ggggtaattt cttttaagta aattaatgaa ccgactgggt atcttgtact
     7321 gtccctgtta tttcgtattc tccacgacgg gtttttgtga tttttccact aagtgtaaga
     7381 cttgatattg ctcgcttgat tgtagactct gtaaatccct tcagctgaca ttcactttga
     7441 atttcaacag ttgtccactg tggttttctg tccgaaagag atagtatctg ctcctgcacc
     7501 ttaaattttt caaaaccaga cacgctctcg gtgggcggta tatagggctc gaaacggtcg
     7561 tccccgctct ttttaataaa gagagtcata tagtcacccg atatacggtt cttatctacc
     7621 ttgagcttaa gagactgtcc gtcaagtgac tccaggttca ctaccacgtc cacagctcct
     7681 ccgatggcgc ttgctccccg gccgctgtaa acgcccttct tatcggtctt tgagcagtga
     7741 tgtaccagta tcacagccgc ccccgcctcg ctcacaagcc ttcttacaag gtgcatcatc
     7801 ctgtttgcct cggcgttatc gttttcactc tcaagattga atatacggct ctgcgtatca
     7861 aacactatca aatcaaaacc catgtttttg caaaggcaaa gaagctctga gaactgagtg
     7921 tgcatgtcga gagaatcaag aaaataaagc cctgcgggta cttcatctgg agatatgcta
     7981 tttagcttaa cccctctttt ccacccggga gtctcaacgt ctatgtagag caccttgatg
     8041 ctctttgaaa acgggatgtc caaaaacctg cttcctttga ccccgtcaat acacaggtta
     8101 tatacaaacg tggtcttgcc caccccgggc tcagagttta tctgtgtgat gcactgcggg
     8161 taaatcacct caccccagag tgactgaaca gactctgtat taagtgtgat gtcagaagcc
     8221 gccgtgaact taagccctga gcgcatggtg acagatgact ccatctccgc aataagcccc
     8281 catgcctccg gtacactaat tttcccttgt tccaattcct ttgaaataca gctgcacgta
     8341 tcaacaaacg atcgtctctc agatgcgtcc tttaccttct ttacatagta agcgaaattt
     8401 gcagacacgg gcgtggtttc tgtaaggtaa ataagcctct gaggcccgcc aatatttttg
     8461 tctgccttct tttgctttgc atactcaaac accgtcataa ggtctatggg cgcagagttt
     8521 ttgtgcatat cctccagcac ctcaaaaagt tttttgtttg tacttttgta gaagtcatct
     8581 ggcctgagcg ccaccgaaac ctcatccatg atttttggtt cgatcagaat tgaccccagc
     8641 acggcatttt caaactgttc gttagtatct ctaggtatac tcatattttt cctcccttag
     8701 gttttttgtc atatcgggca taattgcaaa aaatgtgtgc taatttgagt cagatttgct
     8761 ttaaaaaggt gtaccatttg aagtaattaa ttctcttgtg tttagccagt cctctatggt
     8821 ttctgtaagc ccatttcact
//
LOCUS       DBNGSV010000213         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_869378, whole genome shotgun sequence.
ACCESSION   DBNGSV010000213 DBNGSV010000000
VERSION     DBNGSV010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_869378"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..281
                     /locus_tag="AC159U_07725"
     CDS             9..281
                     /locus_tag="AC159U_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336985.1"
                     /translation="MNIKIKYDGSYPNLCSGTLIVIIDDNEWLFPSYCLTSGGTVSFT
                     SDWDEVVTYGEWHVSEWPEDFPEELKESVLDVINSEVPQGCCGGCV"
     gene            309..533
                     /locus_tag="AC159U_07730"
     CDS             309..533
                     /locus_tag="AC159U_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336986.1"
                     /translation="MKNIIIILLSVCFLSANINTNVKNDTIKLYQDYIINKHDKKEKR
                     KKTALYIIGTTITASITSFAIYSVKTVPVR"
     gene            537..734
                     /locus_tag="AC159U_07735"
     CDS             537..734
                     /locus_tag="AC159U_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336987.1"
                     /translation="MDKKKYQVQKKYNDELDKIIKKTDNLYRKYAVMMDYTRRSICCD
                     APIYDDTDICSRCKEHTSEIE"
     gene            760..1017
                     /locus_tag="AC159U_07740"
     CDS             760..1017
                     /locus_tag="AC159U_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3336988.1"
                     /translation="MEEYERFYDNLIRESLKEFSESVRINPYRLQLGWQSTYPKYSKM
                     FNAFSDSDKEKIIKLVEEYNNKMPEIKRWRWAIDINRLKGE"
     gene            1035..1631
                     /locus_tag="AC159U_07745"
     CDS             1035..1631
                     /locus_tag="AC159U_07745"
                     /inference="COORDINATES: protein motif:HMM:NF046043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HIR3336989.1"
                     /translation="MPLVAKKPENEGSFELIPAGTHQAVCYGVWDLGHQKTVWNGEEK
                     TQHKVVISWEINKTIQSEGEYKGKRFVISNRYTLSLSEKSNLRKHLESWCGKSLKAYE
                     EKGFDLEQLIGKNCMLSIVHTEKGDKTYANVAAVLGCPDGTQKMTPENGTEPPEWVKK
                     IQSEQVRGEEVVDAALIDNAAFNDADFTPIAEDEIPFG"
BASE COUNT          598 a          267 c          366 g          437 t
ORIGIN      
        1 agtaatacat gaatataaaa ataaaatacg atggctctta tcctaatcta tgtagtggaa
       61 cacttatagt aattatagat gataacgaat ggctttttcc ttcatactgc cttacttctg
      121 gtgggactgt atcatttacc agcgattggg atgaggttgt aacttatggt gaatggcatg
      181 tttctgaatg gccagaagac tttccagaag aattaaaaga atccgtattg gatgttataa
      241 actccgaagt accacaaggc tgctgcggag ggtgtgttta attttacgaa atattaagga
      301 ggactaacat gaaaaacata ataattattt tattgtcggt atgtttctta agtgcaaata
      361 ttaatactaa tgtaaaaaat gatactatta agttatatca ggattacatt attaataagc
      421 acgataagaa agagaaacgg aaaaagacag cactgtatat aatcggtact actataacag
      481 catcgattac aagctttgct atatattccg ttaagactgt accagtgagg taatacatgg
      541 ataagaaaaa atatcaagtc caaaagaaat ataatgatga gcttgacaag attataaaaa
      601 aaacggacaa tctctacaga aaatatgcgg taatgatgga ctacacaaga cgctctattt
      661 gctgtgacgc tcctatctat gacgatacag acatctgttc aagatgtaaa gagcatacca
      721 gtgagataga atgacaaaat acactacaag gggaaagtta tggaagagta tgaaaggttt
      781 tatgataatt taatccggga atcacttaaa gagtttagtg aatctgtacg cataaatccc
      841 taccgtctcc aattgggatg gcaaagcacc tatcctaaat attctaaaat gtttaatgct
      901 ttttcggata gtgacaaaga aaaaataatc aagctcgtag aagaatataa taataaaatg
      961 cccgaaatca agcgttggag atgggcaata gacattaaca ggcttaaagg cgaataacca
     1021 ttaaggaggt aaatatgcca ttagtagcta aaaaaccaga gaatgagggt tcctttgaac
     1081 tcataccggc cgggacacat caggctgtat gttatggagt atgggactta ggacaccaaa
     1141 aaactgtctg gaatggagag gagaaaacac agcataaggt cgtaatatca tgggaaatca
     1201 acaaaaccat tcaatcagaa ggtgagtata agggtaagcg gtttgttatc agtaaccgat
     1261 acaccctctc cctgtctgag aaatccaatc taaggaaaca ccttgagagt tggtgcggta
     1321 agtccctgaa agcctacgag gagaaagggt ttgacctgga acagcttatc gggaaaaact
     1381 gtatgcttag tatagttcat actgagaaag gcgataagac ctatgctaat gtagcagcgg
     1441 ttttaggctg cccggatggt actcaaaaga tgacacctga gaacggtaca gagccaccgg
     1501 aatgggtcaa gaaaatccag tctgaacagg tcaggggaga ggaagtagtt gacgctgctc
     1561 ttattgataa tgcagcgttt aatgatgccg acttcacccc gatagcagag gatgaaattc
     1621 ctttcggtta atggtataat atataatcca atgtgttggg gggtgcaa
//
LOCUS       DBNGSV010000214        16400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1769232, whole genome shotgun sequence.
ACCESSION   DBNGSV010000214 DBNGSV010000000
VERSION     DBNGSV010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1769232"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="nuoH"
                     /locus_tag="AC159U_07750"
     CDS             <1..1071
                     /gene="nuoH"
                     /locus_tag="AC159U_07750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR3336990.1"
                     /translation="DLGFTLLKIGLVLFIVLTLVAYLTLAERKISAFMQDRIGPNRVG
                     PFGLLQPLADGIKFIFKEDVIPDQVNRKFFVVAPAISLVTAIAALAVIPIGKGFYTNF
                     FGVLDEPYFFRFQIADVNVALLYIFAVASLNVYGVVIGGWASNNKYSLLGGLRAAAQM
                     LSYELALGLSVVGVIVLTGSMRLGDIIEAQQNYWFMFPLFIGFIVFIVSSFAETNRAP
                     FDMPEAEPEIVAGYHTEYSSMKFAMFFMAEYTHMIVASCLIVTFFLGGWYPLPFGGWL
                     GIDIDTYWFLPPMVFIGKVLTMLFIFIWVRWSLPRFRYDQVMNLGWKVLLPLSIVNIF
                     ISCGWVYLSERVDLKTLISLNF"
     gene            1575..2012
                     /locus_tag="AC159U_07755"
     CDS             1575..2012
                     /locus_tag="AC159U_07755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3336991.1"
                     /translation="MADTINAILVDEKKISRPGSASIKKASPLRNEQRLYNRIEIKEK
                     IQFGYFKPEYYGISRDITPDGMSIMTDKSLISESNIVVNIYVVDHDKDEDDNINIIEI
                     EGDIIWVKDISDEISHAGVKFKQPNEELIRFYAEKLRDWNNDH"
     gene            2389..2814
                     /locus_tag="AC159U_07760"
     CDS             2389..2814
                     /locus_tag="AC159U_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3336992.1"
                     /translation="MAQTQYAVLNEESPRLVSNARESLLKKEKRLYNRVDINEKVKYG
                     YDKPVHYGISRDITPDGMSIMTDKVLVPRSNIIVNIYIVMKDNNKGEEIKVIEVSGEV
                     IWVKDLSEGLCTAGIKFKQTNEELVRFYAAKLREKVNAL"
     gene            3182..3478
                     /locus_tag="AC159U_07765"
     CDS             3182..3478
                     /locus_tag="AC159U_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR3336993.1"
                     /translation="MSFWVYILRCADNAYYTGHTDNLEKRIGEHQVGEVKGYTSTRLP
                     VALVFSQEFPSREEAKACERQIKGWSQKKKEALIRGDWAEVSRLAKKKHPSTLR"
     gene            complement(3715..6192)
                     /gene="gyrB"
                     /locus_tag="AC159U_07770"
     CDS             complement(3715..6192)
                     /gene="gyrB"
                     /locus_tag="AC159U_07770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3336994.1"
                     /translation="MELEKTLPENQQDSVPENTHGEYTGDQIKVLPGMEAVRKRPDMY
                     IGDTNVRGFHHLVFEVVDNSVDECLAGFADQVTVTIHVDESVTIEDNGRGIPVDIKEE
                     YGKSAAEVVMTTLHAGGKFEGKVYKVSGGLHGVGVTVVNALSEYLVLEIRKEGKVWYQ
                     KYEQGKPLDSLKVSGTTKRNGTKIWFKPDSTIFEINKFSYDILAKRLRELAFLNSGLR
                     VKIVDERTNAEEEFYYEGGIIAFAEELNKNKNTLHPKPIYVTGERDDIIVEVALQYND
                     GYSETVLSYANNINNIEGGTHLTGFRGSLTRTVNKYAEEKGLLKNAKVSLSGDDTREG
                     LTAIISVKLPNPKFEGQTKTKLGNTEVKGIVETLLNEKLAIFFEENPSVARKVIEKTI
                     EAARARDAARKARELTRRKSALESGSLPGKLADCQEKDPGVCELFIVEGESAGGSAKQ
                     ARSRYNQAVLPLKGKILNVEKARFDKMLGNDEIRTLITVLGTGIGADDFDIAKLRYHK
                     IILMTDADVDGSHIRTLILTFFYRHLPEIIERGYLYVAQPPLYRIKKGKDERYLKDDP
                     EFEDYILNIGNESVVMNVKSNGESKEIKGARLLEIIKKSTNYENILNRLSKLGRDSRI
                     LDAVASANGFRKTYLKVGNESDLDTNLKHIKEWINRIYPEVTDLDWTLEEDSEHNASK
                     IIFTFRDTGRTIQTTIDFDFLNSPDFAEIKNLRESIKAVSEPPYLLTEDGEETSLGSL
                     NEVSDYILNKGKKGMNIQRYKGLGEMNPDQLWETTMDPEKRILKQVHIEDAIKTDEIF
                     TILMGDQVEPRKHFIETNALNVTNLDI"
     gene            complement(6310..6870)
                     /gene="dcd"
                     /locus_tag="AC159U_07775"
     CDS             complement(6310..6870)
                     /gene="dcd"
                     /locus_tag="AC159U_07775"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3336995.1"
                     /translation="MSVKPDHWIRKMSLEHKMIDPFVEGQVSKGVISYGLSAYGYDVR
                     IGDEFKVFTNVHNTVVDPKDFNDKSFVTIKADSCIIPPNSFALAQTIEYFRIPRSVIT
                     VCVGKSTYARCGIIVNVTPFEPEWEGFVTLEVSNTTPLPAKIYANEGIAQVLFFESDE
                     VCETSYADKKGKYQKQVGITPPKVIK"
     gene            complement(6901..7917)
                     /gene="pheS"
                     /locus_tag="AC159U_07780"
     CDS             complement(6901..7917)
                     /gene="pheS"
                     /locus_tag="AC159U_07780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3336996.1"
                     /translation="MEEKLEELKNQFTEELKDISDITSLSVLKAKYLGKKGAVTDILK
                     GMKDLTAEQRPLMGKLVNEIKNVLESGLDAKEKEITEAQKKKILQEEKIDVSLPPRGM
                     PLGTLHPITQVMNEIVDIFEHMGFEVAEGPEIETDFYNFEALNIPKNHPARDMQDTFY
                     ISDDVVLRTHTSPVQIRVMEKRQPPIQIIAPGKVYRCDSDVSHTPMFHQVEGLLVNET
                     VTFADLKGVLSEFCKLIFGGDTKVRFRPSFFPFTEPSAEVDISCVICGAKGCRVCKNT
                     GWLEILGCGMVDPEVFRSVNYDSTKYRGFAFGMGVERITMLKFGINDIRLFFENDLRF
                     LKQF"
     gene            complement(7922..8422)
                     /locus_tag="AC159U_07785"
     CDS             complement(7922..8422)
                     /locus_tag="AC159U_07785"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HIR3336997.1"
                     /translation="MDILAVTLFIAFALAGIFCTFIGLPGNFIILISSGLLAWYFGFE
                     VIKINIIILLVSLAVLGEVLEFVIGIIGSKSKKASNKAVVGSIIFSIIGAIMFAPVMF
                     GLGAILGAFLGAFFGAFIVEFIRFKDAKKAVESGKGAFLGKLGGVLVKTVIGFIMITT
                     TLLQVF"
     gene            complement(8439..9353)
                     /gene="secF"
                     /locus_tag="AC159U_07790"
     CDS             complement(8439..9353)
                     /gene="secF"
                     /locus_tag="AC159U_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3336998.1"
                     /translation="MDIIKPGTIVDFISKMRTAFIISAVLIAISVISLFVHKGLNWGV
                     EFIGGTEVHMKFQQDINSLEIKERLAGINYDDVSVQSLGLEGDNEYIIRLSSDVVKHN
                     QIKDFQKQIEGLVVGEKAGKFKDADILRIDFIGPKVGSELIKKAVGAIIFGWLGILIY
                     VMLRFEIGFALGAVIAIIHDVFITLGAISIMDKEFNLAIVAAFLTVIGYSVNDTIVIF
                     DRIRENMSRGYSSFSTLINESVSQTLSRTILTTLTALIVLACLFVFGGSVIHDFAYTL
                     MVGVIAGTYSSIFVATAFVVYWNKNREL"
     gene            complement(9379..10977)
                     /gene="secD"
                     /locus_tag="AC159U_07795"
     CDS             complement(9379..10977)
                     /gene="secD"
                     /locus_tag="AC159U_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3336999.1"
                     /translation="MRRNTKSWLIIISFISVISLILLAPTFVGDSLPQWWSKVFPERG
                     IRLGLDLRGGIFLMLDVQKEEAIGHELSSIKNLVTDDLLKDKVLIKDTRVDEKSVSFD
                     FFTKNDLAKAKKVVDTYSETADIKENNLTLTVVLKNSTIENLQKDAIKQVMSVIVNRI
                     SEFGLIEPTIQQAGEDRILIQVPQASDKDRERIISIIRKAAVLEFKIVEDVSAVQEPL
                     LSKYEVTTPEELIEKGYMLHPGETGTENEKFFITEAHARVSGKYLSDARLIFDQFGRA
                     AVGFSFRGEGADAFYETTKSNINRRLAIVLDGRIKSAPNIEGAIRSSGQITGNFTPEE
                     ARDLALVLRSGSLPVSVNIEQERTVGPSLGKDSIEKGKFSMLLGGLLVFIFMVIAYKK
                     QGLIADIALGLNVLFIMGALSAFGVTLTLPGIAGLVLTLGMAVDGNIIIFERIKEELR
                     GGKVPLNAIDSGYGRSLWTILDANITTLITALILFWFGTGPIKGFAATLSIGIVATVF
                     SNVIVARVFTNLIHSDKKMKTLSI"
     gene            complement(11000..11368)
                     /gene="yajC"
                     /locus_tag="AC159U_07800"
     CDS             complement(11000..11368)
                     /gene="yajC"
                     /locus_tag="AC159U_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3337000.1"
                     /translation="MKLRNIIFTLPFLFLIGCIPQGGGAGGGSSSMIGGIIPFVLIFV
                     LFYFLILRPQQKQTRERDNMIKNLKRGDNVLTNGGIYGKITNINEDILSVEIAKGLNI
                     KMTRNGIASLANESQEEKKS"
     gene            complement(11370..12476)
                     /gene="tgt"
                     /locus_tag="AC159U_07805"
     CDS             complement(11370..12476)
                     /gene="tgt"
                     /locus_tag="AC159U_07805"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3337001.1"
                     /translation="MYNFEITKKDSSTSARTAVISTAHGKVQTPAFMPVATLGTVKSI
                     TPEELEELGFSMIISNAYHLYLRPGHKEIKDLGGLHRFMNWNNSIATDSGGYQVLSLS
                     KKRKIKDDGIVFQSHLDGSEHFLTPKLSIEIQEHVGSDIMMCLDECPPYSDDYKSIDK
                     SLELTTRWAKLCKESRTRDDNALFGIVQGGVFTDLRKKSAEQLIEIDFDGYSIGGLGI
                     GEDPLKTHDLIAHTASLLPEDKTRYAMGIGNPVDIVKAAAGGVDLFDCVIPTRNARNG
                     TLFTNTGKLVIKNAQFTNDSDPIDQECSCYTCKNYSRAYIRHLYICKEILAIRLLTMH
                     NLSFFGNLMKSIRSSLKDGNFSELIKKYESTEVN"
     gene            complement(12515..13321)
                     /gene="dapB"
                     /locus_tag="AC159U_07810"
     CDS             complement(12515..13321)
                     /gene="dapB"
                     /locus_tag="AC159U_07810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3337002.1"
                     /translation="MAVIRAGVVGVGGKMGERILSLLLENDDFEIAGATEVARSRLIG
                     KDVESLSAQKKTSVTVSSTLDEAFSSADGIIDFTSPESTLKTAEYAAQNSKALVVGTT
                     GFTEEQKLQLTKLAYNFPCVFSPNMSVGVNVMFEMSKKLTELLGTDFDIEILEAHHRN
                     KKDSPSGTALRIAEVVSETLDKKLNDIAVYSRHGNIGARKKGELGIQTLRGGDVVGEH
                     SLIFYGDGERIEFTHKASSRDTFANGAIRALRWVHDKPAGIYTMKDVLGF"
     gene            complement(13325..13744)
                     /locus_tag="AC159U_07815"
     CDS             complement(13325..13744)
                     /locus_tag="AC159U_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3337003.1"
                     /translation="MTACAGTSPQSTTSTQSSSGKNSPSYVVTKFLDAFVKNDFGSAF
                     KYIHSNATDRQGYESRMKDLVETSNNNITNYNLIGTRIIGEIAYTVVELEIEEKSGGG
                     NTKIRYTKNQYELSFIDGKWKIVKDHGCVENCTDKKG"
     gene            14003..15571
                     /locus_tag="AC159U_07820"
     CDS             14003..15571
                     /locus_tag="AC159U_07820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR3337004.1"
                     /translation="MDSVLAGNLSLVIFFPLIGTLFLVLLSLVKKDADEELKIGALVI
                     SLIEFLISLPLFCNFQKGFAGLQFETNIPWLAEFGINYHIGIDGISVFLVLLTTFIMP
                     ITILSAWNAIHKGLREFLIFMLVLETAMIGTFSSLDMVLFFVFWEAVLIPMYFLIGIW
                     GTERRIYAAIKFFIYTAFGSALMFIAILYLHKLHMDQFGIATMNIFDFYNLSIPYAGI
                     LSMQGLLFLAFFLAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAAVLLKMGTYGFLRF
                     LMPLFPEATIDLLPILCSIAVIGVIYGALVAFAQKDLKKLVAYSSVSHLGLVMLGVLV
                     LNIQGVQGGIYQMLNHGLSTGALFLIVGMVYERRHTKKIAEFGGIAKVMPILALFFML
                     ATLSSIGLPLLNGFVGEFLILLGAFKWDGRFSVLGTTGIVLGAIYMLWAYQRVMFGPL
                     NKPENKALRDLSVREIFVLTPIAIMFFVMGIYPKPFLERIEPSVNSLIHTKFAETIKQ
                     DDSENVLSQYFRGR"
     gene            15906..>16400
                     /locus_tag="AC159U_07825"
     CDS             15906..>16400
                     /locus_tag="AC159U_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR3337005.1"
                     /translation="MSYSVEGQLLEVCICNVICPCWVGGDPDEGYCDGMIGWHIDQGE
                     IEGVNVSGCNFAMLLHSPGNVLDGNWKVRLYVDEELSDEQQEGILNLWGGKLGGAITD
                     LASLVGEVASVEKTHIRFDVEGVNGTIAIGDVVSASLEAFKGATGAETTLHNSVFTSI
                     PGKPA"
BASE COUNT         4367 a         3509 c         3291 g         5233 t
ORIGIN      
        1 gatcttggat ttacgcttct taaaattgga cttgtactat ttatagtgct cactctggtt
       61 gcgtacttaa cacttgcaga gagaaaaata agcgcattca tgcaggacag aatcgggcca
      121 aacagggtag ggccgtttgg gttattacag ccccttgcag acggcataaa gtttatattc
      181 aaagaagacg tcatacccga tcaggtaaac aggaagttct ttgttgtagc tcctgcaatc
      241 tctcttgtta cagcaatagc cgcgcttgca gtaattccca taggtaaggg attttatacc
      301 aattttttcg gtgtgctgga cgagccgtac tttttccgct tccagattgc tgatgtaaac
      361 gtggctctgc tctatatatt tgcagtagca tcgctcaacg tctatggagt agtaataggc
      421 ggatgggcgt ctaataacaa gtattcgctt ctcggcggcc tgcgtgcagc ggcacagatg
      481 cttagttatg agcttgctct tgggctatct gttgtgggcg taatagtgct tacggggtcg
      541 atgaggcttg gcgacattat agaggcacag cagaactact ggtttatgtt tccgcttttt
      601 ataggattta tagtgtttat agtctcgtca tttgcagaga caaacagggc gccttttgat
      661 atgcccgagg cagagcctga gatcgttgcc ggctatcata cagagtacag cagtatgaag
      721 tttgctatgt tcttcatggc tgaatacacg catatgattg tggcttcttg tttaatcgtg
      781 accttctttt taggcggctg gtatccgctt cctttcggag gctggcttgg gattgatata
      841 gacacatact ggtttttacc gcctatggtc tttattggta aagtactgac catgctattt
      901 atattcatct gggtgcgctg gtcacttccg cgctttcgtt acgatcaggt gatgaacctg
      961 ggctggaagg tgcttctgcc tctttccatt gtaaacatat ttatttcctg cgggtgggtt
     1021 tatctctccg agagggttga tctcaaaact ctcatttccc ttaatttcta gttttttccc
     1081 acaattgtca tgcttgagcg taagcgaagc atctcgattt tagattcttc ggctccatct
     1141 cagtatgaca catccactta ataatacacc gagcatgtcc tgcggatcag atacaacaga
     1201 taataaaaga aactaaacaa tattatccaa aaattgccat tatcttccaa aaattgtcag
     1261 ttatttccca aatttgtgta aattattttt ctaacttccg ttcaaccctt atttagaaga
     1321 acatatactc atacatatac ctgtttgggc agatactttc ctaaacaact tcaaataaat
     1381 gactatttaa tataatgctg tactgcatta cctgaaatga tgatcatgtt ggtaaaaatt
     1441 ggcttaaaat ctgcctgttt agtatcaaat atcgatatat gcgacactgc ttctctcata
     1501 tttttgtatg gcacaaagat tgctctttat gtaagtaaag aacgctaaat atccaaaatt
     1561 gcgaggtaat aataatggca gatactataa atgctattct agtagacgaa aaaaagatat
     1621 ccagaccggg ttcggcatca atcaaaaagg caagcccgct aagaaatgag cagcgtttgt
     1681 acaacaggat tgagattaaa gaaaaaattc aatttggata ttttaaacca gaatattatg
     1741 gtatttcgcg tgatattact cccgacggga tgagcattat gactgataag tcgctaatat
     1801 cagagtcaaa tatagtggta aatatatatg ttgtggatca tgataaggac gaagatgata
     1861 acattaatat cattgagata gaaggtgata ttatttgggt aaaagacata tcagacgaga
     1921 tttctcatgc aggggttaaa ttcaagcagc caaatgaaga attgatcaga ttttatgcgg
     1981 aaaaattaag ggactggaat aatgatcatt aaataataaa tcggattccc cctaagttcg
     2041 atttataagg cagtcacggt tagccctgac tgcctttttt ttgtgccgtt ttcataagtc
     2101 attctgagta cttcggtatg ctcagcacag actcggggaa gaacttcatg atccatacta
     2161 agggaaatgt ttccctaaat gagttcaatt acatggctat ttaataaaat actgcacagt
     2221 atttcctgga ttgaaggtga aagtggtaaa aattagctta aaacctgcct gaatagctac
     2281 aattataggc atatacgacc ccgattcttt tatattttca tatggcacga agattgctct
     2341 atatgtaagt aaagagcact aaatatcaaa aatagtgagg aaagtataat ggcacagact
     2401 caatatgcag tacttaatga agagagccca aggcttgtat caaatgcaag ggaatcactg
     2461 ctaaagaagg aaaaacggtt atataacaga gtcgatatca atgaaaaagt taaatatggg
     2521 tatgataaac ccgtgcatta tggcatatcg cgcgatatta cccctgacgg gatgagcatt
     2581 atgacagata aagtgcttgt tccaagatcg aatataatag tcaatattta tattgtaatg
     2641 aaggataata acaaaggtga agaaattaag gttattgagg tttctggaga ggtcatttgg
     2701 gtaaaagatc tttcggaagg cctttgtaca gccggtatta aatttaagca gacaaatgaa
     2761 gagcttgtaa ggttctatgc agccaagctg agagaaaaag taaatgcgct ataaattgta
     2821 aaaaaagccc tactcttgat tcataaggta gtcacggttt gctccctgac tgcctttttt
     2881 tgtgccgttt tatctggttc ttatccaaac acacttgccc tgaccccaat tgtggttgag
     2941 atcagttact ataactgtgt atactagtta tgaaataaat ggggatttta ccctttaaaa
     3001 aatcatcctt cgacaagctc aggatgaacg gattgatata agatcatgat atttgaatag
     3061 gcatctccga tgaccctgag tcctgagttt atcgaagggt tggggtgtga ggatgaagga
     3121 tctaatattt tgggttgtaa gaggatatga attcacaaaa attaggaaat accaatagaa
     3181 tatgagtttt tgggtttaca ttttacgctg tgcggataac gcatactaca cggggcacac
     3241 ggataacctt gagaagcgaa taggtgagca tcaggtaggt gaagtcaaag gttacacatc
     3301 cacaagattg cctgtggcgc tagtattttc acaggaattt ccttcccgtg aggaagccaa
     3361 agcctgtgaa aggcagatca aaggatggag tcagaagaaa aaagaggcat tgatacgtgg
     3421 agattgggct gaggtgtctc gcttagcaaa aaaaaagcat ccttcgaccc ttcgataaac
     3481 tcaggactca ggactatttg tggggtgtga gccgttttca aagctcaaaa ctcaggatgg
     3541 agaagtgttg aaaaaaagcc gttcacactg agcatgtcga agtgtgtatc tacgctgaaa
     3601 catgtttaac caatacatac ttcatgttta accaacacat acttagaggc ctaaatgcat
     3661 ttggataggg aattaaataa taaaagaaaa agatgtaact aaatcaggtt tggactaaat
     3721 atccagatta gtaacgttaa gggcgtttgt ttctatgaag tgttttctgg gctctacctg
     3781 atcacccatt agtattgtga agatttcgtc tgtcttgatt gcgtcttcga tatgtacctg
     3841 ttttaaaatc ctcttttcag gatccatggt tgtttcccag agctgatcgg ggttcatctc
     3901 accaagccct ttatagcgct ggatgttcat gcccttctta cctttgttaa gaatataatc
     3961 cgaaacttcg ttaaggctgc cgaggcttgt ttcctcgcca tcttctgtga gcaggtaagg
     4021 cggctcggat acggccttta ttgattctct taagtttttg atctctgcaa aatcaggaga
     4081 gtttagaaag tcaaagtcta ttgttgtttg tattgtcctt ccggtgtctc taaatgtgaa
     4141 tatgatcttg gatgcgttgt gttcggagtc ttcttccagg gtccagtcaa ggtctgtgac
     4201 ctcggggtat atcctgttta tccactcttt tatatgtttt aaattagtat cgaggtcaga
     4261 ttcatttcca accttaagat aggtctttct aaaaccgttt gcgcttgcca cggcgtctag
     4321 tattctggaa tctcttccaa gcttgctgag cctgtttagt atattttcat aatttgtaga
     4381 tttttttatt atttctaaaa gccttgctcc cttgatttcc tttgactcac cgtttgattt
     4441 tacattcatt acaacagact catttccaat gttaagtata tagtcttcaa actctggatc
     4501 gtccttaagg tatctctcat ccttgccttt tttaattctg taaagcggcg gctgagccac
     4561 atacaggtag cccctttcaa taatctccgg caggtgacga tagaaaaagg ttaaaataag
     4621 cgttctaatg tgagagccgt ctacatcggc atcggtcatt aatattattt tatgatacct
     4681 aagctttgca atatcaaagt catcggcgcc aatgccagtt ccaagaactg ttatgagtgt
     4741 ccttatttca tcgtttccaa gcattttatc aaaccgggcc ttctctacat ttagtatctt
     4801 accctttagc ggaagcacgg cctggttgta tctggatcgg gcctgttttg cagagccccc
     4861 ggctgattca ccctctacta taaatagctc gcatacaccg gggtcttttt cctgacagtc
     4921 ggcaagtttt cccggaagcg agcctgactc taaagcgctc tttcttcttg taagctccct
     4981 ggcttttctg gctgcgtctc ttgcccttgc tgcctcaatt gtcttttcga ttacttttct
     5041 tgctactgaa gggttttctt caaagaatat ggctagtttt tcgtttaata gagtttccac
     5101 tattccctta acttcggtgt tcccaagctt ggtctttgtc tgtccttcaa acttgggatt
     5161 tggaagcttt acgcttatta tggctgtaag cccctcacgt gtgtcgtctc cgctaaggga
     5221 tacctttgcg tttttaagaa gccccttttc ttcggcatat ttgtttacag tccttgtaag
     5281 cgagccccta aaacccgtta agtgagtacc gccctcaatg ttattaatgt tgtttgcata
     5341 agaaagtaca gtttcagaat aaccatcgtt gtactgcagg gctacttcta caataatgtc
     5401 atccctttcg cctgtaacgt atatgggttt tggatgaagc gtgtttttat tcttgtttag
     5461 ttcctctgca aaagcaatga taccgccttc atagtaaaat tcttcttcgg cattagtgcg
     5521 ctcatctacg attttcactc ttagtccgct gtttagaaat gcaagctctc ttagcctctt
     5581 tgcaagaata tcgtagctga atttgtttat ttcaaagata gtgctgtcgg gtttaaacca
     5641 tatttttgtg ccgtttcttt ttgtggttcc agaaaccttt agagaatcga gcggtttgcc
     5701 ctgttcgtac ttttgatacc agacttttcc ctcttttcta atctcaagca ccaaatactc
     5761 tgaaagcgca tttactaccg ttacacccac tccgtgcaga ccgcctgata ctttgtatac
     5821 ctttccttca aactttcctc ccgcatgaag cgtggtcatt acgacctctg ctgcggactt
     5881 gccgtactct tcctttatgt ctacgggaat gcctcttccg ttatcttcga tggttacgct
     5941 ttcgtccaca tgaatggtta ctgtgacctg atctgcaaac cccgcaaggc actcatctac
     6001 actgttgtct accacttcaa aaaccaggtg gtgaaaccct cgtacgttgg tatccccaat
     6061 gtacatatcg ggtctcttgc gtacagcttc cattcccggg agtaccttaa tctggtctcc
     6121 ggtatattcg ccgtgagtat tttcaggcac gctgtcctgc tgattttctg gaagggtttt
     6181 ttctagttcc acctaaaaag tttccatgta atacaaagta caaatatgat acccgcaatt
     6241 aatagaatgt aaacaaagca atagcagttt tttaatattg atactggcaa aaaaagctaa
     6301 tgatttaggc tatttaataa cctttggcgg ggtgatgccg acctgcttct gatactttcc
     6361 ttttttatcg gcataagatg tctcacaaac ctcgtctgat tcgaaaaaca gcacctgcgc
     6421 aataccctca tttgcataga tttttgccgg aagcggagtg gtgtttgaga cctcaagagt
     6481 tacaaatcct tcccactcag gctcaaacgg ggttacattt acaatgatac cgcacctggc
     6541 ataagtggac ttaccaacgc acactgttat aacgcttctg ggtatcctga aatactcaat
     6601 agtctgtgcc agagcaaacg agtttggtgg gataatacac gaatctgcct taatagtcac
     6661 aaaggattta tcgttgaagt ccttcgggtc tacaaccgtg ttgtgaacat tagtaaacac
     6721 tttaaactca tcacctatcc ttacgtcata cccataggct gatagtccgt atgaaatgac
     6781 tccctttgat acctgtccct caacaaacgg gtcaatcatc ttgtgctcaa gcgacatctt
     6841 tctaatccag tgatccggtt ttactgacat ttgctgctcc ttatattttc ttttttagtt
     6901 ttaaaactgc tttaaaaacc ttaggtcatt ttcaaaaaac agtcttatat cgtttattcc
     6961 aaatttaagc atcgtaattc tttccacacc catgccaaag gcaaaacctc tgtatttggt
     7021 gctgtcgtaa tttacgctcc taaacacctc gggatcaacc atcccgcagc caagaatctc
     7081 aagccatccg gtatttttgc acaccctgca ccctttggct ccgcagatta cacagcttat
     7141 gtccacctcg gcgcttggct cagtgaaggg aaagaagctt ggcctgaagc gaacttttgt
     7201 atccccgccg aagatgagct tgcaaaattc ggaaagtaca cctttcaggt cggcaaaagt
     7261 tacggtttcg ttcacaagca ggccctcaac ctgatggaac atcggcgtgt gtgacacgtc
     7321 actgtcgcat ctgtacacct taccaggcgc aatgatctga atcggaggct gccgcttttc
     7381 cataaccctg atctgcacgg gagaagtatg tgttcttagc accacgtcat cacttatata
     7441 aaaagtgtcc tgcatgtctc tagcagggtg gttctttggg atattaaggg cctcgaagtt
     7501 ataaaagtcc gtttctatct caggcccttc ggcaacctca aagcccatat gctcgaatat
     7561 atccacaatc tcgttcatga cctgagtaat ggggtgaaga gtgccaagcg gcattcctct
     7621 gggaggaagg cttacatcga ttttttcttc ctgcagtatc tttttctttt gagcctcggt
     7681 gatctccttt tcctttgcat cgaggccgct ctcgagtacg ttctttattt catttaccag
     7741 ttttcccata aggggacgct gctctgctgt aagatctttc atgcctttta agatgtctgt
     7801 tacagcacct tttttaccaa gatatttagc ttttagcacc gaaaggctgg ttatatcgga
     7861 aatatcctta agctcttcgg tgaactggtt tttaagctct tctaattttt cttccatatt
     7921 tctaaaatac ctgaagtaga gttgttgtaa tcatgataaa tcctattact gtttttacga
     7981 gtactccgcc cagttttccc aaaaacgctc ccttgccaga ttctactgcc ttttttgcgt
     8041 ccttaaatct tataaattca actataaagg ctccaaaaaa agcgcccagg aaagctccta
     8101 aaattgcccc caggccaaac attacgggag caaacattat agcccctata atagaaaaaa
     8161 tgatagagcc tactacagcc ttattgctcg cttttttgct cttagatccg attatgccaa
     8221 tcacaaattc gagcacctca ccgagtaccg caagtgagac gagtaaaata ataatattta
     8281 tttttataac ttcaaatcca aaataccagg ctagcagtcc tgatgaaatt aaaattataa
     8341 agtttccggg aagtccaatg aatgtgcaaa aaattcctgc aagggcaaac gctatgaata
     8401 atgttacagc taatatatcc acatcaattt atcaaccatt aaagctccct gttcttgttc
     8461 cagtacacta caaacgcagt ggccacaaaa atagaggaat atgtacctgc gattacaccc
     8521 accattagcg tgtatgcaaa atcatgtata accgatccgc cgaacacgaa caggcatgca
     8581 agcactataa gagcagtaag agttgtgagt atggttcttg aaagagtctg tgagacgctc
     8641 tcattaataa gcgttgaaaa ggacgaatag cccctgctca tgttttccct tatcctgtcg
     8701 aatataacaa tcgtatcgtt tacggagtat ccgatcactg ttaaaaaagc cgctactatt
     8761 gcaaggttaa attccttgtc cataatcgat attgcgccca gggttatgaa gacatcatga
     8821 ataatcgcaa tcacggcacc tagcgcaaag cctatctcaa agcgcagcat tacataaatt
     8881 agtattccaa gccatccgaa tattattgcg cccacagctt ttttgataag ctcacttccc
     8941 acttttggtc ctatgaaatc tattctgagt atgtctgcgt ctttaaactt tccggctttt
     9001 tcaccaacga caagcccttc tatctgtttt tgaaaatctt ttatctggtt gtgttttact
     9061 acatccgaag agagccttat gatgtattcg ttgtccccct caagaccaag cgactgtacg
     9121 cttacatcat cgtaattaat ccccgcaagt ctttccttta tttctaaaga gtttatatcc
     9181 tgttggaatt tcatgtgtac ttctgtgccg cctataaact ctacgcccca gttgagtccc
     9241 ttgtgtacga aaagtgaaat aacagagata gctataagaa ctgccgagat aataaacgca
     9301 gtcctcattt tgcttataaa atctactata gtgcctggct taattatatc cacgttaaag
     9361 aatacctcct gagggtaact atatgctaag tgttttcatt tttttgtctg agtgtataag
     9421 gttagtaaat acccttgcca cgattacgtt gctaaacacg gttgctacga ttcctattga
     9481 aagagtggca gcaaaacctt ttatgggtcc tgtaccaaac caaaacagta tgagcgctgt
     9541 tataagagta gtaatgtttg catccaatat tgtccaaagc gacctgccgt aaccagagtc
     9601 aatcgcattt agggggacct tgccgcccct tagctcctct tttattcgct caaatattat
     9661 tatgtttcca tccacagcca taccaagagt aagtacaaga cctgcaatac caggaagcgt
     9721 gagtgtaaca ccgaaagccg aaagcgctcc cattataaat agtacgttta gtccaagagc
     9781 aatatcagcg ataagtccct gttttttata ggcgattacc ataaaaataa atactaacag
     9841 tccgccaaga agcatagaaa acttaccttt ttcaatggag tccttaccaa gcgacggccc
     9901 cactgttctt tcctgctcaa tattcacgct tacaggaagc gagccagacc ttagaacaag
     9961 tgctaagtcc cttgcttctt cgggggtaaa attgccggtg atctgaccac ttgatcttat
    10021 agctccttca atattgggcg ccgatttgat ccttccgtca agcacgatgg caagccttct
    10081 gtttatgttg gattttgtgg tttcataaaa tgcatcggct ccttctcccc tgaatgaaaa
    10141 acctacggct gctcttccaa actgatcgaa tatgagtctt gcatctgaga ggtatttacc
    10201 cgataccctc gcatgtgcct ctgttataaa aaacttttca ttctccgtac cggtttctcc
    10261 ggggtggagc atgtagcctt tttctattaa ttcttctggt gttgttactt catacttgct
    10321 gagcaagggc tcctgcacag cggatacgtc ctctacaatc ttaaactcga gcaccgctgc
    10381 ctttcttata attgaaataa ttctttccct gtccttgtct gatgcctggg ggacctggat
    10441 taaaatcctg tcttctcctg cctgctgaat tgtgggttca atgagcccaa actcgcttat
    10501 acgatttaca ataacgctca ttacctgctt aattgcatcc ttttgcaggt tttcgattgt
    10561 agaatttttt agaactaccg tgagtgtgag attgttttcc ttaatatccg ctgtttcgct
    10621 gtaagtatct actacctttt ttgcctttgc gaggtcgttt ttggtaaaga agtcaaagct
    10681 tacggatttt tcatcgactc ttgtatcctt aataagaacc ttgtctttaa gaaggtcgtc
    10741 tgtaacaagg tttttaatgc tggatagctc atggcctatg gcttcttcct tctgtacgtc
    10801 tagcataaga aatatcccgc ccctaaggtc aagcccaagc cttatgcctc tttcaggaaa
    10861 taccttagac caccactggg gaagtgaatc acctacaaaa gtgggtgcaa gcaggatcag
    10921 tgaaataaca gatatgaatg atattattat aagccaggat ttagtatttc ttctcatcta
    10981 tcggattttc tccctcactt tatgattttt tctcttcttg cgattcattt gcaaggcttg
    11041 caatgccatt tctggtcatc ttgatgttca gtccctttgc aatctctacg cttaagatat
    11101 cttcatttat atttgtaatc tttccgtata tcccgccgtt agtaagaacg ttgtccccgc
    11161 gctttaggtt tttaatcatg ttgtcacgct cgcgtgtctg tttttgctga ggtcttagaa
    11221 ttagaaaata aaacagcacg aatatcagta cgaatgggat tatgccgccg atcattgagc
    11281 tgctgccccc tcccgctccg cctccctgcg gaatacatcc aataaggaat aaaaatggta
    11341 gggtaaaaat aatatttctt aatttcaatt taattaacct ccgtagattc atacttcttt
    11401 ataagctctg agaaatttcc atccttcaag ctgcttctta tacttttcat caggtttcca
    11461 aaaaaactaa gattatgcat ggtaagtagc cttatggcca gaatttcttt gcaaatatat
    11521 agatgtctta tataagccct tgaataattt ttgcatgtat aacagctaca ttcctgatca
    11581 atcggatccg agtcgtttgt aaattgagca ttttttataa ccagttttcc tgtgtttgta
    11641 aagagggtgc cgtttcttgc gtttcttgtg ggaattacac agtcaaaaag atcgactccg
    11701 cctgctgctg ccttgactat gtctacgggg tttccaatgc ccatggcata ccttgtttta
    11761 tcttccggaa gaagggatgc tgtgtgggca ataaggtcat gagtcttaag aggatcttca
    11821 ccaattccaa gcccgcctat ggagtagccg tcaaaatcta tttcaattaa ctgctctgcc
    11881 gatttttttc ttaagtcagt aaatactccc ccctgcacga ttccaaaaag cgcattatcg
    11941 tctcttgttc gagactcctt gcagagtttt gcccaccggg tggtaagctc aagcgattta
    12001 tcaatgctct tgtaatcatc agaataggga gggcattcat caaggcacat cattatatca
    12061 gagcctacat gctcctgaat ttctatgctt agctttgggg taagaaagtg ctccgagccg
    12121 tcgaggtgag actgaaacac aatgccgtca tcttttatct ttcttttttt tgagaggcta
    12181 agcacctgat acccgccgct gtctgttgca atggaattgt tccagttcat aaacctatgg
    12241 agcccgccaa ggtcttttat ctctttgtga ccgggtctca ggtagagatg ataggcgttt
    12301 gatattatca tgctaaaccc cagttcttcc agttcttctg gggtaattga tttcacagtt
    12361 ccaagagtgg caacaggcat aaaagccggg gtttggactt taccgtgggc cgtggatatt
    12421 actgctgttc ttgcacttgt agaactgtcc ttttttgtga tctcgaaatt gtacatatgg
    12481 tattggggtt taattataac aatagaggga aagtttaaaa acctagtacg tctttcatgg
    12541 tgtatatccc tgccggttta tcatgcaccc accgaagcgc ccttatggct ccgtttgcaa
    12601 atgtatccct gctcgatgcc ttgtgagtaa attctatcct ctctccgtct ccataaaata
    12661 tgaggctgtg ctcgccaacc acatcgcctc cgcgaagggt ctgaattcca agttctcctt
    12721 tttttctcgc acctatgttt ccatgccttg agtatactgc aatgtcgttt agtttcttat
    12781 caagagtctc cgataccacc tcggcaatcc tgagcgccgt gccgcttggg gagtcttttt
    12841 tgtttctatg atgggcctct aaaatttcaa tatcaaaatc tgtgcctaaa agttcagtta
    12901 gttttttgga catctcaaac atcacattta ctcccacgct catgtttgga gaaaaaacac
    12961 atgggaaatt atatgcaagt ttggtaagct gtaatttttg ctcttcagta aaacccgtgg
    13021 tacctactac aagagccttt gagttttgtg ccgcatattc tgcagtctta agtgtggact
    13081 cgggtgatgt aaaatctatt attccgtcgg cactgctaaa cgcctcatcc aaagtgctgc
    13141 ttaccgtaac gctggttttc ttttgggcgc taaggctttc tacatccttt ccaatgagcc
    13201 tgctgcgggc aacttcggta gcccctgcga tctcaaaatc atcattttcc agtagcaacg
    13261 agagtatcct ctcacccatc tttccaccga ctcccactac gcctgctctt atcactgcca
    13321 ttttctaacc ctttttatcc gtacagtttt ccacgcatcc gtggtctttt actatcttcc
    13381 atttcccatc tatgaagcta agctcatact gattctttgt ataccttatc ttggtgtttc
    13441 cgccgcctga tttttcttct atctcaagct ctactaccgt ataagctatc tcaccaataa
    13501 tacgcgtgcc gatcagattg tagtttgtga tattgttgtt tgatgtttct acaaggtctt
    13561 tcatcctcga ttcataaccc tgcctgtctg tagcatttga atgaatgtat ttaaatgcag
    13621 agccgaaatc attttttaca aaggcatcca gaaatttggt taccacgtag cttgggctgt
    13681 ttttgccact tgaactctgg gtgctggttg tactttgcgg gcttgtgcca gcgcatgcag
    13741 tcatcaaaat tatgaatact aaaggaatga gtctttgcat agttatagtt tgtttagtaa
    13801 tgataataat attctctccg tttataatat tcaacacaac agcttacaga aggtttcagc
    13861 tactatttac agtttgttaa aacattaatg gactaatata ttacactata ttttgtagtt
    13921 ctaaattaat tatttacata tttactattt tgtatattct actgccagaa tttgataaaa
    13981 aattggaggc tttgtaatag ttttggactc tgttcttgct ggaaatcttt cccttgtaat
    14041 attctttccg cttatcggta cgctgttttt agtacttctt tctcttgtta aaaaagacgc
    14101 ggatgaggag cttaaaatag gagctctcgt aataagcctc atagaattct taatttcact
    14161 gccgcttttt tgcaattttc aaaagggctt tgcaggcttg cagtttgaaa ccaatatccc
    14221 gtggctcgca gagtttggta tcaactatca tattggcata gacggcataa gcgtctttct
    14281 tgttcttctc actaccttta ttatgcctat taccatcctc tcggcctgga acgctataca
    14341 caaagggctt agagaatttt tgatattcat gcttgtgctc gagactgcca tgataggcac
    14401 attcagctct ttagatatgg tgctgttctt cgtgttctgg gaagcagttc ttatcccgat
    14461 gtatttcctt atcggtatct gggggactga aagaaggatc tatgcagcaa taaaattttt
    14521 catctacacc gcctttggaa gcgcactgat gttcattgca attttatacc tgcacaaact
    14581 gcacatggat cagtttggca ttgccacaat gaacatcttt gatttctata acctgtccat
    14641 accttatgca ggaattctaa gtatgcaggg tctgctattt ctcgcatttt tcctggcttt
    14701 tgctatcaag gtcccaatgt tcccattcca cacatggctt ccagatgctc atgtcgaggc
    14761 gcctactgcg ggaagcgtaa tactggctgc ggttttactt aagatgggta catatggatt
    14821 tttaagattt ctcatgcctc tatttcctga ggccacgatt gaccttcttc cgatactctg
    14881 ctcaatagca gtgataggag ttatttacgg agcgcttgtt gcgtttgctc aaaaagactt
    14941 aaagaaactg gttgcttatt caagcgtaag tcacttggga ctggtcatgc tcggagtgct
    15001 cgtgctaaac atacagggtg ttcagggcgg catatatcag atgctgaacc acgggctttc
    15061 taccggcgca ttattcctta ttgtaggcat ggtatatgag aggcggcaca ccaaaaaaat
    15121 tgcggagttt ggcgggatcg caaaggtgat gcccatactg gcgctcttct ttatgcttgc
    15181 aacgctctcg tcaatcgggc ttccgctact aaacggtttt gtgggtgagt ttcttatact
    15241 tctcggtgca ttcaagtggg acgggaggtt ttcagttctc ggcacaacgg gcattgtact
    15301 tggggcaata tatatgctct gggcgtatca gcgcgtgatg ttcggcccat taaataagcc
    15361 ggagaacaag gcgctcaggg atttgagtgt tagagaaata tttgtcctta ctccgattgc
    15421 catcatgttc tttgttatgg gaatctatcc aaagccgttc ctggagagaa tcgagccttc
    15481 tgtaaacagc ctcattcaca ccaagtttgc tgagaccata aaacaggatg attccgaaaa
    15541 tgtcctatcg cagtatttta gagggcggta ggggtattta acaatccatc caaaaaagtc
    15601 tgtcatgctg agcgcatgcg aagcatctct cttttgattc tacttagatt cttcggctac
    15661 tatgattttt aaaaacttta aggtcttacg aactttacct taacggtaaa gcccgtcctc
    15721 aagtatgaca aaacaaaaca cactgagcat ttataagctt ctgttattac tctctttttt
    15781 catcttttac atatgaaaaa gtattatcag acaaaagcac tatttctgga aaaactattg
    15841 attttcatta cttaaaagca gtataatttt ctaggttaac atagaatcta tatgggggtg
    15901 aggtcatgtc ttattctgtt gaaggacagc tgctggaagt gtgtatttgt aatgttattt
    15961 gtccctgttg ggtgggagga gatcccgacg agggctactg tgatggtatg attggctggc
    16021 acatagatca aggcgaaatt gaaggagtga atgtcagcgg gtgtaatttc gcaatgttgc
    16081 tgcatagccc gggtaatgtt cttgatggta actggaaagt acggttatat gtggatgagg
    16141 aattatctga tgaacagcag gaaggaatat taaacctgtg gggaggtaag ctgggaggtg
    16201 caatcacgga tttagcatcc ctggtgggag aagttgcctc ggttgaaaaa acacatatca
    16261 ggtttgatgt agaaggagta aacgggacaa tagcgatagg agatgttgtc tcggcgtctc
    16321 ttgaagcttt taaaggagca acaggagcag aaactacact ccacaattcc gttttcacct
    16381 ctattccagg aaaacctgca
//
LOCUS       DBNGSV010000215         1348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_591709, whole genome shotgun sequence.
ACCESSION   DBNGSV010000215 DBNGSV010000000
VERSION     DBNGSV010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_591709"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1250
                     /locus_tag="AC159U_07830"
     CDS             join(109..370,370..1250)
                     /locus_tag="AC159U_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611339.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3337006.1"
                     /translation="MGRKRYSAEQIIRMLRQAEILSSQGRSVPEICRELGVTSNTYYR
                     WRKEYGGMGVSQAKRLKELEKENSRLKRAVADLTLDNLILKEAFKGKLLSTERRRRCV
                     VHVQQKLVVSERRACRVVGQARSVQRHKSNKSQSEEVLRSDIVRLASKYGRYGYRRIT
                     ALLKSEGWVVNHKRVERIWRQEGLKVPKKQPKKGRLYLNDGSCIRLRPLYKNHVWSYD
                     FIHDRLHSGKKIRMLTVIDEYTRKCLAIRVGFSLKSEDVLDVLSTLFITEGVPEYIRS
                     DNGSEFTAKTTREWIEAIGVKTAYIEPGSPWENGYNESFNGKLRDEFLNTEIFYTLKE
                     AQVLIEQWRRHYNEVRPHSSLGYRPPAPRAIVPEDKNRENYTQFVT"
BASE COUNT          491 a          230 c          314 g          313 t
ORIGIN      
        1 atttaggtaa ttgtttttgt ggagcagtac taacctgccc ccctataata ggaccatgtg
       61 ttaagttatt tttttgacat aaaaccctat aaataggagg tagtttgaat gggcaggaaa
      121 cgctatagtg cagagcagat aatacggatg ctaaggcagg cagaaatact ctcatcccag
      181 ggaagaagcg ttccagagat atgcagagag ctgggtgtta catctaatac ttactataga
      241 tggcgtaaag agtacggagg aatgggcgta agccaggcaa aacgcctaaa agagcttgag
      301 aaggaaaact caaggctaaa gagagcagta gctgatttaa cactagacaa cctaatatta
      361 aaggaagcct caaagggaaa cttctaagca ccgaacgaag acgaagatgt gtagttcatg
      421 tacaacagaa acttgtagta tcagagcgta gagcatgcag agtagtagga caagcacgtt
      481 cggtgcaaag acataaatct aataaatcac aatcagaaga agtccttaga tctgatatag
      541 tacgtcttgc aagtaagtat ggcagatatg ggtacagacg tattacagct cttcttaaat
      601 ctgaaggatg ggtagttaat cacaagcgtg tagagaggat atggagacag gaagggctta
      661 aagtacccaa gaaacagcca aaaaagggaa gactatactt aaacgatggt tcctgtataa
      721 gactacgccc tttatataaa aaccacgtat ggagctacga cttcatacac gacagactac
      781 acagtgggaa gaagataaga atgcttacag taatagatga atatacaagg aagtgtttag
      841 caataagagt tgggttctca ctaaaatcag aggatgtatt agatgtacta agcactctgt
      901 ttattacaga aggagttcca gagtacatac gctcagataa cggaagtgag tttactgcca
      961 aaaccactag agaatggata gaagcaattg gagtaaagac agcatatata gaaccaggct
     1021 caccctggga gaatggatac aatgagagtt ttaatggaaa gctaagggat gagtttctta
     1081 acacagagat attctacaca ctaaaggaag ctcaagttct aatagagcag tggagaaggc
     1141 actacaatga agtaagacca cacagttccc ttggatacag accccctgct ccaagagcta
     1201 tagtaccgga ggataaaaac agagaaaatt atactcaatt cgtaacttaa aaggtggacc
     1261 tactttcggg ggcttgacac tcctaatctc agtatgttga gactgaagac tcagaaatac
     1321 tatatatgga ttggtcacat ttgcataa
//
LOCUS       DBNGSV010000216         7243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_522972, whole genome shotgun sequence.
ACCESSION   DBNGSV010000216 DBNGSV010000000
VERSION     DBNGSV010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_522972"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /gene="pheT"
                     /locus_tag="AC159U_07835"
     CDS             complement(<1..1673)
                     /gene="pheT"
                     /locus_tag="AC159U_07835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3337007.1"
                     /translation="MLITLNWLKDFIDIKEDPYDLAEILTNAGLEVESVTPLASGLDN
                     IVVAKILKIRAHPDASRLVICDVTDGSTDYNIVCGAKNMKDGDIVALAKIGAKLPKTA
                     KFPDGIKIKKSKIRGEVSEGMLCAENEIGLGEDSEGIMILTDENPSLGSTIVEALGLD
                     DWVFEIGVTPNRPDCLSVLGVARQLSAVVNRKIKYPENSYEVFDIKENSNLRVEIKDE
                     DRCPRYTGRYIDNLEIRKSPWWLRQRLQACGVRAINNVVDITNYVMLEYGHPLHAFDA
                     KFLEGLKVIVRTAKQDEKINTLDKNERELTPDDLLICDASKPIAIAGVMGGENSEVSD
                     STTSLVLECAYFDPTTVRKTSKRLKLSTESSYRFERGLDPQSIDMVVDRTSYLLSALA
                     SGRVNKAIVDVYPKKAKPILINLNTQNLNSAVGINLSNSEIESILNSMDVEIEGSDGN
                     IVNCRVPTYRVDLTREIDLIEDIAIIYGYNSVPFTLPQVSVKSDINTKGNFSSVREIK
                     KFLAGSGFSEVINYSFSDPGALQMFSKKAGIDLVNPLTTEQSCMRTSII"
     gene            1969..3237
                     /locus_tag="AC159U_07840"
     CDS             1969..3237
                     /locus_tag="AC159U_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3337008.1"
                     /translation="MKILTKTTISFLLILLISSNIDSFAKNKVRTEIDSISAIQNIIF
                     DTAAGKIIVSIPEDILAGDMISGTITKEPKGKNESDMMDNLSKLDKYSVEINNETTVV
                     SERDIKFNIPKSSTGGVTYMILRGASGDEWARNYITYQNSSLNIKSYEAPSPWEYQSP
                     KIGRSSNPSVIKGPFDGNFGTTSIVIGNTSVKKIAESPRVLVFENPSNPIGNIDLVLN
                     ERGVNVKRKYSNIKVVKVDENGSSELTATGIMETDKNKQITKEAETPIEQDDSVVKVN
                     TDRKGDTSAMNEIVSENERGFLKETSIPIESGNEIINAKADPQSKEENTNNAAGIDNN
                     PKSSSTQSGKYTVQIASYKQQEDAMKLAKRLQSKGYEAYIIQAKVPSKGTWHRVRIGS
                     YPTKTEAKNYGSHLAKNEPLIDSMFVTLNN"
     gene            3431..3901
                     /locus_tag="AC159U_07845"
     CDS             3431..3901
                     /locus_tag="AC159U_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337009.1"
                     /translation="METIGKILSLVFIAVVGAGGATMYILDSNKPVVEQREVFDADKY
                     LGKADDDLAKYREHLRNSYIESQPPGEQVSTVKANQNVWSDTYTNKLPDFISEDEVKT
                     IASNHEVNELREKMNFWHIKYHKSLKNKKTLEANEAYKKHKSYKFALDYKNTFN"
     gene            complement(3987..4748)
                     /locus_tag="AC159U_07850"
     CDS             complement(3987..4748)
                     /locus_tag="AC159U_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3337010.1"
                     /translation="MNNKNTAIIFLNDLGDLTKLLYKAIIYSFKPPIEVKLILEQIYQ
                     LGFKSIPIVILSSVSIGMVMVVQMAYGFAAFGAKGLVGPLVTLAMVRELGPILSSLLV
                     GGRVGSGITAEIGSMNVTEQLDAMKTLGADPIKKLVVPRLIACIISFPLLAVIADLAG
                     IGGAMLIAYTELSVTPRLFLSEIVGFVLLADFLSGIVKTVFFGLIVAIIACYIGMKAS
                     GGTQGVGNSTTKTVVVSLVLIIIADFFLTKVFLDL"
     gene            complement(4738..5241)
                     /locus_tag="AC159U_07855"
     CDS             complement(4738..5241)
                     /locus_tag="AC159U_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337011.1"
                     /translation="MISCSSVDFIKAYSSLETKKNFTDQTFIKRDITYKVGKLNENWK
                     IVNLEFGDLAFYNKSLNSTINVNSTCSNTGNYNLTTLSDSLLSGLKNKQLIERLVATA
                     NKEGALESKYYGQYDNNRVKMSIIVYQKELCVYDFSYISDVLNFNKGYKEFKNFISEF
                     ELIENEQ"
     gene            5421..5678
                     /locus_tag="AC159U_07860"
     CDS             5421..5678
                     /locus_tag="AC159U_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR3337012.1"
                     /translation="MNKITVYTSSFCGYCIAAKSLLSKKGVPFEEINLSTSPELREDV
                     VEKWQWRTLPLIIINDNLIGGFRELAMLESSNKLDSLINSD"
     gene            5718..7127
                     /gene="gltX"
                     /locus_tag="AC159U_07865"
     CDS             5718..7127
                     /gene="gltX"
                     /locus_tag="AC159U_07865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3337013.1"
                     /translation="MSVITRFAPSPTGALHIGGVRTAIFNWLYARHNKGKFLLRIEDT
                     DQQRSTEESVTEILEGLKWLGLDWDGNPVRQSSNLDKYNEFANKLIEQGDAYRCYCTP
                     EEIEQRRAEFKEKNITYRYEGTCRNNDNKYSDKSYAVRLKTPENGFIEIKDKLRGTIS
                     YDLKEIDDFVIIKRDGFPTYNFAAVIDDSSMGITDIIRGDDHLSNTPKQALIYNMLGL
                     KLPNFAHVSMILGQDKAKLSKRHGAVSILAYRDNGYLPESMLNFLVRLGWSHGDQEIF
                     SKEQLIELFNLESLGKTPAVFNEEKLSWLNTHYIKNTDIENLARHTAPFFEKSGIDVT
                     QNRDKFHGVLNTLRERVQTLDEYPEKSIYFFKDVNVYEEKADKKFLNDKTLPILQKIK
                     DELSAVEIFEHEILHSTFEKIMADLDLKFGKIAQPLRVALTGGTVSPGIFEVIETLGK
                     ETTLMRLDNAINHIQSNNQ"
BASE COUNT         2550 a         1324 c         1220 g         2149 t
ORIGIN      
        1 tgaataatgg aagttctcat acaagactgc tctgtagtaa gcggatttac taaatctatt
       61 cctgcttttt ttgaaaacat ctgcagtgca cccggatcag aaaaactgta atttatcact
      121 tcactaaatc ctgaaccggc aagaaatttc tttatctccc ttactgagct aaaattgcct
      181 ttagtgttta tatcgctttt tactgaaacc tgaggcagcg tgaaaggcac agagttataa
      241 ccataaatta ttgcaatatc ctcaatgaga tcaatctccc ttgtgagatc caccctgtat
      301 gttggcaccc tgcaattaac aatgtttcca tccgagcctt caatttctac atccatagag
      361 tttaatatgc tctcaatttc actgtttgag agattaatcc caactgctga attgaggttt
      421 tgtgtgttta ggtttattaa tataggttta gcttttttag gataaacatc tacgatggct
      481 ttattaaccc tacccgacgc taatgcactg agcagatagc ttgtcctatc taccaccatg
      541 tctattgatt gagggtcaag acctctttca aacctgtaag atgactccgt agaaagcttt
      601 aatctctttg atgtctttct aacagttgtt ggatcaaaat atgcacactc aagtactaaa
      661 gaggtagtgc tgtcagacac ttcagaattt tctccaccca taacccccgc aatagcaatc
      721 ggctttgaag catcacaaat aagtaaatca tccggtgtca gttctctttc gtttttatca
      781 agggtgttaa tcttttcatc ctgttttgca gttcttacta taaccttcaa tccttccaga
      841 aacttagcat caaacgcatg taaaggatga ccatattcaa gcatcacata gtttgtaata
      901 tcgactacat tattgatggc cctaactccg caggcctgca gcctttgcct aagccaccag
      961 ggtgatttac gtatttcgag attatctata tatcttcccg tgtatcttgg gcaccggtct
     1021 tcgtctttta tctcgactct aagattacta ttttctttaa tatcaaaaac ttcataacta
     1081 ttttcagggt attttatttt tctgttaacc acagccgaga gctgtcttgc cacacccaat
     1141 acactaaggc agtcgggtct gttgggggtt accccaatct caaacaccca gtcatcaagt
     1201 cccagcgctt ctacaatagt gcttccaaga gaaggatttt catcagttaa gatcataatt
     1261 ccttcgctat cttctccaag accaatctca ttttctgcac acagcatacc ttcagatact
     1321 tcgcccctga ttttagattt cttgattttt attccgtccg gaaactttgc agtttttgga
     1381 agttttgctc caatcttggc aagcgcaaca atgtctccat ctttcatatt ttttgcgcca
     1441 catacgatat tatagtctgt gctaccgtct gttacgtcac atataacaag cctggatgca
     1501 tcgggatgcg ccctaatctt aagtatctta gccactacga tgttatccag tcctgaagcc
     1561 agcggagtta cagactctac ctcaagacca gcatttgtga gaatttcggc aagatcataa
     1621 ggatcttctt taatatcaat aaagtcttta agccagttaa gtgtaataag catattgtta
     1681 taatttataa actaaattta ctatctctat ttaatcttaa tcaatgataa taaaccttcg
     1741 ttctacaaaa taatcaaatc cagagtgata ttgttttact aaaatatcca gacaaagcaa
     1801 acaacgactt ttttaattat aaaaaaattt atatccataa aagattataa tttaagaatt
     1861 tactgatata tcactttaag tataatatat aacctattga tttatatatt tattttagtc
     1921 ataatatata cgaatcaaat ccaaattata ttttaaaaat ttaaaaatat gaaaatatta
     1981 acaaaaacaa caatttcttt tctgttaatt ttgcttataa gctcaaatat agatagtttt
     2041 gcaaaaaata aggtaaggac cgaaatagac tctatatcgg caattcagaa tattatcttt
     2101 gatactgctg cgggcaagat catagtatcc atacctgaag atatactcgc aggagatatg
     2161 ataagcggta cgataacaaa agaacctaaa ggaaaaaatg aaagcgacat gatggataat
     2221 ttatctaaac tggataaata ttctgtagag attaacaatg aaacaacggt tgtaagcgaa
     2281 agagatataa aatttaatat tccaaaatca agcaccggcg gagtaactta catgatactc
     2341 agaggagcca gcggagatga atgggcaaga aattatatta cttaccaaaa ttcttctcta
     2401 aatattaaga gctatgaagc gccttctcca tgggaatatc aatcaccaaa gattggcaga
     2461 tcatcaaatc catcggttat aaaaggaccg tttgatggaa attttggcac aaccagcatt
     2521 gtaattggca atacatctgt aaagaaaatt gccgaatcac caagagtgtt ggtgtttgaa
     2581 aatccttcaa atcctatagg caatatagat ctggttttaa atgaacgcgg ggtaaatgtt
     2641 aagagaaaat acagcaatat caaagttgtt aaagttgatg aaaacggaag tagtgaacta
     2701 acagctacag gaattatgga aacagataag aataaacaaa taacaaaaga agctgaaaca
     2761 ccaatagaac aagatgacag tgttgtaaaa gtaaacactg atagaaaagg tgatacctcc
     2821 gcaatgaatg aaatcgtatc cgaaaatgag cggggatttc ttaaagaaac aagcattcca
     2881 atagaatctg gcaatgaaat tattaatgca aaagcagatc ctcaaagcaa agaagaaaat
     2941 acaaataatg ctgcagggat tgacaataat ccaaaaagtt catcaaccca atcaggcaaa
     3001 tacacagtac agattgcttc atataaacag caagaagatg caatgaagct tgcaaagaga
     3061 ctacagtcaa aaggatatga agcttatata attcaggcaa aggtaccttc taaaggcaca
     3121 tggcacaggg taaggatagg aagctatcca accaaaacag aggctaaaaa ttacggcagc
     3181 catttagcta aaaatgaacc ccttatagac tcaatgttcg ttactctaaa taattagaat
     3241 tgataggata tccaaaatac ccgtcatacc actacatatt gtatatataa agaaataata
     3301 aatacaatat attgtgttca ctgtttattt taaaaatttg taaaataagt ctttacaaac
     3361 atatcaaaaa gctataaaat actacaacta aagtaataga ttcaataata aggattattg
     3421 gagaatagag atggagacta ttggaaaaat tttaagtctt gttttcatag cggttgtggg
     3481 tgccggtgga gcaacgatgt atatacttga ttccaacaaa ccggtggtag aacaaagaga
     3541 ggtattcgat gctgataaat accttggaaa agcagatgat gatctggcaa agtacaggga
     3601 gcatttaaga aacagttata tcgaaagcca gccacctggt gaacaggttt ctactgtaaa
     3661 agccaatcag aacgtctgga gtgatacata cacaaataag cttcctgact tcatatccga
     3721 agatgaagtt aagactatcg catcgaatca tgaagttaat gagcttcgcg agaagatgaa
     3781 cttctggcat atcaagtatc acaaatcgct taaaaacaaa aagacattag aagctaatga
     3841 agcttataaa aagcataaaa gctataagtt tgccttagat tacaagaata cttttaatta
     3901 aactgattta ctaaattttt taaagcgaag ccagactata tctatatgat gtagtctggc
     3961 tttttttatt cctttaatgt gttatatcat aaatccagaa ataccttggt cagaaaaaaa
     4021 tcagcgataa taataagcac caaagacact accaccgtct ttgtagtgga atttccaact
     4081 ccctgagtac cgcctgaagc cttcattcct atataacatg ctattatcgc tacgataagt
     4141 ccaaagaata cagttttaac aatcccgctc aaaaagtcag ccaacaatac aaaacccaca
     4201 atctcggata aaaaaagtct aggtgtaaca ctaagctcgg tataggcaat gagcatggct
     4261 cctccaatac ctgcaagatc cgctataaca gcaaggagcg gaaatgatat aatgcatgca
     4321 ataagtcttg gaaccacaag ttttttaata ggatcagctc caagagtctt catagcgtcc
     4381 agttgttcag ttacattcat agatccgatc tctgccgtaa taccagagcc aacccttcca
     4441 cccacaagta gtgaagacag aatagggcca agctctctta ccattgcaag tgtaaccaaa
     4501 ggtccaacaa gccctttggc tccaaaagct gcaaacccgt atgccatctg aactaccata
     4561 accataccta ttgaaactga tgagaggatt actattggaa tagacttaaa acccagctga
     4621 tatatctgtt ccagaattaa ttttacttca attggaggtt taaaagaata tattattgcc
     4681 ttatataaga gtttagtcag atcccccaga tcatttaaga aaattatagc tgtattttta
     4741 ttgttcattt tctataagtt caaattcgga aataaagttt ttaaattcct tatagccttt
     4801 attaaaattc agcacgtctg agatgtacga aaagtcataa acacatagct ccttttggta
     4861 tactataata ctcatcttta ctctattatt atcatattgg ccataatact tactctcaag
     4921 cgccccttct ttattagccg tagctactaa cctttcaatt aattgcttat tttttaaccc
     4981 tgatagtagt gaatcggata aagttgttag gttataattg ccagtgttgc tgcacgtaga
     5041 gttaacatta atagtcgagt ttagtgattt attatagaag gctagatctc caaattccag
     5101 atttacaatc ttccagtttt catttaattt tcctacctta tatgttatat ctctttttat
     5161 aaatgtctgg tcagtaaagt tttttttggt ctccagtgat gagtaagcct taataaagtc
     5221 tacactggaa cacgaaatca ttaacaataa aaaactcagt agtaatttaa aacaattttg
     5281 tatcatttat aatttgttta tttagtaata taaaaatatc acagcaattt tatataagca
     5341 cattaaataa tatcttataa aataatagaa tccatctttt ggcagcactc atcataatta
     5401 atattttaca aattggctaa atgaataaaa taacagtata tacttctagc ttttgcgggt
     5461 actgcattgc agcaaagtct ctgcttagca aaaaaggtgt gccgtttgaa gaaattaatc
     5521 tatcaacttc acctgaactt agagaagatg tagtagaaaa atggcaatgg cgcactttac
     5581 ctctaataat tattaatgat aacctcattg gaggcttcag ggaacttgca atgcttgaga
     5641 gcagtaataa actggatagt ttgataaatt cagattaaac tcctttcttt ttttaaacta
     5701 aaatcaagga taacaatatg agtgtaatta cgagatttgc cccaagcccc acaggagctt
     5761 tgcatattgg tggagtaagg accgctatat ttaactggct ttatgcaaga cataataaag
     5821 gcaaattttt gcttagaatt gaagataccg accagcaaag atcaaccgag gagtctgtaa
     5881 ctgaaatttt ggaaggactt aaatggcttg ggctggactg ggacggcaac cccgtaagac
     5941 agtcatcaaa cctggacaaa tataacgagt ttgcaaacaa actcatagaa caaggggatg
     6001 cttataggtg ttactgtact cctgaagaga tagaacaaag aagagctgaa tttaaagaaa
     6061 aaaatattac ttacagatat gaaggtactt gcagaaataa tgacaacaaa tactcggata
     6121 agtcttatgc agtccgcctc aaaacccctg aaaatggttt tattgaaata aaagataaac
     6181 ttaggggaac aataagctac gacctaaaag aaatagacga ttttgtgatt ataaaacgtg
     6241 atggttttcc cacctataac tttgctgctg ttatcgatga ctcctccatg ggaataaccg
     6301 atataataag aggagacgac catctatcaa acacacccaa acaagctctg atatataata
     6361 tgcttggact taaactacca aactttgccc atgtctccat gatcctgggg caggacaagg
     6421 caaagctaag caagaggcat ggagccgtat ccattttagc ttacagagat aacggctatt
     6481 tgccagaatc catgctcaat tttttagtgc ggcttggctg gtctcatggt gatcaggaaa
     6541 tattttctaa agagcagtta atagaactct ttaatttaga gagtcttggc aaaactcctg
     6601 ctgtatttaa cgaagaaaaa ctctcgtggc taaatactca ttatattaag aacacagata
     6661 tagaaaattt agcaagacac acagccccat tttttgaaaa aagcggaata gatgtaaccc
     6721 aaaacaggga taagtttcat ggtgtattaa atactcttag agaaagagtt caaactctgg
     6781 atgaatatcc ggaaaaaagc atttattttt tcaaagatgt gaatgtttat gaggaaaaag
     6841 cggataaaaa gtttctaaat gataaaacac ttcccatatt acaaaaaata aaggatgagt
     6901 tatctgctgt ggaaattttt gaacacgaga ttcttcacag cacatttgaa aaaatcatgg
     6961 ctgaccttga tcttaaattc ggcaagattg cacagcccct aagagtagcg cttacaggag
     7021 gaactgtaag ccccggaata tttgaggtaa tagaaacact gggaaaggaa actacactaa
     7081 tgaggcttga taatgcaata aatcacatcc aatcaaataa ccagtaatcc atgtaaagtt
     7141 catataacac ctatccgatt atcttttatt aaccagggat aaggtattta tttatctcat
     7201 attttgattt attgcctagt ggtcttagaa tataagagat gaa
//
LOCUS       DBNGSV010000217         8728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_939034, whole genome shotgun sequence.
ACCESSION   DBNGSV010000217 DBNGSV010000000
VERSION     DBNGSV010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_939034"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..630
                     /locus_tag="AC159U_07870"
     CDS             31..630
                     /locus_tag="AC159U_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3337014.1"
                     /translation="MNNKILILFSHPAIQKSRVNKGLIESVKNLDGITFHDLYEKYPD
                     FLINVTVEQNLLKEHDIIVFHHPFYWYGCPAILKEWMDLVLEYGFAYGPEGNKLEGKK
                     IISVITTGGSKDAYKPDGHNKYTIRQFLLPFEQTASLCRMQYLPPLVIHDSISMNLNM
                     QLHQYAELYKNILIMLRDDKINYTKALEFEYINDYYKNI"
     gene            639..2516
                     /locus_tag="AC159U_07875"
     CDS             639..2516
                     /locus_tag="AC159U_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIR3337015.1"
                     /translation="MHVDSFLFQTFVYLIAAVISVPLAKRLGLGSVLGYLIAGVIIGP
                     FVLGFVGSESEGVMHFAELGVVLMLFLIGLELEPSLLWRLKNSILGLGSLQVFITTAL
                     ISLITIVVGYSWKEALAIGLILSLSSTAIVLQTLNEKGLLNSQGGRFSFSVLLFQDIS
                     LIPILAFLPLLALSVDPGELSLPHSGSESFVESLKSWQRGVLIIAIVSSIILGGRFLS
                     RPVFRFIAETRLREIFTAFSLLLIIATALAMQFVGLSPALGTFVAGVVLANSEYRHEL
                     ESDIEPFKGLLLGLFFISVGASINFNLLFGNFTIILGIVFLLMALKFVVLFILGRVFK
                     INKTENYLFAFALAQGGEFAFVLFSFATQNSVLPETVSSLLVVVVAITMMLTPLLFIF
                     YEKFIEKRFQESEEKEPDEIDEKNQVIIAGFGRFGQIIGRLLHGHEISTTVLDHDASQ
                     IELVRKFGYKVFYGDASRVDLLKSAGCEQAKLFIIAVDDKEKTLQIAREIKSNFPHLK
                     ILARSFDRRHTYDLMKLEVDYIKRETFYSALELGIDALMQLGFPAYQARRAALTFKHH
                     DKQTLIDLYEHFGDEKNFIYHSKRRTEDLLNLLMEDEKDIKQADDDLSWERPEDPAEK
                     N"
     gene            2537..3811
                     /locus_tag="AC159U_07880"
     CDS             2537..3811
                     /locus_tag="AC159U_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3337016.1"
                     /translation="MHKTSEKNIYNKKIISWCMYDWANSAFSTTVMAAILPVYYSKVA
                     GSTLEGNTATIYWGYTTAIALLITAFIAPIMGAIADFSGAKKKFLMIFASVGIFFTTL
                     LYLITTGDWFMASLFFVFGNVGFATSEVFYNSLLPEVAKSEDIDRVSTRGYALGYLGG
                     GILLAINVVMLNIIDDKEFASRLCFVTVGIWWAVFTIPLIQNIEEPPRKNRASNKFNP
                     LYQGFRRLRKTFGELKRYRQLLIFLAAFWIYNDGIGTIIKMATIYGSEIGIGTNDLIG
                     ALLMTQFVGIPFAILFGRLAGRIGAKKSIYLGLFVYTLICIGAYFMTKGIHFWMLAFC
                     VALVQGGTQALSRSLFASMLPKAKTAEFFGFYGMSSKFAGIFGPLIFAMVGQLTGTSR
                     LSIISLVVFFIIGAVVLSRVDEKEGIRMAQNN"
     gene            3880..4158
                     /locus_tag="AC159U_07885"
     CDS             3880..4158
                     /locus_tag="AC159U_07885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3337017.1"
                     /translation="MASASARHILVNSEEQCNELKGKIEGGEDFGDIAKEHSLCPSGK
                     EGGNLGEFNQGMMVPEFDMVVFTAPLNQVHGPVKTQFGFHLIEILSRS"
     gene            4179..5060
                     /locus_tag="AC159U_07890"
     CDS             4179..5060
                     /locus_tag="AC159U_07890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3337018.1"
                     /translation="MEVFEAMGTRRSARFFKPWKEVEDWKIQKMLQAARYSSCQGNCS
                     STEAIVIDKKTYPEEKFEQIVECASTFNEIHIRQAPIVIAWLINLDAWYKELVKSFAV
                     LFPSRAITAAHGWTYKQLTEQTYPRLMSFPKDKTEDLLRVEAGQAIANSMLAATDMGL
                     SCCLLATGRKPAEFPKVLGTPENIVPIWLMAVGYAAESPGQRPRKRFDRLYHYNEYGT
                     PLKEDAGVRQELVDQKLIQPMDPLPGREEELKHICRMFGFTEDMPDMPKDHIKAMYEE
                     DSPYYGELPPDLEKKGV"
     gene            5060..5692
                     /locus_tag="AC159U_07895"
     CDS             5060..5692
                     /locus_tag="AC159U_07895"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004409690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3337019.1"
                     /translation="MHCPLCKSKEISDYFSNKRRDFLQCLKCYLVFVPERFHLSAEEE
                     KKRYDLHENNPEDGGYRKFLSRLAIPLNEKLKSNSHGLDFGSGPGPTLSNMLEEYGYT
                     VSLYDCFYADDKGVLKKKYDFITATEVFEHLRNPIETIELLWQRLKPGGIFGIMTKLV
                     IDKEAFSTWHYITDRTHICFFSVKTFKWLANKLEAKIEFIGNDVIILYKN"
     gene            5704..6975
                     /gene="mltA"
                     /locus_tag="AC159U_07900"
     CDS             5704..6975
                     /gene="mltA"
                     /locus_tag="AC159U_07900"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR3337020.1"
                     /translation="MKILLAIVLSVLVFTSCARESIKTPKQAMRLTQNLPELTDDLPL
                     SDLEQALSQKIERLKEIDNERLIFGPKSVKKDDYIFALKFLEVKLRSGIAKEQFNDLV
                     NNNFDFYEVYGKEKWGEVLITSYYEPVLDGSTVKSGGFTQPLYSVPDDFVTIHMGKFV
                     DTFDKLSPIKDEVLDSHNNIKVLRGRVSQPYVEGGSQNVVPYYTRKEIDSEGKLSGRG
                     LELAWVDPIDAFFLHVQGSGTVLFDDGTKLRLGYASKNGYPFYAIGKHLLDTIPKEKM
                     SMQSIEQYLRSLPESEMRDILNLNKSYVFFRELETRPITSFGTEVVDGRTIATDKRFF
                     PMGTLAFLQFEKPVFDGPESRHPSGWKKASRFVLDHDTGGAIKGTHRVDLFWGSGKEA
                     GQSAGVMKQWGKLFYLVPKDSLINKFKSAGN"
     gene            complement(6978..7640)
                     /locus_tag="AC159U_07905"
     CDS             complement(6978..7640)
                     /locus_tag="AC159U_07905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005177532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3337021.1"
                     /translation="MELYEVMHTTFSAREYTDDPVTDEVIYKVLDNSRFAPSGGNRQG
                     WKVIVIRNQESKEKIAELAVPGAKKYAAQRIAGENPWNTIDPSGVTEQDIVNTPPSPK
                     FHMTYVDAPVILIVCVDLKVVAAMDQNLDRMGLVSGASIYPFVWNILLSARNEGLGGR
                     ITTIPISNEPAIQDYLNIPRHIAIAAIIPMGKPVKQITKLNRKPVEDFTYLEKWDGDR
                     LR"
     gene            7758..8285
                     /locus_tag="AC159U_07910"
     CDS             7758..8285
                     /locus_tag="AC159U_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3337022.1"
                     /translation="MRKVLVLIAVFMVVGCTQYAQKEAAKPMTDVEKAGQLWAMMQKE
                     DYRNTWKMWPGKEAFYKGAHPHGALLTTYVSESAYDALVNKKGSMPDGAIIIKENYMP
                     DKKLGAITVMKKKAGYNPEAGDYFWVKFGGDGSVLTKDMEDGSKLDLAGKVPGCIGCH
                     TAVKANDFLFTGPIK"
BASE COUNT         2685 a         1490 c         1893 g         2660 t
ORIGIN      
        1 tactcggttg agatataatt gtaataataa atgaataaca aaatattaat cctcttttcc
       61 catccggcta tacagaagtc cagagttaat aaagggctta tcgagtctgt taagaactta
      121 gatggtatta cattccacga cctgtatgag aagtatcctg attttcttat taatgttaca
      181 gtagaacaaa acctcctaaa ggagcacgat attattgttt ttcatcatcc gttttactgg
      241 tacggatgcc ccgcgatatt aaaggagtgg atggatcttg tgcttgaata cggttttgct
      301 tacgggccgg aaggaaacaa actggaaggc aaaaaaataa ttagtgtaat tacaacaggt
      361 ggtagcaagg atgcctataa accagatggg cacaataagt atacaattcg gcagttcctg
      421 ctgccgtttg agcagactgc aagtctttgc cgtatgcagt acctgcctcc gcttgttatt
      481 catgattcta ttagtatgaa tttaaatatg cagctccatc agtatgcaga gctctacaaa
      541 aatatattga taatgctaag agacgataaa ataaattata ctaaggccct tgagtttgaa
      601 tatataaacg actactacaa aaacatttaa cttaaactat gcatgtagac agctttttat
      661 ttcagacatt tgtatatctt attgcagccg taatttctgt acccctggca aagagactag
      721 ggcttgggtc tgtccttggg tatttgatag caggggtaat catagggccg tttgtacttg
      781 gatttgtagg gagtgaaagt gaaggagtaa tgcattttgc agagcttggt gtagtgctca
      841 tgctttttct gataggtctt gagcttgaac cttcattgtt atggagacta aaaaattcaa
      901 ttctgggtct ggggtctctg caagttttta tcaccactgc attaatatcc ctgattacta
      961 ttgtagtagg atatagctgg aaagaagccc ttgctatcgg gcttatactt tcactatcat
     1021 ccacagcaat agtgctgcaa acattaaatg aaaaaggact gttaaattcg cagggtgggc
     1081 gctttagttt ttcagttcta ctgtttcagg atatatcact tatacccata cttgccttct
     1141 taccacttct tgcattatct gtagacccgg gtgagttgtc tttaccacat tctgggagtg
     1201 agagttttgt agaatctcta aagtcctggc aaagaggtgt actcatcatt gctatagtaa
     1261 gctcaataat attaggcggc cgttttttat ccagaccagt gtttaggttt attgcagaga
     1321 caaggcttag agaaatattc actgcttttt cactcctgct tatcatagca accgcacttg
     1381 caatgcagtt tgtgggtctt tcaccagcgc ttggaacatt cgtggctggt gtagtgcttg
     1441 caaacagtga gtatcgtcat gagcttgagt ctgacattga accatttaaa ggactcttat
     1501 tggggctctt tttcatatcg gttggagcaa gcatcaattt taatctgttg tttgggaatt
     1561 ttactattat tttggggata gtctttttgc ttatggcgct taagtttgta gtgcttttta
     1621 tacttggaag agtatttaag ataaataaga cagaaaacta cctttttgcc tttgcacttg
     1681 cacagggtgg agaatttgca tttgtactgt tttcatttgc aacccagaac agcgtgcttc
     1741 ctgagacggt ttccagcctt cttgtagtag tagtagctat tacgatgatg ctcacaccgc
     1801 tgctttttat attttatgaa aagttcatag aaaaaaggtt tcaggaatcg gaagaaaaag
     1861 agcctgatga aatagatgaa aaaaatcagg taataatcgc aggttttgga aggtttggtc
     1921 agataatagg caggctgctt catggtcatg agataagcac aaccgtgctt gaccatgatg
     1981 caagccagat agagctagta agaaaattcg gctacaaggt gttttacgga gatgcttcaa
     2041 gggtagattt actcaaatct gccggatgtg agcaggcaaa gctatttatt atagccgtag
     2101 acgataagga aaagacccta cagatagcaa gggagattaa aagcaatttt ccgcatctta
     2161 agatccttgc taggtctttt gacagaagac acacatatga tcttatgaag ttagaggtag
     2221 attatatcaa gagagagaca ttctattcag cccttgagct tggaatagac gctttaatgc
     2281 agcttggctt tcctgcatat caggccagac gtgcagcact tacttttaag caccatgaca
     2341 aacagacact gattgacctt tatgaacatt tcggagacga gaagaatttc atttatcatt
     2401 caaaacgcag aactgaggat ttattaaacc ttcttatgga agatgaaaag gatataaaac
     2461 aagcagatga tgacctttcg tgggaaagac cagaagatcc tgctgagaag aactaataaa
     2521 tatctctgat ttaaaaatgc ataaaacatc tgaaaaaaat atttacaata aaaaaatcat
     2581 aagctggtgt atgtatgact gggcaaattc tgcattctct actacggtta tggcagcaat
     2641 attgcctgtg tattatagca aggttgcagg gtctactcta gagggaaaca cagcaacaat
     2701 ttactgggga tatactactg ccattgcact gcttattaca gcattcatag cacctataat
     2761 gggcgcaata gcagattttt cgggggctaa aaaaaaattc ctgatgatct ttgcatcggt
     2821 tggaatattt tttaccacac ttctatacct tataacaacg ggtgactggt ttatggcatc
     2881 tctgtttttt gtttttggca atgtagggtt tgcaacctct gaggtgtttt acaactcgct
     2941 tttacccgaa gttgcgaaat cagaagatat tgacagagta tcaacccgcg gatatgcact
     3001 tggttacctg ggcgggggga tacttcttgc cattaacgta gtgatgttaa atatcatcga
     3061 tgataaggag tttgcatcga ggttatgttt tgtgactgtt ggtatatggt gggctgtttt
     3121 tacaattcct ttaatacaaa acatagaaga acctccccga aagaacaggg catctaacaa
     3181 attcaatccg ctgtatcaag gattcagaag acttagaaaa acatttggtg agctcaaacg
     3241 gtacagacag cttctcatat ttcttgcagc gttttggata tataacgacg gcatcggaac
     3301 gattattaag atggcaacga tctatggttc tgagattggt ataggtacaa atgatcttat
     3361 tggagcctta ctaatgactc agtttgttgg aattcctttt gccatattgt ttggcaggct
     3421 tgcaggtaga atcggtgcaa agaaaagcat atatctgggt ctgtttgttt ataccttaat
     3481 ttgtataggt gcctacttta tgacaaaagg tattcatttt tggatgctgg cattttgtgt
     3541 cgcacttgtc cagggaggaa cgcaggcgct aagcagatca ctttttgcat cgatgctccc
     3601 aaaggcaaaa acagcggagt tttttgggtt ctacggcatg agctcaaaat ttgcaggcat
     3661 attcggacct ctcatttttg ctatggtagg ccagcttact ggcacaagca gattaagcat
     3721 tatttcactt gtagtgtttt ttattatagg tgcagtagtg ttgagcagag tggatgaaaa
     3781 agaaggtatt agaatggcac agaataattg aacagattgc agaactttaa atataatgtt
     3841 aaaaatttta gagtaaatat ttatgtacag gagaaattaa tggcaagtgc aagtgcaaga
     3901 cacatattgg ttaatagtga agaacagtgc aatgaattaa aaggcaagat cgaaggtgga
     3961 gaggattttg gagatatagc aaaagagcat tcgttatgtc catctggaaa agagggcggg
     4021 aacctcgggg aatttaatca gggaatgatg gtacccgaat ttgatatggt cgtatttaca
     4081 gcaccattaa atcaagttca cggcccagta aaaacccagt ttggttttca tttaattgaa
     4141 attttaagta gaagttaatc cataccagga ggcttattat ggaagtattt gaagcaatgg
     4201 gtactagaag aagcgccagg ttttttaagc catggaaaga agtagaagac tggaagattc
     4261 aaaagatgct tcaggctgcg cgctattcgt cttgtcaggg aaattgcagc tctacagagg
     4321 ctattgtaat agataagaaa acatacccgg aggaaaaatt cgagcagatc gtggaatgtg
     4381 catcaacttt taatgagatt catatacggc aggcccctat tgtcatagcc tggcttatta
     4441 atttggatgc atggtataaa gagcttgtga agagctttgc agtattgttt ccttcaaggg
     4501 ctattacagc agcacacgga tggacatata aacagcttac agaacagact tatccaaggc
     4561 ttatgagctt tccaaaggat aaaacagaag acctgctgag ggttgaggca ggacaggcga
     4621 ttgcaaattc tatgcttgcg gcaacggata tgggtctttc atgctgcctg cttgcaacgg
     4681 gaagaaaacc agcagagttt ccaaaggtgc tgggtactcc tgaaaatata gttcctatct
     4741 ggcttatggc agtaggatat gccgcagagt cacccgggca gagaccaaga aaacgcttcg
     4801 acaggcttta tcactacaat gagtatggga ctccactcaa agaggatgct ggtgtaagac
     4861 aggagctagt agaccagaaa ttgatccagc ccatggaccc gctcccgggc agagaagagg
     4921 agctaaagca catctgcaga atgtttggat ttactgaaga tatgccagat atgccaaagg
     4981 atcacattaa agctatgtat gaagaagact ctccttacta tggcgaactt cccccggacc
     5041 ttgagaaaaa aggagtttaa tgcactgccc tttgtgtaaa agcaaagaga tttcggatta
     5101 cttcagtaat aagagaaggg attttttaca gtgtttaaaa tgttatctgg tatttgtacc
     5161 tgagaggttt catttatcag ctgaagaaga aaaaaaacgc tatgaccttc atgaaaataa
     5221 tccagaggat ggtggttata gaaaattttt aagcagacta gctataccac ttaatgagaa
     5281 attaaaatca aatagtcacg ggctagattt tggatcaggc ccggggccta cgctgtcaaa
     5341 catgctggaa gaatatggtt atactgtaag tctttatgac tgcttctatg cggatgataa
     5401 gggagtactc aaaaaaaaat atgatttcat tacggctacc gaagtattcg aacatttgag
     5461 aaatccaatt gaaactattg agttgctatg gcagagatta aaacccggcg gaatatttgg
     5521 tattatgaca aagctggtta tagataaaga agctttttct acctggcact atattacaga
     5581 tagaacccat atatgttttt tctccgttaa gacatttaag tggcttgcaa ataaattgga
     5641 agccaaaata gaatttattg gaaatgatgt aattatctta tataaaaatt aatattaatt
     5701 attatgaaaa tattattagc gattgtactt tctgttctgg tatttacatc ctgtgcaaga
     5761 gaaagcataa agactcctaa gcaggctatg cgacttacac aaaatcttcc tgagcttact
     5821 gatgatctac cactttcaga tttggaacaa gctttgtctc agaagattga acgtttgaaa
     5881 gaaatagata atgaacggtt gatattcgga cctaaatcag taaaaaaaga tgactatatt
     5941 tttgcactta agtttttgga agtgaagtta aggtctggaa tagcaaaaga acagtttaat
     6001 gatttagtaa ataataattt tgatttttat gaagtatacg gcaaggaaaa atggggagag
     6061 gttttaataa catcttatta tgaacctgtc cttgacggtt ctacagtaaa aagcggaggg
     6121 ttcactcaac ctctgtattc cgtaccggat gattttgtga ctattcacat gggaaagttt
     6181 gtagatacat ttgataaact ctcacccata aaagatgaag ttttagatag ccataacaat
     6241 ataaaagtgc ttagaggcag agtgtcccag ccttatgtag aggggggcag tcagaatgtg
     6301 gttccgtatt acacaagaaa agaaatagat tcagagggaa agctttccgg caggggactt
     6361 gagcttgcat gggtagatcc catcgatgca ttttttcttc atgttcaggg atctggaact
     6421 gtactttttg atgatggtac taagctcaga ctcggctatg catcaaaaaa cggttatccg
     6481 ttctatgcaa ttggaaaaca cttgctcgat acaattccaa aggaaaaaat gagtatgcag
     6541 tcaattgagc agtatcttcg cagccttccc gaaagcgaaa tgagggatat tcttaatctc
     6601 aataaaagct atgtgttttt tagagagctt gagacaagac caatcacatc ttttggcact
     6661 gaggtagtgg acggaaggac tatagctact gacaagaggt tttttccaat gggtacactt
     6721 gcatttttgc aatttgaaaa gcctgtattc gatggtccgg aatcaagaca cccatccgga
     6781 tggaaaaagg catcaagatt tgttcttgat catgacacag gaggagccat taaaggaacc
     6841 cacagggtcg atcttttttg gggaagcggc aaagaagccg gccagagtgc gggggtaatg
     6901 aagcagtggg gaaagttatt ttatctggtt ccaaaagata gtcttataaa caaatttaag
     6961 tctgctggaa actgatatta cctcaatcta tcaccgtccc atttttctag ataagtaaaa
     7021 tcttctacag gttttctgtt taatttggta atttgtttta caggttttcc cataggtatt
     7081 attgctgcaa ttgcaatgtg cctgggaata tttaaataat cctgaatcgc aggttcattt
     7141 gaaattggaa tagttgtaat tcttcctcct agtccttcat ttcttgctga taacaaaatg
     7201 ttccaaacaa atggataaat tgatgcaccg cttacaagcc ccatcctgtc caggttctga
     7261 tccatcgctg caactacttt taggtctaca cagacaataa gtatcacagg tgcatcaaca
     7321 taggtcatgt gaaatttagg tgaaggggga gtatttacta tgtcctgctc agttactcca
     7381 ctggggtcaa ttgtattcca tggattttct ccggctattc tttgtgccgc atatttcttt
     7441 gctcccggaa cagcaagctc ggctatcttt tctttcgact cctgatttct aatgacaata
     7501 actttccaac cctgtcggtt ccctccgctt ggagcaaagc gggagttgtc tagtacttta
     7561 taaataacct catctgttac aggatcgtct gtatattctc tcgcagagaa agttgtgtgc
     7621 attacttcat aaagttccat acctaatacc tcagttattt tttgaattat taattacatt
     7681 aaccagaaaa atgaatatct gaaaccttat ataaataatt gcatcgtatt tatataatgg
     7741 gatttcagga ggggtatatg agaaaagtat tagttttgat agcagtattt atggtagtag
     7801 gctgcacaca gtacgctcag aaagaagctg caaagcctat gactgatgtt gaaaaagcag
     7861 ggcagctctg ggccatgatg caaaaagaag attatagaaa cacatggaag atgtggccag
     7921 gaaaagaagc tttttataag ggagcacatc cgcatggtgc acttcttacc acttatgtga
     7981 gtgagtccgc atatgatgcc ctggttaaca aaaagggatc gatgccagat ggagcaatta
     8041 tcattaaaga aaactacatg cctgataaaa agcttggtgc tataacagtc atgaagaaaa
     8101 aagccggata taatcccgaa gccggtgatt atttttgggt taaattcggg ggtgacggct
     8161 cagttttaac caaggatatg gaagatggtt ctaagcttga tcttgcgggc aaggtgccgg
     8221 gatgtattgg atgtcatacg gctgtaaagg ctaacgactt tctttttaca gggcctatta
     8281 agtaatattg gttttcccta actaccatcc tatgtgcggc ggatacaaat gtgtgttcgc
     8341 cgttattaat ttagatagat taaattatat taatattata taatttatat atctatgagt
     8401 tatataattc tttacataat agcttcacta tttatctgtt ttttgttatc gatattactt
     8461 attaagagct cattcttgtt atggaatcat tataatactc gaaatgttag tgctgatctt
     8521 tccataaaaa atgtatttat gatttttatt cccaccactt ttgttttatg gattggtctt
     8581 actattttta tactagcaaa tctcaagatg cactgatctg aatttatgaa actcctcgat
     8641 agattaaaag ccaaatcagg gtttattaag gggctcgacc atgccgcgat tattgtaaag
     8701 gatatggaca ggtctattga gttttact
//
LOCUS       DBNGSV010000218         6172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1353600, whole genome shotgun sequence.
ACCESSION   DBNGSV010000218 DBNGSV010000000
VERSION     DBNGSV010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1353600"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC159U_07915"
     CDS             <1..190
                     /locus_tag="AC159U_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3337023.1"
                     /translation="RLGVPNSVGGLSDHTSNPLYATGVGLVLYGVKSGGEKMIKIKDE
                     NSMKKVLESMKNWFSEFF"
     gene            223..1332
                     /gene="ftsZ"
                     /locus_tag="AC159U_07920"
     CDS             223..1332
                     /gene="ftsZ"
                     /locus_tag="AC159U_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3337024.1"
                     /translation="MATFVIEETITDQGARIKVVGAGGAGGNAINTMIDRALGGVDFI
                     AANTDIQDLRKSKAATKIQIGTRTAKGLGSGGNPDMGREAAIEDQAKIEEALEGADMV
                     FITAGMGGGTGTGSSPIIAQTSRKMGILTVGVVTRPFGFEGPKRNKQAQSGINNLEDE
                     VDTLIVIPNDRLATVQKISIIEAFKRADEVLYQAVRGISDIITGTGYINVDFADVKSV
                     MGERGGKALMGTGYAEGPNRGIEAAEAAITSPLLEDISIDGAQGILLNVTAGPDFGIE
                     ELNEACSYVRERAHEDVNLIFGLIFDETLSEAVRMTVIATGINGEQSQEKQIENTGNG
                     IVTKLPTAVANNSEEAADEFDIPAFIRKRNIGERV"
     gene            complement(1608..2432)
                     /locus_tag="AC159U_07925"
     CDS             complement(1608..2432)
                     /locus_tag="AC159U_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HIR3337025.1"
                     /translation="MMKKYTVLNSLALLLLLTVNSFSDAVVNMEYKDNLSNTVNNNTN
                     SFKGSDMRMDFYEGGDEVTAAMIFKGDKNEMINLDHKAKKYFVMDKQTLLGLATQMNA
                     AMAELEKQMANMSPEEKAMMEKMMKGKMPSMNQEQKVEPVLKKAGTGEVSGYTCTKYE
                     VYKGSEKVRELCITNWSNIEGGTEIKNSILSMTNFMDELSKTMSESSGFMANTADFEK
                     NVFNEINKLNGFPVQTIDYTSGEVTGISTFKSSKKTNLEASVFNPPAGYKLEKFGM"
     gene            complement(2509..3177)
                     /gene="cofC"
                     /locus_tag="AC159U_07930"
     CDS             complement(2509..3177)
                     /gene="cofC"
                     /locus_tag="AC159U_07930"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR3337026.1"
                     /translation="MTFGIVPVKDLSKAKDRLSSVLPQKHRTTLAYAMLEDVLIALKN
                     SKLLDRKFIITLDKRAIEIASNLGIEIILETEQNGESASVDFAAAKCIEMGADSMLVI
                     PGDAPLITSSDIDLIIKEEKQLQAPYIIMVPAGDELGTNAILRNPPDIIPSRFGHDSY
                     NKHKEEANKKNIQYKTFNNPRIALDIDQPADLQLLIDQIGDSNTQRELINLGIMSHDL
                     NVRS"
     gene            3361..>6172
                     /locus_tag="AC159U_07935"
     CDS             3361..>6172
                     /locus_tag="AC159U_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR3337027.1"
                     /translation="MGNMKGDALNNELEAELKRELGAEIRFDEMSRILYSTDASNYEI
                     EPIGVVLPRTADDLAGAVQIAKRFNVAVLPRGGGTSLAGQAVGSALIVDTSKYLNKII
                     TVDAENNTVRTEPGIYLQQLNDNLKKFSLMFGPDPSTARIATVGGVVGNNATGAHSIL
                     YGMAGDNVESCRLVAADGDLVDLRVKSAGSFYPRLSVLKKKYSKLIHKDFPKHWRRAS
                     GYSLNYFMDKDFNPAKLLAGSEGTLGIASEFTLNLVKRPECTGLVVLQFAQIIEAMEA
                     VPVILEKEPSAVELIDKMLIELTRAHPGFTHLLTFIKGSPEAVLVVEFYGKTKKEVQI
                     KCRNLRTHLTSKKLKCDIRYALTDEQQSQVWQVRRAGLGLLMSRRDSYKPIPCIEDVS
                     VPVDKLSSYVKDIQNTISGFDTKAAFYGHASAGCLHIRPLVNLKTNNGVRVMQELTVQ
                     AMELAIKYGGVLSGEHGDGLQRSDLNEKLFGAKLYSVMKEIKSIFDPENMFNPGKIVD
                     APVCTEDLRYGEDYKTYDIKTHLEWKDDNGLAAAAEMCSGQGVCRKVDEGIMCPSYIA
                     TRDEKDSTRARANALRAVLSGRLPKQMLSSKEMHDVFDLCISCKACKTECPSAVDGAK
                     MKTEFLANYNKANGVSVRDKFFSYIHKLSDTASRVAPISNLITNNIITKLALPKIGIH
                     PKRALPEITRQTFTDWFYSRPKAKQNRANGKKVVYFHDTWVSFYTPEVGKSAVKLLEA
                     TGFDVVVVENRTCCGRPMISKGMVEEARNHALSNACALADFVRKGIPVIGTEPSCMLT
                     FRDEYPDILPGNEDMKLLADNSFLLEEFLYGRAKKELSNISWKKQGPSVLFHGHCHAR
                     ALIGNLASINILKEAGCSVIDSNAGCCGMAGSFGYESEHYEISKTIGEDRLFPTVNNC
                     STDTIIAVSGYSCSQQIEHFTAR"
BASE COUNT         1921 a         1086 c         1346 g         1819 t
ORIGIN      
        1 aagactcgga gtacccaaca gtgtcggggg tctatcggat catacatcca atccgctcta
       61 tgccaccgga gttgggctag ttttatacgg agtaaaatcg ggtggagaaa aaatgataaa
      121 aataaaagat gaaaattcta tgaaaaaagt actggaaagt atgaagaact ggttttcaga
      181 gttcttctaa aaaaaaacat atttttcaaa agggggaaaa ttatggcaac atttgttata
      241 gaggaaacaa tcaccgatca gggtgcaagg atcaaggtgg taggcgctgg tggtgcaggc
      301 ggaaacgcta taaacaccat gatagacagg gctcttggag gagtggattt tatagctgcg
      361 aatactgata tccaggacct gagaaaatcc aaggcagcga caaaaataca gattggcaca
      421 agaaccgcta aaggtttggg atcaggcggt aatcccgata tgggcaggga agctgctata
      481 gaagatcagg ccaagataga ggaagcgctc gaaggtgcgg acatggtctt tatcactgcc
      541 ggaatgggtg ggggtacagg aactggttca agccctatta ttgcccagac ttcgaggaaa
      601 atgggcatac tcacagttgg agttgtcaca aggccatttg gttttgaggg tccaaagaga
      661 aacaaacagg ctcagtcggg aattaataac cttgaagacg aagtggatac acttatcgtt
      721 atccctaatg acagacttgc aactgtacag aagataagca ttatagaagc ttttaaaaga
      781 gccgatgaag tattgtatca ggctgtaaga ggaatctcag atattattac cggtacaggt
      841 tatataaacg ttgactttgc tgatgtaaag agtgttatgg gagaaagagg tggcaaggct
      901 ctaatgggta caggttatgc agagggtcct aacagaggaa tcgaggctgc tgaggcagca
      961 atcacaagcc cgcttcttga agatatttca attgacggcg ctcagggaat attgctcaac
     1021 gttacagccg gcccggattt tggaattgaa gagctaaatg aggcctgttc ttatgtaaga
     1081 gagagggcgc atgaagatgt taatctcata tttggtctta tttttgatga aacactgagt
     1141 gaggctgtga ggatgactgt aattgcaacc ggaataaatg gtgaacaatc gcaggaaaag
     1201 cagattgaaa ataccggaaa cgggattgta acaaagcttc cgacagctgt tgcaaacaat
     1261 tcagaagaag ctgcagacga gtttgatatt cctgctttta ttagaaagag aaatataggt
     1321 gaaagagtgt aatagaatta tatagcagta aaagttttta acggcaatat gaatataatg
     1381 ccgatccata atttccccaa taataatagg ctgggtttga ttacaaaccc agctttttcc
     1441 ttcctgattt atttcaagat aatattagta agttgattaa cttgtcttgt tgaattaaat
     1501 agccgtgtat ttgcagccaa gaagattttt atccatctct tcattgcaat tgtttttatt
     1561 tccatcacac ttttcagata ctcatgggct tatactgtaa ttagaaatta catcccaaat
     1621 ttctcaagct tgtaacctgc cggagggtta aacactgatg cttcgagatt ggttttcttt
     1681 gatgatttaa atgttgaaat tcctgtgacc tcaccacttg tataatctat ggtttgtacc
     1741 ggaaaaccat ttagcttatt gatttcatta aacacatttt tttcaaagtc ggcagtgttt
     1801 gccataaatc ctgagctttc cgacattgtc tttgataatt catccatgaa attagtcatg
     1861 ctaaggatac tgttttttat ctctgttccg ccttcgatgt ttgaccagtt ggttatacaa
     1921 agttcccgta ctttttcgct tcctttatat acctcatatt tagtacatgt ataaccgctc
     1981 acctctccgg ttccggcttt tttcagtact ggttcaactt tttgctcttg gttcatagac
     2041 ggcatcttgc ccttcatcat tttctccatc atcgcttttt cttccggcga catatttgcc
     2101 atctgttttt caagctctgc catagcagca ttcatctgtg ttgctagtcc aagtagagtt
     2161 tgcttatcca ttacaaaata tttttttgct ttatgatcca ggtttatcat ttcgttttta
     2221 tcacccttaa agatcattgc agctgtgacc tcatcaccgc cttcataaaa atccattctc
     2281 atgtcactgc ccttaaatga attagtgtta ttgtttactg tattggaaag gttatcctta
     2341 tattccatat taactacagc atcagaaaaa gaattaacag ttaataaaag tagtaaggcc
     2401 aatgagttta aaacagtgta ttttttcatc atgatctccc ataaaatttt taaaaatata
     2461 ttttaactaa atctatttaa aaatcatata tatgcttgaa taattattct aacttctaac
     2521 atttagatca tggctcatta ttccaaggtt aattaattct ctttgagtat tactatcacc
     2581 tatttgatct attagcaact gcaggtctgc cggctgatca atatcaagag ctatccgcgg
     2641 gttgttaaat gttttgtatt gaatattttt cttgtttgct tcttctttgt gtttattgta
     2701 actgtcgtga ccaaatcgtg atgggataat atcgggtgga tttcttagaa ttgcattagt
     2761 accaagctca tctccggcag gtaccataat aatataagga gcttgtaact gtttttcttc
     2821 cttaataatc agatcaatat ctgaagaggt tataagaggg gcgtctccgg ggataaccag
     2881 catagaatct gctcccatct ctatacattt tgcagcggca aaatctactg atgcgctctc
     2941 accattttgt tcagtttcca ggattatttc tattcccaaa ttacttgcga tttctattgc
     3001 ccttttatcc agagtgataa tgaattttct atctaaaagt ttggaatttt taagtgcgat
     3061 aagcacgtct tcaagcatag cataggcaag tgttgtcctg tgtttttgag gtagaaccga
     3121 tgagagtcta tctttagctt ttgataagtc ttttacaggc acgatgccaa aagtcatttg
     3181 aaataatctg gtgttgagtc cttgttgtat ttgtacatat taaaaaaata gaatggtatg
     3241 ttacccaagt aatctgcaac ttaatatact aaatattaaa tttattttag ttatcagtat
     3301 ttactgtaag tgtagcaatg ataattcaat tcgacaataa atagtgagtt gatatagtac
     3361 atggggaata tgaaaggtga tgcgcttaat aatgaattag aggcagaatt gaagcgtgag
     3421 ctgggcgcag aaattcggtt tgatgaaatg agccgcattt tgtacagcac ggatgcaagc
     3481 aattatgaga ttgagcctat aggtgtagta cttccaagaa ctgcagatga cttggctggt
     3541 gctgtacaaa ttgccaaacg gttcaatgta gcagtgctac caagaggagg aggaacaagc
     3601 cttgcaggcc aggctgtagg cagtgcatta atagtagata cttcaaaata tctaaataaa
     3661 ataataacag tagatgctga gaacaataca gtccgcaccg agcctggcat ttatctccag
     3721 cagctaaacg ataaccttaa aaaattcagt ttaatgttcg gccccgatcc atccactgcc
     3781 aggatagcta cagtaggcgg ggttgtggga aataacgcaa caggggctca ctccatacta
     3841 tatggaatgg ccggagacaa tgttgagtcg tgcaggttgg tggcagcgga tggtgatctt
     3901 gttgatctgc gagtaaaatc cgcaggtagt ttctatccca gactatctgt actaaaaaag
     3961 aagtattcta aattaattca caaagacttt cccaaacatt ggaggcgggc ttcggggtac
     4021 agccttaact attttatgga taaagatttt aatcctgcaa agctccttgc tggtagtgaa
     4081 ggaactcttg ggattgcatc tgaatttaca ctaaatcttg tcaaaagacc tgaatgtaca
     4141 ggtcttgtag tattacagtt tgcacaaatt attgaagcca tggaagctgt gcctgttatc
     4201 ctggaaaaag agccgtcggc agtagagctt atagacaaga tgctcataga acttaccaga
     4261 gcgcacccgg gatttaccca tttgctcact tttataaaag gcagtccgga agccgtactg
     4321 gtagtcgagt tttatggaaa gaccaaaaaa gaagtgcaaa ttaaatgcag gaatctcaga
     4381 actcatctca caagtaaaaa gttaaaatgt gatatcaggt atgcgctaac agatgaacaa
     4441 caaagtcagg tatggcaggt ccgcagggcg gggttaggtc ttctaatgag caggcgtgat
     4501 agttataaac ccataccatg cattgaagat gtttcggttc ctgtagataa gctttccagc
     4561 tatgttaagg atattcaaaa tactataagt ggatttgata caaaggctgc cttttacgga
     4621 catgcaagcg cagggtgtct gcatataaga ccgcttgtta atctgaaaac aaataatggt
     4681 gtaagagtca tgcaggaact aaccgtacag gccatggaat tagcaataaa atatggtgga
     4741 gtgctaagcg gtgagcatgg tgatggactt cagaggagcg atcttaatga aaagctgttc
     4801 ggtgccaaac tgtatagtgt gatgaaagaa ataaagagca tttttgatcc ggaaaacatg
     4861 tttaatcccg gaaaaatagt tgatgccccg gtttgtactg aagatctgcg ttatggtgaa
     4921 gattataaaa cctatgatat aaagactcat ttggaatgga aagatgataa cggtttagct
     4981 gcagctgccg agatgtgcag tggtcagggt gtttgcagaa aggttgatga aggaataatg
     5041 tgtccgtcat atattgctac gcgggatgaa aaagattcta cgcgcgcaag agcaaatgcc
     5101 cttagggctg ttctatctgg aagacttcca aaacaaatgc tttcaagtaa agagatgcat
     5161 gatgtatttg atctttgcat atcctgtaaa gcctgcaaaa cagagtgtcc gtcagcagtt
     5221 gacggggcaa agatgaagac agagttttta gcaaactaca ataaagccaa tggcgtatcc
     5281 gtaagagata aattcttctc atacatacat aaacttagtg atactgcatc cagagttgca
     5341 cccatttcaa atcttattac aaataatata attactaagc ttgctttacc caaaattgga
     5401 atacatccca aaagggcact tccagaaata accaggcaaa catttacaga ttggttttac
     5461 agcagaccga aggcaaaaca gaacagagct aacgggaaaa aagtagttta tttccatgac
     5521 acatgggttt cattttatac tcctgaagta ggaaaatccg cagttaaatt attagaagcc
     5581 acaggttttg atgtggttgt tgtagaaaac agaacatgct gcggaagacc catgataagc
     5641 aaggggatgg ttgaagaagc aagaaatcat gcactcagta atgcttgcgc tcttgctgat
     5701 tttgtaagaa agggaattcc tgtaattgga acagagccca gctgtatgct taccttcagg
     5761 gatgaatatc cggatatttt accaggtaat gaagatatga aacttcttgc tgataactca
     5821 tttctgcttg aagagttctt gtatggtcgg gccaaaaaag aactgtctaa tatctcatgg
     5881 aagaagcagg ggccttcagt actatttcac gggcactgtc atgcacgagc gctaatagga
     5941 aatttggcat caataaatat tttaaaagag gcgggatgca gcgtaataga tagcaatgca
     6001 ggttgctgcg gtatggctgg aagctttggt tatgaatcag agcattatga aatatccaaa
     6061 acaattggtg aagaccggct ttttcctacg gttaataatt gcagcactga taccataata
     6121 gcagtatcag gatactcgtg cagtcagcag attgaacact ttacagctag aa
//
LOCUS       DBNGSV010000219         4290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1839125, whole genome shotgun sequence.
ACCESSION   DBNGSV010000219 DBNGSV010000000
VERSION     DBNGSV010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1839125"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC159U_07940"
     CDS             <1..212
                     /locus_tag="AC159U_07940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3337028.1"
                     /translation="DYLIYKQIHPNVLTVSSLIVSMISGLFFYFGLIFLAGLLMLSGS
                     TFDIFDGRVARAQNLSSEAGAFFDS"
     gene            288..572
                     /locus_tag="AC159U_07945"
     CDS             288..572
                     /locus_tag="AC159U_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337029.1"
                     /translation="MLLIVSSTMMVSYAKARAEGLGAKCDVGIMQRTERVVYLGLFSV
                     FNFVGNLVTDALKLEGRDYLLKFALIVMLIFSVYTVIQRMAATMKELNER"
     gene            690..1208
                     /gene="msrA"
                     /locus_tag="AC159U_07950"
     CDS             690..1208
                     /gene="msrA"
                     /locus_tag="AC159U_07950"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3337030.1"
                     /translation="MSNTTERAVLAGGCFWGMQDLIRKLPGVISTRVGYSGGTVQYAT
                     YRNHEGHAEAIEIFFNPDVISFRTLLEFFFQIHDPSTPNQQGNDRGSSYRSAIFYTSN
                     EQRQTALDTIADVDASGIWPGKVVTEVAPAGDFWEAEPEHQDYLQRYPSGYTCHYPRE
                     NWVLPKRKAETG"
     gene            1418..1630
                     /locus_tag="AC159U_07955"
     CDS             1418..1630
                     /locus_tag="AC159U_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR3337031.1"
                     /translation="MKQNMGTADKGVRILVAIVIFALYFTGTIGGTLGIILMVLAVVF
                     VGTSLLGSCPLYIPLGLSTKKDSKSE"
     gene            1906..2244
                     /locus_tag="AC159U_07960"
     CDS             1906..2244
                     /locus_tag="AC159U_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337032.1"
                     /translation="MGKKDRKFHGILFIAVSLLFFGIYSLSALETQNIEGYLICIAPD
                     DEGNIKIEAEFSQCAGSIALVNLENEIYTISGAQNYIDKLNDAPKRRMGVLMDQKLRA
                     RCMVTKGRYI"
     gene            2241..2540
                     /locus_tag="AC159U_07965"
     CDS             2241..2540
                     /locus_tag="AC159U_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337033.1"
                     /translation="MMAGAAKYIDEGKTEKVKGTIYCLFPDYKKSYMKYKLTNKPCYS
                     ARPHAHFLHTDGNEIIAITGSEEHIKHIESATERKDVYLTGSLSGSKNSRYINLK"
     gene            2774..4105
                     /gene="ffh"
                     /locus_tag="AC159U_07970"
     CDS             2774..4105
                     /gene="ffh"
                     /locus_tag="AC159U_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3337034.1"
                     /translation="MFEGISDKLSNSLKEIRGTSRISEDNIKETLRQVRLNLLEADVN
                     FQVVKGFIDSVRERSLGTVVGEKLNPGQQFVKIVNEELVKVLGEATTEVDLHASPPVV
                     FMLVGLQGSGKTTSIAKLAKYLRDTHKRNPYLVPADIYRPAAIDQLKVLADSEGFGVF
                     DSSADMKPLDICNQARDAALTGGYDVMLIDTAGRLHIDEELMNELVELKNGLNPHNIY
                     FVADAMTGQDAVNVASSFNDTLDISGVILTKMDGDARGGAAMSIKAVTGKPIMFFGTG
                     EKLDALEVFHPDRIASRILGMGDVLTFIEKAQTAIEEEKAEKMAKKILKNRFSLGDFK
                     ESMLAMRKMGSLEEMAGMVPGIKDLTKNPKAMGMAEKEIDKTIAIINSMTKKERRDHT
                     LINGSRRKRIAAGSGTSVPDVNRMMKNYAKMRKMMKNMGNMGKLAQRLMKF"
     gene            4140..>4290
                     /gene="rpsP"
                     /locus_tag="AC159U_07975"
     CDS             4140..>4290
                     /gene="rpsP"
                     /locus_tag="AC159U_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3337035.1"
                     /translation="MVKIRLMRMGSKKRPFYRIVAADSRAPRDGKFLEILGHYDPRTE
                     PNKIEI"
BASE COUNT         1372 a          820 c          976 g         1122 t
ORIGIN      
        1 aagattatct gatttataag caaatacatc caaacgtcct tacggtatct tctttgatag
       61 tatccatgat ctcggggctc tttttttatt tcggtctgat atttttggca ggtcttttaa
      121 tgctctcagg ctctacattt gatatttttg acggcagggt agcacgtgca caaaacctaa
      181 gcagtgaggc gggagctttt tttgattcat gactggacag aattgctgaa gcctttatat
      241 atcttggtct tttaggctac tttcagggta cagtatttat atacgtagtg cttctaatcg
      301 taagctctac aatgatggta agctacgcta aggctcgtgc cgaagggctt ggagcaaagt
      361 gtgatgtggg aataatgcaa agaaccgaaa gagtggttta tctggggcta ttctcggtct
      421 ttaacttcgt tggaaatctt gttacagacg ctctgaaact agaaggcaga gactaccttc
      481 taaaatttgc cctaatagtc atgctcatct tctcagtcta caccgtaatc caaagaatgg
      541 cagctacaat gaaagaactt aatgaaagat agttctttct ggttaaatgc tataaatctg
      601 catctattaa tctaaacccc acctctaacc ttgaataaaa ggttataatt aatatattaa
      661 aatttatcca aagcaggagg gataaaaata tgagcaatac tacagaacgc gcagtgcttg
      721 caggtggttg cttttgggga atgcaggatc ttattagaaa actgcccgga gtaatttcta
      781 ctcgtgtggg ctacagcggc ggcactgttc aatacgctac atacagaaac cacgaaggac
      841 atgctgaagc cattgaaata ttctttaacc ccgatgtgat aagtttcaga acactcctgg
      901 aattcttctt ccagattcat gatccaagca cacctaacca acagggaaat gacagaggaa
      961 gctcataccg ctctgcgata ttctatacat ctaatgaaca gagacagact gcgcttgata
     1021 cgattgcaga tgtggacgca tctggaatat ggcccggcaa agtagtaacg gaggttgcac
     1081 ccgcgggaga cttctgggag gctgagcctg agcatcagga ctatctgcaa cgctacccaa
     1141 gcggctacac atgccactac ccaagagaaa actgggtgct tcctaaaaga aaggcagaga
     1201 ctgggtaacc ttctgaaact taataaaacc tcacgcatcc aacaaattta tcctgtataa
     1261 ttatataaga gactaaatgt ctgaaaccgg cacaaaaata gctaaactaa ttatcgtctg
     1321 cgtgctgtgc gcagccttag tcacgggtat cgtttatctt gtaaaatggc ttttttaggc
     1381 aggtttaatt ccagtatata aaaggaggtt taacgagatg aaacaaaata tgggaacagc
     1441 tgataagggt gttaggattt tggtggccat agtcatattt gcgctgtatt tcacgggcac
     1501 tatcggcggc acgctgggaa taattctaat ggttcttgcc gtcgttttcg taggcacaag
     1561 ccttctgggt tcatgcccgc tctacattcc tctaggtctt tctacgaaaa aagattcaaa
     1621 gtcagagtag cgtacaggct ctttccagaa atctgattta gcaaataaac ctcgaacggg
     1681 ggagttttgc cctttataga agcattttat taacttatac aaagtaacca gtaagccttc
     1741 acccaaaagc ttcgggtcaa aaggtcaatg gatttgggtc tagtgtacat aggctgaccg
     1801 taagacccta aatggtttga atacctttat tccatttcta aacataatga tttaaaataa
     1861 taggtataaa tattttcaaa ataactatcc agggggaaag aaactatggg aaagaaagat
     1921 agaaagtttc atgggatatt atttattgca gtctctctcc ttttttttgg aatttattct
     1981 ctaagcgctc tagagacgca gaatatagaa ggctatctaa tctgcatagc acccgacgat
     2041 gaaggaaaca taaaaataga ggcagaattc agccaatgtg ccggcagtat agcgcttgtt
     2101 aatctggaaa atgagatata cacgataagc ggcgcgcaaa actatataga caaactaaac
     2161 gacgccccca agagacgaat gggagtgctg atggatcaaa agttacgggc acgctgcatg
     2221 gtcacaaaag ggcgttacat atgatggcag gagctgcaaa gtatatcgat gaaggcaaga
     2281 ccgaaaaggt aaaagggacg atctactgcc tgtttccaga ttataaaaaa tcatatatga
     2341 aatataagct tacaaacaaa ccctgctaca gtgccaggcc tcacgcccac tttttacata
     2401 cagatggtaa tgagattatt gccattacag gaagcgaaga gcatataaaa cacattgaaa
     2461 gcgcaaccga aagaaaggat gtttatctaa ctggtagttt atccggaagt aaaaacagca
     2521 gatatatcaa tctgaaatag tatctcgctg ttttctaaat attatcactt caggtcttcg
     2581 ggtcaaaggg ttagggtcta tggtggaacg ggtgaccgta agaccgtaac tggttttctt
     2641 gctcttattc catttctaaa cataatgatt taaactaata ggataactgc cgataattag
     2701 ttgggatcat tgacaattta tatgagttat gcacaatttg actctttaaa attgatgctt
     2761 atatagaata agaatgtttg aaggaatatc cgataaactt agcaattcgt taaaagagat
     2821 aaggggtaca tcccgaatct ccgaagacaa cataaaagag accctgcgtc aggtaaggct
     2881 aaacctcctg gaggccgatg taaactttca ggtggtaaag ggttttatag actctgtgag
     2941 ggaaaggtcg cttggcaccg tagtgggtga aaagctaaac ccgggccagc agtttgtaaa
     3001 gattgtaaat gaagagctgg ttaaagtact cggtgaagcg actacagagg ttgacctgca
     3061 tgcctccccg cccgtagtgt ttatgctcgt ggggcttcag ggttctggta aaacgacatc
     3121 aattgcaaag cttgcaaaat acttaagaga tactcataaa agaaatccat acctcgtacc
     3181 ggctgatatt tacagacccg ctgctatcga tcagctaaag gtacttgcag actctgaagg
     3241 tttcggcgtg tttgacagct cggccgatat gaagccgctt gacatctgca atcaggcccg
     3301 tgatgccgca cttacaggcg gatatgacgt gatgcttatt gacactgcgg gtaggctgca
     3361 tattgatgaa gagctaatga atgagcttgt tgagcttaaa aacgggctta atcctcacaa
     3421 tatatacttt gtagcagacg ccatgacggg gcaggacgcc gtaaacgtag catcaagctt
     3481 caatgatacc ctggacataa gcggcgtaat actcacaaag atggacggtg atgcccgcgg
     3541 gggtgcggca atgtccatta aggctgttac cggcaagccc ataatgtttt ttggaacggg
     3601 tgagaaatta gacgcccttg aagtctttca tccagacagg atcgcttcta gaattctggg
     3661 catgggtgac gtacttactt tcatagagaa agcacagact gccatcgaag aagaaaaagc
     3721 tgaaaagatg gcaaagaaaa tcctcaagaa caggttttct ctcggggact tcaaagaatc
     3781 catgcttgct atgagaaaga tgggctcact cgaagaaatg gccgggatgg tgcctggcat
     3841 aaaagacctt accaaaaatc caaaggctat gggaatggcc gaaaaagaga ttgataagac
     3901 aattgccatt atcaattcca tgaccaaaaa agagagaagg gaccatactc ttatcaatgg
     3961 aagcagaagg aaaaggattg ccgcaggaag cggaaccagt gtgcccgatg ttaacagaat
     4021 gatgaaaaat tatgctaaaa tgagaaaaat gatgaaaaac atgggaaata tgggtaaact
     4081 agcacagagg ttaatgaagt tttaaaataa agcgaaaagc actaaggggg ttttttgaat
     4141 tggttaaaat aagacttatg agaatgggtt caaagaagag gccgttttac cgtattgtag
     4201 cggctgattc cagagcacct agagacggta aatttcttga gatccttgga cattacgatc
     4261 caagaaccga gcccaacaaa atagagatta
//
LOCUS       DBNGSV010000220         8582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1285720, whole genome shotgun sequence.
ACCESSION   DBNGSV010000220 DBNGSV010000000
VERSION     DBNGSV010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1285720"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="AC159U_07980"
     CDS             complement(<1..543)
                     /locus_tag="AC159U_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3337036.1"
                     /translation="MEDIKILVVEDNELNSQLICESLTTMGYENNHRVKNYDSAIEFV
                     ENNKTDLILMDIMLKGSKDGVTIAKKIQTEHDIPIIYITGYAKDKIIKRVKDTNPYAF
                     ITKPFKFEELKANVEIALTRHKSNQKKNIERLKYEQAFVNMHCGVITTDDSGVVLFIN
                     KVAEILTGFKSDEATKLRFDQ"
     gene            1094..1762
                     /locus_tag="AC159U_07985"
     CDS             1094..1762
                     /locus_tag="AC159U_07985"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337037.1"
                     /translation="MSNNIKFEIKDLSLSRNSSVIFNKINLTIANGSISIITGKSGSG
                     KTSLLRLLNRLEDPVSGSIYYKEKDLKEYDVLQLRKDISLVQQIPIIIGNSVKDNLLL
                     QSTLTKQPYPSDETLITTLDMCGLSENFINKNAQLLSLGEKQRVCIARSIINKPDVLL
                     LDEPTSSLDFENSLSILHLINDLNKELGITIILVTHKIDEARYLNADYYTLSNGIINK
                     GINY"
     gene            1766..2557
                     /locus_tag="AC159U_07990"
     CDS             1766..2557
                     /locus_tag="AC159U_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3337038.1"
                     /translation="MEQNTSSLIYLSYLDLILGFSLILIAAGLSKWKELKLEKDLIIA
                     SLRTFLQLIGVGFILKYVFESNNSILVLFFIFVMITVAVFTVIRDKESIFSNLSFIVS
                     FALISSSGITVFLVTQIIINIDPWYSPQYLIPITGMVIGNSINGASIAIDRLDSEIRE
                     KKDKIEALLCLGATSHQSCADIIRSSMMASLIPTINSMMIVGIVTFPGIMTGQILSGI
                     SPISAIKYQIIIMYMIAFSVTFCSYLIINLRYKSYFTKDHQYIHK"
     gene            2653..2910
                     /locus_tag="AC159U_07995"
     CDS             2653..2910
                     /locus_tag="AC159U_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3337039.1"
                     /translation="MGCCFKAIGDERRRKILTILSKGSASAGDISSKFKISQPTISNH
                     LKILREAGLIKEKKIAQSRIYSLNQDRIKKVIATLSEIVEP"
     gene            2923..3381
                     /locus_tag="AC159U_08000"
     CDS             2923..3381
                     /locus_tag="AC159U_08000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3337040.1"
                     /translation="MSKDKTDLKLASSKHIPALLDFIQSYYDFESITYLENKVEKALR
                     SLLNSEDIGRVWLITFNDEPVGYIILCFSYSLVSYGQDGMIDEFYIKEKYRNKGIGTE
                     VIKQVIKNSKKLGLKSLYLEVNKHNKEAQSFYNSLKFKKRDNYFLMNLDL"
     gene            complement(3385..4281)
                     /locus_tag="AC159U_08005"
     CDS             complement(3385..4281)
                     /locus_tag="AC159U_08005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3337041.1"
                     /translation="MDLKILDINFFNPNVIACHLIKTSEGPILIETGPDSVFMNLEKA
                     LNDNGYQVSDIKHVFVTHIHLDHSGAAWHFAKEGAKIYVHGRGAPHIIAPEKLMKSAA
                     MIYGDDMDRLWGTVENVDENLVYAIEDQEVITIGDTKIKAIETPGHASHHHAYQIEDI
                     LITGDVTGNRINLGPILPPTPPPDINVELWQESIQKIRDINPSTIYPTHFGGQNGITE
                     INAHLEELENTLLDWTEWIGVRVKDGKTDEEIIPEFEEYASNILKSANVSEIDYRSYE
                     LSDPFFMNVGGLTRYWRKFRLS"
     gene            4358..5533
                     /locus_tag="AC159U_08010"
     CDS             4358..5533
                     /locus_tag="AC159U_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3337042.1"
                     /translation="MYNIRKILEIDMINHDRLTEYTMDLIKIDSPSKHEKDVAMKLKH
                     DMEHIGADCSFDDADKKVNGNVGNLTVKLKGNTDAPPFFFSSHMDTVSPGIGIKPHVK
                     DGIMRSDGTTILGSDDKSGVAIIVEIIKSLKENNIPHGDIEVAFTICEEIGLLGAKYY
                     DVNQLSAKHGIVLDSSTPDRLVLRCPSAEDLHFTVHGVEAHAGLCPEDGISAIKVASD
                     AISTMTFGRIDHETTSNIGIINGGIATNIVTGKVDVVGEVRSHDEQKLQAQIYHMQEC
                     FTNAAKKYKINLDGKELNAKVEFSNERSYDRMDAGDDSYITKLVLKAVDNLGHKVKLH
                     TTGGGCDANYFNKYGISCANLGTGMYELHTVNEYLVLEEQQRCAKIMLETVRLNAEN"
     gene            5533..5904
                     /locus_tag="AC159U_08015"
     CDS             5533..5904
                     /locus_tag="AC159U_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HIR3337043.1"
                     /translation="MKALILIDHGSKVEQANNLLVQIVGLLNKKQNTGFDFITYSHME
                     LCNPTIEDAFYLAASKGATEIIVHPYFLAPGRHSKTDIPNMVKEAASKYTNIKYKITE
                     PLGIHDKILEVILERANHEKV"
     gene            5891..6637
                     /locus_tag="AC159U_08020"
     CDS             5891..6637
                     /locus_tag="AC159U_08020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3337044.1"
                     /translation="MKKYKYIFFDLFDTLIDFNYTMLPQISIDGEKHNTTSRLVYDVF
                     SKFYPDISFDTFYKPFMETFHEFQQLKKIDNREYPNQQRFNMLIDKLNLNHIDNKDEV
                     LSSMTEVHMSGLASATTFPENNKTTLEYLKKLDYRFALISNFDYAPTAYALLDQYDLK
                     KYFDRIYISIEVGWRKPHKNIFQIALDECEVNNDEVIHVGDNFGADIVGAFNQGIDSI
                     WINRKDEELTHDSVTPNIIVSKLYELTSLF"
     gene            complement(6634..7806)
                     /locus_tag="AC159U_08025"
     CDS             complement(6634..7806)
                     /locus_tag="AC159U_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337045.1"
                     /translation="MKYKKMKILFTVLFVSLFFSSCTKKVQDNSAELPTDAQNEMYSQ
                     EQLDFKKWVNIDSALQQEFKNQYNDTRTKRPLYEKYYDIIGPVAMLEFLEEKYTLCHS
                     QAHDVGKVTYSRIKDINKSLAVCGNRCTNACMHGVVTEAFGSKSIEEIISLSKTFCES
                     EQMNKLHKKGNCMHGLGHAFMIVSGHDIRKSIDACDTFTDKSFSYYCATGVFMEYRDN
                     LRNKRQKLPVSTNQKNEHSHHHHHSHESEMNMNEDITDFYPCDKYNRYPAACYRYMFP
                     LIRSKLKIDRTELINKCMNLPDIERRGCFHGLGLMYIVSVSRKPSNITKLCNQGDYED
                     KVMCVEGVIEKLSDMKESSALEACNHLDGKLSEVCMGAATEKMYRLNKPTMNLYVN"
     gene            complement(7812..>8582)
                     /locus_tag="AC159U_08030"
     CDS             complement(7812..>8582)
                     /locus_tag="AC159U_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337046.1"
                     /translation="INMNNKYIYGVQLIALLILLNFISFMMGGTDLRIFLFSREGFYM
                     LSFCVFFILFVYYLIRFYKGKSNSNNLRFIPSNKALVILPIILFTVGLFPYLGVKNIQ
                     VFTMFSNLNTENSKSNHFFIPASIQIFDNLSNVVHIKSSSLGGLDKFSKPVVTRPIRQ
                     TQILLPKNYIRYQETRDRSVKEKYYFAITYKHLANIITEVSERGVKNIEIEFERNGKL
                     FNIKNAENDPELSTASLIQRKFLGFRAVPVNNPGVCMW"
BASE COUNT         3001 a         1434 c         1320 g         2827 t
ORIGIN      
        1 ttgatcaaac ctgagttttg tagcctcgtc ggatttaaat ccagtaagaa tttcagccac
       61 tttattaata aataatacta ctccgctatc atctgtagta atcaccccac agtgcatatt
      121 aacaaatgcc tgttcgtatt ttaaacgctc tatgttcttt ttttgattgg acttatgcct
      181 tgtaagagca atttctacat tagccttcag ttcttcaaac ttaaaaggtt ttgtgataaa
      241 tgcataggga tttgtatcct taactctttt gattatcttg tcttttgcat atcccgtaat
      301 ataaataata ggtatatcgt gttctgtttg tattttttta gcaattgtta ccccatcttt
      361 tgaaccctta agcattatgt ccattaagat caagtctgtt ttattatttt ctacaaattc
      421 aatagctgaa tcatagtttt ttactctatg gttgttttca taacccattg tagtaagaga
      481 ttcgcaaatt aattgtgaat taagttcatt gtcttcgaca actagaattt ttatgtcttc
      541 catagctgtt tatataaaaa aaagagttaa tctgcagtaa aaacctgacc ctatcacata
      601 cattatgtca taattcttat tttaatgtca acatattttg tattactgta taattactta
      661 acagatattt atttttaata aattagtaat taagtctcat tattaatgat aatttactaa
      721 acaggcatta atttatagaa atatcatgta attcagcatt actgttatct aataaatcat
      781 gcatatagat attgatacaa aatagaagta aaaggaaaaa tattagttaa agtattactt
      841 taagtaatac aataattcaa cttcaagcat acactaggaa aatcatttta cacattaagt
      901 atattactca ttatttacag ttatttacag agcatatata atataatact ttaattatgt
      961 gttataaaat ctacctaagt ttattattaa acaaatagtt aaagtaataa tatatataat
     1021 atatacagta tgttatacgt tatacttaat atttaacttt atgtacgcta ttctaatgat
     1081 atttaaataa ataatgtcca ataatataaa atttgagatt aaggatttaa gtctaagtag
     1141 aaatagttct gtaatattta ataaaattaa tttaactatt gcaaatggtt caatttcaat
     1201 aattacaggt aaatcgggta gtggtaagac ctcgctatta cgtcttttaa acagactcga
     1261 agaccctgtc tccggttcta tttactataa agaaaaggat ctaaaagaat atgatgtatt
     1321 acaacttaga aaagatatat cccttgtaca gcaaatacct attattatag gtaatagcgt
     1381 taaagacaat cttctattac agtcaacttt aacaaagcag ccctaccctt cagatgaaac
     1441 cttaataacc actttagaca tgtgtggatt atcagagaat tttattaata aaaatgctca
     1501 actattgtca ttgggtgaaa aacaaagagt atgtatagcc agatcaataa taaataagcc
     1561 tgatgttttg ttgctagatg aacctacttc atcactggat tttgaaaatt ctctatctat
     1621 tttacatctc ataaatgatc ttaacaaaga actaggtata accataatat tagttactca
     1681 taaaattgat gaagccagat atctaaatgc agattactac acattatcaa atggaataat
     1741 taataaggga ataaactatt aataaatgga acaaaatact tcttctttaa tatatctatc
     1801 atatttagac ttaattttag gatttagtct gatactcata gctgcaggat tatcaaagtg
     1861 gaaagagtta aaactagaaa aagatttaat aattgccagt ttaagaactt ttctacaact
     1921 aataggcgta ggatttatat taaaatatgt atttgaatca aataacagca tattagttct
     1981 gttttttatt tttgtaatga taactgttgc cgtatttact gtaataagag acaaggaatc
     2041 tatattttcc aatttatctt ttatcgttag tttcgctctc atctcgagct ctggtattac
     2101 tgtgtttcta gttacacaaa ttatcattaa cattgaccca tggtatagcc ctcagtactt
     2161 aataccaata acaggaatgg taattggcaa cagtattaat ggtgcttcaa ttgcaatcga
     2221 cagactagat tcagaaataa gagagaagaa agataagatt gaagcacttt tgtgcctagg
     2281 agctacatca catcagtctt gtgccgatat aataagatct tcaatgatgg catctctcat
     2341 tccaactata aattcaatga tgatagttgg aattgttaca tttcctggaa ttatgacggg
     2401 tcaaatactt tctggtattt caccgatatc agcaataaaa tatcaaataa taataatgta
     2461 tatgattgca ttttctgtaa cgttttgttc atatttaata attaacttaa gatataagag
     2521 ttatttcaca aaggatcatc aatatattca caaataattt cattattatc aaaatatcta
     2581 tttaatttct tagttattaa attataataa aagcgttata acaattttac taacaaaagg
     2641 agaataaaat taatgggttg ttgttttaaa gctataggag atgagcgcag aagaaaaata
     2701 cttactatac tcagtaaagg aagtgcttca gccggagata tatcatctaa atttaagata
     2761 tcacaaccaa ctatctcaaa ccatttaaaa atattaagag aagccggcct cattaaagaa
     2821 aaaaaaatcg cccaaagcag aatttattcc ttgaatcaag atagaataaa aaaagttata
     2881 gcaacactga gtgaaattgt agaaccataa caaaatgaaa cagtgagtaa agataaaaca
     2941 gacctaaaat tagcttcttc aaagcatata ccagcgcttt tggattttat acagtcttat
     3001 tatgattttg aatccattac ataccttgaa aacaaagtag aaaaggcact taggagtctc
     3061 ttaaactctg aagacattgg aagagtctgg ctcataacat ttaacgatga acctgtgggt
     3121 tatattatac tgtgttttag ttactcactc gtatcatatg gacaagacgg catgattgat
     3181 gagttttata taaaagaaaa atatagaaat aaaggaattg gtacagaagt aataaaacag
     3241 gttataaaaa actctaaaaa actcggcctt aaaagtcttt atttagaagt aaataaacac
     3301 aacaaagagg cacagagttt ttataacagc ctcaaattca aaaaaagaga caactatttt
     3361 ctaatgaatt tagatttata actattaact gagcctgaat tttctccaat atcttgtaag
     3421 ccccccaaca ttcataaaaa aaggatcaga aagctcataa gatctgtaat ctatctcgct
     3481 gacattagct gattttagta tattactggc atactcctca aactccggaa ttatttcttc
     3541 atctgtcttg ccgtctttaa ctctgactcc aatccattca gtccagtcta aaagcgtgtt
     3601 ttctagctct tcaagatgag catttatttc tgttattcca ttctgtccgc caaagtgggt
     3661 aggatatata gtagaaggat taatatccct aattttttgg atcgattcct gccagagctc
     3721 tacattaata tcaggaggag gtgtgggagg aagtataggt ccaagattaa tcctgtttcc
     3781 cgttacatct cctgttatca gaatatcttc tatctgatat gcatgatggt gagaagcatg
     3841 gcccggtgtt tctatagcct ttatctttgt atccccaata gttattactt cctgatcttc
     3901 aatagcataa accaggtttt catccacatt ttccacagta ccccaaagcc tgtccatatc
     3961 atcaccgtag atcatagcag cagatttcat caacttttca ggagcaatta tatgaggcgc
     4021 ccctcttcca tgcacataaa tctttgcacc ttcctttgca aaatgccatg cagctcctga
     4081 gtggtctaaa tgtatatgag taacaaatac atgtttaata tctgatactt ggtatccgtt
     4141 atcatttagg gctttttcaa gattcataaa cacagaatct ggaccagttt ctataagaat
     4201 cggtccctca cttgttttta taagatgaca tgctattaca ttcgggttaa aaaagttaat
     4261 atccagtatt tttaagtcca tgattattcc tctatagtaa atttatctaa aaataaccga
     4321 gtaaatgtca taaaacaaat tacagtatat attatttatg tataatataa gaaaaatatt
     4381 ggagattgat atgataaacc atgacaggct aaccgaatat actatggatt taataaaaat
     4441 agacagtcct tcgaagcacg aaaaggatgt agccatgaaa ttaaagcatg acatggagca
     4501 tataggtgct gattgttctt ttgatgatgc tgataagaag gtaaacggga atgtaggaaa
     4561 tttaactgta aaacttaaag gaaataccga tgccccccca ttcttctttt catcacatat
     4621 ggatacggta agtcccggga tcggaattaa acctcatgta aaagacggaa taatgagaag
     4681 cgatggtaca accatacttg gaagtgatga taaaagtggc gttgcaataa ttgtagaaat
     4741 aataaaatct cttaaagaaa acaatatccc tcacggagat atagaagtag catttacaat
     4801 atgcgaagag atcggattgt taggcgctaa atattatgat gtaaaccagc tttcagccaa
     4861 acacggcatt gtacttgata gcagcacccc cgatagactg gtactaagat gcccgtctgc
     4921 agaagacctt cattttacag ttcatggagt tgaggcccat gcaggtcttt gtccagaaga
     4981 cggcattagt gctataaagg tagcatctga tgctatatcc accatgacat ttggacgaat
     5041 agatcatgag acgacttcaa atattgggat tataaacggc ggtattgcaa caaacatcgt
     5101 taccggcaaa gtagatgttg tgggagaggt aaggagccat gatgaacaga aattacaagc
     5161 tcagatatat catatgcagg aatgttttac taatgctgcc aagaaataca agattaattt
     5221 agacggaaag gaactaaatg caaaagttga atttagcaat gaaagatctt atgacagaat
     5281 ggacgcaggc gatgattcat atattacaaa acttgtttta aaggccgtag ataatcttgg
     5341 tcataaagta aagcttcaca ccacaggtgg cggctgtgat gctaattatt ttaacaaata
     5401 tggtataagc tgcgcaaatc ttggaacggg gatgtatgag ctccatacag taaatgagta
     5461 tcttgttctc gaagagcagc agcgctgtgc aaagataatg ctcgagaccg ttagactcaa
     5521 tgccgagaac taatgaaagc actcatactc atagatcatg gaagcaaagt agaacaggca
     5581 aataatcttt tagtgcaaat agtaggtctg cttaataaga aacaaaacac tggttttgat
     5641 tttataacat actcccatat ggagctttgt aaccctacaa tagaagacgc attctatctt
     5701 gcagctagta aaggagctac ggaaataatt gtccatccat acttccttgc accaggaagg
     5761 cactctaaaa ctgatatccc caatatggtt aaagaagctg cttctaagta tactaatatt
     5821 aaatataaga ttaccgaacc acttggaatt catgacaaga tactggaggt tatactagaa
     5881 cgagcaaatc atgaaaaagt ataaatatat attctttgac ctttttgata ctttaattga
     5941 cttcaactat acaatgttgc cacaaataag cattgacgga gaaaaacata atactacaag
     6001 cagactagtt tatgatgtat ttagtaagtt ctaccccgac atttcttttg atactttcta
     6061 taagcccttt atggaaactt ttcatgaatt tcaacaatta aaaaaaatag acaacaggga
     6121 atatccaaac caacagagat tcaatatgct tatcgacaaa ttaaatctca atcatataga
     6181 taataaggat gaagtgcttt ccagcatgac agaggttcat atgtccggac ttgcgtctgc
     6241 tactactttt ccagaaaata ataagacgac actcgaatat ctaaagaaac tggattacag
     6301 gtttgctcta atctctaatt ttgactatgc ccctactgct tatgccctat tggatcagta
     6361 tgatctcaaa aaatattttg ataggattta tatttcaatt gaagttggct ggagaaaacc
     6421 gcataaaaac atttttcaga ttgcacttga tgagtgtgag gtaaataatg acgaagtaat
     6481 ccatgttggt gacaatttcg gtgcagacat agttggagcc tttaatcagg gaatagacag
     6541 tatatggatc aacagaaagg atgaggaact aacacatgat tctgtaacac ccaacattat
     6601 agtatcaaaa ttgtatgagc ttacgagcct tttttaattt acatataaat tcattgtagg
     6661 tttattcagt ctatacatct tttcagttgc tgctcccata catacctctg agagcttgcc
     6721 atccaagtgg ttgcacgctt ctaatgcaga ggattccttc atatcagaca atttttctat
     6781 aactccttca acacacataa ctttatcctc ataatcacct tgattacaaa gttttgtaat
     6841 attagaaggc tttctggata cactaactat gtacattaat cccagcccat gaaaacatcc
     6901 tcttctttca atatcaggaa gattcataca tttgtttatc aattctgttc tatctatctt
     6961 taatttagat cttatcaagg gaaacatata cctataacat gcagctggat atctgttata
     7021 tttatcacac gggtaaaaat ctgtaatatc ttcattcata ttcatttcag attcatgaga
     7081 atgatgatgg tggtgtgaat gttcattctt ttgattagtc gatacaggaa gtttttgcct
     7141 cttatttctg aggttatctc tatactccat aaacacccct gtggcacagt agtagctaaa
     7201 ggatttatct gtaaatgtat cacaagcatc tattgatttt ctaatatcat gaccagaaac
     7261 aatcataaaa gcatgaccca gaccgtgcat acagttaccc ttcttatgaa gtttattcat
     7321 ttgttctgat tcacagaatg ttttggataa ggaaataatc tcttcaatgc ttttacttcc
     7381 aaatgcttct gttaccacac catgcataca tgcatttgta catctgttcc cgcaaactgc
     7441 aagcgactta ttaatatctt ttatcctgga gtaagttact tttcctacat catgtgcctg
     7501 actatggcaa agagtgtact tttcctcaag aaattccaac attgcaacgg gccctataat
     7561 atcataatac ttctcatata gaggcctttt agtacgagta tcattatatt gatttttaaa
     7621 ttcttgctgt aacgcgctat ctatattcac ccacttttta aaatccaatt gctcttgtga
     7681 atacatctca ttttgtgcat cagtaggcaa ctctgcagaa ttatcctgaa ccttttttgt
     7741 acaagaacta aagaacaggc taacaaacaa gactgtaaac aatattttca tttttttata
     7801 tttcatatca attaccacat acagactcca gggttattaa ccggaactgc tctaaaaccg
     7861 aggaactttc tttgtattag tgatgcagta gaaagttccg gatcattctc tgcgtttttt
     7921 atattaaaca gtttaccatt cctttcaaat tcaatttcga tatttttaac acctctttct
     7981 gatacctctg taatgatatt agctagatgc ttataagtta ttgcaaaata atatttttct
     8041 ttaacagacc tatctcttgt ttcctgatat cttatataat ttttaggcaa taatatttgt
     8101 gtttgtctta taggcctggt tacaacaggt ttagaaaatt tatccaaacc tcccagactt
     8161 gagcttttaa tatgaacaac attgcttaag ttatcaaata tctgaataga ggcaggaatg
     8221 aaaaaatgat tggatttact attttcagta tttagattac taaacatggt aaatacctga
     8281 atatttttaa ctcccaagta tgggaataaa cccactgtga aaagaataat aggcagtatt
     8341 actaatgctt tattagatgg aataaatctt agattgttac tattactttt acctttatag
     8401 aatctaatta agtaataaac aaacaaaata aaaaacacgc aaaatgagag catataaaac
     8461 ccctctctgc tgaatagaaa tattcttaga tcagtaccac ccatcataaa tgaaataaaa
     8521 ttgagtaata tcaataaggc tataagctgg actccgtaaa tatatttatt attcatatta
     8581 at
//
LOCUS       DBNGSV010000221         3535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1839494, whole genome shotgun sequence.
ACCESSION   DBNGSV010000221 DBNGSV010000000
VERSION     DBNGSV010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1839494"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="AC159U_08035"
     CDS             complement(<1..1551)
                     /locus_tag="AC159U_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3337047.1"
                     /translation="MSKAKKLSPYEIEIFNNILGSIAEEMGTMLIRAAYSPNIKERRD
                     LSCALFEENGEMVSQAAHIPVHLGSMSFAVKSVLSELDLSEGDVLIVNDPYRGGTHLP
                     DITIIAPFFYKNKIEYFLASRAHHADVGGITPGSMPLSKSIYEEGVIIPPSKLYSRGK
                     INRKLFNNIINSTRDPEERKGDFNAQIGAVNLGLKRLREVVDKYSLSKIRAASDQLLE
                     YSERMMRVVVKDIENGSYEFADYLDGDGFSPKKIKIKVKITIKGSKATVDFSGTDKQV
                     EGCLNCPLSVTTSAVLYVFQCLAPKELPLNSGSLRPVKIVTEKGKVIDAEYPSAVAGG
                     NVETSQRIVDVVFGALSKAIPDKVPAASAGTMSNVTFGGRVRKGNGKFAYYETIAGGM
                     GARNGMDGISAVQTHMTNTLNTPIESLERELPILVDSYSIRKNSGGKGRYSGGDGIVR
                     GYKFLTDVTVTLITDRRKTSPYGIKGGANAKPGRNSVVINGRKKALPAKTTMSLKKGS
                     VLTIETPGG"
     gene            complement(1548..2498)
                     /locus_tag="AC159U_08040"
     CDS             complement(1548..2498)
                     /locus_tag="AC159U_08040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3337048.1"
                     /translation="MKILLIRRGALGDTIVTLPLIQTLNEKFRNCHIEVIGEKSYWSL
                     IYPDYINKITASDSKYINSLYLEQSLDEEIVQYYKEFDLIFGFLLDREGTVKRHFKDM
                     DISNYSFKEPFTEKVGEHIVQYTTSILDEAGISYDDNKAPKIDIERSGVVYAEKLFNE
                     LGCNEKLISIHPRTYGIKGLTLDKFIELGRWVEDELSGIPIWILGPVEQDIREELESC
                     FNRDTVISENDLRKVAAIISATDFYVGCDTGITHLAAAAGVNTISIFGPTNPDIWGSL
                     GNNVKIIKTDNLDTFDNGIVKQLIINNINSGITKREIIQA"
     gene            2579..3529
                     /locus_tag="AC159U_08045"
     CDS             2579..3529
                     /locus_tag="AC159U_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3337049.1"
                     /translation="MKVIVTGAAGFIGSTLTEKLISQGFSVIGIDSVNDNYDVKIKNS
                     NISGLLKNKNFKFINSNILDIDFGGKYSEIQAIFHQAATAGVRSSWGKNFRDYTENNI
                     MATQHLLESFKDSGLKKFIYASSSSVYGNADEMPLRISTLTKPVSPYGVTKLAGENLV
                     NTYHANYGLPTVSLRYFTVYGPRQRPDMAFHIFMKSALQNKQIDIYGDGEQIRDFTYI
                     DDVVSANMLALDNGKNGSIYNIGGGSNISINKVLELIEHISGNKLDINYQQTQKGDVR
                     QTQADISLSKKELKYVPGFDIKTGLKNEYEWIKENIDLLT"
BASE COUNT          983 a          780 c          662 g         1110 t
ORIGIN      
        1 tccccccgga gtttcaatcg tgagcacact gccttttttc aaactcatag tagtctttgc
       61 aggaagagct tttttacggc cattaatgac aacactgttc ctgccgggct ttgcgtttgc
      121 cccgccctta atgccgtagg gtgatgtctt ccttctgtct gtgataagtg taacagttac
      181 gtcggttaaa aacttatacc cgcggacaat gccgtctcca ccgctgtacc tgcccttccc
      241 tccgctgttc tttctaatgg aatatgaatc aacaaggatc ggaagctctc gctcaagaga
      301 ttcaatcggg gtattgagtg tgtttgtcat atgagtctga actgcgctta tcccgtccat
      361 tccattcctg gcccccattc cacccgctat ggtctcatag tatgcgaatt tgccgttacc
      421 ctttcttacc ctgccgccaa atgtaacatt actcatggtt cccgcgcttg cggcaggtac
      481 cttgtcgggg attgcttttg ataaagctcc gaatacaaca tcgactatcc tctgtgaagt
      541 ctctacattt ccccctgcta cagcagacgg atattccgcg tctatgactt tccccttttc
      601 tgtgactatc tttaccggcc gcagcgagcc tgagtttagc ggaagctcct ttggagccag
      661 gcactgaaag acgtagagta cggcagacgt ggtaacactt agcggacagt taagacagcc
      721 ctcaacctgc ttatccgtac cgctaaaatc aacagtggcc ttactgcctt ttatggtgat
      781 ctttactttt atcttaatct tcttagggct aaagccgtct ccatccagat aatcagcaaa
      841 ctcataactg ccgttttcaa tatccttaac caccaccctc atcatcctct cgctgtattc
      901 caaaagctga tccgaggctg ctctgatttt agatagtgaa tatttatcca ctacttctct
      961 aagcctctta agacccagat taaccgcacc aatctgggca ttaaagtcac cctttctttc
     1021 ctcggggtct cttgtggaat tgataatgtt attgaatagt tttctattaa ttttgcctct
     1081 ggagtagagc tttgatggag gaatgattac cccctcttcg tatattgact tagatagagg
     1141 catcgagccc ggcgttatac caccaacgtc tgcatggtgc gcccttgagg ccaggaaata
     1201 ttctatttta ttcttataga agaacggagc aattattgtt atatcaggaa ggtgcgtgcc
     1261 ccctctgtat gggtcattta cgatcagcac atcaccctca ctcagatcaa gctcgctgag
     1321 tacggattta accgcaaaac tcatagagcc cagatgtacg gggatgtgcg ctgcctgaga
     1381 gaccatctct ccgttttctt caaaaagcgc acaggagagg tcccttcttt cttttatatt
     1441 aggagagtaa gcggcccgaa tcagcatcgt gcccatctct tctgctatgg agccaagaat
     1501 attattgaat atctctattt catatggaga gagtttttta gctttactca tgcttgtatt
     1561 atttcccttt tagtaattcc actattaata ttatttataa tcagctgttt tacaatcccg
     1621 ttatcaaatg tgtccagatt atcggtcttg atgattttta cattattacc aagtgacccc
     1681 catatatcag gatttgtggg cccgaatata gagatagtgt ttacccctgc tgcagctgca
     1741 agatgagtta tgcccgtatc acatcccaca taaaaatcag tagctgaaat tatggcggct
     1801 acttttctta ggtcattttc agatattaca gtatctctat tgaaacagga ctcaagctct
     1861 tctcttatat cttgctctac agggcccaat atccagattg gaattccact tagttcatct
     1921 tccacccacc tgccaagctc tataaattta tccagagtta gccccttaat gccatacgtt
     1981 ctgggatgga tggagattaa tttttcgtta catcctaact cattaaacaa cttctctgca
     2041 taaactacac ctgatctttc aatatcaatt tttggagctt tgttgtcatc ataggatata
     2101 cccgcttcat ccagtatgct cgttgtatat tgcactatat gctcacctac tttctctgta
     2161 aatggttctt taaagctata attggatata tccatatcct taaaatgcct ttttaccgta
     2221 ccttctctgt ctaataaaaa accgaagatg agatcgaatt ctttataata ctgtacaatc
     2281 tcttcatcca ggctttgttc taaataaaga gaatttatgt acttactgtc cgaagcggta
     2341 attttgttta tatagtcagg gtatatcagt gaccaatagc tcttttcccc gataacctcg
     2401 atatggcaat tcctaaattt ctcattgagt gtttgaataa gtggaagagt aacgattgta
     2461 tcacccaaag cccctcttct gattagcaat atcttcatct gtgtaaacct aatttacatt
     2521 gattattaac aaaaactata tatattcagg atttaataat ctaaaggtac aaaaaattat
     2581 gaaagttatt gtaactgggg cagcggggtt tattggttct actttaacag aaaaactaat
     2641 atcccaagga ttttctgtaa ttggaataga ttcggttaat gacaattatg atgtaaagat
     2701 taaaaacagt aatatttcag ggttgctaaa gaataaaaac tttaaattta ttaattccaa
     2761 tatcctggat atcgattttg gtggtaaata cagtgagata caagccatat ttcatcaggc
     2821 tgccactgca ggtgtaagat cgagctgggg caagaacttc agggattata ctgaaaacaa
     2881 tataatggcc acccagcatc tgcttgagag ctttaaagat tcagggctca agaaatttat
     2941 ttatgcttcg tcttcttcag tttatggcaa tgcagacgag atgcctctta ggatttctac
     3001 tctcacaaaa cctgtttcgc cttatggtgt caccaagctt gcaggcgaaa acctggttaa
     3061 tacatatcat gcaaactatg gccttcccac ggtttctctc cggtatttta ccgtttacgg
     3121 cccgaggcaa agacccgata tggcttttca tatattcatg aagtcggccc tgcagaacaa
     3181 gcagatcgat atctatggtg acggcgagca gatcagggac tttacctata ttgacgatgt
     3241 agtaagtgca aacatgctgg cattggataa tggtaaaaac ggaagtatat ataacattgg
     3301 tggagggagc aatatatcga ttaataaggt attggaatta attgaacata tttccggcaa
     3361 taagctagat attaactatc agcaaactca aaaaggggat gtgcggcaaa ctcaggccga
     3421 tatttcactg tccaaaaaag aacttaaata tgtgcccgga tttgatatta aaacaggctt
     3481 aaaaaacgaa tatgaatgga taaaggaaaa tatagattta ttaacctgaa tattc
//
LOCUS       DBNGSV010000222        13871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_524463, whole genome shotgun sequence.
ACCESSION   DBNGSV010000222 DBNGSV010000000
VERSION     DBNGSV010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_524463"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..344)
                     /locus_tag="AC159U_08050"
     CDS             complement(132..344)
                     /locus_tag="AC159U_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337050.1"
                     /translation="MKRKYARMFSRQVSDEGVKYMIDEIKDMSYEEALKINKENKDSI
                     WIPIPDGLYEKWVKNLYVGDKKLLKQ"
     gene            complement(715..1482)
                     /locus_tag="AC159U_08055"
     CDS             complement(715..1482)
                     /locus_tag="AC159U_08055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3337051.1"
                     /translation="MNYLDLAKKSALEAGELLLQYLGKITSYELKSPKSVLTEADTAA
                     EKLIINTIKSQYPDHGILAEESGDNVKGAKNIWIIDPLDGTTNFAHAYPFFSVSIALQ
                     IDGEVVVGVIYNPVKKELFSAHKGKGAYLNNNPISVSKVDDLELSHLVTGFVYEHDWM
                     VKKNLEYFEQFIYKSQAVRRDGSAALDLCYVACGRFDGFWEMGLNPWDTAAGVLILRE
                     AGGIVTNFSGTEFTLDSVELLGSNRLLHEVMLKILNI"
     gene            complement(1491..1868)
                     /locus_tag="AC159U_08060"
     CDS             complement(1491..1868)
                     /locus_tag="AC159U_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3337052.1"
                     /translation="MFALTADDDGKLLEFAYPLQFKGNVIPVDGKNIAGRVMISRRPY
                     LSNNVQKDKSHIFFSWLIGGKVLPVQKMIVYPVIFAERVIAILKIVKKGPDPETAGSD
                     FKRSDLKELDTIVNTVLTLHVVK"
     gene            2124..2570
                     /locus_tag="AC159U_08065"
     CDS             2124..2570
                     /locus_tag="AC159U_08065"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR3337053.1"
                     /translation="MKIAIGSDEKTALTDFVIQELNNRGIDTELYGPLAGTKEQWTDV
                     AELVARRVSGKNCDQGILFCWTGTGVSMAANKVAGIRAALCTDAQTAEGARKWNDANI
                     LAMSLRLITQAVAKEILDAWFSAEVDSSEQENIDKLDILEQKSKNI"
     gene            2585..3619
                     /locus_tag="AC159U_08070"
     CDS             2585..3619
                     /locus_tag="AC159U_08070"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3337054.1"
                     /translation="MSVKQYNIAIAGATGAVGVEMISILEQRNFPVKELTLLASERSV
                     GKCLPYKGEPIKVSELTEDSFKNIDIALFSAGSSRSLEFAPIAAESGAVVIDNSSAYR
                     MDADVPLIIPEINPEAISDYNKKNIIANPNCTTAVTIMGLKPIYDISKITRVVATSFQ
                     AVSGAGAQAIEELKAQTSAIVNSKEIKSEVFPHQIAYNVIPHIDSFTENGYTKEEMKL
                     HNETRKILGDDQIQLSATTVRVPVFRSHCISVNLETEHKVSIERAKEAFANFPGLSVL
                     DNPEKSEYPMPITTSGMDDCLIGRIREDFSVKNGISFWISGDQLRKGAALNAVQIAEL
                     LIKHIAKNNA"
     gene            3612..4160
                     /locus_tag="AC159U_08075"
     CDS             3612..4160
                     /locus_tag="AC159U_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3337055.1"
                     /translation="MPSILDKISNLLSSGQGSDPTAISDKLKDSIVSHTKEDEQLIQC
                     IKNHRAFHNSKNFNEKNMFFNSRCILTDRRLLIIRNLEYFKLFREINLLEIKSHRIEP
                     SDDDLVITLKTGRAEDVIEFSKHSLSWANEFSRVFEQTLLSAKEKYPVSQNGVPQKKC
                     SGCGKLVDQDSKFCPQCGNKLG"
     gene            4229..5104
                     /gene="dapA"
                     /locus_tag="AC159U_08080"
     CDS             4229..5104
                     /gene="dapA"
                     /locus_tag="AC159U_08080"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3337056.1"
                     /translation="MFEGSIVAIVTPFSRGKVDESALQKLVEFQIENGTHGIVACGTT
                     GESPTLSHEEHEHVIELIVKMVKGRVPVIAGTGSNSTDEAIRLTRFAKKVGADGSLLV
                     VPYYNKPTQDGLYSHFAGIADKVDIPIILYNVPGRTGVSLSPQTTAKLASSCKNIVGI
                     KDATGSVQTASKTLALTGLEFTLLSGEDAINFPILSVGGRGFITVTANIAPKEVADLY
                     NYYKKGKIDKARDLHYKLLELNEILFIESNPIPVKTALHLMGMVKNEFRLPLSKMAKD
                     NLAALKKSLKSYKLI"
     gene            5110..5706
                     /locus_tag="AC159U_08085"
     CDS             5110..5706
                     /locus_tag="AC159U_08085"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3337057.1"
                     /translation="MRYPDSSYEIPYWKDNKLVAGIDEAGRGPLAGPVVAAAVILPIQ
                     TDIPGINDSKKLSQKKRESLFDIIKEKSLSFAIGIVEPDEIDRINILNATLKAMYLAV
                     NQLTPIPNALLIDGINKINMNLDQNTITKGDSKCLSIACASILAKVTRDKIMEEYDKI
                     YYEYNFRKHKGYPTKEHLELIKRYGVCKIHRKTFRGTY"
     gene            5847..6191
                     /locus_tag="AC159U_08090"
     CDS             5847..6191
                     /locus_tag="AC159U_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3337058.1"
                     /translation="MKQDTAELRLPDKIYFRIGEVSDFTAIKPYVLRYWESEFPEIQP
                     IRRKSQRLYDRKAISTVLRIKNMLYDQNYTIAGAKKKLKEESNQRKGTSKATDLLHEL
                     LNDLKTVKDSLD"
     gene            6263..6338
                     /locus_tag="AC159U_08095"
     tRNA            6263..6338
                     /locus_tag="AC159U_08095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6296..6298,aa:Pro,seq:ggg)
     gene            6369..7136
                     /gene="surE"
                     /locus_tag="AC159U_08100"
     CDS             6369..7136
                     /gene="surE"
                     /locus_tag="AC159U_08100"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3337059.1"
                     /translation="MKKTILLSNDDGISSAGLNALAGALEPLGKTYIVAPDREQSASS
                     HALSIHRPLRIDKVTENSYSVDGTPTDCVNLAINGLLKDNKPDLVVSGINKGENLGDD
                     VTYSGTVSAAMEGTLLGIPSIALSLQGRNDFDFTASCTYSALIAEYVLECGLPKDILL
                     NVNIPNLDTDNIKGIKITRQGKRVYSDAVVEKKDPRGREYYWIGGDELGSVDLENSDI
                     DAVKNGFVSITPIKLDLTDYEYIKFLEKDLLKIKNHA"
     gene            7129..7716
                     /locus_tag="AC159U_08105"
     CDS             7129..7716
                     /locus_tag="AC159U_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3337060.1"
                     /translation="MLKRLYDWVIHWADTKYGVPAMAFVSFIESSFFPVPPDPLLLAL
                     SLGKPRKAIIYALICSVASIAGGVLGYLIGWTVWESVSELFFKYLFTQQTFDYVGIKY
                     SENAFLAILGAAFTPIPYKVFTISAGVFKINLAIFILASAIGRSARFLIEGVLIYLYG
                     ESIRNFIEKYLKWLSLLFFILLVAGIYVIKFVLHK"
     gene            7722..8210
                     /locus_tag="AC159U_08110"
     CDS             7722..8210
                     /locus_tag="AC159U_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HIR3337061.1"
                     /translation="MKARIGVIGPSQASTSEYKIAEEVGKEIALRDGVVICGGMGGVM
                     EAVSKGAKDQNGLVVGILPGDSSKAANKYVDIPIVTGFGQGRNIIVASSSEAVIAIGG
                     SFGTLSEISFALRLKIPVIGINTWNVSEQIIKAENPKQAVDKAFNSISHSNTTGMRHK
                     KT"
     gene            8101..8844
                     /locus_tag="AC159U_08115"
     CDS             8101..8844
                     /locus_tag="AC159U_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3337062.1"
                     /translation="MYQSRLLKQKILNKLSIKLLILFLIVTLQACGTKKPEPVLPAEP
                     PSTLSKKYIDTKVQGCIKEKSSYTFNKYHIMREGETLYRISKKYDVSVAKLIELNGIE
                     DHTDIEIGRLIRLPGSAKHAQYDWPLKGRLTSKYGKRGRRLHSGIDISADKGTHIKAV
                     ADGLVVESSNKIKGYSNYGRVVEIYHGSGITSLYAHNKKNLVKKGTCVRKGQSVAEVG
                     SSGNATGNHLHLEIRKDGKPVNPLIYLKQ"
     gene            8857..9378
                     /locus_tag="AC159U_08120"
     CDS             8857..9378
                     /locus_tag="AC159U_08120"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR3337063.1"
                     /translation="MKDLKKYVRDIPGFPKEGINFHDITPLLQNPKAFSFAVDTMAET
                     VSDKNIQYIVGVEARGFIFGSALAYKLDAGLVIVRKLGKLPSETINASYDLEYGEDVL
                     ELHKDSISHGENVLLVDDLLATGGTAAAVGELVNKLGGNIVGYLFLIELTSLNGREKL
                     KPYPVWSKLKYTE"
     gene            9404..10519
                     /gene="aroB"
                     /locus_tag="AC159U_08125"
     CDS             9404..10519
                     /gene="aroB"
                     /locus_tag="AC159U_08125"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3337064.1"
                     /translation="MQDKSNIIDVKIKGSKALSYQIEIGRGLFDKLAKELKEKELAYR
                     YAVVSDNNVSKLYYGKLQESLKNEGLSCLLITIPAGEQSKNRKNKEFIEDTMLKNGMA
                     RDSAIIALGGGVVGDIAGFVAATYNRGIPYIQYPTSLVACVDSSIGGKTAIDTEYGKN
                     LIGSFYQPVKVYVDLETLKTLKRQEITEGLAEVIKYGIIYDADFFHYIEKNINGIFNY
                     DTEALSNIVSRSCEIKGYVVENDEKESNLRKILNFGHTIGHAVEQLSNYKITHGNAIS
                     IGMVLEARVAVELGILDRIHLNEIIEIFSKAGLPTVLPEIIDKSKIVSVMKLDKKTRK
                     GNIEFAIPSEIGTMHSDNGSYGIKIDENIINKVIQNF"
     gene            10534..11160
                     /gene="trmB"
                     /locus_tag="AC159U_08130"
     CDS             10534..11160
                     /gene="trmB"
                     /locus_tag="AC159U_08130"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR3337065.1"
                     /translation="MKDLSYYRDYYIDLSVDLPPLNLKTIFNNTHEVSLEIGFGEGDF
                     LIELAMYHPLQNFIGLEIKKNRFKKAISHASSLGLKNIRFMHFDASIDLLQVFEQDIF
                     SQVFINFPDPWPKKRHQKHRIIKNDLLKQLYKITRQCCQIKIATDHKEYSDLILEEFR
                     DTDLYSLNSSLKYFGSYSKPQRTITKFERQFIKERREIYYLSYIKTNT"
     gene            11180..11413
                     /locus_tag="AC159U_08135"
     CDS             11180..11413
                     /locus_tag="AC159U_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337066.1"
                     /translation="MNLMLVCTILVITLSLTGCSAGNGAGHWFHILFVLIPFGITLYF
                     LNKKVDDISKSLFKIEGQLSLLATKADKSEKKK"
     gene            complement(11437..11973)
                     /locus_tag="AC159U_08140"
     CDS             complement(11437..11973)
                     /locus_tag="AC159U_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337067.1"
                     /translation="MYKIFKILIVGLLLFITTDAELVLSQDAGGAAAPAPAAPAPVAP
                     GGGALTAPLQFPEYVVTPKQDLNQFENSKVRKTTTEYREGSSTIGRSHTKKSNAEINA
                     EIERRRAEREEAEIQEAGIDAEVTEPDAVTEDYSGDLNVTGKSKLFKWKDKDGILHVT
                     DDPSAVPPEYKDQVLNSN"
     gene            12164..13183
                     /gene="aroF"
                     /locus_tag="AC159U_08145"
     CDS             12164..13183
                     /gene="aroF"
                     /locus_tag="AC159U_08145"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3337068.1"
                     /translation="MIVVMKNGAKKEQIEKVKSTIIEIGYKPHAIKGVLKSVIGVVGD
                     DRGKVNHLERLSLLEGVEKVVPISQPYKLASREITEQTSRFRVDKATVGGSKICVIAG
                     PCSVENEEQLITTAKAVKKAGATMLRGGAFKPRTSPYSFQGLGEEGLKLLAKAKEITG
                     LPIVTEIMDTDDLDLLEDYTDVLQIGARNSQNYSLLRRVGKSKKAVLLKRGMSTTINE
                     FLMCAEYILSEGNEKVMLCERGIRTFETATRNTFDLNAIPVLKEKTHLPVFADPSHGT
                     GYWQYVTPMALAAIAAGADGIAIEVHNNPEVAVSDGGQSLKPKKFAELMKKAAPVAKA
                     VGRKL"
     gene            complement(13180..13785)
                     /gene="purN"
                     /locus_tag="AC159U_08150"
     CDS             complement(13180..13785)
                     /gene="purN"
                     /locus_tag="AC159U_08150"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3337069.1"
                     /translation="MSQKTKIGVLVSGSGTNLQAIIDSNIPSCEITIVISNKPDAYAL
                     NRAEKHNIPAEVINHKDYDSREAFERQLIKCLDEKGVELIVLAGFMRVLTPFFVKHYK
                     HRIINIHPALLPSFPGVNSTKQAFDYGVRFTGCTVHFVDDGVDTGPIILQSVVEITPD
                     DTEDTLLEKIHEQEHIVFPKAVRLFCDGKISIDGRKVIISD"
BASE COUNT         4686 a         2498 c         2698 g         3989 t
ORIGIN      
        1 ggcacttcca atacaaacat ttgtattagc ttgcttatct tactatttga tagttttgta
       61 ttcttaagta acttcttttg catagtttca gcttagtata ctttgctgtc ctatgcgagc
      121 tccttaattt attattgctt taaaagcttt ttatctccca catacaggtt tttaacccat
      181 ttttcataaa gtccatcggg tattggaatc cagattgaat ccttgttttc cttatttatc
      241 ttaagtgcct cttcgtacga catatccttt atctcatcaa tcatatattt gacaccctca
      301 tctgaaacct gccgagaaaa catcctcgca tattttctct tcatatcttt tcacttccaa
      361 tttgccagtt agaagtgata gggaatgaaa tattattttt catcatcctc cctctatcca
      421 tcctaacaat tctttatttg ttaataatta taatatatta caaatgataa ttcaagtagt
      481 ttcttagagt atgttaaaaa aagttacaaa attaagatta attcttggtt atattagtct
      541 gaatttggtt attttttcta tttcaagtag atatatttcg tgattcaaga aggataaata
      601 ctaattatta aagtaggtta tgaagaattg ccaattcata tttcctagta attaaaattt
      661 tcattcttta ttgtacagtt gcaagacaaa taatcttttt gttataggat ttccttaaat
      721 attaaggatt ttaagcataa cctcatgaag aagcctgtta cttccaagaa gctctactga
      781 atccagagtg aattcagtgc ctgaaaagtt tgtaactatt cccccggctt cccttaatat
      841 aagcactccc gctgccgtat cccaggggtt aagacccatc tcccaaaacc catcgaaccg
      901 cccgcatgct acataacaca ggtcaagggc tgccgaaccg tctcttctaa ccgcctgtga
      961 tttatatata aactgttcaa aatactcaag attttttttc accatccagt catgttcata
     1021 tacaaatcct gttacaaggt ggcttagttc aaggtcatca accttagaaa cagatatggg
     1081 attattgttt aaatatgccc ccttaccctt atgggcgctg aataattctt tttttacagg
     1141 attatatatc acacctacta caacctctcc atctatctgc agagcaatgg agacagaaaa
     1201 aaacgggtat gcatgtgcaa aattagttgt gccatcaagc ggatctatta tccatatatt
     1261 cttagcgcct ttaacattat cgccggattc ttctgctaga attccatgat caggatattg
     1321 agactttatt gtgttaataa taagcttttc ggctgcggta tccgcttcag taagcacgct
     1381 ctttggagat ttaagttcat agcttgtaat cttaccaaga tattgcagta acagttcgcc
     1441 ggcctcaaga gctgacttct ttgctaaatc aagatagttc atgtagaggg ctattttacc
     1501 acatgaagtg ttaagacagt attaactatt gtatccagct cttttaggtc agaccttttg
     1561 aagtcacttc cggcggtttc cggatcaggg ccttttttta ctatttttag tattgctatt
     1621 acacgttcag caaatattac aggatagact atcatttttt gtaccggcag taccttaccg
     1681 ccaatgagcc aggaaaagaa tatatggctc ttgtcttttt gaacattgtt agagagatag
     1741 ggccttcttg agatcataac cctgcccgca atgtttttgc cgtctacagg aataacgttt
     1801 cccttaaact gcaatgggta ggcaaactca agcagctttc catcatcatc tgcggtaagg
     1861 gcaaacatgc tgtcttttgg aagctgaaat gcttttgata ttttcctgtc aagctctatg
     1921 taatcgatcc cggttatctc ctttgtataa ttaataaata ttatagataa attaaatttg
     1981 ggtcaataag actaatgaat aaaatagcat aaaacctgta aataataata tctctttatt
     2041 tcaggattta aaaatataga cttattatac tatgaatttt tataacttga tgtatataaa
     2101 ttgttattta agatatgaaa aaaatgaaaa tagcaattgg tagtgatgaa aaaacagctc
     2161 ttacagactt tgttatacaa gagcttaaca acaggggaat agacacagag ctttacgggc
     2221 ctcttgcggg tactaaggag cagtggacag atgtagcaga acttgtagcc cggagggtat
     2281 caggcaaaaa ctgtgatcag ggtatactgt tttgctggac cggcacaggg gttagtatgg
     2341 ctgcaaataa ggttgcagga ataagggctg ctctttgtac agatgcacaa accgccgagg
     2401 gtgccaggaa atggaatgat gcgaatatac ttgccatgag cctacggctt attacccagg
     2461 cagtagcaaa agaaattcta gatgcctggt ttagcgctga ggttgacagc tctgaacagg
     2521 aaaatattga taagttagat atactggaac aaaaaagtaa aaatatataa acgagataat
     2581 agaaatgagc gtaaaacaat ataatatagc cattgccgga gcaactggtg cagtcggagt
     2641 agagatgata agcatcttag aacaaaggaa ctttccagta aaagagctta ccctgcttgc
     2701 atccgagaga tcagtaggaa agtgcctgcc gtacaaaggt gagcccataa aggtttcgga
     2761 gcttacagaa gactctttta aaaatataga tatcgctctt ttttctgctg ggtcttccag
     2821 aagccttgag tttgctccaa ttgccgctga gtcgggagca gttgtaatag acaatagctc
     2881 agcatataga atggacgctg atgttccgct aataattcca gaaataaacc ctgaggctat
     2941 ttcagattat aataaaaaaa atattattgc taaccctaac tgcactacgg ctgtaaccat
     3001 aatggggcta aagcccatat atgatatatc aaaaatcaca agggtagtcg ccacgagttt
     3061 tcaggcagtt tcgggcgccg gagcgcaggc aatcgaagaa ctaaaagccc agacaagtgc
     3121 tatagtaaac tcaaaagaga ttaagtctga ggtatttcct caccaaatag cctacaatgt
     3181 tatccctcat atagacagct ttacagaaaa cggctacacc aaagaagaga tgaagcttca
     3241 taatgaaacc agaaaaatac tcggtgatga tcaaattcaa ctctcggcaa ccacggtaag
     3301 ggttccggta ttcaggtctc actgcatctc tgttaatttg gagactgaac ataaggtttc
     3361 catagagagg gctaaagaag cttttgcaaa tttccccggg cttagtgttt tagataatcc
     3421 ggaaaagtcg gaatatccga tgccgattac tacaagcggc atggatgact gtcttattgg
     3481 cagaatacgc gaagacttta gcgttaaaaa tggaataagt ttttggatct caggtgacca
     3541 gcttagaaaa ggtgcggcac taaatgcagt tcaaattgcc gagcttctca ttaaacacat
     3601 tgccaaaaat aatgcctagc atacttgata aaatatcaaa cctgctttca tcaggacagg
     3661 gttctgaccc cacagccatc tcagataagc ttaaagacag tattgtatca cacacaaaag
     3721 aagacgagca gcttatacaa tgcataaaaa atcacagggc atttcataac tctaaaaact
     3781 tcaatgaaaa gaacatgttt tttaactcaa ggtgtatcct tacggacaga aggcttctta
     3841 taatcagaaa ccttgagtat tttaagctct ttagagaaat taatttactg gaaataaagt
     3901 ctcacagaat agagccctca gatgatgatc tagtaataac actaaaaaca ggaagagcag
     3961 aagacgtgat tgaattttca aagcactcac ttagctgggc taatgaattt tccagggtat
     4021 tcgaacagac actgctgtct gcaaaagaaa aataccctgt atctcaaaac ggagtacctc
     4081 aaaagaaatg cagcggctgt ggcaagcttg tagatcagga ctccaaattc tgccctcagt
     4141 gcggaaataa actgggctaa catttcttgt cttgatttca cttcccacta aaatataatg
     4201 aagctttata aattaaagga actaaaaaat gtttgaagga tcaatagttg caatagttac
     4261 accattttca agaggtaaag tggatgagtc tgctctgcag aagctcgttg agtttcagat
     4321 tgaaaatggt acacatggga tagtagcatg cggaaccacg ggtgaatccc ccactctttc
     4381 tcatgaagaa catgagcacg taatcgagct tatagttaag atggtaaagg gcagagttcc
     4441 tgtaatcgcg ggaacaggat cgaactccac agatgaagct ataaggctta cccgttttgc
     4501 aaagaaagtt ggtgccgatg gatcgctgct tgttgtaccc tattacaaca aacccactca
     4561 ggacgggcta tacagccatt ttgcaggtat tgcagataaa gtcgatatcc cgattattct
     4621 ttataacgta ccgggaagaa ctggggttag cctaagccct cagacaactg ccaaactggc
     4681 cagctcatgt aaaaacattg taggaataaa agatgcaacg ggctcagtac aaaccgcatc
     4741 caagaccctt gctcttacag gtctggagtt tacgctccta tcgggagaag atgcaataaa
     4801 tttcccaatt ctttcagttg gcggcagagg ttttataaca gtaactgcca acattgctcc
     4861 aaaagaggtg gcagatttat ataattatta caaaaaagga aaaatagaca aagctagaga
     4921 tttgcattat aagcttcttg aattaaacga aattctattt atagagtcaa accctattcc
     4981 tgttaagact gctttgcacc tcatgggaat ggttaaaaat gaattcagac tgcctctttc
     5041 caaaatggca aaagataacc ttgcggctct taaaaaatct ctaaaatcct ataagcttat
     5101 ctaaaaatat tgagatatcc tgattcatct tatgaaattc catactggaa ggataataag
     5161 cttgtagccg gtattgatga agccggcaga ggcccgcttg caggcccggt tgtagcggca
     5221 gccgtaatat tgccaataca gacggatata ccggggataa atgattcaaa aaaactatct
     5281 caaaaaaaaa gagaaagttt atttgatatt attaaagaga aatccttatc ttttgcgatt
     5341 ggtatagttg aaccggatga aatagataga ataaatattc ttaatgctac actaaaagcc
     5401 atgtatttag ctgtaaatca gctaacccca atcccaaatg ccctgcttat tgatggaatt
     5461 aacaaaatta atatgaacct tgatcagaat actataacaa aaggtgattc aaaatgcctt
     5521 tctatagcat gcgcttcaat tcttgctaaa gtaaccaggg ataaaataat ggaagaatat
     5581 gataaaatat attacgaata taattttcgc aagcacaagg gctaccccac aaaagaacat
     5641 ttagagctta taaaacggta tggagtatgt aaaatacata gaaaaacatt tagaggaaca
     5701 tattaggttt agtacctaaa ttaatagatt ttttaaacaa tttaaaatta tgtattccaa
     5761 ttttctcaaa atgttgtaca ataaaaaaca gtttattagt gtgggttaat aaataaatgt
     5821 gggtttatta aataagagat aaagagatga aacaagacac tgcagaacta agactgcccg
     5881 ataagatata tttcaggatt ggagaagtaa gtgattttac agcaataaaa ccttatgttt
     5941 taagatattg ggaaagcgaa tttcccgaaa tccagccaat aagaagaaag tcccagcgcc
     6001 tttatgacag aaaagctatc tctacagtgc ttcgcataaa gaatatgctt tatgaccaaa
     6061 actatacaat agcaggcgct aagaaaaagc taaaagaaga atcgaatcag agaaaaggca
     6121 catccaaagc caccgatctg cttcatgaac tgttaaatga cttaaaaaca gtaaaagatt
     6181 ctctcgacta actaatatta attagttatc ttattccctg atttttcccc aaaatacagt
     6241 attggtatat agtttttccg ttcggggtgt ggcgcagttg gtagcgcacc ggtctggggg
     6301 accgggggtc atcggttcaa atccggtcac cccgaccaca tttttatagc tttatataaa
     6361 ataggctgat gaaaaagaca attctactct caaatgatga tggtataagc tcagccggac
     6421 taaatgcact tgccggagca cttgagcctc tgggaaaaac atacattgtt gccccagaca
     6481 gggagcaaag cgcatcaagc catgctctta gcatacacag gccgcttaga attgataagg
     6541 ttacagaaaa ctcatacagc gtagacggta cacctactga ctgtgtaaac cttgcgatca
     6601 atggtcttct gaaggacaat aagccagatc ttgtggtctc cggtatcaat aaaggtgaaa
     6661 accttggaga tgacgttact tattcgggca ctgtgagtgc agcaatggaa ggcacacttc
     6721 tgggaatacc ctccatagcc ttatctcttc aaggaagaaa cgatttcgat tttacagcat
     6781 cctgtacgta ttcggcgctt attgctgaat atgtacttga gtgtggtctt ccaaaagaca
     6841 tcttactaaa tgtaaatata ccaaaccttg atacggacaa tattaaaggg atcaaaataa
     6901 caagacaggg taagagagtg tatagcgatg ctgttgtaga aaaaaaagat ccgcgcggaa
     6961 gagagtatta ctggataggc ggagatgaac tgggctctgt agatcttgaa aactccgaca
     7021 tagatgccgt aaagaacggg tttgtatcaa tcacccctat taaacttgat ctaaccgact
     7081 atgaatatat aaaattttta gaaaaggacc tgcttaaaat aaaaaatcat gcttaaaaga
     7141 ctttatgact gggtaattca ctgggcagac acgaaatacg gtgttccagc aatggcattt
     7201 gtatcattta tagaatcctc tttctttcca gttcctcccg atcctcttct tcttgcgctg
     7261 agtttaggaa aacccagaaa agccataatc tatgcactaa tctgttctgt ggcatcaata
     7321 gcaggtggag tcttaggata cctgatcgga tggactgttt gggaatcggt aagtgagtta
     7381 ttcttcaaat atctttttac tcagcaaaca tttgattatg tgggtataaa atatagcgaa
     7441 aatgcctttc ttgctatatt aggtgcagct tttaccccta ttccgtataa ggtatttacc
     7501 atatcggcag gtgtatttaa aattaatcta gcaatattca ttctcgcatc tgcaattgga
     7561 agatcggcga gattcttgat tgaaggcgtg cttatatacc tgtatggaga aagtataaga
     7621 aactttatag aaaaatatct aaaatggctg tctttactat ttttcatatt gcttgtagca
     7681 gggatatacg ttattaaatt tgtccttcat aaataaatgt catgaaagca agaattgggg
     7741 ttataggtcc atctcaggca agtaccagcg aatataaaat cgcagaggaa gtaggcaaag
     7801 aaattgccct acgggacgga gttgttatat gtggcggtat gggcggggta atggaggctg
     7861 tatcaaaagg tgcaaaagat caaaacgggc ttgtagtagg tatactaccc ggagacagct
     7921 cgaaagctgc aaacaagtat gttgatatcc ccatagttac gggctttggt cagggtagaa
     7981 acataatagt tgcaagttca tctgaggcag ttatagcaat tggaggaagt tttggtacac
     8041 tttctgaaat ttcatttgcg ttaaggctta aaatccctgt aataggaata aacacctgga
     8101 atgtatcaga gcagattatt aaagcagaaa atcctaaaca agctgtcgat aaagctttta
     8161 attctatttc tcatagtaac actacaggca tgcggcacaa aaaaacctga gcccgtactg
     8221 ccagcagaac ctccttctac attatcaaaa aaatatattg ataccaaagt acagggatgc
     8281 ataaaagaaa aaagcagtta tacatttaat aaatatcata taatgagaga gggtgaaaca
     8341 ctttacagaa tatccaaaaa atatgatgta agcgttgcaa agttaataga acttaacggt
     8401 atagaagacc atacggatat agagattgga agattaattc gcttaccggg gtctgccaag
     8461 catgctcagt atgattggcc gctcaaagga aggttaacct caaaatatgg caaaagaggc
     8521 agaagattac acagcggtat tgatatatca gcagataaag gtacacatat aaaagctgtt
     8581 gcagacgggc ttgtagttga aagcagcaac aagattaagg gttatagtaa ttacggacga
     8641 gtggttgaaa tttatcatgg aagtggaata acctcattat atgcgcacaa caaaaaaaat
     8701 cttgtaaaaa aaggaacttg tgtaagaaaa ggccagagtg tggcagaagt aggctcatcg
     8761 ggaaatgcca ccggaaatca tctgcatctg gaaataagaa aggatggcaa accggttaac
     8821 ccgctaatat atttaaagca gtaaggagga tgaactatga aggatcttaa aaagtatgta
     8881 agagatattc cgggatttcc aaaagaaggg attaattttc atgatataac tcctttactc
     8941 caaaatccta aagctttctc ttttgccgtt gatacaatgg ctgaaactgt ttcagacaaa
     9001 aacatacagt acattgttgg agtcgaagcc agggggttta tatttggatc agctcttgca
     9061 tataaactgg atgcgggtct tgtaatagta agaaaactcg gaaagcttcc ttctgaaacc
     9121 ataaatgcct cttatgacct cgagtatgga gaagatgtgc ttgagctgca taaagattca
     9181 atttctcatg gtgaaaacgt tttactcgtg gatgatctcc ttgcaacagg gggaacggcc
     9241 gcagcagttg gagagctcgt taataagctt ggaggcaata tagtaggcta cttgttttta
     9301 atcgagctta cctcactaaa cggaagggaa aaactaaagc cctaccctgt atggtcaaaa
     9361 ctaaaatata ctgaatgata ttttctatag ataactacaa tatatgcagg acaaatccaa
     9421 tatcatagac gttaaaatta aaggttcaaa agcgctatcc taccaaatag aaataggaag
     9481 aggacttttt gacaagcttg ccaaagaatt aaaggaaaaa gaacttgcat acaggtatgc
     9541 agtggtatct gacaacaacg tatcaaagct ttattatggc aaactgcaag aatcattaaa
     9601 aaatgaaggt ttatcatgcc tgcttataac aattccagca ggagaacaat caaaaaacag
     9661 gaagaataag gaatttattg aagatacgat gcttaaaaac ggcatggcga gagactctgc
     9721 aataatagcc cttggcggag gagttgttgg tgatattgcc ggatttgtag cggcaactta
     9781 taacagggga attccatata ttcagtatcc aacttcactt gttgcctgtg tggatagttc
     9841 cattggaggt aaaactgcaa ttgatactga atatggaaag aatctcattg gttcatttta
     9901 tcagcctgtt aaggtttatg ttgacttaga aactctaaaa actctaaaga gacaggaaat
     9961 aacagaagga ctggcagaag taataaaata cggaattatt tatgatgcgg atttttttca
    10021 ctatatagaa aaaaatataa atggaatatt taattatgat acagaagcac tcagcaatat
    10081 tgttagcaga agctgtgaaa taaaaggata tgtagtagaa aatgatgaaa aggaatctaa
    10141 cttaagaaag attctaaatt tcggccatac tataggacat gccgttgaac agctatcaaa
    10201 ctataaaatc acacatggaa atgcaatttc tataggcatg gttttagaag ctagagttgc
    10261 tgttgaactg ggcatattag atagaattca tttaaatgaa ataatagaaa tattttctaa
    10321 ggcaggcctt cccactgtac ttccggagat tatagataaa tctaaaattg tctctgtaat
    10381 gaagctcgac aaaaaaacaa ggaagggaaa tattgagttt gcgatacctt cagagattgg
    10441 aactatgcac tcagataacg gaagttatgg aattaaaatt gacgaaaaca ttattaataa
    10501 ggttatacaa aatttttaat aaatcttgcc tggatgaaag atctatctta ttaccgcgac
    10561 tattatattg atctttccgt tgatcttcct ccgctaaatc tcaaaacaat atttaataac
    10621 acccatgaag tatcattaga aataggtttt ggtgaaggag attttctaat agagcttgca
    10681 atgtaccacc ctttgcagaa ttttataggc ctggaaatta aaaagaacag gtttaaaaaa
    10741 gcaataagtc atgcaagcag ccttggactt aagaatatca ggttcatgca ttttgatgcc
    10801 tcaatagatc tacttcaggt ttttgaacaa gacatctttt ctcaagtttt cataaatttt
    10861 cccgacccat ggccgaaaaa gagacaccaa aaacacagga taataaaaaa cgatcttctg
    10921 aaacaattat ataagataac aagacaatgc tgccaaataa aaattgcaac agaccataaa
    10981 gaatattcgg atttaatatt agaagaattt cgcgatacag acttatacag tctaaattcg
    11041 agtttgaaat attttggttc ttattctaaa ccacaaagaa ctattaccaa atttgaaaga
    11101 cagtttataa aagaaagaag agaaatttat tatttaagtt atattaaaac aaacacttaa
    11161 aataatcagg agatacgaaa tgaatttaat gttagtttgt acgattcttg taataacgtt
    11221 atcacttacg ggttgcagtg ctggaaacgg tgcggggcat tggtttcaca tactatttgt
    11281 gcttataccg tttggtataa ctctatactt tttaaataaa aaagtggatg atataagcaa
    11341 atcactattt aaaattgaag gacagctatc actgcttgct actaaagctg ataaatctga
    11401 gaaaaagaag tgagatagcg cagcaataaa actattttag ttactgttta atacctgatc
    11461 cttatactca ggtggtactg ctgatggatc gtcggtaaca tgtaatattc catccttatc
    11521 cttccattta aacagctttg acttacccgt gacattaagg tctccggaat aatcttctgt
    11581 aactgcatca ggctcggtaa cttcagcatc aataccagct tcttgaattt cagcttcttc
    11641 acgctctgct cttcttcttt caatctctgc atttatttca gcattgctct ttttagtatg
    11701 agatcttcca attgtgcttg aaccttccct gtactcagtg gtggtttttc ttacttttga
    11761 gttttcaaac tgattgagat cttgtttagg agtaacaaca tattcgggaa actgcagagg
    11821 agccgtgagt gcacctccac caggggctac tggtgccgga gctgctggtg ccggagctgc
    11881 tgctccgcct gcatcctgtg aaagtaccaa ctcagcgtca gttgtaataa atagtaatag
    11941 acctacaatc aatattttaa atattttata cataacttta ttttcctccc ataattaact
    12001 ataatcataa tacttttatt atgttaatta taactatata atagctcaga ttgttacgag
    12061 tatcaataat ttatattaac aattcacata tgttgacaag aaacattgat tatgtaatat
    12121 gggcaattta tacaactaaa cacaaagcga gggaaattac acaatgattg tagtaatgaa
    12181 gaatggggca aaaaaggaac aaattgaaaa agttaaatcg acaattatag aaatcgggta
    12241 caaaccacat gcaataaaag gggttcttaa gtcagtaata ggagtagtgg gagacgacag
    12301 aggaaaggta aatcacctgg agaggctaag cctgcttgaa ggagtagaaa aagttgttcc
    12361 gatttctcag ccctacaaac ttgcaagccg tgagattacc gaacaaacct caagatttag
    12421 agtagacaaa gcaactgttg gaggcagcaa gatatgcgta atagccggcc catgctcagt
    12481 agaaaatgag gagcagctta taaccactgc aaaagctgta aaaaaagcag gcgcaacaat
    12541 gttaagaggt ggtgcattca aacccaggac ctctccatac tcattccagg gtcttggtga
    12601 agaaggtcta aaactcctgg ctaaagcaaa ggaaataact ggccttccaa tcgtaacaga
    12661 aattatggat accgatgatc ttgacctcct ggaagattac acagatgtac ttcagattgg
    12721 cgctcgcaac tctcaaaact attcacttct aagacgagtt ggaaaatcca aaaaagcagt
    12781 tcttctcaaa cgtggaatgt caactactat aaatgagttc ttaatgtgcg ctgagtatat
    12841 actttctgaa ggaaacgaaa aagttatgct ttgcgaaaga ggaattagaa catttgaaac
    12901 agctacgaga aacacatttg atctaaatgc aattccggtg ctaaaagaaa aaactcatct
    12961 acctgttttt gctgatccaa gccacgggac aggctattgg caatatgtta ctccaatggc
    13021 acttgcagct attgcagcgg gtgcggacgg cattgctata gaggtccaca ataatcctga
    13081 ggttgctgtt agcgatggtg gtcagtcctt aaaacccaaa aagtttgcag aacttatgaa
    13141 aaaagcagca cctgtagcaa aagcagtagg cagaaagctt taatcggaaa taattacttt
    13201 ccggccatct atagagattt tcccatcaca aaatagtctc acagcctttg ggaaaactat
    13261 atgttcctgt tcatgtatct tttctaataa agtatcttca gtatcgtccg gtgtgatttc
    13321 tactacagat tgtaaaataa taggcccagt gtcaactccg tcgtctacaa agtgtactgt
    13381 acatccagtg aacctaacac cataatcaaa ggcttgtttg gttgagttca ctcctggaaa
    13441 tgaagggaga agagcgggat ggatatttat tattctatgt ttatagtgct ttacaaaaaa
    13501 aggggtaagc actctcatga agcctgcaag cactataagt tctactccct tctcatcaag
    13561 acatttaatt aactgccgct caaatgcttc cctgctatca tagtctttat ggtttataac
    13621 ttcggcaggg atgttatgtt tctcagctct attaagagca tacgcatctg gtttattgct
    13681 tattacaatt gtaatctcgc atgagggtat gttactgtct ataattgcct gcagatttgt
    13741 gccgctacca gatactaaaa ctccgatttt agttttttga gacatattct ggataataac
    13801 agaacaattc ttattttcta gtttggtcga gggaatttaa ggaagaaaaa aaaagaatta
    13861 gggcagctac c
//
LOCUS       DBNGSV010000223         3951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1770811, whole genome shotgun sequence.
ACCESSION   DBNGSV010000223 DBNGSV010000000
VERSION     DBNGSV010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1770811"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="AC159U_08155"
     CDS             <1..1306
                     /locus_tag="AC159U_08155"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3337070.1"
                     /translation="TQRHALFDIFLTDSLLRLSYHIVVGKVDPERLDPNWNLAYDLNY
                     TVPEVTIQQVLDSHNIIGFIDDLKPDHFYYLRMKKALAHYRDLKHKGGWNVVSVGPTL
                     KLGMTDNRVVELRKRLLVTADLEAYIPEDGRTFDTKLEQGVKEFQRRHGLIVDGLVGK
                     NISKALNVSVDQRIDQIRVNIERARWVLRNLPPEFIIINIAGFTVFLVKNGESVWRSK
                     VVVGKTYRKTPVFKAEMMYIDFNPTWTVPPIILRNDILPEVKKDPVYLQKKRIRVLSK
                     TGQEIPQDTINWSRYSALNFPYILRQDPGPDNGLGVVKFMFPNKHFVFLHDTPSKSLF
                     EKDIRTFSSGCIRVQNPLELAELLLSDQEYWNREAIEKVVAWGKTKTVHLKKPIPVLV
                     LYWTSRVDQEGKVQFLPDVYNRDQEVLRELDAEFKVRKRDVQ"
     gene            1521..2066
                     /locus_tag="AC159U_08160"
     CDS             1521..2066
                     /locus_tag="AC159U_08160"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3337071.1"
                     /translation="MKYLFILFSLFFVTLGNAAQVDLKGSEFGWYGSKIGGEHFGKIM
                     IKEATLNETYGKVTGGEFVMDMSSITVENIDSPKYEKKFLDHMKSIDFFDTANYPTTT
                     LKINKIDGNNVEGTLTIRGKTHPVAFPIERKGEIVFGKMTFDRTKYDIIYKSKNFFEN
                     LGDKIIHDEVVLNFKVKLVEG"
     gene            complement(2155..2490)
                     /locus_tag="AC159U_08165"
     CDS             complement(2155..2490)
                     /locus_tag="AC159U_08165"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3337072.1"
                     /translation="MDKVNKKRASRRLPYQNEIELIGNAGQYCRDTLINISETGVCIS
                     TDIDIPAGTETISRFLVDSEKLCELEGRVAWCTKNQDNKTFRIGIELVKTPDDFNEVY
                     ESIGKKFGL"
     gene            complement(2651..3487)
                     /locus_tag="AC159U_08170"
     CDS             complement(2651..3487)
                     /locus_tag="AC159U_08170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3337073.1"
                     /translation="MYKYIYILPLVLYLYLLPLISAEKVTISIQPENLYPGDVFVINI
                     DSHNIPSALFQGQSINFYLSEGIYKALCFIDINTMPGNYPLNINNGDITKTYNIKVKH
                     KVFPVKQITLKSEKVFLSPEDEKRANEESALLKSIWNEVTPNPLWKGQFIRPIDSEIT
                     SQFGVKRIINKKKESRHRGTDYRGKTGTPIKSINSGIVELTDNQFYGGNTVVINHGLG
                     LYSVYLHLSKTSARSGDQINKGDIIGLVGSTGRASGPHLHLSVKLYGESINPESLYEL
                     DL"
     gene            3510..>3951
                     /locus_tag="AC159U_08175"
     CDS             3510..>3951
                     /locus_tag="AC159U_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3337074.1"
                     /translation="MNKKVYLDYNATTPVDPRVLEVMIPYMKDMYGNPSSVHSFGGDS
                     KAALDDAREQVAELIGARQRDIYFTTGGSESNNFAIKGIAYQLKDKGRHLITTQVEHA
                     SVLETFKYLESEGYQVIYLSIDRDGMLDVDELRDVICEDTILVSI"
BASE COUNT         1301 a          647 c          748 g         1255 t
ORIGIN      
        1 cacgcagagg catgctctgt ttgatatttt tttaacagat tcgctgctgc gtcttagtta
       61 ccatatagtt gttggtaaag tagacccgga gaggctcgat cctaactgga atcttgccta
      121 tgatttaaat tatactgtgc cagaggttac aattcagcag gttcttgatt ctcacaatat
      181 catcggattc atagatgact tgaagcccga tcacttttat tacctgagaa tgaaaaaagc
      241 gcttgctcac tacagggatt taaaacataa gggcggctgg aatgtagtat cggtaggtcc
      301 tactctaaaa ctcgggatga ccgataacag agtggttgag cttagaaaaa ggcttcttgt
      361 cactgccgac ctagaagctt atatcccaga agatgggaga acatttgata ccaagcttga
      421 gcagggtgta aaagagtttc aaagaaggca tggtctaatc gtcgatggac ttgtaggtaa
      481 aaatatttcc aaagccctca atgtttcagt agaccagaga atagatcaga tacgtgtgaa
      541 tatagaaaga gcaaggtggg tactccgaaa tctacccccg gaatttataa tcataaacat
      601 agccggcttt acggtgttct tagtaaaaaa tggcgagtcg gtctggagat ctaaggtagt
      661 agttggcaaa acataccgca aaacacctgt atttaaggca gagatgatgt atatagattt
      721 taatccaacc tggaccgtac cacccataat actcagaaat gatatactgc cagaagtaaa
      781 aaaagatccg gtttatctcc agaaaaaaag aattagggta ctgagtaaga ctggccaaga
      841 gatacctcag gacactatca actggtctcg gtattctgct ttgaattttc catatatttt
      901 gagacaagac cctgggccgg ataatgggct tggtgtggtt aagtttatgt ttccgaataa
      961 acactttgtt tttcttcacg atacccccag caagtcctta tttgaaaagg atattaggac
     1021 ttttagctcc gggtgtataa gggtgcaaaa tccccttgaa cttgccgagc tgttactgag
     1081 tgatcaagag tactggaata gagaagcaat agaaaaagtg gtagcctggg ggaaaaccaa
     1141 aaccgtacat ttaaaaaaac caatcccggt tcttgttctc tactggactt ccagagttga
     1201 ccaagaagga aaggttcagt ttttaccaga tgtttacaac agagatcaag aggttttgcg
     1261 tgaattagat gcagagttta aggtaagaaa aagagacgta cagtaattgc cttacgatct
     1321 ttcattgctt tataatatct gaacacagat acataaatcc atttctttgc ccctttacct
     1381 aaaaaataag tggacaagaa agttagtatc aagaattatt gatcgttgaa ccgtgattta
     1441 caagctctat taataaatgg ttgaaaattg aaatattttc gttaactttt aattcaataa
     1501 aattattggg aggaataact atgaaatatc tatttatact ttttagcctg ttttttgtaa
     1561 cattaggaaa tgctgcccag gttgatctca agggaagtga gtttggatgg tatggctcaa
     1621 aaatcggggg tgaacatttt ggcaaaataa tgattaagga agcaacatta aacgaaactt
     1681 acggaaaagt aactggcggc gagtttgtta tggatatgag cagtattact gttgagaata
     1741 tcgattctcc aaagtatgag aagaagtttt tagatcacat gaaaagcatc gatttttttg
     1801 atacagctaa ttatccaaca acgacgttaa agataaacaa aatagatggt aataatgttg
     1861 aaggtacttt aaccatcagg ggtaaaactc atcctgtagc ttttccaatt gagaggaaag
     1921 gtgaaatagt ttttggaaaa atgacttttg accgcacaaa atatgatatt atatataaat
     1981 caaaaaattt ctttgaaaat cttggtgata agatcattca tgatgaagta gtacttaatt
     2041 ttaaggttaa gcttgttgag gggtaagttt attaacttac actttcagaa taggatcaat
     2101 attattcgac atcaggggtt aaaaattaat ggataggtgc ggttttagat aattttacaa
     2161 accaaacttc ttaccaatac tctcatatac ttcattaaaa tcatctggtg ttttaaccag
     2221 ttcaatacct attctaaagg tcttgttatc ttgatttttt gtacaccatg ctactctacc
     2281 ctcgagttcg caaagttttt cagaatcaac aagaaatctg gatatagtct ctgtacctgc
     2341 aggaatatca atatctgttg aaatgcaaac acctgtctcg gatatattaa taagtgtgtc
     2401 cctgcagtac tgaccggcat tgccaattaa ttcaatttca ttttgatatg gaagccgtct
     2461 ggatgctctt tttttattga ccttatccat aacttatatc cgattataaa gtatatagta
     2521 aattcctcta catttacaac aaatcaaaat aagtaacaaa ttatttctat tataaatatg
     2581 taaaacttaa tgtattttct aataaatatt atatctatct atgtatagat agatataatt
     2641 caaatgatta ttacaaatct aattcataaa gcgattctgg attaatgctc tcaccataaa
     2701 gcttcacgct taaatgcaga tgcggaccac tcgcacgacc tgtactgcca acaaggccga
     2761 ttatatcccc cttatttatc tgatcaccac ttctagcact tgtttttgat aaatgtaaat
     2821 aaactgaata aagaccaaga ccatgattta taacgacagt gttaccacca taaaattgat
     2881 tatctgttaa ttcaacaatt ccgctattaa ttgattttat aggtgtgcct gtttttcctc
     2941 tataatctgt acccctatgc ctactttctt tctttttatt aattatccgt ttaacaccaa
     3001 actgagaggt tatttcgctg tctataggtc taataaactg gcctttccat aatggattgg
     3061 gtgtgacctc attccagata gatttaagaa gagcagattc ttcattggct cttttctcat
     3121 cctccggact aaggaatact ttttcagatt ttaaagtaat ttgctttaca ggaaatactt
     3181 tgtgcttaac ttttatattg tatgtctttg taatatcgcc attattaata tttaggggat
     3241 agttaccggg cattgtatta atatcgataa aacaaagtgc tttataaata ccctcgctta
     3301 aataaaaatt tatagattgc ccctgaaaca aggcacttgg tatgttgtgt gaatctatgt
     3361 ttattacaaa gacgtctcca gggtataggt tttcaggctg gatagatata gttacttttt
     3421 cagcagaaat taagggtaat aagtacaaat acagaactaa tggtagaata taaatatatt
     3481 tatacataca tttataatac ttatcctata tgaataaaaa agtatattta gattataatg
     3541 ctacaacacc tgtagatcca agggttttgg aggtaatgat accatatatg aaggatatgt
     3601 atggaaatcc ttccagtgtg cattcattcg gtggagactc aaaggctgcg cttgatgatg
     3661 caagggaaca ggtggcagaa ctcataggtg caagacagag agatatctat tttactacag
     3721 gtggcagtga gagtaataat tttgctatta aaggtatagc ctatcagtta aaagataaag
     3781 gtaggcattt aatcacaact caggttgagc acgcatcagt tttagagacc tttaagtatc
     3841 tggaatctga aggatatcaa gttatctatc tatctataga tagggatgga atgcttgatg
     3901 tggatgaact acgagatgtg atttgtgaag atactattct tgtgtccatt a
//
LOCUS       DBNGSV010000224         6516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1078740, whole genome shotgun sequence.
ACCESSION   DBNGSV010000224 DBNGSV010000000
VERSION     DBNGSV010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1078740"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /gene="atpB"
                     /locus_tag="AC159U_08180"
     CDS             complement(<1..602)
                     /gene="atpB"
                     /locus_tag="AC159U_08180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3337075.1"
                     /translation="MGHFSWFTHLDLIKYDHLFGMVIVLVLLLLAALKVRSKIVNSDT
                     VLPGEKFSLTNIFEILSIDFLLNLIAGIFGSKKAARTYFPILATSFFVILFANILGLI
                     PGFLPPTGNFNTTLALALVIFVMYNYYGIKENGWSYLKHFLGPMIYLAPLMLIIEIIS
                     HLVRPLSLSLRLFWNMTGDHLVLGIFTDLTHIIIPVLFVGL"
     gene            complement(605..964)
                     /locus_tag="AC159U_08185"
     CDS             complement(605..964)
                     /locus_tag="AC159U_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337076.1"
                     /translation="MSESGLFEFSRIEKAALIVTLVIVSANALLGYQDMAMGAAAGGF
                     LFTANIIAIRFIVNLLIGQKQTKAFSVFAIVLKMLMLIALAVGLFIFTKINIYGFFIG
                     VTGVVIVLIGESLRGNK"
     gene            complement(957..1142)
                     /locus_tag="AC159U_08190"
                     /pseudo
     CDS             complement(957..1142)
                     /locus_tag="AC159U_08190"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
     gene            complement(1196..2479)
                     /gene="hemL"
                     /locus_tag="AC159U_08195"
     CDS             complement(1196..2479)
                     /gene="hemL"
                     /locus_tag="AC159U_08195"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR3337077.1"
                     /translation="MHKTKSENYFKRAKQLMPGGVNSPVRSFSAVGGAPLFIARAKGP
                     YLHDEDGNRYVDFVASWGPLILGHSHPNVIKALKTASALGTSYGAPCKAEIDLASIVT
                     GSFKSIDMVRMTNSGTEATMSAVRLARGYTGRDYIVKFEGCYHGHADYLLVKAGSGAT
                     TFGHPSSPGVPKSFTSKTLLAKYNDLDSVKKLFNKYGDQIAAIILEPVAGNMGVVTPE
                     KGFLKGLRSITNKYKSLLIFDEVITGFRLSKGGAQQIFGVYPDLTCLGKIIGGGLPVG
                     AYGGKKAIMKHIAPDGPVYQAGTLSGNPLAMAAGIATLKELANVSVYKKLEKTTVNFV
                     GEINEIIKKLGINANINQIGSMFTLFFNSGKVCDYETALCSDTKIFSRFFKNLLSAGI
                     MFPPSQFESVFISTAHTEKELNFALNSIHKALKDL"
     gene            complement(2636..3337)
                     /locus_tag="AC159U_08200"
     CDS             complement(2636..3337)
                     /locus_tag="AC159U_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3337078.1"
                     /translation="MRWDVRLLTIIPALLVGLMLAGCPTPPPTQELADAEAAYADATA
                     AGCPECAPEACAAAESALKKGRFLATDFCSELEARRLLIDAKAKALEAQNICATTPPP
                     DMTDHGLKDVFFDFDRSNIRADAEAVLAENAETLKNNPDLNIVIEGYADLRGSVQYNI
                     GLAQRRANSAKAYLEGLGIDSSRIQAVGVGETEIFGEGTSSDAYQLNRRAHFVEGNSP
                     GALIYFKKADEQPAS"
     gene            complement(3466..4200)
                     /gene="ybgF"
                     /locus_tag="AC159U_08205"
     CDS             complement(3466..4200)
                     /gene="ybgF"
                     /locus_tag="AC159U_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR3337079.1"
                     /translation="MKKSLLLILFPVALGAGCVATHDDMGNIYARQSRLEAKTKKLEQ
                     RVEEMQYQPGSESFSASEQKIAELEQRIAVLERSQERLTSAVQRTNRYSSADPVIPSD
                     SEKLIENGTTRDFATTEVDQEAVIFNRGYKFLSENKYEDARGQFSEFLQKYPQSPQSS
                     DAAFWIAESYFRQGLYEEAILDFQKFIEDYDKDIKVPLAYYKQGESLVSIDRKEEAII
                     FFQTLIDKFPQSEEARSAKEKLRTLQ"
     gene            complement(4213..4947)
                     /locus_tag="AC159U_08210"
     CDS             complement(4213..4947)
                     /locus_tag="AC159U_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3337080.1"
                     /translation="MKNLQTKLLILVAFLSFGLMITSCAQKLDKEFSDANTELEKLRE
                     QGGDPEDIAEIEAMIAEARELLASGDRLGAQKKLEEARFRAIELQGDLQLANRSDTRD
                     VPEGTPIEIGLVDILFDYDQSRVRPDAGPVLSQNARIIKENSARISKVVIEGYCDTRG
                     TDEYNLALADKRAKSVEAYLAGIGVDINIIESVGKGETDEWGADASESAYAKNRRAHF
                     KVLSSGGGSSDSMMMDSDDAIMEEEQ"
     gene            complement(5051..>6516)
                     /locus_tag="AC159U_08215"
     CDS             complement(5051..>6516)
                     /locus_tag="AC159U_08215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3337081.1"
                     /translation="RVAENITEWTQKDAEGRKLEEVLIWDSEANSNINDIQTGRDLQQ
                     GKIVGTKDEIKIRSKTDKQKIISASGSYIKDNNNNIVGNVIVFNDITEKQKLRQELVK
                     VQKLESLETLAGGIAHDFNNILTGIIGNLSLSKMYVDTDDKIHKRLTEAEIECFKAAD
                     LTKQLINFAEGVSPNKKISSISDLVKESVKFLLTGSKVKCTFECSENIWLVEVDQKQF
                     IQSIYDIVLNSLQAMPDGGTIKTSVQNVTIKQNVNNVTVDGEYVRIIIEDNGTGISED
                     HLAKIYDPYFTTKENHAGLGLSNTYTTIKSHKGFIKIDSSEDKGTKVSIYLPASRDVI
                     PLLDEEVAQTVIGKPDDNQQHGKILVMDDDEVIREIAGSILCYLGYEVSFASDGKEAI
                     DLYREAYEGNQKYDVVIMDLTVPGGMGAEDTIKQLQNIDPEVKAIVISGYSNAQIVSN
                     FYKYGFKGAVIKPYKIEELTRTVKDVLVDKNMNTLPI"
BASE COUNT         1879 a         1410 c         1064 g         2163 t
ORIGIN      
        1 agacctacaa aaagcacggg gataataata tgggtgaggt cagtaaaaat tccaagtaca
       61 aggtggtcac ctgtcatatt ccaaaaaagc ctcagtgaaa gtgaaagtgg tcttacaagg
      121 tgacttatta tttcaattat taacataagc ggtgccagat atatcattgg gcccaggaaa
      181 tgttttaaat agctccagcc gttctcttta attccgtaat agttgtacat aacaaagatt
      241 acaagcgcta gagcaagtgt agtattaaag tttccggttg gtggaagaaa ccccggaatc
      301 aggccgagaa tattagcaaa taaaataaca aaaaatgagg tggctaaaat tggaaagtat
      361 gtcctggcgg cttttttcga tccgaatatt cctgcaatca gattaagtag aaagtctata
      421 gacaaaattt caaatatatt tgttaaggaa aatttctcac caggcagtac cgtatcggag
      481 ttaactatct ttgaccttac ctttagagct gccagcaaaa gtaacactaa tactattacc
      541 attccaaata aatgatcata tttaattaaa tccaaatgag taaaccagct aaaatgtccc
      601 atatctattt attacctcgc aaactttctc ctattagtac tatcactacc ccagtaaccc
      661 caataaaaaa cccatatatg tttatctttg taaatataaa aagccctacg gcaagagcta
      721 tcagcataag cattttgagc acaatagcaa acacactaaa cgctttggtt tgcttctgac
      781 caattaacag gtttacaatg aatcttatag caataatgtt tgcagtaaat aaaaacccgc
      841 cggctgcagc tcccattgcc atatcctgat aacctagaag cgcattagca ctcacaatta
      901 caagggttac aattagcgca gctttttcaa tcctgctaaa ctcaaataat ccactttcac
      961 tcattactgt cgttgtcata atgttttaat aactttatta taatagttgt acctgcaaaa
     1021 aggccgatta aaagaccgag aaaagtaaac cagggagtat ctaatttaag ctttccgtct
     1081 atataatcac ctaataccaa gcctgctata actgaaaacg caagctctac tccaactact
     1141 aaatactttg cccaatttag gtttttattt ctaacttgct aaaaaccctc caaacttata
     1201 aatctttaag cgctttatgt atcgaattta gcgcaaaatt aagctctttt tccgtatgag
     1261 ctgtagaaat aaaaacagac tcaaactgcg aaggcgggaa cataatacca gcactcaaaa
     1321 gatttttaaa gaaccttgag aaaatttttg tatccgagca aagagcagtc tcataatcac
     1381 aaactttccc actgttaaaa aagagtgtaa acatagagcc tatctggtta atattggcat
     1441 ttatacccag tttttttatt atttcattta tttctcccac aaaattaact gtggtttttt
     1501 caagtttttt gtatacagat acattcgcaa gttcttttaa tgttgcaata ccggctgcca
     1561 ttgcaagtgg attacctgaa agggtacccg cctgatatac gggtccgtcg ggtgcaatat
     1621 gcttcattat agcctttttt cctccataag cccctacggg aagtcctccg cctattattt
     1681 taccaagaca cgtaaggtct ggataaaccc caaatatctg ctgtgctcct cctttactga
     1741 gcctgaatcc tgttattacc tcatcgaata taagaaggga tttgtattta tttgtaatag
     1801 aacgaagccc ctttaaaaaa cccttttctg gggttactac acccatatta cctgcaacag
     1861 gctccagtat tattgctgcg atttgatccc catatttatt aaacaacttt tttaccgaat
     1921 caagatcatt gtactttgca agaagtgttt ttgaagtaaa actctttggt actccggggc
     1981 tgcttggatg gccgaatgtg gttgctcctg atcctgcttt tacaagcaga taatcagcat
     2041 ggccgtgata acaaccttca aatttaacta tatagtccct cccagtgtat ccccttgcaa
     2101 gtcttacagc actcattgta gcctcggtac ccgagtttgt catacgaacc atatcgatgg
     2161 acttaaacga tccagttaca atactggcaa ggtctatctc ggccttgcat ggcgctccgt
     2221 agcttgtccc gagtgccgaa gcagttttta gagcttttat aacgtttggg tgtgaatggc
     2281 ccagaattaa aggcccccag gatgcaacaa aatctacata cctgtttcca tcttcatcat
     2341 ggagataagg cccttttgcc ctggctatga atagaggggc accgcctaca gcggaaaatg
     2401 acctcacagg gctgttcacc cctcctggca ttagttgttt tgctctttta aagtagtttt
     2461 ctgatttggt tttatgcata agattctata ttaattgcta atttaaaaaa aaagaataat
     2521 aaacagagta aatcactcta agtcaagtga attggaaaag agcaataaaa aagggaagaa
     2581 agcttaaaag ctcccttccc tttctataac ttcaaataaa cttaattgtt aaattttatg
     2641 aagctggctg ctcgtctgct tttttgaaat aaataagagc gcctggtgaa tttccttcta
     2701 cgaagtgtgc tcttctgtta agctgataag catctgatga agtaccttca ccaaatatct
     2761 ctgtttcacc aactcctaca gcctgaattc ttgagctgtc aataccaaga ccttcaagat
     2821 atgctttagc gctatttgct cttctttgag caaggcctat attgtactgt actgaacctc
     2881 taaggtcagc ataaccttca attacaatat taagatctgg attgtttttc aatgtttcag
     2941 cattctctgc aagtactgct tcagcatctg ctctaatatt tgatctatca aaatcaaaga
     3001 aaacatcttt taaaccatga tctgtcatat ctggaggtgg tgttgtagca cagatatttt
     3061 gagcttctaa agcttttgct ttagcatcaa taagaagcct tcttgcttca agctcgctac
     3121 aaaaatcagt agcaaggaat ctaccttttt taagtgcgct ttcagctgct gcacaagctt
     3181 caggtgcgca ttccggacag cccgctgctg ttgcgtctgc gtatgcggct tcagcatctg
     3241 caagttcctg tgttggtggt ggtgtaggac accctgccaa catcaaccca accagcaatg
     3301 ccggtataat tgttaataat ctaacatccc atctcatatt acattcctcc tttaactata
     3361 attatttttt tgctgtactg taatatatat aattattaac agatttttct attgtaatac
     3421 atttgtaata attacacaag cattttttgc aataatcaga aatatttact gtagcgttct
     3481 taatttctct tttgcgcttc tagcttcttc agactgagga aatttatcaa taagggtctg
     3541 aaaaaatata atcgcttcct cttttctgtc tatactcaca agagattcac cctgtttata
     3601 atacgctaaa ggaaccttaa tatccttatc atagtcttct ataaactttt gaaagtccag
     3661 aattgcttct tcatacaaac cctgcctgaa atatgactcg gcaatccaaa acgccgcatc
     3721 ggaggactgg ggagactgag gatatttctg taaaaactct gaaaactgcc ccctggcgtc
     3781 ttcatactta ttttcactaa gaaacttata acctctgtta aatataacgg cttcctgatc
     3841 aacctcggtt gttgcaaaat cccttgtagt gccattttct attagttttt cagagtctga
     3901 aggaatcaca ggatcggcgc tcgaatatct attggttctc tgaactgccg aagtgagtct
     3961 ttcctgagat cgttctaaaa cagcgattct ttgctcaagc tcggctatct tttgttcaga
     4021 ggctgaaaat gattcagaac ccggctgata ctgcatttct tcaaccctct gctcaagctt
     4081 ctttgtcttg gcttcaagtc ttgactgcct tgcataaata ttgcccatat catcgtgtgt
     4141 agccacacaa cctgctccta gcgctacagg gaaaagtata agtaatagac tctttttcat
     4201 tatcttaata aattattgtt cttcttccat aatagcgtca tcagaatcca tcatcataga
     4261 atcacttgaa ccgccaccgc ttgaaagcac tttaaagtgc gctcttctgt tctttgcata
     4321 agcagattca gaagcatcgg ctccccactc atctgtctca ccctttccta ctgattcgat
     4381 aatgtttatg tctactccga ttcctgcaag atatgcttca actgatttcg ctcttttatc
     4441 ggcaagcgct aagttatatt cgtctgtacc gcgagtatca caatagcctt cgattactac
     4501 cttactaatt cttgcactat tttcttttat gatccttgca ttttgactaa gtacagggcc
     4561 tgcatcgggt cttactcttg attggtcata atcgaagagt atatcaacca atcctatttc
     4621 aattggtgtt ccttcaggaa catctcgggt atcagatcta ttagctaact gaagatcacc
     4681 ctgtaactca attgctctga atcttgcttc ttcgagtttt ttttgtgcac cgagacgatc
     4741 ccctgaagcc agcagttctc ttgcctcggc aatcattgct tctatttctg cgatatcctc
     4801 aggatcaccg ccttgttctc ttagtttttc taactcagtg ttggcatcac taaactcttt
     4861 atcaagcttt tgcgcacaac ttgtaatcat taaaccaaaa gataaaaaag ctactaaaat
     4921 aagtaatttt gtctgtaaat ttttcataca agatctccct ataataagat attttcaaag
     4981 tctgacttat tttacatttc aagtcaaggt gattaatagc taatcaataa attgtaataa
     5041 tttaccagtg ctatattgga agagtattca tattcttatc cacaagaaca tctttaacgg
     5101 ttcttgtaag ttcttcaatc ttgtatggtt tgatcacagc acccttaaag ccatatttat
     5161 agaaattaga tactatttgt gcgtttgaat acccgcttat tactatcgct ttaacctcgg
     5221 gatctatatt ttgtagctgt tttattgtat cttcagcacc cattcctccc ggtaccgtaa
     5281 ggtccataat aacaacatca tatttctggt tgccctcata tgcctctcta tatagatcaa
     5341 ttgcctcttt gccatctgag gcaaatgaaa cttcatagcc aaggtagcat agaattgagc
     5401 ctgcaatctc tcttataacc tcatcatcat ccattaccag aattttaccg tgctgctgat
     5461 tatcatcagg tttaccaata accgtctggg caacttcttc atcaagaagg ggtataacat
     5521 ccctgctcgc cggcagatat atagatactt ttgttccctt atcctcagaa gaatcgattt
     5581 ttataaaccc tttgtggctc ttaattgtcg tgtaggtatt ggaaaggcca agacctgcgt
     5641 ggttttcttt tgtcgtaaaa tacggatcat atatctttgc aagatgatct tcagaaatcc
     5701 cagttccgtt gtcttcaata ataattctta catactcgcc gtccactgtc acattgttca
     5761 cattttgctt tatggttaca ttttgaactg acgttttgat ggttccccca tcgggcattg
     5821 cctgaagaga gtttagaact atatcataaa tagattggat gaactgcttt tgatcaacct
     5881 caactagcca tatattttca gaacattcaa atgtacattt tacttttgaa cccgttagca
     5941 agaacttaac agactcttta acaagatcgg atatagatga tatttttttg ttaggtgaaa
     6001 caccctctgc aaaatttatt agctgctttg taagatcagc tgccttaaaa cattcaatct
     6061 ctgcctctgt aagcctttta tgtattttgt cgtctgtatc tacatacatc tttgatagcg
     6121 aaaggtttcc tattattccc gtgagaatgt tattaaaatc atgagctata ccgcctgcaa
     6181 gggtttcaag cgattcaagc ttttgcacct ttacaagctc ctgccttaac ttctgctttt
     6241 ctgtaatatc attaaataca attacgtttc ctacaatatt attgttgtta tcctttatat
     6301 atgagccgct tgcgcttatt atcttttgtt tatctgtttt ggatctaatc tttatttcgt
     6361 cttttgttcc aactattttg ccctgctgca gatcacgtcc tgtttgaata tcgttgatat
     6421 tactgtttgc ctcagagtcc catatcaata cttcttctag ttttctaccc tcggcatctt
     6481 tttgagtcca ttctgtgatg ttctctgcca cacgat
//
LOCUS       DBNGSV010000225         4577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1702543, whole genome shotgun sequence.
ACCESSION   DBNGSV010000225 DBNGSV010000000
VERSION     DBNGSV010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1702543"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            706..1056
                     /locus_tag="AC159U_08220"
     CDS             706..1056
                     /locus_tag="AC159U_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337082.1"
                     /translation="MHTKKLFEIYEDSLKNTSGLEGLVSEADLEQAITKKKYDLQDLA
                     LIEVVMNDDEKQFHESLKENYEALSADLTKKNGPATETDPLKEQLLEIFISNLNYYID
                     LFYNSLLNRHFAGG"
     gene            complement(1068..2360)
                     /locus_tag="AC159U_08225"
     CDS             complement(1068..2360)
                     /locus_tag="AC159U_08225"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR3337083.1"
                     /translation="MAKKDTLTITDNRTGKEYEVPITNDTIKAMDLRQIKVKESDFGM
                     MSYDPAYSNTAHCTSRVAFIDGDKGVLVYRGYPIEVLAEKSNYLEVAYLLLNGQLPNK
                     KEYKEWEHNITHHTYVHENIKKFIGGFRYDAHPMGMLLSTVGALSTFYPDAKDIFNEE
                     NREIQIQRLISKMPTLAAFSYRHAMGLPFVYPDNSLSYPGNFLNMMFNIAQKEYKPNK
                     AIERAIDILFILHADHEQNCSAAAMRNVGSSLPDPYSATAAAIAALYGPLHGGANEGV
                     LQMLAEIGSKDQIPKYVERAKKGEFRLMGFGHRVYKSYDPRAKIIKKIAYDVFEETGE
                     NPLLEMALELEKIALSDEYFVKRKLYPNVDFYSGLIYQSMGLPVSMFTVLFAIARTSG
                     WLAQWKEFLTDPESRIARPRQVYLGDMGKKYVPLAKRK"
     gene            complement(2552..>4577)
                     /gene="ptsP"
                     /locus_tag="AC159U_08230"
     CDS             complement(2552..>4577)
                     /gene="ptsP"
                     /locus_tag="AC159U_08230"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3337084.1"
                     /translation="SKRPLIAMPASEHPYYKYFPEIGEEEYESYIGLPIQLQNKFMGV
                     LVGQTNERRLIHPSEESLFQIIASRLAALLEVADNLERLKPASSKKKKVRMTLQGKGV
                     SSGFAVGNVYIFKGMYDQYRPDDVNKGTAEQESQKIIDAFINVENDLNFLIKDLKNQH
                     FLSDSEIGIFETHLMILGDSGFKNKILDRITKKKASAERALIDTIEEVARHFESLTDI
                     YFKERAGDFRDIGQRVLHYLVQSRTNGDVSVEVKKGSILVAKDIGPSFVSMLYKNQIS
                     AIVTEKGGETSHLVILAKSLGIPAVVGIENVSDILSAGEKIIVDGKTGFVITQPGKEL
                     INQYQEKFSKYERLRDYIEKESLKTPNPDIQLKLTANIGFPVDVSLANQYNLSDVGLF
                     RTEFAFSQFEKWPELKEQVDVYDRVAENFSGYITVRTLDVGADKLLPYLKFPEEENPL
                     LGLRAIRFSMEYLETFQDQIKAILLAGTKGHKFRILLPMVTNIWEVETAKELIEQIGD
                     ELGVNKTELPPLGMMIEVPGLIYQLEDYRHLVDFISIGTNDLIQYLLAVDRNSNEVGH
                     LYSSYHPSIIRMLYDLFRKTEHLHMEISLCGEIAGSLAGSLILLSLGYRVLSISPTRA
                     PVIRYLVNNIDRDLLEEIKKHILFEKKEKEIKTYLNEVIEKIYPSLSELY"
BASE COUNT         1340 a          935 c          795 g         1507 t
ORIGIN      
        1 cggcacttcc aatacaaaca tttgtattag cttgcttatc ttactatttg atagttttgt
       61 attcttaagt aacttctttt gcatagtttc agcttagtat actttgctgt cctatgcgag
      121 ctcctaattt ttaatatcat accatcagta ttaattctct agacctgaga aattaatagt
      181 ttgataggaa ctctattaac caaatctaat agatttaata aaataataat aattattatt
      241 aagtaatata tttcattcac cttctatcaa actatcaaag cctcactgtt aggatgaaca
      301 aattcatagt ttgacaataa ctcttagaaa tttacttagt ctcttaatct aataatttaa
      361 tggttaagaa aaaaaattag agactaagag actttcttat aaaaaatcaa tattatcagg
      421 aagttataat cacttaggct caaaagctca gggttagggt aaaacatttg agccttgagc
      481 ctagtatata aaaagatcaa aagatcacgt atcaggccat agggtatgaa ccttgatctt
      541 ttgattctaa ctatgattat tttaaataat gccaaattgc catgttttca gggtttatgg
      601 taacaaactg gcatttttgc aataacagtg ttttatatac actgcaatga catatatttc
      661 ccttccctct ttactaactc ccatacatta attaacattg tatctatgca tacaaagaaa
      721 ctgtttgaaa tttatgagga ttcgctgaaa aacacttccg ggcttgaggg tttagtttcg
      781 gaggctgatt tagagcaggc tattacaaag aaaaaatatg atttacagga tttagcatta
      841 attgaagtgg ttatgaatga cgatgaaaag cagtttcatg aatcacttaa ggaaaattac
      901 gaggcgctct ctgccgatct tacaaaaaaa aacgggcctg ccactgagac agacccgctt
      961 aaagaacaac ttttagaaat ctttatatct aatctgaatt actatataga ccttttttac
     1021 aactccctgc taaacaggca ctttgcaggc ggctaaagac tatatactta ctttctcttt
     1081 gcaagaggaa catacttctt gcccatatcc ccaagataaa cctgacgagg tcttgcaatt
     1141 ctgctctctg ggtcagttaa gaactctttc cactgtgcca gccatccgct tgttcttgcg
     1201 attgcaaaaa gtacggtgaa catgctcacc ggaagaccca tgctctgata aataagccct
     1261 gagtagaagt ctacattagg atagagcttt ctcttaacga agtattcgtc agaaagagcg
     1321 atcttttcaa gctcaagcgc catttcgagt agtggatttt ctcctgtttc ttcaaacaca
     1381 tcatatgcga ttttctttat tatctttgcc cttggatcgt aggatttata aaccctgtgt
     1441 ccaaaaccca tgagtctgaa ttcacctttt ttggctcttt caacatattt tggaatctga
     1501 tcctttgagc cgatctctgc taacatctga agtacgcctt cgttagctcc accatgcaga
     1561 ggtccataaa gagctgcaat tgcagcagca gttgcagaat aaggatcagg aagcgagctt
     1621 cctacgtttc tcattgcagc agcgctgcag ttctgctcgt gatcggcatg tagaatgaat
     1681 agtatatcga ttgctctttc aattgccttg tttggcttat attctttttg agcgatgtta
     1741 aacatcatgt ttaagaaatt accagggtag cttaagctgt tatcaggata aacaaaaggc
     1801 agacccattg cgtgacgata agaaaatgca gcaagtgtag gcatcttgga aattagtctt
     1861 tgaatctgaa tttctctgtt ttcttcattg aaaatatctt tagcatctgg ataaaaagta
     1921 gaaagtgcgc ctactgtgct aagaagcatt cccatggggt gtgcatcata tctgaaaccg
     1981 cctatgaatt ttttaatatt ttcgtgaaca taagtatggt gagtaatatt gtgttcccac
     2041 tctttgtact cttttttatt aggcagctgg ccatttaaca gcaggtaggc aacctctagg
     2101 tagttacttt tttcagccag tacttcaatt ggatatcccc tgtataccag tactccctta
     2161 tcaccatcaa taaaagctac cctgcttgtg cagtgagctg tattgctgta tgcaggatcg
     2221 taactcatca tcccgaaatc gctttccttg accttaattt gtcttaaatc catagcctta
     2281 atggtgtcat tagtaatagg aacttcgtat tccttaccag tcctgttatc agtgattgtt
     2341 aatgtatctt tctttgccac aatcagacct ccttcgtgtg atagtattca atttgtaaga
     2401 ataaaaagtt aaatattcta gcaaataacc taaccacagt atcaaaacac gcttaatttg
     2461 tcaaatggaa ttaacactga attaaaatta tgtatatatt ataaaaagta ataaaaacag
     2521 agttaataga gatagaaata tagaatttta gctagtaaag ctcactgagt gaaggataaa
     2581 ttttttctat aacttcattt aaataagtct taatctcttt ttcctttttt tcaaaaagaa
     2641 tatgcttttt tatttcttcc agtaaatccc tgtctatatt gtttacaagg tatctaataa
     2701 caggagccct tgtgggtgat atgcttagga cccggtagcc aagagataga agaattagtg
     2761 atccggcgag tgatccggct atctcaccac aaagcgatat ctccatatgc aggtgctctg
     2821 tttttctgaa aagatcatag agcatcctta ttattgacgg atgatagctt gaatacagat
     2881 ggcccacttc gtttgagtta cggtctacag caagcagata ctggataagg tcatttgtac
     2941 cgattgatat aaaatcgaca agatgtctgt agtcttcaag ctgatatatg aggcccggaa
     3001 cctctatcat cattccaagc gggggaagct ctgttttgtt taccccaagc tcatcaccaa
     3061 tctgttcgat taattccttt gcagtctcga cttcccatat gtttgttacc atgggcagaa
     3121 gaatcctgaa tttatgcccc ttcgtgccgg caaggagtat tgcctttatc tgatcctgga
     3181 aagtctcaag atactccatc gaaaacctga ttgccctaag cccaagcaga gggttctctt
     3241 cttctggaaa tttaagataa ggaagcagtt tatcggcccc cacatccagg gttctgactg
     3301 tgatatatcc gctgaaattt tcggccactc tgtcgtacac atctacctgt tcctttaact
     3361 cgggccactt ttcaaactga gaaaatgcaa actctgtcct gaaaagtcct acatcggaaa
     3421 ggttatactg atttgctaaa gatacatcca cgggaaatcc aatgtttgca gttagtttta
     3481 gctgtatgtc agggttaggc gttttgaggc tttccttttc tatataatct ctcaaccgtt
     3541 catatttgga aaatttctcc tgatactgat ttatgagctc ctttccgggc tgagtgatta
     3601 caaacccggt tttaccgtct acaattattt tctcaccggc gctaaggata tcagatacat
     3661 tttcaatacc cactacagcc gggataccca gtgacttagc cagaattaca aggtgagagg
     3721 tctcgccgcc tttttcagtt actatggcag atatctgatt tttatataac atggaaacaa
     3781 acgaggggcc tatgtccttt gctacaagga tcgatccctt tttaacctct acgctaacat
     3841 ctccatttgt ccttgattgg actagataat gaagcaccct ctgtcctatg tccctaaaat
     3901 ccccggccct ttccttaaaa taaatatctg taagactctc aaaatgcctc gcaacttctt
     3961 caattgtatc aattagagct ctttctgccg atgctttttt cttcgtaatt ctatcaagaa
     4021 tcttgttttt aaatcccgaa tcaccaagaa tcataaggtg agtttcaaaa atacctatct
     4081 cactatcaga caggaagtgc tggtttttaa ggtcttttat taaaaagttc aggtcatttt
     4141 caacattaat aaatgcatca ataatctttt gcgactcttg ttcggcagtt cctttattaa
     4201 catcatcggg tctgtactga tcatacattc ccttgaatat atatacattt cctacagcaa
     4261 accccgaaga cactccctta ccctgcaatg tcatcctaac tttctttttc tttgaagacg
     4321 ccggttttaa tctctcaaga ttgtcagcta cttccaacag tgccgcaagt ctgctggcta
     4381 ttatctgaaa aagagattct tctgaaggat gaataagacg ccgctcattt gtctgcccaa
     4441 ccagtactcc cataaattta ttttgaagct ggattggaag tccaatgtag ctttcatatt
     4501 cctcttctcc tatttcggga aagtatttat agtatgggtg ctctgatgcc ggcattgcta
     4561 tcagtggtct tttggat
//
LOCUS       DBNGSV010000226         2026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1010680, whole genome shotgun sequence.
ACCESSION   DBNGSV010000226 DBNGSV010000000
VERSION     DBNGSV010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1010680"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1649
                     /locus_tag="AC159U_08235"
     CDS             join(481..742,742..1649)
                     /locus_tag="AC159U_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611339.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3337085.1"
                     /translation="MAKKKYTPEQIIQKLREAEVRLSKGESVGKMCRSLGVSEQTYYR
                     WRNEYGGMRIDQAKRLKEIEKENTRLRKLVADLSLDNAILKEVKQGKLLSPVRKRRAV
                     SHACSILKISERRACRVLGQARSTQRCVRRIPDDEERLVARITALASQYGRYGYRRIT
                     GMLINEGWKVNHKRVERLWRREGLKIPKKQPKRRRLWLNDGSCIRLRPTHRNHVWAYD
                     FMSCRTYDGRPLKILTVVDEYTRECLALVVERRLRSDDVLHCLTDLFVSHGIPEHIRS
                     DNGPEFTAQAVRRWLGKIGVKTMYIEPGSPWENGYNESFNGKLRDELLNGEIFYTLKE
                     AQVLIENWRREYNTIRPHSSLGYKPPAPETIQPDSCTPLIPLLNLPQGECTRPLN"
     gene            1724..>2026
                     /locus_tag="AC159U_08240"
     CDS             1724..>2026
                     /locus_tag="AC159U_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR3337086.1"
                     /translation="MLNQSCKNKSRIGSTVRVSHTSGHEPIAIIGIGCRFPGNANDPE
                     SFWKLLCRGVDAITEIPADRWNVYKFYDPNPEKPGKSYARYGGFIKGVELFDNEFFG"
BASE COUNT          600 a          386 c          517 g          523 t
ORIGIN      
        1 acttcaaccc tcgttgtaaa gtgaaggaag taggattaga taaccacctt gagatagaag
       61 gagagtatga ttattcctgt aagtacttag tggttatcag ggggctggga tactgtagat
      121 gtcctctttg tgtttatctc agcaaggcgg atttcaaatg acatttagct ctttgcactg
      181 tatattgtgc gtggctattt cagaaaaaat gagtaacatt ctaccgagat tgtcgaaaaa
      241 cccttttgta aagagcgttt cttagtctgt tgtatcagat tttatgaaat gtatgtgagg
      301 ttcaactttt tttgtgggaa tacctactta tcctactgtt tggtctctac ccttctgcta
      361 acgcagtccc aaagtttgct atcttttcta tattgtgtac catactgacc tgccccccaa
      421 aaaccgatcc acatgttatg gtagtatgtg gtaggataaa acaatgaaag ggggcatact
      481 atggcaaaga agaagtatac accggaacag attattcaga aactacgaga agcagaggta
      541 cgactgagta aaggagaatc cgtaggaaag atgtgtcgaa gtctcggggt tagtgagcag
      601 acctactacc gttggcgtaa tgaatacggc ggtatgcgaa ttgatcaagc aaagaggctc
      661 aaagagatcg agaaagagaa taccaggtta agaaagctag tagcggatct gtcacttgac
      721 aatgctattt tgaaggaagt aaacaaggga aacttctaag cccggtaaga aaacgccggg
      781 cggtcagtca tgcatgcagt attctgaaga tttctgagcg gagagcctgt cgagtactgg
      841 gccaggcgag atcaacccaa cggtgtgtaa gaaggatacc ggatgacgag gagcggctgg
      901 tagcccggat tactgctctg gcaagccagt atggcagata tggctatcgc cggattacgg
      961 ggatgctcat aaatgaggga tggaaagtga atcacaagcg tgtagagcga ttatggagac
     1021 gtgagggact taagatacca aagaagcagc caaaaagaag aagactgtgg ctcaatgacg
     1081 gatcatgtat tcggctccga ccgacccatc gaaaccacgt ctgggcatat gattttatga
     1141 gttgccgtac gtatgatgga cgacccctta agatactgac agtagtggat gaatatacac
     1201 gggagtgcct tgcactggtc gttgagcggc gattacgatc agatgatgtg ctgcactgtt
     1261 taacagacct ttttgtgagt catggtatac cggaacatat ccgttccgac aatggtcctg
     1321 agtttaccgc acaagctgtc cgccgatggt tagggaagat cggagtaaaa accatgtaca
     1381 ttgaaccggg aagtccctgg gagaatggat ataatgagag tttcaatggt aagttacgcg
     1441 acgaactctt aaatggagag attttctaca cgctcaagga ggctcaggtg ctgatagaaa
     1501 actggcgtcg tgaatacaac accatcagac cgcacagttc cttggggtac aaaccaccag
     1561 caccggaaac cattcagcca gattcctgta cgccattaat accactcctg aatttacctc
     1621 agggagaatg tactagacct ttaaactgaa gagtggtacc actattgggg ggagctcaaa
     1681 ttactataaa tgatttttat taatatgacc tggagatttc ctgatgttaa atcaatcttg
     1741 taaaaacaaa tcgagaattg gctcaacagt cagggtatcg catacatcag gacatgagcc
     1801 gattgccata attggtatcg gttgccgttt cccggggaat gcgaatgatc ccgaaagctt
     1861 ttggaaactt ttatgcaggg gtgtcgatgc cattactgaa attcccgccg atcggtggaa
     1921 tgtatataaa ttttacgatc ccaatccgga gaaaccgggc aaatcatatg cccgttatgg
     1981 aggatttatt aagggggtag agctgtttga taatgagttt tttgga
//
LOCUS       DBNGSV010000227         8640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1425412, whole genome shotgun sequence.
ACCESSION   DBNGSV010000227 DBNGSV010000000
VERSION     DBNGSV010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1425412"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="AC159U_08245"
     CDS             complement(<1..1549)
                     /locus_tag="AC159U_08245"
                     /inference="COORDINATES: protein motif:HMM:NF015228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3337087.1"
                     /translation="MSTNKSKSLKLHDDSISYDRIKSKHVVENLYSDFIPWNELDDFY
                     PYIHAPSGKRIEFQDDPAMIIGTAIFKSTGERVAIVAQQTPSDDSERIKLNFGLVKAD
                     GYGLSLNMMEYAERHNIMLHTYVDTIGGDPYEYSAEKLQSWLISYCQAKMISLKTKSI
                     SVVLGSGGSGGAIAIQLAHKRFMLSRAEYAVITSKGCSAILFRTADNEEEALKVLQPT
                     ADYMQKYGIIDDIIKEPMLDKADYFPKVLENINKALTKATNELKKSDVNYLREILIKN
                     IEQCGQIEQHHQKYNTFARKLKKLVRSSFSKSKNPDVSQMQIALYGGEPYVCNDEKDQ
                     EGKVVRAGCKNQTTEDEFRLNYQSCPKCSKQMPLTSDEYIEMLLDDGSFNEIRPDISL
                     DNIDSRFNFYDYSSSRQKMGKKTDSKDSLVVGYGMIFETPVAVAVSDFRFMGGSMGSV
                     FGEKMRLITEYALMNKLPLISISATGGARMQEGTVALYQMAKTIASSLKLKEAGLPYI
                     TILGHPTT"
     gene            complement(1627..2559)
                     /locus_tag="AC159U_08250"
     CDS             complement(1627..2559)
                     /locus_tag="AC159U_08250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3337088.1"
                     /translation="MKYRELGSTGLKVSEIGFGAWAIGGAGYGPTDDTESKKALHRAL
                     DLGVNFIDTADSYGNGHSEMLIGDVLESRGDRNAIICTKFGWDFYSESGIKSNLDEKY
                     IRFAVKQSLQRLKRENVDVYLIHSSDPQKIKEFDVINTLNSLKNEGIIRFFGISINDY
                     YYDKILPCLEQFNPDVVEVRLNLLDRIDKSGLFDLCKRTRTGMIIREPLANGLLSGKY
                     TKNSVFSKNDHRHGFSEGYLEQQLNNVELLKGYFGSNGNMICGSIKYTLRYESVSVVI
                     PGAKTTRQVEENICALDHDFDFDKYEKFKKENLI"
     gene            complement(2654..2980)
                     /locus_tag="AC159U_08255"
     CDS             complement(2654..2980)
                     /locus_tag="AC159U_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3337089.1"
                     /translation="MFEVTTKAANEIKRLISEDDEIDNAFLRVRVVPGGCSGFSYEMG
                     FDDETEENDQIFEVGDVKVAIDEFSYPYIKGGTLDFKDGLNGTGFHIENPNAVGQCGC
                     GTSFTV"
     gene            complement(3007..3489)
                     /locus_tag="AC159U_08260"
     CDS             complement(3007..3489)
                     /locus_tag="AC159U_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3337090.1"
                     /translation="MMTFIKFLFLVSVSFWLGSIFFFSAVTAPSIFGALPKPEAGVLI
                     TIIFNKYYLIQYVLAGISIISLLLLIKIDKDRNIFRYIRLALVCLMLIFTVFSGTYIR
                     NSALQAKSVMKHSDPGAEVYIKSEKTFKSSHRNSVIINGLVFLAGIVILLNIAKRNEL
                     "
     gene            3610..4605
                     /locus_tag="AC159U_08265"
     CDS             3610..4605
                     /locus_tag="AC159U_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3337091.1"
                     /translation="MKSNKYLILILTIFSLTFFAVCSSNSNSEMSDKELIDRLQNYFK
                     KSYAADLPHDAVLVVTGFENAQIADMRSGNIAITFSGRTVDIPFMLDKSGKYAVLGNP
                     IDTATFEETPIKGIRKGSITIARNTYPMLLTQDSKYLIAGEVIDTTVDKTKETMDKIS
                     LDDVPIKGNKNAKVTIVEYSDFQCPFCNRANPIIENVLKDYKDKIRVVYKQYPLPNHN
                     WATPASIASICSYKTAGHEAFWKYHDKIFENQRTITLENSAQKFAEFAKELKIDEKKF
                     GECMSDPKIAERVNNEMSEGQSIGINSTPSFVVNGIMVRGANEQGLKSAIESSLN"
     gene            4787..5239
                     /locus_tag="AC159U_08270"
     CDS             4787..5239
                     /locus_tag="AC159U_08270"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR3337092.1"
                     /translation="MKINTPKTIIGRKDRVDFPKLKLYNIDAKVDTGAYTTSIHCSTI
                     EPVKENNKKYVRFKLLDSTHPKYQKKTFKLPVYKEKTVKNSFGESELRFIVKSIIRLF
                     DKDYEVELSLTNREKMSCPVLIGRKLLKHDFIVDINRYNLSYKGKIKK"
     gene            5245..6438
                     /locus_tag="AC159U_08275"
     CDS             5245..6438
                     /locus_tag="AC159U_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HIR3337093.1"
                     /translation="MKIVIMSRGPRLYSTRRLLEACRERGHKAGVFDTTKFSINVESG
                     DPNLLYKNKYLPKYDAVIPRIGASLTFFGTAVLRQFEEMGLYCLNSSLGILSSRDKLR
                     SIQLLAKHNLGFPHTIFVKNKEMIIPAIEELGGAPVIIKLLEGTQGIGVILADTNKVA
                     EAIIETLQSTKQNVLIQKFVKESKGVDIRAIVAGNKVIAAMRRKAKGDEFRSNVHRGG
                     TVEKVELDDKYQQTAIRAAQIMGLSVAGVDMLEGNDGPQIMEVNSSPGFEGIERATNI
                     DVAGAIVEHLEQQALFPELDLRQRFTLDKGYGVVEIQIAKNSDIIGTAVKDAGFREKD
                     IVIMSIKRSNKMISNPKSTREILLGDTLLCFGKLENMKSLILENRIKRRKPRKKKVVN
                     NEVQI"
     gene            6438..7400
                     /locus_tag="AC159U_08280"
     CDS             6438..7400
                     /locus_tag="AC159U_08280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR3337094.1"
                     /translation="MPHIFSINGKKVALGENARIDLKIAKLPTHTVIDLPVYIRRSQT
                     DGPVLLITAGLHGDELNGVEIIRRFILSEDSIPTTGTLVILPIVNVYGFLNNSRTMPD
                     GRDLNRSFPGTKEGSLAGRIAYTLMNVVLPHVDYGVDFHTGAVNKNYPQIRCTFDIPK
                     NLELANAFSPAFIINSKLRDGSFRRAAQKEGKSILVFEGGESLRFDEVTIKEGIDGIK
                     RLMSYLGMNNSVFISNRETQVIKQSSWTRATHSGLYRPLVDYGDVVKKKQVIATITDP
                     YGETEYKLKSRLNGYVLAVRTLPIVNRGDAIMHIGTTNLDKEHN"
     gene            7403..8422
                     /locus_tag="AC159U_08285"
     CDS             7403..8422
                     /locus_tag="AC159U_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR3337095.1"
                     /translation="MYLIAVITGISLIGSFLCSLMEAALYSMPRSRIESLKRDGDPGG
                     IRLSKLRDKIDEPIAAILTLNTAVNVLGASWAGALVSNYYGDAWLGLFSAGFTAGVLF
                     FSEIIPKSLGVTYANVLAPKLSSFIQLLVTVLMPFVKASGYITRLWGKNSHLNMPTEE
                     DIISLTRLSNHGGEILKQEAKWVSNALKLDDVYVEDIMTPKNVVCTLEENTTLDQIDL
                     DSDHWRYSRIPVFSKEDENKIVGIVRRQDIFQNLLRGEKDQRIHNLMIAPDFVPTNML
                     LHDLLDRFLITRRHLFCVENKNEQYVGVVALEDVLEFLIGDEIVDELDLHEDMQKYSK
                     TQKED"
BASE COUNT         2853 a         1620 c         1583 g         2584 t
ORIGIN      
        1 ctgttgtagg atggccaaga attgtaatgt acggaagacc tgcttctttt aacttaagag
       61 atgaagcgat agtttttgcc atctgataaa gagccacagt accttcctgc atcctagctc
      121 ctcctgttgc agatattgag ataagaggaa gtttgttcat aagagcatat tcggttatca
      181 atctcatttt ctcaccaaat accgagccca tcgatccacc cataaacctg aagtcactaa
      241 cagcaactgc cacgggggtc tcaaatatca taccgtaacc aactacaaga gaatcctttg
      301 agtcggtttt cttacccatt ttctgcctgc ttgaagaata atcataaaaa ttgaacctgc
      361 tatcaatatt atccaaagaa atatcgggtc taatctcatt gaatgagccg tcatctagaa
      421 gcatttcaat atactcatcc gaagtaagcg gcatctgctt tgagcatttt gggcatgact
      481 gataattgag tctaaattca tcctcagtag tctgattttt acacccagcc cgaacgactt
      541 taccctcctg atccttttca tcattacaca catagggttc tccgccatat agagctatct
      601 gcatctgtga tacatcggga ttttttgatt tactgaaaga agatctgacc agctttttaa
      661 gtttccttgc aaatgtatta tatttttggt ggtgctgctc aatctgaccg cattgttcta
      721 tgtttttaat aagaatttca cgcaagtagt ttacatcaga ttttttgagt tcatttgtag
      781 ctttagttag ggctttattt atgttttcga gtactttggg gaaatagtct gccttatcga
      841 gcataggctc ttttataata tcatctatga ttccatattt ctgcatatag tcggctgtgg
      901 gctgcagcac cttgagggcc tcttcttcat tatcggcagt tctgaacaat attgcagaac
      961 aacccttgga agttataaca gcatactcgg ctcttgagag cataaaacgc ttgtgcgcaa
     1021 gctgaattgc tattgctccg ccgcttcctc cactgccgag aacaaccgaa atagatttgg
     1081 ttttaaggct tatcatttta gcctgacagt aagaaattag ccacgactgc agtttttcag
     1141 ccgaatattc ataagggtcg ccaccaatag tgtccacata tgtatggagc ataatgttgt
     1201 gcctttctgc atattccatc atgttaagag aaagcccgta accgtctgct tttaccagtc
     1261 caaaattaag tttaatcctc tctgaatcat ccgaaggagt ctgctgagct acaatagcaa
     1321 ctctctctcc cgttgactta aatattgcag taccgattat cattgcggga tcgtcctgaa
     1381 attctattct ttttcctgac ggtgcatgaa tatacggata gaaatcatca agttcattcc
     1441 aaggaataaa atctgaatac aggttttcta ccacatgttt ggattttatc ctgtcataag
     1501 aaatagaatc atcatgcagc ttaagacttt tcgatttatt agtgctcatt aaaatacccc
     1561 agataaaatt agttcggtct ctaagacctt agattatata tataaataat tataattaaa
     1621 gatatattag attaaatttt cttttttaaa tttctcatac ttatcaaaat caaaatcatg
     1681 atccaaagca caaatgttct cttctacctg ccttgtagtc tttgcgcccg gtattactac
     1741 agatacagac tcataccgta gagtatactt tattgatcca cagatcatgt ttccatttga
     1801 tccgaaatac ccctttagta attctacatt atttaactgc tgttctaaat acccttcact
     1861 aaaaccatgt ctgtgatcat tttttgaaaa tacagaattc tttgtatatt taccactcag
     1921 cagtccattt gccagaggtt cgcgtattat catccccgtt cgggttcttt tacataaatc
     1981 aaataacccg gatttatcga tcctatcaag caaattaagt cttacctcta ctacatctgg
     2041 attaaattgc tctaaacaag gcagtatttt atcataatag tagtcattta tggatattcc
     2101 aaaaaatctg attattcctt cattttttaa ggaattaagg gtattaatga catcaaattc
     2161 cttaattttt tgaggatcgg agctatgtat gagataaaca tcgacatttt ctctttttaa
     2221 gcgctgcagg ctctgcttca ccgcaaatct aatatatttt tcatcgagat tgcttttaat
     2281 acctgattct gaataaaaat cccacccgaa cttcgtacaa ataatggcgt tcctatcccc
     2341 tctggactcc aaaacatctc ctataagcat ttcactatgg ccgtttccat aagaatctgc
     2401 agtatcaata aaattcacac ctaaatccag tgccctgtgc aaggcttttt tagactcagt
     2461 atcgtcagtc ggcccatacc cagcacctcc aatcgcccac gcgccaaacc caatctctga
     2521 tacttttagt ccggtacttc caagctctct gtatttcata ttgattgtaa aattagtatt
     2581 aaaaacaaaa aaaccatgga tgagcagtca attgctcaac catggttata aattttgtag
     2641 ttttatttgt aaattaaact gtaaaagaag taccgcagcc acactgccct actgcgtttg
     2701 gattttcaat gtgaaacccc gtaccattga gcccatcttt aaaatccaga gtaccacctt
     2761 taatataagg ataactaaac tcatctattg caacctttac gtcgccaacc tcaaagatct
     2821 gatcattttc ttcagtttcg tcgtcaaaac ccatctcata agaaaatcct gaacaacccc
     2881 cgggtacaac tcttaccctg agaaatgcat tatcaatttc atcatcttct gatatcagtc
     2941 ttttaatctc attagccgct tttgttgtaa cttcaaacat acttactacc tcctgctaaa
     3001 tctgctctat aattcattac gtttagcaat atttaataaa ataacaattc ccgctaaaaa
     3061 tacaagacca ttgattatta cagagtttct atgagagctt ttaaaagttt tttctgattt
     3121 tatatatact tctgctccgg gatcagagtg tttcattaca gattttgcct gcagtgcaga
     3181 atttctaata tatgtgcccg aaaaaactgt aaaaattaac attaaacaaa caagtgcaag
     3241 ccttatatac ctaaatatat tcctgtcttt atcaattttt atcaataata atagcgaaat
     3301 tatagatata cctgcaagta catactgtat gagataatat ttattaaaaa taatagtgat
     3361 aagaactccg gcctcgggtt ttggcagtgc gccaaatatc gatggagcag taacagcgga
     3421 aaaaaagaag atgcttccaa gccagaagct tactgaaaca agaaataaga acttaataaa
     3481 tgtcatcata ataacagaga atttacatga ttttttggtt tttacatttt tagatggaaa
     3541 gtaacatagt tattgctatc ctattatgaa atgatatagt ttctaaattt tttttgggag
     3601 gaacagctaa tgaaatccaa taaatatctg attttaatcc tgactatatt ttctctgact
     3661 ttttttgccg tatgttcatc aaattctaat tcagaaatgt cagataaaga gcttatagat
     3721 aggcttcaga actatttcaa aaaaagctat gcagcagatt taccacatga tgctgtgctt
     3781 gtagtaactg gctttgaaaa tgcgcaaatt gccgatatga gaagcggaaa catagcgatt
     3841 acgttttcgg gaaggactgt agacattcca tttatgctcg ataagagtgg taaatatgct
     3901 gttttaggaa acccaatcga tactgcaaca tttgaagaga ctccgattaa gggaataaga
     3961 aagggatcta ttacaattgc cagaaataca tatcctatgc ttctgactca ggattctaaa
     4021 tatcttattg caggtgaagt aatagacacc acagtagata aaaccaaaga aacgatggat
     4081 aagatttcac ttgatgacgt accaataaag ggtaacaaga acgcaaaggt tacaatagtt
     4141 gagtattcag atttccaatg cccgttttgc aatagagcca atccaattat cgaaaatgtt
     4201 ttaaaagatt ataaagataa gataagagta gtttataaac agtaccctct tccaaatcat
     4261 aactgggcaa cacctgcatc tatcgcttcc atttgtagct ataaaacagc cgggcatgaa
     4321 gcattttgga aatatcatga caaaattttt gaaaaccaaa gaacgatcac tctggaaaat
     4381 tcagcacaaa agttcgcaga gtttgcaaaa gagcttaaaa ttgatgaaaa gaaattcggc
     4441 gaatgcatgt ccgatcccaa gattgccgag agagttaata acgaaatgtc agaaggacag
     4501 tcaattggaa ttaattccac cccatctttt gtggtaaatg gaattatggt taggggcgca
     4561 aatgaacagg gacttaaatc tgctatcgaa tccagcctta actaatagaa ttcactaaac
     4621 aaacaaaaaa ggcccttaac atttaagggc ctttttttta actaatgtca ggcacctgac
     4681 tatctattaa gtactacctt ttttaataat tgctatctga acacaatctt tattttgtta
     4741 ttacacctat tgtatttaat tgaattaaca ggattaattt ccaatattga aaattaatac
     4801 acccaaaaca ataatcggca ggaaagacag ggtagacttt cctaagctta aactatataa
     4861 catcgatgct aaagtagata cgggtgctta taccacatct attcactgca gtactataga
     4921 accagtaaaa gaaaataata agaagtatgt gagatttaag cttctggact ccactcatcc
     4981 taagtatcaa aagaaaactt ttaaactccc tgtatataag gagaaaaccg taaagaactc
     5041 gtttggagaa agtgagctta ggtttatcgt taaatctatt attagacttt ttgataagga
     5101 ttatgaggtt gaactttcac ttacaaacag agaaaaaatg agctgccctg tgctgattgg
     5161 aagaaaacta ctaaaacacg actttattgt agacataaac agatacaact tatcttataa
     5221 aggaaagatt aaaaagtagc acaaatgaaa atagtcatca tgtccagagg ccccagactt
     5281 tacagtacca ggagattgct ggaagcatgc agggaaagag gacacaaagc aggagttttt
     5341 gatacaacga agttttcaat aaatgtagaa tccggtgatc ctaacttatt atataagaat
     5401 aaatatcttc cgaaatatga tgctgttatt ccccgcatag gggcttctct cacctttttt
     5461 ggaactgctg tactcagaca gtttgaagaa atgggtttat actgtcttaa ttcgtctctt
     5521 ggtatacttt cttcgagaga taaactccgc tctattcaat tacttgcaaa gcataacctg
     5581 ggatttccac atacaatatt tgtaaagaat aaggaaatga taattccagc cattgaagag
     5641 ctcggagggg ctcctgtaat aataaagctg ctcgaaggca ctcagggcat aggcgtcata
     5701 ctcgctgata ccaataaagt agcagaagca atcattgaaa ccctgcagag tacaaaacaa
     5761 aacgtattaa tacagaaatt cgttaaagaa agtaaaggtg tagacattag ggcaatagta
     5821 gcgggcaaca aagttattgc agctatgcgc agaaaagcaa aaggcgatga atttagaagc
     5881 aacgttcaca ggggggggac tgtagaaaaa gtagaactgg atgacaaata tcaacagact
     5941 gcgatacgag ctgctcaaat tatggggctt agtgttgccg gagtagatat gcttgaagga
     6001 aacgacgggc cgcagattat ggaggtaaac tcttctcctg gttttgaagg tatagaaaga
     6061 gcaacgaata tagatgttgc aggcgctata gtagaacacc ttgaacaaca ggctctcttt
     6121 cccgagcttg atctaagaca gaggtttacc ctggataaag gctacggggt tgttgaaatt
     6181 cagatagcaa agaattcaga cattattggg acagcagtta aagatgcggg atttagagag
     6241 aaagacatag ttataatgag cattaagcgc agtaacaaga tgatctcaaa cccaaagtcg
     6301 actcgtgaaa ttttgctggg agatacgctt ttgtgctttg gaaaactcga gaacatgaaa
     6361 tccttgatcc tggaaaatcg aatcaaaaga agaaaaccaa ggaagaaaaa agttgttaac
     6421 aatgaggtgc aaatttaatg ccccatattt tttctataaa cggtaaaaaa gttgcgctag
     6481 gcgaaaatgc ccgaatcgat ctaaagatag cgaaacttcc aactcacact gtaatagacc
     6541 ttccggttta tatacgcagg tcacaaaccg acggacctgt tcttcttata acagctgggc
     6601 ttcatggtga tgagttaaac ggtgtagaaa taataagaag attcatatta agtgaagata
     6661 gtattcctac tacaggaact cttgttatac tacccatagt taatgtttac ggtttcttaa
     6721 acaactctcg tactatgccc gatggcagag acctaaacag gagttttccc ggcactaagg
     6781 agggatctct tgcaggaaga attgcatata cactgatgaa tgtagtatta cctcacgtgg
     6841 attatggtgt ggattttcat acaggggctg taaataaaaa ttatcctcaa ataagatgca
     6901 catttgatat accaaagaat ttagagctgg caaatgcatt ttctcctgca ttcattataa
     6961 attccaagtt aagagacgga tcttttcgca gagcggctca aaaagaggga aagtctatcc
     7021 ttgtttttga aggaggggaa tccctaaggt tcgatgaagt tactattaaa gaaggtattg
     7081 atggaattaa aaggcttatg agctatcttg gtatgaacaa cagtgttttt ataagcaaca
     7141 gagaaactca ggttattaaa caaagttcat ggactagagc aactcactcc ggactttaca
     7201 gaccacttgt agattacggt gatgttgtaa aaaagaaaca ggtaattgca accataaccg
     7261 atccttatgg agaaaccgaa tataagctta agtcacgttt gaacggttat gtactagccg
     7321 ttagaaccct cccaatagta aaccgtggtg atgcaattat gcatattgga accacaaacc
     7381 tggacaagga gcacaactaa aaatgtatct gatcgctgtt attactggta ttagcctgat
     7441 aggctcattt ctctgctccc ttatggaagc tgcattatat tccatgccaa gaagcaggat
     7501 agagagcctt aaaagggatg gtgacccagg agggataagg ctatcgaaac tcagggataa
     7561 aatagacgag ccaatcgcgg ctattcttac tttaaataca gctgttaatg tactgggagc
     7621 atcatgggca ggtgcacttg tatcaaatta ttatggtgac gcatggctgg gacttttttc
     7681 cgccggattc actgcgggag tactattctt tagtgaaata attcctaaat ccttaggagt
     7741 tacttatgca aatgttctag cgccaaagct gtcttccttt atacaacttc ttgttacagt
     7801 cctgatgcct tttgtaaaag ccagtggtta tataacaaga ctgtggggga aaaattcaca
     7861 tttaaacatg cctacagaag aagatatcat aagtcttacg aggttaagca accatggtgg
     7921 agagatactg aagcaggaag ccaaatgggt atccaacgca ctgaaattag atgatgtgta
     7981 tgtagaggat attatgaccc caaaaaatgt ggtttgtact cttgaagaaa ataccacttt
     8041 agaccagatc gatctggatt ctgaccactg gcgttatagc cgtatacctg tatttagtaa
     8101 agaagatgaa aataagattg taggaatagt aagaaggcag gatatatttc aaaacctact
     8161 aaggggtgaa aaagatcagc gaatacacaa tcttatgata gccccggatt ttgttcccac
     8221 caatatgctg cttcatgatc tactggatag atttttgatt actcgtaggc acctgttttg
     8281 tgtcgaaaac aaaaatgaac agtatgtagg tgttgtagca cttgaggatg tattagagtt
     8341 tcttataggc gatgaaattg tcgatgaatt agaccttcat gaagatatgc agaaatattc
     8401 caaaacacaa aaagaagact gatcatatcg aggtatgaat aataggtccg tcataagagt
     8461 gacaaatatt atatattttg tatttggtat attctggtta ttccaacctg tataaattaa
     8521 agatgtttcc ttttaatata tataaagtaa gcgccgtaaa gtattgccaa tacaagcacc
     8581 atccagaaaa tatgttcaat tacagacgtg actccggcgg ttaatccgag caaagctgca
//
LOCUS       DBNGSV010000228        16502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1079394, whole genome shotgun sequence.
ACCESSION   DBNGSV010000228 DBNGSV010000000
VERSION     DBNGSV010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16502
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1079394"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC159U_08290"
     CDS             complement(<1..465)
                     /locus_tag="AC159U_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337096.1"
                     /translation="MTNLKSVTLNPFPIDVLNDLDLGNADGHRDDLIEESFIQTDSIK
                     SFLKDEHAIIVGSFGTGKSALFKLLKEQSEKLDTFLSDLIVPVEEQVQFNELETLADE
                     YFHTLNIKLRYQLIWKFQICRKISEEIAKLEGFPNNDEENYIRSSHRTAKYTK"
     gene            1060..1260
                     /locus_tag="AC159U_08295"
     CDS             1060..1260
                     /locus_tag="AC159U_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337097.1"
                     /translation="MWIDVLIVASYFVIIMLAITAVAVPTFISNTKEFLNTGLLTISN
                     RNVLNLGLALAVSWIASHIIFH"
     gene            complement(1257..2003)
                     /gene="budA"
                     /locus_tag="AC159U_08300"
     CDS             complement(1257..2003)
                     /gene="budA"
                     /locus_tag="AC159U_08300"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIR3337098.1"
                     /translation="MSTINYLRMKKINNYFITFLSIILIGLITFSCVQHSGDLVYQES
                     TLGKLDDGEMEGMLSVGDLKKHGNFGIGTFDGLDGEMVAVDGEFFQVDYQGESNAAND
                     KIKTPFAMVTFFESDKSLKLTGDFNYKLLRTTIDEILPSKDLIYAIKITGQYTYIKTR
                     SVPKQEKPYPSLKEVVKQQKIFDFNDIKGTAVGFWVPKYMKNINVPGYHFHFITEDKT
                     AGGHVLELQITNPTVHIDFNTDLHLSLFED"
     gene            complement(1966..2502)
                     /gene="def"
                     /locus_tag="AC159U_08305"
     CDS             complement(1966..2502)
                     /gene="def"
                     /locus_tag="AC159U_08305"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3337099.1"
                     /translation="MAKVLEIVELGNPVLRERAKAVEDISDPTVQQLIDDLILTTKEA
                     SGVGIAAPQVGQSLRIFIISSYSNERYPQAPDMVPVPIINPQIISTSEELVKGWEGCL
                     SIPGIRAPVLRHKEVKIRYFDREGQEFEGILEDFVARIFQHEFDHIEGIVFLDRLKDN
                     SEIITENEYYKLFEDEED"
     gene            2718..3722
                     /locus_tag="AC159U_08310"
     CDS             2718..3722
                     /locus_tag="AC159U_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3337100.1"
                     /translation="MSLAQTSIKKNIFFDYDDSSLRQESKTTLNLYIKQLNNNPDLYI
                     RITGYSDSKGSSDYNKKLSQRRVQSVVGYLKEKGIDSQRIKSRSLGSTSRFSQGESEK
                     SLEMNRRVEIVLIEKEIKEPQAVQPGKKEQAQKIHTKEDTVNKTTEIISQQRPKSLVE
                     IENVVTHELKKIAAENIELEVPAQMRQGEVSVVDATISEFFIINLIKRLNNTTYQRIS
                     PVDNIEFSNFKLSSTNFFINKIEGEKLEKWSWEVTPNESGVKSITLSATMDIVSNKSR
                     QTINVPIFLKTVDVQSTPGYYLSKIMSSGLYIVFLVVLALGIFTMVSKKDNTKNIKKD
                     "
     gene            3744..4442
                     /locus_tag="AC159U_08315"
     CDS             3744..4442
                     /locus_tag="AC159U_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR3337101.1"
                     /translation="MNWLDITVIAIFAVFAITGLFRGFVRQVFSILAIGGGLIVATMF
                     FDLAGFMLVQKGFILNSAIANVIGFILVLLFTYLIIQILGWVVTKLIGTLQLSWADRI
                     GGGVLGGLFGVIMSILLIAGLTFFFGEEDPTFKNSVTTPYLKTAYLLIKDSVPDDIDK
                     EFQRARKLIRAKGIVPASKVKEIIVDETKNKVADKSNTDIRQKTDAGKAQATPAEQNE
                     KKDKTNTEEDKIEQ"
     gene            4439..5449
                     /gene="fbp"
                     /locus_tag="AC159U_08320"
     CDS             4439..5449
                     /gene="fbp"
                     /locus_tag="AC159U_08320"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR3337102.1"
                     /translation="MIGIETLDEFVIRRQDNFPDASGELSRLLRDIGLAGKIVQREVN
                     KAGLVDILGDTGSVNVQGEKVKKLDVFANNRFIAALKMGGACCGIASEENENFIIIDD
                     ELSQNAHYVVALDPLDGSSNIDVNVSVGTIFCIYKRVSLPGGLCQLADFLQQGTRQVA
                     AGYILYGSSTMLVYTTGHGVNGFTLDPTIGEFCLSHPDITIPNSGTTYSVNQGNYSKF
                     TKGVQKFISYCSDDDKKTNRPYSLRYVGSLVADIHRNLLKGGIFMYPSTSSLPKGKLR
                     LLYECNPMAFIVQQAGGRAINGKEDVLGLDPKDLHQRTPMYIGSTDLVNKLEEFIDKT
                     SE"
     gene            complement(5467..6525)
                     /locus_tag="AC159U_08325"
     CDS             complement(5467..6525)
                     /locus_tag="AC159U_08325"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3337103.1"
                     /translation="MAKAKNTISILGSEADYYLNHKCKTIAKSKLYLPGPNYIDEVFS
                     QNDRPNAVLRNLQSLFNTGRLARTGYLSILPVDQGIEHSGGASFAPNPIYFDPENLVK
                     LAIEGGCNAVASTLGVLGAVSRKYAHKIPFVVKLNHNELLTYPNKFDQVLFANVDQAF
                     AMGAAGVGATIYYGSDESTRQIQEISEAFHYAHSLGLVTILWAYLRNPAFKTKEADYH
                     VSSDLTGQANHLAATIEADIVKQKQAENNGGYLALKFGKTHDSVYSKLTSDHPIDLTR
                     YQLVNCYMGRAGLINSGGASGSSDLKDAVTTAVINKRAGGMGLISGRKSFQKPMKDGV
                     ALLNAIQDVYLDSQITVA"
     gene            complement(6543..7745)
                     /locus_tag="AC159U_08330"
     CDS             complement(6543..7745)
                     /locus_tag="AC159U_08330"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3337104.1"
                     /translation="MSDKLLVSDLEDSELKGKTVFVRVDFNVPIRDGKISENYRIRRT
                     VPTIDYLVDRGAKVIVASHMGRPRGHVISSLSLEPVAERLAELLGRDVKFVGKAVGRE
                     VKKAVKDLKNGQILVLENLRFHIEERDNEPKFCKQLASLADIYVNDAFGTSHRAHAST
                     FGMAQHFDKRLAGFVVDRELKFLQQLINTPKRPYLVIVGGSKIKDKIHALKNLMEKAD
                     EVIIGGGVAYTFLKAKGINVGNSVTEEEMIPWVTEALQKYGDKIVLPIDHIVAESFEN
                     RKNLIVVDENIPDELHGFDIGPKTASKFISHIRRKHTIFWSGPMGVFEVDDFSSGTTH
                     IARAIALATWRGSTTVVGGGDTTSALRKAEVLISEISHVSTGGGASMEYLGGDELPGI
                     TILNDKTD"
     gene            complement(7742..8749)
                     /gene="gap"
                     /locus_tag="AC159U_08335"
     CDS             complement(7742..8749)
                     /gene="gap"
                     /locus_tag="AC159U_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3337105.1"
                     /translation="MPIKVGINGFGRIGRHVLRIGLQRKNLDFVGINDITDAKTLAHL
                     FKYDSVFGPYNGEVKVSGNYIVIDGKKIRVFSERDPDNIDWNGLKTQQVVESTGIFTS
                     KELASKHMGGSVKKVIITAPSKGEVDFSVVMGVNHKDYKPSKHNIISNASCTTNCFSM
                     VVKVLHDKFGIKRGQMTTVHSYTNDQRILDAPHKDLRRARSAALSIIPTSTGAANLIQ
                     VIFPELKGKLQAVAMRVPTPDVSVVDFVAELKSNTTVEQINAEFKKASKGSLKGFLRY
                     SDEPLVSSDLIGDTHSATFDSLLTSMVGNNLVKVIAWYDNEYGYSSRVVDLIEFAGKK
                     R"
     gene            complement(8879..9502)
                     /gene="hisG"
                     /locus_tag="AC159U_08340"
     CDS             complement(8879..9502)
                     /gene="hisG"
                     /locus_tag="AC159U_08340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3337106.1"
                     /translation="MITIAFSRGRLLEEATAIFKKAGYNINDILKNTRKLVFEYPSIG
                     MKAMVVRPTDVPAYVEYGAADCGIVGKDTLMEEKYNLYEPLDLRIGKCKLIVAAPDGY
                     GRDKSKSLRVATKYPAIASDYFAQQGITVEIVKLYGSVELAPIVGLSDVIVDLTATGE
                     TLQKNKLESIDVISDITARLIVNRVSMKVKSKEIKEFITKLQNVRTN"
     gene            complement(9499..10755)
                     /gene="murA"
                     /locus_tag="AC159U_08345"
     CDS             complement(9499..10755)
                     /gene="murA"
                     /locus_tag="AC159U_08345"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3337107.1"
                     /translation="MEKLVIEGGNKLKGELVISGAKNSVLPLMASTILTSGVNTFTNV
                     PDLADVRTMAELLTMLGAKVEYHKGKLEIDSTGINKWIAPYELVRTMRASVLVLGPLT
                     ARFGKTRVSFPGGCAIGERPVDQHLKGLEILGSKIIIDEGYIETKAKKLSGSIIPFDI
                     STVTGTENMMLASVLAEGETILLNAACEPEVTDLANALNKMGAKITGAGTDIITIKGV
                     NKLKPIKNYKVMPDRIESGTLMVAAVALSADLVLKNCAFENSGAVIGKLIEVGAEITK
                     VKEGVRVKGTGKLKSVDITTLPYPGFPTDMQAQLMTLMSISRGLSIINETIFPQRYLH
                     AGELRRMGADIKLVGSSAVVRGVESLSGAPIMASDLRASASLVLAGLVANGRTDILRV
                     YHLDRGYDRLDKKLEQIGAKIWREKE"
     gene            complement(10743..11585)
                     /gene="prmC"
                     /locus_tag="AC159U_08350"
     CDS             complement(10743..11585)
                     /gene="prmC"
                     /locus_tag="AC159U_08350"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3337108.1"
                     /translation="MNIKELYNFGRQTLSDPYFKNPGLEASLLLHAATGVDKLTYYKD
                     QDRLVSADEIDKYNDLLRRRLSNEPIAYILGKKEFYSREFTVDTNVLIPRPETEILVE
                     QTINIAKEFNHPYILDIGTGSGCIAITIMAEIAACTCVASDISQNTLLKAKKNSVLNN
                     TNPYFIKGSLLGSIKGETFDIIVSNPPYVKEQEFSKLENGVKNYEPKRALIAKDNGLA
                     LISEIINESKRVLKNGGWCLLEIGYDQSDRVVEMFELNGYKETQVFKDLSGIDRVVKA
                     KWKN"
     gene            11678..12277
                     /locus_tag="AC159U_08355"
     CDS             11678..12277
                     /locus_tag="AC159U_08355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3337109.1"
                     /translation="MKHKFILASSSPRRIKMLGDLGINFEVIAPDINESPLESEPPED
                     FVLRLSKEKADYVCSKVSEKAVILAADTVVILGKIILGKPLDEIDAKRMLEMLSGNTH
                     KVATGFTLMDQDCAVLHSEAVFTEVTFKNLAPGEIDGYIKTGEPMDKAGAYAIQGTGS
                     FMVEEINGSFTNVVGLPLSRVVEILSNLGLLKLFSKNGN"
     gene            12267..12968
                     /locus_tag="AC159U_08360"
     CDS             12267..12968
                     /locus_tag="AC159U_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3337110.1"
                     /translation="MGIKEKLEVINERINRAAERSERNTSDITLLAVSKQVESGRIRE
                     AIDSEHLSFGENYVQEFVTKYDELSEHKNIDWHFIGHLQKNKVKYIIDKVSLIHSVDK
                     VSLAGEINKRAEKLGIVMPILVEVNLGGEESKSGIEPDELGSFLEKLSALDNIRLSGL
                     MALPPYCDNAEDSRKYFIKLRELRDNFISSYNTLNELSMGMSHDFEIAVEEGATIVRV
                     GTAIFGERPRKKAYL"
     gene            12994..13812
                     /gene="proC"
                     /locus_tag="AC159U_08365"
     CDS             12994..13812
                     /gene="proC"
                     /locus_tag="AC159U_08365"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3337111.1"
                     /translation="MYKVGFIGAGNMAEALIKGLIGSKVLGKKDITASDISKSRENYI
                     SKKYGVNRSKDNNALAKESEVVVLSVKPNNIKTVVQEISSELTATKILISIAAGVTTN
                     VISNALGKKGKIVRVMPNTPALVLEGASAIYFGTGLTAKDKKQVQKIFGAIGKTFIVE
                     KEQLMNGITALSGSGPAFVSLFIEALSDGGVKTGLPRATALELAVQTVLGAAKLISEE
                     NIHPAKLKDMVSSPAGTTIEGLTELEVNGFRGNVISAIEAAEHRSRELSEEAEK"
     gene            13812..14108
                     /locus_tag="AC159U_08370"
     CDS             13812..14108
                     /locus_tag="AC159U_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3337112.1"
                     /translation="MDIGGTFVVTIIKLIILMLNIYMYLIIFRAIISWVNPDPYNPLV
                     QFLRMATDPPLRFLRRFIPPIGGALDLTPIIVIFVLYFIIELLSQYAYQLSRGF"
     gene            14449..14622
                     /locus_tag="AC159U_08375"
     CDS             14449..14622
                     /locus_tag="AC159U_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014539743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system antitoxin ParD family protein"
                     /protein_id="HIR3337113.1"
                     /translation="MIRLTLGKRNNVEKRSMTSQVEYWAKIGNIAEDNPDLPFSNIRA
                     FLLFRELAKGSIE"
     gene            14852..15172
                     /locus_tag="AC159U_08380"
     CDS             14852..15172
                     /locus_tag="AC159U_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337114.1"
                     /translation="MFIYSDSPHWKDYIEENILPKLGSRCVTFKWSKRKWINIGDKLD
                     NAIFYNWGGYTDFNPIAIIYIGFFNIKTVRFYKAFRDSKHGKDKLLKDRLEELSNIVG
                     IKFG"
     gene            15242..>16502
                     /locus_tag="AC159U_08385"
     CDS             15242..>16502
                     /locus_tag="AC159U_08385"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3337115.1"
                     /translation="MARLRQCNLFINGKEIKPSSRRYFVRENPATEKDFAQIAESSAK
                     DIDKAVTAARKAFGVWSKVPATERAKMLNTIAGVLAKNQEQLALLNTLETGKPIRESG
                     LVEIGGSIRTLEYYAGSYSQLNGETIPINENQISMTVKEPVGVVAHIIPWNFPLLLSF
                     WKIAPALVAGCTIVLKPAELTSCTIFEIAKLCSKAGLPDGVLNIVPGKGEVAGQALVE
                     HPDVDKVAFTGSTSVGKHVMRTCADTVKRVSLELGGKAPCIIFDDADLDNAIEACLRG
                     GFFNQGENCTAVTKLMLHKKIYKKFMDTYIKRIKKINIGDPTKPDTEMGTVISKEHYD
                     SVLNYIARGKADGAKVATGGGRPRKYKKGYFISPTVLENVDPNATVACEEIFGPVVAV
                     IPFSDEDKAIEIANNTEYGLAGGVWTTD"
BASE COUNT         4989 a         3124 c         3177 g         5212 t
ORIGIN      
        1 cttagtatac tttgctgtcc tatgcgagct ccttatataa ttttcttcgt cattattagg
       61 aaaaccttct aattttgcta tttcctctga tatttttcta caaatttgga acttccaaat
      121 taattgatat cttaatttaa tgtttagtgt atgaaaatat tcatccgcta atgtttcaag
      181 ttcattgaat tgtacttgtt cttctactgg tactatcaaa tcacttaaaa aagtatctag
      241 tttttcactt tgttccttta aaagtttaaa taaagcactt ttacccgtac caaatgaccc
      301 gacaatgatt gcatgttcat ctttcaaaaa tgatttaatt gaatctgttt gaatgaaact
      361 ttcttctata agatcatctc tatgaccgtc tgcattacct aaatctagat catttaatac
      421 atctattggg aaaggattca aagttactga tttgagatta gtcattataa gtattaattg
      481 ctatagcatt tttaatgaat ttatacctga gaaatataca atttagaagt aataatatta
      541 ggtagtagcg tacactttta tgtatattaa caatttacag tactttaata ttatatacat
      601 aaagcagaga catttagaaa actcaaaaaa tcaaagtttt ggggttaatg ggatttattt
      661 gagtttttga ttctagtctg ggatttcgta tacacccatc tttattttct tgattttgcc
      721 gcactgggct agatttgaaa ttgctctcgt tatagttgat cttgggtgtc cttcctcctg
      781 acacagggat tggatttcta aagtcgacca cactgttttc ctttccttca gccccaaaat
      841 aaactcctga acaaaaacga gatcctcaca tcatgcaacc tttgatcaat tcttgcggta
      901 gcacgattca ggatgacggg gtgatgggac agtgtttgcc cattgggatg gcttttactt
      961 ttttaattca cattaatttt ttattgatat aactttgata tcatagatta tcaataattt
     1021 aataactgat aaatttatgg taacagaata acagtagata tgtggatcga tgtattaata
     1081 gttgcttcct actttgtgat tatcatgctt gcaattacag ccgtggcggt tcctactttt
     1141 atcagtaaca caaaggaatt tctaaacaca ggccttctta ctatttcaaa tagaaatgtc
     1201 ttaaacctgg ggctcgccct agccgtctca tggatagcaa gccatataat ctttcattaa
     1261 tcctcaaaaa gtgaaaggtg caggtctgta ttaaaatcta tgtgtactgt gggatttgta
     1321 atctgtagtt ccagtacatg accaccggca gttttgtctt ctgtaataaa atgaaaatga
     1381 tatccgggga cattgatatt tttcatatac tttggtaccc aaaatccaac ggccgttccc
     1441 ttaatatcat taaaatcaaa aatcttttgc tgcttgacca cttcttttag tgacggatag
     1501 ggtttttcct gcttgggaac gcttcttgtt tttatgtacg tatactgccc tgttatcttt
     1561 atagcataaa taaggtcttt tgagggaaga atttcatcaa ttgtagtcct aagaagctta
     1621 taattaaaat cacctgtaag ctttaaagac ttatccgact caaaaaaggt gaccattgca
     1681 aatggggtct ttattttatc atttgcagca ttggactctc cctggtaatc gacctgaaag
     1741 aattctccgt caactgctac catctctcca tcaagcccat cgaatgtacc aataccaaaa
     1801 tttccatgtt ttttaaggtc acccacagag agcatcccct ccatctcgcc gtcatccaat
     1861 tttcccagtg ttgattcctg ataaaccaga tcaccggagt gctgtacgca tgaaaaagta
     1921 ataagaccta ttaaaataat tgatagaaat gttatgaaat aattattaat cttcttcatc
     1981 ctcaaatagt ttatagtact cattttctgt gatgatctcc gaattgtctt tgagcctgtc
     2041 caaaaacact ataccctcga tatgatcaaa ttcgtgctga aagattcttg caacaaagtc
     2101 ttccaatata ccctcaaact cttggccttc cctatcgaaa tatctgattt tgacttcttt
     2161 gtgtctaagc actggggctc tgatcccggg gatactaaga cagccttccc agcccttaac
     2221 aagctcctcg gaagttgaaa taatttgtgg attgattatt ggtacaggga ccatatcagg
     2281 ggcttggggg tatctctcat ttgaatagga tgagattata aaaatcctaa gagactgccc
     2341 gacctgggga gctgctattc ccactccgct tgcttccttt gtagtaagta taagatcatc
     2401 aataagctgc tgtactgttg gatcagatat atcctctact gcttttgccc tttctctcag
     2461 aacaggattt ccaagttcta ctatttcgag tacttttgcc attttactat aagttaatga
     2521 attttttgaa aaaataaacc aagccaaaaa aacattgaaa aactgagact tcaatgcaat
     2581 aattaaaaag attgttttcc ctttatgaag ttatataatt tcatcttaaa aagaggttta
     2641 ataaccaact gaatatttta ttaaacacaa aaatcatttg catagtctgt gtactgtttg
     2701 tcattataag cgctaatttg tccttagcac agacttctat taaaaaaaat attttttttg
     2761 attatgatga ttcatctctg agacaggaat caaaaactac tctgaatctt tatattaaac
     2821 aattaaacaa taatcctgat ttatatataa gaattacggg ttattcggac agcaagggca
     2881 gttctgatta taataagaaa ctctcacaga ggagagtaca gtctgtagtg ggttatttga
     2941 aagagaaggg aatagactca cagaggataa aatcaaggag tcttggaagc accagcaggt
     3001 tttctcaagg tgagtctgaa aagtctttag aaatgaacag aagggttgag attgtgctta
     3061 ttgaaaaaga gataaaagaa ccgcaagcag tacagcctgg taaaaaagag caggcgcaaa
     3121 aaatacatac aaaagaagat acggtgaaca aaactacaga gataataagc caacaaagac
     3181 ctaaaagcct tgttgaaatt gaaaacgtag ttacccatga gcttaaaaaa atagctgcag
     3241 aaaacattga gctggaagta cctgctcaga tgagacaggg cgaggtaagc gtagtcgatg
     3301 ccacgatttc ggagttcttt atcataaatc ttataaaaag actgaataac actacatacc
     3361 agaggatatc acctgtagat aatatagagt ttagcaactt taagctctct tctacaaatt
     3421 tttttattaa taagatagaa ggggaaaaac ttgagaaatg gtcctgggaa gtaacaccaa
     3481 acgaatctgg ggtaaagagt attacgcttt cagctacaat ggacattgta tcaaacaaat
     3541 cccgacaaac cataaatgtt ccaatttttt taaaaaccgt agatgtacag agcacccccg
     3601 gctattattt atcaaaaatt atgtcaagtg gtctatatat agtattctta gttgtacttg
     3661 cgcttggtat atttacaatg gtttctaaaa aggataatac aaagaatatt aaaaaggact
     3721 aattttataa tactactatg ggaatgaatt ggcttgatat aacagttata gcgatattcg
     3781 ctgtgtttgc gatcacggga cttttcagag ggttcgtgcg acaagtgttt tcgattcttg
     3841 ccattggcgg ggggcttata gtagctacga tgttttttga tctcgccggt ttcatgcttg
     3901 tacaaaaggg atttatacta aactcagcga tagcaaacgt aataggattt atactcgttc
     3961 ttctctttac atatttaata atccagattc ttggctgggt tgtaacaaag ctcataggca
     4021 cactacagct aagctgggcg gacaggattg gcggtggggt gcttggtggg ctgtttgggg
     4081 ttattatgag tatacttcta atagcggggc ttacgttctt tttcggtgag gaggatccta
     4141 cattcaaaaa ctctgtgact acgccgtatc taaaaacagc ctaccttctg attaaagatt
     4201 ccgtaccgga cgatattgat aaggagtttc agcgggcaag aaagctaata agagcaaagg
     4261 gaattgtacc tgcatccaag gttaaagaaa ttattgttga tgaaaccaag aataaagtag
     4321 cagataaatc caatactgat attagacaaa agacagatgc cggtaaggca caagcaacac
     4381 ctgctgaaca aaatgaaaag aaggataaga caaatactga ggaagataaa atagaacaat
     4441 gataggaata gaaacgttag acgaatttgt tataagaaga caggacaatt tcccagatgc
     4501 atctggtgag ttatcaagac ttctgagaga cataggactt gcgggcaaga ttgttcaaag
     4561 agaggttaat aaagcgggtc ttgtggatat attgggtgat acgggatcgg tcaatgttca
     4621 gggtgaaaaa gtaaaaaaac tggatgtttt tgcaaacaac aggtttatag ccgcgcttaa
     4681 gatgggtgga gcatgctgcg gtatagcgtc ggaagaaaat gagaatttca taataataga
     4741 cgacgagctc tcacagaacg cacattacgt agttgccctt gatcctctgg atgggtcttc
     4801 aaacattgat gtaaatgttt cagttggaac aattttttgt atatacaaga gagtttcact
     4861 ccccggaggt ctttgtcagc ttgcagattt tctgcagcaa ggcactcggc aggtagccgc
     4921 agggtatata ctttacggct catcgactat gcttgtttat actactggac acggtgtgaa
     4981 tggatttacc ctcgatccta caattggtga gttctgcctt tctcatcccg atatcacaat
     5041 ccctaattcg ggaactacct attctgtaaa tcagggcaat tattctaaat ttacaaaagg
     5101 cgtacaaaag tttataagct actgcagcga tgacgataaa aaaaccaacc ggccctattc
     5161 ccttagatat gtaggatctc ttgtagccga tattcacaga aatttgctaa agggcgggat
     5221 attcatgtat ccctcaactt cgtctcttcc taaaggaaag cttagactcc tttatgaatg
     5281 caaccccatg gcctttatag tacaacaggc cgggggaagg gcaattaatg ggaaagagga
     5341 tgtattagga ctagacccca aagaccttca tcagagaacc cctatgtata taggctcgac
     5401 agacctcgtt aataagcttg aagaatttat agataaaaca tccgaataat atatatctat
     5461 taaggtttaa gccactgtaa tttggctatc cagataaaca tcctgaattg catttagtaa
     5521 ggctaccccg tctttcattg gcttttgaaa agattttctt ccacttataa gtcccattcc
     5581 gcctgcgcgt ttatttatta cagccgtagt tacagcatct ttgagatcac tgctgcctga
     5641 tgctccgcct gagtttataa gaccagctct tcccatgtag cagtttacaa gctgatacct
     5701 tgtaagatca atcggatggt cgcttgtaag cttcgagtat acactgtcgt gggtctttcc
     5761 gaatttgagc gcaagatagc cgccattatt ctcggcctgt ttctgcttca caatatcggc
     5821 ttcaatagtt gcagctagat gatttgcctg tcctgtgagg tcagaagata catgataatc
     5881 ggcctctttg gttttaaaag cagggtttct aaggtatgcc catagaattg ttacaagccc
     5941 aagagagtgt gcatagtgaa atgcctcaga gatttcctgt atctgtcttg tgctttcatc
     6001 cgagccgtag tagatagtag ctcccacgcc tgcagccccc atagcaaaag cctgatcaac
     6061 gtttgcaaac agcacctgat caaatttatt tggatatgtg agaagctcat tgtggtttag
     6121 ctttactaca aaaggtatct tatgtgcata ttttctggac actgcaccca gtacaccaag
     6181 agtactggca actgcattac aaccgccttc tattgcaagt tttacaagat tttcagggtc
     6241 gaaataaatc gggtttggcg caaatgaagc gccgccggaa tgttctattc cctgatctac
     6301 ggggagaatt gagagataac cggttcgggc aaggcgacct gtattaaaca gagactggag
     6361 atttcttaaa acagcattag gcctgtcatt ttgactgaat acttcatcta tgtagtttgg
     6421 ccccggaagg taaagcttac ttttagcaat tgttttacat ttatggttta aatagtagtc
     6481 tgcttcacta cctaaaatac ttatagtatt ttttgctttt gccattttag cttctcccta
     6541 atttaatctg ttttatcgtt taaaattgtt atgccaggaa gttcatctcc tcctaaatat
     6601 tccatagatg caccgccacc tgtggataca tggcttattt ctgaaataag cacttctgcc
     6661 tttctaagcg cagaggtagt atcccctccg cctacaacag ttgtagaccc gcgccatgtg
     6721 gcaagtgcaa ttgctcttgc tatatgagta gttcccgacg aaaaatcatc aacctcaaat
     6781 actcccatag gtccgctcca gaatatggtg tgtttgcgtc ttatatgtga tataaacttt
     6841 gaagcagttt ttgggccgat atcaaacccg tgaagctcat ctggtatatt ctcgtccaca
     6901 actattaaat tttttctgtt ttcaaaactt tcagcaacta tatgatctat aggaagtact
     6961 attttatcac catatttttg cagggcttca gtcacccatg ggatcatttc ctcttctgta
     7021 actgagttgc cgacatttat gccctttgcc tttaaaaaag tatatgctac cccgccacca
     7081 ataataacct catctgcctt ttccataaga tttttcagtg catgtatctt atctttaatc
     7141 tttgatccac cgactattac aagatatggt ctttttggtg tattaataag ctgttgcaaa
     7201 aactttagtt ctctgtctac tacaaaaccg gctagcctct tatcaaaatg ctgtgccatt
     7261 ccaaatgtag aagcatgggc tctgtgtgag gtgccaaatg catcatttac atatatgtct
     7321 gcaagagagg ctaattgttt gcaaaattta ggctcgttgt ctctttcttc aatgtgaaac
     7381 ctaagatttt cgagaaccag tatttgtccg tttttaagat ctttgactgc tttttttact
     7441 tctcttccaa ctgctttacc tacaaatttt acgtctcttc ccagtagttc agcaagtctt
     7501 tctgccacag gttcaagtga taggctcgaa attacatgac ctcttggtct gcccatatga
     7561 gaggctacaa taacttttgc gcctctgtct acaagataat caatagtggg tactgttctt
     7621 ctaattctgt aattttcgct tatttttcca tctctgatag gtacattaaa atccacacgc
     7681 acaaagacag tcttgccttt taattcagaa tcttctaagt cagaaactaa cagtttatcg
     7741 ctcaacgctt tttacctgca aactcaatca gatcgactac tcttgatgaa tagccatatt
     7801 cattatcata ccatgctatg actttaacta aattatttcc taccattgaa gtaagcaggg
     7861 agtcaaaagt agcggagtgt gtatctccta taagatcgga agatacaagt ggctcatccg
     7921 aatacctgag aaaacctttg agacttcctt ttgatgcttt tttgaattca gcgtttatct
     7981 gctctactgt tgtattgctt ttaagctcgg caacaaaatc aactacggac acatccggcg
     8041 ttggcactct cattgccact gcctgcagtt tgccttttaa ttctggaaaa atgacctgta
     8101 tcaaatttgc tgcgccggta ctggtaggaa ttattgaaag agcagccgaa cgcgctcttc
     8161 ttaaatcttt atgaggcgca tccaaaatcc tttggtcatt tgtatatgaa tgaactgtgg
     8221 tcatctgtcc gcgttttatg ccaaatttat catgaagtac ttttaccacc atactaaagc
     8281 agttagtagt acaagaggca tttgagatta tgttatgttt ggaaggtttg taatccttgt
     8341 gattaactcc cattacaact gagaaatcta cttcgccttt tgatggtgca gtaataataa
     8401 cctttttaac cgagcctccc atgtgtttgg aagctaactc tttactggta aaaattcctg
     8461 tagattccac aacctgctga gttttaagtc cgttccagtc aatattatcg ggatctcttt
     8521 cagaaaatac ccttattttt ttaccatcaa ttactatata atttccgctt acttttactt
     8581 caccgttata agggccgaat acagaatcgt atttaaacag gtgtgccaga gttttagcat
     8641 ctgtaatatc atttataccc acaaaatcca gattttttct ttgcaggccg attcttagta
     8701 cgtgtcttcc tattcttcca aatccgttaa ttcctacctt aataggcatt taattcctcc
     8761 aatttgctgt aaatttaaga tattgctact gtcaaagccg aaaaactacc caaatgtcaa
     8821 tcagagtcaa gttgctgtat atgctagttt ttttattatt aagttgattt ctgatcaatt
     8881 aattcgttct tacattctga agctttgtta taaactcttt tatctcttta gacttaactt
     8941 tcatgcttac tctgtttact atgagccgcg cagtaatgtc ggatatcaca tcgatagatt
     9001 ccagtttatt tttctgaagg gtttcacccg tggctgtaag atcgacaatc acatcagaaa
     9061 gtcctacaat cggagcaagc tcaacagagc catacagttt tactatttca acggtaatcc
     9121 cctgctgagc aaaatagtca cttgctattg caggatattt tgttgctacc ctaagggact
     9181 ttgatttatc ccgcccataa ccatcgggag ctgctacaat caatttgcat tttcctattc
     9241 ttaaatctaa tggttcatat agattatact tttcttccat aagggtatct tttcccacga
     9301 tgccgcagtc tgcagcccca tactccacat atgctgggac atctgtagga cgaacaacca
     9361 tggctttcat accaatactt gggtattcaa ataccagttt tcttgtattt tttagtatgt
     9421 cattaatgtt gtagccggct tttttgaata tagctgtagc ttcttcaagc agtcttccac
     9481 gtgaaaatgc aattgtgatc attctttctc cctccaaatt ttagcgccaa tctgttctag
     9541 ttttttatcc aatctgtcgt agccacggtc taagtggtat accctcaata tgtcagtcct
     9601 gccgtttgca acaagccctg caagtacaag agaagcgctt gctcttaaat cgcttgccat
     9661 tattggcgcc ccgcttaggc tttctacacc ccttacaaca gcgctgcttc caacaagctt
     9721 aatgtccgct cccattcttc taagttcccc ggcatgcaga tatctctgag gaaatattgt
     9781 ttcatttata atactaagac ccctggaaat gctcattaaa gtcattaact gtgcctgcat
     9841 atcggtagga aatccagggt agggaagagt tgtaatatct acacttttta atttgcctgt
     9901 gccttttact cttactcctt ccttaacttt tgtaatttca gctcctacct cgattaattt
     9961 tcctattaca gccccactgt tctcaaatgc gcagttttta agcacaaggt cagcagaaag
    10021 cgcaactgct gcaaccataa gagtaccgct ctctattctg tccggcatta ctttatagtt
    10081 ttttattggc ttgagtttgt ttactccttt gatagtaatt atgtcggttc cggcacctgt
    10141 gatctttgcg cccattttat tgagtgcatt tgcaaggtca gtaacctctg gctcacatgc
    10201 tgcgttaagc aagatagttt ctccctctgc aagcaccgat gcaagcatca tattctcggt
    10261 tcctgttact gtagaaatat caaaggggat aatactgcca gataactttt tagcctttgt
    10321 ttctatatat ccttcatcta ttattatttt tgatccaagt atctctagtc cttttaggtg
    10381 ctgatccaca ggtctttcgc ctatcgcaca gccaccggga aaggaaactc tggttttacc
    10441 aaaacgtgca gttagggggc ccaacactaa tacggatgct ctcatggttc ttacaagttc
    10501 atatggagcg atccacttat ttatgcccgt ggagtctatt tccagttttc ctttatggta
    10561 ctctaccttg gctccaagca tggtaagcag ttctgccatt gtacgtacgt cagctaagtc
    10621 cggcacattt gtaaatgtgt ttaccccgct tgtaaggatt gttgaggcca taagaggaag
    10681 tacggagttt ttagctccgc taatgactaa ctcgccctta agcttgtttc cgccttctat
    10741 tactagtttt tccattttgc tttgactacc ctgtctatgc cagataaatc cttaaatacc
    10801 tgggtttctt tataaccatt taattcaaac atttcaacca ctctatccga ttgatcatac
    10861 cctatttcga gcagacacca tccgccgttt ttcaaaaccc tcttggactc atttattatt
    10921 tcactaatta gagccagtcc gttgtccttg gctattagag ctctctttgg ttcataattt
    10981 ttaacaccgt tctcaagttt tgagaattct tgctctttta catagggtgg atttgataca
    11041 atgatatcga atgtttctcc ctttatagac ccaagcaggg agcctttaat aaagtaggga
    11101 ttagtattgt tcaagaccga atttttttta gctttaagca gcgtgttttg agatatatcg
    11161 cttgcaacac aggtacaagc agcaatctct gccataattg ttatagctat acaaccacta
    11221 cctgtgccaa tatccaggat gtatgggtgg ttaaattctt ttgctatgtt aattgtctgt
    11281 tctacaagaa tctctgtttc aggtcttgga atcagtacat ttgtatctac tgtaaactct
    11341 ctggaataga attctttttt gccaagtata tatgcaatag gttcatttga cagtctgcga
    11401 cgtagcaaat cattatactt atcaatttca tcagcactta ccaacctatc ctgatcctta
    11461 taataggtta atttatccac cccggttgct gcatgtaaaa gcagggatgc ttctagaccc
    11521 gggtttttaa aatacggatc acttagtgtc tgccttccaa aattgtataa ttcttttata
    11581 ttcataagga tagttcagta tacataatca tttttctaat catatatgta ctctaatata
    11641 ataaggtttt ttctcttgaa gattaaaaat ggctcatatg aaacataaat ttatactcgc
    11701 atcgtcttct cccaggagga tcaagatgct aggtgattta ggaatcaatt ttgaagtaat
    11761 tgctcctgat attaacgaat cccctcttga gagtgaaccg cctgaagatt ttgtattaag
    11821 gttatccaaa gaaaaagcag attatgtatg ttctaaagta agcgaaaaag cagttattct
    11881 tgcagccgac acagttgtga tcctggggaa gattattctt ggaaaaccgc tggatgaaat
    11941 tgatgcaaag cggatgcttg agatgctttc aggaaatacg cacaaagttg caacagggtt
    12001 tacattaatg gatcaggact gtgctgtatt acattccgaa gccgttttta ctgaagtaac
    12061 atttaagaac cttgcacccg gtgagataga tggatacatt aaaactggtg aaccaatgga
    12121 taaggcaggg gcatatgcca ttcagggcac aggctctttt atggtagaag aaattaacgg
    12181 gtcgtttaca aatgtagtag gactgccgct atccagggta gttgaaatac tttcaaatct
    12241 aggattatta aaactattta gcaagaatgg gaattaaaga gaaattagaa gtaataaatg
    12301 aaagaataaa ccgggccgct gagagaagcg aaaggaatac ttcggacatt acactcctgg
    12361 ctgtttctaa gcaggttgaa tccgggcgta tacgagaggc tatagactct gaacatttga
    12421 gtttcggtga gaattatgtg caggagtttg taaccaaata tgacgagctt tcagaacata
    12481 aaaatataga ctggcatttt ataggccacc tgcaaaagaa caaggttaaa tatattattg
    12541 ataaagtgag tcttattcat tcagtcgata aggtgtcgct tgccggggaa attaataaac
    12601 gagcagaaaa gttgggaatc gtcatgccta tactggttga ggtaaacctt ggtggagagg
    12661 aatcaaagag cgggatagag cctgatgaac tgggcagctt tctggagaag ttatctgcat
    12721 tggataatat tagattatcc ggactgatgg cacttccgcc gtattgtgac aatgctgagg
    12781 attctagaaa atattttatt aaattaagag agctaagaga caactttatt tccagttata
    12841 atacattaaa cgagctttcc atgggaatga gccatgattt tgagatagcg gtggaagaag
    12901 gcgctacgat agtgagagtg ggtaccgcaa tatttggaga gagaccgcgt aaaaaagcgt
    12961 atctgtaaat gtaaattcgt agaggttata taaatgtata aagtaggttt tataggtgca
    13021 ggaaatatgg cagaagctct tatcaagggg cttatcggct ctaaggttct cggcaaaaaa
    13081 gatattactg caagcgatat ttcaaaatca agagagaatt atatatccaa aaaatatggc
    13141 gtaaatagaa gtaaggataa caatgcccta gctaaagagt ctgaggttgt ggttttaagc
    13201 gtaaagccaa acaatatcaa aactgttgta caggagatat catctgaatt gactgcaaca
    13261 aagattttaa tctcaatcgc tgcgggtgtg actactaatg ttataagcaa tgcccttggc
    13321 aaaaaaggaa aaatagtgag ggtcatgccc aatactcctg cacttgtttt agaaggtgct
    13381 tcagcaatat attttggaac cggtctcaca gcaaaagata aaaagcaggt gcagaaaata
    13441 tttggagcaa ttgggaaaac atttatagtg gaaaaagagc agcttatgaa cggtataacg
    13501 gctcttagcg gaagcggtcc tgcgtttgta tcactattta ttgaagcgct ttcagatggt
    13561 ggagtaaaga caggacttcc aagagccaca gcgcttgaac ttgctgtaca gactgtgctt
    13621 ggtgcggcta aattaatttc tgaagaaaat attcatcctg caaaattaaa ggacatggtc
    13681 tcatctccgg cggggactac tattgaaggg cttacagaac ttgaagtaaa tggttttaga
    13741 ggaaatgtga tttcggcaat cgaggctgct gagcacagat caagagaact ttcagaggag
    13801 gcagaaaagt aatggatatc ggcggtactt tcgtagttac aataattaaa cttattatac
    13861 ttatgctaaa tatatatatg tatctgatta tatttagagc tatcatttcc tgggtaaatc
    13921 ctgatcctta caatccactg gttcagtttt taagaatggc tacagaccct cctcttagat
    13981 ttctaagaag atttatccct ccaatcggag gagcattaga cctgacgcca ataatagtaa
    14041 tattcgtact ctattttatc atagaacttc tttcgcagta tgcttatcag ttgtcgaggg
    14101 gattctagaa atgctgtaat gattaaggat gtttactaca aatgcaatgc cctaactgtg
    14161 ctaataatat ttattggatt aaagtaatat taataacacg ctggacatca ataaaatgct
    14221 caaaatgtac tattgagtta aacagaaaac tcgatactaa ttttttccta ctttgctctt
    14281 ttagtatagc agtgactatc tttattgtag aggcgtccct aagcatgtta cttaaagtta
    14341 tactgatatc ttgtttgata gtgattagta cttacatcga ttctatgatt attaagttat
    14401 atcgatcaag gaaaaataaa aattctttgc tcggatataa cataatcgat gattagattg
    14461 acactgggta aacgaaataa tgtagaaaaa cgatctatga caagccaagt ggaatactgg
    14521 gccaaaattg gcaatattgc agaagacaac cccgaccttc ccttttccaa tatacgtgca
    14581 ttcctcttat ttagagaact agccaagggc agcattgaat aatacgtctt cggacaggaa
    14641 ttaatgtatt atctgaaggg ttattgagtt tatattaagt taatcaaagt aattttaaat
    14701 gcttagggat aaattaacag acttaatcca ggcttttcta tttcttttat ttctgccgat
    14761 tctactttta tatctaatat ttaattttct atatgtaact tgtttgaggg ttggatttct
    14821 acttaaatgg aagaggaaag gcaaggatat aatgttcatt tattcagaca gtccacattg
    14881 gaaagattat atagaggaaa atatacttcc aaaactaggc tcacgttgtg taacatttaa
    14941 atggtctaaa aggaaatgga taaatatcgg agataagctt gataatgcga tattttataa
    15001 ctggggtggt tatacagatt ttaatcctat agctatcata tacattggtt tttttaatat
    15061 aaaaacagtc aggttttata aagcattcag agatagtaag catggtaagg ataaattact
    15121 caaagacagg ctagaagaat tatcaaacat agttggtatt aaattcggtt aactattctt
    15181 tatatgacat aatgaaagca ttactatata attttatgta attaataatt aggagaaata
    15241 catggcaaga ctaagacaat gcaatctttt tataaatgga aaagaaataa aaccatccag
    15301 caggagatat tttgtaaggg aaaaccctgc aactgaaaag gactttgcgc agattgctga
    15361 aagcagtgca aaggatatag acaaagcagt aactgctgcc agaaaagcat ttggtgtatg
    15421 gtcaaaagta cctgcgacag aacgtgcaaa gatgctaaac actattgccg gtgtactggc
    15481 taaaaaccaa gaacagcttg cgctgttaaa tacacttgaa accggaaaac ctataagaga
    15541 gagcggactt gttgagattg gcgggtcgat aagaacactt gaatactacg caggttccta
    15601 ctctcaacta aacggcgaga ctattcctat aaacgaaaat cagatatcga tgacagtgaa
    15661 agaacctgtg ggtgtcgtgg ctcacataat tccatggaac tttccgcttc ttttatcatt
    15721 ttggaaaata gcccctgcgc ttgttgccgg atgcacaatt gtgcttaagc ctgccgagct
    15781 tacttcatgc actatttttg agattgctaa attatgcagc aaagccggtc tgcccgatgg
    15841 agtattaaac attgttcccg gaaaaggaga ggtagccggg caggcgctgg tagaacatcc
    15901 cgacgttgat aaggttgcct tcacgggttc tacttcggta ggaaagcacg tgatgagaac
    15961 atgcgctgat acggtcaaga gagtatcact tgagcttgga ggcaaggcgc cgtgcattat
    16021 ttttgatgat gcagatctgg acaatgcaat agaagcctgc ctgcgcggag ggttttttaa
    16081 tcagggtgaa aactgtactg ctgttacaaa gctcatgctc cataaaaaga tatacaagaa
    16141 gtttatggac acttatatta agagaataaa gaaaattaat attggcgatc ccacaaaacc
    16201 tgacactgaa atggggacag taatatctaa agaacattat gacagcgtac tgaattatat
    16261 cgccagagga aaagccgatg gtgcaaaggt tgccacgggt ggaggaagac ccaggaaata
    16321 taagaagggg tattttataa gtccgactgt tttggaaaat gtagacccaa atgcaactgt
    16381 tgcctgcgag gaaatcttcg gtcctgttgt tgccgtgatt ccatttagtg atgaagacaa
    16441 ggctattgag attgcgaaca atacagagta cggacttgcc ggcggagtat ggacaactga
    16501 ca
//
LOCUS       DBNGSV010000229         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1771888, whole genome shotgun sequence.
ACCESSION   DBNGSV010000229 DBNGSV010000000
VERSION     DBNGSV010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1771888"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC159U_08390"
     CDS             <1..348
                     /locus_tag="AC159U_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3337116.1"
                     /translation="GCGGDRDKEKRPMMGTVAAEVADIVIVTSDNPRSEDPEKIILDI
                     ENGIKQAGLKQHEYFMITDRYEAILKSVQISEENDTIVVAGKGHEDYQIIGNQKIHFD
                     DKQACERALKEIA"
     gene            365..1744
                     /locus_tag="AC159U_08395"
     CDS             365..1744
                     /locus_tag="AC159U_08395"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3337117.1"
                     /translation="MLDTKFILKSTNGELMSEGVDMEFSGISTDSRTIEKGQLFVALK
                     GENYDGHKFICDAINRGAAGVVAEQLCDKSKPANKLYIRVGSTLNSLGNLAKSWREGF
                     ENLKVIAITGSNGKTTTKEMVSAVVSKKYNVHKNTGNFNNLIGLPLTIFNLQPENQVA
                     VLELGMNQFGEIKRLSEICSPDVGIITNIGKAHIGNLGGIEGVKLAKGELVENFNSEQ
                     TFIVNKDDPRVLDIAAGARCKKITYSLSGDADVQATDINTDGLSSTYFTLKIGDKTNL
                     VRLKNIGLHNVSNALCAASAGVIMGIDIEQIADALEKFEFPKMRLEIINTSDGLRIIN
                     DCYNANPDSMKNAINILAELNGDFNRIAVLGDMLELGEDAEKEHKELGKHIASAGIDY
                     LFTYGQLGETIHYGTKGSVMGGSFNDHKIIADQIKHVAKKGDVILIKGSRGMKMENIL
                     RHLGVQEKT"
     gene            1843..>2543
                     /gene="mraY"
                     /locus_tag="AC159U_08400"
     CDS             1843..>2543
                     /gene="mraY"
                     /locus_tag="AC159U_08400"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3337118.1"
                     /translation="MISIVLGGKFIDFLKNRQLKESIRDDGPKTHLSKQGTPTMGGVF
                     IVVSVIIASLLWINLSIMYVWFVLLFTISFAAIGFWDDSMKMKKGRGMSARVKFLIQI
                     ILGVALVMMLVSDGKGFTMSLRLNEISSYSSTSVLLPFFKKAVIDLGPLYIVFAVVVI
                     VGSSNAVNLTDGLDGLAIGSIAVVVGTYLVFSYLSGNIIFARYLQIPYIAGSGELAVF
                     LAAVGGACLGFLWYNS"
BASE COUNT          827 a          371 c          599 g          746 t
ORIGIN      
        1 ggttgtggag gtgaccggga taaagaaaaa aggcctatga tgggaactgt tgctgccgag
       61 gttgctgata ttgtgattgt cacatctgat aatccaagat ctgaagatcc tgagaaaata
      121 atattagata ttgaaaatgg aataaaacaa gcaggtttga agcagcatga atattttatg
      181 ataacagaca ggtatgaagc catacttaaa tcagtacaga tatctgaaga aaatgacacc
      241 atagttgttg cgggcaaagg tcatgaagat tatcagatta taggcaatca gaaaattcat
      301 tttgatgaca agcaggcatg tgaaagagct ttaaaggaga tagcttagtt taagaattgc
      361 taacatgtta gatacaaaat ttattttaaa atctacaaat ggagagctca tgtcagaagg
      421 cgtggacatg gagttttcgg gtatttctac tgactcaaga accatagaga aaggtcagtt
      481 gtttgttgca ttaaaaggtg aaaattatga tggccataaa tttatttgcg atgctataaa
      541 cagaggggca gcaggagttg tggcggaaca attatgtgat aaatcaaaac cagcaaacaa
      601 gctgtatata agagttggtt ctacactaaa ctctttgggc aatcttgcaa aaagctggag
      661 agagggtttt gaaaacttaa aggtaattgc aataacaggc tcaaatggga agacaactac
      721 aaaggagatg gtctctgctg tggtatctaa aaaatataat gtacataaaa acacagggaa
      781 ctttaataac ctcattggac ttcctcttac aatttttaac cttcagccag aaaaccaagt
      841 agctgtgctt gagcttggga tgaatcagtt tggtgagatt aagaggctat cagaaatatg
      901 cagtcctgat gtaggaataa taactaatat tggaaaggca catattggaa atctaggggg
      961 gattgaaggc gtaaaactgg cgaaaggtga gcttgtcgaa aattttaatt ccgagcagac
     1021 cttcattgta aataaagatg acccaagggt attggatata gcagcaggcg caagatgtaa
     1081 gaagattaca tattcattaa gcggtgatgc cgatgtacag gcaactgata taaacactga
     1141 tggccttagt tcaacgtatt tcacacttaa aataggagac aaaacaaatc tcgtaaggct
     1201 taagaatatt ggattgcata atgtatctaa tgcgctatgt gcagcaagtg ccggagtgat
     1261 catgggcatt gatattgagc agattgcaga tgcgctggaa aagtttgaat ttcctaagat
     1321 gaggcttgaa attatcaata ccagtgacgg acttagaatt attaacgact gttataatgc
     1381 aaatcccgat tctatgaaaa acgcaataaa tatacttgct gaattgaatg gagattttaa
     1441 caggattgca gttttgggtg atatgcttga acttggagag gatgcagaaa aagaacacaa
     1501 ggaattagga aaacatatag catctgcagg gatagattat ctcttcacct acggacagct
     1561 tggtgagact attcattacg gtactaaagg cagtgttatg ggcggcagct tcaatgatca
     1621 taaaatcatc gccgatcaga ttaaacatgt agctaaaaaa ggggatgtaa ttttaattaa
     1681 aggttcaaga ggaatgaaga tggaaaatat cctcaggcat ttgggggtac aggaaaaaac
     1741 ctgatgttat attacttata ctctaatttt gacagcgttg taatctttaa cgtatttaaa
     1801 tatattacgt ttcgatcttt cggtgcaggg gtcacgtcat ttttgataag tatagtgctt
     1861 ggtggaaagt ttattgattt tcttaaaaac agacagctta aagaaagtat tagagatgac
     1921 ggtcccaaaa cgcacctttc caaacaaggc actccgacta tgggaggcgt atttatagta
     1981 gtttcggtaa ttatagcttc acttctttgg attaatctta gcattatgta cgtatggttt
     2041 gtgttgcttt tcacgattag ttttgcggca atcggatttt gggatgacag tatgaaaatg
     2101 aaaaagggaa ggggtatgag cgcaagggtt aaatttttaa ttcaaataat ccttggagta
     2161 gctcttgtga tgatgctggt ttcagatggc aaagggttta caatgtcact gagactaaac
     2221 gaaatctcaa gctactccag cacatctgtg ctgctgccgt ttttcaaaaa agccgttatt
     2281 gatcttgggc cgttgtatat agtttttgca gtagttgtga tagtgggatc gtctaatgca
     2341 gtgaatctta ccgatggtct cgatggcctg gcaatagggt ctatagcggt tgttgttggt
     2401 acttacctgg tgttttcata tctgtctgga aatataattt ttgcgaggta tctgcagatt
     2461 ccctatatag caggtagtgg agaacttgct gtatttttgg cagccgttgg tggagcatgt
     2521 cttgggtttt tgtggtacaa ctc
//
LOCUS       DBNGSV010000230         1261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1011081, whole genome shotgun sequence.
ACCESSION   DBNGSV010000230 DBNGSV010000000
VERSION     DBNGSV010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1011081"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC159U_08405"
     CDS             <1..659
                     /locus_tag="AC159U_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337119.1"
                     /translation="TVNLTFADTGDTLSLGFLNKIYLVDTTGGATTLTVPDGDFQGQE
                     VTLSNDGTSTLTLNGTGLSAVGVSEAILLTWIDVSWKIIFIGALSFQDLTVDGTLSVD
                     TIAEKTASAGVSFSNTAKWKKYYKSLSTITYGDLYTIISSWVPSINNILAFHGQAYGQ
                     GFGAGTGAAMTIMRRTSATNILIQYVTPTGTISDINITSGSAALASDKIEIMSNFDAF
                     "
     gene            675..1103
                     /locus_tag="AC159U_08410"
     CDS             675..1103
                     /locus_tag="AC159U_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337120.1"
                     /translation="MAAANNNSFESQLLRKMDSMGDNLTAINERLTVFEGETKTAFME
                     LAGKVKGNHELITRDLSNYVEKDVCSDHREKQGSRLGAIEEKVVLLAYRLEKFEEAKA
                     EKDKTASEAVIAWLPGVLKVVIFAGAFLLATANGWLSFGG"
BASE COUNT          413 a          186 c          279 g          383 t
ORIGIN      
        1 aaactgtaaa tcttactttt gctgataccg gagatacttt atctcttgga tttctaaaca
       61 agatttatct tgttgataca acaggtggtg ctaccacttt aactgttccc gatggagatt
      121 tccaaggtca ggaagtcaca ttgtccaatg atggtacaag tacacttaca ttaaatggta
      181 ctggtttaag tgctgtaggt gtttcagaag caattctact tacatggatt gatgtatcgt
      241 ggaagataat ctttataggt gcactttcat ttcaggacct tacagttgat ggcacactat
      301 cagtagacac tatagcagaa aagacagcga gtgcaggggt tagttttagt aatacagcta
      361 agtggaaaaa atactacaag tctctatcaa ctataacata cggagattta tataccatta
      421 tttcttcatg ggttccttct attaataata ttttggcatt tcatgggcaa gcatatggac
      481 aaggttttgg tgcgggaact ggggctgcaa tgacaatcat gagaagaacg tctgcaacaa
      541 atattttaat acaatatgta acgcctacag gcacaatttc agatataaat attactagtg
      601 gaagtgctgc ccttgcttct gataaaatag aaataatgtc taactttgat gcattttaac
      661 taaaggaaaa ataaatggca gctgctaata ataacagttt tgaatctcaa ctattaagga
      721 aaatggacag catgggagac aaccttacag ctataaatga acgtcttact gtttttgaag
      781 gggaaaccaa aactgccttt atggaactag caggtaaagt aaaggggaac catgagctga
      841 taacaaggga tctgtccaat tatgttgaga aagatgtttg ttccgatcac agggagaagc
      901 agggatcgag acttggggca atagaagaga aagttgttct gttagcatat agactagaga
      961 agtttgaaga agcgaaagca gaaaaagata aaacggcatc tgaagcggtt attgcatggc
     1021 ttcccggtgt tttaaaagtt gttatatttg caggggcttt tttattggct actgctaatg
     1081 gttggcttag ctttggaggt taataagtga tcaggcaaga tgatgttttg ttaaatgcta
     1141 tgagtgatct tccatttgaa gaatatgggt gtgcactatt cgatatccaa tatctatcag
     1201 tgatgtatgg agagaataca tttaaacgag aacattttat attagaatgt gaaaagtgga
     1261 t
//
LOCUS       DBNGSV010000231         6924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1079979, whole genome shotgun sequence.
ACCESSION   DBNGSV010000231 DBNGSV010000000
VERSION     DBNGSV010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1079979"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /gene="purL"
                     /locus_tag="AC159U_08415"
     CDS             <1..1631
                     /gene="purL"
                     /locus_tag="AC159U_08415"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR3337121.1"
                     /translation="ATGVGNPVFYVGSKTGRDGIHGAVMASETFTEESEEKRPAVQVG
                     DPFTEKLLLEACLELFKTDAVVGIQDMGAAGLTSSSTEMAERAETGIEIDVNQIPCRE
                     KGMTPYEMMLSESQERMLIVLKQGKEHVAENIFKKWNLDSSVIGNVIEKRSLILTKDG
                     SVECDLPVDIITNEAPYYDREYSKPEYLSQIKDSVNYKKPDNFNDTVIQLLSSPNLCN
                     KNWVIEQYDHMVRTNTVCPPGSDASIVRIKGTDKSIAMTTNCNSKYCYLTPYTGSMIA
                     VAESARNLACSGAVPLAITDCLNFGNPENPEIMWQFKESINGISKAATFLGTPIVSGN
                     VSLYNQTGNEAIYPTPTIGMVGLIEDSSKIINQWFKNENDLIIQIGNTYEEFGGSEFL
                     NLQNPHLLSRAPELNLESEKKLINILLELIQDGILNCAHDISEGGLITALAESCFTPE
                     GQIGIKIENPLPNSLTEDAFLFSESQSRVIVSLDKKKLAKFEKVLNKHEMEFHLLGTT
                     GGDSLVIENLINMPVKEAFDAWDTGFIELLKNNG"
     gene            1624..3042
                     /gene="purF"
                     /locus_tag="AC159U_08420"
     CDS             1624..3042
                     /gene="purF"
                     /locus_tag="AC159U_08420"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3337122.1"
                     /translation="MAKIREECGVFGIFGHPEASNITYLGLHALQHRGQESAGIVTSD
                     DGLLYEHKEMGLVSDIFTEEVINKLKGNNAIGHVRYSTAGMSHHKNSQPLVINHSKGQ
                     QIAVAHNGNLTNARTLREELESDGSIFQTTTDTEVILHFIAKSKEQTLKKRVIEALLK
                     CKGAYSLLFLTPESIIACRDPYGFRPLVLGKLGNSYIVSSESCSFDLIEADFIREIEP
                     GELLIINEDGIESLKPFPETKSKHCIFEHIYFARPDSYLFGDNVYKVRKEFGRQLAIE
                     QPADADIVIAVPDSGVPAAIGYSEQSGLPLELGLLRSHYMGRTFIEPRQVIRNFGVKL
                     KLNAIRNVIDGKRLVIVDDSIVRGTTSRKIVKMLQNAGAKEVHMRISSPPMKYSCYYG
                     VDTPNDGELIANSLSVEEINKYITSDSLGYLSLSGIIEAVERSNPTTCGSNFCNACFT
                     GSYPEQLVDKTDKLKQLHLFER"
     gene            complement(3060..5765)
                     /locus_tag="AC159U_08425"
     CDS             complement(3060..5765)
                     /locus_tag="AC159U_08425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3337123.1"
                     /translation="MSEDLYSQIEKSYNPKSLEQKWYDLWMEKKYYSTDSESSNKPFT
                     IVIPPPNVTGSLHIGHALNNTLQDIIIRYKRMKGFDALWVPGTDHAGIATQMIVEREL
                     AKENILKEQLGREEFVKRVWKWKEESGGTITNQLKRLGALPDWSREKFTLDNSLSQAV
                     RKVFVDLYNEGLIYKDKRLVNWDVKLKTAVSDLEVDQREVKGKLWYIKYPIENSKKYI
                     TVATTRPETMLGDTAVAVHPQDKRYEKLIGKYVLLPLTDRKIPIVADEYSDPEKGSGA
                     VKITPAHDFNDFEVGKRHDLEMIDIFTESAVLNSNVPDKYEGLSRFKARKQILSDLED
                     LGLLEKDEDIKHFVPYGDRSAEIVEPRLTYQWYVDAKELAKNAIKAFEDGEIKFHPKY
                     WENTFFEWMNNIEPWCISRQIWWGHQVPVWYGPDGKIFVELSESDALKSATLHYGKEI
                     ELTQDTDVLDTWFSSGLWPFTTLGWPEQTVDLKKYYPTSVLITAFDIIFFWVARMIMM
                     GIKFTGQVPFRDVYIHGLIRDEKGEKMSKTRGNVIDPLEIIDEFGADSLRFSLAAMAS
                     QGRDIKLSMSVIKGYRNFINKIWNASRFLMLNLEGYDPNCTYDKNSLSIFDKWILTKF
                     NNIIQETDQDYDNFDYDKVANRVYQFIWGDFCDWYIEISKSKLRSEDNTQRQNTQNIL
                     VKLLVYSLQLMHPFTPFITEEIFSMLRNFGVSIVDNKGNVTESLVVSSYPINNESEIF
                     DDEYKEVEFLKQIVISIRNIRAELDVKPSQKIKIFVNTESEELENLISHNKQFIYELA
                     KVDSIEFIKDEKPQNCVSQVLSGVQIFVPEEGLVDIDNEISRMNKEIEKIDKDLVVIE
                     NKLSNKKFIERAPKEIVDKEKGKLIELTDKKAVIKENIDRLSEKK"
     gene            complement(5799..>6924)
                     /locus_tag="AC159U_08430"
     CDS             complement(5799..>6924)
                     /locus_tag="AC159U_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3337124.1"
                     /translation="LKVSEGDRVKEGDLIADWDPYNTPFISDISGVSKYQDLEEGISM
                     KEQVDEVTGIYKKVVMESKNPDYHPAMIIENAEGGHVQYSLPVGAVIEKNSGEEIFAG
                     DVIAKIPRETAKTKDITGGLPRVAELFEARIPRDPAVVTEIDGIVTYGKDLKGKRRLV
                     VTPEMGEPKEYLVPRGKHVLVRDGERVTAGDQLVDGSVNPHDILSIRGEKALSRYLVD
                     EIQEVYRLQGVKINDKHIESIVKQMLKRVRIKEPGDTTFLVGEAIEKQTFFEENQKII
                     EQGGKPATAEPLLLGITRASLSTESWLSAASFQETTRVLTEAACEGKEDKLRGLKENV
                     IMGRLIPAGTGLPMYRNIDIEVEAPEIPVPLPIEEETQTV"
BASE COUNT         2110 a         1416 c         1179 g         2219 t
ORIGIN      
        1 atgccacagg tgtaggaaat ccggtgtttt atgtaggctc caaaaccgga agagacggca
       61 tacacggagc agtaatggct tcagaaactt tcactgaaga atctgaagaa aaacgccccg
      121 cagttcaggt tggtgatcca tttacagaaa agctgctttt ggaagcctgc ctcgaactat
      181 tcaaaacaga tgcagtcgta ggtatacagg atatgggggc tgccgggctt acgtcatcat
      241 caacagagat ggcagaaaga gcagaaaccg gaattgaaat agatgtaaac caaatcccat
      301 gcagagaaaa aggcatgact ccttatgaga tgatgctttc tgaatctcag gaaaggatgc
      361 taattgtact aaaacaagga aaggaacatg tagcagaaaa tatttttaag aaatggaacc
      421 ttgattcttc tgtaatcggt aacgttatcg aaaaaaggag cttgatttta accaaagatg
      481 gcagtgtaga gtgtgatctt ccagttgata tcatcacaaa tgaagctcct tattacgaca
      541 gggaatattc taagcccgaa tatttatcac aaattaaaga cagcgttaat tataaaaaac
      601 ctgacaattt caacgatacg gttatacagc ttctatcgtc acctaacctg tgcaacaaaa
      661 actgggtaat tgagcagtat gatcatatgg ttagaacaaa cacagtctgc cctccgggct
      721 ccgatgcttc tattgtgaga ataaaaggaa cagataaatc tattgcaatg acaaccaact
      781 gcaattcaaa gtattgttat ctaacccctt atactggctc catgattgca gttgctgaaa
      841 gcgcaaggaa ccttgcctgc tcgggagctg ttcctcttgc tatcactgac tgccttaatt
      901 ttggcaatcc cgaaaatcct gagataatgt ggcagtttaa agaatcaata aacggaatat
      961 caaaagccgc tacttttctg ggtactccaa tagtaagcgg aaatgtgagt ttatataacc
     1021 agacaggaaa tgaagcaatc taccctaccc ctacaattgg tatggttgga ctcattgaag
     1081 attcatcgaa gataataaat cagtggttta aaaatgaaaa cgatttaatc atacagattg
     1141 gaaatacata cgaagaattt ggtggcagtg aatttttaaa tctccaaaac cctcacctgt
     1201 taagcagagc acccgaattg aatctagaat cagaaaaaaa acttataaat atattattgg
     1261 aattgataca agatggtata cttaattgcg cacacgatat ttcagaagga ggccttataa
     1321 cggctttagc tgaatcatgt tttacaccag aaggccaaat aggaatcaaa attgaaaatc
     1381 cgcttccaaa ctcattaact gaggatgcat ttttattctc tgagtctcag tcaagagtaa
     1441 tagtatcttt agataagaaa aaattagcaa aatttgaaaa agtattaaat aagcatgaaa
     1501 tggaatttca tctccttgga acaaccgggg gggatagctt agtgattgaa aatctaatta
     1561 atatgcctgt aaaagaagct ttcgatgcct gggacaccgg gtttattgaa ttactgaaaa
     1621 ataatggcta aaataagaga agaatgcggc gtattcggta tttttggtca tcccgaagct
     1681 tctaacatca catacttggg gctgcatgct ttgcagcata gaggtcagga aagtgcaggc
     1741 atagttacat ctgacgatgg tctgctctat gaacataagg aaatgggtct tgtttcagac
     1801 attttcacag aagaggtaat taataaacta aaaggtaata atgcaatcgg ccatgtgagg
     1861 tattcaactg caggaatgag ccaccataaa aactctcagc ctcttgtcat aaaccattcc
     1921 aaaggacagc aaattgcagt agcgcataac ggcaacctta caaatgctag aaccctgagg
     1981 gaagagcttg aatctgacgg ttctatattt cagactacta cagacacaga agtaatatta
     2041 catttcatag ctaaatccaa agaacagact ttaaagaaac gtgttattga agctcttctc
     2101 aaatgcaagg gggcctattc tttacttttc ttaacaccag aatctataat tgcctgcaga
     2161 gatccctatg gtttcagacc tctggttttg ggaaaacttg gaaattcata cattgtatca
     2221 tccgaatcct gttcgtttga tcttatagag gcagatttca taagagaaat agaacccgga
     2281 gagctattaa taattaatga agacggcata gaatcattaa aaccgtttcc tgaaacaaaa
     2341 tctaaacact gtatttttga acacatttat tttgcaaggc ctgacagcta tctatttgga
     2401 gataatgtat acaaagtaag aaaggaattt ggaagacagc ttgctataga acaacctgca
     2461 gatgctgata tcgttatagc cgtaccagat tcaggcgtac cggcagcaat aggttattcg
     2521 gaacaatcag gccttccgct cgagcttgga ctcctaagaa gtcactatat gggaagaaca
     2581 tttatcgaac caagacaggt tattagaaat tttggagtaa agcttaaact aaacgctata
     2641 agaaatgtaa tagatggtaa aaggcttgtt atagtagatg attcaatagt aagaggaact
     2701 actagcagaa aaattgtaaa gatgctccaa aacgcaggcg ccaaggaagt tcatatgcga
     2761 ataagttcac ctcctatgaa atatagctgc tattatggag tggatactcc aaacgatgga
     2821 gagcttatag caaactcact ctctgttgag gaaataaata aatacattac ttctgatagt
     2881 cttggttatt taagtctttc tggaataatt gaagctgtgg aaaggtcaaa cccgactact
     2941 tgcggctcta atttttgtaa tgcatgtttt acaggctcat atccagaaca gcttgtagac
     3001 aagactgata aactcaagca gctccatctg tttgaaagat aatatttagt tactgaattt
     3061 tactttttct cagataaccg gtcgatattt tcttttataa ctgctttttt atctgttagt
     3121 tctattaatt tacccttctc tttatccact atctcttttg gtgcacgctc aataaacttt
     3181 ttgttactta acttgttttc aattacaacc agatctttat ctatcttctc tatttctttg
     3241 ttcattcttg atatttcatt atcaatatcg actaaacctt cttcgggcac aaatatctga
     3301 acaccgctta aaacctgaga gacacagttt tgcggttttt catcttttat aaattctatt
     3361 gagtcaactt tagcaagttc gtatataaat tgtttattat gagatattag gttttctaat
     3421 tcttcacttt cagtgttaac aaatattttt attttttgtg aaggttttac atcaagctca
     3481 gccctgatgt ttctaatgga tattacaatc tgttttaaga attctacttc tttatactca
     3541 tcatcgaata tttcagattc attgtttata gggtatgaac ttactacaag gctctcagta
     3601 acattgcctt tattatcaac aatacttact ccaaagtttc ttaacatgct gaatatttct
     3661 tctgttataa aaggagtaaa aggatgcatt agctgaagag aatatactag tagttttaca
     3721 agaatgtttt gtgtattttg cctttgagta ttgtcttcag accttaattt tgattttgat
     3781 atttcaatat accagtcgca gaagtcaccc cagataaact gatataccct atttgctact
     3841 ttatcatagt caaaattgtc ataatcctgg tcagtctctt gtattatgtt attaaattta
     3901 gtaagaatcc atttatcaaa tattgataaa gaatttttgt catatgtaca atttggatca
     3961 taaccttcca aattaagcat taaaaacctt gaagcattcc atattttgtt aatgaaattt
     4021 ctatatccct tgataacaga cattgatagc ttaatgtctc ttccctgtga tgccatggca
     4081 gcaagactaa atcttagtga gtctgctcca aattcatcta taatttccag cgggtctata
     4141 acattgcccc tggttttact catcttctca cccttttcat ctcttatcag accatggata
     4201 taaacgtctc taaatggcac ctgccccgta aacttaatac ccatcatgat catccttgct
     4261 acccagaaga atatgatatc gaatgcagtt attagcacac ttgtaggata gtatttttta
     4321 agatcaacag tttgctcagg ccaccctaag gtagtaaagg gccaaagacc cgatgagaac
     4381 caagtgtcca gcacatctgt atcctgggta agttctattt ctttgccgta atgcagggtt
     4441 gctgatttta atgcatccga ttcactaagt tctacaaata ttttgccatc aggcccgtac
     4501 catacaggca cctgatgccc ccaccatatc tgccttgaaa tacaccatgg ctcaatatta
     4561 ttcatccatt caaaaaatgt attttcccag tacttaggat gaaattttat ttccccatct
     4621 tcaaaagcct tgatagcatt tttggcaagc tctttagcat ctacatacca ctggtaagta
     4681 agcctcggct caacaatctc tgctgacctg tcaccatatg gaacaaagtg ttttatatct
     4741 tcatcctttt ccaaaagccc caagtcttca agatcactta atatttgttt ccttgcctta
     4801 aatctggaaa gcccttcata tttatccggc acattggaat ttaatacggc actttcagta
     4861 aatatatcta tcatctcaag atcatgcctc tttccaactt caaaatcatt aaaatcatga
     4921 gcaggggtaa tcttgacagc gccactacct ttttccggat cagaatattc atcagccact
     4981 attggtattt ttctatccgt aagaggaagc aatacatatt tacctattaa tttttcataa
     5041 cgtttatctt gaggatgcac agcaactgcg gtatcgccaa gcatggtttc aggtcttgta
     5101 gttgcaactg ttatatattt ctttgaattt tctatgggat attttatata ccagagtttt
     5161 ccttttactt ctctctgatc aacctcaagg tctgataccg ctgtctttag ttttacatcc
     5221 cagttaacaa gccttttatc tttataaata agcccctcat tgtataaatc aacaaacacc
     5281 tttctaactg cctgggataa gctattatca agagtaaact tttccctact ccagtcgggc
     5341 agagcaccaa gtctttttaa ctgatttgta attgtacctc ctgactcttc tttccatttc
     5401 catactcttt ttacaaactc ttctcttcca agttgttctt ttagaatatt ttcttttgca
     5461 agctccctct caactatcat ctgtgtcgca ataccggcat gatctgttcc cggaacccaa
     5521 agcgcatcga aacccttcat cctcttgtat ctgatgatga tatcctgaag ggtattattg
     5581 agggcgtgac ctatatggag tgatcctgta acgttagggg gaggaattac tatggtaaac
     5641 ggtttatttg aagattcaga gtccgtacta tagtattttt tttccatcca aaggtcatac
     5701 cacttctgtt ctagactctt tggattgtaa gacttctcaa tctgactgta tagatcttca
     5761 gacattcagt tcagcaagac attaacaatt ttattcaatt aaacagtctg tgtttcttct
     5821 tcaattggta aaggaacagg aatctcagga gcttcaacct caatatcaat attcctgtac
     5881 attggaagtc ctgtacctgc cggtattagc ctacccataa ttacattttc cttaagtcct
     5941 ctaagcttgt cttccttacc ttcacaagca gcttctgtta acacccttgt tgtttcctgg
     6001 aatgacgcag cagaaagcca gctctctgtg cttaaggacg ctcttgttat accaagaaga
     6061 agtggttcag cagttgcggg tttaccgccc tgctctatta ttttctgatt ttcttcaaag
     6121 aaggtttgct tttcaatagc ttcacccaca agaaacgtag tatctcccgg ctctttaatc
     6181 ctcaccctct ttaacatctg tttaactatt gattcaatgt gtttatcatt aattttaaca
     6241 ccctgcagcc tgtaaacttc ctgtatttca tctacaagat atctcgaaag tgctttctct
     6301 cccctaatgc taagaatatc atggggatta acagagccgt caaccagctg atctccggca
     6361 gtaacccttt ctccatctct tactaatacg tgctttcctc ttggaacgag atattctttt
     6421 ggctcaccca tctcaggagt aactacgagt cttctcttac cctttaaatc ttttccatat
     6481 gtaacgattc catcaatttc tgttacaacg gcgggatctc ttggaattct tgcttcaaaa
     6541 agttcagcaa cccttggaag acctccggtg atatcctttg tctttgcagt ctctcttggt
     6601 atcttagcga taacatcacc tgcaaatatc tcttctcctg aattcttttc tataactgct
     6661 cctaccggaa gcgagtactg cacatgcccg ccttcagcat tttcaattat cattgcaggg
     6721 tgataatcag gattttttga ttccatcact acctttttat aaatacccgt tacttcatca
     6781 acctgctctt tcatacttat gccttcttct aaatcctgat actttgatac tcctgaaata
     6841 tcagaaataa atggtgtgtt atatggatcc caatccgcta ttagatcacc ctctttaact
     6901 ctatcaccct ctgagacctt aagt
//
LOCUS       DBNGSV010000232        18485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_317725, whole genome shotgun sequence.
ACCESSION   DBNGSV010000232 DBNGSV010000000
VERSION     DBNGSV010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18485
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_317725"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1233)
                     /locus_tag="AC159U_08435"
     CDS             complement(106..1233)
                     /locus_tag="AC159U_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3337125.1"
                     /translation="MNFDNTVATSDFQIIENSATTSKTTFSPSGKKSKVSVHSKGRKP
                     YISSSENPSLQRYFNEVGQEYTFNSKQEIVYAVKLRSFEAYYKQINAKINSIKKTLCN
                     ELQSQKSQHINKTNELQRLNTISLVTKNSIQHFKSQFASSNLKIVISLAKNFSNRGLP
                     YSDLIQEGNIGLLKAIDRFDPHKGYKFSTYESWWIQQSMNRAVLDQSRLIRLPVKIQE
                     EFFKISKSPKSLTKDNGTAPKTEDISVHLGINDKKVKKVEKAASLSVISLDTPKYKAN
                     SESGNTIQDFIPDNTDCVETYISQIEMSVNISKALSKLSEREQNIISMRFGIGYKHNY
                     TLDEIGNSYDLTRERIRQIERNALIKIKNNPDGDILREYLT"
     gene            1732..2661
                     /locus_tag="AC159U_08440"
     CDS             1732..2661
                     /locus_tag="AC159U_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3337126.1"
                     /translation="MKILITGGAGFIGSWVADKFIENGHDILIVDDLSSGTESNINNK
                     AQFIKCDIRNKDLLSKIFREFKPDIVDHHAAQINVRTSVDDPVYDAEINIIGSLNLLE
                     LCRDHKVSKFIFASTGGAIYGEPPHIPADEDTKPMPISPYGTSKFAVEQYINYYHQVY
                     GLQYVALRYANVYGPRQNPHGEAGVVAIFCNKILANEPCMIFGDGTQTRDYVYVSDVA
                     DANILALNAECGCYNIGTGVETDVLKLTELLGNCANGYKLEVKHADEREGEVQSISLD
                     NTLSKNKLGWIPKVNINGGIEQTWNWFLDSSKK"
     gene            2724..2957
                     /locus_tag="AC159U_08445"
     CDS             2724..2957
                     /locus_tag="AC159U_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337127.1"
                     /translation="MKDKFDIDFDVRVVDRSIRDSKITRKEYDEKLEALEDLSELAEP
                     LVIEDDTEQEQDENEDTNETAEQQLEENVEEQE"
     gene            2957..3166
                     /locus_tag="AC159U_08450"
     CDS             2957..3166
                     /locus_tag="AC159U_08450"
                     /inference="COORDINATES: protein motif:HMM:NF025156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HIR3337128.1"
                     /translation="MPWEYKVVSIESLIPDDGDHDIAVSKQDAQNRKEKLSNNMQDSL
                     NNLGNDGWELVSIFSDFGIFKKLAN"
     gene            3204..4094
                     /locus_tag="AC159U_08455"
     CDS             3204..4094
                     /locus_tag="AC159U_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337129.1"
                     /translation="MCNINFTLLTIIIVNILAGSTTYSSSLNFHRIDELNHSVSTKHT
                     NLISNSTGYSSGKTEYLVSQISENKNFYIELSYNSDHADVLTKDILSRALREMTTIFI
                     GSGYTYVKKENADYLAKVYIDAEISDYKRGDSNKYDFLSKVTIKVSDKDEQVLEQTNK
                     NVFLSGGDLHKIAVDSISKSATRAAKEIVGNLSAKKIENEDEPVVVITTSKVELVFEG
                     SVNYKLYNAIDNLIKESLSEIKVLKRTIKYGNSLNMLVLSNLRSQQLAQLVIDRIPDK
                     DSMEVINVSDNRVVFKLNQN"
     gene            complement(4104..5636)
                     /gene="guaA"
                     /locus_tag="AC159U_08460"
     CDS             complement(4104..5636)
                     /gene="guaA"
                     /locus_tag="AC159U_08460"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3337130.1"
                     /translation="MRQLVLIIDFGSQYTQLIARRVRELGIYSEIKPFNLPLEEIKGL
                     NPGAIILSGGPCSVWDKDSPRVSSDLFNLDIPILGICYGMQLTVSCLGGKVEKSKKRE
                     FGPAKLSIKHSSDLFENITNNSQVWMSHSDKVLSISKNFKIIAKTKTCPVAAIKHKDK
                     KIYGTQFHPEVVHTVEGIEILTNFLFKISGLKKNWSPKSFVQRTIEDLKNTLDDSKVI
                     CGLSGGIDSTVTAALLKETIGKNLHCIFVDTGLLRLNEAKEIVQSYKQMELNLKVVNA
                     QKVFLQKLRGVIDPEKKRKIIGREFVKVFEAEAKKIKGAKYLAQGTLYPDVIESVSVK
                     GPSATIKSHHNVGGLPKRMNLKLVEPLRELFKDEARKAGKELGVPTEILGRHPFPGPG
                     LAIRIMGEITKDRLEILRQADRLFIDELKSSNTYDDIWQAFSVFIPVRTVGVMGDERT
                     YENVLALRAVTSTDGMTASWAKIPYDILERVSVKIINEVKGINRVVYDISTKPPSTIE
                     WE"
     gene            complement(5691..7157)
                     /gene="guaB"
                     /locus_tag="AC159U_08465"
     CDS             complement(5691..7157)
                     /gene="guaB"
                     /locus_tag="AC159U_08465"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3337131.1"
                     /translation="MLEDKIEESLTFDDVLLLPSYSEVLPGDVDVCTVLTPNIKLNIP
                     LISAAMDTVTEAKTAISMASEGGIGIIHKNFTIEDQASEVEKVKKYEGGMIINPLTVR
                     PDNTVGDAVNIMLTKNITGLPVTRSDNTLLGIVTYRDLRFERNLDFPVKKIMTKKSKL
                     VTVKEDISLESAKQLLHKYKIEKLPVVNDKFKLTGLITMKDIEKIEKHPLACKDKLGR
                     LRCGAAVGVGEDSEQRVQALIDANCDVIVIDTAHGHSQRVLDAVKSTKKNHPDTELIA
                     GNIATTEAAEALIKAGADCLKIGVGPGSICTTRVIAGIGVPQITAIMNVVKAAKKHNV
                     PVIADGGIKYSGDITKAISAGANSVMIGNLFAGADEAPGEVILYQGRTYKVYRGMGSI
                     EAMKKGSKDRYSQDEMIDSKLVPEGIEGRIPNRGSIGSVIYQLLGGLRAGMGYTGCKN
                     INELREKSRFTKITNAGLKESHVHDVIITKEAPNYRVE"
     gene            complement(7161..7526)
                     /locus_tag="AC159U_08470"
     CDS             complement(7161..7526)
                     /locus_tag="AC159U_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337132.1"
                     /translation="MKTTKLKISISQDGLLPQETVDVETLEFPVIPKKLLKALGFERV
                     DTSTFVGVLPVFVNLLDKEQEIEIELGVNQPEMRKKVLEVIQFNTPEDQRIELKPETP
                     VVKPKSKRGRKPKVKKEQE"
     gene            complement(7601..9205)
                     /locus_tag="AC159U_08475"
     CDS             complement(7601..9205)
                     /locus_tag="AC159U_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3337133.1"
                     /translation="MIIQCDQCDRKFRVDDSKIKPPGSRVRCSKCGNVFFVEKNDTPK
                     VEETAPETGISDTPSPNLDNLQVDSNRKEVTDPNQIEVGISEPQKTEGSAEIEPDAST
                     EQEDKQEQVWSIDDEQQTSSLDIEQPSIDISTGTNTDLDSLPAEVKFEPGPEPEPEPE
                     LKADLNVANGLSLDTDSPQDITEPDNSSVSWASLDSEEDSKTSDDDLTPPAQSISDMD
                     NKQVDTAPITSEFPQSPPPAEPSMDYATDSATDPVQQVAEEPSLDLDIEKVQQADNQQ
                     AFQSAHGSEAPLDSDLSVDQQTMSQVQAESYGGGAQQEFDSSSEAPSIRSTLRPQKKS
                     FGSKIGSFITKIIVAACIIVILGSVGLILAINLEVIPKEKAKELRSFLVANLPIDIED
                     PLEGINITSTPGQWINSSNGPLFVVQGNVTNLTDNIVNYIQLNAQYTNNGQVLYDTKL
                     YAGNTFTGQELQTLSLNEINERLNRKNGDIDFSDPRKLAGTNYNLMPGDSIQFYSIFP
                     SKTQILGLKHNIEVIDAELILVDNSG"
     gene            complement(9478..10962)
                     /locus_tag="AC159U_08480"
     CDS             complement(9478..10962)
                     /locus_tag="AC159U_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337134.1"
                     /translation="MAFPKEQLSKLIRAQYPIVYLVSWEEERIENNLIRLKDELYGPD
                     ADFFSWSTTGGLQNGDGKVDGSGDIQKALEIAIQSNNRGIFLIKDFHHIINDPKIIRK
                     IKDVYSAFRNTSKTLFIVAPALILPYELEKEVNVVDVDLPDAQEVREIFDSTIAQIDN
                     ETIKHSFTPELINNCINALMGLAAIEIELALKRVLVDKTSADNSIIEELLEEKVQIIR
                     KSGTLEFIRSNVVLDGVGGLENIKEWLHIRHHAFSAEAKDFGLDFPNGMLIMGISGCG
                     KSLCSKAVSTAWNLPLIRLELNSVYGGAFGSPEEVFRRAIKTVEASAPCILWIDEIEA
                     GITRSGEKTGDSPSSRIFGYFLTWMQEKQKPVFITATANQIDLLPPELLRKGRFDEIF
                     FVTLPSREERKEIFRIHLTNRKKDPDVYDLDSLAKNTEGLSGSEIEQAVVAGLFESFS
                     KKKELDDNELIIAASSIVPLSITMREEISALERWAADRAVKASK"
     gene            complement(10987..12531)
                     /locus_tag="AC159U_08485"
     CDS             complement(10987..12531)
                     /locus_tag="AC159U_08485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3337135.1"
                     /translation="MSKNYVKIFDTTLRDGEQAPGCGMTTDEKLKVAYNLEKLGVDII
                     EAGFPISSEGDFQSVKLVAQEIKNCEIAGLCRANINDIDRGWEAVKHSKHPRIHTFIA
                     TSEIHLKHKLRKTQDEVLAIIEKAVKHAAAYTDNVEFSCEDATRTDIDFLCKAVKLAV
                     KCGATTINIPDTVGYTVPEEFHHIITTLLERVKGLHKVTLSVHCHNDLGLAVANTIAA
                     INAGARQAECTINGLGERAGNASLEEIVMALKVRSDKNPFETGINTEHIYPTSRIVSQ
                     VTGVNVQPNKAIVGANAFAHEAGIHQDGVLKERITYEIMNPADVGIPSNKIVLGKHSG
                     RHAFRKRIEEYGYFLSDKDFEEAFKKFKDLCDLKKYVFDEDIEALISEEFMRSSDFYK
                     LVTANFSSGTNMLPVATITLFIDGIEKTVSEHGDGPVDAAYRAIAKATGESAKLLDYN
                     VGSITGGTDAQGAVTVRIEDDGIISLGQGSNTDIVVASAKAYINALNKLRWRKEHPKK
                     ASSAGV"
     gene            complement(12600..14081)
                     /locus_tag="AC159U_08490"
     CDS             complement(12600..14081)
                     /locus_tag="AC159U_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3337136.1"
                     /translation="MKNTILINRTFNETRVAYIKNGNLFELYVERTSKPKLAGNIYKA
                     KVGKVIPGMQAAFVNYGGDKAGFISAEDVYSDSFENIFLEQQDDLEDGQLPSYLIQDV
                     LTEGQDILVQILKNPSGGKGAKLSSNIALPGKYLVLLAMVDFVGVSKKIEDTEERERL
                     IEILKEIKPENIGVIARTVSMGIGKQLLENDLAQLQQTWELIKNKSEKHNAPVQLYEE
                     PPLYIKTIRDLVTDSTEVLVDDRDYYQKITEFIENNFPNFDIKLQLYEEKTPLFTKYD
                     LEQEIQKLFEKKIWLESGGYLIIEEAEALTVVDVNTGKYLDGENQNDTIFHINKEAAI
                     ETAKQIKLRNLVGIIVIDFIDLKDTDKTNEIYNLFIDEMKDDKARTVVHEMSQFSVVQ
                     LTRQKLRESIMEYLTNDCYHCRGTGYLKSPETTTYEIFREIKEKLSRNNIDSITITTK
                     PQTIDYINNFEQENLKSLVESYGIEIKFIKDIELSKYYEITII"
     gene            14252..15292
                     /locus_tag="AC159U_08495"
     CDS             14252..15292
                     /locus_tag="AC159U_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3337137.1"
                     /translation="MKLKLILPFLLLFSAFAVAADNTGDKDAKVKSTEPQVKDNVSIY
                     VVRPGDSLWKISKNFYDTPVLWPRLWKLNPAIDKPYRIYPGEVISLKRQVPKLAVVDF
                     DPEKRDFIVGDIDPPPPVYYYSRAHNAGFISPDEWEHMGTIINSEPPRIMLGKENTLA
                     YINLGTDDNVAVGDTFTVFKSSKPVLHPVTKKRIGYKVAILGELEVHEVLGEKLSSAK
                     VTASNREMHRGARIRPTEEFVTEVVIRKGEEDLDGVIVDNKYNLAYAAQNDVVYIDAG
                     SQDNVVPGNTFTIFQHPRIVKDLDSGKNLTVPKLPIGKLVVINVKKHISTGVVIESKR
                     QVAKGDIVSLDR"
     gene            complement(15377..16336)
                     /gene="meaB"
                     /locus_tag="AC159U_08500"
     CDS             complement(15377..16336)
                     /gene="meaB"
                     /locus_tag="AC159U_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3337138.1"
                     /translation="MSLTDKDKKLIDKMLKGDRAALARLISKVENNRQSASSIIRSIN
                     HQTANAYVIGITGPPGAGKSTFVSSLTALFRKRGSKVGIIAVDPTSPFTGGAVLGDRI
                     RMQDHSVDKDVFIRSIGSRGSQGGLSKATAQIVKLYDAFGFDIVIIETVGVGQTELDI
                     IKIADTVIVILVPESGDVVQTMKAGLMEIADIFLVNKSDRQGADKICREIKSITSLSQ
                     REDKWNIPVLLTAASKNDGIKEVKDSVVKHMYFRMKHNLASEKLEKQREEELIKIVRE
                     ALDNRLFSKMQDSDIKKVVKSVREGQLDPYQAAEKIVSKLIQQ"
     gene            complement(16339..16644)
                     /locus_tag="AC159U_08505"
     CDS             complement(16339..16644)
                     /locus_tag="AC159U_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337139.1"
                     /translation="MSGKKEFSDLGEAIDHIIGTLDESEIEAVKNADPAGLHMALDRW
                     IKKDYVYSGSTNINDLVREKVKNEDMYYKDNPDAEVSVHPDNISGIIIDGLIEKLQK"
     gene            16731..16949
                     /locus_tag="AC159U_08510"
     CDS             16731..16949
                     /locus_tag="AC159U_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337140.1"
                     /translation="MKKYEYEILFHRNVKGHVTWYFASKPNKKVGTSLIKILNKLGAK
                     GWDASISGTLEAGAIAEVLLKREKGKKK"
     gene            complement(17260..17952)
                     /locus_tag="AC159U_08515"
     CDS             complement(17260..17952)
                     /locus_tag="AC159U_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337141.1"
                     /translation="MSEILDKIKRRGHWEVIIRPTIYKDNFIESTAKCKEIIRELKVS
                     LRGWEFPFYPDDKPPTTGLDFIEHPLDWSYFVEYWRYYQNGMFVHYSGLVEDWQDQVS
                     EDRRWKLPNGKSLSILSTLYKITEIFEFASRLANKGLLGNECIIYINLHDTRDRKLFM
                     LDIRRHILAEYICAIEKIDREYPLTIEELIANSSKLSLDHTIWLFQRFNWDNPPESVL
                     KEDQEKLLRGLI"
     gene            complement(17953..>18485)
                     /locus_tag="AC159U_08520"
     CDS             complement(17953..>18485)
                     /locus_tag="AC159U_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AlbA family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3337142.1"
                     /translation="KAKITKTILGMSNIRDGGNIIIGVDRNDDDSYTCTGLENNVLNT
                     YTKDNIASFVDVYSDPYVNIDVFKLPYKNKSFAVIKVFEFNEIPVLCKKDYNPNNNII
                     LKDGAVYTRSYRKPETIEVPSQSEMREILDMAVEKKLKKQLMIMENLGITQEQVSKSR
                     KDEDKRKFEEKLKDIK"
BASE COUNT         5252 a         3568 c         3347 g         6318 t
ORIGIN      
        1 aagttaaata gcttgtaata atctggttaa tttattaata aaaatgatat gagaacaaac
       61 agaaatgtag tgataataat ttaaatacca gtaaattgta tatatttaag tgagatattc
      121 ccttaatata tctccatctg ggttgttttt gatttttata agagcatttc tttcgatctg
      181 tcgtatacgt tcccttgtta gatcataaga gtttccgatt tcatccagtg tatagttgtg
      241 tttataccca attccaaatc tcatactaat gatgttctgt tctctttcgc ttagctttga
      301 aagtgccttt gatatattaa cgctcatctc aatctgcgaa atatatgttt ctacacagtc
      361 tgtgttgtcg gggataaagt cctgaatagt attcccgctt tcactattgg ctttgtactt
      421 tggagtgtct aaagaaatta cggataaact agcggctttt tcgacctttt taactttttt
      481 atcattaatt cctaaatgta cagatatatc ctcagtcttg ggtgcggtgc cgttatcttt
      541 ggtgagtgat tttggagatt tactaatttt aaagaattct tcttgtattt ttacaggcag
      601 tctgattaat ctggattgat ccaacactgc tctattcata ctttgctgaa tccaccagga
      661 ttcatatgta gaaaatttat atcctttgtg tggatcaaat ctgtcgattg cttttagtaa
      721 accaatgttt ccttcctgta taaggtcact atatggaagc cctctgttgg aaaaattctt
      781 tgcaaggcta ataactatct tgagatttga gcttgcaaac tgagatttaa aatgctgtat
      841 ggagttttta gttacaagag aaatagtatt taatctctgg agttcattgg ttttatttat
      901 atgttgagat ttttgtgatt gtaactcatt gcaaagggtt tttttaatgg agttaatttt
      961 agcgtttatc tgtttgtaat aagcttcaaa tgatcttagt tttacagcgt atactatttc
     1021 ctgttttgaa ttaaatgtat attcctgccc aacttcgtta aaatatcttt gtagtgatgg
     1081 gttttctgat gatgaaatat aaggttttct gcctttggaa tgaacagaga ccttagattt
     1141 tttgccacta ggagagaatg tggtttttga tgtcgtagca ctattttcta taatttgaaa
     1201 atcagaagta gcaactgtat tatcaaaatt catacttaat actaaagcaa tatacgtgcc
     1261 actagataat gttacattaa aaccaagcac attcttttct atagaatgat taagatttta
     1321 ttggtataca acaacttagg tattaagttt gagtatgact agtaactgct cgatcataaa
     1381 ggaagttttt aggaggcaca ttctatatgc atatacgaaa taaaccttat tgctgctatt
     1441 tctaactaat taatattact atatatttta ttctataagc ctttattgtt gacaataatt
     1501 gtatgtactt ccataaattg tcagtttcta taaagctacg attttaatat aaggtgtatc
     1561 taaattatct aatatattgg taatatcccg gttttttaat caattaataa ctcgataaat
     1621 ttcctcaagt acaatttatt tattgttttg tgacacttac cgttgtttat taccagaatt
     1681 tagataatat tagcagtctt gttagtgaca taaattattt aatataaaac tatgaaaata
     1741 ttaattacag gaggagcagg atttattggg tcctgggtcg cagataaatt tattgaaaat
     1801 ggccatgaca tacttatagt ggatgatctt tccagcggta ctgaatcaaa cattaacaat
     1861 aaagcgcaat ttattaaatg tgatataagg aataaagatt tattatcgaa gatttttagg
     1921 gaatttaaac ccgatattgt agatcatcat gcagctcaaa taaacgtgag aacgtctgtg
     1981 gatgatccgg tttatgatgc tgaaataaat ataatcggtt cattgaacct gttggaactt
     2041 tgcagagacc ataaagtcag caaatttata ttcgcttcaa caggcggtgc aatatacggt
     2101 gagccacctc acatacctgc tgatgaagat actaaaccca tgccgatttc tccctatggc
     2161 acttccaaat ttgcagttga acagtacatt aactactatc atcaggttta tgggctacaa
     2221 tacgttgcgc tcagatatgc aaatgtatat ggaccccgtc aaaatcctca tggagaagca
     2281 ggcgtagtgg ctattttctg taataaaatt cttgcaaatg aaccatgcat gatatttgga
     2341 gacggtaccc agaccaggga ttatgtatat gtaagtgatg tggctgatgc aaatatatta
     2401 gcattaaatg ccgaatgtgg atgttataat attggcacag gagttgagac tgatgtactg
     2461 aagcttactg aattgcttgg aaattgtgca aacggttata aattagaggt taaacatgca
     2521 gatgaaagag aaggggaggt gcagagcata tctttagata acactttatc taaaaataag
     2581 cttggatgga ttcccaaggt aaatattaat ggtggtattg agcaaacctg gaattggttt
     2641 ttagattcca gcaaaaaata gcatgcttat aaaaatagga tacaataatt aacagtcagt
     2701 tagatatcag gaggagtaaa ggtatgaaag ataaatttga tattgatttt gatgtaaggg
     2761 tagtggaccg tagtattaga gatagcaaga taaccagaaa ggagtacgat gagaagctcg
     2821 aagctttaga agatctaagt gaattggccg aaccgcttgt tattgaagat gatacagagc
     2881 aggaacagga tgagaatgaa gatacaaacg aaactgctga gcagcagtta gaagaaaatg
     2941 tagaggaaca agaataatgc cctgggaata caaggttgtt tctattgaaa gcttaatacc
     3001 ggatgatggt gatcatgata ttgccgtatc aaaacaggac gcacaaaaca gaaaagaaaa
     3061 actgtcaaac aatatgcagg atagtctcaa caatcttgga aatgacgggt gggaactcgt
     3121 atctatattc agtgatttcg gaatatttaa aaaactagca aactaaactt acaaactcaa
     3181 tccaaatagc tttaatatat gcaatgtgca atattaattt tacgctactt actataatta
     3241 tcgtaaatat attagcaggc agcactacat atagttcatc tcttaatttc cacagaattg
     3301 atgaattaaa ccattcggtt agtacaaaac atacaaattt aatttcaaat agcacgggtt
     3361 attcttctgg caagacggag tacctggtat cgcagatttc tgaaaataag aatttttaca
     3421 tagaactttc ctataattcc gatcacgcag atgttctcac caaggacatt ttatcccggg
     3481 cgcttagaga aatgacaact atttttattg gctcgggata tacatatgta aaaaaagaaa
     3541 atgctgatta tctggcgaag gtatacattg atgcagaaat ttctgattac aaacgtggag
     3601 attcaaataa atatgatttc ctatccaaag taacgattaa ggtctctgac aaagatgaac
     3661 aggtattgga acaaactaac aaaaatgtgt ttttatccgg aggtgattta cacaaaattg
     3721 cagttgattc aataagcaag agcgctacca gggccgcaaa agaaattgta ggaaatctaa
     3781 gcgctaagaa aatagagaat gaagatgaac cagtagtggt aattaccaca tccaaagtgg
     3841 agcttgtctt tgaaggaagc gttaactata aactttacaa tgcaatagat aatctgatta
     3901 aagaatcact gagtgaaata aaggtcttaa aacgtacgat taaatatgga aactccttaa
     3961 atatgcttgt tctatctaat ctccggagtc aacagcttgc acagcttgta attgacagga
     4021 tacccgacaa ggattctatg gaggttatta atgttagtga caaccgagta gtttttaaat
     4081 taaaccaaaa ctagttaatt catttactcc cattcaatag tgctgggcgg ctttgttgat
     4141 atatcataaa caaccctgtt tataccctta acttcattta tgattttcac tgaaacacgt
     4201 tctagtatgt cgtagggaat cttggcccag cttgcagtca taccgtccgt acttgtaacg
     4261 gctctaagtg caagcacatt ttcataggtt ctttcatcgc ccataacgcc tacggttctt
     4321 acggggataa aaactgaaaa agcctgccat atatcatcat aggtgttaga agattttaac
     4381 tcatcaataa ataaacggtc tgcctgcctc aatatctcca ggcggtcttt tgttatttcc
     4441 cccatgattc ttattgcaag cccggggccg gggaaagggt gtctgcctaa aatttcagtt
     4501 ggtacgccaa gctcttttcc tgcctttctt gcttcgtcct taaagagttc tcttaggggt
     4561 tctacgagtt tcaggttcat tctcttggga agcccgccca cgttgtgatg agacttaatt
     4621 gtggcagaag ggcccttcac acttacactt tcaattacat cgggatacag ggttccctgg
     4681 gcaagatatt ttgccccttt gatttttttg gcctctgcct caaatacttt tacaaactcc
     4741 ctgcctatga tctttctttt cttctctgga tctattactc cccgtagttt ttgtaaaaac
     4801 actttctgag cattaactac ctttaagtta agctccatct gtttatatga ctgcacaatt
     4861 tcttttgctt cgtttaacct cagaaggccg gtatcaacaa aaatgcagtg gaggtttttg
     4921 ccgattgttt cttttagcag tgctgctgtt acggttgaat caattccgcc tgaaagcccg
     4981 catataacct tagagtcatc aagtgtgttc ttcaggtctt caatagttct ttgtacaaat
     5041 gattttggtg accagttttt ctttagaccg gaaatcttga ataaaaaatt cgttaatatt
     5101 tctatgcctt ctacggtatg aaccacttca ggatgaaact gggttccata tatttttttg
     5161 tctttatgct ttattgctgc cacagggcag gttttagttt ttgcgataat cttaaaattt
     5221 ttggaaatgg aaaggacttt atcgctgtga gacatccaga cctgtgagtt gtttgtaata
     5281 ttctcaaata aatcagaact atgtttgatt gataacttag ccggcccaaa ttctctcttt
     5341 ttagattttt ctacttttcc accaaggcag cttacagtaa gctgcatacc ataacatatg
     5401 ccaaggatag gaatatccag gttaaataga tctgaagaca ccctggggct gtccttatcc
     5461 caaacactac aggggccgcc tgagagtatt attgcaccgg ggtttaatcc tttaatttct
     5521 tcaagcggta agttaaatgg ttttatttca gaatagatgc caagttcacg cactcgtctt
     5581 gcaataagct gagtgtactg agacccgaaa tctataatca atacaagttg tctcatgcaa
     5641 ttatataatt aaagctacaa atatggagat gcttaaaagt tttagaccaa ttattcgact
     5701 ctgtagtttg gagcctcttt ggtaattatt acatcatgca cgtggctttc tttaagtccg
     5761 gcatttgtta tttttgtaaa tctggatttt tctctaagct catttatgtt cttgcatccc
     5821 gtataaccca ttccagctct tagtcctcca agaagctgat aaatgacgct gccaattgat
     5881 cccctgttag gaattcttcc ttctattcct tctggaacca gtttcgaatc gatcatttca
     5941 tcctgagaat acctgtcctt gcttcccttt ttcatagcct caatcgatcc catccccctg
     6001 taaactttat atgttctgcc ctgatacaat ataacttcgc cgggggcttc atcagcgccg
     6061 gcaaacaggt ttccaatcat cacagaatta gcacctgcag aaatagcttt tgtaatgtca
     6121 cctgaatatt ttatgccacc atctgcaatc accggaacat tatgtttctt tgctgctttt
     6181 accacattca taattgctgt aatttgaggt acgccaatac cggctattac tctagttgta
     6241 catattgaac ccgggccaac accgatctta aggcagtcag cgcctgcttt aatcagagcc
     6301 tcggcagctt ctgtggttgc tatgtttcct gctattaatt cggtgtcggg atgattcttt
     6361 ttcgttgatt ttacagcatc gagcaccctt tgggagtgac catgcgcggt gtctattaca
     6421 atcacatcgc agttagcatc gataagagcc tgaactcttt gttcgctgtc ctctccaacc
     6481 cctacggctg caccgcatct tagccttcca agtttatcct tgcaggccag aggatgtttc
     6541 tctattttct ctatatcttt catggtgata agccctgtga gtttgaactt atcatttaca
     6601 acaggaagtt tttcaatctt atatttatgt agcagctgtt tggctgattc taatgaaata
     6661 tcctctttaa ctgttacaag cttggacttc ttggtcatta tttttttcac aggaaaatcc
     6721 aggtttcttt caaacctaag gtctctgtat gttactattc caagaagcgt gttgtcagac
     6781 cgggtaaccg gaagacccgt aatgtttttt gtaagcatta tattaacagc gtcgcctacg
     6841 gtgttgtctg gtcttactgt aagcgggtta attatcatgc cgccttcata tttttttact
     6901 ttttctactt cggatgcctg atcctcaata gtaaaatttt tatgtattat gccaatccca
     6961 ccctctgaag ccatggaaat tgcagtttta gcctccgtaa ctgtatccat tgcagcactg
     7021 ataagaggta tattgagttt aatattaggg gttaggacag tgcaaacatc tacgtcaccg
     7081 ggcaaaactt cagaatagga gggcagtaac agtacatcat caaaggttag agattcttca
     7141 attttatcct ctaacatgtt ttactcttgt tcttttttta ctttaggttt tctgcccctt
     7201 tttgattttg gtttaactac cggggtttca ggtttaagct caattctttg atcttcgggg
     7261 gtattaaact gtataacctc taaaaccttt ttgcgcattt ccggctggtt tacgccaagc
     7321 tcaatctcta tttcctgttc tttatctaaa aggtttacaa acaccggaag cacgcctaca
     7381 aatgtgcttg tatcaacacg ttcaaaacca agcgctttta gaagtttttt aggtattaca
     7441 ggaaactcca gtgtttctac atctacagtt tcttgaggaa gcaggccatc ctgacttata
     7501 ctaattttta gtttagttgt tttcatcgta ttttatctat tatattaaaa tgagagcgta
     7561 ttttacttca gagtttataa atagcaatgg gcaaatttta ttatcctgaa ttgtctacta
     7621 atattaactc tgcatctatt acttctatat tatgtttgag ccccagtatt tgagtctttg
     7681 aaggaaatat ggaataaaat tgtatactat ccccgggcat caaattatag ttcgttcctg
     7741 caagttttct tggatcacta aagtctatat caccgttctt tctgtttaat ctttcattta
     7801 tttcatttag agataaggtt tgaagttcct gtccagtaaa agtatttcct gcatacagtt
     7861 ttgtatcata caggacctga ccgttattag tatactgcgc atttaactga atgtagttta
     7921 ctatattgtc agtgaggttg gtaacatttc cttgtacaac aaacaaagga ccattgctcg
     7981 agtttatcca ctgaccagga gtacttgtaa tgtttattcc ctcaagtggg tcctctatat
     8041 ctatgggaag attggcaacc aaaaaacttc ttagttcttt tgctttttct ttaggaataa
     8101 cttcaagatt aatagccagg attagtccaa cactacctaa aataactatt atacaggcag
     8161 ctacaataat cttcgttata aatgagccga tcttggatcc gaaactcttt ttttgaggtc
     8221 ttaaagtaga tcttatgctt ggagcctcag aacttgaatc aaactcctgc tgggcaccac
     8281 cgccatagga ttctgcctgt acctgagaca tagtttgctg atcaacggac agatctgaat
     8341 ccaatggagc ttcagagcca tgcgctgact gaaatgcttg ctgattatct gcttgctgga
     8401 ccttttcaat atccagatca agacttggtt cttcggccac ctgttgtacg ggatctgtcg
     8461 cgctgtctgt agcatagtcc attgatggct cggctggtgg aggagattga ggaaattctg
     8521 atgtaatagg agctgtatct acctgtttgt tatccatatc agatatggac tgtgccgggg
     8581 gagtaagatc gtcatctgaa gtcttactat cttcttctga gtccaatgaa gcccaggata
     8641 ctgaagaatt gtcaggctct gtaatatcct gcggactatc tgtatctaaa ctaagtccat
     8701 tagctacgtt caaatctgct ttaagttctg gctctggttc aggctctggt ccaggttcaa
     8761 attttacttc ggccggtagt gaatcaagat ctgtattagt acccgtacta atatcaattg
     8821 atggttgttc tatatccaga ctggatgttt gctgctcatc atctattgac cagacttgtt
     8881 cttgtttatc ttcttgctct gtgcttgcat caggctcaat ttcagcagaa ccttccgttt
     8941 tttgtggttc gctgatgcct acttctattt gattagggtc tgtaacctct ttcctattag
     9001 aatcaacctg taaattgtca agatttggag aaggagtatc ggaaattcct gtttccggtg
     9061 cggtttcttc aactttgggt gtatcattct tttctacaaa aaatacattg ccacattttg
     9121 aacatctaac cctgcttcct ggtggcttga tcttgctgtc atccacccta aattttctat
     9181 cgcactgatc acattgaatt atcatatatt tatgctgttt ttctgatcaa ataatcaaaa
     9241 atagtattga tatttataac ttttgtatta aaaactaatg ctaacttaat gttaagtcaa
     9301 ctattataca atatattttg agatttttaa tagaatcggg ggaaaatact gattttttta
     9361 gattaaatta attatatttt aaaattattg ctataaaaat taagagaaaa tgggttaagg
     9421 tgtgtttaag attgctaaat tggtacataa aaatatttaa tactaaatac ggataaacta
     9481 tttagaggct ttcacagccc tatctgctgc ccagcgctcc agtgcggata tctcttctct
     9541 catggtaatt gacagaggaa caatagaaga tgctgctata atgagttcat tatcatccag
     9601 ctcttttttc ttggaaaacg attcgaacag tcctgctact acggcctgct ctatttccga
     9661 accactaagt ccctctgtgt tttttgcaag cgaatccaga tcgtaaacat cagggtcttt
     9721 tttcctgttt gttaaatgaa tcctgaaaat ctcctttctt tcttccctcg atggaagtgt
     9781 tacaaagaat atttcatcaa atcttccttt tctcagtagc tccgggggaa gtaggtcaat
     9841 ctggtttgca gtagcagtaa taaacacggg cttttgtttt tcctgcatcc aggtaagaaa
     9901 atatccgaat attctagatg atggactgtc gcctgttttc tctcccgatc ttgtaatgcc
     9961 cgcttctatt tcgtctatcc agagtataca gggagcactt gcttcaactg tttttatggc
    10021 tcttctgaat acttcctcgg gtgatccgaa tgcgccgcca tatacagagt ttagttcgag
    10081 tcttataaga ggcaggttcc aggcggttga aactgcttta gagcacaggc tcttcccgca
    10141 gccgcttata cccataatga gcatgccatt tggaaaatcg agcccgaaat cctttgcttc
    10201 agcagaaaat gcatgatgcc ttatatgaag ccattcttta atattttcca gtcctcccac
    10261 gccatctagt acaacatttg atcttataaa ttccaaagtg cccgattttc tgattatctg
    10321 aaccttttct tccaaaagct cttcaatgat tgaattgtct gcgcttgtct tatccaccag
    10381 tactcttttt aaagcaagct caatctctat tgctgcaaga cccataagag catttatgca
    10441 gttgtttatt agttctggcg taaaggagtg tttaatcgtt tcattatcaa tctgagcgat
    10501 agtactatcg aatatctcac gcacttcctg agcatcgggc aggtcaacat caactacatt
    10561 cacttctttt tcaagctcgt agggtaatat cagagccgga gcaactataa acaaggtttt
    10621 actggtattt ctaaaagctg aatacacatc ttttatcttt cttattatct ttggatcgtt
    10681 aatgatgtga tgaaaatctt ttataaggaa tatgcctcta ttattgcttt gtatcgcaat
    10741 ttccagggct ttttgtatat caccggagcc atcaaccttg ccatcgccgt tttgaagccc
    10801 cccggttgta gaccagctga aaaaatctgc atcgggcccg tagagttcgt cttttaaccg
    10861 gattagatta ttttcaatac gctcttcttc ccacgacact aaatatacta tgggatactg
    10921 agcccttatg agttttgata attgttcttt tggaaacgcc ataattgttt tctagtagta
    10981 aatgaattaa acccctgccg aggaagcttt tttgggatgc tcttttctcc accttagttt
    11041 attaagcgca ttgatgtatg ctttagcgct tgcaacgact atatccgtat ttgatccctg
    11101 cccaagggaa atgatgccat catcttctat tcttacagta actgcgcctt gagcatctgt
    11161 gccgccagtg atcgaaccta cattataatc gagtaatttt gcagactctc ctgtggcttt
    11221 tgcaattgcc ctgtaggcag cgtctacggg tccgtcacca tgttctgaaa ccgttttttc
    11281 aataccatca ataaacaggg tgatagttgc aacaggaagc atattggtgc cgcttgaaaa
    11341 attagctgta acaagtttat aaaaatccga tgatctcata aactcttcac ttataagcgc
    11401 ttcaatatct tcatcaaaca catatttttt aagatcacag agatccttaa actttttaaa
    11461 tgcttcttca aagtctttat cgcttaaaaa atatccatat tcttcaatgc gctttctaaa
    11521 agcatgtctt ccggagtgtt tgcctagcac tattttatta gatggtatcc caacatctgc
    11581 agggttcatg atttcataag taatccgctc ttttaatacg ccgtcctgat gaatacccgc
    11641 ttcatgggca aaggcgtttg ctccgacaat ggccttgttg ggctgtacat ttactcctgt
    11701 aacctgagaa actattctgc ttgtggggta aatatgctca gtattaattc cagtttcaaa
    11761 tgggttctta tcgctcctta ccttaagagc catcactatt tcttcaagtg atgcattgcc
    11821 cgctctctcg ccaagtccgt ttatggtgca ttctgcctgc ctggctccgg cgttaattgc
    11881 tgctatggta tttgctacag caagcccaag gtcattgtgg cagtgaacgc taagggttac
    11941 cttgtgaaga ccctttactc tttcaagaag tgtagtgatt atatgatgaa attcttcagg
    12001 aacggtgtat ccaacagtat ctggaatgtt tatggtggtt gccccgcatt taacggcaag
    12061 cttcacggct ttgcaaagaa aatcaatatc tgttcttgtg gcatcttcac atgaaaattc
    12121 tacattgtcc gtataagcgg ccgcatgttt tacagccttt tctataattg ctaaaacttc
    12181 atcctgtgtc tttctaagtt tatgctttaa atgaatttca gatgttgcta taaatgtatg
    12241 tatccttgga tgtttggaat gttttacagc ttcccaccct ctgtctatat catttatatt
    12301 agctctgcaa agccctgcaa tctcacagtt cttaatctct tgagcaacga gctttactga
    12361 ctggaaatca ccctctgatg aaatgggaaa tccggcttca atgatatcaa ctccgagttt
    12421 ttccaggtta tatgcaactt ttaatttttc atcagtagtc ataccgcacc cgggggcctg
    12481 ttcgccgtcc ctcagtgtag tatcaaatat ttttacataa tttttactca taacgattca
    12541 ctctgtgtaa taataaaatt gatttactat tattttacta aagataataa aattaacaat
    12601 tagatgatag taatttcata gtatttggat aattcaatat ctttaataaa tttaatttca
    12661 atcccataac tttcaacaag ggatttaaga ttttcctgtt caaagttgtt aatgtagtca
    12721 attgtctgcg gtttggttgt tatggtaata ctgtctatat tatttctgct aagcttttct
    12781 tttatctctc tgaatatttc gtatgttgtg gtttctggag atttaaggta accggtgccc
    12841 ctgcagtggt agcagtcgtt tgtaagatac tccataatgc tttctcttaa cttttgcctt
    12901 gtaagctgta caacagagaa ctgagacatc tcgtgaacaa cagttcttgc cttatcatct
    12961 ttcatctcat ctatgaacag attatatatc tcatttgtct tatcagtgtc cttaagatca
    13021 ataaaatcaa ttactattat ccccacaaga tttctaagct tgatttgctt tgcggtttct
    13081 atagctgcct ctttattaat atgaaagatg gtatcgtttt gattttctcc atccagatat
    13141 ttgcccgtgt ttacatctac tacggtaagc gcttcggctt cttcgattat taaataacca
    13201 cccgactcaa gccagatttt cttttcaaaa agtttttgga tctcctgttc gagatcatat
    13261 ttggtaaaaa gcggtgtttt ttcctcataa agctgcagct taatatcaaa attgggaaaa
    13321 ttgttttcaa taaattctgt gatcttttga taatagtccc tgtcatctac aagtacctcg
    13381 gtgctgtccg ttacaaggtc tcttatagtc ttgatgtaga gtggaggttc ttcatacagc
    13441 tgtacgggcg cattgtgctt ttcggatttg tttttgataa gctcccaggt ctgctgtagc
    13501 tgagcaaggt cgttttcaag cagttgtttg cctatcccca tgcttacggt ccttgcaata
    13561 acaccaatat tttcgggttt aatctctttt aatatttcaa taagtctttc acgttcttcg
    13621 gtatcttcaa tcttttttga aactccaaca aaatcgacca tggcaagaag tacgaggtac
    13681 ttgccgggaa gggcaatgtt ggaagatagc tttgcgcctt ttcctcccga cgggttttta
    13741 agtatttgta ccaatatatc ctgaccttca gttaaaacgt cctgaataag ataagaaggc
    13801 agctgtccat cttccagatc atcctgttgt tctaggaaaa tgttctcaaa tgaatcactg
    13861 tatacatcct ctgcagaaat aaaccctgcc ttatcgcccc catagttaac aaacgcagcc
    13921 tgcattccgg ggattacttt tcctaccttt gccttataga tatttcccgc aagtttgggc
    13981 ttggatgttc tttctacata gagctcaaaa aggtttccat tcttgatata tgcaaccctt
    14041 gtttcgttaa aagttctgtt aattaatatc gtgtttttca ttattttagg actatgaatt
    14101 aaattaaaca actattatag actagatatt atttttaaaa agataaaaaa aattaatata
    14161 tggtctttca attatgttaa aaacatgtta acttatatta agtttgttca ataagtcact
    14221 gtattgatga tatttttttg gaggtttggt gatgaaatta aaattaattt taccattttt
    14281 attattattt tcagcctttg ctgtagcggc tgataacact ggtgataaag atgcaaaggt
    14341 aaaaagtaca gaaccgcagg taaaggataa tgtatcaatc tatgttgttc gtcccgggga
    14401 ttctctttgg aagatttcaa aaaacttcta tgatacgcct gttctatggc caagattgtg
    14461 gaaattaaat cctgcgattg ataaaccata caggatatat cccggtgagg taatttctct
    14521 aaaacgtcag gtaccaaaac ttgccgtagt agattttgat cctgagaaaa gagattttat
    14581 agttggagac attgatcctc cacccccagt atactattac tcaagagctc ataacgctgg
    14641 ttttataagt cctgatgaat gggagcatat gggtactatt atcaattcag aacctccaag
    14701 gatcatgctt gggaaggaaa acactctggc ttatataaac ctgggtacgg atgataatgt
    14761 ggcagtaggt gatacgttta cggtgtttaa aagttcaaaa cctgtactgc atcctgtaac
    14821 gaaaaagaga ataggatata aagtggcaat attgggtgag cttgaggttc atgaagtgct
    14881 tggtgaaaaa ctttcatctg caaaggttac agcttcaaac agggagatgc acaggggagc
    14941 caggataaga cctaccgaag agtttgtaac agaagtagta atcagaaagg gtgaagaaga
    15001 tttggacgga gtgattgtgg ataacaaata caaccttgca tatgcggctc agaatgatgt
    15061 tgtgtatatc gatgcaggaa gccaggataa tgtagtacct ggaaacactt ttacaatctt
    15121 tcagcatcca aggatagtaa aagatctgga ctccggcaaa aacctcactg ttccaaagct
    15181 tcctattgga aagcttgtag tgataaacgt taaaaaacat atttctacag gtgttgtaat
    15241 agagagtaaa aggcaggttg ccaagggcga tatagtaagc ctagacagat aaagacagat
    15301 aaagaaaaat aaaaattaat tatagattat aagccctgct ggaagttgct ttcggcgggg
    15361 cttttttttg tttaggtcat tgttgtataa gttttgaaac aatcttttct gcagcctgat
    15421 aaggatcaag ctgtccttct cttacgcttt ttactacttt tttaatgtcc gagtcctgca
    15481 tttttgaaaa aagcctgttg tccagcgctt ctctgactat ttttataagc tcctcttcac
    15541 gttgtttttc aagcttttct gaggcgaggt tgtgtttcat cctgaaatac atgtgtttta
    15601 caaccgagtc cttcacttct tttatgccgt cgttcttgct ggcagcagtt agcagcacag
    15661 gtatgttcca cttgtcttct ctttgactaa gagaagttat gctctttatc tccctgcata
    15721 tcttatcagc accctgcctg tccgacttgt ttactaggaa tatatcggct atctccataa
    15781 gcccggcttt cattgtctgc actacgtctc ccgattcagg cacaaggatt acgatcaccg
    15841 tgtctgcgat ctttattata tcaagctctg tctgccctac gcccacagtt tcaatgatca
    15901 ctatatcaaa gccaaatgca tcatacagtt ttactatctg tgccgttgcc tttgacagcc
    15961 cgccctgact tcccctgctt cctatgcttc ttataaatac gtctttgtct acactatggt
    16021 cctgcatcct tatcctgtcg ccaagcacgg cacctccggt aaaggggctt gtgggatcga
    16081 ccgctattat tccaaccttg ctgcccctct ttctgaaaag cgccgtaagc gagcttacaa
    16141 atgtactctt tcccgctccg ggagggccag tgataccaat aacatatgcg tttgcggttt
    16201 ggtggttaat agaccttatg atggacgatg cgctctgtct gttgttttcc accttgctta
    16261 taagccttgc aagcgccgcc cggtctccct taagcatttt gtcgattaat tttttgtctt
    16321 tatctgtaag gctcatgatt atttttgcag cttttctatt aatccatcta ttataattcc
    16381 cgaaatatta tcagggtgta cgctaacttc agcatccggg ttatctttgt aatacatatc
    16441 ttcgttcttt actttttcgc gcacaagatc atttatgttt gtagagccgc tgtatacata
    16501 gtcttttttg atccacctgt ccagtgccat atgtagccct gcggggtctg catttttcac
    16561 agcctctatc tcgctctcat caagcgtgcc tataatatga tcaattgctt cacccaaatc
    16621 gctaaattct tttttcccgc tcatatcagg ataaaaataa tatatcaata taatagaatt
    16681 tataggataa ataatctatt atttaattat aagctctaaa ggagataaat atgaagaaat
    16741 acgaatacga aattttgttt catagaaatg ttaaaggaca cgtcacatgg tattttgcct
    16801 ccaagccgaa caagaaagtg ggcacaagcc ttataaagat acttaacaaa ctgggcgcaa
    16861 agggctggga tgccagcata tcgggcacac ttgaggccgg agctattgca gaggtgcttc
    16921 ttaaaagaga gaaggggaag aagaagtaga gtttatcctt tttttatatg tcatctcgaa
    16981 ccgaaggtag agatctgctt tttcctaaca cactgagcta gtcgaagtgt gagaacagca
    17041 attacttccc accactgtca ttgcgaagtt cacgaagtga actgtggcaa tctcgctttt
    17101 ataatgtaaa catatccctc tgaataaata agtctgttta ggcacaggcc aaatgagaac
    17161 tacccttcac ttagaataaa gtcagaataa accccttaga cccataaacc caaaaacccc
    17221 gggttaaacc ctataccttt gggtttttgg ggttaagtat taaattaatc ccctcaatag
    17281 tttctcctga tcttctttta atacactctc tggtggattg tcccaattaa atctttgaaa
    17341 tagccatatt gtatgatcaa gtgatagttt agatgagttc gcaatcagtt cttcaatagt
    17401 cagagggtat tcacgatcta ttttctcaat tgcacagata tactctgcta atatatgacg
    17461 tctaatatct aacatgaata atttcctatc tctcgtatcg tgtaaattta tatatattat
    17521 gcactcattt cctaataatc ctttgttggc taacctagat gcaaattcaa atatctctgt
    17581 aattttatac aatgtggata gtatcgataa tgatttgcca tttggtaatt tccatcttct
    17641 gtcttccgat acctgatctt gccagtcttc aactagacca gaataatgaa cgaacattcc
    17701 attttggtaa taacgccaat attctacaaa gtacgaccaa tctaatggat gttctataaa
    17761 atctaatcca gttgtaggag gcttatcatc agggtaaaag ggaaattccc aaccacgaag
    17821 gcttactttt aattccctaa taatttcctt acatttagca gtggattcaa tgaaattgtc
    17881 tttatatatt gtaggcctaa ttataacttc ccaatgaccg cgtcttttaa ttttatctaa
    17941 aatttcgctc atctatttaa tatccttcag cttttcttca aatttacgct tgtcctcatc
    18001 ctttctagac ttagatactt gttcttgtgt aatgcctaga ttctccatga tcattaattg
    18061 tttttttaat tttttttcta cggccatatc caatatttca cgcatctcag attgacttgg
    18121 gacttctatt gtttcaggtt ttcgatagga cctagtatat acagctccat ccttcaaaat
    18181 aatgttgttg ttcggattat aatccttttt gcataacaca ggaatttcat taaattcgaa
    18241 taccttaata acagcaaaac tcttattttt atatggaagc ttaaatacat caatatttac
    18301 atagggatca gaataaacat ctacaaaact tgcaatatta tcttttgtat atgtatttaa
    18361 aacattgttt tcgagacctg tacatgtata actatcatca tcattacgat ccactccaat
    18421 tattatatta cctccatcgc gaatgttgct cattcctaag attgttttag ttattttcgc
    18481 tttga
//
LOCUS       DBNGSV010000233         7494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_942227, whole genome shotgun sequence.
ACCESSION   DBNGSV010000233 DBNGSV010000000
VERSION     DBNGSV010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_942227"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC159U_08525"
     CDS             complement(<1..247)
                     /locus_tag="AC159U_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3337143.1"
                     /translation="MYILGISCFFHDSAAAILKDGKLIAAAEEERFSRIKHDFEFPDN
                     AIDFCLEFAGIDAEQLDYVVFFEKPFVKFERLLLSTLQ"
     gene            complement(432..953)
                     /gene="nusG"
                     /locus_tag="AC159U_08530"
     CDS             complement(432..953)
                     /gene="nusG"
                     /locus_tag="AC159U_08530"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3337144.1"
                     /translation="MNLPDININHKHKCWYAVYTIVRHEKAVNSSLINKNIDTFLPIR
                     EVVNRWKDRNKKVMLPLFPGYIFVNLDIGKKMDVLTTKGVVRILGVNGNPTPIPDQQI
                     NSIKGLLESSLNYDPYQFLVEGKQVKVINGPLAGAHGKVIERRGQYRLILSVDLIQRA
                     VSVEIDIDDVDVI"
     gene            complement(928..2799)
                     /locus_tag="AC159U_08535"
     CDS             complement(928..2799)
                     /locus_tag="AC159U_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3337145.1"
                     /translation="MISHSKLYTNNSYMIEIEKKDYESVLSEIISTRDFRQLIEKCIS
                     NRHHYRSIVFDISRGSKSRNYKIIKEVSKSKKISDSFIIDQAKSVVSYFTSGKEDKFE
                     DYYKTLQCPQGADEDQIRTSWIRLMKLYHPDKIGESGLEKSKKINEAYNILIDPDKRE
                     QYDRNYYPYIPIKIKNMDYKQFNNRSFIAVIASVLLIVPVFYISKNIINTDNGLQQDI
                     NIGKEKVKKESQITAKLFAPVQDGSVKQADKLEHNEEYKDTNDKGPANKRILSESADK
                     SEEMQIGVEEDLEPKHVKLGNESDNYKIDADESLEKIAQYKYIEPSIGEDIAMVEIGE
                     DLERLPPSHKEKKSIEDTSSSESINEKQGFKFPINYKVQKGDSLWSLSNKFNISVQDI
                     KRQNSINRNKIKPGDILVLEKSKDGMSENNIVVKELPIEGKEKKEITNFQQKKQTKNS
                     DQNKPKPWISGKTLERTNKTNDLSEKELLELASIGNNNSKFPDKSSVYTAISDYIFAY
                     KNRDIKKLGALFDPKAVENGVSMNKVFKMYKKNFEILDIVAYDIKFNQVKLNNNNATV
                     DGDFIISYNNIKEDLFKKSYGDINWKLTWNEKNWLINEIKYNVRSTKVGDESSRYKH"
     gene            complement(2771..3832)
                     /locus_tag="AC159U_08540"
     CDS             complement(2771..3832)
                     /locus_tag="AC159U_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337146.1"
                     /translation="MKLRLTNANYHFYSYDFENSLKDYTDTIELNPVLRQAWLDITEP
                     LLNENRSEDALLIIKYIKSITPYSNDLHWDLGLQLVRINEQELAVNTLVEVAQNNYWK
                     RAHVFILFDKLDVNKKYIIERLGSFELLKAYLEHLISKRYTTDALYLWENMANKHDDL
                     DENLKDRFVEFLISQNKYKYASDIWHKGNKELHGTNLIWNGDFEYDIENKGFDWRLTN
                     TREANIEIDRIERYSGENSLKIEFNGEKNINFHHVKKYIPVEPETEYLLSYAQKNSDI
                     TTKSGVYVEIMCGNSRVTTKTEDVSGSSPWIKKEKLFATESGCEGVMIQLKRDPVRKL
                     NNKISGTVWFDDISLEAIH"
     gene            complement(3945..6275)
                     /locus_tag="AC159U_08545"
     CDS             complement(3945..6275)
                     /locus_tag="AC159U_08545"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR3337147.1"
                     /translation="MTEYNNRYPVKANGREIREWDEYYPSEYHYNNYGNENNEDDDIN
                     VKEYLDIIIRRKWLILACIIISLVIAIVKTLTTVPLYRASATIEIKPDIPKITSFEDL
                     ARAERTNTYDYYQTQFSLIKSKTLGYLVLERLNEKISNRNQQKSTKSHKEDKISGLIT
                     SVKSIIYGKDQIKKNKSANKEAKSSKPKKKITDSQVFGFLSGISVGPVGSTRLARVSY
                     VSSDPQYAALAADTVIDVYIEWLLERRHGTSKSAREFLRTQLDQVKTKIELAEEELSA
                     FAKTVDIISLDKDIDLNYKQLVEITDALSELETEKLKKQAIIKDVTDGNYAYLPEVIE
                     NPRVQELSSSYSQLKAEYDNLSAVYGLNFPDIQKIRSQINSVQGQLKTTLSEIADSIK
                     RDYQSTIKKEEILKAKVEEKKTRSSELNEQAIQYRILEREVDTNKSIYNELLTKLKET
                     EVTSAIKETNVQVVDYSTVPRSPFKPDMKGNLIYSIMIGLMIGCFISFGIEFFSTKLR
                     DEEELKKKFPHVPYLGAVPMAEEADVDKLERIVYTNPRSVVSEAFRVIRTSILYSSAD
                     HPPKALLVTSPQPIEGKTTSASNLALSMIQSSLNVILIDADLRKPRLHKLYSSNGNSM
                     GLSTYLVGKGEVENVITKTDIDGLDFISSGPIPPNPAELLGSKKMKELIIALQEKYEH
                     VIIDGAPIAAFADSRLLSRLVDGVLLVTSVGITQKNVLKTSIEEIRKVRGKIIGTIVN
                     RLDSSRDKYKYKYYYYYSDEKRIDSKEARSISPHSD"
     gene            complement(6293..7339)
                     /locus_tag="AC159U_08550"
     CDS             complement(6293..7339)
                     /locus_tag="AC159U_08550"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR3337148.1"
                     /translation="MMNKEMVMRICRILFVSLLVLSTALVVYSCGGGGAVQNTKIVTE
                     ADIQTKQENDALNTKLLMSSGSGGVSSPADYIIGPADLLEVQVFESEKLTDKVRVSSR
                     GDITLPLLGNISVGGLSAREAELKIENQLIKEGFIKNPHVTVFIEEYNSKLVTVVGLV
                     EEPGNHSITGRQTLLDVLVAAKGFQEKAGRTVYISRSDVNGNKQTYLVDLDELLLNGN
                     SDINVVMKPGDVVYVPPAGNVYIEGAVQRTGAYPIKKDLTTISQIITVAGGVAPYGDA
                     SNIKLIRFLGNGNREIRPVDLTKIQSGEAVDPLVKDRDALIVGFSTSKRILYGLRVGL
                     GFGLFNFGYDPPED"
BASE COUNT         2167 a         1468 c         1044 g         2815 t
ORIGIN      
        1 tttgcaaggt tgaaagaaga agtctttcaa acttaacgaa tggtttttca aaaaacacga
       61 cataatccag ctgctcagca tctatacctg caaattcgag gcaaaaatct attgcattgt
      121 ccggaaactc gaaatcatgc ttgattcttg aaaatctctc ttcttcagca gctgcaatca
      181 acttaccgtc ttttagaatt gcagcagcag aatcatggaa gaaacaggaa attcctaaga
      241 tgtacattta atatactttt aaggttaatt ggttataaat tgaaatatta atttcaatat
      301 ttatttttta ttaaattcac ttcattgaga attattaatc aaagatattt ctagataata
      361 aaaatattag attatttaga tacagctccg ctacctcact tacaaagtga ataagatcca
      421 atattgttga tttagatcac atcaacgtcg tctatatcaa tttccactga aactgctcgt
      481 tgtataagat caaccgaaag tatcagtctg tattgacctc ttctttcaat cacctttccg
      541 tgggcccctg caagaggtcc attaataact tttacttgct ttccttcaac taaaaactga
      601 tagggatcat agtttaaact actttctaat aggcctttta tagaattaat ttgctgatca
      661 ggaatcggtg ttggattacc attaactcct agtatcctta ctactccttt tgtagtaaga
      721 acatccatct ttttgccaat atcgagattt acaaatatat aacctggaaa aagcggaagc
      781 atcacctttt tatttctatc cttccaccta ttaacaactt ctctaattgg taaaaacgtg
      841 tcgatatttt tatttattaa agatgaatta actgccttct cgtgcctcac aattgtataa
      901 acagcatacc agcatttatg tttatgatta atgtttatat ctggaagatt catcccccac
      961 cttcgtactt cttacattat attttatttc attaattaac caattttttt cattccatgt
     1021 cagcttccaa ttaatatcac catatgattt tttaaataaa tcttctttaa tattattgta
     1081 agagataata aaatctccat caactgtagc attattatta tttaatttaa cttgattaaa
     1141 ttttatatca tatgcaacta tatccagaat ctcaaagttt tttttataca ttttaaagac
     1201 cttattcatc gataccccat tttctacagc tttaggatca aatagagcac ctaacttctt
     1261 gatatccctg tttttatatg caaatatata gtcggaaatt gcagtgtata cagaagattt
     1321 atccgggaat ttactgttat tatttccaat ggaggccaat tctaataatt ctttttcact
     1381 taaatcgttg gtcttattag ttctctctaa tgtttttcct gatatccagg gctttggttt
     1441 attttggtcg ctgttttttg tttgcttctt ttgttgaaaa ttcgtaattt cttttttctc
     1501 tttaccctca atgggtaatt cttttacaac aatattattc tcagacatcc catccttact
     1561 cttttctaat accaaaatat ctccgggttt aattttattt ctatttatag agttttgcct
     1621 ctttatatcc tgaaccgaaa tattaaattt attagacaat gaccacaagc tgtcaccttt
     1681 ttgcacttta taattaattg gaaatttaaa tccctgtttc tcattaatag attcagaact
     1741 cgaagtatct tcaatagatt ttttttcttt atgactgggt ggtaaccgtt caagatcttc
     1801 gcctatctca accattgcaa tatcttcacc tattgacggt tctatgtact tatactgggc
     1861 tattttttct aaagattcat cagcatcaat cttataattg tcagattcat ttcccaattt
     1921 tacatgtttt ggttctaaat cttcttctac tccaatttgc atctcctcgc tcttgtctgc
     1981 tgattcactt aatatccgct tatttgcagg gcccttatca tttgtgtctt tatattcttc
     2041 attatgttct aatttatcag cttgctttac tgatccgtct tgaacaggag caaataactt
     2101 tgcagtaatc tgtgattcct ttttcacttt ttctttgcca atattaatat cttgctgtaa
     2161 accgttatct gtattgataa tatttttgga aatataaaac actgggacta ttaatagaac
     2221 agatgctata acagctataa aacttctatt attgaactgc ttataatcca tatttttaat
     2281 ttttattgga atatatggat aataatttct gtcatattgt tctcttttat cagggtctat
     2341 aagaatatta tatgcttcat taattttttt agatttttct aatcctgatt cccctatttt
     2401 atcaggatga tacagtttca ttaagcgtat ccagctagtt ctaatctgat cttcatctgc
     2461 accttgcggg cactgaagcg tcttatagta gtcctcaaat ttatcctctt tgccggatgt
     2521 aaaataagaa accaccgatt ttgcctgatc aattataaaa ctatcgctta tttttttgct
     2581 tttcgatact tctttaatta ttttataatt tcttgatttt gaaccccttg aaatgtcaaa
     2641 aacaatagag cggtagtgat gtctattgga tatacatttt tctataagct gtctaaaatc
     2701 tcttgttgag attatctcag ataagactga ttcatagtct tttttctcaa tttcaatcat
     2761 ataactgtta ttagtgtata gcttcgagtg agatatcatc aaaccaaaca gtaccgctta
     2821 tcttattatt tagttttctt acaggatctc ttttcaattg tatcatcact ccttcacaac
     2881 cactctcagt agcaaataac ttctctttct taatccaggg actacttcca ctcacatctt
     2941 cagtttttgt tgtaactctg gaattcccac acataatctc tacatatact ccactttttg
     3001 tagtaatatc ggaatttttt tgggcatagg aaagtaaata ttcagtttcg ggctctactg
     3061 gtatatattt tttaacatga tgaaaattta tattcttctc cccattgaac tcaattttta
     3121 aagaattttc acctgaatat ctttcaattc tatctatttc aatatttgct tctcttgtat
     3181 ttgttaatct ccagtcaaac cctttatttt ctatatcata ttcaaagtca ccattccaga
     3241 taagattagt accatgtaac tctttattac ctttatgcca aatatctgag gcatatttat
     3301 atttattttg agaaataaga aattctacaa atctatcttt taaattctca tccaaatcgt
     3361 catgtttatt agccatattt tcccaaagat ataatgcgtc agttgtatat cgttttgata
     3421 ttaagtgttc cagataggcc tttaataact caaaactacc caatctctct ataatatatt
     3481 ttttattaac atcgagttta tcaaacaaaa taaatacatg cgctcttttc caataattgt
     3541 tttgtgcaac ttcaactaaa gtatttacgg ccagttcctg ctcatttatt cttacaagct
     3601 gtaggcctaa atcccaatgc agatcatttg aatatggagt aatagatttt atatacttta
     3661 taattaacag tgcatcttct gatctatttt cattaagtag cggctcggtt atatcaagcc
     3721 aggcctgtct tagtacaggg tttaattcta tggtatctgt atagtccttt aaggaatttt
     3781 caaaatcata tgaataaaaa tgataattag catttgtcag cctaagtttc aacaaagcat
     3841 tagagttctg aatgcttaat cgtcttttta gatttgatac actgctgcct ttgtaaatat
     3901 tcagattgta cacattataa gcagtatata agactattaa gaaactagtc gctatgagga
     3961 ctaatactcc ttgcctcttt actatcaatt ctcttctcat cagaataata gtagtaatat
     4021 ttatatttat atttgtcccg gctggaatcc agcctgttta ctattgtccc tataattttg
     4081 cctcttactt tgcgtatctc ttctatactg gtcttaagca cgtttttttg tgttatacca
     4141 acgcttgtta caagaagtac cccgtctaca agtctggaca agagtcttga atcagcaaat
     4201 gccgctatag gagctccatc tattattacg tgctcatatt tttcctggag ggcaattatt
     4261 aattccttca ttttcttcga acctaaaagc tctgccggat ttggaggtat aggtccggaa
     4321 cttataaaat caagtccgtc tatatctgtt ttagttatga cattttccac ttcaccctta
     4381 cctaccagat aggtgctaag tcccattgag tttccattgg atgaatacaa tttgtgcagt
     4441 ctcggctttc tcagatccgc atctattaaa attacattta agcttgactg tatcatggaa
     4501 agtgcgaggt tagaggcaga agtagttttc ccctcaattg gctgtggact tgtaaccaga
     4561 agggcttttg gaggatgatc agcagatgaa tacaatatag aagtccttat taccctaaaa
     4621 gcttctgata caacagacct tggatttgta taaacaatcc tttcaagctt atcaacatct
     4681 gcttcctcag ccatgggtac tgcaccaaga taaggaacat gaggaaattt cttctttaat
     4741 tcttcttcat ctcttagttt ggtactaaaa aattctattc caaatgaaat aaaacatcca
     4801 atcataagtc cgatcattat tgaatagata agattgcctt tcatatcagg tttaaaaggt
     4861 gacctaggca cggttgaata atccacaacc tgtacatttg tctccttaat agcagatgtt
     4921 acttcggttt cttttaattt tgtaagtagt tcattataaa tagatttatt agtatctacc
     4981 tctctttcca atatcctata ttggatagcc tgctcattga gctcagaaga ccttgtcttt
     5041 ttttcttcta ctttcgcttt taatatctct tctttcttta tagtagattg ataatctctt
     5101 tttattgaat ctgcaatttc actaagggta gttttcaact gtccctgaac actatttatc
     5161 tgagaccgga ttttttgtat atcgggaaaa ttaagaccat atacagcaga aagattatca
     5221 tactctgcct taagctgtga atacgaagag cttagctcct gcactcttgg gttttctatt
     5281 acttcgggca aatatgcata attaccatca gttacgtctt taataatagc ttgctttttt
     5341 agtttctctg tttcaagctc agaaagcgca tcggttatct cgacaagctg tttatagttt
     5401 aaatctatat ccttatcaag tgaaataata tcaacagtct tagcaaatgc ggatagctct
     5461 tcttctgcaa gttctatttt agtttttacc tgatctagct gggttcttaa aaactccctt
     5521 gctgattttg atgtgccatg ccttctttca agaagccatt caatatatac atctataacc
     5581 gtatctgcag caagtgcggc gtattgaggg tcagaactca cataactaac ccttgcaagc
     5641 cttgtactgc caacaggacc aacgctaata cctgaaagaa atccaaagac ttgagaatct
     5701 gtaatctttt ttttaggctt tgatgattta gcttctttat tagctgattt attttttttt
     5761 atttgatcct taccataaat tatagattta actgatgtaa taagaccaga tattttatct
     5821 tctttatggc tcttagtgct cttttgctga ttgcgattac ttattttttc gtttagtctt
     5881 tctaatacca aatatcctaa tgtcttactt tttattaaag aaaattgtgt ctggtagtaa
     5941 tcataagtat tggttctttc cgctcgtgcc agatcctcga aagaggttat ttttggaata
     6001 tcaggtttaa tctcaatagt agctgatgct ctatagagag gaacggtagt tagggttttt
     6061 actatagcta ttactaatga tattattata catgcaagta tcagccattt tcttcgtata
     6121 ataatatcaa gatattcttt aacattaata tcatcatctt cattattttc attaccataa
     6181 ttattataat gatattcgct tggataatat tcgtcccatt ccctaatttc tctaccgtta
     6241 gcttttactg gatatctgtt gttatattct gtcatactat attactatca aattagtctt
     6301 ccggaggatc ataaccaaaa ttaaataagc caaaaccaag ccctactctc aatccgtata
     6361 aaatcctctt tgatgtacta aaacctacta taagtgcatc tctatccttt acaagtggat
     6421 ccacagcttc tccgctctga atcttggtta agtcgacagg tctgatttcc ctgttaccat
     6481 tacccaaaaa ccttatgagt ttaatgttgc ttgcatcacc ataaggagct acaccacctg
     6541 ctactgttat aatctgactt attgttgtca gatctttttt gattggatag gctcccgtcc
     6601 tctgcacagc accttcaata tatacattgc cagcaggtgg aacataaact acatcaccgg
     6661 gtttcataac aacatttata tcagaattgc catttaacaa tagttcgtcc agatccacaa
     6721 gataggtttg cttgttacca tttacatctg atctgctgat atatactgtc ctgcccgctt
     6781 tctcctgaaa acctttagca gctacaagaa catccagcaa tgtctgcctt cctgtgatcg
     6841 aatgattacc tggttcttca accaatccga ctacagttac gagtttacta ttatattcct
     6901 cgataaaaac cgtgacatga ggatttttta taaatccttc ctttattaac tggttttcta
     6961 ttttaagctc tgcttctctt gcagaaagcc cgcctacgct tatatttcct agcaagggaa
     7021 gcgtaatgtc tccacgcgag ctgactctaa ccttatccgt aagcttctct gattcaaaca
     7081 cctgaacctc cagaagatca gcaggaccaa ttatatagtc tgcaggtgat gatacaccgc
     7141 ctgacccgct ggacattaaa agctttgtat taagcgcatc attttcctgt ttggtctgaa
     7201 tgtctgcttc ggtcactatt ttagtattct gtacggcacc gcctccgccg caactataaa
     7261 caaccaatgc agtggataat actaaaaggc ttacaaataa tattctacaa atacgcataa
     7321 ccatctcttt gttcatcatt taagtaataa ttacaataaa tatattatga ccctcaccca
     7381 aaagctgtac caggataatt agagatttcc ggctttttgt gttctttaac atactcctcc
     7441 ggcgtcatgt tatctagtgc cgaatggggt ctgtactcat tgtaatctct cctc
//
LOCUS       DBNGSV010000234        14429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1148843, whole genome shotgun sequence.
ACCESSION   DBNGSV010000234 DBNGSV010000000
VERSION     DBNGSV010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1148843"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..838)
                     /locus_tag="AC159U_08555"
     CDS             complement(413..838)
                     /locus_tag="AC159U_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3337149.1"
                     /translation="MSDKKEEILNLNKTFYDALGANDIELMESVWVTDHRAKCIHPGW
                     PLLIGWDPIKKSWENIFVAGGISGVSISDVHVEITDEICWVTCIEHISHVINDKIAVN
                     LIQATNVFEDTGDDNWNIVLHHASSIPITRGYANKETLQ"
     gene            complement(860..1996)
                     /gene="queG"
                     /locus_tag="AC159U_08560"
     CDS             complement(860..1996)
                     /gene="queG"
                     /locus_tag="AC159U_08560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3337150.1"
                     /translation="MDINKITSLIKEKALTTGFDLVAVSAVGRFPENQFYKEWLDKGY
                     AGKMGYLERNSAKREDITQVLPGAKSIICCAVNYNTDYPYSTELDDTDRGWISRYAWG
                     DDYHGSVLDMLKSLNEFCKEICPDSFNSKFYVDTGPVMEKVYSKYSGIGWIGKNTCLI
                     NQGAGSWLFLGEIITDMVLEYDTEAPDRCGTCTRCIDACPTGAIVKPYVLDATKCISY
                     LTIELKDSIPSELREGLENNIYGCDICQDVCPWNSKDNREANITDAPEFQPREGLLNP
                     KLTDIAGMTPDRFREVFRKSPIKRTKRRGLIRNALIAIGNSGNTGFAAEVKNALTDEE
                     PLIRAAAVWALWKLLGDEAKQQLSLMLEHETDSIVISEIDDILK"
     gene            complement(2006..2497)
                     /locus_tag="AC159U_08565"
     CDS             complement(2006..2497)
                     /locus_tag="AC159U_08565"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3337151.1"
                     /translation="MKFLVLRSDLKRKLKYINLLIIDAEIFAAEIKEAGGLLCEKIEP
                     DNLKSLLEKNMRIVLSSNTLNGGYDNLVNGSDIKYIPLDGLSYKEFIERLCADYGLNN
                     TQVAFINKTEQDNDLLSGLNFSVATIDAPLELKTASYYVSNFTGTDTLNEILQIINQA
                     KNG"
     gene            2588..3289
                     /locus_tag="AC159U_08570"
     CDS             2588..3289
                     /locus_tag="AC159U_08570"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3337152.1"
                     /translation="MIPLRDTTKSNSFPFINITLITVNILIFFFQLTLGDRVNEFIYH
                     YGLVPANLFSKTSLSFSDRFFPFISSMFLHGGWMHIIGNMLFLYVFGDNVEDRMGHFK
                     YLMFYVFTGFIAAFFQIATNLSSQIPMVGASGAISGVLAAYLLFFPRAKILTVIPVFI
                     FLQFVHLPAFVYILLWFGFQLLSGIGALSIGGDVGGVAFWAHIGGFVGGLILAKSFQK
                     KGYKLLSNIKTKYYH"
     gene            3296..4540
                     /gene="pfp"
                     /locus_tag="AC159U_08575"
     CDS             3296..4540
                     /gene="pfp"
                     /locus_tag="AC159U_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR3337153.1"
                     /translation="MRKKETLAILTAGGPAPGINGVISAATIEAVNRGLKVIGILDGF
                     KWLIKKDWTKITNLTINNTSRIHNTGGSIIGISRDNPTQSKQSMANVISTLKKLKVDY
                     LIAIGGDGTLFLANELSRASRGRLKIAHVPKTIDNNIALPGYIPTFGFETARHIGTRE
                     VQNIMEDARTTKRWFFVVTMGRKTGHLALGIGRAASATISVIPEEFGRKKISLDSVVK
                     ILAGSVIKRLADGNEHGVAIISEGIANILDENSLSHLQDLQKDEFGRVRFSELDLGQL
                     LKKESTRKLVEMGIDIRIVDKRIGYELRSAPPIPFDAKYTRDLGYSAVKFLLQGGSDA
                     MVTIQRGKMVPIGFDKLIDSRTGKTKIRYVDINTESYEVARKYMIRLEKEDLQDQKKL
                     RKINKVTNISANKFREYFGDIF"
     gene            complement(4549..5061)
                     /locus_tag="AC159U_08580"
     CDS             complement(4549..5061)
                     /locus_tag="AC159U_08580"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3337154.1"
                     /translation="MEKKNNIYLIGFMGAGKTTVGRILSSNIGAVLVDLGLEIEKQQG
                     VAISQIFEEQGEPYFRAIETEMLRDISQRSGQVVSTGGGIVIKDENWDIMKESGVTVY
                     LKASIETLFNRVKHKTTRPLLNVDDPYTKAKELFESRRELYEKSDIILDREGLEPEEV
                     AEAIVKEIEA"
     gene            complement(5045..5698)
                     /locus_tag="AC159U_08585"
     CDS             complement(5045..5698)
                     /locus_tag="AC159U_08585"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3337155.1"
                     /translation="MFTGLVEGIAKVKDLKKGSKEVVLTFESKDVDLKGIALGDSIAI
                     NGICLTVVSFKGSTFSVEASHETLSKTSLGKLRRGSAVNMERSLRVGDRLGGHFVTGH
                     VDGTARISSVTPVGRSVEIWVSVSDELAKYIVEKGSVAIDGISLTVNEVKRESFSVNI
                     IPHTQDKTTLQSAKAGDTVNIECDIIGKYVEKFVSIGKDGDKDKRLRGLLENFGKEK"
     gene            complement(5698..6192)
                     /gene="lspA"
                     /locus_tag="AC159U_08590"
     CDS             complement(5698..6192)
                     /gene="lspA"
                     /locus_tag="AC159U_08590"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3337156.1"
                     /translation="MKKIGFVSIICTVFVILDQISKYLIVKSVPLYGKISLLPFFDIV
                     HIRNPGVAFGFLSNLPENFRFYFFILVFIIALVLISAFIYNTPFKEKIMIVSLSLILS
                     GAIGNSIDRLRQGYVTDFLDFHWFGDPNLHWPPFNVADSCITIGVILLIACTLFFTEN
                     REKA"
     gene            complement(6202..8448)
                     /locus_tag="AC159U_08595"
     CDS             complement(6202..8448)
                     /locus_tag="AC159U_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337157.1"
                     /translation="MDLLDIVLSKKVVVAAAVALVVSVFLTFFPLIGTLGFEYSVIMA
                     FFMSFVSIFISAEYINLDLKKKYPMRKRYSDLISTSFFINFIILLIPFLVGLISSLIK
                     SDCYLREGLAFFLLITTITVFFASTLGLLIGYFFPKRGFFLGSLCVFAIIIYSLYVLY
                     SQPPVFSYNHIFGLFPGPIYDQIILIDEKILTFRAVTICWGIFFLLVLKLIHDYRFRY
                     IGIGTVALFIFISLIITAYKIYENNLGIKHTRHYIQNTIFKDYFETEHFIIYFPLGTE
                     VSQNIELIALDHEWQYAQLTEYLNVSPEKKIVSYIYADEKTRKKIIGAGQTTIANPIH
                     SEIHLVYGFYPHPVLKHELVHILSSEFASGILKISPKVGLIEGLAVAADWDNSDGFDP
                     DQWSAAMLKSDNNIDIQNILGYGFWAAASSKSYTLMGSFVRFLISEYGIEDFKTVYNT
                     GDFSVYETDLSALINQWKDHLGTVEIAKSARALSKHKYSKPGIFDDSCPRKSSHLADV
                     GISEFEKGNYYEAVQSFKKALNYNPRSNRILESLAYSYYYDKDFHNLSKIAERKTRVS
                     QVSRDIILNLIGTYHWTRGDSDKGREIFSSLYNNPLPVDIQNELSIKLDSLTKNDDSQ
                     QGLKQYYMTKKELRRISALDRLTKQYPDYAVPYYLLGKLFFNKWEFDIARDYLLHSER
                     LGLPTDKLKLENSRLLGISLYAGGQYDKARMTFEGIYENRKSGRYGSVAADFIKRSKW
                     AKIYSFNK"
     gene            complement(8485..9639)
                     /locus_tag="AC159U_08600"
     CDS             complement(8485..9639)
                     /locus_tag="AC159U_08600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3337158.1"
                     /translation="MANNLTEKDISEYRKLFPVTEKYIYLDHAGVAPISTRVRDRVDG
                     FINSALYKGVADYDYFMEGVECVRADFAKLLNSGIEEIAFIKNTSHGLSLLAKGIEWK
                     AGDNIIIFDKDFPTNVYTWLDLQRVGVEVRQVPLIDGTVDLNEIKKHIDSNTRLLSVS
                     SVQFTSGYRADLKSIGQICKNNNLIFCVDAIQSLGIVPMDVKELGIDFLAADGHKWLL
                     SPEGTGILYCSERIVKDIHPVLLGWKSIVNDAEYESIDPELKPNAIKFEESSFNVLGI
                     FALGASIELLLEIGIENVCERIQYLGDIIIDEAQKRGFKVNTPVSKDQRAGIISFCGD
                     FDSAQLRDSLLDRNVIVNVRGGGLRVAPHFYNTKDDVHALFKQIDEILLS"
     gene            9785..10924
                     /gene="moeB"
                     /locus_tag="AC159U_08605"
     CDS             9785..10924
                     /gene="moeB"
                     /locus_tag="AC159U_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR3337159.1"
                     /translation="MGLTNDQISRYSRHLIMPEVGVEGQEKLVNSKVLCIGAGGLGSP
                     LALYLAAAGVGNLGILDFDVVDFSNLQRQIIHSEDNIGVLKVESAKERLLELNSDINI
                     TTYNERLSSDNSMEIFKGYDLVIDGTDNFATRYLVNDTCVLLGIPNVYGSIFRFEGQV
                     SVFDAKRGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGIIGTMQAAEAIKLIIGKGSH
                     LIGRLLFMDVLEMKVDELTLRKDKNCPICSENATIKELIDYEEFCGIGRGEEEDAGDM
                     EITIDEFKEIRESNKDFVLLDVREDHEYDICKFHEAKLIPLGELASRVNELDTADEIV
                     VHCHHGMRSLQATRLLRGMGFKKSKSLAGGIDAWASTHDLDMPRY"
     gene            11102..11452
                     /locus_tag="AC159U_08610"
     CDS             11102..11452
                     /locus_tag="AC159U_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337160.1"
                     /translation="MIKKLSIVILLLMVIAAGAVHFWLHKDPPDTVITAENALATKGL
                     VAIGYFNNDLLTTILEYARGEEDPSPFPMPGMDEGLWEDLYSGKVNLKENLDHIVFGI
                     NLSLDKTTITSDIS"
     gene            11681..13264
                     /locus_tag="AC159U_08615"
     CDS             11681..13264
                     /locus_tag="AC159U_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337161.1"
                     /translation="MASNSLENVNMILSRLNQNAQAEANLSKWREFRKGRLASTGFLY
                     PNELIESTTGMTKFLLQKEFTDETDLTRAFIGTSVDYMKAGFRFDVHLQANNQNWIKE
                     TSDKISSTVSEMRKEYAEDFPTLHKLLSGFKVSHDKTLMDISFAMDANTLKTIPDLVG
                     EFIGSLFSLGSASDEESESEERINENPWDYTNNEGFEQMSDFTPEKNWPIPSITDGPF
                     GIDIDSVSLGEKSKLLELNIKSQIRLPKKDENNFMSWFGSGAELTLIIESVKGKDGQE
                     LLRDEYCMEKLEDNFAKKNSEPESGFGYSNNTAHVSKTIRLIPGTSLKDIDKVIGKVS
                     FTVPSKVKKIPVTLKKGTVVEHNDMRFYLSSIKQQSISYQISGDKDKLLEVRALNDKG
                     QTLQSSFGTSSSYRNVKSFRGDIKGLEIYVLDKKTENERDFELKAEDIFKVKYNEEKN
                     TLPILVEPGVVKKSKWKRLSSKKVSLATLNYYERPIAERDKDIVAEHTHSPIIITAEH
                     KFNQKWYSQPMLQVYMPFI"
     gene            13424..13852
                     /locus_tag="AC159U_08620"
     CDS             13424..13852
                     /locus_tag="AC159U_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337162.1"
                     /translation="MVDQIGIDLDLETGTKIETLEGKLTFRLPQKISRTEIEISEIGK
                     PVNINEDTKITIREINRGFIPRLKIDFEGKTKKLINIIAITDTGKRIFPAQTRLENDI
                     WEIQYDLGQTFTHLEVVMADEQAVIEYPFNLKPEYLEEQN"
     gene            14040..>14429
                     /locus_tag="AC159U_08625"
     CDS             14040..>14429
                     /locus_tag="AC159U_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3337163.1"
                     /translation="MTDKHTLHVLKTVFGYNTFRPLQSEIISNLLDKRDTMVIMPTGG
                     GKSLCYQIPGLLFEGLTVVVSPLISLMKDQVEQLTALGAPAVYLNSSLSAGEYQKNVG
                     SVRAGKVKLLYVAPETLLTSAMSDLLAT"
BASE COUNT         4591 a         2878 c         2743 g         4217 t
ORIGIN      
        1 cctgctccaa aagctttaat acaagacaaa aaaaccacga actatgaaca aactttcact
       61 ccaactcact gacaaaatgt taacaaattc taactttaaa actggaccta aattctgggg
      121 cttgacaccc aaattgcaat ctacagaagg attacccgta ctacaaccac aacaggctcc
      181 tgagagtcct ccgccggcaa gtgcgttttc aatcattgat ggcagaaata aaatggataa
      241 agcaaataat gaaattataa aaatcctgtt tatgttcatg gttttttcct tatagaatat
      301 cgagatatga agttataata ctgatcctcc ttaatacaga attatgctat cacatctgga
      361 tttttgagtc aatgcttttg ttttaatttg aattaaaggt gattaccaag gatcattgca
      421 gagtttcttt attggcatag ccccttgtga tggggatgga tgaggcatga tgaagcacga
      481 tgttccagtt gtcatctccg gtatcttcaa acacgtttgt ggcctgaatg aggtttacgg
      541 cgatcttgtc atttattaca tgagatatat gctcgatgca tgtaacccag cagatttcat
      601 ctgtaatctc aacatgaaca tctgaaatgc tcaccccgga tatcccgcct gctacaaata
      661 tgttctccca gctcttttta ataggatccc atccaatgag aagcggccag cccgggtgga
      721 tacactttgc cctgtggtca gtaacccaca cgctttccat aagctctata tcgtttgcgc
      781 caagggcgtc ataaaacgtt ttattaagat ttaatatttc ttctttcttg tctgacatct
      841 ttttatattt ctctttggat tattttaata tgtcatctat ttcactaatt acaatactat
      901 ctgtttcatg ctccaacatc aaagataatt gttgttttgc ttcatctcca agaagtttcc
      961 aaagcgccca gacagccgct gcccttataa gaggctcttc atcagttagc gcatttttca
     1021 cttctgcagc aaacccggtg ttacccgaat ttcctatagc tataagagcg tttcttataa
     1081 gcccccttct cttggttctt tttatggggc tcttcctaaa tacctctcta aacctatccg
     1141 gtgtcatacc ggcaatatca gtaagctttg gatttaaaag cccctccctt ggctgaaact
     1201 ccggagcatc tgtaatattc gcttcacgat tatctttact attccagggg catacatcct
     1261 ggcatatgtc acagccgtag atattgtttt ccaacccctc tcttagctca gagggaatgg
     1321 agtcttttag ctctattgta agataagaga tgcattttgt cgcatcaagc acatagggct
     1381 ttacaattgc acctgtcggg caggcatcaa tgcaccttgt gcatgtgccg catctgtcgg
     1441 gagcctcggt gtcgtattca agcaccatat cggttatgat ctcaccaaga aaaagccacg
     1501 accctgcccc ctggtttata aggcaggtgt ttttccctat ccagcctatt ccagagtact
     1561 ttgaataaac tttttccatc acagggccgg tatctacata gaatttggaa ttaaacgaat
     1621 ccgggcaaat ctccttacag aattcattta gggattttag catatctagc acactaccgt
     1681 ggtagtcatc tccccatgcg tatcgcgata tccatcccct gtctgtatca tcaagctcag
     1741 tggagtatgg atagtcagtg ttgtagttaa cggcacagca gataatggat ttggcacctg
     1801 gaaggacttg ggtaatgtct tcccttttgg cagaatttct ttcaagatac cccatcttgc
     1861 cggcataacc cttatcgagc cactctttat aaaactggtt ttcaggaaac ctgcccaccg
     1921 ctgaaacggc cacaagatca aatccggtgg taagcgcttt ttccttaata agagatgtaa
     1981 tcttatttat gtccacagac tgagtttaac cgttttttgc ctgattgatt atctggagta
     2041 tttcattcaa tgtatcggta cctgtaaaat tgcttacata ataggaagct gtttttagct
     2101 cgagtggggc gtctattgta gcaactgaaa aatttagacc cgagagcaga tcattatcct
     2161 gctcggtttt gtttataaat gccacctggg tgttatttag cccgtagtca gcgcagagtc
     2221 tttcgataaa ctctttatat gaaagcccgt caagaggtat atatttaata tcacttccat
     2281 ttacaagatt gtcgtagcct ccgtttagtg tgttggaact gagcacaatt ctcatatttt
     2341 tttcgagcag actcttaagg ttatcgggct caatcttctc acaaagcagc ccaccggctt
     2401 ctttgatctc cgctgcgaat atctctgcat ctataatgag aagatttata tattttaact
     2461 ttctttttaa atccgatctt aaaactagaa atttcatttt tataaattac tttaataaca
     2521 atgtgttagt cttagatgta cctagtttaa ataatgtata taaatgaata ttttatccgg
     2581 tcaataaatg ataccactta gggataccac aaaatcaaac tcgtttccgt ttattaatat
     2641 aacattaatt accgttaaca tattaatatt tttcttccag cttacgcttg gagaccgcgt
     2701 aaatgaattt atataccatt acggcctcgt accggccaat ctgttttcaa aaacgagcct
     2761 gtcattttcg gacaggttct ttccatttat aagctctatg ttccttcacg gtggttggat
     2821 gcacataatc ggcaacatgc tctttttgta tgtatttgga gacaatgtag aggacagaat
     2881 gggccatttt aagtatctga tgttctatgt gttcactggg tttattgcag cattttttca
     2941 gattgcaacc aacctcagct ctcagatacc aatggtggga gcaagcggcg caatatccgg
     3001 ggttttggcc gcatatctgc tgttctttcc tagagcaaag attctcactg taattcccgt
     3061 atttattttt cttcaatttg tccatcttcc ggcatttgta tatattctgc tctggtttgg
     3121 ttttcagctt ctaagcggca taggagcgct ttctattggg ggtgatgttg gcggagtcgc
     3181 attctgggct catatagggg gatttgtagg agggttaatt ttagccaaat catttcagaa
     3241 aaaaggatat aaacttctaa gtaatattaa aaccaagtat tatcattaga gagctatgag
     3301 aaaaaaagag actttagcaa tacttacggc aggcggccct gctccgggaa taaacggggt
     3361 tataagcgct gccacaattg aggctgtaaa caggggactg aaagtcattg gaattctgga
     3421 cggattcaag tggcttataa aaaaagactg gaccaagatt actaatctta ctattaataa
     3481 tacctccagg atacacaaca ccggtggctc gataattggt atctcaagag acaatcccac
     3541 tcagtcaaaa cagagtatgg caaatgtcat atcaacgctc aagaaattga aagtagacta
     3601 tctcatagca attggaggag atggaacact ttttttagca aacgagttat ccagggcaag
     3661 caggggcagg ttaaagattg cacacgttcc aaagactata gacaacaaca tcgcgcttcc
     3721 aggatatatt cccacttttg gatttgagac agcaagacac attggcacaa gggaagtaca
     3781 aaacatcatg gaagacgcaa ggactacaaa gaggtggttt tttgttgtaa caatgggaag
     3841 aaaaacagga cacctggcgc ttgggattgg aagagctgca agcgctacga taagcgtaat
     3901 acccgaagag tttggaagaa agaaaataag tcttgacagt gtcgtaaaga tactggcggg
     3961 gtctgtcatc aaaaggcttg ccgacggcaa tgaacacggg gttgcaatta tatcggaggg
     4021 tatagccaat atactcgatg aaaacagcct ctctcacctg caagatcttc aaaaggatga
     4081 atttggcagg gtacgttttt cagaattaga tctgggacag ctacttaaaa aagaatctac
     4141 aaggaaatta gtagagatgg gaatagatat aaggatagtg gacaaaagaa tcgggtatga
     4201 gctgcgctct gccccaccca tcccatttga tgcaaagtac acaagggacc ttggctacag
     4261 cgcagtaaaa tttttactgc agggcggaag tgatgccatg gtgacaattc aaaggggcaa
     4321 aatggtacct atagggtttg ataagctcat tgatagccgc accggtaaaa ccaaaataag
     4381 atatgttgat ataaacacag agtcttatga agtggcaaga aaatatatga taaggctaga
     4441 aaaagaagac cttcaagatc agaaaaaatt aagaaagata aataaagtca ccaatatttc
     4501 ggcaaataag tttagggaat attttggaga tatattctaa tatcataatt aagcttcaat
     4561 ttcttttaca atagcctcgg ctacttcctc aggctcaagc ccttccctgt ccaggattat
     4621 atctgatttt tcgtagagtt ctctgcgtga ttcaaaaagc tcttttgcct ttgtatatgg
     4681 atcatccacg tttagaagcg gtcttgtggt tttgtgtttt actctgttaa acagggtttc
     4741 aatagaggct tttaggtaaa ccgtaacacc tgattctttc ataatatccc agttctcatc
     4801 tttaatcact atcccgcctc cggtcgatac aacctgaccg gacctttgag atatatccct
     4861 tagcatctct gtttcaattg ctctgaaata tggctcaccc tgctcttcga agatttgtga
     4921 aatggcaaca ccctgctgtt tttcaatttc taaacccaga tccaccagga ctgcaccaat
     4981 attgctggaa agaattctgc ctacggtagt tttgcctgcc cccataaatc ctataagata
     5041 aatattattt ttcttttcca aagttttcca aaagtcctct aagtcttttg tctttatctc
     5101 catcttttcc tatcgagaca aatttctcta catacttacc tataatatca cattcgatat
     5161 tcacggtatc acccgctttt gcactttgta aagtggtctt atcctgcgta tgaggaataa
     5221 tgtttactga aaaagattcc cttttaacct catttaccgt gaggcttatt ccgtcaattg
     5281 ccactgagcc cttttctaca atatatttcg ctaactcatc tgacacagaa acccagattt
     5341 ccaccgacct gccaacaggg gttaccgagc ttatcctggc agttccgtcc acatgaccag
     5401 ttacaaaatg acctcccagc ctgtcgccaa cccttagcga tctttccatg ttaactgcag
     5461 atcccctgcg aagcttgcca aggctggttt tactaagtgt ctcatgggac gcctctacag
     5521 aaaatgtact gcctttaaaa gaaaccactg ttagacaaat tccatttata gcaatgctgt
     5581 caccaagcgc tataccctta agatcaacat ctttggactc aaaagtgaga acaacttctt
     5641 ttgatccttt ttttagatct tttacttttg ctatcccttc tacaagacct gtaaacatta
     5701 tgccttttcc ctattttcag taaaaaacaa agtgcatgct attaatagta taaccccgat
     5761 tgtaatacat gaatctgcta cattaaaagg aggccagtga agattaggat ctccaaacca
     5821 gtgaaagtcc aaaaagtctg tgacataacc ctgccttagc ctgtctatgg aatttcctat
     5881 agcaccgcta agtattaaag agagcgaaac gatcatgatc ttctctttaa acggagtgtt
     5941 atatataaaa gcagatatca aaaccaatgc tataatgaaa acgagaataa agaaatagaa
     6001 cctgaaattt tcgggaaggt tacttaaaaa tccaaatgcc accccggggt ttctgatatg
     6061 aacaatatca aaaaacggca gtaatgaaat ttttccatag agcggaacac tctttactat
     6121 cagatatttg gatatctgat ccagtattac aaatactgtg caaattatgc ttacaaaacc
     6181 aatttttttc atactaatga ctcacttatt aaatgaataa atttttgccc atttcgatct
     6241 tttaataaaa tcggcagcca cactgccata tcttccactt tttcgatttt cataaatacc
     6301 ctcaaacgtc atacgtgctt tatcatactg cccgccggca tataaagaaa taccgagtaa
     6361 tcttgaattt tccagtttta atttatctgt tggcaggcca agcctctcag agtgcagcag
     6421 gtagtccctt gcaatatcaa attcccattt attaaaaaac agttttccaa gcaggtagta
     6481 aggaactgca taatcggggt attgctttgt taatctatct agtgccgaga tgcgcctaag
     6541 ctcttttttt gtcatatagt attgttttag gccttgctga ctgtcatcat tttttgtaag
     6601 tgaatcaagc tttatactga gttcattttg aatatcgaca ggcaagggat tattgtacag
     6661 gctgctgaaa atttcacggc ctttatccga atcacctctt gtccagtgat atgtaccgat
     6721 aaggtttaaa ataatatctc gagaaacttg cgacactctt gtttttcttt cggcaatttt
     6781 tgataggtta tgaaaatctt tatcataata ataagaatat gccagagatt ccaaaattct
     6841 gttacttctt ggattataat tcaaagcttt tttaaatgac tgtacggcct cataataatt
     6901 gcccttttca aactctgaga tacctacatc ggcaaggtgc gaacttttac ggggacagct
     6961 gtcatcaaat ataccaggtt tactgtactt atgttttgaa agagcccggg cagatttagc
     7021 aatttctaca gttcccagat gatccttcca ctgattaatt aaagcgctta agtcagtttc
     7081 atacacacta aaatcaccgg tattataaac agtcttaaaa tcttcaatac catattcact
     7141 tatcaaaaac cttacgaaag atcccataag agtatagctt ttagaagaag ccgccgccca
     7201 gaatccataa cctagaatat tttgtatatc gatattatta tcacttttaa gcatggcggc
     7261 agaccactga tcgggatcaa atccatcgct gttatcccag tctgcagcaa cggccagtcc
     7321 ctcaataaga ccaacctttg ggcttatttt caatattcct gatgcaaatt cggatgacaa
     7381 tatatgcaca agctcatgct ttagcacagg atgtgggtag aaaccgtata ccagatggat
     7441 ttcactgtgt atggggtttg caatggtagt ctgtccggct cctataattt tctttctggt
     7501 tttttcgtct gcatatatgt atgagactat tttcttttcg ggagaaacat taagatactc
     7561 agtcagctgt gcgtattgcc attcatgatc gagtgcaatt agctctatgt tttgagatac
     7621 ctctgtacca aggggaaaat aaataataaa atgctctgtc tcaaaatagt ccttaaaaat
     7681 agtgttttgg atatagtgcc tcgtgtgctt aattccaagg ttgttttcat atatcttata
     7741 agctgtgatt ataaggctta taaatataaa gagcgcaacg gttcctatac ctatatatct
     7801 aaacctgtaa tcatgaataa gttttaaaac gagtaaaaag aaaatccccc aacatattgt
     7861 taccgctcta aaagtaagaa ttttttcatc aatgaggatt atctggtcgt atattggccc
     7921 tggaaaaagg ccgaatatat gattataaga aaacacaggc ggctgggagt aaagtacata
     7981 tagggaataa ataattatgg caaacacgca aagcgagccc agaaaaaatc ctctttttgg
     8041 aaagaaatat ccaataagaa gccccagggt cgaggcaaaa aacactgtta tggttgtgat
     8101 aagaagaaaa aatgcaagcc cttctcttag ataacaatct gactttataa gtgacgagat
     8161 aagacccacc aaaaacggga tcaacaaaat aataaaatta atgaaaaaac ttgttgagat
     8221 aaggtctgaa tatctctttc tcataggata ctttttctta agatccaggt ttatatattc
     8281 agccgaaata aatatagaca caaaggacat aaaaaacgcc ataatgacag agtattcaaa
     8341 cccaagtgtg ccaatgagcg gaaagaaagt gagaaacacc gaaaccacca gcgctacagc
     8401 ggctgcgaca actacttttt ttgagagtac tatatctaat aaatccaata cactttccta
     8461 ttgagtgtca taacggagat aaaatcaaga taataaaatc tcatcgattt gtttaaaaag
     8521 ggcatgtaca tcatcttttg tattataaaa atgaggagca accctaaggc ctcctcctcg
     8581 cacatttaca ataacatttc tatctaataa tgagtctctt aactgagcag aatcaaaatc
     8641 tccgcaaaat gatataatcc cggccctttg atcttttgat accggagtgt tcactttaaa
     8701 acctcttttc tgagcctcat ctatgataat atctccaaga tactgaattc tttcacacac
     8761 attttctatt ccgatttcaa gaagtagctc aatacttgct ccaagggcaa agataccaag
     8821 cacattaaac gatgactctt caaactttat ggcatttggt ttaagctctg ggtcgattga
     8881 ttcatactct gcgtcattca caatgctttt ccatcccaga agtactggat gtatatcctt
     8941 tacgatcctc tcactgcaat acaatatccc cgtaccctct gggcttagaa gccacttatg
     9001 cccgtctgct gcaaggaaat caattccaag ctcttttaca tccataggta ctatgccaag
     9061 actctgaatg gcgtctacac aaaaaatcaa attgttgttt ttgcatattt gtccaataga
     9121 ttttaagtca gccctataac cacttgtaaa ctgtacagag cttacactca gaagccttgt
     9181 attagaatct atatgttttt taatctcatt taaatccacc gtaccgtcta taagcggtac
     9241 ctgccttacc tccactccca ccctttgcag atcaagccat gtatatacat tagtgggaaa
     9301 atccttatca aagataataa tattgtcacc ggctttccat tctattccct ttgcaagaag
     9361 agacagccca tgggaagtgt tctttataaa tgcaatctct tcgataccag aattcaggag
     9421 tttagcaaag tcggccctta cgcattccac tccttccata aaatagtcat aatcagccac
     9481 tcctttatat agagcagagt ttataaaacc atctactctg tcacgcaccc ttgtggatat
     9541 gggagctact ccggcatggt cgaggtatat atatttttca gtaacgggga atagttttct
     9601 gtactccgat atatcttttt ctgttaagtt atttgccatc tctaatctat tattaattga
     9661 gccgtacact actgccgaat ctttttgtag taaataaaat caaaataata accagaccat
     9721 attgaaaaat taaattaatg tattagattg tacggtctaa acacataaat ggaaggtatt
     9781 ttaaatgggc ttaaccaatg atcaaataag caggtactca agacacctca taatgcctga
     9841 ggttggcgta gaaggacagg aaaaactagt aaactcaaaa gtgctgtgca taggcgcagg
     9901 cgggctcggt tctccactag cactttacct tgcagcagca ggcgtcggaa atttgggaat
     9961 actggatttt gatgtagtgg atttcagcaa cctgcaaaga cagataattc atagtgaaga
    10021 taatataggt gttctaaagg tagaatcggc aaaggaacgc ctgctcgagc ttaactctga
    10081 tataaacata actacatata atgaaagact gtcgagcgat aactcgatgg aaatttttaa
    10141 gggctacgac ctcgttatcg acggcacaga taattttgct acaagatatc ttgtcaatga
    10201 cacttgtgtg cttcttggaa taccaaacgt gtacggaagt atattcaggt ttgaaggcca
    10261 ggtaagcgtg ttcgatgcaa agagaggccc gtgctacagg tgtctttatc ccgagcctcc
    10321 acctccggga ttagtaccaa gctgtgcaga gggcggagta ctgggaattc ttcccggaat
    10381 tataggtacc atgcaggcag ccgaagcaat taagcttatc atcggaaagg gaagccatct
    10441 gataggaaga cttcttttca tggatgtatt ggaaatgaag gttgatgaac taaccctacg
    10501 taaagacaag aactgcccta tatgcagtga aaatgctaca attaaggaac ttatagatta
    10561 tgaagagttc tgcggaattg gcaggggcga ggaagaagac gcaggggata tggaaatcac
    10621 aatagatgag tttaaggaaa ttagagaatc caataaagat ttcgtgcttt tggacgtaag
    10681 agaagatcac gaatacgata tctgtaagtt tcatgaagca aaactcatcc cgcttggaga
    10741 acttgcttca agagtaaacg agctggatac ggcagacgag atagtagtgc actgccatca
    10801 cggcatgaga agcctgcagg ctacgagact tctaagaggt atgggtttta agaaatccaa
    10861 gagccttgcc gggggaattg acgcctgggc aagtactcac gatctggata tgcctaggta
    10921 ttaatataaa ttcaagacca gagggcagta aaccagctgc cttctggaaa ttaaaccata
    10981 agactaaata gctagtccct attttactca acactcctca gccaagaatc ctttctaaaa
    11041 acaattgcat cctttataat tggaaggtta atacatattg tttgaagggg catattatcc
    11101 tatgataaaa aaactctcga ttgtcatact tcttctcatg gtcatagcgg caggagcggt
    11161 tcatttctgg cttcataaag acccgcctga tacggtgatt acagctgaga atgcacttgc
    11221 aacaaaaggg cttgttgcta ttggttattt taataatgac ctcctgacta ctatactgga
    11281 atatgcaagg ggtgaagaag acccatctcc atttccaatg ccgggcatgg atgaaggact
    11341 atgggaagat ctgtactcag gaaaagtgaa cctgaaagaa aacctcgacc acatagtatt
    11401 tggaattaat ctttccttgg ataaaaccac tataacatca gacatttctt agcatacaaa
    11461 attaggtgga ggtatcatag cacttggaaa tttcgattgg aataatatca ggaatgctat
    11521 acaggatagt tatattatag aagataaaca aaacaacaac tataaaatca ctgcaataaa
    11581 gcaagagtca gaaaaggcag aaagagtatg cccgggggat gaaaagacaa atcccaaaaa
    11641 agatacagag acatacataa atattaccaa gacagcgatt atggcttcaa atagtttaga
    11701 aaatgtgaat atgattctaa gcagactgaa tcagaacgca caagctgagg caaacctgtc
    11761 caagtggaga gaatttagaa aaggacgtct agcaagcact ggatttctgt atcccaatga
    11821 attgattgaa tctactaccg gcatgacaaa atttttgcta cagaaagaat ttactgatga
    11881 gactgactta accagagcat ttataggaac atcagttgat tacatgaaag caggatttag
    11941 atttgacgta catttgcagg caaataacca aaactggata aaagagactt ctgataaaat
    12001 ttcctcaact gtaagtgaaa tgagaaaaga gtacgcagag gattttccaa cattacataa
    12061 gctcttatcc ggatttaaag tatcccatga taaaactctg atggatataa gttttgcaat
    12121 ggatgcaaac actctaaaaa caatcccaga cctagttggt gaatttatag gctcattatt
    12181 ctcactaggc tctgcatctg acgaagaaag tgaatctgaa gaaaggatta atgaaaatcc
    12241 gtgggattac acaaacaatg aaggatttga acagatgtct gattttactc cggagaagaa
    12301 ctggccaatt ccgagtatta ccgatggtcc ctttggaatt gatattgact cggttagctt
    12361 gggagaaaag agcaaacttc ttgagctgaa tattaagtca cagatacgtc tgcccaaaaa
    12421 ggacgaaaac aattttatgt cctggtttgg ttcaggagct gagcttacac ttataattga
    12481 atctgtgaag ggtaaggacg ggcaggagct tcttagggat gagtattgta tggaaaagtt
    12541 ggaggacaat tttgcaaaga agaacagtga accggaatcc ggctttggtt attcaaataa
    12601 caccgcacat gtaagtaaaa caatacgctt aatccctggt accagtctaa aagacataga
    12661 taaagtaatt gggaaggtaa gttttactgt tccatcaaaa gttaaaaaaa ttcccgtcac
    12721 tttaaaaaaa ggtaccgtag tcgagcataa cgatatgcgt ttttacttaa gcagcataaa
    12781 acagcagagc ataagctatc agatatctgg agacaaagat aaactactgg aagtaagagc
    12841 acttaacgac aagggccaga cacttcagtc aagctttggc acctcctcat cgtatagaaa
    12901 tgttaagagt ttccgtggag atattaaggg ccttgagatt tatgtattgg ataaaaagac
    12961 agagaatgaa agagattttg aattgaaggc agaggacata ttcaaggtta aatataatga
    13021 agagaaaaat actctaccta tactggttga acccggagta gttaaaaaga gtaaatggaa
    13081 aagacttagc tcaaaaaaag ttagtctagc cacacttaac tactacgaac ggccaatcgc
    13141 ggaaagggat aaagatattg tggctgaaca cactcacagc ccaattatta ttactgcaga
    13201 gcataagttt aatcaaaagt ggtacagtca accaatgtta caggtttata tgccatttat
    13261 ttgagaatta aataaaaacc tgagtgcact agaagttcac ataacaaaac ctctaaatca
    13321 gaaaaccgag aaggaatttt cgaaattttc agaaattaga gctccacaga gtaccaaaac
    13381 gggtgagtat gttacgtcat acagccataa agactcaccc tatatggttg atcaaatcgg
    13441 cattgacctt gaccttgaaa caggtaccaa aatcgaaaca ctagaaggga aactaacctt
    13501 ccgcctgcct cagaaaattt ccagaactga gattgaaata tcagaaatcg gtaagcctgt
    13561 aaatattaat gaagatacta aaattacaat acgtgagatt aatagaggat ttataccacg
    13621 acttaaaatc gactttgaag gcaagacaaa aaagcttatt aatattattg ctataactga
    13681 tacaggcaaa cgcatattcc ctgcgcagac aaggctagaa aatgatatat gggaaataca
    13741 gtatgatctt ggacagacat tcactcacct cgaagtcgtt atggctgacg agcaggctgt
    13801 aatagaatat ccgtttaatc ttaagcctga atatttagaa gaacagaatt gatttcggac
    13861 cagaacatgg ctatagatta aatttaagtt aggtaaggta agacaagaag atagcaaata
    13921 atcttctttt tgtagtatta aattacatct acagtcatat tattactttc aaattgagta
    13981 atatctaata ttattacaac ttcttattca gttgtatctc cctaaaggaa atatttggaa
    14041 tgacagacaa acacacactt catgttctga aaacagtatt tggttacaat accttccggc
    14101 cactgcagag tgaaattatt tctaatttgc tcgacaaacg tgacacaatg gtgatcatgc
    14161 ccacaggtgg gggcaagtcg ctctgttatc agattccagg gttattattc gaaggactca
    14221 cagttgtagt atctccgctt atatcactca tgaaagatca ggtagagcag ctcaccgccc
    14281 ttggtgcgcc tgccgtatat ctaaacagtt ctctaagtgc cggagaatat caaaaaaatg
    14341 taggctctgt acgcgcaggt aaagtaaaac ttctgtatgt tgctcctgag acattgctca
    14401 cttctgcaat gtccgatttg cttgccact
//
LOCUS       DBNGSV010000235         4281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_942471, whole genome shotgun sequence.
ACCESSION   DBNGSV010000235 DBNGSV010000000
VERSION     DBNGSV010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_942471"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..949)
                     /locus_tag="AC159U_08630"
     CDS             complement(125..949)
                     /locus_tag="AC159U_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3337164.1"
                     /translation="MASLTFKGGSYYGVFSKAGKKKWLKIGRVDKKEAKKILKQLELD
                     YSKDRLNLSSPLVPTLYQYLNKYFVYSKTNKAHSTYTIELEITKTIKKYFGDIELSKI
                     DSISIENYKSHRKSLGLKPATINKELSLLRFMLNKALEWKYIDKTPKLKLLRLPKEPV
                     KYLNTIEINKILDNCSEWLKPMVIVMLNTGMRIGETLNLRFKDIDYDNRKIMVRSSKT
                     NNFRVIPMNEELFRILRWLKLNYVNFKNQKVTLRQPQQREYLFCHSNGNKLKTIKN"
     gene            complement(1080..1165)
                     /locus_tag="AC159U_08635"
     tRNA            complement(1080..1165)
                     /locus_tag="AC159U_08635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1129..1131),aa:Leu,seq:gag)
     gene            complement(1296..2516)
                     /locus_tag="AC159U_08640"
     CDS             complement(1296..2516)
                     /locus_tag="AC159U_08640"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3337165.1"
                     /translation="MEIITSLKQKNDTKIVLCVLDGLGGLPVNGKSELQFANTPNLDA
                     LSEHSALGLQVPVATGVTPGSGAAHLGLFGYDPLKYDIGRGVLEVLGLGLNMDSHDLA
                     IRGNFATVEYKDGKPVVTDRRAGRIATEENKRITEKIRSKIDEINDIKVTITSGLEHR
                     FALILSFPLSLPEGSDQIPDTDPQEVGKSPIIPKGKNVTAQKVAKELEKIISNIVDVI
                     KDEEKANYILLRGISIFPKLQSYEHRYGLDAGCIAAYPMYKGVSKLVGMEVLHVEGVT
                     VESEVDALVRDFSKKDFFYMHIKKTDSYGEDGNFEAKAGVIEEFDSQLPRILDLKPDV
                     LLITGDHSTPAAMKAHSWHPVPVLLSSNFCFGNNKDGFSENGCRAGELGIVKSTELMP
                     LMMAHAGRLKKYGA"
     gene            complement(2550..3320)
                     /locus_tag="AC159U_08645"
     CDS             complement(2550..3320)
                     /locus_tag="AC159U_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3337166.1"
                     /translation="MKVIILGCATSTGVPIVGCSCNVCLSDNPKNKRTRCSVFIQVNN
                     INLLIDTSPDLRFQALRHDITLIHAVLYTHSHADHTHGIDDLRTYNFINKMTIPCYGN
                     ALTVNNLKNNFAYIFDGIYSAGGKPKLILNEVGGDFKVEGVTITPIDINHADWIILGY
                     RIGNIAYLTDCSGIPDYSYEKLQNLDVLIIDALRPTPHAAHFSVDQAIEQVEKIKPKK
                     AVFTHMGHELDYKDLKSRLPSNIMPAYDGMEFTLDDPN"
     gene            3489..>4281
                     /locus_tag="AC159U_08650"
     CDS             3489..>4281
                     /locus_tag="AC159U_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337167.1"
                     /translation="MALSAQSRAADKSEELFDNANSLYKTILKDTSKAQNVEIWQKLA
                     DAFYGIFKSYNNSQKAPTALFISGKLYEKMGYNFNSKDYLKKSIDVSRELTIFYPKSN
                     YADDAQIRIARITEGWDKTQAFIEYDKVLNEFAGGDMVYAATKKVKQLDKYKPKGYSY
                     LKNKRNTTIVSQSEIKNTKGLSKVTNIRNFSLRDYTRVVIDMEGQKRYKSHLLKPDPK
                     HGKPPRLFVDIDGTYIDPNLKIPSLEAGLLEQIRFGRNTDNKVRVV"
BASE COUNT         1328 a          838 c          744 g         1371 t
ORIGIN      
        1 gaggagagat tacaatgagt acagacccca ttcggcacta gataacatga cgccggagga
       61 gtatgttaaa gaacacaaaa agccggaaat ctctaattat cctggtacag cttttgggtg
      121 agggtcagtt cttgattgtc ttcaatttgt tcccattaga gtgacaaaag agatattctc
      181 tttgttgagg ttgcctaagg gttacttttt ggttcttaaa atttacataa tttaatttta
      241 accacctgag tatacgaaat aattcttcat tcataggtat aactctaaag ttatttgttt
      301 tagaacttcg caccatgatt ttcctattat cataatcaat atccttaaat cgaaggttta
      361 aagtttcacc gatcctcata cctgtattta acattactat taccatgggt ttaagccact
      421 ctgagcaatt atctaatatc ttatttattt ctatagtatt gagatattta acaggctcct
      481 taggtaaccg aaggagttta agcttaggtg ttttgtctat gtatttccac tccaatgcct
      541 tatttaacat aaacctaagt agtgacaact ctttatttat agtagctggt tttaaaccta
      601 gagatttcct atgagattta taattttcta tagagatgga atcaatttta gatagttcta
      661 tatcaccaaa gtattttttt attgtcttag tgatctccaa ttctatagta taagtggagt
      721 gagctttatt agtcttagaa tatacgaagt atttattaag gtattgatag agtgtaggta
      781 ctaaaggtga acttaagttt aacctgtctt tactatagtc taactcaagt tgctttaata
      841 tcttctttgc ctctttctta tcaaccctgc caatcttaag ccactttttc ttacctgctt
      901 tactgaagac cccataataa ctaccaccct taaatgttaa acttgccata ataattaccc
      961 cttgtttttg actagagtaa ccattaacta agttcaactc ggttagaata tggttagaaa
     1021 aagaattatg gcttaagtta atagatggtg aaaagtgtcg taacccactg tagttattgt
     1081 ggtgccgaag gggggattcg aacccccacg cccgtaaggc actaccccct caagatagcg
     1141 cgtctgccag ttccgccact tcggcatgtt taaaaaggaa aattaagtta gcatagaaag
     1201 tttaaaattc aagactttta agtaacaccc aatatgaaat ttaaacctca ttctggataa
     1261 acttcaacac ttcataagat agtgctgtaa acaaatcatg ccccatattt cttaagcctt
     1321 cccgcatgag ccatcattag aggcataagc tcagttgatt ttacgatccc aagctcccct
     1381 gccctgcagc cattttctga aaaaccgtct ttattattac caaagcagaa gttagaactt
     1441 agcagtaccg gtaccggatg ccagctatgt gctttcattg cagcaggggt agaatggtca
     1501 ccagttataa gcaggacatc tggttttagg tcaagtatcc ttggaagttg tgaatcaaac
     1561 tcttctataa cacctgcctt agcctcaaag ttaccgtctt caccataact gtcggtcttt
     1621 ttaatatgca tataaaagaa atctttttta ctaaaatccc ttacaagtgc atcaacctcg
     1681 ctttccacag ttaccccttc aacatgaaga acctccattc cgacaagctt ggatacccct
     1741 ttatacatag gataggcggc gatgcagcct gcatcaagtc catatctatg ctcataactt
     1801 tgaagtttag gaaatataga tatgcccctg aggaggatat aatttgcttt ttcttcatct
     1861 tttataacat ctacaatatt gcttattatc ttctcaagct cttttgctac tttctgagcc
     1921 gttacgtttt taccttttgg aatgatcgga gatttaccaa cctcctgcgg gtcggtatcc
     1981 ggtatttggt ctgagccttc aggcagtgag agcggaaaag aaagaataag cgcaaaccgg
     2041 tgttctagac ctgaggttat cgttaccttt atatcattga tctcatcaat ttttgatctg
     2101 atcttttcag ttattctttt gttctcttca gtagcaatcc tgccggctct tctgtctgta
     2161 accactggtt ttccgtcttt atactcaacg gttgcgaagt taccccttat cgcaaggtca
     2221 tgagagtcca tattaagacc aagcccaagc acctcaagaa cccccctccc tatatcatat
     2281 ttaagcggat cataaccaaa aagcccaagg tgagccgcac cgcttcctgg agtaacaccc
     2341 gttgctactg gtacctgaag accaagagca gagtgttctg aaagagcgtc aagatttgga
     2401 gtgtttgcaa attgcagttc agattttcca tttaccggaa ggcctcctag accgtccaga
     2461 acgcacaaaa caattttagt gtcgtttttc tgctttaggc ttgtaatgat ctccattaat
     2521 taattctccc ctactcttat aattatacat caatttggat catcaagtgt aaattccatc
     2581 ccatcgtatg cgggcattat attgctcggc agacgagatt tcaagtcctt gtagtcaagc
     2641 tcatggccca tatgtgtaaa tactgctttt ttgggtttaa ttttctctac ttgctctatg
     2701 gcctgatcaa cactgaagtg tgctgcatga ggggttggcc ttagagcatc aataattaac
     2761 acatcgagat tttgcagctt ttcatatgaa taatcaggaa tgccgctgca gtctgtaaga
     2821 taggcgatat ttccaatcct gtatccaagt ataatccagt cagcatggtt tatatctatg
     2881 ggagttatag taacaccctc taccttaaaa tctccgccca cctcgtttaa aataagcttt
     2941 ggctttccgc cagcagaata aataccgtca aaaatatatg caaaattatt cttgagattg
     3001 ttaactgtga gtgcgtttcc ataacatgga attgtcattt tgtttataaa attataggtc
     3061 ctcagatcgt ctattccatg tgtgtgatca gcatgagagt gggtgtacaa aacagcatga
     3121 attagcgtaa tatcatgcct tagggcctga aaccgcagat cgggtgaagt gtctattagt
     3181 aaattaatat tatttacttg aataaatacc gaacatcggg ttcttttatt tttaggattg
     3241 tcagacaagc aaacattaca actacaacct actattggta ctcccgttga agttgcgcaa
     3301 cctaggataa ttactttcat taaacaaaat ttagcattaa aatataattt ttaaaataat
     3361 tagtaacact ttttaatttt aatagttttt aaataaagtc ttttctttta gtaaaaaagt
     3421 atttataata ataatttaaa gtggttaggc aatatacttt aattaaatta ttaattattt
     3481 tcaccctttt ggctttatct gcccagagta gagctgcaga taaatcagaa gagctgtttg
     3541 ataacgccaa tagcttatat aagaccattt taaaagatac ttccaaagct caaaatgtag
     3601 aaatttggca aaaacttgcc gatgcttttt atggcatttt caaatcatat aacaatagcc
     3661 aaaaagcccc tactgcgctt tttatttcag gaaagctcta cgaaaagatg ggatataatt
     3721 tcaattcaaa ggattatctt aaaaaatcca tagatgtttc aagagagctt actatatttt
     3781 acccaaaaag taactacgca gatgacgcac agataaggat tgccaggatt actgagggtt
     3841 gggacaaaac tcaggctttt attgagtatg acaaagtgct caatgagttt gcgggcggcg
     3901 atatggttta tgccgctaca aagaaagtaa aacaactcga caagtacaag ccaaagggct
     3961 attcgtattt aaaaaataag agaaatacaa caattgtaag ccagagtgaa attaaaaaca
     4021 ccaagggcct ttcaaaagta actaatatca gaaatttctc acttagagat tacaccagag
     4081 ttgtcataga tatggaaggt caaaaacgat ataaatctca cctattaaaa ccagacccaa
     4141 aacatggcaa accaccaagg ctttttgttg atattgacgg aacctatatt gatcctaatc
     4201 taaagattcc atcactcgaa gccggccttt tggaacaaat caggtttgga agaaatacgg
     4261 acaataaagt aagggtagta c
//
LOCUS       DBNGSV010000236         5583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1080765, whole genome shotgun sequence.
ACCESSION   DBNGSV010000236 DBNGSV010000000
VERSION     DBNGSV010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1080765"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..697)
                     /gene="thpR"
                     /locus_tag="AC159U_08655"
     CDS             complement(161..697)
                     /gene="thpR"
                     /locus_tag="AC159U_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3337168.1"
                     /translation="MRIFIAAKLPDETIEHIEDYIDSISNRVSGVKWEKPEKLHITLK
                     FLGNIDDNQLSAVNDVISESVSNICRINMTVSHFGAFPGLSRLKILYVGFDKSQQITD
                     LQSSIDKGLATIGFEREAREFIPHVTIARVKSRCKINPPLPIIEQKASEIERIAVVKS
                     SLTSSGSEYQNLFVYELS"
     gene            complement(737..1993)
                     /locus_tag="AC159U_08660"
     CDS             complement(737..1993)
                     /locus_tag="AC159U_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337169.1"
                     /translation="MYQRTLILTLFLFLCSALNSFSKIEIDINTPTIQPIPIAIADLN
                     ALGQVNQNAREFVEVLKQDLKNSGLFAIVQAPMSPGSAVNYQAYFDAGADALIKGDYK
                     VTGTNIEIAIRLFDLKDEQPLVGRSYEVSPRRVSEAAHRFANHVLKELTGIDGFFTSK
                     VVYVSGSKRKRDLYLMDYNGENIRKLTSHNSLVLSPSCSKDGSKVVFNSDKVWDQDLY
                     VIDLYPRFKEKRISKPYKLDQSAEWSPDGSKIVFSLDGDIAIANANGTGVRKLTRGRA
                     IDVSPTWSPDGSRIAFVSDRAGSPNIYIMSASGKNVKRLTKSGYNTDPSWSPNPKINK
                     IAYVKVEKGGGVNIFTTNSDGSGEQRLTWASGRNENPSWTPDGHYISFVSNRAGSNNI
                     YLMFHNGQNPVRLSKGAPKMFPTWCG"
     gene            complement(2067..2663)
                     /locus_tag="AC159U_08665"
     CDS             complement(2067..2663)
                     /locus_tag="AC159U_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3337170.1"
                     /translation="MLASPVLVLNRYFVPITVTSLKRAFIMLYGGVAKAVAGNYETFD
                     FESWSEIAALKDEDSIRTVSRVIKAPRVIMVLRYDKMPRNETKFNRLNIFRRDGGKCQ
                     YCGKNFPKSEFTIDHVVPRSHGGKSIWENVVCACSRCNRKKGGRTPVQANMKLINRPK
                     KPNWDPFSNFYVRSVRYEEWKPFLSFVDASYWNVELSE"
     gene            2784..2936
                     /locus_tag="AC159U_08670"
     CDS             2784..2936
                     /locus_tag="AC159U_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337171.1"
                     /translation="MNDIFSVFMVQALLWIVLLFFLFRLIKTNDKLKKEINLLTEKLE
                     IENKEG"
     gene            2937..3704
                     /gene="lgt"
                     /locus_tag="AC159U_08675"
     CDS             2937..3704
                     /gene="lgt"
                     /locus_tag="AC159U_08675"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3337172.1"
                     /translation="MYPELLRIGNFVISSFGLMVAIAFLVGYWITSLEFKRKNMDQNL
                     LGNLLLVSMIGGIGGAKIMYLFENVPFSELMSNPLTHLFSRGGLTFYGGFIGSVLMIW
                     LLTVKSKVSFFKVLDTCAPALAIGHSIGRIGCFLVGDDYGIKSDVPWAVAFPKGLPPT
                     TDPVHPTQVYEVILLGLVFFALWKLRKKHRPDGWIFSMFLILAGIERFLIEFIRSTTP
                     SPIDGLSVAQLMAVGLIALGIIKIITLKNKPASQSTA"
     gene            3736..5286
                     /gene="lnt"
                     /locus_tag="AC159U_08680"
     CDS             3736..5286
                     /gene="lnt"
                     /locus_tag="AC159U_08680"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3337173.1"
                     /translation="MRDLNKKQTILSIISAVIIALTFIVDLAWPLAFISFIPLFICIE
                     NETPKKALKIGTLTGTIAYFITTYWLIGTITNFGGFPFILGILFHLIISVYSGLMFGL
                     FCLFISSELLKRFKYAATILIGIIWVATEYFYPLLFPFSIASPLANFHPIIQVSDLFG
                     VGFLSLVVIIFNHSLYKIYRNQKLNEKFPVTELSVSMVLVLAVLVYGYLKIESVTKIV
                     DESPKLRVGIVQGNFDFTEKKQNNYLAMVNKHKELSYELSDAQLIIWPETAIQIFIPT
                     TFKYLAYGDTQLIPFIKDKYFLTGGLSFDPPPPGGDMDNVNKYNSAFLTDARGQILGR
                     FNKIKLLLFGEYLPFSTYFPSIKKLSPATGDYVPGRELNLMIIDKLGVKIAPLICYED
                     IIPSFSRQFKEKGANILINLTNDAWFGKTIAAHQHLLLSVPRAVENRTYLIRATNTGV
                     SAIIDPLGKVVIKSDIFERLTLTGEVRLMNNESTFYVKYGHYFYIICFIITLIYISVL
                     GYRKRALK"
BASE COUNT         1675 a         1094 c          971 g         1843 t
ORIGIN      
        1 atcacatcac cgggcttaac aacatactgt tttccgcctg ttctaattac tgcctgcatc
       61 tactgggtcc tccaaattga atgaacatat aaaataaact gtgcaatata ccctactatc
      121 tcaaatattt aaacatatta tttataatct actgagaaaa tcaacttaat tcatatacaa
      181 atagattttg atactctgat ccagaagagg ttaagctgct ttttactact gctatccttt
      241 ctatttctga ggctttttgt tcgattattg gtaacggagg attaatttta caccttgatt
      301 ttactcttgc tatagtaaca tgagggataa actctcttgc ttccctttca aacccaatag
      361 tggctaatcc cttatcaata gagctttgca gatcggttat ctgctgagat ttatcaaacc
      421 ccacatataa tatttttagt ctcgatagac ccggaaaagc tccaaaatga gaaactgtca
      481 tgtttatccg gcaaatattg gaaactgatt cagaaattac atcattaact gctgagagtt
      541 ggttatcatc aatattgcca agaaatttaa gggttatgtg caatttctcc ggtttttccc
      601 attttacacc agagactcta ttcgaaatag aatctatgta gtcttcaata tgttctattg
      661 tttcatccgg cagctttgcc gctataaaaa ttctcaagat attattttat tagccagtgc
      721 ataaatccaa cagcgactat ccgcaccagg ttggaaacat ctttggtgcg cccttactaa
      781 gtcttaccgg attttggccg ttgtgaaaca ttaaataaat attgtttgag cctgctctgt
      841 tggaaacaaa agagatgtaa tgaccatccg gagtccagga tgggttttca tttcttcccg
      901 atgcccaggt tagacgctgt tcccctgatc catcagaatt tgtggtaaat atattaaccc
      961 ctcccccttt ttctactttt acgtaagcaa ttttgttaat tttcgggttt ggagaccatg
     1021 aaggatccgt attgtagccg ctctttgtaa gccttttcac atttttccct gaggcactca
     1081 tgatatatat attgggcgat cctgccctgt ctgatacgaa tgcaatcctt gaaccgtcgg
     1141 gagaccatgt aggtgaaaca tcaattgctc tgcctcttgt aagttttcgc acacctgttc
     1201 catttgcatt tgctatagca atatctccat cgagactaaa aacaattttg ctgccatcgg
     1261 gtgaccattc cgcgctttga tcgagcttat atggttttga tattcttttt tctttaaacc
     1321 tcggataaag atcgataaca tataaatcct gatcccagac tttatcggaa ttaaaaacta
     1381 cttttgatcc gtccttcgaa cagcttgggg agagaacaag agaattgtgg cttgtaagct
     1441 ttcttatgtt ttcaccatta tagtccatca ggtaaagatc tctttttctt ttagaacctg
     1501 aaacataaac tacttttgat gtaaagaaac cgtctattcc cgtaagttcc tttaacacgt
     1561 ggttagcaaa cctgtgtgca gcttctgaaa ctctgcgggg gcttacttca taggacctcc
     1621 caaccagagg ctgctcatcc tttaaatcaa acagccttat ggcaatctcg atattagtgc
     1681 ctgttacctt gtagtcacct ttaataagcg catcagcgcc ggcatcaaaa taggcctgat
     1741 aattcacagc actgccgggc gacataggag cctgtacaat agcaaataga cctgagtttt
     1801 taaggtcctg tttgagtact tctacaaact ctcttgcgtt ttgatttacc tgccccagag
     1861 cattcaaatc agcaatggct ataggaatgg gttgtatggt tggagtattg atatcaatct
     1921 caatttttga aaaagaattc aatgcactgc atagaaagag gaataaagtt aatattaatg
     1981 ttctttgata catatacggt gtctcctata attacttaat aagtatataa gaaatataaa
     2041 tcaaataatc aaataaaggt tacaaactat tcagaaagct caacattcca gtaagaagcg
     2101 tccacaaaac taaggaaagg tttccactct tcgtatctta ccgatctaac ataaaaatta
     2161 gaaaatggat cccagtttgg cttctttggt ctgtttatca gtttcatgtt tgcttgtaca
     2221 ggtgtcctgc cgcctttttt tctgttacac ctggaacagg cacataccac attttcccag
     2281 atactctttc caccatggga gcgtggaaca acatggtcaa tagtgaattc cgatttggga
     2341 aagtttttgc cgcaatactg acactttcca ccatcccttc tgaatatgtt aagcctatta
     2401 aactttgttt cgttccttgg cattttgtca tagcgcagca ccatgataac ccttggggct
     2461 ttaataaccc tgcttacagt cctaatggaa tcttcatcct taagagctgc aatctcggac
     2521 catgattcga aatcaaaggt ctcgtagtta cccgcaaccg cctttgcaac tcctccgtag
     2581 agcattatga atgccctttt taggcttgtt accgtgatgg gaacaaaata tctgtttaat
     2641 accagtacag gacttgctaa catattaacc tatgagatta cctttttttg attacagcat
     2701 caagaaacat caatttacta taattattta gcttatataa aacattcagt taaatttatg
     2761 tgataacttt taaggagaag taaatgaatg atatattttc ggtgttcatg gttcaggctc
     2821 tgctgtggat tgtgttatta tttttcttgt tcagactcat taaaacaaac gataaactta
     2881 aaaaagaaat taatcttcta actgaaaaac tagaaataga aaataaagag ggataaatgt
     2941 atccagaact tctaagaata ggaaactttg taatatcatc ttttggactc atggtagcga
     3001 tcgcgtttct ggttggctac tggatcactt cactcgaatt caagagaaag aatatggacc
     3061 aaaacctttt gggaaatctg cttttggtat cgatgatagg aggtattggc ggggcaaaaa
     3121 taatgtattt atttgaaaat gtgcccttct cagagcttat gagcaatccc ctgacacacc
     3181 ttttttcccg cggcggtctc actttttatg gtggttttat aggctcggtt ttgatgatct
     3241 ggttattaac agttaaaagc aaagtgagtt tttttaaggt actggacact tgcgctccgg
     3301 cacttgcaat cggacattca attggcagga ttggttgttt tcttgtggga gacgactatg
     3361 gaattaaatc agatgtgcca tgggcagtag catttcccaa gggtcttcct ccaaccacag
     3421 accccgttca ccctacccag gtatatgaag tcattctttt aggactagta ttctttgctc
     3481 tttggaaact tagaaaaaag cacagacctg atgggtggat tttctccatg tttcttatat
     3541 tagccggaat agaaaggttt ttaattgaat ttataaggag cactacccca agcccgatag
     3601 acggtctttc cgtagctcag cttatggcag taggacttat agcgcttggt ataataaaaa
     3661 taattacatt aaaaaacaaa cctgcttctc aaagtaccgc ttaagatcat aacaaagtga
     3721 gatcagaccc ccaatatgcg cgatcttaat aaaaagcaga caatactttc aataatctca
     3781 gctgttatta tagcgcttac ttttatagtt gatcttgcct ggccacttgc gtttatttca
     3841 ttcataccgc tttttatctg tatagagaat gaaacaccaa aaaaagcatt aaagatcggc
     3901 acactaacag gaacgattgc ttattttatt accacctact ggcttattgg tacaattaca
     3961 aattttggcg gttttccatt tatattaggt attttatttc atcttattat aagtgtctac
     4021 tccgggctta tgtttggtct attctgtctc tttattagtt cagaactgct aaaaagattc
     4081 aagtatgcag ccaccatact gattggaatc atctgggttg ccaccgaata tttttatcct
     4141 ctgcttttcc cgttcagcat agctagtccg cttgcaaact ttcatcccat catacaggta
     4201 tcagacctct ttggagttgg gtttttgagc cttgtagtaa taattttcaa tcactcctta
     4261 tataaaattt atagaaatca aaagctcaat gaaaagtttc cagtcacaga gctgtctgtt
     4321 tcaatggtcc tggtacttgc tgtattagta tatggctacc taaaaattga atctgttaca
     4381 aagatagtgg atgaatcgcc aaaattaaga gtaggcatag ttcagggaaa ctttgatttt
     4441 acagaaaaaa agcagaacaa ctatcttgct atggttaaca agcacaaaga gctttcatat
     4501 gaattatcag atgctcagtt aataatctgg cctgaaactg caatacagat atttattcct
     4561 accacattta agtacctggc atacggagac actcagctga ttccttttat taaagacaaa
     4621 tattttctca ccggcggcct gtcatttgac ccacctccac ccggaggtga tatggataat
     4681 gtaaataaat acaactccgc atttcttact gatgccaggg ggcagattct tggaaggttt
     4741 aataaaatca agcttctttt gtttggcgaa tacctgccgt tttcaactta tttcccgtca
     4801 atcaaaaagc taagtcccgc tacaggtgat tatgtacctg gaagagaatt gaatttaatg
     4861 ataatcgata agcttggagt aaagattgcc ccgcttattt gctatgaaga cattatcccg
     4921 tctttttcga gacaatttaa agagaaagga gccaatattc tcattaatct taccaatgat
     4981 gcatggtttg gaaaaactat agcagcacac cagcatttgt tactatctgt tccaagagcc
     5041 gttgaaaaca ggacttatct aattagagca acaaatactg gagtcagtgc tattatagac
     5101 cctcttggca aagttgtaat aaaatctgac atattcgaaa ggctaaccct aacgggtgaa
     5161 gtgaggttaa tgaataatga aagcactttt tatgtgaaat acggacatta tttttatatt
     5221 atctgtttta ttattacact aatttatata tcggttctag gctataggaa acgagcttta
     5281 aaataagatt accttactta atatcttctc aatcttttta ttaatccata aaatagttgc
     5341 tattaatcgt actattctgc cctaatttat agatttacta ccaattgtct taaataaggt
     5401 agtatttggt taatctggca taaatattgc ttatttaata ttacatacaa atttttatag
     5461 gagctcgcat agaacagagc tataagtaat gcagaatctc ctttatttta ttaactaaat
     5521 ctacatttct gttattaaat ctaaattcca tctcttttag atacaaaaca aagtgttttc
     5581 tgt
//
LOCUS       DBNGSV010000237         3966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_527361, whole genome shotgun sequence.
ACCESSION   DBNGSV010000237 DBNGSV010000000
VERSION     DBNGSV010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_527361"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC159U_08685"
     CDS             complement(<1..572)
                     /locus_tag="AC159U_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010900157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR3337174.1"
                     /translation="MQEQILGLLNQISGYVWGPVLIILLLGTGIYLTIILRGIQFRAL
                     VPSLLLAFTKGKQDEKSQGDISHFQALMTALSATVGVGNIAGVATAIALGGPGALFWM
                     WITGLVGMATKYSEAVLAVKYREQDKLGTMSGGPMYYISKGLGMKWLGSLFAIFGAIA
                     AFGIGNMVQSNSVAHALESAFGITPWITGIV"
     gene            complement(721..1011)
                     /locus_tag="AC159U_08690"
     CDS             complement(721..1011)
                     /locus_tag="AC159U_08690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3337175.1"
                     /translation="MAVAEKRLYQRLDIKEKIKFGEDTPVHDGFTRNLSPDGMSIVSE
                     YGLDANSNIKVKILRSSGQSIDLEGKVVWSSNPPGVNKLIGIKFRSSNEDLL"
     gene            1096..2034
                     /locus_tag="AC159U_08695"
     CDS             1096..2034
                     /locus_tag="AC159U_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337176.1"
                     /translation="MKRHNILTLFILAIFSSNSYGQVCSSWSEARVVGKLDHTMINEA
                     SGIEVSLIDNSILYHINDSGNGPYYYSSKFDGSESKKIRINNLNLKRSDYEDISTGKC
                     YDKKCLFIGDIGDNLKSRDNINIIIIEENNKSSSVTPLKIVRLIYPDHPRNAEAFAVH
                     PNGDLFIITKEESLRRQKSFPSKIYKTSRNKWENAGPKPLTLEYVGTLDIKPINSKTN
                     GFLDNVITSLDISEDGKKFVVLTYQHAYEFNIDLSKAHSSSFDNFNAEKDYRVIELKR
                     LPQQESIAYLQGSKSILYNTEYRAIAPNLMRVDCLD"
     gene            complement(2038..2568)
                     /locus_tag="AC159U_08700"
     CDS             complement(2038..2568)
                     /locus_tag="AC159U_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337177.1"
                     /translation="MLFSSSIKIISFLIFFFSLSLTGIKQTSAGDLPPPETIGACCFE
                     VGEGVCQQISLDECLSAETGIGWSDEIECELDTCIPDTSGAADCCTVHSSTACETSSC
                     SETICEIDPYCCIFDWDEICVEEAFELCPQVCTSSGDPIVIVPTINQLGIIFLVVLMG
                     VYSVFRLILIDNKYKC"
     gene            complement(2573..3778)
                     /locus_tag="AC159U_08705"
     CDS             complement(2573..3778)
                     /locus_tag="AC159U_08705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3337178.1"
                     /translation="MIGTASSLFDNELDNVLIVDDDKDFCDALADILESKGYPFLTAT
                     NALQAQEITQNQQVQVIFVDVRLGSTNGLDLIKSFKRSNSNSICVVMTAYTDFSTAVD
                     ALREGAYDYLQKPFHPNEFDAVLQRCMNIVKLEREKKRAQEDLRIANLVLETRIEERT
                     SELHEINQLLKKEIAQKNETEQELKAALKEKDILLKEIHHRVKNNLQIITSLLSLQSD
                     TLDDSYVKNKFEQCETRIHAMSMIHENLYNSRSLASIDLGKYVEAVAENLFSIFGIRR
                     DEIDLNIDINDVSLDFNTAIPCGLLINELITNSLKYAFRSGHKSVGDNKSAKIIRVVA
                     RIEDDNEYVLSVCDNGVGIPDDIDFLKTSTLGLQLVNALTQQLHGSISLDRSNGTEFI
                     IKFRTDNDN"
     gene            complement(3854..3958)
                     /locus_tag="AC159U_08710"
     CDS             complement(3854..3958)
                     /locus_tag="AC159U_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3337179.1"
                     /translation="MTVVKQIMEEHKGQVEISSEEGKGTEVLIWFPLG"
BASE COUNT         1317 a          771 c          650 g         1228 t
ORIGIN      
        1 actatcccgg ttatccatgg ggttattcca aaagcggatt caagtgcatg cgcaacagag
       61 ttagactgaa ccatgttgcc tataccaaaa gcagcaatag ctccgaatat agcaaataaa
      121 gaccccagcc acttcatacc aagccccttg gatatgtaat acatcggtcc gccgctcata
      181 gtgcccaact tatcctgttc tctgtacttt acagcaagta cggcttcaga atactttgtt
      241 gccattccaa caagaccagt aatccacatc caaaacagtg ccccgggacc tccaagagca
      301 attgcagtgg ctacccctgc aatattacca acacccacag tagcagaaag agcagtcata
      361 agagcctgga agtgagaaat gtcaccttga gatttttcat cctgtttccc ttttgtaaat
      421 gcgagcagaa gagatggaac aagagctcta aactgaatgc ctcttaatat aatcgtaaga
      481 taaataccgg ttcccaataa cagtatgatt aaaaccggtc cccatacata gccactgatt
      541 tgatttaata agcctaaaat ttgctcttgc atatatttgt ccttttatga atataaatgg
      601 gattgaatac aataattaat tagatcaata ttaactataa acaagtcata aaaacaagag
      661 atatatgaaa atctaaatcc agtgtttata aatgtactat ctcgatctaa atctatatac
      721 ttataacaaa tcttcattag aacttctgaa ttttatacct atcaatttat ttacaccagg
      781 gggattagat gaccagacaa ccttaccttc aagatcaatt gattgccctg aagatctaag
      841 aattttaact tttatatttg aattagcatc caggccatac tccgaaacaa tagacattcc
      901 atcgggcgag aggttgcggg taaatccgtc atgaacagga gtatcctctc caaacttgat
      961 cttctctttt atgtccaacc gttgatataa tcttttctca gcaactgcca taagagtgat
     1021 ataataatga ttttttaaga tatgacaatt aatatttgtt gttatatctt ttatcttgat
     1081 atactagtta tgcatatgaa aagacataac atattaactt tatttatact cgcaatattt
     1141 tcttcaaatt cttatggaca ggtatgcagt tcatggagcg aagcaagggt tgttggcaaa
     1201 cttgatcaca caatgataaa tgaggcaagc ggtattgaag tatctttgat agataatagc
     1261 attttatatc atatcaatga ctccgggaac gggccatatt attattcgag caaatttgat
     1321 ggctctgaat caaagaaaat tcgaataaac aatttaaatc tgaaaagatc tgactatgag
     1381 gatattagca ccggtaaatg ttatgataaa aaatgtttat tcattggtga tataggtgat
     1441 aatctcaagt caagagacaa tataaacata attataatag aagaaaataa taaaagctct
     1501 tcggttactc ctttaaaaat agtaaggctt atatacccgg accatccacg caatgcggaa
     1561 gcatttgccg tacatccaaa tggggatctg ttcattataa ctaaagaaga aagtcttagg
     1621 cgacaaaaat catttccatc caaaatatat aaaacaagca gaaacaaatg ggaaaatgcc
     1681 gggccaaaac cgcttacact agagtatgtt ggaacattag atattaaacc aattaattct
     1741 aagacaaacg gttttcttga taacgttatt acatcattag atatatctga agacggcaag
     1801 aaatttgtag tattaacata tcagcatgcg tatgaattca atatagattt atcaaaagcg
     1861 catagcagta gttttgataa ttttaatgca gaaaaggatt atagggttat cgaattaaaa
     1921 agacttccac aacaagaaag catcgcatat cttcagggct ctaaaagtat tttgtataat
     1981 actgaatata gggcgattgc tccaaacctc atgagagtag attgtttgga ttaattatta
     2041 gcacttatat ttgttatcaa taagtatcaa tctaaaaaca gagtatacac ccattaacac
     2101 gaccaaaaat ataattccca attgattgat cgttggaaca atgactatcg gatctccact
     2161 tgaagtacag acctgtggac aaagctcaaa tgcttcttca acgcatatct catcccagtc
     2221 aaatatacag cagtaaggat caatttcaca aatagtctct gaacacgaag aagtctcaca
     2281 ggcagtgctg gaatgaaccg tacaacagtc tgctgcacca gatgtatcgg gtatgcaggt
     2341 atcaagctca cattcaattt catcagacca gccaatgcca gtttcagcag ataaacattc
     2401 atcaagactt atttgttgac acacgccttc accaacctca aaacaacatg cccctatggt
     2461 ttcaggtggg ggcagatcac cggcagatgt ttgtttaata ccggtcaaag aaagggaaaa
     2521 gaagaatatc aagaagctta taattttaat agaggaacta aataacattg atctaattat
     2581 cattatcagt tctaaacttt ataataaact ctgtcccatt tgaacgatca aggctgatac
     2641 tgccatgaag ctgttgggta agggcattaa caagctgcag acccagagtg gaagttttta
     2701 gaaaatctat atcatctggt atccctacac cgttgtcaca gacactcagc acatattcat
     2761 tgtcatcttc aattctggca actaccctta ttatttttgc agatttattg tctccgactg
     2821 atttatgtcc agagcgaaaa gcatatttaa gtgagtttgt aataagctca tttatgagca
     2881 gaccacatgg tatggcagta ttaaagtcta gtgagacatc atttatatct atattcaaat
     2941 caatctcatc cctgcgtatt ccaaaaatag aaaaaaggtt ttcagcaact gcctctacat
     3001 attttcccag gtcaatagat gcgagacttc tagagttata caggttttca tgaatcatgg
     3061 acattgcatg aattctggtt tcgcattgct caaacttatt tttaacatag ctatcatcaa
     3121 gagtgtcaga ctgcaaagat aacaagctag taattatctg taaattattt ttaaccctgt
     3181 gatgaatctc ttttaataaa atatcttttt ctttaagagc agcttttaac tcttgttcag
     3241 tttcgttctt ttgagctatc tcctttttta gcagttgatt tatttcatga agctcagacg
     3301 ttctctcctc aattcttgtt tcaagcacca ggtttgctat tctcagatct tcctgtgctc
     3361 ttttcttttc tctttcaagt tttacgatgt tcatacatct ttgcagtacg gcatcaaact
     3421 catttgggtg aaaaggcttt tgcagataat catatgcccc ttccctaagc gcatctacag
     3481 cagtagaaaa atctgtatag gccgtcatta ctacacaaat ggaattggaa tttgatcttt
     3541 tgaatgactt aatgagatca aggccatttg tacttcctag tcttacatca acaaaaatca
     3601 cctgcacttg ttgattttgt gttatttctt gagcctgaag ggcatttgtt gctgttagaa
     3661 agggatatcc tttagattcg agaatatctg caagcgcatc gcagaaatcc ttatcgtcat
     3721 caactattaa aacattatct aattcgttat cgaataaact gctggctgtg cctatcatcg
     3781 tgcaatattt taactccaat atatatgcta aacaagctat tgtttaatat tcatatagta
     3841 agcagaaatt ctcttatccc aaaggaaacc atattaggac ttcagtgcct ttaccctctt
     3901 cacttgatat ctcaacctgt cctttatgct cttccattat ctgcttaact actgtcagac
     3961 caagtc
//
LOCUS       DBNGSV010000238        12706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_943153, whole genome shotgun sequence.
ACCESSION   DBNGSV010000238 DBNGSV010000000
VERSION     DBNGSV010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_943153"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC159U_08715"
     CDS             <1..197
                     /locus_tag="AC159U_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR3337180.1"
                     /translation="ILTPREEEIIRLRFGIDKESTYTLDEIGKRFDLTRERIRQIEKR
                     ALEKIADSDMGPLLSSFLQN"
     gene            314..835
                     /gene="cysC"
                     /locus_tag="AC159U_08720"
     CDS             314..835
                     /gene="cysC"
                     /locus_tag="AC159U_08720"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3337181.1"
                     /translation="MSGFTVWFTGLPSSGKSTLAKVLRGEIEKKDKHVEILDGDEVRL
                     RLSKGLGFSKEDRDENIRRISYVAKVITRCGAVAITCAISPYKDIRLEARNEIENFVE
                     VFVDCDVEECIRRDVKGLYKKALSGEIKNFTGISDPYEKPESPEITVNTSTETIEQSV
                     KKIVAGLKELGYL"
     gene            968..1399
                     /locus_tag="AC159U_08725"
     CDS             968..1399
                     /locus_tag="AC159U_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3337182.1"
                     /translation="MQVSKTLDYAVRSLTYMGQKPGNTLNMKEIADSQHIPLNYLAKI
                     MRRLVERDILKSMVGPDGGYVLRRQPSDITLKEIYEAIEGEFRIIDCMDREKICFFHE
                     QCTQLPVWDKIKISMLNILESTSLYDIIHSTEHRGSNQEAY"
     gene            1399..2835
                     /gene="sufB"
                     /locus_tag="AC159U_08730"
     CDS             1399..2835
                     /gene="sufB"
                     /locus_tag="AC159U_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3337183.1"
                     /translation="MSVDIEQLAEQEYKYGFVTDIEQDIAPKGLNEDTIRMISLKKNE
                     PQWLLDWRLEAYRKWLKMEEPKWPNVKYPKIDFNDISYYAAPKVKEKPKSLDEIDPEL
                     RETYDKLGIPLEEQMMLSGVAVDAVFDSVSLITTFKDKLAEMGVIFCSISEAVHEHPD
                     LVKKYLGYVVPVNDNFYAALNSAVFTDGSFVYIPKGVKCPMELSTYFRINAENTGQFE
                     RTLIIAEPGSYVSYLEGCTAPQRDENQLHAAIVELVAHDDATIKYSTIQNWYPGDKDG
                     KGGIYNFVTKRGRCIGANSRISWTQVETGSAITWKYPSCILEGDNSVGEFYSVALTNR
                     HQQADTGTKMIHIGKNTTSTIVSKGISAGLGQNTYRGLVQIGKNAENSRNYTQCDSML
                     IGDKCGAHTFPYIEVNNSTAHMEHEASTSKIGEDQIFYCQQRGLSAEDAVSLIVNGFC
                     KEVFKELPFEFAVEAERLLSVSLEGSVG"
     gene            2885..3640
                     /gene="sufC"
                     /locus_tag="AC159U_08735"
     CDS             2885..3640
                     /gene="sufC"
                     /locus_tag="AC159U_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR3337184.1"
                     /translation="MLEIKDLHVKIEDKEILKGLNLTVNPGEVHSIMGPNGSGKSTLS
                     QVLAGKESYEVTSGSITFKGKDLLELAPEERACEGVFMAFQYPVEIAGVANTYLLREA
                     LNSKRKYQGLEPLKHVEFIKNIKEKMKNLKIDDTMLSRSVNEGFSGGEKKRNEIFQMQ
                     VLEPDLCILDETDSGLDIDALQIVADGVNAMRDEGRSFIVITHYQRLLNYIVPDFVHV
                     LIDGKIERSGDKSLALELEDKGYTGIAQQPSAA"
     gene            3672..5012
                     /gene="sufD"
                     /locus_tag="AC159U_08740"
     CDS             3672..5012
                     /gene="sufD"
                     /locus_tag="AC159U_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3337185.1"
                     /translation="MQIILKDSTDSYLQGYKQLITSDTSSAKFAESTREEAINNFVKL
                     GFPTLRDEDWRFTNIVPIVKSQYSISTDVTTKVENIDIKKYLIHGLDSHLMVFVDGVY
                     SAELSNINDTDGKIIIKPISNALSDNRDIVEKHLFSCADIKQEAFTALNTAYFTDGAF
                     VYVPGNTELDKTVEVLYISADPVSSMNSPRNLIVVEKSSKVQVIEHYVSLTDSVYFSN
                     TVSEVIIGENSTVDHYLIEQDSKKAYNISSLEVKQSANSNLNSHSFLLGGAIVRNNIH
                     PVLGGQGCNSNINGLFLPSGRQHIDNFMKVEHASPHCDSRQVYNGILADKSKGVFHGR
                     IIVHEDAQKTDAKQTNRNLLLSDTAKIDTKPQLEIYADDVKCTHGATIGQMDKDSLFY
                     LRSRGIEQKLAKAIMLRAFASQNIEDIGDKVIKSYVEKLVKNWFSEIGMEAELD"
     gene            5038..5424
                     /locus_tag="AC159U_08745"
     CDS             5038..5424
                     /locus_tag="AC159U_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR3337186.1"
                     /translation="MNVDQMEFVLDHYKNPRNSGQIADADYSYEEGNPSCGDKIRIDI
                     KVADNKIAEARFSGKGCAISQASASILTEMIEGMELDAIKEMDGQEFLETLGVNISPI
                     RYKCALLALKVLKTSVFGDGVFEYEH"
     gene            5434..5745
                     /locus_tag="AC159U_08750"
     CDS             5434..5745
                     /locus_tag="AC159U_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3337187.1"
                     /translation="MGEFIKVAKISDVPAGTAKSFLVENEVIAVFNLNNNYYALRDQC
                     SHMDLPISDGIIEQSRVTCAYHGAEFDIKTGKALCAPAYDPVESFEVMVQDEQIYISI
                     D"
     gene            5772..7001
                     /locus_tag="AC159U_08755"
     CDS             5772..7001
                     /locus_tag="AC159U_08755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3337188.1"
                     /translation="MTKFDPYEIRKDFPILNRKVNGNPLVYLDNAATSQKPSQVIDCI
                     KSYYENNNSNIHRGVHTLSYESTVMYEDAHKKVAAFIGAEDWREIIFTRNATESLNLV
                     AYSWGLHNLNKGDEVIISIMEHHSNIVPWQMLAKVKGIVLKFIDVDDKGNLDLESFDN
                     LLSDKTRVVSVIHVSNVLGVVNPVEYISKKAKDHGALFIVDAAQSVPHMKLSVHELDC
                     DFLAASGHKMLGPTGIGFLYGKKDLLENMEPFFYGGDMISTVTKTSSTWNELPWKYEA
                     GTPNIADGIALGAAIEYLEKLGLKNIEQYESELLDYTLDRLKDIPWIDIYTPLTGKRV
                     GVISFNVNGVHPHDVAGIMDEDGIAVRSGHHCTQPLMTRLSIENALRASFYIYNTNEE
                     VDKFISSLLRAYDIFKK"
     gene            complement(7004..7864)
                     /locus_tag="AC159U_08760"
     CDS             complement(7004..7864)
                     /locus_tag="AC159U_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR3337189.1"
                     /translation="MSAGEKMFGTAPNINFWILIEYTRQWEEKAFNNCSISKELKDVI
                     RNLAGMHPKSRIQLIRRNSKSSKLKLYIAITKSSEKEVFEFSLDSYDQILTIDFEKAL
                     KNSDHISAKPLLLVCTHGSYDKCCGTMGKQLYNNLFSIEKDFEVWQTTHLGGHRLASN
                     ILLLPEGIYYGRVDEDNYYKIKECYLKNSLCTELLRGRCFYSSDEQAAEYFLAMENND
                     TKISDFRLEQVSRTDSGQINTVFTNNHNGYVYNVLLDKKDNAVQVLASCKDKHKKFFP
                     QYELINITRE"
     gene            8164..8628
                     /locus_tag="AC159U_08765"
     CDS             8164..8628
                     /locus_tag="AC159U_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3337190.1"
                     /translation="MYKTSADFLNEMEHVDMVMIYVVDEDTNQAVLEAHRNLTADYIA
                     RASRVDRPKGITWRVIQTNQFLNVKDAQTDESIGPAGKALGKHGVLGMPITLTTGGEA
                     KGVIWLWSDTERAFTDEEVKLLSAIGNQIATAIAWAKQYEDRRKVWQERFKV"
     gene            8671..9297
                     /locus_tag="AC159U_08770"
     CDS             8671..9297
                     /locus_tag="AC159U_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3337191.1"
                     /translation="MSKITIFVADDHPIVKEGLITILNTQSDFKVVGEAMDGTKVVSG
                     VEETNPDILVLDLEMPGMDGIEIIEKLNKSGCKAKAIIFTAYKTDDRIFSAFELGAKG
                     YLLKGSPKEEIFDAVRNVYNGGTSVHPDVTDRLVKGVNQKLDKLTPRELDVMKSLSRG
                     LTNKEIAYELFISERTVKFHISSIFTKLGAETRTEALSIAIQKGLLSI"
     gene            complement(9290..9700)
                     /locus_tag="AC159U_08775"
     CDS             complement(9290..9700)
                     /locus_tag="AC159U_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3337192.1"
                     /translation="MNTSTIKPEKSKTILILEDEMIIAKSIKSYIEKIGYSVAGIAQN
                     GSDAIQIAEQYKPDLAILDVVVDGDMDGIETGRELLKLNVPFLYLTAYGDTKTRQRAK
                     ETKPLGYIIKPFNDTVILNTIKEAFEQIESSKSK"
     gene            10014..10841
                     /locus_tag="AC159U_08780"
                     /pseudo
     CDS             10014..10841
                     /locus_tag="AC159U_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
     gene            10842..11348
                     /locus_tag="AC159U_08785"
     CDS             10842..11348
                     /locus_tag="AC159U_08785"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR3337193.1"
                     /translation="MPPLRERKEDIKNLCMHFVDLFNRKLGTKVDSLSDDVVSVLENY
                     DWPGNIRELENVIKYAVIKADDIITLNDIPNHMDKNGAAQIAESVNELISCTTNNSDN
                     NSVDIKLKIQVLDTMDLKQVAKDVSDIVKRAIISKVSKTSKLSQTEITKNLNIDPKTY
                     RTIIKKLQ"
     gene            11492..12619
                     /locus_tag="AC159U_08790"
     CDS             11492..12619
                     /locus_tag="AC159U_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3337194.1"
                     /translation="MKLENILIVEDELIVAKHIEKQLIKCGYEVTAIVNNGSSALNEV
                     KSNPPDLILMDVNIEGDLDGIQTSALINKQYKIPIVYLTAFTEKNTIERAKETEPYGY
                     IIKPFSGKNLETTVEIAYHKFQIEKKLRDSESWLINTMSNLADAVITTEPNGHIKYLN
                     PVAESFTGWTNEQAVGKNLEEILVLYDETEYKPVGDVLEHILSAKNENDNYAFLKSKQ
                     GKDIIIRFKAAHIKQDNKDNIGVVIVFQDFTDHRLQELRLLKSHERLQIKVEKNIKEI
                     SSISADLKSSKEKYKTFIENVFDLIVEVDSDGIFSYVNNNYKKLFGHTESELSGKKIF
                     DFIYSEDLEEFENIFMDSIQNNTDFSHSYRSINKSGDLVWL"
BASE COUNT         4402 a         2003 c         2620 g         3681 t
ORIGIN      
        1 ctatacttac tccaagagaa gaagagataa taagactgcg ttttggtata gacaaggaaa
       61 gcacatatac tcttgatgaa ataggtaaaa ggtttgacct tacaagggaa aggatcaggc
      121 agattgagaa aagagcttta gaaaaaatag cagattctga tatgggccca ttattaagca
      181 gttttctaca aaactaatac tttatagatt ataagtaata tctattattt atcacttgtt
      241 taaatttctt tattccgata aggtgtactc ttagttctaa gataaatcaa ataatcgtgg
      301 aggatattaa tacatgtctg gatttactgt atggttcact gggcttccaa gttcgggtaa
      361 atcaactctt gcaaaagtgc taagaggcga gattgaaaaa aaagataaac atgtagaaat
      421 attagatggt gatgaagtta ggctgaggct ttctaaaggg cttggatttt ctaaagaaga
      481 cagggatgaa aatattagaa gaatttctta tgtagctaaa gtgattacta ggtgcggggc
      541 tgttgctatt acttgtgcaa tttctcctta taaggatata aggcttgaag ccagaaacga
      601 aatagaaaat tttgttgaag tatttgtaga ttgtgatgta gaagaatgta taagaagaga
      661 tgtaaaggga ttatataaga aagcactgag tggggaaata aaaaacttta ctggaatctc
      721 agatccatat gaaaaaccgg aaagtcctga gattaccgtc aatacctcaa ccgagaccat
      781 tgaacaaagc gtaaaaaaga ttgttgccgg cctaaaagaa ctgggatatt tataaaaaat
      841 ttttacagtt ccagccttga ctttaaagct tatcggggca tcttatatat tatagacata
      901 cataatccat aataggatta tattaagagt tcaattgagc ttaatagggg taatatagga
      961 aaagaaaatt caggttagca aaacattaga ctatgccgtg aggtctctta cttatatggg
     1021 ccaaaaaccg ggtaatacgc ttaatatgaa ggaaatcgcc gactctcagc atattcccct
     1081 aaactatctg gctaaaatca tgagaaggct tgtagaaaga gatatactca aatccatggt
     1141 tggtcctgat gggggttatg tgcttagaag acagccttca gacataactc ttaaagaaat
     1201 atatgaagcc attgagggtg aatttaggat tatagactgt atggatagag aaaagatatg
     1261 cttttttcac gaacagtgca cacagctgcc tgtttgggac aagatcaaaa tatccatgct
     1321 caacatatta gagagcacat cactttacga tattattcat agtacagaac atagaggcag
     1381 taatcaggag gcttattaat gagtgttgat atagagcagt tagccgagca ggaatataaa
     1441 tatggctttg ttacagatat tgagcaggac attgctccaa aggggcttaa tgaagacacc
     1501 attaggatga tatctttaaa aaagaatgaa cctcagtggc ttcttgactg gagactcgag
     1561 gcttatagaa aatggctcaa aatggaagag ccgaaatggc cgaatgtaaa gtatcccaaa
     1621 attgacttta acgacattag ctactatgca gctccaaagg taaaagaaaa acccaagagc
     1681 cttgatgaaa ttgatccgga gcttagagaa acctatgaca aacttggcat cccgcttgaa
     1741 gagcagatga tgctctcagg tgtagccgta gatgccgtat ttgacagcgt ttcacttatc
     1801 acgactttta aggataaact tgctgaaatg ggtgtaattt tttgctcaat ctcagaagct
     1861 gttcatgaac atccggatct tgtaaaaaaa tatctgggat atgtagttcc cgtaaatgat
     1921 aatttttatg cggctctaaa ttcagcagta tttacagacg gctcatttgt ttatattcca
     1981 aaaggcgtta aatgtcctat ggagctttct acgtacttta ggataaatgc tgaaaacaca
     2041 gggcagtttg aaaggacgct tataatagct gagcccggaa gctatgtaag ttacttggaa
     2101 ggctgtacag ctccacaaag ggatgagaat cagctccatg cagcaattgt tgagcttgtt
     2161 gcgcatgatg atgctacgat taagtattca acgatacaga actggtaccc aggagacaaa
     2221 gatggtaagg gcggcattta taattttgtg actaaaagag gaaggtgtat tggagcaaac
     2281 tccagaattt cgtggactca ggttgaaaca ggatcagcaa tcacatggaa atatccaagc
     2341 tgtattttag agggagataa ctcagtaggc gagttttact cagtagcact tacaaacaga
     2401 caccagcagg ctgataccgg aacgaaaatg attcacattg gtaaaaatac tacaagcaca
     2461 atagtatcaa aaggaatctc ggccgggctt ggtcaaaata catacagagg acttgtgcag
     2521 attggaaaga atgcagaaaa ttccagaaac tatacccagt gcgattccat gcttattggc
     2581 gataaatgcg gcgctcatac ttttccctat attgaagtaa ataattcaac agctcatatg
     2641 gagcatgaag ccagcacttc aaagataggt gaggatcaga tattttactg ccagcaaaga
     2701 ggactcagtg ccgaggatgc tgtttcgttg attgtaaatg gtttttgcaa ggaagtattc
     2761 aaggagttgc cattcgaatt tgcggtggaa gctgaaaggc ttttgagtgt aagtcttgaa
     2821 ggtagtgtgg ggtagaccag aaaattccgg ttgtgattaa ttattaaatt aggagtaaga
     2881 atttatgtta gagataaaag atctgcatgt aaaaattgaa gataaagaaa tattaaaggg
     2941 gcttaacctc actgttaatc ccggagaggt tcattcgatt atgggtccaa acggatcggg
     3001 gaaaagcaca ctttctcagg ttttggcagg aaaggaatca tatgaagtta cttctggctc
     3061 tattacattt aagggaaaag acttacttga gcttgcacca gaagaaagag cctgtgaggg
     3121 agtatttatg gcatttcagt accctgtaga gattgcaggg gtagcaaata catacctgct
     3181 tagggaagcg cttaattcta aaagaaagta tcaggggctt gagcctttga agcatgttga
     3241 gtttataaag aatattaaag aaaaaatgaa aaacctcaaa attgatgaca ccatgctctc
     3301 aaggtctgta aatgaaggtt tttcaggcgg agagaaaaaa agaaacgaga tttttcagat
     3361 gcaggtacta gagcctgatc tgtgtattct ggatgaaaca gattcagggc ttgatattga
     3421 tgcgctgcaa atagtagcag acggcgtaaa tgctatgaga gatgagggaa gatcatttat
     3481 tgtaataact cactatcaga gactcctaaa ctacatagtt cctgattttg ttcatgtact
     3541 tatagacgga aagatcgagc gctctggtga taagagcctt gctcttgagc ttgaagataa
     3601 gggatacacc ggtatagcac agcagccaag cgcagcttag taaaaagaat ggcggagtaa
     3661 attaaagaga tatgcagata atattaaaag acagtacaga tagttactta cagggatata
     3721 agcagcttat tacttctgat accagctcgg caaaatttgc tgagagtaca agggaagaag
     3781 caataaataa ttttgtaaag cttggttttc ctacactaag ggatgaagac tggagattta
     3841 caaatattgt tccaattgtt aaatctcagt attcgataag cacagatgta acaactaaag
     3901 tagaaaatat tgatataaag aaatacttaa tacacggtct agactctcac ctgatggttt
     3961 ttgttgatgg agtttatagt gctgagctta gcaatataaa tgatactgat ggcaaaatta
     4021 ttattaagcc aatttcaaat gctctatctg ataacagaga cattgtagaa aaacatcttt
     4081 ttagctgtgc agacattaaa caagaagcct ttacggcgct caatactgca tattttactg
     4141 acggcgcatt tgtttatgtt ccaggcaaca cagaattgga taaaacagtc gaagtcctat
     4201 atatatcagc agaccctgtt tcatcaatga acagtcccag aaatcttata gtagtagaga
     4261 agagcagtaa agtgcaagtg atagaacatt acgtttctct tacagatagt gtttatttct
     4321 caaacacagt ctctgaagta ataataggag aaaacagcac agttgatcat taccttatag
     4381 aacaggacag caagaaggct tataatatct cttcgttaga ggttaaacag agcgcaaact
     4441 ctaacctgaa ctcacactca tttcttctgg gtggggccat tgtaagaaac aatatacatc
     4501 ctgttcttgg ggggcaggga tgtaattcaa atattaacgg actgttttta ccttcgggca
     4561 gacagcatat agataatttt atgaaagtag aacatgcaag cccgcactgt gacagcaggc
     4621 aggtatataa cggaatatta gctgataaat caaaaggggt atttcacgga agaataatcg
     4681 tgcatgaaga tgcccagaaa acagatgcta aacagacaaa cagaaacctg ttgctctcag
     4741 acactgcaaa aatagataca aaaccacagc ttgagattta cgcagatgat gtgaaatgca
     4801 cacacggcgc tacaattggc cagatggaca aagactcgct tttttatctt cgctcaaggg
     4861 gtattgaaca aaaattagca aaggctataa tgctccgtgc ttttgctagc cagaatattg
     4921 aagatattgg tgataaagtt ataaaaagtt atgttgaaaa acttgttaaa aactggtttt
     4981 ctgaaatagg catggaagca gaattagatt aatcccagtt aataaggtga aaaataaatg
     5041 aatgtagatc aaatggaatt tgtattggat cattacaaga acccgagaaa cagcgggcag
     5101 attgctgatg ctgactactc ttacgaagag ggcaatcctt catgcggtga taaaatcagg
     5161 attgatataa aagtagcaga caacaaaata gcggaggcca ggtttagtgg taaagggtgt
     5221 gcgattagcc aggcatctgc ttctatactg actgagatga ttgagggaat ggagcttgat
     5281 gctataaaag aaatggatgg ccaggaattt ttggaaacac tcggggtgaa tatttctcct
     5341 atacggtata aatgtgcgct tcttgcatta aaggtcctta agaccagcgt gtttggtgac
     5401 ggcgtatttg aatatgaaca ttaaaaaagc gctatgggag aatttataaa agttgcaaaa
     5461 atttcagacg ttcccgcggg tactgctaaa tcgtttcttg tagaaaatga ggttatagct
     5521 gtattcaacc tgaataataa ctactatgcg ctgagagatc agtgctctca tatggacctt
     5581 cccatttctg atggaataat tgagcaatct agagttactt gtgcctatca cggcgcagaa
     5641 tttgatatta aaacaggtaa ggctctgtgc gctcctgctt atgatcctgt tgagtcattt
     5701 gaagtgatgg tgcaggatga gcaaatttat atatcaattg attgattaat atggaaaaag
     5761 tttttaaaaa tatgactaag tttgatcctt acgaaataag aaaggacttc cctatactta
     5821 atcgtaaggt aaacggtaat cctctggtat accttgacaa tgcggccact tctcaaaaac
     5881 cctctcaggt aatagattgt ataaaatcat attacgaaaa taataattcc aacattcaca
     5941 ggggagtaca tacactcagt tatgaatcaa cagtgatgta tgaagatgca cataaaaaag
     6001 ttgctgcatt tataggggca gaggactgga gagaaataat atttactaga aatgctacgg
     6061 aatctctaaa cctggtggct tatagctggg ggcttcacaa tttaaataag ggtgacgagg
     6121 taattatatc aataatggag catcactcca atattgtacc ctggcagatg cttgcaaaag
     6181 ttaaaggaat agtcctaaag tttatagatg tggatgataa gggtaacctg gatttggaaa
     6241 gctttgataa tctgctttca gataaaacaa gagtagtctc ggttattcat gtatctaatg
     6301 tgctgggagt agtaaatccg gttgagtaca tttcaaaaaa agcaaaagat catggggcgc
     6361 tctttatagt agatgctgca caatccgttc ctcacatgaa gctcagtgtg catgaacttg
     6421 attgtgattt tctggctgca tcgggacata agatgcttgg accaacagga atcggctttt
     6481 tgtatggaaa gaaggattta ctggaaaaca tggagccgtt tttttatggt ggtgatatga
     6541 taagcactgt aacaaaaaca tcttcaacat ggaatgagct tccctggaaa tatgaagccg
     6601 gaacaccaaa tatcgcagac ggtatagcgc ttggagctgc aattgaatat cttgaaaaat
     6661 taggactgaa aaatatagaa caatatgagt cagagctttt agactatacg cttgatagac
     6721 taaaagacat accctggata gatatatata ctcctcttac gggtaaaagg gtaggtgtga
     6781 tatcgttcaa tgtaaatggg gtacatcctc atgatgttgc aggtataatg gatgaagacg
     6841 gcattgcagt aagatccggt catcactgca cacaacctct tatgacaaga ctcagcatag
     6901 aaaatgccct aagggcaagc ttttatatct ataacactaa tgaggaagtg gataagttta
     6961 tttcgtccct tcttagagcc tacgatatat ttaaaaagta agattattct cttgtaatat
     7021 taataagttc atactgaggg aaaaactttt tgtgtttatc tttgcagctt gcaagaacct
     7081 gcaccgcatt atctttcttg tccaataaca cattgtaaac atagccattg tgattatttg
     7141 taaatactgt atttatttga ccgctgtcgg ttcttgatac ttgctctaat ctaaaatcac
     7201 taatcttggt atcattattt tccatagcca gaaaatattc ggcagcctgc tcatcggatg
     7261 agtaaaaaca tcttcctctt agaagttcag tgcaaagaga gttttttaaa taacattctt
     7321 ttattttata atagttatct tcatccaccc tgccgtaata aattccttca ggaagcagca
     7381 gtatgttaga ggctaaccta tgaccaccca ggtgagttgt ttgccagact tcaaaatctt
     7441 tttctatact aaaaagatta ttgtacaatt gcttacccat tgtaccacag cacttgtcat
     7501 atgagccgtg agtacataca agaagcagag gtttagctga gatatgatca ctgtttttta
     7561 gcgctttttc aaaatcaata gtcagtatct ggtcatatga atcaaggcta aactcaaaaa
     7621 cttctttctc agatgattta gtaattgcaa tatatagctt taacttggaa cttttactat
     7681 ttcttctaat taactgaatt cgggatttag gatgcattcc tgccagattt cttattacat
     7741 cctttagttc tttagaaatg ctacagttat taaatgcctt ctcttcccac tgcctggtat
     7801 attctatgag gatccaaaaa ttaatgtttg gggctgtgcc aaacattttt tcacctgcac
     7861 tcattgagct accaaaacat atttgggagc tatcaaagca tatttggttc ttattggtca
     7921 ttattgctca ctatttatcg gaatcttaat attttatcaa aattacatag aatactatta
     7981 tttatcaatt tcaaaataag taaaaagtta aggattgatt tattaagcaa taaagtttat
     8041 ccttgtagta tagaattgag aggtagaatg gagggtttta tgagtgtact aagtaaccaa
     8101 agccacaatg aagtaataaa cactttaagt aaacttgtga caagaacaag agattagcag
     8161 gagatatata aaacctctgc agatttctta aatgagatgg aacatgtcga tatggttatg
     8221 atctatgtag tggatgaaga taccaatcag gctgtgctgg aagcacacag gaatctaact
     8281 gctgactata ttgcaagagc ctcaagagta gacagaccta agggtattac ctggagagtt
     8341 atacaaacca atcaatttct aaatgtaaag gatgcccaga ctgatgaatc aattggacct
     8401 gccggaaaag ctttaggtaa acacggcgtt ttgggtatgc ctattacact cacaacaggt
     8461 ggagaggcta agggagtgat atggctttgg agtgatactg aaagggcctt tactgatgaa
     8521 gaggtaaaac ttctaagtgc tattggaaat cagatagcta ctgctattgc ctgggctaag
     8581 caatacgagg atagaagaaa ggtttggcag gaaagattta aggtttaata ttcaaaactt
     8641 ctctttatct cttctaatta attacaattt atgtcaaaaa ttacaatttt tgtagcagac
     8701 gaccatccaa ttgtcaaaga aggccttatt acaattttaa acacacagtc tgattttaaa
     8761 gtagttggag aggctatgga tggaacaaaa gttgtgagtg gagtggaaga aaccaaccct
     8821 gatattcttg tactcgattt agaaatgcct ggcatggatg gtattgaaat aatagagaag
     8881 ctaaacaaat ccggttgcaa agccaaagcc ataatattta cagcctataa aacagatgac
     8941 cgaatatttt cagcatttga actaggagca aaaggttatc tgctaaaagg ctcacctaaa
     9001 gaagaaatat ttgatgctgt tagaaatgta tataacggag gcacctcggt acatcctgat
     9061 gttacggaca gactggtaaa aggagtaaat cagaaactgg acaaattaac acccagggag
     9121 ctcgatgtga tgaaatctct ttccaggggt cttacgaata aagaaatagc gtatgagcta
     9181 tttattagcg aaagaaccgt taagtttcat ataagctcta tattcactaa acttggtgca
     9241 gaaactagga ccgaagccct ttctattgct atacaaaaag gccttctatc tatttagact
     9301 tactactttc aatttgttca aaagcctctt ttattgtatt tagaattaca gtgtcattaa
     9361 aaggcttaat aatatagcca agcggtttgg tctccttggc cctttgtctt gttttcgtat
     9421 ccccataggc tgtaagatat agaaacggta cattgagttt tagtagttcc ctgccggttt
     9481 caataccgtc catatcacca tctacaacaa cgtcaagaat tgcaaggtcg ggtttatatt
     9541 gctcagctat ttgaatagca tcacttccat tttgagctat tcctgctaca gaatacccaa
     9601 ttttttctat ataagatttt atgcttttcg caattatcat ttcatcttct aggattaaga
     9661 tagtcttact tttttccggt ttaattgtac tagtattcat ttataatata tggtatttat
     9721 aatttcaggg gcattgaata aaactctcca tgctattata taagcaaaaa ctgtaccaaa
     9781 acaataatat ggtacagttt atttaatctt tagaataaat tctgataatt tagagtgaaa
     9841 ataaataaca gtaaaattaa tgtattgaat ttatatatta aattgatata aaatcaatta
     9901 ttaagcataa agtctatata actattacaa gatggataaa aaaattctca taatcgatga
     9961 tgagccttta atatctatgc atatagccag gctcttaaca aagtgaagat tttacagtaa
    10021 ttagggcaac gactggaaag gaaggtctaa atgtaattga gacagatact cccgatctga
    10081 taattctgga tctgagactg ccagatgtgc atggtatgga attactggaa caaataagtg
    10141 aaaaaaatct gaaaacaaaa gtagttgttc ttaccggaca cggtaatatt gatactgctg
    10201 tacaggcaat aaagctcggt gcttcagatt atataacaaa gccatttgtt gataataagc
    10261 taaaatcgca gataaaaagc ctcattgaga gtaaaatcga taatatatct gcttctacgg
    10321 ttacaagaaa agttgtaggt gattcacctg ctatgcgtga gctttggaat atgttaagca
    10381 agtttgctcc gcctgatgtt aatatcttgc ttgaaggaga gacaggaaca ggtaaagaac
    10441 ttgttgccca tgccattcat gatatgagca ggtttagtaa cgggccgttt attcctctgg
    10501 attgtgcatc tatgcctgaa actataatag agagcgagat cttcggatat gagaagggag
    10561 cttttacagg tgcagagaat agaaaaccag gcaaatttga aatagcaaag ggcgggacaa
    10621 tatttcttga tgagatatcc aatatgcctc tatccacaca ggctaaacta ctaagggtga
    10681 tacaggagag aaaggtattc ccgcttggat ccaaaaacgt tgaacctatt gatctagact
    10741 gtcgtttagt cgctgcgtcc aacaccaatt tggaggatat ggttaagaaa ggaacattta
    10801 gagaagacct cttttacaga ctaagtacag taaaactcat tattcccccg cttagagaaa
    10861 gaaaagaaga tatcaagaac ctatgtatgc attttgtaga tcttttcaac aggaaactcg
    10921 gtactaaagt tgattcgctg tccgatgatg ttgtaagtgt tttagaaaac tacgattggc
    10981 ccggaaatat cagagaacta gaaaatgtga taaagtatgc ggttattaaa gcagatgaca
    11041 ttattacttt aaatgatatt ccgaaccata tggataaaaa cggtgcagca caaattgctg
    11101 aatctgtaaa cgaacttata agctgtacga ctaataattc cgataataac tcagttgata
    11161 ttaaacttaa aattcaggtt ttagatacga tggacctcaa gcaggtagct aaggatgtgt
    11221 ctgatatcgt aaaaagagct atcatttcca aggtatctaa aacttctaaa ctcagccaga
    11281 ccgagattac taaaaattta aatatagatc ctaaaacata tcgaacaatt attaagaaac
    11341 tgcagtgatt tgattatagt atcgcagtgt tcttgggtgt ttttactaca tttcacaggg
    11401 tagagtaagt aattgagtaa agtgccaggt cttaaatggt atgtaaattg cactataagt
    11461 atataaggca ggttttatta ttaataataa aatgaaacta gaaaacattc ttatagtcga
    11521 agatgaatta attgtagcta aacatataga aaaacagcta attaaatgtg gctatgaagt
    11581 tacggctata gttaataacg gaagtagtgc actaaatgaa gtaaaatcca atccgcccga
    11641 cttaatattg atggatgtaa atattgaagg tgacctggat ggaatacaga catctgcgct
    11701 tattaacaaa caatataaaa ttccaattgt atacttaaca gcgtttaccg aaaaaaatac
    11761 tattgaacga gctaaagaaa ccgaacccta tggatatatc ataaagccat tttcaggcaa
    11821 gaatttagag actacagtag agattgccta tcataagttc cagattgaaa aaaagttaag
    11881 ggacagcgaa agctggctca ttaatacaat gagtaatctg gcagatgcag tgataactac
    11941 agagccaaac ggtcatatta aatatttaaa tcccgtagca gaaagcttta caggatggac
    12001 taatgagcag gcggtgggta aaaatttaga agaaatatta gttctatacg atgaaacgga
    12061 atataaaccc gtaggagatg ttttagagca tatcttgagt gcaaagaatg aaaatgacaa
    12121 ttatgctttt ttaaaatcaa agcagggcaa agatattata atacgtttta aggctgcaca
    12181 tataaagcag gataataagg ataatatagg tgtagtaata gtttttcagg attttactga
    12241 tcatagacta caggagttaa gactattaaa atcacatgaa agacttcaga ttaaagtaga
    12301 aaagaatatt aaagaaataa gttccattag cgctgatctc aaaagtagta aagaaaaata
    12361 taagactttt atagaaaatg tatttgattt gattgtagaa gtagattctg atgggatatt
    12421 tagttatgta aataataatt acaaaaagtt gtttggacac accgagagtg agttgtccgg
    12481 aaagaagata tttgatttta tatatagcga ggacctcgaa gaatttgaga atatctttat
    12541 ggattcaatc caaaataata ccgatttttc acactcatac aggtcaatca acaagagtgg
    12601 tgatcttgtc tggctgtaaa gcacaggcaa taatgttgta gattctgatg gaattacatc
    12661 tatgctcata gcatcaaggg atattacgga aaagaagaaa tatgaa
//
LOCUS       DBNGSV010000239         3054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_250183, whole genome shotgun sequence.
ACCESSION   DBNGSV010000239 DBNGSV010000000
VERSION     DBNGSV010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_250183"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..2481
                     /locus_tag="AC159U_08795"
     CDS             301..2481
                     /locus_tag="AC159U_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HIR3337195.1"
                     /translation="MNKIIFVSNRLPVTIKRDQNNELEAIASGGGLVTALSTFEKMSE
                     KVWIGWPGVLDKRNKSENDEIKELLKQYDYRPVSLNSKQIKEYYEGFCNETIWPLFHY
                     FVQYAIYEKTFWDTYRSVNQKFCDAVLKEARHDSIIWIHDYHLLLLPNMIRQKLPDAR
                     IGFFLHIPFPSSEIFRLIPWCREIIDGLLGSDVIGFHTFDYVRHFAESVRRIVGIEHT
                     LGQFTLGSRAVRVDTFPMGIDYNKFSKSPSEPVVKEKISKLKKEIGGDYKVILSIDRL
                     DYTKGIPNRLQAIEHFLDNNPGYKQKVIFIVVAVPSRTEVEHYKKLKENVEYLTGKIN
                     GKHGSIGWTPIWYLYRSFEFDDLIALYSIADILYITPIRDGMNLVAKEYVATKNDGNG
                     VLILGEMAGTAKELGEAIIINPNDIEKTSNALKKALEMHINDKTARMKSMQKRLKRYD
                     IKKWTNDFMDRLSQIKDVQKQIESKVLAGNGKNSLINDYKKASKRLLLLDYDGTLMPF
                     NENPANVSPDKEVIQILSNLKNDKRNTVVIVSGRDRGTLQSWLGNLTHGMVAEHGVWI
                     KNGSWSTIEKLDNSWKDEIRSIVEMFVDRTPGSLLEEKEFSLVWHYRKVDPSLANIRV
                     GELKYILIHAIANLNLGVLEGNKVVEIKNTGINKGRAVRKWLDSNSWDFILSVGDDWT
                     DEDVFEVLPRDAYSIKIGFGASKASYKIHSYKEVRDLLKKISTL"
     gene            2568..>3054
                     /locus_tag="AC159U_08800"
     CDS             2568..>3054
                     /locus_tag="AC159U_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF-domain containing protein"
                     /protein_id="HIR3337196.1"
                     /translation="MEEQSLRVYKFLSRFPFPNSYLGKIILVAFFGIQVPLITLIIYS
                     VYSNPQGPESSIRILAATVIASVMGTAFTLYVIYYLLAPVSLASQKLREYITDKKMPN
                     LPTYYKDGAGQLMSDVQYTVSHIDKLIRSLEKTSSTDYLTGTYNRFSAETRLKEDIAR
                     SS"
BASE COUNT         1099 a          451 c          598 g          906 t
ORIGIN      
        1 aaaagctcta tatcatggtt tggaaagttt ttaccaatca acggagatat tcggggttta
       61 ttatctttgt taccgagaat agaaatagct agtatagcta taaaacatcc tatgagtaaa
      121 aatgtggata tttgcagtat tttagggata tatttcatat attagtttaa tttcatagac
      181 ttacatatat aatatcttaa atagatgttt attaataaat tattcacttt cccaaaatgc
      241 atctgatata atcttattat atatacatgt tataaaatat cgatttatta aaaatatgca
      301 atgaataaaa taatttttgt atccaacagg cttcctgtaa caataaaaag agatcaaaac
      361 aatgaactcg aagccatcgc aagcggcggc ggtcttgtca cagcacttag tacatttgaa
      421 aaaatgtctg aaaaagtgtg gattggctgg cccggtgttt tagataaacg caataaatca
      481 gagaatgatg agataaaaga actattaaaa caatatgatt acaggcctgt atcacttaac
      541 agtaaacaaa tcaaagaata ctatgaaggt ttttgcaatg agacgatatg gccgctattt
      601 cactattttg tccaatatgc aatttatgag aaaacatttt gggatacata cagaagcgta
      661 aatcaaaaat tttgtgatgc agtattaaaa gaagcccggc acgattccat aatatggatt
      721 catgactacc acctgctgct tctacccaat atgataagac agaaacttcc tgatgcaaga
      781 attggatttt tccttcatat accgtttcct tcttctgaga tcttcaggct tattccatgg
      841 tgcagggaga ttatcgatgg tctgcttgga tcagatgtta taggatttca cacgtttgat
      901 tatgttagac actttgccga aagtgttaga agaatagtag ggatagaaca cacacttgga
      961 cagttcactc ttgggagcag ggcagtgcgg gttgatactt ttcctatggg tatagactat
     1021 aataaatttt cgaaatcccc atcagaaccg gtagtcaaag aaaagatatc taagttaaaa
     1081 aaggaaattg gcggagatta taaagtaata ctatcaattg atagactgga ttatactaag
     1141 ggtataccaa acaggttgca ggcaatagag catttcctgg ataataatcc cggatataag
     1201 caaaaagtaa tatttattgt tgtagccgtt ccgtcaagaa cagaggtgga gcattataaa
     1261 aaattaaaag aaaatgttga atatcttacc ggtaaaatta atggaaaaca tggttccata
     1321 ggatggacac ctatttggta tctatatagg tcatttgaat ttgacgatct catagctctc
     1381 tattctattg cggatatttt gtacattaca ccaataagag atggcatgaa ccttgttgca
     1441 aaggaatatg ttgcaacgaa aaatgatggt aatggggttt tgatcctggg agaaatggca
     1501 ggtactgcta aggaattggg agaagccatt attattaacc ctaatgatat tgagaaaact
     1561 tcaaatgcgc tcaaaaaagc tttggaaatg catataaatg ataaaactgc aagaatgaaa
     1621 tcaatgcaga aaagacttaa aagatatgat attaagaaat ggacaaatga ctttatggat
     1681 agactaagtc agattaaaga tgtacagaaa caaattgaat ccaaggtgct agcgggcaat
     1741 ggtaagaata gtcttataaa tgattataaa aaggcatcaa agaggctgtt gcttttagac
     1801 tatgatggca cactcatgcc gtttaatgag aatccggcaa atgtaagccc ggataaagaa
     1861 gtgatacaaa tactatcaaa tctcaagaat gataaaagaa acactgttgt aattgtaagc
     1921 ggaagggaca gaggcacatt gcaatcctgg cttggtaatc tcactcacgg aatggtagca
     1981 gagcatgggg tttggataaa aaatggaagc tggagtacta ttgaaaaact cgataatagc
     2041 tggaaggatg aaataagatc tattgtagag atgtttgtgg acaggactcc gggatcactt
     2101 cttgaagaaa aagaattttc actcgtatgg cactacagaa aagttgatcc atcactagca
     2161 aacattaggg taggggagct gaaatatatt ctaattcatg ccatagctaa tctgaatttg
     2221 ggggtacttg agggcaacaa ggttgttgaa ataaagaata ccggcatcaa taagggaagg
     2281 gctgtgagaa aatggttgga ttcaaatagt tgggatttta ttttatctgt tggagatgac
     2341 tggacagatg aagatgtatt tgaagtacta cccagggatg cttattctat aaaaataggg
     2401 tttggggcat caaaagcctc atataaaatc cattcttata aagaagtgag agatctactt
     2461 aaaaaaataa gtacgcttta gtttataata atactaaata cctatatcta tatagaaact
     2521 ttctgaaatt agagtaatat ataaaattat ttaattgagt aataattttg gaagaacaaa
     2581 gccttagagt atataaattt ttatcacgtt tcccttttcc caatagctat ttagggaaaa
     2641 tcatacttgt tgcgtttttt ggtatacagg taccccttat tacattaatt atttattccg
     2701 tctattctaa tccccagggg ccggaatcgt ctataagaat acttgcagct acagttatag
     2761 cctctgtaat gggaacagct tttacacttt atgtaatata ttatttatta gcacctgtat
     2821 ccctggcttc acaaaaatta agagaataca taactgataa aaaaatgcct aatcttccta
     2881 catattataa agacggagca gggcagctga tgtcagatgt gcagtacacg gtttctcata
     2941 tagataaact cataagatca ctggaaaaaa catcgagcac tgattatctt actggaactt
     3001 ataacaggtt ttcagcagag acaagattaa aggaagatat tgcaagatca tcca
//
LOCUS       DBNGSV010000240        11975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_527801, whole genome shotgun sequence.
ACCESSION   DBNGSV010000240 DBNGSV010000000
VERSION     DBNGSV010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_527801"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="AC159U_08805"
     CDS             <1..451
                     /locus_tag="AC159U_08805"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR3337197.1"
                     /translation="FVIGAIEYAKELKASSVLISFNPTKKRSADIVISPILGPEVIVG
                     STRMKAGTATKMILNMITTAVMIKLGKTYKNLMVDVQPKSLKLRSRAVRIVKQLVGTT
                     ENKSKSLLESANWQVKPAVVMGIKKVTYNRAKEILTQNDGFLRKALK"
     gene            470..1642
                     /locus_tag="AC159U_08810"
     CDS             470..1642
                     /locus_tag="AC159U_08810"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR3337198.1"
                     /translation="MNPILNLSNKKKKIVIGLMSGTSMDGIDAAVVEIKESGINSKIK
                     PLGFVCKKYSARVKERLLSVNAKTRIDEISELNVLISKEFVRTALQCATKFVSDSSKV
                     DLIGSHGQTIYHNPPSRKIGIPSTVQLGDIDVISHNTKVTTVGDFRSKDIAAGGEGAP
                     IMPYVDYILFNKQSPVLVQNIGGIANVTVVTNKMEEMIAFDTGPGNMLMDNMVRLYSE
                     GKKLFDKNGAIARAGNINHKLLESLLLEEYYRLKPPKSTGAEKFGSIMVKKLYKQVIS
                     NKIRYEDMLRTLLQLTVETLALAYEKFIIPKYHPQKVILSGGGAKNLFMLSELKKRLG
                     DMDILTSDDFGIPSDAKEAIGMAVLANEIISGNKTNIPSCTGAKVKVPLGKISYSF"
     gene            1652..3400
                     /locus_tag="AC159U_08815"
     CDS             1652..3400
                     /locus_tag="AC159U_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3337199.1"
                     /translation="MDNIINSFAAVDWVIILSYLVVTIGIGLYFTKRASSNITDYFVS
                     GRNLPWWLLGTSMVATTFAADTPLVITGWVRTEGIWKNWFWWNYIFGHVLAVFLFSRL
                     WRRAEVLTDNEFIELRYSGKPAAFLRGFKALYFSTLFNFIVMGWVISAMAKVFSVFFG
                     VDTYAAVVVCLVIAVTYTALSGIWGVAITDFLQYIIAIIGSVILVHFVISSPDIGGYS
                     GFLSRLGEVEGNLKFFMTPETGTPVSDGFLSSSFFSFLVFISVIWWSSHNADGGGYII
                     QRMSSAKNENHAVAGTAWFVINHYIIRLWPWVLVAVASLLVFPYAEHKDADQEIYYLL
                     MIKKFLGPGLQGLLFVTFLAAFMSTITTHLNWGSSYLVNDIYKRFIDKNGSDRRYVLV
                     SRIFTIVITILAGIFALYIKNIGKAWIFLWAMSAGIGLVLILRWFWWRINAWSEISAL
                     ASSIITIIAMILYTNLKGISLELKHQVIVIPISITTWVITTYLTAPEPTQRLQSFYNK
                     IRPWGFWGPVSTNNENKEPLTDLLLNWVLGICVVISGMLGVGKLLLGQFGLGFILFII
                     SVFCSFILYYRIFKIK"
     gene            3467..4057
                     /locus_tag="AC159U_08820"
     CDS             3467..4057
                     /locus_tag="AC159U_08820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR3337200.1"
                     /translation="MAIKIKVLKAPELTFFEKLYFPQILKGMFITLKHLLGSKSITVQ
                     YPEEVKELPPRYRGLHIMPGDDDQKIRCVACKLCEVACPTQAISIVAEPADDYGVERR
                     PKIYNIDFMRCVFCGFCVEACPCDALRMGMKYELASYNRAGLVHTKEVFFEPRVRSEA
                     PRDNANFLYEMGKGKILDSPEAAEKVKELSGIYTPK"
     gene            4176..4739
                     /locus_tag="AC159U_08825"
     CDS             4176..4739
                     /locus_tag="AC159U_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337201.1"
                     /translation="MHKLILLLLGCLILILNGINVVYSQDIEPAARQVDTSKEAARGY
                     NNRGLLYLDKRLYDNAIKDFDKAIEYHPRYAEAYNNRGLAYNKKAMYQDALNDLISAI
                     KIDKAFAGAYANRCFTYINMGELQKAELDCSMAISLDKQLYKAYLYRGIIYQANDDKG
                     KAKEDFNTVINNSSDNLLVIQAKALLK"
     gene            4764..6128
                     /locus_tag="AC159U_08830"
     CDS             4764..6128
                     /locus_tag="AC159U_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3337202.1"
                     /translation="MKTLLFPPGPKGLPLLGSSYHYFTDILGFLTHMRKTYGEIAFFR
                     LGKRNIYMVSDPEIVKDVLVTNNKNFSKSRALKRAKMVVGEGLLTSEAEVHLKHRRMI
                     QPVFLQRRISKYASVMSEHSHRISEKWHDGQILDMHKEMMKLTFSIVVKTLFNSDIEK
                     DPDEIGAALTDVMKQFTRLVFPFSEYLDKLPLPGVKKCNDALASLDRTIYEFISERRG
                     NEDKYDDLLSLLLSVRSEDSESEGFSDQQIRDEAITLFIAGQETTANSLSWSLYLISR
                     HPGIDVRLHEEISSVLSGRLPSMDDYNKLQYTRMVFTEAMRLYPPAWTVVRRALSDYH
                     LKDYIVPQGDDIFMSQYVIQRDPKYYPEPLEFRPERWENDYMKTIPKFAYYPFGGGPR
                     LCIGEGFAWMEGVLVLATILQNWKMNLVDNKPVKPDPLITLRPKGGLKMRLEKRVSQS
                     LNNF"
     gene            complement(6125..6433)
                     /locus_tag="AC159U_08835"
     CDS             complement(6125..6433)
                     /locus_tag="AC159U_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337203.1"
                     /translation="MSESLKCNLDNLNEQEKEKRDALFDKMKFAITDVIETENGYDIS
                     VFSNVVNIIDVDNITQLEKKCCPFLEFDVISEGGYTVIKVSGPPSAKEVIVEQFGLMV
                     "
     gene            complement(6477..6956)
                     /locus_tag="AC159U_08840"
     CDS             complement(6477..6956)
                     /locus_tag="AC159U_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337204.1"
                     /translation="MKILSIITVLIIAVAAFFLWPASDDPTEIEKVLKKMIAAGEAGN
                     FEGVTEHVSFDYRDEYGATFFVVKNIVQKVFEKFDKFETKYDGLSVTMNESDDGSKIA
                     IANLNMNIKGKKSNMFFSLLGQDESYENITITFKKTKLGEWKVIKSEGLDRAIEEGY"
     gene            complement(6985..9333)
                     /locus_tag="AC159U_08845"
     CDS             complement(6985..9333)
                     /locus_tag="AC159U_08845"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3337205.1"
                     /translation="MQLHFSNKLIKSFLLRNPVYSPLFFFVTGIISTEYIELDFKFII
                     ILSFLLVLLTYYKLNFKYLLFIPLGILFSTHSPPDEKDLELLVNNKVDIEGILIKPVE
                     RRDQSSRLFLHVNMATVDGKRHNLDVKVMITLNEHLYGIYKGHRIRILGIKLKQFRNY
                     KNPGNFDVENYYKRKQIYLSGFIDDKSDIISFGRASSKISFFELVEKSREGFVRFIRS
                     NLGFPNSEIYSALTIGEKKGIPHEIRNSFSSLGISHLLAISGLHLGAIVLIFYFIFDW
                     LLKRSERLLLNYNVPRITAALTILPIICYMFVSGFATPVIRATIMAVVYLLSIIIGRA
                     HYKLNTLLASAFIILLIDPSAIYDLSFKLSFLSVLGILTIHHYYPLNIYTFKDKLLTS
                     LKTTFIATIFTLPIIINSFGIVSLLSVPANLIFIPLVEFIIVPLGLISYILFHISSSA
                     TVYLIEANNHLINLLLSLVQSLSSLNIASVTVPYLNNLSVTLYYLSVFLIFAFISTGS
                     KWLKLLTPAVIFLFLFSASFGFFKDITNKNFEAYFLDSGTRNTVFIRTQSGKHILING
                     GISRFNQNGFTEKSVITPFLLKTGISKIDHIILTQTNEDILEGTSHLISKFNTDYLWT
                     NGARLNSKVWEEIKSANLKWINLQTGYEPLHLGKTNITMQFSPNNGTQLSGDNSVSIN
                     IKNENLRLLITDASVYKQSIKIFNDKNPDDQIKKIIYLPYMNNDYAAIDYIQTYKPDV
                     LITKKDKIVNESYKFKKYQTSKDGMIKVVLKNKSLKILPHNR"
     gene            complement(9302..9781)
                     /gene="ispF"
                     /locus_tag="AC159U_08850"
     CDS             complement(9302..9781)
                     /gene="ispF"
                     /locus_tag="AC159U_08850"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR3337206.1"
                     /translation="MYRIGNGFDAHKFAKDRILIIGGVEIPYKYGLSGHSDADVLCHS
                     ICDAILGAANCGDLGTHFPSTEPKFKNISSLVLLKQSYDIVKSKGFVIENIDTTIICE
                     KPRLSSFISKMSDRISESLGVADSLISIKATTTDGLGFTGRAEGIAAYATALLKQVD"
     gene            complement(9774..10478)
                     /gene="ispD"
                     /locus_tag="AC159U_08855"
     CDS             complement(9774..10478)
                     /gene="ispD"
                     /locus_tag="AC159U_08855"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR3337207.1"
                     /translation="MASNDYISAIIVAGGTGRRFGNGQKKQFIQLAGKPILFYSIDAF
                     QRCELVSEIVLVLPADNIEFYKNNLSGIGKFDKVRTITEGGQTRQQSVLNGLQKLSEI
                     TDIVLVHDAARPLVNISTINRVIEQTIVSDCAICAVSVSDTVKQASENLIEKTVPRDN
                     LWLAQTPQGIKYELIKRAYKNALDNNLSCTDEASLVEELGVKPAIVPGPKNNIKITTP
                     DDLKLAEFYLMEHADV"
     gene            complement(10471..11487)
                     /locus_tag="AC159U_08860"
     CDS             complement(10471..11487)
                     /locus_tag="AC159U_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR3337208.1"
                     /translation="MLIKLLVLALVTFLGFFFSYNPENVDFSIKITILFGALAAVFIF
                     IIEPSTEEISPKSAAGSAGGIVAASILYVLIKELMNIAPIPAEYAPFADVAALLVLVY
                     TGITIGIKKGADSDNRVGGKYSSKKSSFRSMILDTSVIIDGRIADVAEAGFINGEVLV
                     PKFIIKELQYIADSGEPIKRVRGKRGLDILKRMQQDITTVNIKITGHDFPKIREADLK
                     LVELAKKINGVIVTNDSNLNKVAGLQGVKVLNLNELSNALKPIVLPGETINILIAKEG
                     KEEKQGIGYLEDGTMVVVDDARNKIGKQMQVSVTSVLQTPTGRMVFSKIKGTEHDMVS
                     EING"
     gene            complement(11596..>11975)
                     /locus_tag="AC159U_08865"
     CDS             complement(11596..>11975)
                     /locus_tag="AC159U_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337209.1"
                     /translation="SNTIIPDMVAKSLESQFKNIEITRTSSEFFRPRSNISGKNILLV
                     KGEVTNYNRGCKYCEWFLRRNDKGKSSVTVRINLIDKHTGEIIADKSIKGRAKSPGYG
                     ESRYIRVRDEIVSLVLKITAKNS"
BASE COUNT         3712 a         2136 c         2248 g         3879 t
ORIGIN      
        1 ttttgtaata ggagcaattg aatatgccaa agaacttaag gcttccagtg tacttatatc
       61 tttcaatcct acaaaaaaga gatcggctga tattgtaata tcaccaatac tcgggcctga
      121 ggttattgtt ggctctacaa gaatgaaagc aggtacagct accaagatga ttctcaatat
      181 gataacaaca gcagtgatga taaagcttgg aaagacatat aaaaacctta tggtagatgt
      241 acaacctaaa tcactcaagc ttagaagcag agctgtaaga atagtaaaac agcttgtagg
      301 aactacagag aataaatcaa aaagcctgct tgagtctgca aactggcagg tgaaacctgc
      361 agtagttatg ggaataaaga aagttacata taaccgtgca aaagagatac ttacacaaaa
      421 cgatggtttt ttaagaaagg cactaaagta gtttttttat aaaatattaa tgaatcctat
      481 cctgaattta tctaacaaga aaaagaagat tgtaatcggc cttatgtctg gaacttctat
      541 ggatgggatc gatgctgcgg tagttgaaat taaagaaagc ggtattaact ctaagatcaa
      601 accccttggt tttgtttgca agaaatacag tgccagagta aaagaaagat tattaagtgt
      661 aaatgcaaaa acacggatag atgaaatttc agaacttaat gttttaatat ctaaagagtt
      721 tgtaagaact gcacttcagt gtgctactaa atttgtctcc gattcgagca aggtcgatct
      781 aataggttcc catgggcaga ccatctatca taatcctcct tcgagaaaaa tcggtatacc
      841 atcaactgtc cagcttgggg atatagacgt catctctcat aacacaaaag tcaccacggt
      901 tggagatttc agatccaaag atatagccgc tggaggtgag ggggctccta tcatgcctta
      961 tgtagactac atattgttta ataaacaaag ccctgttctt gtacaaaaca taggtggaat
     1021 tgccaatgta acggtagtta ccaataaaat ggaggaaatg atagcttttg atacaggccc
     1081 gggaaatatg cttatggata atatggttag gctgtattca gaaggtaaaa aactattcga
     1141 taaaaacgga gcaattgccc gtgccggaaa tattaatcat aaactgctcg aatcattact
     1201 tttagaggag tactacagac taaaacctcc aaagtctacc ggagcagaaa aatttggttc
     1261 tattatggta aagaaattat ataaacaagt tattagtaat aaaattagat atgaagatat
     1321 gctaagaact ctgctccagc ttactgtaga gacattagcg ctggcttatg aaaaatttat
     1381 tattccaaaa tatcatcccc aaaaagtcat tttaagcggt ggcggtgcca agaacttatt
     1441 catgttaagt gagcttaaaa aaaggcttgg tgatatggat atattaacat ccgacgattt
     1501 tggtattccc tcggatgcca aagaagcaat agggatggca gttctggcaa atgagattat
     1561 atcgggtaac aagaccaata ttccttcatg cacaggggca aaggtaaaag tacctcttgg
     1621 aaaaatatca tacagttttt aacatcaata aatggataat attataaatt catttgcagc
     1681 tgtagattgg gtaattattc tctcatacct tgtagtcaca attggaattg gcctgtattt
     1741 taccaaaaga gcaagctcaa acataacaga ctactttgtt tcaggtagga acctgccatg
     1801 gtggcttctt ggcacatcga tggttgctac cacatttgca gcagacaccc cgcttgtaat
     1861 cacaggctgg gtgcgcaccg aggggatttg gaaaaactgg ttttggtgga actacatttt
     1921 tggtcatgtg cttgctgtat ttcttttttc aaggctttgg agaagggcag aggtactcac
     1981 tgataatgaa tttatcgagc ttcgctacag cggaaaaccg gcagcatttt taagaggatt
     2041 caaggctctt tatttttcca cactgtttaa ttttattgta atgggctggg ttataagtgc
     2101 catggccaaa gtgttttccg tcttttttgg agtggatact tatgcagccg ttgttgtctg
     2161 ccttgtaatt gcagtaacat acacggcact ttctggaatt tggggagtgg ctataacaga
     2221 ttttttacag tatataattg ctattattgg ctcggtaatt ctagtccact ttgtaattag
     2281 ctcacctgat ataggcgggt actccgggtt tctttcaagg cttggcgaag tggagggaaa
     2341 ccttaaattt tttatgaccc ctgagacagg cactcctgtt tcagatggat ttttatcctc
     2401 aagcttcttt tcctttctgg tatttataag tgttatatgg tggtcatcgc ataatgcaga
     2461 tggaggcggt tacataatcc aaagaatgtc ctcagcaaag aatgaaaacc atgccgttgc
     2521 cggcactgcg tggtttgtta taaaccatta tataataagg ctctggccgt gggtgcttgt
     2581 ggctgtagca tcgcttcttg tatttccata tgcagagcac aaggatgctg atcaggagat
     2641 ttattacctt ttgatgatta agaagttttt aggccccggg ctgcaggggc ttctgtttgt
     2701 cacatttttg gctgctttta tgtctacaat cacaactcac cttaactggg ggtcgtctta
     2761 tcttgtaaat gacatataca agagatttat tgataaaaac ggctcagaca gacgttatgt
     2821 gcttgtatcg aggatattta caatagtaat aaccatactt gccggaatat ttgccctgta
     2881 tattaaaaat attggaaagg catggatttt cctttgggca atgagtgccg gaatagggct
     2941 tgttctcatt ctcaggtggt tttggtggag aattaatgca tggtccgaaa tttctgcgct
     3001 tgcaagctcc attatcacaa ttatagcaat gatattgtat accaatttaa aaggaatatc
     3061 cctcgaacta aagcatcagg taattgtaat acctatttca atcaccacct gggttattac
     3121 tacttatctt actgcccctg agccgactca aagactacaa tccttttata acaaaattag
     3181 accctggggt ttttgggggc ctgtaagtac taacaatgaa aacaaagaac cgttaaccga
     3241 tcttctgctt aactgggtat taggcatatg tgtagtaata agcggtatgt taggagtggg
     3301 taaactgtta ttaggtcagt ttggtttagg atttatatta tttataattt cagtcttctg
     3361 ttcctttatt ctttattata gaatttttaa aataaagtag agattgtttt caatatcctt
     3421 attttgtgtt aataattatc acagtatttt ttttagataa aacaaaatgg ctataaaaat
     3481 taaagtacta aaagcccctg agcttacgtt ttttgaaaaa ctttattttc ctcagatatt
     3541 aaaagggatg tttatcaccc ttaagcatct gcttgggtct aaatcgatta cggtacagta
     3601 tcctgaagag gtaaaagagc ttcctcccag gtacagaggg cttcatatca tgcctgggga
     3661 tgatgatcaa aaaataagat gcgtagcgtg taagctttgt gaggtagcat gccccactca
     3721 ggcaatatca attgtagccg agcctgctga tgattatggt gttgaaagaa gaccaaagat
     3781 atacaatatc gattttatga ggtgtgtgtt ttgcggtttt tgtgttgagg cctgcccttg
     3841 tgatgcacta aggatgggta tgaagtatga gctagcttca tacaatagag ctgggctagt
     3901 acacacaaaa gaggttttct ttgagcctag agtcagaagt gaagcaccaa gagacaatgc
     3961 aaatttctta tacgaaatgg gcaaggggaa aatcctcgat tctccagagg ccgcagaaaa
     4021 ggtaaaagag ttatccggaa tttatacccc taaataagta tttcattata tatatttagt
     4081 acaccactta ctactgattc atcaacactc ccagttttca ctcttgaaat ttagattgct
     4141 aaaatagtat aatgatgctg aagagattaa tcattatgca taaactaatt ttactacttc
     4201 taggatgtct aattttaata ttaaacggca taaatgttgt ttattctcag gatatagaac
     4261 ctgctgcgag gcaagttgac acgagcaaag aagctgcaag gggatataac aacagggggc
     4321 tattatattt agacaagaga ctctatgata atgcaattaa agattttgat aaagccatag
     4381 agtatcaccc gcgctatgca gaggcctaca ataacagagg cctggcttat aacaaaaaag
     4441 ccatgtatca ggatgcacta aatgatctga tttcagctat taaaatagat aaggcatttg
     4501 ccggtgctta tgctaaccgc tgctttactt atataaatat gggtgaatta caaaaagcag
     4561 agcttgattg cagcatggct ataagtcttg ataagcagct ttataaagcc tatttatata
     4621 gagggataat ctatcaggct aatgacgata aaggaaaagc taaagaggat tttaatacgg
     4681 ttataaacaa tagctcagat aatctgctgg ttatacaggc taaggctctt ttgaaataat
     4741 cttttaaatt aacccaaaac aatatgaaaa cacttctttt cccaccaggt cctaagggat
     4801 tacctctgct aggctcatca tatcattact ttacagacat actgggattt ttaacacaca
     4861 tgcgtaagac ttacggtgag attgcctttt tcagacttgg aaaacgaaat atatatatgg
     4921 taagtgaccc ggagattgta aaggatgtgc ttgttactaa caataaaaac tttagtaaga
     4981 gcagggcgct taaaagggca aagatggtcg tgggtgaggg acttttaaca agtgaggcag
     5041 aagttcatct aaaacacagg cgtatgattc agcccgtgtt ccttcaaaga agaatatcca
     5101 aatatgcatc tgttatgagc gagcactctc atagaatatc agaaaaatgg cacgacgggc
     5161 agatattgga tatgcacaag gagatgatga agcttacatt tagcattgta gtaaaaacgc
     5221 tttttaattc tgatattgaa aaagaccctg atgaaattgg agcagcgctt acagatgtaa
     5281 tgaagcagtt tacaaggctt gtttttccgt tttcagagta cctagataaa ctgccacttc
     5341 ccggagtaaa aaaatgcaat gatgctcttg caagtctcga taggactata tatgagttca
     5401 tttcggaaag gcgaggcaat gaagacaaat atgatgacct gctttcactg cttctctctg
     5461 taagaagtga agacagtgag agcgaaggat tttcagatca acaaataagg gatgaagcga
     5521 ttactctttt cattgcaggg caggagacta cggcaaattc actatcatgg tctctgtatt
     5581 taatatctcg gcaccctggg attgatgtta gattacatga agagattagc tctgttctca
     5641 gtggcagact tcccagcatg gacgattata acaaactgca atacacaagg atggttttta
     5701 ctgaagccat gaggctttac cccccggcat ggactgtagt taggagagca ctatcggatt
     5761 atcatttaaa agattatatt gtaccgcagg gtgatgatat atttatgagc cagtatgtga
     5821 tacagcgcga tcctaaatat tatccagagc cattagaatt caggccggag cgctgggaaa
     5881 atgattatat gaaaacaata cccaaatttg cgtactatcc ttttggtggc ggccctaggc
     5941 tttgtattgg agagggattt gcctggatgg aaggagtact cgtgctggct acaatactac
     6001 agaactggaa gatgaatctt gttgataaca aaccggttaa gcctgatccg cttatcacac
     6061 taaggccaaa gggcgggctt aaaatgcgac ttgaaaaaag agtaagccaa agtcttaaca
     6121 acttctaaac catgagtcca aactgctcta caattacctc tttagcactt ggggggcccg
     6181 atactttgat tactgtgtat ccaccttcgg aaataacatc gaactccaaa aatgggcagc
     6241 actttttttc cagctgagtg atgttatcaa catctattat gtttactaca tttgaaaaga
     6301 cgctgatatc ataaccgttt tcagtttcaa taacgtctgt tattgcaaac ttcattttat
     6361 caaaaagcgc atctctcttt tccttttcct gttcgtttag attgtccaga ttgcatttta
     6421 gtgattcgct cataactaaa atattaaact aaatggatta ttttaacaat atatgcttaa
     6481 tacccctctt caattgctct gtccagacct tcggatttta ttactttcca ttctcccagc
     6541 ttggttttct tgaatgtaat tgtgatgttt tcataagatt cgtcttgtcc aagcaaacta
     6601 aagaacatat tggatttttt cccttttatg ttcatgttca aattagcaat agctattttg
     6661 ctgccgtcat cagactcatt catggttaca gagaggccat cgtatttagt ctcaaactta
     6721 tcaaattttt caaagacttt ttgaactatg tttttaacaa caaaaaacgt agcaccatac
     6781 tcgtctctat agtcaaaaga gacgtgttct gtgactcctt caaaattgcc agcctcgcca
     6841 gctgcaatca tttttttcaa aaccttttca atttctgtag gatcatcaga tgcaggccac
     6901 aagaaaaatg cggctacggc gattatcaaa accgttataa tagatagaat tttcatatta
     6961 ttaataataa agtatttatt ggatttatct attgtggggg agaattttaa gtgatttatt
     7021 tttcaaaaca actttgatca ttccgtcctt gcttgtctga tatttcttaa atttataaga
     7081 ctcgttaaca attttatcct tcttagtaat taaaacatcg ggtttgtatg tttgtatata
     7141 atcaatagct gcatagtcat tattcatata tggtaagtat ataatcttct ttatttgatc
     7201 atctggattc ttatcattaa atattttaat actttgttta tagacactag catccgtaat
     7261 caataatctt aaattttcat ttttaatatt tatggaaacg ctgttatccc cggacagctg
     7321 agtaccattg tttggactaa actgcattgt gatattggtt ttgccaaggt gaagtggttc
     7381 atatcctgtt tgtaaattta tccattttaa attggcgctt tttatttcct cccatacttt
     7441 ggagtttaat ctcgctccat ttgtccataa ataatcggta ttgaatttac ttattaaatg
     7501 gcttgtacct tctaaaatat cctcatttgt ttgagttaaa ataatatgat caattttgga
     7561 tataccggtt tttaataaaa acggagtgat aacacttttt tcagtaaaac cattttgatt
     7621 aaatctggaa atccctccat ttataaggat atgttttcca ctctgtgttc ttataaatac
     7681 agtgtttctt gttcccgagt ctaaaaaata agcctcgaaa tttttatttg ttatgtcctt
     7741 aaagaatcca aaagaagccg aaaatagaaa taaaaaaata acagccgggg ttagtaattt
     7801 gagccatttg gatccggtag atatgaatgc aaaaatcaaa aatacagaca aatagtaaag
     7861 agtcacactt aaattgttta aatatggcac ggtaacagac gctatattca agctggacag
     7921 gctctgcaca agagagagta ataaatttat tagatgattg tttgcttcta ttaaataaac
     7981 tgtggcagag ctgctaatat gaaacagtat atagcttata agccccaagg ggactataat
     8041 aaattcaaca agtggtataa agatgagatt tgcaggaact gagagcaggg atactatacc
     8101 aaacgagtta attattatgg gaagcgtaaa gatggtggct ataaacgtag ttttaaggct
     8161 tgtaagaagc ttatctttaa atgtatatat atttagagga tagtagtggt gtattgtaag
     8221 gattcccaaa actgagagaa aggatagctt gaacgataga tcataaatag ctgatggatc
     8281 aatcagaaga attataaaag ctgaagctaa tagcgtgttt agtttatagt gagctcttcc
     8341 aataattata gatagtaaat agactacggc cattatggtt gctcttataa caggagtcgc
     8401 aaatcctgat acaaacatgt agcaaatgat gggtaaaata gtaagtgctg cagttattct
     8461 cggcacatta taattaagca gcaatctctc tgatctttta agcagccagt caaaaataaa
     8521 atagaaaata aggacaattg ctcccagatg aagacctgat atggctaaca ggtgagatat
     8581 tccaagagaa gaaaaactgt ttctaatctc atgtgggatg cccttttttt cgcctatcgt
     8641 gagtgcggaa taaatttcac tatttggaaa accaaggttt gatcttataa accgtacaaa
     8701 accctctctg cttttttcta ctaattcaaa aaatgaaatt tttgatgaag cccttccaaa
     8761 agaaatgata tctgatttgt catctataaa accacttagg taaatctgtt ttctcttata
     8821 ataattttca acatcaaaat tgccagggtt tttgtagttt ctgaactgct taagtttaat
     8881 acctaatatt cgtatcctgt gccctttgta aatgccgtat agatgttcgt ttagagttat
     8941 catgacctta acatcgaggt tatgtctttt gccatcaacc gtggccatat ttacatgtaa
     9001 aaaaagtctt gagctttggt ctcttctttc tactggttta atcaagatgc cttcaatatc
     9061 tactttattg tttactaaaa gctctaaatc tttttcatcc ggtgggctat gtgtagaaaa
     9121 tagtattccg agcggaataa acaggaggta tttaaagttt aatttatagt aggtaagtaa
     9181 aacaagtaag aagcttaaaa taataatgaa tttaaaatca agctctatat attcagtgct
     9241 tataatccct gtaacaaaaa aaaatagagg tgaatatacg gggtttctta aaagaaaaga
     9301 tttaatcaac ttgtttgaga agtgcagttg cataggccgc tattccttct gccctgcccg
     9361 taaacccaag cccgtcggtt gttgttgcct ttatacttat taatgaatca gcaactccga
     9421 gtgactcaga tattctgtct gacatttttg agataaagct gctaagcctt ggcttttcgc
     9481 atattatagt tgtatcaata ttctcaataa caaagccttt ggactttacg atgtcatagg
     9541 actgttttaa taaaacaagg cttgatatgt ttttgaattt aggctcggtg cttggaaaat
     9601 gtgttccaag atcaccgcaa tttgcagctc caagaatcgc atcacagatg ctgtgacaga
     9661 gtacatctgc atccgaatgc ccgctaagcc catatttata tggaatttct accccaccta
     9721 taataagtat tcggtctttt gcaaatttgt gcgcatcaaa tccatttcct attctataca
     9781 tcagcatgtt ccattaaata aaattctgct agtttcagat catcaggtgt ggttatttta
     9841 atattattct taggccctgg tactattgcc ggctttacgc ctaactcttc aaccaggctt
     9901 gcctcatctg tacagctaag gttgttatca agtgcgtttt tgtatgcgcg cttaatcaat
     9961 tcatatttta taccctgtgg agtctgagca agccacagat tatctcttgg tacagttttt
    10021 tcaatgagat tttctgatgc ctgcttcacg gtatcgctta cggatacagc acatattgca
    10081 cagtctgaaa ctattgtttg ctctataact ctgtttatag tacttatatt tacaagcggc
    10141 cttgcggcgt catgcactaa aacaatatct gttatctctg ataatttttg aagtccattt
    10201 aatacggatt gctgtcttgt ttgcccgcct tcagtaattg ttcttacttt atcaaattta
    10261 ccaattcctg ataagttatt tttataaaat tcaatattat cagcgggtag aactaacaca
    10321 atttctgaga ccagttcgca tctttgaaag gcatctattg agtaaaataa tattggcttt
    10381 ccggcaagtt gtataaactg ctttttttgt ccatttccaa agcgccttcc agtaccgcct
    10441 gctactataa ttgccgagat ataatcattg ctagccattt atttcagaaa ccatatcgtg
    10501 ttctgttcct tttattttag aaaaaaccat ccttccggtt ggagtctgaa gtacgcttgt
    10561 aacagaaacc tgcatttgtt ttcctatctt gtttctagcg tcgtccacta caaccattgt
    10621 gccgtcttcc agatagccaa taccctgttt ttcttctttc ccttctttag caatgagaat
    10681 attgatcgtc tctccaggaa gtactatcgg ttttagggca tttgagagtt catttaagtt
    10741 taacaccttc actccctgca ggcctgcaac cttatttaga ttagaatcgt ttgttacaat
    10801 tactccattt attttctttg caagctctac taatttaaga tccgcctccc taattttcgg
    10861 gaagtcatgg cctgtgatct taatgtttac agtagtaata tcctgctgca ttctttttaa
    10921 aatatccaga cctcgtttcc ctcttacacg ctttatgggt tcaccggagt ctgcaatata
    10981 ctgcagctct tttataataa atttcggtac taatacctcg ccattaataa aaccagcttc
    11041 ggctacatct gcgattctac cgtctatgat aacacttgta tcaagtatca ttgacctaaa
    11101 agaacttttc tttgatgaat actttccacc aactctgtta tccgaatctg cgcctttttt
    11161 gattcctatt gttattcccg tgtatacaag aacaagaagc gctgcaacat ctgcgaaagg
    11221 tgcatattct gcaggtattg gtgcaatatt catgagttct ttaataagga cataaagtat
    11281 tgatgctgca actattccgc ctgcacttcc tgctgctgat ttaggagata tctcctccgt
    11341 acttggttcg atgataaata taaatacagc agcaagtgcg ccgaatagaa ttgttatttt
    11401 aatactgaaa tctacatttt ccgggttata gctgaaaaag aatcccagaa aagtaactaa
    11461 tgcaagaaca agaagcttaa ttaacatttc tgctaccccc aagattaaat tcctctcccc
    11521 aagacttata attaattaag aaagtaaatt attaactaat aggcatatat ccgttatgag
    11581 gaaaggtgaa aattattatg aattttttgc tgttattttt agaactaaac tgacaatttc
    11641 atcccttact cttatatacc tgctttcacc atacccggga ctttttgccc tgcccttaat
    11701 acttttatca gcaattatct caccagtatg tttatctata aggtttattc ttacggttac
    11761 cgagcttttt cccttgtcat ttcttctgag aaaccattcg cagtatttac atcctctgtt
    11821 gtaatttgta acctctcctt ttacaagtaa aatgtttttg cctgaaatat tagatctggg
    11881 tctgaaaaat tcagagcttg tacgggtaat ttctatattt ttaaattgag attcaagaga
    11941 cttggctacc atatctggta taatggtatt tgaat
//
LOCUS       DBNGSV010000241         5873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1635423, whole genome shotgun sequence.
ACCESSION   DBNGSV010000241 DBNGSV010000000
VERSION     DBNGSV010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1635423"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC159U_08870"
     CDS             <1..260
                     /locus_tag="AC159U_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337210.1"
                     /translation="SYAVQGDFIISEKKANIAFAGDRVVKLTSGGRGVDPEIMSSEFY
                     EQHGGIHLVLDRKQIKSALAGILKVTHWYRNIKDTADYSFV"
     gene            387..701
                     /locus_tag="AC159U_08875"
     CDS             387..701
                     /locus_tag="AC159U_08875"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3337211.1"
                     /translation="MKRLYTDQNSLMVGNIKNIMENNDIECIIKNFMLVGSVGELPPT
                     VCSTELWVINDEQYDQAKQILKEVFSEDKDKDPKWKCSSCGEMIEGQFTDCWNCGQGK
                     KG"
     gene            complement(1041..1322)
                     /locus_tag="AC159U_08880"
     CDS             complement(1041..1322)
                     /locus_tag="AC159U_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR3337212.1"
                     /translation="MASYRIFETDEFQKKLSKLEKKDYKFVYKKLLDYIYPQLKEEPA
                     YGKNIKKLRAYDPETWRYRTGNYRIFYSISDEEKIVYVLTIDKRKDSYK"
     gene            complement(1315..1557)
                     /locus_tag="AC159U_08885"
     CDS             complement(1315..1557)
                     /locus_tag="AC159U_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIR3337213.1"
                     /translation="MSKTITLRLEEKIYSKFKALADSDNRTMSNFIETSVLRYIETNN
                     LVDEYEMAEIESNDELNKSIKRAQKDMKQKKGKFVG"
     gene            2336..3043
                     /locus_tag="AC159U_08890"
     CDS             2336..3043
                     /locus_tag="AC159U_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3337214.1"
                     /translation="MNGFTILEVLIAILIMSIGFIAMSQMQYFSIQQKYKAEIGTTAT
                     NIIQFTSDADIAFAKKMQYLNSNVLIDVIAQRTPDYSYCDDGSDDNMDCGGCPCNPLS
                     AFMLDPTTIGTESSCAVIPITDYDPNELIYTDLDTCLSDLTDPSITTSESMVVVRTSN
                     LTQSAVDSTTVQTLELVYGVKSAKQFRETPGSVTIRDTLATQSFSVTAHIDNFNESGQ
                     ITLDAADWQTVRVPHLP"
     gene            3010..4266
                     /locus_tag="AC159U_08895"
     CDS             3010..4266
                     /locus_tag="AC159U_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3337215.1"
                     /translation="MADSKGATFTMKQDRDIKHKQGFTLVELLISIAVFTVIFTALML
                     AFQQQQRQFTFTQEAVNVDQTGRTALDFIASKLRNAGARQAKSYSVKFINGGSPNCTG
                     VTTNPGTKNSEPDCVQIYTWDKLRGHSVDVSGNEDYPSTPGDVTIITSDPFRVQVPKS
                     WSDASLIESGDSHLIGFWSRAILCDPTGTIDCNATPEQCTECGAILKVDADDVLSSGE
                     VIEFDNKDAIIQQNFQSADFADISTFIANFFIPRISSLTSEMTILEYYQFSVNQTTRS
                     LLYTTDPSIAPSAIAGGENSPGIVDLQFVFNLQNPDGTTSKVGVLDDTNAFTDFTDPS
                     LVESGSGPHISRTKDTTTVQVYLVVRSRLKPQLMSGNAPPPKNLPQIGDRSLISTSED
                     SLGEGYIYKIFTTTVYLRNLSREEVG"
     gene            4270..4875
                     /locus_tag="AC159U_08900"
     CDS             4270..4875
                     /locus_tag="AC159U_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337216.1"
                     /translation="MIEDSVYFKDKGIKNRHNDDSQKGIAIIIALITLLIISIMAVSI
                     SFVSNTDYSTMSSYKAGQEAFLAAERCTVEARKTFEIEGIETLVALQTIDKHDPLELT
                     LPNGAVCRSGPRFYDSGSDDSLPFVNLPEGVKSIQRSLKHTSLPSGGKSSPVAAALSF
                     EVLGKDSQDKDKDDKDDDINTGVHISVGIETFLPGGGSNVY"
     gene            4865..>5873
                     /locus_tag="AC159U_08905"
     CDS             4865..>5873
                     /locus_tag="AC159U_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337217.1"
                     /translation="MFIRKIRINRIIKGLKSKLPLIGIILLLVLSASLFLIEELHSDI
                     ITKNDVEDKNLLFTSVQPAKPNVGVLYDNSHSSGTSFGGGQVGRWNNFSPIAHCTKII
                     EGKGGDPDIPVPKVCFNEGLSTDECRATAAHCVANATGLTREQPDGSFLGCGSSECST
                     SIWGTCDDPDDFSNFLDCIEANYDSSVVASAYNLAVPIACDTLSIALPSAPIITTKAT
                     VETACNDDATGTERAHAAAGIENLTNYLANQINSTTFPLTCGGSICGDLDTDDDGDID
                     LDDLDTSGDGLFTILDSIDPSCNVDGGDFVTYMECMGGTCANNSVSGSICEGDVSKQL
                     RV"
BASE COUNT         1847 a         1061 c         1162 g         1803 t
ORIGIN      
        1 caagttatgc cgtgcaggga gattttatta tctcagagaa aaaagctaat attgcctttg
       61 caggagacag ggtagttaag cttacaagcg gcggcagggg agtagatcct gaaataatgt
      121 catcagaatt ttatgagcag cacggtggta ttcatcttgt gcttgataga aagcaaatta
      181 aatcagcgtt agcaggaata ctgaaagtca cccactggta cagaaacata aaagacacgg
      241 ctgattattc atttgtgtag ttttctataa ctcttgcttt atattctgcc agtctttata
      301 aaataaactt tcataagata ttagtggagc tttgtttgaa atattgctga atatgtttta
      361 cataattgtt atctaataga agaagaatga aaaggttata cacagatcaa aacagcctta
      421 tggttgggaa catcaaaaat attatggaaa acaacgacat agagtgcatt ataaaaaact
      481 ttatgctcgt tggctctgta ggcgagctgc cccccacagt gtgttcaaca gagctttggg
      541 ttataaatga cgaacaatat gatcaagcca agcagattct aaaagaagtc ttttctgaag
      601 ataaagataa agacccaaaa tggaagtgca gtagctgcgg tgagatgatc gaaggacagt
      661 ttacagactg ctggaattgc gggcagggta agaaaggctg atcaaaaatt gtaaaaaatt
      721 tatttgtctt caatccatcc tttcaagtac attgccaaca acataaaacc ccacatagca
      781 caaaatatcc ctgaataaat catatgctct ggcttatatt tgtcgaaata atctacttca
      841 tcatctggta acaaattagg taatattttt tgcagattct aatccgtatc atctagctgt
      901 ctttttcgta tcttttttga tgtattagta ttatttgatg tttttttata tatatatgct
      961 gtaatagata tacaagaacc caggataatg aaaattaatg gtacaaataa aaacatgttt
     1021 gatatttaga ttaaataatt ttacttatac gaatccttcc tcttatctat cgtaagaaca
     1081 taaactattt tttcttcgtc acttattgaa taaaagattc tatagtttcc tgttcggtat
     1141 cgccaggttt ctggatcgta ggctctgagt tttttaatgt ttttgccgta agcgggttct
     1201 tcttttaact gggggtagat atagtctaaa agttttttgt atacaaactt ataatctttt
     1261 ttttcaagct tacttaactt tttttgaaat tcatcagttt caaaaatcct atagctagcc
     1321 aacaaacttt cccttcttct gcttcatatc tttttgcgca cgctttatac ttttgtttag
     1381 ttcatcattt gattcgatct ctgccatttc atattcatca actaaattgt tggtttctat
     1441 ataacgaagc acagaagtct caatgaagtt tgacatcgta cggttgtcac tatcggcaag
     1501 tgctttgaat ttactgtaaa ttttttcttc aagtcttaag gttatagttt tagacatttt
     1561 aacctcgtat aattagaata cattgtattt atttgtaata aaaagtatac agtagggctt
     1621 taatagtgca aattgaaaaa cactatagat tcgcaacatt aatcgggatg tattatcagg
     1681 tagttctggg taccttatcg gatgtattcc gaacaaagat aatggaactc aataacactg
     1741 tatttattaa taatctgaaa attacactac aaactttcaa tatttatcca tacgatatga
     1801 atgagatgtt attgatatgt tgattcaatt gttgatttta attcaggaac aatacataga
     1861 ttatacaaat aaacaataaa aaaagcgagg cttgaaattt tatccaagcg tatctttttt
     1921 agattaatat ctattgtcta aagttaatta actaatctta gaatcattaa gtgatcttag
     1981 tattgctgca aagagtccga gaactatggc agcaagaagt attccctact gacccttggt
     2041 gggaatgatg acagttggtc tgcctttaca agatggatga tgtatttcat catatttatt
     2101 tgtatcaaat ctttgtaata agaatatgaa aagcgtgcaa aaccttgcac gctatgcgca
     2161 acaatctgca tgcttcaata tagtttaaac aatattacaa ctaacataat ataatgattt
     2221 taatatttta cattaaatct atgaactggt atgttaattg taataataaa attggtctag
     2281 tttcttatat tctttaattt tcatatagta gaggggagta ataattaaaa cgaatattaa
     2341 tggattcacc attctcgaag tcctgattgc aatacttatt atgagtatag gctttattgc
     2401 tatgtctcag atgcagtatt tttccattca acaaaaatat aaagccgaaa ttggaacaac
     2461 tgccacaaac ataatacagt ttacaagtga tgcagatata gcatttgcaa aaaaaatgca
     2521 gtatttaaac tctaatgtac tcattgatgt catagctcag cggacccctg actactcata
     2581 ttgtgacgat gggagtgatg ataatatgga ctgcggcggg tgcccatgta atcccctctc
     2641 tgcgttcatg ctggacccca caactattgg aactgagtca agctgtgcag taataccaat
     2701 tacagattat gatccaaatg aactgatata caccgatctc gatacatgtc tatcagattt
     2761 aaccgatccg tcaataacaa caagtgaatc tatggttgtt gtaagaacat ctaatcttac
     2821 acaatctgct gtagacagta caactgttca gactttagag cttgtatatg gcgtaaaatc
     2881 tgccaaacaa tttagggaaa ctcctggttc agttactatt cgcgatacgc tagctacaca
     2941 gtctttttca gtaactgctc atatagacaa ttttaatgaa tccggtcaga taacattgga
     3001 tgccgcagat tggcagacag taagggtgcc acatttacca tgaaacaaga tagagatatc
     3061 aaacacaagc agggtttcac acttgtggag ctcctaattt caattgcagt atttacggtg
     3121 atttttaccg cattgatgct ggctttccag cagcagcagc gccagtttac ttttacccag
     3181 gaggccgtaa atgtcgatca gacaggaagg acggcccttg attttatcgc aagcaaatta
     3241 agaaacgcag gtgcaaggca ggcaaaaagc tattctgtaa aatttattaa tggtggtagt
     3301 cccaactgta caggggttac aacaaaccct gggactaaaa attctgaacc tgactgtgtt
     3361 cagatctata catgggataa gttaagagga catagtgttg acgtatccgg aaatgaagat
     3421 tacccttcta cacccggaga tgttactata attacaagtg atccattcag ggtacaggtt
     3481 cccaagtcct ggagtgatgc aagccttata gaatctggtg attctcattt aataggcttc
     3541 tggtcccggg caatactttg tgatccaacg ggtactatag actgcaatgc aactccagaa
     3601 cagtgtacag aatgcggggc tatcctgaaa gtcgacgctg atgatgtgct ctcttcgggt
     3661 gaggttatag agtttgacaa taaagatgca ataatacagc agaatttcca aagtgctgac
     3721 tttgctgata tcagcacttt tatcgctaat ttctttatac ccagaatatc ttcacttacc
     3781 tctgaaatga cgattctcga atactatcaa ttctcagtga atcagaccac ccgttcgctt
     3841 ctttacacaa ccgatccttc aattgctcct tctgccatag caggtggaga aaactctccg
     3901 ggtatagttg acctgcagtt tgtttttaat ctgcaaaacc cagacggtac tacgagtaaa
     3961 gttggtgtgc ttgatgacac caatgcattt actgatttta ccgatcccag cctggttgaa
     4021 tcgggaagcg gtcctcatat aagccgcaca aaggatacaa caaccgttca ggtctatttg
     4081 gttgtaaggt caaggctaaa accccagctt atgagcggta atgctcctcc gcctaaaaat
     4141 cttcctcaaa tcggagacag gagcctaata tcgacaagcg aagattcgct tggtgagggg
     4201 tatatttaca aaatatttac aacaacagtg tacttgagaa acttatctag agaagaggta
     4261 ggctaaacaa tgattgaaga ctctgtttat tttaaggata aaggcataaa aaatcgtcat
     4321 aatgatgatt ctcaaaaagg tatagctata ataattgcac taattaccct gcttatcata
     4381 tctatcatgg ctgtttcgat atcatttgtt tcaaatacgg attattctac aatgagcagt
     4441 tataaagcag ggcaggaagc atttcttgca gccgagcgct gcacggttga agcgagaaag
     4501 acgtttgaga ttgaaggtat agaaactctg gtagccctgc agacaataga taaacatgat
     4561 ccgcttgaac ttacacttcc aaacggcgca gtatgcagat cgggaccaag attttatgac
     4621 tctggttcag atgattctct tcctttcgtt aaccttccgg aaggagtaaa gagcatacag
     4681 aggtctttaa agcacacatc cctgccatct ggggggaaga gctcgcctgt ggctgcagcg
     4741 ttgtcatttg aggtattagg taaagactcg caagacaaag ataaagatga taaagatgat
     4801 gatataaaca ccggagttca tatttcggtt ggaatagaaa cttttcttcc aggagggggg
     4861 agcaatgttt attagaaaaa ttaggattaa tcgaattata aaaggtttaa aatctaagct
     4921 accgcttata ggtattatat tattgctcgt tctttcggca agcctgtttt tgatagaaga
     4981 attgcatagt gatataataa ctaaaaacga tgtcgaggac aaaaacctcc tctttacatc
     5041 agttcagcct gcaaagccta atgtgggtgt cctttatgat aactctcatt cctcgggcac
     5101 tagcttcgga ggcgggcagg taggccgctg gaataatttt agccctattg cacactgtac
     5161 aaaaattatt gagggaaaag gtggcgatcc tgatattccg gttccaaaag tatgttttaa
     5221 tgaaggacta agcactgatg aatgtcgcgc aactgctgcg cactgcgtgg caaatgccac
     5281 gggtttgaca agggagcagc cagatggaag ttttcttgga tgtggcagca gcgaatgttc
     5341 tacatccatc tggggcacat gcgatgatcc cgatgatttt agtaattttt tagactgcat
     5401 agaggcaaac tatgacagtt ctgttgtggc ttctgcctat aacctcgcgg ttcccatagc
     5461 atgtgataca ttatctatag cacttccttc tgcgcccata ataactacta aagcgacagt
     5521 ggaaactgcg tgcaatgatg atgcgactgg cacagaaagg gcacatgctg ctgccggaat
     5581 agaaaatctt actaattacc ttgccaatca aattaattct actacatttc cacttacatg
     5641 cggaggctcg atatgtggtg acctcgatac agatgatgac ggtgatatag atttggacga
     5701 tctggataca agcggtgacg gattatttac gattttggac agcatcgacc catcatgcaa
     5761 tgtcgacggt ggggactttg tgacttatat ggagtgcatg ggtggaacat gtgcaaacaa
     5821 cagtgtatct ggttcaatat gtgaaggcga tgtatccaag cagttaaggg tac
//
LOCUS       DBNGSV010000242        10531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1081681, whole genome shotgun sequence.
ACCESSION   DBNGSV010000242 DBNGSV010000000
VERSION     DBNGSV010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1081681"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="AC159U_08910"
     CDS             <1..580
                     /locus_tag="AC159U_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3337218.1"
                     /translation="LVKPSLEVHALISASENMTGGGAYKPDDVVTAMNGKTMEIVNTD
                     AEGRVVLSDALSFAVELGVDEIVDLATLTGACLVALGTYTAGVMGNDQLVINRILKAA
                     NTVGEKIWQLPMDDELRKDIESDVADVKNVGSRYGGAITAGMFLEHFVDDTPWAHIDI
                     AGPTFIEKGGEFYPKGATGFGVQTILQYLLER"
     gene            624..2273
                     /gene="sppA"
                     /locus_tag="AC159U_08915"
     CDS             624..2273
                     /gene="sppA"
                     /locus_tag="AC159U_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3337219.1"
                     /translation="MTLYDSIINSLFNSNLLEIELEGDIPEETESIPIPFIQIKKKPT
                     VWELEKVLTHVSENEYIKCVILKIKDLHIGLARSESIRRKLFEVRKSGVKVFTYLEGS
                     GNAEYLIASASDKIIVPPWSTLNLVGLKAEVVFLKDAFEKYDIEPHFKSVGEFKSAVE
                     TFTSNKMSKAHREMLDSLLGDLFSQLALSISEGRSKSPASIEKIIDDGPYIPDEAQKN
                     GLIDSIGYEHEFDKEIEILLDHKIRKVEIDRYIKLLGAKDRLRAFISFFKNNTFNIGL
                     LTDTGMITPGDSRGRGKSKNIGHNTTIKALREVYKSNKLKALVYRVSSPGGSGLSSDL
                     ICRELERLSKKIPVIISMSDVAASGGYLIALGGNRIIADPFTLTGSIGVFGGKINVAN
                     FLKRFGINIESVNKGKNALMFSPTKGFSEAESKKITSMMKDLYDKFITRVSEARNLTK
                     AETEKNAKGRVWTGNQAKELKLIDEFGGIYDCIKLAASQANIDISKVIVKTVKKPKVL
                     DLSTIGKSFGFTSVYNEYTEKLSMLANEDVKTIMPFILKIK"
     gene            complement(2279..3199)
                     /locus_tag="AC159U_08920"
     CDS             complement(2279..3199)
                     /locus_tag="AC159U_08920"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3337220.1"
                     /translation="MTEMPYKIWFNGKLVDWKDANVHVLTHTLHYGLGVFEGIRAYEC
                     KDSKTAIFRLDEHVERMFKSAHIAMMKIPFSKKQITEAIVHTVEENELVSCYIRPLAF
                     IGDGVMGVHPKDNPVSVIIAVWPWGAYLGDDGINNGIRAKVSSFTRMHVNASMTKAKI
                     CGNYVNSIYAKLEVTSLGYDEAILLDSDGYVAEGSGENIFIVRNGKLITPPLTSTLEG
                     ITRNTVIEIAQDEGYEIDYQRFTRDEFYIADESFMTGTAAEVTPIRELDDRAIGTGAP
                     GEITKNIQKIFFDSVQGKNKKYKKWLTYLK"
     gene            3324..3809
                     /locus_tag="AC159U_08925"
     CDS             3324..3809
                     /locus_tag="AC159U_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR3337221.1"
                     /translation="MRNKNNNSDIIQIKKYSNRRLYHSAQKRYINLQQISDLIKSGNT
                     IKVIDTQSKEDITKQILIQTILESEKNKQDMLPVPFLHKLIRYGNKLSNSYLDKSFIM
                     MMQPYLYENNNTDNNDSLKYSKFANTSSSKLKPKNRNDDLLKIKQKMEELEQQIRNIS
                     N"
     gene            complement(3810..4904)
                     /locus_tag="AC159U_08930"
     CDS             complement(3810..4904)
                     /locus_tag="AC159U_08930"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF048136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3337222.1"
                     /translation="METVTKTITIIFLFLTSYIPSCADTGDIILPKNFSIDTYAEKLK
                     GPRFMALSPDNVVFVTEIRSGHVMALPDINKDGKTDEYLIKAGRLRYPNGIAFYKNWL
                     YVGETHQIVRYQYFGYKNDLGKKEVIVPNLPTGGQGTKTILFGKDNKLYVSIGSSCNA
                     CKEKDKRRAAVMQYNADGSNGRLYAEGLRNSVGIISHPSTGQIWSTDNGTDWLGDDLP
                     PEEINILEDGKHYGWPYCYGKRIPDPNMGNENFCRNTMSSTIDLQAHSAALGLRFYNG
                     NQFPKEYHGDLFVAYHGSWNRSTPTGYKVVRIKTKNNKPVLIEDFASGWLKGDKRTGR
                     PVDVLVANDGSLLISDDREGKIYRISYEKL"
     gene            complement(4912..5583)
                     /gene="lipB"
                     /locus_tag="AC159U_08935"
     CDS             complement(4912..5583)
                     /gene="lipB"
                     /locus_tag="AC159U_08935"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3337223.1"
                     /translation="MQEFKILSLGITNYSKALDIQLKLLDKRKNNLIPDTLILLEHPP
                     TITIGRAGNLNNLLVSEEHLKNNGIHFEKISRGGDITFHGPGQIVGYPIIDLNNLQKD
                     IHKYLRALEYLIVDVLKKFNIKASPFKGITGVWANGKKIASIGIGVKRWITYHGFALN
                     IDNDLEYFDMIIPCGLNKVRMTNIQNESDLQNITMEEINKYIIDSFSKTFNMNYSGTL
                     DLKTG"
     gene            complement(5632..7065)
                     /gene="lpdA"
                     /locus_tag="AC159U_08940"
     CDS             complement(5632..7065)
                     /gene="lpdA"
                     /locus_tag="AC159U_08940"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3337224.1"
                     /translation="MDKYDLTIIGSGPGGYVAAIRAAQLGRKVSIIERDYPGGVCLNW
                     GCIPSKAILKCAEVYQNFLHSEDYGITTKGLSFDYSKVIQKSRNASSALTKGVEFLMK
                     KNKIDLFKGTGKLIDKNKVGIIGEKDEIELVSNNILICTGSVPRVIPGIEVDGKMIMT
                     SDEAIKSTDFPKSVVIIGGGYIGAEFAYVYNSFGSKVTIIEMAENLIPGADKEVAKTL
                     EKIFKRSGMDVFTSTKYKSYKKTKTQVEVVVENVKDGKEQAIKSSQMLIAIGRKAVAN
                     DAPTSYAYYKESKDIGLKSLNIELNNHGFIKTNDDYETTCSNIYAIGDVIGPPLLAHK
                     ASEEGVVAVEKMNGIDSKVHYNTIPSCVYCQPEIGSVGYTEEQIKELGIKYDVGKFPF
                     KAAGKAVAVGDTEGFVKILTDARTGEVLGSHIIGHGATEMIGELTIAKALESTAFEIA
                     ETSHAHPTLSEAIMEAALGTMGRTRNM"
     gene            7215..7517
                     /locus_tag="AC159U_08945"
     CDS             7215..7517
                     /locus_tag="AC159U_08945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3337225.1"
                     /translation="MALWFEQKAPDMAKGWWEFYNAAEGEGKLDSKTKAAVALATAIT
                     GRCPHCTGSRIKKALAAGFSKEEIAEVIMEVALVASGTELFWAKEVYDKYLLDEEK"
     gene            7684..10368
                     /gene="aceE"
                     /locus_tag="AC159U_08950"
     CDS             7684..10368
                     /gene="aceE"
                     /locus_tag="AC159U_08950"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR3337226.1"
                     /translation="MSVDKNIDNEKDLLEEVENKEWLQSLDYVLNTYGSERVNRLLRK
                     LHIHARKAGVNIPFTANTPYINTIPLDKQENFPGSIEIERRIRSYIRWNAMAMVVRAN
                     RHSDGIGGHISTYASAATLYDIGFNHFFRARTEKQEGDIIYFQGHASPGIYSRAFLEG
                     RLDIEHLENFRNEVSSDKGLSSYPHPWLMPEFWQFPTVSMGLSPIMAIYQARFNRYLE
                     DRELKKETDSKVWAFLGDGETDEPETLGAITLASREELDNLIFVVNCNLQRLDGPVRG
                     NGKIIQELEAIFRGAGWNVIKVIWGNDWDPLLAADKDDILVDRMQRAVDGDYQKYIVE
                     PGSYIREHFFGTDPRLKKLVEHYTDEQLERLRRGGHDSEKVYTAYKAAVDHKGSPTVI
                     LAKTIKGYGLGESGEGKNITHQQKKLNEDELKEFRTRFGIPISDEDVSKAPFYKPDEN
                     SKEIIYLKNCRERLGGAIPKRNTKITKFKMPSDEIYNEFIDGSGDREVPTTMAFVRIL
                     SKLLRDKNVGDLIVPIVPDEARTFGMEALFRQVGIYSHVGQLYEPVDKETLLYYKEAL
                     DGQILEEGITEAGSMSSFIAAGTSYSTHGINTIPFFIYYSIFGMQRIGDLVWAAGDMK
                     CKGFLIGGTAGRTTLAGEGLQHQDGHSHLLAYPVPNLLAYDPAYAYEIAVIIKNGMER
                     MYVKGDDVFYYLTVMNEGYIQPPMPKGSEEGIIKGIYKLNSSDNKDSKLKVSLLGSGT
                     ILNESIKAKKILEEEYELSVDLFSVTSYKELYKDCLETERWNMLHPSEISKSTFLSKV
                     IDHDSDIIVAASDYVKTLPESISSWLPAKCISLGTDGFGRSASRDDLRSFFEVDYRYI
                     VLASLYGLSKDKKVKTELVQKACKKFDIDTTKDNPFIS"
     gene            10385..>10531
                     /locus_tag="AC159U_08955"
     CDS             10385..>10531
                     /locus_tag="AC159U_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3337227.1"
                     /translation="MEFKLPELGEDIEGGDIIAIAVKKGETVKKEQTLMEIETDKAVI
                     ELPSP"
BASE COUNT         3543 a         1786 c         1896 g         3306 t
ORIGIN      
        1 tttagtaaag ccatcattag aagtacatgc gcttatatca gcctcagaga atatgacagg
       61 aggcggagct tataaacccg atgatgtagt tacagcaatg aatggaaaaa ccatggaaat
      121 tgtaaacaca gatgctgagg gaagggtagt gctgtctgat gcattatcat ttgcagtgga
      181 gctgggtgtg gacgaaatag ttgaccttgc tactcttact ggtgcatgct tagtggctct
      241 aggaacatac acagctggtg ttatgggaaa tgatcaatta gttataaata gaatccttaa
      301 agcagcaaat actgtaggcg agaaaatatg gcagctccca atggatgatg aacttagaaa
      361 ggatattgaa agcgatgtag cagatgtaaa gaatgttgga agcagatacg gcggagcaat
      421 tactgctgga atgttcctgg aacattttgt agatgatact ccatgggcac atatagatat
      481 tgcaggtcct acatttatag aaaaaggtgg agagttttat ccaaaaggtg caacaggttt
      541 tggcgtacag acaatactgc agtatttatt agaaagataa aaacttaaga gcgttcctaa
      601 aataacttaa cttattaaaa tatatgactc tgtatgattc tattataaac agcttgttta
      661 attctaacct tctagagatt gagctagagg gtgatattcc tgaggaaaca gaatccatac
      721 ctattccatt tattcagatt aagaaaaaac ccacggtatg ggagcttgag aaggtattga
      781 ctcatgtatc tgagaacgag tacataaaat gcgtaatcct aaaaattaaa gatcttcata
      841 ttggtcttgc aagatctgag tctataagaa gaaaactttt tgaagtcaga aaatccggag
      901 taaaagtgtt tacttatctt gaaggctcgg gcaatgccga atatttaata gcctcggcat
      961 cagacaaaat aattgtgccg ccctggtcta ctcttaatct tgtgggttta aaagcagagg
     1021 tagttttctt aaaagacgca tttgaaaaat atgatataga acctcatttt aaatccgttg
     1081 gggagtttaa gagtgcagta gaaacattta catcaaataa gatgtcaaag gctcacaggg
     1141 aaatgctgga ctctttgctg ggtgatttat ttagtcagct tgctctgtca atttctgagg
     1201 gaagaagtaa atcccccgca agtattgaaa aaataattga tgacggtcca tacatacctg
     1261 atgaagcaca aaaaaacggt cttatagatt ctataggcta tgaacatgaa tttgataaag
     1321 aaattgaaat attgctagac cacaaaataa gaaaggttga gatagataga tatataaaac
     1381 tgttgggagc taaggacagg ttgagggctt ttatatcatt ttttaaaaat aatacattca
     1441 atatcggtct cctaacagat acaggaatga ttactccagg ggatagtaga ggcaggggaa
     1501 agtcaaaaaa tattggccat aatacgacta tcaaggcact aagagaggtt tataagagca
     1561 ataagctaaa agcacttgta tacagggtat caagccctgg aggttctgga ttatcctcag
     1621 accttatatg cagagaactg gaaaggttat ctaaaaaaat accggtaatt atttctatgt
     1681 ctgatgttgc agcatcaggt ggatacttaa tagcactcgg aggaaataga ataatcgccg
     1741 atccttttac tcttacaggt tctataggag tatttggcgg aaagataaac gtagctaatt
     1801 ttctaaaaag gtttggtata aacatagaat ctgttaataa agggaagaat gctcttatgt
     1861 tctcacccac aaaagggttt agtgaagcag agtctaaaaa gataacctca atgatgaaag
     1921 acctttatga caagtttatt acaagagtat ctgaagccag aaatcttaca aaggcagaga
     1981 ctgaaaagaa cgcaaaggga agggtatgga cgggtaatca ggcaaaagaa cttaagctta
     2041 tagatgaatt tggcggaatt tatgactgta ttaaactggc agcatcacaa gccaatattg
     2101 atataagtaa ggtaatagtg aaaactgtaa aaaaaccaaa ggtgttagac ctttcaacaa
     2161 ttggaaaaag ctttggattt acctctgttt ataatgagta tactgaaaaa ttaagcatgc
     2221 ttgcaaacga agatgttaaa accataatgc catttatcct taaaattaaa taggattctt
     2281 atttaagata agtaagccac tttttatatt ttttattctt tccctgaact gaatcgaaga
     2341 atatcttttg tatatttttt gtaatctctc caggcgcacc tgtaccaata gccctgtcat
     2401 caagttctct tataggtgtt acttcggctg ccgttcccgt cataaaagat tcatctgcaa
     2461 tatagaattc atctcttgta aacctttgat aatcaatttc atagccctca tcctgtgcta
     2521 tttcaattac agtatttctt gttatacctt cgagggttga ggttaacgga ggagtgataa
     2581 gttttccatt tctaactata aatatattct caccactgcc ttcagcaaca tagccatcag
     2641 aatccagcag aatagcttca tcataaccaa gtgatgttac ttcgagcttg gcatagatgg
     2701 aatttacata attgccacaa attttagctt tagtcattga tgcatttaca tgcattcttg
     2761 taaaagagga aacttttgcc cttattccgt tattaattcc atcatcacct aaataagcac
     2821 cccatggcca aactgctatg ataacactta cggggttgtc ctttgggtgt acacccatta
     2881 ccccatctcc aataaaagcc aggggtctta tatagcatga tactagttcg ttttcttcaa
     2941 ctgtatgaac aattgcctct gtaatctgct ttttagaaaa aggtattttc atcatcgcaa
     3001 tatgtgcaga tttaaacatc ctttctacat gttcatccaa tctgaatatc gcagtctttg
     3061 agtctttgca ttcataagcc cttatacctt caaaaacacc aagaccatag tgaagagtat
     3121 gagtcagtac atgtacattt gcatctttcc agtcaacaag tttgccgtta aaccatattt
     3181 tatagggcat ttcagtcatt tatcatctcc atactgtgtt aaaaaacttc tattataccc
     3241 aaattaatat tttgttaaca atattcataa aataaatatc atatcttgac ttagggtatg
     3301 ttttttgaat aatgatcttg caaatgcgaa ataaaaataa taacagtgat attattcaaa
     3361 taaagaaata cagtaataga agattatatc atagtgcgca aaagagatac atcaacctac
     3421 agcaaatatc tgatttaatt aaaagtggta ataccattaa ggttatagat actcaaagta
     3481 aagaagatat tactaaacaa atacttattc aaactatatt ggaaagtgaa aagaataaac
     3541 aggatatgct tccggttcct tttttacata aacttataag atatggtaac aagcttagta
     3601 atagttattt ggataaaagc tttataatga tgatgcagcc atatctttat gaaaataata
     3661 atactgataa taatgattct ttaaaatact ctaaatttgc aaataccagc tcaagcaaat
     3721 taaagcctaa aaacagaaat gatgacttat taaaaataaa acaaaaaatg gaagaacttg
     3781 agcagcaaat tagaaatata agtaattagc tataattttt cataactaat cctgtatatc
     3841 ttaccttctc tatcatctga gattaaaaga ctgccgtcat ttgcaactaa tacatcaact
     3901 ggtctgcctg ttctcttatc acctttaagc cagcctgatg cgaagtcttc tattaaaact
     3961 ggtttattat ttttagtttt tatcctgaca actttataac cagtgggcgt tgatctgttc
     4021 catgagccat gatatgcaac aaaaagatcg ccatgatatt cttttgggaa ttgattgcca
     4081 ttatagaatc gtaatccaag ggcagctgaa tgcgcctgaa gatcaatagt agatgacatt
     4141 gtatttctac aaaaattctc attacccata ttaggatctg gaattctttt tccataacaa
     4201 taaggccatc catagtgctt tccatcttct aatatgttta tctcttctgg cggcaaatca
     4261 tctcctaacc agtcagttcc attatcagta ctccatattt gtcctgtgga tggatgagag
     4321 attattccta cagagtttct aagtccttca gcatacaacc tgccattact accatccgca
     4381 ttgtactgca tgacagctgc tcttcttttg tctttttcct tacatgcatt acaagatgag
     4441 cctatagaca catataattt attatctttt ccaaatagta tagtttttgt tccttgtccg
     4501 cctgttggta gattgggtac tataacttct ttttttccaa gatcattttt atagccaaaa
     4561 tattgatatc ttacaatctg atgagtttct ccaacataaa gccaattctt ataaaaagca
     4621 atcccgttgg gatatctcaa cctaccagct ttaattaaat attcatctgt tttaccatct
     4681 ttgtttatat ctggtagggc cattacatga cctgaacgta tttcggtaac aaaaacaacg
     4741 ttatccggac taagagccat aaacctggga ccttttagct tttcagcata agtatctatg
     4801 ctaaagtttt tgggaagtat gatatcacct gtatcggcac aggatggtat ataagaagtc
     4861 aaaaataaaa atataatagt aattgttttt gttacagttt ccatatgttt attaacccgt
     4921 tttcaaatct aaagttccgg aataattcat attaaaagtt ttggagaatg aatcaataat
     4981 atatttatta atttcctcca ttgttatatt ttgtaaatct gattcatttt gtatattggt
     5041 cattcttact ttatttagtc cgcatggaat aatcatatca aaatattcaa gatcattatc
     5101 gatgtttaat gcaaacccgt gataggttat ccatctcttt actccgattc ctatagaagc
     5161 aatttttttt ccatttgccc atactcctgt aattccctta aaaggagaag cttttatatt
     5221 aaatttctta agcacatcaa caattaaata ctctaaagct cttaaatatt tgtgaatatc
     5281 tttttgaaga ttatttaaat ctattatagg atatcctaca atctgtccgg ggccatgaaa
     5341 ggtgatatca ccacctctgc tgatcttttc aaaatgtatt ccattatttt taagatgttc
     5401 ttcggatact aaaagattat ttaaattacc tgctctgcct atagtaattg taggagggtg
     5461 ctctagtagt atgagggtat ctggaataag gttgttcttt cttttatcta gaagttttaa
     5521 ctgaatatcc aaagccttac tataatttgt aataccaagg cttaggattt taaattcttg
     5581 catataataa ttatactaaa attattaccg ggtgtcttca gagcaatata gctacatatt
     5641 tcttgttctt cccatagtgc ccaaggcagc ttccattata gcctcagaaa gagtagggtg
     5701 agcatgcgaa gtctcggcta tctcaaaagc agtagactct aaagcttttg caattgtaag
     5761 ctctcctatc atctctgtgg ctccatgccc aattatatgt gaacccaaaa cttcacctgt
     5821 tcttgcatca gttaaaatct ttacaaaacc ttcagtgtca cccactgcta cggcttttcc
     5881 tgcggctttg aaagggaatt tgcctacatc atatttaatt cccagttctt tgatctgttc
     5941 ctctgtatat cctacagagc caatctctgg ctggcagtac acacagcttg gtatagtatt
     6001 ataatgaacc ttagaatcta taccgttcat tttttcaact gcaacaacac cctcttctga
     6061 tgctttatga gcgagtaacg gtggtcctat gacatcaccg atagcataga tattgctgca
     6121 agtagtctca taatcatcat tagtttttat aaaaccatga ttatttaact caatatttaa
     6181 tgatttaaga cctatatcct ttgattcttt gtaatatgca taagaagtag gggcatcatt
     6241 tgccacagcc ttcctgccta ttgctataag catttgcgat gatttaattg cctgctcttt
     6301 gccatctttt acattctcaa ctactacttc tacctgtgtt ttagttttct tatatgattt
     6361 gtattttgtg ctcgtaaata catccatacc agatctctta aatatcttct caagagtctt
     6421 tgctacttct ttatctgctc ctggaattag attctctgcc atttctatta ttgtaacctt
     6481 gctaccaaat gagttataaa catatgcaaa ctctgcacct atataaccac cgcctataat
     6541 aacaaccgac ttaggaaaat cagtagattt tatggcctca tcgcttgtca ttatcatttt
     6601 tccatctact tcaattcccg gtatgactct tggcactgaa cctgtacaaa taagtatgtt
     6661 attagaaaca agttcaattt catctttttc accaattatt ccaaccttgt ttttatctat
     6721 taattttcca gttcctttaa atagatctat cttgtttttt ttcatcaaaa actcaacgcc
     6781 tttggtaagg gcagatgaag cgttcctgct tttttgtatt accttggaat aatcaaatga
     6841 aagtcctttg gttgtgattc cataatcctc agagtgtaaa aagttttgat aaacttcagc
     6901 acatttaagt attgcctttg atggtatgca tccccagtta agacaaactc caccgggata
     6961 atctctttct ataattgaaa ccttccttcc aagctgagct gctcttatag cagctacata
     7021 gcccccaggg cctgatccga ttattgttaa gtcatattta tccatagcgg gtaataatat
     7081 aaaagtaatt acaaaatggt caataatttt tgtataaacc tacttttgtt gaatagatat
     7141 ataaaaaata actatcttat ttataatatt ttgttataaa ttaagtataa gtaaatcact
     7201 aggaggtaat acaaatggca ttatggtttg aacaaaaagc gcctgatatg gcaaaaggat
     7261 ggtgggagtt ttataatgca gctgaaggag aaggaaaatt agactcaaag acaaaggcag
     7321 cagtagctct tgctacggca attacgggta gatgccccca ttgcacgggt tccagaataa
     7381 aaaaagccct ggctgcagga tttagcaaag aagagattgc tgaagttata atggaagtag
     7441 cgcttgtggc ttcaggcaca gaactttttt gggcaaaaga agtttatgat aagtatctat
     7501 tagatgaaga aaaataatgt tattttaaat attgaatcca tatagatttt gttcggatca
     7561 taatatttaa tagttacagc taaataagag ttaagatata ttagttctta actctatatt
     7621 tatacattta attatccaag taaatatcaa tcagatatta ataagtataa taacatttga
     7681 gatatgtctg tagataagaa tatagataat gaaaaagacc ttcttgaaga agtagaaaat
     7741 aaagaatggc tgcaatctct agattatgta ttaaatacat acggaagtga aagagtaaac
     7801 aggcttctca gaaaactaca cattcatgcc agaaaagccg gcgtaaatat tccatttaca
     7861 gcaaatactc cttacattaa tacgatccct ttggacaaac aggagaactt tcccggaagc
     7921 atcgaaattg aaagaagaat tagaagctat attcgctgga atgccatggc tatggtagta
     7981 agagcaaata gacatagtga tggaataggc ggacatatat caacctacgc atctgcagct
     8041 actctttatg atatcggctt taatcacttt tttagagcaa gaactgaaaa acaggaagga
     8101 gatataatat attttcaggg gcatgcatct cctggcatat attctagagc atttcttgaa
     8161 ggaagactgg atatagaaca ccttgaaaac tttagaaacg aagtaagttc tgataaagga
     8221 ctttcctctt atccccaccc atggcttatg cccgagtttt ggcagttccc aacagtttct
     8281 atgggactta gtcccatcat ggcaatttat caggccaggt ttaatcgtta tttagaggat
     8341 agggaactta aaaaagaaac agattccaaa gtctgggcat ttcttggtga tggtgaaaca
     8401 gatgagcctg aaacacttgg tgctattacc ctggcttcaa gagaagaact tgataacctt
     8461 atatttgtag ttaactgcaa tttacagaga ctcgacgggc ctgttagggg aaacggcaag
     8521 ataatacagg aactagaagc tatattcagg ggagcagggt ggaatgtaat aaaagttatt
     8581 tggggaaatg actgggatcc cctattagca gctgataagg atgatattct tgtagacagg
     8641 atgcaaagag ctgttgatgg agactatcaa aaatatatag ttgaaccggg ctcatacatt
     8701 agagaacatt tctttggtac cgatccgaga ttaaaaaaac ttgtagagca ctataccgat
     8761 gaacagctag aaagactccg aagaggaggg cacgattcag aaaaagtata tacagcttat
     8821 aaagcggcag ttgatcataa aggttctcca actgtgatac tagcaaaaac tattaaaggt
     8881 tatggtcttg gagagagtgg agaaggaaaa aacatcactc atcagcaaaa aaagcttaat
     8941 gaagatgaat taaaagaatt tcgtactaga tttggaatac caatatcaga tgaagatgtt
     9001 tcaaaagccc ccttttataa accagacgaa aacagtaaag aaataatata tttaaaaaat
     9061 tgcagggaaa gacttggagg cgcaattcca aaaagaaata ccaagattac taaattcaag
     9121 atgccttctg acgagattta taatgaattc attgatggtt ctggagacag agaagttcct
     9181 acaacaatgg catttgtaag aatattatca aagcttctaa gagataagaa tgttggtgac
     9241 ctcatagttc ctattgttcc tgatgaagcg agaacatttg gtatggaagc tctttttaga
     9301 caggtgggaa tatattcaca tgtgggccag ctttatgaac cagtagataa agaaactctg
     9361 ctttactata aagaagctct agatggacag attcttgaag aaggtattac agaagcaggc
     9421 tctatgtctt cattcatagc tgcaggtact tcatattcta cccatggaat aaatactatt
     9481 ccctttttca tatattactc aatttttgga atgcagagaa ttggagacct tgtgtgggct
     9541 gcaggggata tgaaatgtaa aggatttttg ataggcggca cagcaggcag gactacactt
     9601 gccggtgaag gcctccagca tcaggatggt catagtcacc tccttgcata tccggttcca
     9661 aatttactgg cttatgaccc tgcatatgca tatgagatag ccgtaattat taaaaacgga
     9721 atggaaagaa tgtatgtaaa aggcgatgat gtattttatt atctaacagt aatgaatgaa
     9781 ggctatatcc aaccgcctat gccaaaagga tcagaggaag ggattattaa gggaatttac
     9841 aaactaaaca gttcagataa taaggatagc aaactaaaag tctcacttct tggtagcggt
     9901 actatattaa atgaaagtat aaaagctaaa aagattctgg aagaagaata tgaattgagt
     9961 gtggatcttt ttagtgtaac aagctataaa gaattatata aagactgttt ggaaacggaa
    10021 cgctggaata tgcttcaccc atctgagatt tcaaaatcaa cttttttaag caaagtaata
    10081 gatcacgatt cagatataat tgtagctgct tcggactatg ttaaaactct tccagagtcg
    10141 atttcttcat ggttaccggc taaatgtata agtcttggta cagatggatt tggaaggagt
    10201 gctagtagag atgacttaag atcatttttt gaagtagatt acagatacat tgtacttgcc
    10261 agtttatatg gtcttagcaa agataaaaaa gtaaaaacag aacttgtaca aaaagcatgc
    10321 aaaaaatttg atatagacac tacaaaagat aatcctttta taagctaatg gaggctatac
    10381 atatatggaa tttaaacttc cagaacttgg tgaagatata gaaggcggag atattattgc
    10441 aattgctgtt aagaagggag agactgtaaa gaaagaacag actctcatgg aaatagagac
    10501 tgataaagct gttattgagc ttccttcacc t
//
LOCUS       DBNGSV010000243        21254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_458786, whole genome shotgun sequence.
ACCESSION   DBNGSV010000243 DBNGSV010000000
VERSION     DBNGSV010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_458786"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..970)
                     /locus_tag="AC159U_08960"
     CDS             complement(35..970)
                     /locus_tag="AC159U_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3337228.1"
                     /translation="MINSINKIILAAFAVIAFMSASHTSVYAKVKVVTTIPDFGSITK
                     EIGGDKVEVKSIVKGYQDPHFVDAKPNYMLWLNKADLLIYNGLELEAGWLPVLITGSR
                     NSKIISSNAPGHLDASSLIQNKLEVPNSKLDKSMGDIHSGGNSHYLLDPRNGIPIAAG
                     IAKRLKAIDPDHSQFYDQNLLAFTSKLKERITAWEEKLEHLKGINLVTYHKSWLYFTG
                     WAGFNEVGYIEPKPGIPPSPSHIAGLVKQMQEKDLQLILSESFYPQKTVRLVADKTDA
                     KLLVLPTMVDRDNGVPTYFDLFDKITEEISKALAK"
     gene            complement(994..2379)
                     /locus_tag="AC159U_08965"
     CDS             complement(994..2379)
                     /locus_tag="AC159U_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337229.1"
                     /translation="MKVVLTAATTAVFSLIFLTGALFADEKESLRNEISELKQMMIKM
                     QQRLDDLEQRNKELEEQSRAKQKTSQSSSEPNAIAEQSPTDGGFIQRAIQTLNPDISV
                     IGIFSASYFSENDPLAGAEADPEDTGVDLQEIEVVFSGSIDPYFRFDSAFSINREGIE
                     TEEAYATTLASLPLNSQYRIGIIRSKFGRINTQHRHSQNFVTLPLVANAFLGEHLNPT
                     SIEANFLVPVPWFFELSAAVGSPEVETPSFDRDEDKNNLGRLLYVFHLKNFFELSESL
                     SVNLGGSFATGANGTFEGSRTNLYGADLFVKYRPLHADPYKELTFQSEFMYRDAESEI
                     GDLNDYGFYSQIIYRFAKRWNAGARFGMTNTDDPLDFEEDEGPEIELLSNDNFFNFTN
                     SGEEEEHEKGGLGLFGQTYRVSAMLTFNPSEFSTIRLQYDYTDPDFTANQHALFLQFQ
                     YSLGAHGAHPF"
     gene            complement(2441..2794)
                     /locus_tag="AC159U_08970"
     CDS             complement(2441..2794)
                     /locus_tag="AC159U_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337230.1"
                     /translation="MRFIDINIRAGNIVNNKKYFTILFSALFLFSLFIHTHPLSLSAD
                     LSFESNNTEQHLNYSHNSEFCSACRADGKIYKTNKKVDLNLHTSILLIKDIGQSYNDL
                     HFKLLNESRAPPVFS"
     gene            complement(2791..3636)
                     /locus_tag="AC159U_08975"
     CDS             complement(2791..3636)
                     /locus_tag="AC159U_08975"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3337231.1"
                     /translation="MSEVVRISQLSFSYNDGVSIHYGGKDFIVNRGEKVAILGPIGSG
                     KSTLLQHILGLLKSDAGTIEVFGHDPAKESAQIRQRIGTLLQNVDQQIIAPTVWDDIC
                     FSPRNYGMDSSETEKRVNYLMEWLDIKHLKNKVPHYLSGGERTKVGIAGALAHKPELL
                     ILDEPFEGLDPVCRQDLISLLNEINENEKTSIILSTHNINVVPLIAEIVYIIAEGGEI
                     IAKGSPEEIFTKVDMLRSCHIEPPVLSALFHELKNNGIDLDHSFNIKEAASIIAKSKL
                     NLKEK"
     gene            complement(3633..4445)
                     /locus_tag="AC159U_08980"
     CDS             complement(3633..4445)
                     /locus_tag="AC159U_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR3337232.1"
                     /translation="MDIGAIDYYANFGNSLIHRLGPGIKVIFVISLIASIVLTKSLII
                     LGGIYFLLLGLVIISKVPAIKVILLAAYPAIFAIIFAFASWSGNWAFTLLIIMKALSA
                     SLCMVLLIITTSYPRVFSAMRPIIPGILFDALLLTYRSIFLLFGLLSNLFSALKIRGG
                     FSNKNYFKRLKNLSSGLGLLVIRGFDLSQHYYGVLNIRGYSGNFSVSGGEDLEKLSDY
                     LFLVLGFATLVLTVYCEEFDNKITGAYFLISSALFLGIILLRSLVLKLKGDR"
     gene            complement(4450..5178)
                     /locus_tag="AC159U_08985"
     CDS             complement(4450..5178)
                     /locus_tag="AC159U_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR3337233.1"
                     /translation="MSHIHIPDGILPMWLLLIGWLLTSFFLFLSIKRVKKEEVSLKLP
                     YIGIVSAIMIVAMMLEIVPIAYHFNLSVIAGIILGPAMAFISVFIVDLIIAMFGHGGI
                     TVIGLNTLVVGTEAVAGYYLFHFLLGVLKNISNSHAVSASLATVCSLVLSCALMIGFV
                     CASNLDSSAEKIEKINKQNVFLEETGLAGHKDHEHSQTIDAYRFTKMVVPLSIIGWIL
                     EAVITGFVIKYIHRIRSPLILRGT"
     gene            complement(5182..5622)
                     /locus_tag="AC159U_08990"
     CDS             complement(5182..5622)
                     /locus_tag="AC159U_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3337234.1"
                     /translation="MDLIVDNYVQSVFKSKGLKCTPQRIAVFKVIEESKTHMSIEKIH
                     AEVKKLLSNVGLATIYRTLDSLIDLGLIEKVHLEDGCHNFTSALSGHRHALVCKVCDQ
                     IVEYEDCPLEGIKEDVSKKTGFEIDNHYLQLFGTCSECQASNNT"
     gene            complement(5823..6584)
                     /locus_tag="AC159U_08995"
     CDS             complement(5823..6584)
                     /locus_tag="AC159U_08995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3337235.1"
                     /translation="MKLENKVALITGGSLGLGKYTAELFVQEGAKVVITGRTEKTLKE
                     AQEDAKKKGIELDYLVSDVSKEDDCKEAVDYTVKKYGKIDILFNNAGVLELGRTHETS
                     TETWDKIFDINVKGIYLMTKYAVPHMLEKGYGCIVNNSSVLGLKALPGVAAYDASKGA
                     VTQLTRSMALDYATDGIRVNDICPGTIVTPMVTNMLDSMEDRDAGEELFKTFHPMGRL
                     GRPEEIAHAVLFLCDDNIGFMTGNMLSVDGGWVAR"
     gene            6679..7452
                     /locus_tag="AC159U_09000"
     CDS             6679..7452
                     /locus_tag="AC159U_09000"
                     /inference="COORDINATES: protein motif:HMM:NF053169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8083 family protein"
                     /protein_id="HIR3337236.1"
                     /translation="MANSVLVVYEPLDELIEKKDYWKKYIDFYEKYWDGPSSFQSVWF
                     GKEFDDFRDALSDHMGMDKEFIEDCFFIKDKDDNYYISPLSENANPYVFSSENYIPPE
                     WFMLFSKEEKKLTYTHTGYGAISQDGIYYDTDISKAAKAIYKAELIIKNGAERSGQSG
                     SSISNILGQLREGVENIVAWLSNFNQKGKIVLSYGDICTYIPNYSLSDENSVQDIWDI
                     LSLIETGNYDEAESKLNIFIIKWQETFNQASSENTSTIQ"
     gene            complement(7570..8202)
                     /locus_tag="AC159U_09005"
     CDS             complement(7570..8202)
                     /locus_tag="AC159U_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337237.1"
                     /translation="MEKTNNRKELRKKLVRLSLVFVAGYVLSLVLWLFVGKYYGYVIT
                     SISAHAIAPVKNIQLDLVEFDKKNKDSISTYFRTLKQFTKNIGMRQDVNFGHFTFNIP
                     ITIGILAAFFPFIRKKRVYVEVAVLLIAMHIIYVFFLEGLKLSEALLKSGYEKPNEAK
                     LVFWQFSEGFMKSMVLRFEPFLIGAYLYFSRTRGLGKPAETDSGDEIEEN"
     gene            complement(8171..8740)
                     /locus_tag="AC159U_09010"
     CDS             complement(8171..8740)
                     /locus_tag="AC159U_09010"
                     /inference="COORDINATES: protein motif:HMM:NF021254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HIR3337238.1"
                     /translation="MGKKSKKHQIKEILDKNKSTFSILKFIVTYVVLMGGFFALLRFE
                     IVGGFGDLYSVYESGLIASCVKILNFLGLKTSGQGSLLFLPGITFDVRFGCNGLEAVL
                     IYAVAVISFPAKWLKKLIGIAAGLVIIQIINLLRIVGLAFSGLYYREIFDLLHIYIAQ
                     GIMIAVALGVFLVYIYYLGHGEDKQQEGA"
     gene            complement(8901..9620)
                     /locus_tag="AC159U_09015"
     CDS             complement(8901..9620)
                     /locus_tag="AC159U_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3337239.1"
                     /translation="MNNYKQLTIASYIFRTTKFILPIILGALVLGFVFSAPLRDMHTY
                     RQTGNLLGNIVDNLNLTGLDDSSYNLSYKVASDQILKNIRRYSQNLEKEEAQMLADLI
                     NKECKRYNLNPYLVLAIIKVESSFNPMAVSSKGAIGLMQVMPETAFYLASKKGMIIDD
                     ESLIADPLINVQLGIYYFHNLLKRYNKLESALFAYNYGPGRFETITNSEENEVKMPEY
                     VRKVITFKKYLERKSLIAKQS"
     gene            10039..10905
                     /gene="ispE"
                     /locus_tag="AC159U_09020"
     CDS             10039..10905
                     /gene="ispE"
                     /locus_tag="AC159U_09020"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3337240.1"
                     /translation="MDSITLLCPAKLNLTLEILGIRTDGYHVLRSIMQPVDLFDELTV
                     CSESGSGIELETEGLKLPDIKENLVYKSAELYLKETGISTRLQLKLKKKIPIQAGLGG
                     GSSNAAATLVGLNRIYYALSTDRLIELAAQIGADVPFFIHCRTALMEGIGEKITVLND
                     FPLLNYLIVKPSFGLSTADVYKKWDEIGTSSNNNLNIGEIIEKYQNGQVILRNDLEQA
                     AESLNSGISKLKELLKQLGCSNVLVSGSGTAVFAIFTSQNEAKDIYDYLNDSGDYSVF
                     LAKGISGWHRLS"
     gene            10950..11023
                     /locus_tag="AC159U_09025"
     tRNA            10950..11023
                     /locus_tag="AC159U_09025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10982..10984,aa:Gln,seq:ttg)
     gene            11177..13057
                     /locus_tag="AC159U_09030"
     CDS             11177..13057
                     /locus_tag="AC159U_09030"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HIR3337241.1"
                     /translation="MSLKIRDDLKESYPDVYTPEALSALEALSKFNSEQKAIMEKRYK
                     KRADRAKNKERIDFLDPNSVIPRTNIKVQDARDGKFEGGEIPADLQRQWIQGTGPAAK
                     PDSPTEVSIRNVAYAILSGADGWMFDGEDALGQITSMSLDNQRNLKLAIDKDPVFLKT
                     SEKVAASMNKWGNQFFGRDIIEDWEKQLDFTTKIFRARGLHLDDRHIRDENGVAMSAS
                     IVDMVLYVANNYKTLQANGSSICLYLPKIQTAEEAALWNEMLSALENHIGIAEGTIKV
                     YVLIEQLEETYQLMEIRAALGKHFVGFNTGRWDYINSVSDAMAWDKDFINPNIESITM
                     TYSYMAAYEDRVRRAVNTPDINGNFALWQGGMEPNIPVGTEEGVASSMEKAVAGAERE
                     QREGASGKWVAHWKMVHIVRPVWEKVGEANQLGRKFPPLTYTEEDSQALTHLEPAPRS
                     IRGARNLLSVALQYGNAFGQGFQAAALKPADFFEDDNILYLMEDAATGEIRLSILWEW
                     IHKGAKLNEDDAETGLKAGDTFTVEIFNKLLEEEYEKLRNAKDKDVYDISKDTTLPIA
                     KEIVRTYVLDEVKVPWYIDLLNINLNNHDLQTAKKRIQQYMTAFKKDGTRITENLDFV
                     VS"
     gene            13094..15400
                     /gene="aceA"
                     /locus_tag="AC159U_09035"
     CDS             13094..15400
                     /gene="aceA"
                     /locus_tag="AC159U_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HIR3337242.1"
                     /translation="MGSMESEAKRIKSYFSSQRFKGITRLYSAEQVVAQQGTIKNDYT
                     IAKEASIKFYKRLRELYSKKKSITTYGPYSPGQVIMMKRAGIEGIYLGGWATSAKGSI
                     TEDRGADLASYPLSQVPDDAAVLVRALLSADKNQQFERSRMTEKARKATPEIDYTPYI
                     IADADTGHGGDAQVRNLIRRFVEVGVTGYHIEDQKPGTKKCGHQGGKVLVPVDEQIKR
                     LNAARFQLDIMEVPGIIVARTDAEAATLLEGRGDERDHPFLLGVTNLTVPAYKDAYLS
                     ILKRFYDKGIKDINGHLLYKISNDVYKSCYAWLKDVGVLEIADSNIEAYKKGKKTSLD
                     KTLDDVVNKFVETWEAESGIKTFRKAVADRMEFRAEEGTDFDMTAEQWDEFAKQVSLS
                     EAQKKAKDMGLNVTWDCELSRTPEGYYQIQGGIEYAIAKSLAVAPFADILWMETKTAD
                     LADAKEFADAIHAVFPDQMLAYNLSPSFNWDTTGMSEQEMKDFPSEIGKLGFVFNFIT
                     YGGHQIDGLASEEFSRALLEDGALALARLQRKLRLLDSPYRTPQSYVGGPRMDAMLTA
                     SSGRTATTKAMGKGSTQFQHLVQTEVPTKVLEDWLEIWAKHYKIKGSLRVELRPNRAG
                     SDLLELNILSSRSKNKMADVIFGSIQDLRGKNIISIRDQNTYSTEFRQKRLMTIIHLF
                     LIHRYKGDSVHYVNPTDDNMKQTQGMKKLGIYSEVNTEVGDIIVAGINAKKVKDLLSE
                     DQVDLKNLIAKKDSAKKPAAKKTASKRK"
     gene            complement(15495..16124)
                     /locus_tag="AC159U_09040"
     CDS             complement(15495..16124)
                     /locus_tag="AC159U_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3337243.1"
                     /translation="MNFISKLLLLIIVLAAGLAFALYKGMYNVGATSPHNKAVKLILH
                     ESMEMSVKNHAKDIKAPDLGDEKMINSGFVQYDSMCAMCHGAPGIPESVLHIGLNPEP
                     PELYEENDEWTQSELFWITKNGIKMTGMPAYGPTHSDEEIWAIVAFLQVLPNLSEQEY
                     KSYALKNKNVDDGHDHVHGSEIEKNMKTLLEDKKDSDVHIHEDGSEHLH"
     gene            16399..16767
                     /locus_tag="AC159U_09045"
     CDS             16399..16767
                     /locus_tag="AC159U_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3337244.1"
                     /translation="MSKKILLVCDDNEKSEVLSSALLSSGYETNSLSDYSDIVNKSVE
                     VNPDMIIMDIDIPIKDGYKICRELKEAPDTQHIKVIFVKNDNNPLEKAWSVVKGADFY
                     FATKNPFSENNLIQSMSVLN"
     gene            complement(16780..17910)
                     /locus_tag="AC159U_09050"
     CDS             complement(16780..17910)
                     /locus_tag="AC159U_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3337245.1"
                     /translation="MKKKVLGGREQVKVAVVQASPVFMDKDKSIKKACKLIKEAGKNG
                     AELIVFSEAFIPGYPAYYSVGYETAPHEWTDYMIAIQENSVLIPGEDTEILGKAAKSA
                     GAYVVIGCNEIDDRAGSCTVFNSLLFIDKYGQVMGRHRKLMPTYTERVYWGMGDGSDL
                     DVYDTEIGRIGGLICWENHMPLARVSMINQGEEFHVCVWPGNWKRGADQLLDADTGEG
                     GGGCNLQNLIKVHAFEAGAFVLSCCGYMDEKDFPNRWEYIRDSDHMNFSWARGGSSIV
                     NPAGRYLVEPNFEKDAILYTDCYANQIKATKAIFDSLGHYARWDVLQLQRNRTNRLEP
                     EVIGSKSISELQISNEDIDRISSEYEIDKEKVQRLIQELNLT"
     gene            complement(17938..18354)
                     /locus_tag="AC159U_09055"
     CDS             complement(17938..18354)
                     /locus_tag="AC159U_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337246.1"
                     /translation="MEENNQNQQDQKAPGMMDSVLMAFNMGVKKMQEEGQIKNVLEAG
                     DELFQEIYKNLQDAYGTAIDETFLKANAEFFLNIALMGYIFPSICAYDDNFKDNLFEL
                     IEKKLEQSLKRRQQGGGPGDPGGAPGGAPGGSNIIV"
     gene            18474..20162
                     /locus_tag="AC159U_09060"
     CDS             18474..20162
                     /locus_tag="AC159U_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3337247.1"
                     /translation="MPKVTGGWLVAKTLSDLGVKHIFTLSGGHINPIYNACRQLGITL
                     IDTHNEQGASMAADAYGRITRKPAVCLTTAGPGLTNALTGIAGSYLANSPCLYISGRS
                     GVEENDKLPLQEIDQKSMVTPVTKWARDIYDIKRIPEYLSIAYNKAVSGRAGPVYLGM
                     SYEVLYPEIKKSDAKSMYSPAPSNKIEPDADTIKEALELLIKAKKPVVLAGSGTWYSD
                     CAEQLEQFIKLTGIPVFTLNLGRGIISDENEYCMGSASPSAPNGFRDITGKADLILML
                     GIRLSLYIGFGKTFNPKAKIIQVDIQADEIGRNRAADLSIVADAGKVLEKCIDEADQQ
                     GVQFNYKKWLKNAKNIRNDAWKETARIRKSNKKPIHALRVIKAVENVMREDGMIVIDG
                     GDTQAFSDGTYNVRQPGHYIKGGPLGCMGTGIPFALGIKAACPDKQVGLITGDGAFGM
                     NFMEFETAVRHNLPFVAVVCNDEGWGMTKHQMWISFGKKTPTVGVDLPFTPFHELANV
                     LGGYGEMVTDPINLEKAIKRGLDSGVPAIINVKTDPEAISRATYGLTEMMTPKD"
     gene            20171..21208
                     /locus_tag="AC159U_09065"
     CDS             20171..21208
                     /locus_tag="AC159U_09065"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HIR3337248.1"
                     /translation="MKLRLPAEWEKHEATWLAWPKRREDWPGKYSSIKWVYADIIKAI
                     SYSETLNLIVDSEQAEQKVRAILKKSSVDLNRIKFYHINTDRSWIRDYGPFFVRLQNK
                     INALSFKFNGWAKYSQWKNDNKVASHITSNLNIESIRAEYYNKEIILEGGSIDVNGKG
                     TLITTRQCLLSSSKQVRNRGLSKEDYEQIFNNYFGITNTIWLNEGIAGDDTNGHIDDI
                     CRFVNTDTVVACHESNPDDTNYKYLKENLEILGESRLEDGSKINVIKLPMPEPKYFDG
                     YRLPASYANFYITNKCVLVPTYNDTNDYKALGILGELFSDRKVIGINCVDLIWGLGAI
                     HCMTKEQFAQS"
BASE COUNT         6749 a         4232 c         4084 g         6189 t
ORIGIN      
        1 taaccaatct caactttatc tagtgtaatc atatttactt tgcaagagcc tttgaaattt
       61 cttcagtaat tttatcaaac aaatcaaaat atgtaggcac accattatca cgatctacca
      121 ttgtagggag cacaagcagc tttgcatctg ttttgtctgc cacaagtctt acagttttct
      181 ggggataaaa tgattcagac agtattagct gtaagtcttt ttcctgcatt tgcttaacta
      241 aacctgctat atgtgatggc gagggtggaa taccgggttt tggttctata taacccactt
      301 cgttaaatcc cgcccagcct gtaaaataca accagctttt gtggtatgta accagattaa
      361 tgccttttaa atgctcaagc ttttcttccc acgctgttat ccgctctttt agtttagaag
      421 taaaagcaag cagattttgg tcataaaact gagaatgatc gggatcaatt gcctttaatc
      481 tttttgctat gcctgctgca atcggtatcc catttctggg atcaagcaga tagtgagagt
      541 taccacctga atggatgtct cccatgctct tatctaattt agaattcggt acttccagtt
      601 tattttgaat tagagaagat gcatctaaat gacccggagc atttgatgaa atgatctttg
      661 aattacggga accagttata agtactggca gccagccagc ttcaagctca agaccgttgt
      721 atatcagcaa atcagcttta tttagccaaa gcatatagtt tggcttcgcg tctacaaagt
      781 gagggtcttg ataacccttt actatacttt tgacttcaac cttatcaccg ccgatctctt
      841 tggttatcga gccaaaatca gggatagtcg taacaacttt tacttttgca taaaccgatg
      901 tatggcttgc tgacataaaa gcaataacag caaaagctgc taatataatt ttattaattg
      961 aatttatcac tgttattttc cttattaaca tgatcaaaac ggatgcgctc cgtgcgctcc
     1021 gagtgagtac tgaaactgca gaaacagcgc atgctgatta gcagtaaagt cagggtctgt
     1081 ataatcatac tgaagcctta ttgtcgaaaa ctcagaagga ttaaatgtta gcatggcaga
     1141 cactctgtat gtttgtccaa aaagtccaag tccacccttt tcatgttctt cctcctctcc
     1201 tgaatttgta aaattaaaga aattgtcatt ggacaacaat tcgatttcag gaccttcatc
     1261 ttcttcaaaa tccagtggat catccgtatt agtcatacca aaccttgctc ctgcattcca
     1321 tctctttgca aatctataaa taatctgaga ataaaaacca taatcattta gatcgccaat
     1381 ctcactctca gcatctctgt acataaattc ggactggaat gtaagctctt tataagggtc
     1441 agcatgtagc ggcctgtact taacaaaaag gtctgctcca taaagatttg ttcggctccc
     1501 ctcaaatgtt ccatttgcac ctgttgcaaa tgagccaccc aagtttactg aaagactttc
     1561 tgatagctca aagaaatttt tgagatggaa tacatatagc agtcttccca ggttgttttt
     1621 gtcttcatct ctgtcaaaac tgggagtttc aacctccgga ctacctacag ctgcactaag
     1681 ctcaaaaaac catggtacag ggacaagaaa attagcctca atagaggtag gatttaaatg
     1741 ctctcctaaa aaagcatttg ccacaagtgg cagggtaaca aagttttgag aatgtctatg
     1801 ttgagtatta atacgcccaa acttgcttct tataattcca attctatatt gagaattaag
     1861 aggcagtgaa gcaagcgtgg tagcataggc ctcttcggtt tcgatgcctt ctctgttaat
     1921 tgaaaacgcg ctgtcaaatc tgaaataggg atcaattgat ccggaaaata caacctcaat
     1981 ttcctgcaga tcaacacctg tgtcttcggg gtctgcctca gcaccagcaa gcggatcatt
     2041 ttccgaaaaa tatgatgctg aaaatattcc tattacgctt atatccggat ttaaggtctg
     2101 tattgctctt tgtataaaac caccatctgt aggcgattgt tccgctattg cattgggctc
     2161 gctgctgctt tggctggttt tctgtttggc tctcgactgc tcctctagct ctttgttcct
     2221 ttgctccagg tcatcaagcc tctgctgcat tttaatcatc atctgcttaa gctcgctgat
     2281 ctcatttctt aaactctcct tttcatctgc aaacaaagcg cctgttaaaa atattaatga
     2341 aaataccgct gtcgtagcgg cagttagtac tactttcata attcacctcc acaaattcta
     2401 ctacttataa attctggaat tcaaaaaact gaaatgggaa ttatgaaaat acgggagggg
     2461 ctcttgattc atttaagagc ttaaaatgta aatcattgta agattgccct atatctttga
     2521 tgagcaatat cgaagtatga aggtttaagt ctaccttttt gttggttttg tagatttttc
     2581 cgtcagccct gcaagcagag cagaattcgg agttgtggct gtagtttaaa tgctgctcgg
     2641 tgttgttact ttcaaaactt aagtctgcag ataaagaaag aggatgtgta tgtatgaaaa
     2701 gtgaaaagag gaaaagtgcc gaaaaaagta tagtgaaata ctttttgtta ttaactatat
     2761 tcccggctct aatgttaata tcaataaatc tcatttctct tttaagttta acttactctt
     2821 tgcaataatt gaagccgctt ctttaatatt gaaactgtgg tctaaatcaa taccattatt
     2881 tttaagttca tggaacagag cagacagcac agggggctca atatggcagc tacgcagcat
     2941 atcaactttt gtgaaaattt cctcaggact tccctttgca ataatctctc cgccttcagc
     3001 aattatatag acaatctccg ctatcaacgg aactacgttt atattatgag tcgagagtat
     3061 gattgatgtt ttttcgtttt catttatttc atttagcagt gatatgagat cttgtctgca
     3121 aacaggatca agtccttcaa aaggctcatc caaaattaat agttcaggtt tatgagcaag
     3181 cgctcctgca attcccacct ttgttctctc tccaccgctt aagtaatgtg ggactttgtt
     3241 tttaaggtgt tttatatcga gccattccat tagatagttc actctttttt cagtttcact
     3301 actatccatc ccgtaattcc ttggtgaaaa gcaaatatcg tcccacacag tgggggcaat
     3361 aatctgctga tccacatttt gaagaagcgt gccaatgcgc tgccttatct gggctgattc
     3421 cttagcagga tcatggccaa acacttctat tgttccggca tctgatttca gaagacccag
     3481 aatgtgctga agcagggtgg acttaccact tcctatcgga cccagaatgg cgactttctc
     3541 accacggtta actataaagt ccttaccacc gtagtgtatg cttactccgt cattatacga
     3601 aaagctcaat tgacttattc ttactacttc gctcatctgt ccccctttaa tttcaacact
     3661 agtgatctga gcaaaataat cccaagaaat aatgcagacg agatgagaaa atatgcacct
     3721 gtaatcttat tatcaaattc ctcgcaatac actgttagca caagggttgc aaagcctaaa
     3781 actaaaaaca gatagtccga caatttttct aaatcctcac ctccggagac cgagaaattg
     3841 ccagagtaac ccctgatatt aagcacgccg taataatgtt gagagaggtc aaaacctctt
     3901 attaccaaaa gccccagtcc tgatgacagg ttcttaagtc tcttgaaata atttttgttc
     3961 gaaaaccccc ctcttatttt aagtgcgctg aagaggtttg atagaaggcc aaagagtaga
     4021 aatattgacc tgtatgtaag taaaagtgca tcgaataaaa taccgggaat tatcggtctc
     4081 attgcgctga acacccttgg ataagaagta gtaataatta acagcaccat acaaagggat
     4141 gccgacagtg ccttcataat aataagcagc gtaaaggccc agttgccgct ccagcttgca
     4201 aatgcaaata ttatggcaaa tatagcaggg taagctgcaa gaaggattac ttttatagca
     4261 ggcacctttg agattattac aagccccagc agtaaaaaat atatgccccc caggataata
     4321 agactctttg ttagaacaat gcttgcaata agcgatatta caaagattac ttttatcccc
     4381 gggccaagcc tgtgaataag gctatttcca aaatttgcat agtagtctat tgctccaata
     4441 tccatatagt tatgtacccc gcaaaataag gggtgacctt atcctgtgaa tatattttat
     4501 tacaaacccc gtaataacag cttcgagtat ccagccaata atactgagag gaaccaccat
     4561 ctttgtaaac ctgtatgcat caatagtctg agagtgttcg tggtctttat gtccagctaa
     4621 tccagtctct tctaaaaaca cgttttgttt gtttatcttt tcaatttttt cagcagacga
     4681 atccaggttg gaagcacaca caaaacctat catcagagca cagctgagaa caagactgca
     4741 cacagttgca agcgaggcag aaactgcgtg ggagtttgag atatttttta aaacaccaag
     4801 cagaaaatga aacagatagt atcctgccac agcttctgtt ccgacaacaa gggtgttaag
     4861 accaattact gtaattcccc cgtggccaaa cattgctatt atgaggtcta caataaaaac
     4921 tgaaataaat gccatcgccg ggccgaggat tattccggct atcacgctca gattaaagtg
     4981 ataagcgatt ggaacaatct ctaacatcat agccacaatc attattgcag aaacaatccc
     5041 aatatatgga agcttaagcg acacttcttc tttcttaacc ctctttatag acaaaaagag
     5101 aaagaagctt gtaagaagcc agcctataag caatagccac atgggcaaaa ttccgtctgg
     5161 tatgtgtatg tgtgacattt attatgtatt gtttgatgcc tgacactcgc tgcaggttcc
     5221 aaaaagctgc aggtagtggt tatctatctc aaaccctgtt ttttttgata catcttcttt
     5281 tataccctca agcggacagt cctcgtattc tacgatctga tcacacacct tgcatacaag
     5341 agcatgcctg tgaccggaaa gagctgaggt aaaattatga catccgtctt caaggtgtac
     5401 tttttctatt aaccccagat caatcagtga atcgagggta cgatagatcg ttgcaagccc
     5461 cacatttgag agcagctttt taacctcagc atgtattttc tcgatagaca tgtgagtctt
     5521 agactcctct ataactttaa atacagctat cctctgagga gtacacttaa gcccctttga
     5581 cttaaaaaca gactgcacat agttatcaac aatcagatcc attgtaataa ttgagacatt
     5641 ttattaattg agaaagattc tcaattatag aaatgtttag caagatgtca ataaataata
     5701 tgtatatgta tctgcgttac agatataaga atttatagaa tatattttaa gcacccctgc
     5761 ccattagtgt agggcagggg ctttattatt gaagttaaaa attggttttg taaataaagt
     5821 agttatcttg cgacccaacc gccgtctaca gacaacatgt tccctgtcat aaaccctatg
     5881 ttgtcatcgc agaggaaaag cacggcatga gcaatttctt caggtcttcc cagtcttccc
     5941 ataggatgga atgttttaaa cagctcttct cctgcgtctc tgtcttccat ggaatcgagc
     6001 atgtttgtta ccataggtgt tacgattgta ccaggacata tatcatttac ccttatgccg
     6061 tctgtagcat aatctagagc catactgcgg gtaagctggg taacagcacc ttttgaggcg
     6121 tcatacgcag ccactccggg aagcgcttta agaccaagta ctgaagagtt gttcactata
     6181 catccatacc ccttctcaag catatggggt acagcatatt tggtcatcag atagattccc
     6241 tttacattaa tatcaaaaat cttgtcccac gtttctgtgg atgtctcatg ggtccttcca
     6301 agctcaagca cccctgcgtt attaaaaagt atgtcgatct taccgtactt tttcaccgta
     6361 taatcgacgg cttccttgca atcatcctct ttcgatacat cgcttacaag ataatcaagc
     6421 tcaatgccct ttttctttgc atcttcctga gcctctttta gtgttttttc tgttcttccc
     6481 gttatcacaa cctttgcgcc ttcctgcaca aaaagctcag ctgtgtattt accaagccca
     6541 aggcttccgc ctgttataag ggctactttg ttttcaagtt tcattctaat atatccttaa
     6601 tgtaatttta gattacaaat tataacctaa tcttatcata atttgttaat ctttttttaa
     6661 gggctaaaat taaaagttat ggcaaattca gttttggttg tttatgaacc cttagatgaa
     6721 ctaattgaaa agaaagacta ctggaaaaag tatatagatt tttatgaaaa atactgggac
     6781 gggccatctt catttcagtc cgtgtggttt ggaaaggagt ttgatgattt cagagatgcg
     6841 ctttcagacc atatgggaat ggataaggag tttatagagg actgtttttt tattaaggat
     6901 aaggatgaca attattatat atctcccctt tcagaaaatg ctaaccccta tgtcttcagc
     6961 tctgaaaatt acataccccc tgagtggttt atgctcttta gcaaagagga gaaaaaactt
     7021 acttacactc acaccggtta cggggcaata tcgcaggacg gcatctatta tgatacggat
     7081 atttcaaagg cggcaaaagc catatataaa gctgaattaa ttattaaaaa tggcgccgag
     7141 agatctggtc agtcaggttc gtccatttcc aatattctgg gtcagctaag agaaggggtg
     7201 gaaaacattg ttgcgtggct atctaatttt aaccagaaag gaaagatagt cctcagttac
     7261 ggggatatct gcacatacat tccaaactac tctctttctg atgaaaactc tgtacaggat
     7321 atttgggata tattaagtct gattgaaacg gggaattatg atgaagcaga atcaaagctg
     7381 aatattttta taatcaaatg gcaagagacc tttaatcaag cttcttctga aaacacctct
     7441 actattcagt agcaccttaa aaaggcttta gcagtttcga tctaatttgt agatttgctg
     7501 taaatctctt cactgcatct tattactttt ttgtacatga aacagttaga aagactacag
     7561 tcccgattct cagttctctt caatttcatc accagagtca gtttcggcag gctttccaag
     7621 ccccctggtt ctggaaaaat acaggtaggc gccgatgaga aacggctcga acctcagcac
     7681 catactcttc ataaaccctt cagaaaactg ccagaacact agttttgcct cattaggctt
     7741 ttcatatccc gactttaaaa gcgcttcaga caactttagc ccctctaaga agaacacata
     7801 aataatgtgc atagctataa gcagcactgc tacttcaaca tacacccttt tttttctaat
     7861 aaagggaaaa aacgctgcca gtattccaat ggtaatcggg atgttaaaag taaaatggcc
     7921 gaagtttaca tcctggcgca ttccaatatt tttggtgaac tgttttagag ttctaaaata
     7981 tgtactgata ctgtctttat tttttttatc aaattctact aaatcaagct ggatgttttt
     8041 tactggagca atagcatggg cagagattga ggttataaca taaccatagt actttcccac
     8101 aaaaagccag agcactagag aaagcacata gccggcaaca aatacaagtg agagccgtac
     8161 tagttttttt ctaagctcct tcctgttgtt tgtcttctcc atgtcccaag tagtaaatgt
     8221 aaaccagaaa taccccaaga gccacggcaa tcatgatccc ctgggcaata tagatgtgaa
     8281 gtaaatcaaa tatttctctg taatataaac ctgaaaaggc aagacctaca attcgaagca
     8341 agtttataat ctgaataatt accagccctg cggctatacc aataagtttc ttgagccatt
     8401 ttgcaggaaa acttattact gctaccgcat aaatcagtac agcttcaaga ccattacagc
     8461 caaacctcac atcaaaagta ataccaggca gaaataacag tgagccctgc cccgaggtct
     8521 ttagtcccag aaaatttaat atttttacac agcttgctat aagaccggat tcatagacgc
     8581 tgtatagatc accaaatccg cctactatct caaatcttaa aagcgcaaag aatcctccca
     8641 ttagtactac atatgtaact ataaatttca gtatactaaa agtactttta tttttatcta
     8701 atatctcttt tatctggtgc ttttttgact ttttacccat gggtcaggtt cgttgtaaaa
     8761 ctgctctgta aatatgatta ctttttcaga tcaggataaa tatacctgaa attttacaag
     8821 cgtaatgagt aattaaagaa atactgaaaa aatgattttc taaaacttcc taaatcatct
     8881 aaaaaaaggg atgtattata ctaactctgc ttcgcaatca ggctctttct ttcaagatat
     8941 tttttaaaag taatcacctt tctcacatac tccggcattt tcacttcatt ctcttcactg
     9001 ttagtaattg tctcaaatct cccggggccg tagttataag caaatagagc gctttccagc
     9061 ttgttatacc gtttaagcag attgtgaaag tagtatatcc cgagctgtac attaatgaga
     9121 gggtcggcta ttaagctttc atcatcaatt atcatacctt ttttggatgc aagataaaaa
     9181 gctgtttcag gcatcacctg cataagacca attgcaccct ttgaagatac tgccatggga
     9241 ttaaagctgc tttctacttt aattatggca agcacaaggt agggattgag attatagcgc
     9301 ttacactctt tatttataag gtctgcaagc atctgtgctt cttctttctc aagattttga
     9361 gaatacctgc gaatattttt tagaatctgg tcactggcaa ccttgtagct aagattatat
     9421 gagctgtcgt caagccccgt taggttcagg ttgtctacaa tgtttccaag caaattcccg
     9481 gtttgcctgt atgtgtgcat gtcccttaaa ggcgctgaaa atacaaatcc caaaacaagt
     9541 gcacctaaga ttatgggtag tatgaatttt gtagtacgga aaatatagct ggctattgtt
     9601 agctgtttgt agttattcat gaatcttgtt tcatacctcc aaaatgcata taacttttag
     9661 ttaatgatta cagaaagctt aaaaaaattt agactttttt gtgatttaat tacctgagat
     9721 ttagttaatt gcttatttag atataacttt ttaaaaatgt tttaaactta aacaaaatct
     9781 aacactaatt taatattaag ctttacttat catcaacctc tttatttatg tttaagatat
     9841 cctgtatttg ccaaatatta gtgatatctg tttttcgctc cctttcttgc tccctcttaa
     9901 tactttaata ttaatttacc cgcttatttt atttcttcaa ctacaaattt tatgatttta
     9961 gtcttagctg tataatccga gaaccacata ttttttgact gatctattat taccttaaag
    10021 tttatataaa ataaagtcgt ggattcaatc actttactat gccctgcaaa gttaaacctc
    10081 actctggaaa tattaggcat aagaaccgat gggtatcatg tacttagatc aatcatgcag
    10141 ccggttgatc tgtttgatga gcttaccgtt tgttctgaga gtggatcagg tattgaactt
    10201 gaaactgaag gattaaaact tcctgatatt aaagaaaacc tggtatacaa atcagctgag
    10261 ctttatctaa aggaaacagg tatatctaca aggcttcagc taaaactcaa aaagaaaatc
    10321 ccgattcagg caggtcttgg cggagggagc agcaacgccg cagccacact tgtaggactt
    10381 aacagaatat actatgcact aagcaccgac aggctgatag aattagccgc tcagattggg
    10441 gcggatgttc cattttttat tcactgccgt acggcactca tggaaggtat tggggaaaaa
    10501 ataacagtct taaacgattt tccgcttctg aattacctca ttgttaaacc atcttttgga
    10561 ctttctacag cggatgttta caagaaatgg gatgaaattg gcactagcag taacaataat
    10621 ttaaatattg gagaaataat agaaaaatat caaaatggac aagttattct cagaaatgat
    10681 cttgaacaag ctgctgaatc actaaattct ggtatatcca agctcaaaga gctgttaaaa
    10741 cagctgggct gcagcaatgt gctagtgagt ggaagcggaa ctgccgtttt tgcaattttt
    10801 acctctcaaa acgaggcaaa ggacatatat gactacctta atgacagcgg tgattacagt
    10861 gtatttcttg ctaaaggcat atcaggatgg cacagactgt cttaagtact tgatatagaa
    10921 taaaaacctt ggtactatac gggctttttt ggggcgtcgt tcaacggcag gacacaggcc
    10981 tttgacgcct ggtatggtgg ttcgaatcca cccgccccag ccaagaagct ttcttttttg
    11041 gctgaataaa aatgaatttg tattaaaaat gttaaaaatg atgagtaaaa acagattaaa
    11101 agcaactcaa ggtatagcat ttaatttttc aatcaaacta taaatataac cccaaggaga
    11161 gaagaaggag aaaataatga gtttaaaaat acgtgatgat ctaaaggaat cctaccccga
    11221 tgtttatact ccggaagcct taagcgccct ggaagctctt tcgaaattca atagcgaaca
    11281 aaaagctatt atggaaaaaa gatataaaaa aagggcagat agagctaaga ataaagaaag
    11341 aatcgatttt ttagacccaa attcagtcat accacgtact aatattaagg tacaggacgc
    11401 aagagacggc aagtttgaag gcggggagat cccggcagac ctacagcgcc agtggattca
    11461 gggtacgggc cctgcagcaa aacccgactc tcccaccgag gttagtataa gaaacgtagc
    11521 atacgcaatt ctttcaggtg cagacggatg gatgtttgac ggtgaggacg cccttgggca
    11581 gatcacttct atgtcactgg acaatcagag aaacctcaag cttgcaatag ataaagaccc
    11641 cgtattttta aaaacttcag aaaaagtagc agcttctatg aacaaatggg gcaatcagtt
    11701 ttttggccgt gatattattg aagattggga gaagcagctc gattttacca ccaagatttt
    11761 cagagcaaga ggtcttcacc ttgacgacag acacattcgt gatgaaaacg gcgtggcaat
    11821 gtcggcttca attgtggata tggtcttata tgtagcaaac aactataaaa cactgcaagc
    11881 aaacggctct tccatctgcc tctatcttcc taaaattcag acagcagagg aagcagcgct
    11941 ttggaacgag atgctaagcg cacttgaaaa tcatattggt atcgcagaag gaacaataaa
    12001 ggtatacgta ctcatagaac agctggaaga aacatatcag ctaatggaaa tacgtgcagc
    12061 tcttggaaaa cactttgtgg gttttaatac aggcagatgg gactatatta acagcgtatc
    12121 tgatgccatg gcatgggata aggattttat caaccctaat attgaatcaa ttacaatgac
    12181 gtacagctat atggcagcct acgaagacag agtaagaaga gctgttaata ctccggacat
    12241 taacggcaac tttgctctat ggcagggcgg tatggagcct aatatcccgg ttggcactga
    12301 agagggtgta gccagcagta tggaaaaggc agtggccggt gctgaaaggg agcagagaga
    12361 aggcgcaagt ggaaagtggg tagcacactg gaagatggtt catatagtaa gaccagtttg
    12421 ggaaaaagtt ggtgaagcta accagcttgg aagaaagttt ccgcctctta catatactga
    12481 ggaagattct caagccctca cccatttaga acctgcccca agatctataa ggggagcaag
    12541 aaacctgctt agcgtagccc tgcagtacgg aaacgcattc ggccagggct ttcaggcagc
    12601 agcgctaaaa ccagcagact tttttgaaga tgataatatt ctttatctta tggaagacgc
    12661 tgctaccgga gaaattcgcc tgagtatcct atgggaatgg atccacaagg gagcaaaatt
    12721 aaacgaagac gatgcagaga ccggcctgaa agcaggggat acttttactg tagaaatatt
    12781 caataaactt ttggaagaag agtatgaaaa gctaagaaat gccaaggata aggacgttta
    12841 tgacatttca aaagacacga ctcttcctat cgcaaaagaa attgttagaa cctatgtact
    12901 agatgaagtg aaagtaccat ggtacattga tcttcttaac atcaatttaa acaatcacga
    12961 tttgcaaaca gcaaaaaaga gaatacaaca atatatgaca gcgtttaaaa aagacggtac
    13021 aagaattacc gaaaacctgg actttgttgt atcttaaaaa aattaaataa tcaggagagg
    13081 agagcacaag aaaatgggtt caatggaaag cgaagcaaaa agaattaagt catatttttc
    13141 aagccagaga ttcaagggaa ttacaaggct gtattctgca gagcaggtag tcgcacagca
    13201 gggaacaata aaaaatgact atacaattgc taaagaggca tcaataaagt tttataaacg
    13261 ccttagagaa ctgtattcaa agaaaaaatc tattacaaca tacggacctt attccccagg
    13321 acaggtcatc atgatgaaac gcgccgggat agagggaata tatcttggcg gctgggcaac
    13381 atcagcaaag ggttcaatta ccgaagacag aggtgcagac ctagcaagtt accctctaag
    13441 ccaggtacct gacgatgcag cagtacttgt tagagctctt ctgagtgctg ataaaaacca
    13501 gcagtttgaa agatcaagaa tgacagaaaa ggccagaaag gctacacccg agattgacta
    13561 tactccatat ataattgcag atgccgatac agggcatggt ggtgacgctc aggtaagaaa
    13621 cctgattaga aggtttgttg aggtaggagt aaccggatat catattgaag accagaaacc
    13681 cggtactaag aaatgcggac atcagggcgg taaagttctg gtacctgtag acgaacagat
    13741 caaaagacta aatgcagcaa ggttccagct ggacattatg gaagtaccgg gaatcatcgt
    13801 tgcaagaaca gatgctgagg cagctaccct tcttgaaggc agaggtgatg aaagagatca
    13861 tccgtttctc ctgggtgtaa ccaacctaac cgttccggct tataaagatg cctacctatc
    13921 aattttaaaa agattttatg ataagggtat aaaagatatt aacggtcatt tgctatataa
    13981 aatctcaaat gatgtttata aatcttgtta tgcatggcta aaagatgtag gggtcttgga
    14041 gattgcggac agcaacatag aagcatataa aaaaggcaag aaaacttcac tcgacaaaac
    14101 acttgatgat gtagtgaata aatttgtaga aacatgggaa gcagaatctg ggatcaagac
    14161 atttagaaag gcagtagccg acaggatgga attcagggca gaggaaggca cagattttga
    14221 tatgacagca gagcagtggg acgaatttgc aaaacaggta tcactttccg aagcacagaa
    14281 aaaagccaag gatatgggac taaacgtcac atgggactgc gagctctcaa gaacccctga
    14341 gggatattat cagatacagg gtggaattga atacgcaatt gcaaagtctc ttgcggtagc
    14401 tccttttgca gacatcctct ggatggagac aaaaacagct gatcttgcag atgctaaaga
    14461 gtttgctgat gcaattcatg ctgtatttcc ggatcagatg ctcgcataca acctctcccc
    14521 ttcttttaac tgggatacaa caggtatgtc tgagcaggaa atgaaagatt ttccaagtga
    14581 aattggaaag cttggttttg tatttaactt tatcacttat ggtggccacc agattgacgg
    14641 tcttgcatct gaagagttct caagagctct gcttgaagac ggagcgcttg ctcttgcaag
    14701 actgcaaaga aaactaagac ttctggattc accgtacaga acaccacaaa gctatgttgg
    14761 tggtcccaga atggatgcga tgcttacagc ttcttcaggt cgtacagcaa ccacaaaagc
    14821 aatgggtaag ggatccactc agttccagca ccttgtacaa acagaagttc caacaaaggt
    14881 actggaagac tggctcgaga tctgggccaa gcattacaag attaaaggct ctctcagggt
    14941 tgagctcaga ccaaacagag cgggttctga tctgcttgaa ttaaacatct taagcagcag
    15001 atcaaaaaac aagatggccg atgtaatttt tggttccatt caggatttaa ggggcaagaa
    15061 tattatatcc ataagagatc aaaacacata cagcactgaa ttcaggcaga aacgtctcat
    15121 gacaataatc catctgttcc tgattcacag atacaaaggc gattcggttc attatgtaaa
    15181 tccaacagat gacaatatga agcaaaccca gggcatgaaa aagcttggta tttatagcga
    15241 ggttaatacc gaagttggcg acataatcgt tgccggtatt aatgctaaga aagtaaaaga
    15301 cctgctaagc gaagatcagg tggatcttaa aaaccttatc gctaaaaagg atagtgctaa
    15361 aaaaccagca gcaaaaaaga cagcttcaaa aagaaaataa gtatttaatc ttacaataag
    15421 actccaggcc gtgattacac ggtctgggga tttttagtct cctttaaagt aatccttaac
    15481 tatagtcaaa gcaattaatg aagatgctcc gatccatctt catgaatatg tacatcactg
    15541 tcttttttat cttccagtaa tgttttcata ttcttttcaa tttcacttcc atgcacatga
    15601 tcatggccat catccacatt tttattcttt aaagcatatg atttatattc ctgttcactt
    15661 aaattgggca gtacctgaag gaatgccaca attgcccaaa tctcttcatc ggaatgtgta
    15721 ggcccatatg caggcatacc ggtcatctta attccatttt tggttatcca aaatagttca
    15781 gattgtgtcc attcatcatt ttcttcatag agctcagggg gttcggggtt aagaccaata
    15841 tgaagcaccg attctggtat acctggtgct ccatgacaca ttgcacacat agagtcatac
    15901 tgaacaaacc ctgaattaat cattttttca tcgccaagat cgggagcttt aatatctttt
    15961 gcatgatttt taactgacat ttccatcgat tcatgaagta tcaattttac agccttattg
    16021 tgtggtgaag ttgcccctac attatacatt cctttataga gagcaaacgc caaacccgct
    16081 gccaaaacta ttattaataa gagtaatttt gatataaaat tcatctgacc gtcctataca
    16141 accatgttta tttactttca atttagttaa tactaaattt tatcataata taagtataaa
    16201 attactataa aatttaaata attaatataa ttttatcgtg taattcattg aaataaaaca
    16261 aaatattaat aatcatctgt agtcactaaa acatattaca acaaatatcc aattaatagg
    16321 tatataattt taaatcgttg acttttcaat acttagagct tacaatttaa gaaaactttg
    16381 taggggtgtg atattaaaat gtccaagaaa attcttctag tctgtgatga taatgaaaaa
    16441 tccgaagttt taagttctgc acttttaagc agcggttatg aaacaaactc actgtctgat
    16501 tattcagata tagtaaacaa atcagtagaa gtaaatccag atatgattat catggatatt
    16561 gatattccta ttaaagacgg atataaaata tgcagagaac taaaagaagc acctgatact
    16621 cagcatataa aagttatatt tgtaaaaaat gataataatc cactggaaaa agcctggtct
    16681 gttgtaaaag gggcagattt ttattttgct acaaaaaacc catttagtga gaataactta
    16741 attcagagta tgtctgttct taactaaact gataaaatat caggtgaggt ttaattcttg
    16801 aattaatctc tgcacttttt ccttatctat ctcatattca ctagatatac ggtcaatatc
    16861 ttcattagag atttgtaatt cagatattga tttgcttcca ataacttcag gctccagtct
    16921 gtttgttctg tttctttgca gctgtagtac atcccacctt gcatagtggc caagagaatc
    16981 aaatatggcc ttggtagcct ttatctgatt tgcataacaa tcggtgtaaa gtatagcgtc
    17041 cttttcaaag tttggttcta caaggtaccg tcctgctggg tttactatgg agcttccacc
    17101 cctggcccat gaaaaattca tatgatccga gtcccttatg tattcccacc tattgggaaa
    17161 atccttttca tccatatagc cgcagcagct gagtacaaat gcacctgctt caaaggcatg
    17221 tactttaatg agattttgaa ggttacagcc acctccaccc tcaccagtgt cggcatcaag
    17281 cagttgatca gctccccttt tccagttacc aggccataca caaacatgaa attcttcgcc
    17341 ctgatttatc attgaaaccc ttgcaagagg catatggttt tcccagcata taagcccgcc
    17401 tattctacca atctctgtat cataaacatc gaggtcactt ccatcaccca taccccagta
    17461 gaccctctcg gtatatgttg gcattagctt tctgtgcctt cccataacct gtccgtactt
    17521 atctatgaac agaagtgaat tgaacacagt acaacttccg gccctgtcat ctatttcatt
    17581 acagccaata actacatagg cccctgcact cttagccgct tttccgagaa tttcagtatc
    17641 ttctcctggg atgagaaccg agttttcctg aatagcaatc atataatctg tccactcatg
    17701 aggagcagtt tcataaccta ctgagtagta ggccgggtag cccggaataa aagcttcgga
    17761 gaatacaatt agctcagcgc cgtttttacc tgcttccttt atcagtttgc aggctttttt
    17821 tatggattta tccttatcca taaaaacggg agacgcctgt acaacagcca cttttacctg
    17881 ttctcttcca ccaagtactt tctttttcat aataattata aatatataat tacaaactta
    17941 cacgattata ttacttcctc cgggcgcacc accaggagct ccgccgggat cacctggccc
    18001 tccaccctgc tgtcttcttt taagagactg ctccagcttt ttctctatga gctcaaacaa
    18061 attatcctta aaattatcat catatgcaca aatcgatggg aagatataac ccataagcgc
    18121 tatgtttaaa aagaactccg cattagcctt taagaaggtt tcatcaattg ccgtgccata
    18181 agcatcctgc agattcttat atatctcctg aaagagctca tccccggcct cgagcacatt
    18241 ttttatttgc ccctcttcct gcatcttttt cactcccata ttaaacgcca taagcactga
    18301 gtccatcatt cccggcgctt tttgatcttg ctgattttgg ttattttctt ccattttatt
    18361 cctccgaaat aataatctat taaataaaac attttatgag taaaataaca aacagtataa
    18421 ctatatttcc aaaaatattc taaaatgaaa tcgattttag ctggaggaag gaaatgccaa
    18481 aagtaacagg aggttggctt gtagcaaaaa cccttagtga tctgggtgta aaacatatct
    18541 tcaccctgag cggtgggcat attaatccga tatataatgc atgcaggcag cttggaataa
    18601 ctctcatcga tacccataac gagcagggag catctatggc agcagacgct tatggaagaa
    18661 taacccgtaa accagcagtg tgcctgacta cagcaggtcc ggggcttaca aatgctctca
    18721 cgggtattgc gggttcttat cttgcaaact ccccctgtct ttatatctcc ggcagatcgg
    18781 gtgttgaaga aaacgacaag cttcctctcc aggaaattga tcaaaaatcc atggttactc
    18841 cggttacaaa atgggcaagg gatatttatg acataaaaag aatccccgaa tacttatcca
    18901 tcgcttacaa taaagcggta tctggcaggg caggccctgt gtatctgggc atgtcttatg
    18961 aagtgcttta tccggagata aaaaaatcag atgcaaaatc catgtattca cctgctccat
    19021 cgaacaagat agagcctgac gccgacacaa ttaaggaagc tttagaatta cttataaaag
    19081 ccaaaaagcc tgtagtcctg gcaggaagcg gcacctggta ttcagactgt gccgaacaac
    19141 tagaacagtt tattaaactc acgggaatcc ctgtttttac tctcaatctg ggaaggggaa
    19201 taatttcaga tgaaaatgag tactgtatgg gttcagcaag cccttcagcg ccaaacggtt
    19261 ttagagatat tacaggtaaa gcggatttaa tattaatgct gggtattaga ctaagcctct
    19321 atataggttt tggaaagaca tttaatccaa aagcgaaaat tatacaggta gatatacaag
    19381 cagatgagat aggaaggaac agggctgcgg acttaagcat tgttgcagat gccggaaagg
    19441 ttttagaaaa atgcatagat gaagcagatc agcagggagt tcaatttaat tacaagaagt
    19501 ggttaaaaaa cgcaaaaaat attagaaatg atgcatggaa agaaaccgcc aggataagaa
    19561 aatcaaataa gaagccgatc catgcattaa gggtaataaa ggctgtcgaa aacgtaatga
    19621 gggaagatgg catgatcgta attgatggtg gagacactca ggcatttagc gacggtactt
    19681 ataatgtgag acagcctggg cattacataa aaggcgggcc tcttgggtgc atgggtactg
    19741 gaattccatt tgctcttgga ataaaagcag catgtcctga taagcaggta ggtctaatca
    19801 caggcgatgg agctttcggg atgaacttca tggaatttga gacagccgta agacacaacc
    19861 tgccttttgt tgctgtagtc tgcaatgatg agggatgggg aatgacaaag catcagatgt
    19921 ggatctcatt tggaaagaaa accccaaccg ttggagtcga tcttcctttt accccgttcc
    19981 atgagcttgc aaatgttctt ggaggttatg gtgaaatggt tacagacccc attaaccttg
    20041 aaaaagctat taaaagaggt cttgattccg gagtacccgc tattataaat gtaaagaccg
    20101 accctgaggc tatcagcagg gcaacatacg ggcttacaga gatgatgact ccaaaggatt
    20161 agttgccagc atgaaactta gactacccgc agagtgggaa aaacatgagg ctacatggct
    20221 tgcgtggcca aagaggcgag aagactggcc gggcaagtat agctctatca aatgggtcta
    20281 tgctgatata ataaaagcaa tatcttattc tgagactcta aatctaatcg tagattcgga
    20341 acaagccgag caaaaagtaa gagctattct aaaaaaatcc tcagtcgatc tgaatagaat
    20401 taaattctat cacattaata cagataggtc atggataaga gattatggcc ctttttttgt
    20461 gaggttacaa aataaaataa atgctctaag ctttaagttt aacggctggg caaagtattc
    20521 acagtggaaa aatgacaaca aggttgcttc ccatataaca tctaatctaa acatagagag
    20581 tataagagca gaatattaca acaaagaaat tatacttgaa ggcggaagca tcgatgtaaa
    20641 tggaaaaggc actcttataa caaccaggca gtgtcttcta agctcctcaa aacaggtgag
    20701 gaaccggggc cttagcaaag aggattatga acagatattt aataattatt ttggtattac
    20761 caacactatc tggctaaatg aaggaatcgc aggtgacgac accaacgggc atattgatga
    20821 tatatgcagg ttcgtaaata ctgacacagt tgtagcatgt catgagtcta atccggatga
    20881 tacaaattat aaatacttaa aagaaaatct ggaaatactc ggagagagca ggcttgagga
    20941 cggctcaaag attaatgtta tcaagcttcc catgccagag cctaaatact ttgatggtta
    21001 cagactgcct gcaagttatg ctaattttta tatcaccaat aaatgcgttc ttgtacctac
    21061 atataatgat acaaatgatt acaaagcatt aggaatcctt ggtgaactgt tttcagatag
    21121 aaaagtcatc ggaataaact gtgttgattt aatatggggt cttggagcta ttcactgtat
    21181 gacaaaagaa cagtttgctc agtcatgaat cgatctggtc tgcaagaacc tctgctatat
    21241 gttttgtttt tcta
//
LOCUS       DBNGSV010000244         1964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_112358, whole genome shotgun sequence.
ACCESSION   DBNGSV010000244 DBNGSV010000000
VERSION     DBNGSV010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_112358"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="AC159U_09070"
     CDS             <1..362
                     /locus_tag="AC159U_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337249.1"
                     /translation="QTVTNQIAVAINNAQKAEELKISKRSLSINLEQINKKQHYDEII
                     STVVKSVHSSIDLQQVFDNTVDALIKNIKIINHAALHIVEDDVAIMKAYRGHPKWFVN
                     IVKRLERPRGFTWKTII"
     gene            384..1103
                     /locus_tag="AC159U_09075"
                     /pseudo
     CDS             384..1103
                     /locus_tag="AC159U_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
     gene            1260..>1964
                     /locus_tag="AC159U_09080"
     CDS             1260..>1964
                     /locus_tag="AC159U_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3337250.1"
                     /translation="MFKSAADSLVENLHKVDNVSIFLVEGDRAVNKAYKGFPKWFIDI
                     TKSIPYPRGYTWDTIIKGKSRYCSNVDKDKSIGPAGRKVGIKSYFTVPFKHDNKTIGC
                     INFNSKTPNAFTSEELILLENITIQIESAIKNAFQAEAVKESEQRFRNMADTAPVMIW
                     MSDRNKQCTYVNRNWLEFTGRSLQKELNKGWQNIVYKDDFEKVLKICDESFSKRKEFK
                     IEFRLKRHDGQYRWIFD"
BASE COUNT          782 a          260 c          354 g          568 t
ORIGIN      
        1 ttcaaacagt aactaatcag atagctgtag cgattaataa tgcccagaag gctgaagaat
       61 tgaaaatttc taaaagatct ctatcaataa atttagaaca aataaataaa aaacagcatt
      121 atgatgagat tataagtact gtagtaaagt ccgtacatag ttctatagat ttacagcaag
      181 tatttgataa taccgtagat gcattgataa aaaatattaa aataattaac catgctgctc
      241 ttcatatagt agaagatgat gttgcaataa tgaaggctta taggggtcac ccaaaatggt
      301 ttgtgaatat tgtaaaaagg cttgaaaggc ctagaggatt tacttggaaa acaataatct
      361 aaggtaagtt aaattattgt cccgatatag ataaagacaa atcaattggt cctgctggaa
      421 gaaaagttgg aactaagagt tatgtctctg ttcccattac catatcagga aatactattg
      481 gttgtataaa tgttcattct aaacatatag atccatttgg aaaagatgat ctcgaattgc
      541 tggaaaatgt tactaatcag atagcagtgg ccattgataa tgcaagacag gcagaaagat
      601 taaaaatttc tgaaaaatcc ttaaaagata atatcgatat tttaacaaaa aaacagcgac
      661 ttgatgaaat atctaatact ttattaaaaa gcgttcataa atcattagat ttaaagacta
      721 ttatgcataa tgctgtattc gctataacgc agaacatgcc tgcagttgaa aatattatga
      781 tttttttagt tgaaggtgat aatgccgtgc ttcaggcaca taaaggatat cctgactgga
      841 tgataaatag attaagtgta ataccaaagg gaaaaggatt cacatggaag gctttatcta
      901 gcggcaaatt tttatatgta agagatgctg aaaacgataa agtaattgga gaagctggta
      961 aaaagcttgg gactaaaagt tatattgccg ttccaattaa gcttggtaga aagaccatag
     1021 gagttattaa tataaattca ctacaaaaaa atgcatttga tatagatgat attgcagtac
     1081 tcaaaaaaat tcctaaacag ttagaaatag ccattaataa tgcaaataaa gctgaggcac
     1141 tggcaaaatc tgaaaaaaaa ttaaaggaaa atattttaat tcttaaggat aaaagcaaat
     1201 acgacacaat tatcagaact atttctacta acgtacatga atctatagat ctacagacag
     1261 tgtttaaatc agcagcagat tcactcgtag aaaaccttca taaagtcgat aatgtttcaa
     1321 tatttttggt tgaaggagac agggctgtta ataaagcata taaaggattt cccaaatggt
     1381 ttatagatat aacaaaatca attccttatc ccagagggta tacgtgggat actattatta
     1441 aaggtaaatc aagatactgc tctaatgtag acaaggataa atctataggt cctgctggaa
     1501 gaaaagtagg aataaagagt tatttcacag ttccttttaa gcatgataat aaaaccatcg
     1561 gctgtattaa ttttaattca aaaacaccga atgcgtttac cagcgaggaa ctaatcttac
     1621 ttgaaaacat aacaatacaa attgaatccg ctattaaaaa tgccttccag gcagaagctg
     1681 taaaagaaag cgagcagcgt tttagaaata tggcagatac tgcaccggtt atgatctgga
     1741 tgtctgatag aaataagcag tgtacatatg taaacagaaa ctggcttgaa ttcacaggta
     1801 gatcactaca aaaagaatta aacaaaggtt ggcagaatat tgtttataag gatgattttg
     1861 aaaaagtctt aaaaatatgt gatgaatctt tttctaaaag aaaagaattt aaaatagaat
     1921 ttagacttaa aagacatgat ggtcagtata ggtggatatt tgat
//
LOCUS       DBNGSV010000245         6132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_459043, whole genome shotgun sequence.
ACCESSION   DBNGSV010000245 DBNGSV010000000
VERSION     DBNGSV010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_459043"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="AC159U_09085"
     CDS             complement(<1..1049)
                     /locus_tag="AC159U_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3337251.1"
                     /translation="MFNRTNFIKGLVVALIIVSFSIPYLLLMVEIKKDVYNFDHFNWL
                     ISLGKFFVYSLFAPLVFLLLLSIAKQKKILTKVSAVLYIIVYFAALLFILIYYKYFGF
                     IPHYKVLSRAYEGSHLIRQVYSQLLGYEEWAIIILLFASIWLTFKYLNLPSAMRIKSY
                     IKVLMIVVVAVGNVALVAFQNFRYGSSDVLVKLGSSAAVLHLGVLPVYKEIFSYEMSY
                     EKKDYPYPGKINKDNVRQENIARFENANVIIVQIESLEKQAVDYLINGKLVMPFLNKF
                     KNKSVYFTNFFAQHSGGASTDADLGLLASLLPLRNQVGLFSADYSRITTIVEVLKENG
                     YTSFAMNPIDGQFFN"
     gene            complement(1184..1267)
                     /locus_tag="AC159U_09090"
     tRNA            complement(1184..1267)
                     /locus_tag="AC159U_09090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1231..1233),aa:Leu,seq:caa)
     gene            1398..1613
                     /locus_tag="AC159U_09095"
     CDS             1398..1613
                     /locus_tag="AC159U_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3337252.1"
                     /translation="MLKGKVKWFNNSKGYGFIVPDDGGNDVFVHYSSIEHEGYKVLNE
                     GEDVEYETTDGPKGPQASKVVRVSKSE"
     gene            1945..2340
                     /locus_tag="AC159U_09100"
     CDS             1945..2340
                     /locus_tag="AC159U_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3337253.1"
                     /translation="MNIFKLSRILKVSAIFVFMTLVLNANSYAEGDAVKGKESYDQIC
                     ASCHGMSGKGDGVAAAALNPKPRDLSSAEYVSTLADDYIFKVIKEGGASVDKSPLMPA
                     WGGVLSDDKIWNVIAYIRNDLCKCEAKAK"
     gene            2363..2455
                     /locus_tag="AC159U_09105"
     tRNA            2363..2455
                     /locus_tag="AC159U_09105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2397..2399,aa:Ser,seq:gga)
     gene            2469..2566
                     /gene="ffs"
                     /locus_tag="AC159U_09110"
     ncRNA           2469..2566
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC159U_09110"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2579..4222
                     /gene="dnaX"
                     /locus_tag="AC159U_09115"
     CDS             2579..4222
                     /gene="dnaX"
                     /locus_tag="AC159U_09115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3337254.1"
                     /translation="MSYIVLARKWRPQNFGEVIGQQFITTTLKNSLSSGKIAHAFLFS
                     GPRGVGKTSTARILAKSINCEKGPTPDPCSKCTNCTEISAGRSVDVIEIDAASHTGVG
                     DVREIIENIKYMPVSGKTKVYIIDEVHMLSNSAFNALLKTLEEPPEHALFILATTEAS
                     KIPLTILSRCQKYDFKKVTSKEIKESLTHITTKENIEIDEQSLHMIASESDGSLRDAL
                     SLLDQLNTTFDGRIEFEQASKLFGIFDSSYTLSLFKNILAKDPKACLEIIIELGQKGI
                     NPKKTAEEILRIARHSVHIKACGTHISLDLTEDEISSIDKEVQSFELAALENIFNQAL
                     RACEDISKSAYPQIALEMNVVKLATLEHAISVDEILDRLQGSESNTPAGHVRSSPKPK
                     TTREPSDAAAVAPKKIVTEIKEQADSELSVSTFIEYIKEHASMTGIYLERADKIEIED
                     TVIRLTYKSQSVFSDSIQKADNIDSIKSLLHKKYSKDFSIKVLVNKSGKEPQTETKNE
                     YEQKDQGTNVMNNKSLKEALDTFGGRIVKVKNNKKGEDQ"
     gene            4219..4539
                     /locus_tag="AC159U_09120"
     CDS             4219..4539
                     /locus_tag="AC159U_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3337255.1"
                     /translation="MKLGNIQDMLKQAGNIKQQMEQIQNEAGEKVVEATSGGGMVTVV
                     AKAKGEIISIKIEPTLLADPDIEMIQDLITAACNEALNRARQVMKEEVSKVASNFGLP
                     PGLI"
     gene            4556..5155
                     /gene="recR"
                     /locus_tag="AC159U_09125"
     CDS             4556..5155
                     /gene="recR"
                     /locus_tag="AC159U_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3337256.1"
                     /translation="MNEGFPKPISNLIELLSQLPGIGEKNATRLAIQILKNSVEYSYS
                     LSNAITEVKSKVNLCENCFCLTAESLCLFCSSEGRANIICVVEDPIDLIAIEKSNEFR
                     GRYHVLHGTISPIDGIGPDELRIKELLQRIESENIEEIIIATNPSVEGEATSLYLSKL
                     IKPLGTMITRIAHGVPMGGDIEYIDELTLGKAIKDRRPI"
     gene            5266..6075
                     /locus_tag="AC159U_09130"
     CDS             5266..6075
                     /locus_tag="AC159U_09130"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3337257.1"
                     /translation="MIDTKSLENTWAVILGSSSGFGEALCLELADCGMNIFGIHLDRK
                     GTMPKVEEIINKIKSKGRKVKFFNINVSDAEKRAQTIEEISNTLKEENNPGSVRVLVH
                     SVAFGTLRPYIDEEKKKMVTDTNMNMTLDVMAHSLVYWAQDLISNGLMGEGGSIFALT
                     SAGGHRIWPTYGPVSAAKAAIESHIRQLAVELAPKRITANSIMAGVTETPALKKIPGN
                     EEIVEKASKYNPSKRLTTTQDVAEAIAILSLPQARWITGNLIGVDGGEDIV"
BASE COUNT         2115 a         1133 c         1250 g         1634 t
ORIGIN      
        1 ttattaaaga actgaccgtc tatgggattc attgcaaaag atgtatatcc attttctttt
       61 aaaacttcta caattgtagt aattcggctg taatctgctg agaataggcc cacctggttt
      121 ctaaggggca ggaggcttgc caacaaaccc aggtcagcat ctgtagaggc gccgccgctg
      181 tgctgagcaa aaaaattagt gaagtaaacc gacttatttt taaatttatt caaaaacggc
      241 attacaagct ttccgtttat taggtaatca accgcctgtt tttcaagtga ttctatctgg
      301 acgattatta cattagcatt ttcaaatctg gcaatgtttt cctgtctgac attatcttta
      361 ttaatctttc ccgggtatgg gtaatccttt ttctcatatg acatttcata tgaaaagatt
      421 tctttatata ccggcagcac tcccaaatgc aaaacagctg ctgaggagcc taatttcaca
      481 agaacatcac tacttccgta tctaaagttt tgaaatgcta ccagtgcaac atttccaaca
      541 gcaacaacaa caatcattaa aacttttata taactcttta ttctcattgc gcttggaaga
      601 tttaggtatt taaaggtaag ccagatggaa gcaaacaaaa gaataataat tgcccactcc
      661 tcataaccca gcagctgaga ataaacctgg cgtataagat gagacccctc atatgccctt
      721 gaaagaacct tataatgcgg aatgaatccg aagtatttat agtatatgag tataaatagt
      781 agagcagcaa aatagacaat aatatataaa accgcagata cttttgttag aatttttttc
      841 tgtttggcaa ttgataataa taaaagaaaa accaggggtg caaagagcga gtaaacaaag
      901 aatttaccta gagatatcag ccagttaaaa tgatcaaaat tataaacatc tttttttatt
      961 tccaccatta gaagtagata tggaatggaa aaacttacta ttatgagcgc tacaacaagt
     1021 ccttttatga agtttgttct attaaacatc tctgaatttt aagacattta ttggattatt
     1081 gttactactg agagatacaa taacaggcaa taaatacaat aattagtaaa aaaattttag
     1141 ataaccaaaa ccaggttcaa ataatctgaa tgtgctaata ttctgggcag agagggactc
     1201 gaaccctcac gagcgtaagc ccaacggctt ttgaggccgt cgcgtctgcc aattccgcca
     1261 cctgccctgc tgcaattaaa gagatatcta acacataaaa agtttgcatg tcaaattact
     1321 taagctacct gccagcattg acttattgat caatttgtga gatattttta aggactgaga
     1381 tttaggaggt aacttagttg ttgaaaggta aagtaaagtg gtttaacaac tctaagggtt
     1441 atggttttat tgttcctgat gacggtggta atgacgtgtt tgttcactat agctccatag
     1501 agcatgaagg ttataaggtg cttaacgaag gtgaagacgt tgagtatgaa acaactgacg
     1561 gcccaaaagg ccctcaagca agcaaagtag taagagttag caaaagcgaa taactttttg
     1621 cctgttttta gttgttattt ataaagtttt atgattttgg aagttaacct tcctggttat
     1681 tccgggggtt tcttttaata tctgagattt tatctatctt tttcttcttt tatagtacag
     1741 ttatattcat tttccttttt agagcactat tagatattac tctttgctcg actcttttat
     1801 cttttgaaac tagattgaaa tttcggagtt tgtaattaat atcattgtaa atagactccc
     1861 actttgcatt aaaaccaaat tggtatttaa aaatacgaat atactgttag aataggtggt
     1921 tgttttaata tagaggaggt tttaatgaac atatttaaac taagcagaat tttaaaagtt
     1981 tctgcaatat ttgtttttat gacactggtt ttaaatgcaa actcttatgc agagggtgat
     2041 gcagtaaagg gcaaagaatc atatgatcaa atatgtgcat catgccatgg catgtctgga
     2101 aaaggcgacg gagttgctgc agcagcatta aacccaaagc caagagacct tagcagtgca
     2161 gaatatgtat ctacacttgc tgacgattac atatttaaag taataaaaga aggcggcgct
     2221 tctgtcgata aatctcctct aatgcctgca tggggcggtg tattatcaga tgataaaatc
     2281 tggaacgtta ttgcatatat cagaaacgat ctatgtaaat gtgaggcaaa ggcaaagtaa
     2341 gtatttgaaa ataaaatatt aaggagaggt gaccgagtgg ctgaaggtgc acgcctggaa
     2401 agcgtgtaag gggtttatag ctccttcgag ggttcgaatc cctccttctc cgccaataca
     2461 aattcaacca gtcgggcccg tacggcagga acttgtaaac cccgccaggc ccggaaggga
     2521 gcaacggtag caagagctcc agcgcgttgc ggttacctga ctggttgttc ataaaaaaat
     2581 gtcctatata gtacttgcaa gaaaatggag accacaaaac ttcggtgaag tcataggaca
     2641 gcagtttata acaacaaccc taaagaattc actatcatcg ggcaagattg cccatgcatt
     2701 tttattctca ggccccaggg gcgtaggtaa aacatccacg gcaagaatcc ttgctaagtc
     2761 cataaactgt gagaagggcc ctacccccga tccttgttca aaatgtacta actgtaccga
     2821 aatatcggca ggcagatcag tcgatgttat tgaaatagac gcagcctctc atactggggt
     2881 tggtgacgtt agggaaataa ttgaaaatat taagtatatg ccggtttcag gaaagacaaa
     2941 ggtttatatt atagatgaag tccacatgct ttcaaactcg gcgttcaatg cactgttaaa
     3001 aacccttgag gaaccccccg agcatgcact tttcatactg gctaccactg aagccagtaa
     3061 aatccctctt accattcttt ccagatgcca gaaatatgac ttcaaaaaag tcacgtccaa
     3121 agagattaaa gagagcctta cacatataac aaccaaagag aatattgaga tagatgagca
     3181 atcgctgcat atgatagcat cagaatctga cggaagcctc agggatgctc ttagtcttct
     3241 tgatcagtta aacacaactt ttgacggcag gatcgaattt gagcaggcct ctaaattgtt
     3301 tggtattttt gacagctcct acaccttaag tctttttaaa aatatcctcg caaaagaccc
     3361 taaagcctgt cttgaaataa ttattgaact tggtcaaaaa ggcataaacc caaagaaaac
     3421 agctgaagag attcttagga tagcaaggca cagtgttcat ataaaagcat gcggcacaca
     3481 cataagtctg gatcttaccg aggatgagat atcatccata gataaagagg tacaaagttt
     3541 tgaacttgca gcgcttgaaa atatttttaa tcaagcactt agggcttgcg aagatataag
     3601 caaatctgct tatccccaga ttgcacttga aatgaatgtg gttaaacttg ctacactgga
     3661 acatgcaatt tcagtagatg aaattctgga caggctgcaa ggctcagaaa gtaatacacc
     3721 agcaggtcat gttaggagct cacctaaacc taaaaccaca agagagcctt cagacgctgc
     3781 agcagtagct cctaaaaaaa tagttacaga aattaaagaa caagcagact cagagttatc
     3841 ggttagcaca tttattgaat atattaaaga acatgcaagc atgacgggca tataccttga
     3901 gagagcagat aaaatagaga ttgaagatac ggtgataaga cttacctata aatcgcagtc
     3961 tgtgttttca gacagtattc aaaaagcaga taacatagac agcattaaaa gtctgctgca
     4021 caaaaaatac tctaaagatt tcagcatcaa agtacttgta aataagtcag gcaaagagcc
     4081 tcagacagaa acaaaaaatg agtacgaaca aaaagaccag ggaacaaatg tcatgaacaa
     4141 caaatcgctc aaagaagcgc tggacacttt tgggggcagg atagttaagg tcaaaaacaa
     4201 caagaaagga gaagatcaat gaaacttggt aacatacagg acatgctaaa acaggccggc
     4261 aacataaagc agcagatgga gcagattcaa aatgaagccg gcgagaaagt agttgaagca
     4321 acttcagggg gtgggatggt aacagtggtt gctaaggcaa agggtgaaat catctctata
     4381 aaaattgagc ccacgctttt agcagatcct gatatcgaaa tgatacagga ccttataaca
     4441 gcagcctgca atgaagctct aaacagagca aggcaggtaa tgaaagaaga agtgagcaag
     4501 gttgcaagca atttcggtct gccgccgggg ttaatttaat ccgtactgca atatcatgaa
     4561 cgagggattt cccaagccta tatcaaacct catcgagctt ctctctcagc ttcctggaat
     4621 tggtgaaaaa aacgccacca gacttgccat tcagattcta aaaaactctg tagagtattc
     4681 ttatagtctt tcaaacgcta ttactgaggt aaagtcaaag gtcaatctgt gtgagaactg
     4741 tttttgctta acggcggaga gtttatgttt attttgcagt agtgagggca gagcaaatat
     4801 aatctgtgta gtagaagacc ctattgatct gatagcaata gaaaaaagca atgagtttag
     4861 aggaagatac catgtgcttc atggcactat ttccccaata gacggtatag gacctgatga
     4921 gcttagaata aaagagcttt tacaaagaat agaaagcgag aatatagaag agattattat
     4981 tgccacaaac ccaagcgtcg aaggtgaagc cacttcactt tatctgtcta agctaataaa
     5041 accacttggc acaatgatca caagaatcgc tcacggtgtg cccatgggag gggatataga
     5101 gtacattgat gagcttacgc ttggaaaagc aataaaagac agaagaccaa tataaatatc
     5161 cccttagtaa tattataagc taaacccctt gaaataagcc gcaaaaaccg tagaatctga
     5221 tctacctgtc attaagcata actaacaaaa tatggaggcc ttaaattgat tgatacaaaa
     5281 agtttagaaa acacatgggc agtaatcctc ggctcatcca gcggttttgg agaagcgctc
     5341 tgcctggagc ttgcagactg cgggatgaac atctttggta tacacctcga tagaaaaggc
     5401 acgatgccaa aggttgaaga aattataaac aaaattaaaa gtaagggaag aaaagtaaag
     5461 ttttttaata ttaacgtatc cgatgctgag aagagagctc agacaatcga agagatatca
     5521 aatacactaa aagaagaaaa caaccctggt agtgtaaggg tgcttgttca ttctgtagca
     5581 tttggaacct taagaccata tatagacgaa gaaaaaaaga agatggtcac cgataccaat
     5641 atgaacatga ctcttgacgt aatggctcac agccttgttt actgggctca ggaccttata
     5701 tcaaatggtc ttatgggaga aggcggcagc attttcgcac tcacaagcgc gggtggtcac
     5761 aggatttggc ctacatacgg gcctgtttca gcagcaaagg cagcaattga atctcatatc
     5821 agacagcttg cagtggagct tgctccaaag agaataactg caaattctat aatggctggg
     5881 gttacggaaa cacccgctct taagaaaatc cctggaaatg aagagattgt tgaaaaagca
     5941 agcaaataca atccaagcaa aagacttact accacacaag acgttgccga agcaattgca
     6001 attttatctc ttcctcaggc aagatggata accggaaacc ttattggcgt agacggcgga
     6061 gaggatatag tctaaataca agaataagat gctaaaaaaa gttaaaacct ataacaccct
     6121 ttccggcaag aa
//
LOCUS       DBNGSV010000246         7377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_390274, whole genome shotgun sequence.
ACCESSION   DBNGSV010000246 DBNGSV010000000
VERSION     DBNGSV010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_390274"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..3208
                     /locus_tag="AC159U_09135"
     CDS             332..3208
                     /locus_tag="AC159U_09135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3337258.1"
                     /translation="MKTNKVSSQIHFRDLVKRTNYDLDDESLYFLLPDIPATVQAEHV
                     FIENGGIWGDRLITFAKLSAIVNLNTPNPPNIISRTARLLLAEHTVSEIAQNLKYYED
                     IVNITEFADSILRIIAELKHTNTGPHILDSISKQTDSDTLSFKLGDICMIYSAYDNKL
                     KSLNSIDDIDSLRLLIENANPSLLSSSLPQLKKLVVFGFHEFTDTQLDVLKCLDNNNI
                     EVELYEYETGSSTDQKEIRLRSYASKIAESQSCSKHIWWLINKENVPPSNIAVVCKGS
                     YENSLILKEEFKRVGLEYTEKAKISLKTSHIGKLIDSIISLKLSNFSKVSLFELLRNP
                     YIIHFFGNDSEFNSFIADLELLMLKKRITGGLEKLSKCDEITNINSYSSYFNNLFVLL
                     EKINKSFNSKKVSVLNDNLLYLLQELVFDRMKGNQFGKTESERNAVNSTLSILSELRY
                     LVQSYSSNRRVERLVDYSSIISDLLSDKNYALTSAHEENKIKILDYLEARGTVYEYIF
                     ALGLSENIVPNTDVSDPLIKNNERYFINNITKNKIFKLNNEHIESEENLVNIVLSGSK
                     HVFASYVSQDNKGRAVQPSYVLDGFKIVKDDIESDKKIITEHDKYKYLISRLDNAPNA
                     YLENENRFIINGIVSEKTRDTKFAKLTENEGLVTDLEYLQSINKFSVTELETYGSCPF
                     KYFASQILKLKYPEEIEDDPRPLDKGSVYHKLLNIIFIELSNIYGSRLDLRKVKDEQI
                     LSKLNEILESAELEKEFDWLSDFKKQLNIKSISQSVMPAFIIYEAGRIREHNNEGFFP
                     VEYEKELSLQIDDKTVEGKADRVDLSSDKAYVIDYKLSNVKNKKFCDYNNLQLPLYLN
                     ALGSNGIGKQGAYYRSTERPGEEKGKNENSKDYEKDLEVSIEFARLYMQNIQNGIFPA
                     APRQKDLDFFEFPIQLTKQTGPPCSFCEYNDLCRAKGAVLRTTN"
     gene            3215..6217
                     /locus_tag="AC159U_09140"
     CDS             3215..6217
                     /locus_tag="AC159U_09140"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3337259.1"
                     /translation="MKRISQLLSLNTQQQKLIDIDKPYVVQAGAGSGKTRVLVSRYLQ
                     ILESGANANEIVAITFTINAASEMKERIRKYIREYIDNFGETKSINYNCFKDIADSPI
                     STIHGFAAAIIRENPYECNLGSRIKILEGLDRKLFVQESVDEFLINKSQKDKNATEIF
                     KNENYNYASVKNKLIEILELTFRHHINIYDLKADDKDLKAKIDKLYKKLSSEIKLGPY
                     GSENNKINTKLKEFHAFINDLGNCELRIDKIKKLSKACQLLSNTASPKGILQLNNSND
                     LERHFAANCAEIINQIINLMELSNSRSYMDLFLKFYEFYKEKKRLIGYLEFDDLLRIC
                     LNLLKNNPDILQEYQNRFRYFLVDEFQDTDDLQYELINLFSSSNHFIVGDPKQSIFGF
                     RGASPEIFKKLQKNSDNSSLTYNYRSDYRLIAFYNELFKVIIKESYQKMEVESEGSTA
                     SARVKEILYTNVSKDETRVLVESASISRKVAELLAQGYKCKDIAILLRSKSHISSFES
                     ALCEASIPYYSSEQAGFYRYSEIRDIVCMLKYINNKNDRISEASVLRSTFAGVSDTEL
                     FNYYVKDKPSELITDYLEFVSQLRDESAVLRPVELLTRILNKTRFWEAMLALADGKRK
                     YDSITKLVELFSQLENQGKSLTEIIHFLDLNYEENTEGLSQQELDESDTVKILTVHKA
                     KGLEFAVVILADINHGMGGGSETVSFSKKDGVIVRNTGIKSKLWKEIEEEQKKNTYEE
                     EKRSLYVAITRAKEKLILSVCPDSRLNKGSYMDILNSVLSLTKIEKDDKILNFKNFKI
                     PVVRASEAIYTIPVVNDDEKEIIYEPELPIIYEIDVAVNDETLNEIDPPDLSIKLNNI
                     QIGSVMHRYLQIWNYSQHSIKSTINYVLNEYFILDSKMEELLIQLSDNYLNSDLFAQI
                     KEAEEIKREFPFYIELDGKHERRKIDLLVFKDDNIFLYDYKLSSEVKPEHIDQMDLYE
                     KALINKYNCNNISKNLVHVPDVIIKNLN"
     gene            6228..>7377
                     /locus_tag="AC159U_09145"
     CDS             6228..>7377
                     /locus_tag="AC159U_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3337260.1"
                     /translation="MEHIQKYLDYFNNIDYAQKSVDFGAEIGLFIGICVLAYIANYVA
                     KNFILRAVEGLIRKSKTDWDDILVERKVLRRLSHIAPALIFYFLVPLLFKGNESVKIL
                     TQRLASIYMLGCGLLTVDACLSAVQDIFGKFQISKQIPTSIFIQVIKILLYLLAAVIV
                     ISILIGKSPAFLIGSLGAVTAILMLVFKDPILSFAAGIQLSSNKMVQVGDWIEMPKHG
                     VDGDVIELSLTTVKVQNFDKTIVTVPPYALVTESFKNWRGMSESGVRRIKRAINVDIS
                     SVTFCSEQMIERFKKIQYISGYVDEKRTELTEYNKKQNIYDTDLANGRRMTNIGTFRA
                     YIVSYLKEHPKISKNHTLLVRQLDPGPTGIPIEIYVFTTDNNWVNYEGI"
BASE COUNT         2666 a         1150 c         1391 g         2170 t
ORIGIN      
        1 gtgttgaaat ttaaatttgg tgttatattt tattgatata caagcttttg ggagtaaaaa
       61 taattgaaga aaacaatact aaaaacttta atagtagttg gaatagggat cgggatattg
      121 atggttcctt ttttcataga caatgagcct cagcttattg ctgaacagat cacggcgaaa
      181 tagcattacc tttaaagata tgcttttttg catagtagtt attaaccgca gcttagaaat
      241 ataatcaatt tacacaccag ttttatagat atttttctca accactatat attcatagta
      301 aataatctaa ccttatttac cctattggaa attgaagact aacaaagttt cttcacagat
      361 tcatttcaga gatttagtaa agcgtacaaa ttatgatctg gatgatgaat ctttatactt
      421 tttactccct gatattccgg caacggttca ggcagaacat gtatttatag aaaacggggg
      481 tatttggggt gacaggctta tcacttttgc aaagctttca gcaattgtaa atcttaatac
      541 tccaaaccca ccaaatatta tttcaagaac agcaaggctt cttttagccg aacacactgt
      601 ttctgagata gcgcagaatc ttaaatatta tgaagatatt gttaatataa cagagtttgc
      661 agattcaata cttagaataa tagcagaact taagcacacc aatacaggtc ctcatatact
      721 ggattcgata tcaaagcaga cagactctga tacattatca tttaaactag gcgatatttg
      781 catgatctac agcgcttatg ataataagct taaatcgtta aactcaatag atgatataga
      841 ttcccttaga cttctaattg aaaatgctaa ccccagtcta ctgtcttcga gtttgcctca
      901 attaaaaaag cttgttgttt ttggattcca cgagtttaca gatactcagc ttgatgtgct
      961 aaaatgcctt gataacaaca atatagaagt agagctttat gaatatgaaa caggcagctc
     1021 tacagatcaa aaagagataa ggctcagatc gtatgcttca aaaattgccg aatcgcagtc
     1081 ttgttcaaaa catatctggt ggctaattaa taaagagaac gtgcccccat ccaatatcgc
     1141 agtggtttgt aaagggagtt atgaaaactc tctaatatta aaagaggaat ttaagagagt
     1201 tggattggaa tataccgaaa aagcgaaaat ttcactaaag acaagccata taggaaaact
     1261 tatagattcc attataagtc tcaaactttc taatttttca aaagttagtc tttttgaact
     1321 acttagaaat ccttacatta ttcatttttt cggaaatgac tctgagttta atagcttcat
     1381 agcagactta gaactgttga tgttgaaaaa acgaataact ggggggcttg aaaaattatc
     1441 aaaatgcgat gaaattacaa atataaattc ttattcaagt tattttaaca acctttttgt
     1501 actactagaa aaaatcaata aaagttttaa ttcaaaaaaa gtctctgttc taaatgataa
     1561 ccttttgtat ttgctgcagg aattagtgtt cgatcggatg aaaggcaacc aatttggtaa
     1621 aactgaatca gagagaaatg cagttaattc tacattaagc atcttaagcg aattgaggta
     1681 ccttgtacaa agttacagta gtaatagaag ggtagaaagg ctggtagatt attcaagtat
     1741 tatatccgat ttgctcagtg ataaaaacta tgctctaacc tctgctcatg aggaaaacaa
     1801 gataaagata ctggattatc tagaggccag ggggacagtg tatgagtata tatttgctct
     1861 gggactttca gaaaatattg ttccaaatac cgatgtttct gatccattaa ttaaaaataa
     1921 tgaaagatat ttcataaata acattactaa aaacaaaata tttaagttaa ataacgaaca
     1981 tatagagtct gaagaaaatt tggtaaacat agtactcagt ggctcaaaac atgtgtttgc
     2041 aagctatgtg agtcaggaca acaagggcag ggccgttcag ccttcttatg ttctggatgg
     2101 tttcaagatt gtaaaagacg atattgagtc ggataaaaaa ataattactg aacatgacaa
     2161 atataagtat ttaatatcca gattagataa tgcacccaat gcttatctcg aaaatgaaaa
     2221 caggtttata attaatggga ttgtttcaga aaaaacaaga gatactaaat ttgcaaaact
     2281 tacagaaaat gaaggacttg ttaccgatct tgaatatctg caaagcataa acaagtttag
     2341 tgttaccgag cttgaaactt atggctcatg tccttttaaa tattttgcat cacaaatctt
     2401 aaagctaaaa tacccagaag aaatagaaga cgacccaaga ccgctggata aggggtcagt
     2461 ataccataaa ctgttaaaca tcatatttat tgagctatcc aatatttatg gcagcaggct
     2521 tgatttgaga aaggtaaaag atgagcaaat attatcaaaa ttaaacgaaa tattagaatc
     2581 tgccgagttg gaaaaagaat ttgactggct ttctgatttt aaaaaacagt tgaacattaa
     2641 aagcatttca cagagtgtaa tgcctgcgtt tattatttat gaagccggta gaattaggga
     2701 acacaataac gagggatttt tccctgtgga gtatgaaaaa gagctatctc tgcaaataga
     2761 tgataagact gtggaaggca aagcagacag ggttgatttg tcttccgaca aggcttatgt
     2821 aatcgactat aaacttagta acgttaaaaa caaaaaattc tgtgattata ataatctgca
     2881 gcttccacta tatctaaacg cattgggctc caatggaatt ggaaagcagg gcgcgtatta
     2941 caggtctact gaaagaccgg gtgaagagaa aggtaaaaat gaaaacagta aagactatga
     3001 aaaagattta gaggtatcta tagagtttgc aagactctat atgcaaaata tacaaaacgg
     3061 aattttccct gcggctccca ggcaaaaaga cttggacttt tttgaatttc cgatccagct
     3121 tacaaaacaa acaggccccc catgcagctt ctgtgagtat aacgatttgt gcagggcaaa
     3181 gggtgcagta cttaggacta caaattaacc agttatgaaa agaatctcac aactactctc
     3241 attaaacact cagcagcaaa agctcataga tatagataag ccttatgtag ttcaggctgg
     3301 ggcaggatcg ggaaagacaa gagtgcttgt atccagatat cttcagattc tggaatctgg
     3361 agctaatgcg aacgaaatag tggctatcac ttttactata aatgctgcaa gtgaaatgaa
     3421 ggagaggata agaaaatata taagagagta cattgataac tttggagaaa ctaaaagcat
     3481 aaattataac tgctttaaag atattgcaga tagtcctatc tctactatcc atggttttgc
     3541 tgcagcgata ataagggaaa acccctatga gtgtaatctg ggctcaagga taaaaatatt
     3601 agaaggactt gatagaaaac tatttgtcca ggagtcggta gatgagtttt taattaacaa
     3661 atcacaaaag gataaaaacg ctacggaaat atttaaaaat gaaaattaca attacgcttc
     3721 agttaaaaac aaattaatag aaatactgga attaactttc agacatcata taaacattta
     3781 tgaccttaaa gccgatgata aggatttaaa agctaagatt gataagcttt acaaaaaact
     3841 atcaagtgaa ataaaactgg ggccttatgg atcagagaat aataaaataa acactaagtt
     3901 aaaagagttt catgccttta taaatgatct tggtaactgc gaacttagaa tagataaaat
     3961 taaaaaactg agtaaagctt gccaattgct ttcaaataca gcaagtccaa aaggaatact
     4021 gcagcttaat aattcaaatg atttagaaag acattttgcc gctaattgcg ctgaaataat
     4081 aaaccagatt attaatctga tggagcttag taatagcaga agctatatgg atctgttttt
     4141 aaaattttat gagttttata aagaaaagaa aagattaatc gggtatttgg agtttgatga
     4201 ccttcttcgt atttgtctaa atcttcttaa aaataatccg gatatattac aggaatatca
     4261 aaacaggttc aggtactttc ttgtagatga atttcaggat acagatgatt tgcagtatga
     4321 gttgattaat ctgttttcaa gctctaatca ttttatcgta ggcgatccca aacagtcaat
     4381 atttggtttt agaggagcaa gccctgagat atttaaaaaa ctgcaaaaaa actctgacaa
     4441 ttcatcatta acctataatt acagatcaga ttacagactc atagcttttt ataatgagct
     4501 gtttaaagta attattaaag aatcctatca gaaaatggaa gtagaatcag agggtagtac
     4561 agcctctgcc agagttaagg agattcttta caccaatgta agtaaagatg agactagggt
     4621 tcttgtagaa tctgcaagca tatctagaaa agttgctgaa cttctggcac aaggttataa
     4681 atgcaaagat atagctatat tgctcaggtc aaaatcacac atatcaagtt ttgaatctgc
     4741 tctttgcgag gcttctatcc catactacag ctcagagcag gcaggatttt acagatattc
     4801 agaaattaga gatatagtct gtatgctaaa atatattaat aacaaaaacg atagaatatc
     4861 cgaggcttct gttcttagat caacttttgc gggggttagt gataccgagc tttttaatta
     4921 ttatgtaaaa gacaagccat cggagttaat cacagattac ctggaatttg tctcacaact
     4981 aagggatgaa tctgctgtcc taagacccgt agaattacta actcgcatat taaataagac
     5041 cagattttgg gaggcaatgc ttgctcttgc tgacggaaag agaaagtatg attcaatcac
     5101 caagctggta gaactattct cacagcttga aaatcaggga aagagcctca ctgaaataat
     5161 acattttctg gacttaaact acgaagaaaa cacagagggt ctatctcagc aggaattgga
     5221 tgaatctgac actgttaaaa ttcttacggt gcataaggca aaggggcttg agtttgctgt
     5281 tgtaatactt gcagatatta atcatggcat gggtggaggc tctgaaacag tgagcttttc
     5341 aaaaaaggat ggagtgatcg taagaaatac aggcataaaa tcaaagttat ggaaagagat
     5401 agaagaggag caaaagaaaa acacatatga agaggaaaaa cgctcgctct atgttgcaat
     5461 cacaagagca aaggaaaaac tgatattgtc ggtatgtcct gactcacgat taaacaaggg
     5521 gtcgtatatg gatatattaa actccgtact gtcactaacc aaaattgaaa aggatgacaa
     5581 aatattaaat tttaaaaatt tcaagattcc cgtagttcga gcatcagagg ccatttatac
     5641 tatccctgtc gttaatgatg atgagaaaga aatcatatat gagcctgagc tacctataat
     5701 atatgaaata gatgtagcgg taaacgacga aactttgaat gaaatagacc cgcccgatct
     5761 gagcataaaa ctaaacaaca tacagattgg ttcagtcatg cacaggtacc tgcagatctg
     5821 gaattattct cagcatagta taaagagcac aataaattat gtactaaacg aatattttat
     5881 ccttgactct aagatggagg agctgttgat ccaattatct gataattatt tgaattcgga
     5941 tttgtttgct cagattaaag aagcagaaga aataaaaagg gagtttccat tttatattga
     6001 acttgatgga aagcatgaaa gaagaaagat tgatctgctg gtttttaaag acgacaatat
     6061 atttctttat gattataagc tctcttcgga ggttaagccc gaacacatag atcagatgga
     6121 tttatatgaa aaagctttaa taaataaata taattgtaat aatatatcta aaaatctggt
     6181 tcatgtacct gatgtgatta ttaaaaatct aaactaggca aagatttatg gaacatatcc
     6241 aaaaatattt agactatttc aataacatag attatgcaca aaaaagtgtt gattttggtg
     6301 ctgaaattgg cttatttatc ggaatctgcg ttctagcgta tatagcaaat tatgtagcaa
     6361 agaattttat attaagagca gttgagggtt taattagaaa gagtaagaca gactgggacg
     6421 acatacttgt agaaagaaag gttttgagaa ggctctctca tattgctccg gcgctgatat
     6481 tttatttcct tgtgcctctg ctatttaagg gaaatgagtc tgtaaaaatc ttaacgcaaa
     6541 ggcttgcatc gatatatatg cttggatgcg gacttcttac tgtagacgcg tgcctctctg
     6601 ctgttcagga tatattcggc aaattccaga tttcaaaaca aatcccgaca agcatattta
     6661 ttcaggttat aaaaattctt ctgtatctgc tggcagctgt aattgtaatt tcaatactaa
     6721 taggtaagtc ccccgcattt ctgatcggaa gcctcggtgc tgttacggcg atcctgatgc
     6781 ttgtgttcaa agatcccata ctcagctttg ctgccggtat tcagctttcc agcaacaaga
     6841 tggtacaggt gggcgactgg atagagatgc caaagcacgg ggtagacggt gatgttattg
     6901 aattatctct tacgactgtg aaagtccaaa attttgataa aactattgtt actgtacctc
     6961 cctatgcact tgtaacagaa tcatttaaaa actggcgggg gatgagtgaa tccggcgtaa
     7021 gaagaattaa aagagcgatt aatgttgata tttcttctgt tactttttgc tcagagcaga
     7081 tgatagaaag atttaagaaa atacaatata tatcaggcta tgtagatgaa aaaaggactg
     7141 aacttacaga atataataaa aaacagaaca tttatgatac tgaccttgct aatggacgga
     7201 gaatgaccaa cattggcact ttcagggcat atattgtttc ttacttaaag gaacacccca
     7261 agatttctaa gaaccatact cttctcgtaa ggcagcttga tcccggccca accggaattc
     7321 caattgagat atatgtattt acgaccgata ataactgggt caactatgag ggaatac
//
LOCUS       DBNGSV010000247         1562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1221743, whole genome shotgun sequence.
ACCESSION   DBNGSV010000247 DBNGSV010000000
VERSION     DBNGSV010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1221743"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1343
                     /locus_tag="AC159U_09150"
                     /pseudo
     CDS             496..1343
                     /locus_tag="AC159U_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1356..>1562
                     /locus_tag="AC159U_09155"
     CDS             1356..>1562
                     /locus_tag="AC159U_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HIR3337261.1"
                     /translation="MEQTALKRQREKPEVADIFRSYGKQYRSTHPVSYEQKKVMGHIQ
                     VCRSAVLGGHIEQCNRCGLERNAYN"
BASE COUNT          456 a          371 c          333 g          402 t
ORIGIN      
        1 tatcaccaca tcttgactca tagttgagca actaaagaaa caaatcaata gggtagagaa
       61 agtcaaaaac agaaaagcat atcggtacat acttctaatt cctccctgct actttactat
      121 gtttgaaaac aaatgttatc aaatgtaata tttaaacgcc atgatagctt ttttgtgaga
      181 atattgccac aaaatttttg agtgtacgtg ataggttgga aagcagtgag gattcttgca
      241 actatcagag atcacgctcg gagggatttt actctaacat taatttggta ttgtgaaatc
      301 tttcggcctt ctaattcttt ttatagatgg tagaactgaa ccgctacgcg gagttattgt
      361 ataagcgtct caccgaagga ttttatcctg cctgtcaatt atcatttctt gcatgaccta
      421 caaatcaatt atactattct tccttacagt aatggttttc ttcatttcat taaccgttta
      481 ccaaggaggt gtagtatgag cagagatccc aaaacccaat tcattaacta catgacgctg
      541 caacgatttg ctctatcaac aaagaagaac tacatagctg cctttgaggg attagcacag
      601 tttcaccatc agtctccaga aacactgacc aacgaacaga tccaggagta cctgcggtac
      661 cttatcgaag accgcaaact ctcctgggga acgtgcaaca actttttttc aggcatcgtc
      721 agtttttacc ggaatgtgtg caagtgggat gagacccgtt tttccattcc cccacggccc
      781 cgcattaaga aactgcccag gattttgagc attgaagaag taaaacgact ctttgcttca
      841 gcggctaact tgaaacaccg ggtattactg aagaccattt acagtgccgg gcttcgcgtc
      901 agtgaagtgg ttcgtttaca accagaccac ctggaaagtg acccctcccg gatgatgatc
      961 agggtagaac agggcaaagg caggaaagac cgctacacca ttctctccgg gaaactgctg
     1021 gtggaaatga gacagtactg gtgcacgtac cgtccgggaa catggttatt ccccggatac
     1081 aaaccagaaa tgcacatgtg tgaaggggcc gctcagaggg ctttttataa ggctaaaaaa
     1141 aagccgacat aaccaaacag tgcggtattc ataccctccg tcactccttt gccacccacc
     1201 tgctctacca aggcactgac ctgtatacca ttaaacgact gctcggtcac tcatccctcc
     1261 aaactacctt agtctacctc cacctcgttc agcagcggat taccgaaatc aaaagccctc
     1321 ttgattatct tgacggcgta taggagggct gccccatgga acaaacagct ttaaagcggc
     1381 aacgggagaa accggaagtg gccgatattt ttcgtagcta cggcaagcag taccggagta
     1441 cccatcctgt atcctatgaa caaaagaagg ttatgggtca tatccaggtg tgccgcagtg
     1501 ccgtacttgg gggtcatatc gagcagtgta accgctgcgg tctggaaagg aatgcctaca
     1561 ac
//
LOCUS       DBNGSV010000248         9357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_945221, whole genome shotgun sequence.
ACCESSION   DBNGSV010000248 DBNGSV010000000
VERSION     DBNGSV010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_945221"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC159U_09160"
     CDS             <1..345
                     /locus_tag="AC159U_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3337262.1"
                     /translation="SLVEKKTDLADKRSVHIIITAKGKKLLKTLLIKRSSFELAKNTD
                     SKINNLVETELKNILANLQKHNSYNTFGVCNTCRHLITEQNGFRCGLTKEVLSSTDTT
                     KICHEHEYSEAV"
     gene            complement(342..773)
                     /locus_tag="AC159U_09165"
     CDS             complement(342..773)
                     /locus_tag="AC159U_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR3337263.1"
                     /translation="MKARLKSWLIIIVSAAAGLYLLYTGGVDLYNSAQLSSKGIAVKG
                     EVKRIERHRSGGSSARFYVTVEFKDQSGNSHTFKNTRPYGYLFAPEKGEPIEIIYLNA
                     DPNIAVINSLWQKYLGPLLSLTVGFLFVWLAKSEIFDKKTD"
     gene            complement(806..1120)
                     /locus_tag="AC159U_09170"
     CDS             complement(806..1120)
                     /locus_tag="AC159U_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337264.1"
                     /translation="MRVLMTVSLPVETANDMVMDGMLGKKIKEIMAEQKPEAAYFTAE
                     DGMRTGLFFLNLKDSSEIPKYAEPWFLSFDAYVDIKPVMSPADLQKADKHIKAAAKKY
                     SI"
     gene            complement(1170..1646)
                     /locus_tag="AC159U_09175"
     CDS             complement(1170..1646)
                     /locus_tag="AC159U_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337265.1"
                     /translation="MFSKLIKNKKDAIYVENYFNRFHDAFLKSAKVVSGNKFVQGAPW
                     EPKRKYSNNESRLLDTGLMFFGKIGMFLELKHYNYNWPNEPSDRTVHIYLKSIADINP
                     ELFIHVGLPIIEINFKNISENKFDVQVKLELSLQDNPPDDITINCCKAEQIYLREA"
     gene            complement(1706..2128)
                     /locus_tag="AC159U_09180"
     CDS             complement(1706..2128)
                     /locus_tag="AC159U_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3337266.1"
                     /translation="MSSIKPRERFSTEVTLNPTLVSEFAKAAGDLNPMHHDPDFAANT
                     RFKKVIASGTQTTALLLGATASYFSGHGLTVGLEFWQRFKKPVFADEKIKVELLIINV
                     MPNEKLAGDIVDLRGRIRGSDGKTAVAAKGKVLVTDKL"
     gene            2143..3609
                     /locus_tag="AC159U_09185"
     CDS             2143..3609
                     /locus_tag="AC159U_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HIR3337267.1"
                     /translation="MKNTLTELNFNNTYYSLPQDFYHRVNPTHFDNPHLVTFNKEVSE
                     MIGLDPAGAEKSDFAEYICGKKEIPGSNPIAMYYTGHQFGVYNKDIGDGRAILLGEVI
                     NPENKKWDLHLKGAGRTKYSRQFDGRAVLRSCIREYLCGEALHYLGIPTTRSLCIIGS
                     DEIVQREIPEMGAMLLRVAQSHVRFGSFEGFFYSDQFENIKILADYLITNHFSQLFDS
                     DNKYYELYKEICRNTGKLIAYWQAYGFTHGVMNTDNMSVLGLTFDYGPFGFVEEFDPE
                     FIPNHSDHHGRYSLRNQKSIAHWNLQKLGKCFIDLITSRQINDGLQMYIDTYRDEYIK
                     LMRRKLGLEIPAEQDWQLVEIILECLYSSCVDYTTFFRNLSDFDEDNIKTENPVSKMF
                     SGTEYGKWAAFYSKRLKEEQSNKSRRKELMDSVNPKYILRNYIAQRAIEKAQSEDYTE
                     INKIYKILQNPFDQQPEFEEYSKPAPEEYRNLSLSCSA"
     gene            complement(3620..6214)
                     /locus_tag="AC159U_09190"
     CDS             complement(3620..6214)
                     /locus_tag="AC159U_09190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3337268.1"
                     /translation="MILAADSSNEQPVTEKYTLDNGLTVILEQNHASPVVAVNVWVKV
                     GSACEVEGEFGLAHVHEHMLFKGTEKRGVGEIARVIESSGGDINAFTSFDETVYYVVI
                     ASRFAETALDVLSDAVTSSTFDPEELSKELEVVLEEIRRGEDSPSRVLSQKMFAEAYK
                     HHTYKRPVIGTSESVKSFTREGILDFYKKWYSPDNMVVVVVGDFNESEIKEKINKTFG
                     KINKTPIPECDIPVEPRQNKINPFVISKDIKEGYFVIAFHGPAANSDDTPVLDVLSNI
                     LGGGESSRLYRNIKEEKGIVNSIYSYSFTPKDPGLFVIGGTLKPENSKEALTEALSEL
                     YKLKNEKVSNDELNKAKVNIESDTIYTKETMQGQAQKLGFYEVETGDYVFEKEYLEKV
                     SNVTADDIVRVANKYFNNDNMTPGFLLPSENVSINVNEISQIAEKVSKMADSKPEQKS
                     SDSVEQVFKTKLDNGITLLVKENHAVPIFAARAVFLGGVRFENEKNNGISNLLSDMFT
                     RGTTKRTAEDIAVEVESLAAEIDGFSGKNSIGVAVESLSKNFDKTIDIFSDVIINPTF
                     PEDEIDRAKREILASINRQGDNPIRTAVNTFLSTLYTKHPYRFDSLGKPEVVEKFTKQ
                     DLQKFYKKYINPENMVISVAGDIDTEKVVASLNKQFSGFKGNKLSLPSLKAEKRAKSI
                     RESTVTQKDKAQAHILLGFLAPDLNDNDQYAFEILNTVLAGQGGRLFTELRDMRSLAY
                     SVTSFYTPGLEPGYFGVYIGTAPQKENEAIDGMKEQLELLLAKGITDDELSRAQQYLV
                     GNFEIGLQQNSSQAARIAFDEIYGQGYKEYQKYPERIMSVTKEDVLRVAKKYIDLKKY
                     TITVLRPE"
     gene            complement(6344..7153)
                     /gene="pssA"
                     /locus_tag="AC159U_09195"
     CDS             complement(6344..7153)
                     /gene="pssA"
                     /locus_tag="AC159U_09195"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3337269.1"
                     /translation="MKKKKTRRQRMMPLLPSIMTTGNLFFGLLSIMTSIQIVSMVSND
                     LITTEEFYRKYWWAAAFIGISAFLDLLDGRIARLISSESDFGISYDSLSDLVSFGVAP
                     AVLIYSWGLINSGKLGLMVVLFYVVCTALRLARFNVQSAGVEKYKFTGLPSPMAAGIV
                     ASPILFFAEIRLDPTEIAMWAYLFVTPFIGMLMVSDVPYWKFPRNIKRPFNALVVISI
                     IIAAIITIPEAMILLIAYTYALSGFFLYLYNLVKKQERVRSQEEKQTTRIL"
     gene            complement(7150..7800)
                     /locus_tag="AC159U_09200"
     CDS             complement(7150..7800)
                     /locus_tag="AC159U_09200"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIR3337270.1"
                     /translation="MEEFRPKIAADGLKFIIPLAVITWLFALWGCVTLSLIFGILTGF
                     IIFFFRDPDPDIPAGSNLILSPAHGKVVSIKNISEPNFLTEEMQCISIFLSIFDCHVN
                     RNPYPGTIKSTKYKPGEFKLAFTDHASDDNERFSMLLETESREPIVVSLITGFLARRI
                     VPLIKMGDKLSIAQRIGLIRFGSRVDIYLPKDVQLMVSLGDKVVGGQSIIGEFKSK"
     gene            complement(7800..8816)
                     /gene="ilvC"
                     /locus_tag="AC159U_09205"
     CDS             complement(7800..8816)
                     /gene="ilvC"
                     /locus_tag="AC159U_09205"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3337271.1"
                     /translation="MKVYYNADINTDILKGKKIAVIGYGSQGHAHALNLKESGMDVIV
                     GLREGGSSWDKAKNAGFDVMNVADATKAADIMMILAPDTSQKDIYDNSMKDNLEAGNA
                     IGFSHGFNIHYKQIVPPDGVDVFMVAPKAPGHTVRNEYTLGGGVPALIAVENNPTGTA
                     KEIGLAYAASIGSGHAGIIETTFKDETETDLFGEQSVLCGGISALITAGFETLTEAGY
                     PPEMAYFECLHEVKLIVDLIYEGGIANMRYSVSDTAKYGDITRGPRVINDSVKQEMKK
                     ILSEIQSGEFANEWIEENKNGRPNYNRLLKEGETHPIEDIGATLRGMMSSLFKRKLVD
                     KTKN"
     gene            complement(8821..9318)
                     /gene="ilvN"
                     /locus_tag="AC159U_09210"
     CDS             complement(8821..9318)
                     /gene="ilvN"
                     /locus_tag="AC159U_09210"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3337272.1"
                     /translation="MRHTISILVDNESGVLSRISGLFSARGFNIESLCVAEAREPGTS
                     RITLVTSGDPHVIAQVVKRFNNMVNVIEVNDLTTQTRVEREMVLVRVDTKKEYRAEIL
                     RIVDIFRAKVVDVGQKNYMLELTGDKDKLTAFIDLLAPMGISEIARTGTVALKRDLQN
                     KQKEK"
BASE COUNT         2784 a         1961 c         1571 g         3041 t
ORIGIN      
        1 tcacttgtag aaaagaaaac tgatcttgca gacaaaaggt ctgtgcacat tattattact
       61 gcaaagggga aaaaattgct aaaaaccctt ttaataaaaa gaagttcttt tgaattagca
      121 aaaaacacag atagcaaaat caataacttg gtagaaactg aacttaaaaa catattagct
      181 aatttgcaaa aacataactc ttataacacc ttcggagtat gcaatacatg tagacatcta
      241 atcacagagc aaaatggatt tagatgtgga ttaactaaag aagtgttgtc atccactgat
      301 acaactaaga tatgccatga gcatgaatac tctgaggcag tctaatcagt ttttttatcg
      361 aatatctctg attttgctaa ccaaacaaat aaaaatccca cagtcaaact aagcaatggt
      421 ccaagatatt tctgccatag ggaatttata acggcaatat tcggatcagc atttaaataa
      481 ataatttcta tgggctcacc tttttcagga gcaaacagat atccataagg cctggtgttt
      541 ttaaatgtat gcgaattacc actttgatct ttaaattcaa ccgtcacata aaaccttgca
      601 gagcttccgc cagacctatg tctttctatt cttttaactt ccccttttac agcaatccct
      661 ttagaggata gctgagcgga gttatataga tcgaccccgc ctgtatatag caagtataga
      721 ccagcggcag ctgaaacaat aattatcagc caagatttga gtctggcctt catcaaaatt
      781 taaaactata aactagctta caatcctaga tggaatactt cttggcagca gcctttatat
      841 gtttatcggc cttttgcaag tcagctggag acataactgg cttaatatct acataggcat
      901 caaatgacaa gaaccatggt tcagcatatt tcggaatctc ggaactatcc ttaagattta
      961 gaaaaaacag acctgttctc attccatctt cagctgtaaa atacgcagct tctggttttt
     1021 gctcggccat tatctctttt attttcttac caagcatccc gtccatcacc atatcgtttg
     1081 ccgtttctac cggcagactt acagtcatta gtactctcaa ttgtttttcc tcctacatat
     1141 tgaactagat ttataataga ttaaatattt taagcctctc ttaaataaat ttgttctgct
     1201 ttacagcaat taattgtaat atcatcgggc ggattatctt gtaatgataa ctctaacttt
     1261 acctgcacat caaatttatt ttcactaata tttttaaaat taatttctat gattggtaaa
     1321 ccaacatgaa taaatagttc agggttaata tcggcaattg attttaaata tatgtgtact
     1381 gttctatctg agggttcatt tggccagtta tagttataat gtttcaattc taaaaacatc
     1441 ccaattttac caaaaaacat tagtccggta tctaataatc tactttcatt gtttgaatat
     1501 tttcttttag gctcccaagg tgctccctgt acaaatttat tacctgatac tacctttgct
     1561 gattttaaaa aggcatcgtg aaatctattg aaatagtttt ctacataaat tgcatccttt
     1621 ttatttttga ttaatttact aaacattacg gtagaactgt aaaaatatta aacgtttatt
     1681 aattttatcc cctaagtaat ctctcttata atttatcagt cacaagcact ttacccttgg
     1741 cagcaactgc tgtctttccg tctgatcccc taatccttcc tctaaggtca acaatatccc
     1801 ctgctagttt ttcattaggc atgacattaa taataagcaa ttctaccttt atcttttcat
     1861 cggcaaatac aggtttttta aacctctgcc agaactcaag cccaactgtc aagccgtgcc
     1921 cggaaaaata ggaagcagtt gcacccaata acagtgctgt agtctgcgta ccacttgcaa
     1981 ttactttttt aaatcgtgta ttggccgcaa aatcaggatc gtgatgcatg gggtttagat
     2041 cacctgcggc tttagcaaac tctgaaacca gggtaggatt aagtgtaact tcagtactga
     2101 atcgctctct tggttttatt gaagacataa caacaattat atatgaaaaa tactcttaca
     2161 gaacttaatt ttaataatac ttattatagt ctgcctcagg atttctatca tcgagttaat
     2221 cccactcatt ttgataatcc acacctggta acatttaata aagaagtctc tgaaatgatt
     2281 ggcctggatc cggcgggagc agagaagtct gactttgctg aatatatatg tggcaaaaaa
     2341 gaaatacccg gctcaaaccc tatagcgatg tactataccg gccatcagtt tggggtttac
     2401 aataaagata taggtgatgg cagggcaata cttttagggg aagttattaa tcctgaaaat
     2461 aagaaatggg atttacatct taaaggcgcg ggtcgcacaa aatactctag acagtttgat
     2521 ggcagggcag tcttgaggtc atgcattagg gaatatctct gcggggaggc attgcattat
     2581 cttggaattc ctactacaag atcgctctgc attataggaa gtgatgagat agtacagagg
     2641 gaaataccgg aaatgggagc catgctcctt cgggtagctc agtctcatgt ccgcttcggt
     2701 tcatttgagg gattttttta cagtgaccaa tttgagaata tcaaaattct tgcagactat
     2761 ttaattacaa accatttctc tcaattattt gattcagata acaaatatta tgagctttat
     2821 aaagaaattt gtagaaatac tggaaagtta atagcttact ggcaggccta tggatttact
     2881 catggagtga tgaatacaga taatatgtct gttctcggac ttacttttga ctacggcccc
     2941 tttggatttg tagaagagtt tgatcccgaa ttcattccaa accattcaga ccatcacggc
     3001 agatattctc ttagaaacca gaaatccatt gcccactgga accttcaaaa gctgggcaaa
     3061 tgttttatag atcttattac ttcaagacaa ataaatgacg gccttcaaat gtacattgac
     3121 acatacagag atgaatatat aaaacttatg agaagaaaat taggtctcga aattccagca
     3181 gaacaggact ggcagcttgt agaaatcata ttggaatgtc tttacagctc ctgcgtagat
     3241 tacacaacct ttttcagaaa tctatccgat tttgatgagg ataatatcaa aactgaaaat
     3301 ccagtttcta aaatgtttag cggcaccgaa tatggtaagt gggcagcctt ttattcaaaa
     3361 agattaaagg aagaacagag caataaatca agaagaaaag agcttatgga ctcagtaaac
     3421 cccaaatata ttcttagaaa ctatatagct caaagagcca tagaaaaagc tcaatcggaa
     3481 gactatacag agattaacaa aatctataaa atattgcaga acccatttga tcaacaaccc
     3541 gagtttgaag aatacagcaa acccgcgcct gaagagtata gaaacttatc ccttagctgt
     3601 tctgcttgat ttagattttt tactcaggcc ttaaaacagt aattgtatac tttttaaggt
     3661 caatatactt cttagctact cttaatacat cctctttggt cacagacata atacgttcgg
     3721 ggtacttttg atattcttta tagccctgcc cataaatttc atcaaacgct atacgtgctg
     3781 cctgagatga gttttgctga aggccgattt caaaattacc taccaggtac tgctgcgccc
     3841 tgctgagctc atcgtctgtt attcctttgg ccagtagtag ttccagctgc tctttcatgc
     3901 cgtcaatggc ttcattttcc ttttgagggg cggtgccgat ataaacccca aaataacccg
     3961 gctctaaccc gggtgtataa aaagatgtaa cagaataagc caggcttctc atatctctaa
     4021 gttcagtgaa gagccttccg ccctgccctg caagtacagt atttaaaatt tcaaatgcat
     4081 actgatcatt atcgtttaaa tcaggtgcta aaaacccaag cagtatatgt gcctgagcct
     4141 tgtctttttg agttacagtc gattctctga ttgattttgc acgtttttct gccttaagtg
     4201 aaggcaggct cagtttattt cccttaaaac ctgaaaactg tttattgagt gatgccacaa
     4261 ctttttcggt atcaatgtct cctgcaactg aaataaccat attctccggg ttaatatatt
     4321 ttttatagaa tttttgcagg tcctgtttag taaacttttc tactacttcg ggttttccaa
     4381 ggctgtcaaa acggtacgga tgttttgtgt aaagtgtgga aagaaaagta tttactgctg
     4441 ttcttattgg attatcaccc tgcctgttga tactggcaag tatctctctt tttgccctgt
     4501 caatctcatc ttcgggaaaa gtagggttaa ttattacatc tgagaatata tcaatagttt
     4561 tatcgaagtt cttactgaga gattctacgg caacaccgat gctgttttta cctgaaaaac
     4621 catcaatttc ggcagccaag gattctactt ctacggctat atcctcagcc gttcttttag
     4681 tagtaccgcg tgtgaacata tcagataaca gattggatat tccattattt ttttcatttt
     4741 caaacctcac tccaccaaga aacacggccc ttgcagcaaa aatagggact gcatgatttt
     4801 cttttacaag aagcgttatt ccattatcga gttttgtttt aaatacctgt tctactgaat
     4861 ccgaagattt ttgctcaggc ttactatctg ccattttgga aactttctca gcaatttggg
     4921 aaatctcatt tacattaatt gaaacgtttt ctgaagggag cagaaaccca ggggtcatgt
     4981 tgtcattatt aaaatattta tttgcaaccc ttacaatatc atcggcagtt acgtttgata
     5041 ccttttccag atactctttt tcaaacacat agtcgcctgt ttcgacctca taaaaaccaa
     5101 gtttttgtgc ctgcccctgc atggtttctt ttgtataaat agtatcactt tcgatgttta
     5161 cctttgcttt attcagttcg tcattactca ccttttcatt tttaagctta tataattcag
     5221 acagcgcctc agttaaagct tctttcgagt tttcaggctt aagagtgccg cctattacga
     5281 aaagacctgg atctttggga gtaaaagagt atgaataaat gctgttaact attccctttt
     5341 cttcttttat gtttctgtat agacgcgagc tttcacctcc gccaagaatg ttcgagagta
     5401 catccagcac aggtgtatca tcactatttg ctgctggtcc atgaaaagcg attacaaaat
     5461 atccttcttt tatatccttt gaaataacaa aaggattaat cttattttgc ctaggttcta
     5521 cgggtatatc acattcagga atcggagttt tattaatttt tccaaaagtc ttattaattt
     5581 tttcttttat ttctgactcg ttaaaatcac ctactactac tacaaccata ttgtctggtg
     5641 aataccattt tttataaaaa tcaaggattc cttctcttgt aaaactcttt acgctctctg
     5701 atgttcctat tacaggtctt ttatatgtat gatgtttata agcctctgca aacatcttct
     5761 ggctaagtac cctgctaggt gaatcctctc cccttcttat ttcttccaaa acaacttcaa
     5821 gctccttact cagctcttca ggatcaaaag tagagcttgt tacagcatct gaaagcacat
     5881 ccagggcagt ctcggcaaac ctgcttgcta tcacaacata ataaacagtt tcatcaaacg
     5941 aggtaaaagc attaatatca ccgccgctgc tctctattac ccttgcaatt tctccaactc
     6001 ctcttttctc ggtaccctta aaaagcatgt gttcgtgtac gtgagcaaga ccaaactcac
     6061 cctcaacttc gcaggcactt cctaccttca cccacacgtt cactgcaact accggtgagg
     6121 catggttttg ttctagtatc actgtaagac cgttatcaag cgtatatttt tcagtaacgg
     6181 gttgctcatt tgagctgtct gctgcaagta tcatatctgt ctccttgaat aaaactaaag
     6241 aaagtaaaat caataaaatt gttcttaatt ttttcattgt atgtaatatt accctaattg
     6301 tattaaagtt tagatgtatt aaacccggga atggattaaa ttgttataat attcttgttg
     6361 tttgcttttc ctcttgtgat ctcaccctct cctgcttctt aaccaggtta tatagataaa
     6421 gaaaaaatcc gctcaaagca taggtatatg ctataaggag tatcatcgcc tcagggatgg
     6481 tgatgatagc cgctattata attgaaatca caacaagtgc attaaaaggc cttttaatgt
     6541 ttcttggaaa cttccagtat ggcacatcac ttaccatgag catcccaata aacggggtta
     6601 caaaaagata agcccacata gcaatttccg tgggatcaag ccttatttct gcaaaaaaca
     6661 gaattggaga agccacgata cccgcggcca tagggcttgg aaggcctgtg aacttgtatt
     6721 tttctacccc tgcagactgc acattaaacc ttgcaagcct gagtgcagtg caaactacat
     6781 aaaaaagaac caccataagt cctaactttc ctgagttaat aaggccccat gaataaatga
     6841 gcacagcagg agctacacca aatgatacaa ggtcgctcag tgaatcatag gatataccaa
     6901 aatcactttc tgaacttatt agtcttgcta tccttccgtc tagcagatca agaaaagccg
     6961 atataccaat aaaagccgct gcccaccagt actttctata aaattcttct gttgtgatca
     7021 gatcattaga taccatgctt actatctgaa tcgaggtcat tattgaaagc agcccgaaaa
     7081 acagatttcc tgtagtcatg atacttggca gtagcggcat catacgctgc cttctggttt
     7141 ttttcttctt catttacttt taaactctcc gatgattgat tgtccaccta ccactttatc
     7201 acccaaactt accattaatt gtacatcctt cggcaggtaa atatcaaccc ttgatccaaa
     7261 tcttatgagt cctatcctct gagcaatgct gagtttatca cccattttaa tcagaggcac
     7321 tattcttctt gcaagaaatc cggttatgag tgaaacaact atcggctccc tactctccgt
     7381 ttcaagaagc atggaaaatc tctcgttatc atccgaggca tgatcggtaa aagcaagttt
     7441 aaactcaccc ggtttatact ttgtagattt tatagttccg gggtatgggt ttctgtttac
     7501 atgacagtca aaaattgaca agaatatgct tatgcactgc atttcttctg tgaggaagtt
     7561 tggttctgaa atatttttaa tagagactac ttttccatga gctggtgaga ggattaaatt
     7621 actgcctgca ggaatatcag gatcagggtc tctgaaaaag aatataataa aacctgtgag
     7681 aatcccgaat atcagagaaa gtgttacaca accccaaagt gcaaacaacc atgtgataac
     7741 tgcaagaggg ataataaact taagcccgtc agcagcaatc tttggtctga attcttccat
     7801 taattttttg ttttatctac aagttttctc ttaaacagag aactcatcat acctctgagt
     7861 gtagcaccaa tgtcttcaat cggatgagtc tcaccctctt ttagaagcct gttgtagttt
     7921 ggccttccat ttttgttttc ttcaatccat tcatttgcaa actcacccga ctggatttca
     7981 gaaaggatct ttttcatttc ctgcttaaca ctgtcattaa ttactctagg gcctcttgtg
     8041 atatcaccat actttgctgt atcacttact gaataacgca tatttgctat accaccttcg
     8101 tagatgaggt ctacgattaa tttaacctca tgaaggcatt caaaatatgc catttctggt
     8161 ggatatccag cctctgtaag ggtttcaaaa ccggcagtaa taagtgcgga tattcctccg
     8221 caaagtacgc tctgctcacc aaaaaggtca gtttctgttt catctttaaa tgtggtttct
     8281 ataatgcctg catgccctga gcctatggaa gcagcgtatg caagtccgat ctctttggca
     8341 gttccagtgg ggttattttc tacagctata agcgcaggaa cacctccacc aagtgtgtat
     8401 tcgtttctta ctgtatgtcc cggtgccttt ggagccacca taaatacatc aactccatct
     8461 ggaggaacaa tctgtttgta atggatatta aaaccatgac taaatccgat tgcatttcct
     8521 gcttccaggt tatctttcat tgagttgtca tatatatctt tttggcttgt atcaggtgca
     8581 agaatcatca taatatcggc tgccttggta gcatcagcta cattcatcac atcaaacccg
     8641 gcatttttgg ccttatccca gctgcttccg ccttctctaa gtcccacaat cacatccatt
     8701 ccgctctctt ttagattaag ggcatgagca tgtccctgac tgccgtaacc aataacagca
     8761 atcttctttc cttttagaat atctgtattt atatctgcat tgtaataaac cttcatctac
     8821 ttatttctcc ttctgtttat tttgtaagtc tcttttaagt gctactgtac ctgttcttgc
     8881 aatttcactt atacccatcg gagcaagaag atcaataaaa gctgtgagct tgtctttatc
     8941 acccgtaagt tcaagcatat agtttttctg accgacatct actacttttg ccctgaatat
     9001 atcaactatt cttagtattt ctgctctgta ttcctttttt gtatcaaccc ttaccagcac
     9061 catttcacgc tcaacccttg tttgtgttgt gaggtcattc acttctatga cgtttaccat
     9121 attattaaag cgctttacta cttgagcaat gacatgcggg tctccacttg taacaagagt
     9181 tatcctggaa gttccgggtt cgcgtgcctc tgccacacaa agactctcaa tgttaaagcc
     9241 ccttgcgcta aacaaacctg aaattctgga aagaaccccg gattcgttat ctactagtat
     9301 agaaatagta tgtctcaaat atagttcctc caatcaaatt agtaataatt aggaaat
//
LOCUS       DBNGSV010000249         3867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_945333, whole genome shotgun sequence.
ACCESSION   DBNGSV010000249 DBNGSV010000000
VERSION     DBNGSV010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_945333"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1639)
                     /gene="cimA"
                     /locus_tag="AC159U_09215"
     CDS             complement(38..1639)
                     /gene="cimA"
                     /locus_tag="AC159U_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR3337273.1"
                     /translation="MSKRVVNIYDVTLRDGTQGEDIAFSINDKIRIAHRLDDLGVHYI
                     EGGWPGSNDRDEGFFEEAKKLKLKHAKFAAFGSTRRAKVTCSKDGNIQALLKAQTPVV
                     TIVGKSWDFHVKEALKISYEENLDLVNDSVKYLKKRVDEVFFDAEHFFDGYKNNPDYT
                     VQVLKAALDAGVDSLVLCDTNGGTLPWEVEEIINEVNEKLDNPPLGIHTHNDSEVGVA
                     NALYAVRAGASQVQGTMNGYGERCGNSNLCSIIPNIQLKLGFKCLQDDLKKLYDTSHF
                     IHELTNLPPIKNRAFVGESAFAHKGGLHVSAVMKNSQTYEHIDPEIVGNHRRVLISDL
                     SGRSNILYKTKELGINIKSDNPKIPNILSNLKTLENQGYEFEGADASFEILVKKSIDK
                     FNKHFDLISARTISEKREEDEDSLAEATVIVKVDGVTEHTAAIGNGPVNALDTAFRKA
                     LEKFYPSLKEVKLLDYRVRVVSTKQGTESVVRVLIESGDKDSRWSTVGVSENIVEASW
                     KALVDSIEYKLMKDDDKKAKKVKRK"
     gene            complement(1636..2871)
                     /locus_tag="AC159U_09220"
     CDS             complement(1636..2871)
                     /locus_tag="AC159U_09220"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3337274.1"
                     /translation="MALIVQKYGGTSVANIDKIRWVAKHIVNTKNKGNKVVAVVSAMA
                     GETDRLVQLALSVMDPPDEREFDVITSSGEQVSSGMVAMMVKSMGEDAVSYQGHQIRI
                     LTNKSYSKAKIVKIDDSSVKKALKKGQIVIVAGFQGVDEENNVTTLGRGGSDLTAVAL
                     ASVLNADSCELYKDDVDGVYTSDPGMVSSAKKLGKISYEEMLEMSSLGSKVLQARAVQ
                     FAKQFKVPLHVKPTANPDAEGTWVLEEKKAMQDMEQTLISGVTYDKNQAKITLAHVPD
                     KPGVAATIFNEIGENEIVVDMIVQNISTEGFTDMTFTVPKTDFKKTLALTKKLVKELG
                     VKKIVSDNKIAKISLVGVGMKTHSGVASKMFTALADEGINIMMISTSEIKISCVIEEK
                     YTELAVRTLHDAFNLGDKK"
     gene            complement(2823..3335)
                     /gene="tsaE"
                     /locus_tag="AC159U_09225"
     CDS             complement(2823..3335)
                     /gene="tsaE"
                     /locus_tag="AC159U_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3337275.1"
                     /translation="MYIENTKDRIILTVDSVRETLQIGEIIGRTAQQGTIIALNGDLG
                     AGKTVLVKGIAKGLEIEEEPNSPTFVILQTYSGKHPLYHFDFYRLSSASELDDLNFED
                     FFYGQGVTVVEWAERISEVFPQYSINIDIEIPDESNNDGPVQSRNITIKGEEKWLLSF
                     RNTVEQALQT"
     gene            complement(3325..>3867)
                     /locus_tag="AC159U_09230"
     CDS             complement(3325..>3867)
                     /locus_tag="AC159U_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3337276.1"
                     /translation="LIKLLKNSTVPVVIDADAITLISNNLRTLKSLIVPVILTPHPGE
                     MARLIGKKNKDVQNDRINVSREFSRKFGVYIVLKGARTVISTPDGKIFINPTGNPGMA
                     AGGMGDVLTGIIGGLLAQGYNAENSCKIGVFVHGLAGDIASEEISESGFLASEIADRV
                     PRALNNIKRNKVRDYIYRVY"
BASE COUNT          946 a          889 c          683 g         1349 t
ORIGIN      
        1 ctgggcaggc attttcgatt ggtttgactg gaactaacta tttcctctta accttcttag
       61 ccttcttgtc gtcatctttc ataagcttat attcgatgct gtctacaagg gctttccagc
      121 ttgcttctac tatattttca gaaactccca cggtactcca tctgctgtct ttatctccgg
      181 attctataag aacacgcact acggattccg taccctgttt ggttgatacc actcttacac
      241 gataatcgag aagctttact tcttttagag aggggtagaa tttttcaagg gcttttctaa
      301 atgcggtatc aagcgcattt acggggccgt ttcctattgc agcggtatgt tctgttactc
      361 cgtctacttt tactattact gtagcctcgg caagagaatc ttcatcttct tctcttttct
      421 ctgagatggt tcttgcgcta atgagatcaa agtgcttgtt gaatttgtcg atggatttct
      481 ttaccagtat ctcaaaagaa gcgtcggcac cttcaaactc ataaccctga ttctcaaggg
      541 ttttaagatt agagagaatg tttggaatct ttggattgtc tgatttaata ttaatcccaa
      601 gctcttttgt tttatatagt atattgcttc tgccagatag atcggagatt agcactcttc
      661 tatggtttcc tacaatctca gggtctatat gttcataggt ctgtgaattt ttcatcacag
      721 cactcacatg caggccgcct ttatgtgcaa aagcgctttc tcctacaaat gccctgttct
      781 ttatgggggg caggttggtg agttcatgaa tgaaatgcga agtatcataa agctttttaa
      841 gatcatcctg caggcattta aatccaagct ttagctgaat gtttggaatt atggagcaaa
      901 gattggaatt gccgcatctc tcgccataac cgttcattgt tccctgcacc tgagaagctc
      961 cggctcttac tgcatacagc gcatttgcta ctccgacttc actgtcatta tgagtgtgaa
     1021 taccaagagg aggattatcc agtttttcat ttacttcatt tattatctct tcgacttccc
     1081 atggaagtgt gcccccgttt gtatcacaaa gcactagaga atccactcct gcatcaagag
     1141 cggcctttaa tacctgcacg gtataatctg ggttattctt atagccgtca aaaaaatgct
     1201 cggcatcaaa aaatacttca tcaactcgtt ttttcaggta ctttacggaa tcattaacca
     1261 gatcgaggtt ttcctcatat gaaattttga gcgcttcttt tacatgaaag tcccagctct
     1321 tacccacaat agtgacaacc ggggtctgtg ctttcaataa cgcctgaata ttcccgtcct
     1381 tgctgcatgt aactttagct cttctggtgc ttccaaaagc tgcaaacttt gcgtgtttta
     1441 gctttaattt cttggcttct tcaaaaaatc cttcatccct gtcatttgag ccaggccagc
     1501 cgccttcgat gtaatgcaca ccaaggtcgt cgagtctgtg tgcaatcctg atcttatcat
     1561 ttatggaaaa ggcaatatcc tctccctgcg ttccgtctct tagtgtaacg tcataaatgt
     1621 ttacaactct tttgctcatt tcttatctcc aaggttaaat gcgtcgtgca gcgttcttac
     1681 ggcaagctca gtatattttt cttctatcac acatgaaatt ttaatctccg aagtgcttat
     1741 catcataatg tttataccct catctgcaag ggctgtaaac attttggatg ctacaccgga
     1801 gtgtgttttc atcccaacac ctacaagtga tattttcgcg attttattat cagatactat
     1861 tttttttacg cctaattctt ttacaagttt ctttgtaagt gccaatgttt tcttaaaatc
     1921 cgttttaggc actgtaaaag tcatgtctgt aaagccttcc gtgcttatgt tctgaacaat
     1981 catatccacc actatctcgt tttctccgat ctcgttaaat attgttgctg caactccggg
     2041 cttatcgggt acatgggcaa gagtgatctt tgcctgattt ttatcgtatg ttactccaga
     2101 aattaaagtc tgttccatat cctgcattgc ttttttttcc tccaaaaccc atgtgccttc
     2161 ggcatcgggg tttgctgttg gttttacatg taagggtacc ttaaactgct ttgcaaactg
     2221 cacggctctt gcctgaagca ctttagatcc aagactcgac atctcaagca tttcttcgta
     2281 tgaaattttg ccaagtttct tagcactgct aaccatcccg ggatcagacg tgtaaacacc
     2341 atcaacgtcg tctttataga gttcacagct gtctgcattt agaacagagg ccaatgccac
     2401 tgccgtaagg tccgaaccgc ctctgccaag ggttgttaca ttgttttctt catccacacc
     2461 ctgaaatccg gcaactatta caatctgacc tttcttaaga gcctttttta cacttgaatc
     2521 gtcaattttt acaatctttg ctttggaata ggatttattg gttaatattc ttatctgatg
     2581 accctgatac gaaacagcat cttcacccat agattttacc atcattgcta ccatgccaga
     2641 cgatacctgc tcaccgcttg atgtaataac atcaaactcc ctttcatccg gagggtccat
     2701 aacggacagg gcaagctgta caagtctgtc ggtttctcct gccatggccg atacaacggc
     2761 tacaacttta ttgcctttat ttttagtgtt tacaatatgc tttgcaaccc atctgatctt
     2821 gtctatgttt gcaacgcttg ttccaccgta tttctgaacg ataagagcca tttttcttcg
     2881 ccttttattg taatgtttct gctttgcaca gggccgtcat tattgctttc atctggtatc
     2941 tcaatatcta tatttattga atattgcgga aaaacctcac ttatacgttc tgcccattct
     3001 actacagtta ccccctggcc ataaaaaaag tcttcaaagt ttaagtcatc cagttcgctt
     3061 gctgatgaca acctgtaaaa gtcaaaatgg tataatgggt gttttccgct gtaggtttgc
     3121 aaaattacaa acgttgggct gtttggttct tcttctattt ctaatccttt agcaatacct
     3181 tttacaagga ctgtttttcc tgctccaaga tcaccattta gagcgattat tgtaccttgc
     3241 tgtgctgtgc gtcctattat ctctcctatt tgtaatgttt ctcttacgct gtctactgtt
     3301 aatattattc tgtcttttgt gttttcaata tacacggtaa atataatctc ttactttatt
     3361 tcttttaatg ttgtttaaag ccctgggtac cctgtcggca atttctgatg ccaaaaaccc
     3421 tgattccgaa atctcttcgc ttgcaatgtc acctgcaagg ccgtgaacaa agactcctat
     3481 tttacatgaa ttttccgcat tgtaaccctg tgccagtaat cccccgataa ttcccgttag
     3541 tacatctccc attcctcctg cagccatacc cgggtttccg gtagggttaa tgaatatctt
     3601 cccatcagga gtggatatga cagttcttgc tccttttagg acaatataaa ccccaaattt
     3661 cctggaaaac tctcttgata cattaatcct gtcgttttgt acgtctttat tcttctttcc
     3721 aataagcctt gccatttctc cgggatgtgg ggttaaaatc accggcacaa tgaggctttt
     3781 tagtgtacgt aaattattag aaataagtgt tatagcgtct gcatcaatga ctacaggtac
     3841 tgtagaattt ttaagaagct taataag
//
LOCUS       DBNGSV010000250         1027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1775807, whole genome shotgun sequence.
ACCESSION   DBNGSV010000250 DBNGSV010000000
VERSION     DBNGSV010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1775807"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1022
                     /locus_tag="AC159U_09235"
     CDS             63..1022
                     /locus_tag="AC159U_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3337277.1"
                     /translation="MKYHEIVVVKRWIPILKEYERTKAKVTPRPFKFVKDLCEAHHIH
                     KKELRRYYLKWIEGGRKEESLFPQKRGPRPGSRRTPKEIERNIIKAYRRFGSNRYELV
                     LMFKPYYLDKTPSPATMDRIKNRYPLNESEKKVIKRYEKKAPGELAHIDLSSVPSDIT
                     HKLKIKNLYIAALCDDCTRLVYIEIVRDKKASTMTYFMARSLSWFKQIYGFEFERIMS
                     DNGAEFKGKLEYEHPFEMMCKELGVKHTYTRIGRPQTNGKIEAFFKIAKKEFFSPNKF
                     DSMEDLILNLGNYLFEYNHLRRHGGLNYETPFDKLQKVTELLS"
BASE COUNT          386 a          152 c          220 g          269 t
ORIGIN      
        1 ttgtaaatgc tcagtaatta agtaaccaaa ttagtatata ttaataacta aggaggtgct
       61 taatgaaata ccatgagata gtggtagtaa agagatggat accaatactc aaagaatacg
      121 agagaactaa agccaaagta actcccaggc cctttaagtt tgtaaaagac ctctgtgaag
      181 cccatcatat acacaaaaaa gaactaagaa gatattacct aaaatggatt gagggtggaa
      241 ggaaagaaga gtctttgttt ccacaaaaaa gaggtccgag accagggagc cgtagaaccc
      301 ctaaagagat agagagaaat ataataaagg catacagacg gtttggctca aaccgttatg
      361 agcttgtgtt gatgttcaaa ccttattatc tggataaaac tccttctcct gccacgatgg
      421 atcgtatcaa aaacagatac ccgcttaatg agagcgagaa gaaagtaata aaacgttatg
      481 agaagaaagc ccctggggag cttgcccata ttgatttaag cagtgttcca agtgatataa
      541 cacataagct caaaataaag aatctttaca tagcggctct atgtgatgac tgtaccagac
      601 ttgtttatat agagattgta agggataaaa aggcatcaac tatgacatat tttatggcaa
      661 ggtcactgtc gtggtttaag cagatttatg gctttgagtt tgaaagaatt atgtcggata
      721 acggggcaga gtttaagggt aagcttgagt atgaacatcc gtttgaaatg atgtgtaagg
      781 agcttggagt aaaacataca tatacaagaa taggaagacc acagacaaac gggaagatag
      841 aagcattttt taagatagcg aaaaaagagt ttttcagtcc aaacaagttt gattcaatgg
      901 aggatttaat attaaatctg ggtaattatt tatttgaata taatcatctg agaagacatg
      961 gaggtttgaa ctatgaaacc ccttttgata aactacaaaa agttaccgaa ttactgagct
     1021 agtacaa
//
LOCUS       DBNGSV010000251        13547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_529763, whole genome shotgun sequence.
ACCESSION   DBNGSV010000251 DBNGSV010000000
VERSION     DBNGSV010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_529763"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..957
                     /locus_tag="AC159U_09240"
     CDS             235..957
                     /locus_tag="AC159U_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR3337278.1"
                     /translation="MKTDHDEQELDLSEKGRSKDGTVISLDKRLFLQFLAFGDCDDAT
                     SIARHLSSSGTQGALYLDINDPFGIGLLTLSEDPDYFVTGLREVLNTDPFMSLYIKEE
                     YTMLGRTYSFGYEPDLEATLLTGPRKKVLDPEWKWAIWYPLQRKKEFERLSEDEQRAI
                     LGEHGKLGFKFGKAGYAKDIRLACHGIDKNDNDFVIGVIGKELHPLSACIEAMRKTKQ
                     TSQYLESLGPFFVGKAIWQSEI"
     gene            complement(975..1280)
                     /locus_tag="AC159U_09245"
     CDS             complement(975..1280)
                     /locus_tag="AC159U_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3337279.1"
                     /translation="MAKSLTKSQLASELSEKTGITKKSATAVLDELADIAYREAENSF
                     TLPGVGKLVLIQRKARKGRNPATGEEIDIPAKKDVKFRVSKVCKDSILSKYKMFGLS"
     gene            complement(1433..1804)
                     /gene="arsC"
                     /locus_tag="AC159U_09250"
     CDS             complement(1433..1804)
                     /gene="arsC"
                     /locus_tag="AC159U_09250"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780654.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR3337280.1"
                     /translation="MSKKVSKLNNIILHNPRCTKSRQTLKLTEDNNLDLSIIEYLKTP
                     PTVSELDEICNKLGVEPLEITRTKEKLFAELGLTAKDKKTRKEWLKILSENPKLIERP
                     IVIYNNKAIIGRPPEKVLEIL"
     gene            1883..3949
                     /locus_tag="AC159U_09255"
     CDS             1883..3949
                     /locus_tag="AC159U_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR3337281.1"
                     /translation="MKSYFSFIILLLFLISSQVAECDEAVDKSQILLDPIEVTSTRIN
                     KNINKVPFSLSLIDKDELQLGEQTITLDESLNKIPGVYVQNRYNFAQDQRISIRGFGS
                     RAAFGVRGIKILYDGIPLTLADGQSQTDSIDLSSAERIEVIRGPVSALYGNASGGVIS
                     IFSETGFEQPFFDVKSVFGSFGLYRLNAKAGGQVENLSYLINLSRLNYDGFRDHSKTE
                     NVQLNSNFNYLLGVNSDITVVLNYIDAPRADDPGALTKEQINQDRNQASAGNLVSNSG
                     ESIEQLRIGLKFNSYLNDENSIETLTYIITRDFEQRLPFGIVSFDRIFLGGGFKYINT
                     SKFLNHNNRLILGTDIKYQDDDRVNFSFTDEFRKDALTLDQTEQVFNIGLYMQNEFSI
                     LDNLEFSLGFRYDYYNFSVDDFFINDNNPDQSGSRSFNQINPKIGILYSPLEYINIYG
                     NFSTSFETPTTTEFINNPEGSGGLNPDLDPQKTIGFEIGVKGVIENKLNCEIALFYAD
                     IDDQLIPFEVEEFPGRDFFRNAGSSRQYGIEILLGYSLTKNIRTSFSYTYLNFEFTDF
                     EAEDEIFDGNDVPGIPDHQIHADILYKSDMGLYAGADFLYVSDFFVNDSNSEENESYK
                     VLNLKTGFERSFYNKLLINAFLGLNNVLDEKYNSSVRVNAFGDRFFEPAPEFNLFGGL
                     SLKYTI"
     gene            4034..4300
                     /gene="purS"
                     /locus_tag="AC159U_09260"
     CDS             4034..4300
                     /gene="purS"
                     /locus_tag="AC159U_09260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR3337282.1"
                     /translation="MKEYKVKVEVKLKPVVLDPQGKAVLSSLHNLGFSEVEDCRVGKL
                     IELNIKDSDSENIKDRVDDMCKKLLSNPVIEDYKISMEEILKDG"
     gene            4293..4991
                     /gene="purQ"
                     /locus_tag="AC159U_09265"
     CDS             4293..4991
                     /gene="purQ"
                     /locus_tag="AC159U_09265"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR3337283.1"
                     /translation="MAKFAVIVFPGSNCDHDCHHALKKVLDQQSKLLWHEETDLDDFN
                     CVVIPGGFSYGDYLRTGAIARFSPVIESVKKFANEGKPVIGICNGFQILVESGLLPGG
                     FIRNSSLKFVCKWVNLTVENTDTPFTNLCEKGDVLKIPVANGEGNYFASEADLKQLQD
                     NSQIVLRYSDKNGKVDSNSNPNGSTYNIAGICNKQGNVLGMMPHPERCCEQVLGGEDG
                     KVIFESILNFINNN"
     gene            5086..5544
                     /locus_tag="AC159U_09270"
     CDS             5086..5544
                     /locus_tag="AC159U_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3337284.1"
                     /translation="MTDDIQGLRRILKECKTIAMVGLSQKWNRPSNYAAKYLLLHGYE
                     VIPVTPTYEEVLGQQCYGSILDIPKKVDVVDCFRKEEEIPAIARDAVKIGAKVLWMQL
                     GIVNEQARQIAEDGGLEVVMNRCMKIEHGRLFGGISFLGVNTSVITSKLQ"
     gene            5578..6867
                     /locus_tag="AC159U_09275"
     CDS             5578..6867
                     /locus_tag="AC159U_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR3337285.1"
                     /translation="MADREFGFETKCLHAGQKPDPATNSRATPIYQTTSYVFNSPEHA
                     ASLFNLQEFGNIYTRIMNPTTAVFEERMAALEGGTAALVVASGMASQMAAILTILEHG
                     DEIVSSSTLYGGTYTQLDFSFRKMGINTTFVNPDDPENFRKAITKKTKAIYSETIGNP
                     LGNVLDIQAVSDIAHEAGIPHIIDNTFATPYLCNPFEHGADIITHSATKFIGGHGTSI
                     GGVLIEAGTFPWDNGNFPTMTEPSAGYHGVKFYETFGSIGYAIKARCEVLRALGPAPS
                     PFNSFLFLQGLETLPFRMERHCANALEVANHLNDHPFVEWVKFSGFPDSPYYDLGKKY
                     LPKGIGSIFTFGIKGGIDAGVKFIESLELLSHLANVGDAKTLIIHPASTTHRQLNEEE
                     QASAGVTPEMVRISVGLETVDDIIWDIDQALEKSQKK"
     gene            6864..8030
                     /gene="metX"
                     /locus_tag="AC159U_09280"
     CDS             6864..8030
                     /gene="metX"
                     /locus_tag="AC159U_09280"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetX"
                     /protein_id="HIR3337286.1"
                     /translation="MTTDELGESPVAIVNEGSVGIVEPQSFTFGYPPDELELENGSKL
                     GPITVAYETYGSLNKNRDNVILIEHALTANAHAAGKHSPGDQTPGWWDSMIGPGKAFD
                     SDKYYIICSNVLGGCYGTTGPSSINPKTNKPYGYDFPIITIRDMVNVQKKLLHHLGIA
                     SIACIAGGSMGGMQAIEWSLLYPDMVKSIILVASGACSNPQAIAIHKVGIQAIKDDPN
                     FSGGDYYDKESPDKGLAIARMIGHITYLNDKMLWEKFGRRSDDIKDMKSSFKYRFEIE
                     KYLQYQGKKFVSRFDANSYIYIMRAIDMYDASEGYDSLDESFSRLNIRRAIVISFTSD
                     WLAPSYQSKEIVRALEANNIDTTYKEIKSQYGHDSFLLKYKQLTPPIRKLFEEL"
     gene            8027..8809
                     /locus_tag="AC159U_09285"
     CDS             8027..8809
                     /locus_tag="AC159U_09285"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3337287.1"
                     /translation="MRLGIALGGGGAKGYAHIGVLKAFSDMGIEFDVISGTSVGALVG
                     AVYASGNLDRLEEIATKFSVLDIPLLLSPTISKQGFFSGKKIETLLNELISEKNIEDL
                     PKPFSAVCVDLNKPEIVTLSKGNLINAVRASISIPAIFTPIKSGKQLLVDGGVLEPVP
                     VEATRNMGADIVIAVDLLSKSKNFKDLQDGTILDIIQKTSIATQQGLTKFRMQLHPPD
                     LIIEPDVSALGILDFKNGKKVIKIGKEETKKMIPHINKLLGR"
     gene            complement(8798..10189)
                     /locus_tag="AC159U_09290"
     CDS             complement(8798..10189)
                     /locus_tag="AC159U_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337288.1"
                     /translation="MYFIIAGLILIGAVVGINLISSDFDKQINIIDQPVIEFVSLYIV
                     TGLVYFIYIYRIKNFRPKIKNVIIWIFIIGIIARLVMILSVPILEVDFYRYMWDGAVT
                     VSGSNPYKYSPKNLLNDNNNASKELKELALKNENILKRINHPELKTSYPLVTQAVFAI
                     TNLIKPFSLSAWKFVLLFIDIIIFILIYILLKKLKLPESNLIIYWWNPILIKEVFNSG
                     HMDVLVFPFLLGSMLLYLSNRYLFSFTTLTAAVGIKLWPVLLFPVFIRKFISDWKKLL
                     QYFLIFSVLSILILLPLFISYFDTGSGIEAYSRSWQNNESFFRIVLAISEFILPLIGY
                     HPGHGQSVSRIIVGILAIILVLYLIRNKIKNELDYFRIGLAIIAITFFISPTQFPWYY
                     IWMLPFLTFIPYKPLLLLTVLMPLYYLRYYYEPRGEYDFYYQYIVWLEYLPALVWLGY
                     DLISKKFMNRSTA"
     gene            complement(10251..10763)
                     /locus_tag="AC159U_09295"
     CDS             complement(10251..10763)
                     /locus_tag="AC159U_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR3337289.1"
                     /translation="MSGDKIIIEKGDITDFEGDAIVNAANTELILGSGVAGAIREKGG
                     FLIQAECNKTGSILLGEAAITHAGDLNTKYVIHAAGMHLGGQVETEALKSCTLNSLKL
                     ADKFKIKTLAFPAIGTGVGGYPLDKCAEVMLGVVNEYLKSHKTQIEKIYFILFDDNAF
                     NSFNNYIQQI"
     gene            complement(10753..11187)
                     /locus_tag="AC159U_09300"
     CDS             complement(10753..11187)
                     /locus_tag="AC159U_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009638621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3337290.1"
                     /translation="MSWFQKSISLSPKPRGFHIVTKEILEQTPELSKIKVGLAHIFIC
                     HTSASLTLNENVSPDVLTDFESSFNNLVKENEPYYTHTIEGSDDMPAHIKSSLLGNSL
                     TLPITNGRFNLGTWQGIYICEHRNRGGSRKLIVTVNGKTDEW"
     gene            complement(11224..11829)
                     /locus_tag="AC159U_09305"
     CDS             complement(11224..11829)
                     /locus_tag="AC159U_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337291.1"
                     /translation="MFTSFDLISIFYCFIAIGVIRRLIKKRKALIDDDIHIFDKRLIS
                     EVSFFILIPIGVLLHELGHAIAVWYYGGTVVDFEWRVFWGYVSHQGSFTDVQRWWISF
                     SGNLVSILIGIIPLFYISKVRRLIVKEFLITFAKIEIIYSLIFYPVFSFIGFGGDWVR
                     IYDFSIKPYAQITIILHILLMFILWKFGVFKNEESNKPEQF"
     gene            complement(11943..12341)
                     /gene="atpC"
                     /locus_tag="AC159U_09310"
     CDS             complement(11943..12341)
                     /gene="atpC"
                     /locus_tag="AC159U_09310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR3337292.1"
                     /translation="MADKINLEIITPVKVVLNTEVAEVAAPGTLGEFGVLPGHVPFIT
                     TLNPGLVKYTEGSDEKSLFISGGLFNLDGDNAKIITESAELPSDIDSSEASQQVEDIQ
                     KLIEDFQGSKKELAELNKKLKIAEAKVEAS"
     gene            complement(12354..>13547)
                     /gene="atpD"
                     /locus_tag="AC159U_09315"
     CDS             complement(12354..>13547)
                     /gene="atpD"
                     /locus_tag="AC159U_09315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3337293.1"
                     /translation="DALNTGDKITVPVGKEALGRMMNVVGEPIDEAGPIATAERWPIH
                     RPAPSFAEQSTKAEMFETGIKVIDLLAPFLKGGKIGLFGGAGVGKTVLLMELINNVGK
                     EHGGVSVFAGVGERTREGNDLWMEMKESGVIGNTGLVFGQMNEPPGARARVALTGLTL
                     SEYFRDTEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLASDVGALQERIT
                     STTKGSITSVQAIYVPADDLTDPAPATTFAHLDGTIVLSRQLTELGIYPAVDPLDSTS
                     RILDPNLLGEEHYTVAREVQRILQRYKQLQEIIAILGMDELSEEDKMIVARARKVQRF
                     LSQPFSVAEQFTGTPGKYVPIKDSVQGFKELIDGNVDDLPEQAFYMVGNLDEVKEKAK
                     SLVSD"
BASE COUNT         4416 a         2489 c         2543 g         4099 t
ORIGIN      
        1 attccaagac cgaatcttct ggatctcctc tgaaataccg cgtcataaaa attatagtca
       61 acagctacat ctaatccctc tggatatttt cccattgtaa tagtccctcg tagtaatagt
      121 tttgaaaact tagtgtatca aattagaaac tttaaaacaa ttagtttatt aaatgcttta
      181 aaaaattatt gatcatgtaa tatatatcaa catgtttata aggtggattt atatatgaaa
      241 acagatcatg atgaacagga attagattta tctgaaaaag gcaggtcaaa ggatggaact
      301 gtaatatctc tcgataagag actgtttttg cagtttttag catttggtga ctgtgatgat
      361 gcaacaagca ttgctagaca tttaagcagt tccggtacac agggagctct ctatctcgat
      421 attaatgatc cttttggaat tggtctatta acactaagcg aagaccccga ttactttgta
      481 acaggcttaa gagaggtttt aaataccgac ccatttatga gtctatatat aaaagaagag
      541 tatacaatgc tcggaagaac atactcattt ggttatgaac cagacttaga agcaactctt
      601 ctcacaggac ctagaaagaa agtattagac cctgagtgga aatgggctat ttggtacccg
      661 ctgcaaagaa agaaggaatt tgagaggcta tcagaagacg agcaaagagc tatacttggt
      721 gaacatggaa agctgggatt taaatttggt aaagcaggct atgctaagga tatacgcctt
      781 gcgtgtcacg gtatagacaa aaacgataat gattttgtaa ttggagtaat aggtaaagaa
      841 ctccatcctc tatctgcatg catagaggct atgagaaaaa caaaacagac ttctcaatac
      901 ttagaaagcc tgggcccgtt ttttgtcggt aaagctatct ggcaaagcga gatttaactt
      961 tttagaaccc gttcttaaga aagcccaaac atcttatact ttgataagat actgtctttg
     1021 cagacttttg agaccctgaa ttttacatcc ttttttgcag ggatatcaat ttcctcacct
     1081 gttgcagggt ttctgccttt tcttgctttc ctttgaatta acaccagttt acccacccca
     1141 ggtaaagtga aggaattttc agcttctcta taagctatat ctgccagttc atctaataca
     1201 gcggttgccg attttttggt gattcctgtt ttttctgaca gttcagatgc gagttgggat
     1261 tttgtaagtg acttagccat gttgtttaac cctccatcca attcataaat ttagtattac
     1321 tctactacat ttttaataga attgtaatct caaatttaag aaagttccag tgagaataga
     1381 aaattttata aaatgtgttt tgatattcaa ttaatcagac aaccgctaat ttctataaaa
     1441 tttccaaaac tttttcaggt ggcctgccaa taattgcttt attattataa ataactattg
     1501 gtctttctat aagtttagga ttttctgaaa gtatttttag ccattctttt ctagttttct
     1561 tatctttagc tgtcagtccc agctcagcaa acagcttttc cttggttctt gtaatttcaa
     1621 gaggctcaac tccaagctta ttacaaatct catcaagctc tgaaacggtt ggaggagttt
     1681 taagatactc tattatacta agatccaaat tattatcttc tgtaagctta agtgtttgtc
     1741 tgctctttgt acatcttgga ttatgtaata ttatattatt taatttgctt actttttttg
     1801 acataaatat attgtccaat caattaaatt ttattttcaa tacaatatca ataaatctta
     1861 ttactagcaa aatattttac atatgaaatc ctattttagc tttattattt tattgttatt
     1921 tttgatttcg tctcaagttg ctgagtgtga tgaggctgta gataaatcgc aaatattact
     1981 tgatcccatc gaagtaacct caacccggat taataaaaat atcaacaagg ttccattttc
     2041 tttgagctta attgataaag atgaactcca gcttggtgag cagactatca cgctggatga
     2101 gtctctaaac aaaattcccg gagtttatgt acagaacaga tacaacttcg cccaggatca
     2161 gagaatttct ataagaggct ttggctcaag ggcggctttt ggggttagag gtataaaaat
     2221 cctgtacgac ggtattccgc ttacgcttgc agatgggcag agccagacag acagcataga
     2281 tttgagttca gccgagagaa ttgaggtaat ccgagggccc gtttctgcac tttacggaaa
     2341 tgcttcaggc ggagtaatta gtatattctc tgaaacagga ttcgagcagc cattttttga
     2401 tgtcaaatca gtattcggat catttgggct atacaggcta aatgcaaaag caggtggaca
     2461 agtagaaaat ttaagttatc tgataaatct aagcagactt aattatgacg ggtttagaga
     2521 tcacagcaag acagaaaatg ttcaattaaa cagtaatttc aattatctac taggagtcaa
     2581 ttcggatatc acagttgttt taaactatat agatgctcca agggctgatg atcccggagc
     2641 tttaactaaa gaacagatta atcaggatag gaatcaggca tcagctggca atttagtctc
     2701 aaactcagga gaatcaatag aacaactcag gattggctta aaatttaatt catatttaaa
     2761 tgacgaaaat agtatagaga ctctaaccta tattatcacc cgtgattttg agcagaggct
     2821 gccctttgga attgtttctt ttgacagaat ctttttagga ggaggcttta aatatataaa
     2881 taccagtaaa tttctaaacc ataacaaccg tcttatattg ggcacggaca ttaaatatca
     2941 ggatgatgat agggtaaatt tctcatttac tgatgaattt agaaaagatg ctttaaccct
     3001 tgatcagaca gaacaggttt ttaacatcgg gctatatatg caaaacgagt tttcaatatt
     3061 ggataatctc gaattctcat taggctttag atatgactac tacaattttt ctgttgatga
     3121 tttttttatt aatgacaata atcctgatca gtcaggctca agatcgttta accagataaa
     3181 tccaaaaata ggaatacttt attctccctt agaatatata aatatatacg ggaatttctc
     3241 aacttccttt gagacaccta caactacaga gtttataaat aaccccgaag gctcgggcgg
     3301 gcttaatcct gatctcgatc cacaaaaaac aattggcttt gaaattggtg taaagggggt
     3361 tattgagaat aagctcaatt gtgaaattgc gcttttttat gcagatattg atgatcagct
     3421 tatcccattc gaagtagaag agtttccagg aagggatttt ttcagaaatg caggtagttc
     3481 aagacaatat gggattgaaa tattgttagg atattcccta acaaaaaaca tccgtacctc
     3541 tttttcttac acttacttaa attttgaatt tacagacttc gaagctgaag atgaaatctt
     3601 cgatggtaac gatgtaccgg gaattcctga tcatcaaatt catgcagata tattatataa
     3661 atcggatatg ggcttgtatg caggagcaga ttttctgtat gtttccgatt tttttgttaa
     3721 tgattctaat tcagaagaaa atgaaagcta taaagtctta aatctaaaaa cagggtttga
     3781 aagatcattc tataacaaac tcctaataaa tgcctttttg gggttgaata atgtattgga
     3841 tgaaaaatat aactcatctg taagggtaaa tgcatttgga gacagatttt ttgaaccggc
     3901 acctgagttc aatttatttg gcggattatc acttaaatac acaatttaaa aataaaataa
     3961 tgagttacat cttgaacaac cgggagcaag atggataatt acactcctat tccacttgta
     4021 aattagagaa aacatgaaag agtataaagt aaaagtcgaa gtaaaactaa aaccggtagt
     4081 tttggaccct cagggcaagg cagtcctatc ctcactccac aatctgggat tcagtgaagt
     4141 ggaagactgc agggttggca aattaatcga gttaaatata aaagacagtg attctgagaa
     4201 cattaaagac cgcgtagacg atatgtgcaa aaaactacta tctaaccctg taattgaaga
     4261 ctacaaaatc tcaatggaag aaatattaaa agatggctaa atttgccgta atcgtatttc
     4321 ccggttctaa ctgtgatcac gactgccatc acgcccttaa aaaagttctt gatcagcaat
     4381 ctaagttgct ctggcacgaa gagactgatt tagatgattt taattgtgtg gttataccgg
     4441 gtggtttttc gtatggagat tatcttagga caggagcaat agccagattt tcaccagtaa
     4501 tagaatctgt taaaaaattt gctaatgaag gaaaaccggt gattggtatc tgcaatggtt
     4561 ttcagatttt agttgaatct ggcctacttc ccggaggatt tataagaaat tcctcgctaa
     4621 agttcgtttg taagtgggta aacttaaccg tcgaaaatac cgacactcca tttacaaacc
     4681 tttgtgaaaa gggggatgta ctaaaaatcc cggttgcaaa tggagaagga aactattttg
     4741 catctgaagc tgacctgaaa caactccaag ataattccca gatagtactt cgatactccg
     4801 ataagaatgg aaaagtagat tcaaattcca accccaatgg ttcgacatac aacattgcag
     4861 gcatttgcaa caaacaagga aatgtactgg gtatgatgcc ccacccggaa aggtgctgcg
     4921 agcaggtgct aggtggcgag gatggaaaag taatatttga atcaattctc aacttcataa
     4981 acaacaacta atcctaaaat tttgtagtca catttatttt tatagccgta tttccaaaat
     5041 gagcatataa tttcaggaat taataattct aaggtaattt gatagatgac cgacgatata
     5101 caaggactta gaagaatatt aaaagagtgt aaaaccatag caatggtggg tctttcacag
     5161 aaatggaata ggccaagcaa ttatgcagca aaatatctgc ttttacacgg atatgaggtc
     5221 atacccgtaa cacctacata cgaagaggtg cttggacaac aatgttatgg tagcatattg
     5281 gacataccta aaaaggttga cgttgtggac tgttttagaa aagaggaaga aatccctgca
     5341 attgcaagag atgcagtcaa aatcggagcc aaagtgctgt ggatgcagct tggaattgta
     5401 aatgaacaag caagacaaat tgcagaggac ggcggacttg aagtagtaat gaacagatgt
     5461 atgaaaatag aacatggaag gctttttgga ggtataagtt ttttaggtgt gaatacctca
     5521 gtgataactt cgaaattaca ataaaaatac aaatagaata tttacaagga gaagaaaatg
     5581 gcagatagag aatttggttt tgaaacaaag tgtctacatg ctggacaaaa gcccgacccg
     5641 gcaacaaact caagagcaac acctatatat caaaccacct cctatgtgtt taacagtccc
     5701 gagcatgcag caagcctgtt taacctccag gagtttggaa atatatatac aagaataatg
     5761 aaccccacta cagcggtatt tgaagaaagg atggcagcgc ttgagggtgg tacggcagca
     5821 cttgttgtag catccggaat ggcctcacaa atggcagcaa ttctaactat tctggaacat
     5881 ggagatgaga ttgtttcatc aagcactctt tacggaggca catataccca gcttgacttc
     5941 agctttagga aaatgggtat aaacacaact tttgtaaatc cagatgatcc ggaaaatttc
     6001 agaaaagcca taacaaaaaa gactaaggcc atttattcag agacaatagg aaacccactg
     6061 ggtaacgtgc tggatataca ggctgtatca gatatagctc atgaagccgg cattccacat
     6121 attattgata acacatttgc aactccatat ctctgtaatc catttgagca tggtgcagat
     6181 ataatcacac attcagccac aaaattcata ggtggtcatg gtacttcaat tggtggagtt
     6241 ctcatagaag caggtacatt cccttgggat aacggtaatt tcccaactat gacagagcct
     6301 tcagcaggct atcacggcgt aaagttttat gaaacattcg gtagtatagg ttacgctata
     6361 aaagcaagat gtgaggtact aagggcactt gggcctgcac caagcccctt taactcattt
     6421 ttgttccttc agggtcttga gacactacca ttcaggatgg aaaggcactg tgcaaatgca
     6481 ctggaagttg ctaatcacct gaatgatcac ccatttgtag aatgggtaaa attttcagga
     6541 tttcctgaca gcccatacta tgatctgggt aaaaaatatt tacctaaagg aattggctca
     6601 atttttacat tcggtattaa aggtggtatc gatgcagggg taaaattcat agaatcactt
     6661 gaattactaa gccaccttgc aaatgtaggt gatgcaaaaa cgctcattat tcacccggca
     6721 tccactactc acaggcagct taatgaagaa gaacaggcat cggcaggtgt taccccagaa
     6781 atggtaagaa tttccgttgg ccttgaaaca gtagatgata ttatctggga catcgatcag
     6841 gcgcttgaaa aatctcagaa aaaatgacga cagatgagct gggtgaatca cccgttgcga
     6901 tcgtaaatga aggttctgtg gggattgtcg aacctcaatc ttttacattc ggctatcccc
     6961 ctgatgaatt agagctggaa aacggctcaa agctggggcc tattacggtt gcttacgaaa
     7021 cttacggcag ccttaacaaa aacagggata acgtaatatt aatagaacac gctctcacgg
     7081 caaatgccca tgctgccgga aaacattcac ctggtgatca aactccgggt tggtgggact
     7141 ccatgatagg ccctgggaag gcatttgaca gcgataagta ctatattatt tgctctaatg
     7201 tgctaggagg gtgttacggt acaactgggc catcttcaat aaatcctaag acaaacaagc
     7261 cttatggtta tgattttccc attataacca taagagatat ggtgaatgta caaaaaaagc
     7321 tactgcatca cttgggcatt gcttccattg catgtatagc aggtggatcg atgggaggga
     7381 tgcaggccat agaatggtct ctcttatacc ctgatatggt taaatcaata attcttgtgg
     7441 cctcgggagc atgttctaac ccgcaggcta ttgcaattca taaggttgga atccaggcaa
     7501 taaaggatga tccaaacttc agtggcggag attattatga taaagagagt cctgataaag
     7561 ggcttgccat tgcaaggatg ataggccaca taacgtatct caacgataag atgttatggg
     7621 aaaagtttgg cagacgatct gatgatataa aagatatgaa atccagtttc aaatacaggt
     7681 ttgagataga aaaatatctt cagtatcagg ggaaaaaatt tgtgagcagg tttgatgcca
     7741 acagctatat ttatattatg agggccatcg acatgtatga tgcatctgag gggtatgaca
     7801 gtctggacga gtcgtttagc agattaaata tacgtagagc tatcgtaata tcatttacat
     7861 ccgactggct tgctccctct tatcagtcaa aagaaattgt tagagcgctt gaggctaaca
     7921 atatcgatac gacttataag gaaataaaat ctcagtacgg acacgactca tttttgctca
     7981 aatacaaaca actcacaccg cctataagga aactcttcga agaactatga gactcggaat
     8041 agcccttgga ggcgggggtg caaagggata tgcccatatc ggtgtcttaa aagcatttag
     8101 tgatatggga atagagtttg atgtaatctc gggaacaagt gtcggggcac tggtaggagc
     8161 tgtctatgct tcaggcaacc tcgacaggct tgaagaaatt gcaaccaaat tcagtgtatt
     8221 agatattcca ttactgctct ctccaacaat ttctaaacag gggtttttca gcggaaaaaa
     8281 gatagagact cttttgaatg agttaatctc tgaaaaaaat attgaggatc ttccaaaacc
     8341 attttctgca gtatgtgtgg atttgaataa accagagata gttactctta gtaaagggaa
     8401 tttgataaat gcagtgaggg cgagcatttc aataccagca atttttaccc caataaaatc
     8461 aggaaagcag cttctagttg acggaggagt attagaacct gttcccgttg aagcaacaag
     8521 aaatatgggt gcggatatag taattgcagt tgatcttctt tcaaaatcta agaatttcaa
     8581 ggacttacag gatggaacca tactggatat tattcaaaag acttccatag ccactcagca
     8641 gggacttacc aagttcagaa tgcagcttca tccgcctgat ctaataatag agccggatgt
     8701 gtcagcactt ggtattctgg attttaaaaa tggaaagaaa gtgattaaga taggcaaaga
     8761 ggaaactaaa aaaatgatcc ctcatattaa taaattatta ggccgttgat ctattcataa
     8821 attttttact aatcaaatca tatccaagcc atacaagtgc aggcagatat tcaagccaaa
     8881 caatatactg gtaataaaaa tcatattcac ctcttggttc ataataatat ctcagataat
     8941 aaagtggcat tagcactgtt agcagcagaa gtggtttgta tgggataaaa gttaaaaatg
     9001 gcagcatcca tatgtaatac cacggaaact gtgttgggct tataaaaaaa gtaattgcta
     9061 taatagctaa accaattctg aaataatcaa gctcgttctt tattttattt ctgattagat
     9121 agagaactaa aatgattgcc aaaattccaa ctattattct agaaacggac tgtccatgac
     9181 ccggatgata tccaattaag ggtaaaataa attcagatat cgccagaact atcctaaaaa
     9241 aagactcatt attctgccag cttctactat aggcttctat tcccgaacct gtgtcgaagt
     9301 atgatataaa aaggggtaaa agtatcagga tggataatac tgaaaatatt aaaaagtatt
     9361 gcaataattt cttccagtca ctaataaact ttcttataaa tacagggaat agcagaacag
     9421 gccagagctt aatacctaca gcagccgtta aagtagtaaa tgagaataag tacctgttcg
     9481 ataaatacag aagcattgag ccaagaagaa aaggaaagac caacacgtcc atgtgccccg
     9541 agttaaacac ttcttttata agtattgggt tccaccaata aataattaaa tttgattcag
     9601 ggagctttaa tttttttaga agaatataga tcaggataaa tatgattata tctatgaaaa
     9661 gcaatacaaa tttccatgcg gatagactga atggttttat aagattagtt atcgcaaata
     9721 cagcttgagt aactagggga taagatgttt tgagctcagg atggtttatt cgttttaata
     9781 tattttcatt tttaagagct aattctttta attctttaga ggcattattg ttatcattaa
     9841 gtagattttt aggtgaatat ttataaggat tacttccgct tactgttaca gctccatccc
     9901 acatgtacct gtaaaaatca acctctaata ttggtacaga taggatcatc acaagtcgtg
     9961 caatgatccc aataataaaa atccagataa tcacattttt aattttgggt ctaaagttct
    10021 taattctgta gatgtaaata aaataaacaa gtccggttac aatatataaa gaaacaaatt
    10081 ctataactgg ctggtctata atatttattt gtttgtcaaa gtcagatgaa attaggttta
    10141 taccaacgac tgcacctatt aatatcaatc ctgcaataat aaaatacata tattatttat
    10201 acacaactat gtggattcta atattagata gattttacaa caaagtggaa ttatatttgt
    10261 tgaatataat tgttaaatga attaaatgca ttatcgtcaa acagaataaa gtatattttt
    10321 tctatttgtg ttttatgtga ctttagatac tcgtttacaa ctcctaacat aacctctgca
    10381 catttatcaa gcggatatcc accaacccct gttcctattg ctggaaatgc cagtgttttt
    10441 attttgaact tatctgctaa tttaaggctg tttaatgtac aactttttag cgcctcagtt
    10501 tcaacctgtc ctccaagatg catacctgct gcatgtataa catattttgt atttaaatca
    10561 cctgcatgag tgattgcagc ctcacctaat agaattgaac ctgttttatt gcattcagcc
    10621 tgtataagaa agccaccctt ttcccttata gcacctgcaa caccgcttcc aagtataagc
    10681 tccgtatttg cagcatttac tatcgcatcc ccctcaaaat ctgttatatc acctttctct
    10741 attattattt tatcaccact catctgtctt cccgtttacc gtaactatca attttcgaga
    10801 accacccctg tttctgtgtt cacaaatata aataccctgc catgtgccca ggttaaacct
    10861 gccgttagtg ataggtaacg ttagactatt tcccagaaga gatgatttta tatgggccgg
    10921 catatcatca gatccttcga tagtatgcgt gtaataaggc tcattttctt taaccaaatt
    10981 attaaaactg ctttcaaaat ctgtcagcac atcaggggat acattttcat tgagcgtaag
    11041 agaagcagat gtatgacata taaatatatg tgctagtcca accttgattt tggaaagttc
    11101 cggtgtttgt tcaaggattt cctttgtaac aatatgaaaa cctctgggct ttggagatag
    11161 agaaattgat ttttgaaacc agctcataat tagtcctttt taaattttta ggctctagta
    11221 taactagaat tgttctggtt tattagactc ctcattctta aatacaccga acttccataa
    11281 tataaacatc aataatatat gtaatataat agttatttgc gcataaggct ttattgaaaa
    11341 atcataaatc cttacccagt cacctccgaa gcctataaag gaaaagacag ggtaaaatat
    11401 gagtgaatat attatttcaa ttttagcaaa agttattaga aattctttta caataagtct
    11461 tcgtacttta cttatataaa aaagtgggat tattccaata agaattgaga caaggttacc
    11521 actaaatgaa atccaccagc gttgaacgtc tgtaaaactg ccctgatgac ttacatatcc
    11581 ccaaaatact ctccattcaa aatcaacaac agttcccccg tagtaccata cagcaatagc
    11641 atggcccaac tcatgcagca gtactcctat aggtattaga ataaaaaagg aaacctctga
    11701 tattaacctt ttatcaaaaa tatgaatatc atcatctatt agtgctttcc gcttttttat
    11761 taaacgtcta attacaccta tagctataaa gcaataaaat atggaaatta gatcgaatga
    11821 tgtgaacata tcttagagat attaccctat tgataaggta atttatgtat aaaataaaaa
    11881 acggattgat attattcaat aatatcaatc cgcaaattat ttttatttta agtaacttct
    11941 atttaagaag cctctacctt ggcttctgca atcttaagtt ttttgtttag ttcagctaat
    12001 tcttttttgc tgccttgaaa atcctcaata agtttctgaa tgtcttcgac ctgctggctt
    12061 gcttctgagg agtcaatatc tgatggaagt tcggcacttt cagtaataat tttagcgtta
    12121 tctccatcta ggttaaataa accgccggag atgaaaagtg acttttcatc gctgccctca
    12181 gtgtatttaa ccaacccggg gtttagagtc gtgataaaag gcacatgccc gggaagtacc
    12241 ccaaactctc caagggtccc cggtgctgca acctctgcaa cttctgtgtt taataccacc
    12301 tttacaggtg tgataatttc taaattaatt ttatctgcca tctgttaata tccttaatcc
    12361 gatacaagtg atttagcttt ttctttaact tcatcaagat ttccaaccat gtagaatgcc
    12421 tgctcaggaa ggtcgtccac attaccatcg ataagctctt taaatccctg tacgctgtct
    12481 ttaatcggca catatttacc cggggttcct gtaaactgct cagcaacact aaatggctgt
    12541 gataagaacc tttgcacctt tcttgcacgg gcaacgatca tcttatcttc ttcagaaagc
    12601 tcgtccatac ctaaaatagc gatgatctcc tgaagctgtt tgtatctctg aagtattctc
    12661 tgtacctcac gagcgactgt gtagtgctcc tctccaagaa ggtttggatc taaaattctt
    12721 gaagttgaat ccagtggatc aacagcaggg taaattccaa gctctgttaa ctgtctagaa
    12781 agcacgatag taccgtccag gtgagcaaat gtagtagcag gagcagggtc tgtaaggtca
    12841 tcagcaggca cataaatagc ctgtactgat gtaatagaac cctttgttgt tgatgtaatt
    12901 ctttcctgca gcgcaccaac gtcacttgca agcgtaggct gataacccac agctgatgga
    12961 atacgtccaa gtagcgctga tacctctgag cctgcctgag taaacctgaa tatgttatca
    13021 ataaataaaa gtacgtcctg accttcagtg tctctgaagt attctgaaag cgtaagacca
    13081 gtaagagcaa cccttgctct tgcaccaggc ggctcattca tctgcccgaa cactagcccc
    13141 gtgtttccta taaccccgga ttctttcatc tccatccata ggtcattacc ctcacgagtt
    13201 ctttcaccaa caccggcaaa cacagataca ccaccgtgtt ctttaccaac gttgtttata
    13261 agttccatta aaagtactgt tttaccaact ccggctcctc caaagagtcc aatctttcca
    13321 ccctttagaa acggtgcaag aaggtcaata accttaatac ctgtttcaaa catctcggct
    13381 tttgtgctct gctcggcaaa gctaggcgct ggtctgtgaa taggccatct ctcggctgta
    13441 gcaattgggc cggcttcatc gattggctca ccaaccacgt tcatcatcct gccaagtgct
    13501 tctttaccaa caggtactgt gattttatca cctgtgttaa gagcgtc
//
LOCUS       DBNGSV010000252         9464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_252258, whole genome shotgun sequence.
ACCESSION   DBNGSV010000252 DBNGSV010000000
VERSION     DBNGSV010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_252258"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..826
                     /gene="tmk"
                     /locus_tag="AC159U_09320"
     CDS             212..826
                     /gene="tmk"
                     /locus_tag="AC159U_09320"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3337294.1"
                     /translation="MFITFEGIEGSGKSTQARLLNDFITTKGLKINLTREPGWGKLGQ
                     IIRNILLDDRDITLAPFAELCLFCTDRVNHVKEFIEPKLENGEIVICDRYHDSTLVYQ
                     GLGRDNDRELVAKMVEQSCLGILPDITLLLDLPVESGLQRIESRDEKTKFDAESVDFH
                     KRVRDGFLESYKKQPDRIKIIDADRDAAVIAQEINTIIENILNI"
     gene            835..1848
                     /gene="holB"
                     /locus_tag="AC159U_09325"
     CDS             835..1848
                     /gene="holB"
                     /locus_tag="AC159U_09325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR3337295.1"
                     /translation="MGFPSITGHEFQKEFLMKSIKKQRVANSYLFYGPEGVGKKLVAL
                     EFAKLLNCERKIDGQGVYDEGKCTCSSCLKIDGSNHPDVTVLKSVDSSEIKVNQIREQ
                     IEDLIYLKPYEGRYKISIIDDAESMNINAQNAFLKTLEEPPADAVIILVCSKPQYLLK
                     TITSRCQSLEFFRLDESVIIQAIKSKSQISAEHYETAARLSQGSLSKALMMDEELLLE
                     RKSFIDNIANIQNRSAVGILELVEDISKEKSSDSNDYNVFFETLFMWLKDILYMKSNI
                     DDKHIVHRDITEATKRFAQIWSIDYILELRSFLEKARESLFFRNVNKQFVLENVIIKF
                     SKT"
     gene            1871..3400
                     /gene="metG"
                     /locus_tag="AC159U_09330"
     CDS             1871..3400
                     /gene="metG"
                     /locus_tag="AC159U_09330"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3337296.1"
                     /translation="MSKSFYITTPIYYVNDVPHIGHAYTTIAADVMARYKKLCGQKVY
                     FLTGTDEHGQKIERTAKEAGKTPKELADEVVVRFADLWKVLNISNDDFIRTTEKRHKI
                     AVNEIFKVIQDNGDIYLGEYEGWYDVRDEAYITDTQYEDFMELPESKRPIIERVKEKS
                     YFFKLSEYQDKLLDYYKNNPDFIKPDNRRNEVISFVEGGLRDLSISRTTFSWGIPIDS
                     DKEHVIYVWFDALTNYITAVGYPNNTEMLNEYWPADYHFVGKDILRFHAVYWPAFLMS
                     AGLELPKTVFAHGWWTIEGEKMSKSLGNVIDPYKIAEEFGVDVFRYFLLREIPFGQDG
                     DFSRDSVVGRINGDLANGLGNLVSRSLGMVEKYFDGVVPSANETETIDKSVADKALQT
                     VDQYNNHMDDVAFSKALFSIWELIAFVNKYIDDTAPWTLAKGGQTQRLSTILYNCCES
                     IRIISILVYPFMPATATDIWEKLGNDSAIEKAGVEGLKEWGIIKQGSKVIKGSNLFNR
                     I"
     gene            3650..3853
                     /locus_tag="AC159U_09335"
     CDS             3650..3853
                     /locus_tag="AC159U_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3337297.1"
                     /translation="MVKINNTSGFTLLEILVAVFIMSVGFLALSQMEFLSVRQKQMAE
                     AGSNATNIIQAAADKDLAEIKRI"
     gene            4145..4396
                     /locus_tag="AC159U_09340"
     CDS             4145..4396
                     /locus_tag="AC159U_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337298.1"
                     /translation="MYLLRKASLSTAVNPLSLKTVTSVVLTYAVKSPKQFADNNFSLS
                     TKHNLAVQGLIVTAQTEDFSDTIPISSGAWGGVLVPNTP"
     gene            4422..4703
                     /locus_tag="AC159U_09345"
     CDS             4422..4703
                     /locus_tag="AC159U_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR3337299.1"
                     /translation="MKNSYGYTIIELLVAVAILSIVFTALVTILMHQQRQFNLTSQGV
                     DVDQTGRIALDFIATEIRNSGARQGKTVAFDFVNGGSGSIHFVTLIQLM"
     gene            4871..5674
                     /locus_tag="AC159U_09350"
     CDS             4871..5674
                     /locus_tag="AC159U_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337300.1"
                     /translation="MIESGDLIGFWSRGSLCNPADPNICLTTPEQCTECGVILKMNSL
                     NTSTRRATISGVNSILSQNFQIADFTDFSVFANNFFIPRIAGVPSEMSIVKSRTFAIR
                     NDNALVMSENGSPTFQAIAGGELDDDTTVGDDVQVGAGIVDLQFVFNLQDADGGITRV
                     GMQPFDDDEAIRQFADFTSISSDTSLSYNGDMRGRQMDTRTVEIYLVVRSRLKPQLIS
                     GNRIPSQTIEALGDVESRPTSHSSLGEGFIYKVFNTVVYLRNLGREEFG"
     gene            5710..6342
                     /locus_tag="AC159U_09355"
     CDS             5710..6342
                     /locus_tag="AC159U_09355"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HIR3337301.1"
                     /translation="MRIEEKTNINLSDESGIALIIALVMLLVMSVMAISVSFLSNTDF
                     QTMTNFKRGQEAFLAAETCVNETRRQIAQEGLAFLLFKQASQKSTDPLIEPLDIDIAD
                     SNSNLIKDSDGIGAKCRSGSRLMDSTVVASIDPIFEFPEDAKSIARDLRGFSKDAASP
                     SNPQATPIVFTVIGKDAKDRDKNDEEIQVNTGTQLATGVEAFLLGGTNVY"
     gene            6332..>9464
                     /locus_tag="AC159U_09360"
     CDS             6332..>9464
                     /locus_tag="AC159U_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337302.1"
                     /translation="MFTKFSKNKEKLFILILGFLFLTALSILLYVDNTKAQSLPRPPI
                     NENEVEDLNLFRGTTIGGNSNLLLYIDYSQSMGTNPAGVQTGNWDQSYTVDDDTVSNC
                     DGFASDGDNSVCIAGTCALRQKIDNVSSGDPEGFAIAHCAANAAGITTELVDKDGGVI
                     HNAYLLNSGDTITITSTDLRPDTTIMRTTDGIVERFNSGIAFEGVFGGCGSRACNRSK
                     FGTCEDPVDFGEFINCIDIAYSDVLCPTCPTTSDETIVISVLANAADVNCGDSTTVST
                     ILGSGLGDTDIVTSLKSTLNTGCMTDRERIWAAAAMDNYSNFLWAETKTMPSDYKTCG
                     AQHCITLQGSGFGTEGSPNLINQVNPSGTLEDHSCNNNTALGIGSGAMEVTTHEVDRF
                     IACMETATQKQPFVKSCTSGGTDGDFCSPGQNGSTRADAMMTVLAQVLDRDNSIKNST
                     CTDTPRLFNGTTSPISCFNYLNTPFRDLGDIPDITGGGSMPVVTGDAKIGDQLDSADE
                     ALAPFRVNIGNYAGIDECDDNNSFNTDQGGGAGMSNEKFNNVFGELGNRRPSGRTPLA
                     SAIGLDDTNPENTGTKNDVLGIYRVTLNSDKENAACKAHFSVFITDGEDNCSGDCNSN
                     QAFGTTGTGMDMDAGGDSSSGTCDNTLAPATGNSNRRSTLQAASFSRTHFARKPVQGN
                     EFNTEVMSFFIGWGVKDNPQAVRTLNAAALLGGTHTTGLLLHRHPQGFISGQKDLDGS
                     PGVPAVLPKADITEIRSSFPWVLEMAEMEHSGGDFSNNPLGVTLSGCQTPNETGSCSW
                     NGTDVFDNDFFTGTAGTDLPTAFTRTNVGQSFAFFVDNPAELIAALEAIFDFTKGFSA
                     VGQAPQVPPSVTAIGLRDRVLLPSFTPTIGEPIWQGRLALFGFLDDPDNPGTKIIVRK
                     PRSDGEFLDGNSLDPEALRSANIFTDTGALSSNAEEFFWDAGKLLAERDIVSSATSRN
                     LLTVGDSTSFGTLQIDNTGSGEFDAIIYTSGIVAFSRALDPEVFGISDLDVDPAGVES
                     VISICSDIAADASS"
BASE COUNT         2970 a         1616 c         2085 g         2793 t
ORIGIN      
        1 gtaatccaaa aatcagaata aaaatgataa taattgctat atgttttctt ggttgcatag
       61 atattccttt gcgaaatttt atattttata actgcataag tataatattt taacttataa
      121 ctccaaattt tgttttaaaa ataaaaacaa atgctaaaat tacaaaatat tcgttgttat
      181 ttatctctct ttaagttact ttctttgctc aatgtttatc acttttgaag gaatcgaggg
      241 cagtggaaaa agcactcagg caaggctgct taacgatttt attacaacta aagggctaaa
      301 aataaatcta acaagggaac ctggttgggg caagcttggg cagattataa gaaatatttt
      361 gctggatgac agggatatta cccttgcccc atttgctgag ctgtgtcttt tctgtaccga
      421 cagggttaat catgtaaagg aattcattga gccaaagctt gaaaatggcg agattgtgat
      481 atgtgaccgt tatcacgact caacccttgt ttatcagggt cttggcagag ataatgacag
      541 ggagttggtt gctaaaatgg tagagcagtc atgccttggc atactgccgg acatcacgct
      601 gctcttggat cttccagttg aatccggact gcagaggatt gagtcaagag atgaaaaaac
      661 aaagtttgac gctgaatctg tagattttca taaaagggta agagacgggt ttttagaatc
      721 ttataaaaag cagccggatc gaataaagat tattgatgct gacagagatg ctgcagtaat
      781 tgctcaggaa ataaatacaa ttatagaaaa tattttgaat atataggaat tgatatgggt
      841 tttcccagta taacagggca cgaatttcaa aaggaatttt tgatgaaatc tataaagaag
      901 caaagagttg caaactctta tctcttttat ggacctgaag gtgttggtaa aaaactagtt
      961 gcgcttgaat ttgcaaagct tcttaactgt gagagaaaaa tagacggaca gggggtttat
     1021 gacgaaggta aatgcacctg ttcatcatgc ctcaaaatag acgggtctaa tcatccggac
     1081 gtaacagtgc taaaatctgt ggattcatct gaaataaaag taaatcaaat acgcgagcaa
     1141 attgaagatt taatttatct aaaaccttat gagggcaggt ataaaatatc tatcatagac
     1201 gatgcggagt ctatgaatat taatgctcaa aatgcatttt taaagactct tgaagaaccg
     1261 cctgctgatg ctgttattat ccttgtttgc tcaaaacctc agtatctgct aaaaaccatt
     1321 acatcaaggt gccagagcct ggagtttttc aggcttgatg aaagtgtgat aatacaagca
     1381 ataaaatcta aatctcaaat atctgctgaa cattacgaaa ccgctgccag actctctcag
     1441 ggcagccttt caaaggccct tatgatggac gaagaacttc ttttagaaag aaagtctttt
     1501 atcgataata ttgctaatat tcaaaataga tcagcagtgg gaatactgga acttgtagaa
     1561 gatatttcta aagaaaaatc atcggacagc aatgattata atgtattttt tgaaactctt
     1621 tttatgtggt taaaagatat actatacatg aaatcaaata ttgatgataa gcacatagtg
     1681 cacagggata taactgaagc taccaagaga tttgcgcaaa tctggtcaat tgactatata
     1741 ctggaattaa ggtcatttct cgaaaaagca agggaatcac tattttttag aaacgttaac
     1801 aaacagtttg ttctggaaaa tgtgatcata aaattttcta aaacatgaac tattggatgg
     1861 tacataacaa ttgagtaaat cattttatat aacaacacct atatattacg taaatgatgt
     1921 cccgcatatt ggacatgctt acacaacaat cgcggctgat gtcatggctc ggtacaagaa
     1981 actgtgcggg cagaaagtat attttctaac cggtaccgat gagcacgggc aaaagataga
     2041 aagaactgca aaggaagctg gaaagacccc taaagagctt gccgatgaag ttgtagtaag
     2101 gtttgctgat ctgtggaagg tgttaaatat atctaacgac gatttcatca gaaccacgga
     2161 gaaaagacat aaaattgccg ttaatgaaat attcaaggtt atacaggata atggagatat
     2221 ctatctggga gagtatgagg gctggtatga cgtaagggac gaagcctata taaccgatac
     2281 ccagtatgaa gattttatgg aattgcccga gtctaaaaga cctatcatcg aaagagtaaa
     2341 ggagaagagc tatttcttta agctttcaga gtatcaggac aaactgcttg attactataa
     2401 aaataaccct gactttataa aacccgataa cagaagaaat gaggtgataa gttttgttga
     2461 aggagggctc agggacctta gcataagcag gacaaccttc tcatggggaa ttcccataga
     2521 tagtgacaaa gaacatgtga tttatgtgtg gtttgatgca cttacaaact atattacggc
     2581 tgttgggtat ccgaataata ccgaaatgtt aaatgaatac tggcctgcgg attatcattt
     2641 tgtaggcaag gacattctga ggtttcatgc agtttactgg cctgcatttc taatgtctgc
     2701 agggcttgaa ttaccaaaga ccgtatttgc acatggatgg tggaccatcg agggagaaaa
     2761 gatgtccaag tcacttggca atgtgattga tccttacaaa atagcagagg aattcggtgt
     2821 ggatgtgttc agatacttcc tgctaagaga gataccattt ggacaggatg gcgatttttc
     2881 cagagattca gttgtcggga gaataaacgg agaccttgca aacgggcttg gaaacctcgt
     2941 gagcagaagc ctcggaatgg tagaaaaata ttttgacggc gttgttccat ctgcaaatga
     3001 gacagagacc attgataaaa gtgtagctga taaagcattg cagactgtag atcagtacaa
     3061 taatcatatg gatgatgttg catttagtaa ggctcttttt tctatctggg aattaatagc
     3121 atttgtgaat aagtatatag atgataccgc tccatggact ttagctaaag gcggtcagac
     3181 gcaaagactt tctactatac tttacaactg ctgtgaatct ataagaataa tatctatcct
     3241 cgtctaccct tttatgcctg ccactgccac cgatatttgg gaaaaattag gcaatgattc
     3301 agctatagaa aaagcaggtg tagagggatt aaaagagtgg ggaattataa agcagggttc
     3361 aaaagttata aaaggctcaa acctgtttaa caggatataa aactttaatc tataagatct
     3421 tatctctttc agtttatgtt tccagaccaa ggtatgctgt gaaatatttt gcgcaattag
     3481 tgaaattttg ttcatagtaa caaattctat tgatatttta actttaaaaa gtataatgtt
     3541 tgcaggttgt tacaatataa acattaagtg gcatatagat tgcaatacaa tatgacagtt
     3601 aatactttgg gatttaagat aattgttagt tatttaacag ggatatagga tggttaaaat
     3661 aaataatact agtggtttca ctctgttaga aatactggtt gcggtattta taatgtcggt
     3721 tggctttctt gccctttcac agatggagtt tttgtcagta agacaaaagc aaatggcgga
     3781 agcaggatct aatgccacta acataattca ggccgctgca gacaaagatt tagccgaaat
     3841 caaaaggata taattattaa acaatcgggc atttctcact gaaaaagatg gaagggctgc
     3901 agactttaca tactgtgatg gttcggaaga ttattctgca tgttccaatt gtccgtgcaa
     3961 tcctcttgcc agtgttacac cggattttac cattacaggc agtggtccta ctattaaaga
     4021 caccgaatgt actgccattg ccctcaataa ttcggatata aaggaattcg aatatactac
     4081 agaccaaact acctgtaaaa gtttttatga tggtcttggg gtcggcagtc ttggtgccga
     4141 gggcatgtat ttgttaagaa aagcttcttt aagtactgct gtaaatccat tatctttaaa
     4201 aactgttact tcagtagttc ttacatatgc tgtaaaatcg cctaagcaat ttgctgacaa
     4261 caattttagc ttgtctacaa agcataatct ggcggttcag gggctcatag ttactgctca
     4321 aacagaagat ttttctgata ctattccaat atcatcggga gcttggggag gggtattagt
     4381 gcctaacacg ccttagtcaa aaaaatatgt atattaaaaa tatgaaaaat agttatggat
     4441 acacaatcat agagctgcta gttgctgtag cgattttgtc tatcgttttt actgcactcg
     4501 tgacaattct tatgcatcaa caaagacagt tcaatctaac ctctcagggg gtagatgtag
     4561 atcaaactgg caggattgcg cttgatttta ttgctacaga gattagaaac tctggcgcac
     4621 gtcagggaaa aacagttgct tttgattttg ttaacggagg cagtggttct atccattttg
     4681 tgacgctaat acaactgatg tagggcagga tgcccctcct gattgtctta cattatatac
     4741 ctgggatatt actgcgggct tcaaaaacga tgaaattcaa tcaacagtat tatccggtaa
     4801 aggaactcta gttggaagca atatcgtagt aaatgttgat ggctggttcc cccccgtttc
     4861 tgatcctgct gtgatcgaaa gcggagatct tataggtttt tggtcaagag gaagtttatg
     4921 caatccagcg gaccctaata tttgtctcac tacacccgaa cagtgtactg agtgtggtgt
     4981 gattttaaaa atgaacagcc tcaatacgtc cacaaggcgc gcaacgataa gcggtgtaaa
     5041 ttcaatttta tcacaaaact ttcaaatagc tgactttact gatttctcag tatttgccaa
     5101 taactttttc ataccaagga ttgccggtgt tccatctgaa atgtccatag taaaatcaag
     5161 aacatttgca ataagaaatg ataacgctct tgttatgagc gaaaatggct cacctacatt
     5221 tcaggcaata gcaggtggag agttggatga tgatacaact gttggagatg atgttcaggt
     5281 cggtgctgga attgttgatc tgcagtttgt ctttaatctt caagatgctg atggtggtat
     5341 tacaagagta ggtatgcaac cttttgatga tgatgaagcc ataaggcaat ttgctgattt
     5401 tacgtcaata agcagtgata cgagtttatc atacaatggg gatatgcgag gcaggcaaat
     5461 ggatacaaga actgtcgaga tatatctggt tgttcgatcc aggctcaaac cccagcttat
     5521 tagcggaaac aggataccct cacagaccat cgaagctctt ggtgatgttg aaagcaggcc
     5581 tacgtctcat tcaagtttgg gtgagggatt tatatacaag gtttttaata cggttgttta
     5641 tttaagaaat ttgggaagag aagaatttgg ataattccag aatataaatt ttaagtaaag
     5701 gaagattgta tgagaattga agaaaaaaca aatataaacc tatccgatga aagtggtata
     5761 gcccttatta tagctcttgt aatgctactt gttatgtctg ttatggcaat ttcggtatcg
     5821 tttttgtcaa atactgattt tcagacaatg accaatttta aaagggggca ggaagctttt
     5881 cttgcggcag aaacatgtgt aaatgaaacg aggcgccaga ttgcacagga aggacttgct
     5941 ttcttgcttt tcaagcaggc ttctcaaaaa tctacagacc cgttgataga gcctctggat
     6001 atagatattg cagatagcaa cagcaacctt ataaaagatt ctgacggtat tggagcaaaa
     6061 tgcagatcgg gaagcaggct tatggacagt actgttgtag cgagtataga tccgatattt
     6121 gaatttcccg aggatgcaaa atctattgca agagatctta gaggattcag taaagacgcg
     6181 gcttcaccat ctaatcctca ggcaacaccc attgttttca ctgtgatagg taaggatgct
     6241 aaggataggg acaagaatga tgaagaaatt caggtaaata ccggtacgca gcttgcaacc
     6301 ggcgtagaag cttttttgct aggaggaacc aatgtttact aagtttagca agaataaaga
     6361 aaaattattt attttaattt taggattttt atttcttacg gcattatcca ttttgctata
     6421 tgtagacaat acaaaggctc agtctcttcc aagacccccc ataaatgaaa atgaagttga
     6481 ggaccttaat ctgtttcgag gcactactat cggcggtaat tcgaatctct tgctatatat
     6541 tgattattct caatcaatgg gtactaaccc agccggtgtg cagaccggca actgggatca
     6601 gtcttatact gtagatgatg atactgtttc caattgtgat ggttttgctt cagacggtga
     6661 taacagcgtt tgtattgccg gcacctgtgc tttgaggcag aaaattgaca atgtttctag
     6721 tggagatcct gaaggatttg caattgccca ctgtgctgct aatgctgccg gaataacaac
     6781 tgaattagta gataaagacg gtggtgttat tcacaatgct tatcttctta acagcggcga
     6841 tactataacc ataacgtcta ctgatttaag accggatact actattatga gaacaactga
     6901 tgggattgtg gaacgattta acagcggcat tgcatttgag ggagtgtttg gtggctgtgg
     6961 ttcaagggca tgtaatagat caaaatttgg tacatgtgaa gaccctgttg attttgggga
     7021 atttataaac tgtatagata ttgcatattc tgatgttttg tgtcctactt gcccaacaac
     7081 ttctgatgaa acaatagtaa tatctgtttt agctaatgcc gctgatgtaa attgcggaga
     7141 cagcacgaca gttagtacaa ttcttggttc aggacttggt gacacggaca tagtaacttc
     7201 tttaaaaagt acattgaata ctggctgtat gactgacaga gaaagaatct gggcagcggc
     7261 agcaatggat aactattcta actttctttg ggcagaaaca aaaactatgc ctagcgatta
     7321 taaaacatgc ggtgctcagc actgtataac tttgcagggc agcggttttg gtacagaagg
     7381 gagccctaac cttattaacc aggtaaaccc atcgggcacg cttgaagatc attcctgtaa
     7441 taacaatacc gcacttggaa taggatcggg tgcaatggaa gtaacaacac atgaggttga
     7501 caggtttatt gcctgcatgg aaacagctac acaaaagcag ccatttgtaa aatcctgtac
     7561 ctccggtgga acagatggtg atttttgctc acctggtcaa aatggtagta ccagggcaga
     7621 tgccatgatg accgttttag ctcaggtgct cgacagggac aactctataa aaaactctac
     7681 ttgtactgat actccaagat tgtttaatgg tacaacaagc cctattagct gctttaatta
     7741 tttaaacact ccatttagag atcttggaga cattcccgat attaccggtg gtggttctat
     7801 gccggttgta actggggatg caaagatagg ggatcagctc gatagtgcag atgaagccct
     7861 tgcccctttt agagtaaata ttggtaatta tgcagggatt gatgagtgtg atgataacaa
     7921 ttcttttaat accgatcagg gtggtggagc cggtatgagt aatgagaaat ttaataatgt
     7981 atttggcgaa cttggcaaca gaagacccag cggccgtacc cctttagcaa gtgcgatagg
     8041 actcgatgat accaatcctg aaaatactgg taccaaaaat gatgttttag gtatatacag
     8101 ggttacatta aattccgata aggaaaatgc tgcatgtaaa gcgcattttt ctgtttttat
     8161 tacagacggt gaagataact gctctggaga ctgtaattct aatcaggctt ttggtacaac
     8221 tggtactggt atggatatgg atgctggtgg ggattcatcg tctggtacct gcgacaacac
     8281 tcttgctccg gcaacaggaa attctaacag aagaagtacc ctgcaggccg caagtttttc
     8341 aaggactcat tttgcaagaa aaccagtaca aggtaatgag tttaatactg aagtaatgtc
     8401 attttttatt ggttggggag taaaggacaa tccccaggct gttagaacat taaatgctgc
     8461 agcgcttttg ggtggaacac atactacggg cttactatta caccgacatc cgcagggttt
     8521 tatttcagga caaaaagatc tggatggaag ccctggagtt cctgctgtgc ttccaaaagc
     8581 agatataacc gagataagat ctagttttcc ctgggtgctg gaaatggcag aaatggaaca
     8641 cagcggtgga gattttagta ataatccact aggtgtaacc ttatcaggtt gtcaaactcc
     8701 aaacgaaacc gggtcttgtt catggaatgg tacagatgta tttgataacg acttttttac
     8761 aggaactgcc ggtacagacc tgccaacagc ttttacaaga actaatgttg gacaaagttt
     8821 tgcatttttt gtagataatc cagctgaact cattgccgca ctggaggcaa tatttgattt
     8881 tacaaaaggc ttttctgcag taggtcaggc tccgcaggtg ccgccatcgg taactgctat
     8941 tggtcttaga gacagggtgc tgctgccaag ttttactcca actataggtg agcctatatg
     9001 gcagggaaga ctggctctat ttggcttttt ggatgatccc gataatccgg gcactaaaat
     9061 cattgtaaga aaaccaaggt cagatggtga atttctggat ggaaatagtc ttgatccaga
     9121 agcattaaga tctgctaata tctttactga taccggggca cttagttcca acgccgaaga
     9181 gtttttctgg gatgcaggta aattgcttgc tgaaagagat attgtatctt ctgcaacttc
     9241 aagaaacctg cttacggtag gagattctac ttcatttggt acactgcaaa tagataacac
     9301 tggctccggt gagtttgatg ctattatcta cacatctggt attgtagcat tcagcagagc
     9361 tctggatccg gaagtgtttg gcataagtga tctggatgtt gatcctgctg gggttgagtc
     9421 tgtaatatca atatgcagtg atatcgctgc agacgcttct tctg
//
LOCUS       DBNGSV010000253         3021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1499489, whole genome shotgun sequence.
ACCESSION   DBNGSV010000253 DBNGSV010000000
VERSION     DBNGSV010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1499489"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..318
                     /locus_tag="AC159U_09365"
     CDS             160..318
                     /locus_tag="AC159U_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337303.1"
                     /translation="MLSSDEEGWNSDDELDDDDEELWDEDDEFFDEEDWDEDDLDEDD
                     EDELRPLN"
     gene            complement(462..545)
                     /locus_tag="AC159U_09370"
     tRNA            complement(462..545)
                     /locus_tag="AC159U_09370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Leu,seq:taa)
     gene            complement(574..648)
                     /locus_tag="AC159U_09375"
     tRNA            complement(574..648)
                     /locus_tag="AC159U_09375"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(614..616),aa:Cys,seq:gca)
     gene            897..1301
                     /locus_tag="AC159U_09380"
     CDS             897..1301
                     /locus_tag="AC159U_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337304.1"
                     /translation="MKKNTIRIMVICIFISLFTLKLYSQTHPAINAYDSYLGAIGNAQ
                     KLDDLNPFISKRKIEVYSKMTPEKKISTLKFKKQIVKFTKRKSINAQTEGDNITLHVD
                     IVDESTNNPAKVEVNVVKENNEWKVDKEKYKF"
     gene            complement(1463..1666)
                     /locus_tag="AC159U_09385"
     CDS             complement(1463..1666)
                     /locus_tag="AC159U_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008231134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR3337305.1"
                     /translation="MAVARITEVIGSSTKSWDDAVNNALKRANKTLRGLTGIEVTKMN
                     AHIKDGKISEYRAHIKITFILEG"
     gene            complement(1835..2173)
                     /locus_tag="AC159U_09390"
     CDS             complement(1835..2173)
                     /locus_tag="AC159U_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337306.1"
                     /translation="MDIYKIPTMSEKNSPESETFTKFFQIWEDAVSESTLKNLKDELF
                     KNSLKLKKDKINEFNQYMNDIMSKNIADSSSRLTEDIQSLNKSMENVQQQLNILTKKI
                     DKLTNSKQKK"
     gene            2385..>3021
                     /locus_tag="AC159U_09395"
     CDS             2385..>3021
                     /locus_tag="AC159U_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3337307.1"
                     /translation="MLTKHSYDSNQSQSIVIILLLLSILSITFPKHTYSQQIEDTSDQ
                     PQTLRGIIRVGCLLPLSGKYEKLGSSALRGVFSAIEKVNDENYNFQVVVKDFEEDAAK
                     LRKAVFELVNEDNVSLIIGPIPSSYSKDVYSTVASLKVPTLIFPISYNLAPENPYFIK
                     YSFPIEKQTEILVKHAVEVVNVNTFGVLYPKTTIGELFKNSFVKNVKKYGKE"
BASE COUNT         1003 a          451 c          507 g         1060 t
ORIGIN      
        1 tccctacata tataatcatg ctacaagtct aactgtaaaa ataacataat catctttatt
       61 ttagatttcc aggtagcttg actttttagg ctctggatgt aatttgtgaa ttattagagt
      121 tgaaaggaga tatttatgtc atttgaacaa tattcttact tgctatcttc agacgaagaa
      181 ggctggaaca gtgatgatga attagatgat gatgatgaag aattgtggga cgaagatgac
      241 gagttctttg atgaagaaga ctgggatgaa gacgatctcg atgaagatga cgaggatgaa
      301 ttaaggcctc tcaactgatt ttgttccata gacttttgat ttaaatttga cctggattga
      361 gtagtaccaa cctctccata gtattatatt aattatagta tagttgtgtc tgaagtatat
      421 ccctgcagtt aaaattccgt agttatataa gaatatataa ctgcccgggg ccggactcga
      481 accggcacag gcttacaccc gagggatttt aagtcccttg cgtctaccaa ttccgccacc
      541 cgggcaagta gtttgattat tgcttagtat atctggaggc ggcgcccgga atcgaaccgg
      601 ggtattgcgg ttttgcagac cgctgcctta cctcttggcc acgccgccac actgaatctg
      661 ccaagtatga acaaaaattc ttttgttttc aagtgtgatt ccaatattat gagataaatg
      721 cgatttataa ctgtatctgt agtgggattt ttgtaattat tgttattgtt ttattaatat
      781 tcagtatagt atcaatataa atgtgctaaa aattttatac ttgatttaaa aaaatctgat
      841 atatataaat aattttgata ttttgacatt tcatatcatt acaaaaggag actttaatga
      901 aaaagaatac tattagaatt atggttatat gtatatttat ttcacttttt acgctcaaat
      961 tatattctca gacacatcct gctataaatg cctatgactc ctatctcggt gctataggta
     1021 atgctcaaaa actggatgat ctgaatccct ttatttctaa aagaaaaatc gaagtatatt
     1081 ccaaaatgac tccggagaag aaaatatcaa cgctcaaatt taaaaagcag atagttaagt
     1141 ttacaaagag aaaatccatt aatgcacaaa ctgaaggtga taatattaca ttgcatgtag
     1201 atattgtcga tgaatctaca aataatcctg ctaaagtcga agttaatgta gtaaaagaaa
     1261 ataacgagtg gaaagttgat aaggaaaaat ataagtttta gtaaacgaaa aacagcaact
     1321 tattattaca atattttcgt tatagttaag cagttagtta ttatcctgcg taagatagat
     1381 agccagatat attattgaat tcaataaata taaaaattaa actagtagga gggctgtttg
     1441 tatgccctcc taaaatactt atttaacctt ctagaataaa tgtaatttta atatgtgccc
     1501 tgtattctga aattttgccg tcttttatat gggcattcat ttttgttact tcaattcctg
     1561 taagaccacg aagggttttg tttgctcttt taagagcatt attaactgca tcgtcccatg
     1621 attttgtgga tgatcctatt acttcggtta tgcgtgcaac tgccataatg atacctccta
     1681 tagttagatt attacttacc ttttatcaaa tattggcttt aaagtcaatg aaaaaattaa
     1741 tataaattgt gtttgtatgt tgtgtagaaa acaaataatt tgctattatc taattaaaaa
     1801 ttaattgttt aaaatctaaa ttgaatagat tttcttattt tttctgtttg gaattagtta
     1861 atttatctat tttctttgta agaatattta gctgctgctg aacgttttcc atggatttgt
     1921 ttagagattg tatatcctcg gtaagtctgc tagaactgtc tgctatattt ttagacataa
     1981 tatcattcat gtactgattg aattcattaa ttttatcttt ttttaattta agtgaatttt
     2041 taaataattc atcctttaga tttttaagag tagattcaga tacagcatcc tcccagattt
     2101 ggaaaaattt ggtgaaggtt tcagattcag gggaattctt ttcgctcatt gtaggtattt
     2161 tgtatatatc caataaattc tacctttaaa ataactatat tcaaacttga gatgatttta
     2221 ttagttccat tactataaat ttgatttaga ttaattatac ttttgcataa tgattgatgt
     2281 ttaacctaat tacatattat gtttgtttat tattttcaag tatttctaga tagttagctc
     2341 aggtttcagt ttacaaaaaa ggtttttaag ggagtatgtg aaaaatgctt acaaagcata
     2401 gctatgattc taaccaatca caatctatag taataatatt acttttatta tccatactat
     2461 ccataacatt tcctaaacac acctactcac agcagattga agatacctct gaccaacccc
     2521 agacactgag gggaattata cgtgttggct gtttgctgcc gcttagcggg aaatatgaaa
     2581 agctcggatc aagcgcgctt agaggagtat tttctgcaat agaaaaagtg aatgatgaaa
     2641 attacaactt tcaggttgtt gtaaaggatt ttgaagagga tgcagccaaa ctcagaaagg
     2701 cagtctttga acttgtaaac gaagacaatg tatctttaat tataggccct atacccagca
     2761 gctattcaaa ggatgtgtat tctacagttg caagcctcaa ggttcccaca ttgatttttc
     2821 cgatttcata taaccttgcg cccgaaaatc cttattttat taaatattcc ttcccaatag
     2881 aaaaacagac tgaaatattg gtaaaacatg ccgtagaggt agttaatgta aatacatttg
     2941 gagtcctata tcctaaaact acaattggag aactgtttaa aaactcgttt gtaaaaaatg
     3001 ttaaaaaata cggaaaagaa a
//
LOCUS       DBNGSV010000254         3957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_806709, whole genome shotgun sequence.
ACCESSION   DBNGSV010000254 DBNGSV010000000
VERSION     DBNGSV010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_806709"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..637)
                     /locus_tag="AC159U_09400"
     CDS             complement(92..637)
                     /locus_tag="AC159U_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337308.1"
                     /translation="MKKFLILSLTLVTALIYSDIAFSRSKARVLDHFKGTVSGSSGSS
                     GSSGSYSSGAGSSSGEASSNVKYFHGDATYYGGETKGKAQLQKIKPHPKRNSCAELWN
                     AYYEKEEINIKVSTRGNYDEQILFFCPTCTDEEHFVKPFTESEYQGHTGMDRIKSCGF
                     DYAVFKGGRGFNEVIVEVPKD"
     gene            complement(774..1388)
                     /locus_tag="AC159U_09405"
     CDS             complement(774..1388)
                     /locus_tag="AC159U_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337309.1"
                     /translation="MNHLIKNISMSLVLGAVWAVITKSVLSVNFIPGSIILPVFYPLL
                     FTGFLFISSLYFDLEIGLSKILITGASSGFVYYLLSLYFPLMSIVLITIIISAGFVNH
                     KKNITSGFISALIKGFIFIPVGIFTADVLIGVFSAFTTAEIISWMILGSVLNVCFMAV
                     IIPLTRLMGIVDKNDNNHQIKSEIDDFRTQTSDIIKEIESISTI"
     gene            complement(1445..2830)
                     /locus_tag="AC159U_09410"
     CDS             complement(1445..2830)
                     /locus_tag="AC159U_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3337310.1"
                     /translation="MFTEKIKKKYVLFSAMLVIAVLAGTSFLTAFPTIPKPAGAAGGA
                     LSWDLKLANPYMLQGDDKDLVLNLKIRGEEITVSERPPVNLVLVLYRSGSMSEKGKLE
                     YAKKAANEIISSLSSRDRLGIVAYSTNVELMFPIQTLDNKATARNMINSLYPTNSTNL
                     SGGLIEGINQIKSVYDKEYLNRVALLSDGLANAGITNINQINNISRAASEKGISLTTM
                     GLGASFDENMLTSIAEYGAGNYYFIESPTQIASIFNREFGQLIKTVAKNPKIRLKLEP
                     GVKVKDVGGYKYFEKDGGIEIVPGDLSSGQERNILIRLDAPTDKVGNNKLARAYLEYD
                     DIVSNEPKSFSEGLDYKVTTKMGLVVKNENKEVGARAASVDVASELYRAAEDYEDGKL
                     DEARQRMTQAITGLEKLNSSPLRTQGTVEQEKALRDAFGDMDANAPAPNTEAGKSLIK
                     KYKTQSREQQK"
     gene            complement(2830..3492)
                     /locus_tag="AC159U_09415"
     CDS             complement(2830..3492)
                     /locus_tag="AC159U_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR3337311.1"
                     /translation="MGIRTRLNTIFKSNINHIVTSAEDPEKIINQSVEEMQDSFDAAK
                     KRVFALSTEIEDNKRYMNNISEQIEYWTDKTHEFVKSDMDENAKQAIRKRRILEDIKR
                     KLEYRITELENKFKAEDRKIREAEGKLIAAKNKRKILLGSYGKTRSAQSADFPQAGFA
                     APNATPDSYEALMRMEERINDEKELIDIKESNEQNLFEKEEKVIDEELQNIKKQIKGG
                     KK"
BASE COUNT         1061 a          821 c          693 g         1382 t
ORIGIN      
        1 aatggaaatg tagacaacat gtcatgaata taattactgt attttaaaaa tcaaaaaact
       61 gaactgcaag aaattatcaa ttatcacaca tttaatcttt tggaacttct acaattactt
      121 cattgaatcc cctgccgcct ttaaacacag cgtagtcgaa tccacatgac ttaatcctgt
      181 ccatacccgt atgcccctga tattcagact ctgtaaaggg ttttacaaaa tgttcttcat
      241 ctgtacaggt aggacagaaa aacaggatct gctcatcata atttcctctt gttgaaacct
      301 tgatgtttat ttcctctttt tcgtagtatg cgttccaaag ctctgcacag ctatttcttt
      361 ttggatgtgg ttttatcttt tgcagctggg ccttgccctt tgtttctcca ccgtagtatg
      421 ttgcatcacc atgaaagtac ttaacatttg atgacgcttc gccacttgat gaacctgcgc
      481 cagagctata tgatccagaa gaacctgatg atccggaaga accggatact gtgcccttga
      541 aatgatctag aacccttgct tttgatctcg agaatgctat atctgagtat attagtgctg
      601 taacaagtgt aaggcttaaa attagaaact tcttcatctt catctcctct tatataaata
      661 tttatttcaa tctataataa aaacttaatt tattgttata ttttactata tttttaaata
      721 gttacaatac ataattaaga agaaacttta attatttaat attttatttt ttgttaaatt
      781 gtactaattg actctatttc tttaataata tcagaggttt gcgtacgaaa atcatcaatt
      841 tcactcttaa tttgatgatt attgtcattt ttatcaacaa tacccataag tcttgttaat
      901 ggaataatta cggccataaa gcacacattt agcactgatc caagtatcat ccatgaaatt
      961 atctctgctg ttgtaaatgc gctaaatacc ccaataagta cgtctgctgt aaaaattcct
     1021 acaggtataa agataaatcc ttttattaaa gcggatataa acccagatgt aatatttttt
     1081 ttgtggttaa caaaacctgc tgaaataatg attgtaatga gaactataga cattagaggg
     1141 aaatataatg ataacagata gtatacaaac ccggaagatg ctcctgttat aagtattttt
     1201 gataatccaa tctcaagatc aaagtataat gagcttataa acaaaaaccc ggtgaaaagc
     1261 agagggtaaa aaacaggcag gattatagac cccggaataa aatttaccga tagaactgat
     1321 tttgtgataa ctgcccagac tgctcccaat actaggctca tactgatatt ttttattaga
     1381 tgattcatgt ttatacccct cttgtataat aaaggagggc aaatgccctc ctcttgtttt
     1441 gactttactt ttgctgctct cttgactgtg tcttatattt tttgataaga ctctttccag
     1501 cctctgtgtt tggcgcaggc gcattagcgt ccatatcacc aaacgcgtcc ctgagcgctt
     1561 tttcctgctc tacagttcct tgtgttctaa gtggagagct gtttagtttt tccaaaccag
     1621 taattgcctg agtcattcgc tgtctggctt catcgagttt tccatcttca taatcttctg
     1681 ctgcgcggta aagctccgaa gcgacgtcta ccgaagcagc ccttgctccc acctctttgt
     1741 tttcattttt aactacaagc cccatttttg tagtaacctt ataatcaaga ccttcactaa
     1801 atgatttcgg ctcatttgaa acaatatcgt catactccag atatgccctt gcaagcttgt
     1861 tgtttcctac cttgtcagta ggagcatcca atcttataag gatgttcctt tcctgtccgc
     1921 ttgagaggtc acccggcaca atctcgatcc cgccgtcttt ttcaaagtat ttataaccgc
     1981 ctacgtcctt aaccttcacg cctggttcaa gcttaagcct tatcttcgga ttctttgcaa
     2041 cggtttttat aagctggccg aattctctgt taaaaataga agctatctgt gtgggggact
     2101 caatgaagta ataatttcct gccccgtact ctgcaatact tgtaagcata ttttcatcaa
     2161 agctagctcc aaggcccata gtggtaaggc ttatgccttt ttccgatgca gcgcgtgaga
     2221 tattatttat ttgattaatg tttgtaatcc ctgcatttgc aagcccgtct gaaagaagag
     2281 ccactctgtt cagatattcc ttatcataga cagatttgat ctggtttatt ccctcgataa
     2341 gcccgcctga aagatttgta gagtttgtgg gatataaaga gttaatcata tttcgcgctg
     2401 tagctttatt atccagcgtc tgtattggaa acataagctc aacgtttgtt gaatatgcca
     2461 caatgccaag cctgtctctt gatgagaggc tggatatgat ctcatttgca gccttctttg
     2521 catattcaag cttacccttc tcactcatcg agccgcttct gtataatacc aatacgaggt
     2581 taactggagg tctttcggat actgtaattt cttcccctct tattttcagg ttcaaaacaa
     2641 gatccttgtc atcaccctga agcatataag ggtttgctag cttaaggtcc catgaaagcg
     2701 cgccgcctgc tgctcccgct ggttttggga ttgttggaaa agccgtaaga aagctggttc
     2761 ctgcaagcac ggcaatgact aacattgcac tgaataaaac atattttttc tttatttttt
     2821 ctgtaaacat tatttttttc ctcctttgat ctgtttttta atattttgaa gctcttcgtc
     2881 tatcaccttt tcttcttttt caaacaagtt ctgctcgttt gattctttta tatcaattag
     2941 ctccttttca tcatttattc tctcttccat tctcattaat gcttcataag agtcaggtgt
     3001 agcattggga gctgcaaatc ctgcctgggg aaagtctgca gattgagcag atctagtttt
     3061 tccataagag ccaagaagga tcttcctctt gttctttgcg gctatgagtt ttccctcggc
     3121 ttctctgatc tttctgtcct ccgctttaaa cttgttctca agctctgtga ttctgtattc
     3181 cagtttcctt ttaatatctt ctagtatcct tctctttctg atcgcctgtt ttgcgttttc
     3241 gtccatgtcg gattttacaa attcatgggt cttgtcagtc cagtattcga tctgctctga
     3301 gatattgttc atatacctct tgttgtcctc tatctctgtg cttagcgcga acactctttt
     3361 ctttgccgca tcaaaactgt cctgcatctc ttctaccgac tgattgataa ttttctcagg
     3421 atcttcggca gaggttacaa tgtggttaat gtttgattta aaaatggtat ttaagcgagt
     3481 tcttattccc ataggctttc tcctcaaaac tgatttttaa ttattaatac gaaaggggtg
     3541 gagaaaggtt tttgggaatt taaaaaaggt cactctgggt gtaaagaaga gtcttaagag
     3601 attcttcgct tcggctcaga atgacccata agtttgtcat gcttgagcgt atgcgaagca
     3661 tctttcttta gattcttcgg cttgcctctg aatgacagag agagtgagat tgtcatgctt
     3721 gagcggaacg actcatacac ccgtcattct gatcgaaacg tagtgtagag aagaatctca
     3781 tgagacttaa ttagattctt cggccccgcc tcagaatgac ttccaagtat gtcatgcttg
     3841 agcgttagcg aagcatcttc atttagattc ttcgagttca cctcagaatg ggcataattg
     3901 caaaaaatgt gtgctgaaaa tggggtttta gcctttattt actatattat aatgaga
//
LOCUS       DBNGSV010000255         5343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1915311, whole genome shotgun sequence.
ACCESSION   DBNGSV010000255 DBNGSV010000000
VERSION     DBNGSV010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1915311"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC159U_09420"
     CDS             complement(<1..433)
                     /locus_tag="AC159U_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3337312.1"
                     /translation="MMNIAYINADPGIPSYGTKGSSIHVQEIIRSMINNGARVDLYTP
                     RVDGNTPSGLEEVKIIKLPDAPKGDLETRENLCIDMNSDLLRYLRDSKKYDLIYERYS
                     LWSYSAIEYAKQNKIPSVLEINAPLIEEEKKYRKLINEDTAL"
     gene            complement(435..1688)
                     /locus_tag="AC159U_09425"
     CDS             complement(435..1688)
                     /locus_tag="AC159U_09425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3337313.1"
                     /translation="MQYSNLTIGYVLKKFPRLSETFILNELLQLEKLGINIIIFSIKN
                     PFDTEDSVISHKYLKQLKAKVIYLPNKRVLKNSKIKILDPASDTIKEQSIESIIDDPD
                     NEDNKNHIRASAIASICTANNITHLHSHFGTASNVAMQASLLANISFSFTTHAKDIYK
                     NDVNYGDLGLKINKSEFVITVSEYNKAFLGNKFKTDDHKIIRLYNGIDVHRFSKDSNK
                     TNANNKDIVLAVGRLEMKKGFEFLIESARILKNKLIDFKCLIVGDGSQRSNLESLIDL
                     YDLSNHVELLGSNNQEQIIELLYSSNVFVLPSIIDDSGDRDVLPTVLIEAMAMKVPVI
                     STKVSAIPEIIENNISGILVEQKDPEKLAESIQILLENPELQLEFSESGRKRVESLFD
                     IEKNAPKLAKLFEQAINKKDNKIAV"
     gene            complement(1675..2979)
                     /locus_tag="AC159U_09430"
     CDS             complement(1675..2979)
                     /locus_tag="AC159U_09430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3337314.1"
                     /translation="MNNKKIAYVVKMFPRLSETFILNEIVELEKMGFNLHIFSSKYPS
                     ETIEHNKVKDVRSQTTHLPEFLGTEKLQILIAFLNVFLKFPGRTLKTFIRALKVKFLN
                     SNSKSLIRFFQCCCIIKDMGECTHLHAHFATVPTRLVTLVNRITGMPYSISTHAKDIY
                     LDNTLNSVSTINRLKNAEFVIANSNTSKADIINALGIEYENKVFTVYNGIDLNKFTYT
                     PAVKTKNLILSVGRLEEKKGFDLLIKSCKKLIDKGISFECCIVGEGSKYKYLKQLISE
                     LNLKKHVKLVGALGHDDLIELYKSATMFVLPCRVLENGDRDVIPNVIKEAMAVGIPVI
                     TTSIPAMEEIIDDNKSGLLAPPENPDLLSKSIEKLFNDKALRLRLSFAARKKIEDRFN
                     INNTIGRLSDLLLSLNDNLASPSPVIALEHKKLQRGLSNAIQ"
     gene            complement(2991..4139)
                     /locus_tag="AC159U_09435"
     CDS             complement(2991..4139)
                     /locus_tag="AC159U_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3337315.1"
                     /translation="MRRNLLISNKISASFPNSSVLLITGAKEINKFNVPKGVDCLTLP
                     SYYKESDSDYEPKSLKVSLDELITLRSGIIKETIKSYSPDIFIVDYAAKGGLGELTKT
                     LKYVNKYSDTICVLGLRDILGDPDYVNKDWNKKKIIEFTRNYYDHIWIYGDPNIYNMA
                     DDCGFPDDIRNKLSFTGYLGQTDLGDYKERRKKYIDNDFGNKKVVLCVVGGGQDGIVL
                     ADTFADTTMPRGYVGVLLTGPYMSGDDKDRLKVKVAHNKTIKLIEFIPDPIKLFSDAK
                     RIISMGGYNTVCELLANKKKALIVPRINSRKEQIIRGQKLSEIGLIDTILPSELSSNN
                     IQSWLCKDITESDSYCRIDLDGLSRIPQLIINIINGECSENHKYKVAL"
     regulatory      complement(4401..4498)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4877..>5343
                     /locus_tag="AC159U_09440"
     CDS             4877..>5343
                     /locus_tag="AC159U_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337316.1"
                     /translation="MRLNQIDLKKVHKIGSRINIWKQDPSVKELGVRTTFVHNRVDPG
                     PCDSQIIIKGLPLVYPDSNGDFLFPTPDQYVVGDIYNPPTITFLEKSFDAAHTYAIVR
                     KVLIMYERVLGKRIKWAWNSDTNSEPLQVYPHANLGRWAYYERDLKSIRFQYY"
BASE COUNT         1598 a          953 c          857 g         1935 t
ORIGIN      
        1 caagtgcggt gtcttcgttt atgagttttc tatacttttt ttcctcttca attaaaggag
       61 cattaatttc cagcacgctt ggtattttat tttgctttgc atattcaatt gccgaataac
      121 tccaaagtga atatcgttca taaattaaat catatttttt ggagtctctc agataacgaa
      181 gcaggtcact gttcatatca atacaaagat tttccctggt ttctaaatca ccttttggtg
      241 catctggaag ttttatgatc tttacttctt caagcccgct tggtgtatta ccatctactc
      301 ttggagtata caagtccact ctagctccat tatttatcat tgacctgata atttcctgaa
      361 catgtatgga gcttcccttt gtgccatatg aaggtattcc ggggtctgca tttatataag
      421 ctatattcat cattttatac cgctatttta ttgtccttct tgttaatcgc ctgttcgaac
      481 agttttgcta atttaggtgc attcttttct atatcaaaca aactctctac tctttttctg
      541 ccactctcag aaaactccaa ttgtaactct gggttttcta ataatatttg tatagattct
      601 gcaagcttct ctggatcttt ttgctcaact aatatcccgg atatattgtt ttctattatt
      661 tctggtattg cgcttacttt ggttgaaatt accggtactt tcattgccat ggcttcaatt
      721 agaaccgtag gtaatacatc tctgtcccct gagtcatcta taatagaagg cagtacaaat
      781 acatttgaac tgtaaagaag ttcaattatc tgttcctgat tatttgatcc caatagctct
      841 acgtggttac ttaaatcata taaatcaatt aatgattcga gattactacg ttggctgcca
      901 tctcccacaa ttaggcactt aaaatcaatt aatttattct taagtatacg ggctgattca
      961 ataaggaatt caaatccctt cttcatttct aacctgccta ccgcaaggac aatatctttg
     1021 ttattggcgt ttgttttatt gctgtcttta ctaaatctgt gaacatctat tccgttgtat
     1081 aatctgataa ttttatgatc atcagtttta aatttatttc ccaaaaacgc tttgttatat
     1141 tcagatactg ttatcacgaa ttccgattta ttaattttaa gaccaagatc accgtaatta
     1201 acatcatttt tatatatatc ttttgcatga gttgtaaaag aaaaactgat atttgccagt
     1261 agtgaagcct gcattgcaac atttgatgct gttccaaaat gagaatgtaa atgagttata
     1321 ttatttgcag tacaaattga ggctatagct gaggctctga tatggttttt gttatcttca
     1381 ttatctggat catctattat actttctata ctttgttcct taatggtatc gctggcagga
     1441 tccaaaattt ttattttgct gtttttaagg acccttttat ttggcaagta aataaccttc
     1501 gcttttaatt gttttaaata tttatgagat atcacggaat cttcagtgtc aaaagggttt
     1561 tttatggaaa agatgattat atttatacca agtttttcta gttgtaataa ctcatttagg
     1621 ataaatgttt cagaaagcct aggaaacttt tttaaaacat aaccaatagt aagattactg
     1681 tattgcattg cttaaacctc tttgtagttt tttatgctcc aaagcaatta cgggtgaggg
     1741 tgaagcgaga ttgtcattta agctaagtag caggtccgat aaccggccta tagtattgtt
     1801 aatattaaat ctgtcttcta ttttctttct tgctgcaaat gaaagcctta gtcttaaggc
     1861 tttatcatta aataatttct caattgattt tgatagcaga tctggattct ccggcggtgc
     1921 caataaacca gatttattat catctattat ttcttccata gctggtatgg aggtagttat
     1981 aacggggata cccacagcca ttgcttcttt gataacattt ggaataacat ccctgtcacc
     2041 gttttctaaa accctgcatg gaagcacaaa cattgttgca ctcttataca gctcaatcaa
     2101 gtcatcgtgg ccaagtgcac caacaagctt tacatgtttt tttaggttta gttcggaaat
     2161 aagttgttta agatatttat atttagaacc ctcgccaaca atgcagcatt caaagctaat
     2221 acctttatct ataagttttt tgcaagattt aatcaaaaga tcaaaccctt ttttctcttc
     2281 taacctgccg acgctaagta ttagattttt agttttaact gcaggcgtat aggtgaattt
     2341 atttaaatcg attccattat agacagtaaa cactttgttt tcatattcga ttcccaatgc
     2401 atttattatg tccgctttac tggtattact attggcaata acaaattctg cattttttaa
     2461 ccgattaatt gtcgaaacag agttaagtgt gttgtctaaa tatatatctt ttgcatgagt
     2521 agaaattgaa tagggcattc ctgtgattcg attaaccagg gtcacaagtc ttgtaggaac
     2581 tgttgcgaaa tgtgcatgta agtgagtgca ctctcccata tccttgataa tacagcagca
     2641 ctgaaaaaat ctaatgagcg atttgctgtt tgaattaaga aatttgacct taagtgcacg
     2701 aataaaagtc tttaatgttc tgccggggaa ctttagaaac acatttagaa aggcaattaa
     2761 tatttgcaac ttttctgtac caagaaattc tggaagatgt gtagtctgag atctgacatc
     2821 ttttaccttg ttgtgctcaa tggtttcaga tggatattta gatgagaaaa tatgaagatt
     2881 gaatcccatt ttttctaatt ctacaatttc atttaatatg aaagtttcag aaagccttgg
     2941 gaacatcttg accacatatg ctattttttt attattcata ggtcttattt ctacagtgca
     3001 accttatatt tgtggttttc ggaacattcc ccgtttatta tatttataat taattgtgga
     3061 attctactaa ggccatcgag gtctattcgg cagtaactat ctgattcagt aatatcttta
     3121 caaagccatg attgtatatt atttgatgac agttcacttg gtaatatagt atcaattaaa
     3181 ccaatttcac ttaatttttg acctctaatt atctgttctt ttcttgaatt tattcttgga
     3241 acaattaaag ccttcttttt gtttgccaac agctcgcaga ccgtgttgta tcctcccata
     3301 gagataatac gtttcgcatc actaaataac ttgattggat ctggtataaa ctcgataagc
     3361 ttaattgttt tgttgtgtgc tacttttact ttaagccggt ctttatcatc accagacatg
     3421 taaggccctg tgagcaaaac tccaacatag cccctgggca ttgtcgtatc agcaaatgta
     3481 tctgctaata ctattccatc ctgccccccg ccaactacac aaagcactac cttcttattg
     3541 ccaaagtcat tatcaatata tttctttctt ctttctttat aatcacccaa atcggtctgc
     3601 ccaagatagc cagtaaatga tagtttgttt ctaatatcat ctggaaatcc gcagtcatct
     3661 gccatgttat aaatatttgg atcaccatag atccaaatat ggtcatagta gtttcttgtg
     3721 aattctatta ttttcttttt gttccaatct ttatttacat aatccggatc acccagaatg
     3781 tctcttaatc cgagtacgca gatcgtgtcg gaatatttgt tgacatattt aagggttttg
     3841 gttaattcgc cgagcccgcc tttggcagcg taatcgacaa tgaaaatgtc tggtgagtag
     3901 gacttgattg tttctttgat aatgccagat cttaaagtta taagctcatc cagtgatact
     3961 tttagtgatt taggctcata atcactatct gattctttgt aataagatgg caatgtaaga
     4021 caatctacgc ccttgggaac attaaattta ttaatctctt tcgcaccggt tatgagtaag
     4081 actgatgaat ttgggaaact tgctgatatt ttattagata tcaaaaggtt tcgtctcata
     4141 tgaccaagcc ctttagtatc atgagagtaa attgctattt tcagttttgc agaaaaaaga
     4201 tcattctctg tgacttgttc tttagctctt agtgttgtaa tcccagagct tttgatcctt
     4261 ttatgcgaag agtatccatc tttatttagt aaagaaattt catcatcctc ctgctaataa
     4321 taattctgaa aggtaaacta taaagggtag gccatataaa cagccagggt taagcataaa
     4381 gctacattac accccacatt ttcggcaacc tagctaccat aaacatcatg ccagtatgat
     4441 gttcttaggc ccacggcttt gcgtcccaac cctttcaagt ggtttgcctt ttcagtacat
     4501 aattcaattt taactccatg ttaatgcaat gtcaataatg ctatggtctg agacatacta
     4561 ttgtattaat catgcatata tgcaaactgt ttgctttttt agtattagaa agaaattgta
     4621 ttatttaata agtggatttt ttaataaaca caattaatat agaaaaagca atcttatgca
     4681 attatactaa ctatcaaaat caataccgac taatctcatc taatttatct gatactcatt
     4741 ttaatgacgc tgataacagt aatatactca atttgtgcaa acagtcttta tgaaaggggt
     4801 aaaactgtat tttattagaa ttaaaacata tagattgata taaaaatgaa gttttaatat
     4861 aattaataag aataaagtga gattaaatca aatcgattta aaaaaagtac acaagatagg
     4921 atctcgaata aatatttgga aacaagatcc aagtgtaaaa gagcttggag tcagaactac
     4981 attcgttcac aatagagtag acccaggccc ttgtgattct cagattatca ttaaggggct
     5041 cccattagta tacccagatt ccaacggcga ttttctgttc ccgacaccgg atcaatatgt
     5101 tgtcggtgac atttataacc ctccgacaat aactttttta gaaaaaagtt ttgatgctgc
     5161 tcacacttac gcaatagttc gcaaggtttt aattatgtat gaaagggtat tgggtaaaag
     5221 aataaaatgg gcatggaatt ccgatacaaa cagtgagccc cttcaggtat atcctcatgc
     5281 aaatcttgga agatgggcct attatgaaag agaccttaaa tctataaggt ttcaatatta
     5341 cta
//
LOCUS       DBNGSV010000256         9629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_460503, whole genome shotgun sequence.
ACCESSION   DBNGSV010000256 DBNGSV010000000
VERSION     DBNGSV010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_460503"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="AC159U_09445"
     CDS             complement(<1..577)
                     /locus_tag="AC159U_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337317.1"
                     /translation="MLTIKKYIKYLEYLLIILITSYLYLSNIELIKFHPDESQWITTS
                     IYFDALIDGNTQSEIWEPGYWTLTQPPVTRYIIGFARKLSGFGTDQINNPWNFSLDRQ
                     TNIAQGALPKPELLKTARTPMTVMAVLSIFLLFYIARKYFGVLSAYILFILLISNNYL
                     AIHLTRALSESALLFFIIVVTILIYIASRILI"
     gene            627..890
                     /locus_tag="AC159U_09450"
     CDS             627..890
                     /locus_tag="AC159U_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3337318.1"
                     /translation="MGLDLVELIISIEEAFDISIDDEEAGKVVTVNDCYKLILSNLAN
                     NNDKSLNNEQVWGIMKNLIITQLGVKPEEVVPDAEFVKDFGAD"
     gene            893..1420
                     /locus_tag="AC159U_09455"
     CDS             893..1420
                     /locus_tag="AC159U_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337319.1"
                     /translation="MKYFYLLLIITFSPIISYAKCMPKHNILSEVQIIDCNGVSFYSS
                     PTLQTFGDDIKGSSRMDKQNKSVLGTIITAKIINSESFWKYPFKSTRESKAFEIGSIR
                     LLFVNDKPETVCPETIDRVDRTENRRIYITLNMCCDTSPKEGLCLLPYSLPLVTEEPN
                     PEKWYKLREGDTLSK"
     gene            1435..1896
                     /locus_tag="AC159U_09460"
     CDS             1435..1896
                     /locus_tag="AC159U_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337320.1"
                     /translation="MLFALLIILIVLAVVYYIVANRPNTWPSIRIGLRGASVIFLFLA
                     LFVCFLLYKQGTAKSDLEKIIPVYPAAKTTSWVPPIRAEQYWLFETKDNLNQISDFYK
                     VLENDPQWEVINGSGNGMLFLKFLKEDIEITLLALRKDDKTEMSYNFKKAE"
     gene            1912..2628
                     /locus_tag="AC159U_09465"
     CDS             1912..2628
                     /locus_tag="AC159U_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337321.1"
                     /translation="MSNKAINNWNKKVHPHGELIKLNERLWYVTGSLPHGNVPRNMVI
                     YRLDSRGLLIHSAVALKEEYMQQVEELGKPEYMIVPNKFHKLDAPLFKKRYPDIKVIC
                     PSTVKANVEKKIPVDFLVENVADEIGIEYHTATGFRPGDFAYELDVAGGKALVFCDLL
                     FNMQHLKGIDGWILKQIGSTGFFGPTWLEKLLMKDKMAIKQLFLNLSEITDLKAILVA
                     HGDPVLENCNEKLKEAASLL"
     gene            2669..3388
                     /locus_tag="AC159U_09470"
     CDS             2669..3388
                     /locus_tag="AC159U_09470"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR3337322.1"
                     /translation="MIKKTLLALNILFLVLAFAKVTLADDDDEFIPKLNVSGKGEIMI
                     EPDLAFVSITVETKALTAAEAAKQNASKTQAVLDAIKAIIGKEDKVTTTGYNLNPYHE
                     YNNTTKKSELKGYTVTNSLNVETKMLKKLGEIIDKSIAAGANNVGSVSFSASKKDSVR
                     KDALAIAVKDARSTAEVVAGAAGVKIVRILSISPSYHYPSPIRRNFKVANTMAAESIP
                     TPIEAGELTVSASVNIVFKIE"
     gene            3464..4021
                     /locus_tag="AC159U_09475"
     CDS             3464..4021
                     /locus_tag="AC159U_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3337323.1"
                     /translation="MAEIPKELEKEVNKWRNFERKRPENIIKQGPGQESIWDYPRPPK
                     VELFTKKIKVEFAGKVIAETTKSYKVMETSSPPCYYIPQEDIQMDFLFKSAYKTLCEW
                     KGSARYWSINVDGNISKNAGWSYPVPWEGFEQIKDHIAFFAGRVDGCYIEDEKVVPQA
                     GDFYGGWITSNIVGPFKGDSGTEHW"
     gene            4254..5240
                     /gene="thiL"
                     /locus_tag="AC159U_09480"
     CDS             4254..5240
                     /gene="thiL"
                     /locus_tag="AC159U_09480"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3337324.1"
                     /translation="MIDELRALKLIKERFGENYEGVAIGIGDDTAAVCTNPDNYLLST
                     TDSQVQGVHFTKNSISARELGRKAVAVSVSDIGAMGGTARYILATAGFSENEDENFMN
                     EFMDGFKDAEQEFSVKLIGGNLTSADKLFVDITVLGEVKKENVVKREGASTGDMILVS
                     GTLGDSALGVKVLERNKDFHNGDSFLINRHNCPTPRLLLGKELAIRKLVSSMIDVSDG
                     LVLDLSRISTDFALGADIKMESIPLSKQYKEQVSDYCDDKYELAISGGEDYELLFTSQ
                     KQNKDQILALSAEIGIQITEIGTITDKATINLLNPDGSKKEIEKRGFVHFTA"
     gene            5241..6590
                     /locus_tag="AC159U_09485"
     CDS             5241..6590
                     /locus_tag="AC159U_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3337325.1"
                     /translation="MEDISLSYNIILILLLLFLSGFFCVAEGSLFSLEKHHIDKLLKE
                     GRKSSLIIQRLLKDPFKLIITILFADEVVNVAYSSVIGLTVGSFMTGYPETYVTLISI
                     AIASPSLLLLGEIGPKTLGVKYPRLLASIISYPLNIFHIAITPIRWVIMILSIGFTRL
                     FGGNIQYEHKKGYSIDEVKALVGLGSDEGIITDIENRLVDNLFKLEKVPSYKIMTPAV
                     DCFFLPSFLSPQSAVYEVKKAGFSRIPVYSGDKDNIIGILYAKDLLSIDAEKINGTNL
                     ESILRPPNFVPRTKMAFELLTQFQQERKHLAIVVDEYGRMDGILTMEDILEELFGEIE
                     DERRVIKEKVVRRDGNGFIIPGSVKIDEFNENYLFPVLRYGGIENLSEEIERSELPSE
                     EFSETLGGFVFDQFGRFPKEGEQVEYNNIIFTVHKIFKKRIAEIKVERNRSEVADVA"
     gene            6580..7809
                     /locus_tag="AC159U_09490"
     CDS             6580..7809
                     /locus_tag="AC159U_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3337326.1"
                     /translation="MSLEIVIGLITFFLFLQALFAGSEIALISCDKAVIKSLADSGSK
                     RAKLVLESFLDIEKFISTTLVGINLSLITSTVILTFYIHKHYGKGSELYTVLILSPLI
                     VIFGQVVPKAIFQKRRNSIILWAIYPIWVGSKVFYPILLVVNVFTRKILNLIGKTENA
                     FITREELINVIEGDRSKPVTDNKKRIIKRIFRFSETTADEIMIPLIEVRAIDENATVK
                     EAIAIINETGHSRIPIYSERIDNITGMLHSFYILGAEPSDKINIYAQPPFFVPDSKRV
                     DELMDEMKTGKGGMAIVVDEYGGAIGAVTLEDILEEVVGEIEDEYDKGTKFWRKISDN
                     EYLLNPKIEIEVINDDLGLELPEGEGDYETLSGFLFMKFGSIPRVSEKIKHNNLQFKI
                     EKATVRSIEEVRLKITK"
     gene            complement(7815..8846)
                     /locus_tag="AC159U_09495"
     CDS             complement(7815..8846)
                     /locus_tag="AC159U_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337327.1"
                     /translation="MINKFLLLLLFLSPLLLTDISAADVTLSQNKIEFGSVNRGTLVK
                     KSLQLKNNADKELAVTLIEFTIPGLIVKSKKSIHAKSTGEVTIELDTSTFNGEVNGLA
                     KLHTNNKSNPEILIGISGKVESRFTFDPREAIFLSAFRWETDKKVGSIILKSRDGKPF
                     EIKQVKGDSDLFTAQFSKDEKENTYKISAKINPESEAGMTQNSLLIETEDEKIRIPVY
                     TFLKEKVYANPPGINYGVIDKKEILAKKELLNFLNQSVFIYKHKGEDFKIEFESVPEY
                     LSIKRTPEQGSASVINIPNQGKTAVFEIIVTPNIDKIKIGKFNDSIKLKTNDKDFPSI
                     EFPVFGEVI"
     gene            complement(8931..9605)
                     /locus_tag="AC159U_09500"
     CDS             complement(8931..9605)
                     /locus_tag="AC159U_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337328.1"
                     /translation="MHSFSLVYFAVLCELQKILSVAVFVLSLGVVGVSDSFAAKGGNG
                     DIAKNKSWSVNIIAYENCPKSENAQRIAVLANFVDDVKTGDTFADLDRRNKIFLVPGD
                     DWRVLESNACDDDGALLQLPSDVSMEYDVFVRLVGKPGSGISAYACAQDPTLTDDDIV
                     CSSAFVKTRYTGKGEPSFTKATNELLKVQGTPLFDPSLSDYFWVWNTNGRPHAQVWFV
                     DADAGL"
BASE COUNT         3240 a         1545 c         1929 g         2915 t
ORIGIN      
        1 atattaaaat ccttgaagct atgtatatca aaattgtcac aacgataata aaaaataata
       61 atgctgattc ggacaatgct cttgttaagt gtattgcaag atagttattc gatattagta
      121 aaataaaaag tatataggcg ctcagtactc caaaatattt tcttgcgata tagaacagaa
      181 gaaaaataga taataccgcc atgactgtca ttggagttct ggcagttttt aataattctg
      241 gctttggtaa agcaccttgt gcaatatttg tttgtctgtc aagactgaaa ttccatggat
      301 tatttatctg atctgttcca aaaccactaa gctttcttgc aaaacctata atgtatcttg
      361 tcacaggtgg ctgagtaagt gtccaatatc ctggttccca tatctcagat tgtgtatttc
      421 catctatgag tgcatcaaaa tatatgcttg ttgtaatcca ctggctttca tcagggtgaa
      481 atttaattag ttctatattt gataaataca aatatgaggt gataagaatt attaataagt
      541 attccaggta cttgatatat tttttaatag ttagcatttt tgagagtata ttatattaat
      601 cataaacaat tttgtattaa taatatatgg gattagattt agtcgaatta ataataagca
      661 ttgaagaagc ttttgatatt tctattgatg atgaagaggc tggcaaagta gtcactgtta
      721 atgactgtta taaactcatc ctaagtaatc ttgccaataa taatgataaa tccttgaata
      781 atgagcaggt gtgggggatt atgaaaaatt taattataac ccaactcgga gtaaaacctg
      841 aagaagtggt accggatgca gaatttgtaa aggattttgg ggcggattaa atatgaaata
      901 tttttactta ttattaataa taacttttag tcccattatt tcctatgcta aatgtatgcc
      961 gaaacataat atattatccg aagtacaaat aatagattgc aatggagttt cattttattc
     1021 atctccaact cttcaaacat ttggcgatga tataaaaggt agttcaagaa tggacaaaca
     1081 aaacaaaagt gttttaggta ctatcattac agctaaaata atcaatagtg aatcattttg
     1141 gaaatatcca ttcaaaagca caagagaatc aaaggcattt gaaatcggtt cgataaggtt
     1201 attatttgta aatgataagc cagaaactgt atgtcctgaa acaattgata gagttgatag
     1261 aacagaaaac aggagaattt atataacact aaatatgtgt tgtgatacat ctccaaaaga
     1321 aggtttgtgt ctacttcctt actcactacc attagtaaca gaagaaccta atccagaaaa
     1381 gtggtataag ctcagggagg gcgatacact atcaaaatga atacttccat ataaatgcta
     1441 tttgcactcc taataatcct aatagtcctt gcagttgtct attacatagt agcaaaccga
     1501 cctaatacct ggccctcgat aagaatagga ctaagaggcg cttcggtcat atttttattc
     1561 ttggcgttat ttgtttgttt tttgctgtac aagcagggca ctgccaaatc agacctggaa
     1621 aagatcatcc cggtttatcc tgctgcaaaa accacttctt gggtgcctcc aataagagcc
     1681 gagcagtact ggctgtttga aacaaaagat aatctcaatc aaatctctga tttctataaa
     1741 gtactggaaa acgatcctca atgggaagta atcaatgggt cgggaaacgg tatgcttttt
     1801 ctgaagtttt tgaaagaaga tattgagatt actctacttg ctctaagaaa agatgataaa
     1861 acagaaatga gctataattt taaaaaagct gaataggata aatttattat tttgagtaac
     1921 aaggcgatta acaactggaa taaaaaagtt catccgcatg gcgagttaat aaagctcaat
     1981 gaaaggcttt ggtatgtgac aggctcactg ccgcacggaa acgtccccag aaatatggtt
     2041 atttacaggc tggacagcag agggctattg atacacagcg cggttgcttt aaaagaagag
     2101 tatatgcagc aggttgagga gcttggaaaa cctgagtata tgattgttcc aaataaattt
     2161 cacaagctgg atgctccgct gtttaaaaag agatatccag atataaaagt tatctgccca
     2221 tcaacagtta aagcaaatgt agaaaaaaaa atcccagtag actttttagt ggaaaatgta
     2281 gcagatgaga tagggataga gtatcataca gctacgggat tcagaccggg tgacttcgcc
     2341 tatgagcttg atgttgcggg aggtaaggcg cttgtgtttt gtgacctact ttttaatatg
     2401 cagcacctaa aaggaataga cggctggata ttaaaacaaa tagggagcac gggatttttt
     2461 ggccctacct ggcttgaaaa acttcttatg aaagataaga tggccatcaa acagcttttt
     2521 ttaaatctct ctgaaattac agacttgaag gcaattttag ttgcacatgg agatcctgtt
     2581 ctggagaatt gtaatgagaa attaaaagaa gctgcatcac ttttgtgatt gtttggttta
     2641 aatataataa attataagga gataaacgat gataaagaaa acgcttttag cactaaatat
     2701 attgttttta gtactggcat ttgccaaggt tacgcttgca gacgatgatg atgaatttat
     2761 accaaagctt aatgtgtcgg gaaagggtga aataatgata gagcctgatt tagcatttgt
     2821 aagcataaca gtggaaacaa aagctctaac ggcggctgaa gctgctaagc aaaatgcttc
     2881 taaaacacag gcagtactag atgccattaa ggctatcatt ggcaaagaag ataaggttac
     2941 tacaacaggc tataacctta acccttacca cgaatataac aacaccacaa agaagtctga
     3001 gcttaaaggc tacaccgtta caaacagcct gaatgttgaa acaaagatgc ttaaaaaact
     3061 tggtgaaata atagacaaat ctattgctgc gggtgcaaat aatgttggtt ctgtgagttt
     3121 tagcgcatca aaaaaagaca gtgttagaaa ggatgccttg gcaatagccg taaaggacgc
     3181 cagaagcaca gcagaagtag tagcaggtgc agcgggtgta aagattgtaa gaattcttag
     3241 catatctcct tcatatcatt acccaagtcc tataagacgg aattttaagg ttgcaaatac
     3301 catggccgcc gaatccattc ctactccaat tgaagcgggc gaacttacag taagtgcaag
     3361 tgttaatatt gtttttaaaa ttgagtaaat agcatttttt tatgaatatt gaatcaattg
     3421 aacatcttta tgatcataat taacagatag taaatatatg attatggcag aaatacccaa
     3481 agaattagaa aaagaagtaa ataaatggcg aaattttgaa aggaagaggc ctgaaaatat
     3541 tattaaacaa ggcccggggc aggaatcaat atgggactat ccaaggcctc caaaagtaga
     3601 gctatttaca aagaaaatca aggttgaatt tgccggaaaa gtgatagctg aaactaccaa
     3661 gagttataag gtcatggaaa cctcaagccc tccatgctac tacatcccac aagaagacat
     3721 tcagatggac tttttattta agagtgcata taaaacactt tgcgaatgga aggggagcgc
     3781 aagatactgg tctataaacg tagatggaaa catttccaaa aacgcgggat ggagctatcc
     3841 agtaccatgg gagggatttg agcagataaa agatcatatt gcgttttttg caggcagggt
     3901 ggatggctgc tatatagaag acgaaaaagt agtgccccag gcaggggatt tctatggtgg
     3961 ctggattact tctaatatag tgggaccatt taagggtgat tcgggtactg agcattggta
     4021 agcgccccta gttattgata aatcagaaag aatttaaatc atccagcctt aaaagccatt
     4081 gacttaatcg ctcaaatgaa ctagaattta attaatgggg tatgaaaacc catctaatta
     4141 tggaagacgg taaatatcga agtagaaaca ggtgtttgta tagtaattat acaaatgccg
     4201 ctctcataaa tcttaatcca caacccaata cagttatatc cagaggatga acaatgatag
     4261 atgagctgcg cgctctaaag cttatcaaag agcgttttgg agaaaactac gaaggtgtgg
     4321 caattggtat tggggatgat accgccgcag tttgcacgaa tccggataat tatctgcttt
     4381 ctacaactga ttctcaggtg caaggggttc atttcaccaa aaatagcatt tctgcaagag
     4441 aacttggaag aaaagccgtg gcagtatctg taagtgatat tggcgctatg ggtggcactg
     4501 ccagatacat tttagcaacg gcggggtttt ctgagaacga ggatgaaaat ttcatgaatg
     4561 aattcatgga tggttttaaa gacgcagaac aggagttttc tgtaaagcta ataggcggta
     4621 atttaacctc ggcagacaag ttatttgtgg atatcacggt acttggtgaa gtgaaaaaag
     4681 agaatgttgt aaaaagagag ggcgcatcta ccggagatat gattttagta agcggcactc
     4741 ttggagactc tgcgcttggc gtaaaagttc tagaacgaaa taaggatttt cataatggtg
     4801 acagttttct cataaacaga cacaactgtc ctactccacg cctattgctt ggcaaagaac
     4861 ttgcaataag aaagcttgtt agttcgatga tagatgtgag tgacgggctt gttctagacc
     4921 ttagtagaat tagcacagat tttgcattag gtgcagacat aaaaatggaa agcattcctc
     4981 tatcaaaaca atataaagaa caagtatcag attattgcga tgataaatat gaattagcta
     5041 taagcggtgg agaggattat gagcttctat ttacgtcaca aaaacaaaac aaggaccaaa
     5101 tcttagcact ttctgcagag attgggattc aaattacaga gattggaact attacagata
     5161 aggcaactat aaatttatta aatcctgacg gatcaaaaaa agagatagaa aaaagggggt
     5221 ttgttcactt taccgcttaa atggaagata tatcgcttag ttataatatc attttaattt
     5281 tattattact ttttctttca gggtttttct gcgtagcaga gggatcttta ttttcactgg
     5341 aaaaacatca catagacaaa cttttaaaag aaggcaggaa aagctcatta attatacaga
     5401 gactgctaaa agacccattt aagcttatta ttacaatact ttttgcagat gaagtggtaa
     5461 acgttgccta ctcaagtgtg attggactta cagtgggcag ttttatgaca ggctatcctg
     5521 aaacctatgt tacattaatt tccattgcca tagcttctcc aagtctgtta ttacttggag
     5581 aaattggtcc aaaaaccctt ggagttaagt atccaaggct tcttgcaagc attatttcct
     5641 atccgcttaa tatatttcac atagccatta caccgataag atgggtaata atgattctat
     5701 cgattgggtt tacaaggtta tttggaggaa atatacagta cgagcataaa aaaggctatt
     5761 caatagatga agtaaaggct cttgttgggc ttggaagcga tgagggaatt ataaccgaca
     5821 ttgaaaacag acttgtagac aacctcttta aacttgaaaa agtaccgtca tataaaatca
     5881 tgactcctgc tgtcgattgt tttttccttc cttcatttct ctcccctcaa agcgcagttt
     5941 atgaggtcaa gaaggcaggc ttttcaagaa taccggtata cagcggtgat aaggataata
     6001 ttataggaat tttatatgca aaggacctgt tatctattga tgcagaaaaa ataaacggta
     6061 caaatctcga aagtattcta aggccgccta actttgtacc aagaacaaag atggcattcg
     6121 aacttcttac acagtttcag caggaaagaa agcatcttgc tatcgtagtt gatgagtatg
     6181 gaaggatgga tggaattctc acaatggaag acatactcga agaacttttt ggtgagatag
     6241 aagacgaaag aagagtcata aaagaaaagg ttgtacgtcg tgacggaaat ggatttatta
     6301 tcccgggtag cgtaaagata gatgaattta atgaaaatta tctgtttcct gtgcttaggt
     6361 atggaggcat agaaaatcta tctgaagaaa ttgagcgttc tgagcttcca tctgaggagt
     6421 ttagtgaaac gcttggagga tttgtatttg atcagtttgg aaggtttcca aaagagggtg
     6481 agcaggttga atataacaac attatattca cagttcataa gatttttaaa aaaagaattg
     6541 cggagattaa agttgaacgc aacaggagtg aggtagcaga tgtcgcttga gatagttata
     6601 ggattaataa ctttttttct ttttctacaa gcgctttttg caggctctga aatagcgctt
     6661 atatcatgtg ataaggctgt aataaaatca cttgccgatt ctggatccaa gagggcaaag
     6721 cttgtactag aatcattttt agatattgaa aaatttataa gtaccacgct tgtaggtatt
     6781 aacctctcac taatcactag cacagtgatc ctgacgtttt atatacataa acattacggc
     6841 aagggaagtg agctttatac tgtcctcatt ctatcaccac taattgttat atttgggcag
     6901 gttgtgccta aagccatatt tcagaaacga agaaacagca tcatactctg ggcaatttat
     6961 cctatctggg taggttcaaa ggttttctac ccgatactgc ttgtcgttaa tgtatttacg
     7021 aggaagattt taaatctaat tggtaaaact gaaaacgctt ttataacaag agaggagctt
     7081 ataaatgtaa ttgagggaga tagatcaaaa ccggtaactg ataataagaa gcgaataata
     7141 aaaaggatat tccgtttttc tgaaaccaca gcggatgaaa taatgattcc tttaattgaa
     7201 gttagagcaa tcgatgaaaa tgccacagtt aaagaggcta ttgcaattat caatgaaacg
     7261 gggcattcaa ggattccaat atacagcgaa agaatagata atattaccgg gatgctccac
     7321 tcgttttata tactgggtgc agagccatcg gacaaaatta atatatatgc ccagccgccg
     7381 ttctttgtgc ctgattccaa aagagtcgat gagcttatgg atgagatgaa gaccggaaag
     7441 ggtgggatgg caatagtggt agatgaatac ggcggggcaa taggcgcagt tacacttgag
     7501 gatatactgg aagaagttgt tggtgagatc gaagatgaat atgataaggg tacaaaattt
     7561 tggagaaaaa tatctgacaa cgaatatcta ttgaatccta agatagaaat agaagtcata
     7621 aacgacgatc ttggccttga gcttccggag ggagaagggg attatgaaac acttagcgga
     7681 tttttattta tgaaatttgg ctctattccc agagtgagtg aaaagataaa acacaataat
     7741 cttcagttta aaattgagaa ggctacagta agatcaattg aggaagtgag gcttaagatt
     7801 actaaatagt atatttagat tacctctcca aataccggga attcaataga aggaaaatcc
     7861 ttatcatttg tttttagttt tatagaatcg ttgaacttac ctatttttat tttatcaata
     7921 ttaggtgtaa caattatttc aaagactgct gttttaccct ggtttggtat attaattact
     7981 gaagcagaac cttgttcggg ggttcttttt atactcagat attcaggaac tgattcaaac
     8041 tcgattttaa aatcttcacc cttatgttta tatataaaaa cagattgatt tagaaaattt
     8101 agaagctctt ttttagcaag tatttccttt ttatcaataa ccccatagtt tatcccgggt
     8161 ggatttgcat aaactttttc ttttagaaat gtataaacag gaatcctgat tttttcatcc
     8221 tcagtttcta ttaacagact gttttgtgtc attccggctt ctgactcagg attaattttg
     8281 gctgaaattt tgtaggtatt ttctttttca tctttactaa attgtgcagt aaacagatct
     8341 gaatcaccct tcacttgttt aatctcaaaa ggcttaccat ctctgctttt aagaataatg
     8401 gagcctactt ttttgtctgt ctcccatctg aaagcgctta aaaaaattgc ttctcttgga
     8461 tcaaatgtaa accttgattc cactttgcca gatatcccaa ttagtatttc gggattactc
     8521 ttattattcg tgtgaagttt tgcaagtcca tttacttcac cattaaaagt ggatgtatct
     8581 aactcaatag ttacttcacc tgtagatttg gcgtgtatgg attttttaga ttttacaatt
     8641 agtcccggaa tagtgaattc aattaatgta actgccagtt ctttatctgc attatttttt
     8701 aattgtaatg acttttttac taaagtgcct ctgttaactg aaccaaattc aattttattt
     8761 tggctgagtg tgacatcagc agcactgata tcagtaagta agagagggct aagaaataat
     8821 agtaataata gaaatttatt tatcaaaatt tgaattgata gttagatgag tgaattgaaa
     8881 aagggccggt aaataccggc cctttttaat taattattaa ttattaaata ttaaagtcct
     8941 gcatctgcgt ctacaaacca aacctgagca tgtggtctgc catttgtgtt ccatacccag
     9001 aaataatctg aaaggcttgg gtcaaaaaga ggtgttccct gtactttaag gagttcattt
     9061 gtggcttttg tgaaactagg ttcgccttta cctgtgtatc tggttttaac aaatgctgag
     9121 gaacaaacaa tgtcatcgtc agtaagcgtt ggatcctgtg cgcatgcata tgcggagata
     9181 ccactaccag gttttccaac tagtcttacg aatacatcat attccatgct cacatctgat
     9241 ggaagctgaa gaagtgcacc gtcgtcgtca caggcattgc tttccagtac tctccagtca
     9301 tcgccgggta ctaagaaaat tttatttctt ctatctaaat ctgcaaatgt gtctcctgtt
     9361 tttacatcat caacaaagtt tgcaagtact gctatacgct gagcattttc gctctttggg
     9421 cagttttcat aagcaataat attaacgctc caggatttgt ttttagcaat gtcgccatta
     9481 ccacctttag ctgcaaatga atcacttact ccaactactc ctaatgataa aacaaaaact
     9541 gctactgata atattttttg gagctcgcat aggacagcaa agtatactaa gctgaaacta
     9601 tgcaaaagaa gttacttaag aatacaaaa
//
LOCUS       DBNGSV010000257         2706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1707419, whole genome shotgun sequence.
ACCESSION   DBNGSV010000257 DBNGSV010000000
VERSION     DBNGSV010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1707419"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="AC159U_09505"
     CDS             complement(<1..249)
                     /locus_tag="AC159U_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337329.1"
                     /translation="MCGIVGYIGDYRKTKQVLLDGLSRLEYRGYDSSGLAVLDNSQTS
                     IIKCKGKVADLQKKAKKARLEGHLGIGHTRWATHGLANE"
     gene            complement(251..1642)
                     /gene="glmU"
                     /locus_tag="AC159U_09510"
     CDS             complement(251..1642)
                     /gene="glmU"
                     /locus_tag="AC159U_09510"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3337330.1"
                     /translation="MAESKRQDFKCLILAAGMGRRMKSQVPKVLHPILGIPLINYVLN
                     TVGSVNPKDIFIVIGHESKLIQNELYNSQVKFVYQKQQLGTGHAVMSSKEQFKNYNGD
                     ILILNGDFPLITSNTLKSFLKQHAENKFSVSVLTGEVDNPDGYGRVIRNGKGEVVGIV
                     EQKDCSLQQKKIREINSGAYIVNSVFLWESLTKLVPDNSQKELYLPQIIDIAYENNKK
                     IGVKKLKDINEILGVNTRSELVCAENLIRNKIVSKHLANGVSILDPDSTYISPNVKIS
                     KDTTIYPNTYIYGDCIIGANCTIGPSCWIKDSMIGSDVTVRMNCFIEQAQIKNNIIIG
                     PFANIRPETVVCDNAKIGNFVELKKTKVGPGSKVPHLSYVGDATLGENVNIGAGTITC
                     NYDGVNKNETIIEDGVFIGSDTMLVAPVKVGKNATTGAGSTITKEVSENALAIERSKQ
                     IEIKNWKRPNKKK"
     gene            complement(1629..1985)
                     /locus_tag="AC159U_09515"
     CDS             complement(1629..1985)
                     /locus_tag="AC159U_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3337331.1"
                     /translation="MSFDPVKKIDKPWGYELWWAVTDKYVGKILSIDKGHKLSLQYHE
                     QKDETLYVQQGKLILEIQAENGQMESKNFLPGDSVRIKPYTRHRLKALQDSMVVEVST
                     PDLEDVIRIEDDYGRK"
     gene            complement(2041..2472)
                     /locus_tag="AC159U_09520"
     CDS             complement(2041..2472)
                     /locus_tag="AC159U_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3337332.1"
                     /translation="MRGVNKVMLIGRLGKDPELRYTTSGQPVANFTLATTEVRGSKDT
                     EKQEYTEWHRIVAWGRLAEICGEYLSKGKMVYVEGSLRTRSWDDNQGNKRWTTEIVAN
                     QMQILSPAGEKQAEADKSDAKLPDDFELDDDSFGSDDDVPF"
BASE COUNT          725 a          591 c          414 g          976 t
ORIGIN      
        1 ttcatttgcc agtccatgtg tagcccaccg ggtatgacca ataccaagat gtccctccaa
       61 ccttgccttt ttcgctttct tttgcaggtc tgctacttta cccttgcatt ttattatact
      121 ggtttggctg ttatctaaaa cggccaatcc cgaagagtca taacctctgt attcaagacg
      181 cgatagccca tctaataaga cttgttttgt ttttctataa tctccgatgt atccgacaat
      241 gccgcacata ttattttttc ttattagggc gtttccagtt ttttatctca atttgcttcg
      301 atctttcaat agcaagcgca ttttcactaa cctcttttgt tatagttgat cctgcaccgg
      361 ttgtagcatt ctttcccacc tttacaggcg ctacgagcat cgtatcactt cctatgaaaa
      421 ccccgtcttc aattattgtc tcatttttat taaccccgtc ataattgcac gtgatagtac
      481 ctgcaccaat atttacgttt tctccaagtg tggcatcacc cacataagat aagtgcggta
      541 cttttgatcc gggaccaact ttggttttct ttagctctac gaaattacca atctttgcat
      601 tatcacaaac taccgtttcg ggccttatat ttgcaaatgg cccgatgata atgttgtttt
      661 ttatttgcgc ctgttctata aaacagttca tccttacagt aacatctgac cctatcattg
      721 aatcttttat ccagcaagat ggaccaattg tacaattagc gcctatgatg caatcgccat
      781 aaatataagt attgggatat atagttgtat cctttgatat ttttacgttt ggtgaaatat
      841 atgttgaatc cgggtctagt attgaaacac catttgctaa atgctttgat acaattttat
      901 ttctaattaa attttcggcg cacacaagct cactccttgt gtttactccc aggatttcat
      961 taatatcttt tagtttctta acacctattt tcttattatt ttcataagct atatctatta
     1021 tttgaggcag gtataactct ttttgtgaat tgtctggaac aagctttgta aggctctccc
     1081 acaaaaaaac cgaattaact atataagctc ctgagtttat ttcccttatc tttttttgtt
     1141 gcaggctgca atccttttgc tcaacaatgc caaccacttc gcctttaccg tttcttataa
     1201 cccgcccata accatcggga ttatctactt cgccggttag cacggatact gagaatttat
     1261 tctcggcatg ctgctttagg aatgatttca gagtgtttga tgttataaga ggaaaatccc
     1321 catttaaaat taatatatca ccattataat ttttaaattg ctccttggat gacataacgg
     1381 catggcctgt tcccagctgc tgtttttggt acacgaactt cacctggctg ttatataact
     1441 cattttgaat aagtttagat tcatgtccta taacaatgaa tatatctttt gggtttacgg
     1501 aaccaacagt atttaaaaca taatttatta gtggaatgcc aagaatagga tgaaggacct
     1561 ttggaacttg tgatttcatt ctcctgccca tgccggctgc tagaatcaaa cacttaaaat
     1621 cctgtctttt actttctgcc atagtcatct tctatcctta ttacatcttc aagatcaggg
     1681 gttgatactt ctactaccat cgaatcttgt agagctttta gtctgtgtct ggtatagggt
     1741 ttaattctta ctgaatctcc agggaggaag ttttttgact ccatctgtcc attttctgcc
     1801 tgaatctcga gtataagctt gccctgctgc acatataaag tttcatcttt ttgttcatga
     1861 tactgcaagc ttagcttgtg tcctttgtct attgatagaa ttttaccaac atacttatct
     1921 gtaaccgccc accaaagctc atagccccac ggtttatcaa tttttttaac cggatcaaat
     1981 gacataatta atttacaaaa tataaagaag gaaaattaaa aatactgtta tttaatacat
     2041 ttaaaatgga acatcatcat cacttccaaa cgaatcatca tctaactcaa agtcgtcggg
     2101 tagtttggca tctgacttat cagcctcagc ttgtttttca cctgccgggc ttaatatctg
     2161 catctgattt gcaactattt cggttgtcca ccttttattt ccctgattat catcccagga
     2221 gcgggtacga agcgagcctt ctacatagac catcttgcct ttgcttaagt attctccgca
     2281 gatttcagcc agtctccccc aggcaactat tctgtgccat tctgtatatt cctgcttttc
     2341 tgtatccttt gatcccctca cctcagtggt agcaagtgta aagttagcaa caggctgccc
     2401 gctagttgta tacctaagct cagggtcttt acccagtctt ccaattaaca ttactttgtt
     2461 tacgcctctc atgtattacc tcttaactaa tataatgtgg attttatcat ctgtatctat
     2521 aaaaatctag ctgagattct atatatgata taatctacaa actagaagat taatcaaata
     2581 cattacaggt taaatcaaaa aagttattaa gtattctacc tgtaagcccc catataaagt
     2641 attctttgta attgtactca aacgcctctt ttataaattc accattgtat ttttttaagt
     2701 tagaca
//
LOCUS       DBNGSV010000258         5399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_253042, whole genome shotgun sequence.
ACCESSION   DBNGSV010000258 DBNGSV010000000
VERSION     DBNGSV010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_253042"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..914
                     /locus_tag="AC159U_09525"
     CDS             93..914
                     /locus_tag="AC159U_09525"
                     /inference="COORDINATES: protein motif:HMM:NF020455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CVNH domain-containing protein"
                     /protein_id="HIR3337333.1"
                     /translation="MYFVCELCDIYSGDISNNDGYLTCEKTNAPPGSYQQSCVNISVS
                     GTTLQAFCKYINKYGEQHWRSINFYDYDLCSDDISNFHGYITCVKTNVPRGSYLDSCI
                     SASKRYGELKAMCRTINGELKETTLNDTSDCTADISNNDGNLTCERTAELPAGSYLQT
                     CTNPSLDDTRLTALCEKRGGGNRSTTLEEIDRCTGDISNYDGYLTCRKTGEMPSGSYQ
                     QSCINPSITSDSGGRTLRALCRTISEDTNATSLREPDSCVGDISNNDGHLICRKD"
     gene            complement(999..1610)
                     /locus_tag="AC159U_09530"
     CDS             complement(999..1610)
                     /locus_tag="AC159U_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337334.1"
                     /translation="MKILNIKIISYLLISFVIIGCSQDKDSADDTAVTEADGPVAIVT
                     FTTGDKCHYYDWQFIYNHQYHERGETEDGTQWMRTEPVNTKEYDTTLRLNRSDIGEVE
                     FASDNLSEITYTLDSSDKASEVTIHTNDGSDITIEVQNSVVLPQDFGYFWPDRTRQEI
                     SAAGIVLTAERIEGDPELRCSWLPIDKSYRNIGKQIKGIKFLN"
     gene            complement(1677..2150)
                     /locus_tag="AC159U_09535"
     CDS             complement(1677..2150)
                     /locus_tag="AC159U_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR3337335.1"
                     /translation="MPMLFTDGSVNTKSKTGFGAYLFLSEFGQSIDLLRKQLKVKRFE
                     NTSSTKLELQTLLWAFSDIKELESKLIVYTDSQNILSLQRRRERLEQNDYRSKNHKLI
                     RNHELYKEFYRTTDNMDYELVKVSGHKVSNQKDDIDKIFTLVDRASRNALRADDY"
     gene            complement(2168..2422)
                     /locus_tag="AC159U_09540"
     CDS             complement(2168..2422)
                     /locus_tag="AC159U_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337336.1"
                     /translation="MKIDFSKDDLEKALSSAGSAHHDYEVNTLNGVRDEMWPGFYAAY
                     VLGRLGDFAPASVLNSLLESAPSNENWAASTAEYVIKELT"
     gene            complement(2578..3000)
                     /locus_tag="AC159U_09545"
     CDS             complement(2578..3000)
                     /locus_tag="AC159U_09545"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3337337.1"
                     /translation="MSLDDHKEMSRRALDMWASNNSDRPEDIFSENYINHQEPDVEGG
                     VSDKSLEAWKELVAGYHESFSNSKVLILMQIAEDNNVATRWEFTSTHTGNFMGLKPTG
                     KQITWTGVQIDRFEGGKIVESWVDWDKYRFLEGLGLVK"
     gene            complement(3054..3866)
                     /locus_tag="AC159U_09550"
     CDS             complement(3054..3866)
                     /locus_tag="AC159U_09550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3337338.1"
                     /translation="MDEVDLLSEKIEREALVSLHECCPSDARKEIGLDLVEVEDCVAA
                     CTTNDPSILLNRALGLGTQKQASAESVQKVAEVYSSREINNYFMHIYEDCLTDDASKL
                     LNSPKFVKKRAWMKFRSDSPEPIEAKSDLRVEQVGHDKSADFGMIVSKAFGMLDISIP
                     LMAGLANDDRWKLFVTYDGDKPAGAGSLFIYENMGWFEWGATLPEFRRRGSQASIMAA
                     RLNLAKELGCKYIFTETGESVEGDPQHSYKNILKAGFRESILRQNYAPAIKN"
     gene            complement(3902..4342)
                     /locus_tag="AC159U_09555"
     CDS             complement(3902..4342)
                     /locus_tag="AC159U_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337339.1"
                     /translation="MEEFDPIKHIDNSVLALDNGQWPNFHDAEVHNLNIWRGDVRPED
                     NVWIGARVDISFELCALQFPYMVYMRFHDCENIELKEFNNQNALYDLKFSFEDRGKLN
                     DGTPMTPYICVEFEQAFGMALSFKCFRIEVLDRKNMDDIEVQQC"
     gene            complement(4342..4683)
                     /locus_tag="AC159U_09560"
     CDS             complement(4342..4683)
                     /locus_tag="AC159U_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR3337340.1"
                     /translation="MCKFLFYKSKQVRRKPKSKNRLPVDDKKLNMGNTYSCAPKYYYD
                     IEFTCIDCGLEQNWTARQQKWWHEKAGGYFFATAVRCRECRKNKRIRSEESRKAYFEG
                     MKKKRESRNKL"
     gene            complement(4771..5157)
                     /locus_tag="AC159U_09565"
     CDS             complement(4771..5157)
                     /locus_tag="AC159U_09565"
                     /inference="COORDINATES: protein motif:HMM:NF018339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HIR3337341.1"
                     /translation="MNIHLASSRNEGGKLMILEKQLLKSIGVQPMSHEKLPDIVVFDE
                     KRNWLFLIEAVTSHGPVSPKRWLELEEAFASCKVDRVYVSAFPNSAEFRKNAADIAWE
                     TEVWIADNPDHMIHFNGDRFLGPHER"
BASE COUNT         1512 a         1242 c          969 g         1676 t
ORIGIN      
        1 aaacctgtac ttctgtatat agtttttctt aagttttaaa tcactcctgt ttattcgcat
       61 cttaacaaaa taccccctct acttagttac ttatgtattt tgtctgcgaa ttatgtgaca
      121 tctacagtgg tgacataagc aacaatgacg gttatctgac ctgtgaaaaa acaaatgcgc
      181 cgccgggctc ctaccagcag tcatgcgtaa atataagcgt atcagggacc acattacaag
      241 ctttttgtaa atatataaac aaatatggcg aacagcattg gagatccatc aatttttatg
      301 attatgatct ctgtagtgat gacatcagca actttcatgg ctacataaca tgcgtaaaaa
      361 caaatgtgcc ccggggctcg tatctcgact cctgcatcag tgctagtaaa cgttatgggg
      421 aattaaaggc catgtgcaga acaataaacg gggaattaaa ggaaacgaca ctgaacgata
      481 catctgattg tacagcagat ataagtaaca atgacggcaa tctgacctgc gagagaacag
      541 ccgaattgcc tgctggctcc tacctgcaga cgtgcacaaa tcccagcttg gatgacacca
      601 ggttgactgc tttatgcgaa aaaagaggag ggggcaatcg ttctactacc cttgaagaga
      661 ttgaccgctg cacaggcgat ataagtaact atgatggata tttgacatgc cggaagacgg
      721 gtgaaatgcc ttcagggtcc taccagcagt cttgtataaa tcccagcata accagcgact
      781 ctggtggaag gacgcttaga gcactttgca gaaccataag cgaagataca aatgcaacat
      841 cactaagaga accagacagc tgtgtaggcg atataagcaa caacgatggc catcttattt
      901 gtcgtaagga ttgattgatg attaatgtta taaagacact gaaacctaag tgcagtaaag
      961 acgtaattcc ttatgatctg gaatgatata ataaatattt agttcagaaa ttttattccc
     1021 tttatctgct tacctatatt tctataagat ttatcaatcg gtagccagga gcagcgtaat
     1081 tctggatctc cctcaatcct ttctgccgtg agtacaatac cggcagcgga tatttcctga
     1141 cgggttctat ccggccagaa gtatccgaaa tcctggggta gcacaactga attttgtact
     1201 tcgatagtta tatcactgcc atcattggta tgaatagtta cttccgatgc tttatctgaa
     1261 gaatcaagtg tataagtaat ctcggataaa ttatcgcttg caaattctac ctcgcctata
     1321 tcgcttctgt taagcctgag agtggtgtca tattccttgg tattcacggg ttcggttctc
     1381 atccactgcg tgccgtcctc ggtttcaccc ctttcatgat attgatgatt atatatgaac
     1441 tgccaatcat aataatgaca cttatctcct gtagtaaatg tgacaatggc aaccgggcca
     1501 tccgcttctg tgacggccgt gtcgtctgcg ctgtccttat cctgactgca gccaataata
     1561 acaaatgaaa ttagtagata actaataatc ttaatattta atattttcat aatattctca
     1621 gaagagtaaa caaattaatt agctaaataa tattttaata tgaataacct tatgtatcaa
     1681 tagtcatctg ctctcagtgc attccttgat gctctgtcca caagtgtaaa aatcttgtct
     1741 atgtcatcct tttgattcga taccttatgt ccgctgactt taactaattc gtaatccata
     1801 ttatcggtcg ttctataaaa ttccttataa agctcatgat tcctaattag tttatgattt
     1861 tttgaacggt aatcattttg ctctaaacgc tctcgccttc tctgtagact caggatgttt
     1921 tgagaatctg tatacactat taatttggat tctaattcct taatatcact aaatgcccag
     1981 agtaatgtct gtaattcgag tttggtggaa gatgtgttct caaaacgttt aactttcaac
     2041 tgctttctaa gaagatcaat agattgtcca aattcggata aaaaaagata tgccccaaat
     2101 cctgttttag atttggtatt cacacttcca tctgtaaaaa gcatcggcat atctagatta
     2161 ggtcttttta agttaactct tttatcacat actctgcagt tgatgctgcc cagttttcat
     2221 tagaaggtgc actttccaat agtgaattta aaacgctagc cggggcaaag tcacccagtc
     2281 taccgagaac gtatgcggca taaaaacccg gccacatttc atccctaaca ccatttaatg
     2341 tgtttacttc atagtcatga tgtgcgctgc ctgctgaaga taaagctttc tcaagatcat
     2401 cttttgaaaa atctattttc atatagtaat acctcctatc aatccagcca aagtcatggc
     2461 ttgttacatg ttttttatac tccacaacat aatcatgtac tgatgcatct atgtattagt
     2521 ttcttttaac aactaagtag attcatagag gtgccgttaa acgtattggg attgggtcta
     2581 tttcacaaga cctagccctt ctaaaaagcg atatttgtcc caatcgaccc agctctcgac
     2641 gattttacca ccttcgaatc ggtcgatctg gacacccgtc caggtaatct gcttaccggt
     2701 tggcttaaga cccatgaaat tgcctgtatg ggtagacgta aactcccacc tcgtggcgac
     2761 attattgtcc tcagcaatct gcatcagaat aagaaccttc gaattggaaa aggactcatg
     2821 ataacctgca accaattctt tccatgcctc caggctttta tccgatacgc cgccttctac
     2881 atctggttcc tgatgattta tataattttc tgaaaagata tcctcgggtc tatctgaatt
     2941 gttactggcc cacatatcta atgcacgcct cgacatctct ttatggtcgt caagtgacat
     3001 cttaccctcc tatccaccct tgtttatcag aaacaaacac tatttattat atgctaattt
     3061 ttaatagcag gtgcgtaatt ctgtctcagt atagattccc tgaatcctgc ctttaagata
     3121 ttcttgtatg agtgctgcgg gtcgccttct acagattctc ctgtttctgt aaaaatatat
     3181 ttacacccaa gctcttttgc cagatttagt cttgcagcca tgatggaagc ctggctaccg
     3241 cgcctgcgaa actccggaag tgtagcaccc cactcgaacc agcccatatt ttcatatata
     3301 aacaatgacc ctgccccggc gggtttatcc ccgtcatagg tcacaaacag tttccaccta
     3361 tcatcatttg caagccctgc cataagcggt atcgatatat ccagcattcc aaacgccttg
     3421 ctaactatca ttccaaaatc tgcagattta tcatggccaa cctgctctac tctaagatcc
     3481 gacttggctt ctataggttc agggctatca gaccgaaatt tcatccaggc ccttttcttt
     3541 acaaactttg gactgtttaa aagcttgctc gcatcgtctg ttaaacagtc ctcatatata
     3601 tgcatgaaat aattatttat ttcccttgat gaataaacct ctgcaacttt ttggacagat
     3661 tctgccgatg cctgtttttg cgtaccaagc ccaagagcgc ggtttaaaag tatactcgga
     3721 tcatttgtag tacaggcagc tacacagtct tctacctcga ccagatcaag accgatctct
     3781 ttcctggcat ctgaaggaca gcactcatgc aggctcacta aagcttcacg ttctattttt
     3841 tcagataata aatctacttc atccataata ttaattttaa aatcaactga agttatctat
     3901 tttaacattg ctgcacttct atatcatcca tattctttct atccagcacc tctatacgaa
     3961 aacatttaaa agacagagcc attccgaatg cctgttcgaa ttctacacat atataaggag
     4021 tcattggtgt gccgtcattt aatttccccc tgtcttcgaa tgagaacttt agatcatata
     4081 aggcattttg gttattaaac tctttaagct ctatattttc gcagtcatga aatctcatat
     4141 agaccatata tgggaactga agggcgcaaa gctcaaatga tatatcgacc ctggcgccga
     4201 tccatacatt gtcctcaggg cgcacatccc ctcgccagat gttaaggtta tgcacctcgg
     4261 catcatgaaa gtttggccac tgcccgttat ccagagcgag tactgaatta tctatatgtt
     4321 ttatcggatc aaactcttcc attacaattt attcctgctt tctctttttt tcttcatgcc
     4381 ttcaaaatat gcctttctag actcttctga tctgattctc ttatttttcc tacattctct
     4441 gcacctgacc gccgttgcga aaaaatatcc ccctgccttt tcatgccacc acttctgctg
     4501 cctagcagtc cagttttgct ccagcccgca gtctatacat gtaaactcta tatcataata
     4561 atacttcggc gcgcaggaat acgtatttcc catatttagc ttcttatcat ccaccggaag
     4621 cctgttctta ctcttcggtt ttcgcctaac ctgctttgat ttataaaata agaatttgca
     4681 catgggttaa taagtatatt tgaaatctgg gttaattgca ccagcatcct gtgtcaatga
     4741 ctgacacaga taattctcac agtttctata tcagcgttca tgaggtccaa gaaaacgatc
     4801 tccattaaaa tggatcatgt gatctgggtt gtctgcaatc cagacctcgg tttcccatgc
     4861 tatatctgcc gcattttttc tgaactctgc actattcggg aaagcagata catataccct
     4921 atctacttta cagcttgcaa aggcctcttc tagctctaac catctcttag gagaaactgg
     4981 cccatgactt gttactgcct caatcaagaa taaccagttg cgtttttcat caaaaaccac
     5041 aatgtcaggc agtttttcgt gtgacatagg ctgaacacca atagacttga gaagctgttt
     5101 ttccaaaatc atgagtttac cgccttcgtt ccgactactg gctaggtgga tattcacatc
     5161 accatagcgt tgcctgactt ttacctgcct acccgacagg cgcgtgcggc gctgcccaaa
     5221 aaggtttcag tgaaaaacgc tgttcataac atcagccgag ccatattggt gacagaggca
     5281 tttcgcaatg gggatttgag cctgcttggt gatgcgatga ccgataggct gcatcaaccg
     5341 tatcgcttga aattgattcc cggggcacag tctgcgatgg atgcggcgag agaagcggg
//
LOCUS       DBNGSV010000259         9159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_530780, whole genome shotgun sequence.
ACCESSION   DBNGSV010000259 DBNGSV010000000
VERSION     DBNGSV010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_530780"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="AC159U_09570"
     CDS             <1..1248
                     /locus_tag="AC159U_09570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337342.1"
                     /translation="ELTLYAFIPFPILGLIIYRFGRFIETGFTKIQNSFSQLTESARG
                     AVSAIKVIKAYVAEDDEKKKFEASSSDYLDKNLKLIKIWAFFQPLITLIAGIAIVVFI
                     WLGGIKTITFDITLGNFAAMLVYLTMLSWPMMALAWAYDLIKRATASLNRINEVFAVK
                     PTPEKVEGLKDFQLNGDIEFKNLSFSYNGKKALDGLNLVIPKGSTFGITGRTASGKTT
                     LIELIMKIPRVPEQSIFINGNDLSGISKNSLRRQIIYIPQETTVFSGTVRDNITFMKP
                     DISQAEIERVTKIAEIYDDITSFPDGFETRVGERGLSLSGGQRQRIALARAILFKPEI
                     LILDDVLSSLDLRTEKLALQNIVDDMRGKTLIAVSSRVPSISGFNQIAVFESGRLIEK
                     GSHNELMANEAIYASLYNIQTIK"
     gene            1263..3305
                     /locus_tag="AC159U_09575"
     CDS             1263..3305
                     /locus_tag="AC159U_09575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337343.1"
                     /translation="MKNTEKKTYDIKILKWLFHFARPYRVFMTISFVLMIFAALLELA
                     IPYLAKIAVDNYITPSWAKIYRTSADEKIFTNITKKYKSTVIELDNKSSLINLKNLSR
                     IEKTNLEKSQAISKNRFIVIDPAAFDKSQQKRLGIILSKNKKIFHSAANVYYGNSNSI
                     RSLSHGDLLFLRKNDVKNVIILSAAILGCMFLIFAFTTGFTYLLNFSGHKIMHSIRTS
                     VFSHILSLPQPFFDRNPVGRLTTRVTNDVNSINEMYTSVLVQFCKDLLVITGILVVMF
                     FLNKELTAIMFLFTLIVAYVAVRFRMRLKNVYRKVRISIAKLNAYVQESIQGIVLIKL
                     YNRQNSNLDRFKKVNNENFRANMDQLFAFATFRPVIEFISVFSVGLILWYGGRNVISL
                     NLSLGALIAFLYYVRMLFRPIQELAERYNIFQSAAAASENLYELINEKPEQRISENSE
                     KDFRGKVEFKNVWFAYDGDNWVLRDISFKIEPGETVALVGLTGSGKTTIVNLLLKFYE
                     ITKGEILIDDMDISNFDPKYLRENISSVFQDTFLMGHSESIDDVAGPAASVPKGIYPV
                     TTNTNGHNISSGEKQLVSLEKAFSKKASFLILDEATSHVDATLEQTIQKEIREAHGKK
                     TTLIIAHRLSNVKDANRIIVIHNGEILEEGAHRELITTNGLYSNLYKLQSEIKSLI"
     gene            complement(3308..3964)
                     /locus_tag="AC159U_09580"
     CDS             complement(3308..3964)
                     /locus_tag="AC159U_09580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3337344.1"
                     /translation="MIKAVIFDMDGVMIDSEPLWEKTEKIMMANRGLVYTPVYREKIV
                     GLGQKDSAILLKDTFGLEDETEEIINSRISILLDIYDEELELVSGLESLLESLSDSPL
                     KVALASSSPIRVINFVLDKFELGKYFDPVVSGDMVENGKPNPDIYLHTAGLIGLNPTE
                     CVVIEDSINGVVSAKAADMNCIAVPDKRLDPSGFKKADVIMDELEMVKLEALMNFRSS
                     "
     gene            complement(4137..4391)
                     /locus_tag="AC159U_09585"
     CDS             complement(4137..4391)
                     /locus_tag="AC159U_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337345.1"
                     /translation="MNKLVILTLLFIGLGFSASASAQTSVFGVDIENRNMNACINIKD
                     DNAKIRDDKFCLKEDTNAPIDGIKIDKRIDNTVIKEFYAV"
     gene            complement(4540..4932)
                     /locus_tag="AC159U_09590"
     CDS             complement(4540..4932)
                     /locus_tag="AC159U_09590"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3337346.1"
                     /translation="MSYESYDEDIEYQQEIEEVVTAPAQNSQSILHISDGERVQLQNY
                     IEYGKYTAFMFYAPWCPSCRKARPQVQASANTHANLVLREINIVDWKSPVKDQYNIKA
                     IPYFKLYDSRESLSLKEQALLLILINIY"
     gene            complement(4999..5097)
                     /locus_tag="AC159U_09595"
     CDS             complement(4999..5097)
                     /locus_tag="AC159U_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3337347.1"
                     /translation="MRIVNMLIIMLLLSQFAMAEIYKCIDASGKTS"
     gene            complement(5113..5625)
                     /locus_tag="AC159U_09600"
     CDS             complement(5113..5625)
                     /locus_tag="AC159U_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337348.1"
                     /translation="MRHKICLMLVITFLCGYTLVIAELKELKSYSLNGSDNIITKTGV
                     EFDSAVSSDGNGSYRINVETPTIIKLFETGDIDIEKAKLIYRADIKTDNVAGSVYLLM
                     WCVFNDKGEYFSRALHDPLTGTTDWKVQQTPFFLQDGENPDNIKLNLVINGKGTVWID
                     NIKLLKAPIN"
     gene            5734..6564
                     /locus_tag="AC159U_09605"
     CDS             5734..6564
                     /locus_tag="AC159U_09605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3337349.1"
                     /translation="MTVAPLFTIIIPTFNRKVFIKTAVDSVLNQTYEDFELIIVDDGS
                     VDGTDKLIKEYTDKRIKYFYQENRGVSSARNKGIKESKGEYLSFLDSDDTWVNTKLER
                     TAEYINRFPDIKIFHSNEVWYRNGNLLNQKIKHKKPDGYVYPYALPLCCIGMSTAVVK
                     RDLFDEIGMFDQSLPACEDYDLWLRATYKYQVKLIPEPLTIKHGGRPDQLSSQHSLDK
                     YRIAALDKILQSGVLNDEQYEKTREELIKKCKIYISGLEKRGKDQEANSIMNISIRYS
                     "
     gene            complement(6551..6952)
                     /locus_tag="AC159U_09610"
     CDS             complement(6551..6952)
                     /locus_tag="AC159U_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIR3337350.1"
                     /translation="MTRKPDIKWVLKETVISIHDMLISEHGGSSGIRDEGLLDSALAR
                     PKNVYNYNEGSSIFEQAASLGYSLIKNHPFVDGNKRIALTITAVFLDLNVWELNASEA
                     EAVLIFTDLAAGSLDEREFSLWLDDNCIKNT"
     gene            complement(6949..7173)
                     /locus_tag="AC159U_09615"
     CDS             complement(6949..7173)
                     /locus_tag="AC159U_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3337351.1"
                     /translation="MHKLKITTVGSSAGVVLPKDVLSRMKAQKGDTLFLIETKDGYTL
                     TTYDPEFITQLESAEKVMKKYRNALKELAK"
     gene            7209..7592
                     /locus_tag="AC159U_09620"
     CDS             7209..7592
                     /locus_tag="AC159U_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3337352.1"
                     /translation="MTHKGSCHCKAVEFEIEAPENIVVQECNCSMCSMTGFLHLIVPK
                     SRFKLLKGGDNLTTYTFNTRVAKHTFCKTCGVKAFYTPRSNPDGYSINLRCLDQCGFK
                     KIEIEKFDGQNWEQNATKLEQLSKD"
     gene            7676..8344
                     /gene="msrA"
                     /locus_tag="AC159U_09625"
     CDS             7676..8344
                     /gene="msrA"
                     /locus_tag="AC159U_09625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3337353.1"
                     /translation="MTSSLPPIDLEKHERVETATFCLGCFWGPDGQFGAMDGVIRTRV
                     GYAGGTMPDPTYHNIGDHIESLEIDFDLDKLSFEEIVSVFWKNHNPISPFWKRQYAKA
                     IFFHNEKQKKIILNSKQKLEKELKEQITTEIIPYERFYQAENYHQKYHLQNHTALLAD
                     FKKIFTNMQQFVDSTASARVNGYINGYGTAINFDKDIERLGLEQSSVDILKHQYFEYK
                     KKAV"
     gene            8356..>9159
                     /locus_tag="AC159U_09630"
     CDS             8356..>9159
                     /locus_tag="AC159U_09630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3337354.1"
                     /translation="MSLKFELNNQQKAAVEYDSGHVLVLAGAGTGKTRTIIARAEHLI
                     ESGIEPQRILMLTFTRRAAQEMVNRLSLRVGERSNMVVAGTFHHFCLYTMRKMSRMFE
                     IEAATVIDRDDQIQLMKLVRAAHRKEGENFPAADKLVEVYSYAKNTNQPLKDYLDKYT
                     EYEDETFEKVVKTIENYGTRKYENNYLDYDDILFKFSKKLHEMPEIRERLRGYYDHIL
                     VDEMQDTNPLQWLILEGLRDPAQLFCVGDDAQSIYSFRGADFKNVHYFTN"
BASE COUNT         2993 a         1728 c         1729 g         2709 t
ORIGIN      
        1 gagcttactc tctacgcctt tattcccttc ccaattcttg gtcttatcat ctataggttt
       61 ggaaggttta ttgaaacagg ttttactaaa atacaaaact cattttcgca acttacagaa
      121 tctgcgcgcg gagcagtctc ggcgattaag gtaataaagg catacgttgc agaggatgat
      181 gaaaagaaga agtttgaggc ttccagcagt gactatctag ataaaaattt aaaactaata
      241 aaaatttggg cctttttcca gcctcttata acacttatcg caggtatagc aatcgtggtt
      301 ttcatatggc ttggaggaat aaaaaccatc acctttgata ttacccttgg taattttgca
      361 gcaatgctgg tttatctgac catgctctcg tggcccatga tggctcttgc ctgggcatac
      421 gatctcataa aaagagcaac tgcatctcta aacaggataa acgaagtttt tgcggttaag
      481 cccacacctg aaaaagtaga gggattgaaa gattttcaat taaacggaga tatagaattc
      541 aaaaatctaa gcttttccta taatggcaaa aaagctctgg acgggttaaa tcttgtaata
      601 cccaaagggt ctacatttgg aataacggga aggacggcat ccggcaaaac cactctcatt
      661 gaacttataa tgaaaatccc ccgtgtgcct gagcaaagca tattcataaa tggaaatgat
      721 ctttcaggca tcagtaaaaa cagcctaaga aggcagatta tctatatccc tcaggaaaca
      781 actgtattta gcggaaccgt aagagacaat atcactttta tgaaacccga tatcagccag
      841 gctgaaattg aacgggtaac caaaattgct gagatttatg atgatatcac cagttttcca
      901 gacggcttcg aaacaagagt aggcgagagg ggacttagcc tttcaggcgg gcagaggcag
      961 agaatagcgc ttgcgcgggc aatacttttt aaacccgaaa tattgatatt ggacgatgtt
     1021 ctaagctcat tggaccttag gactgaaaaa cttgcgctgc aaaatattgt agatgatatg
     1081 agggggaaaa cgcttattgc agtctcgagc agggtgcctt caataagcgg ttttaatcag
     1141 attgccgtat ttgagagcgg aaggctaatt gaaaagggaa gccataatga acttatggca
     1201 aatgaagcca tatatgcaag tctttataat attcagacaa taaaataact gctatgactc
     1261 aagtgaaaaa tactgaaaaa aagacatatg acataaagat attaaagtgg ctcttccatt
     1321 ttgcaagacc atacagggtt tttatgacca tctcatttgt actcatgatc tttgcagcac
     1381 ttctggagct tgcaataccg tatttagcca aaatcgccgt cgataactat ataacccctt
     1441 catgggcaaa gatttataga acttctgcag atgagaaaat atttactaat ataactaaaa
     1501 aatataagag cacggttata gaattagaca ataaatcttc actcattaac ttaaagaatt
     1561 taagcaggat agaaaaaacg aatcttgaaa aatcccaggc tattagcaaa aacaggttta
     1621 tagtaataga tccggctgct tttgataagt ctcagcaaaa aagactcggc ataattttaa
     1681 gcaaaaacaa aaagattttt catagtgcag caaatgttta ttatggaaac tctaattcta
     1741 ttagaagttt aagccatggc gacctgctgt ttttaagaaa aaatgatgta aaaaatgtga
     1801 ttatcctgtc tgctgcaatt cttggatgca tgtttttgat cttcgcattc actaccggtt
     1861 ttacatacct cttaaatttt tcaggccaca agataatgca ctcaataagg acatcggttt
     1921 tctcacacat tctatccctg ccgcagccct tttttgatag aaacccagtt ggaaggctta
     1981 cgacacgggt cacaaatgat gtgaactcca ttaatgagat gtacacctct gtcctggttc
     2041 agttctgtaa ggacttactt gtaataaccg gaatcctggt tgtgatgttt tttctaaata
     2101 aagagcttac ggcaataatg tttttattta cactaatcgt ggcatatgta gccgtaagat
     2161 ttcggatgag acttaaaaat gtatacagga aggtgcgaat ttcgattgca aagctaaacg
     2221 catatgtgca ggaaagcata caggggatag tacttataaa actatacaac cgccagaaca
     2281 gtaaccttga cagatttaaa aaagtaaata atgagaactt cagggccaat atggatcagc
     2341 tttttgcctt tgccacattt aggccggtga ttgaattcat aagcgttttc tccgtgggcc
     2401 ttatactctg gtacggagga agaaatgtaa tatccttaaa cttatccctt ggtgcgctaa
     2461 tagcattttt atattatgtt aggatgcttt tccggccgat tcaggaactt gccgaaagat
     2521 acaacatttt tcagtccgct gctgccgcat ctgaaaatct gtatgagtta ataaacgaaa
     2581 aacctgaaca gagaatttca gaaaactctg aaaaagattt tagaggaaaa gttgaattca
     2641 aaaatgtctg gtttgcctat gacggggaca actgggtgct aagagatatt tcatttaaaa
     2701 ttgagcccgg agagaccgta gcgcttgtgg ggcttacagg ctcgggtaaa acgaccatag
     2761 tgaatctact tcttaaattt tatgagataa caaagggaga aatacttata gacgacatgg
     2821 atattagcaa tttcgatccc aaatacttaa gagagaatat atcttcagta ttccaggaca
     2881 cttttttaat gggccatagc gaaagcatag acgatgtagc aggcccggca gcttctgtgc
     2941 caaaaggaat ttatcctgta acaacaaata caaacggcca caacatatca tctggagaaa
     3001 agcagctggt ctcgctcgaa aaggcatttt ctaaaaaagc cagtttcctc atcctggatg
     3061 aagcaacttc acatgtggac gcaactctcg aacaaaccat tcaaaaagag ataagagaag
     3121 cccatggcaa aaaaaccaca ctcataatcg ctcacaggtt atcaaatgta aaagatgcta
     3181 acaggatcat agttatacat aacggtgaga tactcgaaga aggcgcacat agagagctca
     3241 taactacaaa tgggttatat tcaaacctct ataaactgca gtcagagata aagagcctga
     3301 tctagcctta tgaacttcta aaattcatta acgcttcaag tttaaccatt tccaattcat
     3361 ccataattac atctgctttt ttaaacccgg aaggatcgag tcttttatca ggtactgcaa
     3421 tacaattcat atcagcagct ttagcagaaa caaccccgtt aattgagtct tcaatgacta
     3481 cacattcagt tgggtttaga cctattaaac cggcagtgtg caaataaata tccggattag
     3541 gttttccatt ttctaccata tcccctgata cgaccggatc gaaatattta cctagttcaa
     3601 atttatcgag tacaaagttt ataaccctta tgggtgagct tgatgcaagg gcgactttta
     3661 atgggctgtc ggaaagtgac tccagcagtg attccaaccc gcttacaagc tccagttctt
     3721 catcatagat atccagcaga attgaaatcc ggctgttaat aatttcttca gtctcatcct
     3781 ccaaaccaaa cgtatctttt agtaaaattg cagaatcttt ctgcccgagt cctacaatct
     3841 tttctctgta cacgggtgta tagacaagac ctctattagc catcattatc ttctcggtct
     3901 tttcccacag tggctcgctg tctatcatca ccccgtccat atcaaaaatc acggctttaa
     3961 tcattttttc ataccacctc aaattgagac cataagttac tgaaattttt tcagagtaca
     4021 aatgatatgc ttgctgtaaa aatttaggag ggttggtgaa gcctcatccg aagacaaggc
     4081 ttcatctggg agatggtggg gttaagtggc atataatgtt agtcatttaa attatttcag
     4141 acggcataaa attcctttat gaccgtgtta tctattcttt tatctatctt tattccgtct
     4201 attggtgcat ttgtgtcttc ttttaagcaa aatttatcat cccgtatttt agcattatca
     4261 tccttgatgt ttatgcaagc attcatattt ctattttcta tatcgactcc aaaaacacta
     4321 gtctgcgcac ttgccgaagc tgagaaaccg agtcctataa acagtagtgt taaaattact
     4381 aatttattca ttatcctggt catttatctg tcccatcaaa ttgttattta catatttaga
     4441 caagtgacta aacaaaaaga ttcgtacaat ttatagaaat attaggaaag ctaataaagt
     4501 tttggtgaac agaaaaaagt tatttaagaa ggtcagggtt tagtaaatat ttataagtat
     4561 cagaagaaga gcttgttcct tcagagacaa ggactccctg gaatcataga gcttaaaata
     4621 aggtattgcc tttatattat actgatcctt aacagggctt ttccagtcta caatatttat
     4681 ctctcttaaa accaggttag catgtgtatt tgccgacgcc tgaacctgag gccttgcctt
     4741 tctgcatgac gggcaccatg gggcatagaa cataaaagcc gtgtatttcc catactcaat
     4801 ataattttgt agctgtaccc tctctccatc gcttatatgc agtatgcttt gtgaattttg
     4861 tgcgggtgca gttactactt cttcaatctc ctgctgatat tctatatctt catcatagct
     4921 ttcataagac acattggtat cgttgcctga tggttcgatc tgttttttac tagcgccctc
     4981 agggcatgga agattgcttt aagacgtttt accggaagca tctatgcatt tatagatttc
     5041 tgccattgca aactgtgaaa ggagcaacat gataataagc atattaacta ttcgcatagt
     5101 tgtgttttat atctaattta ttggagcctt gagaagtttt atattatcaa tccagaccgt
     5161 gcccttcccg tttatcacca ggttcaactt tatattgtca ggattttctc catcttgaag
     5221 aaagaatggg gtttgctgta ctttccagtc tgtagtacct gttaggggat catgcaatgc
     5281 ccttgaaaaa tactccccct tatcattaaa tacacaccac ataagcaaat atacactacc
     5341 agccacatta tctgttttta tatctgctct ataaatcaat tttgcttttt ctatatctat
     5401 atctccggtc tcaaacaact ttataattgt tggtgtttca acatttattc tataagagcc
     5461 attcccgtcg ctggacacgg cactgtcaaa ttcaactccg gtttttgtaa ttatattatc
     5521 agagccgttt aaagaataac ttttaagctc ttttaattca gctataacca aagtgtatcc
     5581 acataaaaat gtaatcacga gcattaagca aattttatgt ctcataaaag agtccccccc
     5641 tatatatcaa tcctgatttt atgttaatta tgttttctat cactattaaa tataatacat
     5701 tttattaaat tctgatataa acaaattaaa cttatgactg ttgctccact attcactatt
     5761 attataccaa cttttaatag aaaggtattt atcaaaaccg cagtagactc cgtgcttaat
     5821 cagacatatg aggatttcga gttaataata gtagatgacg gctctgttga tggtacagat
     5881 aaattaatta aggaatatac ggataaaaga atcaaatatt tttatcagga aaatagaggc
     5941 gtgtcttcgg caaggaacaa aggtataaaa gaatctaagg gcgagtattt atcctttctt
     6001 gattcggatg atacgtgggt taatacaaaa ttagaaagaa ctgctgaata cattaataga
     6061 ttccccgata taaaaatatt tcattcaaat gaagtctggt atagaaatgg caatttgctt
     6121 aatcagaaga taaagcataa aaaacctgat ggatatgttt atccttacgc tttgccatta
     6181 tgctgtattg gtatgtccac tgcggttgtt aaaagagatt tattcgatga aataggaatg
     6241 tttgatcaat cactgccggc atgtgaggat tatgacctgt ggcttagggc aacatataaa
     6301 taccaggtta agctcatccc tgaaccacta acaatcaaac acggcggcag gccagatcag
     6361 ctttcttctc agcacagtct tgataagtac agaatcgctg ctttggataa aattctgcaa
     6421 agtggtgtat tgaatgatga gcagtatgaa aaaactaggg aagaattgat aaaaaagtgt
     6481 aagatatata tttcggggtt ggagaaaaga ggtaaagatc aagaagccaa ctcaataatg
     6541 aatatatcaa ttaggtattc ttaatgcaat tgtcatccag ccataaggaa aattcccttt
     6601 catctagact acccgcagca agatcagtaa atatcaatac agcttctgcc tctgatgcat
     6661 tgagctccca tacattcaaa tcaagaaata ccgcagtgat tgtcaaagcg atccttttgt
     6721 tcccatctac aaatggatga ttcttgatta gtgaataacc cagtgaagca gcttgttcga
     6781 atattgatga accttcattg taattgtaaa catttttagg tctggctaaa gcagaatcta
     6841 atagaccttc atcacggatt ccagacgaac ctccatgttc agaaattagc atatcgtgta
     6901 tagagatgac tgtttcttta agtacccatt ttatatcagg ttttcttgtc atttagcaag
     6961 ttctttgagt gcattccggt actttttcat tactttctcc gcagattcca gctgggttat
     7021 aaattcagga tcataggtag tcagagtata tccatcctta gtttctatca ggaaaagagt
     7081 atcacccttc tgtgctttca tacgagataa cacatctttt ggtaaaacta ctcctgcaga
     7141 gcttcctaca gttgtgattt ttaatttatg catgatttta ttataacatt tgttataata
     7201 tattgctaat gacccataaa ggatcctgcc actgcaaagc tgttgagttt gaaatcgaag
     7261 ctcctgaaaa tattgtagtt caggagtgta actgttctat gtgttcaatg acaggatttt
     7321 tgcatttaat agtccccaaa tcccgtttca agctgttaaa gggtggtgat aatctcacca
     7381 cttatacatt taacactcga gttgcaaagc acacattctg taaaacatgc ggagtgaaag
     7441 ccttttacac gccgagatca aaccccgatg ggtacagcat taatctcaga tgtcttgatc
     7501 agtgcggatt taaaaagatc gagatagaaa aatttgatgg gcagaattgg gagcagaatg
     7561 ccaccaaact tgagcaactg tccaaagact agacagaatc tgagcatcta aataattata
     7621 aattgtctca tactttttag attgtatact ctttgaatta acctggaagg aaagattgac
     7681 tagctcacta ccaccaatag atttagaaaa acatgaaaga gtagaaacag ctacattctg
     7741 tttaggctgc ttctggggcc ctgacgggca gtttggggca atggacgggg tgataagaac
     7801 aagagttgga tacgcaggcg gtacaatgcc agacccgact taccataata ttggcgatca
     7861 tatagaatcc ttagaaattg attttgattt agataaattg agttttgaag agattgtttc
     7921 agtattctgg aaaaaccaca accctatttc tccattttgg aaacgccagt atgcaaaggc
     7981 catattcttt cataatgaaa agcaaaaaaa gattatatta aattctaaac agaagttaga
     8041 aaaagagctt aaggagcaaa tcacaaccga gatcatccct tatgaaagat tctaccaagc
     8101 tgagaattat catcaaaaat accatctgca aaaccacact gctctgcttg ccgactttaa
     8161 aaaaatcttt acaaacatgc agcagtttgt agattctaca gcttcagcca gggtaaacgg
     8221 ttatattaac ggatacggaa cggccataaa tttcgataaa gatatagaaa ggctaggtct
     8281 tgagcaaagc tctgtagata tactcaaaca ccagtatttc gaatataaaa agaaggcggt
     8341 ctaaaaacaa agaatatgtc attaaaattc gaactaaata atcagcaaaa agccgctgtg
     8401 gaatatgaca gcgggcatgt gcttgtgctt gcaggagcag gcactggaaa gacaagaact
     8461 atcattgcaa gggctgagca cctgatagag tcagggatag agccgcagag gattctcatg
     8521 ctcactttta caagacgcgc cgcacaggaa atggtaaacc gcctgagcct tagggttggc
     8581 gaaagatcaa acatggttgt agcaggcact tttcaccact tctgtctcta tacgatgaga
     8641 aagatgtcaa gaatgttcga gatagaagcg gcaacggtta ttgacaggga cgatcagatt
     8701 cagcttatga agcttgtaag ggccgctcac aggaaagaag gcgagaactt tcctgctgct
     8761 gataaattag tagaggttta ttcctatgca aaaaacacca accagcctct aaaagattac
     8821 ctggacaagt atacggaata tgaagatgaa acatttgaaa aagtcgttaa gactatagaa
     8881 aattacggta ccagaaaata cgagaacaac tatctggatt atgacgatat tctattcaag
     8941 tttagcaaga agttacatga aatgcctgaa ataagagagc gcctaagagg ttattatgac
     9001 cacattcttg tagacgaaat gcaggacaca aaccccttgc aatggcttat acttgaaggc
     9061 ctgcgtgacc cggctcagct attttgtgta ggtgacgatg cccaaagcat atattcattc
     9121 aggggagcgg actttaaaaa cgtgcattac tttacaaac
//
LOCUS       DBNGSV010000260         6287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_391607, whole genome shotgun sequence.
ACCESSION   DBNGSV010000260 DBNGSV010000000
VERSION     DBNGSV010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_391607"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /gene="glmS"
                     /locus_tag="AC159U_09635"
     CDS             <1..1711
                     /gene="glmS"
                     /locus_tag="AC159U_09635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3337355.1"
                     /translation="QTSIIKCKGKVADLQKKAKKARLEGHLGIGHTRWATHGLANEAN
                     AHPHTSGSTSVVHNGIIENYVELKAQLQKSGFEFISDTDSEILAHLIESGLAAGMSFE
                     DSARAAFNKVEGACAVAAVTQKEPEKLIVLRNFSPVVIGLGENENYIASDIPAIISYT
                     NRIIYLQDGDMAVIEKDHINITDLKGNRLDREEVLVSSDPGSFEKSGFKHFMLKEIYE
                     QPRSALDTLRGKLSSDHSNIELESIKPRQFSKVKKIIALGCGTSYHACLVGKQLIENL
                     SRIPVEVNIASEFRYSNPIIDRSTMVVVVSQSGETADTSEALAEAKRLGAMTLAITNV
                     ENSKISRESDHVIYTSAGTEIGVASTKAFTTQLIVFYMLSNILAVSKKEISDKDLATN
                     IKFLVGLPLLIEKILKLDDKIKELAQKYNNYANFIFLGRGLNYPIALEGALKLKEISY
                     IHAEGYAAGEMKHGPIALVDENVPVVFIAPNNGINFQKIIGNLREIKARNGLVIFLTT
                     ENGADEYREYIDDLIEIPECDYNVSPIASIIPLQLFSYHIARIKGTDIDQPRNLAKVV
                     TVE"
     gene            1803..3077
                     /gene="purD"
                     /locus_tag="AC159U_09640"
     CDS             1803..3077
                     /gene="purD"
                     /locus_tag="AC159U_09640"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3337356.1"
                     /translation="MKVLVIGSGGREHALCWKIKQSPLVKKLYCAPGNAGISKEAECV
                     DISVSDFDSLTAFVKEHGIDLTIVGPEQPLSEGIVDVFENEGLKIFGPSKAAAELEGS
                     KAFSKNLMKKYNIPTASYQTFTDLNDAISWIKKIDTPFVVKADGLAAGKGVVICNTVS
                     EGEKALNSIMKDKIFGTAGSEVVIEEFLTGEEASFFVFTDGKDFVELQSSQDHKAVYD
                     GDKGPNTGGMGAYSPAPIVNETLRKKIIDTVVVPTINAMESEGRTYKGILYIGLMIED
                     QKFKVLEYNCRFGDPEAQPLLFRLKSDIVPIFNSIASSKLGQKKLEWKTGYSVCVVMA
                     SNGYPGSYEKGYEIGGLEDLKDKDDIYIFHAGTKFSNNKVVTSGGRVLGITSLDNELM
                     PAINKAYDTIKMIDSANLFCRTDIGKKAIKYL"
     gene            3216..3587
                     /gene="rpsL"
                     /locus_tag="AC159U_09645"
     CDS             3216..3587
                     /gene="rpsL"
                     /locus_tag="AC159U_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3337357.1"
                     /translation="MPTISQLVREGREQIKKKSKAPALQNSPQKRGVCVRVYTTTPKK
                     PNSALRKVARIRLTNGIEITGYIPGEGHMLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DTTGVENRRKSRSKYGSKKPK"
     gene            3608..4078
                     /gene="rpsG"
                     /locus_tag="AC159U_09650"
     CDS             3608..4078
                     /gene="rpsG"
                     /locus_tag="AC159U_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3337358.1"
                     /translation="MPRKGSVTKRKVTPDSRYGNVAVTKFINSLMQDGKKSKAEHIFY
                     GALDIAGEKLKKEPLEVFEQAMDNVKPLVEVRSRRVGGATYQVPMEVNPFRRQTLAIR
                     WLVAASRSRNANSMIDKLAGEIMDAAQNKGGAIKKKEDTSKMAEANRAFAHYRW"
     gene            4106..6163
                     /gene="fusA"
                     /locus_tag="AC159U_09655"
     CDS             4106..6163
                     /gene="fusA"
                     /locus_tag="AC159U_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3337359.1"
                     /translation="MKKLRNIGIVAHIDAGKTTTTERILFYTGLTYKIGEVHEGTATM
                     DWMEQERERGITITSATTTCFWKDHTINIIDTPGHVDFTIEVERSLKVLDGAVVVFDS
                     VGGVEPQSETVWRQADRYHVPRIAFINKMDKVGADFFKVVDQIRERLSANPVYVHIPI
                     VENDLVAGIIDLIEMKYAVWDDETLGSEYKLVDIPDEYTELAEKGKEQLFEALSDVDE
                     GIMEKYLEGGEISKESIKAALRKGTIDLQIVPVFAGSAFKNKGVQLLLDAIVEYLPCP
                     LDVPPVKGINSDTGEEDTRPADPNADFSALAFKIMTDPYVGQLTYIRVYSGKLSSGST
                     VYNSSSSTRERIGRLLRMHANKREEVAEVVAGDIAAAVGLKSSVTGDTLCDDKSRIIL
                     ESLNFPEPVISIAIEPKTKSDQERLGLSLSKLAAEDPSFKVRYDDETGQTIISGMGEL
                     HLEIIVDRLKREFNVDANIGKPQVAYKETIKASADVEHKFVRQSGGRGQYGHVKIKVD
                     PQERGTGFIFVDEIKGGAIPKEYIPAVEKGLLEAIDTGIVAGYPMVDLKVTLYDGTYH
                     DVDSSEIAFKIAASMALKDAVAKVGGNILEPVMSVEVVVPEDHMGTVIGDLSSRRGKV
                     SGTELRGALQAVKSEVPLSEMFGYATNLRSITEGRGTFTMEFSHYAVLPEMLSEELKA
                     KAV"
     gene            6196..>6287
                     /locus_tag="AC159U_09660"
     CDS             6196..>6287
                     /locus_tag="AC159U_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3337360.1"
                     /translation="MSKQKFERGKPHLNIGTVGHVDHGKTTLTAA"
BASE COUNT         2111 a         1045 c         1469 g         1662 t
ORIGIN      
        1 ccaaaccagt ataataaaat gcaagggtaa agtagcagac ctgcaaaaga aagcgaaaaa
       61 ggcaaggttg gagggacatc ttggtattgg tcatacccgg tgggctacac atggactggc
      121 aaatgaagcg aatgcacacc ctcatacatc gggctcaacc tcagttgttc ataacggaat
      181 tattgaaaac tatgtagagc ttaaagctca gcttcaaaaa agcgggtttg aatttatatc
      241 cgatacggat tcagaaattc ttgctcatct aatagaatcg ggtcttgcag caggaatgag
      301 ttttgaagac tctgcaagag cagcatttaa caaagtcgaa ggcgcctgtg ctgttgcagc
      361 tgtgacgcaa aaagagcccg aaaagttaat tgtgctcagg aacttttctc cagtagtaat
      421 agggcttggc gaaaatgaaa actacattgc ctctgatatt cctgcaatca tttcctatac
      481 aaaccgtatt atatatctgc aggacggcga tatggcagta attgaaaaag atcatatcaa
      541 tataacagac cttaagggta acaggcttga tagagaagag gtacttgtaa gctcggatcc
      601 cggcagtttt gaaaagagcg gctttaagca ttttatgcta aaagagattt atgagcagcc
      661 aagatcggct cttgacacac ttagaggcaa gctaagcagt gaccacagta atattgagct
      721 tgaatctata aagcccagac agtttagtaa agtgaaaaaa attattgcgc ttggctgcgg
      781 cacttcttat catgcgtgtt tagtaggaaa gcagctcatt gaaaatctct caagaattcc
      841 tgtagaggtt aatattgctt cagagtttag atactcaaac cctattattg atagaagcac
      901 tatggttgta gttgtatctc aatccggaga aacagccgat acttcagagg cacttgctga
      961 agcaaaaaga ctgggtgcca tgacgctcgc aattaccaat gtggaaaact ccaaaatctc
     1021 aagagagtcg gaccatgtga tatataccag tgcaggaacc gaaataggtg ttgcctccac
     1081 aaaggctttt acaacacagc ttatagtgtt ttatatgctc tcaaatatat tggcggtatc
     1141 aaaaaaggaa atatcagata aagatttggc cactaacatt aagtttcttg ttggccttcc
     1201 tcttcttata gaaaaaatat taaaactcga tgataagata aaagaacttg cccaaaaata
     1261 taataactac gccaacttca ttttcctagg aagaggtctt aattatccta tagctttaga
     1321 aggagctctt aaactaaaag agatttcata tatccatgca gagggctatg cagctggaga
     1381 aatgaaacat ggcccaatag cgcttgtgga tgagaacgta ccggtagttt tcattgcacc
     1441 aaataatggg attaattttc aaaaaataat tggaaaccta agagagataa aggccagaaa
     1501 cggacttgta atctttctta ctacagaaaa tggcgcagat gagtatcgag aatatataga
     1561 tgatctgata gaaatacctg agtgtgatta caatgtttca cctattgcat cgattattcc
     1621 cctgcagctc ttttcttatc atattgcaag gatcaagggt accgatatag atcagcccag
     1681 aaatcttgca aaagtggtta ctgtggaata gatttgtatg agtttttgat ttatattcca
     1741 tcctcgtaaa agattcatgt attaatagta gcaattctaa tattatttaa gaggtattca
     1801 aaatgaaagt actggtaatt ggaagcggag gcagggagca tgctctttgc tggaagataa
     1861 aacaaagccc gcttgtaaag aaactgtatt gcgcacctgg aaatgcaggt atatcaaagg
     1921 aggcggaatg tgtagatatt tctgtatctg attttgattc ccttacggca tttgtaaaag
     1981 aacatggtat tgatctcaca atagtaggcc ccgagcagcc tttatcagag ggtatagtgg
     2041 atgtttttga aaatgaagga ctcaaaatat tcggtccgtc aaaagcggct gccgagcttg
     2101 aaggaagtaa agccttttca aaaaatctta tgaaaaaata taatattcca acagcttcct
     2161 accaaacctt cactgaccta aatgatgcaa tatcgtggat aaagaaaata gatacaccct
     2221 ttgttgtaaa ggcagacggt cttgctgcag gaaaaggggt agtaatttgt aacaccgtat
     2281 cagagggtga aaaagctctt aattccatta tgaaagataa gatatttggt acagccggta
     2341 gtgaagtagt aattgaagaa tttctaaccg gagaagaggc atcttttttc gttttcaccg
     2401 atggtaagga ttttgtggag cttcagtctt cccaggatca taaagccgta tatgatgggg
     2461 ataaagggcc aaacaccggg ggaatgggtg catacagtcc tgcaccaatt gtaaatgaaa
     2521 cgcttagaaa aaaaattata gatactgttg ttgtaccaac tattaatgcc atggaatcag
     2581 agggtaggac atacaaaggg atcctgtaca tcggacttat gattgaggat caaaaattta
     2641 aggttttaga atataactgc cgttttggag atcccgaagc tcagccgctg ctattcagat
     2701 tgaaaagcga tatagtgccg atattcaatt cgatagcgtc atcaaagctt ggtcaaaaaa
     2761 agctggaatg gaaaaccgga tattccgtat gtgtagtcat ggcctcaaat ggttatcccg
     2821 gaagttatga aaagggttat gagattggtg gtcttgaaga tttaaaggat aaggatgata
     2881 tatatatctt tcatgcagga actaagttta gcaacaataa agttgttaca agtggtggaa
     2941 gagttttggg aataacttct ttagataatg aactcatgcc tgccataaac aaggcttatg
     3001 atactataaa aatgatagat agtgcaaacc ttttttgtag aacagatatt gggaaaaaag
     3061 cgattaagta tctatagtga ttggggatat ttctcaaagt acaatgagat atttatttat
     3121 tgacagattt ttttaaaaaa aagttcatta atatcttgac ttccccctta ttttatgact
     3181 taatttctga ttaacttaca tttcggagac aatatatgcc gacaattagt caattagtta
     3241 gagaaggtag agagcagatt aaaaagaaga gcaaagctcc tgcacttcaa aacagtcctc
     3301 agaaaagagg ggtatgtgtg agggtttata ccactacacc taaaaaacct aactctgcac
     3361 tgagaaaagt agccaggata aggcttacaa atggtataga aattacagga tacattcctg
     3421 gtgaagggca tatgcttcag gagcactcag tagtgctggt aagaggaggc agggtaaagg
     3481 atttgcccgg agtaagatat catattgtaa gaggtacctt agacaccaca ggggtagaga
     3541 acagaaggaa aagcagatcc aaatatgggt cgaagaagcc aaaataagta attaagggtt
     3601 taataatatg ccaagaaaag gttcagtaac taaaagaaaa gtaacgcccg attcacggta
     3661 cggcaatgta gccgttacca aatttataaa ttcgctcatg caagatggca aaaagagcaa
     3721 ggctgagcat atattttacg gggcgcttga tattgcagga gaaaaactaa aaaaagagcc
     3781 tttggaagtg tttgaacagg ctatggacaa tgtaaagcct cttgtagagg ttaggtccag
     3841 aagggttgga ggtgccacat atcaggtgcc tatggaagta aaccccttta gaaggcagac
     3901 acttgcaatc aggtggcttg tcgcagcttc aaggagcaga aatgctaact caatgataga
     3961 caagctggca ggagagatca tggatgctgc tcaaaacaag ggtggtgcaa taaagaaaaa
     4021 agaggatacc agcaagatgg cagaggcaaa cagagcattt gcgcattaca gatggtaata
     4081 gtggttaact gatatgagta atgatatgaa aaaacttaga aatattggaa ttgtagctca
     4141 cattgatgct ggtaagacta caacaactga gagaatcctc ttttacacag gacttacata
     4201 taaaattggt gaagtacatg aaggtactgc aactatggac tggatggagc aggagcgtga
     4261 aagaggtatt acgattacct ctgctactac tacatgtttt tggaaagatc atactataaa
     4321 tattattgat actcctggtc acgtagattt tactattgaa gtggaaaggt cgctaaaagt
     4381 gctcgatggg gctgttgttg tttttgacag cgttggagga gtagagcctc aatcagagac
     4441 tgtctggaga caggccgaca gatatcatgt tccccgaata gcgtttatta acaagatgga
     4501 caaagtaggc gctgatttct ttaaagtggt agatcagata agggagaggc taagtgctaa
     4561 ccccgtttat gttcatatac ccattgtaga gaatgacctg gtagcgggaa taattgattt
     4621 aatagagatg aaatatgcgg tttgggatga tgaaaccctt ggttctgagt acaagcttgt
     4681 agatatacca gatgagtaca cagagcttgc agaaaaagga aaagagcagt tatttgaagc
     4741 cctctcggac gttgatgaag gaattatgga aaaatatctt gagggtggag aaatatcaaa
     4801 agaatcgata aaggcagcgc ttagaaaagg tactatagac cttcagattg tccctgtatt
     4861 tgcaggaagc gcttttaaaa ataagggtgt gcagttactg ctggatgcta ttgtagaata
     4921 cctgccatgc cctctggacg tgccgcctgt gaaggggata aattctgata cgggtgaaga
     4981 ggatacaaga ccagccgatc cgaatgctga tttttcagca cttgcattta aaattatgac
     5041 agacccttat gtgggccagc taacttatat aagagtgtat tccggaaagc tttcatccgg
     5101 aagtacggta tacaattcat ctagtagcac cagagagaga atcggaaggc ttcttagaat
     5161 gcatgcaaat aaaagagaag aggtcgccga ggtggtagct ggtgatattg cagctgcagt
     5221 agggcttaaa agttctgtta ccggtgatac cctttgtgat gacaaaagca gaattatttt
     5281 agaaagcctt aacttcccgg agcccgttat ttcaattgca attgagccaa agaccaaatc
     5341 agatcaggaa agactcggac tctcgctcag caagcttgca gcagaagacc cttcatttaa
     5401 agtaagatat gacgatgaaa caggtcagac tattatttcg gggatgggcg agcttcactt
     5461 ggaaattatt gtagacagac tcaaaagaga atttaatgtt gatgctaaca taggtaagcc
     5521 tcaggtagct tataaggaaa caattaaagc aagcgccgat gttgaacata aatttgtaag
     5581 gcagtccggt ggtagaggtc agtatggtca tgttaaaatc aaagttgatc ctcaggaaag
     5641 aggaaccgga tttatatttg tagatgaaat taaaggcggg gcaataccta aagaatatat
     5701 ccctgctgta gaaaaaggac ttcttgaagc cattgatacc ggcattgtag caggctatcc
     5761 gatggtcgat ttaaaagtaa ctttatacga tggtacttac catgatgttg attcttctga
     5821 aatagcattt aaaatagccg cttccatggc gctcaaagat gcagtggcaa aagtgggtgg
     5881 aaacatactt gagccggtaa tgagtgttga ggttgttgtt cctgaagatc acatgggtac
     5941 ggtaattggt gatctgagtt cgagaagggg taaggttagc ggaactgagc ttagaggcgc
     6001 actacaggct gtaaaatctg aagtgccgct gtctgaaatg tttggatatg ctactaacct
     6061 aagatccata acagagggaa ggggaacgtt tactatggag ttctcacact atgcagtact
     6121 gcctgagatg ttgtctgagg aacttaaggc aaaggctgtt taaattttaa acaaaagcag
     6181 gaggtaagga gtaagatgtc aaagcaaaaa tttgagcgag gcaaaccaca cttaaacata
     6241 ggcacagtgg gacacgtaga ccacgggaag actactctta cagcagc
//
LOCUS       DBNGSV010000261         4274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_946638, whole genome shotgun sequence.
ACCESSION   DBNGSV010000261 DBNGSV010000000
VERSION     DBNGSV010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_946638"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC159U_09665"
     CDS             <1..434
                     /locus_tag="AC159U_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337361.1"
                     /translation="KYANGCNTSLAGCTEGGEIGIVTPIVVSGLLLFTTFVPQSGEVE
                     ECSAGLGSGRIFGIDFITGAAALSRVPGAEALVGGDGSIAGVEGGEGMPTPPSLSYSN
                     NTITMSLAFSGSGVVGGAQFLLWELFQFPAATQTLYWEEVI"
     gene            455..982
                     /locus_tag="AC159U_09670"
     CDS             455..982
                     /locus_tag="AC159U_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3337362.1"
                     /translation="MTRRNKEKGFTLMEVIVVVGIVVIILALAGAFSIKAAYRRNVDS
                     VTTRVSNSLQLTKLQAAKNGVEFRTVFVNNGNELEIITHRGDSNIDSTVWQTADNGVL
                     SVKIDTSVIISNLPAFFEFKPNGIASPSDNVLVIANAGTGTDRCGQVFVSALGRIATI
                     QGHWDGTSCSQVEDE"
     gene            998..1822
                     /locus_tag="AC159U_09675"
     CDS             998..1822
                     /locus_tag="AC159U_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3337363.1"
                     /translation="MIKTRDIYIFIFRITRYVGWSIAPLVFLTGLYYSKAGLASISSL
                     ELVLLSFPYAILLFGINDIYDHQSDLLNPRKSNIPIGGEEKRLIVVISGVTAVLMILA
                     SALTLNIENIIITSVLLLTSYKYSSGQYRLKEIPVIDSISNGLIFLLVFALGYSFGDT
                     VFNIPLKIYFVALCVAAIHAFGTVVDYEVDKKVGHNTFCVFVGKRFTLLFIFSAFLSA
                     YYFAEIGRNFINYYFVFCVFLTVIVFIKPVHRIAYNCFKLLFAGFIVTSLIFLFTY"
     gene            1881..2069
                     /gene="rpsU"
                     /locus_tag="AC159U_09680"
     CDS             1881..2069
                     /gene="rpsU"
                     /locus_tag="AC159U_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3337364.1"
                     /translation="MAVVFVKSGETIDSALRRFSSMINKEGILFELKKRAYFEKPSEK
                     KKKKLYASKSKQKKNKKR"
     gene            2073..3011
                     /locus_tag="AC159U_09685"
     CDS             2073..3011
                     /locus_tag="AC159U_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HIR3337365.1"
                     /translation="MKTFSLAALFCSCLLFTTSCSYGEDLEKDVLPKKKDNISSASKS
                     STTGDGALVDDDIEIDEQLLAQNVTEVDLSFPETKSITIETITSEDGIKTLEVAVVLK
                     KGDVSNFEITYRWLHNNEEIEGELENSIPWLDEYKKGDIITVNVIPANTDLEVSFVTE
                     AEFVIPNQPPVITSEPPAEIIGEKKFFEYQVETEDPDGDEVEISLKKAPKGMKIEPAT
                     GLIEWDYTEVKPGSEFNIEILATDTDGGSYTQTITLTIPSGEDGQEGELEQAREDEGQ
                     LNEDEVDAEEEPDEDLQEDFIEEETEDTEDADTEEF"
     gene            3113..3802
                     /locus_tag="AC159U_09690"
     CDS             3113..3802
                     /locus_tag="AC159U_09690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337366.1"
                     /translation="MNNHDSCIKLDRISKSYFDGDNEIRIVENVNAGFQDSKISLILG
                     RSGSGKSTLLNLLSGIDIPSNGSVIINGIALDDLNEQDRTLFRRKNIGIVFQSFHLIP
                     SLTVIENVLLPLELNSLDNINGYDIAKDLLEKVGLIEKSDVFPDTLSGGQKQRAAIAR
                     AIVHDPQIILADEPTGNLDNNTRDQIIEVLFGLVRDKNKTLIMATHSVDLINRSDRAY
                     MIENHYLKKIK"
     gene            3846..>4274
                     /locus_tag="AC159U_09695"
     CDS             3846..>4274
                     /locus_tag="AC159U_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3337367.1"
                     /translation="MFTKSKLLKISLNHYLKHKIQSVLLILGISLGVAVIVAIDIANI
                     SARKSFDKSLKSISSDITHSIYSTNSRLKEEIYFDLRKELGFKNIVPIIERTVFIDQF
                     ANEQFKIIGIDPFSENSQLKFFQNQSISLNSKSFEQFLTEQ"
BASE COUNT         1411 a          649 c          888 g         1326 t
ORIGIN      
        1 tgaagtatgc aaatggctgc aatacaagtc ttgcaggttg tacagaaggt ggcgaaatag
       61 gtattgttac tccaattgtg gtaagcggac tgttgctgtt tacaacattt gttccgcaat
      121 caggtgaggt tgaagaatgt tctgccggac taggttctgg aaggattttc ggaatagatt
      181 tcattacagg agcggcagca ctatcaagag taccgggtgc agaagcttta gtaggtggtg
      241 acggtagtat cgccggtgtt gagggtggtg aaggcatgcc cacaccgcct agtttatcat
      301 actcaaacaa tacgataact atgagtctgg cattttccgg aagtggtgta gttggagggg
      361 ctcaattttt gttatgggaa ttatttcaat tcccagcagc gacacagaca ctctattggg
      421 aagaggttat atagtgtata tttagatcat ggaaatgacc agacgaaata aagaaaaggg
      481 ctttaccctt atggaagtta tcgtggtggt tggtattgtc gtcatcattt tagctctagc
      541 aggggcattc tcgattaagg ctgcgtatag aaggaatgta gattcggtaa caactcgtgt
      601 gtctaattcg cttcagctca caaaactgca ggctgcaaaa aacggagtag aatttagaac
      661 tgtatttgtt aataatggga atgaacttga gataataact cacagaggtg acagcaatat
      721 tgattctacg gtatggcaga cagccgataa tggtgtattg agtgttaaaa tagatacttc
      781 tgttataata tcaaaccttc cggcattttt tgagtttaag cccaatggaa ttgccagccc
      841 ttcagataat gtacttgtaa tagcaaatgc aggcactggt acggataggt gcgggcaggt
      901 atttgtgagc gcacttggca gaattgcgac tattcagggt cactgggatg gcaccagctg
      961 ctcacaggta gaagacgagt aaaattctcg gcatagtttg attaaaacga gagatattta
     1021 catatttatt tttagaataa caagatatgt aggctggagt atagctcctc ttgtttttct
     1081 tactggtctt tattattcca aagcaggttt ggcaagtata tcttctttgg agttggtatt
     1141 actttcattc ccatatgcca ttttactttt tggtataaat gatatatacg atcatcaatc
     1201 tgatctgcta aatccaagaa aaagcaatat tcctattgga ggagaggaaa agagattaat
     1261 tgtagttata tccggtgtta cagcagtgct gatgatattg gcttcagctc taacattaaa
     1321 tattgaaaat attattatta cttctgttct tttgcttaca tcttataaat attcatccgg
     1381 acaatataga ttaaaagaaa ttccggttat agactctata tcaaacgggt tgatattcct
     1441 attggtattt gcccttggtt acagctttgg cgatacggtt tttaacatac ctcttaaaat
     1501 atattttgtt gcgctttgtg ttgctgcgat tcatgcattt ggtacagtgg ttgattatga
     1561 agtggataaa aaagtagggc ataacacttt ttgtgtattt gttggaaaga ggtttacact
     1621 gctattcata tttagtgcat tcttgtctgc ttattatttt gcggaaattg gaagaaattt
     1681 tataaactat tattttgtat tctgtgtttt tcttacagtt atagtgttta ttaagcccgt
     1741 gcatagaata gcatataatt gttttaagct tttatttgca ggctttatcg ttacatcatt
     1801 aatattttta ttcacatact aaaaatttct tataaccatg ttattataaa tagcttaaac
     1861 caagataagg agttgagtat atggcagtag tttttgtaaa aagcggtgaa acaattgaca
     1921 gtgcgcttag aagattttca agcatgataa ataaagaagg aattctattt gaacttaaga
     1981 aaagagccta ctttgaaaaa ccaagcgaga aaaagaagaa aaaactctat gcaagtaagt
     2041 caaaacagaa aaaaaataag aagaggtaac ttatgaagac tttttcttta gctgctttat
     2101 tttgcagctg cttgttattt actacatcct gtagctatgg tgaagatctt gaaaaggatg
     2161 tacttcctaa aaagaaggat aatataagta gtgctagtaa aagcagtact accggtgatg
     2221 gtgctttagt tgacgatgat atagaaattg atgagcagct gttagctcag aatgttactg
     2281 aagttgattt atcatttccc gaaacaaaat caattaccat tgaaactata accagcgaag
     2341 atggtattaa aacgttagag gttgctgtag ttctaaaaaa gggtgatgtt agtaattttg
     2401 agattacata taggtggctt cataataacg aagagattga gggtgaactg gagaattcta
     2461 tcccatggct ggatgaatat aaaaaaggcg atataattac agtaaatgtt atccccgcta
     2521 atactgatct tgaagtttca tttgtaaccg aggctgaatt tgtaataccc aatcaacccc
     2581 ctgtcattac ttcggaacct ccggcagaga taatcggaga gaaaaagttt tttgaatacc
     2641 aggttgagac agaagatcca gatggcgatg aggttgaaat tagccttaaa aaagctccca
     2701 agggaatgaa aatagagcct gctacgggtc taatagagtg ggattatact gaagtaaaac
     2761 ccggcagtga atttaatata gagattttag caactgatac cgacggtggt agctatacac
     2821 agacaattac ccttacaata ccaagtggtg aagatggaca agagggtgag ctagagcagg
     2881 ctcgcgaaga tgaaggccag ctaaacgaag atgaagtgga tgctgaagaa gagccagatg
     2941 aagacctgca agaagatttc atagaagaag aaacggaaga tactgaagac gctgatacag
     3001 aagaatttta aaaacctctg caataattat ttctagtagt tcttgatatt attacctgct
     3061 atttatacac tctcgatata acctgtttgt gatagggtat ccttagtatt caatgaataa
     3121 tcatgatagc tgtatcaaat tagaccgaat ttccaagtcg tactttgacg gcgataatga
     3181 aataaggatt gttgaaaatg tgaatgcggg atttcaggac tcaaaaatct ctctaatcct
     3241 tggccgcagc ggaagtggaa agagtacttt gttaaacctt ctatccggta ttgatatccc
     3301 ctcaaatgga agtgtcataa taaatggtat tgctctcgat gatctgaatg aacaggatag
     3361 gacacttttt cgcagaaaga atatcgggat agtgtttcag tcctttcatt taattccatc
     3421 actaactgtg attgaaaatg ttttgctccc tttggaactt aatagcttag ataacataaa
     3481 tggatacgat attgctaaag atttgcttga aaaggtagga ctaatagaaa aatctgatgt
     3541 ttttccagat actctatcag gtggtcaaaa gcaaagagca gctattgcta gagccattgt
     3601 gcatgatcca caaattattt tagcagacga acctaccggc aaccttgata ataatacaag
     3661 agatcagata attgaggttc tttttggatt agtaagggat aaaaataaaa cacttattat
     3721 ggctacacat agtgtagacc tgataaacag gagtgacaga gcatatatga ttgaaaacca
     3781 ttatttgaaa aaaattaaat agttctattt aagattactg taataacagt ccatattaat
     3841 tttgtatgtt tacaaaatct aaattactga aaatttcatt aaaccactat ctaaaacaca
     3901 aaatacagtc ggtactcctt atactgggta tttctctggg agtagctgtg attgtagcaa
     3961 tagacatcgc aaacataagc gccagaaaat cctttgataa gtctctaaaa agtattagca
     4021 gtgatatcac tcattctata tacagtacaa attcaagact taaagaagaa atctattttg
     4081 atttaaggaa agaattaggg tttaagaata ttgtcccgat tatagagcgt acagtcttta
     4141 tagatcagtt tgcaaatgag caattcaaga tcataggcat tgatccattt tcagaaaatt
     4201 ctcaactaaa attttttcaa aatcagagta tcagtctaaa ttcaaaatcc tttgagcaat
     4261 ttttgacaga gcaa
//
LOCUS       DBNGSV010000262         6404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_600158, whole genome shotgun sequence.
ACCESSION   DBNGSV010000262 DBNGSV010000000
VERSION     DBNGSV010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_600158"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..241)
                     /locus_tag="AC159U_09700"
     tRNA            complement(168..241)
                     /locus_tag="AC159U_09700"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Ala,seq:tgc)
     gene            complement(331..407)
                     /locus_tag="AC159U_09705"
     tRNA            complement(331..407)
                     /locus_tag="AC159U_09705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(371..373),aa:Ile,seq:gat)
     gene            complement(547..2122)
                     /locus_tag="AC159U_09710"
     rRNA            complement(547..2122)
                     /locus_tag="AC159U_09710"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2455..3246
                     /locus_tag="AC159U_09715"
     CDS             2455..3246
                     /locus_tag="AC159U_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3337368.1"
                     /translation="MGKTICVTNQKGGVGKTTTVINLGCSLAAAQKKTLIIDLDPQAN
                     ATSGVGIDKNESETTVYDVLLGRSDIRNSIKKVHQSVLNDYLFLCPSNAELTGAEIEL
                     IGVENREWKLKEAISKIVLDFDYILIDCPPSLNLLTINSLAAADSVLIPVQCEYYALE
                     GLSQLNRTINLIKERLNSDIQVEGYLLTLFDSRNRLSSVIVNEVRTYFKDKVFDTIVP
                     RNVRLAECPSHGLPILLYDATSKGAQSYISLAREIIENNNGGYRS"
     gene            3243..4103
                     /locus_tag="AC159U_09720"
     CDS             3243..4103
                     /locus_tag="AC159U_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3337369.1"
                     /translation="MSKKGVLGKGLDALIVKDEKKNSEPSDKNATKVSIHKIFPNPDQ
                     PRKHFTDKSIEELSASIAENGVIQPIIVRNTDRGLEIIAGERRWRAAQKAGVNEVPVI
                     VRQVNDQQSLELALIENLQREDLNPVEEARAYEMLISQYDLTHEEIAKQIGKDRTTVT
                     NSLRLLKLSTRALDALSSGQISQGHARALVSLEDAAKINMVLEKIISGGLSVRNTEAL
                     IKKLLSSTEKDTSAQPAEEDLNIKFFTNQLKSALSTNVRIVNKKGKGKIEIDYNSSDD
                     FDRILKKLIQ"
     gene            complement(4166..4711)
                     /locus_tag="AC159U_09725"
     CDS             complement(4166..4711)
                     /locus_tag="AC159U_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR3337370.1"
                     /translation="MNCLNCNTELDSKVFGDLEVDECAKCKSIWFDSDELRKAKDLTD
                     ENLNWMDFEIWKHEHLFKTKSKDLPCPKCSKALVSINYADTGVTIDHCPSCKGSWLEK
                     GEFENIIEALTNEMQSKSFSEYVSSSIEEAKEIITGPESFHSEWKDFSIVLKLLQIRL
                     FVEKPKLIETINSIQKASPIR"
     gene            complement(4921..5289)
                     /locus_tag="AC159U_09730"
     CDS             complement(4921..5289)
                     /locus_tag="AC159U_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337371.1"
                     /translation="MLQIRNIAVALMIVFAVGTTFASLSHAGDEAHKDEIKSMTGDFI
                     CVLPMDANGEVKAVIASGDCSGYSPHVHVFREAGTANVFSIEATPEKLKELEQSSNKT
                     GITLAGKIAGSTRGWVLSVQ"
     gene            complement(5518..5934)
                     /locus_tag="AC159U_09735"
     CDS             complement(5518..5934)
                     /locus_tag="AC159U_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3337372.1"
                     /translation="MSSTQNNKKPKQKAGVVVYRKNENGQTEYLIITSRKFPGTWVFP
                     AGTVEEGETLQQAAARECAEESGYIVLVGEEIKTIEIEQNNKISHFTFYLATITDETD
                     DYEKDRKRKWVSSLELPHVAAEIFSEVAIKAIQIID"
     gene            complement(5955..>6404)
                     /locus_tag="AC159U_09740"
     CDS             complement(5955..>6404)
                     /locus_tag="AC159U_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337373.1"
                     /translation="FKLEYFKNDFPNEDFPYYREIVGEELSKLRERFCNSFGLSHELT
                     PADMNKLTYELAKHYYELDNPITNNNDWKKIILYSGINIKDDDRVLIKWEEYGKIDEM
                     IFKDVCKYLYFIWYEPSEEIGLFDNSCEWIIYFFHYGGVRYYNKSEG"
BASE COUNT         1891 a         1405 c         1210 g         1898 t
ORIGIN      
        1 cttaactgca aggcatgaaa aaccttacat tctaaaacgc gccttgatct actcagcaca
       61 atattcaatt gtcaaagaac ttcatgcacg aagcatgata cacggttcac aatacatgaa
      121 atatgaatcg attgtgcatc gtgaatcctg tatcctgcat catgtaatgg aggtgagcgg
      181 gatcgaaccg ccgacctcct gcttgcaaag caggcgctct cccaagctga gctacacccc
      241 ccagtttata atttccaaat tacagatcca aaatcccaaa tttggggttt ggtgcttgaa
      301 atttggaact gagatactgg tacttaataa tggtgggcct aggtggagtc gaaccaccga
      361 cctcaccctt atcaggggta cgctctagcc tgctgagcta taggcccaga cccaaaaaac
      421 accagacaca aactagaatc ctactcaatc cattgctcat cagtgtcaaa atctggacta
      481 agagcacctg ttaaaaatag cagacacagc agaatccaat atacaccagc aaattaactc
      541 cgtgtaaaag gaggtgatcc agccgcagct tccgctacgg ctaccttgtt acgacttcac
      601 cccaattacc agccctacct tcggagcctg cctccccttc gacaagctcg ggggttagct
      661 cagccacttc gggtagaacc gactttcgtg gtgtgacggg cggtgtgtgc aaggcccggg
      721 aacgtattca ccgcggcgta gctgatccgc gattactagc gattccagct tcatgcaggc
      781 gagttgcagc ctgcaatccg aactgagacg gactttgata ggattagctc cccctttagg
      841 gttcgcaacc cgttgtatcc gccattgtag catgtgtgtg gcccagggta taagggccat
      901 gatgacttga cgtcatcccc accttcctcc aacttgtcgt cggcagtctc tctagagtgc
      961 ccacccgaag tgatggcaac tagtgatagg ggttgcgctc gttaccggac ttaaccggac
     1021 acctcacggc acgagctgac gacagccatg cagcacctgt acaaactgca taaagtaaac
     1081 tttacgccac caaatgcttt cacaccggct taagaaagta tgtcaaaccc tggtaaggtt
     1141 cttcgctttg catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgccaattc
     1201 ctttgagttt cagccttgcg accgtactcc ccaggcggga tgcttaatgc gttagctacg
     1261 gcatagagat ttaaatccct acacctagca tccatcgttt agggcgtgga ctacccgggt
     1321 atctaatccg gttcgctacc cacgctttcg tccatgagtg tcagtactag gccagttagc
     1381 cgccttcgcc gctggtgttc ctcccgatct ctacgcattt caccgctaca ccgggaattc
     1441 cgccaacctc tcctagactc tagtcttgca gtattagatg cattttccgg gttgagcccg
     1501 gagctttcac atctaactta caaaaccacc tgcggaccct ttacgcccag taattccgag
     1561 caacgcttgc cccctccgta ttaccgcggc tgctggcacg gagttagccg gggcttcctt
     1621 ttagggtacc gtcaagtgta tgtgatatta gcacatacac ccatcttccc ctatgacaag
     1681 ggtttacgac ccgaaggcct tcatccccca cgcgacgtcg ctgggtcagg cttgcgccca
     1741 ttgcccaata ttcctcactg ctgcctcccg taggagtcgg ggccgtgtct cagtccccgt
     1801 gtggttggcc acccgctaag gccaactatc cgtcttaggc ttggtgagcc tttacctcac
     1861 caacaacctg atggaacgtg aacccatcct ttggtgatag ctgcaagcag ccacctttta
     1921 ccacaaaatc ctgagaaatc gtggtcttat ccggtattag ctccagtttc ccgaagttat
     1981 cccagtccta agggcaggtt attcacgcat tactcacccg tccgccgctc tactcactcc
     2041 cgaaggagct ttctcgctcg acttgcatgt gttaagcacg ccgccagcgt tcgctctgag
     2101 ccaggatcaa actcttcatg ttaatctgac tatttttaaa actttactac ccgcttcaaa
     2161 tggcgataat gtgtctgcta ttcaattttc aaagatcaat ttaatactta aaagggtggt
     2221 taatataaaa cataaaaaat ctatgtcaag aaatatatta aatatttttt gaccactgta
     2281 aacatttgtt aaaatgtgaa cacccttact tatattgtaa tgtatttata tccaacatac
     2341 aatcaattat ttatcaaaaa acaataatat atactatggt ctgattcttt gttaaaagtc
     2401 aaaaaatttg ttgactgaaa taccttagag gtttattatc tctgcttgta agagatggga
     2461 aaaacaatct gcgttactaa ccaaaaaggc ggggtaggaa aaaccaccac tgtaataaac
     2521 cttggctgct cacttgctgc tgcgcaaaaa aaaactctta taatagatct cgacccgcag
     2581 gcaaatgcta ctagcggtgt tggaatagat aaaaatgagt ctgaaaccac tgtatatgat
     2641 gtattgctgg gaagatcaga cattagaaat tctattaaaa aagttcacca aagcgtccta
     2701 aatgattatc tttttttatg cccttctaat gcagaactta ccggagcgga gattgagctt
     2761 ataggtgttg aaaacaggga atggaaacta aaggaggcga tatctaaaat tgtactagat
     2821 tttgattata tattgataga ctgcccacca tcacttaacc tacttaccat taattcacta
     2881 gctgcggcag attctgtatt aataccagtg cagtgcgaat attatgcgct tgaaggactg
     2941 agccagttaa acaggacaat taaccttata aaagaacgtc taaactcaga tattcaggta
     3001 gaaggttatc tacttactct ttttgacagc agaaacaggt taagcagtgt aatcgtaaat
     3061 gaagtgcgaa cctattttaa agataaagtt ttcgatacca tcgtcccaag aaatgtgagg
     3121 cttgcagagt gcccaagcca tggtttacct attttactct atgatgcaac ctcaaaggga
     3181 gcgcagagtt atatatctct tgcaagggag atcatagaaa acaacaacgg aggctatagg
     3241 agttgagtaa aaaaggagta ctcggaaaag gactggatgc gctcatagtt aaagatgaaa
     3301 agaaaaatag tgagccttcg gataaaaatg caacaaaggt tagcattcat aaaatctttc
     3361 caaatcctga tcagcccaga aaacatttta cagataaaag cattgaagag ctctctgctt
     3421 cgattgcaga aaatggggtt attcagccca ttattgttag aaacaccgat aggggtcttg
     3481 aaataattgc aggggaaaga agatggagag cggcacaaaa ggcaggggta aatgaggtac
     3541 ccgtgattgt taggcaagtt aacgaccaac aatcgcttga attagcatta attgaaaacc
     3601 tgcaaagaga agatttaaat cctgtggaag aagcccgggc gtatgagatg cttattagcc
     3661 agtacgacct cactcatgaa gaaatagcaa agcagattgg caaggacaga actacagtca
     3721 caaacagttt aagactttta aagctatcca caagggcatt ggacgcactg tcatcagggc
     3781 agatcagtca ggggcatgca agagcgcttg ttagtctaga agatgctgca aagataaaca
     3841 tggttttgga gaaaataata tcaggcggac tttcagtaag aaatactgaa gcgcttatta
     3901 aaaaacttct ttccagtaca gaaaaagata caagtgctca accggctgag gaagacttga
     3961 atataaaatt tttcacaaat cagttaaaat ctgccctttc tacaaatgtg cgtatagtta
     4021 ataaaaaagg gaaaggtaaa atagagatcg actataactc ttcggatgat tttgacagga
     4081 tactaaaaaa gcttattcag taatataatt ttaaatatat acagactaaa ccgattaagc
     4141 attgtcacac tatatctagt tatatttatc ttattggact agccttttgt atagaattta
     4201 ttgtttctat aagttttggt ttttctacaa acagcctgat ttgaagcaat tttagaacta
     4261 tagagaaatc tttccattcc gagtgaaatg attcagggcc tgtgataatc tcttttgcct
     4321 cttctatact tgagctaacg tactcagaaa atgattttga ttgcatctca tttgttagag
     4381 cctcaatgat attttcaaat tcaccttttt ccagccaact gcctttacac gaagggcagt
     4441 gatctatcgt cacacctgta tcggcataat taattgaaac caatgcttta ctgcactttg
     4501 gacaaggaag gtcctttgat ttggttttga aaagatgttc atgtttccag atttcaaaat
     4561 ccatccagtt taaattttca tcagtaaggt ctttggcttt gcgtagctca tcactatcaa
     4621 accatatact cttacatttt gcacattcat caacctctaa atctccaaat actttgctgt
     4681 ccagttctgt attacaattg agacaattca tgttagctcc tataagcaat cattttacaa
     4741 tttcattgta tttatttata tatcaatgtc aaaattgata acatgcatgt cataaaatga
     4801 tgtagacagg accgttaata agcttattca gtgatgttca aataaaaata aaaacccggg
     4861 cccaactgta gagtcagacc cggggatctt gctttaattc agattaatag gaattaataa
     4921 ctattgtact gataataccc agcctctggt gctgcctgcg atcttgcctg caagtgtgat
     4981 acccgtttta ttagaagatt gttccagttc ttttaacttt tccggtgtag cttctattga
     5041 gaatacattg gctgtaccag cttctctgaa aacatgtacg tgtggagagt atcctgaaca
     5101 gtcgcctgaa gctataacgg ctttaacctc gccattagca tccataggaa gcacgcagat
     5161 aaaatcgcct gtcatagact taatctcatc cttatgagcc tcatcccctg catgtgataa
     5221 agatgcaaat gtggttccaa ctgcaaaaac aatcattaaa gctactgcta tatttcttat
     5281 ctgtaacata ggtgttaccc ccttatatgg ttttaaatca ttttggtatt tacttaactt
     5341 tttatttttc attttcatct cgttttttag ttatcatatt catgtaaact ttttatttat
     5401 ctatatgtta gttagacatt taagcttaag aaaaggatgc aaagttgtac tgatagattt
     5461 tgaaattaat atgtcagttg gaaaatcgag aaaatactta agcagtttat tcagagacta
     5521 atcaataatt tgaatagcct ttattgccac ttctgaaaag atttcagcag caacatgagg
     5581 aagttcaaga gatgacaccc attttctttt tctgtctttt tcgtagtcat ccgtttcgtc
     5641 tgttattgta gcaagataga aagtaaagtg agaaatttta ttattctgtt ctatttctat
     5701 ggtttttatt tcttcaccta ctagcactat atagccgctc tcttctgcac actctcttgc
     5761 ggcagcttgt tgcagagtct caccttcctc tacagtaccc gccgggaata cccatgtgcc
     5821 gggaaacttt cgtgaggtta tgattaaata ttccgtctgc ccattttcat ttttcctgta
     5881 tacaaccacc ccggcttttt gttttggttt tttgttattt tgtgtgcttg acatgatatg
     5941 accgttgcaa tgttctatcc ttctgattta ttataatatc tcactccacc gtaatggaag
     6001 aagtaaatta tccactcaca actattatca aatagaccta tttcttcaga tggttcatac
     6061 catatgaaat atagatattt acatacatct ttaaaaatca tttcatcaat tttgccatac
     6121 tcttcccatt ttatgagtac tctatcatca tccttaatat taattccact atacaaaatg
     6181 atttttttcc aatcattatt gtttgttatc ggattatcta attcatagta atgctttgca
     6241 agttcatatg taagtttgtt catatcagcc ggtgtcagct cgtgacttaa accaaaagag
     6301 ttacagaatc tctctctgag ttttgacaat tcttcaccaa caatttcgcg ataatacggg
     6361 aaatcctcat tgggaaaatc atttttaaag tattctaatt taaa
//
LOCUS       DBNGSV010000263        13669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1293004, whole genome shotgun sequence.
ACCESSION   DBNGSV010000263 DBNGSV010000000
VERSION     DBNGSV010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1293004"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC159U_09745"
     CDS             <1..410
                     /locus_tag="AC159U_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337374.1"
                     /translation="AFKLSNRLKERLSLRIMLLISQNFYNSKENLTLQNLTDTIGLHQ
                     DDIHESLENLVRNNLLIETNNDPTAYVPAKDLEKISVSDVLSSARSNPVNEEFIESNY
                     LNDQKISEVMNKIEGSIDSSVKELSFKDLISKK"
     gene            complement(400..1266)
                     /locus_tag="AC159U_09750"
     CDS             complement(400..1266)
                     /locus_tag="AC159U_09750"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3337375.1"
                     /translation="MSNLTQRILTAIVGVPILLLVFYFGSIYFLAFILLIVALGSYEF
                     FSLVDNRNLDNKKILGILFCIILPVGAYFGYLYFIFLFTLVSISTLFIHLQKQDLSNT
                     LMGAGTKFLGIVYIGWFLSHAVLIRNVNNSSFPYLDASNIKTTDIGFFWIVFAVACTF
                     LNDTGAYFVGKAKGKTKLAPRLSPGKTVEGTVAGIAMACICGVFINLIFQGPIEYSWA
                     LLMGFVVGVIAVFGDLIESMFKRSVSIKDSGGLLPGHGGVLDRFDSLILVFPVMYYLI
                     LLYYLRDNYLIF"
     gene            1292..2059
                     /gene="purN"
                     /locus_tag="AC159U_09755"
     CDS             1292..2059
                     /gene="purN"
                     /locus_tag="AC159U_09755"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3337376.1"
                     /translation="MNNIKPIFEHSNRPMNIAVFISGSGTNLKALCKYQKALEDSGIV
                     PSANIKCVFTNVPDCKGSKIAKGYDIPVVSLSSKKFFESLYKEPDDADSRKYYDAAVI
                     SLIENVCSPDLIVLAGYRRKLSTLFYEHYKNKIINLYPGDITKEYLITGVQAPIQALK
                     NNETEIKATVYLEQESIRFGIPILQSDNVSLEGYLEDNIDIINKNIREQAEWVIFPHA
                     VYEFIAKGRLGIGNNSTLYLDYDPIGNSGIQYNDINS"
     gene            2129..2437
                     /locus_tag="AC159U_09760"
     CDS             2129..2437
                     /locus_tag="AC159U_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3337377.1"
                     /translation="MGTIHEVAKTDEINEGEAKLVEVDGQEIALFNVGGEYCALANEC
                     SHVGGPLCEGDIEDGKVICPWHGAEFDIKTGDALADPAEGPVESYPVTVDGNTIKIEV
                     "
     gene            complement(2575..3012)
                     /locus_tag="AC159U_09765"
     CDS             complement(2575..3012)
                     /locus_tag="AC159U_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3337378.1"
                     /translation="MSVYLDTSVLVSIYYPESITAKVKTFLKGKKGLTSSTFSIVEFS
                     SAINRKVLRRELSKQSAVEIINRFEDNIDEGYYKICPFSQGDLVAANNFIIDNLGRVS
                     LRTVDALHIAVALRYKCEVFVTADKAQAKSSEILGLKTKFICL"
     gene            complement(3009..3242)
                     /locus_tag="AC159U_09770"
     CDS             complement(3009..3242)
                     /locus_tag="AC159U_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3337379.1"
                     /translation="MSIKEARKQIGYLLDRAKKGEEVILTRRGIPEGKIIAFENNDSG
                     NMGLPDLSDLHESIKVSGKPLSEIVVEEREERY"
     gene            complement(3346..4599)
                     /locus_tag="AC159U_09775"
     CDS             complement(3346..4599)
                     /locus_tag="AC159U_09775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3337380.1"
                     /translation="MESKEFEYLFIGAGILGSSGAMALAENLTAKGKKVSIGVIDIDL
                     SGEHSSTLKNAGGVRATWRNRPNIELCKYSIDFYETIKDEIQFRQLGYYWLHNEQSLA
                     EIEKNLYLYKEYGLNVKTFMPEQIKRFLPFVDNLDGVAGLSISKSAGLIDHYSLREYY
                     RKKAKSLGVEFLDRCYVKDVELDNGCVKNVIAIYVKGTDNSEIIKRYLIEKEINAPHK
                     IVPFSCDVLINTAGAWSPELSKLYGFMDYDIKPRRRQMLVINNPEVDLSAYGMIIDTS
                     DVYFHSEGTSILAGYSNVDEPYGYNFDVSFDTIEEDSPFVKYIWEPLWHRMSKFEKVK
                     LIRGWAGMYGETPDRSGYIGKVAGFENVYECAGHTGRGLMISYGAGHALADLIVDGKF
                     RPELQSAADFSRERPSGDLFEELHL"
     gene            complement(4623..4928)
                     /locus_tag="AC159U_09780"
     CDS             complement(4623..4928)
                     /locus_tag="AC159U_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR3337381.1"
                     /translation="MSKTVMQPIHPGEILLEEFLKPMGISQYILAKDINVDARRINTI
                     VKGKRAISPDTALRLSRYFGTSERFWLNLQTTYDLQVHKDLLADRLKREVKPLSKTG"
     gene            complement(4939..5223)
                     /locus_tag="AC159U_09785"
     CDS             complement(4939..5223)
                     /locus_tag="AC159U_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3337382.1"
                     /translation="MIKDFADSETEKLYYRQKTKKLPAQLLRHALRKLLILDAAETID
                     DLRIPPGNRLEKLKGTRKGQHSIRINNQWRICFVWKDRDAYSVEITDYHS"
     gene            5354..6622
                     /locus_tag="AC159U_09790"
     CDS             5354..6622
                     /locus_tag="AC159U_09790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR3337383.1"
                     /translation="MRDGKPNWLFLDMNAYFASVEQQVCPELRGRPTAVVPVSADTTC
                     CIAASYEAKRHGVKTGTKVAEAKRLCPDINLMPARPALYVEYHNKIVEAVNTVLPVDE
                     VHSVDEMSCRLIGRERNPDYAVSLAKDIKQAIKEQAGDALLCSVGIAPNRFLSKVASN
                     LQKPDGLSVIKGDELPEKLYPLSLSDLPGIGRAMEARLNLYGVTTVEQLCALSKAQCH
                     GIWHSVVGKRWWHLLRGEDMQEVSTKRRTVSHSHVLHPQMRTDEGMYSVMIKLIHKAG
                     ARLRRLGYYTCRVSIHIDYFNPPQPWKDYVKLGQFQDTHTIIQAFLKMWPERPKGKTP
                     LRVSVVLSDLIPTADTSLPLFSDDGRKIKLAETMDKINERYGLDCVYFGTMHTAKESA
                     PMRISFTSIPDLISEGTAHDRSLLAQDTGS"
     gene            complement(6628..7131)
                     /locus_tag="AC159U_09795"
     CDS             complement(6628..7131)
                     /locus_tag="AC159U_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3337384.1"
                     /translation="MSSEKKKKNTADSSKTKVINGLGSRQLVELIESLEGVKLTYDQL
                     YYYENTGLVVPSVRSAQGRGVSRLYSVEDIIILRWIAMLNKKGISIQGFRSVFDFVKN
                     KMPEILKKPQNFVLITDGKSVQFFDKISSRTLDVLKDTGQYLFFFPAGKMAEQSEKAI
                     EKLGSES"
     gene            7262..8089
                     /locus_tag="AC159U_09800"
     CDS             7262..8089
                     /locus_tag="AC159U_09800"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR3337385.1"
                     /translation="MKFGAHVSIAGGIFKAPERASSYGCECFQIFTRSPRGGNPPKLD
                     KETVDKFLNNCEEYNLSDYYVHTPYYINFASENKDIRNSSTRIVTEELERADILNAKY
                     IMTHLGSSKGIERKLAISRVADAAKEILDKTNTKTHLLLENSAGQGETIGEKFEELAE
                     ILDQVNNPELGICIDTAHTFAAGYDIRNKNALNHVLKEFDNIVGIEKIWLIHGNDSKV
                     GLGEKKDRHEHIGQGEIGSEGFESVVNNKHLKSLDMIVETPPENVASDIEILKKMRK"
     gene            8113..8580
                     /locus_tag="AC159U_09805"
     CDS             8113..8580
                     /locus_tag="AC159U_09805"
                     /inference="COORDINATES: protein motif:HMM:NF023036.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HIR3337386.1"
                     /translation="MKSLSIITLLISAIAFSVAVTVGYLHLYNVKQINDIKTQNKSLS
                     EDVQKLQQQLAKSWNKDNKDEHGHHGHKMGNAEEAGDWPKFHEATGVIKFITGQRVIV
                     DQEEMPGFMRAMIMSYEVEKPDQLENFKENDKVKLKLKETATDLTVTEISRAK"
     gene            complement(8597..9169)
                     /locus_tag="AC159U_09810"
     CDS             complement(8597..9169)
                     /locus_tag="AC159U_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR3337387.1"
                     /translation="MKKGNLITAFIALLLVSAACFGPKGFNADQKRDFVANMKTDTLN
                     QLYREKPHTQKLIENAAGYAVFSNVNTQIIFVGGGGGYGVITDNETGNQTYMNMAEGG
                     LALGVGIRDFREIIIFNSPGVLYRFINYGWDLTLAGDAAAKSGDRGGAYGTETDFSSD
                     VVIYQITKNGIALSTSLAGSKYWKDKELNY"
     gene            complement(9345..9839)
                     /locus_tag="AC159U_09815"
     CDS             complement(9345..9839)
                     /locus_tag="AC159U_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337388.1"
                     /translation="MRLIVILFLIFSFTAIDSAHLQEQAEVENSQKPERRKAEYPWYW
                     NRPYENIDNFENSSLGKDRSSITKGAINKSDSVENNVDINKQIEEKIKEDREKQLQYE
                     IEFSSSIGLSVDADGTIIGTQAINEAETIEGTISNKKTITWVDEKGVIHVTNDPTEVY
                     KKSK"
     gene            complement(9873..12941)
                     /locus_tag="AC159U_09820"
     CDS             complement(9873..12941)
                     /locus_tag="AC159U_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3337389.1"
                     /translation="MTKFAIEKNRITIMAVIVILFAGVSAYRTMSRAEDPGFIIRTAQ
                     VTTNWPGASPERVELLITDKLEKSIQEMPEIDFIASTSKTGSSVINVNILETYKDMRP
                     IWDSLRRKVEKAEPLLPDDANKPVVNDEFGDVFGIIVTIIGEGFSYRELKDVADDVRD
                     ELLLIDMAAKVEIYGAQEERIFVEYNNARLSEFGISPVQLRQILQARNIVIPGGDVTT
                     EYEKIVLEPTGNFETVDDLKKTIIEVPGSSNVVYLEDLANIYRSYIDPPNTKMRCGSE
                     RCLGLAVSMREGGNIISLGDDVRQTIDRLVSVYPIGIEFGYLQFQPEPVEKKVKDFTS
                     NLGQAIAIVTLVMLIFLGVRTGLIIASLIPMAMLMALMFMGFFEIGLDQISLAALIIA
                     LGMLVDNGIVMAESVMVQMSSGKSAIDSAVDSANELKVPLLTSSLTTAAAFLPIFLAE
                     STTGEYTASLFKVVTITLLCSWLISLTVIPMLCVKFLKVDPSQQGDNFDSSLYKKYRN
                     LLTWILKNPSKSLIGTAIIFFISLQGFKFVPKIFFPPNERPTFTLEVKLPIGTPIERT
                     DYVVSEIENELANYLVNEDREDGITNWGVFIGQGAPRFQLSFSPEPPSPDYALFIINT
                     SKREIIDSIIPGIEEFCISNFPDIKPTVRPLELGPPSWPPVEVRLSGKDSDILFENVD
                     NVKSKMESTKGTKLIDDDWGARSKKLFVKINQARAYRAGVTSEDIATSLQTSLSGMST
                     TEYREDDKLIPVMLRSVEADRVDIGKLESLNVYVQATGKSLPLKQVADIEVAWQPAVI
                     KRRDRLRTITVESGLESGYTAAEINTTLVPWLKEQSKDWNLGYSWEIGGTDEESGKAN
                     ASISAKLPIAGMIILLLLVGQFNSIRRPVIILITIPLGIIGVVFGLLATNLYFGFMTL
                     LGIISLAGIVINNAIVLLDRINIEITQNGLVPERAVIESAQQRLRPILLTTVTTISGL
                     VPLWLFGGLMFEPMAVAIIFGLLFATALTLGLVPVLYSLFFRLDFKNFTY"
     gene            complement(12960..>13669)
                     /locus_tag="AC159U_09825"
     CDS             complement(12960..>13669)
                     /locus_tag="AC159U_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3337390.1"
                     /translation="SKGELDSARALSESAKAQVDAAQKRLDIDNLQLSYTKLVSPVDG
                     SIAEVPVDINENVNAGTTIVFITSGNEVKVEVAIPEILISKIKKDSMASVRFDSMPEK
                     KFPAIVTEVGVSSTAAATTYPVTVELKRTNEELLPGMAAEVSFEFKNGEQQRIIVPIV
                     AVGEDKSGRYVYILEKISQKEQASFKTGFTKRISVKVGEITPDGIEILEGLKEGDLLV
                     TAGVRRIKDATEVKLLN"
BASE COUNT         4255 a         2868 c         2427 g         4119 t
ORIGIN      
        1 atgcattcaa gcttagcaac agactaaaag aaagattgtc actgagaata atgcttctaa
       61 tttcccaaaa tttttataac agcaaggaaa acctcacact ccaaaacctt acagatacca
      121 ttggtcttca ccaggatgat attcacgaat cacttgaaaa tcttgtaaga aacaacctgc
      181 ttattgaaac gaataatgat ccaactgctt atgtgccggc taaagacctt gaaaagatct
      241 cggtttccga tgtattaagc tctgcaagat caaatcccgt aaatgaagaa tttatagaat
      301 ccaattatct caatgatcag aaaataagtg aagttatgaa taaaattgaa ggttcaatag
      361 actcttctgt aaaagagctc agttttaagg atttaatatc taaaaaataa gataattgtc
      421 tcttagataa tacaataaga tgagataata cataacagga aatacaagta taaggctgtc
      481 aaacctgtcc aataccccgc catgccccgg aagtaaaccg cctgaatctt ttatactaac
      541 acttctttta aacatagatt ctatcaggtc tccaaatact gcaataacac ccactacaaa
      601 ccccatgagc agcgcccagc tgtattctat aggaccctga aaaataaggt taatgaatac
      661 tccgcatata catgccatag ctatgcccgc aactgtgcct tcaactgttt tgcccgggct
      721 taaccttgga gctaattttg tctttccctt ggcttttcct acaaaataag cgcctgtatc
      781 gtttaagaac gtgcaggcca ctgcaaatac tatccagaaa aatccgatgt ctgttgtctt
      841 gatatttgaa gcgtccagat atggaaagct gctgttatta acatttctta taagaaccgc
      901 atggctcaaa aaccagccta tgtacacaat tccaagaaat ttagttccag ctcccataag
      961 tgtatttgaa aggtcctgtt tttgcagatg aataaaaagc gttgatatcg aaacaagtgt
     1021 aaatagaaaa ataaaataga gatagccaaa ataggctcca acaggaagta taatacagaa
     1081 taaaatcccc agtatttttt tattatccag gtttctatta tctacaagac taaagaattc
     1141 ataagagccg agtgcaacta ttaacaaaat gaatgcaaga aaatagatac tgccaaaata
     1201 aaacaccagc agaagaatcg gcacacctac aattgcagtt aaaattcttt gggttaaatt
     1261 actcatctat agctataaac taataaaaat aatgaataat ataaaaccaa tattcgaaca
     1321 ttcaaatcgg ccaatgaata ttgccgtatt tatatccggc tccggcacta atcttaaagc
     1381 attgtgcaag tatcaaaaag cattggaaga ttccgggatt gttccttctg caaatatcaa
     1441 atgcgtgttt acaaatgtac cggattgtaa aggtagtaaa atagctaaag gttacgatat
     1501 tccagtagtt tcacttagtt caaaaaagtt ttttgaatct ctttataaag aaccagatga
     1561 tgctgattca agaaaatatt atgatgcggc tgtaataagt ttgattgaaa atgtatgcag
     1621 tcctgatctg atcgttcttg caggatatag gagaaagctg agcactttgt tttatgaaca
     1681 ctataagaac aaaattataa atctgtaccc aggagatatt acaaaagaat atcttataac
     1741 gggcgttcag gcccccatac aggccctaaa gaataatgaa actgagataa aagctactgt
     1801 ttatctggaa caggagagca ttaggtttgg aattcctata ttacaatcgg acaatgtatc
     1861 actggaaggt tatttagaag ataatattga tatcattaat aaaaatatta gagagcaggc
     1921 cgagtgggta atctttcctc atgctgtata cgaatttata gcaaagggca gacttggtat
     1981 aggtaacaat tctactcttt atctcgacta tgatccaatt ggaaattcag gaattcagta
     2041 taatgatatt aactcataaa aaccgcttga tttatttaaa aatattttat atatctagat
     2101 ttgaagctct tttattaagg aggaacatat gggaactatt catgaagtag ctaaaacaga
     2161 tgagataaac gaaggcgagg ccaagttagt agaggttgat ggacaggaaa ttgcactttt
     2221 taatgttggc ggagagtact gcgcccttgc aaatgaatgc tcacatgttg gaggtccgct
     2281 ttgtgaggga gatattgaag atggtaaagt aatctgcccc tggcacggag cagagtttga
     2341 tattaaaacc ggcgacgccc ttgccgaccc cgccgaaggc cccgtggaat cctatcctgt
     2401 tacagttgat gggaatacga ttaaaattga ggtttaaaat caacttgttt ttttaagttt
     2461 atcctctttt aagtctttaa gataataagt ccttatgtaa ttcaaaatag taggttgtct
     2521 aaagattaag ccagaaattc tcggataact gcaatgaaaa tgaattgaga ataattatag
     2581 gcaaataaac ttagtcttta aaccaagtat ttctgatgat ttagcctggg ctttatccgc
     2641 agttacaaat acttcgcatt tatatctcaa cgctacagca atatgtagag catcaacagt
     2701 tctaagagaa actcttccta aattatctat aatgaaatta tttgctgcaa caagatcacc
     2761 ctgtgaaaaa gggcatatct tataataacc ctcatctatg ttatcctcaa acctgtttat
     2821 aatctcaacc gcgctttgtt ttgagagttc ccttctcaat acttttctat taatcgccga
     2881 actaaactct actatactaa aagtacttga agttaaacct tttttacctt taagaaatgt
     2941 ttttacctta gcagttatag attcaggata gtagatcgaa accaaaacgc tcgtatcgag
     3001 ataaacgctc aatatctttc ttccctttct tctacaacaa tttcactgag tggttttcca
     3061 gagactttaa tagactcatg aagatctgaa agatcaggta gccccatgtt gccgctgtca
     3121 ttattttcaa aagcaatgat cttgccctca ggtatacccc ttcttgtaag gataacttct
     3181 tcccctttct tggctctatc gagcagataa cctatttgtt tccttgcttc tttaatgctc
     3241 actttcattg tacatttaag tgtacataat ctatctatgg attcaaatac ttttggctta
     3301 agatgattat atgttttatt aatagggaga gaaacataac tgcattcaca aatgcagctc
     3361 ctcaaacaaa tcaccagacg gtcgctctct tgaaaaatcg gcagcgcttt gtaactcggg
     3421 tctgaacttt ccgtccacaa ttaaatcagc tagggcatgc cccgctccgt atgatatcat
     3481 aagcccccta cccgtgtgac ctgcacattc gtagacattt tcaaacccgg ccactttacc
     3541 gatatatccc gatctgtccg gagtctcacc atacatccct gcccagcccc ttattagttt
     3601 tactttttca aacttgctca tcctgtgcca aagcggctcc cagatgtatt ttacaaaagg
     3661 gctgtcttct tcaatcgtat cgaagcttac atcaaaatta tatccataag gctcatccac
     3721 attggaatac cccgcaagaa tacttgtacc ctcactatga aaataaacat ctgatgtgtc
     3781 tataatcata ccgtaagcag acaaatcaac ttcggggtta tttatcacaa gcatctgcct
     3841 tcttctgggt ttaatatcat aatccataaa accatatagt ttagatagtt caggcgacca
     3901 cgccccggca gtattaatca agacatcaca gctgaatggc actattttat gaggagcatt
     3961 tatctctttt tctatcagat acctctttat tatttccgaa ttatccgtcc cctttacata
     4021 tattgcaata acgtttttaa cgcagccgtt gtcgagttca acatctttta catagcacct
     4081 gtcaagaaac tccactccaa gtgactttgc tttcttcctg taatactccc tcagagaata
     4141 atgatctata agccccgcgc tttttgatat ggaaagacct gctactccat caaggttatc
     4201 cacaaaagga agaaatctct taatctgctc aggcataaat gtctttacat tcagcccata
     4261 ctctttgtat aagtacaggt tcttttctat ctctgcaaga gactgctcat tatgaagcca
     4321 ataataacca agctgcctga actgtatttc atccttaatc gtctcataaa aatctataga
     4381 atacttacaa agctctatgt ttggcctatt cctccatgta gcccttactc cacctgcatt
     4441 tttaagggta gaagaatgct cacctgaaag atcgatatct atcaccccaa tactaacctt
     4501 ctttcccttc gcggttagat tctctgcaag cgccatagca ccggacgagc ctaaaattcc
     4561 ggcccctata aaaagatatt caaattcttt agattccatc gataacccaa cctttttttt
     4621 aatcaccctg tttttgatag aggtttaact tctcttttaa gcctgtcggc cagcaggtct
     4681 ttatgtactt gcagatcata ggtcgtctga aggttaagcc agaatctctc tgatgtacca
     4741 aaatatctgg atagccgcag tgcagtatca ggcgatattg cacgcttgcc ttttactatc
     4801 gtattaatac gccgtgcatc tacatttata tcttttgcaa gaatatactg gctaataccc
     4861 attggtttta aaaactcttc cagcaagatt tcacctggat gaattggttg cattactgtt
     4921 ttgctcatat ttatctcctt aagagtgata atcagttatt tcaaccgagt aagcatctct
     4981 gtccttccac acaaagcata ttctccactg attatttatt cttatactgt gctgaccttt
     5041 ccttgttccc tttaatttct ccagcctgtt tcccggagga attcgcaaat catcaatggt
     5101 ttcagccgca tccagtatca atagttttct aagtgcatga cgaagtaact gagctggcaa
     5161 tttttttgtc ttttgccgat aatatagttt ttcagtttct gaatcagcaa aatcctttat
     5221 cactcaaata taatagcgtg tcacgccatt attgtcaacc ttgattttga attaacattg
     5281 cttgtcttat atacattaaa acaaacccca atagacactt tttgggatat ggtttattat
     5341 attgtgtggg tatatgagag atgggaagcc gaactggctt tttctagata tgaacgccta
     5401 ctttgcttct gtggagcagc aggtctgtcc ggagctaaga ggccgtccga cagccgtggt
     5461 accggtgagt gcagatacca cctgctgtat tgctgcaagc tacgaagcca aaaggcatgg
     5521 tgttaaaacc ggcacaaagg tagcagaggc aaaaaggctc tgccccgata tcaaccttat
     5581 gccggcaagg cccgcgcttt atgtcgaata tcataataag attgtagaag ccgtaaatac
     5641 ggttcttccc gtagatgagg ttcactctgt agacgagatg tcatgcaggc ttattggtag
     5701 agagcgcaat cctgactatg ccgtttcgct tgcaaaagat ataaagcagg ccataaagga
     5761 acaggcagga gatgcccttc tatgctctgt agggattgct cccaaccggt ttctttccaa
     5821 ggtagcaagc aacctgcaaa agccagacgg ccttagcgtt ataaaagggg atgagcttcc
     5881 cgaaaaactc taccctcttt cgctctccga ccttccagga attggaagag caatggaagc
     5941 acgtcttaat ctctatggtg taacaacagt agaacagctc tgcgccctat ccaaagcgca
     6001 gtgccacggc atatggcaca gcgtagtagg aaagcggtgg tggcaccttc ttaggggaga
     6061 agacatgcag gaggtttcca caaaaagaag aactgtgagc cactcccatg tgctgcatcc
     6121 gcagatgcgc acagatgaag ggatgtactc tgtaatgata aaactaatcc acaaggcagg
     6181 ggcaaggctg aggagacttg gttactacac ctgccgcgtg agtattcata ttgactattt
     6241 taatccgcct caaccctgga aagattatgt gaagctcggc cagttccagg acactcacac
     6301 cataatacag gcatttctta aaatgtggcc agaaagaccc aaaggcaaaa cccctcttcg
     6361 cgtatctgta gttttgtccg atttaatccc cacagccgac acaagccttc cgcttttctc
     6421 cgatgacggc agaaaaataa aacttgcaga gaccatggac aaaataaacg aacgctacgg
     6481 gctggattgt gtttactttg gcacaatgca tacagcaaaa gaatcagcac cgatgcgcat
     6541 atcatttaca tcaatacccg acctcatatc cgaagggact gcgcatgacc ggtcgcttct
     6601 ggctcaggac actggaagtt agaacagtta agattccgaa ccaagttttt caatcgcctt
     6661 ttcactctgc tctgccatct tgccggcagg aaagaagaag aggtattgtc ctgtatcttt
     6721 tagaacgtct aaagtacggg atgagatttt atcaaagaac tggactgatt tgccgtctgt
     6781 aataagaaca aagttctgag gctttttaag aatttcaggc attttattct taacaaaatc
     6841 aaagacgctg cgaaagcctt gtatagatat gccttttttg ttaagcattg ctatccaacg
     6901 gagtataata atgtcttcta cagaatacag gcgggatacc ccccttccct gcgcacttct
     6961 tacgcttggc acaaccagcc ccgtgttttc ataatagtag agctgatcgt atgtaagctt
     7021 tactccctcg agcgattcaa taagttcgac tagctgcctg cttccaagac cgtttattac
     7081 cttagttttg gaggagtctg cagtattttt cttctttttt tctgaagaca tgtatctaat
     7141 tggtacctaa tttaatatta tagtgattta tacccaaaaa ataaatagaa ttatcagatg
     7201 attaagtgca aattcgaaat agactgatat aatgaaccag atttattaaa tcgagataaa
     7261 tatgaaattt ggagcccacg tatcaatagc aggaggaata ttcaaagcac ctgaacgcgc
     7321 aagcagctat ggctgcgaat gctttcagat ctttactcgc tccccaaggg gcggcaatcc
     7381 tccaaagctg gataaagaaa ctgttgataa attcttaaat aactgtgaag agtacaatct
     7441 ttctgactac tatgttcata ctccttacta tattaacttt gcttctgaaa ataaagacat
     7501 aagaaattca tccacaagaa tagtaacaga agagctggaa agggctgata tattaaatgc
     7561 taaatatata atgacacacc ttggaagctc aaaaggtata gagaggaaat tagccatatc
     7621 aagagttgct gatgctgcaa aagaaatatt ggataaaaca aataccaaga ctcatctgct
     7681 tttagaaaac tctgccggac agggtgagac aataggagag aagtttgaag aacttgcaga
     7741 aatactcgat caggtaaata atccagagct cggaatatgc attgataccg cccacacatt
     7801 tgctgcagga tatgatatta gaaacaaaaa tgcgctaaat catgttctaa aagaatttga
     7861 taatattgtt ggaatagaaa aaatatggct tattcatggc aatgactcaa aagtaggact
     7921 tggagaaaaa aaagaccgtc atgagcatat tggtcaaggt gaaattggct cagaaggatt
     7981 tgaatctgta gtgaataata agcatcttaa atctctggat atgattgtcg aaacaccccc
     8041 ggaaaatgta gcatcagata ttgagatatt aaaaaaaatg agaaaataaa atcaagggag
     8101 aattaaaaat aaatgaaatc actttctata atcacattat taatttcagc aattgcattt
     8161 tcagtagccg tcactgtggg ttatctacat ctttataatg ttaaacagat aaacgacata
     8221 aaaactcaga ataagtcctt atctgaagat gttcaaaaac ttcagcagca gcttgcaaaa
     8281 tcatggaaca aagacaataa agacgagcat ggacatcatg gccataaaat gggaaatgct
     8341 gaagaagcag gagactggcc aaaatttcac gaagccacag gggttataaa atttattacc
     8401 gggcaacgcg tcattgtaga ccaggaagaa atgcccggtt tcatgagagc catgataatg
     8461 agttatgagg ttgaaaaacc agatcagctg gaaaacttta aagaaaatga caaagtaaaa
     8521 ttaaaactca aagaaaccgc tacagatcta acagttaccg aaataagcag ggctaagtaa
     8581 cctattttat ttgagtttag tagttaagct ctttatcttt ccagtatttt gatccggcca
     8641 ggcttgtgct gagagcaatg ccgttttttg tgatctgata tatgactaca tcggatgaaa
     8701 aatcagtttc tgttccataa gctccacccc tgtctccgga ttttgcagct gcgtcaccgg
     8761 caagcgtcaa atcccatcca taattaataa acctgtatag aactccgggg ctgttaaata
     8821 ttattatttc tctaaaatcc ctaatgccta ctccaagagc aagtccaccc tcagccatat
     8881 tcatataagt ctggtttccg gtttcattat cagtaataac tccataacct cccccgcccc
     8941 ccacaaagat tatctgggtg ttaacattac taaaaaccgc gtaacctgct gcattttcta
     9001 taagtttttg tgtatgaggt ttttccctgt aaagttggtt gagcgtatca gttttcatat
     9061 tggctacaaa atctcttttt tgatcagcat taaaaccttt tggtccaaaa caggctgctg
     9121 aaactaataa gagtgcaata aatgcagtaa taagatttcc ttttttcata gttccctccg
     9181 gttataaaga actttttact caatatctgg tatacaacta taaaacaaat acaatactta
     9241 gcaataatat agcatgttag aacgggataa caacaaaata tttagtcaaa aatatgttgg
     9301 caataaagca atcgatacta gtataataat gtgaagttta tcgtttattt agacttttta
     9361 taaacctcgg taggatcgtt tgtaacatga ataactcctt tttcatcaac ccatgtaatt
     9421 gtctttttat tactaatagt accttctatg gtttcagcct cgtttatagc ctgagtacca
     9481 attatagtgc catcagcatc aacactaaga ccaattgagc tgctaaactc tatctcatac
     9541 tggagttgtt tttctctgtc ctctttgatc ttttcttcta tttgtttatt aatgtctacg
     9601 ttgttttcta cagaatcact cttatttatt gctccctttg taatagagct tctatcttta
     9661 cctaaagatg agttttcaaa attatcaata ttttcataag gcctgttcca gtaccagggg
     9721 tattcagctt ttctacgctc tggtttttga gaattttcta cttcagcctg ttcttgcaaa
     9781 tgagcagagt ctatggctgt aaaagaaaat attaagaaca gaataactat taacctcata
     9841 atcatttctc cgatatttta ttaattataa gtttaatatg taaaattctt aaaatccagt
     9901 ctgaaaaata acgaatatag aactggtaca agccccaggg taagcgctgt ggcaaaaaga
     9961 agtccgaaga ttattgcaac agccataggt tcaaacatga ggccgccaaa aagccagagt
    10021 ggaacaagtc ctgagattgt tgtaacggtg gttagtaata ttggtcttag cctttgctga
    10081 gcagactcta ttacagccct ttcaggcaca agaccatttt gggtaatctc tatatttatc
    10141 ctgtccagta gaacaatagc gttattaatt acaattccgg caagagaaat tatgccaaga
    10201 agagtcataa atccaaaata caggtttgtt gccaaaagtc cgaacactac tccaataatt
    10261 cctaggggta tggttataag aataatcacc ggtcttctga tagagttaaa ctgtccaacc
    10321 agtaaaagta aaataatcat tcctgcaata ggaagttttg cgcttattga cgcgttggct
    10381 tttcctgatt cttcatcggt cccaccaatt tcccatgagt atccaagatt ccagtccttg
    10441 gactgctctt ttaaccacgg aacaagcgta gtatttatct cagctgccgt atagcctgat
    10501 tccaatccgg attctacagt tatagttcta agcctgtccc ttcgttttat tacagccggc
    10561 tgccaggcaa cctctatatc ggcaacctgt tttaagggaa gggattttcc tgtagcctgc
    10621 acatagacat tcaggctctc cagttttcct atatcgactc tatctgcttc aacagagcgc
    10681 agcataacgg gtattagctt atcatcctct ctatactccg tagtgctcat accgcttagt
    10741 gatgtttgca aagatgttgc tatatcctca ctcgttacac cagctctata ggcccttgcc
    10801 tgattgatct taacaaagag ctttttagac cttgctcccc agtcgtcatc aatcaacttt
    10861 gtacctttgg tgctttccat tttactttta acattgtcta cattttcaaa caaaatatca
    10921 ctgtcctttc ccgagagcct cacttctaca ggcggccatg aaggtgggcc tagttcaagg
    10981 ggacgaacag tgggtttaat gtcaggaaaa tttgaaatac aaaactcttc aattccggga
    11041 ataatgctgt ctatgatttc cctcttggaa gtattaataa taaagagtgc ataatcaggg
    11101 cttggcggct ccgggctaaa cgaaagctga aaccttggcg ctccctgccc tataaacact
    11161 ccccaatttg ttataccatc ctctctatcc tcatttacta aataatttgc cagttcattt
    11221 tctatctctg ataccacata atcagttctc tcaattggcg tgccaatggg aagtttaact
    11281 tcaagagtaa aggttggtct ttcatttgga ggaaagaaga ttttcggaac aaatttaaat
    11341 ccctgaaggg atataaaaaa tataattgca gttccaatca gagacttaga tggattttta
    11401 agaatccagg ttagaagatt tctgtatttt ttatataaac tgctatcaaa attatcgcct
    11461 tgctgtgagg gatcgacttt taaaaacttt acacaaagca taggaataac ggtaagcgaa
    11521 ataagccaag aacagagtaa cgttatagtt actaccttaa aaagcgatgc tgtgtattcc
    11581 ccagttgtag attcagcaag aaatattgga agaaaggcag cagcagttgt aagtgaagaa
    11641 gtaagcagtg gaacttttaa ttcatttgca gagtccactg cagagtcaat cgcagatttt
    11701 ccactactca tctgcaccat taccgattca gccataacaa tgccgttatc tacaagcatt
    11761 ccaagagcaa ttataagggc agcaagtgaa atctgatcaa gacctatttc aaaaaagccc
    11821 ataaacataa gcgccataag catggccatg ggtattaggc ttgcaataat aagccctgtt
    11881 cttacaccaa gaaaaatcag cataacaagc gtaacaattg ctatggcctg accgagatta
    11941 cttgtaaaat ccttaacttt cttttctacc ggctctggct ggaactgcag ataaccaaac
    12001 tcaataccga ttggataaac tgataccaaa cggtctattg tttgccttac atcatcacca
    12061 agcgaaatta tattaccgcc ttctctcata gaaactgcaa gcccaagaca tctttcagag
    12121 ccgcatctca tcttggtatt tggcggatca atgtatgacc tgtatatatt tgcaaggtct
    12181 tcaaggtaaa ccacattact cgatccggga acttcaatta ttgttttttt aagatcgtct
    12241 acagtctcaa agtttccagt tggctcaagt actattttct catactctgt tgtaacgtca
    12301 ccccccggaa taacaatgtt ccttgcctgc aggatctgcc ttaactgtac aggagatatg
    12361 ccaaactctg agagtcttgc attgttatac tcaacaaata tcctttcttc ctgtgcaccg
    12421 taaatttcta cttttgctgc catatcgatt aacagcagct catcacgtac atcatcggca
    12481 acatctttta attccctgta agaaaatcct tccccaataa tagtgacaat tattccgaac
    12541 acatctccaa actcatcatt aacaaccggt ttattagcat catcgggcag aagaggctcg
    12601 gctttttcca cctttctgcg caggctgtcc caaattggac gcatatcctt ataagtttct
    12661 aatatgttta cattaataac tgatgaacct gttttggatg ttgacgcgat aaaatcgatt
    12721 tcaggcattt cctgtatcga tttttctagt ttatcggtga taagaagctc aaccctctca
    12781 gggcttgccc ccggccagtt ggtagtaacc tgtgcagttc taattataaa tcccgggtcc
    12841 tcggctctag acatagtcct gtatgccgag acacccgcaa acaaaataac aataacagcc
    12901 attatcgtta ttctattttt ttctatagca aatttagtca aatcagattg cctgtgaatt
    12961 tagttcagta attttacttc tgttgcatcc ttaatgcgtc ttacccctgc tgtaaccagt
    13021 aggtctccct ctttaagacc ttctaatatc tctattccat cgggtgtaat ctctccgact
    13081 tttacactta ttcgtttagt aaaacccgtt ttaaaagatg cctgttcttt ctgagatatt
    13141 ttttcaagta tataaacata tcttccgctt ttatcttcac ccacagccac gattggaaca
    13201 ataatccttt gttgttctcc gtttttaaac tcaaatgaca cttcagcagc cataccgggc
    13261 aaaagctctt catttgttct tttaagctcc acggttaccg gatatgtagt agctgctgct
    13321 gtggaagata caccaacctc tgttactatt gcagggaatt tcttctcagg catggagtcg
    13381 aatctaacac ttgccatact gtccttttta attttagaaa taagaatttc gggtatagcc
    13441 acttcaactt taacttcgtt accagaagta ataaacacta ttgtagtacc ggcgtttaca
    13501 ttctcattaa tatcaacggg tacttctgca attgatccgt ctacaggaga gacgagtttt
    13561 gtataactta gctgcagatt atctatgtcc agcctttttt gagcagcatc aacctgcgcc
    13621 tttgcagatt ctgaaagcgc tcttgcagaa tcaagttctc cctttgatg
//
LOCUS       DBNGSV010000264         5113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_462109, whole genome shotgun sequence.
ACCESSION   DBNGSV010000264 DBNGSV010000000
VERSION     DBNGSV010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_462109"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC159U_09830"
     CDS             <1..843
                     /locus_tag="AC159U_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3337391.1"
                     /translation="MKKLNLFILLLLTFFMFAFGLTDIVRKIDLLRNIFYPEVYERKG
                     LGNDTKGGKFDHLAYNSVLKMAVNDDGYVDYIAISNNPHSLDEYIKKLANADVKSLSR
                     FEHFALMVNAYNAFTLKLITEYPGIDSIKDISSSKRWDDKRWRIGDAVYSLNEIEHDV
                     LRKQYGEPLVHFALVCASKSCPKLRNEPYIGKDLAFQLNDQALHFFSQPINFRWDEEK
                     NTVFLSELLDWFRHDFAVDEKGVLDYPLHYIDTRSEAQIKANKDSLKIKYIPYDWSIN
                     GNWN"
     gene            856..1575
                     /locus_tag="AC159U_09835"
     CDS             856..1575
                     /locus_tag="AC159U_09835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3337392.1"
                     /translation="MIELSIIIPALNEAGYITKTLNSVLDNRCYENKPEIILVDSGSF
                     DKTVELAKPIVDKVIELEIKASGKWEALNTGAEASTGEVLLFLDADTIVPKDYDKHIE
                     TALSDKNIIGGAFEFSLDGKEFGLRVVELINRVRYRIRHSFYGDQGVFVTSDKFFNSG
                     MFPKRRLFETSELCKRLRKYGNLKLIKKHALTSPRRFIDGGIYKVLLNDTKLWFLNNL
                     GLLPDDKRADVYWKENERRGK"
     gene            1830..2978
                     /locus_tag="AC159U_09840"
     CDS             1830..2978
                     /locus_tag="AC159U_09840"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR3337393.1"
                     /translation="MIIGIPNEILEGEKRVALAPSHVPSLQKLGFIVHIEAGAGLSAG
                     FTDKMYEDEGVKIISNQDELISGSEIILHVRGLGTSNDPNAGYTQKLKNKILIGLLDP
                     FNNPEVVKSLASNGVQAFAMELIPRITRAQSMDVLSSQANIAGYKAALIAADSLPKMF
                     PMMMTSAGTATPARVFIIGAGVAGLQAIATCNRLGAIVSSYDIRPAVKEQVQSLGAKF
                     VEFDLDTGESEDKGGYAKEMDDAFYKKQQQMMRDVIAEHDVVITTAAIPGKKAPILVT
                     SDMVSAMKHGSVIVDLAAESGGNCEVTKPGETIDVGGVTVIGPKNVPSMVPFHASQMY
                     SKNLANFLGLMVDKEGKFEINKEDEVISGALVTDGGAVVHETVKQAIG"
     gene            3004..3282
                     /locus_tag="AC159U_09845"
     CDS             3004..3282
                     /locus_tag="AC159U_09845"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR3337394.1"
                     /translation="MDFAIVFTIFVLATFVGFEVITKVPPTLHTPLMSGSNAISGIAI
                     IGALLSAGMQQSTITTILGTLAVIFATINVVGGFMVTNRMLEMFKKKE"
     gene            3289..4683
                     /locus_tag="AC159U_09850"
     CDS             3289..4683
                     /locus_tag="AC159U_09850"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR3337395.1"
                     /translation="MSPTLVNLAYLVAAALFIMGLKGLSHPRTAVRGNMLGAIGMLIA
                     IVVTLMDKNIIGYQYIIAGLAVGAVIGAVFAIKVEMTEMPELVALYNGFGGAASVFVA
                     GAALYEVIAITSIDPATQATVAIAASGLIGAVTLFGSLVAFGKLKGILGDAPFIYSGE
                     QIVKVVLAVIVLALSYLLVTNPENTIVFWGLVVVASVLGVLLVIAIGGADMPVVIALL
                     NSYSGLAASATGFIINNTILVIAGTLVGASGIILTRIMCKAMNRSLTNVLFGGFGAEV
                     TSGPSADDVYEGKVKATSAEEVAMLLEGCKRVCIVPGYGLAVAQAQHAVRDLTNLLED
                     RGVEVLFGIHPVAGRMPGHMNVLLAEADIPYDNLKEMDEMNPMFNQVDLSIVIGANDV
                     VNPVARTDPQSPIAGMPILDVDYSKTVVIIKRSLSPGFAGIPNPLFALDNSLMLFGDA
                     KGMVDGMVKALKEG"
BASE COUNT         1561 a          977 c         1187 g         1388 t
ORIGIN      
        1 atgaaaaaat taaatttatt tatcctactt ttgctaacct tctttatgtt tgcatttgga
       61 ctcactgata tcgttagaaa aattgattta ttaagaaata tcttctatcc ggaggtttat
      121 gaaagaaaag ggcttggaaa tgatactaaa ggcggtaaat ttgaccattt ggcctataat
      181 tcggtactaa aaatggccgt aaacgatgat ggatacgtag attatatagc aatatccaat
      241 aacccccata gccttgatga atatataaaa aagcttgcaa atgctgatgt aaagagcctt
      301 tcgaggtttg aacactttgc ccttatggta aatgcctata atgcgtttac actaaaactt
      361 ataaccgaat atccgggtat tgattccata aaggatataa gctcctccaa aaggtgggat
      421 gacaagaggt ggcggattgg agatgctgtt tatagcctga atgaaataga gcatgatgtg
      481 cttagaaagc agtacggcga accgcttgtt cactttgccc ttgtttgtgc atcaaagagc
      541 tgtccaaagc tgcggaatga accatatatt ggcaaggatc ttgcttttca gctaaacgat
      601 caggcactgc atttcttctc tcaacccata aatttcaggt gggatgaaga aaaaaataca
      661 gtgttccttt ctgaactact ggactggttc aggcacgact ttgctgtgga tgaaaaaggg
      721 gtgctggatt atcccctgca ttatatagat accagatcag aagctcagat taaggcaaat
      781 aaagatagtc ttaaaattaa atatatcccc tacgactggt ctattaacgg caactggaac
      841 tgattttcta taactgtgat tgaattatcc ataatcatcc ccgctctaaa cgaggccggg
      901 tatatcacaa aaactctaaa ctcagtactg gataacaggt gttatgaaaa caagcctgaa
      961 attattctgg tagatagcgg ttcttttgat aagactgttg aattagctaa accaatagta
     1021 gataaggtta tagaattgga aattaaggca agcggaaagt gggaggcttt gaataccgga
     1081 gccgaggcaa gcacggggga agttctgctt tttctcgatg ccgatacgat tgttccaaaa
     1141 gactatgata aacacataga gactgctttg tctgataaga acataatagg cggggctttt
     1201 gagtttagtc ttgatggaaa agagtttgga cttagggtag tggagcttat aaacagggtt
     1261 agataccgga tccgacacag cttctacggt gatcagggtg ttttcgttac aagcgataaa
     1321 ttttttaatt caggaatgtt tcccaagaga cgactgtttg aaacctcgga gctttgcaaa
     1381 aggcttagga aatacggaaa cctcaaactt attaaaaaac acgccctgac ttctccacga
     1441 agattcatag acggtggaat atacaaagtc ctgctaaacg atacaaaact ctggttttta
     1501 aataaccttg gtctccttcc tgatgataaa agagcagatg tttattggaa ggagaatgag
     1561 aggcggggga aataggttag atagtaaaat acaactatca aattacaact attacatatt
     1621 aatattccgt aaatatccaa ttctcaaact tccaagaaca ttaattaaat ttctccattg
     1681 ccaaatattc tcattaatgg tataaataca accaatttta tatgatttat ttctaagggg
     1741 tgggcaaaaa gtagtaaaaa cagaggtagg accggcgata aatctttgta aaatactaat
     1801 aatttaatta atcaacaact taaaaaatta tgataatagg tattccaaac gagattctag
     1861 aaggtgaaaa aagggtagct cttgctccaa gtcatgtgcc ttcgcttcaa aaactgggtt
     1921 ttattgtaca catagaagca ggtgcagggc tttcggcagg ttttaccgac aagatgtacg
     1981 aagacgaggg tgtgaagatc atcagcaatc aggatgaact tatctcggga tctgaaatca
     2041 ttcttcatgt aagaggcctt ggcacctcaa acgacccaaa cgcaggctac acccaaaaac
     2101 taaaaaataa gatactcata ggactactcg atccttttaa taatcctgaa gttgtaaaat
     2161 cacttgcctc aaacggcgtt caggcctttg ccatggagct tattccaagg atcacaaggg
     2221 cacagagtat ggacgtactg tcgtctcagg caaatatagc aggatacaag gcggcgctaa
     2281 tagcagcaga ctccctgcca aagatgttcc cgatgatgat gacctcggcg ggtacggcca
     2341 ctcctgcaag ggtgtttata ataggcgctg gtgtagcagg gcttcaggcc attgccacat
     2401 gcaacaggct tggcgctata gtaagctcat atgacattcg ccctgccgta aaagaacagg
     2461 tgcagagcct tggggcaaaa tttgtagagt ttgatctgga cacaggcgag tctgaggaca
     2521 agggcggata cgccaaggaa atggacgatg cattttataa aaagcagcag cagatgatga
     2581 gagacgtaat agccgagcat gatgtggtaa tcaccacagc cgctattccg ggaaaaaagg
     2641 ccccgatact tgttacaagt gatatggtct cggcaatgaa gcacggctct gtaatagttg
     2701 accttgctgc agaatcgggc ggaaactgcg aagttaccaa accaggcgaa acgattgatg
     2761 tgggcggggt aaccgtaata ggtcccaaga acgtaccatc catggtgccg tttcatgcaa
     2821 gccagatgta ctctaaaaac cttgccaatt tcctgggatt gatggtagac aaagaaggaa
     2881 aatttgaaat taataaagaa gatgaagtca taagcggcgc acttgttaca gacggcggtg
     2941 ccgtggttca tgaaaccgta aaacaggcta tagggtaaaa aataatttac agaggatagc
     3001 aatatggact ttgcaatagt atttacaata tttgttcttg ccacttttgt gggttttgag
     3061 gttattacaa aagtgccccc aaccctgcat actcctctaa tgtcaggctc aaacgcaatc
     3121 tcgggtattg caattattgg agcgcttctt tcagcaggaa tgcagcagtc aacgataacc
     3181 actattttag ggactctggc agtgatattt gccaccataa acgtagtggg tggttttatg
     3241 gttacaaaca gaatgttaga aatgtttaag aaaaaggagt aataccaagt gtcaccaaca
     3301 ttagtcaact tagcttatct agtagcagca gcactattta taatgggcct gaaaggcctt
     3361 tcgcatccaa ggaccgcagt gcgtggaaac atgcttggcg caattggtat gctcatagcc
     3421 attgtcgtta cacttatgga taaaaacata atcggttatc agtacattat tgcggggctt
     3481 gcagtaggag ccgtaatagg agcagtattt gcaattaagg tagagatgac agagatgccg
     3541 gagcttgtgg cgctttataa cggttttggc ggagcggctt cggtgtttgt agcgggagcg
     3601 gcgctctatg aagttattgc catcacaagc atagaccctg ccactcaggc cacagtggct
     3661 atagcggctt cgggtcttat tggagcagtt acgctttttg gaagcctcgt ggcgtttgga
     3721 aaactaaagg gcattcttgg tgacgccccg tttatatact ccggtgagca gattgtaaaa
     3781 gtagtgctgg cagtaattgt gcttgctctt tcttacctgc ttgttactaa tccggaaaac
     3841 acaattgtat tctggggtct tgtagtggtt gcatcggtac ttggagtgct ccttgttata
     3901 gccataggcg gagcggacat gccggttgta atagcacttt taaactctta ttccggtctt
     3961 gccgcatcag caaccgggtt tattataaac aacacaatcc ttgttatcgc aggaacgcta
     4021 gtcggagctt cgggaattat tcttacaaga attatgtgta aggccatgaa ccgttctctt
     4081 acaaatgtgc tcttcggagg ctttggcgca gaggtcacaa gcgggccgag tgcagacgat
     4141 gtttacgaag gtaaagttaa ggccacatct gccgaagagg tagcaatgct gcttgaaggc
     4201 tgtaagagag tgtgcattgt gccaggttac gggcttgcag ttgctcaggc gcagcatgcg
     4261 gtaagggacc ttacaaatct gcttgaagac agaggggttg aagtactttt tggtattcat
     4321 cccgtggcag gaaggatgcc cgggcatatg aacgtgctcc ttgccgaggc cgatattccc
     4381 tatgataacc taaaagagat ggacgagatg aaccccatgt ttaatcaggt tgatctctca
     4441 atcgtaatcg gtgcaaacga cgtggttaac cccgttgcaa gaaccgaccc acagagtcca
     4501 atcgccggaa tgcctattct ggacgtagac tactcaaaga cggttgtaat cataaaaaga
     4561 agtctaagcc cgggttttgc aggtattcca aacccgctgt ttgctctcga taattctctt
     4621 atgctctttg gtgatgcaaa gggaatggta gacggcatgg tcaaggctct taaagaaggg
     4681 taggtcgtat tctcccataa tttccatatt caatcagaca gtattggtgc gttctatcca
     4741 ccacaacggt catcgtactt gcaattctgt cattcttgag gacggggctt ttttgaataa
     4801 aagaagttcg taagacctta aatatttata aaaatcatag tagctgaaga atctaaaaac
     4861 tagatgcttc acattcgttc agcatgacgt ttgtgtgttt tcctaacaca ctgagcccag
     4921 ttccttcgac tctgctcgtt gcaggaagaa gtctgagtta agataaacta accacaactg
     4981 tcattgctgt agatgtcaca taattcgcag acaaaataca taagtaacta agtagagggg
     5041 gtattttgtt aagatgcgaa taaacaggag tgatttaaaa cttaagaaaa actatataca
     5101 gaagtacagg ttt
//
LOCUS       DBNGSV010000265         7262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_323182, whole genome shotgun sequence.
ACCESSION   DBNGSV010000265 DBNGSV010000000
VERSION     DBNGSV010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_323182"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC159U_09855"
     CDS             complement(<1..204)
                     /locus_tag="AC159U_09855"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR3337396.1"
                     /translation="MDRKKVLFVCTHNSARSQMAEGILNSFHSDRLHAYSTGSDPTQI
                     NPYSIKVLKEIGVDISGNYSKGFS"
     gene            complement(231..1085)
                     /gene="pstB"
                     /locus_tag="AC159U_09860"
     CDS             complement(231..1085)
                     /gene="pstB"
                     /locus_tag="AC159U_09860"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR3337397.1"
                     /translation="MEENQLEQSTDNKQISTGPADSEQLETFIKQDIEDPLVVVKNLC
                     LSYGDKQALYDINLLIPKKKVTAFIGPSGCGKSTLLRCFNRLNDLIDNVTISGAIEIN
                     GRDINDPNLELTELRKKVGMVFQKSNPFPKTIYENVAYGARIAGINEKDELDHIVEKS
                     LKGAALWEEVHDRLDESALGMSGGQQQRLCIARAIAVEPEVLLMDEPCSALDPVATTR
                     IEDLITELKENYTIVIVTHNMQQASRVSDLTAFMYLGKMIEYDETQNIFINPTIPQTQ
                     DYVTGKFG"
     gene            complement(1188..2807)
                     /gene="pstA"
                     /locus_tag="AC159U_09865"
     CDS             complement(1188..2807)
                     /gene="pstA"
                     /locus_tag="AC159U_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR3337398.1"
                     /translation="MKNFFKSGDPFVWLTGTALMFSLIAIFGLLFIIASKGLGFFWPS
                     DIAQVELVDGKKYLGQISGREKIRVPNSKEKSKKTRIQLKVGNRDIYGFDFKWIDDDQ
                     IKNISYPENAFLIERLEWGNMYGFVTGIDEGSNIVSSTTEQSITELAEILPRTTAKYK
                     EIKEIEKHDIGDINYEMEAIRLKKKNLNQSDPEDIKTLDKLNKQFAEQEEKFKIEEKK
                     LTALYTELDQYKVKLETVDGRTKEFSAGLIVRAYRPNSMGTFSKLMLYMDKFWEFLSA
                     DPREANTEGGVFPAIFGTVMMVIIMSIVVLPFGVLAALYLREYARQGTMVRIVRICVN
                     NLAGVPSIVFGVFAVGFFIYGIGGAIDQILYSDQLPAPTYGTGGILWASLTLALLTVP
                     VVIVATEEGLAAVPRNIREGSLALGATKFETMWKVVLPSAAPAIMTGLILAIARATGE
                     VAPLMITGVVKLAPDLPIDGYFPFVHLERKFMHLGFHIYDVGFQSPNVEAAKPMVYTT
                     TFLLIVIVILLNLAAIVIRNRLKKKYSSSQI"
     gene            complement(2820..5144)
                     /locus_tag="AC159U_09870"
     CDS             complement(2820..5144)
                     /locus_tag="AC159U_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3337399.1"
                     /translation="MEVITESEKKINSQAASKEKAIRKRRLADKIATVVVTSGGIAII
                     LSIIAILLFIGIEVVPLWEKVKTTVASNFDLNENKSIFILPAVPPSENSSLALTPKPD
                     IVAIGVEEFREIAYLITDNGYVHFIDLANGKNLKKIQIPALAGKKITSASGAIGNKNY
                     TLGTEDGYVLPLTVTFNITFDEKQKRILTPRIIDGELVSISSEPIVFAVSKEDEEGSS
                     STVAYTKSGQLLLVTLEQTESFFGDSEITEKRANLTNQIVDEKITSLALDDLLYNVYA
                     GTVDGKILNWNIKGGKANPKFEKVIDASDSPITLLNFVLGNRSLIVGDRSGNVSTWFK
                     VKDENNIETLTKVHILASHASEVTSSASSPRDKGIITADAGGNIIVHHTTSEQKHLEI
                     KGKGDYIKSIVIAPKANGILAITKDNNLFNYTLSNEHPETNFKTLFGKVWYEGYQNPE
                     YVWQSTGGTDEFEPKFSLTPLMFGTFKGAFYALLFAIPISILGAICVSQFMHPTLRNT
                     IKPTIEIMAALPSVVLGFFAGLWMAPLMEKVIPALIAMPILITLFTLFAVYGWQFVPR
                     IIKGRFKEGYELFLLIPVIIIGVLVSLGINGPLEDLLFGGNYKEWLYTVLGLQYDQRN
                     ALVVGYAMGFAVIPIIFTISEDALSSVPQHLTAGSLALGANKWQTAIRVVLPTASAGI
                     FSAVMIGLGRAVGETMIVLMATGNTPVLDWNIFNGFRTLSANIAVEIPEAPHGGTLYR
                     ILFLAALLLFIATFLINTIAEIVRDRLRKKYGQL"
     gene            complement(5144..6103)
                     /locus_tag="AC159U_09875"
     CDS             complement(5144..6103)
                     /locus_tag="AC159U_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3337400.1"
                     /translation="MKSQFIKVLSFFIVISAFALNSTNALEVDPALSDYAKVSGVSGN
                     VSSIGSDTMNNMMALWCEGFAKYYPNVKCQIEGKGSSTAPPALIEGTSTFGPMSRAMK
                     NKEVDAFEKAFGYKPTPVKAAIDTLAVYVNKDNPISCLTIAQVDAIFSKNRACGGPHD
                     LATWGDAGLTGEWANKPISKYGRNSASGTYGYFKKKALCKGDYKDDVKEQPGSASVVQ
                     GITEDIGGIGYSGIGYKTSGVKVVEIAKDGAECAGPTIENVMAKKYPLARFLYLYVNK
                     APNTDLDPLRTEFLNYVLSKEGQNVVIKDGYIPVPANIAYGQY"
     gene            complement(6303..6995)
                     /locus_tag="AC159U_09880"
     CDS             complement(6303..6995)
                     /locus_tag="AC159U_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3337401.1"
                     /translation="MSEKTIVIVDDEQDIVELVSHHLKREGYLVKEFGKGREFFSYIE
                     SVIPDLIILDIMLPGIDGLEICKILKNKKKTASIPIIMLTAKAAESDVVVGLELGADD
                     YVVKPFSPRELVARVKSVFRRLDKKEDDHELIKIGPLSINSDKYEVLVDGKSVDLTTT
                     EFKILEVLTERKGSVFSRDQLIKKKRLWGDDKLVYDRTIDVHIKNLREKLGVAGNMIK
                     TIRGIGYKLEAM"
     gene            complement(7049..>7262)
                     /locus_tag="AC159U_09885"
     CDS             complement(7049..>7262)
                     /locus_tag="AC159U_09885"
                     /inference="COORDINATES: protein motif:HMM:NF021883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3337402.1"
                     /translation="LVDKGITREKAYALVQRNAMKCWEQKTPYKDLLISDPDVMNHLT
                     EKEIDSCFDLKGEFRNIDMIFKRVFS"
BASE COUNT         2102 a         1508 c         1201 g         2451 t
ORIGIN      
        1 actaaatccc ttggaataat tgcctgatat atctactcct atttctttta atacctttat
       61 ggaatatgga ttaatttgtg tagggtccga gccagtgcta taggcatgaa gcctgtccga
      121 atgaaaactg tttagaattc cttcagccat ttgagacctt gcagagttgt gtgtacatac
      181 aaaaagcact ttttttctat ccatattata tatatttata ttttgagatt ttatccaaat
      241 ttgccggtta cataatcctg ggtctgaggt atggttggat ttataaatat gttttgtgtt
      301 tcatcatatt caatcatctt tccaagatac ataaaagccg taagatcaga taccctggaa
      361 gcctgctgca tattatgagt cactatgaca atagtgtagt tttctttaag ctctgttatg
      421 agatcttcga ttcttgtagt agcaacagga tcaagtgcgg agcagggctc gtccataaga
      481 agcacctcag gctccactgc tattgccctt gcaatacaga gcctctgctg ctgtccgcca
      541 gacattccaa gggcgctttc atccaatctg tcgtgaacct cttcccaaag ggcagcacct
      601 tttaggcttt tttccacaat atgatcaagt tcgtcttttt cgtttatacc ggcaatcctg
      661 gctccgtagg caacattctc gtatatagtt ttaggaaaag gatttgattt ctgaaaaacc
      721 attccaactt tttttctaag ttcagtaagc tctaagtttg gatcgtttat atccctacca
      781 tttatttcaa ttgcacctga gatagtaaca ttatcaatta gatcatttaa tctgttaaaa
      841 catctaagaa gggttgattt accgcaaccc gaaggaccaa taaatgcagt tactttcttt
      901 ttgggaataa gaagattaat atcatataga gcttgtttat caccataact taagcacagg
      961 tttttaacta caactaatgg atcttctata tcctgtttaa taaatgtttc aagctgttca
     1021 ctatctgccg gtcctgtaga aatttgctta ttatcagtgc tttgttctaa ttgattttct
     1081 tccattttat ttcccgtata tccggtatcc tctaacgctg tactcattgt tattcttctc
     1141 cataaaatat tttactatat ttagtgaatt taattaaata aatattacta aatttgagat
     1201 gaactatatt ttttcttgag tctgtttcta attacaattg ctgcaagatt taacaaaatg
     1261 actatcacta tcaaaagaaa agtagttgta taaaccatag gttttgcagc ctcaacattt
     1321 ggtgactgaa atcccacatc ataaatatga aaccccaggt gcataaattt tctttctaaa
     1381 tgcacaaatg ggaaataccc atcaatgggt aaatcaggag caagcttaac aacaccggta
     1441 atcataagtg gtgctacctc tccggtagcc ctggctattg ccagaataag cccggtcatg
     1501 atggcaggcg cagcacttgg aagaaccacc ttccacatcg tctcaaactt tgtggcacca
     1561 agcgcaaggg agccttccct tatatttctt ggaacagccg caagcccttc ctcggttgca
     1621 acaattacta caggtactgt aagcagagca agagtaagtg acgcccataa tattcccccc
     1681 gtaccataag tgggcgcagg aagctgatca ctataaagta tctgatcaat tgccccacca
     1741 attccataaa tgaagaatcc aacagcaaac actccaaata ctatagacgg aacaccggct
     1801 aggttgttca cacatatcct tactatacgt accattgtac cctgtctagc atattctctt
     1861 agataaagag ccgccaatac accaaaagga agaaccacga tgctcattat tatcaccatc
     1921 atcacagtgc cgaatattgc aggaaacact ccgccttctg tgttcgcttc tcttggatca
     1981 gcagatagaa actcccagaa tttatccatg tatagcatca acttactgaa tgtacccatg
     2041 gaattgggtc tgtatgcccg gactattaat cctgcagaaa actcttttgt tctgccatca
     2101 actgtttcta atttaacttt gtattggtca agctcggtat ataaagcagt aagcttcttc
     2161 tcttctatct taaatttttc ttcctgctca gcaaactgtt tatttagttt atcaagtgtt
     2221 ttaatatcct caggatcaga ctgatttaaa ttttttttct taagccttat tgcctccatt
     2281 tcatagttaa tatcgcctat atcatgtttt tctatctctt ttatctcttt atattttgct
     2341 gtagtcctgg gaagtatttc agcaagctct gtaattgatt gctctgtagt actgctaaca
     2401 atattgcttc cttcatcgat cccggtaaca aaaccataca tatttcccca ttcgagtcgt
     2461 tcaattaaaa acgcattttc cggataagag atatttttaa tttgatcatc atcaatccac
     2521 ttaaaatcaa atccataaat atctctgttg cctactttta gctgtatcct ggtttttttg
     2581 gatttttctt tggaattagg aaccctaatt ttctctctac cgcttatttg tccaaggtat
     2641 ttcttgccgt ccacaagctc gacctgagcg atatcagaag gccagaaaaa tccgagccct
     2701 ttggatgcaa tgataaataa aagaccaaat atcgctatca gactgaacat cagcgcagta
     2761 cctgtaagcc atacaaatgg atcgccggat ttaaagaagt ttttcattat gtttatttat
     2821 taaagctgtc cgtatttttt tctaagtctg tctcttacaa tctcagcaat cgtgtttatt
     2881 aaaaaagtag caataaaaag tagcaatgcc gcaagaaata aaattctgta aagagtgcct
     2941 ccatggggag cctccgggat ctccacagca atgttggctg agagtgtcct gaatccattg
     3001 aatatattcc agtcaagtac tggagtattc cctgtggcca taagcactat catcgtttct
     3061 ccaacagccc ttccaagccc aatcatcacg gcagagaata ttcctgcact tgccgttgga
     3121 agaaccaccc tgatagctgt ctgccattta ttggcaccaa gtgcaagcga gcctgcagta
     3181 agatgctgag gaacacttga aagagcgtct tctgaaattg tgaatattat tggtataact
     3241 gcaaaaccca ttgcatagcc gactacaagc gcatttctct gatcatattg gagcccaagt
     3301 accgtgtata gccattcttt ataatttcca ccgaatagaa gatcttccag tggcccgttt
     3361 attccaagtg atacgagtac ccctattatg atcacgggta ttagaagaaa aagttcgtag
     3421 ccttccttaa atcttccctt aataatcctt ggaacgaact gccatccgta aacggcaaaa
     3481 agcgtaaaca atgtaatgag tataggcatt gcaattaaag cgggtattac cttttccata
     3541 agcggcgcca tccaaagccc tgcaaaaaat ccaagcacca cgcttggaag tgcagccatg
     3601 atttctatgg ttggctttat agtatttctt agagtgggat gcataaattg tgatacacat
     3661 atcgcaccca gaatagaaat cggaatcgca aatagaagag cataaaatgc gcctttaaac
     3721 gttccaaaca taagtggagt aaggctgaat tttggctcaa attcatctgt accacctgta
     3781 gactgccata cgtattcagg gttttggtat ccttcatacc acacttttcc aaatagagtt
     3841 ttaaaattgg tttcaggatg ttcgttgctt aaagtgtaat taaacagatt gttatctttt
     3901 gtaatagcaa gtattccgtt tgcctttggc gcaataacaa tactttttat atagtcgcct
     3961 ttacctttaa tctccagatg cttttgttca gaagtagtat gatgaacaat aatgttccct
     4021 ccggcatctg ctgttattat tcctttatct ctaggagaag atgcagaact ggtaacctct
     4081 gaggcatgtg atgctaagat gtgaactttt gtaagagttt caatattatt ttcatccttt
     4141 accttaaacc aggtggacac atttcctgac ctatctccaa caattaacga cctgtttcca
     4201 agaacaaaat tcagaagtgt aatggggctg tctgaggcat ctatgacttt ttcaaatttt
     4261 gggtttgctt taccgccctt aatattccag ttaagaattt tgccgtctac agttccggca
     4321 taaacgttat aaagtaaatc atcaagtgcc aggcttgtta ttttttcatc tacaatttga
     4381 ttagttaaat ttgctctttt ttctgttatt tccgagtcac caaaaaatga ctccgtctgc
     4441 tccaatgtca caagtagtag ttgtccagat tttgtatatg cgactgtgga gcttgaacct
     4501 tcttcatcct ctttactcac agcaaaaact atgggttcag aagatattga tactaattca
     4561 ccatcaataa tacggggagt taaaattctt ttctgttttt catcaaatgt aatattaaat
     4621 gtaacggtaa gtggtagtac ataaccatcc tcagtgccta atgtgtaatt cttgttacca
     4681 atcgctccgg atgcggatgt aatctttttt ccggccagag caggtatctg tatttttttt
     4741 aaatttttac cgtttgctaa atcaataaaa tgaacatatc cgttatctgt aatcagatat
     4801 gcaatttctc taaactcttc tacaccaatt gctacaatat cgggttttgg tgtaagagct
     4861 aatgatgagt tttcggatgg tggtactgcg ggtagtataa atatagattt gttttcattt
     4921 agatcaaaat tactagctac ggttgtctta actttttccc aaagagggac aacttcgatc
     4981 ccaatgaaaa gaaggatagc aataatactc agtattatag ctatccctcc tgatgtaact
     5041 acaacggtag caattttatc tgcaagccgc ctttttctaa ttgctttttc tttagaggcg
     5101 gcctgcgaat ttattttttt ttctgattca gtaataactt ccattaatac tggccataag
     5161 caatatttgc tggtaccgga atataaccgt cttttattac aacattctga ccttctttac
     5221 taagaacata gtttaagaac tcagttctaa gtggatcaag atctgtatta ggagctttgt
     5281 tcacatatag atagaggaat cttgccagag gatatttttt agccattaca ttttctattg
     5341 taggacctgc acattctgct ccgtcttttg caatttcaac aacctttact ccggatgttt
     5401 tataacctat gccgctataa cctattccac ctatatcttc agtaatgccc tgcaccactg
     5461 aagctgagcc tggctgttct tttacgtcgt ctttatagtc acctttacag agtgcttttt
     5521 tcttaaagta accataagtt ccagatgcag agtttctgcc gtatttactt attggcttgt
     5581 ttgcccattc tcctgtaagt cctgcatcgc cccatgtagc taaatcatgc gggccgccac
     5641 aggctctgtt cttggaaaat atagcatcaa cctgagcaat tgttaagcag cttattggat
     5701 tatccttgtt tacataaact gcaagagtat caattgccgc cttaacaggg gttggcttat
     5761 aaccaaaagc cttttcaaat gcatctactt ctttattttt catggctctg gacatcggtc
     5821 caaatgttga agttccctca ataagtgccg gtggcgctgt gcttgagccc ttaccttcaa
     5881 tctgacattt tacatttgga taatacttag caaatccttc acaccaaagc gccatcatat
     5941 tgttcatggt atcagagcca atgctgctaa cgtttcctga aacaccgctt acttttgcat
     6001 aatcactaag tgccggatct acttctaaag catttgtact gtttagagca aatgctgata
     6061 taacaataaa gaaacttaaa acctttatga attgagattt tattctcact gtactacctc
     6121 ctaataattt tcgttctttg attaattaaa ctcatcaatt gttaagattg tgttaagaaa
     6181 gtgagaaata tgtattaata ttgtacaaat attttagtac agatatgaaa tgttgttgaa
     6241 ttacagcagg ttacgtcagg agatctatga aagttaatta ataaaaagta gttaataggt
     6301 ttttacattg cttctaattt ataacctatc cctctaatgg tttttatcat attgccggca
     6361 acaccgagct tttctcttaa gtttttaata tgaacatcga ttgtacggtc ataaacaagc
     6421 ttatcatctc cccaaagcct ttttttcttt ataagctgat ctcttgagaa tacactgcct
     6481 tttctctcgg taagaacttc taaaatcttg aattcggttg tagttagatc aacgcttttt
     6541 ccatctacta atacttcata tttatcagaa tttattgaaa gtgggccgat cttgattaat
     6601 tcatgatcat cttctttctt atccagtctc ctaaaaacag atttcaccct ggcaactagt
     6661 tctcttggac taaaaggctt tacaacatag tcatctgctc caagctccag tcctacaacc
     6721 acatcagatt cagctgcttt tgcagttaac ataataatag ggatagaagc agtctttttc
     6781 ttatttttta gaattttaca tatttccagg ccgtctatgc ccggaagcat aatatccaaa
     6841 ataatgagat ccgggataac cgattctata taagagaaaa attctcttcc cttgccaaat
     6901 tcctttacca gatagccttc tcttttgaga tgatggctta caagctcaac tatatcctgt
     6961 tcatcgtcta caattacaat cgttttttca gacatattgt atttttctaa atatgttcca
     7021 atacaaccat tataagacag aaaatttttt aagaaaatac tcgtttaaat atcatatcta
     7081 tattcctgaa ttcacctttg agatcaaaac agctatcaat ttctttttct gtcagatgat
     7141 tcattacatc aggatctgaa attaagaggt ctttataggg ggttttttgc tcccagcatt
     7201 tcattgcatt tctttgtacc agtgcataag ccttttctct agtaattcct ttatcaacaa
     7261 gc
//
LOCUS       DBNGSV010000266         8006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_671780, whole genome shotgun sequence.
ACCESSION   DBNGSV010000266 DBNGSV010000000
VERSION     DBNGSV010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_671780"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC159U_09890"
     CDS             <1..416
                     /locus_tag="AC159U_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3337403.1"
                     /translation="LKDLTGDKMLPIWIGFMEASSIAMELEKTPRLRPLTHDLVKNIL
                     DDLQYSVSKVELTDLKDNTYYATIHLKKSDDIHILDSRPSDAIAIAIRTSSQIFVHEN
                     VIESSKQLQAVDEDKEKLAEILDKIPPGDFGKYKM"
     gene            complement(434..1852)
                     /locus_tag="AC159U_09895"
     CDS             complement(434..1852)
                     /locus_tag="AC159U_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3337404.1"
                     /translation="MGAANNGRIIVVDDEPGMREFLEIMLQKDGYSVVTAGDGKHAED
                     KINSQIFDLAIIDVQMPTMDGIDLLKKLNEKAPDTTVIMITAFASHESAIEAMKHGAY
                     DYITKPFKIDEIKLVIKKALEKKRLVSENTRLKRELETKFGFGNIIGRSPAILQVFEL
                     IKRVAELNVNVLITGESGSGKELVARAIHYAGNRSEGSFVPVNCGAIPETLIESELFG
                     YKKGSFTGAVRDKKGLFEEAHKGTIFLDEIADLPIHLQVKILRAIEEKMIRSLGTVDA
                     TPIDLRVIAATNKSLEDEIAEGRFREDLFYRLNVIKINLPPLRERREDISPLAMSFVN
                     KYSEEMGKEIRGISPKALEILENYSYPGNVRELENIIARSIALESTNIIRHETLPPLV
                     SGKDYVEIGGDDLSSDTNLDAMLDNIEKKMLLKALESTGGNKTEAAKKLGITLRSIRY
                     RLVKHGIDGEGDSDGLTAEAAK"
     gene            complement(1876..3504)
                     /locus_tag="AC159U_09900"
     CDS             complement(1876..3504)
                     /locus_tag="AC159U_09900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3337405.1"
                     /translation="MFFNWLDSTINRIVLIRILVATLLFGSSALIYFLNGRTRVALYL
                     LFVVAVIYIFSLVYLSFKDVISRYKTLIDSFQIFLDVLLSSIIIVVTGGLGSPFIFLF
                     ALIIIYTNIIFSNRLVSYALSFFLAIILLVIVASKPRIFHSDDSYQLRLQASMQIDQQ
                     SLVSTYFNLAGFLLAAILSGYLSEKTRIVRKELGQSKKSLSILQNINDNILKSLDSGV
                     ITLDLKGNIISANPMSKNILDLQSDEQIINKDIYEFFPALDLDNLISTKREQILYTTS
                     EGEKITLGFSSSLLQGDDDQTQGYILIFQDLTEIKILEDKLRNTEKLAILGQLASGLA
                     HEIRNPLSAISGAIEILSSEVESSSDNLRLVNVATQEIERMNLIVEDFSILTNPILKT
                     GLPVDMSVILNETLDTFSKTIKRSDINLERQIQSEIFVKGDSYRLKQSVWNLLLNSMQ
                     SISGQGTIRIEALVEKENVIVKISDTGCGISQKNISSIFDPFFTTKNSGTGLGLVIVQ
                     KVIEGYNGKIDVVSAVNEGSTFIITMGKETLNDE"
     gene            complement(3552..4766)
                     /locus_tag="AC159U_09905"
     CDS             complement(3552..4766)
                     /locus_tag="AC159U_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3337406.1"
                     /translation="MAVYTWQGRLRGRIEKGEMEAPSRSAVLARLRGMQIQPIPDKIK
                     EKSSILNMNIAFGGVPTRDLVVFTRQLSTMIDAGLPLVRSLEVIREQQPNAAFKEIIG
                     RVKEDVEGGATFADALEKHPKAFDTLYSNLARAGESGGVLDVVLQRLATYLEKIEGIK
                     RRVKGAMIYPAVVISVAIIVLAVIMIFVVPTFAELFKDLGTTLPSLTQTIVNISDWLK
                     SNIIYILIAIVLMVIGVSALYRRSLKARRLFDGFLLKLWLIGTLLLKTVIARFCRTLA
                     TLTAGGLPILDGLEITAKASGNVVVEEAIMDARKSVSEGQLLAEPLATRPKIFPPMVV
                     QMISVGEQTGNLDDMLNKIADFYEDEVDVAVAALMSALEPAMIFFLGCTVGIIVVAMY
                     LPMFKLVATMAG"
     gene            complement(4786..5886)
                     /locus_tag="AC159U_09910"
     CDS             complement(4786..5886)
                     /locus_tag="AC159U_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3337407.1"
                     /translation="MGIQLSQLLKVMVDNGASDLHITTNSPPVIRVDGKLVPIKHPPL
                     TPPETKDLCYSVLTDMQKHKFEENWELDFSFGMQQLGRFRGNIYMQRGVVAGAFRLIP
                     YKIRDLNELGHPPVVHDLIKKPRGLILVTGPTGSGKTTTLAAMIDQINQERREHIVTI
                     EDPIEYMFSHKNCLVNQREVGSDTKSFHNALRSVLREDPDVILIGEMRDLETIKIALT
                     LSETGHLTFATLHTNTAVQTINRVIDVFPPHEQPQVRAQLSFVLEGILCQTLIPRVGG
                     GRVLGVEILVPNPAIRNLIREDKVHQIYSQQQIGQEKWNMQTLNQSLVDLYLRKQITY
                     EDTLTKSQEPDEIKRILDEQNSAQGNKRAIRR"
     gene            complement(5903..7657)
                     /gene="pilB"
                     /locus_tag="AC159U_09915"
     CDS             complement(5903..7657)
                     /gene="pilB"
                     /locus_tag="AC159U_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HIR3337408.1"
                     /translation="MKQQKAGAKMGDILIKEDVINLDQLKSAIQEQKHSGRKLGETLL
                     NLGYIDENQLVAYLSKQYAVPAINLEQFDVSEEALDLVPRDSALKHKLVPIDRSGSTL
                     VVAMADPSNIFAIDDLKFATGQNIEVVVASERSIKTAIEKHYGSQEEWQKKVEAEKSQ
                     EEVGKLFGDLEDFLLDIATVEDLDLGDLKEASEEAPVIRLVNHILLESIRRGASDIHI
                     EPYETDYRVRLRIDGVLYDILKPPPQLKNPVSSRIKVMANLDIAERRLPQDGRIKIKA
                     GKGRTMEYRVSCLPTITGEKVVMRLLDQDSLKLDLTQLGFNEDELKLFRESIYQPFGM
                     VLITGPTGSGKTTTLYSGLMELNREDVNISTAEDPVEYSLSGVNQVQMHEAIGLNFSS
                     ALRSFLRQDPDIILVGEIRDYETAEISIKAALTGHLVLSTLHTNDAPSTITRLLNMGV
                     EPFLLTASLNAILAQRLVRKVCPDCQEVVDVSPQVLIDLGVSPKAVSDYVIYRGSGEN
                     CKTCMGTGYKGRIAIYEVLRITEEIKEFILSGASALELKREAIRQGMHTLRQSALLKL
                     KQGITTIEEVVRNTSQDD"
BASE COUNT         2292 a         1730 c         1298 g         2686 t
ORIGIN      
        1 tacttaaaga cttaacaggg gataagatgc ttcctatctg gatagggttt atggaagcaa
       61 gttccatagc gatggagctt gaaaaaacac cgagactaag accactcacc cacgatcttg
      121 ttaaaaatat tttagacgac ctgcagtatt ctgttagtaa agttgagcta actgacttaa
      181 aagacaatac atattatgca actattcact taaaaaaatc agatgatatt catatactgg
      241 attccagacc aagcgacgca atagcaatag ccattagaac aagctcccaa atctttgtac
      301 atgaaaacgt tatagaaagc tcaaagcaat tacaggcagt tgatgaggat aaagagaaac
      361 ttgcagagat cctggataaa ataccccctg gagattttgg gaaatacaag atgtaacaac
      421 aataagaatt tagctactta gctgcctcag ccgtaagacc atcactatct ccttcaccat
      481 caattccatg tttaacaagt ctgtatctta ttgaccgaag agtaatacct agttttttag
      541 cggcttcggt tttatttcca cctgtagact ccaatgcttt aagcagcatc ttcttttcta
      601 tattatcaag cattgcgtcc aggttagtat cagacgacaa gtcatccccc cctatctcca
      661 catagtcttt accgcttaca agaggcggta gggtttcgtg tcttattata ttagttgact
      721 caagtgcaat agagcgtgca ataatatttt ctaactctct tacatttccc ggataactat
      781 aattttccag gatctcaagc gcttttggtg agatacctct tatctctttt cccatttcct
      841 cagaatattt attcacaaaa ctcatcgcaa gtggtgaaat atcttctctt ctctctctaa
      901 gtgggggaag attgatctta atgacgttta acctgtaaaa cagatcttct ctgaacctgc
      961 cttcggcaat ttcatcctct aagcttttat ttgtagcagc aatcacccta agatcaatcg
     1021 gtgttgcgtc aacagttcca agggatctta tcattttctc ttctattgcc cttagaatct
     1081 ttacctgtaa atgtattggc agatcggcaa tttcgtctaa aaatattgta cctttatgag
     1141 cctcttcaaa taatcctttt ttatccctta ctgcacctgt aaaagagcct ttcttgtatc
     1201 caaacaactc actctcaatc aatgtttcag gaattgctcc acagttaact ggtacaaacg
     1261 atccctcact tctgtttcct gcgtaatgta tagctcttgc tacaagctcc ttacccgaac
     1321 cgctttctcc tgtaatcaaa acatttacat tcagttcggc aaccctctta atgagttcaa
     1381 aaacctgcag gattgcaggg cttcttccaa ttatatttcc aaatccaaac ttcgtttcca
     1441 gttcacgttt gagtcttgta ttttcactaa caagcctttt cttttccaga gcttttttta
     1501 ttacaagctt tatttcatct attttaaatg gttttgtaat ataatcgtat gccccgtgtt
     1561 tcatggcctc tattgctgat tcgtgcgatg caaaagctgt aatcataata actgtggtat
     1621 caggggcttt ttcatttagt tttttaagca gatcgatgcc gtccattgtc ggcatctgca
     1681 catcgattat agcaagatca aaaatctgag aatttatttt atcctcggca tgttttccat
     1741 cgccggctgt tacaacacta tatccatctt tttgcagcat tatttccagg aactccctca
     1801 tccccggttc atcatctact acaataattc ttccgttgtt agctgccccc attgcaaatc
     1861 tcctcaaaat gcttgttatt catcatttaa tgtttctttt cccattgtaa ttataaatgt
     1921 agatccctca tttacagcac ttacgacatc aatcttgcca ttataaccct ctattacctt
     1981 ttgcacaatc acaaggccca gtcctgtacc cgaatttttg gtggtaaaaa aaggatcaaa
     2041 tatacttgaa atatttttct gactaatacc gcaaccagta tcagaaattt ttactatcac
     2101 attttctttt tccaccaagg cttctattct aattgtcccc tgacccgata tagactgcat
     2161 tgaatttaga agcaggttcc agacagattg ttttagtctg tagctgtcac cctttacaaa
     2221 tatttcactt tgtatctgcc tttctaaatt aatatccgat ctctttatag ttttagagaa
     2281 cgtatctaac gtttcattca agattacaga catatcaacc ggcagacctg tttttaaaat
     2341 tggattcgtt aatatcgaga aatcttctac gatcagattc attctctcga tctcctgtgt
     2401 tgctacgttc acaagtctta gattatctga gctgctctcc acctcactgc taagaatttc
     2461 tatcgctccg ctaatagcag atagtgggtt tcggatttca tgagccaggc ccgaggcaag
     2521 ctgtccaagt attgccagtt tttctgtgtt tcttagttta tcctccagga tttttatttc
     2581 tgtaaggtcc tgaaatatca gaatataccc ctgtgtttga tcatcgtctc cctgcagtaa
     2641 agatgaagaa aatcccagag taatcttttc cccctcactt gtggtatata atatttgttc
     2701 ccgttttgtc gaaataagat tatctaaatc taatgctggg aaaaattcat aaatatcctt
     2761 gtttattatc tgctcatcag actgcaggtc caatatgttt ttgctcatag ggttggcaga
     2821 tattatgttt ccttttagat cgagtgttat gactccgcta tcgaggcttt taagaatatt
     2881 atcgttaata ttttgtaata tactaagact cttcttactc tgaccaagtt cttttcttac
     2941 aatcctggtt ttctctgata aatagccact aagaattgca gcaagtaaaa aacctgcaag
     3001 gttgaagtat gtacttacca gactttgctg atctatttgc atacttgcct gtaatctcaa
     3061 ttgatatgaa tcatcggagt gaaatatcct tggttttgat gcaactatta caagtaatat
     3121 tattgcaaga aaaaatgata aagcgtaact taccaatctg ttggaaaaaa taatatttgt
     3181 ataaattatt ataagagcaa ataggaatat gaatggacta cccagtcccc ctgttactac
     3241 tataataatg gatgaaagta atacatctaa aaaaatctga aaggagtcga taagagtttt
     3301 gtatctagat attacatcct taaatgacag atatacaagg ctgaatatat atattacagc
     3361 tactacaaac agcagatata gcgcaactct agttctaccg tttaggaaat atataagggc
     3421 agaagaaccg aaaagtaggg tcgcaactaa tattcggatc agtactattc tattgatagt
     3481 cgaatctaac caattaaaaa acattcatta agttgtttag caagtaaggt aattagaata
     3541 gtattatatg tctatccagc catagttgca actagtttaa acattggcag atacattgct
     3601 actactatta tacccactgt acaacctaaa aagaatatca ttgccggttc gagagcggac
     3661 ataagtgctg caacagcaac atctacctca tcttcataga agtccgcaat tttattaagc
     3721 atgtcgtcca gattaccagt ttgctcacct acagagatca tctgaacaac cataggaggg
     3781 aatatcttag gtcttgtagc aaggggttca gcaagaagct gtccttcgct tacactcttt
     3841 cttgcatcca ttattgcctc ttcaaccact acatttccag atgcttttgc ggtaatttcc
     3901 agtccatcaa gaataggtaa accaccggcg gttagtgtag ccaatgtacg gcagaacctt
     3961 gctattacag tttttaataa taatgttcca ataagccaga gttttagtaa aaatccatca
     4021 aataacctcc tggcttttaa ggagcgtcga tatagagccg aaacacctat aaccatcaga
     4081 acaattgcaa ttaatatata tattatatta cttttaagcc agtcacttat gtttacaata
     4141 gtctgagtta gagacggaag cgttgtgccc agatccttaa aaagttcagc aaatgtagga
     4201 accacgaata tcatgattac agcaagcaca ataattgcta cagaaataac aacagcagga
     4261 taaatcatcg cacccttcac ccttctctta ataccttcta ttttttccag gtatgtagca
     4321 agtctttgta aaacaacgtc taaaacacca cctgattctc cggctctggc tagatttgag
     4381 tataaggtat cgaaagcctt aggatgtttt tccaaagcat cggcaaaggt cgcacctcct
     4441 tcaacatctt cctttactct tccaattatc tctttaaatg cagcatttgg ctgctgttct
     4501 ctaattacct caagtgatct tacaagagga agacccgcat ctatcatcgt agacaactgc
     4561 cttgtaaaaa caaccaaatc tcgggttgga acaccgccaa aagcaatatt catatttaat
     4621 atactgcttt tctcttttat cttatcagga ataggctgaa tctgcatacc ccttaatcgt
     4681 gccagtactg cagatctgct tggggcttcc atttcacctt tttctattct tcctctaagt
     4741 cttccctgcc aagtataaac agccatatta tttactcctt aatgtttatc ttctgattgc
     4801 tctcttgttt ccctgtgctg aattttgctc atccagaatt cttttaatct catctggttc
     4861 ctgactcttc gttaacgtat cttcgtatgt aatttgtttt ctcaaatata aatcaaccag
     4921 ggattggttg agcgtctgca tattccattt ttcctgtcca atctgctgct gtgaataaat
     4981 ctgatgcact ttatcttctc ttattagatt tctgattgcc gggttcggaa ccagaatctc
     5041 aacccctaat acccttcctc ctccaacacg tggtattaat gtttggcata agataccctc
     5101 taagacaaaa gacagctgtg ctcttacctg gggttgttcg tggggtggaa atacatcaat
     5161 tacacggtta attgtttgaa cagccgtatt tgtatggagc gtggcaaatg ttaaatgtcc
     5221 ggtttcagac agagtaagag caatcttgat agtctcaagg tctctcatct caccaatcag
     5281 gattacatca gggtcttctc taagtacact tctaagtgcg ttgtgaaagc tttttgtatc
     5341 acttcccact tctctttggt ttaccagaca gttcttatga ctaaacatgt attctatagg
     5401 atcttcgatc gtaacaatat gctctcttcg ttcctgatta atctggtcta tcattgcagc
     5461 aagagtcgta gtttttcctg accccgtagg tccggtcact agaattaaac cccttggttt
     5521 tttaataaga tcatgaacaa caggcggatg ccccagttca tttaaatctc taattttgta
     5581 cggaataagc ctaaaagcac ctgctactac acccctttgc atatagatat tgcctctaaa
     5641 ccttccaagc tgctgcatac caaaagaaaa gtcaagttcc cagttttctt caaacttatg
     5701 cttttgcata tctgttaata cgctatagca aaggtcttta gtttctggtg gagtaagggg
     5761 gggatgctta ataggaacaa gcttaccatc aactcttatt acaggaggtg agttggtagt
     5821 aatgtgtaaa tctgaagctc cgttatcaac cattaccttt aacagttgac taagttgaat
     5881 gcccataaat tatcctccca aatcaatcat cctgagatgt atttctaact acctcttcaa
     5941 ttgttgttat accttgcttg agttttagaa gtgcactttg tctaagagta tgcattccct
     6001 gtcttatagc ttctctttta agttcgagag ccgacgcacc gcttaatata aattctttga
     6061 tttcttcagt aattcttaat acttcataga ttgcgattct tcccttatat cctgtaccca
     6121 tacaggtctt gcaattttct ccgcttcccc tgtaaatcac ataatctgac actgctttag
     6181 gtgaaacccc aagatctatg agtacctgtg gagatacatc cacaacctct tgacaatccg
     6241 ggcatacttt tcttacaagt ctttgagcaa gtattgcatt gagtgaagct gtaagcaaga
     6301 atggctcaac acccatgttg agaagtcttg taatagttga aggggcatca tttgtatgta
     6361 aggtagatag aacaaggtga ccggtaagag cagctttaat ggatatttct gcagtttcgt
     6421 agtctcttat ttcacctact aatataatat ctggatcctg cctgaggaac gatcttaatg
     6481 cagacgagaa attaagcccg atcgcctcat gcatctgcac ctgatttact ccggacaaac
     6541 tgtattctac cgggtcttct gctgtggaaa tgtttacatc ttctctgttt agctccatca
     6601 gtccagaata cagtgttgtt gttttaccac tacctgtagg acccgtaatt aaaaccatac
     6661 cgaaaggctg ataaattgat tctctaaata gtttaagttc atcttcatta aaaccgagtt
     6721 gtgtcaggtc aagcttcagg gaatcctgat caagaagcct cataaccact ttttctccgg
     6781 ttattgttgg aagacaggaa acacgatatt ccatggttct gcccttaccc gcctttatct
     6841 ttatccttcc atcctgtgga agccttcttt cagcaatatc gagatttgcc ataactttaa
     6901 ttcttgatga aacaggattt ttaagctgtg gcggaggttt aagaatatca tacagtactc
     6961 cgtcaattct gagtcttacc ctataatcag tctcataagg ttctatatga atatcactgg
     7021 ctcctcttct tattgattct aatagtatgt ggtttaccag tcttattacc ggtgcttcct
     7081 cagaagcctc tttgagatct ccgaggtcaa gatcttctac tgtagcaata tctagtagaa
     7141 agtcttctaa atcgccaaaa agttttccga cttcttcctg actcttttct gcttcaactt
     7201 tcttttgcca ttcttcctga gaaccatagt gcttctcaat tgctgtctta atcgatcttt
     7261 ccgaggctac aactacttca atattctgac ccgtagcaaa ttttaaatca tcaatggcaa
     7321 aaatgtttga gggatctgcc atggcaacaa ccagagtaga tcctgatctg tctatgggaa
     7381 ccagtttgtg tttgagcgct gaatcccttg gtaccaggtc cagggcttct tctgatacat
     7441 cgaactgctc aaggttgatt gcaggtactg catattgttt actcaaatat gcaacaagct
     7501 ggttttcatc tatgtatcca aggtttagca gagtctcacc aagcttccta cccgagtgct
     7561 tttgctcctg gattgcgctt tttaactgat caagatttat aacatcttct ttgatcagaa
     7621 tatcgcccat cttggcgcct gctttttgct gcttcatcta taaattatac ctcgaaatta
     7681 aaaaactatg taataaatat taatagtgca atatatgtgc caataccatc ggctaatttt
     7741 agctgattag ataaatcgaa gtatttatcc ctccctataa gataatttct tcatactttt
     7801 aaccctctta ccctctatcc tttatactac atttttccta taaagtcaat ctgcatgtcc
     7861 tatataattt gttctcataa tgtaggcata tttaatctca aaacttaaag taaactctaa
     7921 aataatacat attacttttt atatcattta ttttatatgc actatttcat ttttcttttg
     7981 atacggtaaa taatatctag tatata
//
LOCUS       DBNGSV010000267        14726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_463899, whole genome shotgun sequence.
ACCESSION   DBNGSV010000267 DBNGSV010000000
VERSION     DBNGSV010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_463899"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /gene="thiC"
                     /locus_tag="AC159U_09920"
     CDS             <1..1695
                     /gene="thiC"
                     /locus_tag="AC159U_09920"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3337409.1"
                     /translation="YDTSGPYTDPEVDIDVLKGLDPIRAGWIQERGDVEQLDDFSSEF
                     SRNSAKNGNGITFKNLRKPLRAKKGSNVTQMHYARKGIVTPEMEYIAIRENQQIDRLG
                     EKYKELAAYHPGEKFGAGMPDSYITPEFVRDEVAKGRAIIPNNINHPESEPMIIGRNF
                     LVKINANIGNSAVSSSIEEEVEKAVWACRWGADTVMDLSTGDNIHETREWIIRNSPVP
                     IGTVPIYQALEKVGGKAEELNWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAGRV
                     TGIVSRGGSIMAKWCLSHHKESFLYTNFPKICKIMKDYDIAFSLGDGLRPGCIADAND
                     AAQFAELKTIGELTKTAWENDVQVMIEGPGHVPMHMIKENMDKQLEWCDEAPFYTLGP
                     LTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKKDVKEGVIAYKIAAH
                     AADLAKGHPTAQFRDNVLSKSRFDFQWEDQFNLSLDPDTAKEFHDETLPSPNAKVAHF
                     CSMCGPKFCSMKITQEVRDYAKEYHLTDIDALEKGMEEKSDEFSSKGGEIYLEPVD"
     gene            1851..2897
                     /locus_tag="AC159U_09925"
     CDS             1851..2897
                     /locus_tag="AC159U_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HIR3337410.1"
                     /translation="MDLQQIADEVSKSTNINVSKTDIERLVSILRKKTNPWEIIDRCD
                     FPIPAVFESIERLNSTDIVNITDKGIELTNKGRESASELHPVEDLSCKTCEGRGIDLK
                     KFAPVREKFIQLQRKRPKPSHDYDQGYVTPHSTFSRFLAAYERGDIYAKDILIIGDDD
                     LLSIVLGLSGMPKSITVLEIDKRLTKFIAQIAEQEKFEVNIEPFDMRKPLPKKYIKSF
                     DTFFTDPPETLKAADAFVGKGISTLRSAGCSGYFGFTRREASLNKWYDLQKLLTSYKV
                     VVTDIMHNFNEYINWGYEKETRAWELSPLKIKPKKNWYRSAFYRIVTLKGYKGSSKNY
                     GTQNIYEDIESSTT"
     gene            2905..6120
                     /gene="carB"
                     /locus_tag="AC159U_09930"
     CDS             2905..6120
                     /gene="carB"
                     /locus_tag="AC159U_09930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3337411.1"
                     /translation="MPKRTDIKKILLIGSGPIVIGQACEFDYSGTQACKALKEEGFEI
                     VLVNSNPATIMTDPSISDKTYIEPLIPEILEKIIEKERPQALLPTIGGQTGLNLAVSL
                     AESGVLDKYGVELIGANLEAIKKAENRDKFKDAMISIGLQVPESGVARTMEQAWEIVK
                     RVGYPVIIRPSFTLGGVGGSIAYNKEEFEDFSKAGLHMSPVTEILVEQSVIGWKEFEL
                     EVMRDSADNVVIVCTIENFDPMGVHTGDSITVAPSQTLTDKEYQRLRDASIAIIREIG
                     VDTGGSNIQFGINPENGDIVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IANDITKYTPASFEPSIDYVVVKIPRFAFEKFPQTEPLLTTQMKSVGEVMAIGRTFKE
                     SMQKAIRSLEIDSAGFESKTDDLGIIKDKLRKPNPDRLWYLADAFRAGLDLEEIFDLT
                     YIDPWFLQNIKQIVDAETDLIKSSKDGGVSVDDFRLLKQYGFSDERLGFLLGKSEDEI
                     RNTNLDNGIKCVYKMVDTCAAEFEAHTPYLYSSYEQEDEALPTDRKKVIILGGGPNRI
                     GQGIEFDYCCVHAAFSLKEEGYEAIMINCNPETVSTDYDTSDRLYFEPLTLEDTLAII
                     NREKPEGVIVHLGGQTPLKLAVPLEQNGVKIIGTSPDNIDLAEDRERFRDLILKIKLK
                     QPRSGIARSEEEALAVAGEIGYPIVVRPSYVLGGRAMEIVYTEKSLRRYIKDAVKVSH
                     NHPVLIDEFLKDAKEVDVDAISDGKTVVVGGILEHIEEAGVHSGDSAMVLPPYSLDTE
                     IVDEIKRQTKELALALNVIGLCNIQFAVKDGEVYLIEVNPRASRTIPFVSKAIGVPLA
                     NLGTKVMTGKTLKELGFTKEITPKHICVKESVFPFIRFSGVDTILGPEMKSTGEVMGI
                     DTDLRKTFAKALLAAGSELKTEGSAFISVKDDDKDKMLPVAKHLVEIGYKLIATKGTH
                     KFLKKNGIECESVKKVYEGRPNIVDFIINGDVQLVINTTFGEDEIKQSYSIRRESLMG
                     NVPYFTTISGAGAAVEAIDALKNEGLDVKPIQDYYLDSNLN"
     gene            6166..6642
                     /gene="greA"
                     /locus_tag="AC159U_09935"
     CDS             6166..6642
                     /gene="greA"
                     /locus_tag="AC159U_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR3337412.1"
                     /translation="MSIELVPITPDGYEKLKKELENLIRVERPSVIKAIEEARAFGDL
                     SENAEYDAAKEKQSFVEGRIQELESKLSRAEVIDPKSITNKERVTFGLTVKMEDLDTG
                     EEVTYQLVGPDETEPENGKISITSPIGRAMLGKQVDEDVTVTTPGGIREFEVLDIL"
     gene            complement(6693..7709)
                     /gene="ruvB"
                     /locus_tag="AC159U_09940"
     CDS             complement(6693..7709)
                     /gene="ruvB"
                     /locus_tag="AC159U_09940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3337413.1"
                     /translation="MEQRLIAPEENADDSSFDNNLRPRFLNEYLGQKNAKEKILICIQ
                     AARKRKEVLDHLLLYGPPGLGKTTLAHIVANELGVKIHATSGPVVERVGDLASILTNL
                     SEGDVLFIDEIHRLNRIVEEYLYSAMEDFKIDLMIGEGPSARSMKINVNHFTLIGATT
                     RTGLITSPLRSRFGLDLRLDYYEPDELEDIIKRSANILEVKINSEGTKEIACRGRGTP
                     RIINRLLKRVRDYADVKADGEIDIKVAKEALEMFEVDSIGLDKIDRIILLSIIEKFGG
                     GPVGIDTIAAAVSEDKDTIEDVYEPYLIRQGLLQKTPRGRTVTLNAYEHLNLPKPTKQ
                     AELF"
     gene            complement(7711..8307)
                     /gene="ruvA"
                     /locus_tag="AC159U_09945"
     CDS             complement(7711..8307)
                     /gene="ruvA"
                     /locus_tag="AC159U_09945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3337414.1"
                     /translation="MISLLKGIIAKKAPEEVIVDVGGVGYGVLVPSSTYFQLPEFGNS
                     VELHIYTHMKENTLELFGFLSGEEKEVFKILLGVSGIGPRASVNILSHISPHDLVAEI
                     SSGNLAKKKIPGIGPKLAARITTELKDKIKLLDIKTPVGAAKSGDNDLDDVISVLSNL
                     GYRPNEIDENLHTIKELINDNSNIEVVIKESLKIMKQV"
     gene            complement(8291..8836)
                     /gene="ruvC"
                     /locus_tag="AC159U_09950"
     CDS             complement(8291..8836)
                     /gene="ruvC"
                     /locus_tag="AC159U_09950"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3337415.1"
                     /translation="MIVIGIDPGTIVSGYGIVEVRDSGFNCIDSGFISQPQSRSIPSR
                     LKHIYGKLSELIEQFSPDEMSIEDVFFSKNPRSTLKLGEARGVSILAAATADIPVFEY
                     SPTEVKSAVTGNGRATKLDVQKMISTMLNIDYFEKSDTSDALAIAICHLNSIEIKKKY
                     GFTIRKKSRSKKRFTLNDIPS"
     gene            complement(8843..9592)
                     /locus_tag="AC159U_09955"
     CDS             complement(8843..9592)
                     /locus_tag="AC159U_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3337416.1"
                     /translation="MSGHSKWANIKHKKASVDAKRGKVFTKLIRELTVAARAGGGDPE
                     ANPRLRSAINTAKGQNMPNDTIDRAIKRGTGEAGVDELHELSYEGYGPGGTAVLVNAV
                     TDNKNRTVSEVRKIFNKHGGNMGEAGCVAWMFEKKGRIAFNKSSINEDELMELALDAG
                     ADDVIDDDFEFAVLTEPGVFEEVRSALEQAGLKQESAEVTMIPQNVVKVEGKEAGQLL
                     RLMEALEDSDDVQDVYSNFDIPDEMLEAVGQ"
     gene            10004..11209
                     /gene="rho"
                     /locus_tag="AC159U_09960"
     CDS             10004..11209
                     /gene="rho"
                     /locus_tag="AC159U_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3337417.1"
                     /translation="MTIARELDIEEAPRMSKQDLIYAVLRAQSAKEGSIIAEGVLEIL
                     SEGYGFLRSPKYSYLPGPDDIYVSKSQIRSFNLKTGDTIGGQVRLPREGEKNLALLKI
                     ETVNHESPEVCRERVAFENRVPLHPEEKLNLEFDKSEFCTRVMELFVPVGKGQRGLIV
                     APPRTGKTILMQHIANAITKNNPEVVLIVLLIDERPEEVTDMERSVNGEVISSTFDEP
                     SQRHIQVSDMALEKAKRLVEHGKDVVILLDSLTRLARASNTVTPASGRVLSGGMEANA
                     LQRPKRFFGAARNTEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEVYLDRKLAD
                     KRIFPAIDLQRSGTRKEELLLKEDVLTRVWLLRKVLSPMNTVEAMEFLLDKIRGTKSN
                     KEFLNMMNQ"
     gene            11277..12290
                     /locus_tag="AC159U_09965"
     CDS             11277..12290
                     /locus_tag="AC159U_09965"
                     /inference="COORDINATES: protein motif:HMM:NF027495.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HIR3337418.1"
                     /translation="MKKVIKGWNHIPGIHCGSAALRDVATFYGLPLSEPMCFGLGGGL
                     GFFYSIDNEISPTRNIHLRGPDMEPGFFSLFTTEERKWEYEQDDSKALQDVIDYIDRD
                     IPVLIQTDIFYLDYYNSSTHFPGHIVVVSGYDAQKQEVYLSDTGFEGLQAVSFDNFKK
                     SRSAKIKPYPLSNNWISVGGINTQNDLNELIPLAIKSNALKMLKGVKSPRGVSGVERI
                     LELSKDIFNWKDAADWKWSFRYSYQVIQKRGTCGAGFRWMYRDYLNEAKNYFDFEFTE
                     QIAIKMDHIGNEWYELSRFFKKISEEEKPDSFLAESSQRLFNIYTLEKNFYSEIAGYL
                     QIL"
     gene            12287..12958
                     /locus_tag="AC159U_09970"
     CDS             12287..12958
                     /locus_tag="AC159U_09970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3337419.1"
                     /translation="MNLLLFDIDGTLISTGGAALRAADRAFENIYGVKNVMANITADG
                     KTDYLILNEMFQNGLGRGYTEKESELIFNEYLTILDNNLNNGASVTVLPGVTNLLDEI
                     TDRDDTVIGLATGNIEQGAWIKLRHCGLDKYFSFGGFGSDAEHRATLVSAGIERGRRV
                     ADKHLEKILVIGDTPLDITHGRAAGALTVGVCTGRYSCDELGAHSPDYLFSDLTDTHS
                     LIDIL"
     gene            12985..13953
                     /locus_tag="AC159U_09975"
     CDS             12985..13953
                     /locus_tag="AC159U_09975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3337420.1"
                     /translation="MIEYNDGVHLKDTGIWIDATKKKNLSILSNPLSTGFLRHSKVIT
                     TPQTLKLLGKKLSSVSVLPCPYNHMFNLGNIDVELLPSGFILGSAQVLIYTDNKKILY
                     TNDFNLDVLSTSDPIEITQCDTLILKCAYGKKKYFFPSPEIAVNAIADFIDDCFENDL
                     VPVLLAEPLGNSQELVNLLGERGYKVIVHDSIYKNLEIYRSLGINFTKYKRLKKDSDL
                     DSKVLIIPPEQSKKDRFAKMENAVFAGVSELAVEPEIVKSSLGVEHAYPFSIRAGYDE
                     MLKLVELVRPKEVHLTGSTNVEFAADLKKKGINAKALQDPQQLRLI"
     gene            13963..14382
                     /locus_tag="AC159U_09980"
     CDS             13963..14382
                     /locus_tag="AC159U_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3337421.1"
                     /translation="MAVNTFGIELNTRGECDMIDITGYVEQYVTESELDCGTATVFVP
                     GSTAGITTIEYESGAITDFKAAIERLAPTDIHYNHDARWGDGNGYSHVRAALLGPSLS
                     VPFNSGRLLLGTWQQIVVVDFDNRPRNRKIVVQVIGD"
     gene            complement(14393..>14726)
                     /locus_tag="AC159U_09985"
     CDS             complement(14393..>14726)
                     /locus_tag="AC159U_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3337422.1"
                     /translation="TFLFNPPAQGSSALEIKEKLPETAKLVSAYHNLPAKELAKIDED
                     LDYDVVICGDDDDAKEVVKKLTEEMKNLRVLDAGALETSAMIEAMTPLIVNLNIRYKP
                     QHFSVKFV"
BASE COUNT         4563 a         2718 c         3221 g         4224 t
ORIGIN      
        1 tatgatacaa gcggcccgta taccgatccc gaagtagata ttgatgtact aaaaggtctt
       61 gatcccataa gagctggatg gatacaagag agaggagatg tggagcagct agatgatttt
      121 agctctgaat tttccagaaa ctctgcaaaa aatggaaacg gaataacatt caaaaacctt
      181 agaaaaccac ttagggccaa aaaaggcagc aatgtaaccc agatgcacta tgcacgtaag
      241 ggaatagtaa cccctgaaat ggaatacatt gctataaggg aaaatcagca gatagacagg
      301 ctaggtgaaa aatataaaga acttgccgca tatcatcccg gagagaagtt tggtgcaggg
      361 atgcccgatt catatataac tccggaattc gtaagagacg aagtagccaa gggcagggca
      421 ataattccaa acaacatcaa tcaccccgag tcagaaccaa tgattatcgg acgaaatttt
      481 cttgtaaaga taaacgcaaa tataggtaat tctgcagtaa gctccagcat agaagaagaa
      541 gtagaaaagg cagtatgggc atgcaggtgg ggtgccgata ccgtgatgga cctttctaca
      601 ggtgacaata tccatgaaac aagggagtgg ataataagaa attctcctgt tccgattggc
      661 acggttccaa tttatcaggc gctagaaaaa gttggtggca aagctgaaga acttaactgg
      721 gagatttttc gtgacacgtt aattgaacag gctgagcagg gggtggatta tttcactatt
      781 catgcgggag tgcttctaag atatattcca cttacagccg gcagagtaac gggcatagta
      841 tcaagaggcg gctcaataat ggcaaagtgg tgtctttctc accataagga gagcttctta
      901 tacactaatt ttccaaaaat atgcaaaatc atgaaagatt atgacattgc attttcactg
      961 ggtgacggtc ttagaccggg atgcattgct gatgcaaatg atgctgcaca gtttgccgag
     1021 cttaaaacaa taggtgagct tacaaaaaca gcatgggaaa atgatgtgca ggttatgatc
     1081 gagggtcccg gtcacgtacc tatgcatatg ataaaggaaa atatggacaa gcaattagaa
     1141 tggtgcgatg aggctccgtt ctatacactt gggcctctta caacagacat agctccggga
     1201 tatgatcata ttacatctgc aataggtgct gcaatgatag ggtggtatgg cacggcgatg
     1261 ctttgttatg taaccccgaa agaacacctt ggacttccta ataaaaaaga tgtaaaagag
     1321 ggtgtgattg catacaagat tgcagctcat gcggccgatc ttgctaaagg tcatcctact
     1381 gcacagtttc gtgacaatgt gctgagtaaa tccagattcg attttcagtg ggaagaccag
     1441 tttaatctct ctctcgatcc tgatactgcc aaagagtttc atgatgaaac cctcccttct
     1501 ccaaatgcca aagtggctca cttctgctct atgtgcgggc caaaattctg ctctatgaag
     1561 ataacacaag aggttaggga ttatgccaag gagtatcacc taaccgatat agatgcctta
     1621 gaaaaaggta tggaagagaa atcagatgag ttttccagta agggtggaga gatttatctg
     1681 gagcctgtag actgattatt ttgcttctac agcatacaac cactacattt ttccggttaa
     1741 tttacttggc tattcaggat atttctaatc gatttatata acatttctat tgctacatat
     1801 tcaatattta ggtatacgta atagattttt taataacttt gaggtaacca atggatttac
     1861 aacagattgc agatgaagtt tcgaaaagca ctaatataaa tgtaagcaaa actgatatag
     1921 agcgtttggt aagcatttta agaaaaaaaa ccaacccatg ggagattatt gaccgctgcg
     1981 attttcctat tcctgctgtg tttgaatcga ttgaaaggct aaatagcacg gatattgtaa
     2041 atattacgga taaaggtatt gagcttacaa ataaaggaag ggaatcggct agcgagctgc
     2101 acccggttga agacttatcg tgtaaaacat gcgaaggcag agggattgac cttaaaaaat
     2161 ttgctcctgt aagggaaaaa tttatacagc tgcagcgtaa aagaccaaag ccgtcccatg
     2221 attatgatca gggatacgtt acaccccatt ctacattttc aaggtttttg gcagcatatg
     2281 aaaggggtga tatatacgca aaggatatac ttatcattgg tgatgatgat ctgcttagca
     2341 tcgtactggg gctaagtggt atgccaaaga gcatcactgt gttggagata gacaagaggc
     2401 ttacaaagtt tattgctcaa attgcagaac aggaaaaatt tgaggttaac atcgaaccat
     2461 ttgatatgag aaaaccactt cccaaaaaat atattaagag ttttgataca ttttttaccg
     2521 accccccaga gactttaaaa gcagcagatg catttgttgg taagggtatt agcactctca
     2581 gatcggcagg gtgttcagga tattttggat ttacgagaag agaagcatcg ctaaataagt
     2641 ggtatgacct gcagaaactg ctcacaagct acaaggtagt agtaaccgat ataatgcaca
     2701 actttaacga gtatattaac tggggctatg aaaaggaaac aagagcctgg gagctttcac
     2761 cccttaaaat aaagcctaag aaaaattggt acagatctgc tttttacaga attgttacac
     2821 ttaagggata taaaggaagc agcaaaaact acggtacaca aaatatctat gaagatatag
     2881 agagttcaac aacctgactt taatatgcca aagagaaccg atataaagaa aatattgctt
     2941 ataggatcag gccccattgt gattggacag gcctgtgaat ttgattattc cggtactcag
     3001 gcgtgtaagg cgctaaaaga agagggtttt gagatagtgc ttgtaaacag caatccggcc
     3061 actattatga cagacccctc aatctctgat aaaacctaca tagagccgct aatccccgaa
     3121 atacttgaaa aaattattga aaaagaaaga ccgcaggcat tgcttcctac gatcggaggt
     3181 cagacgggtc ttaaccttgc cgttagtctt gccgagagcg gggtgcttga taaatacgga
     3241 gtcgagctta tcggagcaaa ccttgaggct ataaagaaag cagagaacag ggataaattt
     3301 aaagatgcca tgataagcat agggcttcag gtcccagaaa gcggagtagc aagaaccatg
     3361 gaacaggcct gggaaattgt gaagagggtt ggctatcccg taattatccg cccgtcattt
     3421 acccttggtg gggtaggcgg cagtattgca tataataaag aagagtttga agatttttca
     3481 aaagcgggtc ttcatatgag ccctgtgacg gagatactgg tagagcagtc ggttatagga
     3541 tggaaagagt ttgagcttga ggtaatgaga gactctgctg acaatgttgt aatcgtatgt
     3601 acaatcgaaa actttgaccc catgggtgtg cacaccggag acagcataac agtagctcct
     3661 tcgcaaacac ttacagataa agaatatcag aggctgagag atgcttctat agcgatcata
     3721 agagagattg gagtagatac cgggggctca aacattcagt ttggtataaa tcctgaaaat
     3781 ggtgatattg tggtaataga aatgaaccca agggtttcaa gaagttcggc tcttgcctca
     3841 aaggctacgg gatttccaat tgcaaagatt gctgcaaaac ttgcagtagg atatacactc
     3901 gatgagatag caaatgatat cacaaaatat acccctgctt catttgaacc aagtatagat
     3961 tatgtagtag ttaaaatacc aaggtttgca tttgagaagt ttccacagac tgagcccctt
     4021 cttactaccc agatgaaatc agtgggagaa gttatggcaa taggaaggac atttaaggaa
     4081 tcaatgcaaa aggcaatacg ttctcttgag attgattcag ccgggtttga aagcaaaacg
     4141 gatgatcttg gcataattaa agataaactc agaaagccaa accccgacag gctatggtac
     4201 ctggccgatg cattcagggc tggattggac ttggaagaaa tttttgattt aacctatatt
     4261 gatccgtggt tcttgcagaa tataaaacag atagtggatg cagagaccga tctgatcaag
     4321 tcttctaaag atggaggggt ttcggtagat gatttcaggc ttttaaaaca atacggattt
     4381 tcagatgaga gacttggctt tctacttggc aaaagtgaag atgaaattag aaatactaat
     4441 cttgataatg gtataaagtg tgtttataaa atggttgata catgtgctgc ggaatttgaa
     4501 gcgcataccc cttatctgta ctcttcctat gaacaggaag acgaagccct tccaactgac
     4561 aggaaaaaag taattatttt aggcggaggt ccaaacagaa tagggcaggg aatagagttt
     4621 gattactgct gtgtgcatgc ggcgttttct ttaaaagaag agggctatga agccattatg
     4681 ataaactgta atcccgagac tgtaagcact gactacgata cttcggacag actttatttt
     4741 gagccgctta cactcgaaga tactctggca attattaata gagaaaagcc cgagggggta
     4801 atagttcact taggaggaca gacacctctt aagcttgctg tgccactgga gcaaaacggc
     4861 gtaaagataa taggcacttc cccggataat attgatcttg ctgaagaccg ggaaaggttc
     4921 agagacctga ttttgaaaat caagctcaag caaccccgaa gcggtattgc aaggagtgaa
     4981 gaagaggcgc ttgcagttgc cggtgaaata ggatatccga ttgtagtaag gccgtcttat
     5041 gtgcttgggg gcagagcgat ggagatagtg tatacggaaa aatctctcag gcgatatata
     5101 aaagatgccg tgaaagtatc acacaaccat ccggtgttga tagacgaatt cttaaaagat
     5161 gcaaaagaag tagatgtaga cgccatatct gacggcaaga cagtagtagt cggcggtata
     5221 ctggaacata tagaagaggc aggagttcat tctggtgaca gcgctatggt gcttccacct
     5281 tactcgcttg ataccgaaat agtcgatgaa ataaaaaggc agacaaaaga gcttgcactt
     5341 gcactgaatg tgattggtct ttgtaatatc cagtttgcag taaaagacgg agaggtatac
     5401 ttaatagagg tgaacccccg tgcgagccgt actatccctt ttgtaagtaa agccataggt
     5461 gttccccttg caaatctggg aacaaaagtg atgacaggca agacactaaa agagcttgga
     5521 tttactaaag agataacacc caagcatata tgcgtaaaag aatcagtgtt tccattcata
     5581 aggttttccg gggttgatac tatcctgggc cctgagatga aatccacagg tgaagtgatg
     5641 ggcattgata cagatcttag aaagacattt gccaaggcgc ttcttgctgc aggaagtgag
     5701 cttaaaaccg agggtagcgc ttttattagc gtgaaggatg acgacaaaga taaaatgctc
     5761 cctgttgcaa agcatttggt ggaaattggt tataagctaa tcgccacaaa aggtactcat
     5821 aaatttttaa agaaaaacgg tatagaatgt gaaagcgtaa agaaggtata cgaaggcagg
     5881 cctaatattg ttgattttat tattaatggt gatgtgcagc ttgttatcaa taccacattt
     5941 ggagaagacg agattaagca gtcatattcc ataagaagag agtcacttat gggaaatgtg
     6001 ccttacttta caactatttc gggagcaggg gctgctgttg aagcaattga tgctcttaaa
     6061 aatgaaggtc ttgatgtaaa gccgattcag gattattatc ttgatagcaa cctaaattag
     6121 taaatctcaa atgcataaat atgtataggc ctgtctggag gaaatatgtc tattgaactt
     6181 gtaccgatta cacctgacgg atatgaaaaa ttaaaaaaag agcttgaaaa tttaatcagg
     6241 gtggaaagac ccagtgttat aaaagcaata gaagaagcca gagcgtttgg agacctctca
     6301 gaaaatgccg aatatgatgc agccaaagaa aagcaaagtt ttgttgaagg aagaattcag
     6361 gagcttgaga gtaaattatc cagagcagaa gttattgatc ctaaatcaat cacaaacaaa
     6421 gaaagagtta cttttggctt aacagttaaa atggaagacc tggatacagg tgaagaagtt
     6481 acctatcagc tcgtaggccc agatgaaaca gagcccgaga acggaaagat ttccataacc
     6541 tccccaatag gaagagcaat gctcggaaaa caggtggatg aagatgttac tgttactact
     6601 cccggtggta ttagagagtt tgaggtgctt gatatacttt aggttgtaga atttattctt
     6661 gtttttctat ctattaattc aatttaactt agtcaaaata actccgcctg ctttgtaggt
     6721 tttggaaggt taagatgttc ataggcattc aaggtaactg ttcttcctct cggagttttt
     6781 tgtaacagcc cctgtctgat aagatatggc tcatatacat cttcaatcgt gtccttgtct
     6841 tcactcacag ctgctgcaat ggtgtctata cccacagggc ctccgccaaa cttttctata
     6901 atggataaaa gaattatccg gtctatctta tcaagtccga tagaatctac ttcaaacatc
     6961 tcaagtgctt cttttgctac ctttatatca atttccccgt ctgcctttac gtctgcatag
     7021 tctcttactc ttttaaggag cctgtttatt atgcgtggag tgcccctgcc gcggcaggca
     7081 atctcttttg ttccttcgct gtttatttta acttccagaa tattggcaga gcgtttaata
     7141 atatcttcaa gttcatcagg ctcatagtaa tcaagcctga ggtcaagccc gaagcgcgac
     7201 cggaggggtg aggttatgag ccctgttctg gttgtagctc caattagcgt gaaatggttc
     7261 acatttatct tcattgatct tgctgatggc ccttcgccaa tcataagatc gatcttgaaa
     7321 tcttccattg cagaatagag gtactcttct acaattctgt ttagacggtg aatctcgtct
     7381 ataaagagca cgtcaccttc ggacaggttt gtaagaatag aagcaaggtc tccaactctt
     7441 tcaaccactg gccctgaggt tgcgtgaatt tttaccccaa gctcatttgc tactatgtgt
     7501 gcaagtgtag tttttccaag ccctggcggg ccgtaaagca gcaggtggtc taatacttct
     7561 tttcttttcc ttgcagcctg tatgcatatt aaaattttct cttttgcatt tttttgaccc
     7621 aggtattcat ttaaaaacct tggcctcagg ttgttgtcaa agctgctgtc atcagcattt
     7681 tcttctggcg ctattaatct ttgttccatt ctatacctgt ttcattattt tgagtgattc
     7741 ctttattact acttcaatgt tgctgttatc atttataagc tcttttatag tgtgcaaatt
     7801 ttcatcgatc tcatttggtc tgtatccaag attgctaagt actgatatta catcgtcaag
     7861 atcattatca ccactttttg ctgcacccac aggagttttt atatcaagca gttttatctt
     7921 atcttttagc tcggtagtaa ttcttgcggc aagtttgggt ccgatgccgg ggattttctt
     7981 tttggcaagg tttcctgagg atatttctgc aacaaggtca tgcggcgaaa tatgggagag
     8041 aatatttacc gaagcccttg ggccaattcc tgaaacgcca agaagtatct tgaaaacctc
     8101 tttttcctcc cctgagagaa aaccgaacag ttcgagcgtg ttttctttca tatgagtgta
     8161 aatatgaagc tctactgaat ttccaaactc aggaagctgg aaataagtgg atgaaggcac
     8221 aagcactccg tagccaactc cccccacatc aacaattact tcttcagggg ctttctttgc
     8281 tattattcct ttaagaaggg atatcattta gagtaaatcg ttttttgctt ctggattttt
     8341 ttcttatcgt aaacccatat tttttcttta tttcaatgct gtttaaatga cagatcgcaa
     8401 tcgcaagggc atcggaagtg tcagattttt caaaatagtc aatattcagc atagtgctta
     8461 tcatcttttg cacatctaat tttgttgccc ttccgttacc tgtaactgct gattttacct
     8521 ctgttggaga atattcaaaa actggaatat ccgctgtagc tgctgcaagg attgatactc
     8581 ctctggcctc tccaagcttt agagtgctcc ttggattttt tgaaaaaaat acatcttcaa
     8641 tcgacatttc atcgggagag aactgttcaa ttaattctga aagcttgccg tatatatgtt
     8701 ttaatctact gggaattgac ctgctctgcg gctgacttat aaatcccgaa tcaatacagt
     8761 taaaaccgct gtcccgcact tctactattc catacccgct tacaatagtt ccaggatcta
     8821 tgcctataac tatcatgttg tgttactggc ctacggcttc tagcatctcg tcgggaatat
     8881 caaaattaga gtagacatcc tgcacatcat cgctgtcttc aagggcttcc attagcctta
     8941 gcagctgacc tgcttccttg ccctcaacct ttactacatt ttgaggaatc attgtgactt
     9001 ctgctgactc ttgttttagg cctgcctgct cgagtgcgga tcttacttct tcaaaaacac
     9061 caggctcagt aagaacagca aattcaaaat catcatctat aacgtcatcg gcccctgcat
     9121 cgagagcaag ttccataagc tcatcttcat taattgaaga tttgttaaaa gcaatccttc
     9181 ctttcttctc gaacatccag gcgacacaac ctgcttcacc catatttcca ccgtgtttgt
     9241 taaatatttt tcttacttca gacacggttc tgtttttatt gtcagtaact gcgtttacaa
     9301 gcacagccgt tccccctggt ccgtatcctt catatgacag ctcgtgaagt tcatctaccc
     9361 cagcctcgcc ggtgcctctt ttgatggccc tgtcaattgt atcgttaggc atgttctgtc
     9421 ccttggctgt gttaattgct gaccggagtc ttgggtttgc ctctgggtca ccaccaccag
     9481 ctcttgcagc gacagttagc tctcgtatga gttttgtaaa aaccttacct cttttagcat
     9541 caaccgatgc ttttttgtgt tttatattag cccatttgga atgacccgac atatagcacc
     9601 acctcttacg attaaattat tttaagggaa ctacttacag atatatttaa tacattttat
     9661 agtaattact gtactatagc aaggttttct ttgctatttt tattacttaa atattactgt
     9721 ataataatag tgattttcat taaatttaat tatagcatta attattcttg acaaatatac
     9781 tttcttaggt gcataatatt aatgttacaa atcaattatt aaattaacta tttaccgaat
     9841 tcaatttttt ccacagattt ttttaaaaac tatttttcta tattaattct ataattctat
     9901 ggcaagagta tccaaagcag aacgtacttc aaacggatca tccccagatg gtggtccatt
     9961 acttaattta aatgagctta ggaatagaaa gtccaaagag cttatgacaa tagcaaggga
    10021 gcttgatatt gaagaagctc caagaatgtc aaagcaggat cttatctatg cagtacttag
    10081 ggcacaaagc gcaaaggaag gatctataat cgcagaaggc gtgcttgaga tcctttcaga
    10141 gggatatggt tttctaaggt ctccaaaata cagctaccta ccgggtccgg atgatattta
    10201 tgtatctaaa tcacagattc gttcatttaa tttaaaaaca ggagatacaa ttggcggaca
    10261 ggtaaggctt ccaagagaag gcgagaaaaa tttagcacta ttaaagattg aaaccgtaaa
    10321 ccatgaatct ccagaggttt gcagagaaag ggttgcattt gagaacaggg ttcctctgca
    10381 tccggaagaa aaactaaatc ttgaatttga taagagtgaa ttctgtacaa gggttatgga
    10441 gctttttgtg ccggtaggta agggacagag gggattaatt gttgctccgc caagaactgg
    10501 taagacaatc cttatgcagc atatagccaa tgccattacc aaaaacaatc ccgaagtggt
    10561 tctcatcgtt ttacttatag atgaaagacc tgaagaagta acggatatgg aaaggtctgt
    10621 aaatggagaa gtcataagct caacttttga tgaaccatca caaagacata ttcaggtatc
    10681 agacatggcg cttgaaaaag ctaaaagact tgtagagcat ggaaaagatg tagtcattct
    10741 gctagatagt cttacccgtc ttgcaagggc aagtaacact gttacaccag caagcggaag
    10801 agttctttcg ggtggtatgg aggcaaatgc tctgcaaaga cctaagaggt tttttggagc
    10861 agccagaaat actgaagaag gtggaagctt gaccatcatt gctacggctc taattgatac
    10921 cggcagcaga atggatgagg ttatctttga agagttcaag ggtactggaa acatggaagt
    10981 ttatctggac aggaaacttg cagataagag aatatttcct gctattgatc tacagcgctc
    11041 cggaaccaga aaagaagagc ttcttcttaa agaagacgta cttacaaggg tgtggctgct
    11101 tagaaaagta cttagcccaa tgaacacagt ggaagcaatg gaattcttgc tggataagat
    11161 aagaggtaca aagtccaata aagaattcct taacatgatg aatcagtagt catacaaaac
    11221 tgatttcact caaatcattt ctttttaaat ccaaacaata tctattctat gaagctttga
    11281 aaaaagttat caagggttgg aatcacatac cgggcattca ctgcggatcg gcagcgctaa
    11341 gagatgttgc cactttttat ggactgcctc ttagcgaacc aatgtgcttt gggcttggag
    11401 ggggtcttgg atttttttat tccatagata atgagataag tcctacgagg aacatccacc
    11461 taagagggcc cgatatggag cctggcttct tttcactttt tactacagaa gaaagaaaat
    11521 gggaatatga gcaggatgac agtaaggccc tgcaggatgt gattgattat attgacagag
    11581 atatccctgt attaatacaa acagatattt tttatcttga ttactataat tccagcacgc
    11641 atttcccggg acacattgtt gttgtaagcg gttatgatgc tcaaaaacaa gaagtctatc
    11701 tgtctgatac gggttttgaa ggtctgcagg ccgtatcgtt tgataatttt aaaaaatcgc
    11761 gtagtgcaaa gataaagccc tatccactta gtaacaactg gatttcagta ggtggtataa
    11821 atacgcaaaa tgatctcaat gaattaattc ccttggcaat taaatccaat gcgctgaaaa
    11881 tgttaaaagg ggttaaatct ccaaggggag tatccggtgt agaaaggata ttagagttat
    11941 caaaagatat atttaactgg aaagatgctg ctgactggaa gtggagtttc aggtattcat
    12001 atcaggtaat tcaaaaaaga ggcacatgcg gggctggttt taggtggatg tacagggatt
    12061 atctaaatga ggctaagaat tacttcgatt ttgaatttac tgagcagatt gcaattaaga
    12121 tggatcatat aggaaatgag tggtatgaac tatcaagatt ttttaaaaag ataagcgaag
    12181 aggaaaaacc tgatagcttt ttagcagaat cttcacaaag gttatttaat atttatactc
    12241 ttgaaaaaaa tttttattcc gagattgcag gttatcttca aatactatga atttactact
    12301 ttttgatatt gacggaactc taataagtac aggaggtgct gcgcttagag cggccgaccg
    12361 ggcatttgaa aatatttacg gtgttaagaa tgttatggct aatattacag cagacggtaa
    12421 aactgactat ctcatattaa atgaaatgtt ccaaaacggc cttggaagag gttatacaga
    12481 aaaggagtca gagcttatat ttaatgaata tctaacaata cttgataata atcttaataa
    12541 cggcgcaagc gtcaccgtac ttcccggtgt aaccaatcta ttggatgaaa ttacggatag
    12601 agatgacacc gtgattggtc ttgcaacagg caacattgaa cagggagcat ggataaaact
    12661 caggcactgc ggcctggata agtatttttc ctttggcggt tttggttcgg atgcagagca
    12721 cagggctacg cttgtgagcg ctggaataga acggggtaga agggtagctg acaagcacct
    12781 tgaaaagatc cttgtaattg gtgatacgcc gcttgatata acgcatggaa gggctgcggg
    12841 agcacttacg gtaggtgtat gcaccggcag atatagttgt gatgaacttg gcgcgcatag
    12901 tcccgattat ttgttctctg atttaacaga tactcattca ttaattgata ttctttaatt
    12961 tacctgtttt attacaggtt atatatgatt gagtataatg acggagtaca tttaaaagat
    13021 acgggtatat ggattgatgc tacaaagaaa aagaaccttt caattctttc caatccgctt
    13081 tcaacagggt ttttacggca ttccaaggta ataacaactc ctcagacact aaagcttttg
    13141 ggaaaaaagc tctcttctgt aagcgtacta ccctgcccgt acaaccatat gtttaacctt
    13201 ggaaatattg atgttgagct tttgccttcc ggttttattc ttggttctgc acaggtactc
    13261 atttacacag acaacaaaaa gattttgtat actaacgatt ttaatcttga tgttctcagc
    13321 acatctgatc ctatcgagat tacacagtgt gatacgctta tattgaaatg tgcttacggt
    13381 aaaaagaaat attttttccc aagtcccgag attgcggtaa atgccatagc ggactttatt
    13441 gacgattgtt ttgaaaatga ccttgttcct gttttgcttg cagagcctct tggaaattca
    13501 caggaacttg taaacctgct gggtgagcga gggtacaagg tgattgtaca cgactctatc
    13561 tataaaaatt tagagatata cagatcattg ggtattaatt ttacaaaata taaaagactt
    13621 aaaaaagaca gtgaccttga ttcaaaggta ttaataatac caccggaaca aagcaaaaaa
    13681 gacaggtttg caaagatgga aaatgctgtt tttgccgggg tgtccgagct tgctgtggag
    13741 cctgagatag taaagtcttc tttgggtgta gagcatgctt atccgtttag tataagagcc
    13801 ggatatgacg agatgctaaa gctcgtggag cttgtaagac caaaagaggt gcatcttaca
    13861 ggaagcacaa atgttgagtt tgcagccgat ctaaagaaaa agggtataaa tgccaaagcg
    13921 cttcaggacc ctcagcagtt aaggcttatt taggagaatc agatggcagt aaatacattt
    13981 ggaattgagc taaatacaag gggagagtgt gatatgattg atatcacagg atatgtggaa
    14041 cagtatgtaa cagagtcgga actggattgt ggcacagcta ccgttttcgt tccgggatca
    14101 actgccggta ttacaaccat tgaatacgaa agcggtgcaa taactgattt taaagcagca
    14161 attgagaggc ttgcaccaac tgatattcac tataaccatg atgccaggtg gggtgatggt
    14221 aacggctact cacacgtacg cgcggcactt ctaggcccgt cactatccgt accttttaat
    14281 tctggcagac tcctcctggg cacatggcag caaatagttg ttgtggattt tgataacaga
    14341 ccccggaaca gaaaaatagt agttcaggtt atcggggact gattcttaaa tcctatacaa
    14401 acttaacaga aaaatgctga ggtttgtatc tgatgtttag gtttacaatg agaggggtca
    14461 tggcttcaat cattgctgat gtttccaaag cccctgcatc gagaactctt aggttcttca
    14521 tttcctcagt aagtttcttt accacttctt ttgcgtcgtc atcatcaccg catattacca
    14581 cgtcgtaatc caggtcttca tcaatctttg caagctcctt tgcaggaagg ttatgataag
    14641 cagaaacaag ctttgctgtt tcaggaagct tctcttttat ttcaagagcc gatgaaccct
    14701 gagctggagg attgaataaa aaagtc
//
LOCUS       DBNGSV010000268         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_741446, whole genome shotgun sequence.
ACCESSION   DBNGSV010000268 DBNGSV010000000
VERSION     DBNGSV010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_741446"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1589
                     /locus_tag="AC159U_09990"
     CDS             join(242..564,563..1589)
                     /locus_tag="AC159U_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611539.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3337423.1"
                     /translation="MAESKNVIKQIRKATRRQFSADEKIRIVLEGLRGETSISEICRR
                     EGIAASVYYKWSKSFLEAGKNGLTRTILRDATSEEVRGLKQENSDLKKAVAELVLEVQ
                     RYKKSLSACKRRRYQRMSRNEKMKFIRLIEGSELCISDALAKYDVPRSTYYRWKRKIR
                     SQGINGLADNKSYRARTWNQLLPWQIDKIIEYAMFNPEYSSREISLYITDNEGFSVSE
                     STVYRRLKERGLIPEPKIKRFPASDEYHTKTNVINQLWQTDATYLKVDRWGWFYLISV
                     LDDYSRKILAWQLRTSMKAEDFSDVVEQACAFTGLDNVPVENRTKLLSDNGSALVGKD
                     FGDYLEARGIGHIFASPYHPQTNGKIERYHRSIKEQIFLHVWELPEELEKEIARFVSW
                     YNNRRYHEALGNVTPNDVYFGRREEILEKRKQLKNKTILERKRINCKIIETGVEIAS"
BASE COUNT          534 a          306 c          392 g          436 t
ORIGIN      
        1 attcactatg atttatgggc ggttctatca tggttgccgg ggcttcatac agcttcgcat
       61 gatgtatata ttggtaagaa ttttgacgat gtaaactgtt tcccgtcagc agaagtggca
      121 caggctaatg cctttggtta ggggagacct tcaagtgttt tcctacatgc aggaaaacac
      181 ttgaaatcgc agatttcagc tccataatcg gggtcctttg atcccggaaa ggagttgaaa
      241 agtggcagaa tcaaaaaacg taataaaaca gatcagaaag gcaacccgcc gacaattctc
      301 tgcagatgag aaaatccgaa tagtcctcga agggctacgg ggtgaaactt ccatatcaga
      361 aatttgcaga cgagaaggca tagcagcttc agtttactac aaatggtcta aatcattcct
      421 cgaggctggc aaaaacggtc ttaccaggac tatactccgc gacgccacca gcgaagaggt
      481 acgtgggctt aagcaggaaa atagtgatct gaagaaggct gtagctgagt tagttcttga
      541 ggttcagcgg tataaaaaaa gtctcggcat gtaagcgaag gagatatcag cggatgagta
      601 gaaacgaaaa gatgaaattt atacgcctta tcgaaggatc tgaactttgc atcagtgatg
      661 ccttggccaa atatgatgtt ccaagaagca cttattaccg ctggaagcga aaaattcgca
      721 gtcaaggtat aaatggcctt gctgataaca aatcctacag agcaagaacc tggaatcagt
      781 tgctaccgtg gcagattgat aaaattattg agtatgcaat gttcaatcct gaatattcca
      841 gcagagagat aagtctatat attactgata acgaaggatt tagtgtctct gagtctacgg
      901 tttacagaag gcttaaagaa cgtggattaa ttcctgagcc aaagattaaa agattccctg
      961 catcagatga atatcataca aagacaaacg tcatcaatca gctctggcag acagatgcta
     1021 catatctaaa ggttgaccgt tggggctggt tttatctgat tagtgttctt gatgattaca
     1081 gtagaaagat ccttgcatgg cagcttagga cttctatgaa agccgaggac ttcagcgatg
     1141 tggtagaaca ggcctgtgct tttacaggtc tggataatgt ccctgttgag aatcgcacaa
     1201 agctgcttag tgataatggt tcagcccttg tcggaaaaga ctttggcgat tacctggagg
     1261 caagaggtat aggccatata tttgcatcac cttatcatcc acagactaac ggcaagattg
     1321 agcgttatca ccgctcaata aaggaacaaa tatttttgca tgtttgggag cttccggaag
     1381 aactcgaaaa ggaaatcgcc aggttcgtta gctggtataa taaccgcagg tatcatgagg
     1441 ctctcggcaa tgttacacct aatgatgttt actttggcag gcgggaagag atattggaaa
     1501 agcgaaaaca gttgaaaaac aaaacaattc ttgaaaggaa aagaattaat tgtaaaatca
     1561 ttgaaaccgg agttgagatc gcctcctaat ttaaaggtaa atctgtctta tttttgctga
     1621 agacgtacat gataaagcat cttgatcttt tggcatgaaa cagtaaat
//
LOCUS       DBNGSV010000269         1540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1363838, whole genome shotgun sequence.
ACCESSION   DBNGSV010000269 DBNGSV010000000
VERSION     DBNGSV010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1363838"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="AC159U_09995"
     CDS             complement(<1..1057)
                     /locus_tag="AC159U_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004759867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3337424.1"
                     /translation="MHKSLLEILLKERILILDGAMGTMIQRYKLEEEDFRGNLLRDHH
                     SDLKGNNDLLSLTRPDIISEIHRQYFEAGADIVETNTFSATTIAQADYGLQHIVKDIN
                     RESAKLAKNVASEIMNEYPDRKCFVAGAIGPTNRTASMSPDVNNPAFRAVSFQELVDA
                     YTEQVEALVEGGVDILLPETTFDTLNLKAAIYAIEQFFDKSDKRLPVMLSVTITDKSG
                     RTLSGQTIEAFWNSVRHAKPLSVGINCALGAEDMRPYIEELSNIADCYISCYPNAGLP
                     NPLSETGYDQTPEITSTLLDEFCSSGFINIVGGCCGTTPDHIGAIAKKVKNYKPRQVP
                     RIEQATRLSGLEALSIGS"
BASE COUNT          489 a          279 c          230 g          542 t
ORIGIN      
        1 tagatccgat actcaaagct tccaggccgc ttagccttgt cgcttgttct atccggggaa
       61 cttgtctagg tttatagttt ttaacttttt ttgcaatggc accaatatga tcgggtgtag
      121 tcccgcaaca tccgcctact atattaataa aaccactact acaaaattca tccaataatg
      181 ttgaagttat ttcaggggtt tgatcataac ccgtttcact aagaggattt ggaagcccgg
      241 catttgggta acagctaata taacaatctg caatatttga tagttcttca atataagggc
      301 gcatgtcttc ggctccaagt gcacagttta ttccaacact aagcggcttt gcatgtctaa
      361 cagagttcca aaatgcttca attgtctgtc cggaaagtgt tctccctgat ttatcggtaa
      421 ttgttactga tagcatcacg ggtagtctct tatcggattt atcaaagaac tgctcaatag
      481 catatattgc agcttttaaa ttcagtgtat caaaagtagt ctctggaaga agtatatcca
      541 ccccgccctc aacaagagct tcaacctgct ctgtatatgc atctacaagc tcctggaaac
      601 tcactgccct gaatgccgga ttatttacat ctggtgacat tgaagctgtt ctatttgttg
      661 gtcctattgc ccctgctaca aaacatttgc gatctggata ttcgttcatg atttcactgg
      721 caacattttt ggctaatttt gcagattctc tatttatatc cttaactatg tgctgtaatc
      781 cgtaatctgc ctgagctatg gttgtggcac taaaagtatt agtttcaaca atatctgcac
      841 ctgcttcaaa atattgtctg tgaatttctg agataatatc tggtcgtgta agtgaaagta
      901 gatcattatt gcctttaagg tctgaatgat ggtctcttaa caaattgccc ctaaagtctt
      961 cttcttccag tttgtatctc tgtatcatgg tgcccatggc accatctaga attaagattc
     1021 tttcttttaa taatatttcc aacagactct tatgcatatt attattatct tttatatatc
     1081 cttctatgtt gatcaataaa tattgtttat tgataaaata ttaatatctt gttataacaa
     1141 actgacacta atatttatga tttttcaaca agtttgttga atattagtaa tattggacta
     1201 ataattgtta atattcatca cttaacatca tcttatcaat ttataatcta caaataatta
     1261 tatttttatg aaaaaataca atattataat acaggtagat aaatcaatct cagataactt
     1321 aaaatcatca ttagagacta aatcatattg ttcctgcggt tctcaatata gatgatgata
     1381 cttcaaaatc aataaaaaaa tatagtcctg atctattaat agtagatatt ggcttaaaag
     1441 ataacattag taaatcgaag aaattacaaa aatatgtcat tccatacatc tatttaagta
     1501 acaataaaac aaataaatta aaagaatata atattttaac
//
LOCUS       DBNGSV010000270         2887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1019288, whole genome shotgun sequence.
ACCESSION   DBNGSV010000270 DBNGSV010000000
VERSION     DBNGSV010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1019288"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      185..273
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            403..1520
                     /locus_tag="AC159U_10000"
     CDS             join(403..664,664..1520)
                     /locus_tag="AC159U_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558178.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3337425.1"
                     /translation="MKKSRFSESQIVAILKEADAGVKVGDIWRKYNISSATYYKWKSK
                     YGGMEVSDVKRLKELEAENTRLKQMYADLSLENHALKDLIEKKPIEPPDKRDAVGFLV
                     AKHKLSVSKACGCMSLSKAAYYKQGVDWQKRDAPVIKALNTIVEQHGRWGFWKCYRRI
                     RLDGYFWNHKRVYRVYRAMGLNLKRRTKKRVPVRKRQGMEVPNIPNMIWSLDFMNDAL
                     YSGRRFRTLNVLDEGVREALNIVVDTSLPAARLVRVMEEIKEWRGLPMAIRCDNGPEL
                     TSGSFVDWCTEHNIEILYIQPGKPAQNAYIERFNRTFREEVLDAYLFENLEQAQTIAN
                     DWIKDYNERRPHESLGGLPPSMFRKMVMSEGSTLQLST"
     gene            1537..2793
                     /locus_tag="AC159U_10005"
     CDS             1537..2793
                     /locus_tag="AC159U_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337426.1"
                     /translation="MNNDVSSNFTYKERENYNNFFLLKLRYSLILILALFTFLSFQSE
                     VNAALTDAATDWSNFTFFPVREPVSGEPLSDYEGGAADPTKGQASPGGNGAFASDCNA
                     GGNAGTSGCSEALCVPNLMTGDRGCGGATSFFWACYDGGDGSTGSARTNDVFAFRLRI
                     DGSPIQANDADLLTNGKYNILLDLDGDGFKEFWILVDGNADNMLVLHEDGAPGTQAAD
                     EVADLVDTFSACSDEATITCQSGGMDTSHTRTSQLAGTIGAGSDTEWYIDIQVPASSL
                     CAVGVTCDSATQLADCDSNLGLFATTSDSNTDPLQKDLIAQCPDTNNSGGTDPGDECT
                     FSQSTPITLSYFSSSVGASGAVLNWSTATEVGNAGFNIYATTAQGLVQINDSIIPSKV
                     IDSMSPVDYSFAANASLSSPRIQVHL"
BASE COUNT          873 a          513 c          691 g          810 t
ORIGIN      
        1 gcacttccaa tacaaacatt tgtattagct tgcttatctt actatttgat agttttgtat
       61 tcttaagtaa cttcttttgc atagtttcag cttagtatac tttgctgtcc tatgcgagct
      121 ccaaaaataa tttaattgaa atagagagtt agaaatagaa atatagaaaa atagattaat
      181 ttttacaccg aaaaggcaca ctgtatgaaa atacaggccg caaagttaat acctaccggg
      241 tctttaaaac cccatggtcg aatgactgcc gaagattaat caattacaac atttgagtat
      301 taatactatt taataaaggg gaataagatt gtaagcttcc ccaaagggtg gacaagcaat
      361 aagcagaact ttctggcaaa ataacatagc caggaggttt atatgaagaa gtcaagattt
      421 agtgaatcac agatagtagc gatactaaaa gaggcagatg ccggggtaaa ggttggggac
      481 atttggcgta aatacaatat aagctcagct acatattaca aatggaagtc aaagtacgga
      541 ggcatggaag tctccgatgt taaaaggctc aaggagcttg aggctgaaaa caccaggtta
      601 aaacaaatgt atgctgatct cagtcttgag aatcatgcgc ttaaggacct gatagaaaaa
      661 aagctataga gccgcctgat aagagggatg cagtagggtt tctggtggct aaacacaagc
      721 ttagtgtgag taaggcctgc ggctgtatgt ctttatcaaa agccgcttac tacaaacaag
      781 gggtagactg gcagaagcgg gacgcgcccg tcataaaagc gcttaatact atagtggagc
      841 agcacggtcg gtgggggttt tggaaatgtt acagaaggat taggcttgat gggtattttt
      901 ggaatcataa acgcgtgtat agggtataca gggcaatggg actgaacttg aagcgtagaa
      961 ctaagaagcg agtgcctgta cgcaaacgcc agggcatgga ggtgcctaat attcccaata
     1021 tgatctggtc tttggatttt atgaatgatg ctttgtattc agggcgtcgc ttccgcaccc
     1081 tcaatgtgtt ggatgaagga gtaagggaag ctctgaatat agtggttgat acttcattgc
     1141 ctgctgcaag actggtaagg gtaatggaag agatcaagga gtggcggggg ctgcctatgg
     1201 caattcgctg tgacaatggc ccggagctga cttcaggtag ttttgtcgat tggtgtacag
     1261 agcacaatat tgagatactt tacatacagc cgggcaagcc agcgcagaat gcatatatag
     1321 aacgttttaa tagaacattc cgagaggagg tgttagatgc ttacttattt gaaaatcttg
     1381 agcaagcaca aacaatagcg aatgactgga ttaaagatta caatgaacgc cgtccccatg
     1441 agtcacttgg agggctgcca cctagtatgt tcaggaaaat ggtgatgtcc gaaggttcta
     1501 ctttacaact gtctacttaa tggggaagcc tacaagatga ataatgatgt gtcaagtaat
     1561 tttacttata aagaaagaga aaattacaat aatttctttt tattaaaact aagatattca
     1621 ctaattttaa tactagcgct atttacgttc ctgtcatttc aaagtgaagt gaatgcggca
     1681 ctcaccgatg ctgcaacaga ctggtcaaat tttacttttt tcccggtaag agaacccgtc
     1741 tctggtgaac cattaagtga ttatgagggt ggagcggctg atccaaccaa aggacaagct
     1801 tctcctggtg gaaatggtgc attcgcatct gattgtaatg ccgggggcaa tgccggtact
     1861 tcaggatgta gtgaggccct ttgtgttccc aatcttatga ctggagatag gggatgtggt
     1921 ggagcaactt catttttctg ggcttgttat gacggtggtg acggcagcac aggatcagca
     1981 agaacaaatg atgtttttgc atttagactg agaattgacg gaagcccaat tcaggctaat
     2041 gatgccgatc tgcttactaa tggaaaatat aatatattgc tggatctgga tggagacggt
     2101 ttcaaagaat tctggatact tgttgatggt aatgcagata acatgcttgt attacatgaa
     2161 gatggagctc caggtactca ggctgctgat gaagtagctg atttagtaga cacattctct
     2221 gcttgctctg atgaagcaac aattacatgt caaagcggtg gaatggatac aagccatact
     2281 cgaacctccc agttagcagg aacaatcggc gccggcagtg atacagagtg gtatatagat
     2341 attcaggttc ctgcatcctc tctttgcgct gtaggtgtca catgtgattc tgcaacacag
     2401 ttggcagatt gtgattcaaa cttgggactg tttgcaacaa ccagtgactc aaatacagac
     2461 ccgcttcaaa aagacctcat agcacaatgc cctgatacca ataatagtgg tggtactgat
     2521 cctggcgatg aatgtacatt cagccagtct acacctatta ccctttctta tttttcatct
     2581 tcagtaggtg ctagtggagc agttttgaat tggtcaactg ctacagaggt tggaaatgcc
     2641 ggatttaata tatatgccac aacagcacaa ggcttggtac aaattaatga cagtataatt
     2701 cctagcaagg taatagattc tatgtctcct gtggattatt cgtttgctgc aaatgccagc
     2761 ttgtcaagcc ccagaattca ggtccacctt tagagttaga atttgctgat attttgtcat
     2821 tgagttgaag tacatgtttg ctcatagttt ttggatttat tatctgatat aacagctcgt
     2881 ggagcag
//
LOCUS       DBNGSV010000271         4331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_950044, whole genome shotgun sequence.
ACCESSION   DBNGSV010000271 DBNGSV010000000
VERSION     DBNGSV010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_950044"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="AC159U_10010"
     CDS             <1..964
                     /locus_tag="AC159U_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3337427.1"
                     /translation="GLVGFIENLELQQKALELAKDLQQRNEIQVDVGVLPPITVTQAK
                     SEVAARQVDVITAENNVEAVSDRLKNILVLPLDSRINPVDDPNKEFIDYRDSEIIEES
                     FKNRPEIKQVKLELQKNEKLRKFYSNQRLPRLSVQGTLEYQGLGGDENPNRIDFGGGI
                     LEPISEQFNSPGDAFDQLYDGEFLTWSILGVFSYPLFNWQARGDYIKAKADLNRTVIE
                     YDKVIDDVNLDVRNSLRQIENSKRRIEAANLSVDLATQVLSNEEERLKVGIGTTREVL
                     EAQRDLVDARASQIRAIADYNIALARLEKAKGTIIQSKGVVLKD"
     gene            complement(961..2523)
                     /gene="purH"
                     /locus_tag="AC159U_10015"
     CDS             complement(961..2523)
                     /gene="purH"
                     /locus_tag="AC159U_10015"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3337428.1"
                     /translation="MQKAKRALLSVWDKKEINKFAEGLVKLGIEIISTGGTAKKLKDS
                     GIQITDISEYTNSPEMLGGRVKTLHPRIHGGILSIRDNETHIVDMEKNNIGPIDIVVV
                     NFYPFIDVIKKDGVSLDEAIENIDIGGPTMLRAAAKNYKYVSIIHDPADYKIVLKELK
                     KNGGHVSVETNYELSKKAFSYVANYDAAISNFLGSTNLDGSKIKFPDSLTMHYSKSLD
                     LRYGENPHQDGAFYIQPDFNEPSVSNSSQLQGKELSLNNIYDTDSALEAVKEFKETAC
                     VIVKHNNPCGVATGSSVKEAFLNAKECDPVSSFGGIVAFNTEVDELVATEMTEMFLEV
                     IIAPEYTEKALEILSTKKNVRVLKTPPLKNLSQSYLDLKKVTGGALFQDKDLGVSSDF
                     DNIKVATSNSPTHQQMEALKFAWIVCKHVKSNTIILAKPGQTVGIGAGQMSRVDSVKL
                     CGIKANISTDGSVLASDAFFPFRDGIDEAAKLGVKAIIQPGGSIRDQDIIDACEEHGI
                     SMVFTGVRHFKH"
     gene            2687..3748
                     /locus_tag="AC159U_10020"
     CDS             2687..3748
                     /locus_tag="AC159U_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3337429.1"
                     /translation="MKRDKSSSVKVGIFVLLSIAAFIIFVFMLGGENKLFTKSYKVQT
                     SFTNVAGLSEGASVRLSGVRIGSVKEIEFPKDIDKNFIVVLMDVKEDGVRRIGSDAIA
                     TIKTEGLLGDRYIEIIKGSKETPKEIKDVIKINSFNPADITKLIDQSDELIGNIINIS
                     ESLDEIVKAFGKEENINNISNTIASFRRSIEAIEKEDGILNTLIYGKENQTPGDKNTL
                     EKLDETIALLNDLLGEIKSGDGLLTALLFEEDLKIKVDNAITNIEKAAESIGDDKGIA
                     SDLKDTVANLKSISEKLDEGEGTIGAMINDPLLYDQLNSVLGEAERSRFVRATIKYLI
                     EQKLKREKENKTETKLYGY"
     gene            complement(3747..3820)
                     /locus_tag="AC159U_10025"
     tRNA            complement(3747..3820)
                     /locus_tag="AC159U_10025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3784..3786),aa:Gly,seq:ccc)
     gene            3886..>4331
                     /locus_tag="AC159U_10030"
     CDS             3886..>4331
                     /locus_tag="AC159U_10030"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR3337430.1"
                     /translation="MKNKRKIDKLITESVNPDTVDIDISSTSRIIKLISDEDRKISKA
                     VYEQRKLIEAAIELIYKRIKQGGRIFFVGAGTSGRLGVIEAAECPPTFGTSPNLVQAI
                     MAGGNRAVFHSVEGAEDSYKDSLEQLEKKKFSHKDVVIGIAASSSTP"
BASE COUNT         1427 a          714 c          874 g         1316 t
ORIGIN      
        1 gggacttgtt ggatttattg aaaatctgga gctgcagcaa aaagcacttg aacttgctaa
       61 agatctgcag caaagaaatg agattcaggt tgatgtaggt gttcttcctc caataactgt
      121 tactcaggct aaatctgaag tagctgcaag acaggtagat gtgattactg ctgaaaataa
      181 tgttgaagca gtttctgata gattaaaaaa tatacttgta cttcctttgg attctagaat
      241 aaatccggta gatgatccca ataaagaatt tattgattac agagatagtg aaataataga
      301 agagtcattt aaaaatagac ctgaaataaa gcaagtaaaa ctcgaattac aaaaaaatga
      361 aaagcttagg aaattttatt ccaatcagag gcttccaaga ctctcggtac agggcacttt
      421 ggagtatcag ggacttggtg gggatgaaaa tccaaataga atagattttg gtggtggaat
      481 attggaacct atttcagaac agttcaatag ccccggtgat gcattcgatc agctttatga
      541 cggagaattt ttaacatgga gcattctggg cgtttttagt tatcccctgt ttaactggca
      601 ggccaggggg gattatataa aagcaaaagc tgacttaaac agaactgtaa tagaatacga
      661 taaggtcatt gatgacgtaa atcttgatgt aagaaattca cttagacaaa ttgaaaacag
      721 taaaaggcga atcgaagcgg ctaatctatc ggttgatctt gctacacagg ttttatccaa
      781 tgaagaagaa aggcttaagg ttggtatagg aactaccaga gaagttttag aagcacagcg
      841 tgaccttgtt gatgcaagag cgagtcaaat aagggctata gcagattata atattgctct
      901 tgcaaggctt gaaaaggcca agggtacaat tattcagagt aaaggggtag ttttgaagga
      961 ttaatgctta aagtgcctta ccccagtaaa caccattgat atgccgtgct cttcacacgc
     1021 atcaataata tcctgatccc ttattgatcc accgggttgt atgatagctt ttacgccgag
     1081 tttggcagct tcatcaatac catctctaaa aggaaaaaaa gcatcagaag caagtactga
     1141 accatcagtt gatatatttg cttttatacc acaaagcttt actgaatcca ctctgctcat
     1201 ctgtcctgcg cctattccta ctgtctggcc gggttttgca agtataattg tgtttgattt
     1261 tacatgtttg catactatcc atgcaaattt tagagcttcc atttgctgat gggttggtga
     1321 atttgatgtg gcaaccttaa tattatcaaa atcagaactt accccaaggt ctttatcctg
     1381 aaatagagca ccacctgtaa ccttttttaa atcaagataa gactgcgaga gatttttaag
     1441 tggtggagtt tttaatactc taacattttt ctttgtagag agtatctcca gggctttttc
     1501 ggtatactct ggagctatta ttacctctaa gaacatctct gtcatctctg ttgctacaag
     1561 ctcatctact tcggtattaa atgcgactat tcctccaaat gaacttacgg gatcacattc
     1621 ctttgcattt aaaaaagctt cttttacact actgccggta gcaacaccac agggattgtt
     1681 gtgctttacg atcacacatg cagtctcttt aaattccttt actgcttcaa gagcagagtc
     1741 tgtatcataa atattattta gagaaagttc cttaccctga agctgggatg aattggaaac
     1801 agatggctca ttaaaatccg gttgaatata aaatgctccg tcctggtgag ggttttcacc
     1861 atatcttagg tccagtgatt ttgaataatg cattgtaaga gaatcgggga atttaatttt
     1921 agagccatcg agatttgtag agcctagaaa gttagagatt gctgcatcat agtttgctac
     1981 atacgaaaat gcttttttag acaattcgta gttagtttca acagatacat gcccgccatt
     2041 tttcttaagt tcctttaata ctattttata gtctgcagga tcgtgaatta tacttacata
     2101 tttatagttt ttggcagcgg ctcttagcat tgtcgggccg ccgatgtcta tattctctat
     2161 ggcttcatca agtgaaactc cgtctttttt tattacatct ataaatggat aaaagttaac
     2221 aactactatg tctatagggc caatattgtt tttttccata tccactatat gtgtctcatt
     2281 atctctaatt gacaaaatac ccccatgtat ccttggatgt agagtcttaa ctcttcctcc
     2341 aagcatttca ggagagtttg tatattcaga aatatctgtg atctgaattc ctgagtcctt
     2401 taatttttta gctgtaccac cggtggaaat tatttcgata ccgagtttta caagtccctc
     2461 ggcaaacttg ttaatctctt ttttatccca tacacttaaa agtgctcgtt tagctttttg
     2521 catcgtgatg tccttattgt gttaaattag atttgaatta atggtttttt tatgaataaa
     2581 gtaggtaaat gtttattgta aatcataatt aacattactt ttgtgaactt gtcaattgtt
     2641 acgtatactc taagaattca ggaaatactt tggttaaata cttctaatga aacgagataa
     2701 atcatcatct gtaaaggttg ggatttttgt acttctaagc atagctgcat ttataatatt
     2761 cgtgttcatg cttggtggag aaaataaatt atttacaaaa tcttataaag tacagacatc
     2821 gtttacaaac gttgctggcc tttcagaagg ggcttcggtt agactatcgg gggtgaggat
     2881 tggaagtgtt aaagaaattg agtttccaaa ggacatagat aaaaatttta ttgttgtact
     2941 aatggatgtc aaagaagacg gtgttagaag gattggttct gatgccatag cgactataaa
     3001 aaccgagggg ttacttggag ataggtatat tgaaataata aagggatcaa aagaaactcc
     3061 caaagagatc aaggatgtaa taaagattaa cagttttaac cctgctgata taacaaagct
     3121 gattgaccag tctgatgagc ttataggaaa tattataaat atatctgaga gtcttgatga
     3181 aattgtaaaa gcatttggta aggaagaaaa cataaataac atatcaaaca caattgcttc
     3241 attcagaagg agtattgagg caatagaaaa agaggatgga atattaaaca cgctaatata
     3301 tggaaaagaa aatcaaaccc ctggggataa gaatacactt gaaaaactcg atgaaactat
     3361 tgcactctta aatgatcttc ttggtgaaat aaaaagcgga gatggtcttc tcacagccct
     3421 tctctttgaa gaagatttaa aaataaaggt agacaatgca attactaata ttgaaaaggc
     3481 agccgagtca attggtgatg ataagggtat tgcaagtgac ctaaaagaca cggtggcaaa
     3541 tctaaaaagt atttcagaaa aactcgatga aggagagggt actattgggg ctatgataaa
     3601 cgatccgctc ctatatgatc aacttaattc cgtacttggt gaagccgaaa gaagcaggtt
     3661 tgttcgggct acaatcaaat atcttattga acaaaagtta aaaagagaaa aagagaataa
     3721 aactgaaact aagctttatg gatattagcg ggtgacggga ttcgaacccg cgaccctaag
     3781 cttgggaagc ttatgctcta gccagctgag ctacacccgc agttgctttt aaattaaagc
     3841 aaattaactg tataaagtca atcgcattac caagacttaa tcataatgaa aaacaagaga
     3901 aaaatcgaca agcttattac agaatcagta aacccggata cggttgatat agatatatca
     3961 tctacgagca ggataataaa actaatctcg gatgaagacc gtaaaatatc aaaagcggtt
     4021 tatgagcaaa gaaaattaat agaggcagcg attgagctaa tttataaaag aattaaacaa
     4081 ggtggcagga tattttttgt aggtgccggc acaagcggca ggcttggggt tattgaagca
     4141 gctgaatgtc cgcctacttt cgggacatca ccaaatcttg ttcaggctat tatggcaggt
     4201 ggtaatagag ctgtatttca ctctgtggaa ggcgcagagg actcatacaa agatagccta
     4261 gagcagcttg agaaaaagaa gtttagccat aaagatgttg taattggaat tgctgctagc
     4321 agttctactc c
//
LOCUS       DBNGSV010000272        11093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1088267, whole genome shotgun sequence.
ACCESSION   DBNGSV010000272 DBNGSV010000000
VERSION     DBNGSV010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1088267"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1446)
                     /locus_tag="AC159U_10035"
     CDS             complement(346..1446)
                     /locus_tag="AC159U_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3337431.1"
                     /translation="MKLTHLLAKKLEIPFKFNFKHALADRTKTETILVTATSSNKNVG
                     YGEGCPRTYVTNESTDTSLNFIGEHRKQISEISTLQDLQSFMDRYKKAINSNPAAWCA
                     VEIALLDLLGKEEEQSVEDLLGLSELRNEFRYTAVIGDWDINTTKTFFEKFLSMGFKD
                     FKLKLSGDLNQDSLKINVIRQSKRTDITLRVDANKLWESSEKALSYLSSLDCQFFGVE
                     EPLEKFSYTDLMELSKKLGAKIILDESFKKIGDFDKLENRTDVFIINIRISKMGGILR
                     SLGIAKIAAAKGFGIILGAQVGETSILTRAALPVASTYSNNVIAQEGAFGEFLLERDL
                     CTPSIKFGAGGILQISDYLEPNSYGLGLNIQG"
     gene            complement(1443..3092)
                     /locus_tag="AC159U_10040"
     CDS             complement(1443..3092)
                     /locus_tag="AC159U_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HIR3337432.1"
                     /translation="MINTLIKLYFFIIRLFVWRPFIKKTSDPQTEQNAALRSLLEKNS
                     NTVFGKKYGFSAINNYEDFKRSVPVHTYEMLREYIEKQIQAREPYLTESSPIMYAQTS
                     GTTDKPKLIPILEKTVKNYKMTQCISNYSQYREEPSMFSGKILAIVSPATEGKLENDI
                     PYGSLSGLLYKNIPRIIRSKYVLPYEIFDLENYDEKYFLISVLSVAQKNISTLAAANP
                     STILKILEIINKRFNEITDIIESGKINSNINISKQANHILSNHLNQNKTRADELRNII
                     RSNNSIYLKDIWPDLKAVLTWTQGNCSLLIPKLRKILPNNTKIFELGYLCSEFRGSVV
                     IDANSDSGIPIIDENFYEFIERDDWDLGKNEYRTISEIEIGKQYYIIVTTAHGLYRYF
                     INDIVEVTGKFNNTPTIKFAQKGRGVTNITGEKLYEEQVIKALCNVKDKACIEFNFFT
                     LLANPIDLSYELFVESEKIPVADLSPIIDEALKMINLEYKAKRDSGRLKAIKLNYIKN
                     GTSENYKNYCIQNGQRDSQFKFTHLMYKKECSFNFSNHIIK"
     gene            complement(3100..3966)
                     /locus_tag="AC159U_10045"
     CDS             complement(3100..3966)
                     /locus_tag="AC159U_10045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3337433.1"
                     /translation="MPRLKNLTSCIAYPAVIVISFVIHIVFIGLGLSIFISTYTPVII
                     AALIMCGLEFFIPHRDSWNASSKDIKNDLLYMGLVQVLLPKFLSFFFAITLIAYLQST
                     NLQILELWPDHLPIFVQAAIMLLGADFLRYWLHIASHRWDLLWKLHSVHHSPSKLYWI
                     NVARFHPIEKMLQFLLDALPFIILGVGEQVVSLYFIFYAVNGFFQHSNIELRLGFLNY
                     IVSGPELHRWHHSVVIRESNQNYGNNLIIWDLLFGTWFLPKDKEVSELGLQDKNFPTD
                     FINQIKAPFTKK"
     gene            4683..5264
                     /locus_tag="AC159U_10050"
     CDS             4683..5264
                     /locus_tag="AC159U_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3337434.1"
                     /translation="MGVNKIPKQTTKLEPIENTAIKLFAKKGIKEVTIKDIASEAGCS
                     DGALYRHYKSKEEMAWTLYKREVEGFGERIKEMLGDKALPNEKKIYNAVELFYDFYDK
                     NPTKFSFILLSQYNFPRQLKLNKKLNPYYIMQECLQGMTKGKKIKDLDLLSGMIFGLI
                     LEPARMKATNELKGKLKDRTGTVYKACMSIINI"
     gene            complement(5279..6238)
                     /locus_tag="AC159U_10055"
     CDS             complement(5279..6238)
                     /locus_tag="AC159U_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337435.1"
                     /translation="MPQNSRQVFRTHSNYLNAIRYIASGGIEDNNQKSLELLAEKVFE
                     SGCLKLEKKTEFNTNDIHHSLNNAWGVEALYKMGNLFIKEDDLIRLSNNWCIVQTYYA
                     LYYSTQALAITEGYPKPRTHAQVQKIFYVMWSEISWLITPWCISFDENGFHNLAPGIY
                     PDDHIHSWKKVEDESALSLFCKALRTTREEYISEALAKRRDRLAKKKDTSTVKLTDEQ
                     KMQTITRVRPTTLMSYLFRLKRKTNYEQSSILAIGPQNEFQSQELRNNLSFIIESTLL
                     ITEFIISRLIGEDIYYNWCREWEKYKAPAFDHIGPEERFEIVF"
     gene            complement(6327..6629)
                     /locus_tag="AC159U_10060"
     CDS             complement(6327..6629)
                     /locus_tag="AC159U_10060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3337436.1"
                     /translation="MIVLIAEFKTKPDCRDKMIEISEPMVKLSNQEKGCISYEFMQNP
                     YEPDSFKFVERWRSQPDLDEHFEKSYFKNFTKKFPELLQEPENLNIYHVSEDNMVA"
     gene            6714..7100
                     /locus_tag="AC159U_10065"
     CDS             6714..7100
                     /locus_tag="AC159U_10065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3337437.1"
                     /translation="MSSEKYQKGWDKLNEISPGAGDRTVASLSDIAPDMVNYLMEFVF
                     GEISSRPGLDMRAREITAVAALTALGSYPTQLKVHIKGALNCGCTREEITEVIMQTLV
                     YAGFPAALTGLRLAKECFAEIDEKNK"
     gene            7145..7480
                     /locus_tag="AC159U_10070"
     CDS             7145..7480
                     /locus_tag="AC159U_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337438.1"
                     /translation="MKLRTEVEKEFNIKKGDTHHPIEFRLVNMHNLLPIDISSAQSVF
                     ILLKDGQEVLRSDVNLEHGIAGIGRHDWKEGETDREGVYDGYFELTCKDGNYFRIPEG
                     QEFIRIEIA"
     gene            complement(7601..8365)
                     /locus_tag="AC159U_10075"
     CDS             complement(7601..8365)
                     /locus_tag="AC159U_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3337439.1"
                     /translation="MKNIIILSLLVLVTAVLSTGCRNNKNAQADDAMGNQVPISVASI
                     IIETNATDGDSGIQIFVDGEGWDLLDIMAPDGTTLVDIKGESSVGLQGINELFFESAE
                     PSFDELPLDQFLARFPEGEYKIMGITTEGDELAGTATLTHTLPCGPEIKTPEEDEEVD
                     PDDTVIGWEEVTQEFDPDGECKDSAELEIVRYEVIVIREDEEPELEFVVTLPPDVTEI
                     TVPSEFLLPDTEYKFEIVAMEASGNQTLVESSFSTP"
     gene            8720..8848
                     /locus_tag="AC159U_10080"
     CDS             8720..8848
                     /locus_tag="AC159U_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337440.1"
                     /translation="MVKKLQRRNKLFSETENNRKNRDTYANTNSENGFKEKGHIEL"
     gene            complement(8914..9447)
                     /locus_tag="AC159U_10085"
     CDS             complement(8914..9447)
                     /locus_tag="AC159U_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337441.1"
                     /translation="MGIIFKYFILLILFLQYSHPVLSQTARDYYLKGVNLLNSGEYES
                     SINEFNSALQLDPLHADSFYHRGIAKAHLHDFEAASVDMNKAIELNQEMKEPYSERLP
                     DYTNKEELRQAIRDSTKSIENNPANGKAYLIRGVAKIKLGNESSGCMDIYNANKFGYT
                     VAYSFIKDFCDSFGLQN"
     gene            complement(9806..>11093)
                     /locus_tag="AC159U_10090"
     CDS             complement(9806..>11093)
                     /locus_tag="AC159U_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337442.1"
                     /translation="LGRGRYVGPDLYKIDQRYTKQEIINWMVNSQQIYQQKGKAPLNE
                     GYPPMPPMNVPADQAEKIYDYLVKFQIFNDRQEEGSIKGVIVNKTLNQNIAGQDIKLT
                     SYVGDKSREQYSVKSDSKGQYLFENLGWDRSYVVSLMFNGVEYATGKLVFTPEESVKT
                     LDLPVFDVSTDGSLISLDSSHVIIEIQGETASFAEMFVFNNGSNSVFVGKDIDSEQKE
                     TLSFDIPAGANTLQFHHGMSEETSILKDGKLISTQAVQPGLTRIIFSYQIPIDRHTEI
                     NRKSNYLINSQLIMVSDNGYEMDIQGLQRGEQVDMHNKKFIKWSGNNLQKGHDIKISI
                     SKPIVVTDYAKWIVLIIVVLLIAGSIGYSFLRKNKTQTQTSSDDESLESLKDSLIRDI
                     ARLDDMFEANDIGQQEYQKLRDEKKKKLLEIVETSK"
BASE COUNT         3329 a         1982 c         2034 g         3748 t
ORIGIN      
        1 ctgcaagagt aaaaacaata gcaagtaata ttcctcctgt aaaccagtta agctgtcctt
       61 gcattataaa cctccagcat tttatatttt gcaatctttt tacagcacta taagtgcaat
      121 ggtcgtacca attagaacgt aatgattaat atctgtttag gtaatttatt ttatatattc
      181 atagaaaggt tctaatatgc tgatatcatt gaaaaataat tggaattaag agactgcaat
      241 cctggtttgg gttgaaaata ttactattat atttcgaaca tttcgttgtt tgctattatg
      301 gaaaaagtga aagatgtcag aaaatctgat acaaattata tttatttagc cttgtatgtt
      361 taaacccaga ccgtacgaat tgggttctaa ataatctgag atttgaagaa tgccgcctgc
      421 tccaaattta atcgaaggag tgcataaatc cctttctaat aagaattcac caaatgctcc
      481 ttcctgagct attacattat tggaatatgt gcttgcaaca ggtaatgcag ctcgtgtcag
      541 aatacttgtt tctcctacct gagcgcccaa aataattcca aaacctttcg cggcagctat
      601 tttagcaata ccaagcgagc gtagaattcc acccattttt gatatacgta tatttataat
      661 gaatacatct gttctgttct ctaatttgtc gaaatcccca atttttttaa aagactcatc
      721 cagaatgatc tttgctccca gtttcttaga taattccatc aaatcggtat aagaaaattt
      781 ctctaaaggt tcttccactc caaaaaactg acaatccagg ctactaagat aagataatgc
      841 tttttcagaa ctttcccaaa gtttattggc atccactctt aatgtaatat ctgttctctt
      901 agactgcctt atcacattaa tttttaatga atcctgattc aggtctcctg atagctttaa
      961 cttaaaatct ttaaaaccca tagataagaa tttttcaaag aaagttttag ttgtatttat
     1021 atcccaatcg ccgattactg ccgtatatct aaattcgttt ctaagttctg aaagacctag
     1081 caaatcttct acagactgtt cttcttcttt tccaagtaga tccagaagtg caatttccac
     1141 agcacaccat gccgcgggat ttgagttaat tgcttttttg tatctgtcca taaaggattg
     1201 taaatcctgt agagtgctga tttctgaaat ctgctttcta tgttcaccga taaagtttaa
     1261 tgaagtatct gtggattcgt ttgtcacata ggttcgagga catccttctc catatccaac
     1321 atttttatta gatgatgttg ccgttaccaa tatagtctct gtttttgtcc tatcggccag
     1381 ggcgtgtttg aaattaaact taaatggaat ttcgagcttt tttgcaagaa ggtgtgtaag
     1441 cttcatttta ttatatgatt gctaaaatta aaactgcact cctttttata cataagatga
     1501 gtaaatttaa attgtgagtc tctttggccg ttttgtatgc agtagttttt atagttctca
     1561 ctggttccgt ttttaatata atttaattta atagccttta atctaccgct atctctcttg
     1621 gctttatact ccaaatttat catttttagt gcttcatcga taattgggga taaatctgcc
     1681 acgggaattt tttcagattc tacaaacagc tcataagata ggtctatagg gtttgccagc
     1741 aatgtaaaga agttaaactc aatacaggct ttatctttta cattacaaag agcctttatt
     1801 acctgttctt catagagttt ctcccctgtg atgtttgtaa ctcctcttcc tttttgggca
     1861 aacttgatcg tgggcgtgtt gttgaatttc ccggtaactt ctactatgtc gtttatgaaa
     1921 tatctataaa gcccatgggc tgtggtgaca ataatgtagt actgtttgcc gatttcgatt
     1981 tcagaaatgg ttctatattc attctttcca agatcccagt cgtctctttc aatgaactca
     2041 taaaaattct catcgattat tggtatgccg gagtcgctgt ttgcatcaat aactacagat
     2101 cccctaaatt cactacaaag atatcctaac tcaaatatct ttgtattatt tggcagtatt
     2161 ttccttagct taggaattag cagtgagcaa ttcccctgag tccaggttaa tactgctttt
     2221 aaatccggcc atatatcttt gaggtatatt gaattgttgc ttcttatgat atttctcagt
     2281 tcatctgctc ttgttttgtt ttgatttagg tgattgctga gtatatgatt tgcctgtttt
     2341 gatatattta tatttgaatt aatttttcca ctttcaatga tatctgttat ttcattaaac
     2401 cttttgttaa tgatctctaa aatcttgagt attgtcgaag gattggcggc tgcaagagtg
     2461 ctaatgtttt tttgagcaac gcttagaaca gatattaaaa aatatttctc atcatagttc
     2521 tctaaatcaa agatttcata aggaagtaca tattttgatc ttataattct tggaatgttt
     2581 ttataaagaa gaccggacag cgatccgtag gggatatcat tttctaattt accctcagtc
     2641 gccgggctta ctattgcaag aattttcccg gaaaacatcg aaggttcttc cctgtattga
     2701 gaataatttg aaatacactg tgtcattttg tagtttttta cagttttttc caatatgggt
     2761 atgagttttg gcttgtctgt cgtaccactt gtctgtgcat acataatggg ggaggactca
     2821 gttaagtatg gctccctggc ttggatttgt ttttcaatat attctcttag catttcgtag
     2881 gtatgaaccg gcactgatct tttaaaatct tcataattgt ttatcgcaga aaatccgtat
     2941 ttcttgccaa agaccgtatt actatttttc tctaaaagag atcttagagc cgcattttgc
     3001 tcagtctgcg ggtcggaggt tttctttata aaaggtctcc atacaaagag cctaatgatg
     3061 aagaaatata gtttgattaa tgtgttaatc attttcgcct cattttttag taaatggcgc
     3121 ttttatctgg tttataaaat cggttgggaa gtttttatct tgcagtccca attcagatac
     3181 ttctttgtct tttggcaaaa accatgtacc gaagagcagg tcccatatta tcaggttgtt
     3241 gccgtagttt tggtttgatt ctcttattac tacagagtgg tgccagcggt gaagctccgg
     3301 gccgcttacg atgtaattta gaaatcccag ccgcagttca atattggaat gctgaaagaa
     3361 gccgtttact gcgtaaaaaa tgaagtagag tgaaacaact tgctcaccca cacctaagat
     3421 aataaagggc agggcatcga gaagaaactg aagcattttt tcaattggat ggaaacgggc
     3481 aacgtttatc cagtacagtt tggatggtga gtggtgaaca gaatggagtt tccatagaag
     3541 gtcccatctg tggcttgcaa tatgaagcca gtacctaaga aagtcagctc cgagtagcat
     3601 aattgcagcc tggacgaaaa taggcaggtg gtctggccaa agctccagta tctgcaagtt
     3661 ggtcgactgt aaatatgcaa taagtgttat agcaaagaag aaagaaagaa actttggaag
     3721 taatacctgc acaagtccca tgtatagaag gtcattcttt atgtcttttg aacttgcatt
     3781 ccagctatcc ctatgcggga taaaaaattc aaggccacac ataataagtg cggcaattat
     3841 gaccggcgtg tatgttgaga tgaaaatact taacccaagc cctataaaaa ctatatgtat
     3901 tacaaatgat atcactatta cagctggata agctatgcaa gaggttaaat tctttaatct
     3961 aggcattatt taatccacct cctttgttta ataaatgaaa atatccaaac aatactaaga
     4021 agcatgcgag caccaggtga aaaaatgatg ggataggata tgcaaaccat tttacatctt
     4081 gtagtagagt taatcccgca ataagtctaa agaacataat agcaaaataa agagtgccga
     4141 gagtaatgaa tatatatcca gtttttatac ttagaacaat tttgtctaaa gcaattttgt
     4201 aaatagtttt agatagaaca aagattgtta atatctgagc tgtaaataga aatttgtaag
     4261 atagtatacc gctgtcccag gattcaaacg gaggcagaaa tgtaagatct aagaaatatt
     4321 gcagtagttg tgcgacaact ctgaatataa agagtcctag aagtaagata aagcagagga
     4381 ttaatttctt tgttttcttt gtcttaatat tcaacataac gcattcctca aaaatgtcct
     4441 ttcattacta tcgtttgtat gtgaatgaac atttattcac atattataat aaaataattg
     4501 ctgtgtcaag agattttata ggccaggttt tatagttgct gtatagttgg ctgtgcttga
     4561 tttgaattaa gatggttagt atatggttgt tttgtttatt tggaataatt gagatgaatc
     4621 atttgagtaa tttataaata attatgtatt cacttatttt tctattatat ttactctaaa
     4681 acttgggagt aaataagatt ccaaaacaaa ctactaaatt agagccgata gaaaacactg
     4741 ctataaagct ttttgcaaag aaggggataa aggaagtaac aataaaagac atcgcctctg
     4801 aggctggctg ctcggatggt gcgctttacc gccattataa gagcaaagaa gagatggcgt
     4861 ggactctgta taaaagagaa gtagaaggtt ttggggagcg tataaaagag atgttgggtg
     4921 ataaagcgct tccgaatgag aagaaaatat acaatgctgt tgaactgttt tatgattttt
     4981 atgataaaaa cccgactaaa ttcagcttta ttctgctatc tcaatataac ttcccccggc
     5041 agttgaagct taataaaaaa ctaaacccgt attatattat gcaggaatgt ttacaaggca
     5101 tgacgaaagg aaagaagatt aaagatttag acctcctaag cggtatgatt tttggtctta
     5161 ttctagaacc tgccagaatg aaagctacaa atgagcttaa gggtaaattg aaagacagaa
     5221 ccggaacagt ttataaggcg tgtatgagta ttattaatat ataatcgtat atatatactt
     5281 agaaaacaat ttcaaacctc tcctcggggc ctatatgatc gaaagccggg gctttgtatt
     5341 tttcccactc tctgcaccaa ttgtaatata tatcttctcc tataagtctt gagatgataa
     5401 actctgttat cagtagtgtt gattcgatta taaatgaaag gttatttcgt aattcttgtg
     5461 actggaattc attttgcggg ccaatggcta ggatgcttga ttgctcgtaa ttggtttttc
     5521 tcttcagcct gaataaataa ctcataagag tagtagggcg aactctcgta atagtctgca
     5581 ttttctgttc atcggtaagt tttacggtgg aagtatcttt tttctttgcg agtctgtctc
     5641 tcctttttgc aagagcttct gaaatatatt cttctctggt tgttctaagc gctttacaaa
     5701 aaaggcttag tgcagattca tcctcaacct ttttccatga atgaatatgg tcatccggat
     5761 atattccggg agctaggttg tgaaacccat tttcatcgaa actgatacac cagggggtaa
     5821 ttagccagga tatttctgac cacatgacat aaaatatttt ctgaacctgg gcatgggttc
     5881 ggggttttgg ataaccctcg gtgattgcta gtgcctgagt ggaataatac agcgcgtaat
     5941 atgtttgtac tatacaccaa ttgttagaga gcctgatgag gtcatcttct tttataaaaa
     6001 gattacccat tttgtatagg gcctccactc cccaggcatt gttaagagag tggtgaatat
     6061 cattagtatt aaactctgtc tttttttcta gttttagaca gcctgattca aatacctttt
     6121 cagccagcag ttctaaagat ttttgattat tatcttctat gccgccagat gcgatatatc
     6181 ttatagcgtt taaataattt gagtgagtcc tgaaaacctg ccgtgagttt tgtggcatac
     6241 ataataagta taattgattg tgattatgtt tataaggtat ttatttgtta aatctgattt
     6301 ttatatatgt aaaaataata acaatcttat gccaccatat tatcttcgct tacatgatat
     6361 atgtttagat tctcaggttc ttgaagcagt tccgggaact tttttgtaaa gttcttgaaa
     6421 taagattttt caaaatgctc gtcaaggtcg ggctggcttc gccacctttc aacaaattta
     6481 aagctatcag gctcgtaagg gttttgcata aattcgtagc ttatacagcc tttttcctga
     6541 ttagagagct ttaccatggg ctctgagatt tctatcattt tatctctgca atcaggtttt
     6601 gttttaaatt ctgctattaa aacgatcatc ttttattccc cctacttcat ttagtttatc
     6661 ttttttaatt attatataac atatatcata atatttttac aaaggagtag aaaatgagtt
     6721 cagaaaaata tcagaaagga tgggacaagc taaatgaaat ttcaccaggt gcgggagaca
     6781 gaacggttgc aagcctgagc gatatagcgc cagacatggt gaactatctt atggagtttg
     6841 tgtttggaga gatttcatca agacccgggc ttgatatgag ggcaagggaa ataacagcag
     6901 ttgctgcgct tacagcactt ggctcatatc ccacacagct taaggttcat atcaaaggag
     6961 cacttaactg cggctgtaca agagaagaga ttaccgaagt aatcatgcag acccttgttt
     7021 atgccggatt tccggcagcg cttacgggac ttaggcttgc caaggaatgt tttgctgaaa
     7081 ttgatgaaaa aaataagtaa aattaagtta aattcagtta ataatttgat acaatattaa
     7141 ttagatgaaa cttaggactg aagttgaaaa agagtttaat atcaaaaaag gtgatacaca
     7201 ccaccctata gagtttaggc tggtaaacat gcacaatttg ctaccaatcg atataagcag
     7261 cgcacaatca gtttttatat tactcaaaga tggccaggag gttttaaggt cggatgtaaa
     7321 cctagagcat ggaattgccg gtataggaag acatgactgg aaagagggtg aaactgaccg
     7381 tgagggagta tatgacggtt acttcgaact tacgtgtaaa gacggcaatt attttagaat
     7441 tcccgaagga caagaattta ttagaataga aatagcttag tgaaagcaag aaaagattgg
     7501 gggcattaat taacttgata tagacggaca gacactgtaa ttgggatatt acactgtctg
     7561 tcctaggcat atatagaaag gtcttataaa aatctagatt ttagggtgta gaaaaactgc
     7621 tttctacaag tgtctgattg ccgcttgctt ccatggccac tatctcaaat ttatattcgg
     7681 tatccggaag caaaaattct gatggcacag taatctcagt tacatccggc ggtagtgtga
     7741 ctacaaactc aagctcaggt tcttcatctt ctcttatcac tattacctca tatcttacga
     7801 tctcaagctc ggcggagtct ttgcattctc catctggatc aaactcttgt gttacctctt
     7861 cccatccaat aaccgtatca tccgggtcta cttcttcatc ttcttcaggc gtttttattt
     7921 caggtccgca gggcagggtg tgagtcagtg tggcggttcc agcaagctca tctccttcgg
     7981 tggtaattcc catgatttta tattcaccct cagggaagcg tgctaaaaac tgatctaagg
     8041 gaagctcgtc aaatgaaggc tctgcgcttt caaaaaatag ttcatttatt ccctgtagtc
     8101 ccacactgct ctctccctta atgtctacaa gggttgttcc atccggggcc ataatgtcga
     8161 gcaagtccca gccttcaccg tcgacaaata tctgaatccc agagtcgccg tcagttgcat
     8221 ttgtttctat aatgatagat gctaccgata taggcacctg gtttcccatg gcgtcatctg
     8281 cctgtgcatt tttattgttt ctgcatcctg tactgagtac agctgttact aaaaccagta
     8341 atgaaagtat gataatattt ttcatccggc tttctccttt aataaatttt aaatatagga
     8401 taaaccaccc ggattactta aaaattactg taacattaaa tttttgtaat agttatatgg
     8461 ggggattcga ataactttgt tatctatatg cctttgtaat taatacatcc ccgactggat
     8521 tcaaaccagt ggcctcagga ttaggaatac taaaagagta aatcatgtaa gtatctgaaa
     8581 tcattagata atctcgcaac tcactggaat cacaacacta attttctgtg caataagttg
     8641 catcctgtgc aaccagtgca acaattttca tctcttggtt agaaggtggt tagaggattt
     8701 ttttctgaaa atgggaggaa tggtgaaaaa acttcagaga agaaataaat tattctcgga
     8761 aaccgaaaac aaccgaaaga atcgcgacac ctacgctaat acaaattccg aaaatggatt
     8821 taaggaaaag ggacatatag aattataaaa aaccaataat tactgagcta gtaaaagcga
     8881 ccgtttttgc tctgcccttt tactcaagtc tgtttaattt tgtaatccga agctgtcaca
     8941 aaaatctttt atgaaactgt atgctactgt atatccaaac ttgttggcat tataaatatc
     9001 catacacccg ctggattcat ttcccaactt gatcttggca actcctctga taaggtacgc
     9061 cttgccgttt gcaggattat tttctatgga tttggttgaa tctcttattg cttgtctgag
     9121 ctcttcttta ttcgtataat caggtaatct ctcagagtac ggttccttca tctcttgatt
     9181 aagctcgatg gctttgttca tatcaaccga tgcagcctcg aagtcatgta aatgtgcttt
     9241 tgctataccc ctgtgataaa atgaatcggc atgtaggggg tctaactgta gagcactgtt
     9301 aaactcattg atagacgact catattcacc cgaatttagt agatttacac cctttaaata
     9361 ataatccctg gccgtttggg ataaaacagg atggctgtac tgtaagaaaa gtattaataa
     9421 tataaaatac ttaaatatga tccccataac ctattataac ttttttagga atttttatat
     9481 agatataata tcacattttc tgtgataatt ttataaattt ctgcctttaa tgagcataag
     9541 ttcgttggaa gttctggtaa tatatataaa taaacactgc aatgacagta ggttacacgt
     9601 gatagattat agtttgcatt acaaaacacg gataatatat gagacatgtg caaagcatgc
     9661 tgattgtatt tgagactaca aatttaaagt tgtagtatga ttataattaa gtagtctaat
     9721 tattcatcta ttaattgaat tattcaataa acaacacaaa gcgaccgcta tgttatccat
     9781 aaatattcag attaaacgct gcatattatt tagacgtttc tactatttct agaagctttt
     9841 tctttttctc gtcccgcagt ttttgatatt cctgctgccc tatgtcgttt gcctcaaaca
     9901 tgtcatcaag cctagctata tctcttataa gcgaatcttt taaactctcc aggctttcat
     9961 catcactact ggtctgagtt tgcgttttat tttttctgag aaatgaatat ccgatactgc
    10021 ctgcaattag taaaactacg ataatcagca caatccattt tgcatagtca gtcactacta
    10081 ttggcttgct aatactaatc ttgatgtcat ggcctttttg cagattattc ccactccact
    10141 ttataaactt cttgttatgc atatcgacct gctcaccgcg ctgcagcccc tgtatatcca
    10201 tctcgtatcc attgtccgaa accattatga gctgagaatt gataagatag tttgattttc
    10261 tgttaatttc tgtatgtctg tcaatcggta tttggtatga aaatataatt cttgtaagcc
    10321 cgggttgaac agcttgggtg cttattaact ttccatcttt taaaatagat gtttcttcgg
    10381 acatgccgtg atgaaactgc aaagtgtttg caccagcggg aatatcaaaa cttagggttt
    10441 ctttttgctc tgagtctatg tctttgccaa cgaacacaga atttgaacca ttgttgaaaa
    10501 caaacatctc tgcaaaagat gcagtttccc cttgtatttc tatgataaca tgggaggaat
    10561 ccagcgatat tagactgccg tctgtagata catcaaaaac cggcagatct aatgttttaa
    10621 cactttcttc gggagtgaat acaagcttgc ctgtcgcata ttcaacccca ttaaacatta
    10681 gtgaaactac atacgatcta tcccatccga ggttttcgaa taggtactga cccttggaat
    10741 cagatttgac agaatattgc tccctcgatt tatcaccaac atatgaggtt agtttaatat
    10801 cctgtccagc aatgttttga ttcaaagtct tgttgacaat tactccttta attgaacctt
    10861 cttcttgcct gtcattgaaa atttgaaatt taactaaata atcatatatt ttttctgcct
    10921 gatcggccgg gacattcatt ggaggcatag gagggtaacc ttcatttaat ggtgcttttc
    10981 ccttttgttg gtaaatctgc tgtgagttaa ccatccagtt gattatttct tgttttgtgt
    11041 aacgctgatc tatcttataa agatcgggtc ccacgtacct gccgcgccct aat
//
LOCUS       DBNGSV010000273         3952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1364522, whole genome shotgun sequence.
ACCESSION   DBNGSV010000273 DBNGSV010000000
VERSION     DBNGSV010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1364522"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="hisD"
                     /locus_tag="AC159U_10095"
     CDS             <1..1281
                     /gene="hisD"
                     /locus_tag="AC159U_10095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3337443.1"
                     /translation="QKLNTKNYKDKISKLRADNSHNDPKLEESVRKIVEDVRTNGDKA
                     LFKYTKKFDKFSLNAKNIKVSKKEITDAEKRLSNRIKSDLRKAAARVKSYHNTKKPEK
                     YIYKDSLGNELGWLITPIDRVGIYVPGGKAAYPSTVLMTAIAARVAGVKEIVLVTPCP
                     KGHLNDEVLYAAKISGVDAIYKVGGAQAVAALAYGTDIVPKVDKIVGPGNIFVAIAKK
                     LVFGVVDIDMIAGPSEVLIIADGSCSPDWVAADLLAQAEHDEMAVPCCVTTSAKYARE
                     VKASVLDQLSRLSRKDIAGESVKNYGRIFVVNNLDFAVEVSNNFAPEHLELCVQKPKQ
                     LLSKITNAGAIFVGATSTEAFGDYVAGPSHVLPTGGAARFSSPLSVYDYVKMPSVISI
                     SKNGFNKLSKSVMNLAYSEGLEAHALSVKTRLEK"
     gene            1306..2682
                     /gene="hisC"
                     /locus_tag="AC159U_10100"
     CDS             1306..2682
                     /gene="hisC"
                     /locus_tag="AC159U_10100"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3337444.1"
                     /translation="MDIKCSFCEESFKGHYRIVTRGSYAICIDCVSIVKEIFIEFETS
                     ETYEGGGVCSICGKHHSDFNKVFKGNKVHICNACLDEIEDELGEMKMQEKGEISVTET
                     NKRGDLSSRVRSDIAELKAYSVPHYKCSVKLDGNESPFDLPSDIREKVQDKLSTVLLN
                     RYPDPGADVLRAKLGNMSGVQPDQIVLGNGSDELIEMIIRAFAGGSGKVLIPVPTFSM
                     YKLSAVTLGCEVIGAELDRNFDLDLDLFRKHIEQDDPDVIFLASPNNPTGNCFSREKI
                     IQIIESATGVVVVDEAYCDFSGVTFLDQLSDYPNLIILRTMSKIGFASLRLGMLYASA
                     EVTKTINKIRLPYNINSLSQAVAETVLENNEALRSNISTIIAERRRVFERMKIIPGIE
                     LYASDANFILFKINNADRIYKDLIEKDILIRNFNSPGRLENCMRVTIGTTEENDAFLK
                     ALTEILSS"
     gene            complement(2684..3169)
                     /locus_tag="AC159U_10105"
     CDS             complement(2684..3169)
                     /locus_tag="AC159U_10105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HIR3337445.1"
                     /translation="MSDNILFFLTLYSAIGCGLMAGLFFIFSNTVMSALGSIKPAQGI
                     AAMQSINRVILNPIFFIVFMGTAITCLIIAVSLIWKSDQPANFYLLSGNLFYLLGSML
                     VTIVKNVPMNNALDKVDPESDEGANLWTDYLTNWTRWNHVRTVACLLACLLLIIALTK
                     M"
     gene            complement(3391..3801)
                     /locus_tag="AC159U_10110"
     CDS             complement(3391..3801)
                     /locus_tag="AC159U_10110"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR3337446.1"
                     /translation="MLVWKDDYCRGLENIDNHHKSIFRQINKLSEKVEKGEGLATIGV
                     FIKSMSSYVKLHFSIEEACMKKYKCPFADKNKEAHTKFLNAFDGFQQRLKTEGPSESL
                     VMEVHDVAENWLTNHIIRIDTNLRDSVEKQEGIQ"
BASE COUNT         1274 a          715 c          816 g         1147 t
ORIGIN      
        1 caaaaactaa acacaaaaaa ttataaagac aaaatatcga agctaagggc ggataattcg
       61 cataatgatc cgaagcttga ggaatcggtt cgtaaaattg tcgaagacgt aagaacaaac
      121 ggggataagg ctctttttaa atacacaaaa aagtttgata agttttcgct taatgcaaag
      181 aatattaagg tttctaaaaa agagataact gatgctgaaa agcgtttgtc taataggatt
      241 aaatccgatc tgagaaaagc tgctgcaaga gtaaagtcat atcacaacac caaaaaacct
      301 gaaaaataca tatataaaga cagtcttgga aatgagcttg gctggctcat aacgcccata
      361 gaccgcgtgg ggatttatgt gccgggtgga aaagcggcat atccttccac agtgctcatg
      421 actgcaatag ccgcgagggt ggccggagtt aaggagatag tgctcgtaac accatgcccg
      481 aaagggcatc ttaatgatga ggttctttat gcggccaaaa tttcaggcgt tgatgctatt
      541 tataaagtcg gcggcgcgca ggcagtggcg gcacttgcct atggtacgga cattgttccc
      601 aaagtggata agattgtagg tcccggaaat atttttgtgg caatcgcaaa gaaacttgta
      661 ttcggcgttg tcgacataga catgattgcc ggcccgagcg aagttcttat tattgctgac
      721 ggaagctgct cacctgattg ggtggcggcg gacctccttg cccaggcaga gcatgacgag
      781 atggcggttc cttgctgtgt taccacttct gcaaaatatg ccagggaagt taaggcatcg
      841 gttctggatc agcttagcag gctttcacgt aaagatattg caggagaatc tgtaaaaaat
      901 tacggcagaa tatttgtggt taataatctc gattttgcag ttgaagtctc aaacaatttt
      961 gcacctgagc acctggaact ctgcgtacaa aaaccaaaac agcttctttc gaaaatcaca
     1021 aacgctggcg cgatatttgt tggagctact tcaaccgagg cctttgggga ttatgttgca
     1081 ggcccaagcc atgtgcttcc cacaggaggg gccgcaaggt tttcttcgcc gctcagcgtg
     1141 tatgattatg ttaaaatgcc aagtgtgata tcaatttcta aaaatggatt taataaactt
     1201 agcaagtcag tcatgaacct tgcatacagt gaaggccttg aggctcatgc gctctcggtt
     1261 aagacacgat tagaaaaata atttctaatt gattggagaa gtaatttgga catcaaatgt
     1321 tcattttgtg aagagtcatt taaaggacat taccgaattg taacgagggg aagctatgca
     1381 atctgtatcg attgcgtatc aattgtaaaa gagattttta tagaatttga gacaagcgag
     1441 acatacgaag gcggcggagt ttgttcaatt tgcggcaaac atcactcaga tttcaataaa
     1501 gtttttaaag gcaataaagt acatatatgt aatgcttgtc tggatgaaat agaagatgag
     1561 ctgggcgaaa tgaaaatgca ggaaaaagga gagatatcag tgactgaaac taataagaga
     1621 ggcgatctgt catcaagggt taggtctgat attgcagagc taaaggcgta ttctgttcct
     1681 cactataaat gcagtgtgaa gcttgatgga aacgaaagcc catttgatct gccgtctgat
     1741 attagagaaa aagtacagga taaactaagc acagttctgc ttaacagata cccagaccct
     1801 ggcgccgatg ttcttagggc aaagcttggt aatatgtccg gcgtacagcc agatcaaata
     1861 gttcttggca acggctctga tgaattgatt gaaatgatta tccgtgcttt tgccggaggc
     1921 tcaggcaaag ttttaattcc cgttcctaca ttttctatgt ataagctttc tgctgtaaca
     1981 cttggttgtg aggttatagg ggctgagctt gatcgcaatt ttgatctgga tttggatttg
     2041 tttagaaaac atattgagca ggatgatccg gatgtaatat ttctagcatc tccaaataat
     2101 cccacgggca actgcttttc aagagaaaag attatccaga taattgaatc agcaaccggc
     2161 gtagtagttg tggatgaagc atactgtgat ttttcagggg ttacttttct agatcagctc
     2221 tctgactatc ccaatcttat aattttaaga acaatgtcca aaatcggatt tgcttcactg
     2281 aggcttggga tgttgtatgc ttcggctgaa gtaactaaaa caataaacaa gattcgcctg
     2341 ccttataata ttaattctct aagccaggcc gttgcagaaa cggtgcttga aaataatgag
     2401 gcattgaggt cgaatatctc tactattatt gctgagagaa gaagggtgtt tgagcgaatg
     2461 aagatcatcc ctggcataga actttatgca tccgatgcaa attttatact ttttaaaatc
     2521 aacaacgccg acagaatcta caaagaccta atagaaaaag atatacttat aagaaatttc
     2581 aactccccgg gaaggcttga aaactgtatg agagttacta ttggtacaac agaagaaaac
     2641 gatgcatttt taaaggctct taccgaaata ttgtcttcct gactcacatt ttagttagag
     2701 caataattaa taataaacag gccaataaac aagcaaccgt ccgtacatgg ttccatcttg
     2761 tccagttagt aagataatct gtccaaagat tagcaccttc atcactttct ggatcaacct
     2821 tatccaaagc attattcatt ggtacattct ttacaattgt tacaagcata gaaccaagaa
     2881 gataaaacag gtttccagaa agtaggtaaa aatttgctgg ctggtcagat ttccagatga
     2941 gtgaaacagc aattatcaaa caagttatgg ccgttcccat aaacacaata aaaaatatag
     3001 ggtttaatat tactctgtta atggactgca ttgcggcaat accctgagca ggtttaatac
     3061 tgccaagggc actcattaca gtgtttgaaa atataaaaaa cagtcctgcc ataagcccac
     3121 agccaatggc tgaatagagt gttaaaaaga ataaaatatt atccgacatg atttatagtc
     3181 tccagctata agatacctga acaggaattg tttgatatgg ataattagca ctgcaggata
     3241 ttgaaggtat gcttttaact gctttaaaat atgttgagaa gtataagctt taaactgagt
     3301 gtgtatatta ttattagatt cagttcactt ctcttctagg cacataactt tctcttacct
     3361 ggcttagctt tttaaaatat ggtaatagaa ttactgaata ccctcttgct tttcgacgct
     3421 atctctaaga ttcgtatcga ttcttataat atgattagtt agccaatttt ctgcaacatc
     3481 atgtacttcc attactagag attcagatgg gccctcagtt tttaatcttt gctgaaatcc
     3541 atcgaaagca ttcaggaatt tagtgtgagc ctctttattt ttatctgcaa aagggcattt
     3601 atattttttc atacatgctt cttctataga aaaatgtaat ttcacataac tactcatgga
     3661 ttttataaac acgccaatag tagcaagacc ctcacccttc tcaactttct ctgataattt
     3721 attgatctgt ctaaatatac ttttatgatg attatctata ttttcgagtc ctctacagta
     3781 gtcgtctttc catactaaca tctatataac ctccaaatat ataacaagga gctcgcatag
     3841 gacagcaaag tatactaagc tgaaactatg caaaagaagt tacttaagaa tacaaaacta
     3901 tcaaatagta agataagcaa gctaatacaa atgtttgtat tggaagtgcc gg
//
LOCUS       DBNGSV010000274         3422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_187720, whole genome shotgun sequence.
ACCESSION   DBNGSV010000274 DBNGSV010000000
VERSION     DBNGSV010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_187720"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1413)
                     /locus_tag="AC159U_10115"
     CDS             complement(52..1413)
                     /locus_tag="AC159U_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3337447.1"
                     /translation="MMMITRRIKSKKKADANNKFSDFYVSNSFNRFGKSKNNEKPGGN
                     ARKSDTDKKGSEDKEFEQFIQSDEFNSSIDESEFVSEVQSTEKEDKKDESKNKHWKPD
                     EEFRLLQVYFREMGIESLLSPKQEVEISAKIKLCEKNALILKSELEKILEQTFSDYPD
                     DLIVELCRIQNRSILNKLKQAGFANKSTSYDYRAKFKEVRTLLSLCISCIKESFRFKS
                     RFVNSNLRLVVSIARRYVNRGLPLPDLIQEGNVGLMRAVERFDHTKGYKFSTYASWWI
                     HQAISRSLLDQTRTIRVPVYVLEQATKVNRISTLLHKKNGKKPEPEEISKKSGISVEG
                     VKRVLGATKDVVRLDTPVMDGEKTSLMDFIPDENALTPDKAVATYTLSDNLNDALSSL
                     SPREEQILKMRFGINLETTYTLDEIGKYFSLTRERIRQIEKRALEKLEDSDNGGRLKS
                     YLY"
     gene            complement(1435..2721)
                     /locus_tag="AC159U_10120"
     CDS             complement(1435..2721)
                     /locus_tag="AC159U_10120"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR3337448.1"
                     /translation="MNQVKTINNNKSLQVIVNEISKHALDASRILSTVSSDIKNKTLK
                     SMANLLRGNSSFLIKENKKDLEYADNKGISKSMIDRLVLNESRIDSMALSLEEVSELA
                     DPVGEVTRMWTRPNGLRVGKMRIPLGVIGIIYESRPNVTSDASGLCIKAGNSVILRGG
                     SESLNSNLAIGAVLHKALKSNGLPEHAVQIIPVTDREAVMELLRQDEYIDLIIPRGGE
                     GLIRFVCDNSTIPVLKHYKGVCHLFIDEHALFDMALSIAVNSKVQRPGVCNSAETLLV
                     HKNIANKFLPLIYKEFTKNGVEIRGCKQTKTVLKEVTLATEQDWYEEYLDLVISIKVV
                     DSIDDAISHIEKYGSMHTESIVTESYTNSQYFMNRVNSSTVMTNASTRFADGFQLGLG
                     SEIGISTSKLHAFGPMGCEELTTTKFIVYGNGQIRN"
     gene            3089..3328
                     /locus_tag="AC159U_10125"
     CDS             3089..3328
                     /locus_tag="AC159U_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337449.1"
                     /translation="MNRFYKNNVLSVLLLVAVSLLISTDTSFAYIDPGSGSMILQVLI
                     ASILGILTAVKIYWHKIKSFFSKSSDSNITEDNKK"
BASE COUNT         1030 a          640 c          500 g         1252 t
ORIGIN      
        1 gtcaaaaata gcaaaatttt taattcttaa tgaaactagc attaagtaat actaatagag
       61 atagctcttg agcctacctc cattatccga gtcctctagt ttttcaagcg ctcttttttc
      121 aatttgcctt attctttcac gtgtgagact gaagtatttt ccaatttcat caagagtata
      181 agtagtttca agattaatgc caaatctcat cttaagaatt tgttcctctc ttggagaaag
      241 agacgaaagc gcatcattca aattatcact aagggtatat gttgctacgg ctttatcagg
      301 agttagggcg ttttcatcag gtataaaatc cataagagaa gttttttcac catccataac
      361 aggagtatcc agccttacta catcttttgt ggctcctaaa actcttttta ctccttctac
      421 cgaaataccg gattttttag atatttcttc aggttcaggt tttttaccgt ttttcttatg
      481 aagcagtgta cttattctat taacctttgt agcctgttcc aatacatata ccggaaccct
      541 tattgttcta gtctgatcaa gaagtgacct tgagattgcc tgatgtatcc accatgaggc
      601 ataggttgaa aatttatacc ctttagtatg gtcaaatctt tcaacggctc tcataaggcc
      661 aacattaccc tcttgaatta aatcaggaag aggcaaaccc ctgttaacat accttctggc
      721 tatactaaca accagccgca gattggagtt tacaaacctg cttttaaacc taaaagattc
      781 tttaatacag ctaatgcaca aagacagtag tgttcttact tctttaaatt tggctctata
      841 gtcataggac gtacttttat tggcaaagcc agcctgcttt aacttattta atattgatct
      901 gttttgaatt ctgcaaagct ctacaatcaa gtcatcggga taatcactga atgtctgctc
      961 taatattttt tctagttcac tttttaagat taaagcgttc ttttcacata gctttatctt
     1021 tgctgaaatc tctacttcct gttttgggct taagagtgat tcaataccca tctcacgaaa
     1081 atagacctga agcagcctga actcttcatc aggtttccag tgcttatttt tactctcatc
     1141 ttttttatct tctttttctg tggattgaac ctctgaaaca aactctgatt cgtctatact
     1201 tgaattaaac tcatcacttt gaataaattg ctcaaattcc ttatcttcag accctttttt
     1261 atctgtatca gattttcgtg cattcccgcc aggtttttca ttgtttttgg atttaccaaa
     1321 tcggttaaat gaattggaaa catagaaatc tgaaaatttg ttatttgcat ctgctttttt
     1381 ctttgattta attctcctgg ttatcatcat catcctcctg tgaaaattaa aatcttagtt
     1441 tcttatctgt ccatttccat aaactataaa ctttgtagta gtgagttctt cacatcccat
     1501 aggaccaaaa gcatgaagct tggatgtact tattccaatc tctgagccaa gaccaagctg
     1561 gaatccatcg gcaaatctgg tggaggcatt tgtcattaca gtggacgaat taaccctatt
     1621 catgaaatac tgtgagtttg tataactctc cgtcactata gattctgtat gcattgaacc
     1681 atatttttcg atatgtgaaa tagcatcatc aatactatct acgaccttta tacttattac
     1741 taaatcaaga tattcttcgt accagtcttg ttctgttgct aatgtcacct cctttaatac
     1801 tgttttggtc tgtttacaac ctcttatttc aactccattt ttagtaaatt ctttataaat
     1861 tagtggtaaa aacttattag ctatattttt atgcaccaaa agtgtctcgg ccgagttaca
     1921 gactcctggc ctttgtactt tggaattaac tgctatgctt aaagccatat caaatagcgc
     1981 atgttcatct ataaacagat gacaaacacc tttgtaatgc tttaatacag gaattgttga
     2041 gttgtcacat acaaagcgta taagaccttc tccacccctt ggaataataa gatctatata
     2101 ttcatcctgt cgcagaagtt ccattactgc ctctctgtca gtaaccggaa taatctgaac
     2161 agcatgctcg ggtagaccat ttgattttag agctttgtgt agaactgcac ctatagccag
     2221 attagaattg agtgattcag aaccacctct tagtattaca ctatttcctg ctttaataca
     2281 caaccccgag gcatctgagg taacatttgg tcttgattca taaataattc ctatcacacc
     2341 aagtggaatc ctcatttttc caactcttag tccatttggc ctggtccaca ttcttgtaac
     2401 ctcaccaacc ggatcagcga gctcggatac ttcttcaaga ctaagcgcca tagaatcgat
     2461 tcttgattca tttaatacaa gcctgtctat catggatttg gagattcctt tattatccgc
     2521 atattccaaa tcttttttgt tttctttaat taagaacgaa ctattacccc tcagaagatt
     2581 tgccatggat tttagtgttt tgttcttaat atctgatgaa acagttgaaa gaattctgga
     2641 tgcatccaat gcatgtttag agatttcatt aactatgact tgtaatgact tgttattatt
     2701 gattgttttt acttgattca taattaatca aatataaaga tgttacatta aagataataa
     2761 tatattgcta aataaaaagt caagctatat tttcagctta aaccatcatt tattactgaa
     2821 aaacggtatt atttcataat tacagtatgt tacaagacat atttattttc ttactttata
     2881 ttagatttta ggtattttta atgtattact tgaagtttat tatttttttt attaaatagg
     2941 caatttatta tatagatttc attccataat attatgggta tacaactgaa ttaagaaata
     3001 tatttctata ttaatgtttt cataactata atattaagtt aagattacct gattactttg
     3061 tagacatagt aaataggaga attaactcat gaatcgattt tacaaaaaca atgtattgag
     3121 tgtattatta ctagttgctg tttccttgct tatttctaca gatacttcct ttgcatacat
     3181 agatcccgga agcggcagta tgattcttca ggttttaata gcatctatac ttggtatact
     3241 tacggctgta aaaatctatt ggcataagat aaaaagcttt ttttcaaaaa gttcagattc
     3301 gaatataacc gaggataaca aaaaatagat tattttaccg aaactagctc atttagagat
     3361 ccgagcgctt ttatatttac aaaaaatagt atcacttacc gtcagattaa taatagttac
     3421 aa
//
LOCUS       DBNGSV010000275         4733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1712047, whole genome shotgun sequence.
ACCESSION   DBNGSV010000275 DBNGSV010000000
VERSION     DBNGSV010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1712047"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..940
                     /locus_tag="AC159U_10130"
     CDS             62..940
                     /locus_tag="AC159U_10130"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR3337450.1"
                     /translation="MKLWDILSLLRVHQWVKNLLIFAPPFFAGIFFNNINVFIEMILA
                     FFSFSLISSSTYIINDINDINKDREHPTKKNRPIASGRISIYLAVLILIFCFSSSVII
                     AMYFDTSFLLVLALYLLMGLAYSLFLQYIVILDTFTIAAGFVLRILAGGYASNTEVSS
                     WLLLTTFLVSVLLAFGKRRFELSLVSKNSKPFRKVLEHYTERFLDAGIIIFATTSIVT
                     FSIYAVEIGKKQFLISVPFACYGILRYLHIVQRENGADPTDAFIKDKALFVAVFLWIF
                     ASAVIVYFDDILSLFR"
     gene            complement(878..2635)
                     /locus_tag="AC159U_10135"
     CDS             complement(878..2635)
                     /locus_tag="AC159U_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337451.1"
                     /translation="MKLKNSLLVISLIIFISSIIVYLPSLKNDFVWDDEEVIENSHFT
                     FKASSIKDILIPKQNVDKKSKYYRPLHYSSIVFDKTVWGINSFGFHLTNILIHALNSL
                     LVFYLFRLVLKEFKIKDPEINAFVGSLFFILHPIHTESVSWVAGRTDTLCTVFFLAAF
                     IFHIKIQKRRIFITIALFTYFLSLLSKELAIAFPLVILCFEYLTNNLKRKNILVYCSY
                     FIITLIYLYLRGRAFVNIPEVEYISKNAADNTSLLIYFEPIKILFNSYFFYIYKLLFP
                     FELNAFISSVPVDTFYTLSSFVTITLITILVFYYGIKKHSLLLFSFLFFVLTLGPSAT
                     VALSNIAATPLAERYLYLPSVGFIFILVYLINQLKVRLKGNLNIIIISLLSVFYLFIC
                     IDRQAIWKNSLTLWQDTSKKSYTSPIVHTNYGSALLEAGMYEKAITELYIALSPEFND
                     TNRGRAVTSINLGNTYIALENYPLAEKWFIEANRYDPHYGRTYYQLGLINYIKAETTG
                     NKVYYKTAEKHLIETFKYYYSYSKAHLLLSNVYLGLGDKEKAKKQAEIALNIGLPGEL
                     SKKAEYIIKIDNNGRGKNP"
     gene            2818..3444
                     /locus_tag="AC159U_10140"
     CDS             2818..3444
                     /locus_tag="AC159U_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3337452.1"
                     /translation="MEAEYKQTEISKEIVSLSTKLFQQSGYKSFSYRDIAKEIGIKTS
                     SIHYYFPSKDDLALIIVKQYRKELAEELNNISSKNSDPHSRLDLYMDLYINSFRQNKT
                     VSFLSMLTMDLDNLPEIIRKEVFIIINDSISWIQKTIEDGYSKKVFHFDQSPGSLAQT
                     VFSALHGATITSRSINDESALLSTIESIKKMLATKDKGIISKYLSIGR"
     gene            complement(3505..4203)
                     /locus_tag="AC159U_10145"
     CDS             complement(3505..4203)
                     /locus_tag="AC159U_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337453.1"
                     /translation="MSQKGYDFSGTYQGEMDGISTSLNLTQKGSNLTGDINKGGLLYN
                     VVGIAEDDSIQGRMSDAENIEHYMLQAEINNSFLIIHLITHNLDDGSISKNSFTFKKS
                     NTKSADTIQPPEKVIKESEFNNDKSLLGIWAREEILTSPENAISTQVVIELDSKGNYS
                     LVGAINLKGAKDTGYSGDTTKGKWRTHNGTFHILVSGSKYWKPYARYKIDGPDEMTFT
                     LEDGERLNWVKQHK"
     gene            complement(4243..>4733)
                     /locus_tag="AC159U_10150"
     CDS             complement(4243..>4733)
                     /locus_tag="AC159U_10150"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3337454.1"
                     /translation="GCGSSMKEYSELLKDDTEYSDKAKEFSLKTKDIMEFISEIGLDA
                     KLKEVNKKLTYQDACHVAHGQKITTQPRELLKQIPGIEFCELKESDMCCGSAGIYNLV
                     QPEMSQKLLNRKVLNIKDIKPDILTAANPGCLLQITSGLNKEGINIETAHPIELIDEA
                     ME"
BASE COUNT         1516 a          759 c          798 g         1660 t
ORIGIN      
        1 taacaatagt tgcttttttc atcattttgc attataaatg agaagtcaat aggttagtct
       61 tatgaaattg tgggatattc tttctcttct aagagttcat caatgggtca aaaatcttct
      121 aatattcgcc cctccttttt ttgcaggaat ttttttcaat aatattaatg tatttattga
      181 gatgattttg gcttttttta gtttttcact aatttcctct tccacttaca taattaatga
      241 cataaatgat ataaataaag accgagagca tcctactaaa aagaacagac caatagcatc
      301 aggaaggatc agtatatatc tagcagtttt aatactcatt ttttgttttt cgtcttcggt
      361 aattattgcg atgtattttg atacaagttt tctactggtg ttggcacttt atctgctaat
      421 gggtctggct tattcactat tccttcagta cattgtcata cttgatacat ttaccatagc
      481 agcaggtttt gtccttagaa tcctggccgg tggttatgca tcaaacacag aggtatccag
      541 ctggcttctt cttactacct ttttggtatc agtacttctt gcatttggaa aaaggaggtt
      601 tgagctctct ttggtttcaa aaaattcaaa accctttaga aaagtactgg agcattatac
      661 agagaggttt ttggatgccg gaattattat ttttgctact acatctattg ttactttttc
      721 catatatgct gtagagatcg gtaaaaaaca gtttttaatc agcgtacctt ttgcctgtta
      781 cggtatactt agatatctac acatcgtgca aagagaaaac ggagcagacc ccacggacgc
      841 atttattaag gataaagctc tttttgttgc tgtattttta tggatttttg cctcggccgt
      901 tattgtctat tttgatgata tactcagcct ttttagatag ctcaccagga agaccaatat
      961 taagggcaat ttctgcctgt ttcttagcct tttctttatc accaagacct aaatatacat
     1021 tggagagaag aagatgtgct ttactgtatg agtagtaata tttaaaggtt tctataagat
     1081 gtttttcagc tgttttataa tatactttat taccggttgt ctccgctttt atatagttaa
     1141 ttagtcccag ttggtagtag gtcctcccgt aatgagggtc atatctatta gcttctataa
     1201 accatttttc agctaaaggg taattttcca gggcaatata tgtgttccct aggtttattg
     1261 atgtcacagc tctgcccctg tttgtatcat taaactccgg ggaaagggca atatataact
     1321 cagttatagc tttttcatac atccctgctt caagtagagc agagccatag tttgtatgca
     1381 ctatagggct tgtataggat tttttagatg tatcctgcca aagtgttaga ctatttttcc
     1441 agattgcttg tctgtctata caaataaaaa ggtaaaatac tgaaagcagg ctaataatta
     1501 ttatgttgag gtttcctttc aaccgtactt tcaattgatt tattaaatac acaagtatga
     1561 agataaaacc taccgatggc aggtagaggt atctttcggc aagaggtgtg gcagcaatat
     1621 ttgagagtgc aacagttgca gatggtccca aagttaaaac aaagaacaga aatgaaaaaa
     1681 gcaataaact gtgttttttg atcccgtaat agaacacaag aattgtaata agtgtaattg
     1741 ttacaaaaga gcttagagta taaaatgtat ctacaggtac tgaggatatg aaagcattta
     1801 actcaaatgg aaataagagt ttataaatat aaaaaaagta ggaattaaaa agtattttta
     1861 ttggttcaaa ataaatcagc aaagaagtgt tatctgctgc atttttactt atatattcaa
     1921 cttcagggat atttacaaac gcccttcccc gtagatacag atatataagc gtgattataa
     1981 aatagctgca ataaactaaa atgtttttac gtttaagatt atttgtaaga tattcaaaac
     2041 ataaaattac tagtggaaac gctatagcga gttcttttga aagaagtgag agaaagtaag
     2101 taaatagagc aatagtaatg aatattctgc gcttttgtat tttaatatga aaaataaatg
     2161 ctgctagaaa aaagactgta cagagcgtat cggttcttcc tgcaacccac gagactgatt
     2221 ctgtatgaat agggtgtaag ataaaaaata atgaacccac aaatgcattt atttcaggat
     2281 ctttaatttt gaactctttt agtacaagtc taaaaagata aaatacaaga agggagttta
     2341 gagcatggat taatatattt gttaagtgaa acccaaaact gtttataccc caaaccgttt
     2401 tatcaaatac gattgatgag taatgaagtg gtctgtagta tttagatttt ttatctacat
     2461 tttgtttagg tataaggatg tcttttatag aagaagcttt gaatgtaaaa tggctattct
     2521 caattacttc ttcatcatcc caaacaaaat catttttaag agaaggtaag tagacgataa
     2581 tggaagaaat aaatattata agagatatga ctaataagct attttttaac ttcatcgttg
     2641 tataagcaca tttatatttt gattatatag ttaacactaa tttatataag aataaaatac
     2701 tagcattaac tactttaata gttaaatcta aatattagac aatctcaata tatttattag
     2761 ttatgaatat tcaaattaaa acttgatgat ctatctataa gtagatagaa taaatatatg
     2821 gaagcagaat ataaacagac tgaaatctct aaagaaatag tttccttgtc aactaagcta
     2881 tttcaacaga gcggatataa atcatttagc tacagagaca ttgccaaaga aattggaata
     2941 aagacttcga gcatacatta ttacttcccc agtaaagatg atcttgcatt aataattgta
     3001 aaacaataca gaaaagagct tgcagaagaa cttaataata ttagcagtaa gaattctgat
     3061 cctcactcga gacttgatct ttatatggat ctatacataa acagttttag acagaataaa
     3121 actgtaagtt ttttatccat gcttacaatg gatttagata atctgcctga aataattaga
     3181 aaagaagtgt ttatcattat taatgatagt ataagttgga tacaaaaaac aatagaagat
     3241 gggtatagca aaaaagtatt tcatttcgat cagtcacccg gaagtttagc gcaaacagtg
     3301 ttttcagctc tgcacggagc taccataaca tccaggtcaa taaatgatga atctgctctt
     3361 ctcagcacaa tcgaatcaat aaagaaaatg cttgcaacaa aagataaagg tattatctca
     3421 aaatatctat ctattggtag atagatagtg taagttccag agattgataa aattttaaat
     3481 aattacatat aactgtaaat tggtttattt atgttgtttc acccaattaa gtctttcgcc
     3541 gtcttctaag gtaaatgtca tttcatcagg cccatcaatt ttatatcttg catacggctt
     3601 ccagtatttt gagccagata caaggatatg gaacgtcccg ttatgcgtgc gccatttacc
     3661 ctttgtagta tctcctgagt agccggtgtc ttttgcgcct tttagattta tggcgcctac
     3721 gaggctgtaa ttacctttgc tgtctaactc aattacaacc tgagtagaaa tagcattttc
     3781 agggcttgta aggatttcct ctcttgccca aataccaaga agtgatttgt cgttattaaa
     3841 ttctgattct ttaatgactt tttcaggagg ttgtatcgta tcagcacttt ttgtatttga
     3901 ttttttaaag gtaaatgaat tttttgagat gctaccgtca tcaagattat gagttataag
     3961 gtgtataatt aaaaaactat tattaatttc agcctgaagc atatagtgtt ctatgttttc
     4021 cgcatcgctc atccttccct gaattgagtc atcttctgca attcctacaa cattatataa
     4081 caatccgcct ttgtttatgt caccagtaag gttggacccc ttctgagtta aattgagtga
     4141 ggtgctgata ccgtccattt caccctggta agtaccactg aaatcatatc ctttttgtga
     4201 caattatatt actccttagt agttgcggta aattatctaa aactactcca tggcctcatc
     4261 tattaattca attggatgag ccgtttcaat attgattcct tctttattca gtcctgatgt
     4321 aatttgaaga agacatcccg gattggcagc ggttagtata tctggtttta tatcttttat
     4381 gttgagtact tttctattca atagcttttg agacatttcg ggttgtacaa gattataaat
     4441 acccgcacta ccgcagcaca tatcagactc tttgagttca caaaattcaa ttcccggtat
     4501 ttgttttaaa agttctcttg gttgtgtagt tattttctgt ccgtgggcta catggcatgc
     4561 atcctgatat gtcagcttct tgttaacctc ttttaatttg gcatctagtc caatttcact
     4621 aataaactcc attatatctt tagttttcaa actgaattcc tttgctttat cactatattc
     4681 ggtgtcatct tttagaagtt ctgagtactc tttcattgaa gagccgcaac ctg
//
LOCUS       DBNGSV010000276        19201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1642220, whole genome shotgun sequence.
ACCESSION   DBNGSV010000276 DBNGSV010000000
VERSION     DBNGSV010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1642220"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="AC159U_10155"
     CDS             complement(<1..795)
                     /locus_tag="AC159U_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3337455.1"
                     /translation="MKVLNKRELPDQRLSTLDETGKRVYIFPEKVRGLFRKWRTLTQV
                     ILITILLVLPWLKVNGYQALLFDIPNRRFSIFGLTFWAHDGPLIFYILAILTIGLAFM
                     TAVYGRVWCGWGCPQTVFVDGVFRRIEHLIIGSHIKQMNLAKAPWNQEKILKYSITWF
                     FFTLVSLIITHSFLAYFVGAERLVEMTQHNPQENWTIFVIMFFISSVILFDFGWFREQ
                     FCIIMCPYGRIQSVLMDDDSVTVAYDYNRGEPRKGTVDAANKAGDCI"
     gene            complement(797..1375)
                     /locus_tag="AC159U_10160"
     CDS             complement(797..1375)
                     /locus_tag="AC159U_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3337456.1"
                     /translation="MSNENQNEDKPIEGHEYDGIRELDNPLPGWWLTTFYLTVIFAVI
                     YWVYYQFGSGPTSEEYLMKDMESIQTLQAKFEDTMQVRSEDSLLALVNDQEALSAGKA
                     EYIIKCAACHGAEAQGLIGPNMTDDYWIHGDGSITSILKIANEGVLDKGMPPWKGVID
                     VKLLEKVSVYIYSIRGSNPPGAKPPQGEKITK"
     gene            complement(1372..1545)
                     /locus_tag="AC159U_10165"
     CDS             complement(1372..1545)
                     /locus_tag="AC159U_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase subunit 3"
                     /protein_id="HIR3337457.1"
                     /translation="MKQIALAYYEHTYLVAIALLIFFTAFIAIVLSTYRKSNEEIYKE
                     LEKLPLEKEENKS"
     gene            complement(1547..3700)
                     /gene="ccoN"
                     /locus_tag="AC159U_10170"
     CDS             complement(1547..3700)
                     /gene="ccoN"
                     /locus_tag="AC159U_10170"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR3337458.1"
                     /translation="MSEAKTTSASQLETFEYDDLIVKKFLTATIFWGAAAFIVGLLAA
                     LQLAFWQANLDLEWLTFGRIRPLHTNAAIFAFGGNAIFMGIYHSLQRLLKTRLYSDFL
                     SNVHFWGWQLIILSALITLPLGYSHGKEYAELEWPIDIAIAVVWVIFAVNFFGTIAIR
                     RIQHLYVAIWFYIATIITVAVLHIVNSIEIPVTMLKSYPVYAGIQDALVQWWYGHNAV
                     AFFLTTPFLGLMYYYIPKASNRPVYSYKLSIIHFWALVFLYIWAGPHHLLNSALPDWA
                     QTLGMVFSVMLWAPSWGGMINGLLTLRGAWHLLRTDPIIKFLVAAVTFYGMSTFEGPL
                     LSIKSVNSLAHYTDWIIGHVHGGTLGWNGFLTFGIIYFLVPKIWNTKIYSHKLVVNHF
                     WIALVGILLYYISMWASGITQGLMWQAIDDSGQLVYPDFVETVMRIVPLYYVRFIGGL
                     LFLIGYIMLIYNVWMTVKMAPKESKAETFTAPAPSHSSSDLGTGHRKIEGLGAVFTVL
                     VFVSIIVGSVIEIIPTLSIHKYVPANVATKPFTPLELEGRDIYISEGCYTCHSQMIRK
                     LQFDVLRFGPHSTIEESMYNRPFQWGSKRTGPDLARVGGKYPDLWHLRHFINPREISP
                     NSIMPSYPWLAVKKIDYLNSRKKLSVMKKLGVPYEQDVVANADIHAQKQADDILKGLV
                     EQDPSLKRIKDTQVLAMIAYMQSLGKKTEKIVQGE"
     gene            complement(3693..3875)
                     /gene="ccoS"
                     /locus_tag="AC159U_10175"
     CDS             complement(3693..3875)
                     /gene="ccoS"
                     /locus_tag="AC159U_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HIR3337459.1"
                     /translation="MNIIFFTLGVSLLLSLSFLLFFIWSTKKGQYDDLVTPSHRAILD
                     NEKQKRKLKTEDRTNE"
     gene            complement(3872..6103)
                     /locus_tag="AC159U_10180"
     CDS             complement(3872..6103)
                     /locus_tag="AC159U_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3337460.1"
                     /translation="MINTTTSKLEDYKYLDNSNFKDLYTSADSPLRMSFYIEGVHCSG
                     CLGVLEEIPSKSDFIKSVTLNMSTNIADVEFSSADCYSKFPQLVSNLGYKAYPIKDSS
                     DAKNIKKLENKDSLYRIGISGVCAGNIMLLSAAIYSGAEGIFRNNFEIINLFLSIPVV
                     TYCSFPFYKNAYSSIRALKATVDFPVVFVVLIGFILSTYNLIKGIGGVYYDSITIFVF
                     LLLVSRYLIRSFQNKLISDTDLFSSLLKDNKILKYDTHTNTYGFYPISELKSGDLVKL
                     DRGQRVPVDGILLNHIAVFDLSVLTGEAIPKTVLENSDVFAGSILDSEQAELKVINTG
                     IETRLGKVLDKVKENYEQKISFEGFSDRYSTIFTSFVAVVSIAGFFSIANIYGTHEAL
                     KRIVSFVLIACPCAFVFILPLTFGLSLKSAVKKGFIIKDSNIFEKIRSVRNVVFDKTG
                     TLTKGKFNIVQWDKKNLSAEDLSAILAIESKSHHPVARAIVSSLTLDRLNLPEVMNLE
                     NIHSRGIKAEVGGHTYEIYSDDKSAKIYNSASAVSNCVSVIKDGQKLSTIYLGDEIKD
                     DAFDTLNQLSKDKYDIYMLSGDRKGNVELVSSMLSIPTGNSLSGLTPEQKIDFIKTKK
                     GSMFVGDGLNDAGALLNADIGIAVQGSAEQSLKVSDIYILRNSLSSILEIIKHANITN
                     STIKTNTILSVIYNVSAGMLALMGYINPLLAAVLMPLSSVLLLGSSLIGMSKNKAGGL
                     QTK"
     gene            6515..7171
                     /locus_tag="AC159U_10185"
     CDS             6515..7171
                     /locus_tag="AC159U_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337461.1"
                     /translation="MEKSFRFILVALIATCFFAASGVRSFAEQPMGGEFALTFGAQTG
                     PFEMGTGWHLAGEIGLPLMMFSSGKLMGLVNIGVSNAEDDISVEPTVNALSPGLLPTQ
                     TTIDLTTAVIVLGLKYKHTAHDIIQPYVLAGPGIDIFFNDTEPGDLPGGIAPQPEEIR
                     QRGFPGGQGNVELGLHAGGGIDINITEKIFIGAEGRFNWVDRDNGSYGTYGGRLGFRF
                     "
     gene            7287..7856
                     /locus_tag="AC159U_10190"
     CDS             7287..7856
                     /locus_tag="AC159U_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3337462.1"
                     /translation="MATEKISSKDKILKAALTLFTKKGIKSTTTKELAKKAKVAEGTI
                     YIHFKSKEEIAHYLFKHYMGVFTQHLTQFSDGITDPGNKMKALINAFFAYAEKEPTAI
                     YYIVIAHYTELNVLKKEKFTLRDIFADTIENGVKKKQFSKIDKNLGAALVVGMINMAV
                     LSYNNGFIDLDYNKIASDTNKAALKLLSR"
     gene            8083..8322
                     /locus_tag="AC159U_10195"
     CDS             8083..8322
                     /locus_tag="AC159U_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337463.1"
                     /translation="MADNGLYFLDAVYNSTRDAWEIGIAYMVNDEDVSGRYKNIKITV
                     NIPGLRKDEPKAKQLAVQKTRQFLMEAAHAPLEDE"
     gene            complement(8340..9221)
                     /locus_tag="AC159U_10200"
     CDS             complement(8340..9221)
                     /locus_tag="AC159U_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3337464.1"
                     /translation="MYKLRKILWATDGSKQSVNALKYSKKISEKFNSKTIGIHVIRDL
                     NKKIFDILNMKLNFEEMIAGERDTYGKFFVKIEKEFNKQNRVFEHYIACGKTDEEVLT
                     YSNKNKIDLIVLGHKGASSDEGMFSGSSTNKIVRSADIPVLVVNMNYKKRLSNIKRIV
                     VPINLTEDLVSALDYAVFLASTLGAEVSVLYVQEFISYSYEMPIIILDDMKKYFDNKL
                     NKLTKKYKNKNVKISTHVTEFLNPYMGILRHCEKIRPDLIVMNTHERKGLKKYFLGSV
                     TEKIINDMPCPVLTLKP"
     gene            complement(9326..10567)
                     /locus_tag="AC159U_10205"
     CDS             complement(9326..10567)
                     /locus_tag="AC159U_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3337465.1"
                     /translation="MKENDSNGVIKLNRNIPLWLITFYGIGTILGAGIYVLIAEISSQ
                     SGNFTPFSFLISAVIVSFSAFSYSELSSRYPRSAGEAAYVQQAFRAKWLSSFVGWAIV
                     IIGIVSSATIAKGFVGYFQVLFNVPDWLCISMLVFVLCLISIWGVSLSLKAAAVMTVV
                     EIIGIVLVIYFCGNNLSAIKTNVTDFIPSSDIKTWGNIFVGAFIAFYAYVGFEDIVNM
                     AEEVKNPRKNLPIAIIIALVVTTVLYIIVSLITVTSLPINILSNTRSPFTTLIELNSN
                     FPVSIITVISIMAIVNGALIQIIMGSRVLYGMASQKISLKIFGHINKHTKTPDYATVF
                     VGIVVLVLALWFPIVTLAKASSLIILLVFMLINLSLFIIKARKKEEEQSYIRLPIIIP
                     VLGFGLCLFLLMAQLVNKFTG"
     gene            complement(10610..11179)
                     /locus_tag="AC159U_10210"
     CDS             complement(10610..11179)
                     /locus_tag="AC159U_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3337466.1"
                     /translation="MIKIFYILIISIPILLSCSTGLTSQDVDKTTSDIEKAKHLVSEY
                     GCISCHTPKIETTVGLIPDPNRLLSGHSQSDENLPNIPEGLIGDKLWAGIYSKSLTAW
                     AGPWGIRFAANLTPHKETGIGNWSKQNFISTIRLGIHTTSLARELVPAMPWRDISKLS
                     DDDLGAIYSYLMSLRPVNNKVPEPVEPKN"
     gene            complement(11190..12050)
                     /locus_tag="AC159U_10215"
     CDS             complement(11190..12050)
                     /locus_tag="AC159U_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3337467.1"
                     /translation="MFSKILLGYDGTHESEQVLKVSANIARKFGSEIRAVNILPEHTE
                     FTKSFSVSERNLFDHWVEDNLKKKNIKKLEKKKEQLSNQGIKFSYEINYGVPHKEILK
                     EIKKNNYNLVVMGKGRFGGQSILGGTAKKILRDSNVSIFVHNTDFKKSTFKKVLVPTD
                     IYNIAKKDLRYASEISEKYNSKVYLLNIVEKGDNNYPAEVIETLKGNSYTALSEYLLK
                     SKNMSGVEPRVRVAQNSWVGIKDFIVEENIDLVVMMSYGGDKIRSEYLGSVAEKVVEH
                     SPCPVIALQL"
     gene            complement(12148..12600)
                     /locus_tag="AC159U_10220"
     CDS             complement(12148..12600)
                     /locus_tag="AC159U_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3337468.1"
                     /translation="MALTRWKPKNMEKWFDEFFEEPYFPQGWMRMPALRKIKSIEEFS
                     PVVDMYDNKDEIVVKAEMPGMKKEDVHVTTTEDSITIKGEMKKEEEVKEDDYYYSERS
                     FGSFSRMLHLPSKIKTNKVEAKFDNGLLEVHMPKADDSKPKEVKISLK"
     gene            12847..13431
                     /locus_tag="AC159U_10225"
     CDS             12847..13431
                     /locus_tag="AC159U_10225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3337469.1"
                     /translation="MKQYPRTYTTRQNVKLTVRPRTEDDSEYLSLFFLDIPKEDLIIY
                     REDVYKSHSIETWFLDESYKKDVQLLALSDNEIIAEGTIHKEGIYWQNAAEIKLIVKP
                     DYRNQGIGSYMFNSLLHEFLERKLQKVIVRYTPDNMSFIKIINRYGFKPETVLKSYVK
                     IDKDDKEQDLIIASYNLVDWIRKFEFYNLITPRR"
     gene            13633..16059
                     /locus_tag="AC159U_10230"
     CDS             13633..16059
                     /locus_tag="AC159U_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3337470.1"
                     /translation="MAKKSSQTHNISILNNKLKREIEKRTRAENALKHTLSLIKKKTS
                     IKKIINTVLQSVHKSVDPQTVMKNAVSSMSRNIENADNVSIYMVEAKEAVLKANRGYP
                     KSFIKKVSVIPYPKGFTWETLRTGKLIYCDDTEKDKFMGPAGIEIGTKSYASMPIKLG
                     RKTIGVININSLKKEAFGKEDLNMLKLVAEQIQTALNNARHAEQLQKEKDTAQGYLDI
                     SEVIILVLNDKGKVELINKKGCKILGFKHDEIIGRNWFDNFLPQSIKSEVKSVYKKVL
                     QGKLKTAKYYENIVVTKSGRERIITWHNTRLLKDGKAIGTLSSGVDITDLRLAEQRFE
                     QVVESSPNGFVMVNPKGRIVLVNRETEKLFGYHRSELMGQTVEILIPGQVRASHMDYR
                     KNFFLNPSNRAMGEGRDLFAVRKDGTRFPVEIGLSTLQTESGVAVLSSVVDITERKNA
                     EKLVKDNEQKLKAILDSTTDAILVYDKNGSIMIINNQAKKMFSENEKILENISQIIPA
                     SHRSIHNFQLSEAENGTNLFDYETERVLSNGKNIHASIALSYIPAQGGMYFESIRDIS
                     ERVIMRHKIIDFEKAQIIASMAEGIAHHMGTPLASMLLRVQMLKEDIEQFDNNDYFSQ
                     KLESIEKQIHYGQKVMQRLLRFASKPTSERVPSNIKNIASDAVEILKPLLNKKRIKLV
                     IDINKDYMILADGNMIQLVFSDLIINSIHAIENEGTIKVTASNDESNDNLTIKISDTG
                     SGIPEEVIPYVFEPFFTTKTTEQGTGLGLAVAKRIVHEHDGEIMIESKKNIGTDIIIN
                     LALYKGDTKS"
     gene            16056..17408
                     /locus_tag="AC159U_10235"
     CDS             16056..17408
                     /locus_tag="AC159U_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3337471.1"
                     /translation="MSTFKVLIVDDDQEYVNTLKELLERNHITVIAAYSGEEALEMFT
                     NNPDIRLALVDLIMPMMDGITLMEKLKEKNSELNIIMITGQGTVTNAVEALKKGANDF
                     ITKPFDKDILLSKLEIYRKSYELENKVENLSSIISTKYGFDKIVSSSKSMKTVFEKAA
                     AAARTDATVFLVGETGTGKELLAKSIHIKSDRSRYPFIPINCGAIPKELLESELFGHK
                     KGSFTGADKDNQGLFLAAQRGTILLDEIGEMSKDLQVRLLRVLQEQKIRPIGGSNEIP
                     IDTRVIAASNHSIEDLKNKELREDLFFRLAVIVIEIPPLRDRKEDIPILINHFINIFN
                     SKYDKNISGVNEDVLSILYNYDFPGNIRELENLFQGIVVLAPEDKKFINEKDLKASLV
                     WHDTKTSTKSVLSLEKVERFAIENAIRQAKGNKSKAAELLGVSRDTLYRKLKQYNLEE
                     "
     gene            17571..17996
                     /locus_tag="AC159U_10240"
     CDS             17571..17996
                     /locus_tag="AC159U_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3337472.1"
                     /translation="MIKKIVFPTDFSEYSSKARDYVAELAGTLGSRVYIIHAIEPIQY
                     EEDDEEIKQFYRNIELEINNKIEKEKDYFLQKNIDVYTDTVIGSAWKVINTYAKEKEI
                     DLIVMGSHGFKTEKDEVVIGTTSHKVVLTSPCPVLIVRN"
     gene            18012..>19201
                     /locus_tag="AC159U_10245"
     CDS             18012..>19201
                     /locus_tag="AC159U_10245"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3337473.1"
                     /translation="MNRLFIDDMPDSFYKDKKVLVRVDYNVPAIRGQIKEDYRIRQTI
                     PTIEYLSERGAKTILISHLGRPGGTPDKKLSLKPIAERLVEILKVRNVKFVDQCIGNH
                     VNYLADHLHKGEVLLLENLRFLNDEEDNDPEFCRLFSDIADIYVNDAFSTSHRTHAST
                     YGIPMLFEHKLGGFVLKNELYNLDKIKENPKRPFLAIVGGSKGKEKIGAISSLVKYAD
                     TILIGGCVANTFLAARGISVGDSNIANKSIEQAKTILRDYADKIILPDDFLVEDCDRR
                     EILKNVHYEIPLGQKVVDIGRDTTYYYSHQILNNNGTIFWSGPMGQFEVDEYAHGTNE
                     IAKSVSYAHWRGAYTVVGGGDTIASIRKADVLLNEIDFVSTGGSATLKYLAGINLPGI
                     SVLS"
BASE COUNT         6382 a         3556 c         3396 g         5867 t
ORIGIN      
        1 aatacagtct cctgctttat tagctgcatc tacagttcct tttctgggct ctcccctgtt
       61 ataatcatat gcaactgtta cagaatcatc atccataagg accgactgaa tccttccata
      121 aggacacata ataatgcaga actgctctct aaaccatccg aaatcaaata aaattacaga
      181 tgagataaaa aacattatta caaaaattgt ccagttttcc tgagggttgt gttgagtcat
      241 ctcaacaagt ctttcagcac ctacaaaata tgccagaaaa ctgtgtgtga taatgaggct
      301 aaccaaagta aagaaaaacc aggtaattga atattttaat atcttttctt gattccaggg
      361 agcttttgca aggttcatct gctttatatg gctgccaatt atcaggtgct ctattcttct
      421 gaatacccca tctacaaaaa ctgtctgagg acaaccccac ccacaccaaa ccctcccgta
      481 tacagcagtc ataaaggcaa ggccaatggt aagaattgcg agtatataaa aaattaaagg
      541 gccgtcatga gcccagaatg taagaccgaa gatcgaaaac cttctgttgg gtatatcaaa
      601 taatagagcc tggtatccat ttacctttaa ccagggtaaa acaagaagaa tagtaatcag
      661 tataacctgt gtcagggttc tccattttct aaaaagtcct ctaaccttct ccggaaatat
      721 atataccctt ttgcctgtct catccagtgt ggacaggcgt tggtcaggca actctctttt
      781 attgagaacc ttcattttat ttggttattt tctcaccctg aggcggtttt gcccccggag
      841 gattagatcc tctaatacta taaatatata ctgatacttt ctctagtaat tttacatcaa
      901 tcacaccttt ccacggaggc atacctttat ccagaacacc ttcattggca attttaagta
      961 tagatgttat tgagccgtca ccatgtatcc agtaatcatc ggtcatatta ggaccgatca
     1021 gtccctgggc ctccgctcca tgacaagctg cacattttat aatatattca gcttttccag
     1081 ccgataaagc ttcttggtca ttaaccagag cgagcagact atcttcggat cttacctgca
     1141 ttgtatcttc aaacttagcc tggagggttt gtattgactc catatctttc attaaatatt
     1201 cttcagatgt tgggcctgag ccaaattgat aatagaccca gtaaattaca gcaaatataa
     1261 cagtcaggta aaaagtcgta agccaccatc cgggtagagg attatcaagc tcacgaatac
     1321 cgtcatactc atgaccttca attggcttat cttcattttg gttttcattg ctcatgattt
     1381 attttcctcc ttttctagag gtagtttttc aagttcctta taaatctctt cattagattt
     1441 tctatacgtg gaaagtacta tggcaataaa tgcagtaaag aaaatcaaaa gtgcaattgc
     1501 cacaagatat gtatgctcat aatatgcaag tgcaatctgt ttcattttat tctccttgta
     1561 ctatcttttc tgtttttttg ccaagactct gcatgtaagc aatcattgca agtacctggg
     1621 tatcttttat ccttttaagc gatggatctt gttccacaag acctttaaga atatcgtctg
     1681 cctgcttctg tgcatgaatg tcggcgttgg ctacaacatc ctgttcataa ggaacaccga
     1741 gcttcttcat tacgcttagt ttctttctgc tatttagata gtcgatcttc ttcacggcaa
     1801 gccatgggta tgaaggcata atagaatttg gagagatctc tcttgggtta ataaaatgcc
     1861 tcagatgcca taggtcggga tatttgccgc ccaccctagc taaatcaggc ccggtcctct
     1921 tggatcccca ctgaaatggt ctgttataca tggattcctc aatagtagaa tgaggtccaa
     1981 atctgagtac atcaaactga agcttcctta tcatttgaga gtgacacgta taacaccctt
     2041 cgcttatata gatgtctctg ccctcaagct caagaggcgt aaacggtttg gttgcaacat
     2101 ttgcgggtac gtatttatgt atagaaagtg tgggaatgat ttctatcaca gaccccacta
     2161 tgatggacac gaatacaaga actgtaaaaa cagctccaag cccttcgatt tttctgtgtc
     2221 cggttccaag gtctgaactg ctatgacttg gtgcaggtgc ggtaaaagtc tcggcttttg
     2281 attctttagg agccattttt actgtcatcc atacattgta aataagcatg atatagccaa
     2341 taaggaataa caatccacct atgaatctga cataataaag aggcactatt ctcattacgg
     2401 tttcaacaaa atccggatat acaagctgcc cggaatcatc aattgcctgc cacatcaacc
     2461 cctgtgtaat accacttgcc cacatagaaa tgtagtaaag cagtattcct acaagggcaa
     2521 tccagaagtg atttaccaca agcttgtgac tatagatttt tgtgttccat atttttggta
     2581 ccagaaaata aattattcca aaagtaagaa atccattcca tccgagtgtt ccgccatgaa
     2641 cgtgccctat gatccagtct gtgtaatgtg caagagaatt tacagacttt attgatagaa
     2701 gcgggccttc gaaagttgac attccataaa aagtaactgc cgctaccaaa aacttgatta
     2761 tcggatctgt acgaagcagg tgccatgcgc ctctaagcgt aagaagacca tttatcatcc
     2821 ccccccagct aggggcccaa agcattacgc tgaaaaccat acccagagtt tgtgcccagt
     2881 cgggaagagc agaattgagc agatgatgtg gaccagccca aatgtaaaga aatacaaggg
     2941 cccagaaatg aataatagac aacttgtaac tatatacagg tctgtttgat gccttcggaa
     3001 tataataata catcaaaccc aaaaatggag tcgtaaggaa aaaagcgact gcgttgtgcc
     3061 cataccacca ctgaacaagc gcatcctgaa tacctgcata taccggataa ctctttagca
     3121 ttgttactgg tatttcaatt gaattaacta tgtgcaaaac ggctacagtg ataatagttg
     3181 ctatataaaa ccatattgcg acataaagat gctgaattct tcttatagct atggttccaa
     3241 aaaagtttac tgcaaaaatt acccatacca ctgcaattgc aatatcaatc ggccattcaa
     3301 gctctgcata ttcttttccg tgtgagtaac ctagcggaag ggtaattaga gcagatagaa
     3361 taattaactg ccatccccaa aaatgaacat tggataagaa atctgagtat agtcttgttt
     3421 tcagaagcct ttgcagggaa tgatatatac ccatgaatat ggcatttccg ccaaatgcaa
     3481 atattgcagc atttgtatga agcggtctta tacgtccaaa ggtaagccat tcaaggtcga
     3541 ggttggcttg ccagaaagcc agctgcagtg ctgcaagcaa gccaactata aatgctgccg
     3601 ctccccagaa tatagttgct gttaaaaact tcttaacaat cagatcatca tactcaaatg
     3661 tttccaactg agatgcagat gtggttttag cctcactcat ttgttctatc ctccgtcttt
     3721 aattttcttt tttgtttttc attatccaat attgccctgt gagagggtgt gacaaggtca
     3781 tcgtactgtc ccttttttgt tgaccatata aaaaacagta gaaatgataa tgacaaaaga
     3841 agcgatactc ccagagtaaa gaaaataatg ttcatttggt ttgtaagccc cctgctttat
     3901 tcttagacat gcctattaag gagctgccta ggagaagtac agagctaagt ggcattagaa
     3961 cggctgccaa aagagggttt atatatccca ttaatgccag catgccggca gatacgttat
     4021 atatcactga aagtatagta ttagttttga tagtgctgtt tgtaatattt gcgtgcttaa
     4081 tgatttccag tattgatgag aggctgtttc taagtatata aatatctgag actttgaggc
     4141 tctgttcagc acttccctga acagcaattc ctatatcggc atttaaaaga gcccctgcat
     4201 cgtttaggcc gtctccaaca aacattgagc ctttcttggt tttaataaaa tctattttct
     4261 gctcgggagt tagtccagaa agcgaattgc cggtgggaat tgagagcatg cttgatacaa
     4321 gctcgacatt accctttctg tctccagata gcatataaat atcatatttg tccttggaga
     4381 gctgatttaa ggtgtcaaaa gcgtcatctt ttatttcatc tcccagataa atagtcgata
     4441 atttctgtcc gtcttttatt accgatacac aatttgaaac agcagatgca gaattataaa
     4501 tctttgcact tttatcatct gaataaattt catatgtatg gcctcctacc tctgccttaa
     4561 ttcctctgga atgaatgttt tctaaattca ttacctcagg aagattaagc ctgtctaaag
     4621 tcagactact aactatcgct ctagctacgg gatgatgaga cttactctca attgccagga
     4681 ttgccgaaag atcttctgct gataaattct ttttatccca ctgtactata ttgaatttac
     4741 cttttgttaa ggtaccggtc ttatcaaaaa caacatttct aactgaccta atcttctcaa
     4801 atatatttga atctttaata atgaatcctt ttttaacagc ggacttaagg gagagtccaa
     4861 acgtaagcgg gagtataaaa acaaatgcac atggacaggc aatgaggaca aaagagacta
     4921 ttctttttaa cgcttcatgg gttccatata tgtttgctat agagaagaac ccggcaatgg
     4981 atacaaccgc aacaaaagac gtaaagatag ttgaatatct gtcactaaaa ccctcaaagc
     5041 ttattttttg ttcatagttt tcttttacct tatcaagaac tttgccaagc cttgtttcaa
     5101 taccggtgtt aattactttt agttctgctt gctcagagtc cagtattgat ccggcaaaga
     5161 catctgaatt ttctaagaca gtttttggaa ttgcctctcc cgttaaaacc gagagatcaa
     5221 atactgcaat gtgatttaat agtatgccat ccaccggcac tctctgtcct ctgtcaagct
     5281 ttactaaatc tcctgatttg agttcagaaa taggatagaa cccataagtg tttgtatgag
     5341 tatcgtattt aagaatctta ttatccttta gaagtgagct aaacaagtcg gtgtctgaga
     5401 ttagcttgtt ctgaaaagac ctaataagat atctgcttac aagaagcaga aatacaaata
     5461 tggttattga gtcatagtaa accccgccta taccttttat taaattataa gtgcttaaaa
     5521 tgaatccaat taatacaaca aatactacag ggaaatcaac tgtagcttta agggctctta
     5581 ttgaggaata ggcattttta taaaatggaa aactacagta tgttacaaca ggaatggata
     5641 aaaacagatt aatgatttca aaattatttc taaaaattcc ctccgctcct gagtaaatgg
     5701 ccgcagaaag aagcatgata ttacctgcac ataccccaga tatccctatg cggtataaac
     5761 tatccttgtt ttctagtttc tttatatttt ttgcgtcact actatcttta attggatatg
     5821 ccttatatcc aaggttactt acaagttgag ggaatttcga ataacaatca gcacttgaaa
     5881 attccacgtc ggctatgttt gtagacatat tgagggttac gcttttaata aaatcagatt
     5941 tagaagggat ttcttccaat actcccagac atcctgaaca gtgaacaccc tctatataga
     6001 agctcattct tagaggtgaa tctgctgatg tatatagatc tttaaaattt gaattgtcca
     6061 gatacttata atcttccagt ttactagtcg tagtattgat catatgtgta gttatattta
     6121 tttgttctag aactcaagaa acgtctttga gaagaataaa agcaataaat atgccagtat
     6181 gtcttggcta gatttcagtc aagagcatgt gggttaatta tttattgata tactatttac
     6241 caaatagtaa tcactaagca agtctgctat ttctttataa ttacatattt attatatttt
     6301 gaatttaaca gtatttgcaa tttcaacagt tgtctgatat taagacatca gacataaatc
     6361 cctcaggcga gatcccttaa tatttatgaa atactcaatt taattgttgg catggacatt
     6421 gcacttaata tatttatgag tgagtgctca cttctttgcg tggtcagact atataatggc
     6481 agcagtcact taaaaatatt atggaggcaa gattatggaa aaatcattta gatttatttt
     6541 agttgcgtta attgcaacat gtttttttgc ggcttcaggt gtacgttctt ttgcggagca
     6601 acccatgggc ggggagtttg ctctaacatt tggcgctcaa acaggacctt ttgaaatggg
     6661 taccggctgg caccttgctg gtgaaattgg ccttccgctt atgatgttca gctctggaaa
     6721 actaatgggt cttgttaaca taggcgtttc taatgccgaa gatgatatct cagttgaacc
     6781 taccgtaaat gccctatccc caggcctact tcccacacag accacaattg acctcactac
     6841 agctgttata gttttgggcc tgaagtataa acatacggca catgatatta ttcagccgta
     6901 tgtacttgca ggacctggta ttgatatatt ctttaacgat actgagcccg gtgatctccc
     6961 cggaggaatc gcacctcaac cagaggaaat aagacaaaga ggatttccgg gtggacaggg
     7021 taatgtggag ctcggtctac atgcaggagg tggtatcgat attaacatca cagaaaaaat
     7081 atttatcggt gccgaaggca ggtttaactg ggttgacaga gataacggtt cttacggtac
     7141 ttatggtggc agattaggtt ttagattctg atatatcaaa tataaataac aaagaagggg
     7201 tgtattgtat ttagcaatac accctcccca ttccaaatta agcttactat ttgcaaaacc
     7261 caatacaaac aattatttta ttatccatgg caacagagaa aatcagtagc aaggataaaa
     7321 tattaaaagc agcacttaca ctatttacta aaaaaggcat taaatctaca actacaaaag
     7381 agcttgctaa aaaagcaaag gttgccgaag gcactatata tattcatttt aaaagcaaag
     7441 aagagattgc tcattattta tttaaacatt atatgggggt ttttacgcaa catctgactc
     7501 aattttctga cggcataacc gatcctggaa ataaaatgaa agctcttata aatgcattct
     7561 tcgcttatgc ggaaaaagag ccaacagcaa tatattacat agttatcgcg cactacacag
     7621 agctcaatgt attaaaaaaa gaaaaattta cactaaggga tatctttgca gatacaattg
     7681 aaaatggtgt taagaaaaaa cagtttagta agatagataa aaatcttgga gcagctcttg
     7741 tagtcggcat gataaacatg gctgttcttt cttataataa tggcttcatt gatctggatt
     7801 ataacaaaat agcctcagat acaaataaag ccgccctaaa attactgtcc agatagatgt
     7861 tccgtgttat atagatttat aaattgttta attatttgaa ctataataac tttatatttg
     7921 ctatagagtg agcgctcaca ttattacaac ttctacaagt aatgatgtgt ttgattgtaa
     7981 gacaccggac attttgaatg caatttatat aaggatgttt gtagattaga tggcacatat
     8041 tttgtaatat taatatctag ataattcact aaggagaatg atatggcaga taacggactt
     8101 tattttttag acgcagttta caattcgact cgagacgcat gggaaattgg aattgcctat
     8161 atggtaaatg atgaagatgt tagcggcaga tataaaaata taaagataac cgtgaacata
     8221 ccggggctta gaaaagacga gcctaaagca aaacagcttg cagtacaaaa aacaaggcag
     8281 tttttaatgg aagcagccca tgccccactg gaagatgaat agaatttgat gaatataact
     8341 taaggtttta gtgtaagaac agggcagggc atatcattaa ttattttttc agtcacactt
     8401 cccaaaaagt attttttgag gcccttcctt tcatgagtat tcatcacaat aaggtctggt
     8461 cttatttttt cacagtgccg caaaataccc atataagggt ttagaaattc tgttacgtgc
     8521 gtggaaattt ttacattttt atttttatat ttttttgtaa gcttatttag tttgttgtca
     8581 aaatattttt tcatatcatc cagtataata attggcattt catatgaata agatatgaac
     8641 tcctgcacat acaaaaccga tacttcagcg ccaagcgttg atgccaagaa cacggcataa
     8701 tcaagagctg acaccaggtc ttcggttaaa tttattggta caacaattct tttaatatta
     8761 gataaccttt tcttataatt catatttacc acaagtacag gtatatcagc agatctcact
     8821 attttgtttg tactgctgcc agagaacata ccctcatccg aagaagcacc tttgtgtccc
     8881 aggactatta agtcaatttt atttttatta gaataagtca gtacttcctc atcggtcttt
     8941 ccacatgcaa tgtaatgttc aaatactcta ttttgcttat taaactcttt ttcgatttta
     9001 acaaagaatt tgccatatgt atctctttcg cctgctatca tttcttcgaa gtttaatttc
     9061 atgtttaaaa tatcaaatat ctttttgttt aaatctctaa ttacatgaat ccctatggtc
     9121 tttgaattaa atttctctga gatttttttg gaatatttca atgcattaac agactgtttt
     9181 gatccgtcgg ttgcccataa tatctttctt agtttataca tgtgtccccc gtttaaacta
     9241 tatttactaa tgtgtgttaa ttgttctcaa ttataataat tgcaacattt gtgccatttg
     9301 aaatcaaaat gtaaaaccgt aaattttaac ccgtaaactt atttactaac tgggccatta
     9361 gcagaaataa acacagccca aacccaagaa caggaataat aatcggaagt ctaatataag
     9421 attgctcctc ttctttcttg cgtgctttta tgataaatag agatagatta ataagcataa
     9481 atacgagcaa tataattaaa ctcgatgcct ttgcaagtgt aacaatcggg aaccataatg
     9541 caagaacaag cacgactatt ccaacaaaaa cagtggcgta atcgggggtt ttcgtatgtt
     9601 tatttatatg accaaaaatt tttagtgata ttttttgcga agccattcca taaagcactc
     9661 ttgaacccat gattatctgt attaaagcac catttactat agccatgata ctaataacag
     9721 taataataga aacgggaaaa ttgctgttta attctataag agttgtaaaa ggtgaacgag
     9781 tattacttaa aatattaata ggcagggaag ttaccgtaat gagtgataca atgatatata
     9841 ataccgtagt aacaactaag gcaattatta ttgctatagg caggtttttt cttggatttt
     9901 ttacctcttc tgccatattt acaatatcct cgaatccaac ataggcatag aatgctatga
     9961 aagccccaac aaagatgttg ccccatgttt ttatatcaga cgagggtata aaatcggtaa
    10021 cattagtttt tatagcactc aggttgttgc cgcagaaata aatgacaaga actattccta
    10081 tgatttctac aacagtcatt acagccgcag ctttcaatga taggcttact ccccatatag
    10141 agattaagca tagaacgaat accaacatac taatacaaag ccagtccggc acattaaata
    10201 aaacctggaa ataacccaca aatccttttg caatagttgc tgaagaaaca attcctatta
    10261 tcactattgc ccagcctaca aaagaagaca gccattttgc acgaaatgct tgctgtacat
    10321 atgctgcttc tccggcactt cggggatacc tgctggaaag ctctgaatat gaaaaagcgc
    10381 taaaactcac aataactgca gatatcagaa atgaaaacgg ggtaaaattg ccactttgtg
    10441 atgaaatttc tgcaattagc acataaatgc ctgcccccag gatagtgcca ataccataaa
    10501 atgtgattaa ccaaagtggt atattccggt ttaatttaat tacaccatta ctatcgtttt
    10561 ctttcatcag ccgctatata aaatagattt atggagagaa atatatctat tagttttttg
    10621 gctcaacggg ttctggcact ttattattga cgggtctcaa agacattaga taactgtata
    10681 ttgccccaag atcatcatca cttagtttgg atatgtccct ccacggcatt gcaggaacaa
    10741 gctctctagc aagacttgtt gtatgtattc caagtcttat ggtacttata aaattctgct
    10801 tcgaccagtt tcctatcccg gtttccttat gaggtgttaa gtttgctgca aatcttattc
    10861 cccacggccc tgcccaggcg gtaagacttt tagaataaat acctgcccaa agtttatccc
    10921 ctatgagtcc ctcaggaata ttgggtaaat tttcatcact ttgtgaatgt cctgagagca
    10981 acctgttcgg gtcgggtata agaccaaccg ttgtttcaat ttttggagta tgacaggaaa
    11041 tacaaccata ttcgcttaca agatgcttag ccttttcgat atcacttgtt gttttgtcca
    11101 catcttgaga agttagcccg gttgaacatg aaagcaatat cgggatagat attattaaga
    11161 tgtaaaatat tttaatcata tatttaccat tataattgta atgctatcac agggcaagga
    11221 gaatgttcta caaccttttc tgcaacgctt ccaagatatt cacttcttat cttatctcca
    11281 ccataactca tcataactac caaatctata ttttcttcaa caatgaaatc tttaatcccc
    11341 acccatgaat tctgagcaac tctcactctt ggctccactc cactcatgtt tttagactta
    11401 agtaaatatt ccgataatgc agtataagaa ttgcccttta gagtctctat tacctctgca
    11461 ggataattat tgtcgccttt ttcaactata tttaacagat aaacctttga gttatatttc
    11521 tcggatattt cactggcata tcttaaatcc ttctttgcta tattatatat atcagttggt
    11581 acaagaactt ttttgaaagt gcttttttta aaatctgtat tgtgtacaaa gatactcaca
    11641 ttactgtctc ttaatatttt ttttgcagta cctcccaata tagattgacc cccaaacctg
    11701 ccttttccca tgactaccag gttataatta ttcttcttta tttcttttaa tatctcttta
    11761 tgaggaactc cgtaattaat ctcataacta aattttattc cctggttact gagttgttct
    11821 tttttcttct ccagcttttt tatatttttt ttctttaaat tatcttcaac ccagtggtca
    11881 aacaagttcc tttccgatac tgaaaacgat tttgtaaact cagtatgttc cggaagtata
    11941 ttcacagctc ttatttccga accaaatttc ctggcaatat tagcagaaac ctttagtacc
    12001 tgttcacttt cgtgtgttcc atcataaccg agcaatatct tgctgaacat ataatctcct
    12061 ataaacttgg tactgtttga taactatttt acatttatag agggaagaaa tcgatccccc
    12121 cctctaaact gaatccatga aaagcaacta tttaagacta atttttactt cctttggctt
    12181 tgaatcatct gcttttggca tatgaacctc cagcaagcca ttatcaaatt ttgcttctac
    12241 cttatttgtt tttatctttg atgggagatg aagcatcctg gaaaaacttc caaaagatct
    12301 ttcggaataa tagtaatcat cttccttgac ttcttcttct tttttcattt ccccttttat
    12361 tgttatactg tcttcagtag tagtaacatg tacatcttct tttttcatac ctggcatctc
    12421 cgcctttacc actatctcgt ccttattgtc atacatatca actacaggcg aaaactcttc
    12481 tatggactta atttttctca atgccggcat tctcatccag ccctgcggaa aatatggctc
    12541 ttcaaaaaac tcatcaaacc atttttccat attttttggt ttccatcttg ttagtgccat
    12601 gatttgcacc ccctaaactt tattttctat aaccatatta agtgcaactt taatgccaac
    12661 attgttattt taaaattgct gattaactta gcaaaaaacg ttatttaaat aattaatttc
    12721 cttttgaaat gtaagttgtt tggaaataag acagtaaatc ccccaagtga ttagagcaaa
    12781 tatataaatt ctatttcaaa acgctaaaga ttaaacatgg tacatctttt gcatttacta
    12841 ttattgatga aacagtatcc tagaacttat acaaccagac aaaatgtaaa actcactgtc
    12901 agaccaagaa ctgaagatga ctctgaatat ttaagtttat tttttttaga tataccaaaa
    12961 gaagatctta ttatttacag agaagatgtt tataaatcac attcaattga aacttggttt
    13021 cttgatgaaa gctataaaaa agatgtgcag ctcttagcat tatcggataa tgagatcatt
    13081 gcagaaggta caattcataa agaaggaata tactggcaaa atgctgccga gataaaactc
    13141 attgtcaaac ctgactacag aaaccaggga ataggttcgt atatgttcaa ttcactgcta
    13201 catgaatttt tagaaagaaa acttcaaaaa gtaatagtaa gatatacccc ggacaatatg
    13261 agttttataa aaatcattaa tcgttatgga ttcaaaccag aaactgtact caaatcatac
    13321 gtaaagatag ataaagacga taaagagcag gatcttataa tagcctctta taatctagta
    13381 gactggatca gaaagtttga gttttacaac ctcattactc caagacggta gtgtcttatt
    13441 atagttaacc tgcccgcata gtttttacta ttttaaacat ccagagcaat tcatatgtat
    13501 ctattcccag gcatttataa actaaaggta aaattgtttg tatcctattt aattaacaat
    13561 tgatttaatc tcattataat atctattcta tgctaaagat tatttctaca agagctaccg
    13621 acagagcagg taatggctaa aaaatcctca caaacacata acatctcaat attaaacaat
    13681 aaattaaagc gtgagatcga aaaacgcact cgtgctgaaa atgcattaaa gcacacattg
    13741 tctctgataa agaaaaaaac ctcgattaaa aaaataataa atactgtctt acaaagcgtt
    13801 cacaaatcgg tagatcctca aacagttatg aaaaatgctg taagcagtat gagcaggaat
    13861 atagagaacg cagataatgt ctctatatat atggttgaag caaaagaggc agtattaaaa
    13921 gctaatagag gttatccgaa gagttttatc aaaaaagtat ccgtaattcc gtatcctaaa
    13981 ggctttacct gggaaacttt aagaacagga aagctcatat actgtgatga taccgaaaaa
    14041 gataaattta tgggtcctgc gggcattgag atcggaacca aaagctatgc atccatgcct
    14101 attaaattag gcagaaaaac tattggcgtt ataaacatca attcacttaa aaaagaggcc
    14161 tttggaaaag aagacctgaa tatgctaaaa ctggttgctg agcagataca gacagcttta
    14221 aacaatgcca ggcatgcaga gcaactgcaa aaagaaaaag atacagccca gggatatctg
    14281 gatatatccg aagtaataat actggtttta aacgataaag gaaaagttga gcttataaat
    14341 aaaaaaggct gtaaaatcct cggatttaaa catgatgaaa taataggcag gaactggttt
    14401 gataatttcc tgccccaatc aatcaaatca gaagttaaaa gcgtttataa aaaagtttta
    14461 cagggaaagc tcaaaacagc aaaatattat gaaaatattg ttgtaacaaa gtctggcagg
    14521 gaaagaataa ttacatggca taacaccaga cttctaaaag atggaaaagc tattggaact
    14581 cttagctccg gagttgatat caccgattta agattagccg agcagcgatt cgaacaggtg
    14641 gtagaatcat cccctaatgg atttgtaatg gtaaatccta aaggcaggat agtgcttgtt
    14701 aatcgtgaga ctgaaaagct ctttggttat caccgcagtg aattaatggg acagacagta
    14761 gaaattttga tccccggtca agtgcgggca agtcacatgg attatcgtaa aaactttttc
    14821 ttaaatcctt caaacagggc tatgggagaa ggacgagacc tttttgccgt tcgtaaagat
    14881 gggacccggt ttccagttga aattggttta agtactctgc aaaccgaaag cggtgttgcg
    14941 gttttgagct ctgtagttga tataacagaa cgaaaaaacg ctgaaaaact agttaaggat
    15001 aatgagcaga agttaaaggc tattttagac agtactaccg atgcaatact ggtttatgat
    15061 aaaaacggaa gtataatgat aattaataac caggcgaaaa aaatgttttc tgaaaatgaa
    15121 aaaatcctgg agaacatatc acaaatcatt cctgcaagtc atagatcgat tcataatttc
    15181 cagctctctg aagccgaaaa tggaaccaac ctctttgatt atgagacaga aagagttcta
    15241 tcaaatggga aaaacattca tgcaagtatc gccctttcat atattcctgc acaaggcgga
    15301 atgtattttg aaagtattag agacattagt gaacgtgtta taatgagaca taaaattata
    15361 gactttgaaa aagcacaaat cattgcaagc atggccgaag gcattgcgca ccatatggga
    15421 acacccctgg cgtctatgct gttaagggta caaatgttaa aagaagatat agaacaattt
    15481 gacaacaatg attacttttc tcaaaagctg gaatctatag aaaaacagat ccattacggt
    15541 caaaaggtta tgcaaagact ccttagattt gcaagcaaac caacaagtga gagggtccct
    15601 tctaacatta agaatatagc tagtgatgct gtagaaatat taaagcccct gctgaacaaa
    15661 aaaagaatta aattagttat agatatcaat aaggattaca tgatacttgc tgatggtaat
    15721 atgattcagc tagtattttc tgaccttatc atcaattcga tccatgctat tgagaatgaa
    15781 ggcactatta aagtgactgc atctaatgat gaatcaaacg ataatttgac tattaaaatt
    15841 tcagataccg gaagtggaat tcctgaagag gtcataccct atgtgtttga gccatttttt
    15901 actaccaaaa ccactgagca ggggacgggt cttggtcttg cagtagctaa gagaatagta
    15961 catgaacacg atggagaaat tatgattgaa agcaaaaaaa atataggcac ggatattatt
    16021 atcaatctgg cgctttataa aggagatact aaatcttgag cacttttaaa gtacttatag
    16081 ttgatgatga ccaggaatat gtaaacacat taaaggagct tctcgaaaga aaccatatta
    16141 cagttattgc cgcttattct ggagaagaag ctttagagat gtttacaaac aatcccgata
    16201 taaggcttgc cctcgttgac cttattatgc ctatgatgga cggtattact cttatggaaa
    16261 aactaaaaga aaaaaatagt gaactgaata ttattatgat tacgggtcag ggcaccgtaa
    16321 caaatgctgt agaagctcta aaaaaaggcg ctaatgactt tattacaaaa cctttcgata
    16381 aagacatact tttaagcaag cttgagatat acagaaaatc atatgagctg gaaaacaagg
    16441 tcgaaaatct gtctagtata atatccacca aatatggttt tgataaaata gttagcagct
    16501 ccaaatcaat gaaaactgtg tttgaaaaag cagcagcagc agcaagaacc gatgctacgg
    16561 tttttttagt cggtgaaacc ggtacaggaa aagaacttct tgctaaatct atccacataa
    16621 agagcgacag aagccggtat ccgtttatcc ccataaattg tggcgcgatc ccaaaagagc
    16681 ttttggaatc agaactcttt ggacataaaa agggttcttt tacaggcgca gataaagata
    16741 atcagggtct ttttcttgca gcgcagcggg gcaccattct tttggatgaa atcggtgaaa
    16801 tgtcaaagga cctacaggta agactactga gagtattaca agagcaaaaa ataaggccta
    16861 taggcggatc aaacgaaatt ccaatcgaca ccagagttat tgcggcaagc aatcacagta
    16921 tagaagatct gaaaaataaa gaattgaggg aagacctgtt cttcagactt gccgtgattg
    16981 ttatagagat accaccactt agagatagaa aagaagacat acctattctt ataaatcact
    17041 ttattaatat attcaactct aaatatgata agaacattag cggagtaaat gaagatgtct
    17101 tgtccatact gtataattat gacttcccgg gaaacataag ggaacttgaa aatctttttc
    17161 agggtatagt agtgctagca ccggaggaca agaagtttat caatgaaaaa gacctgaaag
    17221 ccagtctggt ttggcatgat accaaaacat ctactaagtc tgttttatca cttgaaaaag
    17281 tagaaagatt tgcgattgaa aacgctataa gacaggctaa aggcaacaaa tcaaaagcag
    17341 cagaacttct aggtgtctca agggatacac tgtatagaaa actcaagcag tataacctcg
    17401 aagaatagta ttttattcta aatatattaa atacccctac atttcactct atgataaagc
    17461 tgtaagcatt tcttacacct tacaactagt ttaccaaaaa ccgcccaaat aacgcaatta
    17521 agtatggtat ggagattgca catattacta aaaacacggg cggtgataca atgataaaaa
    17581 aaatagtttt tccaacagac ttttcagaat actcatcaaa agccagagac tatgtagcag
    17641 aactcgcagg tactctgggc tccagagttt atataattca tgcaatcgag ccaattcagt
    17701 atgaagaaga tgatgaagag attaaacagt tctacagaaa cattgaatta gaaattaaca
    17761 ataagattga aaaggaaaag gactacttct tacaaaaaaa catagatgtt tacacagata
    17821 cggtaatcgg ttctgcatgg aaagtaatca acacatatgc aaaggaaaaa gaaattgacc
    17881 ttatcgtcat gggatcgcac ggttttaaaa ctgaaaaaga tgaagttgta ataggtacta
    17941 cgagccacaa agtggttctc acttcaccat gccctgtatt gatagtaaga aattaatcca
    18001 aggtaaaatt tatgaataga ctcttcatag acgacatgcc cgacagtttt tataaggaca
    18061 aaaaggttct ggtaagggta gattacaatg ttcctgcaat aagagggcaa attaaagaag
    18121 attacaggat cagacagaca attcctacca tagaatactt atctgaacgg ggagcaaaaa
    18181 caatattgat ctctcacctg ggaagaccag ggggcacccc ggataaaaag ctgtctctaa
    18241 aacctatagc cgaaagactt gttgaaattt taaaagtaag aaatgtaaaa tttgttgatc
    18301 aatgcattgg taatcatgtc aattatcttg cagatcattt acataagggt gaggtattgc
    18361 ttcttgaaaa cctaaggttt cttaatgatg aagaagataa tgatcccgag ttttgcagac
    18421 tgttttcgga tatagcagat atatatgtta atgatgcctt tagcacttca catagaaccc
    18481 acgcatctac ttacggaatt cccatgcttt tcgaacacaa actgggaggc tttgtactca
    18541 aaaacgaact atataattta gataaaatta aagaaaaccc aaaaagaccg tttcttgcaa
    18601 ttgtgggagg atctaaagga aaagaaaaga taggtgcaat aagcagtcta gtaaaatatg
    18661 cagacactat tctaataggt ggatgtgtag ctaatacttt tttggcagca agaggaatat
    18721 cagttggcga ctcaaatatt gcaaataaat ctatagaaca ggccaaaact atactgaggg
    18781 actatgcaga taaaattatc ctgcccgatg atttcctggt cgaagattgt gaccgtaggg
    18841 aaattttgaa aaatgttcat tatgaaatac ctcttggaca aaaagttgtt gatattggaa
    18901 gagatactac atactattat tcacatcaaa tattaaataa caatggaaca atattctgga
    18961 gtggcccgat gggacagttt gaagttgatg aatacgcgca tggcacaaac gaaattgcaa
    19021 agtctgtgtc ctatgcgcat tggagaggag catacactgt tgttggcggt ggggatacaa
    19081 ttgcatcgat ccgaaaagcc gatgtgctgc taaatgaaat tgactttgta tctaccggtg
    19141 gaagtgccac tttaaaatat ttagcaggga taaaccttcc tggaatatcg gttttatcag
    19201 a
//
LOCUS       DBNGSV010000277        17534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1019890, whole genome shotgun sequence.
ACCESSION   DBNGSV010000277 DBNGSV010000000
VERSION     DBNGSV010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17534
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1019890"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="AC159U_10250"
     CDS             complement(<1..389)
                     /locus_tag="AC159U_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337474.1"
                     /translation="MINKLKYPIFIGAAVFLCLIVSVFVLFNNNSRQILSSDNNINTS
                     RKVITQEDKPRIPERLVPLLLETTGRNIDDYKIIKDVDGTYKAQTKRNNLSATFNDHG
                     LTITPLYNKTSTWHIGLRFKGFGYENE"
     regulatory      complement(535..623)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(688..2616)
                     /locus_tag="AC159U_10255"
     CDS             complement(688..2616)
                     /locus_tag="AC159U_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3337475.1"
                     /translation="MYRFLGLLLVFLLSSDAFTQQFDANMAWPLCGRITENPPAGWQV
                     GDDCPAERFGNPDHTDLPIRSVFGPRPLVSENERYDFHRGIDIATPIGTPVFAVADGE
                     VRTAGDHPSYSDPVIIIRHFRNGETSCSNSGCYHSNYLHMDSAVVNQGQNVSKGQFIG
                     YSGESGSGFDHLHMEIRDAKPEDPFSSWQRDCINPVGILPYVSTDSATITFNSTIADL
                     SQPFVQITVESPRVDINRVELFLYDQSDNLVQQPGNTADSLGYNVNPSWFDVNLWNRQ
                     YTHKDSSSVPWESFDSGGVNECPYHADHPNSYDPHIHMDAADPNDYQVGLFNGIRIQT
                     QKFHISGYYINLAFNELTGPSAQQAPVKCVVATVTFGAGGDATNTLGDCSGVLDPTPD
                     ECGNGTCESSETCSSCPSDCGSCSSCGDGTCDGDETCTTCETDCGTCSTCGNGACDIG
                     EDCNSCPSDCVSGSGPSCGNGICEAAAGEDCNSCASDCDSKTKGNPKTHYCCGDGGVC
                     GSSQCNADPYSCTETTSASYCCGDAVCEGAEDSSSCELDCGSPPVCGDGTCDTGEDIC
                     SCSADCGTPPASELSCSDGGDDDCDGAIDCSDSDCDSDPACSSSCTPTHSNEKGKRCS
                     DGLDNDCDGFTDGADPDC"
     gene            complement(2852..3949)
                     /locus_tag="AC159U_10260"
     CDS             complement(2852..3949)
                     /locus_tag="AC159U_10260"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR3337476.1"
                     /translation="MSGTNSSELEGNRKIRFLPVREFEKELRARVDEYFNDKNLTKRD
                     NLRMYGKTAIVLSWLIASYVFLVFMNLPTWGIVLSAVSLSLALNAVGFNIMHDGNHRA
                     YSNNPYINKLMGYSLDLIGGSSYFWNWKHNYLHHTYPNITGIDDDIEPGIFARLSPHQ
                     KRYFFHRYQHIYIWIIYGFLIINWHVYQDFYNYITKKIHNHTFPRPKGWDLFMFFFGK
                     ILFFSLALVIPSFFYPFWAVALAYFLIVFAQGIVLTIVFQLAHCNEEADFVEHENDDE
                     RLGSSWLVHQLKTTADFSRDNKILNWYVGGLNFQVEHHLFPKICHVHYPELSKIVEKT
                     CNEFGITYNAYKNLSEGIASHYRWIYQMGRA"
     gene            complement(4088..4618)
                     /locus_tag="AC159U_10265"
     CDS             complement(4088..4618)
                     /locus_tag="AC159U_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337477.1"
                     /translation="MMNISSHIRSSDLDPGVAYDYKTQQVIDQIFSSFADYLIGIKEI
                     ANLMIYIVDEENNELRLVVHRNMHQDFPRNFSRIEKTKYLARNIIKSNSVLESQDTNF
                     RMISNKFGMNIQKGFIGFPIKDNVDSLSKPKGAILVWTSYINKFDQNKYNFIVSAAKY
                     LSKSIKWNNLFAEEHN"
     gene            4802..6085
                     /locus_tag="AC159U_10270"
     CDS             4802..6085
                     /locus_tag="AC159U_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3337478.1"
                     /translation="MWEQIRKNKRNSVILVLCMAVVLFILGYFLGEYLYPGYGIYGLY
                     AAFGLWSVQSTVSFFAGDKIVLGISKARKINRDMHPTLYNVVEEMKIASSLPKVPDVY
                     IIDDPALNAFATGRKPETSAVAVTSGLLEVLTRDELQGVIAHEVAHIKNRDVLFMTMV
                     GIMMGTIVLLADLGLRHMFWGGGGRRRTSRDEGGQIQAVIMIIAVIFIILSPVIAQLI
                     YFAISRKREYLADAGAVQFTRYPLGLAGALEKIGSSTDQLKSATKATAPMYIANPFKG
                     KKQSLKNLSSTHPPLDERIKILRNMTGAGLGDYDNAYKKTTGKPVGTIPKSALETEKS
                     SPLGTVFPEMGLVAGAAIQKEATMTPDAKIQITTDKAEAHINRTRETTDALWKLNEYK
                     FIECECDTNLKIPEAYSNKVIECPHCLKNHRVAQL"
     gene            complement(6131..6691)
                     /locus_tag="AC159U_10275"
     CDS             complement(6131..6691)
                     /locus_tag="AC159U_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3337479.1"
                     /translation="MIQHAEVNQEEVMRNLNKLASSTNETENLFETSMNYLTDFYNID
                     VVMIYLLDKDTGKAHLTGKKNVPPRYVESSSEMDESSGKLWEVIKENKIINIKNVQED
                     ESFGFDLRSLNKKGALGIPISLSYTGDAQGALWIFSDDEYNYDPQKDKTLLYLVNQIA
                     TSIAWAMKYEEKRGNWQERFKISLNS"
     gene            7221..7808
                     /gene="hisB"
                     /locus_tag="AC159U_10280"
     CDS             7221..7808
                     /gene="hisB"
                     /locus_tag="AC159U_10280"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3337480.1"
                     /translation="MPRKAKVKRKTSEVDVSIDINLDGSGNYQISTGVAFFDHMLEQF
                     SKHGFFDLTLKAKGDIEIDFHHTVEDVGITLGDAIQKALGDKKGIKRFGHAYVPFDET
                     LVFAAVDLSGRPFCVYNMQTPKSKVGEFDTELAEEFFKSFTNSLKCNLHIELKYGDNL
                     HHIIEATFKAVARALDTATSIDPRSNKIPSTKGML"
     gene            7812..8453
                     /gene="hisH"
                     /locus_tag="AC159U_10285"
     CDS             7812..8453
                     /gene="hisH"
                     /locus_tag="AC159U_10285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3337481.1"
                     /translation="MIAIVDYGMGNLRSVSKAFESQGFSSKITNDSDTIMSSNGLVLP
                     GVGAFGDCIKNLKEYKLFDLIPEYIQTGKPYLGICLGYQILFESSEESPDQKGLGFIK
                     GKVIKFDFEKELNLKIPHMGWNQINIKKQSEILDGIDDKSWFYFVHSYYPVPADSNIV
                     AITADYGGEFTAAIQKDNVFACQFHPEKSSDLGLKIVRNFARKCGLRLEDKAV"
     gene            complement(8450..10462)
                     /locus_tag="AC159U_10290"
     CDS             complement(8450..10462)
                     /locus_tag="AC159U_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3337482.1"
                     /translation="MKKLLKIIFGFFVFIAAFNTDLRASVPIDCEVVLGEKERSNEAN
                     NIYRRAYCNVKQENYSEAIELFENVSDKLPVLSDHNIYYRAFSYHKLKNNDMALKLYH
                     ELASKYPNSTLKKRVSESLAEIYFQQKEYAKARELYQDLYSAEIKDKKKPEYLFGVAN
                     SLKGQNKLQEAINTYKSLWIQYPTSKYSKLSNELALNIAKDNNLLFKISPNDYYSRAQ
                     KFFISSNWNSALKNYKKSPQTLAVKVNTAISYLRIGHYTRALEILGKINSSKSLFWQA
                     QIRAKQDMNELAANIHLQLVKSHPNSHFAPQALFNAAKLYEIDKKLDDAIKVYEQLIK
                     KYPKTEYAEEASWNLGWIYYKQNEYLKAHNTFTGFINSGSSFTSTRSEYWSARTLEKL
                     GNDKQANYIYKRLAGRFFPSYYPYLAQIKTGYDDKSKIKNETLDTSKSDSKRIAKALL
                     LIELGILDDALLEVKYLHNAAKSVSDFIDLSILYSKANDFYNAIQVVQGINHPDALRL
                     SYPQGYSEIVKKYSKLYGVDENIVYSIMREESRYQKDVVSPAKAIGLMQLIPATGRTT
                     AKELGIKNYSKAKLTDPETNIRLGSYYFKKVLDEFGGNVFFALGGYNAGPHRIYEWQK
                     RFPGLNMDEFVEEIPFRETRNYVRRVLRTYGAYKAIYAGDIDTEMN"
     gene            complement(10498..11307)
                     /locus_tag="AC159U_10295"
     CDS             complement(10498..11307)
                     /locus_tag="AC159U_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3337483.1"
                     /translation="MLLKIQLIVSSAVIAAIVCIAIFAPAISPYKYDKTNFSNILQSP
                     SKAHPMGTDQEGRDLLSRVIYGSRVSMIVAIGTALLALFIGTAYGGLSGYLGGKIDEL
                     LMRIVDVFYSLPDLLLIVLVMLILGQSLFGIILALSVTGWMRVARVVRGSVLQVKNYD
                     FIESARSLGASPLRVFFRHIFPNILSPLIITLTFSVPYAILAESTLSFLGLGIRPPES
                     SWGTLASTGWQGMRTFPHLIAFPSIAIFVTAFSFNMLGESLRDYFDPKSVV"
     gene            complement(11338..11688)
                     /gene="queF"
                     /locus_tag="AC159U_10300"
     CDS             complement(11338..11688)
                     /gene="queF"
                     /locus_tag="AC159U_10300"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR3337484.1"
                     /translation="MDKKILETFENEYQGREYEIDITCPEFTALCPKTGQPDFAVINI
                     KYVPDKLCIELKSLKLYMFAYRNEGEFHEHVTNKILDDVVSVCKPIRATVTGDFNVRG
                     GIKTIVRASYNKKD"
     gene            11828..11901
                     /locus_tag="AC159U_10305"
     tRNA            11828..11901
                     /locus_tag="AC159U_10305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11860..11862,aa:Gln,seq:ctg)
     gene            complement(11887..12810)
                     /locus_tag="AC159U_10310"
     CDS             complement(11887..12810)
                     /locus_tag="AC159U_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR3337485.1"
                     /translation="MFVEYSTGAYSFGELAKKAKEWGLRNKENTKTYLSTSQIHHMIK
                     NPFYYGEMLSVGKLYKHKYPPIIDKFTWDKCQQIRTGRSNSKAPRQTKKPFIFRGLLT
                     CAISGKAVTSDLKKGKYVYLICRDPNNPGKKMFVPEKIVLDQIKDVFQSIRIEKDMLL
                     SVTEHLKVMHDSEKDHQARAITELEKENKDIQKKLDRLLDLLIDESITSDDHERKCQE
                     LKNRQYDINEQIKKYLKADESFQVTVKTVLSLASKAYEIFESSNIEQKRKLINYVFSN
                     LQLRGVTLEYHLKKPFDLMVDCTTYSEWLGH"
     gene            complement(12829..13368)
                     /locus_tag="AC159U_10315"
     CDS             complement(12829..13368)
                     /locus_tag="AC159U_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR3337486.1"
                     /translation="MKAIILARVSSKEQQDGYSIQTQTEQLTDYCNRKNLDVIKTYQI
                     VESSTRGDRKEFRAMLDYISRQHSTIALVVTHIDRLQRSFKEYPLLDNYINDEIIEIH
                     FINENKIIHKHSSASDKLVWNIGIAVAQNQVDILSEKVRSALHTKRENGEWGGKAPIG
                     YTNVSDNDSGRNSIVIDKD"
     gene            complement(13391..16522)
                     /locus_tag="AC159U_10320"
                     /pseudo
     CDS             complement(13391..16522)
                     /locus_tag="AC159U_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
     gene            complement(16525..>17534)
                     /locus_tag="AC159U_10325"
     CDS             complement(16525..>17534)
                     /locus_tag="AC159U_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337487.1"
                     /translation="DDKKRHGYLPRLTLFMRNKPNGFFVQLKIEFSAPKLLFGNNFDE
                     LEDNDFDRLIYKLKDQLLIMGVVTTIDALKNAKVSSIHYSKNIILDKHLNSAMILSEL
                     NKVNISKRLDLSTTDFRNGGHALRYHTNSYQIVFYDKIKDLEKAKVSDKRAIERDNDM
                     QLDLFKKYKETNNPDVIRMEIRLGNKRTIENILKKTGINSGITSNNLFNSTISKKILL
                     YHFDAIFDEWFIGVDKQIKPEDYFIQINKAANYKNSKILQLIGAIYIIESIGSKGLRN
                     LLDNQSDRSWYNLKSDLDNIDFKSVNKTDMFKKVYADLNKFNKLEMSDVFQSNKHKVL
                     N"
BASE COUNT         5174 a         3505 c         3072 g         5783 t
ORIGIN      
        1 atttcatttt catagccgaa acccttaaat ctaagtccaa tatgccatgt tgaagtttta
       61 ttgtataaag gagtgatagt gagtccatga tcattaaaag ttgctgacag attattcctc
      121 ttagtctgtg ctttataagt tccgtctaca tcttttatga tcttataatc atcaatgttc
      181 cttccagtag tttccagaag caatggaacc agcctttcgg gaattctggg tttatcctct
      241 tgtgtgatta ctttccggga tgtattaata ttgttatctg agcttagaat ctgccttgaa
      301 ttattgttga aaagtacaaa tactgaaaca attaaacaaa gaaatactgc agctcctata
      361 aatataggat atttaagttt gttaatcatt ttccccgctc ctttattaaa gaattatgat
      421 taattaaaag tttattgttt tattacatat aagtcttcaa ccccacaaga taaaactctt
      481 ctgcaaagat tattgggact tatgggatca ggaaaaagag aagtgatacc cgatgtcagc
      541 agcctggcag cctgatttct ctcgaaactt acggcccaat agctttgcgt cccacccttt
      601 caagtggttt gccttttctt atcgtacatt tagatattaa tttttattaa taaagaagtc
      661 aataaaaata tttttattta aattgtatta gcagtccgga tcggccccgt ctgtaaatcc
      721 atcacagtca ttgtctaacc cgtctgaaca tcttttaccc ttttcatttg aatgagtcgg
      781 cgtacaagaa ctactgcatg caggatccga atcacagtca ctgtcactgc aatctatagc
      841 accgtcacaa tcatcatcac cgccatcaga gcatgacaat tcagaagctg gtggtgtacc
      901 acagtctgca gagcatgagc aaatgtcttc tcctgtatca caagtgccgt caccacatac
      961 cggtggcgat ccgcagtcaa gctcacagct gctgctgtct tctgcaccct cacatactgc
     1021 atccccacag cagtaagacg ccgaagttgt ttcagtacat gagtatggat cggcattaca
     1081 ctggctgctg ccgcatactc caccatcacc acagcaatag tgagtctttg gattgccctt
     1141 ggtttttgag tcgcagtcag aagcacagga attacagtct tctcctgccg ctgcctcaca
     1201 tattccattg ccgcatgaag gtccgcttcc ggaaacacaa tcggatgggc atgaattaca
     1261 gtcctcccct atatcacaag ctccattacc acatgtactg catgtaccgc agtcagtctc
     1321 acatgtagta caggtttcat ccccgtcaca tgtgccgtct ccacagcttg agcatgaacc
     1381 gcaatcagaa gggcatgaag aacatgtctc tgaagattca caagtaccgt ttccacattc
     1441 atctggtgtc gggtctaata caccagaaca gtctccaaga gtatttgttg catctcctcc
     1501 tgcaccaaat gttacagttg ctacgacaca ttttacaggt gcttgttgtg cgctgggtcc
     1561 agtaagttca ttgaatgcaa ggttaatata atagccgctt atatgaaatt tttgtgtctg
     1621 tattctgata ccgttaaaaa gaccaacctg gtagtcgtta ggatcagccg cgtccatatg
     1681 aatatgcgga tcataagagt ttggatgatc tgcatgataa ggacattcgt ttaccccccc
     1741 actatcaaaa ctctcccaag gaacgctcga gctgtcttta tgagtgtact gcctgttcca
     1801 tagatttaca tcaaaccatg aggggtttac attataacca agagaatctg cagtatttcc
     1861 cggttgctga accaaattgt cagattggtc atataaaaat agttctactc tgtttatatc
     1921 aaccctgggt gattctacag tgatctgaac aaacggttga gaaagatctg caatggtact
     1981 attaaaagta atagttgccg aatctgtact cacatacggc aagattccta cagggttaat
     2041 gcagtctctt tgccagcttg aaaacgggtc ttcgggctta gcatccctta tttccatatg
     2101 gaggtgatca aaaccgcttc cactctcacc ggaataacca ataaactgtc ctttgcttac
     2161 attttgtccc tggttaacca cagcgctgtc catatgaagg tagttgctgt gataacatcc
     2221 agaattgctg catgaggtct cgccgtttct aaaatgccta atgattatta caggatcaga
     2281 atagcttgga tggtcacccg ccgttctaac ttcaccgtcg gcaactgcaa ataccggtgt
     2341 tcctataggg gtagctatat caatccctct gtgaaaatcg tatctctcat tttctgatac
     2401 aagtggcctc ggcccgaata ccgatcttat cggtaaatct gtatgatcgg gatttccaaa
     2461 cctttcggca gggcagtcgt ctccaacctg ccatcccgca ggaggatttt cagtaattct
     2521 tccgcaaagc ggccaggcca tattcgcatc aaactgctga gtgaaagcat ctgaagataa
     2581 caaaaatact aaaagtaatc ctaaaaatcg atacataata ccaatcctcc attaagctac
     2641 tgactaattt acgattaatt ataacctctg tactatttaa taatcaagtc ttaaatatta
     2701 caaaagtcta attcaaaggg atcgataaaa caggaactga cgtaaagaat atattatccc
     2761 atacaaatca tcttggctca tcaagatatt taacagactt agaaatagaa gatgtatgga
     2821 ctgaacatca tacaaaatct atacggttat tttatgctct tcccatctga taaatccagc
     2881 ggtaatgcga tgcaatacct tcagagaggt ttttatatgc attgtatgta atgccaaatt
     2941 cattgcatgt cttctcgaca attttggaaa gttcagggta gtgtacatga caaatctttg
     3001 ggaacaggtg atgctctacc tgaaagttaa gcccgcctac ataccagtta agaattttat
     3061 tatctctcga gaagtctgct gtggttttta actgatgcac aagccagctg gaaccgagtc
     3121 tctcatcgtc attttcatgc tctacaaaat ccgcttcttc gttacagtga gcaagctgga
     3181 acactatagt aagaacaatg ccctgggcaa atacaatcag aaagtatgca agtgctactg
     3241 cccaaaatgg ataaaagaac gatgggatta caagagctaa agaaaaaaac aagattttac
     3301 caaagaaaaa cataaacaga tcccagccct ttggacgcgg aaaagtatgg ttatgtatct
     3361 tcttggttat gtagttatag aaatcctgat aaacatgcca gttgattatg agaaaaccgt
     3421 aaataatcca tatatagatg tgttggtatc tgtggaaaaa atatcttttt tgatgagggc
     3481 tcagcctcgc aaatatgcct ggttctatat catcatctat gcctgtgata tttggatatg
     3541 tgtggtgcag gtaattgtgt ttccagttcc aaaaatatga gctgcctcca ataaggtcga
     3601 ggctatagcc catgagtttg ttaatataag ggttattgga atatgctcta tggttaccat
     3661 catgcatgat attaaaacca acagcattta atgccagact caaagaaacg gctgagagca
     3721 caatacccca tgttggaagg ttcataaaaa ccaaaaaaac atatgaagct atgagccagg
     3781 aaagtacaat agctgttttg ccgtacatcc tgaggttgtc tcttttagtt agatttttat
     3841 cgttaaagta ttcgtccact cttgccctaa gctctttttc aaactctctt acaggcaaaa
     3901 atctgatctt tctattacct tcaagttcac tactgtttgt accactcatt gccttacatc
     3961 ctgcattaaa ttatttaacc gccaatttta tgccctaaaa ggatatattt caactattat
     4021 ttaaatttaa taaataaata ttgaaatgta gaaaagaaaa tcgggactca taggagcact
     4081 attaatatta gttatgctcc tcggcaaata aattattcca cttaatagat ttacttaaat
     4141 atttagctgc agacactata aaattatatt tattttgatc aaacttgttt atataagaag
     4201 tccaaacaag aattgcaccc tttggtttag acaggctatc cacattatcc tttatgggaa
     4261 atccaataaa tcctttttgt atattcatac caaatttgtt cgatatcatt ctaaaatttg
     4321 tatcttgtga ttctaataca ctattggatt taataatgtt tcttgccaga tattttgtct
     4381 tttcaattct tgagaaattc ctcgggaaat cctgatgcat atttctgtga actactaatc
     4441 ttaattcatt gttttcttca tccactatat aaatcataag attggcaatt tcttttatgc
     4501 ctataagata atcagcaaaa ctgctaaata tctggtcaat tacctgctga gttttataat
     4561 cataagcaac acccggatct aaatcactgc ttctaatatg agaagatata ttcatcattg
     4621 cctccataat ttcaattaca atgaattctt gtatttattc ctgtatttat taattgcaaa
     4681 cttcatacca attaattatt tattcaaaga ggtttaaaag ttagaataat cgagattaat
     4741 gcttataatt aatatcatat ataactatta taattattat tgtaattaaa cggatctaag
     4801 catgtgggaa caaataagaa aaaataaaag aaactctgtc atcttggttt tgtgcatggc
     4861 tgttgttttg tttattttag gatatttcct tggtgaatat ctgtacccgg gatacggtat
     4921 ttatggactt tatgcagcat ttggcctatg gtctgttcag tctactgtta gtttttttgc
     4981 cggtgataag attgtgcttg gcataagcaa ggcaagaaaa attaacaggg acatgcatcc
     5041 aacactttat aatgttgtag aagagatgaa aatagcatca agccttccaa aggttcccga
     5101 tgtttatatt atcgatgatc ctgccctaaa cgcctttgca acaggcagaa aacccgagac
     5161 atctgcagtc gctgtaactt cggggctttt agaagtttta acaagagacg agcttcaggg
     5221 tgttatcgct catgaagtgg cccatataaa aaaccgggat gttcttttta tgaccatggt
     5281 tgggataatg atgggaacta ttgttcttct tgctgatctc ggactaaggc atatgttttg
     5341 gggcggaggg ggacgcagga gaacttcaag agacgaaggt ggacaaattc aggcagtaat
     5401 aatgattatt gcagtaattt ttataatact ctcaccagtt attgcccaac ttatatactt
     5461 tgcaatttca agaaaaaggg aatatcttgc cgatgcagga gctgttcagt ttacccggta
     5521 cccacttggt ctagcaggag ctctcgaaaa aatcggcagc agcactgacc agctaaaatc
     5581 tgcaaccaag gctacggctc caatgtatat agcaaaccca ttcaaaggaa agaagcaaag
     5641 tcttaaaaac ctcagcagta cacatcctcc gcttgatgag agaataaaaa tattaagaaa
     5701 catgacagga gcaggactcg gtgattatga caatgcttat aagaaaacaa ctggaaaacc
     5761 tgtaggaaca atccccaaat ctgcgctgga aactgaaaaa tcgtcaccac ttggaactgt
     5821 ttttcctgaa atgggccttg tggccggagc agcgattcaa aaagaagcaa ctatgacccc
     5881 tgatgctaaa atacaaatta caacagataa agcagaggca catattaacc gcacaagaga
     5941 aactactgat gctctatgga agttaaatga atacaaattt atagagtgtg aatgtgatac
     6001 aaacctaaaa ataccagaag cttattcaaa taaggtaatt gaatgccctc attgtttaaa
     6061 aaatcataga gtagctcaat tatgagctac tccatgaatt atacttaata acaagaagga
     6121 tttaagaatt ttaagaattt aggctgattt taaaacgttc ctgccagtta cctctctttt
     6181 cttcatattt catagcccat gctatggatg tggctatctg atttacaaga tatagtagtg
     6241 tcttatcttt ttgaggatca tagttatatt catcatcact gaaaatccat aaagcccctt
     6301 gagcatcgcc agtgtagctt agagaaatag gaatacctaa tgcacctttt ttgtttagtg
     6361 atcttaaatc aaatccaaat gattcatctt cctgtacatt tttaatattt ataatcttat
     6421 tctcttttat tacttcccat aatttaccgc tcgattcatc catttccgat gaactctcta
     6481 cataccttgg aggtacattt ttctttccgg ttagatgagc cttccctgta tctttatcaa
     6541 gtaaataaat catcacgaca tcaatattgt aaaaatccgt cagataattc attgatgtct
     6601 caaacagatt ctctgtttcg tttgtagaac tcgcaagctt gtttaagttc ctcataactt
     6661 cttcctgatt tacttcagca tgttggatca tttcttaatc cctcctgttt gctatttgaa
     6721 atagtaaatt gcaaatataa tgccaacttt atatttgtac ccatttatgc tatatgcaac
     6781 ttactcaaaa acataattat ctaatcattt aaatgagtta tgacatttat caattaacta
     6841 aactatttaa ccacgataaa ttaatatata tttacttcaa acccaacgat cataaaatat
     6901 aaagacggga ataaattccc ttaattattc taagtcttta atcgctctaa tatagacatt
     6961 ccaaaataat tgttttaata ttaattgtat gggtcaatta actgaattat tctcaaattt
     7021 atctaattct ttagacgata ttcccaatta ctactatcta ggcgctgcgg tgctttttgc
     7081 cctcattttt tatttcgcag taaaaaggta attgcggcat ttatagtact atttaataaa
     7141 catattataa aaaccagtgt ggtagagttt aaatactcct gcatgttgga tttcagttct
     7201 ttttcagata agattcttcc atgccaagaa aagcaaaagt aaaaagaaag acttcagaag
     7261 tggatgtttc tatcgacatt aatctagatg gaagtggaaa ttaccagatt tctactggag
     7321 tagcattttt tgatcacatg ctcgaacagt tttccaaaca tgggtttttt gacttaacac
     7381 taaaagcgaa aggtgatatt gaaatagact tccatcatac agtggaagat gttgggataa
     7441 cgcttggcga tgctatacaa aaagcgctag gagacaaaaa aggtataaaa agatttggtc
     7501 atgcatatgt accttttgac gaaaccctgg tatttgcagc tgttgatctg agtggacgtc
     7561 ctttttgcgt ttacaatatg cagaccccaa aaagcaaagt tggtgaattt gacactgaac
     7621 ttgccgagga gttctttaaa tcttttacaa actctttaaa atgcaatctg cacattgaac
     7681 ttaaatatgg tgataacctg caccatataa tagaggctac ttttaaagca gttgcaaggg
     7741 cgcttgatac agctacctct atagacccgc gctcaaacaa aataccttca acaaaaggga
     7801 tgctctaggc tatgattgcc attgtagact acggcatggg taaccttagg agtgtaagta
     7861 aagcttttga aagtcagggc ttttcatcca agattactaa tgactctgat accattatga
     7921 gttcaaacgg tcttgtactc cctggtgtag gtgcatttgg agactgtatt aaaaatctaa
     7981 aagaatataa actcttcgac ttaattccgg agtacataca aaccggaaaa ccatacttgg
     8041 gtatttgcct tggctaccag atactttttg aaagcagcga agaatcccct gatcaaaaag
     8101 ggcttgggtt tataaaagga aaggtaatca agtttgattt tgaaaaagag ttaaacctca
     8161 aaattcctca catgggttgg aatcagatta atataaagaa acaatctgag atattagatg
     8221 gaattgatga taaaagctgg ttttattttg tgcactcata ctatcctgtt cctgctgata
     8281 gcaacatagt cgcaattaca gcagattatg gaggcgaatt tacagcagcc attcaaaagg
     8341 ataacgtgtt tgcttgtcag ttccacccgg aaaaaagtag tgatctgggg cttaagatag
     8401 ttagaaattt tgccagaaag tgtggtctga gattagaaga taaagctgtt taattcattt
     8461 ctgtatcaat atctccggca tatatagctt tataagctcc gtaagttctt aacactcgcc
     8521 tcacataatt tctcgtttcc ctaaatggaa tttcctctac aaactcatcc atattaagac
     8581 cgggaaatct tttttgccat tcatagatgc ggtgaggccc agcgttataa ccacccaggg
     8641 caaaaaatac attgccgcca aattcatcca atactttctt aaaataataa gagcccagcc
     8701 ttatgtttgt ttcaggatct gtaagcttag ctttactata gtttttgatc ccaagctcct
     8761 ttgcggttgt tcttccggta gcaggaatga gctgcataag accaattgct tttgctggag
     8821 atactacatc tttttgatac ctgctctcct ctctcattat ggagtaaaca atgttttcat
     8881 ccactccgta gagctttgaa tatttcttta caatttcact atacccctga gggtatgaaa
     8941 gcctcagagc atccggatgg ttaataccct gaactacttg aatggcatta taaaaatcat
     9001 ttgctttact atatagaata ctaaggtcga taaaatcact gactgatttt gcagcattat
     9061 gaagatattt cacttcaaga agcgcatcat ctaatatgcc aagttcaata agcagtagcg
     9121 cctttgctat tctcttcgaa tcagattttg atgtatccag tgtttcattt ttaatttttg
     9181 atttatcatc ataaccggtt tttatctgag cgagataggg gtaatatgat gggaaaaatc
     9241 ttccggcaag acgtttataa atataattag cctgtttgtc atttcccagt ttttctaacg
     9301 tacgcgcgct ccagtattcc gatctcgtag aagtaaaaga cgagcctgag ttaatgaatc
     9361 ccgtaaatgt attgtgtgcc tttaaatatt cattttgttt ataatatatc catcccaggt
     9421 tccatgacgc ttcttctgca tattctgttt taggatattt ttttattaac tgctcataca
     9481 cttttattgc atcatcaagt tttttatcta tctcgtagag ctttgcagcg ttaaatagtg
     9541 cctgaggagc aaagtgactg tttggatggc tttttacaag ctgcagatgt atatttgcag
     9601 caagctcatt catatcctgc ttagcccgga tttgagcctg ccagaacagc gacttggatg
     9661 aatttatttt acccagtatc tcaagtgctc tagtataatg cccgatcctc aaataactga
     9721 tggcagtgtt taccttaaca gctaaagtct gcggagattt tttatagttt ttaagagctg
     9781 aattccagtt tgaagagatg aaaaattttt gagccctgct gtaataatca tttggagaaa
     9841 ttttaaatag aagattgtta tctttagcaa tgttaagagc cagctcattt gaaagtttag
     9901 aatatttaga ggttggatac tgtatccaca ggctcttgta agtgtttata gcctcctgta
     9961 atttattctg cccctttagc gaatttgcaa caccgaataa atactccggt tttttcttat
    10021 cttttatctc tgcactataa agatcctggt aaagctccct ggcttttgca tattcttttt
    10081 gctgaaaata aatctcggca agactctcag aaactcgttt tttaagagta ctatttggat
    10141 atttacttgc aagctcatga tacagcttta aagccatatc attatttttt agcttatgat
    10201 aggaaaaagc tctgtaataa atattatgat cagataaaac tggaagctta tccgatacat
    10261 tttcaaaaag ctcgatggcc tcagagtaat tttcttgttt tacattacaa taggcacgtc
    10321 tgtatatatt gtttgcttca ttagacctct ctttttcacc aagtaccacc tcacaatcaa
    10381 taggtaccga cgcccttaga tcagtgttaa aagctgcaat aaaaacaaaa aatccaaaaa
    10441 taatttttag tagttttttc atagaaagaa taaatttcat catattagtt tagttaatta
    10501 aactaccgac ttaggatcaa aatagtctct aaggctctct cccagcatat taaacgaaaa
    10561 tgctgttaca aatatagcga tgcttggaaa tgcgattaag tgtggaaatg ttctcatccc
    10621 ctgccagccg gtgcttgcaa gagtacccca gctcgactct ggcggcctta ttccaagccc
    10681 caaaaaactt agagtggatt ctgccaatat tgcataaggc acagaaaaag taagggtgat
    10741 tatcagcggg cttaagatat ttgggaatat gtgtctgaaa aatactctaa gcggtgaagc
    10801 ccccaggctt cttgcagatt ctataaagtc atagtttttc acctgtaaaa cactccctct
    10861 tacaactctt gcaaccctca tccatcccgt aacacttaga gcaagaataa ttccaaaaag
    10921 gctctgaccc aagataagca ttaccagtac tatcagcaac aaatccggaa gggaatagaa
    10981 tacgtcaaca attctcatta gaagttcatc tatcttccca cccaaatacc ccgatagccc
    11041 tccatatgcg gttccaatga aaagcgccag aagagcagtc cctatggcta caatcataga
    11101 taccctcgag ccatatataa ccctgcttag aaggtccctt ccttcctgat ccgtgcccat
    11161 agggtgtgct tttgatggag attgcagaat attggaaaaa tttgtcttat catatttgta
    11221 cggagaaata gcgggcgcaa agattgcgat gcaaacaatt gctgctatta cagctgagct
    11281 tactattaat tgtattttta gaagcaaggt taaacccaag attaaattaa aattatatta
    11341 atctttttta ttgtacgagg ccctaacgat agttttgatt cctcccctta cgttaaagtc
    11401 tcccgtgact gttgctctaa taggtttaca tactgaaact acatcatcaa gtattttatt
    11461 tgttacatgc tcatgaaact caccctcgtt tctgtaggca aacatataaa gcttaaggga
    11521 tttaagctca atgcaaagtt tatccggtac atatttaatg tttataacag caaaatcagg
    11581 ctggcctgtt tttggacaga gtgcggtaaa ttcagggcat gtaatatcaa tctcatactc
    11641 ccttccctga tattcattct caaatgtttc taatattttt ttatccattt tgttttcaga
    11701 ttaataaaat tacgaattaa tggcaatgct ccacgcacaa atattgccat tttactttaa
    11761 attttcaaac aattgggtgt aagtcttgac tgatgggcaa ttttataaaa taataacagt
    11821 cttttcctgg ggcatagtac aatggcagtg cggcggactc tggctccgca aatctaggtt
    11881 cgaatcctag tgccccagcc attcagaata ggtagtgcaa tctaccatta aatcgaatgg
    11941 ttttttcaaa tgatattcga gggttacgcc tcttaactgc aagttcgaaa atacatagtt
    12001 tataagtttg cgtttttgct ctatattcga actttcaaat atttcatatg ccttggatgc
    12061 tagagaaagt acagtcttta cagtaacttg gaaagattca tcagctttga gatatttttt
    12121 tatttgttca ttaatatcgt actgcctatt tttgagctct tgacattttc tttcatggtc
    12181 atccgaagta atactctcat ctattaatag gtctagtagc ctatctaatt tcttttgtat
    12241 atctttattt tctttttcta gttcagttat agcccttgcc tgatgatctt tttctgaatc
    12301 gtgcataact ttcaaatgtt cagtaactga taaaagcata tctttttcaa ttcttataga
    12361 ctgaaacaca tctttaattt gatctagcac tattttttct ggaacgaaca tttttttacc
    12421 cggattattc gggtctctac aaattagata tacatattta cctttcttta aatcagaagt
    12481 aacagctttt ccagaaatag cacatgttaa caatcctctg aaaatgaatg gttttttagt
    12541 ctgtctagga gcttttgaat ttgaacgccc tgttctaatt tgctgacatt tatcccaagt
    12601 aaatttatca attattggtg gatatttatg tttatatagt tttccaactg acaacatttc
    12661 cccatagtaa aatgggttct taatcatatg atgtatttga cttgttgata agtaagtttt
    12721 tgtattttct ttatttctaa gaccccactc tttagctttt tttgctaatt ctccaaatga
    12781 atatgcacca gttgaatatt caacaaacaa tttcttaact acaaatgctc agtctttatc
    12841 aataacaatt gaatttctac cactatcgtt atcagaaaca ttcgtatagc ctataggagc
    12901 cttgccaccc cattcaccat tctctctttt tgtatgtaaa gcacttctga ctttctcact
    12961 taatatatct acttgatttt gtgctactgc aatgccaata ttccatacga gtttatcact
    13021 tgcactagaa tgcttatgaa taattttatt ttcgttaata aaatgtattt caatgatctc
    13081 atcgttaata taattatcta acaaaggata ctctttaaaa cttctctgca atcggtctat
    13141 gtgtgtaacg actaaagcga tagttgaatg ttgtctgctg atataatcta gcattgctct
    13201 aaattctttt ctatctccac gagtagaaga ttctacaatc tgatatgttt tgataacatc
    13261 taaatttttt ctattacagt aatcggttaa ctgttcagtt tgtgtttgta tactgtagcc
    13321 atcttgctgt tccttacttg atacccttgc taaaattatt gctttcataa tactaattat
    13381 tgctcctttc ttacataaac tcaatcttat caatctcgcc catgagccgg ccggtctctg
    13441 tgagcgcgac gatgatcttc tggtagtgca tgatgtcttc aaatgagagc ctgcgtcctt
    13501 ttctgtcttt gagccatttc tgggcgggct ggtagccgcc gatgtaaaat tcccacgccg
    13561 tctgcgggac attatcaaaa tactgcgtct cgttgatcca gaccctgccg cccgcatatt
    13621 taaccctgcc gacctcgttg tccccgctct gcggatagtt ggttatacgc tgactgacca
    13681 cgggcgattc gagcaggtgg atttgcctta tctgcccgcc aagctcaacc agcttccaaa
    13741 aagtttctgc gtcttttgga taaggaaccc tcggaaaatc tatctttaaa aactccttaa
    13801 acttctccct gtaagaaggg gagtggagca cggcgtatat gtagtccaga atatctatgg
    13861 gcgcaaatgt attttcggtc tcctcttttt catttgtaaa tgtcatcccc agcccggcct
    13921 caatctcctt tactatatcc ggattcaaat tcggcgttct gccggtatta ccttccaatg
    13981 acccctgctc attgtctggg tagaggtaga gtgggaatac ataatttagt tcacctgtct
    14041 gcatagaaac attgcatctt tcagatattg tatttgaaag aaatatatgt tggaaatcaa
    14101 acgatgtttg tcttttacac aaaagtagag ctatattttt cttaaatagt aaatgtctca
    14161 tcacatcatg cctgggtctc cataatattc cttggctatt ttctgaatat agtgtatgtc
    14221 tgatatcgaa tggtctgtaa gatatcagtg ttttaacatg ttcactagca ctctctacat
    14281 cattttttac ttgtagtgtc ttgtgatgag ctaaattata ttttttggat atttcactta
    14341 catctaaatt caataagtct agctcgagtt gctttgtctc aatatcagtg aaatttataa
    14401 caaatttatc cttagctgtt tctatgcctg aactgtaaat cttcaacatt tcattaatag
    14461 gaaaatatga attatattca tcttcagaag caaaatcttt tggaacgaaa aaataataat
    14521 tatttttcgg atttaatctc ttccattcaa ttgattttat atcagattca aacaaatact
    14581 tatatttttc atcttcaatt ccatatacat cgcagtgata aatatctgta ggattatttc
    14641 tatcagtggt cttgataaaa atattaatac ttacaccgat tatgatatca aacacgttgt
    14701 cgtccttatc tccactaggt gttgtttcgt tttttctagt actaccatgc aagtctaaaa
    14761 tatatatttt attaaaacta tccattagag accttctcat aattctatga gtagtaccat
    14821 ctagaaaact attatttgat ataaatgcaa gtactccttc tccatttttt tctattatat
    14881 gttgggcata tcttataaac ttaatataat catcttcaat attatgtttt ctttcattca
    14941 accccttttt ataatcagta ataaggttct gaatccattc ccctttatta ctactactca
    15001 cactatacgg cggattgccg aggacgacca tcacgggggt gtcgcgtttt acctcgtttg
    15061 cctatttggc ctcgttggca agccatgttg aaaacaggct tccggtctcc tcgtgatact
    15121 cttcaagcgc attggtgagg taaaccttga gccgcttgtt agactggtgc ttgtagccgg
    15181 tctcttccaa aagcatgccg agcttgaggt gcgccatggc atagggagcc atgaggattt
    15241 caaagccgtg caggcgcggt atgaggtgct cgtccacgta attgttccag atgccctgct
    15301 gtccctcaaa gcatttgtgt atctgcctta tgacctcggc aaggaacgtg cccgtgccgc
    15361 aggcggggtc gagtatctgc accctgtgaa cctctttgtc tatgttcctg tagcccgagc
    15421 tgtaacgctg gtctgccacc tgcgtgtcct gctttagcgt gatcctggac gtgtcggcaa
    15481 gccctgccga aagcccgaac tcggtcttta aaatatcatc caccgcccgc actatgaagt
    15541 tcaccacagg ctccggcgtg taccacaccc cgcggctctt tctcagcttt gggtcatact
    15601 ccgagagaaa cgtctcgtag aaatgaataa tggggtcgtg ctgctgcgtg gccctgccaa
    15661 agttttgcag gagagccttt acgtctgtga gcctgaatat atcggcaagc gagttcacta
    15721 tccacttctg cctgtcatcc agatcatatc cggcgatgtc atgaaagagc tttcttaaga
    15781 aagggttagt cttcgggata agctctgccg cctcctgccg tgaaaaatcc ccgagcgtgg
    15841 gatcgtgaag acgcgcggca aacatgccgt aggcgatggt ctgggcatag atgtcggcaa
    15901 atgtcttcgg gtctatgtcg tgaatcaaca ccttcttaaa agcctcgagc tgctgtttga
    15961 tgtcactctc tgcgttttct tccaaatcct ttatcaatgc gttttcaata acatgcgcaa
    16021 acatccgcgc ctttgccgcc atgagctttg atagcctcga tgcggacttt atcgtctgcc
    16081 ccacgaaccg gcagaagtta gtgatgaggt ctgtgaagtg gtcgaaattt tcaggcatag
    16141 gaaggacgga gtttccttct ctctttccta tttccacggt ctctttaagc tcgccgtatt
    16201 cgtagaagcg gaattcgatg taatttgtga tgatgaggtt ttcgagcgcc gccctgtagc
    16261 ggtcgaactg ctctttgaat tccttattgt ctaggtcttt gtctatgtcc tttgcctcga
    16321 taaacccgat gtgtatgtcg ccctttgata tgagatagtc cggcgacccg cagtctgtga
    16381 ccttcgaggg ctcgtttgtg atgagatagc ccgccggcgc catcttcttt agcaggtcgt
    16441 gcaggtcggg cctgtagccg tgctcttttg tgtgtcctga ttgatgcctt ttattaagat
    16501 catttatata ttctgcgagc ataattaatt taaaacctta tgtttatttg attgaaatac
    16561 atcagacatt tccaatttgt taaatttatt taaatctgca tatacttttt taaacatgtc
    16621 tgtcttatta acagacttga aatcaatatt atcaagatca gattttaaat tgtaccacga
    16681 cctgtctgat tggttatcaa gcaaattcct cagacccttg gatcctattg attcaattat
    16741 atagatagcg ccaattaatt gaagtatttt tgaattttta tagtttgcag ctttatttat
    16801 ttgaataaag taatcctctg gctttatttg tttatccaca cctataaacc attcatcaaa
    16861 tatagcatca aaatggtaaa gtaagatttt cttagaaata gtactattaa ataaattgtt
    16921 ggatgtaatt cccgaattaa tacccgtctt tttcagtatg ttttctatag ttctcttatt
    16981 acccaatctt atctccattc taataacatc agggttatta gtttctttat atttcttgaa
    17041 taaatcaagc tgcatgtcat tatccctttc tattgctctt ttatctgaca ccttagcctt
    17101 ttcgaggtct ttaatcttat cataaaacac tatttgatat gagttagtat gataacgtaa
    17161 agcatgaccg ccatttctaa aatctgtagt gcttaaatct aatcgtttac tgatgttaac
    17221 tttatttaat tcagataata tcatggcact attcaggtgt ttatccaaga ttatattctt
    17281 tgagtaatga atcgaagata ctttggcatt tttcagtgca tcaatagtag taacaacacc
    17341 cataattaat aattgatctt ttagcttgta aatcagccta tcaaaatcgt tatcttccag
    17401 ttcatcgaag ttatttccaa ataatagttt aggagctgaa aactctattt tgagctgaac
    17461 aaaaaagcca ttaggtttat ttctcataaa aagtgtgagt ctgggtaaat atccatgcct
    17521 ttttttatca tctt
//
LOCUS       DBNGSV010000278         1204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_603957, whole genome shotgun sequence.
ACCESSION   DBNGSV010000278 DBNGSV010000000
VERSION     DBNGSV010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_603957"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="AC159U_10330"
     CDS             <1..783
                     /locus_tag="AC159U_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3337488.1"
                     /translation="MDENFIALNVHDDQENAVEVFKKYDRIALPVVDKLGILIGIVTV
                     DDVLDVAEEEATEDIQKIAGVAALEEPFPTISIFSMVRKRAIWLTILFVAQILTAVAM
                     GFFENEISKAVVLSLFIPLVISSGGNSGSQAATLVIRAMAVGEIALADWWLIMRREIL
                     SGLMLGGILGIIGFLQVAILTNIFHVISSYWVLVGVTVFFSLIGIVLWGTISGSMLPF
                     ILKKLGADPATSSAPLIATLCDVTGLVIYFSVAIMVLSGTLL"
     gene            871..>1204
                     /locus_tag="AC159U_10335"
     CDS             871..>1204
                     /locus_tag="AC159U_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3337489.1"
                     /translation="MKGIILAGGLGSRLYPITQVYSKQLALIYDKPLIYYPLSILMLG
                     GIKDVLIISNADTIPLYQKLFGDGSQLGMKFNYVVQPEPKGIAEAFILGEEFIGLDRV
                     CLILGDNIF"
BASE COUNT          339 a          185 c          254 g          426 t
ORIGIN      
        1 atggacgaga atttcattgc ccttaatgtg cacgatgatc aggaaaatgc agttgaagtt
       61 ttcaaaaaat atgaccgtat tgcgcttccg gttgtagata aattgggtat tcttattggt
      121 atagtaactg tagatgacgt acttgatgtg gctgaagaag aagcaactga agatattcaa
      181 aaaattgccg gtgtagcagc attagaagaa ccgtttccta ctatttccat tttctctatg
      241 gttagaaaga gagcaatttg gttaacaata ttatttgttg ctcaaatact tactgccgtt
      301 gcgatgggat tttttgaaaa tgaaataagt aaagcagttg tactttcttt atttattcct
      361 ttagttattt caagtggtgg aaactcaggt tctcaggcag ctactcttgt gatcagggcg
      421 atggctgtgg gtgagattgc tcttgcagac tggtggttaa taatgcgtcg tgagatcctc
      481 tcaggtttaa tgttgggcgg aattcttggt attataggat ttttgcaggt ggctatcctt
      541 acaaatatat tccatgttat ttcatcttac tgggttctgg tgggggttac agtgtttttt
      601 tcacttatcg gaattgtact ttggggaact atctcaggtt caatgctacc atttatctta
      661 aagaaacttg gggctgatcc tgcaacttcc tcagcaccct taatagctac actgtgcgac
      721 gttacgggtc ttgtaattta tttttcagta gctataatgg tattaagcgg tacactgcta
      781 taatcgcgat gttcaaatta ttaatctgga aaatctaatt gtttcttata tttgtttcta
      841 aataataatc agaaataaag ttaaaaaaat atgaagggaa taatacttgc tggtggactt
      901 ggttccaggt tgtatcctat tacgcaagtc tatagtaaac agcttgccct tatctatgac
      961 aaaccactaa tttattatcc gctttcaatt ttaatgttag ggggtataaa ggacgtacta
     1021 atcatttcaa atgcggatac cattccattg taccaaaaac tttttggtga tggttctcaa
     1081 ttgggaatga aatttaacta tgtggttcaa cctgaaccaa agggaattgc tgaagcattt
     1141 attcttggag aagaatttat tggtttggac agagtctgcc ttattctggg tgataatatt
     1201 tttt
//
LOCUS       DBNGSV010000279         6211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_257524, whole genome shotgun sequence.
ACCESSION   DBNGSV010000279 DBNGSV010000000
VERSION     DBNGSV010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_257524"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="AC159U_10340"
     CDS             <1..295
                     /locus_tag="AC159U_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR3337490.1"
                     /translation="INDLIKKGLIDENPIKDYVAAVSVGIINGEPMLDLCYEEDSNAD
                     VDMNIVMTGTGDLVEVQGTAEGDPFSVDKLNELVSLGGSGIKELLKIQKSCIK"
     gene            313..906
                     /locus_tag="AC159U_10345"
     CDS             313..906
                     /locus_tag="AC159U_10345"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016201764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HIR3337491.1"
                     /translation="MIEQLVIATTNKGKIKEFRSLLGKNVKNILSINDYDNIPKIEET
                     GSSFSENALIKARAVANITGKLTISDDSGLEVDALSGEPGIYSARYAGDGASDSDNIE
                     KLLEELFRVDDRAARFVCCIALVDDKGNEHTFTGTCEGEILTSARGDGGFGYDPVFYY
                     PDADKTFAELSLEEKNKYSHRAKAINKLNEYLSTLKK"
     gene            955..2556
                     /locus_tag="AC159U_10350"
     CDS             955..2556
                     /locus_tag="AC159U_10350"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3337492.1"
                     /translation="MKKTKFIFVTGGVMSSLGKGLAAASIGCLLECRGLKVTLQKLDP
                     YINVDPGTMNPFQHGEVYVTDDGAETDLDLGHYQRFTNCQLSQKNNFTTGRVYDSVIS
                     KERRGEYLGDTVQVIPHITDEIKRRVLDLAEDDLIVIVEIGGTVGDIESLPFLEAVRQ
                     LRTDLGKENTLFIHLTYVPFLSAAGELKTKPTQHSVKDLLQIGIQPDILLCRTDRFLS
                     QDLKRKIGLFCNVEEKAVITAKDVDVIYDVPMIFHSEGLDDIITESLNIWTRKPQLDK
                     WNILSNKVKNLKGEVTIGVVGKYVNHQDAYKSLHEALAHGGFANNSKVQIKYVDSEDI
                     ENEGIDGLLSGVNGILVPGGFGKRGFEGKIDAITYARTNNVPFFGICLGMQMALVEYS
                     RNVCAIAGAHTIEYQDDVTNPVVHLMENQKSVDNKGGTMRLGAYPCIVKDDTIAYRAY
                     KTNNISERHRHRYEVNNKYRKRLEKKGLIFSGVSPDENLVEMIEIKEHPWFLGCQFHP
                     EFKSTPLSPHPLFVDFIKSASQFNN"
     gene            2610..2768
                     /locus_tag="AC159U_10355"
     CDS             2610..2768
                     /locus_tag="AC159U_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337493.1"
                     /translation="MSLKLKVNEIVFFKKRNFISLACINELNKDQISVITEEGKQLNL
                     QVDQIVYC"
     gene            2886..4622
                     /locus_tag="AC159U_10360"
     CDS             2886..4622
                     /locus_tag="AC159U_10360"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HIR3337494.1"
                     /translation="MYDPQKSCGYNLQDIIELYTDKESSSLEKLQFVWALQRDEIYFK
                     KQDGLIYLNTPSESEEIIKRKKETLRKEQEKEQALDWIKGAHDGTAEVNKEDIEKYSD
                     YIELIKGYAVFIDKYERLKEAKALLNSVGIRDEIDALEFLINNGLWDKNEDPIFKRYG
                     IKQTYPQKVINQSEKLVNKEVNYDKLLDITKLELISIDDENTTDIDDALSVEIMGDKT
                     RVGVHISNVAAFIDEKDHLDNEALKRGETIYLAEGNISMFPSDLVTKLLTLSSKSTRK
                     ALSLFITFDPEFNISNYEFVQTKINVNKNISYNDANDNYLKTDQGSRLLAITNALRQK
                     RVKNGAIILQLPTMKVELDGDKVKSLTKYSMTTDSHNVIAEFMILLNNLAAKYFAENK
                     IPAVFRSQIESTSEEIKNLNRESEIFPVEVLRFLRPTKISTYAEPHKFLGIDAYVQIS
                     SPIRRYLDLVLQRQLVSCLNGTGPMYSNSALEKIYVRTEMAVKEKRTIEKSRERYWVL
                     KYLLDNNIKEVAGFISSVRDRRVNVYIPEYLIDVALTPSADNQLEVGSAAKINITKID
                     LFRRKISASFID"
     gene            complement(4692..>6211)
                     /locus_tag="AC159U_10365"
     CDS             complement(4692..>6211)
                     /locus_tag="AC159U_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3337495.1"
                     /translation="GIDNDGNGYIDDWRGWDFRNNDNNPADGHGHGTHTAGTTCAVGN
                     NDLGVTGVVWNCKIMALKFLSDGGSGSTSDAVEALQYATSFGVKVSNNSWGGSVFSTS
                     LSNAITASQSVGHIFVAAAGNNNRDTDSIPHYPSSYTHYNVISVASITDSGARSSFSN
                     WGHTSVDLGAPGSSIRSTLPGNTYSSGSGTSMATPHVTGVVALVYGLNPGASYTDIID
                     QIYRNVRPLASMNGITVTDGIADAHCAVIDCNGGGPTPTPPPPTPTPTPVPPTPTPTP
                     APDPTPTPTPAPPTPTPTPAPFTDYSVDTQQIIDGQIFSGSLADTFTDNDIDEVLREN
                     TDGGKPSNRISSLEKRFTINQNCDDNTLTINTFYDLDEADDVGTDDFIFEYDNGSGWT
                     EMFRIDTSDTSEQSYTYNNLPTPLTIRVIDSNGQTGNNGRDRVAIDYISLRCFNDGTP
                     PPPNPTPTPTTAPTPTPTPDPNQCIPTHSNEKGPRCSDGIDNDCDTFIDGDDPDC"
BASE COUNT         2112 a         1000 c         1333 g         1766 t
ORIGIN      
        1 gataaatgat ctgatcaaaa aggggcttat tgatgaaaac cctattaaag attatgtggc
       61 agcggtgagt gtagggataa taaacggtga gcctatgctt gatctctgtt acgaagaaga
      121 ctcaaacgcc gatgttgata tgaatatagt tatgacaggt actggggatc tggttgaagt
      181 gcagggtaca gcagaaggag atccatttag cgtagataag ttaaatgaac tagtaagtct
      241 tggtggcagt ggcattaaag agctattaaa aatccaaaaa tcttgtatta aataacttcc
      301 gaagtaaaga aaatgataga acaattagta atagcaacga ccaataaagg aaaaataaag
      361 gaattcaggt cacttcttgg aaaaaacgta aaaaatatat tatctataaa tgattatgat
      421 aatattccaa aaatagaaga aacaggtagc agtttctctg aaaatgcatt aataaaggca
      481 agagcagtag ccaatattac cggtaaactt accatttcag atgattcggg gcttgaggta
      541 gatgctttaa gcggagaacc aggtatttat tctgcaaggt atgcaggtga tggggctagt
      601 gacagtgaca atatagaaaa actattagag gagctcttta gggttgatga cagggctgcc
      661 aggtttgttt gctgcatagc acttgtggat gataaaggta atgaacatac atttactgga
      721 acatgtgagg gagaaatcct aacgtctgca agaggcgacg gcgggtttgg ttatgatcct
      781 gtattctatt atcctgatgc cgataaaaca tttgcagagc ttagcctgga agagaaaaat
      841 aagtatagcc accgtgctaa agcaattaat aaactaaacg aatacctatc aactttaaaa
      901 aaataataaa ctttatttga aacttatata gattcgaata tattaaaagc agggatgaaa
      961 aaaactaagt ttattttcgt tacaggtggt gttatgtcat cgcttggtaa aggccttgca
     1021 gcagcgtcga tcggatgttt gcttgaatgc agggggctta aagttaccct ccaaaaacta
     1081 gatccctaca taaatgtaga ccctggtaca atgaacccat ttcagcatgg agaggtttat
     1141 gtgacagatg atggggctga aactgatctt gatctcggcc actatcagag atttacaaac
     1201 tgccagttaa gccaaaaaaa taatttcact accggtaggg tatacgactc agtaatttca
     1261 aaggaacgaa gaggagaata ccttggagat actgtacagg taatacctca tataacagac
     1321 gaaataaaaa gaagggtatt agaccttgct gaagatgatc tgatagttat agtggaaata
     1381 ggtggtactg tgggagatat tgagagcctt ccgttccttg aagccgtaag gcagttaaga
     1441 accgaccttg ggaaagaaaa cacattgttc atccacctca cttacgttcc gttcctttcg
     1501 gcagcagggg agcttaaaac aaaaccaact caacacagcg taaaagacct tttgcaaatc
     1561 ggaatacagc cagacatttt actttgcaga accgaccggt tcttatcaca ggatctaaaa
     1621 agaaaaatcg gactattttg taatgtagaa gagaaggctg tgattacggc caaggatgtt
     1681 gatgtaatat acgatgtgcc gatgatattt cattctgaag gtcttgatga catcatcact
     1741 gaatctctta atatttggac aagaaagccg cagttggata aatggaacat actatcaaat
     1801 aaggtaaaaa atcttaaagg agaggtcact attggtgttg taggtaagta tgtaaatcac
     1861 caggacgcct ataagagtct gcatgaagca cttgctcatg gaggttttgc aaacaattca
     1921 aaggtacaga ttaaatatgt agattcagaa gatattgaaa acgaaggtat agatggtctt
     1981 cttagcgggg taaatggaat attggttccc ggagggtttg gaaagagggg gtttgagggc
     2041 aagatagatg caattacata tgcaaggacc aataatgtac ccttttttgg aatttgtctt
     2101 ggtatgcaga tggcgcttgt agaatattca agaaacgtat gtgcaatagc aggagctcat
     2161 actattgaat atcaagatga tgttacaaat cccgtagtac atttaatgga aaatcagaag
     2221 tctgtagata ataaaggcgg aactatgagg cttggtgcat acccatgtat agttaaagat
     2281 gatacaattg catatagagc ctataaaaca aataatattt cagaaagaca ccgtcacagg
     2341 tatgaagtta acaacaagta cagaaagagg cttgagaaaa aaggtcttat attcagcgga
     2401 gtctcacccg atgaaaacct tgtggagatg atagaaataa aagagcaccc atggttttta
     2461 ggatgtcagt ttcatcctga atttaaatcg actccgctca gtcctcatcc gctttttgtt
     2521 gattttatca aatcagcatc gcagtttaat aattaattat ctctatgatt taataaaatc
     2581 ccagagcagt tctttgtatt caatctacta tgtcattaaa acttaaagtc aatgaaattg
     2641 tattttttaa aaagagaaac ttcatatctc ttgcctgcat aaatgaattg aataaagatc
     2701 agatttcagt aattaccgaa gagggaaaac agcttaattt gcaagttgat cagattgttt
     2761 attgttaagg agtttgttca gataaaacag ctaacagctc agaaattaaa cttgaactga
     2821 gggccctcag aagacagctt gacgaaaaca aagaaaaagt cgatttaaaa accatatggg
     2881 actgcgtgta tgatcctcaa aaaagttgtg gttataacct gcaagatatt attgagctat
     2941 atacagacaa agagtcaagc agtttagaaa aactgcagtt tgtctgggcg ctgcaaagag
     3001 acgagatcta ttttaagaag caagacggtt taatctactt aaatacacct tcagagtctg
     3061 aagaaattat taaaagaaaa aaagaaaccc tgagaaagga acaggaaaaa gaacaagcct
     3121 tagactggat aaaaggcgca catgatggga cggctgaagt taataaagaa gatatagaaa
     3181 aatattcgga ttatatagaa cttataaaag gatatgcagt ttttatagac aaatatgaaa
     3241 gattaaaaga agcaaaggcg ctactgaatt ctgtaggtat aagagatgag atagatgcgc
     3301 tcgaattttt aattaataat ggactatggg ataaaaatga agacccgata tttaaaaggt
     3361 acggcattaa acaaacatat ccgcaaaaag taataaacca gagtgaaaaa ttagtaaata
     3421 aagaggttaa ttacgacaaa ttacttgata ttacaaaact cgaactcatt tcaatagatg
     3481 atgaaaatac cacagatatt gacgatgctt tatctgttga gatcatgggt gataaaacca
     3541 gggtaggggt tcatatatca aatgtggcag cttttataga cgaaaaagat cacctggata
     3601 atgaagcatt aaagagagga gaaactatat atttggctga gggcaatata agtatgtttc
     3661 cctcggattt ggtaacaaag cttctcactc taagctcaaa aagcacaaga aaagcactca
     3721 gtttgtttat tacctttgat cctgaattta acatatcaaa ctacgagttt gtgcagacaa
     3781 aaattaatgt aaataaaaat ataagctaca atgatgctaa tgataattat ctaaaaaccg
     3841 atcagggaag cagacttctt gctataacaa atgcgctcag gcaaaaaaga gttaaaaatg
     3901 gtgcaattat attacagctt ccaacaatga aggttgagct tgatggtgat aaggtgaaat
     3961 cactcacgaa atattcgatg acaaccgatt cccataatgt tatagcagaa tttatgatcc
     4021 tacttaataa ccttgcagca aagtattttg cagaaaacaa gataccggca gtattcaggt
     4081 ctcaaattga aagcacaagt gaagaaataa aaaatttgaa cagggagagt gagatattcc
     4141 ctgtggaggt gcttaggttt ttaagaccta caaagataag tacttatgct gagcctcata
     4201 agtttttagg cattgatgca tacgtgcaga tttcctcacc tatcagaaga tatcttgatc
     4261 tcgttcttca aagacagtta gtaagctgtc tcaatggtac gggacctatg tacagcaatt
     4321 ctgcacttga aaagatttat gtccgaacag aaatggctgt aaaagaaaaa agaactattg
     4381 aaaagtccag agaaaggtat tgggtactga aatatttatt ggataataat ataaaagagg
     4441 ttgctggatt tataagttca gtaagagaca gaagggtaaa tgtttatatt cctgaatatt
     4501 taattgacgt tgcactgact ccttcagcag ataatcagct tgaagtaggc agtgctgcaa
     4561 aaattaatat aactaaaata gatctattca gaagaaaaat atcagcttca tttatcgatt
     4621 aagacataaa aaaaggagag cctttttatg ggctctcctt ttttgtgatt aaactactct
     4681 aattttctat attaacaatc tggatcatca ccatctataa atgtatcaca gtcattatct
     4741 attccatctg agcagcgagg tcctttttca ttactgtgtg ttggtataca ctggttagga
     4801 tcaggtgtag gagtaggtgt aggtgcagtt gttggagttg gagttggatt tggtggtggt
     4861 ggtgtgccgt cgttaaagca tctgagtgat atatagtcta ttgctaccct gtctcttcca
     4921 ttatttccag tttgtccgtt tgaatctatt actctgattg taagcggcgt tggaagattg
     4981 ttataagtat atgactgttc agaagtgtca gatgtgtcta ttctaaacat ctctgtccag
     5041 cctgaaccat tatcatattc aaatataaaa tcatcagtac caacatcatc agcttcatct
     5101 aaatcataga atgtgttgat ggttaatgtg ttatcatcac agttctgatt aattgtgaat
     5161 cgtttttcaa gagagcttat tctgtttgaa ggtttaccgc cgtctgtatt ttctctgagt
     5221 acttcatcta tatcattatc tgtaaatgta tcagccagag atcctgaaaa aatctgacca
     5281 tctattatct gttgggtatc cacactatag tctgtaaatg gagcaggtgt cggagtcggt
     5341 gttggtggag caggtgtcgg tgttggtgta ggatcaggag cgggtgtagg agtaggggtt
     5401 gggggaaccg gagtaggagt aggtgtcgga ggtggaggtg ttggtgttgg accgccgcca
     5461 ttacaatcta ttacagcaca atgagcatcg gcaattccgt ctgttacagt aatgccgttc
     5521 attgatgcaa gtggtctaac gtttctgtaa atctgatcaa ttatgtctgt ataacttgcg
     5581 ccaggattga gcccataaac tagggcaaca acacctgtta catgtggggt agccattgaa
     5641 gttccgctcc ctgatgaata ggtgttgcca ggcagtgtac ttcttattga gctacctggt
     5701 gctcctaaat caactgaagt atgtccccag tttgaaaatg aagatcttgc gccagaatca
     5761 gttatagatg ctacagaaat cacgttgtaa tgtgtatagg atgaagggta atgtggaatg
     5821 ctgtcagtat cacggttgtt attgccggca gcagcaacaa atatatggcc gacactttgt
     5881 gaagctgtga tagcattaga tagagatgtg ctgaaaacgc taccacccca gctgttgtta
     5941 gaaaccttaa caccaaaaga tgttgcatac tgaagcgctt ctacagcatc agaagttgag
     6001 cctgaaccgc catcacttaa gaattttaaa gccatgatct tacagttcca tactacgccg
     6061 gttactccta agtcgttatt acctacagca catgttgttc ctgctgtatg tgtaccatgt
     6121 ccatgaccgt cagccggatt gttatcatta tttctaaaat cccagcctct ccagtcgtct
     6181 atgtatccat taccatcgtt atctataccg t
//
LOCUS       DBNGSV010000280        10827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_50916, whole genome shotgun sequence.
ACCESSION   DBNGSV010000280 DBNGSV010000000
VERSION     DBNGSV010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_50916"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="AC159U_10370"
     CDS             complement(<1..267)
                     /locus_tag="AC159U_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3337496.1"
                     /translation="MIGLIRKYTKWLHTQWPAGIVEKLPQVNEDYSTNIPGLYIVGDL
                     TGIPLLKFSSDSGAKAVDSIIADSDFQNKKGKDHEVLDLVIIGAG"
     gene            complement(350..811)
                     /locus_tag="AC159U_10375"
     CDS             complement(350..811)
                     /locus_tag="AC159U_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3337497.1"
                     /translation="MSKLGFCSFIILSLFLSTNIFAATDLKINPKLRYSSDTNDGPLI
                     TGKDLNSAKLSSDKPSYVIFYHRECYNSKRQAKRIVELYDKYKEKVNFVVIDLDQIRN
                     NDQVKLKRKYYKNYIPHVTMISKGGSVLYDRAGEISTQHLFDILDKEIDKK"
     gene            complement(922..1605)
                     /locus_tag="AC159U_10380"
     CDS             complement(922..1605)
                     /locus_tag="AC159U_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3337498.1"
                     /translation="MGRIENYVNHATSNNAAAVINNNIGIRNKDNEIKLTDEQLIKDY
                     VENNNEQAFNLIIKRYENIVFGLAYRIIGNHSLCEEVMQEVFLILTKKLSTFREESKF
                     STWLYRVVVNVCYLYKKTEYKHTSNLRLDKEMNDDGDTKFIDNLEDKRACSPYEESKS
                     SEMIEMVERELKNLPEKYRVVFVLRDVDGLTNPEVAKILGITLAAVKSRILRARKFLK
                     SRLQNKLKI"
     gene            complement(1617..1886)
                     /locus_tag="AC159U_10385"
     CDS             complement(1617..1886)
                     /locus_tag="AC159U_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337499.1"
                     /translation="MLKITEINSSESKVELKLEGKIVGTCIDYLSEVCADFDKRQVEE
                     VLLDFSGVRYVESGGVENLQKLMKDNIKIKNCPIFIEELLKKSNL"
     gene            complement(2112..3653)
                     /locus_tag="AC159U_10390"
     CDS             complement(2112..3653)
                     /locus_tag="AC159U_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HIR3337500.1"
                     /translation="MTASTTRNNSCKNIEDRYRVLLDINNAIISYTDLDELLAKVAAK
                     LDEIFFYDVSTITLYDPEKNKLIMTAFGKLHGADSIAPGTELTMEGSHAGWVYKNNKP
                     LIVNDLSKRKRFEFDKTLYKEGIRSYVVVPLIAMDTTIGTLHLGSLSPERFKDVDREF
                     LLLIAKQVALAINNANCIKEIEELKEKVEGENIYLQEEIKLEHNFEEIVGQSKKLKEV
                     LKQIELVAETDSTVLIRGETGTGKELIARAIHNLSKRKDRPLIKVNCPAIPAGLIESE
                     LFGHEKGAFTTAIAQQIGKFELADCGTIFLDELGDLPLDAQAKLLRVIQEREFERVGG
                     SKHINVDVRIIAATNRSLEDAVSAGNFRSDLFFRLNVFPINLPPLRERKDDILLISKY
                     FIQKYMNKMGKDLKSVSDSTLNDLKNYHWPGNIRELENIIERGVILSTNDKLSIPKDM
                     FGSPISSEHSYQTLDDLERNHIIQTLNDTGWQISGEKGAAKILGINPNTLRSKMQKLG
                     IEKPN"
     gene            complement(3745..4236)
                     /locus_tag="AC159U_10395"
     CDS             complement(3745..4236)
                     /locus_tag="AC159U_10395"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR3337501.1"
                     /translation="MLSKKIENALNEQIKYEYFSSYLYLAMSAYCDSQNFQGFANWMR
                     TQSTEEMTHALKLYDFVLNRDGNIKLKSIPEPPQKYTSIEKIFKQVYEHEIHVTGLIN
                     KLYELAIKENDHATAVELQWFIQEQVEEERSSKDIWEKLKLAGSNSSALLILDRELSE
                     RQV"
     gene            complement(4440..6608)
                     /locus_tag="AC159U_10400"
     CDS             complement(4440..6608)
                     /locus_tag="AC159U_10400"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR3337502.1"
                     /translation="MNTRYNIFPIICLLFFACSDVNENVPSEKIINEPGLYPISINHK
                     YGYIDKTGKIVIEPQFDFALSYFEDLAVVLSGDKFGFIDDKGQFEIEPQYDMAVPFQE
                     GVAEVLSGKQSKFINKNGAEIFKGEYEDVNYINEGLASFKKDDKYGIINKAGKVIIEP
                     KFDWISDFKEGLARVTIEKKKGYIDKRGKLVISAKYDYAYIFEEGLTAVKVKDKWGYI
                     DKSGGYVIKPRFEGASFWGFEDGQTSVMENGKWGLIDKKGNYIINPSSDSILKFSENL
                     APYVINNKKGYIDINGNIVIEPKYDDATMFKSGLAAVRVSGLYGYINTNGKFILKPQF
                     ENPNFFDENGITLAEYGGEKGFIDKNENWKIKLKPNLYCTLASEFHEEMSLIGVNGVF
                     EFGFINSKGELVYGQEGGDKKIPRHFSEGLAQIFKNGKWGYIDKTGHIVIEPKFDFAG
                     PFSEGLARIRTKEGSGYIDKSGSVVIEPKFNSVSEFSEGLAWAGGGYINKSGEFVIGP
                     HIDDNRITTYGFEIQEEQKKCNKNKEKKICKRVDFYDRGTNFSEGLASVCIRGRCGYI
                     DKTANKVIDFKFSRALKFSEGLARVTTDEKWGYIDKTGEIVIKEKYDFVRDFNSGAAM
                     VDTGDEGKKYGFINQKGEWIIEPSEHASLNYSEGLVFHSIGNPYLNPRYGYMDQNGEY
                     VIKPKYKDVSNFSEGLACVTTWGKSGYINTKGQWIWKPTR"
     gene            complement(6669..9839)
                     /locus_tag="AC159U_10405"
     CDS             complement(6669..9839)
                     /locus_tag="AC159U_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3337503.1"
                     /translation="MKLVESSILKPVSVVVGVIFVVLFGLISLFKIPVQLTPDVEKPR
                     ITVETRWIGGSPTEVETEIVREQEDELKTVQGLTEMTSESSDGSGKVILEFAVGTDID
                     ASLVDVSNKLEQVREYPSDADRPVISNVDVRSSAIAWFILKPLPGNETDINTYHDFTE
                     DFIKPRFERVPGVGSSNVFGGREREMQIIFNPNSLAARGITLLDMARAIDAENDNYSG
                     GAFDEGKRRYIVRTIGQYKSVKDIENVVITRKGGSPVYVKDVAKVKLGYKDPDFAVRQ
                     NGAAAIAVNAVKESGANTIDVMDGLLKAVDELNVGLLKDKGLYITNVYRETSYIVGAI
                     DLVKQNLIIGGMLAIAVLLLFLRSSSSTIVVATAIPISIIGTFIVMALLGRTINVISL
                     AGMSFAVGMVIDNSIVVLENIYRHMQMGKTRVQAAFDGATEVWGAVLASTLTTAAVFL
                     PVIFVQEQAGQLFKDIAIAISVSVVLSLIIAITVIPTFSSKILKIAKQQDGNNFFNLW
                     GHDAFAKNIAGYISSTVDKILSDNKLRLTVVGGFTFGAIFLSFLMFPKTEYLPTGNRN
                     LVMGILIPPPGYNIGEFTAIGKKIEKDLKPYWEAKPGSKEAKKLKGPTIKNFFYVARG
                     RSVFMGAVANDPGRVNELKPIMQSALRKIPGMIAIVLQRSLFQRGLGEGRNLNIQITG
                     PDIEKLVGLGQKIFFSVMKKFPDGQWRPIPSLDLGNPEVQVITNRERAADVGITNEEL
                     GFSVNALVDGVQVSDYLIGGDEIDLVIRGEDKYSSRTHEIENLYINSSTGELFTIGSV
                     AKVQVTNGPEQINHYERQRTIAIQVTPSEQMPLETAIDIVNKDILAPLKASGELGGEY
                     NVQLTGTADDLTVTRRALQWNFVLAAVIAFLLMASLFESFVYPFVIMFSVPLAAFGGF
                     LGLRILNIFTVQALDVLTMLGFVILIGIVVNNAILIVHQALNNMKEQGMANRDAISES
                     VKTRIRPIFMSTTTSVFGMLPLVLFPGSGSELYRGLGSVVVGGLVVSTIFTLFLVPSV
                     FSMVLDFRERLSIPSLNKIESKVVSE"
     gene            complement(9836..>10827)
                     /locus_tag="AC159U_10410"
     CDS             complement(9836..>10827)
                     /locus_tag="AC159U_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3337504.1"
                     /translation="VALVEEKVLNRPVSFVGNVVPFKRSVIASEVEGLVSNFPAYEGL
                     FVKQGEVLVEFKTSTLELNLKEAKASKQEAASRYTLARNNLKRIQNLHDKGVASIQEL
                     QDAQSERNAWFARLTQLKSQIEQDEYDLGVSRIKAPFTGFVVAKHTEVGQWVEEGGQV
                     LELIEIDKLKIVINIPENFVGKLKHEDRVIVNFDALPGNDFDAKVDAIVPQADRESRT
                     FPIIIVLQNKDYLIKSGMVARASFLIGDEQPVKLVPKDAIIEFNKSKMVYVVNEGAAQ
                     PVPVSPGFAFEDMIQVSGELLKGQQVVIRGNERLRPNQPVKIINGSDSPQEAS"
BASE COUNT         3068 a         2423 c         1652 g         3684 t
ORIGIN      
        1 tccggcacca ataatcacaa gatccagaac ttcatgatcc ttaccctttt tattctggaa
       61 atcagagtca gcaattatac tatcaacagc tttagcacca ctatcagatg aaaatttgag
      121 aagaggaata ccagttaggt caccaacgat atatagcccg ggtatatttg tggaataatc
      181 ttcatttacc tgaggcagtt tttctacaat tccagccggc cactgggtat gaagccattt
      241 agtgtacttt cgtatcagac ctatcaatac agcctccgtt tcctctttat cgtaatacag
      301 ttacagcaat cattttactt aactattgac catgtgttaa caaatctttc tattttttat
      361 caatctcttt atcgagtata tcgaagagat gttgagtact tatttctccg gctctatcgt
      421 acagaacact tccaccttta ctgatcatgg ttacatgcgg tatatagttt ttatagtact
      481 ttcttttgag cttaacctga tcgttgtttc ttatctgatc caaatcgatc acaacaaaat
      541 tcactttttc tttatactta tcatacagct ccacaattct tttagcctga cgtttggaat
      601 tatagcactc tctatgataa aaaatcacat aagagggctt gtccgaactg agttttgcgc
      661 tgtttaaatc cttaccggtt ataagcggcc cgtcgtttgt gtcgctgcta tatcttagtt
      721 taggattaat ttttaggtct gttgctgcaa aaatgtttgt ggaaagaaaa agtgacaaaa
      781 taataaaact gcaaaagcct aatttactca tattttctcc gtacatgatt agtatattag
      841 caacgagtta cataacccag cgtacaataa cccacattca ttatattatt ataatatgag
      901 ctaaatttca tcaaacagga ttcaaatctt gagtttattt tgcagtctgg acttaagaaa
      961 ttttcttgcc cttaaaattc gtgacttaac ggctgcaagt gtgataccaa gtatttttgc
     1021 aacttctggg tttgtaagcc catcaacgtc tcttagtaca aaaaccactc tgtatttttc
     1081 gggcagattt ttcaactccc tttcaaccat ctctatcatc tcagagctct tactttcttc
     1141 ataagggcta caggctcttt tgtcctcaag attatctata aacttagtat ctccatcatc
     1201 attcatttcc ttatccagcc ttaagttgct tgtgtgtttg tactcggttt ttttgtacag
     1261 ataacataca ttaactacta ccctgtatag ccatgttgaa aatttcgact cttctctaaa
     1321 tgtggagagt ttctttgtaa gaattaaaaa gacctcctgc attacctctt cacaaaggga
     1381 gtgatttcct attattctat acgccagtcc aaaaacaata ttttcgtacc tcttaataat
     1441 aaggttgaat gcctgttcat tattattttc tacataatct tttataagct gctcatcggt
     1501 aagcttgatt tcgttatcct tatttctgat accgatatta ttgttaataa ccgccgctgc
     1561 attgttgctt gtagcatgat ttacataatt ctcaattctt cccatttgat tactccttat
     1621 aaattcgatt tttttaacaa ctcttcaata aagatagggc aattttttat cttgatattg
     1681 tctttcatta atttttgcaa attttctacc ccgccagact ccacatatct gacaccggaa
     1741 aaatcaagca atacctcttc aacttgcctc ttatcaaaat ccgcacaaac ttctgacaga
     1801 tagtctatgc atgtgccgac tatcttgccc tcaagcttaa gttcaacctt actttctgaa
     1861 ctgtttattt ctgtaatttt aagcatctct tacctttaat ttactcttca gtattaattt
     1921 acgtttactc atataagtac aataagcgtg ccagggctgg aaaaaaagaa attactgata
     1981 taactatctg taataattaa atattctgca acaagaaatg atatttcgat atagaatgaa
     2041 aatacgatat gccgtaacat ttacgatata tcatgaattt ggtttgtttg gcttgttaag
     2101 tgaaggtttc gttaattagg tttttcaata ccgagtttct gcattttact tctaagggtg
     2161 ttaggattta taccaagtat tttggcagct cctttttcac cgcttatttg ccagccggta
     2221 tcatttaggg tctgaatgat gtggtttctt tcaagatcat ccagtgtttg gtaactatgc
     2281 tcggaagata ttggggatcc aaacatgtct tttggaatac ttagcttatc gtttgtagaa
     2341 agtatgacac ctctttctat gatgttctca agctctctga tatttccagg ccagtgatag
     2401 ttctttagat catttaaagt ggaatcgctt acagatttaa gatcttttcc catcttgttc
     2461 atgtactttt gaataaagta tttagaaata agaagtatat catcttttct ctctctaaga
     2521 gggggaagat taatgggaaa cacatttaac ctaaagaaca aatcacttct aaaatttcct
     2581 gccgaaactg cgtcctcaag gctcctgttt gttgcagcaa ttattcgtac gtctacattg
     2641 atatgtttac ttccccctac cctctcaaac tccctttcct gaatcactct gagaagcttt
     2701 gcctgagcat cgagcggcag gtcaccaagc tcatccaaaa aaattgtgcc gcagtcggca
     2761 agctcaaatt tacctatttg ctgagctatt gcagttgtaa atgcaccttt ttcatgaccg
     2821 aatagttcac tctctatcaa ccccgccgga attgccgggc agttcacttt aattagcggt
     2881 ctgtctttcc ttttgctcag attatgaata gctcttgcta tcagctcttt gccggttcct
     2941 gtttctcccc ttataagcac cgtgctatca gtctctgcca caagctctat ttgttttagt
     3001 acttctttta gtttcttgct ctgtcccaca atctcttcaa aattatgctc aagtttaatc
     3061 tcctcctgaa ggtatatatt ttccccttcg accttttcct taagctcctc aatttctttt
     3121 atgcagttag cattattaat tgcaagcgca acctgttttg ctattagcaa aaggaactct
     3181 ctgtcgacat ctttaaatct ctcagggctc aaactgccca gatgaagcgt ccctatagtt
     3241 gtatccatgg ctataagtgg tacgactaca tatgatctaa taccttcttt atatagagtt
     3301 ttatcaaact caaatcgttt tctcttggat aagtcattta ctataagagg tttattattc
     3361 ttataaaccc atcctgcatg gcttccttcc atagtaagct cggtaccagg cgcgatgctg
     3421 tccgctccgt gaagtttacc aaacgcagtc atgataagct tatttttttc aggatcgtat
     3481 agagtaatgg tagaaacatc gtagaagaaa atttcatcca gctttgctgc gaccttggca
     3541 agaagctcat ctagatcagt ataggaaata attgcgttat ttatatctag taaaacccta
     3601 tacctgtctt ctatattctt acagctgtta ttacgggttg tacttgcggt catataagtt
     3661 tattagatag ataaattaaa aaaaaagagg cttaaaaaag cctctttttt atgaaattta
     3721 ttaaaggagg taaatacagt tgtgttagac ttgtctttcc gataattccc ggtcgagtat
     3781 gagtaatgcc gagctattag agccggcaag ttttaatttt tcccaaatat ctttagaact
     3841 tctttcctct tctacctgct cctgaataaa ccactgcagc tcaactgctg tcgcgtggtc
     3901 attctcttta atcgcaagct catagagttt atttattaat cctgttacat gaatttcatg
     3961 ttcataaacc tgtttaaaaa tcttttcaat agatgtatat ttttggggag gttccggaat
     4021 tgatttgagc ttaatgtttc cgtctctgtt taagacaaaa tcatagagct ttagagcgtg
     4081 agtcatctct tcagtgctct gagttctcat ccagtttgca aatccctgaa aattttgaga
     4141 atcgcagtaa gcagacatgg caagatatag gtaagatgaa aaatactcat attttatctg
     4201 ttcattcagt gcattctcaa tttttttact taacatatta cttttttccg cctccgggtt
     4261 aaaaatagtt tgctttttta ctgtacacta tagcttggta ataactttac catataaaat
     4321 attgttaata aaggatttta tgaaaatttt tttaacaatt gatcaaaaat cagaaagaaa
     4381 tacgaaaatc cagtcctgta ttgcgattta tatgtctcta atagcagaat taagagattc
     4441 taccgagtag gcttccaaat ccactgccct ttggtgttta tgtagccgct tttgccccag
     4501 gttgtgacac atgccagacc ttctgaaaaa ttagatacat ctttatattt tggctttatt
     4561 acatactctc cattttgatc catataacca tatctggggt tcaaataagg atttcctata
     4621 gaatgaaaaa ccaagccctc actataattc aggcttgcgt gctcgcttgg ttctataatc
     4681 cactcacctt tttgatttat aaatccatat tttttaccct catcaccagt atctaccata
     4741 gcagcacccg aattaaaatc acgaacaaaa tcatattttt ctttaattac tatttcgcca
     4801 gttttatcta tgtatcccca tttttcatca gtggtaaccc ttgctaaccc ctcagaaaat
     4861 ttcaaagctc tgctgaattt gaaatctatg accttatttg ctgttttatc tatatatccg
     4921 catctccccc tgatacaaac tgaggctaat ccctctgaaa aatttgtacc ccgatcataa
     4981 aaatctactc tcttacaaat ttttttctct ttattcttat tacatttttt ttgttcttct
     5041 tgtatttcaa atccgtaggt tgtaattcta ttatcatcaa tatgaggtcc tatgacaaat
     5101 tcgccagatt tatttatata tccaccccca gcccaggcaa gaccttctga aaattcactg
     5161 actgaattaa attttggctc tataactact gatccggact tatcaatgta gcccgatcct
     5221 tccttagtcc ttattcttgc taacccttct gagaatggac cggcaaaatc aaatttaggt
     5281 tctattacaa tatgtccggt cttgtctata tagccccatt ttccattttt aaatatttga
     5341 gcaagtcctt cagaaaaatg tcttggtatt tttttatctc ctccttcctg cccatatact
     5401 aattctcctt tactgtttat aaatccaaac tcaaatacac catttacacc tattagagac
     5461 atctcttcat gaaattctga agcaagcgtg caatatagat taggttttaa tttaattttc
     5521 cagttttcat ttttatcaat aaatcccttt tcaccaccat actcagccaa agttattcca
     5581 ttctcatcaa aaaaattagg gttttcaaac tgaggtttta atataaattt tccattagta
     5641 tttatatatc catacaaacc actaaccctt actgcagcaa gacctgactt aaacattgtt
     5701 gcatcatcgt actttggctc tataacaata tttccattta tatctatgta tccttttttg
     5761 ttatttatta catatggagc gaggttttca gaaaatttta aaatactatc tgaactagga
     5821 tttattatgt aattaccctt tttatcaatt aatccccatt taccgttttc catcacagaa
     5881 gtttgtccat cttcaaagcc ccaaaaactt gcaccttcaa acctgggttt aataacataa
     5941 ccccctgatt tatcgatata tccccattta tctttaactt ttacagctgt aagtccttct
     6001 tcaaaaatat atgcataatc atacttggca ctaataacaa gtttgcctct tttatcaata
     6061 tatccctttt tcttttcaat agttactctg gccagtcctt ctttgaaatc cgaaatccag
     6121 tcgaacttcg gttctataat tacttttcct gctttgttga taatcccata tttgtcatct
     6181 ttcttaaatg atgccaagcc ttcatttata tagttaacat cttcatattc acctttgaaa
     6241 atttctgcac cattcttgtt tatgaatttg gactgttttc ctgaaagaac ttctgcaaca
     6301 ccctcttgaa atggaacagc catatcatat tgtggttcta tttcaaattg tcccttatca
     6361 tctataaatc caaatttatc accacttaac acaactgcca ggtcttcaaa ataacttagt
     6421 gcaaaatcaa actgcggctc aatcacaatc tttccggttt tatctatata accgtatttg
     6481 tgatttatac ttattgggta taagcctggt tcgttgataa tcttttcaga aggaacattc
     6541 tcattaacat cagagcaagc aaaaaacaac aagcaaataa ttggaaagat attatatcta
     6601 gtattcatat actatattat ataacaatta atagataaac ttccagaggg tcaaacacaa
     6661 atatctaatt actcactaac cactttcgat tcaattttat ttagggatgg tatggacaac
     6721 ctttccctaa aatccaagac catgctaaaa acagaaggga ctagaaaaag ggtaaagata
     6781 gttgagacaa caagccctcc aacgaccaca cttccaagcc ctctgtaaag ctcagaacct
     6841 gaacccggga aaagtacaag cggaagcatg ccgaatacgc ttgtcgtggt gctcatgaag
     6901 attgggcgga ttctggtttt gaccgattct gatattgcat ccctgtttgc catgccctgc
     6961 tctttcatgt tatttagggc ttgatggaca ataaggattg cgttatttac aacaattcct
     7021 attaagatca caaacccaag catcgtgagt acatcaagtg cttgaactgt aaatatattt
     7081 agaatccgga gtcctaaaaa tccgccaaat gcagcaagcg gcacgctgaa cattatcaca
     7141 aacggatata cgaagctttc aaaaagtgaa gccataagaa gaaatgctat tacagctgca
     7201 agaacaaaat tccactgaag tgccctcctt gttacagtaa ggtcatcggc agttcccgta
     7261 agctgtacat tatactcccc gccaagctct cccgatgctt ttaacggagc cagaatatcc
     7321 ttgttaacta tgtctattgc agtctcaagc ggcatctgct ctgatggagt cacctgaatt
     7381 gcaatagtcc tctgcctctc ataatggttt atctgctccg ggccgtttgt aacctgcact
     7441 tttgctacag aaccaattgt aaagagctct cccgtgctgg aattaatata caagttctct
     7501 atctcatgag tcctgctcga atatttatcc tcacctctaa taacaagatc aatctcatcc
     7561 ccaccaatca ggtaatcact cacctgcacc ccgtccacaa gcgcatttac gctgaaacca
     7621 agctcttcat tcgtaatacc cacatcagca gcacgttccc tgtttgtaat tacctgtacc
     7681 tcggggtttc caagatcgag gctgggaatc ggacgccact gtccgtcagg aaattttttc
     7741 attactgaga aaaatatttt ttgaccaagc cctacaagct tttcgatatc aggccccgta
     7801 atctgaatat ttaaatttct tccctcacct agccctcgct ggaaaagact acgctgtaaa
     7861 actattgcaa tcattcccgg aatcttcctt aatgcagact gcattatagg ttttagttca
     7921 ttaaccctgc caggatcatt tgcaactgct cccataaata cacttcttcc ccttgctaca
     7981 tagaaaaaat ttttaattgt tggccctttt aatttttttg cttcttttga tcctggttta
     8041 gcttcccaat atggtttcag atccttttca atttttttgc ctatcgctgt aaattcacca
     8101 atgttatatc ccggtggtgg aattaaaatt cccataacca aattcctatt tcccgtaggt
     8161 agatattcag ttttaggaaa cataagaaaa ctaagaaata ttgctccaaa agtaaatcct
     8221 cctacaacag tcagcctcaa tttattatca gacaaaatct tatcaacagt actggaaatg
     8281 tatccggcta tatttttagc aaatgcgtca tggccccata gattaaaaaa attgtttcca
     8341 tcttgttgct tagcaatctt caatatttta gatgagaacg ttggtataac agtgatcgcg
     8401 attataaggc ttagaacaac ggaaacactt atagcaattg caatgtcctt aaaaagctgc
     8461 cccgcctgct cctgcacgaa tatcacggga agaaaaacag ctgctgtagt aagagtactt
     8521 gcaagcacag ctccccaaac ctcagttgcc ccatcaaagg ctgcctgaac tctggtctta
     8581 cccatctgca tatgacggta gatattttct aatacaacaa tggagttgtc tatcaccatg
     8641 ccgacagcaa agctcattcc ggcaaggcta atcacattta ttgtcctgcc taataaagcc
     8701 ataacaataa acgttccgat gatactaatt ggaatagcag ttgctacaac tatcgtactg
     8761 ctggagcttc ttaggaacag taaaagtacg gcaatggcaa gcataccgcc gataatgagg
     8821 ttttgtttaa caagatctat agctcctact atataactgg tctctctgta aacattagtt
     8881 atataaagcc ctttatcctt tagcagtccc acattaagct catccacagc tttcagtaga
     8941 ccgtccataa catctattgt gttagcaccg gattccttta ccgcatttac ggctatagca
     9001 gctgctccat tttgccttac tgcaaaatcc ggatctttgt acccaagttt gacctttgcc
     9061 acgtctttta catatacagg tgagccgcct tttctcgtta ttactacatt ttcaatatcc
     9121 tttaccgatt tgtactgccc gattgttcta actatgtatc ttctcttccc ctcatcaaac
     9181 gccccgccgc tgtagttatc attctcagca tctatagccc ttgccatgtc taacagagtt
     9241 attccccttg cggcaaggga attgggatta aagataatct gcatctccct ttcccttccg
     9301 ccaaagacat tggacgaacc aactcccggc accctttcaa atctgggttt gataaaatcc
     9361 tcggtaaaat catgatatgt gtttatgtcc gtttcatttc cgggcagagg ttttaaaata
     9421 aaccaggcaa tggcgcttga tcttacatcc acattgctta tcacaggcct gtctgcatca
     9481 gaagggtatt ctcttacctg ttccaactta ttggatacat ccacaagtga agcatcaata
     9541 tcagtaccaa ctgcaaactc caggatcact tttccactgc cgtcagagct ttcacttgtc
     9601 atctcggtaa ggccctgaac agtcttaagc tcatcttcct gttcccttac aatttcagtc
     9661 tctacctcag taggactacc ccctatccac cttgtttcca ctgtaatcct tggtttttct
     9721 acatcggggg taagctgaac aggaatcttg aatagagaga ttaatccaaa aagaacaaca
     9781 aagattacac ccacaacaac acttaccggc tttagtatag aagattcaac tagtttcatg
     9841 aggcttcttg tggactgtcg cttccgttaa ttatctttac aggctggttt ggtctaaggc
     9901 gttcatttcc tcttattact acctgctgtc cttttaaaag ctcgccgctt acctgaatca
     9961 tatcctcaaa tgcaaacccg ggacttacag gaacaggttg tgccgcacct tcatttacaa
    10021 catagaccat cttgctttta ttaaattcta ttatcgcatc ttttgggaca agttttactg
    10081 gctgctcgtc gccaattaga aatgaagccc ttgcaaccat gccgcttttt attaaataat
    10141 ccttattttg cagaactatt attataggga aagtgcgtga ctccctgtca gcctgtggga
    10201 caattgcgtc tactttagca tcaaaatcat ttccgggaag cgcatcaaaa ttcacaatca
    10261 ctctgtcttc gtgttttaac ttacccacaa agttttccgg tatgtttatt acaattttga
    10321 gtttgtctat ttctataagc tcaagcacct gtccaccttc ttctacccac tgaccaacct
    10381 cagtgtgttt agccactaca aaccccgtaa aaggcgcttt tattctggaa accccaaggt
    10441 catattcatc ctgctcgatc tgtgatttga gctgtgtaag ccttgcaaac catgcatttc
    10501 tttctgactg cgcatcctgt agttcctgga tggatgctac ccccttatca tgcaggtttt
    10561 gtatacgctt tagattgttt cttgcaagtg tataccttga ggctgcttcc tgttttgagg
    10621 ccttggcttc ttttaaattt aactcaagcg tacttgtctt aaactccacc aatacctcgc
    10681 cctgctttac aaaaagccct tcataagctg gaaaattact cactagtccc tcaacctcac
    10741 ttgcgattac acttctttta aatgggacaa catttcccac aaaactcacg ggtctgttga
    10801 gtactttttc ttctacaagt gcaacct
//
LOCUS       DBNGSV010000281         3881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1850652, whole genome shotgun sequence.
ACCESSION   DBNGSV010000281 DBNGSV010000000
VERSION     DBNGSV010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1850652"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1122
                     /locus_tag="AC159U_10415"
     CDS             331..1122
                     /locus_tag="AC159U_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337505.1"
                     /translation="MNNWESLKEKVDNIVIDLEVSHDNIESYPQIERLVYDKLTELNI
                     DRKDSSLSNYVKSTKKIIYYLDKYSSYIISFKHNDNKQDYQLYSKNINQLFAVVANEY
                     NDINKKFSNSSGIEKLGLGDCLVDASLLYLKLSQELLQLPSPEYRAKYREIIKTLKNI
                     KQLDIDLLKNIYDLSKGEQTLINKNSLKDNGLKDIILDYLKQKRYIDLTSEETGITEK
                     GQKIMKRQSESSGNITINNIYQVFGDIESAQLQINSTDSKQEIKK"
     gene            1145..1777
                     /locus_tag="AC159U_10420"
     CDS             1145..1777
                     /locus_tag="AC159U_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337506.1"
                     /translation="MQKKVQRLKFLEKLYKLSNGKSNVYFNTYYTKIAEPLGFDIETA
                     TEIFAYLKHEGLIAVTGGTKIALTHKGLLEYESAMTNPEKDTEHFPAGIINNYLNVGS
                     NINNMQIQIGTKDSIQTQSITIENSNELIELLEEILNKKDDYKLDDVDEEELIADITT
                     AKTQIESSRPKIEIIKTSLDSVKRILESAVGSALANQTVQKLIPFIESLG"
     gene            complement(2237..3265)
                     /gene="tsaD"
                     /locus_tag="AC159U_10425"
     CDS             complement(2237..3265)
                     /gene="tsaD"
                     /locus_tag="AC159U_10425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3337507.1"
                     /translation="MSSLILAIESSCDETSASVIKNGSEILSNVVSSQVDIHKKYGGV
                     VPEIASRKHVEAIIPIINESVEEANVKLNELNAIAVTNGPGLIGSLMVGLSTAKSVAF
                     SLDVPLIAVDHIEAHAYSVNIEHELTFPYIALVVSGGHTSLYKVNSYKSLELIGNTRD
                     DAAGEAFDKGAKLLGLDYPGGIQIDKLSKNGNKKKYDFPRPFLKTDSFDFSFSGLKTA
                     LVHFVKKNEITSDEMLCDVCASYQEAIAETLIVKTLRAAEEFNISTVAFAGGVAANSR
                     IRELGKEKLSENNIDLFIPHIKYCTDNAAMIARLAAEKFANNEFSDLEVDVYSTKRSS
                     YTRGKGFK"
     gene            3380..>3881
                     /locus_tag="AC159U_10430"
     CDS             3380..>3881
                     /locus_tag="AC159U_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811927.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3337508.1"
                     /translation="MKQKNSKDKIPQAEVISREPFPNSNKTYVEGQNHDIRVGMRDID
                     VTGTLGNGSDSAYITVYDTSGPYTDPEVDINVLKGLDPIRAGWIKDRGDVEQLEDFSS
                     EFSRNSAKNGNGITFKNLRKPLKAKKGSNVTQMHYARKGTVTPEMEYIAIRENQQIDR
                     LGEKYKE"
BASE COUNT         1407 a          651 c          679 g         1144 t
ORIGIN      
        1 gaaaccatag aggactggct aaacacaaga gaattaatta cttcaaatgg tacacctttt
       61 taaagcaaat ctgactcaaa ttagcacaca ttttttgcaa ttatgcccga aatgacatag
      121 taataaaatc ccagttcacc caaaaggtca aaagttcaaa agacaagggt atagcccctg
      181 aacttttgac cttttgaccg tattgtaggg ctttggtatt agagggggca gtcggggaac
      241 tgcacgtatt cactattagg gcaagagggg gttgaaatta taaatccgat gtttaattta
      301 aataaataaa gtctaatagg tcatattatt atgaataatt gggaatctct taaagaaaaa
      361 gtagataata tagtaattga tttagaagta tcgcacgata acatcgagtc atacccacaa
      421 atagaaaggt tggtatatga taaactgact gaattgaata tagatagaaa agattcttcg
      481 ttatcaaatt acgtaaaatc tacaaaaaaa attatctatt atttagataa atactcttct
      541 tatatcataa gtttcaagca caatgataat aagcaagact atcagttgta ctctaagaac
      601 attaatcaat tattcgcggt agtagcaaat gaatataatg atattaacaa aaagtttagt
      661 aattccagtg gaatagaaaa acttgggcta ggtgattgct tagtggatgc aagcttgtta
      721 tatcttaaat taagtcaaga attgttgcaa ctacctagtc cagaatacag ggcgaaatat
      781 agagaaataa taaaaaccct taaaaacata aaacaattgg atattgattt gttaaagaat
      841 atttatgatt tatctaaagg agaacaaact ttaatcaata aaaactcact aaaggataat
      901 ggacttaagg acataattct agattatcta aaacaaaaga gatatattga tctgacaagt
      961 gaagaaacag gaataaccga aaaaggtcag aaaatcatga agcggcagtc agaaagtagt
     1021 gggaatataa ctattaataa tatatatcag gtatttggtg atatagaaag cgcacagtta
     1081 caaataaata gtacagattc aaagcaagaa ataaagaaat aatttaatgt ataaagatat
     1141 gataatgcaa aaaaaagtac aacgtttaaa atttttagaa aaactttata aattatcaaa
     1201 tggaaaatca aatgtttact ttaatactta ttatacaaaa atagcagagc cactgggttt
     1261 tgatattgaa acagcgacag aaattttcgc atatttaaaa catgaaggtc ttattgcagt
     1321 aactggcggt actaaaattg cattaacaca taaaggattg ttagaatatg aatctgctat
     1381 gactaaccca gaaaaagata cagaacattt tccagctggc ataataaata attatttgaa
     1441 tgtaggtagt aatattaata atatgcaaat tcaaattgga actaaagatt caattcaaac
     1501 tcaatccatc acaattgaga actcaaatga attgattgaa ttattagaag aaattcttaa
     1561 taaaaaagat gattataaat tagatgatgt tgatgaagaa gaattaatag ctgatataac
     1621 aactgctaaa actcaaatag aatcttcaag acctaaaata gaaataataa aaacttctct
     1681 tgattcagtt aagagaattc ttgaatctgc tgttggatcg gcattagcaa atcaaacagt
     1741 tcaaaagctt attcctttta ttgaaagttt aggctaaaat atcacaataa aagaggcaat
     1801 atccattttt gcccttaccc atctcccacc aaagtcttac aaaaaggtca aaaggtcaaa
     1861 agttcagggg ctatacccaa aaaagttgac cttttgacct tttctctcta agttaccgta
     1921 attacagtat ttatctaaac atactgaatt tgtacctact acgcatttcg attgtactca
     1981 atgcgttggg tttgctgata tgtcatttcg ggcagagtga gaaatctttt tcttctgata
     2041 tgggataatc acataaaagg atctctcctt acagtcgaga ttatagtagt ggttaggtta
     2101 ttcatccaag gaaaagtaca agaggtgtat aaacttagat aattcacatt tcgacaggca
     2161 atgcgtttgg ttaactttat ttcgattccc caacaccagc ttattagtga aacatttact
     2221 tttagattca ctaaccctac ttaaacccct ttcccctcgt ataagaagac cttttagtag
     2281 aatacacatc aacttccaga tcggaaaatt cgttgtttgc aaatttctct gctgccagcc
     2341 ttgcaatcat cgcggcattg tcggtgcagt atttaatatg agggataaaa agatcgatgt
     2401 tgttttcgga taacttttcc ttgccaagct ccctgattct tgagtttgcg gctaccccgc
     2461 ctgcaaatgc tactgtacta atattaaact cttcggcagc tcttagtgtt tttactatga
     2521 gtgtttcggc aatcgcttcc tgatagcttg cgcatacatc acataacatc tcatcagagg
     2581 taatttcatt ttttttcaca aagtgcacca gtgcagtctt aagcccgcta aagctaaaat
     2641 caaaactgtc tgtttttaaa aatggcctgg ggaaatcata tttcttttta ttcccatttt
     2701 tggacaattt atcaatttga attcccccgg gataatcaag acctaataac tttgcccctt
     2761 tatcaaaagc ctcccccgcc gcatcgtctc tcgtgtttcc gattaactct aaagacttat
     2821 aggaatttac tttatagaga cttgtatgac cgcctgaaac cacaagtgca atgtatggga
     2881 aggttaattc atgctctatg tttactgaat aagcatgagc ttctatatga tctacggcta
     2941 taagggggac gtccagagaa aatgctaccg attttgccgt ggaaagcccg accatgagtg
     3001 atccgataag tcccggaccg tttgttacgg ctatggcatt aagctcgttt aattttacat
     3061 tagcctcttc aaccgactcg tttattattg gaataatggc ttccacatgt tttctcgagg
     3121 caatctcagg cacaactccg ccgtattttt tatggatatc gacctgtgat gacacaacat
     3181 ttgacaaaat ctcactgccg tttttaatga cagatgctga agtttcatcg catgatgact
     3241 ctattgccag aattaatgaa gacatgaatc tgaattaata attaaattat ctgtattttc
     3301 aatgttactt tttaaaagca aaaggtatac ttaatataca ggccattaaa ttacggatca
     3361 ctaaaaagga tcataaccta tgaagcaaaa aaacagtaaa gacaagatac cacaagctga
     3421 agtaatttcg agagagccct tcccaaattc taataagaca tatgtggaag gacaaaacca
     3481 tgatatcagg gttggaatga gggatattga tgtaacaggc actcttggaa atggctcgga
     3541 tagtgcttac ataaccgtct atgatacaag cggcccgtat accgatcccg aagtagatat
     3601 taatgtacta aaaggtcttg atcctattag ggctggttgg ataaaagaca ggggtgatgt
     3661 ggagcagcta gaggacttta gctcagaatt ttccagaaac tctgcaaaaa atggaaacgg
     3721 tataaccttt aaaaatctaa gaaaaccctt aaaagccaaa aagggcagca atgtaaccca
     3781 gatgcattat gcacgtaagg gaacagttac ccctgagatg gaatatatag ccataaggga
     3841 aaatcagcag atagacaggc taggtgaaaa atataaagaa c
//
LOCUS       DBNGSV010000282        10726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1089410, whole genome shotgun sequence.
ACCESSION   DBNGSV010000282 DBNGSV010000000
VERSION     DBNGSV010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1089410"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1098
                     /locus_tag="AC159U_10435"
     CDS             181..1098
                     /locus_tag="AC159U_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337509.1"
                     /translation="MLRENITFVLIIYILLSTLIAPSSVSSEIHKCVSKEGEIVYTTN
                     PNTVEGCINKTEIPKQKNKDVEINTELEYEANEKDDNDQRYKTILKQETKTDDLVIEN
                     NINSYQNNNDQENLSEDNSLTDKYHLVERLNQSEKQDTYNKVGSNQQRIKHYEKVLEN
                     CLKSAVPTSREIKCGKFRKKLEEEYEKAHYITSEDPLDNISNITSHTKTLEIRLKKCV
                     TTSPPSPSRDYNCWKLEKKIIDITNFTTCRPNTIIDEYTFNCIMASGAEKTIRLIGIG
                     TPELDKFDNGLDALTYSAHLLGMYNYVIL"
     gene            1105..1311
                     /locus_tag="AC159U_10440"
     CDS             1105..1311
                     /locus_tag="AC159U_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR3337510.1"
                     /translation="MQFEDKYQRILAYVYLPNGKMINKLLLDQGLAEVITVPPNVKYS
                     DWLSNDTTSQSDPSIPIIPFNNPY"
     gene            1388..1594
                     /locus_tag="AC159U_10445"
     CDS             1388..1594
                     /locus_tag="AC159U_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337511.1"
                     /translation="MRQKRLRSKMFRAWLNDTEHEFLNSYAEKNFLTASELFRGWLHK
                     VMIKEGYDIQEPQNPETINKGGKY"
     gene            1594..2691
                     /locus_tag="AC159U_10450"
     CDS             1594..2691
                     /locus_tag="AC159U_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3337512.1"
                     /translation="MGVYKKKDRWYIDYYMPSGHRKREVVTIKGVDPAQINRQDALDA
                     LSIRKAEMAQGKFDIAQTKKPVSIEKIINQYLEWAKDNHIAYERDISASKPLLEFFKG
                     KNSSNVTTWSIEKYKSFRKSQDKKAETINKELSFIKSTFKLAVENGVISSNPINGVKL
                     LKVPKSKPRVLKDWEFTKLYQASPEHFKSILLCGYLTGMRRGEIVKLRWEDIDFTDKY
                     IHVNESKNNEYRTIPINDILLYTLVELKKESENEYVFTTKLGKHYTSNTAWKTVWSNT
                     LKNSGIEHCTFHSLRHSFISNLIVNEKEDYITVMNLSGHKTIRMLVRYSHTHEKAKKA
                     AVGKLGKNLELRNFQEHSGLKDISDKEITAV"
     gene            complement(2799..3134)
                     /locus_tag="AC159U_10455"
     CDS             complement(2799..3134)
                     /locus_tag="AC159U_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR3337513.1"
                     /translation="MYRTLLVIGIICSLFFITTPESKAGTGAIPAGACCLEDDSCIDV
                     NGLECAESSGFFVEGVECSEISCPFVPPTDEPSGPTVIVPTMGQWGMILATIILGLFA
                     VLRLRKRTE"
     gene            complement(3531..3608)
                     /locus_tag="AC159U_10460"
     tRNA            complement(3531..3608)
                     /locus_tag="AC159U_10460"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3571..3573),aa:Asp,seq:gtc)
     gene            complement(3626..3700)
                     /locus_tag="AC159U_10465"
     tRNA            complement(3626..3700)
                     /locus_tag="AC159U_10465"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3666..3668),aa:Val,seq:tac)
     gene            complement(3790..6228)
                     /gene="lon"
                     /locus_tag="AC159U_10470"
     CDS             complement(3790..6228)
                     /gene="lon"
                     /locus_tag="AC159U_10470"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3337514.1"
                     /translation="MTDLLKPEDDKDESFQTLPLLPLRDVVIFPYMVAPLFVGREKSI
                     RALEEAMKNDKQIVLAAQKDAKTNDPKKEDIYDIGTIGSIVQMLRLPDGTVKVLVEGK
                     SRTRIRGYVPGSSFFLVEVQDVSTGKNPGAETEALIRTLINSFENYVKLNKKIPSEVL
                     VTVSSMTDPERLSDTIASHLSFKLEDKQEILEIFDPVERLEKLYEKIQSEAEILQVEK
                     KIRSRVKKQMEKAQKDYYLSEQMKAIQKELGDKDDLKSEINEFEEKLKAKSIPEDVEE
                     KIKKEIKKLKMMSPMSAEATVVRNYVDWLLSVPWEEDVTEDRLDIDEATKILDEDHYG
                     LEKPKERIIEYLAVRKLTDKLKGPILCFVGPPGVGKTSLAKSIARSMGRKFIRVSLGG
                     VRDEAEIRGHRRTYIGALPGKIIQGMRKAGTKNPVFLLDEIDKLGMDFRGDPASALLE
                     GLDPEQNKSFNDHYLEVDYDLSNVLFITTANILHTIPWALQDRMEIIHIPGYTELEKL
                     QIAKKFLVPKQREDHGLKPENLTISDSGILSLIRRYTREAGVRTLEREISTLCRKAAK
                     KVVQKGLDHAVKVGEKNIQTILGIPKYKFGEIEENNQIGICTGLAWTEVGGEILTVEV
                     SMVPGKGNFTITGKLGEVMQESAQAAMSYVRSKAEQLGLERYFYQKIDIHIHIPEGAT
                     PKDGPSAGIALVTAIVSALIKKPIDNEVAMTGEITLRGRVLPIGGLKEKILAAHRARV
                     KKVIIPKENEKDLKDIPKNTLKDIEVVLVDNIDEVIKQAIVSDLPVFNDILITPHPAA
                     FPVEPEFGSNVNLS"
     gene            complement(6245..7525)
                     /gene="clpX"
                     /locus_tag="AC159U_10475"
     CDS             complement(6245..7525)
                     /gene="clpX"
                     /locus_tag="AC159U_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3337515.1"
                     /translation="MTTKRDGKDGKLYCSFCGKGQREVRKLIAGPTVYICNECIELCN
                     EIIADEEAKEGYLKQQYSNLPIPSQIKEFLDEYVIGQERAKKILSVAVYNHYKRVELT
                     GQLGNKKNSDVELQKSNILMIGPTGCGKTLLAQTLAKILDVPFTIVDATCYTEAGYVG
                     EDVENMIVSLLQAADYDVERASKGIIYIDEIDKLARKSGDSPSITRDVSGEGVQQALL
                     KIIEGTKANVPPKGGRKHPQQEFIQIDTTNILFICGGAFCGLEDIIGTRIGEKSIGFE
                     SVITNIKEKNLSELLGEFQPEDLIKFGLIPEFIGRIPVTATLDELDESVLMEILTKPK
                     NAVIKQFQRLMELENVELEFTEGAIEEIAHEGIIRKTGARGLRAIIESIMLDVSYEVP
                     TLNNLKRCVITKEVVQGKGNPLFFYEEERKMGSG"
     gene            complement(7522..8121)
                     /gene="clpP"
                     /locus_tag="AC159U_10480"
     CDS             complement(7522..8121)
                     /gene="clpP"
                     /locus_tag="AC159U_10480"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR3337516.1"
                     /translation="MSNFVPIVIEQTSRGERAYDIFSRLLKDRIVFIGSPISDAMADT
                     VIAQLLFLESENPDKDIYVYINSPGGHVTSGLGIYDTMQYIKPDVVTMCMGQAASMAA
                     VLLAAGAKGKRFTLPHSRVMLHQVLGGFEGQATDVDIHAREILRVKQELNEILAKHTG
                     KSIDIIEKDTERDFFLTSKDALDYGVVDAIITRREEAQK"
     gene            complement(8145..9470)
                     /gene="tig"
                     /locus_tag="AC159U_10485"
     CDS             complement(8145..9470)
                     /gene="tig"
                     /locus_tag="AC159U_10485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3337517.1"
                     /translation="MNVDIERVSSTERKLAISIPAKQVDDQFDEIITEFKSTASIKGF
                     RKGKTPVHLIMSMFGKQITAEVTTKVVNDTFYKILEENKLIPVNKPRLEPDELKKGSD
                     FNYTAEFEIVPDFDVSEYEGMELTKEKEKLKKEDTQKALDRLRENAAEAVPLKRARKS
                     KKGDYVFIDQQGFVDGESLPDLKKENLQLLVGEEKLIEDFEKHLIGMKVGEEKEFSIS
                     YDKDFAIKEAAGKEVNFKVKMNDIKERVLPNLDDEFAKQYDLENIKELESRINEDLEK
                     QLEDQADGKMRQEIMKKLIAKYSFDIPQSLLDQEKTHLFNRFANDYQSRGLEVPEITE
                     DVAANIDKRAETNVRASVILGKIAGKEEIYATEKEIDAALYEMAAMYRIPFDQFKQTY
                     EKNNMLDGLESRLTEQKVLDFIIEKAKITEKKAAKSKKKSQENEIDIDS"
     gene            complement(9501..9582)
                     /locus_tag="AC159U_10490"
     tRNA            complement(9501..9582)
                     /locus_tag="AC159U_10490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9546..9548),aa:Leu,seq:tag)
     gene            complement(9592..10263)
                     /locus_tag="AC159U_10495"
     CDS             complement(9592..10263)
                     /locus_tag="AC159U_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3337518.1"
                     /translation="MKLFSAIQYYLSSIPTLLFGVNFYSIPLMFLDGKMLLKTRAGIN
                     FYVKALIEIWTIKEVVLDKQYEDHRRLKEGDIVIDIGAAIGEFSIYAAKTAKTVYGFE
                     IVDESIERMRGNMEINNCTNINLIQKRVDSLNDIFSEHNIEHCDFLKVDCEGCEYAIF
                     KDVSDEVLDKIDYMAFEVHFFNDDMIKAYKELTARLSQKFSLIETPSPVHSNIAFLYA
                     IRKNT"
     gene            complement(10320..10393)
                     /locus_tag="AC159U_10500"
     tRNA            complement(10320..10393)
                     /locus_tag="AC159U_10500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10357..10359),aa:Lys,seq:ctt)
     gene            10560..>10726
                     /locus_tag="AC159U_10505"
     CDS             10560..>10726
                     /locus_tag="AC159U_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3337519.1"
                     /translation="MVEPKSKSNKRQRADKIKAYRCKNLIAVIEDPHDMKNIGTVIRN
                     VNALGVEKAYIV"
BASE COUNT         3272 a         2170 c         1784 g         3500 t
ORIGIN      
        1 tcttaaatat cttattatct gctctggtgt gtaacgcttt ctgggcattt cccttgcctc
       61 ctattcatag ggtttattac ctaaaattct aacttataat ctggtactat tatatggggc
      121 aaggtcagtc aagagaatta tattaaagca attgatgata tagtataatg ttaattgtct
      181 atgcttaggg aaaacattac atttgtttta atcatttata tcttattatc gactctaatt
      241 gctcctagct cagtatcaag tgaaattcat aagtgtgtat caaaagaagg tgaaatagtt
      301 tatacaacca atcctaatac agttgaaggg tgtatcaata aaacagaaat cccaaaacaa
      361 aagaataaag atgttgaaat aaatacagaa ttagaatatg aagcgaatga aaaagatgat
      421 aatgatcaaa gatacaaaac aatactcaaa caagaaacta aaacagatga tcttgtaata
      481 gagaataata taaattcata tcagaataat aatgatcagg aaaacctatc cgaggacaac
      541 tctcttactg ataaatatca cttagtagaa agattaaatc aatctgagaa acaggatact
      601 tataacaaag ttggatcaaa tcaacaacga attaaacatt atgaaaaggt actagaaaat
      661 tgtcttaaaa gtgcagtacc aacaagcaga gaaattaaat gtggtaaatt cagaaaaaaa
      721 cttgaagaag aatacgagaa agcccattac ataacctcag aagatccact agacaatatt
      781 tctaatatta cttcacatac taaaacactt gaaataaggc ttaaaaaatg tgtaacaaca
      841 tctccgccaa gtccaagcag agattataat tgttggaaac tggaaaaaaa aataatagac
      901 ataacaaatt ttactacttg tagaccaaat acaattatag acgaatatac attcaattgt
      961 attatggcat ctggggcaga aaagactata agacttatag gtataggtac acctgaatta
     1021 gataagtttg ataatggtct agatgcccta acttactcag ctcatttact tggtatgtat
     1081 aattatgtaa ttttataatt tgatattcaa tttgaagata aatatcaaag aattcttgca
     1141 tatgtgtatc ttcctaacgg caagatgata aataaattat tactagatca aggcctagca
     1201 gaagtaatta ctgtacctcc aaatgttaaa tattctgatt ggttatccaa tgatactaca
     1261 tctcaatccg atccatcaat accaataatt ccatttaata acccctatta aacaacaaat
     1321 agttctataa aattgaatta tcattaaata tttgacttat gaagtcatgt agctatatta
     1381 ttactacatg agacagaaaa gattaagatc aaagatgttt agagcatggc taaatgatac
     1441 agaacatgaa tttttaaata gttatgcaga aaagaatttt ctcacagctt ccgaactatt
     1501 caggggctgg ctacacaaag taatgattaa ggagggatat gatattcaag aacctcaaaa
     1561 tcctgaaaca atcaacaaag ggggtaagta ttaatgggag tatataaaaa gaaagaccgc
     1621 tggtatatag attactatat gccaagtgga catagaaaaa gagaagttgt aaccattaag
     1681 ggtgttgacc ctgctcagat aaataggcaa gacgctttgg acgctctctc aataagaaaa
     1741 gcagaaatgg cacagggtaa gttcgatatc gcacaaacaa aaaaaccagt atcaattgaa
     1801 aaaataatta atcaatattt agaatgggct aaggacaacc atatagccta tgagcgtgat
     1861 ataagcgcat ctaagccctt attagaattc tttaagggta agaactcatc caatgttact
     1921 acatggtcta ttgagaaata taaatcattt agaaagtctc aagataagaa agcggaaact
     1981 ataaataagg aattatcttt tataaagagt acttttaaat tagccgttga gaatggggta
     2041 ataagttcca atcccattaa tggggtaaag ctcttaaaag taccaaaatc aaagcctagg
     2101 gtactaaaag attgggaatt tactaaactt tatcaagcat ccccggaaca tttcaaatcc
     2161 atactgctct gtgggtattt aacaggaatg aggcgtggtg agattgttaa actcagatgg
     2221 gaagatatag acttcactga taaatatata cacgttaatg aatccaaaaa taatgaatac
     2281 cggacaatac caataaacga tattctttta tatacacttg ttgaattaaa gaaagaatca
     2341 gaaaacgaat atgtatttac aaccaaacta ggaaagcact acacaagtaa tacagcttgg
     2401 aaaactgtat ggtctaacac tctcaaaaat tcgggcatcg agcactgcac ttttcactca
     2461 ctaagacatt cttttatttc taatcttata gtaaatgaaa aggaagatta tataacagtt
     2521 atgaacttat ccgggcataa aacaatcagg atgcttgtaa ggtattctca tacacatgag
     2581 aaagcaaaga aggctgcggt tggcaagtta gggaagaacc ttgaattaag aaattttcaa
     2641 gaacatagcg gtcttaaaga tatttcagat aaagagatta cagcagtttg atattgcttt
     2701 tacttaattt atagagatag ttaaaaccgt ttctataaaa accccacaac caaaggggtt
     2761 aagctctgat tgtgggttca gtgattattt taattttatt attctgttct ttttctaagt
     2821 cttaacacgg caaacaaccc caaaattatt gttgccagta tcattcccca ttgtcccata
     2881 gttggaacta taacagttgg acctgaaggt tcatcagttg gaggcacaaa gggacatgat
     2941 atttcactac attccactcc ttctacaaaa aatccagaag actctgcaca ttcaagaccg
     3001 tttacatcta tacatgaatc atcttccaag cagcatgctc ctgcaggaat tgcacctgtt
     3061 cctgctttag attccggagt cgttataaaa aacaaggaac aaataatgcc tataactaat
     3121 aatgttctat acataatatc ctttaccctc caaatgattt attaagttga tacttgaata
     3181 tcacatccag caaatcaaat caagcaaaaa tttattatat aacattaaac tgcattaaca
     3241 tataaatatc aagctttttt taactcaact gttaatttaa gtgcaatatt gtagtgctta
     3301 aaatagtagt gacaccgatc ttaaaacctt atcttctcca cctccctgta cttatgctcc
     3361 tcagatacat ggctgtaggt ttttatatgt actgaaaatg ataggaatta ttacggattt
     3421 attgaaagaa attaagatta tagacaccta tttggacacc aaggcagata tatgattaaa
     3481 atatggttac gcaagaacgc tgcaacttat tgatattatt aggagaataa tggcggggcc
     3541 gacgggaatt gaacccgcga cctctggctt gacaggccag cgttctaacc ggtctgaact
     3601 acggccccgc attaattgtc tttagtggtg ggcggtacag gatttgaacc tgtgaccccc
     3661 tgcttgtaag gcaggtgttc tcccgctgaa ctaaccgccc tctcaaatac aagctgaaaa
     3721 aacgagtgta gtttataact gaaataagct tatttgtcta gtgtaatttt aacaaaatat
     3781 ccggcaaatc tatgaaagat tcacattaga gccaaattcc ggctcaacgg gaaaggcggc
     3841 tggatgtggc gtgatcaaaa tatcattaaa tacaggcaga tcagacacta tggcttgttt
     3901 aataacttca tcaatattat ccactaatac tacttcaata tccttgagtg tgtttttagg
     3961 tatgtcctta agatcttttt cattttcttt tggaatgatt acctttttca cccttgccct
     4021 gtgagccgca agtatctttt cttttagccc tcctatggga agcacccttc ccctaagtgt
     4081 tatttctcca gtcattgcta cttcattatc aatcggtttt ttaattagcg ctgaaacaat
     4141 tgctgttaca agtgctatac ctgccgatgg tccgtccttt ggcgtagcgc cttcaggtat
     4201 atggatatgt atatcaatct tctgatagaa ataacgctca agaccaagct gctcagcttt
     4261 tgagcgcaca tagctcatag ccgcctgagc ggattcctgc ataacttctc caagcttccc
     4321 cgtgatagta aaattgcctt tacctggaac cattgatact tccacggtaa gaatttctcc
     4381 tcctacctcc gtccaggcaa ggcccgtaca aatacctatc tgattatttt cttctatttc
     4441 gccaaacttg tattttggta tcccaagtat ggtctgaata ttcttttcac caacttttac
     4501 ggcatgatca agtcccttct gcacgacctt ctttgcagcc tttctgcaca aagttgatat
     4561 ttccctctcg agcgtcctaa ctcccgcttc tctggtatac cttctaatga gtgacaatat
     4621 tccactgtca gaaatagtta agttttcagg ttttagtccg tgatcttcac gctgtttggg
     4681 aactaggaat ttttttgcaa tctgaagttt ctctaactct gtatatcccg ggatatgtat
     4741 tatttccatc ctgtcctgta gtgcccaggg aatagtatgt agtatatttg cagttgtaat
     4801 aaacagcaca tttgacaggt cataatcgac ctccaggtaa tgatcattaa aagatttatt
     4861 ttgctcggga tcaagccctt caagtaacgc cgatgccgga tcacccctga aatccatccc
     4921 aagtttgtct atttcatcga gtaaaaacac cgggtttttt gtacctgctt ttctcatgcc
     4981 ctgaataatt tttccgggaa gagcgcctat atatgttctc ctatgacctc ttatctcagc
     5041 ctcatctctc accccgccaa gagatactct gatgaatttc ctccccatag accttgctat
     5101 agattttgca agtgaagtct tacctacccc cgggggaccg acgaaacata gtatgggccc
     5161 ttttagtttg tccgtgagtt ttcttacggc aagatattct attattctct cttttggctt
     5221 ttcaaggcca taatgatctt catcaagtat ctttgtagct tcatcaatat ccagcctgtc
     5281 ctctgtaaca tcttcttccc acggaaccga tagcagccaa tccacgtagt ttctaaccac
     5341 cgtggcctca gcagacatag gagacatcat ttttagcttt ttaatctctt tttttatctt
     5401 ttcttctaca tcttctggaa tacttttagc tttaagcttc tcttcaaact catttatttc
     5461 agacttaaga tcgtccttat cgccaagctc cttttgtatg gctttcatct gctcagataa
     5521 ataatagtcc ttctgagcct tttccatctg ttttttaact ctcgatctta tctttttttc
     5581 tacctgtaaa atttcggctt ccgattgaat tttttcataa agcttttcaa gtctttcaac
     5641 aggatcaaag atttctagta tttcctgctt atcttcaagt ttaaaagaaa gatgagatgc
     5701 aatggtatcg gaaagcctct caggatctgt cattgaagat acagtgacaa gcacctctga
     5761 ggggattttc ttattaagct tcacatagtt ttcaaatgaa ttaataagtg ttcggatcaa
     5821 agcctcagtt tctgcgccag gatttttgcc agtggataca tcctgtactt ctacaaggaa
     5881 aaaacttgat cccggaacat atcctctgat tcttgtcctc gacttaccct ctaccaacac
     5941 tttaacagta ccgtcgggaa gcctcagcat ctgaactata gagcctatag ttccaatatc
     6001 gtatatatct tcttttttgg gatcattggt cttggcatcc ttttgagcag ctaaaactat
     6061 ttgcttatca tttttcattg cctcttcgag agcacgaatg gatttttcgc gtcccacgaa
     6121 tagcggcgct accatgtaag gaaatattac tacatcacga aggggaagaa gaggaagcgt
     6181 ttgaaaactt tcatctttat catcctcagg ttttaataga tcggtcataa aagctcctta
     6241 aaaatcaccc cgagcccatc tttctttctt cctcatagaa gaacagagga ttgcctttac
     6301 cctgcacaac ctcttttgtt ataacgcatc gtttgagatt attgagcgtg ggtacttcat
     6361 aagaaacatc gagcataatt gattcaatta tcgcccttag tccccttgca cctgtttttc
     6421 tgatgatccc ttcatgtgct atttcttcaa tagcaccttc ggtaaactca agctctacat
     6481 tttcaagctc catgagtctt tggaactgtt taataacagc attctttggt tttgtaagaa
     6541 tttccataag tactgattca tcgagctcat ctagcgtggc tgttacagga atacgaccta
     6601 taaattcagg aatgaggcca aacttgataa gatcctcagg ctgaaattcc cctaataatt
     6661 cacttaggtt cttttctttt atatttgtta ttacagattc aaatccaatt gatttctcac
     6721 ctattctggt acctataata tcttccagac cgcaaaaagc accgccgcat ataaaaagta
     6781 tattagttgt atcaatctgt atgaattcct gctgtggatg cttacgtccg ccctttggtg
     6841 gaacatttgc cttggtacct tcaataattt taagaagtgc ctgctgtacc ccttcaccag
     6901 aaacgtctct tgtgatagag gggctgtcac ccgattttct tgcaagttta tcaatttcat
     6961 caatgtaaat tattcccttc gatgcgcgtt caacatcata gtcagcagcc tgtagaaggc
     7021 ttactatcat attttctaca tcttcaccaa catatccagc ttctgtgtaa catgtggcat
     7081 ccactattgt aaagggaacg tcgagaatct ttgcaagagt ttgagcaagc agggttttac
     7141 cacaccctgt agggccaatc atgagaatat tacttttttg aagctctaca tcgctgtttt
     7201 tcttgtttcc taactgccca gtgagttcaa cccttttgta gtgattgtat acggctacag
     7261 aaagtatttt ctttgccctt tcctgtccaa ttacatattc atccaagaat tctttgatct
     7321 gtgatggtat tggaagattg gaatactgct gttttagata accttcctta gcctcttcat
     7381 cggcaattat ctcattacaa agctcaatac attcattgca tatgtataca gtgggtcctg
     7441 caatgagctt acgtacctct ctttgcccct ttccacaaaa ggagcaatat agctttccgt
     7501 cttttccgtc tcgtttagtt gtcacttttg tgcttcctcc cttcttgtta ttatagcatc
     7561 aactacgcca taatctaagg catcttttga agttaggaaa aaatctctct ccgtatcttt
     7621 ttctattata tctatacttt tgccggtatg ttttgccaaa atttcattta gttcctgttt
     7681 tacccttaat atttcccttg catgaatgtc tacgtcagtt gcctgacctt caaatccacc
     7741 aagcacctga tgtagcataa cccgtgaatg cggtaaagta aatctctttc ccttggctcc
     7801 cgctgccaaa agtactgctg ccatgctagc agcctgcccc atacacattg ttacaacatc
     7861 gggctttatg tactgcatgg tatcgtaaat cccaagcccc gaggtcacat gcccgcccgg
     7921 ggaatttata tatacgtaaa tatctttatc agggttttct gattccagga ataaaagctg
     7981 tgctatcaca gtatctgcca tagcatccga tatgggagaa cctatgaata caatcctgtc
     8041 ctttagaagt ctggaaaaga tatcatatgc cctctcaccg cggctggtct gctctataac
     8101 tatggggaca aaattagaca tgaatagact caagtatact aattctatga atctatgtca
     8161 atttcatttt cttgggattt tttcttgctt tttgcagctt ttttctcagt gattttggcc
     8221 ttttcgataa taaagtctaa aaccttctgt tctgtgagtc ttgattcaag gccatcaagc
     8281 atattatttt tctcatatgt ctgtttaaac tgatcaaaag gtatcctgta catagcggcc
     8341 atttcgtata gtgcagcatc aatctctttt tctgttgcat agatttcttc tttacctgca
     8401 atctttccaa gtattacaga agccctgaca ttagtttcgg cacgcttgtc tatattggcg
     8461 gcaacatcct cagttatttc aggcacttcc aaacctctgc tctgatagtc attagcaaac
     8521 cggttaaaca gatgtgtctt ttcctgatca agaaggctct gtggaatatc aaaagagtat
     8581 ttagctatta gtttcttcat tatttcctgt ctcatttttc catcggcctg atcctcaagc
     8641 tgtttttcga ggtcttcatt tatcctgctc tcgagttctt tgatattttc taaatcatac
     8701 tgctttgcaa attcatcatc gagattggga agcacccttt ctttgatatc attcattttt
     8761 actttaaagt taacttcctt tccggcagct tcttttatcg caaaatcctt atcataggaa
     8821 atagaaaact ccttttcttc gcctactttc atcccaatca ggtgtttttc aaaatcttca
     8881 atcagcttct cttcaccaac caaaagctgc aggttttctt ttttaaggtc gggcagagat
     8941 tctccatcta caaatccttg ctgatcaatg aacacataat cacctttttt ggattttctt
     9001 gcccttttta acggtacggc ttcagccgca ttttctctaa gcctgtcgag agctttttga
     9061 gtgtcttcct ttttcagttt ttccttttct tttgttaact ccattccctc atattcactt
     9121 acatcaaaat caggaactat ctcaaactct gccgtgtaat taaaatcaga gcctttttta
     9181 agctcgtcag gctcaaggcg gggtttattt actggaataa gtttattttc ttcaagtatt
     9241 ttatagaaag tatcgtttac aaccttggta gttacttctg ccgttatctg ttttccaaac
     9301 atcgacatta ttagatgaac aggagtctta cctttcctaa acccttttat actggctgta
     9361 cttttaaatt ctgtaatgat ttcatcaaac tgatcgtcta cctgctttgc aggaatagaa
     9421 atcgctagtt ttctttctgt actgcttact ctttcaatat ctacgttcat tttatctgta
     9481 acccctgttt agaagattaa tgcgagaggg gggagtcgaa cccccacacc tcgcggtact
     9541 ggatcctaaa tccagcgcgt ctgccagttc cgccactctc gctaaaggca attaagtatt
     9601 tttcctgata gcatagagaa atgcaatatt tgaatgcacg gggcttggag tttctatgag
     9661 actaaacttt tgagaaagcc ttgcggtaag ctctttatat gcctttatca tatcatcatt
     9721 aaaaaagtga acctcaaacg ccatataatc aattttatcc agtacctcat cagatacatc
     9781 tttaaatatt gcatattcac acccctcaca gtccactttt aaaaaatcac agtgctctat
     9841 attgtgttca gaaaagatgt catttagtga atccaccctt ttttgaatga gatttatatt
     9901 tgtacaatta tttatttcca tattgccacg cattctctca atactctcat ccactatctc
     9961 aaaaccatat actgtctttg ctgtttttgc tgcatagata ctgaactcac ctatagctgc
    10021 accaatgtct attactatat ctccctcttt taatctcctg tgatcttcat actgtttgtc
    10081 gagcaccact tcttttattg tccagatttc tataagtgcc tttacataaa aatttatgcc
    10141 ggctctggtt ttcaaaagca tctttccgtc taaaaacatt agtggaatag agtagaaatt
    10201 aaccccgaaa agcagggttg ggatagatga aagataatat tgtatggctg agaataattt
    10261 catagaaacg ggtacttagt acttctaaaa ttaaatgatc tgaataatgt ataaattttt
    10321 gggctgtggg ggaatcgaac cctcgaccca gtgattaaga gtcacttgct ctgcctagct
    10381 gagctaacag cccaaaaaaa tcaattattt tttagcttag ctgaaaatgt ttggggtgac
    10441 aatgggtagg tacagatgat atattttaat tctagtagaa atagagattt gttattttga
    10501 taatattaaa tctcagaaaa aatatatatt aaacctaagg cacttacgga gagcaactag
    10561 tggttgaacc gaaatcaaaa agtaacaaaa ggcaacgtgc tgataaaata aaggcgtatc
    10621 gatgcaagaa cctgatagca gttatagaag atccgcacga tatgaaaaat attggaactg
    10681 tgataagaaa tgtaaatgca ctgggtgttg agaaggctta tattgt
//
LOCUS       DBNGSV010000283         3458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1020935, whole genome shotgun sequence.
ACCESSION   DBNGSV010000283 DBNGSV010000000
VERSION     DBNGSV010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1020935"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..348)
                     /locus_tag="AC159U_10510"
     CDS             complement(79..348)
                     /locus_tag="AC159U_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337520.1"
                     /translation="MLVGVGVGVFVGVGVGVFVGVGVGEGVPVGLGVGVGVLVGVGVG
                     VFVGVGVFVGVGVGVLVGVGVGVFVGVGVACQAPEFRSSFKVRIC"
     gene            569..793
                     /locus_tag="AC159U_10515"
     CDS             569..793
                     /locus_tag="AC159U_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR3337521.1"
                     /translation="MCSVTTEEECIGFYQGDFSICEINTCDDPVTPPPPPSEGPTVII
                     PTLSEWGMIIATILLGFAAVLRFRKRDTEI"
     gene            complement(886..2544)
                     /locus_tag="AC159U_10520"
     CDS             complement(886..2544)
                     /locus_tag="AC159U_10520"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3337522.1"
                     /translation="MNKFTTPVKKIPVLRQSYGYKTISEMTANSAEIYSDKTAYEIPR
                     DNQIIQYTFGDVINLVQKLAKHLSETGLSRGDRIAIIGENSPHWAISFFAVSWVGAVA
                     VPLDARSSVDNHKLALSHSEASCIITSKSFFSSIEALKNSIPSLKTVIRMEEIDELNN
                     KYSTGISPANIGGDDLMEILFTSGTTGDPKGVMLSHKNLMSNVEDVRRIIDLNEDDKA
                     FSILPIHHVYECTGGLLSTFYNGVDVFYARSLKPREMLADLKTASPSIWLNSPLILEK
                     LYQRINKELSTQTGVKSIIANMMPKHIIGKKVKQKLGLSNIRLILSGGAALADWVGEG
                     LDELGFPILQGYGLSESSPLISVNPPSNPKNDSVGMVIPSVEAKIVDIDSEGNGEIWA
                     KGDNIMKGYYKNDSATKEVLTGDGWLKTGDIGYFDEDGYLYITGRKKFVIVTKGGKNV
                     FPEEVEEKLCKSPFIEEAMVFSPDDSQIQAIIYPNYDELKEKLGEDFDPSICKDFIQT
                     KIREANKDLEAYKKVSKFELREEEFPKTTTRKIKRHLFKDYKIN"
     gene            2598..3299
                     /locus_tag="AC159U_10525"
     CDS             2598..3299
                     /locus_tag="AC159U_10525"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337523.1"
                     /translation="MKKYFQFHRARNFIITVLLISSVIGSCAGLLTGSSAIVEFERGL
                     SYFNRGEFDKATTHFIRATELDPNYTKAYIYLGSSYINLKQWYSALIPLRTAYKISPA
                     QTRDEVSNLLIDTLFALVVGEIKKGNFDASKEYLAEYLDLNSSAGNSTNQIVSFLVSF
                     AAEELKNGNLDNTYSALKLALQIDPKNSDAYIGLGRAYFKDGRIFDALDNMRKAILLD
                     PNNKEARDLIQQYNN"
     gene            complement(3307..>3458)
                     /locus_tag="AC159U_10530"
     CDS             complement(3307..>3458)
                     /locus_tag="AC159U_10530"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR3337524.1"
                     /translation="TSKYGCNAAMVISKNYFTEDALEFSKSSPSELIDRNAFGKWIKD
                     TKLCS"
BASE COUNT         1027 a          757 c          603 g         1071 t
ORIGIN      
        1 ctgctccaaa agctttaata caagacaaaa aaaccacgaa ctatgaacaa actttcactc
       61 caactcactg acaaaatgtt aacaaattct aactttaaaa ctggacctaa attctggggc
      121 ttgacaagct actccaacac caacaaatac acctacacct actccgacta atacgccaac
      181 gcctactcca acaaatacac ctacacctac aaatactcct acgccaacac caaccaacac
      241 cccgaccccg acgccaagtc caactggtac tccttcacca acaccgactc ctacaaatac
      301 tcctacacct acaccgacaa atacaccgac gccaacacct accaacactc ctacaccaac
      361 gcctacacct acaggaacac catcaccaac accgactcct acaaatactc cgacaccgac
      421 tggtactccg acacctacac cgacgggtac acttactcct acaccaactc cgacaccgac
      481 aggtactcca acacctacac cgacaggtac acttactcca gctccgacac caacaccccc
      541 tgtaacagga gcatgctgtg atctggattt gtgcagcgtt actactgaag aggaatgtat
      601 agggttctat cagggagatt tctcaatttg tgaaataaac acatgcgatg atcctgtgac
      661 accgccacca ccaccttcgg aaggtccaac agtgataatt cctactctta gtgaatgggg
      721 aatgataatc gcaactattc tacttggttt tgcagctgtt cttaggttta gaaaaagaga
      781 tacagagatt taatagataa tatcaattgt tataaaaaaa ttagagggcc actgatattt
      841 tgggtatcgg tggccttttt tatataagaa taaaacaggc atatattaat ttattttgta
      901 atccttaaat aagtgtcttt ttatttttct tgtagtagtt ttagggaatt cttcttctct
      961 taattcaaat ttgcttactt ttttatatgc ttctaaatct ttattggctt ctctaatttt
     1021 agtttgaata aaatctttgc atatgcttgg atcaaaatcc tcacccagct tttcttttag
     1081 ttcatcatag tttggataaa ttattgcctg tatctgtgag tcatcagggc tgaacaccat
     1141 tgcttcttct atgaaaggtg atttgcacag cttttcttcg acctcttcag gaaagacgtt
     1201 cttgccgccc tttgtaacta tcacaaactt ctttctgcca gtaatgtaga gatacccatc
     1261 ttcatcaaaa tatcctatat cacccgtttt aagccagccg tcaccggtta atacttcttt
     1321 tgtagccgaa tcatttttgt agtatccttt catgatattg tcacccttgg cccaaatctc
     1381 gccgttaccc tcagaatcta tatctactat ctttgcttcc acgcttggta ttaccatgcc
     1441 tacactatcg ttttttgggt tgcttggagg atttacgctt atgaggggag atgactctga
     1501 gaggccgtaa ccctgaagaa ttggaaatcc aagctcatcc aatccctctc cgacccagtc
     1561 ggcaagggca gctcctccgc ttaaaataag tcttatgttg ctaagaccaa gcttctgctt
     1621 aacttttttg ccgattatgt gtttgggcat catattggct attatggatt taactcctgt
     1681 ctgtgtgctt agctccttgt ttattcgctg ataaagtttt tcaagtataa gcggtgagtt
     1741 cagccatata gacgggcttg ctgttttgag gtcagccagc atttcacgtg gttttagact
     1801 ccttgcataa aacacatcta ctccgttata aaaagtggat agcagacccc ctgtgcattc
     1861 gtatacatgg tgaattggaa ggattgaaaa tgccttgtcg tcttcattaa gatcaataat
     1921 cctgcgtaca tcttcaacat ttgacataag gtttttatgg cttagcatta ccccttttgg
     1981 atcgcccgta gtgcctgaag taaaaagtat ttccatcagg tcatcgcctc cgatattagc
     2041 agggcttatg ccggttgaat atttgttgtt aagttcatca atttcttcca ttcttattac
     2101 agttttaagt gaaggaatgg aattttttag agcttctatg gaactaaaaa aactttttga
     2161 tgtaattata caagaagcct cggaatggct taaggcaagc ttatgattat ccacagatga
     2221 tctggcatcg agcggaactg ctactgcacc tacccatgat acggcaaaga aagaaattgc
     2281 ccaatgcggg ctgttctctc cgatgattgc tatcctgtca cctcttgata atcctgtttc
     2341 gcttagatgt tttgcaagtt tttggactaa atttattaca tcaccaaagg tgtattgaat
     2401 aatctggtta tcacggggaa tctcatatgc cgttttatcg ctataaatct cagcagagtt
     2461 tgcagtcatt tcagaaatag tcttatatcc ataagactgc ctgaggacag gtattttctt
     2521 taccggtgtt gtaaacttat tcatatacgc taataataaa tcaaattatc gaatgaataa
     2581 aataaatagg ggttaaaatg aaaaaatatt ttcagtttca tagagcaagg aattttatta
     2641 ttaccgtatt attaatatcc agcgttatcg gttcttgtgc aggacttctt actggaagct
     2701 cagctattgt tgagtttgaa agggggctta gttattttaa cagaggggag tttgataaag
     2761 ctacaactca ttttataaga gctacagagc ttgatcccaa ctataccaaa gcatatatat
     2821 accttggaag ctcttatatt aatcttaaac agtggtacag tgcactcata ccgcttagga
     2881 ctgcttataa gatatcacct gcacaaacca gggatgaggt gtcaaatcta ttgattgata
     2941 cgctgtttgc actggtagtt ggtgaaatta aaaaaggaaa ttttgacgct tctaaagaat
     3001 atttagcaga atatctcgat ctgaacagct cggccggtaa ttctacaaac cagattgtat
     3061 cgtttcttgt gtcctttgca gcagaagagc ttaaaaatgg taatttagat aatacatact
     3121 ctgctcttaa actggcactt caaattgatc caaagaactc cgatgcttat atagggctag
     3181 gtagggctta ttttaaagac ggcaggatat ttgatgcact agataatatg agaaaggcaa
     3241 tactgcttga tccaaacaat aaagaggctc gggacttaat tcagcagtat aacaattagg
     3301 tgtttactaa ctacagagct tagtatcttt gatccatttg ccaaaagcgt ttctgtctat
     3361 aagctcacta ggggatgact ttgaaaattc taatgcatct tcagtaaaat agttttttga
     3421 aataaccatg gcagcattac atccatactt ggatgtat
//
LOCUS       DBNGSV010000284        13236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_743403, whole genome shotgun sequence.
ACCESSION   DBNGSV010000284 DBNGSV010000000
VERSION     DBNGSV010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_743403"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC159U_10535"
     CDS             <1..259
                     /locus_tag="AC159U_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337525.1"
                     /translation="GATAICVDNKAGYCFQFANELLTSDVSFVLQADFGWKGSPAATN
                     VSGRKDFAIYSDGSIRCSTSLVSSRNPGAFEANRNSLACDD"
     gene            complement(256..1275)
                     /locus_tag="AC159U_10540"
     CDS             complement(256..1275)
                     /locus_tag="AC159U_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3337526.1"
                     /translation="MILNSSATQKNKLILTFLILLALWTVVVLISISSGTFKLELLSN
                     FDSLSEIDKKVFFGIRLPRILLASIAGGTLAVCGAALQAMFRNPLASPFTLGVSGGAS
                     LGALIAIKLGFAYSILGFSLISVFSFIFSILTILFVYSVSKVNGVVATGRLLLAGVVM
                     NFLFSAFILFIQFFSKFTESLHTLKWIMGSLDIIGFSLVWKTALLAVPACLLLISMTK
                     DMNIYSLGNDVASSLGVNIKKMELKIYFATSLAAAAVISVTGPIGFVGLFIPHILRMI
                     IGVDNRILLPCSFLLGASFLTITDTISRTIISPVEIPVGIVTAFIGGIFFLWLLVKSK
                     KEVII"
     gene            complement(1298..2035)
                     /locus_tag="AC159U_10545"
     CDS             complement(1298..2035)
                     /locus_tag="AC159U_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337527.1"
                     /translation="MKNPVLSNIEISIKRAEITYILGANGAGKSTLLKVLCGILDPDS
                     GYVLYEDTDIKTIGRAALAKQIAYVSQETTFGFPFTVKEVVLLGRSPYIGRYEFERDS
                     DFEIAKSAMEQVGITHLQDRNINNISGGERQLVSLARAIAQQPQVMVLDEPGTFLDLK
                     HKSMIFSIIKKLCTDEGITIIVATHDISSIINTLGISILLKNGKVLYSGNSSDVLTEH
                     NLTEVYDTDVKILRDNSSLLITANTLN"
     gene            complement(2080..2949)
                     /locus_tag="AC159U_10550"
     CDS             complement(2080..2949)
                     /locus_tag="AC159U_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3337528.1"
                     /translation="MNYIFRPIICFIFVFFFIPTTFLYSESKYPDRVISLSPSITEII
                     YALDAWNKVVGVTMYSDFPPEVKDIQNVGGWVNPNLEMIIQLQPDLVIMLTDQDNIFG
                     DKLRSLGLSTLSVDSNPSIAHINESIITIGKALGKQDESIRLKEQIESEVTTIKEILS
                     KQEPKTVLCVIGRNPGTLEDIYVVGNTSYINELITVSGGVNVIEKKKTALKISKEAIF
                     SLDPDIIIEINHDKTDKEKEVKQIWSALSEARAIKNDQLSVISSSAILHPSQRIIAGL
                     KELINIIHPQLAH"
     gene            complement(2951..3895)
                     /locus_tag="AC159U_10555"
     CDS             complement(2951..3895)
                     /locus_tag="AC159U_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR3337529.1"
                     /translation="MRICSFLPSATEILYELGLGENLTGITHECDYPEDVSNKLRVIN
                     SFLKPEELNSKDIDDLVAKNKEEGKSTYIIELEKLKEASPDIIITQGLCEVCAVSGNE
                     VVNAAKALGSMPEIVSLEPKTVGEVLDSFITVGKATNKENEAKELHDKLSKRLDTIKQ
                     KLEIEDERPKVFALEWFDPPYVGGHWVPEMVELAGGNPVLGRKGKPSLKVTWDDVLKS
                     TPRYTIVMPCGYDIDKSLDEMDSLLSVDQWHKLPSTRKGHAYIVDANSYFSRPGPRIV
                     TGIEILAHILHPDLFGPDFPADSFINYRNYIYLQDFLG"
     gene            complement(3966..5507)
                     /locus_tag="AC159U_10560"
     CDS             complement(3966..5507)
                     /locus_tag="AC159U_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3337530.1"
                     /translation="MKNILCLSLALIASVYFIACGGSDDEFDPDKFFALNNGPVMILN
                     SELNQNVDPNTTVVVGGLDGAVNSNAFVSITNLDTTEQRVVRANSRGGFIAALTGSVF
                     DEFEIVSGGKSIIVNSQVFIDIPLDTRIKLMNGAVNRNLAQLGSVPTNIKIFNGRAYV
                     LNGFSDNIQVFDITQNPPVQTGTIVLPLGSDPVGIAFFNDEHAYVTNLISQTVAIVNI
                     QDMTCETIISQDEGNFTPCDEVIIVDEGTFANPAGIVIANNKAYVANQNIDNSTFAPM
                     GKGFITVIDSETNEIDTVIDATGEGTSGTGKGIMLFNDKVYVVNSGNVLFDFDTGLFS
                     CDTESPISIDVINTASDTVEDSIEFPLSEINPTVCSPESISASPDGDFAYIGSQISGV
                     LFKINLNTNDLIRGSGDPIIITDTEGLDATFDIEFSSSGYGFVAMFNSDTVFVFDPEI
                     DDISPFPFLGEFPVGLRGDNPSSDFFDGPQVMAITDNGQNPDLFFITGISEQLGSINT
                     SLFIP"
     gene            complement(5519..7393)
                     /locus_tag="AC159U_10565"
     CDS             complement(5519..7393)
                     /locus_tag="AC159U_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3337531.1"
                     /translation="MKFFITLLILLIQTGLVRAQDAGQELEPVIVEDTYLRPPLDYPS
                     AFSTVIDLDAYLGEFNTATELLNLSPGVVVRDFGGLGQLKTLSIRGSSNDQVVVLLDG
                     VRLSSPIGGGIDLSTIPIDYIESIEVVRGGGSALAGSDAIGGVVNLIPRKTGDKYFST
                     SATYGSFDTFKANLSGAGSFGELGYFFSYTHAQSEGDFEFESINGFDVKRINNEFLSE
                     SFYSKLDFELGDWNLSLFNDFYYDDKGIPGLGEFQEPNSNQQDIRNLTSLKIAKDGLF
                     TPSLNFETIIFNRFDELEFKNPEPVLGLPIDTLSQNYSFGFNTKFTWFAPKEHTITFA
                     TELKADVLQDDDFKNPERLTFSSYLGDEFSFFYNFITLHPLVRLDVFKTFADENDTEA
                     QVSPKLGLMVSPFNWIALKANVGRSFRAPNFGELFFPEQGFIGGNPNLESETSIDFDI
                     GLVVNRPRFAFEVNYFKNNIDDLILFVFVSAQRIEPRNVGDVDQQGLEASLRVRPTDF
                     LELYSAYTLLDGTLDDTGTQLPGRPKHKFDFRGDLDIKYASLFWESHFVDEIPLSAFP
                     NARRTENRYTHDTGAKSGFKSFDLTFEIKNLFDNKEVRDAFDFPLPGRSFFVTASYRF
                     "
     regulatory      complement(7477..7679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7800..8978
                     /locus_tag="AC159U_10570"
     CDS             7800..8978
                     /locus_tag="AC159U_10570"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3337532.1"
                     /translation="MNIYKPSESKEAPWDTYYFLEDHNQTSEYGYAIMEGKILSKSTY
                     IQCTQDELVNAAENNKDIYSAWTPYSNKLIPLETISFLSDVIKRKTFNDLKAKIKEGY
                     QITVFFGILTVIDVFVFKDKGPSLTPLLFLIFGVIPVISGRYELSRLDNITFEDRAYD
                     ILYNRWIGKKVSRPLIAFCIVLVLIAILQFIFGLSNSVELAGLVKEKTWNGEYFRVVT
                     ATLLHGGILHIIFNGLVLFSIGQLINAITHFSLIGLIFTLSAIVGSIFSLFLLPNATS
                     VGASGGILGCIGFLLIITYYKRKIFPKEFFKSVITDVLIISLMGIVGYQIIDNAAHLG
                     GFLTGCLLGFVILRDKEKTSLPFKPNEIIITLGYLSWLFLILASLFTIYKITPLQFNI
                     "
     gene            9003..10544
                     /locus_tag="AC159U_10575"
     CDS             9003..10544
                     /locus_tag="AC159U_10575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3337533.1"
                     /translation="MSSGIESIDLIKDKAQGIVADSKSRLKHCYYIDVRVGAHHIKTA
                     TAEKGVAKSSSDETDLSLGIRVIAGDKMLASGFSGRFLGPGDFSKFDKILKESIEKAH
                     SRALINSKHKHDIKSSLGNLSDSLRSTDLGKIEVYQDEVKVSFEQNPHDTSLNKIVNE
                     AVLISKDIEEQYSKNGYNVVSLSTEIIRELFVSTEGSFIDQTFPLTEGFIYVLVGDEY
                     NYDQLGAQGGWEVLEGKNVFRTDIKQFATYLTAETIETSKADIIEPPDKDVIVVTDPH
                     FNALVVHEIVGHPVEADRALKMETAYAGRSWLLTNLKENQIGKQIASPLVTAYSDSNI
                     SGYGFYGYDSEGVKGKRVTHIENGIFRGFLNSRESAAILKEQPNGAMQTSGSQYIPVV
                     RMTNTVFDGGGSDPEEIIKEVDDGYYLQGHHTPSIGESRENFSISARRVSKIEKGEIV
                     KTYRSGGITSDSKKYLMSIDAVGNDFVLYPMPNCGKGQPMQVKRVGNGGPTMRGIAKL
                     TGGRK"
     gene            10541..12007
                     /locus_tag="AC159U_10580"
     CDS             10541..12007
                     /locus_tag="AC159U_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3337534.1"
                     /translation="MIELDDLRKVVKQGLDYIKSQSDIQDVEVFVSWYDNITVRLNYT
                     SDIPCSGVQEPKNILGYGVGVFASFKTTEGLKTGYGSETADISLDGVKNALSKARVNA
                     VLDPDYKSLPDTSSDTKDIQTADINFDNEVSEEHIISLGWKTLESAIKEFQKMNYSKS
                     LIVGGDINILLEKMAVGNTNGLFASDTSQFISSAVTSMIENENAKGTGWNVGNSINDF
                     DPTIAGSMAAKSAINSIGGKRIESGKYKVIFGPQAVAMLFSEMIISALSLNSIDKGNS
                     PYGKKIGQIVANKNLNIYDDGSMKEGPASKRFTCEGVLTGRTDLIEKGRLVGFLTNNY
                     YAKKLQSNIIEFPARNGFKFGRGGGRDYKRQNSIYATNFVIEGNRETDSAELLSKVKN
                     GIYIGRLWYLYPVYGLAKADFTGTVIGDSYIIENGKIISALKPNSVRITDNFVKLLND
                     VIGISKEKKPTQLWDAEEVIHAPEIAVKGIRLNSIADI"
     gene            12040..12432
                     /locus_tag="AC159U_10585"
     CDS             12040..12432
                     /locus_tag="AC159U_10585"
                     /inference="COORDINATES: protein motif:HMM:NF022526.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HIR3337535.1"
                     /translation="MDLIELLIKNLGVNHTQAMGGAGLIFQVAKDKLNSSDFSEIAKH
                     VTGIEDILASAPKTGGFLGKVGGLASGLGSGGEKLAVISKLGAGFSKLDLGSDMAMKF
                     FPVFESYFREKNDDAVNSIIDKVLGKMG"
     gene            complement(12441..>13236)
                     /locus_tag="AC159U_10590"
     CDS             complement(12441..>13236)
                     /locus_tag="AC159U_10590"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3337536.1"
                     /translation="DTTAEKERRESELQIAHDIQMGFLPQKFPPFPERNEFEIFANLI
                     PAKHVGGDFYDFFFLQDDEFTYKGNNKLFFVIGDVSGKGVPASLFMALTKTLLKAKAS
                     SNIKPSQLLAAVNRDLCVDNESCMFVTVFCGVLDVDSGEFEYSNGGHNPPYIVSKTRG
                     VRMIEGETGIALGIHDEAKFVTYKEKLSVDEGLYLFTDGVTEAMDIDYNLYSEERLEK
                     FLGKLNGCCETELLVESSKAEVVKFTTGAEQSDDITIMAIKYLSQS"
BASE COUNT         4113 a         2469 c         2578 g         4076 t
ORIGIN      
        1 cggagctact gcgatatgtg ttgataacaa agcaggttat tgttttcaat ttgcaaatga
       61 acttttaacc tctgatgtaa gttttgtttt acaggctgat tttggatgga aaggttcacc
      121 agcagcaact aatgtttcgg gcagaaaaga ttttgcaatt tattcagatg gttctataag
      181 gtgtagtaca tcactggttt cttcaagaaa tcccggagct tttgaggcta acaggaattc
      241 acttgcatgt gatgattaaa ttatcacttc ttttttgctt ttaaccaaaa gccataaaaa
      301 gaatattcca cctataaaag cagtaacaat tcctaccggt atttcaaccg gtgatattat
      361 tgttctcgat attgtatctg taattgttag aaatgatgca cccaaaagaa atgagcatgg
      421 gagaagaatc ctgttatcaa ctccaattat cattcttagt atatgaggaa tgaaaagtcc
      481 tacaaaacct attggtccgg tgactgatat cacagccgca gcagcaagag acgtagcaaa
      541 atagattttc aactccattt ttttaatgtt tactccaagt gaagaggcaa cgtcgtttcc
      601 cagggaatat atattcatat cctttgtcat tgatataagc aatagacatg ctggcactgc
      661 aagcaaggct gttttccata ccagtgaaaa tcctataata tccaggcttc ccataatcca
      721 ctttagagtg tgtagtgatt cagtgaattt ggaaaagaac tgaataaata aaatgaaagc
      781 agaaaataaa aagttcatta ccaccccggc taaaagtaat ctgcccgtag ctacaacacc
      841 gtttactttt gaaactgagt agacaaataa aatagtgagt attgaaaata taaaagaaaa
      901 aacagaaatg agtgaaaagc ctaaaatact atatgcaaaa ccaagcttga tagcaattaa
      961 agctccaaga gatgcacctc ctgatactcc aagagtaaat ggtgatgcaa gagggtttct
     1021 aaacattgcc tgaagggcag ctccgcaaac agccagagtg ccgccggcta tgctagcaag
     1081 cagtatacga ggaagcctga taccaaagaa cactttttta tctatttcgg aaagtgagtc
     1141 aaaattgctt aataattcaa gcttaaatgt tcctgatgaa atgctaatta gtacaactac
     1201 tgtccaaaga gccagtaaaa ttaaaaaagt aagtattagt ttattttttt gtgtagctga
     1261 agaattcagt atcataaaat atttattatt ttgagtttta gttcaaggta ttagccgtta
     1321 tgagcagact ggaattgtcc cttagtattt ttacatctgt atcgtaaact tctgtgaggt
     1381 tgtgctcggt aagtacatct gaagaatttc ctgaatataa aacctttccg ttttttaaga
     1441 gtatggaaat accgagggtg ttaataattg agctaatgtc gtgggttgcc actattattg
     1501 ttattccttc atctgtacat agctttttta ttatagaaaa tatcatggat ttatgtttta
     1561 ggtctaaaaa agtgcccggc tcatctaaaa ccatcacctg aggctgctga gcaatggctc
     1621 ttgcaagtga aaccagctgt ctctcgccgc ctgatatatt atttatgttt cggtcctgaa
     1681 gatgagtaat acccacttgt tccatcgcac tctttgctat ctcaaaatca ctgtctcttt
     1741 caaactcata tcttccaata tagggagatc ttcccagaag aactacctct tttaccgtaa
     1801 aaggaaatcc aaaagtggtt tcctgagata cgtaggcgat ttgttttgca agagcagctc
     1861 tgccgatagt tttaatatca gtgtcttcat ataaaacata gccgctatca ggatctagaa
     1921 ttccacagag aacttttaac aatgtagatt tgccggctcc gtttgcaccg aggatatatg
     1981 ttatttctgc tctttttata gatatttcaa tattgctaag aactggattt ttcatatatg
     2041 aaaaatctat attatttgtt tttattatct cattcataat cagtgtgcaa gttgagggtg
     2101 aataatattt ataagctctt ttaaaccagc aataattctt tggcttggat gaagaatggc
     2161 actactggat ataacactca attgatcatt tttaatagct cttgcttcag aaagcgctga
     2221 ccatatttgt ttaacttcct tttctttatc ggttttatca tggtttatct caattattat
     2281 gtctggatct aaagaaaaga tagcctcttt ggagattttt aatgcagttt tcttcttttc
     2341 aattacattt actccaccgc taacagtaat aagctcattt atatagcttg tattaccaac
     2401 cacataaatg tcctcgagtg tgccggggtt tctaccaatc acacataata cagtttttgg
     2461 ctcttgtttg gataatattt cttttattgt tgtaacctcg gattcaatct gctctttgag
     2521 cctgatggat tcgtcttgct tacccagtgc ttttcctatc gtgattatag attcatttat
     2581 atgtgcaatt gatggattac tatctacact cagcgtactt agtccaagtg acctcagctt
     2641 atcaccgaat atattgtcct gatcggttag catgataaca aggtctggtt gcagttgtat
     2701 aatcatttcc aggttgggat taacccaccc gcctacattt tgaatatctt taacctctgg
     2761 cgggaaatct gagtacattg tcacaccaac taccttgttc caggcatcca gtgcatatat
     2821 gatttctgta atacttggag aaagtgatat cacacgatcg ggatattttg actcagaata
     2881 tagaaaggtg gtcggaataa aaaagaatac gaatatgaag caaataatag gtctaaaaat
     2941 atagttcatc ctaacccaaa aagtcctgca gatatatgta gtttctataa ttaataaatg
     3001 aatcagccgg aaaatcaggg ccaaaaaggt caggatgtaa tatatgagcc agtatttcaa
     3061 tgccggttac tattctgggc ccggggcgtg aaaagtatga attagcatct acgatgtatg
     3121 catgtccttt tctggtagat ggaagcttgt gccactgatc aactgaaaga aggctgtcca
     3181 tttcatccag gcttttatcg atgtcgtaac cgcagggcat tactattgta tatctagggg
     3241 tacttttaag cacatcgtcc catgtgactt taagtgacgg cttccctttt cttcctagta
     3301 cgggattacc tcctgcaagc tctaccatct cgggcaccca gtgtccgccc acatagggtg
     3361 gatcaaacca ctcgagtgca aatacttttg ggcgttcgtc ttcaatctcg agtttttgtt
     3421 ttatcgtatc taatctcttg ctaagtttgt catgaagttc tttagcttca ttttctttat
     3481 tagttgcctt acctacagtg ataaaagagt ccagaacttc tccaaccgtt ttaggctcaa
     3541 gacttactat ttcaggcatg ctgccgagtg cctttgctgc attcactact tcatttccag
     3601 acacagcaca tacttcgcaa aggccctgcg taataattat atccggactt gcttctttta
     3661 atttctctaa ctctattatg taggtacttt tgccttcttc tttattcttt gctaccagat
     3721 catctatatc tttactgttt aattcttctg gttttaagaa gctgtttatt acacgtagtt
     3781 tattactaac atcttcggga taatcacact catgtgttat acccgtaagg ttttcaccaa
     3841 gaccaagctc atacagtatt tcagttgcgc ttggaagaaa agagcatatt cgcattaatt
     3901 agacctccac ataaaaatat tggagagtct aattaatcta tctcttagat gcaattgttt
     3961 catgattaag gtataaagag tgatgtatta atagaaccaa gctgttcgct aattcctgtg
     4021 ataaaaaaca gatcaggatt ttgtccatta tcagttattg ccattacctg aggcccatca
     4081 aaaaaatcag atgaagggtt gtctcctcta agccctacag gaaattctcc aagaaaagga
     4141 aacgggctta tatcatcaat ctcagggtcg aatacaaata ctgtatcgga attaaacatt
     4201 gccacaaatc cgtaaccgct tgagctgaat tcaatgtcaa aggttgcatc caatccttca
     4261 gtatcggtta ttattatagg gtctccagaa cctcttataa ggtcatttgt atttagattg
     4321 atcttaaaca gcacaccgga aatctggctt cctatatatg caaaatcacc gtcaggtgat
     4381 gctgaaattg attctggaga acatacagtg ggatttatct cactaagtgg aaattctata
     4441 gagtcttcaa ctgtgtcaga tgcagtgttt ataacatcga tactgatagg agattcagta
     4501 tcacaagaaa aaagacctgt atcaaaatca aacagtacat ttccactgtt gactacataa
     4561 actttatcat taaataacat aattccttta ccggttccgc ttgtgccttc ccctgtagca
     4621 tctataactg tatctatctc attagtctca gaatcgatca ctgtgataaa acctttaccc
     4681 atgggcgcaa atgttgagtt atctatattt tgatttgcaa cataagcttt attattagct
     4741 attacaattc cagcagggtt tgcaaacgtg ccttcgtcta caatgataac ttcgtcacag
     4801 ggagtgaaat taccttcatc ctgactaatg atagtttcac atgtcatatc ctgtatatta
     4861 acaattgcta ctgtctgtga aatcaaattt gtaacatagg catgttcgtc attaaaaaac
     4921 gctatcccta cagggtccga tcccagaggt aatactatag tgccagtttg cacaggtgga
     4981 ttttgtgtaa tatcgaaaac ctgaatgtta tcggaaaagc cattcaggac atatgccctg
     5041 ccgttaaata tttttatgtt tgtaggtaca ctgccaagct gagcaaggtt tctatttaca
     5101 gctccattca tgagtttaat tcttgtatcg agcggaatat caataaaaac ctgactgttt
     5161 acaatgatac tcttaccacc tgaaacaatc tcgaactcat caaacacaga acccgtgagt
     5221 gctgctataa aaccgcccct gctatttgcc cttactactc tttgctctgt agtatccagg
     5281 ttagtgatgg atacaaacgc gttagaatta actgcaccat ccaggccacc tacaactacc
     5341 gttgtgtttg ggtcgacatt ttgattaagt tcgctgttta gaatcatcac agggccgtta
     5401 ttaagtgcaa agaatttgtc tggatcaaac tcatcatcgc tcccgccgca tgctataaaa
     5461 tagacagatg ctattagtgc taatgacaaa catagtatat ttttcataaa tatctccttt
     5521 aaaacctgta tgaagccgta acaaaaaatg atcttcccgg aagcggaaaa tcaaaggcgt
     5581 ctcgtacttc tttattatca aatagatttt ttatttcgaa tgttaaatca aaactcttga
     5641 atcctgattt tgcgcctgtg tcgtgtgtgt atctgttttc tgttcttctt gcgtttggaa
     5701 aagcactgag aggaatctcg tctacaaaat gtgattccca aaatagtgag gcgtatttaa
     5761 tatccagatc acctcggaaa tcaaatttgt gttttggacg accgggaagc tgtgtgccgg
     5821 tatcgtcaag tgttccatct aacagtgtgt atgctgaata aagctcgaga aagtctgttg
     5881 gcctcactcg caggcttgcc tcaagtccct gttggtctac atcgccaacg tttctaggtt
     5941 ctattctctg ggcgcttaca aaaacaaaca atatcaggtc atcaatgtta ttcttgaagt
     6001 aattgacctc gaatgcgaac cgtgggcggt ttacaacgag acctatatca aaatctatgg
     6061 aggtttcgga ctcaagatta ggattgccgc caataaaccc ctgctcaggg aagaaaagct
     6121 cgccgaagtt tggggcgcgg aacgaccggc ccacatttgc cttaagagct atccagttaa
     6181 atggtgaaac cataagacca agcttaggag agacctgagc ttcagtgtca ttttcatcag
     6241 caaatgtctt gaatacatcc agtcttacaa gtggatggag agtaatgaaa ttatagaaaa
     6301 aactaaattc atctcccagg tatgaactga atgtaagtct ttcaggattt ttgaaatcgt
     6361 catcctgtag aacatctgcc ttaagttccg ttgcaaaagt aatcgtgtgc tcttttggag
     6421 caaaccatgt gaattttgtg ttgaaaccga atgagtagtt ctgactgagt gtatcaatcg
     6481 gaagtccaag aacgggctca ggatttttaa actccagctc atcgaatctg ttaaaaatta
     6541 tcgtctcaaa attaagtgag ggtgtaaaca acccatcttt tgctatcttg agtgatgtaa
     6601 gatttcttat gtcctgctga tttgagtttg gctcctgaaa ctctcccaac cccgggatgc
     6661 ccttgtcatc ataataaaag tcattgaaca ggctcaaatt ccagtcacct agttcaaagt
     6721 ccagctttga atagaagctc tcggataaaa actcattatt tattctcttc acatcgaagc
     6781 cgtttattga ttcaaactca aagtctccct cgctttgtgc gtgagtatag gagaagaaat
     6841 atccaagttc tccaaagctt ccggcaccgg acaaattggc cttaaaagta tcaaacgatc
     6901 cgtatgtggc agatgtggaa aaatatttat ctcctgtttt tcttggtatg aggttaacta
     6961 ctccgccaat tgcatcagaa cctgctagtg cagagccgcc gccccttact acctcaattg
     7021 attcgatata atcgatcggg atagtagaca ggtctatccc gccgccaatg gggcttgata
     7081 gcctcacccc atccaatagc acaacgacct ggtcgtttga cgatcccctg atagatagtg
     7141 tctttaactg accaagccct ccaaaatctc ttacaacaac cccgggactt aaatttagca
     7201 gttctgttgc ggtgttaaat tctccaagat atgcatcaag gtctattacc gtggaaaagg
     7261 cggaggggta atccagcggc ggcctcagat aggtgtcctc tactatcaca ggttctaact
     7321 cctggccggc atcctgtgca cgtactaatc ctgtttgaat taatagtatt agtaatgtta
     7381 tgaaaaactt catttgccat cccatctctt ggggaaaaga cggaacaaca tgtatctatg
     7441 tataatcatt tactgttctc ccctcgaaga ataagtaata ttttaggttt ctatggcagg
     7501 tcttctggct ctcagtgttg ttctttgatt gtttttggat aattctttgc gccttcccag
     7561 aggttttgcc tccagtggca actgcaaaga cttttcactg attacagcag cggggctgcg
     7621 ccggcctcta accggacttc ccttttaata catgcgtaac ccagaaactt atttaattgt
     7681 taaagatcaa taatgcttac ttttagacaa tatactttca gtagaaaaaa atcaagagaa
     7741 atatttggta gtatcaaaat ttataatatt cttttgtata atctgacacc ataaataaaa
     7801 tgaatatcta taaaccatca gaatcgaaag aagctccttg ggatacatat tattttctag
     7861 aagatcataa tcaaacgtcg gaatatggtt atgctatcat ggaaggaaaa attttatcta
     7921 aatctacata tattcaatgt acacaggatg aattagtcaa tgcagctgaa aataataaag
     7981 acatttattc agcttggaca ccatattcaa ataagttgat tccacttgaa acaatttcat
     8041 ttctttcaga tgtaataaag agaaaaacat tcaatgattt aaaagcgaaa ataaaggaag
     8101 gatatcagat aactgtattt tttggcatct taactgttat agacgtattc gtttttaaag
     8161 ataagggacc aagtcttact ccactacttt ttttaatatt tggagttata cctgtaatat
     8221 ctggcaggta cgagctttca cgactagata atataacttt cgaagataga gcttatgata
     8281 ttttatacaa tagatggatt gggaaaaagg tttcaagacc ccttattgca ttttgcattg
     8341 tattagtgct tatagcaatt ttacaattca tctttggtct ttctaattcg gttgaattgg
     8401 cgggtctggt aaaggaaaaa acttggaacg gggaatactt tagggttgta acggcaacac
     8461 tacttcatgg tgggatatta cacataattt ttaatggatt ggttttgttt agtataggac
     8521 aattgattaa tgcaatcaca catttttcgc ttattggact tatattcaca ttatctgcaa
     8581 tagtaggaag tatttttagt ctttttttat tacctaatgc tacatcagtc ggggcatcag
     8641 ggggaatttt gggatgtatc ggatttcttt tgataattac ttattataaa agaaagatat
     8701 ttcctaaaga gttctttaaa tctgttatta ctgatgtatt aattatatcg cttatgggta
     8761 tagtgggtta ccaaataata gacaacgctg ctcatctagg tggatttcta acgggatgtt
     8821 tattaggttt tgttatattg agggataaag agaaaacaag tcttccattt aaaccaaatg
     8881 aaataatcat aacactgggt tacttatcct ggttattttt aattcttgca tctcttttta
     8941 ctatttataa aataacaccc ttacaattta atatttaatt ataaaaggtt aaaactatat
     9001 atatgagttc tggaatcgaa agtattgatc taataaagga taaagcgcaa gggattgttg
     9061 cggatagtaa gtctcgtcta aagcactgtt actatattga tgttcgggtt ggggcgcatc
     9121 atataaagac tgcgactgcc gaaaaagggg ttgcaaagag tagcagtgat gagactgatc
     9181 tttctcttgg gataagagtt atagccggag ataagatgct ggcatcgggg ttttccggaa
     9241 ggtttttagg cccgggtgat tttagtaaat ttgataagat attaaaagag tctattgaaa
     9301 aggcacatag cagggcgctt attaattcca aacataaaca tgatataaag tcgtcccttg
     9361 gtaatttgag tgattcactt agaagcaccg atcttggaaa gattgaagta tatcaggatg
     9421 aagtaaaagt ttcttttgag caaaatccgc atgatacatc acttaataag atagttaatg
     9481 aagcggtttt aatttccaag gatattgaag agcagtattc aaaaaacggc tacaatgtag
     9541 tttctctttc aacagaaata atacgggaac ttttcgtctc aaccgaaggt tcatttatcg
     9601 atcagacttt tccgttaaca gagggattta tttatgtact tgtaggagac gaatacaact
     9661 atgatcagct aggagcacaa ggcggctggg aagtgctgga aggaaaaaat gtttttagaa
     9721 ctgatataaa gcagtttgcg acatatctta cggcagagac tatagaaaca tcaaaggcag
     9781 acatcattga accaccggat aaagatgtta tagttgtcac cgaccctcac tttaatgctc
     9841 ttgtcgtaca tgaaatagtt ggccacccgg ttgaggccga cagggcgctt aagatggaaa
     9901 cggcttatgc gggtaggagc tggcttctta caaacctcaa ggaaaaccag ataggaaaac
     9961 agattgcatc tcctcttgtg acagcttatt cggattccaa tataagtgga tacggatttt
    10021 atggttacga ttccgaagga gtgaaaggca aacgtgtaac acacatagaa aacggaatat
    10081 tcaggggatt tttaaacagc agagagagtg cagctatttt aaaagaacaa ccaaacggag
    10141 cgatgcagac ctccggctct cagtacattc ctgtagtaag aatgacaaac acggtgtttg
    10201 acggtggcgg ttccgatccg gaagaaataa ttaaagaagt tgacgatggc tactacctcc
    10261 aaggtcatca cactccgtct attggagagt cgagagaaaa cttcagcatt tctgcaagaa
    10321 gggtgtcaaa gattgaaaaa ggtgaaatag taaagacata cagaagcggc ggcataactt
    10381 ctgattcgaa gaagtatctt atgagcatag acgcagttgg aaatgatttc gttttatacc
    10441 ccatgccaaa ctgcggtaag ggacaaccga tgcaggtcaa gcgcgtggga aatggcgggc
    10501 caacgatgag aggaatagca aaactcaccg ggggaagaaa atgatagaat tagatgacct
    10561 ccggaaagtt gttaaacaag ggttggacta tattaaatct caaagtgata tacaggatgt
    10621 cgaggtattc gtatcatggt acgacaatat taccgtaagg ctaaactata catcagacat
    10681 cccttgcagc ggggtgcaag aaccaaagaa catcctgggc tacggcgtgg gtgtttttgc
    10741 aagtttcaaa actaccgagg gactcaagac aggttatggc agtgagactg ccgatatatc
    10801 tctggatgga gtaaagaatg cactttcaaa agcaagagta aatgccgtac ttgatccgga
    10861 ctataaatca cttccggata catcttcaga tactaaagat atacagacag cagacattaa
    10921 ctttgataat gaagtttcag aagaacatat tatttcactt ggttggaaaa cattagaatc
    10981 tgcaattaaa gaatttcaga aaatgaatta ttcaaagagt ctcatagttg gtggagatat
    11041 taatatactc ttagagaaaa tggcagtggg aaacaccaat ggtctctttg cttctgacac
    11101 atcacaattt atttcttctg ctgttacttc aatgatagaa aatgaaaatg caaagggaac
    11161 cggctggaat gttggaaatt caataaatga ttttgatccg actattgcgg gaagtatggc
    11221 tgcaaagagt gctataaact ccattggcgg aaagcgaata gagtcaggca aatataaagt
    11281 aatatttggc ccgcaggcag ttgcgatgct attttcagag atgatcatct ctgctttatc
    11341 actcaactcc attgataaag gcaattctcc gtacggaaaa aaaatcggtc agatcgtagc
    11401 taataaaaac cttaatattt atgatgatgg atctatgaaa gaagggcctg cttcaaaaag
    11461 atttacttgt gaaggagtgt taacaggaag gacagattta atagagaaag gacggcttgt
    11521 aggatttctt accaataatt attacgctaa aaaactgcaa tcaaatatta tagaatttcc
    11581 ggcaaggaac gggtttaagt ttggcagagg tggtggaagg gactataaga gacaaaattc
    11641 catttatgca accaactttg taattgaagg aaatagagag actgattcag ccgaattatt
    11701 atctaaggta aaaaatggaa tttatattgg caggctctgg tatctttatc ctgtgtacgg
    11761 acttgctaag gcagacttca cggggacggt cattggtgat tcatatataa tagaaaacgg
    11821 aaagattatt tctgcactaa aacccaactc agtacgaatc actgataatt ttgttaaact
    11881 attaaatgat gtaattggaa tatcaaaaga aaaaaagccc acacagctct gggatgctga
    11941 agaagttata cacgcacccg agatcgcagt gaaaggtata agacttaaca gtattgcaga
    12001 catataatct gtatagatat agtattacgg gagagaaaat tggatctgat agaacttcta
    12061 attaaaaacc tgggagttaa tcacacacag gctatgggag gagcggggtt aatttttcag
    12121 gtagctaagg ataagcttaa ctcctcggat ttttctgaaa tcgctaaaca cgttacaggt
    12181 atagaagata tactcgcttc ggctccaaag accggagggt ttctaggaaa agtgggaggg
    12241 cttgcctcgg gacttggaag cggaggagaa aaactggctg taatttccaa acttggcgca
    12301 gggttttcca agcttgatct gggaagcgat atggctatga aattctttcc tgtattcgag
    12361 agttatttca gagaaaaaaa tgatgatgca gtaaatagta taatcgataa agtgcttgga
    12421 aaaatgggtt aattcaagcc ttatgactga ctcagatatt taattgccat aattgtaata
    12481 tcatctgact gctcggcgcc tgtggtaaac tttactactt cagcctttga actttctaca
    12541 agtagctcag tctcacagca tccgtttagt tttcccagaa acttctcaag acgctcctcg
    12601 gaatacagat tatagtctat atccatagcc tctgtgacgc catctgtaaa cagatacagt
    12661 ccttcatcaa cactaagctt ttctttatag gttacaaatt tagcttcatc atgaatacca
    12721 agagctatac ctgtttcacc ttcaatcatt cttactcccc ttgtttttga gactatatat
    12781 ggcggattgt ggcccccgtt actatattca aactctcccg aatcaacatc cagaacacca
    12841 caaaaaacgg tgacaaacat acagctttca ttatctacac aaagatccct gtttacggct
    12901 gcgagtagtt gactaggttt aatatttgaa gaagcctttg cttttagcag agttttagta
    12961 agagccatga aaagtgatgc tggtacacct ttgcccgata catctccaat tacaaaaaaa
    13021 agtttgttat tacctttata agtaaattca tcgtcctgca gaaagaaaaa atcgtagaaa
    13081 tctcctccta catgtttagc aggaattaga tttgcaaata tttcaaattc gtttctctca
    13141 ggaaacggtg ggaatttctg cggcaggaaa cccatctgta tatcgtgtgc aatttgcagt
    13201 tcactctctc ttcgttcttt ttcagcggtt gtgtct
//
LOCUS       DBNGSV010000285         2632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1021054, whole genome shotgun sequence.
ACCESSION   DBNGSV010000285 DBNGSV010000000
VERSION     DBNGSV010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1021054"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..861)
                     /locus_tag="AC159U_10595"
     CDS             complement(256..861)
                     /locus_tag="AC159U_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337537.1"
                     /translation="MYEITRLINHVICGSDSKKKEIVEKLGYKEDMYRGYSRLRGLMQ
                     TGKCDSEIRENLPQALGIDKKFVQEAFDKTDKKLNKQRDIHSSNRHKLERRRFRPYLW
                     IEHEYDHIISKSYMNSSRYNDIKKIELPEFISSLKWEDQLKVIRRYINMNQKSQKDKR
                     LGEVKNYLYFKDSDQSYLFDAYGGHVTTNNAVQRDLLFAVS"
     gene            989..2020
                     /locus_tag="AC159U_10600"
     CDS             989..2020
                     /locus_tag="AC159U_10600"
                     /inference="COORDINATES: protein motif:HMM:NF052219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3337538.1"
                     /translation="MIDIPEPIGKYDRFYYLIECLSNHKTGVTVEEIAEAVYKSEKTV
                     RRDLKSIEGNIFGINLIKDRGPDNRYRYRIEKQAATFRPLLLNTYEVLALYFIRGFAH
                     FKDIPFMQSNLKEVFNKIDLTATEAKKNAGNDFFNRVSNLFILPRELGGKIYGEKSRL
                     IFLEKIIEAALDFRICEITYGKGKKEKKYRIAPLHFFNYRDAIYVLARNLSKPEYEKE
                     NIIVSMALHRIKELEIIEDNFDYPGDFDVEEYFDGSLFKFEDKKHSIKLLFSPEIKDY
                     ILEREWYPNQMERLYKDGSVELKFESDINMILTGWIKGFGSNVKVLEPKELADEIIID
                     LKDNIDQYN"
     gene            2190..>2632
                     /locus_tag="AC159U_10605"
     CDS             2190..>2632
                     /locus_tag="AC159U_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017443971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3337539.1"
                     /translation="MKNSTYFKKNFAVLGLVVFALVLTLFVFSKSSVAEKSDAAYKFN
                     EKGELIKPEGYREWIFVGTPVTPNDMNNGKAPFPEFHHVYIDPVSYKHYKETGKFREG
                     TILMKELISVGSKSAVSGNGYFEGEFLGLEATIKDSKRFPKEPGN"
BASE COUNT          890 a          412 c          476 g          854 t
ORIGIN      
        1 ctgctccaaa agctttaata caagacaaaa aaaccacgaa ctatgaacaa actttcactc
       61 caactcactg acaaaatgtt aacaaattct aactttaaaa ctggacctaa attctggggc
      121 ttgacaaccc tttccgggtt caggatgggg tatgttaagt ggcaaaactt aaagtgtttt
      181 tttggctcct taccaaaagg ggtgtattaa taataaggag ccggagtggt tattgctgtt
      241 ttaatggtat aggttctaac ttactgcaaa gagtaaatcg cgctgtaccg cattatttgt
      301 tgttacatgg cctccatagg catcgaagag gtaactttga tctgaatctt taaaataaag
      361 atagtttttt acctcaccta gtctcttatc tttttgactt ttttgattca tgtttatgta
      421 tcttcttatc acttttaact gatcttccca cttcaaagaa ctaataaatt caggaagctc
      481 aattttcttt atatcgttgt aacgggatga attcatataa gattttgaaa ttatatggtc
      541 atactcatgt tctatccaaa ggtaaggcct gaatcttctt ctctcaagct tatgtctgtt
      601 tgatgaatgg atatctcttt gtttattcag ttttttgtct gttttatcga atgcttcctg
      661 tacgaatttt ttatcaattc ccaatgcctg tggaagattc tctcttattt cagaatcaca
      721 ttttcctgtc tgcataaggc ctctaagtct gctgtagcct ctatacatgt cctctttgta
      781 gcctaatttc tccacaattt cttttttctt actatcactt ccacaaatca catgatttat
      841 aagcctggtt atttcataca ttttgcacct ccaatatttg tagaatatta attatttata
      901 aattaattat taaatagcca atggacattt tgtgtccaac agtaaaaata tttttaaaat
      961 agtaaaaaaa gtttggagga aagggaaaat gatagatatt ccagagccga ttggaaagta
     1021 cgacagattt tactatttaa tagaatgtct ctctaatcac aagacaggtg ttacggtgga
     1081 agaaattgca gaagctgtat acaaaagtga gaagaccgta agaagagacc ttaaatcaat
     1141 agaaggaaat atctttggaa taaacctcat aaaagacaga ggaccggaca acagatacag
     1201 atatagaata gaaaaacagg ctgctacttt ccgccctcta cttttaaata cctatgaagt
     1261 tttagctcta tatttcatta ggggatttgc ccattttaaa gacattccat ttatgcagag
     1321 caatttaaaa gaggttttta ataaaattga tcttactgct acagaagcta aaaagaatgc
     1381 aggcaatgat ttttttaacc gcgtatcgaa cctgttcatt ctgccaagag agctcggtgg
     1441 caagatttat ggtgagaaga gcagacttat atttctggaa aaaattattg aagctgcgct
     1501 tgatttcaga atatgcgaga ttacttacgg aaaagggaaa aaagaaaaaa aatacaggat
     1561 cgctccgcta cattttttta attacagaga tgcaatatat gtgcttgcaa gaaacctcag
     1621 taaacccgaa tatgaaaagg aaaatataat tgtaagtatg gcactacacc ggattaaaga
     1681 gcttgagata atcgaggata attttgatta ccccggagat tttgatgttg aagaatattt
     1741 tgatggcagt ctttttaaat ttgaggacaa aaagcacagt attaaactgc ttttcagccc
     1801 ggagataaag gactatatct tagagagaga atggtacccc aatcagatgg aaaggcttta
     1861 taaagacggt agtgtggagc ttaagtttga aagtgatata aatatgatcc ttacaggttg
     1921 gatcaaaggt tttggatcaa atgtgaaggt tttagagcca aaagagctag ctgatgaaat
     1981 cattattgat ctaaaggata atattgatca gtataactaa agactatcgc ccttctgtaa
     2041 tttattcata aatattacat attctgaatc catttaatat tttgatttgt ctatttaaat
     2101 aagagatgaa tattcagaat atctcagatt caattaataa atatctacaa aacaagatca
     2161 taacttgtta aatgataagg aggcgtacca tgaagaacag tacatatttt aaaaagaatt
     2221 ttgctgtttt gggtttagtt gtttttgcat tagttttaac gttgtttgtt ttttcaaaat
     2281 catctgtagc agagaaatct gatgctgcat ataaatttaa cgaaaaaggt gagctcataa
     2341 agccagaagg ttacagggaa tggatttttg ttggaactcc tgtaactccc aatgacatga
     2401 acaatggaaa agcccctttt ccggagttcc atcacgtata tatagatcct gtaagttata
     2461 aacattataa agaaacgggt aaattcagag aaggaactat acttatgaaa gaacttataa
     2521 gcgtcggttc caagagtgct gtgagcggta atggttattt tgaaggtgaa tttttaggac
     2581 tggaagcaac cattaaagat tcaaaaagat tccctaaaga accaggcaat tg
//
LOCUS       DBNGSV010000286         1900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1643407, whole genome shotgun sequence.
ACCESSION   DBNGSV010000286 DBNGSV010000000
VERSION     DBNGSV010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1643407"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="AC159U_10610"
     CDS             <1..1229
                     /locus_tag="AC159U_10610"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR3337540.1"
                     /translation="HATVHVGIERVSRLFSSLLETSNEYLSFTEYSQRYQKPQKGDFY
                     VPESLKGNKKLLSEYIELNNKVYDIYSQLNTKLFEYLKKSVPVPDGNDEKSHFRALEK
                     LAFEDARYALNLATFTNLGMTGNARAIEDTLSCLLSSKYPEAVSRAEEIKEEVRFSVP
                     TLVKYANENKYIKETREDFENLIKLDTTGGSKSRRKSKVKLLNWTGIDSADAQVAALD
                     KIVNAILYQHSELGTESLLGELRKYDLEKKLLLLRMSVEKITKFDNPLNVFRLIDYEA
                     EFILSEACWHQLLRHRKVKWIIKEPGINHGITVPPNIEAAGITDLLIEAVEISESLYT
                     KLQAQSLITEAAYVVTNAHNRSVLGSFDLWELYHLINLRMSEAAQWDIKDVIGNLAEQ
                     IRQIHPDLVEPALSRI"
     gene            1257..1760
                     /locus_tag="AC159U_10615"
     CDS             1257..1760
                     /locus_tag="AC159U_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3337541.1"
                     /translation="MANENHLEILGQGAEEWNKWRQENPDITPDFGWANMPAMDLRGY
                     DFENANLKLAFCKSCNFTGANLKNSNLYGANLENTNCSKADFSGANLEGALIKDAVLT
                     GSNLSDCNFRLVNLEGSNFEGADFSGCKKLKADQLSKALSLYEANLNERLLEKLSQTS
                     PNLLIKS"
BASE COUNT          635 a          337 c          404 g          524 t
ORIGIN      
        1 agcatgcgac agtgcatgtg ggaatcgaga gggtatccag acttttttcc tcccttctgg
       61 aaacttcaaa cgaatatcta tcttttactg agtactcgca gaggtatcaa aaacctcaaa
      121 agggtgattt ttatgttcca gagtctctta aaggtaacaa aaaattgctc tcggagtata
      181 ttgagcttaa caataaggtt tatgatattt actctcagct aaacactaaa ctctttgaat
      241 atctaaaaaa gagtgtcccg gtgcctgatg gaaatgatga aaaatctcat ttcagggcgc
      301 ttgaaaaact ggcttttgaa gatgcgcggt atgctcttaa ccttgctacc tttacaaatc
      361 tgggaatgac cggaaatgcc agggctattg aagatacgct ttcctgtctt ctttcgagca
      421 aatatccaga agctgtaagc agggctgagg agataaaaga agaggtaaga ttttcagtgc
      481 caacacttgt aaagtatgca aatgagaata aatacataaa ggaaaccaga gaggattttg
      541 aaaacctcat taaactggat acaacgggcg gttctaaatc aaggcgcaag tcaaaagtta
      601 aacttcttaa ctggaccggt atagacagtg ctgatgccca ggtcgctgca ctcgataaga
      661 tagtaaatgc tatactctat caacattcag agcttggtac agagagcttg cttggtgaac
      721 ttagaaagta tgaccttgag aaaaagcttc ttctgctcag gatgtctgta gagaaaatca
      781 ctaaatttga taaccctcta aatgtgttca ggcttataga ttatgaggcc gaatttatac
      841 taagcgaagc ctgttggcat cagctccttc gtcacagaaa agttaagtgg atcataaaag
      901 agccgggtat aaaccatgga attacagttc ctccaaacat agaagctgcc ggaattacag
      961 atttgcttat agaagctgta gagattagtg agagtttata cactaaacta caagcccaga
     1021 gtttgataac agaggctgcc tatgttgtta caaatgccca taaccgctct gtgcttggaa
     1081 gctttgatct ttgggagctt tatcatctta taaacctaag aatgtctgag gctgctcagt
     1141 gggacataaa agatgtgata ggaaatttgg cagagcaaat cagacagata catccagatt
     1201 tggtagagcc tgccttatca agaatatagt aaaactttta tttaaattat ccgaatatgg
     1261 ccaatgaaaa ccatctcgaa atactaggac agggagcaga agagtggaat aagtggcggc
     1321 aggaaaatcc tgacataact cctgattttg gctgggctaa tatgccggca atggacctta
     1381 gaggatatga ctttgaaaat gcgaatttaa aactggcgtt ttgtaaaagt tgtaacttta
     1441 caggtgctaa tcttaaaaat tctaacctct acggcgctaa tcttgaaaat accaattgca
     1501 gcaaagctga ttttagcgga gcaaaccttg aaggggcttt aataaaggat gctgtcctga
     1561 caggctctaa cctttctgac tgcaacttca ggctggtaaa tctcgaaggt tctaactttg
     1621 aaggtgcaga tttttctggc tgcaagaagc taaaagcaga tcagctttct aaggctttat
     1681 ccctttatga agctaatttg aatgaaaggt tattagaaaa actttctcaa accagcccga
     1741 atttattaat aaagtcttag gatttataat gtataaggag ctcgcatagg acagcaaagt
     1801 atactaagct gaaactatgc aaaagaagtt acttaagaat acaaaactat caaatagtaa
     1861 gataagcaag ctaatacaaa tgtttgtatt ggaagtgccg
//
LOCUS       DBNGSV010000287         2184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1782292, whole genome shotgun sequence.
ACCESSION   DBNGSV010000287 DBNGSV010000000
VERSION     DBNGSV010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1782292"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="AC159U_10620"
     CDS             complement(<1..353)
                     /locus_tag="AC159U_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337542.1"
                     /translation="MLNLEELLDKEVIEFIGEVEQLDEYKALHGGTITDETYIRFLKT
                     FYIMESISADAVHRASQNTKQTNPYLSQRFDFCAEGERGHAEIALKDLLDMGISGVDT
                     NGNGIVNKYNDFLQTG"
     gene            complement(523..1377)
                     /locus_tag="AC159U_10625"
     CDS             complement(523..1377)
                     /locus_tag="AC159U_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3337543.1"
                     /translation="MSAISEFTQSYFLWKEAVAAGAVIGALCGFLGVSVVLRRIIFVS
                     AALTQVSGLGVAFAFYIQAFAVGLAVPFANPTISSLIFTLIAAYLFAIKKDSLKITKE
                     GVIGFGFLLASASVLILGDKLTHGAHDISDIIFGTAVVVSEKELYYILIVALVVGALF
                     RKYHKDFIFVSFDPDSATLFKFPVKLANALLLVSIGAVISISTKAIGALPVFGLLTLP
                     ALCSLQLTEKLSRIFILSVIIGVVSSVLGYFFSFIFSTPTGASITLTASFILILCSAA
                     GRLRNYQY"
     gene            complement(1374..2096)
                     /locus_tag="AC159U_10630"
     CDS             complement(1374..2096)
                     /locus_tag="AC159U_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR3337544.1"
                     /translation="MITLDKVEIGYENSPLIAPINLSIGENEFWAVIGSNGGGKTTLL
                     KTILGLIPKLSGQILYSPNSSFGYVPQHKNFDKLFPVSVLELVIMGRYSIVGFGKSIS
                     ENDIQIALECLEKVGIAHLAHRTFRSLSGGEIQRALIARALAGQPSVLVLDEPTASVD
                     FKGQTEILDLVSAIRSENKLTVIMVSHYIGSVTEYADRLILIDKQSGVFEQGSVKEII
                     DSKHMQKIFGMSITSGEMGINK"
BASE COUNT          729 a          458 c          355 g          642 t
ORIGIN      
        1 ccagtttgca aaaaatcatt atatttattt acaattccat ttccatttgt atcaacacca
       61 ctaataccca tgtccaataa atcttttaga gcaatctctg catggcccct ttcaccctct
      121 gcacaaaaat cgaaccgctg gcttaagtat gggtttgttt gtttagtgtt ttgtgatgcc
      181 ctgtgaacag catcggcact tattgattcc atgatgtaga atgtctttaa gaatcttata
      241 taagtctcat cagttatagt accaccatgt agagctttat attcgtccag ttgttctact
      301 tcaccaatga actcaataac ttctttatca agtaattctt ctaaatttag catccgatta
      361 ttaatattgt tgagactgca gtatataaat agagataaga aatattacat aagcataaaa
      421 atttaacaat aagacttagg attaattttt attatattca caatatatta aatgattatt
      481 acatatagat tacatctgtg taataagaaa gcactgcatt tatcaatact gataatttct
      541 caatctgcca gcagcggagc aaagaattaa tataaaagag gcagtcaggg tgatcgatgc
      601 ccctgtggga gtcgaaaaaa taaatgaaaa gaaatatcca agaacagaag aaacgactcc
      661 tataatgacc gagagaatga aaatcctgct tagcttctcc gtaagctgta gtgagcaaag
      721 agccggaaga gtaagtagac caaaaacagg aagcgcacct atggctttag tggatataga
      781 aattactgca cctatgctta ccaaaagcag tgcatttgca agttttacag gaaacttaaa
      841 caatgtggca gaatcgggat caaaagagac aaatataaag tccttatgat attttctgaa
      901 cagcgcacct acaactaagg ctacaatgag aatataatag agttcttttt cagaaacaac
      961 gactgctgtt ccaaatataa tgtccgaaat gtcgtgagct ccatgagtta atttatctcc
     1021 caatatcaat accgatgccg aagccagcaa aaatccaaaa ccgattactc cttcctttgt
     1081 aatctttaaa gaatcttttt tgatcgcaaa tagatatgca gcgataagcg taaatattag
     1141 agatgaaata gtggggttag caaatgggac agccaaacca acagcaaatg cctgaatata
     1201 aaaagcaaaa gctactccaa gaccagagac ttgtgttaga gcagcactta caaatataat
     1261 tctgcgaaga acaacagaca cacccaaaaa cccgcatagc gcaccaataa ccgcccctgc
     1321 agcaaccgct tctttccaaa gaaaatagct ttgagtaaat tcagatatag cgctcattta
     1381 ttaattccca tttctccact ggttatactc ataccaaata ttttctgcat gtgttttgaa
     1441 tctataatct ccttaaccga tccctgctcg aaaactccac tttgtttatc tataagaatt
     1501 aatctgtccg catactccgt aacactacct atatagtggc ttaccattat cactgtgagc
     1561 ttattctcag accttattgc acttacaaga tccaatatct ctgtttgtcc tttaaaatca
     1621 acagatgccg taggctcatc caaaacgagc accgaaggct gcccggctaa cgctctggca
     1681 attagtgccc tttggatttc accacctgag agtgacctga aagtcctgtg tgcaaggtga
     1741 gcgataccga ctttttccag acattccaat gctatttgta tatcattttc agatatgctc
     1801 ttaccaaacc cgacaatgct gtaccttccc ataatcacca gctctaaaac tgaaaccgga
     1861 aataatttgt caaaattttt gtgctgcggc acataaccaa aactgctgtt tggagaataa
     1921 agaatctgcc ctgagagttt tggaataagc cctagtatgg tttttaaaag tgttgtcttt
     1981 cccccgccgt ttgagccgat aacagcccaa aactcatttt ctccaatact gagattgata
     2041 ggggctatta gagggctgtt ttcataacca atctcaactt tatccaatgt aatcatgatt
     2101 attttgcaag agcctttaaa atttcatcta taatcttttc gaataaatca atatatgtag
     2161 gcacaccatt atcacgatct acca
//
LOCUS       DBNGSV010000288         1237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1643534, whole genome shotgun sequence.
ACCESSION   DBNGSV010000288 DBNGSV010000000
VERSION     DBNGSV010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1643534"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..>1237
                     /locus_tag="AC159U_10635"
     CDS             272..>1237
                     /locus_tag="AC159U_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3337545.1"
                     /translation="MVEDNLLHRLFVEAEKYDASDIHLTRDAAPYFRVAGRLMSLDSE
                     PLSKQAIAQAVEPTLNDRIKNQYEEKGYVDYAYEPDIGAKVRYRIELYKSRSCMTVAL
                     RRVKSEIPTFEELNLPRVYEKVMHLRPKGIIVIGGETSSGKSTTLAAMMDYINQNEKK
                     HIVTIEDPIEYLIVNKMSWIDQREFGGDFHSYADALRAVVRQDPDVIMIGEMRDRDTV
                     EAAIAAAETGHLVFSTLHTTTATQTFYRILNFFPPDQRYAVRQNLSATLIAIMNQMLL
                     PCKKQGTRIIPAAEVLINTPAVKMYIEKSEENRLPEVMESGTKDGM"
BASE COUNT          440 a          205 c          268 g          324 t
ORIGIN      
        1 acctaaaccg gttggataga atttttagat gatatggaac ctgacactga gaagtataaa
       61 acctgtcttc ccaaaacctt tgggacaata atgaaatttt caagaatata tatattttca
      121 caaaaaaatg acgtatttta ggatttgata tgagcaaaat tgggatttgt agtaataaaa
      181 gggtttcgta atgtcaccta atgaactaat tcaaaaacgg gaaaaggttt agagtactta
      241 ttcgtttatc tctattgtgg gagtgaataa aatggtagaa gacaatttat tgcacagact
      301 atttgtagaa gctgaaaagt acgatgcttc tgatatacat ttaacaagag atgcagctcc
      361 ttacttcaga gtagctggca ggttaatgag tcttgactca gagccattga gcaagcaggc
      421 aattgcccag gcagtagaac ctaccttgaa tgatagaatc aaaaatcaat atgaagaaaa
      481 gggttatgta gattatgcct atgaaccaga tattggagca aaagtcaggt ataggataga
      541 actgtacaag tcaagaagtt gtatgaccgt tgctctgaga agagtcaaat cagaaattcc
      601 tacatttgaa gagttaaatc tgccccgggt atatgaaaag gtaatgcacc tgagaccaaa
      661 aggtattatt gtgataggtg gagagacatc gagcggaaaa tccactacat tagcggctat
      721 gatggattac attaatcaaa atgagaaaaa gcacatcgtc acaattgaag accctattga
      781 atatcttatc gttaataaaa tgtcatggat agaccagaga gaattcggtg gggactttca
      841 tagttatgca gacgcattaa gagctgttgt caggcaagat cccgatgtta taatgatagg
      901 tgaaatgcga gatcgtgata cggttgaagc agcaattgcc gcagctgaaa caggccatct
      961 tgtatttagc actctgcata ctacaacagc tactcagacc ttctacagaa tacttaattt
     1021 ttttcctccg gatcaaagat atgcagtaag gcagaatctg agtgccacat taatcgccat
     1081 aatgaatcag atgctattgc cttgcaaaaa acaaggtacg cgaatcatcc ctgcagcaga
     1141 agtccttatt aatacacctg ctgtaaagat gtacatcgaa aaaagcgaag aaaacaggtt
     1201 gccggaagtg atggaaagcg gaacaaaaga cggcatg
//
LOCUS       DBNGSV010000289         2660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_536360, whole genome shotgun sequence.
ACCESSION   DBNGSV010000289 DBNGSV010000000
VERSION     DBNGSV010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_536360"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1641)
                     /locus_tag="AC159U_10640"
     CDS             complement(40..1641)
                     /locus_tag="AC159U_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3337546.1"
                     /translation="MVKSKKITQNKQLSREIGFVALLFTGITGMIGSGWLFASMYAAQ
                     IAGPAAIFSWFIGGFVAIILALVYAELGGMLPVAGGIARIPHFSHGTINSFMAGWLCW
                     IAYVTTAPIEVTAVLEYSSNYFPWLTINEHGERALSKEGVFVASILMLIFVIINMRGV
                     KWLANANTTITFWKLSVPVIAPIVLIIVGFRMENFHDFGGFAPNGVAGVLGAVSSGGV
                     MFCLFGFRTAMDMAGEVKNPQRNVPLAMIGAVVVSLIIYIILQVAFIGVIPPDHLQGG
                     WAKISESVPGGPFAAFATILGVQWLAIALYSDAILSPSGTGLAYTGATARINYAMSKN
                     RQIPRIFEKLNRFNVPIWSLVFNFIAGMLIFLPFPGWSELVGFISSSVVLSFAFGPVS
                     LAALRYQIPELHRPFRIPYATVFSAVSFIFVGFVIYWTGWETNWKVFLAALMGFIILG
                     ITRMTNKESADMLNLKQSLWFLPYIVGLGVISYLGSYGKGLGIINEAVAMAIITIFSL
                     AIFVLALKLRLPDDQARRFVSIEEQ"
     gene            complement(1735..>2660)
                     /locus_tag="AC159U_10645"
     CDS             complement(1735..>2660)
                     /locus_tag="AC159U_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3337547.1"
                     /translation="RLIQGVSVGGELVGSISFITEIAPKNRRGFFGSFSVCSAVGGIL
                     LGSAVATIAHGVFDAEFLNNWGWRIPFLLGFAIGGFGLWMRKGMVETPQFEKVKETGD
                     VGKSPVIEAVKSMPGMIAHVVAMLVLYAGGFYMLFVWWPTYLTTIVVPPISHALMINT
                     ISMVALMFLIPAAGYLSDLIGRKTVLGAAFLGMAVLAYPLVIWTDHGVFSSALISQLI
                     FAILIGGALGPMAATLVELFPTRIRFSSIGVSYNIALAIFGGTAPLVSTWLVSRTGDI
                     ESPAYYLIAMAVISLIATLTISKKLGGELED"
BASE COUNT          839 a          651 c          531 g          639 t
ORIGIN      
        1 cacttggaat ttagaagttc ttataattga ttaaaaaaat tactgttcct ctatactcac
       61 aaatcttctt gcctgatcgt cgggtagacg aagcttgagg gcaagtacaa aaatagcaag
      121 cgagaaaatg gttattatcg ccatggctac tgcttcgttt ataatcccaa gaccttttcc
      181 atagcttccc aggtaggaga ttacgcccag ccccacaatg tatggcaaaa accataggga
      241 ctgctttaag ttgagcatgt cggcagattc tttattagtc atgcgtgtta taccgagtat
      301 tataaagccc atcagggctg caagaaaaac cttccaatta gtctcccagc ctgtccagta
      361 aataacaaat ccaacaaaaa taaaagaaac ggctgaaaaa acagtagcat atggtatacg
      421 aaacggccta tgcagctctg gaatttgata cctgagtgca gcaagagaaa cagggccaaa
      481 agcaaaagac agtacgaccg aagagcttat aaaaccaaca agctcagacc atcccggaaa
      541 gggaagaaat atgagcatac cggctatgaa attgaacacc agactccata tcggcacatt
      601 aaaacggttg agcttttcaa agattcttgg gatctgcctg ttcttagaca tagcatagtt
      661 gatacgtgct gtagctccgg tgtaagcaag tcctgtgcct gacggggaaa gtattgcatc
      721 actatataga gcaattgcaa gccactgcac acccagtatt gtggcaaatg ctgcaaatgg
      781 ccccccgggt acactctctg atattttggc ccagccaccc tgcagatggt caggcggaat
      841 tacacctata aacgctacct gcagaattat gtatatgata agactaacta ctacagcccc
      901 aatcatggcc agagggacgt tacgctgcgg gttttttacc tcacctgcca tgtccatagc
      961 ggtacgaaag ccaaagagac aaaacatcac tccaccactg gaaaccgccc ctaatactcc
     1021 ggcaaccccg tttggagcaa accccccaaa gtcatgaaaa ttctccatgc gaaatccaac
     1081 aataataagt acaatagggg caataacggg tacggataat ttccaaaagg tgattgtagt
     1141 attcgcgttt gcaagccact ttaccccgcg catattaata atcacaaaaa taagcatcaa
     1201 gatactagct acaaagaccc cctcttttga aagtgccctc tcgccatgct cgttgatggt
     1261 aagccacgga aagtagttag atgagtactc taaaacagcg gttacctcta taggtgccgt
     1321 ggtaacatat gctatccagc acaaccaccc ggccataaaa ctgttgatcg ttccgtgaga
     1381 gaaatgcggt atacgcgcga taccccctgc tacggggagc atccctccca gctccgcata
     1441 aaccagcgca agaatgattg cgacgaaacc gcctataaac catgagaaaa tcgcagccgg
     1501 gccggcgatc tgagcagcat acatagaagc aaagagccac cccgagccta tcatccccgt
     1561 aatccctgta aaaagcagtg caacaaaccc tatttcacgg ctgagctgtt tattttgagt
     1621 gattttctta cttttaacca tttttcactc ttctcatttt gaagacaaac cagatcagta
     1681 atgaatagtg atttttatat atttacaata aattgtagtt attctttgat tcaatcagtc
     1741 ctcaagctcc ccacccagct ttttactaat tgttaaagta gctatgagac tgataactgc
     1801 catggctatt aaatagtagg caggggactc aatatcccct gtacgcgata caagccacgt
     1861 gctaacaagc ggagcggtgc ctccgaaaat agcaagtgca atattgtatg aaactccgat
     1921 gctgctaaat cttattcttg tcgggaaaag ttctacaagg gttgctgcca ttggacccaa
     1981 ggctccacca atgagtatgg cgaagattaa ctgagagatt agggcagagc tgaatactcc
     2041 atggtcagtc catattacaa gtgggtaagc aagtactgcc atacccaaaa acgccgctcc
     2101 aagcacagtt ttacgaccaa ttaagtctga gaggtatcca gcagcaggta ttaaaaacat
     2161 aagcgccacc atgctaattg tatttatcat tagtgcatga gatattggag gaacaactat
     2221 ggtggttagg tatgtaggcc accagacaaa gagcatataa aatcccccgg catacagcac
     2281 aagcatggct actacatgag cgatcatgcc cggcatagac tttacagctt caataacagg
     2341 actttttccg acatcacctg tttctttcac cttttcaaac tgaggagttt ccaccatgcc
     2401 ctttctcatc catagcccaa accctccaat tgcaaaccca agcaaaaacg gtatacgcca
     2461 tccccagtta ttaagaaatt ctgcatcaaa cactccgtgc gctattgttg caacggcaga
     2521 gccaagaaga atacctccga ctgcgctgca tacagaaaaa ctgccaaaaa aaccgcgtct
     2581 gtttttaggg gcgatctcgg ttatgaaaga aattgaacct acaagttctc cacctacaga
     2641 tactccctga ataagtcgta
//
LOCUS       DBNGSV010000290         3048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_675415, whole genome shotgun sequence.
ACCESSION   DBNGSV010000290 DBNGSV010000000
VERSION     DBNGSV010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_675415"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC159U_10650"
     CDS             complement(<1..670)
                     /locus_tag="AC159U_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3337548.1"
                     /translation="MENSETIPELLGNLTKKYKSRLLVQRRDGWSWKQITWLDFESEI
                     KNMASFLLSLGFESGDNAIVISSNNNECFVSELAIYSLGGTVIPFSNYDSSSIYSKSV
                     SKLKPKFVFVENQNFLTELKSNSDIYNKSEKIFIFDDSKVGDDDKVVPYMAAIKFGQI
                     KKKELSDKLKDVIQSISPDKIALRIYSGNTNSITETVFDHKTILEMLSRACERLKFIS
                     SEDQS"
     gene            748..2109
                     /locus_tag="AC159U_10655"
     CDS             748..2109
                     /locus_tag="AC159U_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR3337549.1"
                     /translation="MKKVIKLKPDNSSLYIEAKKIVELLRSKGKKAFIVGGSVRDTLL
                     GFEPKEYDITTSATPEEVCSYFKNTVEVGASFGVIIVLVDGVKFEVATFRKEESYSDG
                     RHPDEVQYSTEESDDVIRRDFTINGLLYDPEKEQVIDYVDGLADLEKKVIRTIGDPVD
                     RFREDKLRMMRAVRFSCRLDYEIDEPTDKALKEHAEAIVEVSTERVRDELIAIITQNN
                     PGKGLMALTNYGLLRHIMPEIEIMKGVEQPPQFHPEGDVFVHTCLVLDKMYENSDGIV
                     SPELALSGLLHDVGKPPTFTVSDRIRFNGHDRVGAAMSKKICRKLKLSNKQIQRIESL
                     VREHLKFKDVFNMRDSTFKRFVAMDYFDDHMALHKADCEASHGMMGAYEFIINKRSEF
                     TEDQIKPEPLINGDDLIRLGFKPGPIFSKILNSVEEMQLEGNITNKDQAIDYVRDNYA
                     EAR"
     gene            complement(2110..2535)
                     /locus_tag="AC159U_10660"
     CDS             complement(2110..2535)
                     /locus_tag="AC159U_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337550.1"
                     /translation="MRFKTVISFMVLVVICSSCYSRGKFAQSAQHLQEATERYYQVLK
                     WKYYDRAKVFIDPEDKALYEDFVLRLEEDLNFTNYEIKGYVYSEDKKECEVKVAITYY
                     TYPSVSEKRDVLYEKWVERGNTWYVKPDFSHEFYQSPDK"
     gene            complement(2627..2950)
                     /locus_tag="AC159U_10665"
     CDS             complement(2627..2950)
                     /locus_tag="AC159U_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3337551.1"
                     /translation="MNKLFAFMSVMVFAVLAMVAPDAHAAEGGGGDLGKLGLAIGAGL
                     GIGIAAGVAGLGQGRATAAALTGIARNPGAASKIQTPMIIGLALIESLVIYALAITFL
                     LQSKI"
BASE COUNT         1009 a          559 c          592 g          888 t
ORIGIN      
        1 atgactgatc ctctgatgaa ataaatttaa gcctctcaca agcccgagat agcatttcga
       61 gtatagtttt atgatcaaac acagtctcag taatactgtt ggtattacct gaataaattc
      121 taagtgctat cttatcggga ctaattgatt gtattacatc ttttaattta tcagataatt
      181 ctttcttttt gatctgtcca aattttatag cagccatata tggaacaact ttgtcatcat
      241 ccccaacctt cgaatcatca aaaataaata ttttttctga tttattgtat atatcggaat
      301 tacttttaag ctctgttaaa aagttttgat tttcaacaaa cacaaatttt ggttttaatt
      361 tacttacgga tttgctataa atagaagatg aatcgtaatt gctaaaagga ataaccgtgc
      421 cgcctaggga ataaatcgca agctcactaa caaaacactc attattgtta gatgaaatta
      481 caatggcgtt atcgcccgac tcaaacccaa ggcttaaaag aaacgaagcc atatttttga
      541 tttcactttc aaaatcaagc caggtgatct gcttccagct ccagccgtca cggcgctgta
      601 ccagaagcct gcttttgtat tttttggtta aattccctaa aagctcaggg atagtctcag
      661 aattttccat ttatttatat ttcaattact ttattgttat caggtaaatt ataaaagagt
      721 tatagtatta cctaaaatat ttaaaaaatg aaaaaagtaa taaaattaaa gccagataat
      781 agcagcctct atattgaagc caaaaagatt gtagagcttt tgcgctccaa agggaaaaaa
      841 gcgtttatcg tcggaggctc agtacgagac acccttttgg gttttgagcc aaaagagtac
      901 gacattacca caagtgcaac tccagaggaa gtttgcagtt attttaaaaa tactgtagaa
      961 gtaggagcca gttttggagt aataattgtt ctcgttgatg gagtaaaatt tgaggttgct
     1021 acttttagaa aagaggagag ctactccgac ggcaggcacc ccgatgaagt gcagtacagc
     1081 acagaagaat cggatgatgt gataagaaga gacttcacga tcaacggact actctatgac
     1141 ccggagaaag aacaggtgat agattatgta gacggccttg ccgatcttga gaagaaagtc
     1201 ataagaacca ttggcgatcc ggttgataga tttagagaag acaaactgag gatgatgcgt
     1261 gctgtgaggt tttcatgcag gcttgattat gaaattgacg agcctactga caaagcgctc
     1321 aaggagcatg cagaagcaat tgtagaggta agtacagaga gagtaaggga tgagcttata
     1381 gcaattatta ctcagaacaa ccccggaaaa ggccttatgg cattgaccaa ctatggttta
     1441 ctaaggcaca ttatgccaga gatagaaata atgaagggag ttgagcagcc accgcagttc
     1501 caccctgaag gagacgtttt tgttcatact tgcctggttc tggataagat gtatgaaaat
     1561 tcagatggaa ttgtaagccc cgagcttgca ctctcaggct tgcttcatga cgtaggaaaa
     1621 cctcccactt ttaccgtttc tgacaggata aggttcaatg gtcatgacag agtgggtgct
     1681 gcaatgtcta aaaaaatatg cagaaaacta aaactatcaa acaagcagat acagagaatt
     1741 gaatcgctcg taagagaaca tctaaagttt aaagacgtat ttaacatgag agacagcaca
     1801 tttaagcgct ttgtagccat ggactacttt gatgatcata tggcgcttca taaggcagac
     1861 tgtgaggcaa gccatgggat gatgggagca tatgaattta ttataaataa gcgctcagaa
     1921 tttactgaag atcagataaa acccgagcct ctaatcaatg gggatgatct cattagactt
     1981 ggatttaaac cagggccaat attttctaaa attttaaaca gtgtagaaga aatgcagctt
     2041 gagggaaata taacaaataa ggatcaggct atagattatg tgagagataa ttatgcagaa
     2101 gctaggtaac tatttatctg gtgactgata aaactcatga gagaaatcgg gctttacata
     2161 ccatgtattt cccctctcaa cccacttttc ataaagcaca tctctttttt cagatactga
     2221 agggtatgta tagtaggtta tggcaacttt aacttcacac tcttttttat cttcagagta
     2281 tacatagcct tttatctcat aatttgtaaa gttgagatcc tcttcaagcc tcagaacaaa
     2341 gtcttcgtat aaagctttat cttcagggtc tataaatacc ttggctctat catagtactt
     2401 ccattttaag acctgataat atctctcagt ggcttcttgc agatgttgtg cactctgggc
     2461 gaatttaccc ctcgaatagc aggaagaaca aataactaca agtaccataa aagagattac
     2521 agttttaaat ctcatatttt aatataacac tactattcag gtatgggaaa ccggtaaata
     2581 gaaattaagg ggagtgccaa atgcactacc cctaaatttt atgtgtttaa atttttgatt
     2641 gtaataaaaa tgtgatagcc aaagcataga tcacaagtga ctcgataaga gcaagaccga
     2701 taatcatagg tgtctgaatc tttgaagctg cacccgggtt tcttgcaata ccggtaagag
     2761 ctgctgctgt tgctctaccc tgtccaagtc ctgcaacacc tgctgctatt ccaataccaa
     2821 gaccggcacc gatagcaaga ccaagtttac caaggtctcc gccaccacct tctgctgcgt
     2881 gcgcgtctgg tgctaccata gcaagtactg caaaaaccat aacactcata aaggcaaata
     2941 acttattcat gcttacctcc tcctattaat gtaattttat ttttagtgtt catgtgcaat
     3001 agaaagtgaa atatatattg atgataaaat agtgaaaata aatgcctg
//
LOCUS       DBNGSV010000291         1336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_813442, whole genome shotgun sequence.
ACCESSION   DBNGSV010000291 DBNGSV010000000
VERSION     DBNGSV010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_813442"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="AC159U_10670"
     CDS             <1..634
                     /locus_tag="AC159U_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3337552.1"
                     /translation="AQKTINEKISLPKGYKLEWAGQYEYLQRVKEKLSYVVPVTILII
                     FILLFLNFNSLIESLIVLLSVPFSLVGSIWFLSILGYNLSIAVWVGIIALAGLAAQTG
                     VVMIIYLDEFYDKMVAEGKVSLKNLEQAIHDGAVQRVRPKMMTVMALIMGLLPLMWSH
                     GTGADVMKRIAAPMVGGLVTSTILTLVIIPVIYAIWRGRELDKHIEKTKL"
     gene            653..>1336
                     /locus_tag="AC159U_10675"
     CDS             653..>1336
                     /locus_tag="AC159U_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3337553.1"
                     /translation="MKYTCPMHPEIIEDKPGNCPKCGMSLEKATPSLDTGVKYVCPMH
                     PEVVRDEPGDCPICGMALEKQVVTLGDEEENEELKDMSRRFWVSVILSVPIVIIAMSH
                     LIQSDYLKNLFASKVFQYVELVLATPVVLWCGWPFFVRAYKSVVNKSLNMFTLIGLGV
                     AVAYIYSLIAALFPEIFPPSFRGLGGKVGVYFEAAAVIVTLILLGQVLELKARSQTSS
                     AIKALLGLAP"
BASE COUNT          382 a          236 c          324 g          394 t
ORIGIN      
        1 agcacaaaaa actattaatg aaaaaatttc acttcctaaa ggctataagc tcgagtgggc
       61 cggtcagtat gaatatttgc aaagggttaa agaaaaatta agttatgtgg ttcctgttac
      121 gattctaata attttcattc tgctatttct caattttaac tcgttaattg agtcattgat
      181 tgtactcctg tcggtaccgt tttctctggt aggaagcata tggtttctaa gtatcctcgg
      241 atacaattta agtatagctg tctgggtagg aataatagcc cttgcaggtc ttgccgctca
      301 gacaggagta gtgatgatca tatatttgga tgagttttat gataaaatgg ttgccgaagg
      361 aaaggttagc ctgaaaaatc tcgagcaggc catacacgat ggtgcggtgc aaagagttag
      421 gccaaagatg atgacggtga tggctcttat tatgggtcta ttaccactta tgtggagtca
      481 tggcacggga gctgatgtta tgaaaagaat tgcagcacct atggtaggcg ggcttgttac
      541 atcaactatt cttactcttg taataatccc tgtaatatat gcaatttgga ggggcaggga
      601 attagataaa catatagaga aaactaaatt ataattttaa agggtaaaaa atatgaaata
      661 cacctgcccg atgcaccccg agattattga ggataaacca ggcaattgtc caaaatgcgg
      721 tatgtcactg gaaaaagcaa ctccgtctct tgataccgga gttaaatatg tctgccctat
      781 gcatcctgag gttgtaaggg acgagccggg ggactgtcct atatgcggaa tggctttaga
      841 gaagcaggtt gtaactttag gtgacgaaga ggaaaatgaa gaattaaagg atatgagcag
      901 acgcttttgg gtaagcgtga tcttgtcggt tccaattgta ataatcgcga tgagccactt
      961 gatacagagt gattatctta aaaatttatt tgcttcgaaa gttttccaat atgttgagct
     1021 tgtccttgca accccggttg tgctctggtg cggctggccg ttttttgtaa gggcatataa
     1081 atccgtagtc aacaaaagcc taaacatgtt tactctcata ggccttggag ttgcagtagc
     1141 gtatatatac agcttaattg ccgctctttt tccagagata tttccccctt cattcagagg
     1201 gctcggcggg aaggtggggg tgtattttga ggctgcagct gtaattgtga ccctcatact
     1261 gcttgggcag gtactggagc taaaggcaag gtcacagaca agctccgcaa ttaaagcact
     1321 cttgggtctg gctcct
//
LOCUS       DBNGSV010000292         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_536878, whole genome shotgun sequence.
ACCESSION   DBNGSV010000292 DBNGSV010000000
VERSION     DBNGSV010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_536878"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="AC159U_10680"
     CDS             complement(<1..919)
                     /locus_tag="AC159U_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HIR3337554.1"
                     /translation="MAEQSLTLGIQGNKDVAAITNSLNSLKIALASLPPEISKVSASL
                     QNLDRSLEAAGKEQTNLANSSKNIKTSNESMFSSFLKVNVVMKAFNLAMQTLKMAFVD
                     SIKSALEFNKVFANVTTLVDTNVVNVGKMQKELLQLDNRLGSATELTKGLYQALSASV
                     APAEAVKFVGEAAKFARAALTDTETAVDVLTTTLNAYGMATSEVTHVSDVFFQTIKLG
                     KINGEQLASNLGTVIPLAANLGINIEELGAAMAVFTKQGINAANAATRINATMTAFLK
                     PSEAMIGALQKLGFESGSQAVKDLGGLKNA"
     gene            complement(935..1210)
                     /locus_tag="AC159U_10685"
     CDS             complement(935..1210)
                     /locus_tag="AC159U_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337555.1"
                     /translation="MGVRGVFANSFKRKYVHAFNTVYFHSWKQVWWFYHDKGLLPYAN
                     KGWYEHNKKIVRVIEILENLYAHYQNEVETKSTEAINREIERKRRKK"
     gene            complement(1288..1740)
                     /locus_tag="AC159U_10690"
     CDS             complement(1288..1740)
                     /locus_tag="AC159U_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337556.1"
                     /translation="MSNENKDVNIEDLNSYVFDINDEATTYTLDCFKNKERPVEKQMR
                     VSIEPLSAKEANEITKEAYASTGQRNKMSEEEWTGKYSTHFTRISFTKKIKKLENVFF
                     RLKDGTIKEIIDPIELWGIKDKRAAEIVSEIQKLMNSQDEVSEEEEKN"
     gene            complement(1838..>2089)
                     /locus_tag="AC159U_10695"
     CDS             complement(1838..>2089)
                     /locus_tag="AC159U_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337557.1"
                     /translation="LTRIEQEPDGASTNIARSNNTPIYFVGVLQKDQTVGEKVIAIVG
                     KVELEGFTYGATDGSAQEFTSGFSPTAGSTIQKVVVNIV"
BASE COUNT          599 a          441 c          315 g          734 t
ORIGIN      
        1 ctgcattttt cagtccgcct aaatctttaa ctgcctgaga gcccgattca aatcctaatt
       61 tttgcaatgc tccaatcatg gcctcagaag gttttaggaa tgccgtcatg gtagcattga
      121 ttcgcgtagc tgcatttgcg gcattaatac cctgctttgt aaaaacggcc attgccgcac
      181 ccagttcttc tatattaata ccgagatttg cagccaaagg tattacagta ccaaggttag
      241 aggctaattg ctcaccattg atcttaccta gttttattgt ttgaaaaaat acatcactta
      301 catgggtaac ttcacttgta gccataccat aagcattaag agtagtggtt aatacatcaa
      361 ctgccgtttc ggtatcagtc agagcggccc ttgcaaactt tgccgcttcc cctacaaatt
      421 taacagcttc tgccggggcc acacttgcag acagggcctg ataaagtcct tttgttaatt
      481 cggtcgcact tcctaagcgg ttatcaagtt ggagtaattc cttttgcatt tttccgacat
      541 taacaacatt cgtatcaacc aaagttgtaa catttgcaaa caccttatta aattctaatg
      601 ctgattttat actgtccaca aaagccattt tcaaagtctg catagcaaga ttaaaagctt
      661 tcataactac attaactttt agaaatgaag agaacatgct ttcattactt gtttttatat
      721 tcttgctcga gtttgctaaa ttagtttgtt cttttccagc tgcctctaaa gaacggtcta
      781 aattttgtaa agacgcactc actttagaaa tttcaggagg taaagaggct aaagcaattt
      841 ttaagctgtt taaactattt gtaatagcag ctacatcttt attaccttga atacctaagg
      901 ttaagctttg ctcagccatt gtctacctcc attattattt ctttcttctt ttacgctcaa
      961 tttctctatt tatagcttct gtagattttg tttcaacttc attctgataa tgagcatata
     1021 agttttctaa tatttcaata actcttacta tttttttatt gtgttcatac cagcctttat
     1081 tggcataagg taataagcct ttatcgtggt aaaaccacca aacctgcttc cagctatgga
     1141 aatacacagt attaaaagca tgcacatatt tgcgcttaaa actattagca aaaacaccgc
     1201 gcacgcccac gatgtaaatg gtatctcctg agaatggccc tacctctata ttaaaacctc
     1261 cttccaacca tagaagaaag gaggtcttta gtttttttcc tcttcctcac ttacttcatc
     1321 ctgggagttc ataagtttct ggatttcaga tacaatctcg gcggctcttt tatctttaat
     1381 accccatagt tctattgggt caattatctc tttaatagta ccatctttca gtctgaagaa
     1441 gacattttca agtttcttga tttttttggt aaaagaaatt cttgtaaaat gagttgaata
     1501 tttacctgtc cactcttctt cactcatttt attgcgctgc ccggtagatg cataagcctc
     1561 ttttgtaatt tcatttgctt cttttgccga taaaggctca atagaaacac gcatttgctt
     1621 ctcaaccggt ctttctttat tcttgaaaca atctaaagta taagtagttg cttcgtcatt
     1681 aatgtcaaaa acataagaat ttaaatcttc aatattaaca tctttatttt cgttactcat
     1741 tagtatttat ccttactata tttagtttta ttctctatac agagaaatgt aaaaaaagta
     1801 ctgcgtacat ataaaataca cagtacttaa atgccgatta aacaatatta actactacct
     1861 tttggattgt agaccctgcc gttggtgaaa aaccacttgt gaattcctga gcacttccgt
     1921 ctgtagctcc ataggtgaaa ccttccaact ctacctttcc tacaatagca atgactttct
     1981 ctccgactgt ctggtctttt tgtaaaacac ctacaaaata tataggagta ttattagatc
     2041 ttgcaatatt agtagaggcc ccatccggct cttgctctat acgagttaa
//
LOCUS       DBNGSV010000293         5597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_675973, whole genome shotgun sequence.
ACCESSION   DBNGSV010000293 DBNGSV010000000
VERSION     DBNGSV010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_675973"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1250
                     /locus_tag="AC159U_10700"
     CDS             45..1250
                     /locus_tag="AC159U_10700"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HIR3337558.1"
                     /translation="MKYVILQGDGMPDHKLAELGGKTPLEVADTPNLDYIAKYAGTFG
                     MVKTIPDNYPPGSDVGNLTVLGYDPKKYYTGRSPLEAASIGVELKPTDVTLRCNLVTL
                     SGNTGSEIMEDYSAGHITTEEAHEIIKDIKAELDGDGFTFHPGISYRHLLVWSDGNKD
                     VITTPPHDISGKKISPHIPVGQGADLLLELIKKSQDVLKDHPVNNKRIEQGQNPVTSI
                     WLWGQGTAPAMPSFKELYGLSGSVISAVDLVKGIGHYAKLDVIDVPGATGYLDTNYEG
                     KVEYALNSLKNVDITMIHIESTDETGHVGDAKLKIQAVEDFDKRVVAHVLEGIKKFGD
                     YRILVMSDHPTPIDIRTHTSDPVPFAILSSDDTEIRNDSYVYTEDCAASTGEFEDEGW
                     KLLGRLIGK"
     gene            complement(1274..1795)
                     /locus_tag="AC159U_10705"
     CDS             complement(1274..1795)
                     /locus_tag="AC159U_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337559.1"
                     /translation="MSSLTQQRRDANYERLWNVVKEFGKVLETDSNSINLLGIRSETE
                     LGTPKKTIKRSLATLLLLEDNDSAIATLASAYLFIENFVDNRIYEYFLPKLKTMGEIR
                     VQSGDISTQEVQKLGAKVLEDLKDNEFQDNYQAIKAAMDKVRKSAENELHILLSLAER
                     DGLNSNKKKDLLV"
     gene            complement(1808..2572)
                     /gene="mazG"
                     /locus_tag="AC159U_10710"
     CDS             complement(1808..2572)
                     /gene="mazG"
                     /locus_tag="AC159U_10710"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR3337560.1"
                     /translation="MSKKFEDLVELARYLRSPEGCPWDKEQDLQKLKSYIIEEAYEAV
                     QAIESEDVDEITEELGDLFFQVIFAAQIGQEEKKFDIDVIINSLHNKLIRRHPHVFGE
                     KKAKNAEEAVKSWQTQKLKEKKRKRTLLEIPRDMPALLRAQRVGEKASNVGFDWQDSH
                     DVLQKVDEELNELKVEIKKGDAEGIEQEWGDLIFTLVNLGRHLKIDGESSAHQAVNKF
                     ISRFSRIEEKAKQQGKQLSELSLGEMDSIWNEIKSD"
     gene            complement(2569..3519)
                     /locus_tag="AC159U_10715"
     CDS             complement(2569..3519)
                     /locus_tag="AC159U_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3337561.1"
                     /translation="MDTITHGLIGSLGSKTGYYQKFGKIATYSFLFGSIFPDIDVIVA
                     ILGPDFTMRYHRGITHSLLAVPLFSILVTYITTLIFKTREYKKIYPMVLLGMYSHIFF
                     DLITAYGTVAFDPISIKRYNWNLVFILDPFITLPVIAGLIVSYKKRALAFKTSVIIFL
                     FLSSYLMTCYFVKEHNKNRIIEYTKTHNTQVEKLDIYPRPLAPFYWLGVVETKDSFYR
                     AQVSSLSKDSLIFESFSKSDVNGFVDAARDHRVAKLYFWFADFPVAKYLRSGDEHIVE
                     IVDLRFRMLSRRTPFTLRYVFDKTMKIKEITLGGRKVRNL"
     gene            complement(3523..4647)
                     /locus_tag="AC159U_10720"
     CDS             complement(3523..4647)
                     /locus_tag="AC159U_10720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3337562.1"
                     /translation="MNKAYLIIDTSEGNADLFYKTRFFVPDPCIYIEHKRKKILVLSD
                     LEIDRGREEATVDKLISFSEYIKKIPAAKRKKAGFADIVDLIFKERKIKSAEVPGRFP
                     IKYADQLRKLGYKIVPKKSEPFFEGRLKKHPHEVASLKQSLKWTADAMRVAVNIISLS
                     KAKKNRLYFNGKVLTSEFIQGEINSFLSKHGYTASHTIVASGVQGSMPHHSGSGPIIS
                     GTPVVIDIFPRSQKTGYFGDMTRTIIKGEPSKELKKMYSVVLKGQKIGIDMIKHGVNS
                     KDVHNAIVDYFDKSGFKTAVIDGKQQGFIHSTGHGLGLEIHELPRVSKGDDVLEAGNV
                     VTVEPGLYYEKLGGIRIEDVVLVTRTGCEVLSKFPKKFQV"
     gene            4879..>5597
                     /locus_tag="AC159U_10725"
     CDS             4879..>5597
                     /locus_tag="AC159U_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3337563.1"
                     /translation="MARPVRLEFKNAVYFISSKSSGRKHIVKDDADRQTFLDTLEYVV
                     NRYRWKCHAYCIGNTEYNIAIETPRGNLSVGMRQLNGIYTQKFNRKNKKSGNVFQGRF
                     KAVVVQKDKYLLDICRHVVTLSQREGITNNINTFKWSSYRATSGTVDVPEFLHVEWIH
                     SQFAKRTKNAQDKYRDYIKGANKAENPLDSVRGQILLGDEQFIEQMTLMLIKGRNKED
                     VPKWQRHIKRPPLEEIFKGIET"
BASE COUNT         1752 a         1097 c          983 g         1765 t
ORIGIN      
        1 atatctctct tgattcatct caaaaaccat ttaacatctg ctctatgaaa tatgtaatat
       61 tacagggcga tggtatgccc gaccacaagc ttgctgaact tggcggaaag acaccactgg
      121 aagtagcaga tacgccaaat cttgattaca ttgctaaata tgcaggaaca ttcgggatgg
      181 ttaagactat tcccgataac tatccacccg gaagtgacgt gggtaacctc actgtacttg
      241 gctacgatcc gaaaaaatac tacaccggca gatccccact tgaagctgca agtataggtg
      301 ttgagcttaa accaacagat gtaaccctaa ggtgcaacct agtaacactc tcaggaaata
      361 ccggctcaga aatcatggaa gactacagtg cggggcatat taccactgaa gaggcgcacg
      421 aaataattaa agatattaaa gcagagttgg atggtgacgg ttttacattc catccgggaa
      481 taagctacag acatctactt gtctggtcag atggaaacaa ggatgttatt acaactccgc
      541 cacatgatat ttcaggaaag aaaattagtc cccacattcc cgtaggtcag ggtgctgatc
      601 tcttattaga attaatcaaa aagtcacaag atgttttgaa agatcatcct gtaaacaata
      661 agaggattga gcaaggacaa aatcctgtta caagcatctg gctctgggga caggggaccg
      721 cacccgctat gccaagcttt aaagagcttt atggactctc gggttcggta atttctgcag
      781 tagatcttgt aaaaggtatt ggacattatg ccaagctgga tgtgattgat gttccgggtg
      841 ccacggggta tctggataca aactatgagg gaaaggtgga atatgcacta aattctctaa
      901 aaaatgttga cattactatg atacatattg aatccacaga tgaaacgggc catgtgggtg
      961 atgcaaagtt aaaaattcag gcagtagaag attttgataa acgtgttgta gctcatgttt
     1021 tagaaggcat aaaaaaattt ggagactaca ggattttagt gatgtctgat catccgactc
     1081 caattgatat aagaacccat accagcgatc cggttccttt tgcgatatta agctcagatg
     1141 atacagaaat cagaaatgat agttatgttt acacagaaga ctgtgccgca agtaccggcg
     1201 agtttgaaga cgaaggatgg aaacttctgg gcagattgat tggtaaatag atcttttatg
     1261 ctgctttaac aattcagact aataaatctt ttttcttatt agagttcaaa ccatctcttt
     1321 cagcaaggct aagcagtata tgaagctcgt tttcagcaga ctttcgcacc ttatccattg
     1381 cagcttttat agcctgatag ttatcctgaa attcattgtc ctttaagtct tccagtactt
     1441 tagcgccaag tttttggact tcttgggtag aaatatcacc tgactgtact cttatttctc
     1501 ccatggtttt tagttttggc agaaaatatt cataaatcct gttatccaca aaattttcta
     1561 taaatagata tgcagaggcg agtgtggcaa ttgcgctgtc attatcttca agcaataata
     1621 aagttgcaag cgatctctta atggtctttt tgggtgttcc aagctcagtc tcagatctta
     1681 taccaagtaa gtttattgaa ttagaatccg tttctaaaac cttgccaaat tctttaacta
     1741 cattccataa cctctcataa tttgcatcgc gtctttgctg tgttagtgag ctcattttaa
     1801 tatcccgtta gtctgatttt atttcattcc aaatactgtc catttctcca agcgaaagtt
     1861 cagataactg ttttccctgt tgtttggcct tttcttcaat ccttgaaaac ctggaaatga
     1921 atttgtttac tgcctgatga gcagaacttt ccccatcgat tttcaagtgc ctgccaagat
     1981 ttaccagagt aaatataagg tctccccact cctgctctat tccctcagca tctccctttt
     2041 ttatctctac ttttagttca tttaattctt catctacttt ttgcaaaaca tcgtgggagt
     2101 cctgccagtc aaagcctaca tttgaagctt tttcaccaac cctctgtgct cttagaagag
     2161 cgggcatatc tcttggaatt tcaagcagtg ttctttttct ctttttttct tttaattttt
     2221 gtgtctgcca gcttttaact gcttcctctg catttttagc ctttttttca ccaaaaacat
     2281 gaggatgtct tcttataagc ttgttatgaa gactattaat tataacatca atatcaaatt
     2341 tcttctcctc ctggcctatc tgtgcagcaa atatcacctg aaaaaacagg tctccaagct
     2401 cttcggttat ctcatctaca tcctctgatt caattgcctg aacagcttca taagcctctt
     2461 caatgatata ggatttcagc ttttgtaaat cctgctcttt gtcccaggga catccttcag
     2521 ggcttctgag gtatcttgca agctccacca gatcttcaaa ttttttgctc ataaattcct
     2581 gactttcctg cctccaagag taatctcctt tatcttcata gtcttatcaa acacatatct
     2641 aagtgtaaat ggtgtccttc ttgagagcat cctgaacctt aaatcaacaa tttcgacaat
     2701 atgctcatct ccgctcctaa gatatttggc aacaggaaaa tcagcaaacc agaaatacag
     2761 tttagctacc ctgtgatccc tagcggcatc tacaaaacca tttacatcag attttgaaaa
     2821 tgattcaaag ataagagaat ccttggagag tgaagaaact tgtgccctgt aaaagctgtc
     2881 tttggtttct accaccccaa gccagtaaaa tggggctagg ggtcttggat aaatgtcgag
     2941 tttttctacc tgcgtgttat gtgttttagt atattcaata atcctgtttt tgttgtgttc
     3001 ttttacaaaa taacaagtca tcagataaga gcttagaaac aaaaatatga taaccgatgt
     3061 tttaaatgct aatgctcttt ttttatagga gacgatcagg cccgcaatca ctggaagtgt
     3121 aataaacgga tcgagaataa aaaccagatt ccaattatat cttttaatac ttatgggatc
     3181 gaaagcaacc gttccatatg ctgttatcag gtcaaaaaat atgtgtgaat acattcctag
     3241 aagcaccatg gggtatatct ttttgtattc acgggtttta aaaataagag ttgtaatgta
     3301 tgtaaccaag attgaaaaaa gtggtacagc taataatgaa tgagtaatcc cgcggtgata
     3361 gcgcattgta aaatcaggcc ctaaaatagc cactattaca tcaatatccg gaaaaatgga
     3421 tccaaataga aaagaatagg tcgcaatttt tccaaatttt tggtagtagc cggtttttga
     3481 accaaggctt cctataagtc cgtgagtaat tgtatccaaa atctaaacct gaaatttttt
     3541 gggaaattta cttaatactt cacacccagt tctagtaacc aggacaacat cctctatcct
     3601 aataccgcct agtttttcat aataaagccc tggttctaca gttaccacat ttccagcctc
     3661 gagaacgtca tctcctttac taactctcgg aagttcgtgt atttcaagcc caagtccgtg
     3721 acctgtggaa tggataaacc cctgttgctt tccgtcaata actgctgttt tgaaaccgct
     3781 tttatcaaaa taatccacaa tagcattatg aacatctttt gaatttacac cgtgctttat
     3841 catatctata ccgatttttt gtcctttgag cactacgctg tacatttttt ttaattcttt
     3901 agatggctct cccttgataa tggtgcgtgt catatcacca aaatagccgg tcttttgaga
     3961 cctggggaat atatctatca ctactggagt gcctgatatt atagggcctg agcccgaatg
     4021 atggggcata gaaccctgaa ctcccgatgc cacaatagta tgagaagctg tgtagccatg
     4081 ttttgataga aatgagttaa tttctccctg aataaattca gaagtcagaa ctttaccatt
     4141 gaaataaagc ctgttcttct tagctttcga cagacttata atattaacag ccaccctcat
     4201 tgcatcagca gtccacttaa gtgactgttt taatgaagca acttcatgtg gatgtttttt
     4261 taatctgccc tcaaaaaaag gctctgattt tttagggact atcttatagc ctaattttct
     4321 taattgatct gcatatttga ttggaaatct accaggtact tctgcacttt ttatctttcg
     4381 ttctttaaaa atcagatcta ctatatcagc aaaacctgct ttttttctct tagctgcagg
     4441 aatttttttt atatattcag aaaacgatat taatttatct accgtggctt cttccctgcc
     4501 cctatctatc tccagatcac ttagaaccaa tattttcttg cgtttatgtt ctatgtatat
     4561 gcatgggtcg ggtacaaaga aacgagtttt ataaaatagg tctgcattgc cttcagatgt
     4621 atctattatt aaataagctt tattcatgat atttacttta gttattaaca gcaatataat
     4681 tacattaagt tacaaagata tcataattaa tttcatatca aattaattgt acaaataaca
     4741 aaaaatattt taaaaaactc gtaaatttat gaaactaaat actgcattgt ataaaattaa
     4801 ccgtagcact aaaattttac taaatatttg ggggacagtt tttatttatt aaaaaatatc
     4861 tgataaaata tactcagtat ggcaagacct gtaagattag aattcaaaaa tgcagtttac
     4921 tttattagtt caaaatcaag cggcagaaaa cacattgtaa aagatgatgc tgaccgtcaa
     4981 acatttcttg acacactgga atatgttgtc aacagatacc gctggaaatg tcatgcatac
     5041 tgtataggaa ataccgaata taacatcgca atagagaccc ccaggggaaa cctatctgtc
     5101 ggtatgcgac aattaaacgg tatttatacc cagaaattta acagaaaaaa caaaaaaagc
     5161 ggtaatgtgt ttcagggaag atttaaggct gtagttgtac aaaaagataa atatttgctc
     5221 gatatatgca gacacgtagt cacattatct caaagagaag gtataacaaa caacataaac
     5281 accttcaaat ggagcagtta cagagcaact tctggtaccg tagatgttcc cgaattttta
     5341 catgttgaat ggatacactc ccagtttgct aaaagaacca aaaatgccca ggataaatac
     5401 agggattaca ttaaaggtgc caataaagca gaaaatccgc ttgatagcgt aagaggacag
     5461 atacttcttg gagatgagca atttattgag cagatgaccc ttatgctcat taaaggcaga
     5521 aataaagaag atgttcctaa atggcaaaga catataaaaa gacctccact tgaagaaata
     5581 tttaaaggaa tcgaaac
//
LOCUS       DBNGSV010000294         4373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_467485, whole genome shotgun sequence.
ACCESSION   DBNGSV010000294 DBNGSV010000000
VERSION     DBNGSV010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_467485"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..874
                     /locus_tag="AC159U_10730"
     CDS             239..874
                     /locus_tag="AC159U_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337564.1"
                     /translation="MELEQLIQSEENKDKEQLNLTLQIAEKEWNKIRESLSLCGDIGE
                     FSKEDFMVGIIEEDVIIRLPKLSPTKSVSVYAPTFYPMYFIKNIKAMSENFSKKGYRS
                     TQALYSFIELAAKAAERLGLRGTLSMAFGVGYANVRTGWIAEKGLQKEREIFEQMFFS
                     GKFKNYDWEGDWTSVKKVLKEIFDKFNSWQNDEALYKRESDPKKIVKPMLV"
     gene            complement(875..2224)
                     /gene="dnaB"
                     /locus_tag="AC159U_10735"
     CDS             complement(875..2224)
                     /gene="dnaB"
                     /locus_tag="AC159U_10735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3337565.1"
                     /translation="MELSRPLPQNLEAEQAVLGALIIEGSLINQVLEILTTEDFYKDS
                     HKKIISAMIDLDRDSKPIDLLTVFEFLKAKGQLLEDVGGSNYLTYLTEIVPTTANIGY
                     YAQIVKEKSILRNLVVTASDIAKESHEDGVDVDELVDRAEHSILEVGQNRVKPSFYNT
                     NMLASGALEMIEALSKRKEHLTGVPTGFERLDHMTSGLQPSDLIIIAARPGLGKTSFC
                     LNLASHSAIMNKKNIAFYSLEMTKEQLMLRMLSIQAKVSYSSIRSGYIGTKDLEKLVK
                     AADVYSKAGIYIDDTPAISSLELRAKARRLSKDKGLDLIIVDYLQLMRGSRRTETRER
                     EIAEISGSLKSLAKELSVPVIAISQLSRQPESRTDKRPQLSDLRESGALEQDADLVMF
                     IHRADAYKNKKDEKDGTAELIIGKQRNGPTGSIKLAFLESQGIPSFENLDEEHEDAF"
     gene            complement(2313..3638)
                     /locus_tag="AC159U_10740"
     CDS             complement(2313..3638)
                     /locus_tag="AC159U_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3337566.1"
                     /translation="MDFKKYRKQCIDIFHEALQAANPYKCVSDKLSIKNSKLKIDTKK
                     YDLDNFKNIYVIAFGKASTEMALAVEHSLGTYITDGIVVSNHDSPPAYKKLKYLKSSH
                     PIPDEKSMKAAKKAVELLEHSTKDDLVIFLISGGGSSMLALPAEGLSLHEKRNVTEML
                     LKSGVDTYGLNTIRKHISQIKGGGLLKKALPSQVATLILSDVVGDKLDGIASGPTVPD
                     ETTFKECWKVIEALRLEHRIPPQVVLHLERGAMGEIADTLKAKDIDQQKVQTVVVGSN
                     FKSLVSAKKQAKKLGYNSLLLSSQMNGEAREVAKAISAIAFEIEKYDLPIKKPACLIF
                     GGETTVTVEGSGKGGRNTETALSFSMEILGHNIIGLFCGTDGIDGPTDAAGAICDGNT
                     RARARKMDVSAREHITNNDSYHFFDHLGDLIITGSTGTNVMDLGIVLIP"
     gene            complement(3677..4150)
                     /gene="nuoE"
                     /locus_tag="AC159U_10745"
     CDS             complement(3677..4150)
                     /gene="nuoE"
                     /locus_tag="AC159U_10745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR3337567.1"
                     /translation="MPFSTELKAKITDNVNSAENKQSALIPVLHTVQDELGHISNESM
                     EEIAAILELSPSCVQNVVTFYTMYFNEPKGKHVIWLCRTLSCALRGAEQVEHYIGDKL
                     CVKTGETTADGKITFMEAECLAACGTAPVMLVDEQLHENLTKSKVDSIIEDLQKS"
BASE COUNT         1288 a          885 c          774 g         1426 t
ORIGIN      
        1 ctttgttttg tatctaaaag agatggaatt tagatttaat aacagaaatg tagatttagt
       61 taataaaata aaggagattc tgcattactt atagctctgt tctatgcgag ctcctaaatt
      121 tattatattg aattatatct atcagaacaa gattgattaa gttaaatgat aatatttgaa
      181 gatattgatt tagaacttca cacatgtata ttgctcttgc attaatcagg aattcaaaat
      241 ggaacttgag caattaatac aaagcgaaga gaataaagat aaagaacagc ttaatctaac
      301 tctacaaatc gcagaaaaag aatggaacaa aattagagaa tcccttagtc tttgcggaga
      361 tataggtgag ttttccaaag aagactttat ggttggaata attgaagagg acgtaattat
      421 aagacttccc aaattaagcc caacaaaatc agtatctgtt tacgcaccaa ctttttaccc
      481 catgtacttt attaaaaaca taaaggctat gagtgaaaat ttctctaaaa agggttacag
      541 gtcaactcaa gccctataca gctttattga gcttgcagcc aaggcagctg aaaggcttgg
      601 ccttagagga acattgagca tggcgtttgg tgttggatat gctaatgtac gtaccgggtg
      661 gatagctgaa aagggactac agaaagaaag agagattttt gaacaaatgt tcttttcagg
      721 aaagtttaaa aattatgact gggaaggcga ctggacgtct gtaaaaaaag ttttaaaaga
      781 aatatttgat aagtttaact cctggcagaa tgatgaagct ctttataaga gagaatccga
      841 tcctaaaaaa atagtaaagc ctatgcttgt ttaactaaaa agcatcctca tgttcttcgt
      901 ccagattttc gaagcttgga attccctgac tctctaaaaa tgcaagttta atacttcccg
      961 ttggtccgtt tctctgtttt ccaataatta actcagcagt accgtctttt tcatcctttt
     1021 tattcttgta cgcatcagcc ctgtgaatga acatcacaag atccgcatcc tgctccagcg
     1081 ctccactttc acgaaggtca gataactgag gacgcttgtc tgttctcgat tcaggctgtc
     1141 tactaagctg cgatatcgca ataaccggaa cggacagctc ttttgcaaga ctcttgagtg
     1201 aaccggaaat ttcagcaatc tctctttctc tcgtctcagt cctccggctt cctctcataa
     1261 gctgcaggta gtcaactatg ataaggtcca accccttatc ctttgaaagt cttcttgctt
     1321 tagccctaag ctccaaagaa cttatagccg gagtatcatc aatatatatt ccagccttac
     1381 tataaacatc agccgccttt acaagttttt ccagatcttt tgttccgata tatccgcttc
     1441 ttatgctcga ataactaacc tttgcctgga tggataacat cctaagcatg agctgttctt
     1501 ttgtcatttc cagtgagtaa aacgcaatat ttttcttgtt catgattgca gagtggcttg
     1561 ccagatttag acaaaatgag gttttgccaa gcccgggcct tgctgcaata attataagat
     1621 cagagggctg cagccccgag gtcatatgat ccagtctttc aaatccagtt ggaacaccag
     1681 tgagatgctc ttttctctta cttagcgctt cgatcatttc caatgcaccg gaggctagca
     1741 tgtttgtatt gtagaaactg ggtttaactc tgttctgacc aacctctaaa attgaatgct
     1801 cggccctgtc tacaagttcg tctacatcaa ccccgtcctc atggctttct tttgcaatat
     1861 ctgatgccgt tacaactagg tttcttaaaa tagacttttc tttaacaatc tgagcgtagt
     1921 atccaatgtt tgcagttgtg ggaacaattt cggtaagata tgtaaggtag ttacttcctc
     1981 ccacatcctc taacaactgc ccctttgcct ttaaaaattc aaacacagtg agaaggtcga
     2041 tgggttttga gtctctgtcc aggtctatca ttgcagagat aatttttttg tgtgagtcct
     2101 tataaaaatc ctctgttgtt aaaatttcca gaacctggtt tataaggctt ccttcaatta
     2161 ttagtgcacc aagtacagcc tgttcggctt ctaagttttg tggtaaagga cggctaagtt
     2221 ccatgtctat aacttaataa ctgatttaaa tttaacccct tgcagtgtta cgtaaaaata
     2281 tatattaaat ggtgaaaatt tgaaatagaa aattatggaa ttaggacaat acccaaatcc
     2341 ataacatttg tacctgttga tcctgtaata ataagatcac caaggtgatc aaaaaaatgg
     2401 tatgagtcat tatttgttat atgttccctt gcgcttacat ccatttttct tgctctggct
     2461 cttgtattac catcgcatat tgcccctgca gcatctgtag gaccatcaat accatcggtg
     2521 ccgcaaaaaa gacctattat attgtgtccg agtatttcca tagaaaaaga aagtgcggtc
     2581 tcggtatttc ttcccccctt accagagcct tctacagtta cagtagtttc accaccaaaa
     2641 ataagacatg caggtttttt tataggaaga tcgtattttt ctatttcaaa tgctatggct
     2701 gatatcgctt ttgcaacttc ccttgcctct ccgttcatct gtgaggataa gagcagtgaa
     2761 ttatatccaa gcttctttgc ctgttttttt gcagaaacaa gtgatttaaa gttgcttcca
     2821 acaactactg tttgaacctt ttgctgatct atatcctttg ccttaagtgt atcggcaatt
     2881 tctcccatag cacccctttc taaatgaagc actacctgag gaggaatacg gtgttcgagt
     2941 cttagagctt ctatcacctt ccagcactct ttaaacgttg tttcgtcagg tactgtaggc
     3001 cccgaagcaa tcccgtctaa tttgtcacct actacatctg ataatattag ggttgcaact
     3061 tgagaaggaa gagccttttt aagaagtcca ccgcctttta tttgagatat atgttttctt
     3121 atcgtgttta gtccataagt atctactccc gattttaaca acatctccgt tacgtttctt
     3181 ttttcatgca gagacagtcc ctcggcagga agtgcaagca tagagcttcc cccaccggat
     3241 attaaaaaaa tcacgaggtc atctttagtg ctgtgttcaa gcaactcgac tgcttttttt
     3301 gcagccttca tgcttttttc atcaggaata ggatggctgg attttaagta ctttagcttt
     3361 ttataagccg ggggtgaatc gtgatttgat accacaatac cgtcagttat ataggttcca
     3421 agagaatgct caacagcaag agccatctct gtagacgcct ttccaaaagc tattacatat
     3481 atgttcttaa aattatccag atcatatttt ttggtatcga tcttgagttt actattctta
     3541 atacttagtt tatccgaaac acacttatag ggatttgccg cctgcagggc ttcatgaaag
     3601 atatctatgc actgttttct atattttttg aagtccataa ttttacccac acaggcagta
     3661 taattgtatt tacaaactag ctcttttgta agtcttcaat gattgaatca actttacttt
     3721 ttgtaagatt ttcgtgaagc tgttcatcta ccagcatcac aggagctgtt ccgcatgcag
     3781 caaggcactc tgcttccata aaagttatct tgccatcggc agtagtttcc cctgtcttaa
     3841 cacatagttt atccccaata tagtgctcca cttgttctgc acccctaaga gcacaagaaa
     3901 gagtcctgca aagccagata acatgttttc cctttggttc attaaaatac atagtgtaga
     3961 aagtaaccac attctgaacg caagatggag atagttctag aatcgccgca atctcttcca
     4021 tggattcatt tgaaatgtgt ccaagctcat cctgtacagt gtgtagaaca ggaataagag
     4081 cagactgctt gttttcagca ctatttacat tatcagtaat tttagctttt agttctgtag
     4141 aaaatggcat ttgtaacacc ttattaaaat tctggattta aattacacta ttaataaaaa
     4201 tcttcaaaca tctgccatac taaaaattaa gattgtgtaa tgttgataga taaaaaaagg
     4261 cgggctactt aaataaatag taacccgccc tgtttgattt taaataaatt atcttacttg
     4321 tttaacaagt gttatatctg tctcatagag actacatcat gccgcccatt gct
//
LOCUS       DBNGSV010000295        13626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_537443, whole genome shotgun sequence.
ACCESSION   DBNGSV010000295 DBNGSV010000000
VERSION     DBNGSV010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_537443"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /gene="def"
                     /locus_tag="AC159U_10750"
     CDS             <1..363
                     /gene="def"
                     /locus_tag="AC159U_10750"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3337568.1"
                     /translation="AAPQVGHNIRVIVVDVEHNKEGDKNLIELINPEIIESYGSIKGE
                     EACLSIPGFTAYVKRKAEVAVKGLNRSGKIIEIEAADLLSRVLQHEIDHLDGILFIDR
                     LSRLKRELIKSKIKKAYS"
     gene            complement(372..566)
                     /gene="rpmB"
                     /locus_tag="AC159U_10755"
     CDS             complement(372..566)
                     /gene="rpmB"
                     /locus_tag="AC159U_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3337569.1"
                     /translation="MAQVCDICGKKPMFGNNVSHANNKTRRRWNINLRSVKAALDSGG
                     TKKMKVCTRCIRSGKVKKAA"
     gene            695..1825
                     /locus_tag="AC159U_10760"
     CDS             695..1825
                     /locus_tag="AC159U_10760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3337570.1"
                     /translation="MISKLANSISPFYVMEVLERAQKLEKQGTDIIHLEIGEPDHNTD
                     DKICKEAITAINAGDTKYTHSLGISDLREQLCLYYKKKYGVSISAENIIVTNGSSPAL
                     FLAFCSVLNPGDEIILTDPHYACYPQIIRIAGGVPKFVRIYEKEDFQIDIKRLKKKIS
                     KKTRAILINSPSNPTGMLVNSDIMQQLSKLGIPIISDEIYHGLVYEGREQSILEFTKD
                     AIVVSGFSKLYSMTGWRLGYLIVPDDYVRPIQKLQQNLFISANSFVQRAGIEALKMSD
                     SQINSIVQKYKKRRDAMLTGLKSLGFKIQRVPDGAFYFFVNTKKISTNSYDLAFDLLE
                     NAHVALTPGIDFGKGGQGYMRFSYANSITNINEGISRLKKYL"
     gene            1889..3040
                     /gene="pgk"
                     /locus_tag="AC159U_10765"
     CDS             1889..3040
                     /gene="pgk"
                     /locus_tag="AC159U_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3337571.1"
                     /translation="MRTDYDFASDTDAAEIDFKINSSLYTIQYAIENGAKLIIASHRT
                     VKEHKYDPQYSLGFVGDILSERLNTNIYFPDDCIGDAVKKIKQDMNQGDVMLLENLMF
                     YKDEIKNESAFSRKLSENLDIYVSEAFGLVNKSYSSNSSMLEYVNEKCAGFSFSDELM
                     LLGEIKTCKQKPFVVFYNGDDVYAKLEFLEDMKENIDVLMLGGVFANTLLKSIGYEIS
                     DGYYDKESVYRINKLYKSLIARNKKVVLPEDFMINIKHEKSEGFYIDELSKLKENFQI
                     TGVGNKTQQSFIKYINEASIILWDGGNCFTDMYRKFKKDKGDKDCKTDPITAELINNN
                     EKLKIICDYNTSRYFNNDNENLQISYNIDCAYSYLKGENLPGLQALENN"
     gene            3059..3820
                     /gene="tpiA"
                     /locus_tag="AC159U_10770"
     CDS             3059..3820
                     /gene="tpiA"
                     /locus_tag="AC159U_10770"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3337572.1"
                     /translation="MGKKTVIANWKMNLLQDEARELVNHIVSNVSQSALKKVDVILAP
                     PYTCLQPVGQILEGSGIELCAQNVFWEDAGAYTGEISADMLMELGCKWVIIGHSERRN
                     ILGETNSMINDKIIFSLKKGLKVIFCIGERIEEKTRGRTLSVIKKQLEIGLNGVDAGH
                     LDDIIFAYEPVWAIGSGITAEPEQADIVHLTIKEFVSDRFDCILPKIQVLYGGSVKSG
                     NINSLISQPNIDGVLVGGASLDKDSFIEIIKSSAS"
     gene            3837..4298
                     /gene="secG"
                     /locus_tag="AC159U_10775"
     CDS             3837..4298
                     /gene="secG"
                     /locus_tag="AC159U_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3337573.1"
                     /translation="MEIVETLITFLHIIVCLIIIVAVLLQPGKSGDLGSMFGGGTSES
                     IFGSSGAVPFLAKLTRVLGVVFFATSLFLGYFAIKDNSSSVLKGVGASTSGFEETFGT
                     DSDLIGSLDDVQQEVTETVETQPADKPVENSDDGMKLKEIPEQSKTEKQTE"
     gene            4376..4642
                     /gene="fdxA"
                     /locus_tag="AC159U_10780"
     CDS             4376..4642
                     /gene="fdxA"
                     /locus_tag="AC159U_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005505213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3337574.1"
                     /translation="MAYIIAEPCIDVKDKACVEACPVDCIYEADDQLVIHPEECIDCG
                     ACVDPCPVDAIYHEDELPDKWQKFIDLNKDFFEGKSDLEPARKD"
     gene            4722..5369
                     /gene="nth"
                     /locus_tag="AC159U_10785"
     CDS             4722..5369
                     /gene="nth"
                     /locus_tag="AC159U_10785"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3337575.1"
                     /translation="METTDIKEKKKRLGEILKIFKKEYPDPKCHLDFKNPFELLIGCI
                     LSAQCTDKRVNQITPNLFAKYKNPAGFSVAKQKELEKDIHSAGFFRNKAKSIIGCAKG
                     LQKDFGGKVPKTMDELTTLCGVGRKTANVILANAYEIPGIIVDTHINRLSTRLGLTSH
                     KDPTKIEFDLMEIVPKKKWAYFSNALGDHGRTVCSSQKPKCGICKLNHLCPAARL"
     gene            5377..6288
                     /gene="mnmL"
                     /locus_tag="AC159U_10790"
                     /gene_synonym="ytqA"
     CDS             5377..6288
                     /gene="mnmL"
                     /locus_tag="AC159U_10790"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR3337576.1"
                     /translation="MQRYNSYSRYLLETFGCKVNKISLDLGFTCPNRDGSKAIGGCIY
                     CNNDTFVPPYARSRYTLDQQMTNGMKYLRARFKAKKFIVYFQAYTNTYSTVKELEDLY
                     RQALGYEDVIGLAIGTRSDCIDEEKLDMIERLSEEVYVSLEYGIESIYDKTLDYMNRG
                     HDYESVLKAIELSKGRGLHLGVHIIVGFPTETEEQMLAMANEVSGLGIDVLKVHNLHV
                     VKNTPLARMYAENPFHLFSYEEYLSFITRFIERVSPDVVIERLFTDTPHQLLIAPIWP
                     KNHLEVLRGIDAELLKQDTYQGKFYSS"
     gene            6305..7399
                     /gene="dprA"
                     /locus_tag="AC159U_10795"
     CDS             6305..7399
                     /gene="dprA"
                     /locus_tag="AC159U_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3337577.1"
                     /translation="MDEYLHWIALKNVKGIGEVLYKRLLSHFSSPKEVFEAGPKEFLQ
                     IEGISPDTVKLIKSFDDWESVEKELQKIKKLNLRLMVMSDPAYPDNLKNIYNPPPYFY
                     MRGNIVEQDHRSIGIVGSRLSDTYGKKVTEQIAGELAVNGYTVVSGFARGIDTIAHKA
                     CLKAGGRTIAVLGSGIDVVYPSENATLYKEIESNGAVISEFAIGTGPVAVNFPRRNRI
                     ISGLSVGVLVVQAAQKSGSLITAEFALEQNREVFAIPGNIGSNLSRGTNRLIKKGAKL
                     VNTVDDILEEIEGITGVRKELPRRNEVDIESLENSEKIIYKVLKEGQHHVDQIIKNTG
                     LDSSRVLTDLLNLEIKGIVSQLPGKYFELV"
     gene            7518..8201
                     /locus_tag="AC159U_10800"
     CDS             7518..8201
                     /locus_tag="AC159U_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3337578.1"
                     /translation="MNKTASAEESTDSIQILLGCSSSILGEGIKKLIEEDADLKVVQK
                     ITSLLDVFEHYKNTSVDVFVIEYELPGFEVSKIKNLINKNSNIKILVLLNKEYEDEVL
                     VKTIRAGASGYVLTGSDSSHLRKAVKGVCQNELWVERRILSRVVKDVINENKADTEKV
                     DSTIVELTEAESRIVKLVLQGLSNRAIADHLYLSEKTVKFHLYKTFKKLKVKNRSELI
                     LYCFKNKLI"
     gene            8439..10550
                     /gene="topA"
                     /locus_tag="AC159U_10805"
     CDS             8439..10550
                     /gene="topA"
                     /locus_tag="AC159U_10805"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3337579.1"
                     /translation="MPKSLVIVESPAKAKTIKNYLGKDFTVEASSGHLIDLPKSKLGV
                     DIENDFQPQYEVIKNKKKYLTKLQKAAQKADSVYLASDPDREGEAIAWHIADQLGIQD
                     KSYRILIHEITEKAIKESINSPTTLSMDRFDAQQARRVLDRLVGYQVSPLLWKKVRRG
                     LSAGRVQSVALRIVVEREREIEKFVTKEYWSIEGDFIAEQNGKSAFSANLVRFDGDKI
                     EIGTEDQAQSIANQIKNNDYKVSSIEKKERTRKAQPPFITSTLQQEASRKLRYPVKKT
                     MAIAQKLYEGIELGADGPMGLITYMRTDSVRISDQALAESRSLIKDKYGDKFLPEKPN
                     QYKVKKSAQDAHEAIRPTSFEYSPEKVKAHLADDEFELYKLIWQRFLASQMTPIVYDQ
                     TSIEISSDKATFRTTGSVIKFEGFSKVYTEGKEEEEEQSDDEDKKLPDIKKDQDVNLK
                     DLKMEQHFTQPPPRFSESSLVKELEEKGIGRPSTYSSIISTIQDREYVKREQRKLKPT
                     VLGKSVNDMLIDGFPEIMDIKFTADMEDRLDKVEDGDINWVELMKNFYKGFSERLSVA
                     DEKMRSLRRDGVPAGIDCNECGVPMIIRWGKRGEFLSCSKYPECKNAKPFHYDEDGNV
                     KVVEKVEPEVSDIECDKCGKPMVIRQSRYGKFYGCSGYPECKNIKRMQKEKASEKKEA
                     QDDDAEESKSKNKRKVITVST"
     gene            10649..13120
                     /gene="recG"
                     /locus_tag="AC159U_10810"
     CDS             10649..13120
                     /gene="recG"
                     /locus_tag="AC159U_10810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3337580.1"
                     /translation="MNKISKILSDIERPLLFAAKNDFKNIGKLTGTAQYIAQMRIKAI
                     SLKVDNKTEKVLSEIESSFNQFESKSKGEQKEIVSSSLKLINDLKENIVSKPDPAVTK
                     KEQPAKVQSNIKSDSPVQYIKGVGPKISQMLERKGISSVNDILYYFPRRYEDRRNLNT
                     ISDISQDEPTTVIGKIELSGVVKIRTRSIYKIVISDGTGFANLIWFQFHKSYLSKLYK
                     KGLYAVVSGVFTYDKYSGAFQLIHPKPEDIEVVETKEELEQDSTNFNRIVPVYHLTEG
                     LKQKRIRNIVRNVLDQDFDISDPIPSSVRDKHSLLGLKDAMSRVHFPLAEDTVVDLEN
                     GEHIQKSRPHFTVAYFELFMLQLGLALKRKKLDEVDGISFKNDNNMPRELLGNLKFAL
                     TGAQKRVIKEIYKDLDSEKQMNRLLQGDVGSGKTIVALLSILKVIESGYQAVFMAPTE
                     ILSEQHYKNLTSYLKDFDLNIVLLKSAITRAARTEVLESIKDGSANIIIGTHAVFQKD
                     VDYKKLGLVVIDEQHRFGVAQRSDLIKKGLTPDVLIMTATPIPRTLSMAYFGDLDISV
                     IDEMPKDRKKIATKVYYNDEKNRQRAYDVVAAELKAGRQAYIVAPMIEESENTDFKHL
                     KYVNDLADELSEGYLSGFKLEALHGQMKSEQKHEIMTSFLGKQIDVLVATSVIEVGID
                     VPNASVIVIENAERFGLSQLHQLRGRVGRGEFQSKCLLVSSYKRSELAGKRLSVLEQT
                     NDGFKIAETDLLIRGPGEFIGTRQSGIPELKFANIIRDTAILRLAKMDAFELIDNCES
                     EEQLKNYTKMLQEKWGESLEFASVS"
     gene            complement(13123..13353)
                     /locus_tag="AC159U_10815"
     CDS             complement(13123..13353)
                     /locus_tag="AC159U_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337581.1"
                     /translation="MDNKFEYIATQTDDGFVVNLKNAVNDTIEIKNEDIEIFAKTLSD
                     KLVTDRDIILTEKEEILFNIWQMLLVPENIVH"
BASE COUNT         4682 a         2303 c         2923 g         3718 t
ORIGIN      
        1 gctgcacctc aggtaggtca caatataaga gtaattgtgg tagatgtgga gcataataag
       61 gagggtgaca aaaatctcat agagcttata aatcctgaaa tcatagaatc atacggaagt
      121 ataaaaggtg aagaagcgtg tctcagtatc ccggggttta cggcttatgt taagagaaaa
      181 gcagaggttg cagtaaaggg tttaaatagg agcggaaaaa taatagaaat tgaggcagca
      241 gatcttcttt cacgcgtact acaacatgaa attgaccacc ttgatggaat attgtttata
      301 gacaggctga gtagacttaa aagagagcta attaagagta aaataaaaaa agcttattcc
      361 taaaaatgaa tttaggcagc ttttttcact tttcccgaac ggatgcatct ggtacaaact
      421 ttcatctttt ttgtaccacc gctatcaagt gcagctttta cgcttcttag gtttatattc
      481 cagcgccttc ttgttttgtt gttagcatga ctaacattgt tgccaaacat cggctttttt
      541 ccacatatat cacaaacttg agccattttt tcttatcctt cctaaaatta aaaaactcaa
      601 tatacccttt gcttttatct atttcaatat gtttattatt ttattaatta aatttcctag
      661 acaaagacaa tgtttttaat agaatacaaa ttctatgatc tctaaactag caaattctat
      721 ttcacccttt tatgtgatgg aagtgctcga acgcgcacaa aagcttgaaa aacaaggcac
      781 cgacataatt cacttagaaa taggcgaacc agatcataat actgatgata aaatatgcaa
      841 agaagcaatc acggcgatca atgcaggaga tactaaatat actcacagtc ttgggatatc
      901 agatttaaga gagcagcttt gcttgtatta taagaaaaaa tacggtgtta gtatttctgc
      961 agaaaatatc atagtcacca acggaagctc tcccgcactg tttttggctt tttgctcggt
     1021 tctaaacccg ggagatgaaa taatccttac agaccctcat tacgcctgct atcctcagat
     1081 tattagaatt gcaggaggag taccaaaatt tgtaaggata tatgaaaaag aagattttca
     1141 gattgatata aaaaggctaa aaaagaagat ttcaaaaaaa accagagcaa tattaataaa
     1201 ttcaccatca aatcccaccg gaatgcttgt aaacagtgat attatgcagc agctttcaaa
     1261 gttaggaatt cccatcatat ccgatgagat atatcacggt cttgtatatg aaggcaggga
     1321 gcagtcaatc cttgagttta caaaagatgc tatcgttgta agcgggtttt caaagttata
     1381 ctccatgacc ggatggaggc ttggttatct cattgttcct gatgactacg taaggcccat
     1441 acaaaaactg cagcaaaatc tttttatctc ggcaaactca tttgtacagc gtgcgggtat
     1501 tgaagctcta aagatgagtg attctcaaat aaacagtatt gtacagaagt ataaaaaaag
     1561 aagggatgca atgttaactg gtctcaaaag tcttggattt aaaatacaaa gagtacccga
     1621 tggggcattt tatttttttg taaatacgaa aaaaataagt accaactcct atgatctggc
     1681 atttgatctt ttagaaaatg cccatgtggc tttaactccg gggatcgatt ttggaaaagg
     1741 tgggcaggga tatatgagat tttcatatgc aaactctata accaatataa atgaaggaat
     1801 atccaggctt aaaaaatatt tatagtattg tacgttgatg aatattaaat ctattgaaca
     1861 actcgatatt gaaaactagc ttttattcat aaggacagat tatgactttg cctctgacac
     1921 tgatgctgct gaaatagact tcaagataaa ctcttcactt tatacaatac agtacgccat
     1981 agaaaacgga gcaaagctta taattgcttc tcacaggaca gtaaaagaac ataaatatga
     2041 cccgcaatat tccctggggt ttgtgggaga tatcctcagc gaaagactaa acaccaatat
     2101 ctattttcct gatgactgta ttggggatgc agtaaaaaag ataaagcagg acatgaacca
     2161 gggtgatgta atgctcctgg aaaatcttat gttctataag gatgagataa aaaatgaatc
     2221 tgctttttcg cgaaagcttt ctgaaaattt agatatatat gtaagtgagg catttgggtt
     2281 ggttaataag tcatatagtt caaattcgtc aatgctcgaa tatgtaaatg aaaaatgcgc
     2341 agggttttca ttctctgatg aattaatgct tctgggtgag attaaaacat gtaagcaaaa
     2401 gccttttgtt gttttttaca atggagacga tgtatatgca aaactagagt tcttagaaga
     2461 tatgaaagaa aatattgatg tgcttatgct gggtggggta tttgcaaata cgcttctaaa
     2521 atcaattggt tatgaaatat cagatgggta ttacgataag gagtctgtat acaggataaa
     2581 taagctttat aagtcactaa ttgcaaggaa caaaaaagtg gtactgcctg aggattttat
     2641 gattaatatt aaacacgaaa aatcagaggg cttttatata gatgagcttt caaaattaaa
     2701 agaaaatttc cagataaccg gcgtcggaaa taaaacacag caatcattta taaaatatat
     2761 aaatgaggca agtattattc tttgggatgg tggtaattgt tttactgata tgtatagaaa
     2821 atttaaaaaa gataaagggg ataaagattg taagactgat cccataacag cagagttaat
     2881 caataataat gagaaactaa aaataatttg tgactataat acttccagat attttaataa
     2941 tgataatgaa aatttgcaga tttcatataa tattgattgt gcatattctt atttaaaggg
     3001 agaaaatctg ccagggctgc aagctttaga aaataactaa aagcactgag taacaaaaat
     3061 gggtaaaaaa actgtaattg caaattggaa gatgaaccta ctgcaggatg aggctaggga
     3121 gcttgtaaat catatcgtat caaatgtctc acaatcagcc ctcaaaaaag tagatgtgat
     3181 actggctcct ccatatactt gtctgcagcc tgttggtcaa attctagaag gctctggtat
     3241 agagctctgc gctcaaaatg tattctggga agatgcgggt gcatacaccg gagaaatttc
     3301 tgctgatatg ctcatggaac ttggctgcaa atgggtgatt attggtcatt cggagagaag
     3361 gaatattctg ggtgagacca actccatgat aaatgacaaa attattttct cactcaaaaa
     3421 agggcttaaa gtaatattct gtattggtga aagaattgag gaaaaaacaa gaggccggac
     3481 tcttagtgtt ataaaaaagc agttggagat agggctaaat ggtgttgatg cggggcatct
     3541 ggatgatatt atttttgcct atgagcctgt atgggcaatc ggatctggta ttacagccga
     3601 acctgagcag gcagacatag tacaccttac cataaaagag tttgtaagtg acagatttga
     3661 ttgtatatta cctaaaattc aagtactgta tggcggaagc gtaaaatcag gtaatataaa
     3721 tagtttaatc tcccaaccca acatagacgg agtactggtt gggggagcaa gtttggataa
     3781 agattcattt atagagataa taaaatcatc agcaagttag acaggagtat aaagaagtgg
     3841 aaatagttga aacactaatc acatttttac atattatagt ttgtttaata attattgttg
     3901 ccgtgctgct gcaacccggc aaaagtggag acctcggctc tatgtttgga ggcggtacct
     3961 ctgagtcaat cttcggatca agcggtgcag taccttttct tgcaaaactt acccgggtct
     4021 taggcgtagt cttttttgcg acttcacttt ttcttggata ttttgcgatt aaagataatt
     4081 catcatcggt tttaaaaggc gttggcgcat ctacctcagg atttgaagaa acgtttggta
     4141 cggattctga tcttattggt tcgcttgacg atgtgcagca agaagtaaca gaaactgtag
     4201 aaacacaacc tgctgacaag cctgtagaaa atagtgatga tggaatgaaa ctaaaagaaa
     4261 tacctgaaca gagcaagact gaaaaacaaa ccgaataggt ttatttttac tctcttgaat
     4321 tcaaaccctt gtgcagtata ttagttattc tacttatttc taggaggttt ttttaatggc
     4381 ttacataata gctgaaccat gtatcgacgt taaagataag gcctgtgtag aggcatgtcc
     4441 tgtagattgt atctatgaag ctgatgatca gcttgtgata caccctgaag agtgtattga
     4501 ttgcggcgct tgtgttgatc cctgtcctgt ggatgctatt taccatgaag atgaactccc
     4561 tgataaatgg cagaagttca tagacttaaa taaagatttc tttgaaggca aatcagacct
     4621 ggagcctgca agaaaagatt gatcttgtta aaacacgaaa acaaatgatt taacacaagg
     4681 gatatgctaa agcatatccc tttttttttg ctattttggc tatggaaacc actgacatta
     4741 aagagaagaa aaaacggctt ggtgaaatac ttaaaatttt caaaaaagaa tatccagacc
     4801 caaagtgtca tctggacttt aaaaatccat ttgagctgct tataggatgt atattatcgg
     4861 cccagtgtac tgataaaagg gtaaaccaga taacccctaa cttgtttgct aaatataaaa
     4921 atccagccgg tttttctgtg gctaaacaaa aagaacttga aaaagatatc cattctgctg
     4981 gatttttcag aaataaagcc aaatccataa ttggctgtgc taaaggacta cagaaggatt
     5041 tcggtggcaa agtgcccaaa acaatggatg agcttactac cctttgcggg gttggaagaa
     5101 aaactgcaaa tgtaattttg gcaaacgcat atgaaatccc cggtataata gtggatactc
     5161 atataaatag gctaagcacg agacttgggc ttacaagcca caaggatccg accaaaatag
     5221 agtttgatct aatggaaata gtaccaaaga aaaaatgggc ttatttttca aatgcccttg
     5281 gagaccacgg aaggaccgta tgcagctcac aaaaaccaaa gtgcggaata tgcaaattaa
     5341 atcatctgtg cccggctgcc aggctttgaa aatatcatgc agagatataa ctcatacagc
     5401 agatatttat tagaaacttt tggatgcaag gtaaacaaga tctcacttga tctgggtttt
     5461 acctgtccca atagagacgg ctccaaggca atcggaggct gtatatactg taataacgat
     5521 acttttgttc caccctatgc acggtcgaga tatacgcttg atcagcagat gacaaacggg
     5581 atgaagtatc tgagggcacg gtttaaggcc aagaagttta ttgtctactt tcaggcctat
     5641 acaaacacat actccacggt taaggaactg gaagatttat acaggcaggc tctgggttat
     5701 gaagatgtaa ttgggcttgc gattggaaca cgtagtgact gtattgatga agaaaaactg
     5761 gatatgattg agaggctttc agaggaagtg tatgtctcgc ttgagtatgg tattgaatct
     5821 atttatgaca aaacgctcga ttatatgaac agaggacatg actatgagtc tgtactgaaa
     5881 gccatagagc tttcaaaggg aagggggctt catttgggag tacatataat tgttggtttt
     5941 cctacagaga ctgaagagca gatgcttgcc atggcaaacg aggtctcagg tcttggaata
     6001 gacgtactaa aagtacacaa ccttcatgtc gtaaaaaaca ccccgcttgc cagaatgtat
     6061 gctgaaaacc cgtttcatct gttcagctac gaagagtatt tgtcttttat cacaaggttt
     6121 atagaaaggg tatctcctga cgttgttatc gaaagattgt ttaccgatac gcctcatcaa
     6181 ttactcatag ctcctatatg gccaaaaaac cacttggagg tcctaagagg tatagatgca
     6241 gagctgctaa agcaagacac ctatcagggg aaattttata gttcttaatt aattgcaaat
     6301 tgctatggat gaatatctgc actggatagc tcttaaaaat gtaaaaggaa tcggagaggt
     6361 tttatataaa agactccttt ctcatttttc ttctccaaaa gaagtattcg aagccggtcc
     6421 aaaagagttt ttacagattg agggtatcag tcccgatacg gttaaactca ttaagtcgtt
     6481 tgatgactgg gaatctgtag aaaaagagct tcaaaaaata aaaaaactaa atctgcggct
     6541 aatggtaatg tcagaccctg cctatcccga taatcttaag aacatttata accctccgcc
     6601 ttatttttat atgagaggaa atatagtgga gcaggatcac aggtcaatag gaatcgtagg
     6661 gagcagactc tcggatacat atggtaaaaa agtgaccgag cagattgcgg gtgagcttgc
     6721 agtgaatggc tatacggttg taagcggttt tgcccggggt atagatacaa tagctcacaa
     6781 ggcatgccta aaggcaggag gcagaactat tgcagtactt ggctcaggca ttgatgtggt
     6841 ctatccctca gaaaacgcta cattatataa agaaattgag agtaacggag ctgtgatttc
     6901 tgagtttgcc attggaacgg ggcctgttgc agtaaacttt ccaagaagaa acaggattat
     6961 tagcggcctc tcggtgggag tgcttgttgt tcaggcagca caaaaaagcg gctcccttat
     7021 tacagcggaa tttgcgcttg agcaaaacag agaagtattc gcaattcccg gaaacatagg
     7081 aagcaatctt agcaggggaa caaacaggct tattaaaaaa ggagcaaaac ttgtaaacac
     7141 agttgatgat atcctggaag agatagaggg tattacgggg gtaagaaaag agcttccacg
     7201 aagaaatgaa gtggacattg agagtctgga aaattcggaa aaaattattt ataaggttct
     7261 taaggaaggc cagcatcacg ttgatcaaat aataaaaaac accgggcttg acagctcccg
     7321 ggttctcact gatctattaa atctggagat aaaaggcata gtctctcagc ttccgggcaa
     7381 gtattttgag cttgtttgat aaatatttat agaataatca gataaacggt tataattttg
     7441 ctcaaattgg tttataataa atataatttg taataaaatg ctggttagta gtaaaaatac
     7501 atgatgaagg tgtttaaatg aacaaaaccg caagcgctga ggaaagtacg gacagcattc
     7561 agattcttct tggatgcagt tcatcaatac tgggtgaggg aataaaaaaa cttatagaag
     7621 aagacgctga tttaaaagtg gttcagaaaa ttacaagcct tctggatgtt tttgaacact
     7681 ataaaaacac ttcggtagat gtgtttgtta tagagtatga actgcctggg tttgaagtat
     7741 ctaaaataaa aaatttaatt aataaaaaca gcaatattaa aatacttgtg cttcttaata
     7801 aagaatatga agacgaagta ctagtaaaaa caattagagc tggtgcaagc ggttatgtgc
     7861 tgactggttc tgattcaagt cacttgagaa aagcggtaaa gggagtctgc cagaatgagc
     7921 tctgggtaga aagaagaatc ctctcaagag tggtaaaaga cgtaataaat gaaaataagg
     7981 cagatactga aaaagtagac agcacaattg tagagcttac agaggctgag tccagaatag
     8041 taaaactggt tttacagggg cttagcaaca gggcaattgc cgatcatctt tatctgagcg
     8101 aaaaaaccgt aaaatttcac ctctataaga catttaagaa attaaaggta aaaaacagat
     8161 cagagcttat tctttactgt tttaaaaata agcttatttg ataccacttc taaaagcttt
     8221 tatatcttgt ccgggttata gataatataa aaaaccctaa cagttttata gatttttcct
     8281 cattgctacc aagcgtatac ttgctcttgt tttatacctg ttttaaaaat aatattttga
     8341 tttaaataat ctttgtttac cttatttaaa cattattaat ttgtaatttt aataaatatg
     8401 tggttattat catttgaaat tagtgtcaga agataaatat gcctaaatca cttgtaatag
     8461 tagaatcacc ggcaaaggcc aagaccataa agaactatct tggaaaggat tttaccgtag
     8521 aagcttcctc cggtcatctg attgatcttc caaagagtaa gcttggagtc gatattgaaa
     8581 atgacttcca gccccagtat gaagtaatta agaataaaaa gaaatattta accaaactgc
     8641 aaaaagccgc tcaaaaagcc gacagcgtgt atcttgcctc agacccagac agagagggtg
     8701 aagcaattgc gtggcatatt gccgatcagc ttggaattca ggataaatca tatcgtattc
     8761 tgattcacga aataacagaa aaagctatca aggaatcaat taactcccct accactctta
     8821 gcatggacag gtttgatgcc cagcaggcaa gaagggtgct cgacaggctt gtaggatatc
     8881 aggtaagccc gctgctgtgg aaaaaagtga gaaggggcct tagtgcaggc agggtgcaga
     8941 gtgtggcgct taggatagtg gttgaaagag aaagagagat tgaaaagttt gtaacaaagg
     9001 aatactggag tatagaagga gactttattg ctgagcaaaa tggaaaatct gcattttcag
     9061 caaacctggt ccggttcgat ggcgacaaaa tcgagatagg aacagaagat caggcacaat
     9121 caattgcaaa ccagattaaa aacaacgact ataaagtatc atccatagaa aaaaaagagc
     9181 gcacaagaaa ggctcagccc ccatttatta caagcacgct tcagcaggag gcttcgagga
     9241 aactgaggta tccggttaaa aagacgatgg caatagcaca gaagctctac gaaggaattg
     9301 agcttggtgc agatggaccc atggggctga ttacttatat gagaaccgac tctgtgcgta
     9361 tctcggatca ggctcttgca gaatcaagaa gcctgataaa ggataaatac ggcgacaagt
     9421 ttctgccaga aaaaccaaac cagtataagg taaagaaatc tgctcaggat gcgcacgaag
     9481 ccataaggcc cacatcattt gaatacagtc ctgaaaaagt aaaggcccat cttgcagatg
     9541 atgagtttga gctctacaag cttatatggc agaggttctt agcttctcag atgactccta
     9601 ttgtttacga tcagacttca atagagattt catcggacaa ggcaacattc agaacaacag
     9661 gctctgtaat aaaatttgaa gggttttcca aggtatatac cgagggaaaa gaggaagagg
     9721 aagaacagag cgatgatgaa gataaaaaac ttcccgatat caaaaaagat caggacgtaa
     9781 acctaaaaga ccttaaaatg gagcagcact tcacacagcc ccctccaagg ttttctgaaa
     9841 gctcgctagt aaaagaactt gaagaaaagg gaataggaag accatctact tattccagta
     9901 ttatttcaac aatccaggac agagagtatg ttaagcgcga gcagagaaag ctaaaaccca
     9961 cggtgctcgg aaagtcagtg aatgatatgc tcatagacgg ttttcctgaa atcatggaca
    10021 ttaagtttac tgccgacatg gaagacaggc tcgataaggt agaagatggt gacatcaatt
    10081 gggttgagct tatgaagaac ttctacaagg gtttttcaga gcgcctctct gtagccgatg
    10141 aaaagatgag aagtctcaga agggacggag ttcccgctgg gattgactgt aacgaatgcg
    10201 gagtgcctat gattattaga tggggaaaaa gaggggaatt cctatcatgc tccaagtacc
    10261 ccgagtgtaa aaatgcaaaa ccgtttcatt atgatgaaga cggaaacgta aaggtagtag
    10321 aaaaagtcga gcctgaagta agtgatatag agtgcgataa atgcggaaag cccatggtca
    10381 taagacagag ccgctacggc aagttttatg gatgcagcgg ataccctgag tgtaaaaaca
    10441 taaagcggat gcaaaaagaa aaagcatcgg aaaaaaaaga agctcaggac gacgatgctg
    10501 aagaaagcaa gtccaagaac aaacgcaagg ttatcaccgt aagtacttaa gattatgtcc
    10561 atccggcagt tgcggatata attattctat aagattagca gtcttaccat cttatctggt
    10621 cttaagataa acatattaaa atattcacat gaataagatt tccaaaatct taagtgacat
    10681 agaaagacct ctactctttg ccgctaaaaa cgattttaaa aatataggca agctcactgg
    10741 tactgctcaa tacattgctc agatgaggat taaggctata agcctcaaag tggacaataa
    10801 aaccgaaaaa gtattgtcag aaattgaaag cagtttcaat cagtttgaat caaagagcaa
    10861 aggtgagcaa aaagaaatag tctcttcttc tttgaagctt ataaatgacc taaaagaaaa
    10921 tattgtcagt aaaccagatc cggcagtcac caagaaagaa caacctgcta aggttcagtc
    10981 caatatcaag agcgattctc ccgtgcagta tataaagggt gtaggtccaa aaatatccca
    11041 gatgcttgag cgaaagggga taagctcggt aaatgatatc ctctattact tcccaagaag
    11101 atatgaagac agaagaaacc tgaataccat ttcagatatt tctcaggacg aacccactac
    11161 ggtgatagga aagatagaac tatccggggt ggtaaaaata aggaccagga gtatctataa
    11221 aatcgtgatc tccgatggaa ccgggtttgc aaacctcata tggtttcagt ttcataaaag
    11281 ctatctgagt aaactgtata aaaagggtct ctatgccgta gtgagcgggg tgtttactta
    11341 tgataaatac tcgggtgcct tccagctcat tcatccaaaa cctgaagaca ttgaagtggt
    11401 cgaaaccaaa gaagagcttg agcaggacag taccaatttt aacaggatag tgccggtata
    11461 tcatcttaca gaaggcctca agcaaaagag aataagaaat atagtaagaa acgtcctgga
    11521 tcaggatttt gatatttcag accccattcc atccagtgtg agagataagc atagtctgct
    11581 gggccttaaa gacgccatga gccgggtgca cttcccactt gctgaagata ctgttgtgga
    11641 tctggaaaat ggggagcata tacaaaaatc caggccgcat tttaccgtgg cctattttga
    11701 actgttcatg cttcagctcg ggcttgcgct taaaagaaaa aaactggacg aggtagacgg
    11761 catttcattt aaaaatgata acaatatgcc aagagaactt ctcggtaacc tcaaatttgc
    11821 actcaccggc gctcaaaaaa gggtgattaa ggaaatatat aaagacctcg attcagaaaa
    11881 gcagatgaac aggctgctgc agggtgatgt agggagcggc aaaacaattg tagccctgtt
    11941 atctatactc aaagttattg aatcaggcta tcaggccgtg ttcatggctc caacagaaat
    12001 actttccgag cagcactata agaatcttac ttcttacctt aaggattttg atcttaatat
    12061 tgtcttgcta aagagtgcaa taacaagggc tgcaagaacc gaagtattgg aatccataaa
    12121 agatgggagc gcaaatataa taataggcac ccatgccgtg tttcaaaagg acgtggacta
    12181 taagaagcta ggacttgtag ttattgacga gcagcacagg tttggagtag cccagagatc
    12241 tgatctaata aaaaaggggc tcacaccgga tgtactcatc atgacagcca cgccgatccc
    12301 aaggactctt tcgatggcat attttggtga cctcgatata tcggtaattg atgagatgcc
    12361 aaaggacagg aaaaagatag caacaaaggt gtattacaat gatgaaaaga acaggcagag
    12421 agcatatgat gtagttgctg ccgagcttaa agcgggaagg caggcctata ttgtggctcc
    12481 gatgatcgag gagtctgaaa acaccgattt taagcacctt aaatatgtaa atgatctggc
    12541 agacgagttg agtgaggggt atctgagcgg atttaagcta gaagctttgc acgggcagat
    12601 gaagagcgaa cagaaacatg aaattatgac ttcatttctt ggtaagcaga tagatgtgct
    12661 tgtggcaacc tcagtaatag aagtaggaat agacgttcca aatgcaagcg tcatagtcat
    12721 tgaaaatgca gaaagattcg gcctttcaca gctgcatcag ctgcggggaa gggttggcag
    12781 gggagagttc cagtcaaaat gtctccttgt ctcaagctac aagaggtcag agcttgccgg
    12841 caaaaggctc tcagtgctgg aacagacaaa tgacgggttt aaaattgcag aaactgatct
    12901 cctcatcaga ggacccgggg agtttattgg cacaaggcag tcgggaatcc ctgagcttaa
    12961 gtttgccaat atcataagag atacagcaat tcttaggctt gccaaaatgg acgcatttga
    13021 gttaatagat aattgtgagt cagaagagca gcttaaaaac tataccaaaa tgttacagga
    13081 aaaatgggga gagagcctgg agtttgcctc tgtatcttag agttagtgga ctatattttc
    13141 aggaacaagg agcatctgcc agatattgaa cagaatttcc tctttttcgg tgagtataat
    13201 gtctctgtct gttacaagtt tatcacttag agtctttgca aaaatctcta tatcttcatt
    13261 ttttatctca atcgtgtcgt ttacagcatt ttttaaattt acaacaaagc cgtcgtctgt
    13321 ctgagtagcg atatattcga acttattgtc catatctaac ctcctacttt agtatttatg
    13381 ccttgtaata ctgtaaacta atcacttata aacttttgta aagagactct atgaaaagca
    13441 atttatgaat cagttttgat tagtataaca tcatatataa ataaaatcag aggtaaaaga
    13501 tgaaaaaatt aaatttattt atcctacttt tgctaacctt ctttatgttt gcatttggac
    13561 tcactgatat cgttagaaaa attgatttat taagaaatat cttctatccg gaggtttatg
    13621 aaagaa
//
LOCUS       DBNGSV010000296         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_606943, whole genome shotgun sequence.
ACCESSION   DBNGSV010000296 DBNGSV010000000
VERSION     DBNGSV010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_606943"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="purM"
                     /locus_tag="AC159U_10820"
     CDS             complement(<1..563)
                     /gene="purM"
                     /locus_tag="AC159U_10820"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3337582.1"
                     /translation="MAKISYKDSGVNIDAGNEFVEIIKPIVKSTYTKGFTSQIGSFSG
                     LFPFPKGYMNPYLVSAADGVGTKIKIAFMAGRFDTIGIDLVAMNVNDLITCGAKPLFF
                     LDYIATSSINPKQTSSIVKGIAQGCRQSGCVLLGGETAEMPDIYRKDEFDIAGFVVGV
                     VDKKNIIDGKNVKNGDVLIGLASSGLHS"
     gene            complement(585..1172)
                     /locus_tag="AC159U_10825"
     CDS             complement(585..1172)
                     /locus_tag="AC159U_10825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3337583.1"
                     /translation="MSLLSRLFLFIVIAVVGSYVNVAESAAEDKVKKGGNPVVLIKTS
                     KGDITVELDKEKAPISVENFLSYVNDGFYNGTVFHRVIKNFMIQGGGFTTDMKQKPTK
                     DPIKNEAKNGLSNVRGSIAMARTGVVDSATSQFFINHKDNDFLNHGSRDYGYAVFGKV
                     TEGMEVVDAIAEVKTGYQDVPTENIVIESITVVSK"
     gene            1304..>1992
                     /locus_tag="AC159U_10830"
     CDS             1304..>1992
                     /locus_tag="AC159U_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3337584.1"
                     /translation="MPKIDFGVMLRQQKIEFSRIKETAQLCDDLGYHSVWFYDHILGM
                     GGIEVDIYEAWTLMSSLCSVTNDIKLGTMVLCNSFRPPALLAKMGATLDVISGGRLEF
                     AIGAGWFEPEYTSYGYDFPNTKTRIEQLAESVQIIKTMWTEQNPTFSGKYYSIKDAYC
                     NPKPLQTPHPPIQIGGSGEKHLLRVVAEHADEWNCPATAAEHFDSKLKALEGHCEDIG
                     RNIKDIALSQQ"
BASE COUNT          615 a          403 c          344 g          630 t
ORIGIN      
        1 gaatgaagac cgcttgatgc aaggccaatt aatacatcac cgtttttaac atttttaccg
       61 tcaattatgt tttttttatc aactacacca accacaaaac cagcaatatc aaactcgtct
      121 tttctatata tatctggcat ctctgcagtt tctccaccta atagtacgca gccgctctgc
      181 cggcatcctt gtgcaattcc ttttactata cttgatgttt gtttagggtt tattgaagat
      241 gttgctatat agtcgagaaa aaagagaggt tttgcgccgc atgtaatgag atcattaaca
      301 ttcattgcta caaggtcaat acctatagta tcaaaccttc ctgccataaa tgcaatcttt
      361 atttttgtac caacaccatc agctgcggac acaagataag ggttcatata cccttttgga
      421 aaggggaaaa gccctgagaa tgagccaatc tggctagtaa aaccttttgt ataggtgctt
      481 ttaactatgg gttttatgat ttcaacaaac tcatttccgg catctatatt aactccggag
      541 tccttgtatg aaatctttgc catatatgtt gctaaaacaa tatgttattt gcttactact
      601 gtgatggatt caattactat gttttctgta ggcacgtcct ggtatcctgt ttttacttct
      661 gcaatagcgt ctaccacttc cataccttcg gtaaccttac caaaaacagc ataaccatag
      721 tctctgctgc catgatttag aaagtcattg tctttatgat tgataaaaaa ctgagaagtt
      781 gcactatcta ctacgcctgt tcttgccatt gcaattgatc cccttacgtt tgaaagacca
      841 tttttagctt cattcttaat cggatctttt gtaggttttt gtttcatgtc agtagtaaaa
      901 ccaccaccct gaatcataaa gtttttaatt accctatgaa aaacagttcc attgtaaaat
      961 ccgtcattta cgtatgacaa gaaattttca accgaaatag gagctttttc tttatctaat
     1021 tctacagtta tatcaccctt gcttgtttta ataagtacca caggatttcc tcctttcttt
     1081 actttatctt ccgctgcact ctcagctaca ttcacgtatg aacctacaac tgcaataaca
     1141 ataaataaaa ataatctgga taataaactc atataaattc accttccctg taataaaaat
     1201 taaacttaac ttatagtatt gtttaactac tgtcaaacat aaaaggttgc agttaaagtc
     1261 tatttaatgg tataaatatt tagctattca taaggagact taaatgccaa aaattgattt
     1321 cggagttatg ctcaggcagc aaaaaattga attcagcagg ataaaagaaa ccgctcagct
     1381 atgtgatgac cttggatatc actcggtctg gttttatgac catatactcg gtatgggagg
     1441 gatagaggta gatatatacg aagcatggac gctcatgtct tcactctgct cggttacaaa
     1501 cgatatcaag cttggaacca tggtactttg caactctttc agacccccag cgctacttgc
     1561 aaaaatgggt gctacccttg atgtaataag cggaggaagg ctggaatttg caataggcgc
     1621 cggatggttt gagcctgaat atacttctta tggctacgat tttcctaata caaaaacaag
     1681 aatagaacag ctggcagaat ctgtgcagat aattaaaact atgtggacag aacaaaaccc
     1741 tacattcagt ggcaagtatt actctattaa agatgcttat tgtaatccaa aacctctgca
     1801 aacacctcat ccacccatac agattggggg ttcgggagag aaacacttat taagagtggt
     1861 agcagaacat gccgatgaat ggaactgccc tgctacggct gctgagcatt ttgattctaa
     1921 acttaaggct ctggaaggtc actgtgagga tattggaaga aatattaagg atattgcact
     1981 ctctcagcaa aa
//
LOCUS       DBNGSV010000297         1403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1367984, whole genome shotgun sequence.
ACCESSION   DBNGSV010000297 DBNGSV010000000
VERSION     DBNGSV010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1367984"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="AC159U_10835"
     CDS             <1..1055
                     /locus_tag="AC159U_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3337585.1"
                     /translation="VERFLDKPQSEVHRIAKDTLEGNLRGVLATLTPEEVNEDRLKFA
                     ESLISEADNDLRQLGLQLDTLKIQNVSDAASHSRAEVAKAEAAQEIQTAQINTDREVK
                     VREALAGQKIEQENNNLRVIKAELDKVAVVKEEEASVAREKARAEYEQAMEHERIILQ
                     QKRLTADVIEPARAQKEAMELEAKGQAAPILETGKANLEVLNEMIATYKSADGEGEKV
                     FMLNMLPEIISQIVNTVGEVKVDKLSVIDAGGGFNGNGSGLGKAVNQMPVAVLSFIEQ
                     IENATGVNILSQFTGSPKEVQKDESKPKYSITHKAPSRPVKKVVTNPPESDFEDHVHS
                     EPDDTQTKQHDTKTDR"
     gene            1129..>1403
                     /locus_tag="AC159U_10840"
     CDS             1129..>1403
                     /locus_tag="AC159U_10840"
                     /inference="COORDINATES: protein motif:HMM:NF042631.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6559 family protein"
                     /protein_id="HIR3337586.1"
                     /translation="MVTCNIKPDDKKAFIDDMAKSLTNRYGKKKYYSQNEIRNVARYN
                     GYPIDWYCWAYCFFCTHEEFDLIHEQLGEVCDYASMKTELLSEVSGLS"
BASE COUNT          499 a          241 c          334 g          329 t
ORIGIN      
        1 cggtggaaag atttttggac aagccgcaat ccgaggttca caggatcgct aaggatactc
       61 ttgaaggaaa cttaagggga gtgcttgcga ctttaacccc tgaagaggta aatgaagaca
      121 ggctcaaatt tgctgagagt ctgatatccg aagccgataa tgacttaaga cagcttgggc
      181 ttcagctcga taccctcaaa attcaaaatg tatcagatgc cgcatctcac agcagggcag
      241 aggttgcaaa ggcagaagct gctcaggaga ttcagacagc tcagattaat accgatagag
      301 aagtaaaggt gcgagaagca cttgcaggcc agaaaattga gcaagaaaat aataatctca
      361 gggtgataaa ggcagagctt gataaggttg ccgtagtaaa ggaagaagag gcaagcgtag
      421 cgagagaaaa agccagagcc gagtatgaac aggctatgga gcatgaaaga attatacttc
      481 agcaaaaaag acttactgct gatgtaatcg aacccgcaag agcacaaaaa gaagctatgg
      541 agcttgaggc taaaggtcag gctgctccaa tccttgagac tggtaaagca aatctcgaag
      601 ttttaaatga aatgatagca acatataaga gtgcagacgg tgagggcgaa aaagtgttta
      661 tgctcaatat gcttcctgaa atcatttcgc agatagttaa cacagtaggt gaagtaaagg
      721 ttgataaatt aagtgtaatt gatgcagggg gtggctttaa cggaaacgga agcgggcttg
      781 gaaaagcagt taatcagatg ccggtagcag ttctatcatt cattgaacag atcgagaatg
      841 caacgggtgt taacatttta agtcagttta caggcagtcc gaaagaagta caaaaggatg
      901 agtctaaacc caaatactcc ataacacata aagctccctc aagaccggta aaaaaagtgg
      961 taactaatcc acctgaatca gattttgaag accatgtgca ttctgagccg gatgatacac
     1021 aaactaaaca gcatgataca aaaacagaca gatagtcctg aataaatcat aaactaaagc
     1081 ctcttagaag attaaggggc tttagtttta atataggagg tgtcagttat ggtcacatgc
     1141 aacattaagc cggatgataa aaaagccttt atagatgata tggcaaagag ccttacaaat
     1201 cgatatggta aaaagaaata ctactcacag aatgaaataa gaaatgtagc aagatataac
     1261 ggttacccca ttgattggta ctgctgggca tactgctttt tttgtacaca tgaagagttt
     1321 gatcttattc atgagcagct tggtgaagta tgtgattatg cctctatgaa aactgaacta
     1381 ctttcagaag tttccgggtt atc
//
LOCUS       DBNGSV010000298        10048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_953986, whole genome shotgun sequence.
ACCESSION   DBNGSV010000298 DBNGSV010000000
VERSION     DBNGSV010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_953986"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..413)
                     /locus_tag="AC159U_10845"
     CDS             complement(108..413)
                     /locus_tag="AC159U_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3337587.1"
                     /translation="MDKIKKLINHFDSFGDVLLFFQILIFLTILPVLLKVFSLKKLMG
                     ILSSDSKEAQIKDRAEKVLELTNLVLYRDFWIYKNICLKRSLALYYFLSRMGHEVNI"
     gene            499..1668
                     /gene="bioF"
                     /locus_tag="AC159U_10850"
     CDS             499..1668
                     /gene="bioF"
                     /locus_tag="AC159U_10850"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HIR3337588.1"
                     /translation="MADLLSWIEKELDVIKDKDLFRILTELESAQSPEIIIDGKKYIL
                     LSSNSYLGLTVEPRVIQAAVEATEKYGTGSGGSRLVSGSTDLHRQLEQRLADFKKTEA
                     AILFSTGYLANIGTISTLVGKDDIVYSDELNHASIIDGCRLSKADIRIYKHLDVNHLE
                     SLLEKDMNRKCKKLIVTDTVFSMDGDLAPLPEIADLADKYGCMFMIDEAHSTGVLGKR
                     GSGGTEHFGIEDRVPIVMGTLSKAVGSLGGYIAGSQKLIDFIRNRVRSYIFDTSLPAA
                     SLAASLAAIDIIEYEPERREYLWKLINKFKQGLDDIGLTVLPSHSAVIPVLIGEAQPT
                     LDFAKSLRENGVYTPAVRPPSVPDGMCRIRATLMAKHTEEQIDHALKAFKLSLNN"
     gene            1792..2298
                     /locus_tag="AC159U_10855"
     CDS             1792..2298
                     /locus_tag="AC159U_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3337589.1"
                     /translation="MKEKLKMANIINEKDLVWDTNSYNSRFEYKKKSLSQNSGAEKLG
                     CSIYEVPPGKSAFPFHYHCSNEEAVFILEGNAELRFGDESYFVSKGDYLTFPAEGSAH
                     QLTNRGETTLKYICISTMVEPDIKVYPDSGKVGVVSTASNNGTANSGKTEKFLRGDVE
                     VVGLWEGE"
     gene            2425..3243
                     /gene="lsrF"
                     /locus_tag="AC159U_10860"
     CDS             2425..3243
                     /gene="lsrF"
                     /locus_tag="AC159U_10860"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HIR3337590.1"
                     /translation="MMDWGLANRMARIINPKTGRAVMLAFDHGYFLGPTHRIEEPRKT
                     IEPLLPYADTIMLTRGVLRTCVDPESSANVVLRVSGGVSIVGPALEDEGITTSIKDAL
                     RLNVAGIGISVFVGSSHERQTLLNLSELVNQGQEYGVPVLGITAVGKELEKRDARYLS
                     LCCRICAELGTHIVKTYYCEDFEKVTTSCPAPIVIAGGPKLETELDVFELTYNALQQG
                     AVGVDMGRNIWQNDNPVAVLRAIRGIVHEGLKPKQAHKLYLEVKSENEKAAANA"
     gene            3298..4323
                     /locus_tag="AC159U_10865"
     CDS             3298..4323
                     /locus_tag="AC159U_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HIR3337591.1"
                     /translation="MRVAMYYNNSDIRIENVSQPVLQSGEILLRTMASGVCGSDTMEW
                     YRVPKAPIVLGHEVAGEIVEVRGDVKNFKPGDRVIATHHVPCNTCYHCLRGNHSACDT
                     LRSTHFDPGGFSEYIRIPSINVDRGVLRLPDNVSYEQGSFVEPLGCVVRGQRLAGFEA
                     GSCVLIIGCGMTGLLNLQYAKAQGAGTIFASDINDFKLRTAKKLGADHVLRPDISIGE
                     NIKELNEGRLADFIVVNTGAPSAIKQALSLIELGGTILYFAPSDPGYKLTIDFNEIWW
                     KGIKIISSYAASQADLSVALNMISSGRVNVAEMITHRLPLSQTQKGFELAIKSDDSIK
                     VIIEPQS"
     gene            4447..6897
                     /locus_tag="AC159U_10870"
     CDS             4447..6897
                     /locus_tag="AC159U_10870"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HIR3337592.1"
                     /translation="MAELKHTEVYKKCDLSNLGFKNTDEIGSCDQLIGQDRAVRALNF
                     GLGMNNRGYHIYIAGRSGVGKSTSVKHMLESISKDKPQPFDWCYVYNFIDPTEPKAFT
                     LPTGTGKLFKKDMEDFIELLKENLPKAFETKEYEEERQNLTNIFQRDKNILFDNLQEF
                     AKTEEMQVQFTPTGIVTIPIVGGKPITQEDYAKLDDSFKEIIRKRKEVVENEISSVVK
                     KVRKMDQEFMDKVKELEQKVALFAVQNIVDNLREKYTDKSQILEYLAEVQKHILDNIY
                     TFLPDRGSNAPPGFPLAMPKPEPTFTEYKVNVFIDNSNTEGAPVIFETHPTYTNLFGS
                     IEREARFGALVTDFTMIHAGSIAKANGGYLVVEANDLFKYPLVWDSLKKVLENRELRI
                     EDVSQHYGLMSTMGMRPEPMELNVKVIIMGNPQIYNLLYTYDEDFRKLFKVKADFDSV
                     VKKDDDILYKYCCLIKSIGEKENLKTFDRSGVEAILEYSTRLAGDQDKLSVQFGAISK
                     IIKEANYWSEVNGNNKNIKRADVERAIDEMIYRSNMIEEKIREMITDGNIMISTSGQT
                     VGQINGLAVYNMGDYAFGKPNRITCETYIGAEGLINIERRAKLSGNIHDKGVLILNGY
                     LGAKYAQDIPLSLSASLGFEQTYEMIDGDSASAAELVALLSSLSEIPIKQCFAITGSV
                     NQKGQIQPIGGVNEKVEGFFHVCKEKGLNGENGVLIPHQNVQNLMLKKEVIEAVKNKK
                     FHIHSIETIEDALQILTGTPAGQMLSDGSYKEGTVNYLVSKKLKQLAEEYRKSVKGAK
                     NTSGGGQDSENNSEELSS"
     gene            6910..8274
                     /gene="radA"
                     /locus_tag="AC159U_10875"
     CDS             6910..8274
                     /gene="radA"
                     /locus_tag="AC159U_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3337593.1"
                     /translation="MKRSKATVIFVCQNCGNNSPRWIGKCPACEQWNTFVEEIGTKEK
                     PSPVLNPKSKPVLINEISSENEQRIKTDIAEMDRVLGGGLISGSTVLIGGDPGIGKST
                     LALQIFGKYSENGIKVLYVTGEESSNQIKIRAKRLNVNGDNLYVVTETNIDSIINNTE
                     KNDPGVVVIDSIQTIYSADLPSAPGSVGQIRECTSKIVQYAKNKGISFFLIGHVTKEG
                     SIAGPKVLEHLVDTVLYFEGGKSHPYRILRAVKNRYGSTNEIGVFEMTNDGLEQVTNP
                     SEIFLSERPLNSSGSVVSPSIEGTRPILVEIQALVSPCNFGVPRRTTIGLDQGRVSLL
                     VAVLDKRAGLHITSQDIFMNVAGGVKIEEPAVDLGICISLVSSFLNKPVSAETIVFGE
                     VGLSGEIRGVSQVDVRLKEAQKLGFKRFVLPKINYDNQDFKKSTQKYIGIENIGEAIE
                     ILFD"
     gene            8319..9137
                     /gene="murI"
                     /locus_tag="AC159U_10880"
     CDS             8319..9137
                     /gene="murI"
                     /locus_tag="AC159U_10880"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR3337594.1"
                     /translation="MNNQKQQLPIGIFDSGIGGLTVVKEIRKRLPNENIIYLGDTARV
                     PYGTKSSSTVISYSLSNAAFLSSHNIKLLVVACNTASAFALDSLKSTLDIPVIGVLEP
                     GALKAVETTRNNVVGVIGTPSTIKSEAYREAVNKIGPNITVHTKSCPLFVPLAEEGWI
                     DDPISEQVAVRYLKPLINKKIDTLILGCTHYPLLKKLIQRVVGDDIKLVDSAEQVTTQ
                     TLSILEELGIKNPENKSGNKQFYLTDVSDSFLSIAASFLGEQVSGIKQIDIKSA"
     gene            complement(9139..9837)
                     /locus_tag="AC159U_10885"
     CDS             complement(9139..9837)
                     /locus_tag="AC159U_10885"
                     /inference="COORDINATES: protein motif:HMM:NF047036.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6901 family protein"
                     /protein_id="HIR3337595.1"
                     /translation="MGSIDICYRFKLDDNEEEVFDLKFNEDTFELISDGNERLPLWSK
                     LDYHKCPHCPLDSKTVPNCPVAKNFSNVVLRFDHIFSFHEIEIEVITKERTVSSKTTA
                     QRGISSLLGLIFAASGCPHTSLFRPMARFHLPLSTEQETIFRVTGMYLLAQYLQDKEG
                     KNASFDFDGLKEMYNNLHLLNTKIADRIRDAVTTDSSVNGIILLDMLTKLMPIAIDRS
                     LEDIKGLFSQYNKE"
BASE COUNT         3402 a         1819 c         2068 g         2759 t
ORIGIN      
        1 aacatttgta ttagcttgct tatcttacta tttgatagtt ttgtattctt aagtaacttc
       61 ttttgcatag tttcagctta gtatactttg ctgtcctatg cgagctccta aatatttact
      121 tcatgcccca tcctgcttaa aaaataatag agcgcaagtg agcgtttaag gcatatattt
      181 ttgtatatcc aaaaatcccg ataaagtaca agattagtaa gctcaaggac tttttctgct
      241 ctatctttaa tctgagcttc tttagaatcc gaactcagaa ttcccatcaa ctttttaaga
      301 gagaatactt ttaggagtac aggaagaatt gttaaaaaaa taagaatttg gaaaaataat
      361 aaaacatccc caaatgagtc gaagtggtta attagttttt taattttatc cataatgtta
      421 ctacttgttt gttagtatat agcctcatgc aaacattttc tatattgaac tactccctgg
      481 ttcgtggtta tctataaaat ggcagattta ttatcctgga tcgaaaaaga attggatgta
      541 ataaaggaca aagacctgtt tagaattcta acagagcttg agtccgcaca gtctcctgag
      601 atcataattg acggcaaaaa atatattctc ctatcatcca acagctactt gggactaaca
      661 gtagagccaa gagtaattca ggcagcggtt gaagccactg aaaaatacgg cacaggctca
      721 ggaggctcaa ggctagtaag cggcagcacc gacctgcaca ggcagcttga gcagcggtta
      781 gcagatttca aaaaaaccga agcagcaatc ctctttagca ctggatacct tgctaatatt
      841 ggaacaatat caacattggt gggaaaagat gacattgttt acagtgatga gctaaaccac
      901 gcatcaatca tagatggatg cagactttcc aaggcagata tcaggattta taaacatctg
      961 gatgtaaacc acctggaatc tttactggaa aaagacatga acagaaaatg taaaaagtta
     1021 attgtgaccg atactgtgtt cagtatggat ggtgaccttg cacccctgcc cgagattgcc
     1081 gacctagcag ataaatatgg ctgtatgttc atgatagatg aggctcattc tacaggcgtg
     1141 cttggaaaaa gaggaagcgg cggcacggag catttcggca ttgaagatag agttcctatt
     1201 gtcatgggca cactatcgaa agcagtcggt tccctgggag gatatattgc cggaagccag
     1261 aaattaattg attttattag aaacagggta cgtagttata ttttcgatac atcattacca
     1321 gctgcatctc ttgccgcatc tttggcagca attgatatta tagaatatga accggagaga
     1381 agagaatatc tgtggaaatt aataaacaaa ttcaaacaag gtctggatga tattggactt
     1441 acagtgctgc catcacattc tgcagtaata ccggttttaa taggtgaagc tcaaccaaca
     1501 ctcgattttg caaaatcgct aagagaaaat ggggtttata ctcctgcagt caggccgcct
     1561 tctgtacctg atgggatgtg cagaataaga gctacactta tggcaaaaca taccgaagaa
     1621 caaatcgatc atgcactaaa agcattcaaa ctaagtctca acaattagaa ccctcctgta
     1681 tttatttgat aacaataatt taattatttt tttcaataag catattgaaa gatttatttt
     1741 ttatgcaata ctaaagcatt atagttgctt tagtattaag gagcatggaa aatgaaagaa
     1801 aagcttaaga tggccaacat cataaatgaa aaagatcttg tatgggatac taacagctac
     1861 aattccaggt ttgaatataa aaagaaatca ctttctcaaa actctggcgc cgagaaactg
     1921 ggctgctcaa tctatgaagt accacccgga aaatctgcat tcccatttca ttatcattgc
     1981 tcaaatgaag aagctgtatt tattttagaa ggaaatgccg aactcagatt tggtgatgag
     2041 tcatactttg tatcaaaagg tgactacctt acttttcctg cggaaggatc cgcacaccag
     2101 ctcacaaata ggggagaaac aacactcaaa tatatatgta tatccacgat ggttgaacct
     2161 gacataaagg tttatccgga ttccggtaaa gttggagttg tgagtacagc ttcaaacaat
     2221 ggaacagcaa atagtggtaa gacagaaaaa ttcttaagag gcgatgttga agtcgtgggt
     2281 ctttgggaag gcgaatagtt cttttatccc aattttacat cttacaatta gatcttgtta
     2341 atctttataa gtctgaattg acttaaagcc ggccatccat taaatttcta tctctaacaa
     2401 aataaattca agagaggtat aacaattatg gactggggac ttgctaacag aatggcccgc
     2461 ataataaacc caaagaccgg tcgagcagta atgctagctt ttgaccacgg atactttttg
     2521 ggcccgacac acagaataga agaaccaaga aaaacaatcg aaccgctttt gccatatgca
     2581 gacaccataa tgcttacccg tggtgtgctt agaacctgtg tagacccgga atcgagcgca
     2641 aatgtggtgc taagagtatc tggcggagta agcatagtag gtccggcgct agaagatgag
     2701 ggtattacaa cctctataaa agatgctctt aggctaaatg tagccggtat cggaatatcg
     2761 gtatttgtag gctcatctca tgaaagacag acgcttttaa acctttcgga gcttgtaaac
     2821 caggggcagg aatatggagt tccagtgctt ggtattaccg cagttggaaa ggagcttgaa
     2881 aagagagacg cccggtacct ttcgctttgc tgcagaattt gcgcagagct tggaacacat
     2941 attgttaaaa catattactg tgaagatttt gaaaaggtca ctacaagctg tcctgctcca
     3001 atcgttattg caggcggacc caagcttgaa actgaattgg atgtatttga gctaacatat
     3061 aacgcattac agcagggtgc cgtcggggtg gacatgggaa gaaacatctg gcaaaacgat
     3121 aaccccgttg ctgtgctaag agccatacgc ggtatagtac acgaagggct taaaccaaaa
     3181 caggctcaca aattgtacct agaagtaaaa tcagaaaatg aaaaagctgc tgctaatgct
     3241 tagtatcttt tcaggcaatc aactatgtat gccactatgg agtaaacgga gtaaacaatg
     3301 cgcgtagcaa tgtattacaa caactctgat atacgtatag aaaatgtctc tcagccagta
     3361 ttgcaatccg gtgagattct attaagaacc atggcaagcg gtgtttgcgg tagtgacaca
     3421 atggagtggt accgcgttcc aaaggcccct attgtgcttg ggcatgaagt cgcaggagaa
     3481 atagtcgaag taagaggcga tgttaagaat tttaaacctg gagacagggt cattgccaca
     3541 catcatgtcc catgcaatac atgctatcac tgcctgagag gcaatcactc tgcttgcgac
     3601 actctgagga gtactcactt tgatcccggc ggattttcag aatacatcag aatccctagt
     3661 attaatgttg accgcggggt gctcaggctt cccgataatg tttcctatga acagggctca
     3721 tttgtagaac cgcttggctg tgtagtacgc ggtcaaagac ttgcaggatt tgaagccgga
     3781 agctgcgtcc taataattgg atgcggcatg acagggcttc taaatttaca gtatgcaaaa
     3841 gcacagggtg cagggacgat attcgcttcc gacattaatg actttaaact cagaacagca
     3901 aagaaactag gtgcagatca tgtacttaga cccgatataa gcattggtga aaacataaaa
     3961 gaactcaatg aaggaaggct tgcagacttc atagtagtca acaccggtgc gccatctgcc
     4021 atcaagcagg cattatcact aattgagctt ggggggacca tcctgtactt tgctccttct
     4081 gaccccgggt ataaactcac tattgatttc aatgagattt ggtggaaagg aattaagatt
     4141 atcagctcct acgccgcttc acaggcagat ctctcggtag cgcttaacat gataagcagc
     4201 ggcagggtga atgtagccga gatgataacc cacaggctac ccctatccca gacacaaaaa
     4261 ggttttgaac tggctataaa atcagatgat tctatcaagg tcattataga gcctcaaagc
     4321 tgatctcaac tgcttatttt tattaaattt aaaaataacc ttttttattt tgtataactc
     4381 tattacaata tgagtcatta cttagtaata ttacagtaaa tctcaatatt tcattaggag
     4441 tagaatatgg ctgaacttaa acatactgaa gtatataaga aatgtgacct ttcaaatctg
     4501 ggattcaaaa atacagatga aataggcagc tgtgatcagt tgataggaca agacagagca
     4561 gttagagcgc taaattttgg actcggtatg aataacagag gttaccatat ctatattgct
     4621 ggaagatcag gtgttgggaa atctacatcg gtaaaacata tgcttgaatc tataagcaag
     4681 gataaaccgc agccattcga ttggtgctat gtgtataatt ttatagatcc aactgagccg
     4741 aaagccttca cacttcccac aggtacagga aagctcttca aaaaagatat ggaagatttc
     4801 attgagctac taaaagaaaa ccttcctaaa gcattcgaga caaaagaata tgaagaagaa
     4861 agacaaaatt taaccaacat attccaaaga gataaaaaca ttctttttga taatctgcag
     4921 gaatttgcaa aaactgaaga aatgcaggtt cagttcactc ctacaggaat cgttaccata
     4981 cccattgtcg gcggcaagcc aataacacag gaagattatg ccaagctaga tgattcattt
     5041 aaggaaatca taagaaaacg aaaggaagta gttgaaaacg aaatctcttc tgttgtaaaa
     5101 aaagtacgca agatggatca ggaatttatg gataaggtaa aagagcttga acaaaaagta
     5161 gccttgttcg ctgtacagaa tatagttgac aatttaagag aaaaatacac cgacaaatca
     5221 cagatccttg aataccttgc agaggtacaa aaacacattc tcgataacat atacactttc
     5281 ctgccagaca ggggttcaaa tgcaccaccc ggatttccat tagcaatgcc aaagcctgaa
     5341 cctacattta ccgaatacaa ggtcaatgta tttatagata actccaatac cgagggggcg
     5401 cccgtaatat ttgaaactca tcctacttat acaaaccttt ttggatcaat tgaaagagag
     5461 gcgcgatttg gagcgcttgt tactgatttc acaatgattc acgcaggatc gattgcaaag
     5521 gcaaacgggg gctacctggt tgtagaagct aatgacttat ttaaataccc gcttgtatgg
     5581 gactctctta aaaaagtttt agaaaatcgg gagcttagaa tcgaagatgt ttcgcagcat
     5641 tacggcctta tgagcactat gggaatgaga ccagaaccca tggagttgaa tgtaaaagtt
     5701 ataataatgg gcaatccgca aatctacaac ttgctttaca cttacgatga ggattttaga
     5761 aaacttttta aggtaaaagc agactttgat tctgttgtaa aaaaggatga cgatattctt
     5821 tataaatatt gctgccttat aaagtctatt ggtgaaaaag aaaatctaaa aacatttgat
     5881 aggagcggtg ttgaagccat tttagagtat tccacaaggc ttgcaggaga ccaggacaag
     5941 ctctctgtgc aatttggtgc aatttcaaaa ataattaaag aagctaatta ctggagtgag
     6001 gttaatggca ataacaagaa catcaaaaga gcagatgttg aaagagcaat agacgaaatg
     6061 atctacagat cgaatatgat cgaagagaaa ataagggaaa tgattactga cggcaatatc
     6121 atgatttcta catcaggaca aaccgttgga cagataaacg gtcttgctgt ctataatatg
     6181 ggcgactatg catttggaaa gcccaacaga ataacatgtg aaacatatat tggggctgaa
     6241 ggtcttatta atatagaaag aagagcaaag ctaagcggaa atattcatga caaaggtgta
     6301 ctgattttaa atggttatct tggagcaaaa tatgctcagg acataccctt gagtctatct
     6361 gcaagtctgg gatttgagca gacctatgag atgattgacg gagacagcgc atctgcagca
     6421 gaacttgtag cacttctctc aagcctgtct gaaattccca ttaaacagtg cttcgccatc
     6481 acgggttcag taaaccagaa agggcagatt cagccaatag gtggagtaaa tgaaaaagtg
     6541 gaaggatttt tccatgtttg taaagaaaag ggattaaatg gagagaatgg agtacttata
     6601 cctcatcaaa atgttcaaaa tttaatgctt aaaaaagaag taatcgaagc tgtaaaaaac
     6661 aagaaatttc acatacattc tattgagacc atagaggatg ccttgcaaat tcttacaggc
     6721 actcctgctg gacaaatgct ttcagatggc agctataagg aaggcactgt aaactatctt
     6781 gttagcaaga agcttaaaca actggccgaa gagtatagaa aatccgtgaa aggtgcaaaa
     6841 aatacctcgg gtggaggaca agactcggaa aataattcag aagagctaag ctcatgagtt
     6901 catcttaata tgaaaaggtc caaagccact gtaatatttg tttgccaaaa ttgcggtaac
     6961 aactcaccaa ggtggatagg gaagtgccct gcatgtgagc agtggaatac gtttgtagaa
     7021 gaaataggta ccaaggaaaa accaagtcct gttctgaatc ccaaatcaaa accagtatta
     7081 attaatgaga tttcatcaga aaatgagcag cgtataaaaa ccgatattgc cgagatggac
     7141 agagttctcg ggggtgggct gatctctggc tctactgttc taataggtgg agatcccgga
     7201 ataggaaaat caacactagc cctgcagatt tttggaaaat attctgaaaa cggaataaaa
     7261 gttttatatg taactggaga agaatcgtcc aaccaaataa aaattagagc aaaaagatta
     7321 aatgtaaatg gggataacct gtatgtggtc acagagacaa acattgacag cataattaac
     7381 aataccgaaa aaaacgatcc tggtgtggtt gtaatagatt ctattcaaac gatatactct
     7441 gcagaccttc catccgctcc cggatctgta ggtcagataa gagaatgcac atctaaaata
     7501 gtgcaatatg ccaagaacaa aggtatttcg ttttttttaa taggacatgt aacaaaagag
     7561 ggttcaattg caggccccaa ggtactggaa catctggttg acactgtact ctattttgaa
     7621 ggcggcaaat ctcatcctta tagaatttta agagccgtaa aaaaccgata tggctccact
     7681 aacgaaattg gtgtatttga aatgacaaat gacggtctgg agcaggttac caatccatcg
     7741 gaaatctttc tttctgaacg cccgttaaac agctcgggat cggttgtttc tccgagtata
     7801 gagggtacaa gacccatact cgtagaaatt caggcacttg tatcaccatg taattttggt
     7861 gttccaagaa gaacaactat cggacttgat cagggcagag tctcgctttt agtagctgtg
     7921 cttgataaaa gagccggact tcatatcact tcacaggata ttttcatgaa tgttgcaggc
     7981 ggtgttaaaa tcgaagaacc cgcggttgat ctgggtattt gcatttcact ggtttcaagt
     8041 tttttgaata agcctgttag tgcagaaacc attgtatttg gcgaggtagg actatcagga
     8101 gagattagag gcgtatctca ggtcgatgtg agattaaaag aagctcagaa gcttggattc
     8161 aaaagattcg tattacctaa gataaactat gataatcaag actttaaaaa atctacgcaa
     8221 aaatatatcg gcatcgagaa tataggcgag gcaattgaaa tattgtttga ttagatataa
     8281 attaatccag ccagtctgca actattattt atatttcaat gaataatcaa aaacaacaat
     8341 tgccaatagg aatttttgat tccggcatag gcggtcttac cgtagttaaa gaaattagaa
     8401 agagacttcc taatgaaaat attatatatc tgggagacac ggctagagta ccatacggca
     8461 caaaatccag cagtacggtt atctcctact cacttagtaa tgcagcattc ctaagttcgc
     8521 acaatataaa gcttttagtt gtggcctgta acacagcttc agcatttgca cttgacagcc
     8581 taaaatctac tttggatatt ccggtaattg gtgtactgga gccgggagct ttaaaagctg
     8641 tagaaactac gagaaacaat gtggtgggtg taattggtac accttcaacc ataaagagtg
     8701 aggcatatag agaagccgta aataaaatag gtccaaacat aaccgtacac actaaatcct
     8761 gtccgctctt tgtaccactt gctgaggaag gatggattga tgatcctata tccgagcaag
     8821 ttgccgtaag atatctgaaa cccttaatta acaaaaagat tgatactcta attcttggat
     8881 gtacacacta tcctctgctt aaaaaattaa ttcaaagagt tgtaggagat gatattaaac
     8941 ttgttgattc agctgagcag gttactacgc agaccttaag cattcttgag gaattgggta
     9001 ttaaaaatcc ggagaacaaa tcaggaaata aacagtttta cttaactgat gtatcggata
     9061 gttttttatc tatcgcagca agctttcttg gcgagcaggt tagcgggatc aagcagatag
     9121 atataaaatc tgcataattt attctttatt gtactgagag aataaaccct taatatcctc
     9181 taaacttcta tctatggcaa tcggcataag ctttgttagc atgtccagaa gtataattcc
     9241 atttaccgaa gaatctgttg taacagcatc tcttatacga tctgctattt ttgtatttaa
     9301 aaggtgcaga ttattataca tttcttttag accatcaaaa tcaaatgatg catttttacc
     9361 ttctttatcc tgtaaatact gagcaaggag atacatacca gttaccctga atattgtttc
     9421 ctgttcagta gaaagaggga gatgaaacct tgccattggt ctaaagagtg aggtatgagg
     9481 acagccgctt gcagcaaata ttaatcccag caatgagctt attccacgct gggctgttgt
     9541 ttttgatgat actgttctct cttttgtaat aacttctatt tcaatctcat ggaatgagaa
     9601 tatatgatca aatcgtaaaa ccacatttga aaaattcttg gcaaccgggc agtttggaac
     9661 tgtcttagaa tcaagtggac aatggggaca cttatgataa tcaagcttgg accaaagcgg
     9721 aagcctctca ttaccatccg aaattaattc aaaggtatcc tcattgaatt ttaaatcaaa
     9781 aacctcttct tcgttatcgt ctaatttaaa gcgataacat atatctattg atcccatata
     9841 ccaattatct ctataatgat aaaaatgtcc aaaaaattta gataattata ctatttttaa
     9901 taacaaaaat ttaattaaga aaataaaata gctattaaat gaaaaatact aataaactta
     9961 agcttaattt taggaaaaaa ttaatgagat tagttaaaat atttggctcc ggaggagggg
    10021 ctcgaacccc cgacctagtg attaacag
//
LOCUS       DBNGSV010000299         7086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1507647, whole genome shotgun sequence.
ACCESSION   DBNGSV010000299 DBNGSV010000000
VERSION     DBNGSV010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1507647"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..598
                     /locus_tag="AC159U_10890"
     CDS             314..598
                     /locus_tag="AC159U_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337596.1"
                     /translation="MDKVWDFYTPAQILAVQGMQKGGKDYPFVNLRLAVANLVRIESN
                     QGGLAQREKTLKNTITAKRKPTMPPPKKKTIPAPPVCEFPMGNPRCAVCD"
     gene            591..2039
                     /locus_tag="AC159U_10895"
     CDS             591..2039
                     /locus_tag="AC159U_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337597.1"
                     /translation="MTDDIQVQNDIKPGGKMSIPRDTNEQFENAVLGSILIDPKIMDE
                     VSAVLTPEDFYRTGNKRLYEVLKDMHENAVPIDLMTVLERAREQKSESTIGGPQRLIY
                     LTETTPASANFAFYVNKVKEASRRREFVDTFAGLSKEVEQGTLSVPEAWGLVAEIEWS
                     MPVRSGLRPVCGSDITVQKDSVQTLWGEVIYPQCITQINSEPGVGKTTFAYNLCATGA
                     EGGEFLGISFSKSLKVLYIDVETPAWKRALKLTSICDGKIPARLYFLDSLDLHSQFSE
                     LLSLCKTSGYDLIVFDTQSRIFNLESENDNAEANRMMLLIRRLVTDAGAAVVLVHHTC
                     KSDKKGVYSGRGASAIGAAVDVVVNMEAMDNDSLKLKVDKNRINGDYMTLFIKKSGDD
                     CFEPFVPPAETLSGFEKFRVQEFILGLDGEKPVWTTSDLQCECSKQGFSRNTITRAIS
                     DLAQSGRINKIRRGEYEIIHKTHNPKTTGAIH"
     gene            2208..3275
                     /locus_tag="AC159U_10900"
     CDS             2208..3275
                     /locus_tag="AC159U_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010478431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR3337598.1"
                     /translation="MRARTCVFILICFLVIQSHGFAQHHSSHESDHMDSANSSTHHHS
                     LRPDEYPPVGVMGGHTHHKGGWMMSYRMMYMSMDGNRSGTNDLSTADVLQDFMVTPTD
                     MDVWMHMFGLMYAPTDNLTVMGMIPYASKSMNHVTRMGGSFSTKTDGIGDIKLSALYK
                     IFENNGHQFHINAGISFPTGDTDERDDLPTGPNQPLPYPMQLGSGTFDLMPGLAYLGQ
                     HHMWSWGAQVLGVIRLGENDEDYTLGNVLSSTVWGSRNINNWISTATRLNWKIWGNIN
                     GADPRLNPAVVPTADPDRRAGNRLDLLFSLNFLIPKGPQLIKGQSLAVEFGLPVYQWL
                     DGPQLETDWLVTFGWRYDWEF"
     gene            complement(3296..3757)
                     /locus_tag="AC159U_10905"
     CDS             complement(3296..3757)
                     /locus_tag="AC159U_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3337599.1"
                     /translation="MSIKLTSPAFENGSMIPVRYTCDGNDISPRLNWENIPDGTESIA
                     IIMDDPDAPRGTWVHWVIYNLSPDADELPENIATEETVQDGAIQGKNSWGTIGYGGPC
                     PPGGVHRYFFKIYALDSGPPAHPGATKEQLLESMSGHILDEGQLMGKYERA"
     gene            complement(3898..4254)
                     /locus_tag="AC159U_10910"
     CDS             complement(3898..4254)
                     /locus_tag="AC159U_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HIR3337600.1"
                     /translation="MAESKSVTLAVMKPKEISEFSDTALSILWDDGHESIYLYEDLRK
                     YCPCASCNELRKASQKTSSFKRSIPVNVSTPDIRPINAEHIGKYAIRFYWSDRHSTGI
                     YTFDFLRELCSCERCN"
     gene            complement(4241..5050)
                     /locus_tag="AC159U_10915"
     CDS             complement(4241..5050)
                     /locus_tag="AC159U_10915"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3337601.1"
                     /translation="MSKHQFFNIRKNDGVLVIAHRGGPGLRPENTITAFKNAQQLGAD
                     VIELDIHSSKDGEIVIMHDDTIDRTTNGSGNVNNYTLSELKEFDAGYNWTDDDGKTFP
                     FRGKGIRIPTLSEVISEIKDSAIIIEIKQSEPSLIKPLSELIIKHGIQNRAIVASFSN
                     KALLDFRSIMPKVATSTTKSEAVKFFFLNQFYLAGFYNPSPAVALQVPEHYKGKKVIT
                     ERFVSTAKKQNLEVHAWTINEPEDMKRLIDLGVEGIVTDYPDRLLKLLGRI"
     gene            5303..5995
                     /locus_tag="AC159U_10920"
     CDS             5303..5995
                     /locus_tag="AC159U_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HIR3337602.1"
                     /translation="MKIAISGLDLEEGKIKYEDPVFKGLCDKFEPAKQAPYYFELRHD
                     DYQAADAIAITKDSVLDLLILDIEKLENRIERAESEEEKAVLKRCLELLENEQPVCDL
                     EITDEERSILTTISPLSFKPVLVLEDSNIDANTVCKRMLDKAGMMFFYTAGKQEVHAW
                     IVEKGTDAVTCAGRIHSDLARGFIKAELISFDDLMAAHSFADASSKGLTNLVDKDFPV
                     PENTILEIRFNV"
     gene            6043..6219
                     /locus_tag="AC159U_10925"
     CDS             6043..6219
                     /locus_tag="AC159U_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337603.1"
                     /translation="MQNIKDHKEVLDSIALLKILAMSSKSIKEGRVKTAKKAFSDIRK
                     SIEKDKKSSSLSSK"
     gene            complement(6225..6632)
                     /locus_tag="AC159U_10930"
     CDS             complement(6225..6632)
                     /locus_tag="AC159U_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR3337604.1"
                     /translation="MNYWLVKSEPFKYSWDDFIKDGSTYWDGVRNYQARNNLKAMKIK
                     DRVLYYHSNEGLEVVGVAEVIKEFYQDPTTDDDRWVVVDLKPAGALSSPVTLKQIKAE
                     PSLKDIALIKNSRLSVMPVTKKEFDKILKMSQS"
     gene            6718..>7086
                     /locus_tag="AC159U_10935"
     CDS             6718..>7086
                     /locus_tag="AC159U_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337605.1"
                     /translation="MIIKYCTVCLLITFIYSCVPAPVGVDTAGIDAKNKTIAILPFGL
                     EKDNEALQVPEGYLQRFNHSMHESLFNSLHSTLVIDLNKSYEILRNTESYDNSFAEFS
                     AQSGADYILTGFVSHYKQRVG"
BASE COUNT         2060 a         1447 c         1618 g         1961 t
ORIGIN      
        1 tacaatcacc aaagacctca ccagtctctg gggggagtac caccagtaga gttcctaaaa
       61 aactgtcctc gaattacgtg acatctacag caatgacggg gtggagtgga gcattaagtt
      121 ttcagcgctt tttttttgct gattttcacc aaacacactg agcctgtcga agtgtgatgt
      181 gcattgaatt catatgacat atacgcattt cgactaagct caatgcgtta ggtcactact
      241 tttaacacac tttcccaaaa aaccaaaatg ggagagctgt gtccaaacca cttttaccgg
      301 gaggtaacaa cagatggata aagtatggga tttctatacc cctgctcaaa ttctggcagt
      361 gcagggcatg caaaagggcg gcaaggacta cccgtttgta aacttaaggc ttgcagtcgc
      421 aaaccttgtg cgaatagaaa gcaatcaggg cgggcttgcc caaagagaaa aaacccttaa
      481 aaacacaata acagcaaagc gtaaacccac catgcccccg cctaagaaaa agaccatacc
      541 agcaccgcct gtgtgcgaat ttcccatggg aaatcccaga tgcgcagtgt gtgactgatg
      601 atatacaggt tcaaaatgat attaaaccgg gaggaaaaat gagtatacca agggatacga
      661 acgaacagtt cgaaaatgca gtgctggggt cgatattgat tgaccctaag ataatggacg
      721 aagtaagtgc ggttttaacg ccggaagact tctacaggac aggcaataaa aggctgtatg
      781 aggtgctaaa ggacatgcac gaaaatgcgg tgcccatcga tcttatgacg gtgcttgaaa
      841 gggcaaggga acaaaagtca gaaagcacaa taggcgggcc gcagaggctt atttacctaa
      901 cagaaacaac gcctgcttca gcaaactttg cattctatgt aaacaaggta aaggaggcgt
      961 cgcgcaggcg cgagtttgtt gatacatttg ccggtctttc aaaggaagtg gagcagggga
     1021 cactcagcgt acccgaggca tgggggcttg tagcagagat tgagtggtca atgccggtgc
     1081 gctcgggcct caggcccgtg tgcggctcag acattacggt acaaaaagat tcggtgcaaa
     1141 cactctgggg cgaggtgatc tacccgcagt gcattacaca gataaactct gagcccgggg
     1201 tgggaaagac cacctttgcc tataacctgt gcgcaacggg tgcagaggga ggggagtttc
     1261 tgggcatatc gttttcaaag agcctcaagg tgctctacat agacgttgaa accccggcat
     1321 ggaaaagggc gcttaagctt acaagcatct gtgacggtaa aattcctgca aggctctatt
     1381 ttctcgattc tctagacttg cattctcagt tttcagagct tctctccctc tgtaaaacaa
     1441 gcggctatga tctgatagtg tttgatacgc agagccgcat attcaatctt gagagcgaaa
     1501 acgacaacgc cgaggcaaac cgcatgatgc tccttataag aaggcttgtg acagatgcgg
     1561 gggcagctgt ggtgcttgtg catcatacat gcaagtcgga caagaagggc gtttacagcg
     1621 gcaggggggc aagcgccata ggggctgccg tggatgtggt ggtgaacatg gaggctatgg
     1681 ataatgattc ccttaagcta aaggtagaca agaaccgtat aaacggtgac tacatgacgc
     1741 tatttataaa aaagagcggc gatgactgct ttgagccttt tgtgccgcct gctgaaactc
     1801 tgtcgggctt tgagaaattc agggtacagg agtttatact ggggctggat ggagaaaaac
     1861 ccgtatggac aacctctgac cttcagtgcg agtgcagcaa gcagggcttt tcaaggaata
     1921 cgattacaag ggcaatttcc gatcttgccc agagcggaag gataaataaa atacgccggg
     1981 gtgagtatga gataatccat aaaacccata accccaaaac caccggggct atacactaaa
     2041 ccaatgggtt tttgggtcta actcttcata ccctatcccc accattgcaa agctctccta
     2101 agaaatgaaa aagcagataa aaattctaat atcgatttag tttgttataa tctgcaaagt
     2161 tttttccatt tcggaaacat atttataaga gaggagccat gtttattatg agagcgagaa
     2221 cttgcgtatt cattttgata tgctttttgg ttatacaatc ccacggcttt gcccagcacc
     2281 attcgtctca cgaaagtgat catatggatt cagccaacag ctccacacat catcattcac
     2341 tgcggccaga tgaataccca cccgttggcg taatgggcgg acacactcat cacaagggtg
     2401 gatggatgat gtcatacagg atgatgtata tgagtatgga cgggaacaga agcggtacaa
     2461 atgatctaag cacagcggat gtgctgcagg actttatggt tactccaacg gatatggacg
     2521 tatggatgca catgtttggc ctcatgtatg cccctaccga taatcttaca gttatgggca
     2581 tgatccccta tgccagtaaa tcaatgaacc atgtaacgcg aatgggtgga agtttcagta
     2641 ctaagaccga cgggatagga gacattaaac ttagcgccct ttataaaata tttgaaaata
     2701 acggtcatca atttcacata aatgcgggca ttagctttcc aacgggagat acggatgaac
     2761 gtgatgacct tcccacaggg ccgaaccagc cgcttcctta tccaatgcag cttggctcgg
     2821 ggacgtttga tctaatgccg ggactcgcat accttgggca acaccatatg tggtcatggg
     2881 gagctcaggt actgggagtg ataaggcttg gagaaaatga tgaagattat actctgggta
     2941 atgttttgag ttcaactgta tggggatcca gaaacattaa taactggatc agcacagcta
     3001 caaggctaaa ctggaaaatc tggggaaata ttaacggggc cgatccgagg ctaaatccgg
     3061 cggttgtgcc aacagctgat cctgacagga gggcgggcaa caggcttgat ctcttattta
     3121 gtctgaattt cctgataccc aaagggcctc aattaataaa aggccagagt ctggcagtag
     3181 agtttggtct gccagtgtat cagtggcttg acggcccgca gctagagacc gactggttag
     3241 tgacgtttgg atggcggtat gattgggagt tttagtggtt atttgtttcc ggtctctagg
     3301 ccctctcata tttgcccata agctgacctt catccagtat atggccactc atcgattcca
     3361 ggagctgttc cttggtggct cccggatgtg cagggggccc agagtccagc gcatagattt
     3421 taaagaaata cctgtgcact ccgccaggag ggcagggacc tccgtatcct atggttcccc
     3481 agctgttttt gccctgaatg gcgccgtctt gtacggtttc ttctgtggct atgttttcag
     3541 gcagttcatc tgcatcggga cttaaattat agataaccca gtgtacccag gttccgcgcg
     3601 gagcatcagg atcatccatt attatcgcta ttgactctgt gccgtccggg atgttttccc
     3661 agtttaaccg gggtgagata tcattgccgt cgcaggtgta cctgaccggg atcatgctgc
     3721 cgttttcaaa agcagggctt gtgagtttaa tactcatcta tagcctcctt ttatttattt
     3781 aattttgttc tttctataat tatatagact atgaaactgc atataaatag gttcgtttaa
     3841 gaaaaaattg tattatttga ctaaatggcc aaacagccaa aaaagcctga tcctggtcta
     3901 gttgcaccgc tcacaactac aaagctctct taagaaatcg aatgtgtaaa taccggttga
     3961 gtgccggtcg ctccagtaaa accttatcgc atacttgcct atgtgttcag cgttaattgg
     4021 tcttatatcg ggcgtactta catttacggg aatactgcgt ttgaatgacg atgttttctg
     4081 acttgccttt ctaagctcat tgcagcttgc gcacgggcag tacttcctca ggtcttcata
     4141 cagatagatg ctctcatgac cgtcatccca caggatgctt agagcagtat cactgaattc
     4201 acttatctct ttgggtttca ttacagcaag tgtaacagat ttagattctg ccaagaagct
     4261 tgagcagtct gtcggggtag tctgttacta ttccttcaac acccaaatct atcagacgtt
     4321 tcatgtcctc aggttcattt atcgtccagg cgtggacttc aagattctgc tttttagcgg
     4381 tacttacaaa cctctcggtg ataacctttt tacccttgta gtgttctggc acctgaaggg
     4441 caactgccgg gcttggatta taaaagccgg caaggtaaaa ctggtttaaa aaaaagaact
     4501 tgacggcctc tgattttgtg gttgatgtgg ctaccttggg catgatgctg cgaaaatcga
     4561 gaagagcctt attgctgaat gatgctacta ttgccctgtt ttgtataccg tgtttaatga
     4621 tcaattcgct tagaggtttt atcaatgaag gttctgactg cttgatctct attattatcg
     4681 ctgagtcttt gatttcggaa ataacctccg agagtgtagg tatacgtatt ccctttcccc
     4741 tgaaaggaaa agtcttccca tcgtcatcgg tccaattgta gcctgcatca aattctttta
     4801 gttcagaaag tgtataatta ttaacattgc cggatccgtt agtggtgcgg tcgatagtgt
     4861 cgtcatgcat gattactatc tcgccgtctt ttgaactatg aatatcaagc tcgattacat
     4921 ctgctccaag ctgctgcgca tttttgaaag cagtaatggt gttttcaggt cttagcccag
     4981 ggcctcccct gtgcgctatc acaagtacgc cgtcattttt tcttatatta aaaaactgat
     5041 gcttagacat aaaaaaactc ctaaaccata aataaccaag acaagaaaca agaacaaaga
     5101 atactagaaa aagcgcaata ctgaaatttt ttttaattgt cattatttta atttaatgat
     5161 ttatatgcaa agctctaggc ttgtattgtt ttaaaattta tatataaata aatctttcta
     5221 aacattagca aacaataaat gcaattagat ttattgtata taataacaaa taactgattg
     5281 tatgcagaag gtggaataag atatgaaaat agcaatttca ggactcgatc tcgaagaagg
     5341 caaaataaag tatgaagacc cagtgtttaa ggggctttgt gataagtttg agccggcaaa
     5401 gcaggctccg tactattttg agctcaggca cgatgattat caggcagcag acgcaattgc
     5461 tattacgaaa gacagtgttc tggatctact tattctggat atagaaaagc ttgaaaaccg
     5521 catagaaagg gcggaatccg aagaggaaaa ggctgtacta aagagatgtc ttgaactgct
     5581 tgagaacgaa cagcctgtat gtgacctcga aattacagat gaagaaagat caatccttac
     5641 tactataagc ccgcttagtt tcaagcctgt tctggttctg gaagattcaa atattgatgc
     5701 aaatacagtc tgcaaacgga tgctggataa agcagggatg atgtttttct ataccgcagg
     5761 aaaacaggaa gtgcacgcat ggatagttga aaagggcacc gatgccgtaa cctgtgcagg
     5821 gaggatacat tccgatcttg caagaggatt cataaaggca gagcttattt cttttgacga
     5881 cctcatggcc gcccacagct ttgcagatgc aagctccaaa ggacttacaa acctggttga
     5941 taaagatttt cctgttcctg agaatacaat actggaaata aggtttaatg tgtgaattgg
     6001 tttatagtag taatagttct aaattcacac aaggaggtta tattgcaaaa tataaaagat
     6061 cacaaagagg tgctggacag cattgctctt ttaaaaatcc ttgctatgag ttctaagagt
     6121 ataaaagaag gtagagtaaa aacagctaag aaggcatttt ccgatataag gaaaagtatt
     6181 gaaaaagaca aaaagtcttc aagcttgtct tctaaatagt tttatcagct ttgagacatc
     6241 ttcaaaatct tatcaaactc tttctttgta acaggcatta cggatagcct gctgtttttt
     6301 ataagagcaa tgtcttttaa tgacggctca gccttgattt gttttaatgt aacgggactg
     6361 ctgagtgcac cggcaggttt tagatcaacc accacccacc tgtcgtcatc agtcgtaggg
     6421 tcctgataga attcctttat aacctctgct actcctacga cttcaagtcc ttcattgctg
     6481 tgataataca aaaccctgtc cttaatcttc attgccttaa ggttgtttct tgcctgataa
     6541 ttacgcacgc cgtcccagta agtagaaccg tcttttataa aatcgtccca tgagtactta
     6601 aaaggttctg attttactag ccagtaattc atggatttct attatatcaa taaattaaat
     6661 taataaaaat ttatatgctc aatagaatag acaggttaat cgagtatatt atgtattatg
     6721 attataaaat attgcactgt ctgcttactt attacattta tatattcgtg tgttcctgct
     6781 cctgtagggg tagatacagc gggtatagac gcaaagaata aaaccattgc catactgcct
     6841 tttggccttg aaaaagataa tgaggcgctt caagtccctg agggatactt gcagagattt
     6901 aatcacagta tgcatgagtc attatttaat tcgcttcatt ccactctggt tattgattta
     6961 aacaaaagct atgagatttt gagaaatacc gaatcttatg ataatagttt tgcagagttt
     7021 tctgctcaga gcggagcgga ttatatactc acaggttttg taagtcatta taaacaaagg
     7081 gtaggc
//
LOCUS       DBNGSV010000300         8242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1715716, whole genome shotgun sequence.
ACCESSION   DBNGSV010000300 DBNGSV010000000
VERSION     DBNGSV010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1715716"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..2134
                     /gene="acs"
                     /locus_tag="AC159U_10940"
     CDS             188..2134
                     /gene="acs"
                     /locus_tag="AC159U_10940"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR3337606.1"
                     /translation="MKNSNTFEVLLKEDRTFNPKKDFTEKANINSHSVYQKAKKDNIK
                     FWEGFANELHWFRKWRKTLMWKPPFAQWFIGGKINVSYNCIDRHLETSRKNKAAIIWE
                     GEPGDSRVLTYWDLYREVNRFSNVLKSLGVKKGDRVTIYLPMIPELAIAMLACTRIGA
                     PHSIVFGGFSAESLSGRINDSKSKILITADGGYRRGGVVPLKANSDDALKSAPSVKKV
                     VVVNRTDTKIRMKKGRDFWWHDLMEGADSSFPAQHMDSEDMLFILYTSGTTGKPKGIV
                     HTTGGYLTGVYATTKWVFDIKEEDTYWCTADIGWVTGHSYIIYGPLANGATTLMYEGT
                     PDYPHRGRFWEIVEKYGVNIFYTAPTAIRAFMKWGESHPGKFDLSSLRLLGSVGEPIN
                     PEAWMWYYNIIGNKNCPIVDTWWQTETGCILITPLPGITKLKPGSVTHAFPGIEADIY
                     DEDGKSIKRGAGNLVLKNPWPSMLRTIYGDKNRYMKTYWSKYKDVYLTGDGAKRDEDG
                     YLWVLGRLDDVINVSGHRIGTMEVESALVDHKTVVESAVIGKQHELKGQAISAFVTLK
                     EGIKCNNRLIDELKKHVVKKIGAIARPEDIIFTAELPKTRSGKIMRRLLRDIAEGRVL
                     GDTTTLADPSVVNALKDKYEHQES"
     gene            complement(2187..3596)
                     /locus_tag="AC159U_10945"
     CDS             complement(2187..3596)
                     /locus_tag="AC159U_10945"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family alpha/beta hydrolase"
                     /protein_id="HIR3337607.1"
                     /translation="MKTIRFSVFIVAILTISCGVEYNPPDLGTLYSQSAMSSDLNRNP
                     VIVIPGILGSKLLDQQTGQVVWGAFEKDYANPKDRDGAKLIAHPMKIGTPFAELKDNV
                     YPNGALDRVRVRIFGLSFLLNAYVNILSSLGVGGYVDEELGKSGFVDYGDEHFTCFQF
                     AYDWRRDNVENAKLLHNFIIEKRQYIRDEYNKRYNVDKDDIKFDIVAHSMGGLVTRYY
                     LRYGDSELPENGADLKVTWKGAYYVENVIIVGTPNAGSLDALDQLINGKKIGPFLPTY
                     TSTIIGTMPSVYQLLPRTRHKRVLDENGNAVDIMDSNTWEKYNWGLANKERISDLQKI
                     FPDDTDENLIMDTAKDHLQKSLIRAKLFHKSLDIPKAPPDTLRLYLIAADAVETPSVM
                     SVSNTTGELVIEESAPGDGTVLRASTLMDERVGGKWEARLVSPIKWSNVMFIFNDHLG
                     MTKDPAFTDNMLYILLEQS"
     gene            complement(4031..5740)
                     /locus_tag="AC159U_10950"
     CDS             complement(4031..5740)
                     /locus_tag="AC159U_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337608.1"
                     /translation="MARVVRRIQIKSLVILFLFTIISYGNEYSDILVSYSFDDDNIET
                     GPDTFKIWETGKGCVDLTDSYRFSGYNSVQISDGVGDKDFPELMGFFPTMKTGKLYIH
                     FSFLTTDHNDFLNIALAGPKFFNLTKDGIGFWLKTQKGYLYHVSDSMPKKLFAIHAFR
                     WYSVDIEYDIDKGTYDLKITEENHYTPLIELKNQKNASNNPKSVVNVFSFVTDPFTDK
                     SNLTYYIDDVIIGLDEEILKTSFVAPGRKKYFVDMWDDYQNKLRDKPGCLPAIHPYDY
                     GLNDNKINELGKQGLLGLINKAASGEQIPLDKTKDISLQNLDILNAVYKWSRGCSLLA
                     SNKPSKALELFNSSAQTLQYAPVVELSRILALARLNRWGEVNDGLRNLYFEGVDDQRI
                     SVVQAMLGMLNTDLQNAEKWLEEPAQTIPDKFGDKISNNIIRRFWSGKIDKDLFIAAK
                     KYMPYDWLGYYQQAHIAEQYFYVLLWQEKNEEAAIFADRMIERLELLAVPTSIWLERR
                     GDAAFYMKDYNTAEKFYQKALVKKRKLKLIELCMKSYQMFITSQVTMIKKGYIEKKFM
                     EAL"
     gene            complement(5701..6345)
                     /locus_tag="AC159U_10955"
     CDS             complement(5701..6345)
                     /locus_tag="AC159U_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3337609.1"
                     /translation="MSRVHTVLFILLFFIGINVCTRAQQIDKDEVIKYVSSYNVSALK
                     SLGPGVMPILSNLYIVSDSKQKRVIANIFYQLGFLSGNAKSVLMQDVRTNDKQLRVAV
                     QYALGRVSSDEDVVDALLYNMHNDSNAHFRDKAACALAYDQIHLTENEKVDLYEGLIK
                     GLSSPNRQIRSIAIKALKIHTGQTKGFLVNGTASNQSESLRKWHAWLEEYKSNL"
     gene            complement(6388..7980)
                     /gene="nadB"
                     /locus_tag="AC159U_10960"
     CDS             complement(6388..7980)
                     /gene="nadB"
                     /locus_tag="AC159U_10960"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR3337610.1"
                     /translation="MSQQSTNVHSDFLVIGSGLAGLYSAYYASKFGSVSLVTKSTLEQ
                     SNSYWAQGGIAAAIDPDDSHMYHKEDTLKAGRGLCNTEAVDILVREGIQRVLSLMKLG
                     MKFDVGDEGFHLGMEGGHSKRRVLQSGGTSTGKEIVSFLINEIKKSEKINVFENTQVL
                     KILTQSGRCQGGIAVNYYDNNTYIFSSNATILATGGASALYQRSTNPPGATGEGIALA
                     YNEGARVMDMEFIQFHPTSYYSESGKSFLLSEALRGEGAILLNESGERFMESVHERAE
                     LAPRDVVASAIFREIRKSEKPFVFLSVKHLDSGLIKEKFTNIYEFCLSENLDITAEDI
                     PVAPAAHYFIGGIKTGLMGETNIDGLYACGEVASNGVHGANRLASNSLLECLVFSKRA
                     IDHAKDSFNSRTSKSPEIDLNRYKYQTSQEQQTIYLDIKNKVSQIMNELVGIIRKEEE
                     LHEALKQLEAHGELVCKLSSFYSIQLQNIITVCLLITKSALCRQESRGAHIREKYPEE
                     NEDWNTHIVWEKKNSQPLIEKI"
     gene            complement(8001..>8242)
                     /locus_tag="AC159U_10965"
     CDS             complement(8001..>8242)
                     /locus_tag="AC159U_10965"
                     /inference="COORDINATES: protein motif:HMM:NF051340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337611.1"
                     /translation="NESSDDFTIIEYYSNDRIGLLYEVSRELSNLGYSIEYAKISTKV
                     DQVSDVFYITDSNNKKILSSKKLREIKDTLLSLDS"
BASE COUNT         2465 a         1558 c         1515 g         2704 t
ORIGIN      
        1 agctgttata tcagataata aatccaaaaa ctatgagcaa acatgtactt caactcaatg
       61 acaaaatatc agcaaattct aactctaaag gtggacctga attctggggc ttgacagtaa
      121 gatttaaaat aatatattct tattggtgtt tatcaaaatt ttaaatattc caaatttaat
      181 gtgaataatg aaaaactcaa acacttttga agtgcttctt aaggaagaca ggacttttaa
      241 tcctaagaaa gattttacag aaaaggctaa tataaacagc cactctgtct atcaaaaagc
      301 aaaaaaagat aacataaaat tctgggaagg ttttgcaaat gagcttcatt ggtttagaaa
      361 gtggagaaaa acactaatgt ggaagccgcc ctttgcacag tggtttatag ggggcaaaat
      421 aaatgttagc tataactgta ttgacagaca cttagaaact tcgagaaaaa ataaggcagc
      481 aatcatctgg gaaggggagc ctggtgattc aagagtacta acatattggg atctgtatag
      541 agaagttaac agattctcta atgtacttaa gagtcttgga gttaaaaaag gggacagagt
      601 tacaatctat ctgcccatga tacccgagct tgccattgca atgcttgcgt gcacaagaat
      661 aggggcacca cacagcatag tttttggtgg ttttagtgct gagagcctta gcggaagaat
      721 aaatgacagt aaatcaaaaa ttctaattac agcagatggt ggatacagaa ggggcggagt
      781 ggtgccactc aaggcaaact ctgatgatgc tcttaagtct gcaccgtctg ttaaaaaggt
      841 cgttgtggta aatagaactg atacaaaaat cagaatgaaa aaaggccgtg atttttggtg
      901 gcatgatctt atggaaggtg cagacagttc gtttcctgcc caacatatgg acagcgaaga
      961 catgcttttc attctctaca caagcggaac tacaggcaaa ccaaaaggaa ttgtgcatac
     1021 aactggaggt tatttaaccg gagtatatgc aacaactaaa tgggtgtttg atataaagga
     1081 agaagataca tactggtgta cggcagacat tggatgggtc accggacata gttatataat
     1141 ctatggtcct cttgcaaacg gggctacaac cttaatgtac gaaggtacgc cggattaccc
     1201 gcacagggga aggttttggg agattgttga aaaatatggg gtaaatatct tctatactgc
     1261 acctactgcg ataagggcat ttatgaaatg gggtgaatct caccctggca aatttgacct
     1321 cagttctttg agacttcttg gcagtgtggg agagccgatt aatccagagg catggatgtg
     1381 gtattacaat atcattggta acaagaactg tcctatagtt gatacatggt ggcagactga
     1441 gactggatgt atcttaatta ctccactgcc tggaataaca aaattaaaac caggctcagt
     1501 tacccatgcc tttcccggaa tagaagccga tatttatgat gaagacggga agagtatcaa
     1561 aagaggagcg ggtaatttgg tattaaaaaa tccttggccg tctatgctta gaaccatata
     1621 tggtgataag aatcgttata tgaagaccta ttggagcaag tataaagatg tttatcttac
     1681 aggagacggc gccaagaggg atgaggatgg atatctatgg gtgcttggaa ggcttgatga
     1741 tgttataaat gtatcagggc acagaatcgg cactatggag gtggaaagtg cgcttgttga
     1801 tcataaaacc gtcgtagaat ctgctgtaat aggaaaacag cacgagctta agggccaggc
     1861 aatctcagca tttgttactc tgaaagaagg tattaaatgt aataacaggc ttattgatga
     1921 actaaaaaaa catgtagtca aaaagatagg tgccattgca agacccgaag acattatttt
     1981 cactgcagaa ttaccaaaaa caagaagtgg taagataatg agaagacttc taagggatat
     2041 tgccgaagga agggtgcttg gagatactac aacgcttgcc gacccctctg tggtaaatgc
     2101 ccttaaagat aaatatgagc atcaggagag ctaataactg gatttaatga caaacttcac
     2161 ttttgatttt attttagtgt gttctattat gactgttcca aaagaatgta cagcatattg
     2221 tcagtaaaag cggggtcttt tgtcattccc agatgatcgt tgaatataaa catcacattt
     2281 gaccacttta ttggggatac gagccttgct tcccacttcc cgccaactct ttcatccatt
     2341 agtgtacttg ctctaagtac ggtgccgtca ccaggggctg actcttctat tactaactct
     2401 ccggttgtgt ttgaaacgct cattaccgaa ggggtttcta ctgcatctgc tgctatgaga
     2461 taaagtctca gagtatccgg aggggctttt ggtatatcca gagatttatg aaaaagctta
     2521 gcccttataa gagatttttg taagtggtcc ttagctgtgt ccattatgag gttctcatca
     2581 gtatcatcag gaaaaatctt ttgcagatca gatattctct ctttatttgc aaggccccag
     2641 ttgtattttt cccacgtatt ggaatccatt atatctactg catttccatt ttcatctaat
     2701 actcttttgt gtcttgttct aggaagaagt tggtaaaccg agggcattgt gccaattatt
     2761 gtcgatgtgt atgttgggag gaacgggcct attttttttc cattaatgag ctgatccagg
     2821 gcatctaaag atcctgcgtt gggagtaccg acaattatta cattttcaac ataatatgca
     2881 cccttccagg taactttaag atcggctcca ttttcaggaa gctcagaatc tccatatctg
     2941 agataatatc ttgtaacaag accccccata gaatgagcta caatatcaaa ctttatatca
     3001 tctttatcaa cattatatct tttattatat tcatccctga tatattgtct tttttctatt
     3061 ataaaattat gaagcagttt ggcattttcc acattatctc ttctccagtc ataggcaaac
     3121 tgaaagcatg tgaaatgttc atcaccatag tctacaaaac cagatttacc cagctcttcg
     3181 tctacatagc cgccaacccc aagcgagctt aaaatgttta cataagcatt tagcaaaaat
     3241 gacaggccaa aaattcttac ccttactctg tcgagtgcac cgtttgggta aacattatcc
     3301 ttaagttcag caaatggcgt tcctattttc atgggatgcg caatgagctt tgccccatct
     3361 ctgtctttag gatttgcata atccttttca aatgcacccc aaaccacttg acctgtttgc
     3421 tgatctaata gcttcgagcc aagaataccc ggaataacaa ttaccggatt tctgtttaaa
     3481 tcggatgaca ttgccgattg tgagtacaat gtacccaaat caggagggtt atattccacc
     3541 ccacaggata ttgtgagtat agctacaata aatactgaaa acctgattgt tttcatatct
     3601 tagttttatt gttaagtaaa taatgatgct ctatctgtat ttgaatgatt atcttatatt
     3661 acagattcaa aatcaattat attatttagt tatataaatt agaatttaaa agttacttat
     3721 aacaacaatt tttggtagag gcttaaggtt ttgggaagtt ttcaaaatcc tcaatcttta
     3781 taaatagcta gtaacataga tattgaaact aattagcgac tggcacaatt tttgcttaat
     3841 atttaatgct atgaatttgt tattacaaag actgaaacag aaaagaatta agatatttaa
     3901 gactaggtta tcactagaag atatactgtt tatatctata ttaactagtt taatctttgc
     3961 ggttgcattt attggataat aataatcaag attaatatta tattgatttg aaactatcta
     4021 cattttttat ctacaaggct tccataaatt ttttctctat ataacctttc ttgatcatag
     4081 ttacctgtga ggtaataaac atctgatagc ttttcataca aagctctatc agttttaatt
     4141 tcctcttttt cactaaggct ttttgataaa acttttcggc tgtattgtag tctttcatat
     4201 aaaaagcagc atcacctctt ctttcaagcc atatagacgt gggcactgca agtaattcca
     4261 ggcgttcaat catgcggtca gcaaatattg cagcctcctc atttttttcc tgccataaga
     4321 gtacataaaa atactgttcc gctatatgtg cctgctgata atagccaagc cagtcataag
     4381 gcatatattt ctttgctgca atgaataaat ctttatctat ttttcccgac cagaagcgtc
     4441 tgattatatt gttggagatc ttgtcaccaa atttatccgg aatggtttgg gctggttctt
     4501 ctagccactt ttctgcattt tgaagatctg tatttaacat ccctaacatt gcctgaacta
     4561 cggatattct ttgatcatct actccttcaa aatacaggtt tctaagtccg tcatttacct
     4621 caccccatct gtttagtctg gcaagtgcca atatcctgga tagttcaaca accggagcat
     4681 actgcagtgt ttgcgccgac gaattaaaca gttccagagc ttttgacggc ttgttcgatg
     4741 caagcagcga acatccccgg ctccatttat acactgcgtt taatatatct aagttttgga
     4801 gtgaaatatc ttttgtttta tctaaaggaa tttgttctcc acttgctgcc ttgttaatta
     4861 aaccaagaag accctgtttt ccaagctcat taattttatt atcgtttagc ccgtaatcat
     4921 acgggtgaat tgctggaagg cagccgggtt tatctcttaa cttattctga taatcatccc
     4981 acatatcaac aaaatatttt tttcttcccg gagcaacaaa actggttttt aatatctctt
     5041 catccagccc tattattacg tcgtcaatgt aataggttag attcgattta tctgtgaatg
     5101 gatctgttac aaaagagaaa acattaacta ctgattttgg attgtttgat gcattttttt
     5161 gatttttaag ctctataagc ggcgtatagt gattttcttc ggtgattttg aggtcgtatg
     5221 ttcctttgtc tatatcatat tcaatatcaa ccgagtacca tctgaaggca tggatagcaa
     5281 agagcttttt aggcatgctg tctgatacat gatatagata acccttttga gttttaagcc
     5341 aaaatcctat gccgtctttt gttaaattaa aaaacttggg acctgcaaga gcaatattga
     5401 gaaaatcgtt atgatctgtt gtaagaaaac taaagtgtat atataacttt ccggttttca
     5461 ttgtagggaa gaaccccata agttcaggaa agtccttgtc tcctaccccg tcactaattt
     5521 gaaccgaatt atatccgcta aatcgatatg aatctgtaag gtctacacag cccttgcctg
     5581 tttcccatat tttaaaagtg tcaggccctg tttctatatt gtcatcatcg aagctgtagc
     5641 ttactagaat atcagaatat tcgtttccgt aagatattat ggtaaaaaga aataatatta
     5701 ctaaagattt gatttgtatt cttctaacca cgcgtgccat tttctcaaag attcggattg
     5761 attacttgca gtaccattta ccaaaaatcc ttttgtctgt ccagtgtgta tcttaagtgc
     5821 ttttatggca atactgcgaa tctgtctgtt tggacttgaa agtcctttta tcaaaccttc
     5881 atacaaatca actttttcat tctctgttaa atgtatctgg tcataagcaa gggcacatgc
     5941 tgccttatct ctaaagtgtg cattagagtc attatgcata ttatataaaa gcgcatctac
     6001 aacatcttca tcactgctca ctcttccgag cgcgtactga acagccactc taagttgttt
     6061 gtcgttggtt cttacgtcct gcatgagcac gctcttagca ttgccggata gaaacccgag
     6121 ctgataaaat atattggcaa taactctttt ttgttttgaa tcggaaacta tatacaggtt
     6181 tgaaagtatc ggcataaccc ctggccctag agattttaga gccgatacgt tataggatga
     6241 aacatattta atgacttcat ctttgtctat ttgctgggcc ctggtgcaaa catttattcc
     6301 aatgaaaaat aatagaataa ataaaactgt atgtactcta ctcataacaa tattataata
     6361 cgaaaataag taatttgggt ttttttatta aattttttca attaatggct gagagttttt
     6421 cttttcccaa actatatgag tattccaatc ttcgttttct tcaggatatt tttctcttat
     6481 atgtgcccct ctggattcct gtctgcaaag cgcagattta gtaatcagca ggcatactgt
     6541 gattatattt tgtagctgga tggaataaaa actactcagt ttacatacaa gttctccgtg
     6601 tgcttccaat tgcttaagtg cctcatgaag ctcttcttct tttctaataa ttcctactag
     6661 ttcattcata atttgagata ccttgttctt tatatcaaga tagatggtct gctgttcttg
     6721 actggtttga tacttatacc tatttaaatc aatttctggt gacttagaag ttctgctatt
     6781 aaacgaatct tttgcgtggt cgatggctct ttttgaaaac acgaggcatt ccaataggga
     6841 gttgctcgct agtctgtttg ctccatgcac tccgtttgat gctacttcac cgcaggcata
     6901 taacccgtca atattggtct ctcccataag tcccgtttta attcctccta taaaataatg
     6961 ggcagcggga gcaaccggta tgtcctcagc tgttatatca agattttcac ttaggcagaa
     7021 ttcataaata tttgtaaatt tttcttttat aaggccgctg tccagatgtt ttactgatag
     7081 aaagacaaaa ggtttttctg attttcttat ttctctaaat attgcacttg cgactacatc
     7141 tcttggcgca agttcagcac gctcgtgcac tgattccata aacctctctc cactttcgtt
     7201 taaaagtatt gctccctcac ccctaagcgc ttcactcaat aaaaagcttt ttccagactc
     7261 tgaataataa gaagtcggat ggaactgaat aaattccata tccattaccc gggcaccttc
     7321 gttatacgca agtgctattc cttcacctgt agcacccgga gggtttgttg atctttgata
     7381 aagcgctgaa gcaccgcctg tggccaaaat agtagcgttt gaactaaaaa tgtaggtatt
     7441 attatcatag taattaactg ctatcccacc ctggcatctg cctgactgcg ttaatatttt
     7501 aagtacctga gtgttttcaa aaacattaat tttttcactc ttttttattt cattaattaa
     7561 aaagcttacg atttcttttc ctgtggatgt accaccagac tgaagtaccc ttctttttga
     7621 atgccctcct tccataccca gatgaaatcc ctcgtcaccg acatcgaatt tcatacctag
     7681 tttcataagg cttaagactc gctgtattcc ttctcttacc aaaatatcta cagcttcagt
     7741 gttgcaaaga cccctgcctg cttttaaagt gtcttctttg tgatacatat gggaatcatc
     7801 ggggtcaatc gctgctgcaa tacccccttg cgcccaataa ctgttgcttt gctcaagtgt
     7861 tgatttggta acaagtgata cagagccgaa ttttgacgca taatatgcag agtatagtcc
     7921 tgcaagccca ctgcctataa ctaagaaatc tgaatggaca ttggttgatt gctggctcat
     7981 atgttaggga taaacaccat ttaagagtcg agagatagaa gagtatcttt aatctctctt
     8041 aattttttag aagataaaat tttcttattg tttgaatctg taatataaaa aacatctgat
     8101 acctggtcta ctttagtaga tatttttgca tactctatgg aatatcccag gttgcttagt
     8161 tctcttgaga cttcataaag aagtcctatt ctgtcattgg aataatattc tattattgta
     8221 aagtcatcag agctttcatt gt
//
LOCUS       DBNGSV010000301         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1368505, whole genome shotgun sequence.
ACCESSION   DBNGSV010000301 DBNGSV010000000
VERSION     DBNGSV010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1368505"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1037
                     /locus_tag="AC159U_10970"
     CDS             285..1037
                     /locus_tag="AC159U_10970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3337612.1"
                     /translation="MKICTLASGSSGNSLYVETEHTKILVDAGISNKQITKRLSSIDV
                     DINDIDAVVLSHEHTDHALALPYLKVPVYVSSSIAYIWSDKVKNLRLFETGSTFSVND
                     TTIRSFPVPHDAVDPVGFTLQADKHKLGVVTDIGVTTRLISERLKDCNLLVVEFNHDE
                     NMMLYSPYPWHLKQRIKSRLGHLSNTEASSLLSTIAGMDLHSVVLAHLSKVNNKADSA
                     LEFAYNALKKLGLGDVNINIAPRNNISEVIEI"
     gene            1034..2326
                     /gene="purB"
                     /locus_tag="AC159U_10975"
     CDS             1034..2326
                     /gene="purB"
                     /locus_tag="AC159U_10975"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3337613.1"
                     /translation="MISRYSREEISKIWTDESKYSYMLKVELAACDAWAKLGKIPKRS
                     LQTIKKKADFKVSRINQLEKTLKHDVIAFLTSVAEYVGPDSRFIHMGLTSSDVLDTAF
                     ALQLRDSSDIILKDIKEVLTAIKKLAKKHKYTPMVGRTHGIHAEPTTFGLVAALWYDE
                     MSRNLERMKRAKEAISVGQISGAVGAYVNIPPSIEKDACRILKLKPANISTQVIQRDL
                     HAEFFLTLSMIASTIEKIALQIRHFQRTEVLEAEEGFTKGQKGSSAMPHKKNPILSEN
                     LCGLSRLVRSNGSAALENNPLWHERDISHSSVERVIGPDSTILTDFMLARVKSLIDNL
                     VVYPNNMKRNMDITNGLIHSETVLLKLVDKGITREKAYTLVQKNAMKCWEQKTPYKDL
                     LIADPDVMKYLTEKEIESCFDLKGEFKNIDMIFKRVFN"
BASE COUNT          890 a          395 c          483 g          698 t
ORIGIN      
        1 catatggaac ccagttctct tcggttttgt caaagtcagg aatttcaatt aattcataag
       61 aataattaat gttactgtca atatttttat aacttattat cttaatagga ctacaaccag
      121 taaaaattat tccggtaaat ataaagataa tgataaataa gttcctagct aacataacac
      181 ctcctttttt agaataataa atacaatact taattaaaaa aaaaataaaa taaaataaca
      241 gattattatt gatattataa caaataaaat attaattttg acagatgaaa atttgcacac
      301 tagcaagcgg aagctcagga aattctctat acgttgagac tgaacataca aaaatattgg
      361 ttgatgcggg tatcagtaat aaacaaatta ccaaaaggct tagcagtatt gatgttgata
      421 ttaatgacat agacgctgta gtgttaagcc acgaacacac tgaccatgct ctggcacttc
      481 cgtatttaaa ggttccggtt tatgtatcaa gttcaattgc gtatatctgg agtgataagg
      541 taaaaaacct aaggctgttt gaaactggaa gcacttttag tgtaaatgat acaacaatta
      601 gatcatttcc cgtaccgcat gatgcagtgg atccggttgg ctttactctg caagctgata
      661 agcataagct tggtgtagta acagatattg gtgtgaccac caggcttata agtgagaggt
      721 taaaagattg caatttgctt gttgttgaat ttaatcatga tgaaaacatg atgctttaca
      781 gcccttatcc ctggcatctt aaacaaagaa taaagagcag gcttggacat ctatcaaata
      841 cagaggcctc cagtttattg agtaccattg cgggcatgga cttacattca gtggtactgg
      901 cacacctgag taaagtgaat aataaagcag attcagctct tgagtttgca tacaatgcac
      961 tcaaaaagct gggtctgggg gatgtaaata ttaacattgc accaagaaat aatataagcg
     1021 aggtaataga aatttgataa gcagatacag tagagaagaa atatcaaaaa tttggacaga
     1081 tgagtcaaaa tattcatata tgttaaaagt agagcttgct gcatgtgatg cttgggcaaa
     1141 gctaggcaag attcctaaaa ggtcacttca aaccataaag aaaaaagcag atttcaaggt
     1201 ttcacgaatt aatcaattag aaaagactct aaagcatgat gtaattgcat tcttaacttc
     1261 agttgccgag tatgtagggc cggattccag atttatacat atggggctta cttcatccga
     1321 tgtactggac acagcatttg ccttacagct tagagacagc tcagacatta ttttaaaaga
     1381 cataaaagaa gttcttacag caataaaaaa acttgcaaaa aagcataaat ataccccaat
     1441 ggtaggaagg actcatggta ttcatgcaga gccaacgact tttggtctgg tggcagcgct
     1501 ttggtatgac gagatgagca gaaacctgga aaggatgaaa cgggcaaaag aagctataag
     1561 cgtagggcag atatccggag cagtcggggc ttatgtaaat attccgccat ctatagaaaa
     1621 agatgcttgc agaattctta aacttaagcc tgctaatatt tctactcagg ttatacaaag
     1681 ggatttacat gcagaatttt ttcttaccct ttctatgatt gcttcaacaa ttgaaaagat
     1741 tgcccttcag ataagacatt ttcaaagaac ggaagtttta gaggcagaag aaggatttac
     1801 aaaaggacag aaagggtctt cggcaatgcc ccacaaaaaa aaccctatac tatcagaaaa
     1861 cctgtgtggt ctctcaaggt tagtaagatc aaatggctca gctgctcttg aaaataaccc
     1921 tttatggcac gaaagagata ttagtcattc ctcggtagaa agggtaatag ggccggacag
     1981 cactatactt actgatttca tgctagccag ggtaaaatca ttaattgata atcttgtggt
     2041 ttatccaaat aatatgaaaa gaaatatgga tattactaat ggccttatac actctgaaac
     2101 ggtgctactg aagcttgttg ataaaggaat tacaagagaa aaggcttaca cacttgtgca
     2161 aaaaaatgca atgaaatgct gggagcaaaa gaccccctat aaagacctgt taattgcaga
     2221 tcctgatgta atgaaatatc tgactgaaaa agaaattgag agctgttttg atctcaaagg
     2281 tgaattcaag aatatagata tgatttttaa acgagtattt aactaatact gatagtatta
     2341 ttttagataa aaataaaaaa ccccggcaag cataatgttt accggggttt tctggctttt
     2401 atgggtcagg agagatgagc aaatgacctt aatataagcc tgcgtgtttt tagaaataag
     2461 cttgta
//
LOCUS       DBNGSV010000302         8128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_608161, whole genome shotgun sequence.
ACCESSION   DBNGSV010000302 DBNGSV010000000
VERSION     DBNGSV010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_608161"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC159U_10980"
     CDS             complement(<1..257)
                     /locus_tag="AC159U_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3337614.1"
                     /translation="MMELTTDIEVLKEHARKIRIDLLTALNNCNSGHTGGSLSCTDIL
                     ATLYFSVMRYDPKNPGWQDRDRFIMSKGHAAPALYATLAEAG"
     gene            703..1431
                     /locus_tag="AC159U_10985"
     CDS             703..1431
                     /locus_tag="AC159U_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3337615.1"
                     /translation="MDTSNLPNHVVIIMDGNGRWAQKNNLDRISGHREGMKSVKSVIQ
                     ESRKIGIKYLTLYAFSAQNWNRPRPEVNALMELLTFYLLEQGQALLEKDIRLNAIGRL
                     NSLPEDARNVLNQAMELTKDCCAMTLTLALSYGGREEIIDAVKKICDKPNLSSKDINE
                     DCFSNYLYTSDMPDPDLLIRTSGEHRISNLLLWQLAYTEIYFTKTLWPDFRKRHLVKA
                     ISNYQNRERRFGLTAEQLKTREVI"
     gene            1428..2579
                     /locus_tag="AC159U_10990"
     CDS             1428..2579
                     /locus_tag="AC159U_10990"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3337616.1"
                     /translation="MKNISILGSTGSIGTQTLEVISGFPQKFNIVGLAGGNNFDLLAE
                     QVIKYRPKIVSIKSEANAAALESKLKNIKTHIVYAEKGAEQVASHPEADLVVSSMVGA
                     SGLKPTLAAIKQGKDIALANKETLVMAGQLITDEAKKNNIKIIPVDSEHSAVYQAIKT
                     SDKTYVKRIILTASGGPFLNTPVNEMKDISVSQALNHPTWKMGNKITIDSATLMNKCF
                     EIIEAKWLFDLSNEMIDVWVHPQSIIHSMVEFVDGSVMSQMGLPDMKIPISYSLNYPE
                     RLNLNINSVQPGEFSNLQFEEVDTTKFPSINYAFHALKEGGTLPAVMNAANEIAVMEF
                     LEGNIQFTQILKTIKDVMDLHNNSQDYNYEAIMESDKWARNKTREILSN"
     gene            2589..4184
                     /gene="rseP"
                     /locus_tag="AC159U_10995"
     CDS             2589..4184
                     /gene="rseP"
                     /locus_tag="AC159U_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3337617.1"
                     /translation="MSTVLGFLFVIGVLVFIHELGHFLVAKWSGVRVEKFSLGFGKKL
                     FGFRRGETEYQVSMLPLGGFVKMYGETGEGCIIVEDIKEGSKAEKLGLISGDKIMKID
                     GNELKEFSTWKSLLHRLKTSDKTEHEYLVERDDDRIRLNGSLEDIDGMNAFSEKEYSR
                     GFSNQPLLNRFLIVIAGPAMNFLVPFVFFPMVFLLGIEQPAYLDKVPEVGYIQPESEA
                     QKAGFEIGDRILKINNKPVNTWKEATIAFQINPDSLLSVTVDRAGTEKELSVKAIAGN
                     QGVVSIGVLEPLDAVVGNVGPGTPAKDAGLQKGDKIIQIDDVKINDWYQMSAAIKKRG
                     DKESTFIILRDDKELSIQITPALKENDQGQIGIGPLQEQTVQKYGLFKSITKGMLKAY
                     HDIIEITELLFGFLYKLITGAIPLSTAGKTIAGPLLIAQVSGAAAESGWATLLQFTSF
                     ISINLAIINLFPIPMLDGGHVVYIGLEAVRRRPLSEKAMEYSQKVGFTFLILLMFLAV
                     FNDVSRLKNDIIKPFKKAAEYIK"
     gene            4205..5110
                     /locus_tag="AC159U_11000"
     CDS             4205..5110
                     /locus_tag="AC159U_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3337618.1"
                     /translation="MSLLKLGKDNWARTSPTKLGILYIALTLFVGFAAINTGNNLLYL
                     TFGVMLSFVIVSGIISMFNLARIDVDFKIPRDVYALSSTNLVFSLRNNKPLIPSYSLT
                     IDLNGRKAFVPYLKRDAKLKTNLLYVFSKRGWNTIPDATISTSFPFGFFKKWIRIDLG
                     KKEVLVYPKIEKIDISDSFIQEKKGENEIDKKGFGADLRSIKDFSQGDNPKFIHWKVS
                     AKKNKLMFKEMQDEESESVTLQFNPTKNEGELERQIVQIASTLMELLRKNYLVDFISP
                     QKTYPHSQIGNPPREVLSYLALYNN"
     gene            complement(5112..6701)
                     /locus_tag="AC159U_11005"
     CDS             complement(5112..6701)
                     /locus_tag="AC159U_11005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3337619.1"
                     /translation="MSELEKFENLGVSQEMLAALRKKGFEEPTPIQVKIIPILLSANK
                     DLVGQAQTGTGKTAAFGIPILENIEENTKYVQVIILTPTRELAIQVSEEINSLKGRKK
                     IQVIPIYGGQSMELQLKRLKKGVEVVVGTPGRVLDHIKRGTLKLENVSYAVLDEADEM
                     LNMGFIDDVEEILAATNKERTTLLFSATMPREIMAVAKRHMKGYELIKTETEKITVDL
                     TDQIYFEVPGRDKFEALCRIIDFEKEFYGLIFCRTKIDVDTVANHLKDRGYDTEGLHG
                     DISQHRREKILTSFKDKRINILIATDVAARGIDIDNLSHVINYSIPQDPESYVHRIGR
                     TGRAGKSGTAITFITPEEYRKLMHIKKAAKTDIRRERLPKVDDIIEVKKDRIKDRIND
                     IYNANIQQEFSDLAREMIGETNPEQLIASLLQTTFSDDLNPQRYNEVRDVYPKIQGKT
                     RLFIARGQKDDMNKRKLVSFIKNKANVEDRSIDDVQVFEKFSFITVPLKEAEHIIECF
                     KKDNIGRRPLVEMATKSKKNK"
     gene            complement(6798..7271)
                     /locus_tag="AC159U_11010"
     CDS             complement(6798..7271)
                     /locus_tag="AC159U_11010"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR3337620.1"
                     /translation="MPIEIERKFLIDESIVADLKEGEVIKQGYFPTTNNTAIRVRIKG
                     GKGYITIKGSRSGITRTEYEYEIPYNEAEEIIDTLCNNMVIDKTRYKIEHFDHTWEID
                     VFEGGNKGLIVAEVEMSDENEKVVLPSWIKDEVTNDIRYSNSNLVKYPYNCWEKK"
     gene            7296..>8128
                     /locus_tag="AC159U_11015"
     CDS             7296..>8128
                     /locus_tag="AC159U_11015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3337621.1"
                     /translation="MIDTNKILIIGCGVSGLTCGVKLLDSGFDVEIIASKLPADTTSS
                     VAPAYWYPYKVYPEEKVLKWGEISYREYATLSNDKNTGVTFKKLFKLFEKKVPRPYWT
                     KIVKSFNEVQKSELPGGYNYGFTVEVPQVEAPIYLKYMYNRFIELGGLVTQIEELTSL
                     EHLYNDNRIIVNCTGLGSYKLCSDSEMFPIRGQLVRTSNPGVDKIYNDEDGPLAITYI
                     VPRSDDCVLGGTAQENNWNLEVDKSISDAILSKSATLVPELRDADILEHKVGLRPGRT
                     EV"
BASE COUNT         2582 a         1551 c         1558 g         2437 t
ORIGIN      
        1 ccagcctcag caagtgttgc ataaagagca ggagccgcat gacctttaga catgataaaa
       61 cgatctctgt cctgccatcc cgggttttta ggatcatatc tcataacaga gaaataaaga
      121 gtggccaata tatccgtgca tgaaagcgat ccgcccgtgt gacccgaatt acaattattt
      181 agagcagtta gcaggtcaat acgaattttt cttgcatgtt cctttaatac ttcaatatca
      241 gttgtaagtt ccatcatata ttaaaaaatt caaagtctag cttgaaaaat gagcgataaa
      301 tataaacctc aatccggata tgtcaacaat accccggtgc actatataag aagctttaca
      361 aggtattaat aaattatagc ttgaacaggg taataaaatc agctaactta cccattctag
      421 ttatgatcgg actcataaaa gaacttagca ccagatattt tggttcgtat atggaattct
      481 ttgaaaagtt ttccaagata agcctactgc tcaaaatact tgtgattcct gtttttactt
      541 ttgtatatat actaacattt ttcacacttt tactcatagt tctaaccatt atatttgtag
      601 tattaccaat atctattgtc tacaaacctc atttggttaa gaattcttaa tattgagacc
      661 taataattag cttaaatttc caaggagttt gatataaaca aaatagatac aagcaacctt
      721 cccaatcacg ttgtaataat tatggacgga aatggaaggt gggcacaaaa aaataatctg
      781 gatagaataa gcggtcacag agagggtatg aagtctgtaa aatcagttat ccaggaatcg
      841 agaaaaattg ggattaaata tcttacgctc tatgcattct ccgctcaaaa ctggaacagg
      901 ccaagacctg aagtaaatgc gcttatggaa cttcttactt tttatctgtt agaacaggga
      961 caggctcttt tagaaaagga tataagacta aatgcgatag gaagattaaa ttcacttcct
     1021 gaagatgccc gcaatgtttt aaatcaggca atggagctaa caaaagactg ctgcgctatg
     1081 acccttactc ttgctctcag ctacggtgga agagaagaaa taattgatgc ggttaaaaaa
     1141 atatgtgata aacccaacct gagctcaaaa gatataaatg aagattgctt ttctaattat
     1201 ctttacacat ccgatatgcc tgatcctgac ctgttaataa ggaccagcgg agagcacaga
     1261 atctctaacc tccttctctg gcagcttgct tatacagaaa tttatttcac taaaaccctc
     1321 tggccagatt ttcgtaaaag acatttagtt aaagctatct ctaactatca aaacagagag
     1381 agaaggtttg gtcttactgc agaacaactt aaaacaaggg aagttatttg aaaaacatct
     1441 caatcctggg atctactgga tcaataggca ctcagacact ggaagtcatc tccgggtttc
     1501 ctcaaaaatt caatattgtt ggacttgctg gaggaaacaa ctttgatctg cttgctgagc
     1561 aggtaattaa atacagacca aagattgttt ctattaaatc agaagctaat gccgcggcgc
     1621 ttgagtcaaa actcaagaat ataaaaacgc atattgtata tgcagagaaa ggagcagagc
     1681 aggttgcctc acaccctgaa gccgatctgg ttgtttcttc aatggttggc gcctcgggac
     1741 ttaagccaac tttagcagca attaaacaag gaaaggacat tgcccttgct aataaagaga
     1801 cgcttgtgat ggctggacag ctcattactg atgaagctaa gaagaataat ataaagataa
     1861 taccggtgga cagtgaacac agtgctgttt accaggccat aaaaacctca gacaaaactt
     1921 atgtaaaaag aataattctt acagcatcag gaggcccttt tctaaacact cccgtaaatg
     1981 aaatgaaaga catatccgta tcacaggcac taaatcatcc aacctggaaa atgggcaaca
     2041 aaatcacaat tgattctgct acacttatga ataaatgttt tgaaataatt gaagcaaaat
     2101 ggctctttga tttaagtaat gagatgattg atgtatgggt gcatcctcag agcataatac
     2161 actctatggt agagtttgtt gacggctctg taatgtcaca aatgggcctg cctgatatga
     2221 aaattccaat atcctattca ctcaactatc cggagagact taacctcaat attaacagcg
     2281 tacagccagg cgaattttcc aacctgcagt ttgaagaggt tgacacaacc aaattcccgt
     2341 ctattaatta tgcattccat gcccttaaag aaggtggtac actaccggcg gtgatgaatg
     2401 cggcaaatga aattgccgta atggagtttt tagagggaaa tatacaattt actcagatac
     2461 taaagactat aaaagatgta atggatcttc ataataattc ccaggattat aactacgagg
     2521 caataatgga gtcagacaaa tgggcaagaa ataagacccg tgagatttta agtaattagg
     2581 agaaaaatat gagcacagtt ttaggatttt tatttgttat tggagtactt gtattcatac
     2641 atgagcttgg acattttctg gtagcaaaat ggagtggtgt tagggttgaa aaattttcac
     2701 tcggatttgg caagaagcta tttggctttc gcagagggga gaccgaatac caggtctcaa
     2761 tgctccctct tggcgggttt gtaaagatgt atggtgagac cggagaggga tgcataattg
     2821 tagaagacat aaaggaaggc tccaaggcag agaaactcgg gcttatttcc ggtgataaga
     2881 taatgaagat tgacggaaat gagctaaaag agttttctac ttggaagagt ttacttcaca
     2941 ggctcaagac atccgataag acagagcatg aatatttggt tgaaagagat gatgacagga
     3001 taaggcttaa cggaagcctt gaagatattg acggcatgaa cgctttttca gaaaaagagt
     3061 attcaagggg tttttccaac cagcctcttc taaacagatt tcttatagta atagctggtc
     3121 cggcaatgaa ctttcttgtg ccatttgttt ttttcccgat ggtattttta ctgggcatag
     3181 aacaaccggc ttatctggat aaggttcccg aagtaggata tatccagcct gagtcagaag
     3241 ctcaaaaagc aggctttgag atcggagata gaatcttaaa gataaacaat aaacctgtaa
     3301 acacatggaa agaagctact atagctttcc agatcaaccc tgactcccta cttagtgtta
     3361 cagtggatag ggccggaacc gaaaaagagc tgtcggtaaa agccatagcc ggaaaccagg
     3421 gagtagtatc aataggtgta ctggaacctt tggatgccgt agtaggaaat gtcggccccg
     3481 gaacacctgc aaaagatgca ggtctacaaa aaggtgacaa aattatacaa atcgatgatg
     3541 tcaagattaa tgactggtat caaatgtcgg ctgctattaa gaagaggggt gacaaagaat
     3601 caacatttat cattcttaga gatgacaaag aacttagtat tcaaatcact ccagcactaa
     3661 aagaaaacga tcaggggcaa attggcatag gccctcttca ggaacaaact gtacaaaaat
     3721 atggcctttt taaatctata accaagggaa tgctaaaggc atatcatgat attatagaaa
     3781 ttaccgaatt attgtttgga tttttatata aacttataac tggcgcaata ccacttagta
     3841 cggccggaaa aaccattgca ggccctctgc tgattgctca ggtttcaggg gcagcggcgg
     3901 agagcggctg ggctacactc ttgcaattta cctcttttat aagtatcaac cttgctataa
     3961 taaacctttt tcctattcca atgctagatg gcgggcatgt tgtttacata ggacttgaag
     4021 ctgtaagaag gagacctctt agcgaaaaag caatggaata cagccagaaa gttggattta
     4081 cttttttaat actactgatg tttctggcag tttttaatga tgtaagcagg ctaaaaaacg
     4141 acattataaa gccgtttaaa aaggcagccg aatatataaa atagttatat tgtatacttg
     4201 ctaaatgagt cttcttaaat taggaaaaga caactgggca agaacttctc ccacaaagct
     4261 tgggatttta tatattgcac tcactctctt tgtgggattt gccgctatta atacgggcaa
     4321 taaccttctg taccttacct ttggagtcat gttaagtttt gtgatagttt ctggcatcat
     4381 atctatgttt aaccttgcga ggatagatgt tgatttcaaa attccaaggg atgtttatgc
     4441 tctaagttct actaaccttg ttttcagtct tagaaataac aaaccgctta tcccctctta
     4501 ttcattaaca attgacctaa atggcagaaa agcctttgtt ccgtacttaa aaagagatgc
     4561 taaactaaaa actaaccttt tatatgtttt cagtaaaagg ggctggaaca caatccccga
     4621 tgccactatc tctacaagtt tcccttttgg attttttaaa aaatggataa gaattgacct
     4681 tgggaaaaag gaagtcctag tatatccaaa gattgaaaag attgatatat cagacagttt
     4741 catacaggag aagaagggag agaatgaaat agataaaaaa ggctttgggg ccgacctcag
     4801 gtcaataaaa gatttttctc agggagataa ccccaaattt attcactgga aagtatctgc
     4861 caagaaaaac aagcttatgt tcaaagagat gcaggatgaa gaatcagaat ctgtaacact
     4921 gcagtttaat ccaaccaaaa acgaagggga gcttgaaaga cagatagtac aaattgcatc
     4981 taccttgatg gaacttctaa gaaaaaatta tctggtcgat tttatttccc cacaaaaaac
     5041 ctatccacac tcacaaatag gaaacccgcc aagggaagtg ctttcatacc tggcacttta
     5101 taacaattaa tctatttatt ctttttggat ttagtagcca tttccacaag cgggcgcctg
     5161 ccgatattgt cctttttgaa acactcaatg atatgctcag cttcttttaa aggaacagta
     5221 ataaaggaaa acttttcaaa gacctgcaca tcatcaatgc ttctgtcttc cacgtttgct
     5281 ttgtttttta taaagcttac cagcttcctt ttattcatgt cgtccttttg accccttgca
     5341 ataaaaagtc ttgttttacc ctgtatcttg ggataaacat ctcttacctc attatatctc
     5401 tgggggttga ggtcatcact aaaagtggtc tgcaatagcg aagcaataag ttgttccggg
     5461 tttgtttcgc ctatcatttc acgcgcaaga tcggaaaatt cctgctgtat attagcatta
     5521 tagatatcat ttatcctgtc tttaatcctg tcttttttta cctctataat atcatcgact
     5581 ttaggtagtc tttccctgcg gatatcggtt ttggctgcct ttttaatatg cattaatttt
     5641 ctatattctt cgggggttat aaatgtgatg gctgttccag attttcctgc acgccccgta
     5701 cgtccaattc tatgcacata tgactccggg tcctggggga ttgaataatt tattacatga
     5761 gaaagattat caatatctat gccccttgct gcaacatcgg tggcaataag aatattaatt
     5821 cttttatctt taaaacttgt taatatcttt tctcttctgt gctgtgaaat atctccatgt
     5881 agaccttcgg tatcatagcc cctgtcttta aggtggtttg caaccgtatc cacatcaatc
     5941 tttgttctac aaaaaataag gccataaaat tccttttcaa agtcaattat tctgcaaagt
     6001 gcttcaaatt tatctcttcc gggtacttca aaatatatct gatcggtgag atccaccgtg
     6061 attttttctg tttcggtttt aattagttca tagcccttca tgtgcctttt tgcaacggcc
     6121 attatctctc ttggcatcgt tgcagaaaaa agcagggttg tcctttcttt attggttgct
     6181 gcgagaattt cctcaacatc gtcaataaat cccatgttaa gcatctcgtc agcttcgtct
     6241 agtaccgcat atgaaacatt ttcaagcttt agagtaccgc gttttatatg gtcgagcacc
     6301 ctaccaggag tacccactac cacttccacg ccttttttca gtcttttgag ctgaagctcc
     6361 atagactgtc cgccgtaaat agggatcacc tgaatcttct ttctgccctt aagagaattt
     6421 atttcttcag aaacctgaat ggcaagttct cttgttggag taagaatgat cacctggaca
     6481 tactttgtat tctcttcaat attttcaagt atcggaatac caaatgccgc ggttttgccg
     6541 gtgccggtct gcgcctggcc cacaaggtcc ttgttggcgc tcaatagaat tggaattatt
     6601 tttacctgaa taggtgtagg ctcttcaaag cccttctttc tcagtgccgc aagcatctcc
     6661 tgcgatacac caagattttc aaatttttct agttcactca tatatatcct gcaataaaaa
     6721 atgtcggggg ttgaattaaa ggattaacca aaaagaattg ttcgatagtt tttcgctcta
     6781 taatattcaa atcagtatta cttcttttcc cagcagttat aaggatactt gactaaatta
     6841 gaattactat acctgatatc atttgtcact tcatctttta tccatgaagg taaaacaact
     6901 ttttcattct cgtcactcat ttcaacctcg gccacaataa gtcctttatt acctccttcg
     6961 aatacatcaa tttcccaagt gtggtcaaaa tgttcaatct tgtatctggt cttatcaatg
     7021 accatattat tgcaaagagt atcaataatt tcctccgctt cattatacgg aatttcatat
     7081 tcatactcag ttcgggtgat accgctccga gaacccttta ttgttatata accttttccg
     7141 cccttaatac gcaccctaat agcagtgtta ttcgttgtag gaaaataacc ctgcttaata
     7201 acctctccct ctttaaggtc agctacaata gattcatcaa ttaaaaactt tctttctatt
     7261 tctatgggca tttttcgtta atataataac ttaatatgat agatacaaac aaaatcttaa
     7321 tcattggctg cggtgtttca ggtcttacat gcggagtgaa actgcttgac agtggctttg
     7381 atgttgaaat cattgcatca aagttacccg cagatactac ttcgagcgtg gcgccggcat
     7441 attggtaccc gtataaagtc tatcctgaag agaaggtgct aaaatgggga gagatttcat
     7501 acagagaata tgcaacactt agcaacgata aaaataccgg ggttactttt aaaaagcttt
     7561 tcaaattatt tgagaagaaa gtacccagac cttactggac aaagattgta aaaagtttta
     7621 atgaagtaca gaaatccgaa ctgcctggtg gatataatta tggtttcact gtcgaagtgc
     7681 ctcaggttga agcgcccatt tatttaaaat atatgtacaa caggtttata gagttgggag
     7741 gtttggtaac tcaaattgaa gagttaacga gcctagagca tttatataat gacaacagga
     7801 ttattgttaa ctgcacgggt ctcggttcat ataaactgtg cagtgacagt gagatgtttc
     7861 ctatacgcgg ccagcttgta agaacatcaa atccgggcgt ggacaaaata tataatgatg
     7921 aagatgggcc gcttgctatc acttatatag tcccaagaag tgatgattgt gtactcggtg
     7981 gtactgcaca ggaaaacaac tggaatctgg aagtggataa atctatatct gatgccatat
     8041 tatcaaaatc agctacactc gtaccagagt taagagatgc agatattctg gaacataaag
     8101 tcggactcag gcccggaagg acagaagt
//
LOCUS       DBNGSV010000303         9683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1716953, whole genome shotgun sequence.
ACCESSION   DBNGSV010000303 DBNGSV010000000
VERSION     DBNGSV010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1716953"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC159U_11020"
     CDS             complement(<1..682)
                     /locus_tag="AC159U_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012563.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR3337622.1"
                     /translation="MNNTDYDEFRCSFMKYLKISRGKDIKDINKEDICHALTLTLKEL
                     IIDNYLNTLQRYKDNKVKRLYYISIEYMIGSLLKNNISNLNLDDVCESLVSWLGYSLD
                     EILEQDNEPALGTGGLGRLAACFLDSLASMNMPGFGYGINYEFGSLKQIVKDGYQVEM
                     PDDWCRDKSPILIDKLAETIKVPVFGHLEKTKDQDGCNRGVWKNYIYINGIPSDLPIV
                     GYGGKTVNS"
     gene            855..2774
                     /gene="glgB"
                     /locus_tag="AC159U_11025"
     CDS             855..2774
                     /gene="glgB"
                     /locus_tag="AC159U_11025"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR3337623.1"
                     /translation="MTTYEMIHGVSLFSDFDIDLFKSGHHFKLYEKLGSHPMEVQSTQ
                     GVYFSVWAPNAKKVSVIGDFNWWNSESHPLANRWDGSGIWEGFITGIEKGCLYKYKVV
                     SNYYNYSAEKSDPFALLSETPPKTASVVWDLDYKWKEKKWEKSRHDRNSLNSPISIYE
                     MHIGSWKKVTEHGDRSLGYRELSEQICEYVKKLGFTHVEFMPVMEHPFYGSWGYQTTG
                     YFSATSRYGVPQDLMFLIDSLHDEGISVILDWVPSHFPTDEHGLGFFDGTHLYEHSDP
                     MKGFHPDWKSYIFNYDRNEVRSFLISSAHFWFDRYHIDGLRVDGVASMLYLDYSREQG
                     KWIANEYGGRENLGAISLLKELNESIYNDFPHAQVYAEESTAWPGVSRPTYSGGLGFG
                     FKWNMGWMHDTLKYFEKDPIYRKYHQNDITFSILYAFTENFVLPLSHDEVVHGKGSLL
                     SKMPGDDWQKFANLRALYAYMWAHPGKKLLFMGGEFGQWEEWNHNTSLDWHLMEFEPH
                     NGITNLISDLNRFYKNESALYEYDLSHEGFEWVDNSDHNSSVLCFLRRNSKNKIILVV
                     CNFTPTPRHQYRIGVPYAGDWKESLNTDSTIYGGSGIGNAGLAVATEMPWHFRPYSIC
                     ITLPPLSVLYLLHDD"
     gene            2777..4933
                     /gene="glgX"
                     /locus_tag="AC159U_11030"
     CDS             2777..4933
                     /gene="glgX"
                     /locus_tag="AC159U_11030"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3337624.1"
                     /translation="MPRIIDNNGKNIEIWPGKPYPLGAIWDGSGVNFALFSENADKVE
                     LCLFDNSETETARIPIIEHTDQVWHIYLPQARPAQLYGYRVYGPYDTENGLRFNPSKL
                     LLDPYAKSISGTINWDDSLFGYKINGTENDCVVDNTDSAKFIPKCSVIDTSFTWGNDS
                     SPQIPWHKTIIYEIHVKGLTAEHPDVPEQLRGTYSALTYEPLIEYFHSLGITALELMP
                     IHHFVSDRHLVKAGLSNYWGYNTIGYFSPDPRYCTRGIFRSQVEEFKTMVKTLHREGI
                     EIILDVVYNHTGEGSHLGPTLCFRGIDNKCYYRLNPDDPRYYLDYTGCGNTLNTNHPR
                     VLQLIMDSLRYWVLEMHVDGFRFDLAATLSRGKNGRDRLASFFDIIHQDPVLSQVKLI
                     AEPWDLGHDGYQIGNFPVLWAEWNGKYRDTVRKYWKGTDNTISDLAYRLTGSSDLYEQ
                     TGRRPYASINFVTCHDGFTLNDLVSYNEKHNESNLEDNRDGTNNNDSNNYGAEGPVKN
                     EDIIETRERQKRNFLATIFLSQGVPMLLAGDEIGRTQLGNNNAYCQDNQISWVNWSLD
                     KKKSDLLNFVKYMIEIRKSNPVLTRKKFVRGQITNGSHYKDITWYRTDGTEMTHEDWT
                     NPITMCMGLKLNGEAIEEYDEKGKIITGNTLLILLNSYWSDLQYVLPSKKSKGAWELI
                     MDTRYPDGKTEQKHFKYGQKYEILGRSVSLFKLTNK"
     gene            4945..6450
                     /gene="malQ"
                     /locus_tag="AC159U_11035"
     CDS             4945..6450
                     /gene="malQ"
                     /locus_tag="AC159U_11035"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR3337625.1"
                     /translation="MNNKKCGILLHITSLPSQFGIGDLGKYAYDFADFLLKAKQTYWQ
                     VLPLNPTLDIHGNSPYSSFSAFAGNPILISPELLYEDGFVSRSDLMPNYEQNAEKVKF
                     NHVYEYKNQILEKAYLSFSSLSKKPEEYNKFISENKSWILDYVLFVSIKDNSKGVIWN
                     KFPEGLKYRDKQALDSLSEKLKEKIEKEIFVQYVFFKQWYKLKDYCNKNGIKIIGDLP
                     IYLNYDSSDVWSNPKNFKLRNDRTPEFLAGVPPDYFSKTGQLWGNPVYEWEYIKDSGF
                     KYWDDRISHNLKLFDILRLDHFRGFVNYWEVPAGEETAVNGNWVNAPAEQFFTHLLRQ
                     FDNKRFIAEDLGLITQDVLEIRDKFEFPGMKVLQFAFRDDYPNGAFLLHNHPYNCVVY
                     TGTHDNNTTLGWWTNECTDVQKQRVKEYMQNNKIDTDVAINWKFIKLALSSKAHTVII
                     PMQDYLSLGQEARMNTPSVSNNNWEWKISDNYNYTCIAEIIRENTEFFERS"
     gene            6486..7970
                     /locus_tag="AC159U_11040"
     CDS             6486..7970
                     /locus_tag="AC159U_11040"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR3337626.1"
                     /translation="MLNLLFVAAEMDPLVKVGGLGDVMWGLPKKLKDNDCKIRIVLPN
                     YRTVKENIESMNLESVDKEMKFKIDISGLKFEFKITQYRLNDIRLILLDNEYFFDREH
                     VYCTPHGDYEDNYLRFGAFCLASLEASRILKFKPDIIHCHDWHTAFIPIFLKNSTNLI
                     RHHSYFENSKIVFSIHNIAYQGVYDMHQFKIFKFASYLYSPFGIEYYGKINIMKGGII
                     YSDLVTTVSPTYSKEIKTPLIGKGLEGVITDISERRNNLVGILNGIDYDKWNPETDKN
                     LYYNFSLDDLSGKLRNKFDLKAQFNLSTGNDKPLIGMVCRLAEQKGVDLVIESLERII
                     KMGFQLIIIGSGEQRYIDMIDEQNKIHEGNFSGLVKYNDKIARRIYAGSDIFLMPSRF
                     EPCGLGQIIALRYGTIPVVRKTGGLIDTIIDYPSHINNGYGFIFDEFSIADLLSCLRK
                     VYKVYGDSEQWLTLIRRAMKVRFSWDDTSKIYIHEYKKLMELKF"
     gene            complement(8211..8912)
                     /locus_tag="AC159U_11045"
     CDS             complement(8211..8912)
                     /locus_tag="AC159U_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3337627.1"
                     /translation="MKKISTMHSVLVSDFVYGGIDGAVTTFAVVAGVSGASLSPGIVL
                     VLGFANLFADGFSMAVGNYLGTKSHIEFANKIRRAEENSIIEKPEEEREEIHEIYKQK
                     GFSGDHLQKTVDVITSNKKIWIDTMMTDEFGIIEETKSPIKAALTTFIAFNIMGIIPL
                     FSFILYYFFPFFKESAFITSIIFTSIALFLVGSIKANIVDKKWFLSGLETLIIGGVAA
                     SIAYFIGFWLKGLAG"
     gene            complement(9029..>9683)
                     /locus_tag="AC159U_11050"
     CDS             complement(9029..>9683)
                     /locus_tag="AC159U_11050"
                     /inference="COORDINATES: protein motif:HMM:NF023045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3337628.1"
                     /translation="ANKAGDCIDCYKCVSVCPTGIDIRSGLQMECVGCTACIDACDEV
                     MEHIDKPKGLIRYVSANSLNGFTSKLLKPRIAVYSVLLILIFSLLIINVSKRQDLLVT
                     MLRGKDVPYQVVQEGSDKKIINHFKLHLKNQTFEDSIIQLKISEKWNDGQIEIISQNK
                     DIQIFAGKDITVHFFLKFPYEVINETGTKSIEIDFIDLQTKEIKSKKDIKLIGPLSG"
BASE COUNT         3216 a         1762 c         1867 g         2838 t
ORIGIN      
        1 ggctgttaac tgttttccct ccataaccca ctataggcag gtctgagggg ataccattta
       61 tgtatatata attcttccat actcccctgt tgcatccgtc ctgatccttg gttttttcga
      121 gatgcccaaa tacaggaacc tttatagttt ccgccaattt gtcaatcaag atgggagatt
      181 tatccctgca ccaatcatcc ggcatttcaa cctgatagcc gtcctttacg atctgtttga
      241 gtgaaccaaa ctcatagtta ataccgtaac caaatccggg catgttcatc gatgccagag
      301 aatccaagaa acatgcagcc agccttccca gccccccggt gccaagtgcc ggctcgttgt
      361 cctgttccaa tatttcatca agactgtaac caagccatga gactaaactt tcgcatacat
      421 catcaaggtt taagtttgaa atattgtttt tgagcaagct tccgatcata tactctattg
      481 agatataata gaggcgtttt actttgttat ccttataacg ctgcagagta tttaaatagt
      541 tgtctattat tagttctttt agtgtgagtg ttaaggcatg acagatgtct tctttgttaa
      601 tatcttttat gtcttttcca cgtgagatct ttaaatattt cataaagctg caacgaaatt
      661 catcgtaatc agtattgttc aaatgcatct ccttttctca acctagctat gttgattata
      721 tgaaacagat aattttaatg ttattaaaga atttgattga tattaaatat cattaatata
      781 aatttcaaat cttaattaat tgtatttata taaaatattt atataaattt aattaagtag
      841 tattacaaat acatatgaca acctatgaaa tgattcatgg cgtaagcctg ttttccgatt
      901 ttgatataga cttatttaag tccggccatc actttaagct ttatgaaaaa cttggctcac
      961 accccatgga agttcagagc actcaaggtg tgtatttctc agtttgggct cccaatgcaa
     1021 aaaaagtatc agttatagga gatttcaact ggtggaattc tgaaagtcat ccacttgcca
     1081 acagatggga tggttcgggt atatgggagg gttttataac aggaattgaa aaaggatgct
     1141 tatataaata taaagttgta tcaaattact ataattactc tgcagaaaaa agcgatccat
     1201 tcgctttatt atccgagact ccccctaaaa ccgcatctgt agtatgggat ttggactaca
     1261 aatggaaaga aaaaaaatgg gaaaaatcca ggcatgatag aaatagttta aacagcccta
     1321 ttagtattta tgaaatgcat atagggtcat ggaaaaaagt aacagaacac ggagataggt
     1381 cacttggtta cagagaactg tcagaacaga tatgcgaata tgtaaaaaaa ctaggattta
     1441 cgcatgttga atttatgcca gttatggaac acccgtttta cggctcatgg ggatatcaga
     1501 ccaccggcta cttttcggct actagcagat acggagtccc tcaggattta atgtttttaa
     1561 tagactctct tcatgatgaa ggtattagtg taatattaga ctgggtacca tctcattttc
     1621 ctactgatga gcatggtttg ggttttttcg acggtacaca cctatatgaa cattcggatc
     1681 caatgaaggg gtttcatcct gactggaaaa gctacatatt taactatgat agaaacgagg
     1741 tgaggtcatt tcttataagt agcgcacatt tctggtttga tagatatcat atagacggtc
     1801 tgcgtgttga tggagtggca tcgatgcttt atttagatta cagtagggaa caaggaaagt
     1861 ggatcgccaa cgagtacgga ggaagggaaa acctgggtgc gataagcctg cttaaagaac
     1921 tgaatgaatc gatttacaat gattttcctc atgcgcaggt ctatgcagag gaatccaccg
     1981 catggccagg cgtttcgaga cccacatatt ccggaggact tggatttggt tttaaatgga
     2041 acatgggatg gatgcatgat acacttaaat actttgaaaa agatccgatt tacagaaaat
     2101 accatcagaa tgatatcaca ttcagtatat tatacgcctt tacagagaac tttgttctgc
     2161 ccctttctca tgatgaggtt gttcacggca aaggatcatt gctgagtaaa atgccgggag
     2221 acgactggca gaaatttgct aacttaagag cactctatgc ttatatgtgg gcccatcccg
     2281 gcaaaaaact tctatttatg ggaggggaat tcgggcagtg ggaggagtgg aaccacaaca
     2341 caagcctcga ctggcatctc atggaatttg agcctcacaa tggaataact aatttaataa
     2401 gcgacctaaa cagattctat aaaaacgaat ctgcgctgta tgagtacgat ctttcacacg
     2461 aaggttttga gtgggtagac aactctgatc ataacagtag cgttttgtgt tttttaagaa
     2521 gaaattctaa aaataaaata atacttgtag tatgtaattt tactcccact ccaagacatc
     2581 agtacagaat aggcgtaccg tatgcagggg actggaaaga atcactcaat actgattcca
     2641 caatttatgg aggaagcgga attggaaatg caggattggc agttgcaact gaaatgccct
     2701 ggcatttcag gccatattct atatgtatca ccttaccccc tcttagcgtt ctataccttt
     2761 tgcatgatga ttagcaatgc ccagaataat tgataataac ggaaaaaaca tagaaatatg
     2821 gccaggcaag ccatacccgc tcggagccat atgggatggg tcgggagtga actttgcgct
     2881 tttctcagaa aatgcggata aagtggagct ttgtcttttt gataactcag aaacagaaac
     2941 ggcaagaatt cccataatag agcacaccga tcaggtatgg catatatacc ttcctcaggc
     3001 aaggccggca caattgtatg gatacagggt ttatggccca tatgataccg aaaacggtct
     3061 gaggtttaac ccatccaagc tgcttttaga tccgtatgca aagtctattt caggtaccat
     3121 caactgggac gacagcctgt tcggatataa gattaatggc acagaaaatg actgtgttgt
     3181 tgataatacg gatagtgcta aatttattcc caaatgcagt gttatagata cgtcttttac
     3241 atggggcaac gactctagtc ctcaaatccc atggcataaa accataatct atgaaatcca
     3301 tgtaaaaggt ttaactgccg agcaccctga tgttccggaa cagcttcgag gtacatattc
     3361 agcacttact tatgaacctc taatagaata ttttcattct ctgggcatta ctgcactcga
     3421 gcttatgcca attcatcact ttgtttccga cagacatctg gtaaaagcag gactttcaaa
     3481 ttactgggga tataacacaa ttggatattt ttctcccgac ccgcgttatt gcacccgggg
     3541 tatattccga agccaggtag aagaatttaa aacaatggta aagactctgc accgcgaagg
     3601 tatagaaata attctggatg ttgtatataa ccataccgga gaaggaagcc accttgggcc
     3661 tacactgtgc ttcagaggca tagataataa atgttattac agacttaacc ccgatgaccc
     3721 gagatattat ctggactata cgggatgcgg aaacacactc aatacaaatc acccccgggt
     3781 actgcagctt ataatggata gcctcagata ctgggttttg gagatgcatg tagatggttt
     3841 tcggtttgat ctggcagcaa cactttcaag aggaaagaac gggcgtgaca gactagcatc
     3901 attttttgac ataattcatc aagaccctgt tctgtctcag gttaaattaa ttgccgaacc
     3961 atgggacctc ggacacgacg gttatcagat aggaaacttt cctgtactgt gggcagaatg
     4021 gaacggaaag tacagggaca cagtgagaaa atattggaaa ggaacggata acacgatatc
     4081 tgatcttgca tacaggctta cggggtcaag cgatttatat gagcagacag gaagaagacc
     4141 atatgcaagc ataaactttg taacatgcca tgatggcttt accctaaacg atttagtaag
     4201 ctataatgaa aaacacaatg agtccaattt agaagataac cgtgacggca cgaacaataa
     4261 cgacagcaat aattacggag cggagggccc ggtaaaaaac gaagatatta ttgaaaccag
     4321 ggaaagacag aagagaaact ttctagctac tattttcctc tcacagggtg taccaatgct
     4381 tcttgcggga gatgaaatcg gaaggaccca gcttggcaat aacaatgcct actgccagga
     4441 caatcaaata tcctgggtaa actggagcct ggacaaaaag aaaagtgatt tgctgaattt
     4501 cgtaaaatat atgattgaaa tcagaaaaag caaccccgtg cttacaagaa aaaaatttgt
     4561 tagagggcaa ataacaaatg gtagtcacta caaagacata acatggtaca gaacagatgg
     4621 tacggagatg acacatgagg actggacaaa tccgattaca atgtgcatgg ggctcaaact
     4681 caatggggag gcaatagaag agtatgacga aaaagggaaa attataacag gcaatacgct
     4741 gctaatatta ttaaactcct actggagtga tcttcagtat gttctaccat ccaaaaaatc
     4801 aaaaggcgcc tgggaactaa ttatggacac ccgctacccg gacggaaaga ctgaacagaa
     4861 gcattttaaa tacggacaga aatatgaaat actgggcaga tcagtaagtt tgtttaaact
     4921 tacaaacaag taagtcaaat ctaaatgaac aataaaaaat gcggaatact cctccatatt
     4981 acatcccttc catctcaatt tggaatagga gatctaggca aatacgcata tgattttgca
     5041 gattttttat taaaagcaaa gcaaacctac tggcaggtac tgcctttaaa tccaacactt
     5101 gatatacatg gcaattctcc ttacagcagt ttttccgcat ttgcaggaaa tcctattctt
     5161 ataagtcctg aactgctgta tgaagacggg tttgtatcaa ggtcagattt gatgcccaac
     5221 tatgaacaga acgcagagaa ggttaaattt aatcatgtat atgaatacaa aaatcagata
     5281 ttagaaaaag cgtatttaag cttcagttct ctttccaaaa aacctgaaga atacaacaaa
     5341 ttcatttcag agaataaaag ctggattttg gattatgtgc ttttcgtatc aataaaagat
     5401 aattccaagg gagtaatctg gaataaattc cccgaggggc ttaaatacag ggacaaacaa
     5461 gctctcgatt cattaagtga aaaactaaaa gaaaaaatag aaaaagaaat tttcgtgcag
     5521 tacgtatttt ttaaacagtg gtataaacta aaagactatt gcaataaaaa cggcataaag
     5581 attataggtg accttcctat atatctgaat tatgacagct ccgatgtgtg gagcaatcct
     5641 aaaaacttta agctaaggaa tgatagaacc cctgaatttt tagcaggggt tccgcctgat
     5701 tatttcagca aaactgggca gctctgggga aatcctgtat atgaatggga atatataaaa
     5761 gactctgggt ttaaatattg ggatgaccgc atatcacata atttaaaact atttgatatc
     5821 ttaaggcttg atcacttcag gggatttgtc aattattggg aagtacccgc tggtgaagaa
     5881 accgcggtta acggcaattg ggttaatgct ccggcagagc agtttttcac tcatttacta
     5941 aggcagtttg ataacaaaag gtttattgca gaagatttgg gattaatcac acaggacgtt
     6001 ttggagatta gagataaatt tgaatttccg ggaatgaagg tattgcaatt tgcgtttcgg
     6061 gatgactacc caaacggagc ttttttgctt cacaaccatc cctacaactg tgttgtttat
     6121 acaggaactc atgacaataa cacgacgcta ggatggtgga ctaacgagtg cacggatgtt
     6181 caaaagcaaa gagtcaagga atatatgcaa aataataaaa ttgacaccga tgtggctata
     6241 aactggaaat ttataaagct ggccttgagt tcaaaagctc ataccgtaat aatacctatg
     6301 caggattatt tatcacttgg acaggaagca cgaatgaata ctccttcagt ttccaataac
     6361 aactgggagt ggaaaatctc tgataattat aattacactt gcatagctga aattattaga
     6421 gaaaatacag agttttttga acgaagttaa atattagtcg aatttacgac ctggggtgaa
     6481 ataaattgct gaacctatta tttgttgctg cagaaatgga cccgcttgtt aaagtaggcg
     6541 gccttggtga tgttatgtgg ggtctgccta aaaagctaaa agacaatgac tgtaaaattc
     6601 gtatagtgct accaaattac agaacagtta aagaaaatat tgagagtatg aatcttgaat
     6661 ctgtagacaa agaaatgaaa ttcaagatcg atatatcagg tctaaaattc gaatttaaaa
     6721 taactcagta taggcttaac gatatcagac tcattctgtt ggataatgaa tatttttttg
     6781 atagagagca tgtatattgt actcctcatg gagactatga agataattac ctaagattcg
     6841 gcgctttctg tttggcatct ctcgaagcct caagaattct taagttcaag cccgatatta
     6901 ttcactgcca tgactggcat actgctttta tcccaatatt tctaaagaac agcactaacc
     6961 tcataagaca tcattcatac tttgaaaatt ctaagattgt attttctatt cacaacattg
     7021 catatcaggg agtgtacgat atgcatcaat ttaaaatatt taagtttgcg tcatatcttt
     7081 attcaccttt tggcatcgaa tactatggca agattaatat catgaaagga ggaattattt
     7141 attcagacct ggtaaccact gtaagcccta catacagcaa ggaaattaag actcctctta
     7201 tcggaaaggg actcgaagga gtgatcaccg atatttcaga aagacgtaat aatctcgtag
     7261 gaatcctaaa cggaattgac tacgacaaat ggaaccccga gacagataaa aatttgtatt
     7321 ataatttcag cttggacgat ctcagcggta agctcagaaa caaattcgac cttaaagcgc
     7381 agttcaatct tagcacaggt aatgataaac cgcttattgg tatggtatgc agacttgcag
     7441 aacaaaaagg tgttgatttg gttatagaat cattagaacg aataattaaa atgggtttcc
     7501 agttaattat tataggctcc ggagaacagc gatatataga tatgattgat gaacagaata
     7561 aaatacatga aggcaatttc tccggtcttg tgaaatataa tgacaaaata gctagaagga
     7621 tttacgcagg ttcagacata tttcttatgc cttctaggtt tgaaccctgc ggtcttggac
     7681 agatcattgc tcttagatac ggcaccatcc ctgtggtaag aaaaacagga ggacttatag
     7741 atacaattat tgactatcct tcacatatta ataacggata cggttttata ttcgacgaat
     7801 tcagcatagc agatctttta tcctgtctta gaaaagttta taaagtctat ggtgattctg
     7861 aacagtggct taccttaata agacgtgcca tgaaggtgcg tttttcatgg gatgatacaa
     7921 gcaagatcta tattcatgag tacaaaaaac tcatggaact aaaattttga agcaagtgaa
     7981 gtatatacaa aataatttaa catatattaa ataagaaaat tattatatgt aattctcagt
     8041 atattcgtaa gtaactgaat tggctattgc aggaaaacag ggaaagatac aggaaaatac
     8101 taaaataacc ttcaggacgc gaagaccgcg ggttcgagct cagtgtcaca ccgagactgc
     8161 atactataat taatattaaa cagcttttga tccatgaaat ttctaattaa ttatccagcc
     8221 aagcccttta accaaaaacc aataaagtaa gcaatcgatg ccgctactcc tccaattatc
     8281 agtgtttcca agcctgataa aaaccatttt ttatctacta tgtttgcttt aatggaaccc
     8341 acgagaaaaa gagcaataga ggtgaagatt atagaagtga taaatgcaga ttctttaaag
     8401 aatgggaaaa agtaatatag tataaacgag aaaagcggta ttattcccat gatattaaag
     8461 gctataaaag tcgtaagcgc tgctttgatg ggtgatttag tctcttctat aataccaaac
     8521 tcatctgtca tcatcgtgtc aatccagatt tttttattac tggttatcac atcaactgtt
     8581 ttttgcaaat gatcacctga aaaccctttt tgtttataaa tctcatgtat ttcctcgcgt
     8641 tcttcttccg gtttttcaat gattgagttt tcttcagctc ttctgatttt atttgcaaat
     8701 tctatatgag atttggttcc aagataattc cctacagcca tggaaaatcc gtcggcgaat
     8761 aaatttgcaa atcctaaaac aagcacaatc ccgggcgata gagaagcacc ggatacccct
     8821 gctacaaccg caaaagttgt aacagcgcca tcaatacctc cgtatacgaa atcggacact
     8881 aaaacactgt gcatagtaga tatcttcttc attttatgag ccaaaaaatc taaattaatg
     8941 attaaatatc atttattatt atattagaat ttagcgtaat tcctttatat tattacatta
     9001 tgttgctgaa ttatctacta agcttacact atccggacag cggcccaatg agttttatat
     9061 cttttttgga cttaatttct ttagtttgta aatcaataaa atcgatttct atcgatttcg
     9121 tgcccgtttc atttataact tcataaggaa attttaaaaa gaaatgaacc gtaatatcct
     9181 tacccgcaaa aatttggata tccttatttt gtgaaatgat ttctatctga ccatcattcc
     9241 atttctctga tatttttaat tggattattg aatcctcgaa tgtctgattt ttaagatgta
     9301 gtttgaaatg attgataatt tttttatcac taccttcctg cactacctga taaggtacat
     9361 cttttcctct gagcatggta accaacaaat cctgtctttt ggaaacatta attattaata
     9421 gtgaaaaaat tagaattaaa agtaccgaat atacagctat tcttggtttt aataattttg
     9481 aagtaaatcc atttaagctg tttgccgaaa catatcgtat caggcctttt ggtttatcaa
     9541 tatgttccat aacttcatca catgcatcaa tgcatgccgt gcatcccacg cattccatct
     9601 gcagaccact cctaatatct atccccgtag gacagaccga tacgcattta tagcagtcaa
     9661 tacagtctcc tgctttatta gct
//
LOCUS       DBNGSV010000304         2760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_816711, whole genome shotgun sequence.
ACCESSION   DBNGSV010000304 DBNGSV010000000
VERSION     DBNGSV010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_816711"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1076)
                     /gene="era"
                     /locus_tag="AC159U_11055"
     CDS             complement(171..1076)
                     /gene="era"
                     /locus_tag="AC159U_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010173715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3337629.1"
                     /translation="MNESNDSDNFRSGFVTIVGRPNVGKSTFINKVVGMKIAAVSDKP
                     NTTRNRILGIKNLDDAQIVFLDTPGMTDGKGVIGRSMVNVVASAVYESDIVLMIVDVK
                     DAFTEADKKIIENQTKPIILLINKIDAFKKSRILNVIAQAQEYEGKFLDIIPVSALKG
                     DGLDIVFNTIKSNLPQGPRYFSEDQVTDQTERFIVSEIIREKIFELTHKEIPYRSAVV
                     VDEFDEDEKKKIINIHATVYVERPNQKGIIIGDKGSLMKEIGTRARKDIEELLGIKVF
                     LQIWVKVKDNWTSKEHLIKDFGYCG"
     gene            1178..>2760
                     /gene="aspS"
                     /locus_tag="AC159U_11060"
     CDS             1178..>2760
                     /gene="aspS"
                     /locus_tag="AC159U_11060"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3337630.1"
                     /translation="MLEELGNWKRSYYCGELRKENDGEEVILMGWVQSYRDHGGVIFI
                     DLRDRTGLSQVVFNPDVSKESHEKAEHIRDEWVIAIKGKVRPRTPETINPNLPTGEIE
                     IFVDETKIINRSEVLPFQIEDEVQVDELIRMKYRYLDLRRNEMKNNLMFRHKVVSTTR
                     NYLDAGGFVEVETPFLTKSTPEGARDYLVPSRQSEGEFYALPQSPQLLKQTLMVSGFD
                     KYYQIVKCFRDEDLRADRQPEFTQIDLEMSFVDENDVIGLIEGLIVDIMSKTLGKDIE
                     LPFARITYEQSVLRYGTDRPDTRFGLELVDITDVFKDSEFKVFKTAIKKGGILKALNL
                     KGKADELSRKDIDGLTEYANSLGAKGLAWIKVSGDEWQSPIIKFFSDEEKAGLKESLN
                     MEDGDIVFFGADSPYIVNLVLSNIRLELGKKFDLIDEDKFNFLWVTDFPLFDYDEKEK
                     RYVALHHPFTSPNTQDLDLLETEPLKVKSRAYDIVLNGIEIGGGSIRIHRKDIQQKIF
                     RAIGMDEQEANSRFKFLLEA"
BASE COUNT          879 a          547 c          542 g          792 t
ORIGIN      
        1 tcatatataa ccaagaacta agtatctata taacaatcta ttagactgta ataataaaat
       61 tctacagact tatccaatag agctactgtt ttgtagacta taataagaat tttctaatat
      121 tagaaaaaac aagaaaaatg aagcaaccag tgcaaactta tgattagaac ttaaccacaa
      181 tacccaaaat ctttaataag gtgttctttt gaagtccagt tgtcttttac tttaacccag
      241 atttgaagga agacttttat gcccagtagc tcttcaatgt cttttcttgc acgagtaccg
      301 atttctttca tcaggctgcc cttgtcgcct attataatcc ctttttggtt gggtcgttct
      361 acgtagactg tggcatggat gttaataatc ttctttttct catcttcatc aaattcatca
      421 accaccacag ccgagcggta cgggatttcc ttgtgcgtaa gctcaaatat tttctccctt
      481 atgatttctg aaactataaa cctctcggtc tgatccgtaa cctgatcttc tgaaaaatat
      541 ctaggtccct ggggaagatt tgattttatt gtattaaaca caatatcaag cccgtcacct
      601 tttagcgcag atacggggat gatatccaaa aacttaccct catactcctg tgcctgggct
      661 attacgttta atatcctgct ctttttaaac gcatctatct tgtttattaa aagtatgatc
      721 ggctttgtct ggttttctat gatctttttg tcggcttcag taaaggcgtc cttcacgtcc
      781 actatcataa gcacaatgtc agactcatag accgctgatg ccactacatt taccatcgat
      841 cttccgatca ctcccttgcc gtctgtcatc cccggggtat cgagaaatac tatctgggca
      901 tcgtctaaat tcttaattcc caaaatcctg tttcttgtag tattgggctt atccgaaacg
      961 gctgcaatct tcattcccac aactttatta ataaacgtag acttacccac atttggcctt
     1021 ccgactatcg ttacaaagcc cgatctgaaa ttatcactat cattgctctc attcattata
     1081 tatattaatc ctcttaatta aattcatgct tgctatcaat gttaattagg ttaacatttt
     1141 cagccttaat tagtatctta aaggagcaaa taattttatg ttagaagaat taggaaactg
     1201 gaagagatcg tattattgtg gagagctaag gaaagaaaat gatggagaag aggtaatcct
     1261 catgggatgg gtgcagtcct acagagatca tgggggtgtt atttttatag acctcagaga
     1321 cagaacggga ctctcacagg tggtattcaa tcctgatgta agtaaagaat ctcatgaaaa
     1381 agccgagcac ataagagacg aatgggtaat tgccataaaa ggcaaggttc gcccgagaac
     1441 cccagaaacc ataaatccca acttacccac cggtgaaatc gaaatatttg ttgatgagac
     1501 gaagataata aacaggtcag aagtccttcc cttccagatt gaagacgagg tacaggttga
     1561 tgaacttata cgaatgaagt acaggtatct tgacctcaga agaaacgaga tgaaaaacaa
     1621 cctcatgttc agacacaagg ttgtgagcac aacaagaaat tatctggatg ctggtgggtt
     1681 tgtagaagta gaaaccccgt ttcttacaaa gtccaccccg gaaggggcaa gggactatct
     1741 ggtgccaagc agacagagtg aaggagagtt ctatgcccta ccccagtctc cgcagcttct
     1801 taaacaaacc ctcatggtat ctggttttga taaatactat cagatcgtaa agtgctttag
     1861 agacgaagac ttaagggcag accgtcagcc tgagtttact cagatcgacc ttgaaatgtc
     1921 gtttgtagat gaaaacgatg tgataggtct cattgagggg ctcatagtcg atataatgtc
     1981 taaaactctt ggaaaggata ttgaactccc ttttgccaga ataacatatg agcaatcagt
     2041 tctcagatat ggcacggaca ggcctgacac cagatttggg cttgagcttg tggacataac
     2101 agatgttttt aaggactcgg agtttaaagt atttaaaaca gcgataaaaa agggaggcat
     2161 tctaaaggcg cttaatctaa aaggcaaagc cgatgagctt tccagaaaag atatagacgg
     2221 ccttactgaa tatgcaaatt ctcttggagc caagggactt gcatggatta aagtatccgg
     2281 cgatgagtgg cagtcaccca taattaaatt ctttagtgat gaagaaaaag caggactaaa
     2341 agaaagcctt aatatggaag acggtgatat agtatttttt ggcgccgact ctccatatat
     2401 agtaaatctg gtgctatcaa acatacgcct tgagcttgga aagaaatttg atttgataga
     2461 cgaagacaaa tttaactttc tttgggttac cgactttcct cttttcgatt atgatgaaaa
     2521 agaaaaaaga tatgtagccc tgcaccatcc tttcacttca ccaaatactc aagacctgga
     2581 cctattggag actgaaccgc tcaaagtaaa atcacgagca tacgatatag tgctaaacgg
     2641 aatagaaata ggcggaggaa gcataaggat tcacagaaaa gatatacaac aaaagatttt
     2701 cagagcaatc ggcatggatg agcaagaagc aaacagcagg tttaagtttc tgctcgaagc
//
LOCUS       DBNGSV010000305         7524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1647277, whole genome shotgun sequence.
ACCESSION   DBNGSV010000305 DBNGSV010000000
VERSION     DBNGSV010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1647277"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..300)
                     /locus_tag="AC159U_11065"
     CDS             complement(124..300)
                     /locus_tag="AC159U_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337631.1"
                     /translation="MFKWILARGSEASTWRGLVALLTAVGLSISPEQTTAIVAGGLGL
                     IGAIGAFFSDKKAD"
     gene            complement(315..473)
                     /locus_tag="AC159U_11070"
     CDS             complement(315..473)
                     /locus_tag="AC159U_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337632.1"
                     /translation="MKYALVLLAGLFLVGCEASGEKKPACPEGEAENRWGLCIPVPIV
                     DFNKDRKI"
     gene            complement(484..1704)
                     /locus_tag="AC159U_11075"
     CDS             complement(484..1704)
                     /locus_tag="AC159U_11075"
                     /inference="COORDINATES: protein motif:HMM:NF044553.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich domain-containing protein"
                     /protein_id="HIR3337633.1"
                     /translation="MAKLTLTDIVAGYGSTAAYNANNALIEAALDNTLSLDGTAPNTM
                     NANIDLNGYNILNQGNPISITGFNWRGAWVTATAYVIGDVVEDGGSSYICIVDHTSGT
                     FATDLAAVKWELVSGAASGLPTQTGHAGKYLTTNGSSVSWEDASTLYATLAGATFTGG
                     ITGTTASFSGAVTISSGTITGITDLVVADGGTGASTHTANAVLIGEGTSPISSVSPGT
                     SGNLLTSNGTVWQSVAPATSGTAPTRQVFTASGTWTRPSGCTTVICRTQGGGGSGSSI
                     SSQYGGGGGGGGYAELLLDVSAISSVTVTVGTAGVASSFGAHCTGNAGGNASSYTPGS
                     GGGGSGGTVFTGSAGESSKASNGRYAAGGASHMGGGGAGNANGSLYGGGGGGAFGGGT
                     KSGAAGIVIVDEYY"
     gene            complement(1704..1970)
                     /locus_tag="AC159U_11080"
     CDS             complement(1704..1970)
                     /locus_tag="AC159U_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337634.1"
                     /translation="MAKDRKEKIKKLEKSIAGREKKLQEMDKSKFSKGDINLTPKKKS
                     KNLINPVLKSKKDGTMGGRARKAPLVNEESLRKLEKNKTRRRIA"
     gene            complement(1963..2244)
                     /locus_tag="AC159U_11085"
     CDS             complement(1963..2244)
                     /locus_tag="AC159U_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337635.1"
                     /translation="MPPVIKSGAKKGQRDYKRERANEKKNGDKRGKERAARNRARRAA
                     GLKVGDPREADHKKPLSEGGSTSQSNVQITSRKANANKEVARKKRKARG"
     gene            complement(2244..2447)
                     /locus_tag="AC159U_11090"
     CDS             complement(2244..2447)
                     /locus_tag="AC159U_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337636.1"
                     /translation="MAIDSRQKRFSMMSFGSPIRFYALNEADGTIDADDKYHLLNLYS
                     GITLDGAPEVSGLLTLPLMGAGQ"
     gene            complement(2447..4621)
                     /locus_tag="AC159U_11095"
     CDS             complement(2447..4621)
                     /locus_tag="AC159U_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR3337637.1"
                     /translation="MIVTAGKTNVSVYVYFVDDDGGTAPGEPTTGLLFSNIETGGSAS
                     YCRQGAARTDFTLVTLASASAAHTDGGFILVDDTNMPGLYRLDLPDAAFVTGVDQVIV
                     HLVAAAGNNTIMRPLAIDITNVDLRDAVRGGMTALPNANADAAGGLPISDLGGLDLDA
                     MNTNINDIETDTNELQTDWTNAGRLDAILDELTVNIDAVEADTQDLQTQVGAAGAGLT
                     GLPDVTIDATSVDLIWDENIVAAHTTADTAGKKLADAGGAADPWATALPGAYGAGTAG
                     NIIGNDVPAILVDTAEIGTAGAGLTSLASQTSVNTIDTNLDAVLVDTAEIGTAGAGLS
                     NITINDASVDKVWDETMAGHITVDTSGLVMNDLQDGGRLDLILDSRMAEASINTTGGA
                     VDNVTTVATTTTNTDMRGTDGANTTTPPTAAAIADAVWDEVITTGQHNQANSAGRLQR
                     LASDVVLIDSAVDTTVYGASTTTEFGTDLTQVDDFFNDTVIIFTSGALLGQSRTILNY
                     AQTNGVITLDEPLTSAPLNNVEFSLKATHVHPITQIVNSVLDELLSGHTTAGSLGKAI
                     TDIEADTNELQTDDVPTLISNLDAVVDTVKVDTAAILVDTNELQTDLTDGGRLDLILD
                     AVALEATVAALNDISTAQVNAEVVDVLKTDTVTLPAQGAPPVTPTMEQMVSWLYKVLR
                     NRTSQTATTWSLYNDAETVVDSKATVSDDGTTAIKQEIVSGP"
     gene            complement(4818..5801)
                     /locus_tag="AC159U_11100"
     CDS             complement(4818..5801)
                     /locus_tag="AC159U_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337638.1"
                     /translation="MSTDTIGTGKDHTTIADWYAAIPTTLTEDETGLLANQDFLQTST
                     LNLDGKTPGSWEIFLKPDTGASFRDSKDDAGFKLRYDTTQGCAISTTGSYVYPLILSD
                     VNITISELQISSSHTNTDGCLQNSADNNVMRDLIVYTTADNGVRAVSVLNGILVNSLV
                     IMNSDDAWQGAKCDYHGSIIQCTIVAPSDAAFPIATGAAKADSSDTVKSTALFECTTA
                     SEAGAWDAASDYNATDAATVVGGNSQNSLTLTNQFEGTTISTMDYRLKSGSDLIGAGN
                     IDASWPDDILGTVRATGTGGDCGCHEFVESATVIPNKIFQINQAVNRASYY"
     gene            complement(5798..6280)
                     /locus_tag="AC159U_11105"
     CDS             complement(5798..6280)
                     /locus_tag="AC159U_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337639.1"
                     /translation="MAIWQRVVKMVRLVVWVGDSEGTHDYGDVVSWRKEGDKNIGTKT
                     MQDPRFKVVHIPNMTEMQAISLESPQPGYGKIPQANPNLKYRAQTFNIAALTGATRAQ
                     VDLPHKVPSDKFRVEQDGKVTMLDVDNVRIDNLSEALAVVETKPIINVTDIGGDEVIG
                     "
     gene            complement(6255..6626)
                     /locus_tag="AC159U_11110"
     CDS             complement(6255..6626)
                     /locus_tag="AC159U_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337640.1"
                     /translation="MIINLGGNSNLFIRPFMFVKAGRSRPGHFHNFDHLTFVRHGSVR
                     IKFWKEIEGKEFVLGIHEYSAPVRESNDLRGGVVLIKKDVYHEITALEDWTHCACIYA
                     ARDLETGEVVDHYNGNMAACS"
     gene            complement(6628..7143)
                     /locus_tag="AC159U_11115"
     CDS             complement(6628..7143)
                     /locus_tag="AC159U_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337641.1"
                     /translation="MALYKIMNGASPTTVKFVAVTTGTAIKTLLQVKSSDTKPMRIVE
                     WGISFDGSAAATPIPTELLETGTIFGTVTAHVTAGIIPFDAEAKAAGGDVVTNLIPVG
                     TAATGYTATVEGAIVASRMFDNQLIAPTNQYVKQFPLGREPYVHTGSCLRIRTHAAAA
                     VNAVCYVIVEV"
     gene            complement(7152..>7524)
                     /locus_tag="AC159U_11120"
     CDS             complement(7152..>7524)
                     /locus_tag="AC159U_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337642.1"
                     /translation="IQTDTTTDIPALLPSALVGGRMDCSVGAVATDAISAAGVSAAAA
                     NKVSDHTWRRTVANVEASSDGDTIGTSRGAYVVLAKHNNKCGVSGGTLTVYQDDDITS
                     VITQTVTSDAAADPITDLDTV"
BASE COUNT         2040 a         1760 c         1335 g         2389 t
ORIGIN      
        1 cctgatactg atagagcagt ttattaactg ccactatcag gaagatcgca ttatccttaa
       61 attcttcagt agcgtcagga tgtatccaac gatccctgaa atattctcgg caagtaacta
      121 acattagtca gctttcttat cggaaaagaa tgcaccgata gcaccaatta atccaagacc
      181 accagcaaca atagctgtag tttgttcggg actaatagat agaccaactg ctgtaagtag
      241 cgcaactagt ccacgccatg tagatgcttc acttcctctt gctaatatcc atttaaacat
      301 atttatctcc ttaattaaat tttacgatct ttattgaaat ctactatggg aacagggata
      361 caaagccccc aacgattttc agcttcacct tctggacaag ctggtttctt ttcaccactt
      421 gcttcacatc ctactaaaaa taatcctgct aataatacta atgcgtattt catttgtttc
      481 tccttaataa tattcatcta ctataacgat ccctgctgct cctgatttag ttcccccgcc
      541 aaacgcacca ccaccgcctc caccatagag actaccattt gcgtttcctg caccaccacc
      601 ccccatatga gaggctccac ctgccgcata tcttccatta gaagctttag aagattcacc
      661 agcagaacca gtgaatacag ttccacctga tcctccgccg ccagatcccg gtgtataact
      721 agaggcattt cctcctgcat tacctgtaca atgtgcgcca aaactagaag caactcctgc
      781 agttcctact gtaactgtaa cagaactaat tgctgataca tccagtagaa gctctgcata
      841 acctcctcca ccgcctcctc ctccatattg agaggaaatg gatgatccag aaccaccacc
      901 accttgtgtt ctacagatta ctgtagtaca acctgaaggt cttgtccaag taccgcttgc
      961 tgtaaatact tgtcttgttg gtgcagtacc agatgttgct ggtgcaactg attgccaaac
     1021 tgtgccatta gaggttaata agtttccact tgttcccggc gaaacactag atattggaga
     1081 tgttccttca ccaattaata ccgcattagc tgtatgagtt gaagctcctg taccaccatc
     1141 tgccactact aaatctgtta ttcctgttat tgttccagat gatattgtta cagcgccaga
     1201 gaaactggct gtagttcctg taattccgcc agtaaatgta gctcctgcca atgtagcata
     1261 taatgtagaa gcatcttccc atgaaacaga agatccatta gttgttaagt attttcctgc
     1321 gtggcctgtt tgagtaggaa gacctgatgc tgctcctgaa actagttccc attttacggc
     1381 agctaaatct gttgcgaatg taccggaagt atgatctaca atacagatat aagaacttcc
     1441 accatcttca accacatctc ctattacata agctgttgct gtaacccatg cacctctcca
     1501 gttaaatcct gtaatactaa tggggtttcc ttgatttaat atgttgtagc catttaaatc
     1561 aatattggca ttcattgtat ttggagcagt tccatctaag gaaagagtat tatccagtgc
     1621 agcttctatt aatgcattat tagcgttata ggctgcggta gaaccatatc ctgctacaat
     1681 gtctgttagt gttagtttag ccattaggct attctccttc tggttttatt cttttcaagc
     1741 tttcttaaac tctcttcatt cacaagagga gcctttcttg cccttccacc catcgtacca
     1801 tctttcttac ttttaagtac tgggttaatt aaattctttg atttcttttt tggtgttaaa
     1861 ttaatatcac ctttactaaa tttagattta tccatttctt gtaatttctt ttctcttcca
     1921 gcaatggatt tctcaagttt tttaatcttc tcttttctgt ctttagccac gtgctttcct
     1981 ctttttacga gctacttctt tgtttgcgtt tgctttccgt gatgtaatct gcacattaga
     2041 ctgtgatgtt gatccgccct cactaagagg cttcttatgg tctgcttccc ttggatcgcc
     2101 aactttaagt ccggctgctc tccttgctct gtttctggca gctctttcct tacctctttt
     2161 atctccattt ttcttttcgt ttgcacgttc tcttttatag tcacgttggc ctttcttggc
     2221 tcctgattta attactggtg gcattattgt cctgctccca ttaatggtag tgttaatagt
     2281 ccggagactt caggcgcacc atctaaggta atgcctgaat atagatttag aagatgatat
     2341 ttatcgtcag catctattgt tccgtctgcc tcatttaaag cataaaatct gataggacta
     2401 ccaaaactca tcatactgaa tcttttttgt ctactatcta ttgccattat gggccactca
     2461 ctatttcttg tttaattgct gtagttccat catctgatac agtagcttta ctatctacaa
     2521 ctgtttcagc atcattatat aaagaccatg ttgtagctgt ttgtgaagta cgattccgca
     2581 atactttata aagccaactc accatttgtt ccattgtagg agtaactggt ggtgcaccct
     2641 gtgcaggaag tgttactgta tctgttttaa gaacatcaac cacctctgca ttaacctgtg
     2701 ctgtagagat atcatttaaa gctgctacag tggcttctag ggcaactgca tccagtatta
     2761 agtctaatct tccgccatca gttaaatcag tttgtaattc attagtatct actaatattg
     2821 ctgctgtatc taccttcact gtatcaacca cagcatctaa attacttatt agtgtaggta
     2881 catcgtcagt ctgtaattca tttgtgtctg cctcaatatc tgtaatggcc ttacctaatg
     2941 aacctgcagt agtatgtcct gatagtagtt cgtctaatac cgagttaact atctgtgtta
     3001 taggatgtac gtgtgtagct ttcaagctga actctacatt gttaagtggg gccgaggtta
     3061 aaggttcatc taaagtaata accccgtttg tttgagcata attaagaata gttcttgact
     3121 gtcccagtaa agctcctgaa gtgaagataa taacagtatc gttaaagaaa tcatctacct
     3181 gagtaaggtc agtaccaaac tcggtggtgg tagaagctcc atacactgtt gtatctacag
     3241 cggaatcaat caacaccaca tcggatgcaa gacgctgtaa tctacctgct gaattagctt
     3301 gattatgttg tcctgtagta atgacttcat cccatactgc atcagctata gctgctgctg
     3361 ttggtggggt tgtagtatta gcaccatccg ttccacgcat atcagtattg gttgtagtag
     3421 tagcaactgt ggttacatta tcaactgctc cacctgtagt attaattgag gcttctgcca
     3481 tacgggagtc taatattaaa tctagtctgc ctccgtcttg taaatcattc attaccagac
     3541 cagaggtatc gactgtaata tgtccagcca ttgtttcatc ccatacttta tctacactgg
     3601 catcattaat ggttatatta cttagtcctg cacctgcagt tcctatctcg gcagtatcta
     3661 ctaatactgc atctaaatta gtatctatag tatttactga tgtttgagaa gctaaactag
     3721 taagtccagc tccggctgta ccaatttctg ctgtatcgac aagtatagct ggtacatcat
     3781 tacctataat gttacctgcc gttccagcac catatgcacc cggaagggcg gttgcccaag
     3841 gatcggcagc cccacctgcg tctgcaagtt tcttacctgc tgtatctgct gtggtatgag
     3901 cagcaacaat gttttcatcc catataagat caacactagt tgcatctata gtaacatcag
     3961 gaagaccagt taaaccagct ccggcagctc ctacttgtgt ttgtaagtcc tgtgtatctg
     4021 cttctacagc atcaatatta acagttagtt catcaagtat agcatcaagt ctacctgcat
     4081 tagtccagtc tgtttgtagt tcatttgtgt ctgtttcaat atcattaata ttagtattca
     4141 tagcatctaa atctagtcca cctagatcag atattggtag tccaccagca gcatcagcat
     4201 tggcattagg taatgctgtc atcccaccac gtactgcatc acgtaaatct acatttgtta
     4261 tatcaatagc cagaggacgc atgattgtat tattgccagc agcagctact aaatgaacta
     4321 ttacctgatc tactcccgta acaaaagcag catcaggtaa atcaagtcta tataatcccg
     4381 gcatattagt atcatcaact aatataaaac caccatctgt atgagcagca ctagctgatg
     4441 ctaaagtaac caaagtaaaa tctgttcgtg cagccccttg tctacaataa gatgccgatc
     4501 ctcctgtctc aatgttagag aataaaagtc ccgtagttgg ctcgccgggg gctgttccac
     4561 cgtcatcatc tacaaagtag acatatacag atacatttgt ttttccagca gttactatca
     4621 taattctcca ttaaatctag gtgggtgtat taacaaaagt cgattcccgg cggggccgac
     4681 aggtactgca cccgtaatcc atactccatg catttgaaga aaacttacaa atccagatgg
     4741 tgttgatgct ggttgtaaat gaatattaaa cggctttatc agcggtttaa acggttgtga
     4801 tcttcctagt cgggccatta gtagtaactc gctctgttta ctgcctgatt aatttgaaat
     4861 attttattag ggataacagt agcagactct acaaattcat gacacccaca atccccacct
     4921 gtgcctgttg ccctcaccgt tcctaagata tcatcaggcc aagaagcatc tatattacca
     4981 gcacctatca aatctgaacc cgacttcaat cggtagtcca tagttgatat ggtcgtacct
     5041 tcaaactggt tggtcagagt aagagagttt tgactgttcc ccccgacaac tgttgcagca
     5101 tcagtggcgt tataatccga agcagcatcc catgcaccag cttccgaagc tgtagtacat
     5161 tcaaataacg ctgtagactt tacagtgtcg ctactatccg ctttcgctgc gcctgtagct
     5221 atcggaaatg cagcatccga aggggctaca atggtgcact gaataatact tccatgataa
     5281 tcacatttcg ctccctgcca tgcatcatct gagttcataa tcactaagct gttcactaat
     5341 ataccgttaa gaacactaac tgctcttaca ccattatctg cagtagtgta tacgataaga
     5401 tctcgcatca cattattatc tgcactgttc tgtaagcacc catctgtatt ggtgtggcta
     5461 gatgaaattt gtaattcgga tatggttata ttgacatcac taagaatcag tgggtataca
     5521 taagaacctg ttgttgaaat cgcgcaccct tgtgtcgtgt cataccttag cttaaatcca
     5581 gcgtcgtctt tactatctct aaaactagca ccggtatcgg gttttagaaa tatctcccat
     5641 gaccccggag ttttcccgtc aagatttaat gtgcttgttt gtagaaaatc ttggttggct
     5701 aataaacctg tttcatcttc ggtaagggta gtaggtatgg cagcgtacca atcagcaata
     5761 gtggtatggt ctttgcctgt tccaatagta tcggtactca tccaattacc tcatctccac
     5821 caatatctgt aacattaatg ataggcttgg tttcgacaac ggcgagagct tccgataggt
     5881 tgtctatcct aacattatca acatctaaca tagtgacctt tccgtcttgc tctactctga
     5941 atttatcaga tggaactttg tgaggcaagt ctacttgtgc ccttgtcgcg cccgttaaag
     6001 cggcgatgtt aaatgtttgt gcccggtatt ttaaattagg gttcgcttgt ggaattttgc
     6061 catatccggg ctggggtgat tctaatgata tagcttgcat ttccgtcata ttaggtatat
     6121 gtacaacttt aaaacgagga tcttgcatcg ttttagtgcc tatatttttg tcgccctctt
     6181 tccgccatga aactacatct ccgtaatcat gagtaccttc tgaatctcct acccatacga
     6241 caaggcgtac catcttaact acacgctgcc atattgccat tgtagtgatc tacaacttca
     6301 cccgtttcta aatcacgggc ggcatagata caggcacaat gtgtccaatc ttctaaagca
     6361 gtaatttcat ggtatacatc ttttttaatt aataccaccc cgcctcttaa atcattactt
     6421 tctcttaccg gagcagagta ttcatgtatt ccaagtacaa attctttacc ttctatttct
     6481 ttccaaaatt tgatacgtac cgatccatgc cgtacaaaag tcagatgatc gaaattatga
     6541 aaatgacccg gcctgctcct tccagctttt acaaacatga aaggtctgat aaataaatta
     6601 ctattaccac ctaagttaat tatcatatta aacctcgact attacataac atactgcgtt
     6661 taccgcagca gcagcatgag tgcgtattct taaacagctt ccggtatgaa catacggctc
     6721 acgccctaac ggaaattgtt taacatactg attagtagga gcaatcaact gattgtcaaa
     6781 cattcgtgag gctacaatag caccttcaac agtagccgta taaccagttg cagccgtacc
     6841 gacaggtatt aagttagtta ccacatcacc accagcagct ttcgcttcag catcaaaagg
     6901 aattatcccg gcagttacat gagccgttac cgtaccaaag attgttccgg tttccaacag
     6961 ctccgttggg ataggtgtag cagcagcaga accgtcaaag ctaattcccc actctacaat
     7021 cctcatgggt tttgtatctg aagatttcac ttgtaacaaa gtcttaatag ctgtaccggt
     7081 agtaaccgct acaaacttaa ccgtagtagg cgaagctccg ttcattattt tatataaagc
     7141 catatttttc cttatactgt gtctaaatca gtgatcggat cagcagcagc atcacttgtc
     7201 actgtttggg taataactga ggttatatcg tcatcctgat aaacagttaa tgttccaccg
     7261 gatacaccac atttattgtt atgttttgct aaaactacat aagcacccct gcttgtgcca
     7321 atagtgtcac catcactaga tgcttctaca ttagccactg ttctacgcca tgtatgatct
     7381 gaaaccttat ttgcggcagc agccgataca ccagcagcac ttatagcatc ggtagctaca
     7441 gcaccaacac tacaatccat acgtccacca actaaagcag atggtaataa tgcaggaata
     7501 tccgtagtgg tatcagtctg tata
//
LOCUS       DBNGSV010000306         3008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_817115, whole genome shotgun sequence.
ACCESSION   DBNGSV010000306 DBNGSV010000000
VERSION     DBNGSV010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_817115"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC159U_11125"
     CDS             complement(<1..327)
                     /locus_tag="AC159U_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337643.1"
                     /translation="MDKKDTFKLTNLITVLALLILLSSFSLYACKPEQKIEPPEPLAE
                     KYLNYIKNGEIEKATALYSDKMFSVVPKENWLKSLTVYRYFYGNLKSYELQHTKQVKN
                     DSKEFGG"
     gene            complement(405..755)
                     /locus_tag="AC159U_11130"
     CDS             complement(405..755)
                     /locus_tag="AC159U_11130"
                     /inference="COORDINATES: protein motif:HMM:NF039496.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR3337644.1"
                     /translation="MSSEKEQVERYYRGVPEQRLKKLRHISNIITQNYPDIEVSMKYK
                     MPTFNLDKNWVAIANQKNYISVYTCSYDHISDFKKKYPKIKTGKGCINFRDMDKIPID
                     DIKALIDKALSMKK"
     gene            complement(745..1797)
                     /locus_tag="AC159U_11135"
     CDS             complement(745..1797)
                     /locus_tag="AC159U_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HIR3337645.1"
                     /translation="MMFYNNIDMRFKCLYKILILFLLIIPALSCRSDAAVDPKFKVVV
                     LGASGGLNEQDLTSYLVAPENDNNYVALDAGTIYYGIKKAVELGSFKDIQVPDNSLLK
                     FESYILRHHIKAYLISHAHLDHTSGLIINSVADSTKTIIGIPKTIEYIRDHIFNWEIW
                     PNFGTEGAGHKINQYKYLRALPVKEIVIPETAMSVTSFVLSHGNDHYPSTAFLISSGE
                     YSVLYFGDTGPDVVENADNMKRVWEYVAPMVREKKLRAIFLESSFSNGRPDHMLYGHM
                     TPKWMMHELNVLAKLANKDTPGQALKGLPVVVTHIKPTIRAGIDPKEEIRKELDELNN
                     LGVDFIIPVQGQRYEF"
     gene            complement(1794..2987)
                     /locus_tag="AC159U_11140"
     CDS             complement(1794..2987)
                     /locus_tag="AC159U_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3337646.1"
                     /translation="MNNRPYWILPVLIFSQFAGTSLWFAGNAIISDLQLGWNLDPASI
                     GWVTSSVQFGFITGTLCFAFFAISDVFSPRKVFFVCSIVGALANLNIYLFADGLSTLL
                     VFRFLTGFFLAGIYPVGMKIASGWYKEGLGQAMGFLVGALVLGTAFPHLIKGLGETLH
                     WNSVIIAVSILCVLGGLLMFLLVPDGPNLYKGTRFNYKAIYHIFQSKDLRAASFGYFG
                     HMWEIYAMWAFIPVLLTIYSNLHGTEINIPIWSFIFIGCGSIGCSLGGILSKKIGSAS
                     VAFYQLTVSGVCCLFSPLLINAPESIFFLVLIIWGITIAGDSPQFSTLTAQTAPKEYV
                     GSALTIVTCIGFFITIFSIELVNFILTVFDAKYVFILLTPGPVLGLYYFIPLYKKRLY
                     EVANK"
BASE COUNT          972 a          606 c          505 g          925 t
ORIGIN      
        1 gccgccaaac tccttggaat catttttaac ctgctttgta tgctgaagct cgtatgattt
       61 taaattccca tagaagtaac gataaaccgt aagcgactta agccagtttt ctttaggtac
      121 tacactgaac atcttatctg agtaaagagc tgtagccttt tcaatctcgc cgttttttat
      181 ataattaaga tacttctcag ctagaggctc cggaggctct attttttgct caggtttaca
      241 cgcatagagg ctaaaactgc taagtaatat taataatgct agtacagtaa ttaaattagt
      301 tagtttaaag gtatcttttt tatccataat ttttgtatta tacatgaata ttttttcagt
      361 aagtacattt tgtaattatt gacttaactt aatgcctttg gaaattattt tttcatagac
      421 aatgctttgt ctatcaaagc tttaatatca tctattggaa tcttgtccat atctctaaaa
      481 tttatacagc ccttacccgt cttaatcttg ggatacttct ttttaaaatc agagatatga
      541 tcatagctgc atgtgtagac tgaaatatag tttttctgat ttgcaattgc cacccagttt
      601 ttatcaagat taaaagtagg catcttgtat ttcatactaa cttcaatgtc gggataattt
      661 tgtgtaatga tattgctaat atggcgaagc tttttgagtc tttgctccgg gactcctcta
      721 taatatctct ctacctgttc tttttcagaa ctcatatctt tgtccctgaa caggtattat
      781 aaaatccact cccaggttgt ttaattcatc aagttctttt cttatttcct ctttgggatc
      841 aatacccgct ctgatggtag gtttaatatg agttacaact acgggtaaac cttttaatgc
      901 ctgacctgga gtatctttat ttgccaattt agccaataca ttcaattcgt gcatcatcca
      961 cttcggggtc atgtgtccat atagcatatg atcgggacga ccatttgaaa aagatgattc
     1021 taaaaatatt gctctaagtt tcttttctct taccataggc gccacatatt cccagacacg
     1081 cttcatgtta tccgcattct ctaccacatc aggacccgta tcaccaaaat acaaaacgct
     1141 atattcaccc gagcttataa gaaatgcagt tgaaggataa tgatcgtttc cgtggcttag
     1201 cacaaatgat gttacactca ttgcagtttc cgggataacg atttctttaa ctggtagagc
     1261 ccttaaatac ttatactgat taattttgtg tcctgcgcct tcagttccaa aatttggcca
     1321 tatctcccag ttaaatatat ggtctcttat atactcaata gttttaggga ttcctatgat
     1381 agtttttgta ctatctgcga ctgagttaat tatcaaacca gatgtatgat ccaaatgcgc
     1441 atgggaaatc agatatgctt ttatatggtg tcttaagatg taggattcaa atttcaataa
     1501 tgaattatca ggaacttgaa tatccttaaa actaccaagc tccacagctt tttttattcc
     1561 ataataaatc gtgccggcat ctaaagctac atagttatta tcattctccg gggcaacgag
     1621 gtaagatgtc aggtcttgtt cattaagacc cccactggca ccaagtacaa ctactttaaa
     1681 tttaggatca accgcagcat cactacgaca actaagcgct ggaattatta aaagaaataa
     1741 tatcaatatt ttataaaggc atttaaatct catatcaata ttattgtaga acatcattta
     1801 tttgcaacct catataggcg tttcttatac aagggaatga aataatacag ccccaataca
     1861 gggcctggag taaggagaat aaacacatac tttgcatcaa acacggttag aataaagttt
     1921 actaactcaa tactaaatat ggttataaaa aaccctatac aggttacaat cgtaagtgcc
     1981 gagcccacat actcttttgg cgccgtctgt gcagtgagag tagaaaactg cggagaatca
     2041 cccgctattg ttattcccca tatgattagt actaaaaaga atatagattc cggtgcgttt
     2101 attaacagag gcgaaaatag acaacaaacc cctgacacag tcaattgata aaatgccaca
     2161 gaagcgcttc caattttttt tgataatatt ccccctaaag agcagcctat cgaaccacac
     2221 cctatgaata taaaagacca aatcgggata ttaatttcag tcccatgtaa attagaataa
     2281 atggtaagta gaacaggaat aaaggcccac atcgcatata tctcccacat atgaccaaaa
     2341 tagccaaatg atgcggcgcg caggtctttt gactggaaga tatgataaat agccttgtaa
     2401 ttaaatcttg tgcctttata tagatttggc ccatcaggta ctaataaaaa cataagaagt
     2461 ccgccaagta cgcataaaat ggatactgcg ataattacac tgttccaatg aagtgtctca
     2521 cctagcccct ttatcaaatg tggaaatgca gtgccaagca caagcgctcc gactaaaaat
     2581 cccatagcct gcccaagacc ttccttgtac cagccagatg ctattttcat accaaccggg
     2641 taaataccgg ccagaaaaaa cccggttaag aacctgaaaa caagtagggt agataatccg
     2701 tctgcaaaca gatagatatt aaggttagct aaagcaccaa caatggagca gacaaaaaac
     2761 actttccttg gagagaatac atcggagatt gcaaaaaaag cgaagcatag cgtgcctgtg
     2821 ataaatccaa attgcaccga agaagttacc cagcctatcg aagctggatc aagattccac
     2881 ccaagctgta gatctgaaat tattgcatta cccgcaaacc acagggaagt gcctgcaaac
     2941 tgggaaaaga tcagaaccgg aagtatccaa tatggtctgt tattcaaaat ttataagtga
     3001 taataata
//
LOCUS       DBNGSV010000307         2035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1370283, whole genome shotgun sequence.
ACCESSION   DBNGSV010000307 DBNGSV010000000
VERSION     DBNGSV010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1370283"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..751
                     /locus_tag="AC159U_11145"
     CDS             461..751
                     /locus_tag="AC159U_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337647.1"
                     /translation="MNKLLSVMVLTLSLGLSSLSFADTTVFGVKTPIVKKEVKDAIPG
                     GYVAKNLQDTFHVQKLKNQNITIARVDDIKDKFTGFRPSLHDRYSFEWYGKI"
     gene            complement(972..1424)
                     /locus_tag="AC159U_11150"
     CDS             complement(972..1424)
                     /locus_tag="AC159U_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3337648.1"
                     /translation="MNCVSTILSNKGAQVWTIHENETVYKGLEVMAEKDIGALVVLDS
                     LDRPVGIFSERDYARKVRLKGKSSLSTSVKELMVTELHTVSKDDSIEHCMTLMTENRT
                     RYLVVMELDKLAGLISTGDVVNQLLSHQKFQIKELTSYITGVPGKGQD"
     gene            1826..2029
                     /locus_tag="AC159U_11155"
     CDS             1826..2029
                     /locus_tag="AC159U_11155"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR3337649.1"
                     /translation="MAVARNTEIVGGSPKSWDDAVKQALKRANKTLRGLTGIELTKMN
                     AHIENGKITEYRAHLKITFILEE"
BASE COUNT          675 a          373 c          379 g          608 t
ORIGIN      
        1 gagtgaaatg ggcttacaga aaccatagag gactggctaa acacaagaga attaattact
       61 tcaaatggta caccttttta aagcaaatct gactcaaatt agcacacatt ttttgcaatt
      121 atgcccattt tgaatgtaca tgagaaatct gcttttaagc agcactgggt caagcacttt
      181 aacagcagat ctctctttga atttaaaagc ttacgaactt caccttaact ctgatttaaa
      241 gatggcaaac ttcagcttaa cgctaaagtc tgctgttgag atgacatatt tgagaaaata
      301 aataaccaac acactgagcc cagttccttt gactctgctc ggggcaggag aagtctgagt
      361 taagataaac taaccacgac tgtaatttaa taaaaatacg catcttttag tataggtaat
      421 tgtctaaata tacaaagata aaattaagga ggtaactata atgaacaagt tactgtcagt
      481 tatggtttta acattatcac tgggactaag ctccttaagc tttgccgata ctaccgtatt
      541 tggcgttaaa acacctattg taaaaaagga agttaaggac gctattccag gcggttatgt
      601 tgcaaaaaat ttacaggata cttttcatgt acaaaagctg aaaaatcaaa atattacaat
      661 agccagagtg gatgacatta aagacaaatt cacgggattc agaccttcac tgcatgacag
      721 gtatagcttt gaatggtatg gaaaaatata gaatatacgg aaagtaaaat cctgtaatta
      781 agttaattaa ctaaaagtaa ttatataata tagtcacttc aaaaatattt ggagtgactt
      841 ttattgtttg atacaaaatt ggttctctct attaccatta taaagttaaa aagtacctaa
      901 acggccggga ttatgccgct gtttacgttc cgtatgacca atatatagtg cgtttgttat
      961 ctatatatat atcagtcctg gcctttgccg ggaacgcccg ttatatagct tgtaagctct
     1021 ttgatctgga atttctgatg agacagcagc tgatttacaa cgtctccagt agatatgaga
     1081 cctgcaagtt tatcaagctc cataaccacc aggtatctgg ttctgttctc tgtcataaga
     1141 gtcatgcagt gttcgatgga gtcgtctttg ctaaccgtat gcagctctgt aaccatgagc
     1201 tcctttacag atgtgctgag cgatgatttt ccttttagcc tcacctttct ggcgtagtct
     1261 ctctcagaaa atatcccaac gggtctatcc aggctgtcca ggacaactaa tgccccgata
     1321 tccttttcag ccataacttc aagaccctta taaaccgttt cgttttcgtg tattgtccag
     1381 acctgagcgc ccttgtttga caatattgta cttacacagt tcatactaat agtatagcaa
     1441 atttagattt aaaatgcttg aatattttcc caagatattc aaaaatatca tttcaacaaa
     1501 ctcccacaac tgtcatcacc ccaacacatc tgtcattctt gaggcaaagc cgaagaatct
     1561 catgagactc ttcgctgtgc tcagagtgac aagtgtaaaa atttacaaag tcatcaaaag
     1621 gtcaaaaggt caatttttaa gggtatagcc cctgaccttt tgaacttttg accttttggg
     1681 gtggatgggg agttgaaaac atgtatggct tatcacgatt gggagtgaga cagattcggg
     1741 atgttgctct aaaaaaagat cgctttacat caaaataatg tgtatgctaa taaattacta
     1801 tcaatataat caaggaggtt caattatggc agttgcaaga aataccgaaa tagtgggtgg
     1861 ttccccaaag tcatgggatg atgctgtaaa gcaggcttta aaaagagcca ataaaacatt
     1921 aagaggttta accgggattg agttaacgaa aatgaatgcc catatcgaaa acggaaagat
     1981 taccgaatac agggcacatt tgaaaataac ttttattctt gaagaatagg taata
//
LOCUS       DBNGSV010000308         3546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_679633, whole genome shotgun sequence.
ACCESSION   DBNGSV010000308 DBNGSV010000000
VERSION     DBNGSV010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_679633"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1339
                     /locus_tag="AC159U_11160"
     CDS             131..1339
                     /locus_tag="AC159U_11160"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR3337650.1"
                     /translation="MPICSVEEAIEEIQNGRMVILVDDEDRENEGDIVVAAEFITPEI
                     INFMAKFGRGLICLALTNDNADRIGLQPIKPENNPVPQYTAFTISIDAKNETTTGISA
                     SDRASTITKAISEDSTAHDFMRPGHVFPIISRKGGVLVRAGHTEGSVDLAKLAGLTPA
                     SVICEIMKDDGDMARLNDLEEFSKEHDVKITTIANIISHRLRSECLVSKAANANLPTQ
                     YGDFKVIVYENGIDSQHHVAFVKGDITPDEGVLVRVHSECLTSDVFGSLRCDCGPQIH
                     QAMRMIEAEGRGVILYMRQEGRGIGLINKIKAYALQDDGYDTVQANEKLGFKPDLRDY
                     GIGAQILLDLGVRKMKLMTNNPQKVKGLEGFGLQIVERVQIEIPPNEANTNYLKVKKD
                     KLGHLLSMVD"
     gene            1357..1824
                     /gene="ribH"
                     /locus_tag="AC159U_11165"
     CDS             1357..1824
                     /gene="ribH"
                     /locus_tag="AC159U_11165"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3337651.1"
                     /translation="MTKVYEGVLNAKGLKFAIITSRFNSFITQQLVEGAKDTLIRHGC
                     SENSIELVKVPGAYEISFAVKKILETKKYDAVIAVGAIIRGETPHYDYLSSEVTKRLV
                     DLSIQHDIPVASGIITTENLEQAIERAGSKAGNRGSEAAISAIEMANLVNSLS"
     gene            1859..2308
                     /gene="nusB"
                     /locus_tag="AC159U_11170"
     CDS             1859..2308
                     /gene="nusB"
                     /locus_tag="AC159U_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006836875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3337652.1"
                     /translation="MGLRRRAREHSLQFLYQVDLLKSSYNKSDILQQIELYWNNKDKT
                     PDKELVEFSNRINIGVFENLDTLDDIISTNCKNWKFSRLSSIDRNILRIAVYELLFMK
                     DVPSKVVINEAIEIAKKFGTEDSGSFINGILDHVNKAVSKGVKNDNK"
     gene            2295..3317
                     /gene="trpD"
                     /locus_tag="AC159U_11175"
     CDS             2295..3317
                     /gene="trpD"
                     /locus_tag="AC159U_11175"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3337653.1"
                     /translation="MTINEGIAKVVERVDLTEEEMISVMDKIMEGSVSDSQIGAFLVG
                     LRMKGESIGEITGAAKVMRSKSRKIKSDRPVVVDLCGTGGDMQGTFNISTVASFITVG
                     AGVAVAKHGNRSVSSKVGSADVLEKLGVNININSQGAEKCLNETGFTFLFAPNYHPAM
                     KNVGKPRKEMGIRTIFNILGPLTNPVSLKHQIMGVYSQSLIEPLAKVFRNLDYERAMV
                     VHGADSLDEFSVTGKTDVAELNEGIVKTYQVDPQQLGLKTWSLDELKGDDLEQNAKIA
                     LSILNGQEQGAKREISILNSAASILVANKAADFKEAIEKARESIDSGNAIKALEKVIK
                     FTTTWN"
     gene            3346..>3546
                     /locus_tag="AC159U_11180"
     CDS             3346..>3546
                     /locus_tag="AC159U_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR3337654.1"
                     /translation="MILDEIIENKILEVEQSKKDTPLDKLLADLETCPEPKDFYSALN
                     SREGEIRIIAEIKQASPSKGVLT"
BASE COUNT         1238 a          595 c          772 g          941 t
ORIGIN      
        1 gcatgattat atatgtaggg attaaagagt attaaaaatt atacgcctaa tacggctgtt
       61 attgacaaag caagagcttt acatattatt taggcgtttt gagaacaaaa tacaatatta
      121 ggaagtaatt atgccaattt gcagtgtaga agaagcaatc gaagagatac aaaatggaag
      181 aatggtcatc cttgtagatg atgaggacag agaaaatgag ggtgatattg tagtagctgc
      241 cgaatttatt acgcctgaaa tcattaattt tatggctaag tttggaaggg gtcttatttg
      301 tcttgcctta acaaatgata atgccgacag gataggccta cagcctatta aaccagagaa
      361 taatcctgta cctcaataca ctgcatttac aatatctata gatgccaaga acgaaaccac
      421 aacaggaatt tctgcctcag acagagcctc tacaataaca aaagcaattt ctgaagactc
      481 caccgcacat gatttcatga gaccaggcca tgtatttcca ataatttcga gaaagggcgg
      541 tgtgctggtt agagccggtc atacagaagg gtcggtagac cttgcaaaac ttgcagggct
      601 tacccctgca tcagtcatat gtgagataat gaaagacgat ggagatatgg cacgcctaaa
      661 tgatcttgag gagttttcaa aagaacacga cgtcaaaatt acgactattg ccaacattat
      721 tagccataga ctgcgctctg agtgtctggt cagtaaagct gcaaacgcca atctgccaac
      781 ccagtatggt gattttaagg ttatagttta tgaaaatggt attgactcac agcaccatgt
      841 agcttttgta aaaggtgata tcactcctga tgaaggggtg ctcgtaaggg ttcattccga
      901 gtgtcttaca agtgatgtat tcggatcact taggtgtgac tgcgggccgc aaatacatca
      961 ggccatgaga atgatcgaag cagagggcag aggtgttatt ttgtatatga gacaggaagg
     1021 aaggggaatt ggcttaataa ataaaataaa agcctatgcg ctgcaagatg acggttacga
     1081 tactgtacag gcaaatgaga agcttgggtt taaaccggat ttaagagatt atggaatagg
     1141 tgcacaaatt ctccttgatc ttggtgtaag aaaaatgaaa ctgatgacca ataatcctca
     1201 aaaagttaaa ggactcgaag gatttggtct gcagattgta gaaagagttc agatagaaat
     1261 acctccaaat gaggcaaaca ctaactattt aaaagtaaaa aaagataaac tggggcattt
     1321 actgtccatg gttgattaac ataaaagagg gtcattatga caaaggtata tgagggtgtt
     1381 ttgaacgcta agggactcaa gtttgcgatt atcacaagca gatttaacag ttttattacc
     1441 cagcagcttg ttgaaggagc taaggatacc cttataaggc atggctgttc agaaaacagt
     1501 attgaactgg ttaaggtacc cggagcatat gagatatcat ttgcagtaaa aaaaatactt
     1561 gaaacaaaaa aatacgatgc cgtgatagct gttggcgcta ttattagagg cgaaactccc
     1621 cattatgatt acctctcatc agaagttacc aaaaggcttg ttgaccttag cattcaacat
     1681 gatattcctg tagcctcagg aattattact actgaaaact tggaacaggc aatagaaaga
     1741 gcagggtcta aggcaggcaa ccgtggatca gaagctgcaa tctctgcaat tgaaatggca
     1801 aatcttgtaa attctctttc ttaaaaatta ttaatcatca aacttcagtg atataaagat
     1861 gggtttaagg cggagagcaa gagaacattc acttcagttt ttatatcagg ttgatcttct
     1921 aaaatcttca tataataaat ccgatatact gcaacagata gaactttact ggaacaataa
     1981 agacaaaacg ccggataaag agttagtcga attttcaaac agaataaata taggtgtttt
     2041 tgaaaatctc gacacactcg atgatatcat ctcaacaaat tgcaagaact ggaaattcag
     2101 caggttatca agtattgata gaaacatcct taggattgca gtctatgaac tgctgtttat
     2161 gaaagatgta ccaagcaaag tagtaattaa tgaagctata gagatagcaa aaaaatttgg
     2221 cactgaagat tcaggatcat ttataaatgg tatattagat catgttaaca aagcggtaag
     2281 taaaggggtt aaaaatgaca ataaatgaag gtattgcaaa agtagttgaa agggttgatc
     2341 ttactgagga agaaatgata agcgttatgg ataagatcat ggaaggatct gtttctgata
     2401 gtcagatcgg cgcttttcta gtcggcctta gaatgaaagg tgaatctatt ggtgaaatta
     2461 cgggtgcggc taaagttatg agatcaaaat cgagaaaaat aaagtctgac cgccctgttg
     2521 tagtcgacct ttgcggcacg ggtggagaca tgcaggggac ctttaacata tcaactgtag
     2581 cttcctttat aaccgttggt gccggtgttg cagtagccaa gcatggcaac agatcagtct
     2641 cgagtaaagt gggaagcgct gatgtcctgg aaaaacttgg tgttaacata aatattaatt
     2701 ctcagggtgc agaaaaatgt cttaacgaga cagggtttac gttcttattc gctcccaatt
     2761 accatccggc catgaaaaac gttggcaagc caagaaaaga gatgggaatc agaactatat
     2821 tcaatatttt aggccctctt acaaacccag tgagccttaa gcatcagata atgggtgtct
     2881 actctcagtc tctcattgaa cctcttgcca aagtgtttag aaatcttgac tatgaaagag
     2941 ccatggttgt tcacggagct gattccctgg atgaattttc agtaaccgga aaaacagatg
     3001 ttgccgagtt aaacgaagga atagtaaaga catatcaggt tgatccgcag caactcggtc
     3061 ttaaaacatg gagtcttgat gagctaaagg gtgatgacct ggaacagaac gcaaaaattg
     3121 ctctttccat actaaacggg caagaacagg gagcaaaaag agaaatatcc attttaaatt
     3181 ctgctgcatc aatacttgta gcaaacaaag ccgcagattt taaagaagcc attgaaaaag
     3241 cccgggagtc tatcgacagc ggaaatgcta ttaaggcatt agaaaaagta ataaagttta
     3301 ctactacatg gaactaatat taagtttaat tagctttaat ttaaaatgat tcttgatgaa
     3361 ataattgaaa ataaaatact tgaagtagag cagtccaaaa aagatactcc gcttgataaa
     3421 cttcttgcag acttagaaac ctgccccgaa cctaaagact tttacagtgc attaaactcc
     3481 agagagggag aaatacgtat tatagcagag atcaaacagg catctccatc taaaggggta
     3541 ttaacc
//
LOCUS       DBNGSV010000309         2525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_471202, whole genome shotgun sequence.
ACCESSION   DBNGSV010000309 DBNGSV010000000
VERSION     DBNGSV010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_471202"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..479)
                     /locus_tag="AC159U_11185"
     CDS             complement(138..479)
                     /locus_tag="AC159U_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014417968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337655.1"
                     /translation="MCNECDKRGKTWEGSDPTCAFLLDEFHTDNWNCATMNKLRNICY
                     ENGFHNRDDNCNASIALLNIPENDYVYGYLIMTWYKSRGRVGKAVIMCDDEDPHILKL
                     KEAEAIIECFI"
     gene            551..730
                     /locus_tag="AC159U_11190"
     CDS             551..730
                     /locus_tag="AC159U_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337656.1"
                     /translation="MNNILTKTELENIYRENSNDDAATIIGVSVPTMLKYLKDNDIKL
                     KGAGANNQKKVQIVD"
     gene            739..1224
                     /locus_tag="AC159U_11195"
     CDS             739..1224
                     /locus_tag="AC159U_11195"
                     /inference="COORDINATES: protein motif:HMM:NF018785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HIR3337657.1"
                     /translation="MDTDIWFPFYYGDYLKDTMQLSAERHGIYLLLLIHCWQNGYIEN
                     DIDSISMITKVSVDSKSLLYILDKYFVKKDNKYYQNRITKELKCAKENKEKNKQRAIN
                     AANARWKNNATSNATSNATSNATSMLQLCPSPSPSQGEEGEESFSTEVEDSIKMWNRN
                     Q"
     gene            1206..2468
                     /locus_tag="AC159U_11200"
     CDS             1206..2468
                     /locus_tag="AC159U_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337658.1"
                     /translation="MEQKPMSETTTKKNEVIKYLNSNIPENPESLYQILYTFKFEEID
                     IKDVLDIFCNFYDNTPEVSQQMLLEYREYYRNKKTIEHLEEKEQERIDMLEELRLEMS
                     NVSANPEQFKKLDLENYVSLWTQKVKPSAPIPKVINFMDCKPVEVEIILRVGFIQIPK
                     GITCCIHAPGGAGKSILMQRVIMASGIRACYISGEDSPGLMANLLQPYKRFYSNNNAD
                     FVHFDNQDIGGVLEFIEYSDYELYVVDPLSSFCTGDKWGRVETENGVASELLRIMNGI
                     TAKTRKTIIYTHHEAKSTNDIRTAARGSSALVDNARMGISINRLSERYRKPHSAKKTK
                     YQEQEEHTSNVKCFQRALTRELSKQPQSVFKNQDMIGVVEMVCHKNNYGSVGDMAQFI
                     LTANIGRPGLNVVPAGGLQIELREAISD"
BASE COUNT          983 a          361 c          429 g          752 t
ORIGIN      
        1 agccataatt aaaccaactg atttatcagg aagcattttc ataaactcca aacaatccat
       61 attaaaagcc tggtttaatt ctatctcttt accgttagtt aatgttattg ttttcactgt
      121 ctctcctatt aaatccatta tataaaacat tcaattattg cttcagcttc ttttagtttt
      181 aatatgtgag gatcttcatc atcgcacata atgacagcct ttccgactct acctcttgat
      241 ttataccatg tcataattaa atatccataa acataatcat tttcaggaat attcaacaac
      301 gcaatagatg cattacaatt atcatctcta ttatgaaatc cattttcata acaaatattt
      361 ctgagtttat tcattgttgc acaattccaa ttatctgtat gaaattcatc taataaaaat
      421 gcacatgtcg ggtctgatcc ttcccatgtt ttccctcttt tatcgcattc attacacata
      481 ttatttctcc tattgacttt attctatatt aatattaaaa taatgtaaag agtaaaagaa
      541 ggagaaataa atgaataaca ttttaacaaa aacagaatta gaaaacattt acagagaaaa
      601 cagtaatgat gacgcagcaa ctataattgg tgtttcagtt cctacaatgc ttaagtattt
      661 aaaagacaac gatattaaat tgaaaggagc aggcgcaaat aatcaaaaga aagtacaaat
      721 agttgattag gagtttttat ggatacagat atttggtttc cattttatta tggtgattac
      781 ttaaaagaca caatgcaatt atcagcagaa agacatggta tatatctttt attattaata
      841 cattgttggc aaaatggata tatagaaaat gatattgatt ctatttcaat gatcacaaaa
      901 gttagtgttg atagcaaatc tttgttatat atacttgata aatattttgt taagaaagac
      961 aataaatatt atcaaaacag gataacaaaa gaacttaaat gtgcaaaaga aaacaaagaa
     1021 aagaataaac aaagagctat aaatgctgca aatgcaagat ggaaaaataa tgctacaagc
     1081 aatgctacaa gcaatgctac aagcaatgct acatctatgc tacaattatg cccttcacct
     1141 tcaccttcac aaggtgaaga aggagaagaa tctttttcca ctgaagtgga agattctatt
     1201 aaaatgtgga acagaaacca atgagcgaaa ctacaacaaa aaagaatgaa gtaataaaat
     1261 atttaaacag taatattcct gaaaatccag aatcgttgta tcaaatcctt tataccttta
     1321 agtttgaaga gatagatata aaagacgtat tagatatatt ctgtaatttc tatgataaca
     1381 ctcctgaagt atcacagcaa atgctacttg aatacagaga gtactataga aataaaaaga
     1441 ctattgaaca cctagaagaa aaagagcagg aacgaataga catgctagaa gagttaagac
     1501 tagaaatgtc aaatgtgtct gctaatccag aacagtttaa aaagttggat ttagagaatt
     1561 atgtatcttt gtggactcaa aaagtaaaac cttcagcacc tatacctaaa gtaataaatt
     1621 ttatggattg taaacctgtt gaagtagaga taattttaag agttggattt atacagatcc
     1681 cgaaaggtat aacatgttgt attcatgctc caggaggtgc agggaaaagc attttaatgc
     1741 aaagggtaat tatggcaagc ggaattagag cttgttatat atccggggaa gatagtcccg
     1801 ggttaatggc taatctattg cagccttaca aaaggttcta ttcaaataat aatgctgact
     1861 ttgtacactt tgataatcag gacattggtg gagtgttaga gtttatagaa tattctgact
     1921 atgagcttta tgttgtcgat ccattatcaa gtttttgtac aggtgataag tggggaaggg
     1981 tagaaacaga aaacggtgtt gcatcagagc tattaagaat tatgaatggt attactgcta
     2041 agactaggaa aactataata tacactcacc acgaagcaaa aagcacaaat gatataagaa
     2101 cagcagccag aggatcaagt gcattagttg ataatgcacg aatgggaata tcaataaata
     2161 gattatcaga gagatataga aaaccacatt cagcaaagaa aactaaatat caagagcaag
     2221 aggagcatac atcaaatgta aaatgtttcc aaagagcatt gacaagagaa ttaagcaagc
     2281 agccgcaaag tgtatttaag aatcaagata tgataggagt ggtagaaatg gtctgtcata
     2341 aaaataatta tggaagtgtt ggagatatgg cacaatttat actaactgct aacattggaa
     2401 gaccaggatt aaatgttgtt cctgccggtg gattgcaaat agaattgaga gaagctattt
     2461 cagattaatt catgatacag gaatattaaa tttggtcgat gatatagcaa ggagaagaaa
     2521 caaat
//
LOCUS       DBNGSV010000310         3459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1302755, whole genome shotgun sequence.
ACCESSION   DBNGSV010000310 DBNGSV010000000
VERSION     DBNGSV010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1302755"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="AC159U_11205"
     CDS             <1..813
                     /locus_tag="AC159U_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337659.1"
                     /translation="MIPVEYFQTELLNSITSAVYSFYKYWFFNIGLSLITLTASFFIV
                     DRQRKYIFLIFLSLFILGSTFQFSIEMAGNHKIFNLWIVLANMYTAYLLYLVYSKFKL
                     GTIISILLLIPLTFSGIFDLIPIKNDGKVVLDDFQKQPMAAWVFNNTNQDDVFLTTYR
                     ILNPISLAGRRTTQGWPYFAWSTGYDTKGREDLVKDLYNPKSLKDFCEKSKLIHFDYV
                     QTELQFEKEPHFEINYRFFDDNFVPVFHDKTSRLEEKIYPIDNVCNNDNIKE"
     gene            934..1881
                     /locus_tag="AC159U_11210"
     CDS             934..1881
                     /locus_tag="AC159U_11210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3337660.1"
                     /translation="MKNLLSIVIPAYCEDRNIALIHKELTNVLKKIKTQYEIIFVDDG
                     SKDDTWDEILKLYNQDKHIKGLRFTKNFGHQYALLAGMNYAKGQAVITLDADLQHPPE
                     IITQLLQKWKEGFKIVNTQRIDNTKLSYFKKTSSTLFYKLFSFLSGVQLADGMADFRL
                     LDRKVIDELLKFNEAGLFIRGLIQWSGFPSINIQYTPNTRQIGESKYDLTKMIRLALS
                     AITSFSIIPLRLGIIIGIITSLLAFLELLFVIFTKMFSYDAVPGWASAVGVLSFLFGI
                     MFILIGLLGEYIGQIFMQVQNRPKYIINETAGYDKEDKQ"
     gene            complement(1925..>3459)
                     /locus_tag="AC159U_11215"
     CDS             complement(1925..>3459)
                     /locus_tag="AC159U_11215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3337661.1"
                     /translation="LLGIVLRTAGLDLFPFGLAGDEAWSGLEAQRILQQGGIGAWSPT
                     ALGQPSGYFYWVALFFTFLPNDIGSLRLTFALIGIITLVLCYLFTRSFFGRFVAIVSL
                     FLLCVSPWHILISRSALSYLFPPLFISASLMFMNRGLKSNNLLYLCFAGIAFGLGFHT
                     YLGYFASTYIFVLLWIVLIVKFRKNYDKILIDATVFWSFAILASLPVLIFIYNHTGDI
                     IWRSNFSSVFALAEYKNLNDSSSKILFFATQIYKSIASFFVTYNGVFSRTGILGLSTK
                     IFLLIGIIISFIRYKDWRYNVLLIGFIIAILQIALTTNTGALFPHVEKQAGTRGVIAL
                     PMIYALAGIGFAFTLQKLSRLFPLTLVWIPRVFMCIVLILYGAYYLNKSYKFSAQSDS
                     VKWFYEPSIRLTSEYLDELRKSEGDIYVYFYSDRWFWNYEPIRFLAPDLKGENRSRKF
                     GEFSIELNPAHSKVVYLLMPPYDEYLEQLKSKYPGGQQYQINYDNQLMFKGYYLGANN
                     LK"
BASE COUNT         1273 a          562 c          546 g         1078 t
ORIGIN      
        1 atgataccag tagagtattt ccaaaccgaa ttattaaatt ccataactag tgctgtttat
       61 tcattttata aatactggtt ttttaatatt ggtttgagtt taataacgtt aaccgcttct
      121 tttttcatcg ttgacagaca aaggaagtat atcttcctaa tatttctaag cctcttcatt
      181 cttggaagca catttcagtt tagtattgaa atggccggta atcacaaaat ttttaattta
      241 tggatagtat tagcaaatat gtatacagct tatttgcttt atcttgttta tagtaaattt
      301 aagctgggta caattatatc catactactt cttatcccac tcacattttc tgggattttt
      361 gatctcattc caataaaaaa tgatggcaaa gtagtactgg atgattttca aaagcaaccc
      421 atggctgcct gggtctttaa caacacaaac caagatgatg tatttcttac aacatataga
      481 attctaaatc ccatatcact tgcaggaaga agaacaaccc aaggctggcc atattttgcc
      541 tggagtacag ggtatgacac caagggaaga gaagatttgg taaaagatct atataacccc
      601 aaatcattaa aagacttctg tgaaaaatca aaattgattc attttgatta tgtacaaacc
      661 gaactacagt ttgaaaagga acctcatttt gaaataaact atagattctt tgatgataat
      721 tttgtaccgg tttttcatga taagacttcg agactcgaag aaaaaattta tccaatagat
      781 aatgtttgta ataatgataa tataaaagag tgataaacta caaatggaat agatctaaaa
      841 ttttatctat ttattatgca tttaaataca tttatttaat ataattggaa tataaatggt
      901 aaatattaat ataggtttat ttaaactaat aaagtgaaaa acttactatc aatagttatc
      961 cctgcttatt gtgaagacag aaacatagca ttgatacata aagagttaac aaatgtatta
     1021 aaaaaaataa aaactcaata tgaaataata ttcgtggatg atgggagcaa agatgataca
     1081 tgggatgaga ttctaaaatt atataatcag gataaacata taaaaggatt gagattcact
     1141 aagaacttcg gccatcagta tgctttactt gcaggcatga attatgcgaa gggtcaggca
     1201 gtaattacat tagatgcaga tctacaacat cctcccgaaa ttattactca attgttacaa
     1261 aaatggaaag aaggattcaa gatagtaaat acccagcgaa tagataatac caaattatct
     1321 tatttcaaga aaacatcatc aacattgttt tataaattgt tttcatttct aagtggagtt
     1381 caacttgcag atgggatggc agacttcaga cttttggaca gaaaagttat agacgaatta
     1441 ctaaaattca acgaggctgg tttgtttatt agaggattaa tccaatggag cggtttccca
     1501 tcaataaaca ttcaatatac acccaacaca agacagatcg gtgaatcaaa atatgaccta
     1561 acaaaaatga tacgtttagc cttgtccgct ataacttcat tctctataat tcctttgcgt
     1621 ttggggatta ttataggaat tatcaccagt ttgctggctt ttttagagct actatttgtt
     1681 atatttacaa agatgttttc gtatgatgca gtaccaggtt gggcatcagc agtaggagta
     1741 ttatcttttt tattcggcat tatgtttata ttaataggat tgctgggtga atatataggg
     1801 cagattttta tgcaagttca gaatagaccc aaatatataa taaatgaaac tgccggctat
     1861 gacaaagaag ataaacaata gcatgtaaac aacccattaa tattcaaagc actatatatt
     1921 tatctcactt taagttgtta gctccaagat aatatccttt aaacataagc tggttatcat
     1981 agttgatttg atactgttgt cctccgggat attttgattt taattgttcc aaatattcat
     2041 cgtaaggagg cataagtaaa tacactacct ttgaatgagc gggatttagc tctatagaaa
     2101 attccccaaa cttcctggaa cgattctccc ctttcaaatc cggtgcaaga aatctaattg
     2161 gttcataatt ccagaaccac cggtcggaat agaaataaac atatatatca ccttctgatt
     2221 ttcttagttc atctagatat tcagaagtta agcgtatcga aggctcataa aaccacttaa
     2281 ctgaatcact ttgagcagaa aatttataag atttattcaa ataataagca ccatataaaa
     2341 ttaatactat gcacatgaaa actcttggta tccaaaccaa tgtcaatgga aaaagtcttg
     2401 atagtttttg tagtgtaaac gcaaaaccaa taccggctaa tgcataaatc atgggtaatg
     2461 ctataacacc tcttgtaccc gcctgttttt ctacatgagg aaacaacgca ccagtatttg
     2521 ttgttaaagc aatctgtagt attgctataa taaatccaat taataataca ttgtaacgcc
     2581 agtctttgta tcggataaag cttataatta ttcctattaa taagaatatc tttgtagaca
     2641 atccaagaat gccggttcta ctaaagacac cattatatgt aacaaagaat gaagcaatag
     2701 acttatatat ttgtgtagca aaaaatagta ttttcgaact ggagtcatta aggtttttat
     2761 attcggcaag cgcaaataca gatgaaaaat ttgatctcca gataatatct cccgtatggt
     2821 tatagataaa aatcaatacg ggtaacgatg ctaaaattgc aaatgaccaa aatacagttg
     2881 catctatcaa aattttatca taattttttc tgaatttaac tataagaact atccaaagaa
     2941 gtacaaaaat atatgttgat gcaaaatatc ccaaatatgt atgaaatcct aaaccaaagg
     3001 caataccggc aaaacaaaga tataataaat tattactttt caaaccacga ttcatgaaca
     3061 ttaatgatgc agaaatgaat agtggtggaa ataaataact aagtgcagaa cggcttatta
     3121 atatgtgcca agggcttacg catagaagga ataaactaac aatcgcaaca aatcgcccga
     3181 agaagcttcg tgtaaacaaa taacacagaa caagagtgat tataccaatt aatgcaaatg
     3241 ttagccttaa actaccaata tcatttggga gaaatgtaaa aaataatgca acccaataaa
     3301 aatatcctga aggttgacca agagcagtag gagaccaggc tcctatacct ccttgctgta
     3361 agatacgttg tgcttcaagt cccgaccagg cttcatcccc tgctaatcca aatggaaata
     3421 gatccagacc tgctgtcctc agtactattc ccaataata
//
LOCUS       DBNGSV010000311         2274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_610042, whole genome shotgun sequence.
ACCESSION   DBNGSV010000311 DBNGSV010000000
VERSION     DBNGSV010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_610042"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..282
                     /locus_tag="AC159U_11220"
     CDS             76..282
                     /locus_tag="AC159U_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337662.1"
                     /translation="MTPDNNQSLVDRKQLIELYPALGAKKYRLDHLIRTRQIPLIRIG
                     KNIYYNPQDIDNWIKENQIKTGSK"
     gene            279..536
                     /locus_tag="AC159U_11225"
     CDS             279..536
                     /locus_tag="AC159U_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337663.1"
                     /translation="MMKKKQKRPEGAATPSSTHNFNSTTTISLPIIIKIGNSTIAEIK
                     QATVCINGLCDYHSKRIIKGHVLKEYLSELNIKGMEVVING"
     gene            529..>2274
                     /locus_tag="AC159U_11230"
     CDS             529..>2274
                     /locus_tag="AC159U_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013930.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR3337664.1"
                     /translation="MDNIFEHIKNQVPFETVANEYGINFKNKKAHCPFHEEKTPSFHN
                     YGTHGKCFGCEKSADAIEIEAHFKGLSPLQAAKSLAERYNINIETYLDNNHNSDLSDT
                     DIPNEKSQNKSQSEKLVDLILNDDIELFHDSDKTAYAQIKRGSHFEYYKIRGKDFRLW
                     LTGNYWDKYQKTTNSNSMAEALNIAEAKALFDGKEHKLFNRVAKSEITIMYDLGNWGG
                     VLIHESGWSILELLPSVFKSYPHQKIQVEPIKSELNELYRLFNYINIQEADYLLFLSD
                     LTFAYVADLPHPILALMGDFGTAKTTAAKVIKSLVDPSELEVLTPPSTKQELAQLASH
                     HWVLPFDNLHTIPQWFSDSLCKIVSGDGFSKRELYSDDNDIFYSYKRKCILTSINQML
                     SSPDVLSRSIIFQLEPIHKDKRKEESVFWKDFNREKPMLLGAIFSLISKTLANLDNVN
                     HKPFSRLADFEKWGCAVALTLGREEEDFISEYKNNIELQNRESIESSPVAKTLLSYME
                     FKEYWEGSPSELLNELNPTAEELKYYSDRKYPKDPRWLWRRLKEVSTDLYTYGIEIKH
                     EDTCHGERAISITNNN"
BASE COUNT          832 a          437 c          408 g          597 t
ORIGIN      
        1 tatttgtaat ctgtatacat tctatctcat gattataatt atcacaaatc taataaacac
       61 aaggaggtca tacatatgac accagataac aaccaaagtc ttgtagatag aaaacagtta
      121 attgagcttt atcccgccct cggtgctaaa aagtacaggt tagatcacct tatacgcaca
      181 cgccaaatcc ctttaatccg tatcggtaaa aatatttact acaatcctca ggatatagat
      241 aactggatca aagaaaatca aatcaaaaca ggaagtaaat gatgaagaaa aaacaaaaaa
      301 ggcccgaagg tgcggcaaca ccctcgagca ctcataactt taattctaca actacaataa
      361 gtttaccaat tattatcaaa atcggcaact caacaattgc cgagataaaa caagccacag
      421 tctgtataaa cggcctgtgt gactaccatt ccaagcggat tattaaaggt catgtattaa
      481 aggaatacct ctcggaactg aatattaaag gaatggaggt tgtcattaat ggataatatt
      541 tttgaacata taaaaaacca agtcccattt gaaaccgtag ctaacgaata tggaatcaat
      601 ttcaaaaaca agaaagcaca ttgccccttt catgaagaaa aaacaccttc attccacaac
      661 tacggtactc atggaaaatg cttcggctgt gaaaaatctg ccgatgcaat cgagatagaa
      721 gcacacttca agggtttatc acccttacag gctgccaagt cacttgccga aagatataac
      781 ataaatattg aaacttatct ggataataat cacaattctg atttaagtga taccgatatt
      841 ccaaatgaga aatcacaaaa taaatcacaa tctgaaaagc ttgttgatct gattttaaat
      901 gatgacattg aactattcca tgattcagac aaaacagcat acgctcaaat aaaacgaggc
      961 agccactttg agtattacaa gatcagaggc aaagacttta gactttggtt gacgggtaac
     1021 tactgggata aatatcagaa aaccactaac tctaattcaa tggctgaagc attaaatata
     1081 gcagaagcca aggctctatt tgatggcaaa gagcataaac tatttaatag agttgcaaaa
     1141 agcgagataa caataatgta tgatcttgga aactggggtg gagtgcttat tcatgaaagc
     1201 ggttggtcga tacttgaatt actaccatcg gtatttaaaa gctaccctca ccaaaaaata
     1261 caggttgaac ctattaaatc ggagctaaat gaactttata gactgtttaa ttatatcaat
     1321 atccaggaag cagattatct ccttttcttg agtgacttaa cattcgcata tgttgcagac
     1381 ctcccgcacc ccatacttgc attaatgggt gattttggaa ctgccaagac tacagcggca
     1441 aaggttatta aatcactggt tgacccttcg gagcttgagg tactaacacc gccatctacg
     1501 aaacaggaac ttgcacagct tgcaagtcat cactgggtac taccgttcga taatttacac
     1561 acaataccgc agtggttttc tgattctcta tgtaagatag tctctggtga cggtttcagt
     1621 aaacgagaat tatacagcga tgacaacgat atattttact catataagcg taaatgcata
     1681 ctgacatcta ttaatcagat gctttccagc ccggatgtgc tatctcgctc tatcatcttc
     1741 cagcttgaac cgatacataa agataaaagg aaagaagaaa gtgtcttttg gaaggatttc
     1801 aacagagaaa agccaatgct ccttggtgcg atattctcac taatttctaa aacactggca
     1861 aacttagaca atgttaacca caagccattt tcaagactgg cagactttga aaagtggggc
     1921 tgtgctgtag cactgacact gggcagggaa gaagaagatt ttatcagtga atacaaaaac
     1981 aatatagagc tacaaaacag agaatctata gagtccagcc ccgtagcaaa gacactactt
     2041 tcttatatgg aatttaaaga atattgggaa ggcagtccaa gtgaattatt aaatgaacta
     2101 aatcctactg ctgaagaact aaaatattac agtgacagaa aatatcccaa agaccccaga
     2161 tggttatggc gtaggttgaa agaggtcagc acagatttat acacctatgg cattgaaata
     2221 aagcatgaag atacatgtca tggtgagcga gcaatatcca ttactaataa taat
//
LOCUS       DBNGSV010000312         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_610214, whole genome shotgun sequence.
ACCESSION   DBNGSV010000312 DBNGSV010000000
VERSION     DBNGSV010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_610214"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="AC159U_11235"
     CDS             <1..620
                     /locus_tag="AC159U_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337665.1"
                     /translation="ITGDITLLFYQQGSVSGYFSDFLDFIFHKYNLIEFSHSFWKFLG
                     TFTVFAEGFLSFGLWILRFRRFAIFIGMILHLGIGFTVSIETFSLQMIVLYIAFINPK
                     SHQNTVYYDSSNKRHRIFVRIGKMLDWLRRYNWVDNRPYKSPVINKKDISPSNIKSIS
                     LQTSDNEKRNGLSAVLYIFTLFPATFFFFYIPSALDNWRIKIKSI"
     gene            643..2019
                     /locus_tag="AC159U_11240"
     CDS             643..2019
                     /locus_tag="AC159U_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337666.1"
                     /translation="MNLYTKIRSLLMITVIFCICACSNQSQKENKEVNKKAVSVTKST
                     DDKPKNIYNFPDNLSKDEFPQWMKEFRKTRKWSKRNSKKLLNANILETSLQLGKQYLV
                     NNQTPEGNFVYLNYYMVNQPEEMDEADENEVRQAGALWGITTIYQYEQTPEVKTAIDR
                     GLKFFFKHTRKGPVEGSYIIDYPGMRYVKTGTVALVSLSIIDYLRTIKNEEITISQQY
                     QNELTDKLNGYLEFLNFMYLKDTKHFSQRYDPVKNEKWDNSSPYFDGETILCLIKAAK
                     YLGYTQYKPLIKESVLAMAKTYTTDQWHIDPQSRLTKGFFQWSLMAYWEYQDAGWENS
                     DLFRDYILSLSWWSIHINKIDEKKRNTSYAFEGLIFGYLVAKNNGNNAAASEILYIID
                     KVLYKLTTWQVGGPLEHENKFLIRTKPHKKSGIGGILNAKDFDNVRIDVVQHQMHAVL
                     LALRYIYN"
     gene            complement(2106..2720)
                     /locus_tag="AC159U_11245"
     CDS             complement(2106..2720)
                     /locus_tag="AC159U_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337667.1"
                     /translation="MDRQNILKGFLIIFSFVSFFMLFYISAFAKDDTIEGSIVCVNND
                     RFGKVNTITKYSSCSGVLVVLGNNSRIYALSGSDEQVREIEQSPQKIKKLKGQINGNE
                     RAWIFNISTLTPINEQTAKHEIMGDLYCLVPDDDNENIKAIISNESCDTKKPHAHVVS
                     TKDGEVYTIHGDQSAISALEKKSSRTELVLEGNLKKNGSELIID"
BASE COUNT          955 a          471 c          491 g          874 t
ORIGIN      
        1 ggattacagg ggatataacc ctgctgtttt atcagcaggg cagtgtaagc ggatattttt
       61 ctgattttct tgacttcata tttcacaaat acaatcttat cgaattcagc catagtttct
      121 ggaaattctt gggtacattt actgtatttg cggaaggttt cctgtctttt ggtctatgga
      181 tactgagatt cagacgattt gccattttta taggaatgat tctacatcta ggaattggtt
      241 ttacggttag cattgaaaca ttttcattgc agatgatagt gttgtatatt gcttttatca
      301 atccaaaatc tcaccagaac acagtatatt atgattcaag taataaacgg cataggatct
      361 ttgtaagaat cggtaaaatg ttggactggc ttagaagata taactgggtt gacaacagac
      421 cttataaatc gccagtaata aacaaaaaag atatatcacc atcaaatata aagagtatat
      481 ctttgcaaac ttcagacaac gaaaaaagaa acggtcttag tgctgtatta tatatattca
      541 ctctgtttcc agctacattt ttctttttct atattccttc ggctctggat aactggagaa
      601 taaaaataaa atctatttaa attaataagg agaatattta atatgaacct gtatacgaaa
      661 attagaagcc tgttgatgat tacagtcatt ttctgtatat gcgcctgtag taatcaatca
      721 caaaaggaaa acaaagaagt taataaaaaa gcggtatcgg tgactaaatc tacagatgat
      781 aaaccaaaaa acatttacaa tttccctgat aatctctcca aagacgaatt tcctcaatgg
      841 atgaaagaat ttagaaaaac tcgtaaatgg tctaaacgaa acagcaaaaa gttattaaat
      901 gcaaatatac ttgaaacctc gcttcagctc ggcaaacaat atctagttaa taatcaaaca
      961 cctgagggaa attttgtata tcttaattac tatatggtga atcaacccga agaaatggat
     1021 gaagcggacg agaatgaggt taggcaggct ggagcgctct ggggtattac gacaatatat
     1081 cagtatgagc aaactcctga agtcaaaaca gcaattgaca gaggactcaa atttttcttt
     1141 aaacatacca ggaaaggacc tgttgaaggc agttatataa ttgattatcc cggaatgcgg
     1201 tacgtaaaaa caggtactgt tgcactggtt tctctctcaa taatagatta tttaaggaca
     1261 ataaagaatg aagagataac aatatcccaa caatatcaaa atgaattaac agataaactg
     1321 aatggttatt tagaattttt gaatttcatg tatttaaaag atacaaaaca tttttcacaa
     1381 agatatgatc ctgtgaaaaa tgaaaaatgg gacaattcca gtccttactt tgatggcgaa
     1441 acaatacttt gtttgattaa agcggcaaaa tatctgggtt acactcaata taaaccgcta
     1501 attaaagaaa gtgttttggc aatggccaaa acttacacta cagaccaatg gcatatagat
     1561 ccgcaatcta gattaacaaa gggatttttt cagtggagcc tgatggcata ttgggaatac
     1621 caggatgccg ggtgggaaaa ttctgattta ttcagagact atattctaag cctttcatgg
     1681 tggtctatac atataaacaa aatagatgag aaaaaacgaa atacctctta tgcatttgaa
     1741 ggattaattt ttggatatct agtcgcaaaa aataacggta ataacgcggc agcttcggaa
     1801 atactctata taatagacaa ggtcttgtat aaacttacaa cctggcaggt tggcggcccg
     1861 ctggaacatg aaaataaatt tcttataaga accaagcctc acaaaaaatc cggaattgga
     1921 ggtatattaa atgcaaagga ttttgataat gtgaggatag atgtagtaca acatcagatg
     1981 catgcagtgc tgctcgctct tagatatatc tataactagt tgttatttat ccataattgt
     2041 ggatatcaat attaatacat ccgaattaaa aaaatatccc aataacaata gcctgatcta
     2101 attcactaat ctataatcag ttcagagccg ttttttttta agtttccttc aagaaccaac
     2161 tcagttcggg atgatttctt ttctaacgcc gatattgcac tttggtctcc atgaatagtg
     2221 taaacctctc cgtctttagt tgataccaca tgggcgtggg gctttttggt atcacaggac
     2281 tcatttgata ttatggcttt aatattttca ttgtcatcat ccggcacaag acagtacaaa
     2341 tctcccatta tttcgtgctt tgcagtctgc tcattaatag gagtaagagt agagatatta
     2401 aaaatccagg cccgttcatt gccgtttatt tgccccttaa gctttttgat tttttgtgga
     2461 ctctgttcta tttctcttac ctgttcatca gaacccgaaa gcgcgtaaat cctgctgtta
     2521 tttccaagca ccacaagaac cccgctgcag cttgaatatt tggtaattgt gtttactttg
     2581 ccaaatctgt cattattaac acagacaatg ctgccttcaa tcgtatcatc ttttgcaaat
     2641 gccgagatat aaaagagcat aaaaaaagat acgaaagaaa atatgataag aaatcctttt
     2701 aatatgtttt gtctgtccat cgaacttttt gcctgtgtta tttataaatt tagactcaga
     2761 caggccgtgt taagattctt aatatttata c
//
LOCUS       DBNGSV010000313         1432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1579767, whole genome shotgun sequence.
ACCESSION   DBNGSV010000313 DBNGSV010000000
VERSION     DBNGSV010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1579767"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..921
                     /locus_tag="AC159U_11250"
     CDS             184..921
                     /locus_tag="AC159U_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR3337668.1"
                     /translation="MFFFGAYFTFYKQLNDFLSKYQKNKTIFYEFNGKPSVKDSIEAI
                     GIPHTEVEHIMVNGVPVGFSYHIKNRDEIHVYPLYPDIKPSVPVKLRQEIEHAEFISD
                     VNLGKLARLLRMCGFDTLYNNSYDDHYIARLVAGSGRIVLTRDRRLLKYNDIVYGYWL
                     RSDDPHKQLKEVLDRYSIYESINEFKRCIRCNGFINKVDKETISDILEPLTKKYYNEF
                     YRCDSCRQIYWKGSHYEKMKNYLDSLV"
     gene            958..>1432
                     /locus_tag="AC159U_11255"
     CDS             958..>1432
                     /locus_tag="AC159U_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HIR3337669.1"
                     /translation="MKNNSNTKCPLGEHLQHYWDRLSEKEQEMQFDEEGLFSLALQKI
                     ALEIAQNTPGSTVVDAFCGAGGSAIGFALSGKNVIAIDSDAARLEMAKYNADIFGVSS
                     LIKFIEGDCLKILPTLNPDSIFLDPPWGGTDYGNIGKFKLLNFEPDGSKLLDLCFG"
BASE COUNT          449 a          216 c          332 g          435 t
ORIGIN      
        1 gcggttacag gtgattttgg agagggggtt gtagcgggcg ctgcaggcgg cggagcggca
       61 ggtttcatta gaggactatt cggttcacga gatttagaac cgttacaaag gcagtttgta
      121 aaccagtgtc ttgcggataa aggctataag attattggct gggactgaca cggaatgtag
      181 ataatgtttt ttttcggggc ctattttaca ttctacaagc agctaaatga ttttctttcc
      241 aaataccaaa aaaataaaac catattttac gagtttaatg gcaagccatc tgtaaaagac
      301 tcaattgaag ccattgggat tcctcatact gaggtagaac atatcatggt aaatggggta
      361 cctgttggat tttcctacca tataaaaaat agagacgaga ttcatgttta ccctttatat
      421 ccggatatta aaccttcagt tccggttaaa ttaaggcagg aaattgagca tgcagaattt
      481 atttcagatg ttaatctcgg caagcttgcg agacttttaa gaatgtgcgg gtttgatact
      541 ttatataata acagctacga cgatcactat atagcacggt tagtggcagg ttctggcagg
      601 atagtactta caagagatag aagactatta aagtacaatg atattgtata cggctactgg
      661 cttagatcgg acgatccaca taagcagtta aaagaagtcc tcgatcgcta tagtatttat
      721 gagagcataa atgaattcaa aaggtgtatt agatgtaatg ggtttattaa caaggtggat
      781 aaggagacga tatccgatat tttagagcct cttacaaaaa aatattataa tgaattttac
      841 aggtgcgatt catgcagaca gatttactgg aaggggtcgc attacgaaaa aatgaaaaac
      901 tatcttgatt cactggtata agattcaatt tatgaatcca atctttttag tatgacaatg
      961 aaaaataatt caaatactaa atgtccgttg ggagaacatt tacagcacta ctgggacaga
     1021 ttgtctgaaa aagagcaaga aatgcagttt gatgaggagg ggttgttttc attggctctt
     1081 cagaaaattg ctcttgagat tgctcaaaat actccgggaa gtacagtagt agatgcgttt
     1141 tgcggagcgg gtggaagcgc aattggcttt gcattaagcg gcaagaatgt gattgctatt
     1201 gatagtgatg ctgcaaggtt ggagatggca aaatataatg cagatatttt tggggtttct
     1261 tctcttatca agtttattga aggtgactgc ttgaaaattc taccgacctt aaaccctgat
     1321 tcaattttct tggatcctcc ctggggtggt acggattatg ggaatatcgg caagtttaaa
     1381 ctattaaatt tcgagcctga cggaagtaaa ttacttgatt tatgttttgg ca
//
LOCUS       DBNGSV010000314         3137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1648721, whole genome shotgun sequence.
ACCESSION   DBNGSV010000314 DBNGSV010000000
VERSION     DBNGSV010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1648721"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="AC159U_11260"
     CDS             <1..385
                     /locus_tag="AC159U_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3337670.1"
                     /translation="HEWGQFMYRATGDYALFYWIMVVCNVVVPISLWFKKIRHNIKAL
                     FIISLLINIGMWFERFNIIVISLSHEFDPGNWGFYKPSLIELGITAGSFAWFFMFFLL
                     FIKTLPAVAIAEMKEILPVPKKGAK"
     gene            388..909
                     /locus_tag="AC159U_11265"
     CDS             388..909
                     /locus_tag="AC159U_11265"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR3337671.1"
                     /translation="MKKNQGVVGVYSYVDLVLEAIRRLKNAGHEKKDIRVFSPVPNHE
                     IEDALIESESIVRFFTLTGATLGAICGIGITTLTSMDWPIQVSAKPIVSMPPYMIIVF
                     ELTVLMGALSTFIGLIVNARIRRNAPVSLYDPRFSDDKFGLAVVCEKDRVGDVEKIMN
                     DTEAEEVKFEGLH"
     gene            940..1446
                     /locus_tag="AC159U_11270"
     CDS             940..1446
                     /locus_tag="AC159U_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3337672.1"
                     /translation="MFGRTKLLILTTLIIFISASISVIGLPWNYDMWKQKSIKPYEMP
                     MSYPKGTVTIDGIVNPEKREKVEAITQNGLEASKLDVKIGEQLYMTNCSVCHGEKADG
                     NGPVIKKGFYPVNLKVQAVKDRTDGYIFAYIRYGGKVMMPSYRENMTEEQSWQVVNYI
                     RHIQNSSK"
     gene            1472..2728
                     /locus_tag="AC159U_11275"
     CDS             1472..2728
                     /locus_tag="AC159U_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337673.1"
                     /translation="MDESIRDEILAKRPQISPLLWVVFGSLFIIGVFVFLTNAFSEEH
                     SHDTWQIFLVNFVFWTGIAQAGIVFSAILRLTRGKWGRPLLRVSEALGSFVPVSFILL
                     LVLLFIGKDYILPYIIEPYPYAAKEVWLAPGFVVGRHLIAFGLLFILSMIYLYYSLRQ
                     DLGGAGEKLTGICANIAEGWKGDDEREEIWAKIVKLCPAIAIVYAFTFSLIGWDFMMS
                     LDPHWFSTLFGPYYFMGSILGAVGMIIIMSSYLRKHMQLGEYITDHQYFDIGKIMLGL
                     SMFWVYLTFSQFLPIWYANMPEETGFVIARTQVEPFATLCWVILTCCFFFPFVSLIPR
                     TTKVVNQLTIFIAVVSLSGLWLEKYVLIVPSISHGIHFGFIEILITAGFASGFVLTFL
                     CFIKIFPILPVGDPMFVGKAHGHGGH"
     gene            2733..2873
                     /locus_tag="AC159U_11280"
     CDS             2733..2873
                     /locus_tag="AC159U_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337674.1"
                     /translation="MDRLLDFITQIVCGALIFGVFGFIATLVAISLFGPQLPELFQGL
                     PW"
BASE COUNT          960 a          497 c          645 g         1035 t
ORIGIN      
        1 gcatgaatgg ggccagttta tgtacagggc aacaggagac tacgcactat tttactggat
       61 catggtcgtt tgcaacgtag ttgttccaat ttcgctttgg tttaagaaaa taagacataa
      121 tattaaagcg ctttttatta tttcactttt aattaacatt ggaatgtggt ttgaaaggtt
      181 taatatcatc gtaatatcac tctctcacga gtttgatccg ggtaactggg gattttataa
      241 accatctttg atagaacttg ggatcacagc gggtagcttt gcatggttct ttatgttctt
      301 cttgctgttt ataaaaactc ttccggcagt tgcgatagct gagatgaaag aaatactacc
      361 ggttcctaaa aagggggcta aatagatatg aaaaagaatc agggtgtagt tggagtatat
      421 tcatacgtag atttagtctt agaagccata agaagactta aaaacgcggg acatgagaaa
      481 aaagatatca gagttttttc tcctgtacca aaccatgaaa ttgaagatgc actaattgaa
      541 agtgagagta tcgtaagatt ttttaccctc actggcgcta cccttggagc tatatgcgga
      601 attggaataa ctactcttac atcaatggac tggccgattc aggttagtgc caaacccatt
      661 gtatctatgc ctccctatat gattatcgta tttgagctta ctgttcttat gggagcatta
      721 tcaacattta taggtttaat tgtaaatgcg aggattagaa gaaacgcacc tgtaagtctg
      781 tacgatccaa ggttttctga tgataaattt gggctcgctg tagtatgtga aaaagacaga
      841 gtgggtgatg tggaaaagat aatgaatgat acagaggctg aagaagtcaa atttgagggg
      901 ctgcactagc cttataaatt tatattgagg agaaaggttt tgtttggaag aacaaaatta
      961 ttaatattaa caacacttat aatattcata tcagcttcaa tttcagtaat tgggcttccg
     1021 tggaactatg atatgtggaa gcaaaaatcg attaagcctt atgaaatgcc gatgtcttat
     1081 cccaagggta cagtaacaat agacggaatt gtaaatcctg aaaagagaga gaaggtagag
     1141 gctataaccc aaaacggact ggaagcttcg aagctcgatg taaaaatagg tgaacagctt
     1201 tatatgacaa attgttcagt ttgtcatggt gaaaaagccg atggaaacgg tcccgtcatt
     1261 aaaaagggtt tttatccggt taatttaaaa gtgcaggcag taaaagatag aactgatgga
     1321 tatatatttg catacataag atatggcgga aaagtaatga tgccaagtta ccgtgagaat
     1381 atgactgaag aacagtcatg gcaggtagta aattatataa gacatataca gaatagttct
     1441 aaatagttta ggagagtatc aggaatacaa catggatgag tcaataagag acgaaatatt
     1501 agctaaaaga cctcaaattt caccacttct ttgggtggtt tttggctctt tatttataat
     1561 cggtgtattt gtatttttaa caaacgcctt ctcagaggaa cattcacacg acacctggca
     1621 gatcttttta gttaattttg tgttctggac cggtattgca caggcaggaa ttgtgttttc
     1681 agcaatttta agacttacaa gaggaaaatg gggcagacca cttctaagag taagtgaggc
     1741 gctgggttca tttgtgccag tatcctttat acttcttctg gtattactat ttataggtaa
     1801 agattatatt cttccttata ttatagagcc ctatccttat gctgcaaaag aagtatggct
     1861 tgcgccgggt tttgtagttg gaagacacct gatcgctttt ggactcttgt ttatacttag
     1921 tatgatttat ctctactatt cactaaggca ggatcttggg ggtgcaggag aaaaattaac
     1981 cggtatatgt gcaaatattg ccgagggctg gaaaggagat gatgaaagag aagagatatg
     2041 ggcgaaaata gtaaaacttt gccctgcaat agcaatagta tatgcattta cttttagcct
     2101 tatcggctgg gattttatga tgtcgctcga tcctcactgg ttcagcacgc tttttggccc
     2161 ttattatttc atgggaagta ttttaggcgc agttggaatg attataatca tgtcttctta
     2221 cttaagaaag cacatgcagc ttggagaata tatcacggac catcagtact ttgatattgg
     2281 caagattatg cttggtctgt cgatgttttg ggtatacctt acattttctc agtttttacc
     2341 aatttggtat gcaaatatgc ctgaggaaac aggatttgtt atagccagga cgcaggtaga
     2401 gccatttgca acactttgtt gggttattct tacatgctgt ttcttcttcc catttgtatc
     2461 ccttatacca agaactacaa aagtagtaaa tcagttaaca atattcattg cggtagtttc
     2521 gctttcagga ctatggcttg aaaaatatgt gcttatcgta ccatcaatat ctcacggtat
     2581 tcactttgga tttattgaaa tattaataac agccggtttt gcgtccgggt ttgttttaac
     2641 atttctatgt tttatcaaaa tctttccaat actgcctgta ggcgatccta tgtttgtagg
     2701 taaagcacac ggacacggag gccactaata atatggacag actacttgac tttattactc
     2761 agatagtgtg tggagctctt atattcggtg tatttggctt tattgcaaca ctggttgcta
     2821 tctctttatt tggtccacaa cttccagaat tgtttcaggg ccttccctgg taaaataaat
     2881 tatttatagc tatttttata aatttagttg taaattattc aacatagcat taaaattcta
     2941 taaataatta gggccactta ttttaaattc taattatttt tgttctaata gcttgtttct
     3001 agcagtattt atttaggagc tcgcatagaa cagagctata agtaatgcag aatctccttt
     3061 attttattaa ctaaatctac atttctgtta ttaaatctaa attccatctc ttttagatac
     3121 aaaacaaagt gttttct
//
LOCUS       DBNGSV010000315         5612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1648848, whole genome shotgun sequence.
ACCESSION   DBNGSV010000315 DBNGSV010000000
VERSION     DBNGSV010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1648848"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..409
                     /locus_tag="AC159U_11285"
     CDS             191..409
                     /locus_tag="AC159U_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337675.1"
                     /translation="MLKLLMKHRISSKHIPQVLVKMRVGGSSNKSLLNILKANIECYR
                     AWKEHDLEISPFFILRKPFNKLTQYYVN"
     gene            477..1517
                     /gene="rfbB"
                     /locus_tag="AC159U_11290"
     CDS             477..1517
                     /gene="rfbB"
                     /locus_tag="AC159U_11290"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3337676.1"
                     /translation="MTKHTVLLTGGAGFIGSNFIINVLKRFNSLNIINLDKLTYAGNL
                     NNLKEVSKNKRYRFEKGDICNRLFVDKLFKKYAFDVVVNFAAESHVDRSILKPGSFVK
                     TNIVGPQILLDAIKANPVKKFIQISTDEVYGEKRKGKSKEFDILNPNNPYSASKACAD
                     LLCRSYTKTFGLPVIIVRLSNNYGPYQFPEKLVPLMILNAINYKHLPVYGSGKNIRDW
                     MYVEDSCNAIIKIIKQGEPGEVYNIGASNLQSNIKFVQKICRLVSKKIKISSEEILSR
                     IEYIADRPAHDARYCLDTSKIYNETGWTPKTDLDRGLEKTVNWYLNNKTWVTNVMNKD
                     YLKYYKLNYTNR"
     gene            1532..2218
                     /locus_tag="AC159U_11295"
     CDS             1532..2218
                     /locus_tag="AC159U_11295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3337677.1"
                     /translation="MGNITLNTMDNNNIYISDKDEPREYSLKRIIDFLLALGGLVASF
                     PLTIIVSILVYLEDRGPVFYSQKRVGKGGILFNTYKFRTMIEDSDRLFGPLQAKENDP
                     RITKVGKLLRFTALDELPQLWSILKGDMSFVGPRALLPAEKETGDDKNNEMVPANNIK
                     GYEKRILVKPGLTGIAQIFAPRDINRRNKFRYDSIYIDNMGLLFDLKLICISFYITFK
                     GAWEKRESKI"
     gene            2308..3249
                     /locus_tag="AC159U_11300"
     CDS             2308..3249
                     /locus_tag="AC159U_11300"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR3337678.1"
                     /translation="MKKRILVTGGAGFIGSHLCEKLLSEGNEVVCVDNFFTGTKDNIK
                     RFLSNPYFEYIRHDITFPLFIEVDEIYNLACPASPIHYQFDPVQTTKVNVLGSINMLG
                     LAKRLKVKILQASTSEIYGNPLEHPQTESYWGNVNSIGPRACYDEGKRCAETLFFDYY
                     RQHSLDIKVVRIFNTYGPRMHPNDGRVVSNFIIQALKGEDITVYGEGSQTRSFCYVDD
                     LVDGIYKMMNINSGFTGPVNLGNPVETTVLELANFIIKLTGSKSKVIFHDLPQDDPEK
                     RKPDISLAKDKLDWEPGVKLEDGLIKTIEYFNKMKQL"
     gene            3246..4289
                     /gene="gmd"
                     /locus_tag="AC159U_11305"
     CDS             3246..4289
                     /gene="gmd"
                     /locus_tag="AC159U_11305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3337679.1"
                     /translation="MKKAFITGITGQDGSYLAELLLSKGYEVHGLIRRASTYNTARID
                     HIYVDPHNPNAKLYLHYGDLSETSQLTNLLYSLKPDEIYHLGAQSHVRVSFDIPEYTE
                     DITGIGTVRILEAIRRGDLNSRFYQASSSEMYGSSSAPQSETTPFHPRSPYACAKLMS
                     FWTTANYREGYNLFACNGILFNHESPRRGQNFLTRKVTRAIAMIKDGKQDCLYLGNLD
                     ACRDWGYAPEYVELMWKILQHEKPDDYVIGTGETHSVQEFVEEAFSYVGLDFEKYVKI
                     DPKYYRPTEVENLQADPTKAKTELDWELKIKFKDLIKIMIDSDMRAEGLDPVGEGDKL
                     LDKYYPNRWWKVD"
     gene            4298..5245
                     /locus_tag="AC159U_11310"
     CDS             4298..5245
                     /locus_tag="AC159U_11310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR3337680.1"
                     /translation="MQDYFKSKQILITGGAGFLGSFIIDQLVEKGVERKNIRVPRSKD
                     CDLRIWENCKKAIEDIDIVIHLAARVGGIGFNMKYPANLFYDNAMMGIQLLEASRLSG
                     VKKFVGVGTVCSYPKYTPVPFKEEDLWNGFPEETNSPYGIAKKILLVQEQAYRTQYGF
                     NSIYLIPVNLYGPRDNFDTEDSHVIPALILKFTEAIRKNAPSVTVWGTGKVSREFLYV
                     EDAARAILEATEKYNKPDPVNIGTSEEIMIEDLVYLVKDLTGYSGDVVWDLEKPDGQP
                     KRKLDVSKAEREFGFKAEVNLQQGLKKTIEWFNKQYNES"
     gene            complement(5267..>5612)
                     /locus_tag="AC159U_11315"
     CDS             complement(5267..>5612)
                     /locus_tag="AC159U_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3337681.1"
                     /translation="SPGIGWANTERRSIKDRGPADVIFALALVHHLAISNNVPLEKIA
                     QYLSTLCSYLIIEFIPKEDSKVQYLLSSREDIFTDYHKSGFEAAFSKYFSIIKQSPVL
                     ESHRTLYLMNKK"
BASE COUNT         1981 a          824 c         1124 g         1683 t
ORIGIN      
        1 aagcgaatgc tatctgttgt tctgtaaatc ttgacttcct catggcaaaa tacctcctga
       61 tttattgcat tattttgcca gatttctcta cttttacctg gttcagtttg cagggtgaag
      121 gtcaaaaaat atttatgaaa aatatggagt attcaataca aagtataaaa taggcgccga
      181 ttacgcattg atgttgaaat tattaatgaa acacaggatc agttcaaagc atattccaca
      241 agtgctggtt aaaatgaggg ttggaggcag cagtaacaag agtttattaa atatacttaa
      301 agcaaatatc gaatgttata gggcctggaa ggagcatgat ttagaaattt caccgttttt
      361 tatattaaga aaaccattta acaagcttac tcagtattat gttaattaaa ttgtttattg
      421 gtttaataga atttatttat agttcacgcc taagcaaata gtatacattt tattatatga
      481 ctaagcatac agtattattg acaggcggtg ctgggtttat aggcagcaat tttataataa
      541 atgtattaaa gaggtttaat tctctcaaca ttataaacct tgataagctt acatatgcag
      601 gaaatttaaa taaccttaaa gaagtaagta aaaataaaag gtatagattc gaaaagggag
      661 atatttgtaa cagactattt gttgataaat tatttaaaaa atacgccttt gatgttgttg
      721 taaactttgc agcggaatct catgttgata gaagcatcct taaacctggc agttttgtaa
      781 aaaccaatat agtaggaccg caaatactac ttgatgccat taaagcaaat ccagtcaaaa
      841 agtttataca aatatctaca gatgaggtat atggtgaaaa aaggaaaggt aaatctaaag
      901 agtttgatat attaaatccg aacaatccat attctgccag caaggcttgt gctgatctcc
      961 tttgtagatc atatactaaa accttcgggt tgccggtaat aattgtgcgt ctttcaaata
     1021 attatggtcc ctatcaattt ccggaaaaat tagtgccgct tatgatatta aatgcaataa
     1081 attataaaca tcttccggta tacggaagcg gcaaaaatat cagagactgg atgtatgtag
     1141 aagatagctg taatgcaatt ataaagatta taaaacaagg agagcctggg gaggtctata
     1201 atataggggc tagtaattta caaagtaata ttaagtttgt tcaaaaaata tgcaggttag
     1261 tatctaaaaa aatcaaaatt agtagtgaag agattttgtc tcgtatagaa tatatagcag
     1321 acagacctgc gcatgatgca agatattgcc ttgatacttc aaaaatatat aatgagaccg
     1381 gatggacccc taaaacagat ctggatcgtg gtcttgaaaa aactgttaac tggtatttga
     1441 ataataaaac ctgggttact aatgtaatga ataaggatta tcttaaatat tataaattga
     1501 attatactaa tagataataa gttttataat tatgggaaat attacactta atactatgga
     1561 taacaataat atctatatat cggataaaga tgaacccaga gagtattctt tgaaaagaat
     1621 catagatttc ttattagcac tcggtgggtt agtggcgtca tttcctttaa ctattatagt
     1681 ctctatatta gtttatctcg aagatagagg gcctgtattt tattctcaaa aaagagttgg
     1741 aaaaggtgga atattgttta atacatataa atttagaaca atgatagaag actcagacag
     1801 acttttcggg ccattacagg caaaagaaaa tgatcccagg ataactaagg tcgggaagtt
     1861 gcttcgtttt acagcactag atgaattgcc tcaactatgg tcaatactta aaggtgatat
     1921 gagttttgtg ggacccaggg cgcttttgcc ggccgagaaa gaaaccggtg atgataagaa
     1981 taatgaaatg gttccggcga acaatataaa aggttatgaa aaaagaatac ttgttaaacc
     2041 gggtcttacg ggaatagccc agatatttgc accaagagat attaatagaa gaaataaatt
     2101 cagatatgac agtatatata ttgacaatat gggattacta tttgatctta aacttatttg
     2161 tatttcattt tatataacat tcaaaggagc atgggaaaaa agggaaagta aaatatagat
     2221 gtcataaaag cgtaacaatt ttgaattatt caatttctca ggtttatttc aactcagtta
     2281 aataattaaa ttcttcggat atcaacaatg aaaaaaagaa tattagttac cggaggagcc
     2341 ggatttattg gttctcactt atgtgagaag ctactcagtg aaggtaatga ggtagtatgc
     2401 gtggataact tttttactgg tacaaaagat aacattaaga gatttttatc caatccatac
     2461 tttgaatata taaggcacga tattacattt ccgctcttta tagaagtaga tgagatttat
     2521 aaccttgctt gtcctgcttc accaattcat tatcagtttg atccagttca gactactaag
     2581 gttaatgtgc ttgggtctat aaatatgctc ggtcttgcaa aacgattgaa agttaaaatt
     2641 ttacaggcat cgacaagtga aatatatgga aatcctttag agcatcccca gactgaaagt
     2701 tattggggaa atgttaatag cattggacct agagcatgtt acgatgaagg taaaagatgt
     2761 gcagaaacct tattttttga ttactacaga cagcacagct tggatattaa agtagtaaga
     2821 atattcaaca catatggtcc caggatgcac ccaaatgacg gccgagttgt aagcaacttt
     2881 ataatacagg ccttaaaagg agaagatata acagtatatg gtgaaggatc acagacaagg
     2941 tcattttgtt atgttgatga cttagttgat ggaatatata agatgatgaa tataaacagt
     3001 ggatttactg gccctgttaa tctggggaat cctgtggaga ctaccgtgct tgaattagca
     3061 aacttcataa ttaaactaac agggtcaaaa tctaaagtaa tttttcatga ccttcctcaa
     3121 gatgaccctg aaaaaagaaa acctgatata tcactagcta aagataaact ggattgggaa
     3181 cccggagtaa agctggaaga cggactgata aaaactatag aatattttaa taagatgaaa
     3241 caattatgaa aaaagcattt attaccggaa ttaccggaca agatgggtca tacttagcgg
     3301 agcttctact ttcaaaagga tatgaagttc atggactaat aaggagagcc agtacttata
     3361 atactgcaag aatagaccat atttatgttg atcctcataa cccaaatgcc aaactttatc
     3421 tccattatgg tgatctttct gagacttctc agttaacaaa cctcttatac agcctgaagc
     3481 cggatgagat atatcacctg ggagcccaaa gtcatgtaag ggtgagtttt gatattcctg
     3541 aatataccga ggacattacc ggtattggca cagtaaggat actggaggca ataagaagag
     3601 gggatctaaa tagtagattc taccaggcat caagcagtga aatgtatgga agttcttctg
     3661 cacctcaaag tgaaaccact ccatttcatc ctagaagccc atatgcttgt gcaaagctta
     3721 tgtctttttg gaccactgca aactatagag agggatacaa tctgtttgca tgcaatggaa
     3781 ttttatttaa tcatgagtct ccaaggagag ggcagaactt tctcactaga aaggttacaa
     3841 gggctatagc aatgattaaa gacggtaagc aggactgtct ctatcttggt aatttggatg
     3901 catgcagaga ctggggttat gctccagagt atgttgagct tatgtggaaa atactccagc
     3961 atgaaaagcc agatgattat gttataggaa ctggggagac tcatagtgtg caagagtttg
     4021 ttgaagaggc tttttcgtat gttggtcttg attttgagaa gtatgtaaaa atcgacccta
     4081 aatattatag acctactgaa gtggaaaatc tccaggccga tccaactaaa gcaaaaacag
     4141 aattagactg ggaattgaaa attaagttta aagatttaat aaaaatcatg attgactctg
     4201 atatgagagc agagggtctc gaccctgttg gtgagggtga taaattgtta gataagtatt
     4261 atcccaacag atggtggaaa gttgattaag agacaaaatg caggattatt ttaaaagtaa
     4321 acaaatctta atcacaggcg gagcaggttt tcttggctct tttattatag atcaattagt
     4381 agaaaaaggc gtagaaagaa aaaacattag agtaccaaga agtaaggatt gtgacttaag
     4441 aatatgggaa aattgtaaaa aagcaataga ggatatagac attgttattc atctggcagc
     4501 cagggtgggt ggtatcggtt ttaatatgaa ataccctgct aatttatttt atgataatgc
     4561 tatgatgggc atacagcttc tagaagcatc aagactttca ggggttaaaa aatttgttgg
     4621 tgtaggtaca gtgtgcagct atcccaaata tacacccgta ccatttaaag aggaagattt
     4681 atggaacgga tttcctgaag agacgaactc tccttatggt atagctaaaa agatattact
     4741 tgtgcaagag caggcatata gaacacagta cggttttaac tctatttatt taataccggt
     4801 aaatctttat ggtccaaggg acaatttcga taccgaagat tctcatgtga ttccagcact
     4861 tatactgaaa tttactgaag ctatcagaaa aaatgctcct tctgtcacag tatggggaac
     4921 tggtaaagta tctagagagt ttttatatgt agaagatgca gcaagggcaa tactggaagc
     4981 tacagaaaaa tataataaac cagacccagt aaatattggt acatcagaag aaataatgat
     5041 tgaagacctg gtttatttag taaaggattt aaccggttac tctggtgatg tagtatggga
     5101 tttagaaaaa ccggacggac agccaaagag gaagcttgat gtttcaaagg ctgaaagaga
     5161 atttggtttc aaagcagagg taaatctgca gcagggtctt aaaaaaacta tcgagtggtt
     5221 taataagcag tacaatgaaa gctaggtcag ttgtatcagt aagagattac ttcttgttca
     5281 tcagatataa agttctatgt gattctaata ccgggctttg ctttataata gagaaatatt
     5341 tgctaaatgc cgcctcaaag ccggacttat gataatctgt aaatatatct tctcttgacg
     5401 acaataaata ctgtaccttt gaatcttctt ttggaataaa ttctatgatt aaataactgc
     5461 acagtgtact taaatattgt gcaattttct caagaggcac attgttagaa attgccagat
     5521 gatgcacaag agcaagcgca aaaattacat cggctgggcc tctgtcctta attgaccttc
     5581 tttcagtatt tgcccatcct atgccagggc tt
//
LOCUS       DBNGSV010000316         9884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1303576, whole genome shotgun sequence.
ACCESSION   DBNGSV010000316 DBNGSV010000000
VERSION     DBNGSV010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1303576"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..654)
                     /locus_tag="AC159U_11320"
     CDS             complement(76..654)
                     /locus_tag="AC159U_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337682.1"
                     /translation="MKLRMPFNKAEENSGINQGENNMADDSSKVALQQDTALEEQLML
                     LLSNNQVITVKLTEDKIIKDPKEGLRLLCDLVNQVVGFAEKKLRVNSSYLQKLLVSQS
                     ENYPNIKLLYLKENRLSPDTVINLFKGWASHPSDRQPIFDEIREALLNIIQSYFSLFE
                     SSFRSDLIKKQWQEIYLIHIDELREMASKIKF"
     gene            complement(655..2751)
                     /locus_tag="AC159U_11325"
     CDS             complement(655..2751)
                     /locus_tag="AC159U_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3337683.1"
                     /translation="MNVKLAKLFIEKGYLSLDNLKEAVEEQKNNNKSLGEILIRKGYI
                     TESQLVELTNIDNQFPRVDLSAINIEPSVIKLIPQDTAVDNNLIPFDLEGAFLSVAMI
                     DPTNIVLIDELRFLTGHNIKPYIAEFSSIKSALVNHHNIDAEQVVQKQEPEPLQKAEQ
                     EQTIVQKPEEQQIVQPSEPEPEVAAQAPSEPAQPEPEPQIETQPQSYPEPPVSQSQQT
                     DHSVFTASPQVSSEKTETADTLASSLTTEPESQAQPPQIDEPQFPGQSPQPQPQATDE
                     KPNQNEIIDVFAKDDDDYVRGVKQSSDSSFENISLQSSDSSSNLFQPPAESREVDARP
                     HSEQTESEPASTAGGEQSLFQAPAEQAGATVGFQPVESQKSEPINIPVPPQAQDSSPE
                     LNVFSSSQSASEEDDDKTIELSLEAEEITDQIEVPDNKDVFTIPTMQTESAGTETDLA
                     PDAADSQPGATAPTQSADPGKEVEDLFQIPGQKESEIGNVVPPVNNETDLRSLNEDSS
                     YDNIESFNKTIDSNGNHVGTTVSAEPPKPELREVEPDIPPPPPPVNEPVIPVQDQYAQ
                     PADTVSKPTILVVDDSPTVQKIVSVTLSRHGYQVEVSSNAMQALAKLNEFIPDIIFLD
                     INLPHMDGYQLCKIIKGNELTKEVPVIMLSGKDGIIDKMRGKLVGATDYITKPFEPNS
                     LVSAIKKQGVSVAGTA"
     gene            3211..3549
                     /locus_tag="AC159U_11330"
     CDS             3211..3549
                     /locus_tag="AC159U_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3337684.1"
                     /translation="MKKIEAIIKPFKLDDVKEALKDIGIQGLTVTEVKGFGRQKGHTE
                     LYRGAEYVIDFLPKIKLEIIVSDDTVTKVVDAIVESARTGKIGDGKIFILPMEEVIRI
                     RTGERGEDAL"
     gene            3604..5055
                     /gene="glnA"
                     /locus_tag="AC159U_11335"
     CDS             3604..5055
                     /gene="glnA"
                     /locus_tag="AC159U_11335"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3337685.1"
                     /translation="MAKSTKGGAQSPPNNANDVLKLVKDENIQIIDFRFLDFVGMWQH
                     FSTPTHELDKEAFEEGLGFDGSSIRGWQSIHASDMLMIPDPKTARIDPFTNIPTLALI
                     CDIVDPITREPYSRDPRNIAKKAVSYLQSTGIADTAYFGPELEFFILDDVRYDQTANT
                     GYYFVDSVEGIWNSGTDEGPNLGYKPRHKEGYFPCPPTDSYQDLRSEMVLEMENLGIA
                     VEAHHHEVSTAGQAEIDMRFSPLVDMGDNMMWYKYICRNVARKYGKTVTFMPKVIFED
                     NGSGMHIHQSLWKKGKPLFAGNSYAGMSEMAMHYIGGILKHAKAICAFSNPTTNSYRR
                     LVPGFEAPVNLAYSARNRSAAVRIPMYSPSPKAKRLETRFPDPSANGYLTFSAMLMAG
                     LDGIENKIDPGDPLDKDIYSLGPEELEGIPYVPGSLEEALNSLKEDHEFLTKGDTFTD
                     DVVETWIDYKMENEVNPVKLRPVPHEFMLYFDV"
     gene            complement(5272..7233)
                     /gene="nuoL"
                     /locus_tag="AC159U_11340"
     CDS             complement(5272..7233)
                     /gene="nuoL"
                     /locus_tag="AC159U_11340"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3337686.1"
                     /translation="MLFLIILLPLIGAAINGLLFTTGLIKQDHKSEKSIVSVIACSSI
                     LFSALIATYMFYTLVGSEGLTGIVETSFSWIVSGELDIKFGFLFDNISAIMTLVITWV
                     GFLIHVYSIGYMSHDKSYARYFTYLNLFVFFMLVLVLGNNFPLMFVGWEGVGLASYLL
                     IGFWYDDDQKALAGRKAFIVNRIGDFGFLIAIFLIFITFGSLSYADVFSQASSQAILN
                     NIPAITITAIALLLFVGAVGKSAQFPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMV
                     TRCAVFFSASPVAQFIVVTIAVFTAFFAATIALSQTDIKKVLAYSTVSQLGFMFMGVG
                     IGAYAAGMFHLVTHAFFKALLFLGAGSVIHAMSDEQDVRKMGGLRKYLPITSKTFLVG
                     TLAISGLPLLSGFFSKDQILGELYEKGFAIHWAVALVTAMLTALYMMRLYFLTFEGKS
                     RVPANIMKKLHESPSTMTIPLIVLAVLSVIGGYVGVPDFIGEMFGLHHVNKFKEFIWA
                     AIPLEQHHASAVSHHFFSHMDLLILSIVAAVLGIGLAYYFYIYRPKHLSKITANEDFG
                     FLFNASNSKWYMDEVYEHTFIDGFKESSKMSFWFDSNIVDGLVNRIAGFFSCLSTFVR
                     KFQFGLVRAYAAVMTAGLILILYIIFSTF"
     gene            complement(7244..7549)
                     /gene="nuoK"
                     /locus_tag="AC159U_11345"
     CDS             complement(7244..7549)
                     /gene="nuoK"
                     /locus_tag="AC159U_11345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3337687.1"
                     /translation="MEVTINHYVLLSFMLFVIGAFGVITRKNLIIVLMSLELMLNAAN
                     LALVSFSRFHGDNTGQVFMLMSLVVAACEVAVGLALVVSIYHNKETINIDVLKLLKG"
     gene            complement(7550..8062)
                     /locus_tag="AC159U_11350"
     CDS             complement(7550..8062)
                     /locus_tag="AC159U_11350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR3337688.1"
                     /translation="MDTYLFYFFACAVLASSIAVVVNRNPVASIVALVLTLFNTSVLY
                     IMLSAQFVAVVQILVYAGAIMVLFMFTVMFLILRDESLNFDAQNVPLKVSSALAVLFI
                     TAMIVQNVVIGTSKPAKLSDISVSEDYGSVENVGTSIFTYYILPFELTSVLILIAIIG
                     VIVLSKRSAD"
     gene            complement(8174..8554)
                     /locus_tag="AC159U_11355"
     CDS             complement(8174..8554)
                     /locus_tag="AC159U_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337689.1"
                     /translation="MGAYAMAAYGYTRSTGDFDIWVENSSENSVKLYKVLSDFGAPLK
                     DITPETFSNKDIVYQIGVAPVRIDVLTDIDGVSFKDAYKSKHDLVLDGLLLPFISKSD
                     LIKNKRSTGRDRDVLDAQTLEDLE"
     gene            complement(8565..8759)
                     /locus_tag="AC159U_11360"
     CDS             complement(8565..8759)
                     /locus_tag="AC159U_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337690.1"
                     /translation="MRLKKNIEAIKRLKDNDSGFVDASPQERISMVWELTREVWYFKD
                     RKNVEQRLQRDVEKLIRKSG"
     gene            complement(8778..9434)
                     /locus_tag="AC159U_11365"
     CDS             complement(8778..9434)
                     /locus_tag="AC159U_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3337691.1"
                     /translation="MEDININEVVKVLKKEYKRFKTPFVSVEADKSKKAFNVLISTII
                     SLRTKDKTTEEASVRLLKLASNSKDMLKLKIPQIEKAIYPAGFYRVKAKTIKEVCKEL
                     IDKYESRVPDDLDELLKLKGVGRKTANLVITLGYNKPGICVDTHVHRIFNRLGYIKTK
                     TPDESEFALREKLPKRYWIDINSLLVAYGQNLCKPIGPKCDICKMEDMCRKVGVKKLF
                     "
     gene            9496..>9884
                     /locus_tag="AC159U_11370"
     CDS             9496..>9884
                     /locus_tag="AC159U_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337692.1"
                     /translation="MEYPKLRFVDTFPIEHEGGKYVCLRDPQDTNGNMLLVAPQAIYV
                     ISYFTGSNSIVDIQLKILKEYGELIEKDHIIQLAKQLDDALLLDSDKYRDHQLQLEND
                     FKKSKIREASHAGMSYPNEKEELTKWI"
BASE COUNT         3134 a         1924 c         1769 g         3057 t
ORIGIN      
        1 tattattgga atctttatta tcagacataa tattagttct ccgtctttat attattatat
       61 aatttaatga tttgtttaaa atttaatctt agaagccatc tctcttaatt catctatatg
      121 aattaaatat atttcctgcc actgcttctt tattaaatct gatctaaagc tcgattcaaa
      181 caaactgaaa taggactgaa tgatatttag aagagcttct cgaatttcat caaaaatcgg
      241 ctgccggtct gatggatgac tagcccagcc cttaaataaa tttatgacag tatcaggtga
      301 taatctattt tctttcagat acagcagctt tatatttgga tagttttcac tctgcgatac
      361 taaaagcttt tgcagataag atgaattaac ccttaacttt ttttcggcaa accctacaac
      421 ctgatttacc agatcacaca gtagtcttaa tccttcctta ggatctttta ttatcttgtc
      481 ttcggtaagc tttacagtaa taacctgatt atttgataga agcagcatta gttgttcttc
      541 caaagctgta tcctgctgta acgctacttt tgaactatca tcagccatgt tattttcacc
      601 ctgattaata ccgctatttt cttctgcttt attaaatggc attctaagct tcatttatgc
      661 tgtacccgca acacttacac cctgtttttt tatggcactc acaagtgagt ttggttcaaa
      721 tggtttagta atataatccg tagcacctac caatttaccc ctcattttat caatgattcc
      781 gtctttacct gaaagcatta ttaccggtac ttcttttgta agctcgttac ccttgattat
      841 cttgcaaagt tgatacccat ccatatgggg caagttaata tctagaaaga taatatcagg
      901 tatgaattca tttaactttg ctaacgcctg cattgcattt gatgatactt ctacctggta
      961 accgtgcctg ctaagagtaa cagaaactat tttttgtaca gtagggctgt catcaaccac
     1021 taaaattgta ggtttcgata cagtatctgc tggttgagca tattgatcct gaactggaat
     1081 aaccggctca tttaccggtg gtggtggagg tggtatgtcc ggctctactt ccctaagctc
     1141 aggctttgga ggttcagcac ttactgttgt acctacatga tttccattac tgtcaatggt
     1201 cttattaaac gattcaatat tatcataaga agaatcttca tttagtgatc ttaagtcggt
     1261 ttcattattt acaggtggga caacatttcc aatctcactc tctttctgcc cgggaatttg
     1321 gaagaggtct tccacttctt taccgggatc agcagattgg gtaggggctg tggcccctgg
     1381 ttgagaatca gctgcatcag gtgctaaatc tgtttctgta cctgcacttt ctgtctgcat
     1441 agtgggaatt gtaaaaacat ctttgttatc aggaacttct atctgatcag ttatttcttc
     1501 agcttccagg ctcagttcta ttgtcttatc atcatcctct tcacttgctg attggctgct
     1561 tgaaaatacg tttaactccg ggctgctatc ttgcgcttgt ggaggtacag gtatgttaat
     1621 tggctcagat ttttgtgatt ctacaggctg aaaaccaact gtagccccag cctgttctgc
     1681 aggagcctga aatagactct gttctccacc tgccgtacta gctggttcac tttcagtctg
     1741 ttcactatgc ggccgcgcat ctacttcacg actctcagcc ggcggctgaa aaagattact
     1801 cgagctatca gagctttgta atgatatatt ttcaaagctg ctatccgaac tctgttttac
     1861 ccctcttaca taatcatcat catcttttgc aaatacatca attatttcat tttgatttgg
     1921 tttttcatca gttgcttgtg gttgaggttg tggagattga ccgggaaact gaggctcatc
     1981 aatttgtggt ggttgagctt gagattcagg ctctgtagta agcgatgaag ctagagtatc
     2041 tgcagtttct gttttttctg atgatacctg tggagacgct gtaaaaacag aatgatctgt
     2101 ctgttgagat tgtgatactg gcggctccgg atagctttgt ggttgcgttt caatttgcgg
     2161 ctctggctca ggttgtgctg gttcggacgg tgcttgtgca gcaacttcag gttcaggctc
     2221 tgatggttgt actatttgtt gttcctcagg tttttgaaca atagtttgtt cttgttcagc
     2281 tttttgcaat ggttcaggtt cttgcttttg tacaacctgt tcagcatcaa tgttatgatg
     2341 atttacaagc gcgcttttaa tactactaaa ttctgctata tatggtttta tattgtgtcc
     2401 tgtcaaaaat cttaactcat caatcagtac aatatttgta ggatcaatca ttgcaacact
     2461 caagaatgcc ccttctaaat caaatggaat caggttatta tcgacggccg tgtcctgagg
     2521 gattagcttt atgactgatg gctcaatatt tatcgctgaa aggtctactc tggggaattg
     2581 attgtctata ttagtaagtt caacaagttg agactcggta atataacctt ttctaattaa
     2641 tatttcaccg agtgatttat tattgttctt ttgctcttct acagcttctt ttaggttatc
     2701 aagagataaa taaccctttt caataaacag tttggctagt ttaacattca ttccattgag
     2761 tccttaatat gtgcttaatc tatagttttg ttacactttt tttatatatt tatatatcta
     2821 gcaagaatta tgccaattca ggaaataaaa ttccctataa tatacagtaa attagtatac
     2881 ataattatag taaattataa atttgagcac cccactctaa tttcaaaaac ctgtcttatt
     2941 ataacatcat attaattaga aataaacgct tatataattt cttatccaat aattcagatt
     3001 cgatttcttt aataataaca ataaactttt tatattaatc tgtaatttga actgtttttg
     3061 tataattttt aatacagtct gcttaatttt tatgcattaa aaattttatt taatttacaa
     3121 aatataatga tattagatgt ttaagtaaaa ttcttagatg gtataatctt tgcaattatc
     3181 atctctaatt tacttaggag gcattctgca atgaaaaaaa ttgaagctat tattaaacca
     3241 ttcaaactgg atgacgttaa agaggctctg aaggacatag gaattcaagg tctaacagta
     3301 actgaagtaa aaggttttgg aagacaaaag ggacacacag agctatacag gggtgccgaa
     3361 tatgtaatag attttctacc taagattaag ctggagatta ttgtttccga tgacacagta
     3421 actaaggtag tagacgctat agttgaaagc gcaaggaccg gcaagattgg ggacggaaaa
     3481 atatttattc tacctatgga agaagtaata agaataagaa ccggtgagcg tggggaagac
     3541 gcactataat agattattac agcatttgaa aataacttta taaattacaa ggaggaaggt
     3601 tagatggcaa aatctaccaa aggcggcgct cagagtccgc caaacaatgc aaatgatgtt
     3661 cttaagcttg taaaagatga gaacattcag attatagact ttaggtttct cgattttgtg
     3721 gggatgtggc agcatttcag tacacccaca catgaattgg acaaagaagc attcgaagaa
     3781 ggactgggtt ttgatggatc aagcatcaga ggttggcagt caattcacgc aagcgatatg
     3841 ctaatgatac cggatcccaa aactgccaga attgatccat tcactaatat accaactctc
     3901 gcattaatat gtgatattgt agatccaata acaagagagc cttattcaag agacccgaga
     3961 aacatagcca aaaaagctgt ttcttacctc cagagcacag gaattgctga cacagcttat
     4021 tttggacctg agctggaatt ctttatttta gacgatgtta gatatgacca gacagcaaac
     4081 accggatact attttgtaga ttcagtagaa ggtatctgga actcaggcac ggatgaagga
     4141 ccaaaccttg gttataaacc aagacataaa gaaggttatt tcccctgccc tcctacagac
     4201 agctatcagg acctaagatc cgaaatggtt ttagagatgg aaaatcttgg tatagcagtc
     4261 gaagctcatc accacgaagt atcaacagcg ggtcaggctg agatagacat gcgtttttcc
     4321 cctcttgttg atatgggcga caatatgatg tggtataaat atatttgcag aaatgtagca
     4381 agaaaatatg gtaaaacagt tacatttatg ccaaaggtta tattcgaaga taatggttca
     4441 ggaatgcaca tacatcagtc actctggaaa aaaggcaaac cactttttgc aggaaacagc
     4501 tacgcaggaa tgagcgaaat ggcaatgcat tatattggtg gaatactaaa acatgctaaa
     4561 gctatctgtg ctttttccaa tccaacaaca aactcttaca gaagacttgt accagggttt
     4621 gaagctcctg ttaaccttgc atattcggca agaaacagaa gtgctgcagt aagaattcct
     4681 atgtactctc caagtcctaa agctaaaaga cttgaaacaa gattcccaga cccatcagca
     4741 aacggatacc ttacattctc tgcaatgctt atggctggcc ttgatggaat cgagaacaaa
     4801 attgatccgg gtgatcctct cgataaagac atttactcac ttggacctga ggaactggag
     4861 ggtatccctt atgttccagg ttcgctcgaa gaagctctta actcacttaa agaagatcat
     4921 gaattcctta caaaaggtga cacattcaca gatgatgtag ttgaaacatg gattgactac
     4981 aagatggaaa atgaagttaa tcctgtgaag ctaagaccag tacctcatga gttcatgctt
     5041 tactttgatg tgtaattaat tagagaataa agtttttaga taacaaaaaa aggcggccaa
     5101 acggtcgcct ttttttatgc ctaattcaaa ttttctatta tcggttagca gtctgcaaaa
     5161 atcagaagta gcaaaagtta cacaacaaaa ggttaaaata ccactatgat gaactaaccc
     5221 ctaatataga tcagatgatc atgcaggaaa aattattgat gtacttattg cttagaaagt
     5281 tgaaaatatt atatatagaa taagaataag acccgcagtc ataacagctg cataggctct
     5341 tacaagacca aactgaaact tccttacaaa agttgatagg catgagaaaa agcctgcgat
     5401 tctgtttaca agcccatcaa caatatttga gtcaaaccaa aatgacatct ttgatgactc
     5461 tttaaacccg tctataaatg tatgctcata aacctcgtcc atataccatt tggagttgga
     5521 agcgttgaac agaaagccaa agtcctcatt tgctgtaatc ttgctaagat gtttaggcct
     5581 ataaatatag aaataatagg caagcccaat tcccaaaaca gctgcaacaa tggaaagtat
     5641 aagtagatcc atgtggctaa agaagtgatg actaactgct gatgcatgat gctgttcaag
     5701 aggtatagct gcccagataa actctttaaa cttgtttaca tggtgcagtc caaacatctc
     5761 accaataaaa tccggcacgc caacataacc gcctattact gatagaacag ccagtacaat
     5821 caaaggaatt gtcatagtac ttggagattc atgaagcttt ttcattatat tagcaggaac
     5881 cctgctcttt ccttcaaaag ttaggaaata taggcgcatc atataaagtg cagtgagcat
     5941 tgccgtaaca agtgctactg cccagtgaat cgcaaatcct ttttcgtaca gttcaccaag
     6001 tatctgatct tttgaaaaga agcctgagag aagaggtagg cctgaaattg ctaaagtacc
     6061 tacaagaaaa gtcttggaag ttataggaag atatttccga agccccccca tctttcttac
     6121 atcctgctca tcagacattg catgtattac gctaccagca ccaagaaata aaagtgcttt
     6181 aaagaatgcg tgggttacta agtgaaacat accagcggca taggcgccga ttcccacacc
     6241 catgaacata aaaccaagct ggctcacggt agaatatgcc agtacctttt taatatcggt
     6301 ctggctaagg gcaatcgttg cagcaaaaaa cgcagtaaat acagcaattg ttacaactat
     6361 gaattgcgct acaggagaag cactgaaaaa tacggcacac cttgtaacca tataaacccc
     6421 tgcagttacc atggttgccg catgaataag agcactaacc ggtgttggtc ctgccatggc
     6481 atctggaagc cacacataga gtgggaactg tgccgattta ccaacagctc ctacaaaaag
     6541 cagaagcgct attgctgtga ttgttatagc tgggatatta tttaaaattg cctgacttga
     6601 agcctgagaa aaaacgtctg cataactaag agagccaaaa gttatgaaaa taagaaatat
     6661 cgctatgaga aaaccaaagt cacctattct gtttactata aatgcctttc ttccggcaag
     6721 ggccttctga tcatcatcat accaaaaacc aattaacaga taagaagcaa gcccaactcc
     6781 ttcccagcct acaaacatga gcgggaagtt attaccgagt acaagaacca gcatgaagaa
     6841 aacaaatagg tttaggtaag taaaatatct ggcgtatgat ttatcatggc tcatataccc
     6901 aatcgaatag acatgaatca aaaacccaac ccaagttatt actagagtca taattgctga
     6961 aatattatca aaaagaaaac caaactttat atccagctca ccagatacta tccaggaaaa
     7021 tgaggtttcg actatgccgg ttaatccttc cgacccaaca agagtataga acatatatgt
     7081 agcaataagg gcagaaaaga ggatcgagct gcaggcaata acactaacaa tgcttttttc
     7141 agatttatgg tcttgtttta ttaaacctgt agtaaaaagc aggccgttta tagcagctcc
     7201 aataagtgga agtaatataa ttaaaaatag catttttttg tatttaacct ttaagtaact
     7261 tcagtacatc tatattaata gtctctttat tatgataaat agataccact agagctaaac
     7321 caactgcaac ctcacatgct gcaactacta aagacataag cataaaaacc tgtccagtgt
     7381 tatcgccatg aaacctcgaa aaactcacaa gcgccagatt tgccgcgttt agcattagct
     7441 caagtgacat aaggactata attaaatttt ttcttgtgat tacaccaaaa gcacctatca
     7501 caaagagcat aaagcttagc agaacgtagt ggtttattgt aacttccatc taatctgcgc
     7561 tcctttttga aagtacaatc acaccgatta ttgcaattaa aatcagaacg gatgtaagct
     7621 caaaaggcag tatataataa gtaaaaatgc tggtgcccac gttttcaaca gagccgtaat
     7681 cttctgaaac tgaaatatcg gataacttgg caggttttga ggttccaatc accacatttt
     7741 gcactatcat tgcagttata aatagcactg caagagcaga gcttaccttt agaggaacat
     7801 tctgtgcatc aaaattaagt gattcatccc taaggatcaa aaacatcaca gtaaacatga
     7861 atagcaccat tatggcacct gcataaacga gaatctgcac cacagccaca aactgagctg
     7921 atagcataat ataaagcaca gaggtattaa acagagtaag aacaagtgca actatacttg
     7981 ctacagggtt gcggttaaca accacggcaa tagagcttgc aagcacagca cacgcaaaga
     8041 aataaaataa atatgtgtcc aattatttaa gtcctgatta ttagttccgg aaaaaaattt
     8101 acgttagatt atatatggta tatgcggtag tcaacccttg aattagtttt ttttaggaaa
     8161 ttgcagataa accttattcc aagtcctcta gtgtttgtgc atcaagaaca tctctatctc
     8221 ttccagtcga tcttttattc ttaatcagat cactctttga aataaaagga agcagtaatc
     8281 catccagaac taagtcgtgc ttactcttgt atgcgtcctt aaaactaaca ccatcaatat
     8341 cagttaaaac atcaattctc acaggagcaa cacctatctg atatacaata tctttatttg
     8401 aaaaagtctc aggtgttata tccttaagag gggctccaaa atcagataaa accttgtata
     8461 gcttaactga attttctgaa gagttctcca cccatatatc aaaatcacca gttgaccttg
     8521 tgtaaccata tgcggccatc gcatatgcac ccacaataag gaacttaacc cgactttcta
     8581 ataagctttt caacatctct ttgtaatctt tgttcaacat ttttcctgtc tttgaaatac
     8641 catacctctc ttgttagttc ccacaccatt gatattcttt cttgcggaga cgcatctaca
     8701 aacccactat cattatcttt aagtcgcttg atagcctcaa tatttttctt tagccgcatt
     8761 atattattat atcacagtca gaacaatttt ttcaccccaa ccttcctaca catatcctcc
     8821 attttacaga tatcacattt tggcccgata ggcttgcata ggttttgtcc ataggctaca
     8881 agtagtgagt ttatatcaat ccaatatctc tttggaagtt tttctctgag tgcaaactca
     8941 ctctcatcag gggtttttgt ttttatgtac cccaggcggt taaatattct gtgtacatga
     9001 gtatccacgc atattccagg tttattgtat cctagcgtaa ttaccaggtt tgcagtcttg
     9061 cgccccactc ctttaagttt taaaagctca tcaagatcat ccgggaccct ggattcatat
     9121 ttatctatta attctttgca cacttctttt attgtttttg cctttactct gtaaaaccct
     9181 gccggataaa ttgctttttc gatctgcggg attttgagct tgagcatgtc cttagaattg
     9241 gatgcaagtt ttaatagtct tacagaagcc tcttctgttg ttttgtcctt tgtgcgaaga
     9301 ctaataatgg ttgagataag gacattaaac gccttttttg atttatcagc ttcaacactt
     9361 acaaaaggag tcttaaatct tttatactcc tttttcagga ctttaactac ttcattaata
     9421 tttatatctt ccattaccaa cccagatttg ttattgtgta tttttataag attataagta
     9481 attattaaca cagttatgga atatccaaag ctaagatttg tagatacttt tccaattgag
     9541 catgaagggg gcaaatatgt ttgccttaga gaccctcagg acacaaatgg caatatgctc
     9601 cttgtagccc ctcaggcaat ctatgttatt tcttatttca caggcagtaa ctccatagtg
     9661 gacattcagc taaagattct aaaagagtac ggagaattaa ttgaaaaaga ccatatcata
     9721 cagcttgcta agcagttaga cgatgcgctt cttctagaca gtgacaaata tagagaccac
     9781 cagctgcagc ttgaaaatga ctttaaaaaa tcaaagatta gagaagcaag tcatgcgggt
     9841 atgtcatatc cgaatgaaaa agaagaatta accaaatgga tcga
//
LOCUS       DBNGSV010000317         1693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_333129, whole genome shotgun sequence.
ACCESSION   DBNGSV010000317 DBNGSV010000000
VERSION     DBNGSV010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_333129"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1583
                     /locus_tag="AC159U_11375"
     CDS             join(211..547,547..1583)
                     /locus_tag="AC159U_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611856.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR3337693.1"
                     /translation="MKKKEKREPESIIKEIKRKTRRKFTSEEKIRIVLEGLQGESSIA
                     DVCRREGIHPTLYYKWSKTFLEAGKKRLNGDTVREADSAEVKDLRAENESLKQLVAEL
                     SLKNRVLKKTFEGFGIRINKYMKYSQAEKMEVIRMVEESNMSVKQTLGKIGINRSTFY
                     EWYKKYAELGYDGLAPRYKQSGHIWNAIPEWEKDRVTEVAKEYPERSCREIACLVTDK
                     HGYFISESSVYRILKARDLVSSPAYRVISASETFEHPTKRVHELWQTDFTYFKIINWG
                     WYYLLSVLDDYSRYIIAWKLCTTMKTVDVTTVLEMAFAKTGVIHVHVYKRPRLLSDNG
                     SCFISSELKDYLYGNDMTHIRSKPYHPTTQGKIERYHRSMKNLVLLDNYYSPEKLSAQ
                     IGQWVEYYNNHRYHESLDNVTPRDRYYGTDRELLEQRQITKLKTMKLRRKINKRLAIQ
                     SLTGGMN"
BASE COUNT          572 a          313 c          409 g          399 t
ORIGIN      
        1 agactgatca atgaactcaa tacagatgct tacaaggata aattagatga aatagctaat
       61 ctttctaatc ccttctttga tggagatatt gaagagaaaa aaaatttcaa acaaaggagg
      121 tgatacctgt ttcccgtcag ataaatttgg acaggaagta gaagttccta agctatggaa
      181 agtgtaaaat acaaaccata ggagggacac gtgaaaaaga aggagaagcg agagcctgaa
      241 tcaatcatca aagagatcaa acgaaagacc cgccgcaaat tcacatcaga agagaagatc
      301 aggatagtgc ttgaagggct gcaaggggaa tcaagtatag cagatgtatg tcgccgagag
      361 ggtattcacc caacattgta ttacaagtgg agcaaaacct ttcttgaggc ggggaagaag
      421 cggctcaatg gagacacggt acgggaagcc gattccgcag aggtaaagga tctgcgagct
      481 gagaacgaat cgctgaaaca gttggtagcg gaattaagcc tgaagaaccg ggtgctaaaa
      541 aaaaccttga agggtttgga ataaggataa ataagtatat gaaatactct caagcggaga
      601 agatggaagt aattcgtatg gtagaggagt ccaatatgag cgtaaaacag acccttggca
      661 agatcgggat taaccgaagt acattctatg agtggtataa gaagtatgcg gaacttggct
      721 atgatgggct ggctcctcgt tacaaacagt ctggtcatat ctggaatgcc atcccggaat
      781 gggagaagga tagagtcaca gaggttgcca aagaatatcc tgagaggtct tgccgtgaaa
      841 tcgcctgtct tgttacagat aagcatggat atttcatttc tgaatcgagt gtataccgga
      901 tattgaaagc gcgtgatctt gtcagttcac cggcctatag agtaatttcg gcttctgaga
      961 catttgagca tcctacaaag cgtgtgcatg aactctggca aactgatttt acctatttta
     1021 agattatcaa ttggggctgg tactatctgc tgtcagtgtt ggatgactat tctcgatata
     1081 ttatcgcatg gaagctgtgc acaaccatga aaaccgtcga tgtaacgaca gttctggaaa
     1141 tggcatttgc aaaaacaggg gtaatacacg tacatgtgta taaacgccct cggttgctga
     1201 gcgataacgg gtcatgcttt atctcatcag agctgaaaga ttacctgtat ggtaatgaca
     1261 tgacgcatat acggtcaaaa ccgtatcatc ccactacaca gggaaagatc gagcggtatc
     1321 accgctccat gaagaacctg gtactgctgg ataattacta ttcgccggag aaactctctg
     1381 ctcagatcgg ccagtgggta gaatattaca ataatcatcg atatcatgaa tcgcttgata
     1441 atgtgacacc cagagaccga tattacggca cagacaggga actcttagag cagagacaga
     1501 taaccaaact gaaaactatg aaactcagga gaaaaattaa taaaagattg gcaattcaat
     1561 ccttgaccgg agggatgaat tagactaaac tgaaatcgct aacccatagc tcggacgttt
     1621 gtccaaaacc tttgacgata tacaatcagg tgacctaatt accaatagac atcttgaaag
     1681 taaaaaggca ggg
//
LOCUS       DBNGSV010000318         2741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1165185, whole genome shotgun sequence.
ACCESSION   DBNGSV010000318 DBNGSV010000000
VERSION     DBNGSV010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1165185"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /gene="mraY"
                     /locus_tag="AC159U_11380"
     CDS             <1..288
                     /gene="mraY"
                     /locus_tag="AC159U_11380"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388743.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3337694.1"
                     /translation="GDVGSLALGAAIGSVAVVIKQEILLVIVGGLFVAEVLSVILQVM
                     SFKLTGKRIFKMAPLHHHFELSGWSESKVVIRFWIISLVLALIAFSTLKIR"
     gene            314..1639
                     /gene="murD"
                     /locus_tag="AC159U_11385"
     CDS             314..1639
                     /gene="murD"
                     /locus_tag="AC159U_11385"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3337695.1"
                     /translation="MELKDKKYLVVGLGKTGIVTAKFLLGMGALVAISDSRTDVGYED
                     EIKTFRENGVMVELGGHTNKTFLWPDTIVLSPGVPFTIPFVQTAIENGIEVVSEIELA
                     SKYITKPIIGVTGSNGKTTTTTMIFDILEASGFSVFLGGNIGTPLIEIAGSDSSFDYI
                     LLELSSFQLQGIKEFKPYIAVCLNIYPNHLDHHKDFTEYVESKTKIFANQTENDIAVI
                     NADDPQLKEIPNKLNCRVVTFGKGSYADAYYEDFVVSYEGNDYDLSDLKLVGRHNMEN
                     AMVAIVVGRLLGSTPETIKHSIIKFNSLPHRIEYVREFKNARFFNDSKSTTPHSTLKA
                     LEAFDPPVILIAGGKDKGLDYSVFKDQVTDKVKQLILMGEASSKIKDIYKSSVPLQVV
                     DSLDKAVEQAVESLEQGDTVLFSPACSSFDMFSSYEERGRKFKQIVQCL"
     gene            1623..2735
                     /gene="ftsW"
                     /locus_tag="AC159U_11390"
     CDS             1623..2735
                     /gene="ftsW"
                     /locus_tag="AC159U_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3337696.1"
                     /translation="MFSVFDIKNTRIDLYILLSVLYLISVGLLMVYSTSSIYALDKFN
                     DSTYYLKRHLFYLGAGLLVMTVVANINYTALKKIAFFLYFAGLIFLVLVLFPQIGKEV
                     GGARRWIDLGFFSFQPTEVTKYFLVVYLAHLFSKNREKPQSFSATIVPYLIITGLYIV
                     LMMMQPDFGMAVILITLFGCMMFIAEINFKYIFGLGFTSLLFMGFAVMSADYRMNRIF
                     SFIDPWKDPLGQGYQTVQSFVAFCLGGLTGSGLGEGTQKLYYLPQVHTDFIFSLVGEE
                     LGFIGVLSVIIAFIVLIVRGFKTAANAPDFFGFFLAFGCILLIALQAGINMAVSVGVF
                     PTKGLTLPFISYGGSSLMSSLGAIGLVLSVSKKRIT"
BASE COUNT          803 a          453 c          624 g          861 t
ORIGIN      
        1 ggtgatgtcg gatcgcttgc gctgggagct gcgattggct ctgtggcagt tgtaataaag
       61 caggagattc tgcttgtaat agtaggcggt ctgtttgtgg ccgaggtgct ttctgtaatt
      121 ttacaggtca tgtcttttaa gcttaccggt aaaagaattt ttaagatggc acctctgcac
      181 catcactttg agctcagtgg gtggtcggaa agcaaggtgg taataaggtt ttggattata
      241 tcacttgtgc tggctctaat agcattttca acattaaaaa taaggtagcg gattctgata
      301 ttaaaagaaa ataatggaac ttaaagataa aaaatatttg gtagtcggcc ttggaaagac
      361 aggtattgtt actgctaagt tcttgcttgg aatgggggct ttagttgcaa taagcgacag
      421 caggaccgat gtgggttacg aagatgaaat taaaacgttt agagaaaacg gagtgatggt
      481 agagctaggc gggcatacga ataaaacatt tttgtggcca gatacaattg tacttagccc
      541 cggtgtaccg tttactattc cttttgtaca aacagccatt gaaaatggta tagaagtagt
      601 aagcgaaatt gagcttgcct caaaatacat aacaaagcct atcattggcg ttacggggtc
      661 aaatggtaaa acgacaacca ctacaatgat ctttgacata ttagaggcgt cgggttttag
      721 tgtattcctt ggaggcaata ttggcactcc tctaattgaa atcgccggta gtgacagttc
      781 gttcgattac atacttttag agcttagcag ttttcagcta cagggaataa aagagtttaa
      841 accatatatt gcagtgtgtt taaatattta tccaaatcat ctggatcatc ataaagactt
      901 tacagagtat gtagaatcaa aaactaaaat atttgctaat caaaccgaaa acgatattgc
      961 cgtaattaat gcggatgatc ctcagctaaa agaaatccct aataaattaa attgccgggt
     1021 agtcacattt ggaaaaggca gttatgcgga tgcgtactat gaagattttg tggttagcta
     1081 tgaagggaat gattatgatt tgagtgacct taagcttgta ggcagacaca atatggaaaa
     1141 tgcaatggtc gcaattgttg taggaaggct gcttggatct actccggaga ccataaagca
     1201 ctcaatcata aaatttaatt ccctgccaca caggattgaa tatgtaaggg aatttaaaaa
     1261 tgcaagattt tttaatgatt caaagtccac cactccacac tctacgctaa aagcgctcga
     1321 agcgtttgat ccaccggtaa ttctcatagc aggtggaaaa gataaggggc ttgattactc
     1381 agtgttcaaa gatcaggtta cagataaagt taaacagctt attttaatgg gtgaagcaag
     1441 ctcaaaaata aaagatattt acaaaagctc tgtaccgtta caagtagtag attctttaga
     1501 taaagccgtg gagcaggcgg ttgagtcttt agagcagggt gacacagtgc tcttttcacc
     1561 ggcgtgttct agttttgata tgtttagttc ttatgaagaa agagggagga aatttaagca
     1621 aattgttcag tgtctttgat ataaaaaata caagaataga tttatatatt ctactgtcag
     1681 ttttgtacct catatcagtt gggcttctca tggtatatag tacaagctct atctatgcgc
     1741 ttgataagtt taatgattcc acctactacc tgaaaaggca tttattctac ctgggggcag
     1801 gactgctggt tatgacggtt gtagctaata ttaactatac tgcgcttaaa aagatagcat
     1861 tttttctgta ttttgcaggg ctgatattct tggttttggt actgtttccg cagattggaa
     1921 aagaagtagg tggtgcaaga aggtggatcg accttgggtt ttttagcttt cagcctacag
     1981 aagttactaa atattttctt gtagtctacc tggcacatct tttttctaag aacagggaaa
     2041 agcctcagtc gttttcggca acaattgttc cctatctgat tatcacagga ctttatatag
     2101 ttcttatgat gatgcagccg gattttggaa tggcagttat attgatcaca ctctttggct
     2161 gtatgatgtt tatagctgaa ataaatttta agtacatatt cggtctggga tttacttcgc
     2221 tcttatttat gggatttgca gtgatgtcgg cagattacag gatgaacaga atcttttcat
     2281 tcatagatcc atggaaagat cctctgggac aggggtatca aacagtacag tcgtttgtag
     2341 ctttttgtct tggaggtcta acgggttcag gccttggaga aggaacacaa aaactttact
     2401 acctgcctca ggtgcatact gattttatat tctcgttggt aggagaagaa cttggtttta
     2461 taggagtgct atctgtaata atcgcattta ttgtgctaat agtgcgtggt tttaaaacag
     2521 ctgccaatgc tccggacttt tttggattct ttctggcatt tggctgtatt ttactcatag
     2581 cacttcaggc tggcatcaac atggccgtgt cagtaggcgt tttccctacc aagggtctta
     2641 ctctgccatt tattagctac ggagggtcat cactcatgag cagccttggc gctattgggc
     2701 tagtcttgag tgtttctaaa aaaagaatca catgagagtg a
//
LOCUS       DBNGSV010000319         5461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_819926, whole genome shotgun sequence.
ACCESSION   DBNGSV010000319 DBNGSV010000000
VERSION     DBNGSV010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_819926"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="AC159U_11395"
     CDS             <1..1219
                     /locus_tag="AC159U_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3337697.1"
                     /translation="AVIELPSPVDGSISDLHVKEGETVKIGQLLVTFYSNGDEQSTKN
                     TKQQESVTEESSDDRPIEEEIKVDKKSKETTEPQDDVIENTESDNNIIEINNEGETPT
                     KSNIAQMVPASPSVRRLAREIGVDVSRVQGTGPGGRISEEDVKNFAKSNLSELEAEQT
                     SMSITTNISASQKKGDSIEQMSKVRVLTAQRLTESWKAPHVTQHDKADITELEKIRKQ
                     YGDKVKQAGGKLTMTSILTKISAQALIKYPNFNCSIDMDNLQILNNDEFNIGIAVDTE
                     RGLLVPVIKNADKKNITQISVEMTDISERSRNKKIKPDELQGSTFTITNLGGIGGTYF
                     TPILNHPNVAILGISRALMEPVYIDDEFKPRLMMPLSLSYDHRIIDGADAIKFLRWII
                     EALENPFFTLLDS"
     gene            1238..2659
                     /gene="lpdA"
                     /locus_tag="AC159U_11400"
     CDS             1238..2659
                     /gene="lpdA"
                     /locus_tag="AC159U_11400"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3337698.1"
                     /translation="MTDNNINTELVVIGAGPGGYPAAFHAADLGINVTLIDSREYPGG
                     VCNYEGCIPSKALLHISKLIDEASNASSLGITYKNPDIDLNTLRKWKNEVIKKLTSGL
                     GQLSKFRKVNYIQGYAQFLDSNNLVIHKDDEELTLNYKKAVIATGSSPANIPGIEIDN
                     KTIMDSTGALEIEDIPKSLLIVGGGYIGLELGTVYQALGTEVTVVEMMDSLMTGADQD
                     LVRVLKKRLTSKFKSILLNTRVEIIEKQKGRIKATFKTNKDESIEEMFDKVLVSVGRK
                     PNSQNIGLENTNVKINDRGFIEINDKRQTDDPNIYAIGDVAGDPMLAHKATYEGKIVA
                     EIIAGKNVIYDPYAIPAVVFTDPEIAWCGVTEEEAKEKGLDYTSTRFNWAASGRATTL
                     DRNDGLTKLIIDNKTQRILGMGIVGVGAGEMIAEGVHAIEMGATASDLKLTIHPHPTL
                     TETIMEAAELFFGSSTHVYRKKQ"
     gene            complement(2733..3458)
                     /locus_tag="AC159U_11405"
     CDS             complement(2733..3458)
                     /locus_tag="AC159U_11405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3337699.1"
                     /translation="MMKKLDGKTAIITGGSGGLGKSVVNIFLEHGANVICTYHKEEEL
                     KLFTPLMDNYPNSVVIVKGNVTKEKSIVKIVNNAVKRFKTIDILINIVGGFTKGDITE
                     TSIYDFEAMIDMNLKSTFICSKSVIPQMIVQKHGKIINVAAKPAINGASGVSAYAASK
                     AGVLNLTKSLAEEVLKHNINVNAIIPGTIDTEANRLAMPKAKHKNWVKPEEIAKVMMF
                     LATEDSDPISGAAIPVYGKSYLS"
     gene            3586..3930
                     /locus_tag="AC159U_11410"
     CDS             3586..3930
                     /locus_tag="AC159U_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337700.1"
                     /translation="MAIDLFISMKAETIQEHLFIKCNMMQMPTAQTNPFFIAEFREDE
                     PDKLELLFKFEDLLRNKLINDNGYIIERTDIGIKIKKKVDEKKDYMEEWNEWTEFSNM
                     IFNQLKEVLEEE"
     gene            4013..>5461
                     /locus_tag="AC159U_11415"
     CDS             4013..>5461
                     /locus_tag="AC159U_11415"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3337701.1"
                     /translation="MSEDKTVEYKNTLNLPKTDFPMKANLAQREPELLKKWYKNNLYS
                     KIRDFSKGKDKFILHDGPPYANGHIHLGHCLNKVLKDIIIKSQTLFGKDCYYVPGWDC
                     HGLPIEYQVTKKLGSKKADKTKSEIRKLCREYAKRFIDIQRDEFKRLGVFADWDNPYL
                     TMNYEYEANIAKELGKFMENGGLYRGKKPVYWCYSCQTALAEAEVEYEDKTSESVYVK
                     FKLLSTIFTSQYPELKGRDVYAVIWTTTPWTLPANQGLAFHPEFKYVAVEHNGEILIV
                     ADELKEAVLGENLNVLETIKGDKFQSLKFQHPFYDRESLGVNADYVTLEAGTGIVHIA
                     PGHGQDDYEVSKLYDLDVYAPVNEKGVFTDDVKFFDGQFVYKANENIIAKLEELGTLI
                     KHEEIRHSYPHCWRCKSPIIFRATPQWFISMAANDLRVNSLAEIDRVKWIPDWGKERI
                     FNMIQNRPDWCVSRQRSWGVPRVVFYCTDCDTE"
BASE COUNT         1958 a          899 c         1027 g         1577 t
ORIGIN      
        1 agctgttatt gagcttcctt cacctgtaga tggttcgata tctgatttac atgtaaagga
       61 aggtgaaacc gtaaaaattg ggcagcttct cgttactttt tactcaaatg gagatgaaca
      121 atcgacaaaa aataccaaac aacaagaatc tgtaacagaa gaatccagtg atgatagacc
      181 catagaagaa gaaataaaag ttgataaaaa atctaaagaa accactgaac ctcaagatga
      241 tgtaatagaa aatacagaaa gtgataataa cataatagag ataaataacg aaggagagac
      301 tccaacaaaa tccaatattg ctcaaatggt tcctgcctct ccatcggtaa gaagacttgc
      361 aagagaaatt ggtgttgatg tatccagggt acagggcacc ggccctggag gaagaataag
      421 tgaagaggat gtgaaaaatt ttgctaaatc caacctatct gaattagaag cagaacaaac
      481 atctatgtca ataactacaa atattagtgc ttcgcagaaa aagggtgatt caatagaaca
      541 gatgtccaag gttagagtac ttacggcgca aagacttaca gaatcatgga aagcgcctca
      601 tgttacccag catgataagg ctgacattac cgagcttgaa aagataagaa agcaatacgg
      661 ggacaaagtc aaacaagcgg gtggcaaact taccatgact tcaattttaa caaaaatctc
      721 tgctcaggcg cttattaaat atccaaattt taactgcagc attgatatgg ataacctcca
      781 aattctaaat aatgacgagt ttaatattgg aattgcagtt gatacagaga gaggcctact
      841 agtacctgta ataaaaaatg cagacaaaaa gaacataact caaatatctg ttgaaatgac
      901 agatatatca gagcgttcca gaaacaagaa aatcaagccc gacgaactac agggtagcac
      961 ttttactata acaaacttag gcggtatagg gggcacatat tttactccaa tactgaatca
     1021 tcctaatgta gcaatattgg gcatatcaag agcacttatg gagcctgtat atatagatga
     1081 tgaattcaag cccagactca tgatgcccct gtctctatca tatgatcata gaataataga
     1141 cggcgctgat gccataaaat ttttaaggtg gattattgaa gctttagaaa atccgttttt
     1201 cactttgttg gattcataaa ttagttgagg aatcaaattg actgataata atataaatac
     1261 tgaattagta gtaattggtg caggtccagg aggatacccg gctgcatttc atgctgctga
     1321 tcttggaatt aatgtaaccc tgattgactc tagagagtat cctggtggag tttgtaacta
     1381 tgaaggatgt ataccatcaa aagctttact tcatatatct aaacttatag atgaagcttc
     1441 taatgcttct tcgctgggta tcacatacaa aaatcctgat attgatttaa acactttaag
     1501 aaaatggaaa aatgaagtta ttaagaaatt aacatctgga ctcggacaac tttcaaaatt
     1561 cagaaaagta aactacattc aaggatatgc ccagttttta gactctaaca atttagttat
     1621 acacaaagat gatgaagagc ttacccttaa ttacaaaaaa gccgtaatag caacaggctc
     1681 atctcctgca aatattccag gaattgaaat cgataataaa acgataatgg attcaacagg
     1741 cgccttggaa atagaagata tacctaagag tctgctcatt gtaggtggtg gatatatcgg
     1801 tctcgaacta ggcacagttt atcaggcttt gggtacagaa gtaaccgtag ttgaaatgat
     1861 ggatagctta atgaccggag cagatcagga tttagtaaga gttcttaaaa aaagattaac
     1921 atcaaaattc aagtcaatcc ttctaaacac tagagtagaa ataatagaaa aacaaaaggg
     1981 aagaattaag gcaactttta aaacaaataa agatgaatct atagaagaaa tgttcgataa
     2041 agttttagtt tctgttggaa gaaaaccaaa ttcacaaaac ataggattag aaaataccaa
     2101 cgtaaaaatt aatgatagag gatttataga aattaatgat aaaagacaga ctgatgatcc
     2161 taatatttat gctataggtg atgtagccgg tgatccaatg cttgcacata aagcaacata
     2221 tgagggaaag atcgtggccg aaataattgc aggtaaaaac gtaatttatg atccctatgc
     2281 aatacctgct gtagtcttta ccgatccaga aatcgcatgg tgcggagtaa ccgaagaaga
     2341 ggctaaagag aaaggacttg actatacttc aacaagattt aactgggcag cttccggaag
     2401 agctactact cttgatagga atgatggtct tacaaagtta ataatagata acaaaacaca
     2461 aagaatactg ggaatgggaa ttgtaggagt aggagcagga gagatgatag ctgaaggggt
     2521 acacgcaata gaaatgggag caactgcttc tgacttaaaa ctcactatac atccacatcc
     2581 aaccttaaca gaaacgatta tggaggctgc tgaactattc tttgggtcat caactcatgt
     2641 ttacagaaaa aaacaatgag taaatgatgt gattactcga cattgagaaa agcatgaatg
     2701 ttctcctgag tgtcaagtct tatacatata atttaactta aatatgactt cccataaaca
     2761 ggtattgctg ccccgctaat aggatcagaa tcctctgttg caaggaacat cattacttta
     2821 gcaatctctt cgggttttac ccagtttttg tgttttgctt taggcatagc cagtctattg
     2881 gcctctgtat ctatagtccc gggaataatt gcgtttacgt ttatattatg tttaagaact
     2941 tcttctgcta atgatttagt aaggtttagc actcctgctt ttgaagctgc ataagcagat
     3001 actcctgatg ctccatttat agccggtttt gcagctacat taattatctt tccatgcttt
     3061 tgaactatca tctgtggaat taccgattta gagcaaataa aagtagattt aaggttcata
     3121 tcaatcattg cttcaaaatc atatatactt gtctcagtta tatccccctt ggtaaaacca
     3181 cctactatgt ttattaaaat atctatagtt ttaaatcgtt ttactgcatt attaactatc
     3241 tttactatag atttttcttt tgtaacattt cccttaacaa ttactacgga gtttgggtaa
     3301 ttatccataa gaggagtaaa taattttaat tcttcttctt tgtgataagt acaaattaca
     3361 tttgctccat gttctaagaa aatattaact acgctttttc caagtccgcc cgaaccaccg
     3421 gttataattg cagtttttcc atcaagtttt ttcatcatac actcctttgt tgtaatactt
     3481 gtatgtataa atattagtag aattatatct tatttttaca attacaataa cttgacagct
     3541 caagtaaatt tagccttaat aaacagttag aaagggatta aatatatggc aattgatctt
     3601 tttatttcta tgaaggctga aactattcag gagcatttgt ttatcaaatg caacatgatg
     3661 cagatgccca cggcacagac caatcctttt tttattgccg aattcagaga agatgaaccg
     3721 gataagttag aacttctttt taaatttgaa gatcttctaa gaaataaact tataaatgac
     3781 aacgggtata ttattgaaag aacagatatt ggaataaaaa ttaaaaagaa agtagatgag
     3841 aaaaaggatt acatggaaga gtggaatgaa tggacagagt tttccaatat gatatttaat
     3901 cagttaaaag aagtgttgga agaagagtag ctattaatac ttaatacttg aattaaacac
     3961 ttactcaatt aatatcttat ccttgatttt attttagtta tctaaatcca ctatgtctga
     4021 agataaaact gtagaatata agaatacatt aaatctgcct aaaacggatt tccctatgaa
     4081 ggcaaacctc gctcagcgcg agcctgagtt gttaaaaaaa tggtacaaaa acaaccttta
     4141 ttccaagatt agagattttt caaagggaaa agataaattc attcttcatg acggcccgcc
     4201 ttacgctaac ggacatatac atttaggaca ctgcctaaac aaggtattaa aagacataat
     4261 cataaagtct cagactcttt ttggaaagga ttgctattat gttccaggat gggactgcca
     4321 cgggctacca attgaatatc aggttacaaa gaaattagga tctaaaaagg ctgataaaac
     4381 aaaatcggaa ataaggaaac tttgcagaga atatgcaaag agatttatag acatacaaag
     4441 agatgaattt aaaaggttag gtgtttttgc cgactgggat aatccctatc tcactatgaa
     4501 ttatgagtat gaagctaata ttgcaaaaga gcttggaaaa ttcatggaaa acggcggtct
     4561 ttacagaggg aaaaagcctg tttactggtg ctattcctgt cagactgcgc ttgccgaagc
     4621 agaggtagaa tatgaagata aaacatctga atctgtttat gtaaaattta aactactttc
     4681 aacaattttt acttctcagt atcctgaact caagggccgg gacgtatatg ctgtgatctg
     4741 gacaaccact ccatggacac ttccagctaa ccagggtctt gcattccacc ccgaatttaa
     4801 atatgtagca gttgagcata atggagaaat attaatagta gcagatgaat taaaagaagc
     4861 tgtacttggt gagaatttaa atgtattaga aacaatcaaa ggcgacaaat ttcagtcact
     4921 taaatttcag catccatttt atgacagaga atcacttggt gtaaacgcag attatgtaac
     4981 tctcgaagct ggtacaggca ttgtacatat agcccccggt catggtcagg atgattatga
     5041 agtatcaaaa ctatatgatc tggacgttta cgcacctgtt aatgaaaagg gcgtatttac
     5101 agatgacgta aaattctttg atggacagtt tgtatataaa gctaatgaaa atattattgc
     5161 caaacttgaa gaactgggaa ctttgattaa gcatgaagaa ataagacatt cctatcctca
     5221 ttgttggaga tgcaagagtc ctataatatt cagagccact cctcagtggt ttatatcaat
     5281 ggctgcaaat gatctaagag ttaattcact tgctgaaatt gacagagtca aatggatacc
     5341 cgattggggt aaagaacgta tttttaacat gatacaaaac aggcctgact ggtgtgtatc
     5401 aagacagcgc tcgtgggggg tacccagagt tgtattttat tgtactgact gcgacacaga
     5461 a
//
LOCUS       DBNGSV010000320         7181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1928515, whole genome shotgun sequence.
ACCESSION   DBNGSV010000320 DBNGSV010000000
VERSION     DBNGSV010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1928515"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..550
                     /locus_tag="AC159U_11420"
     CDS             221..550
                     /locus_tag="AC159U_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3337702.1"
                     /translation="MVTAGTPTTLAAIDLELDYYDADRVNGHRLKKKSILSILNRLCR
                     LSSDQRLNIKGLFKGREDLIIPGTLIIMELLTIFDENNIIVSDGGLLEGLIYSKAHKL
                     CNNSMQL"
     gene            601..1644
                     /gene="hrcA"
                     /locus_tag="AC159U_11425"
     CDS             601..1644
                     /gene="hrcA"
                     /locus_tag="AC159U_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3337703.1"
                     /translation="MNQITLTNKDKSILSAVVDYYIRTGNPVGSKTLSESYNLKWSTA
                     TIRNTMAKFMDCGYLSQPHISAGRIPTDKAFELYINSLMDLKKLSQDKIGFINMRYKN
                     VLGTMDDLLFETSSVLSDISEFAGLATLPDSKYLEIKSAKLVKIDKKKILLIIIFNGG
                     ITEKTLINLKNEITEQTLKHLSYYLNGISEHMTLDDLKTTVLSELKFKKDEIYNEIVR
                     SILKLTDPTQSDKSSSDIYINGRESIIDKINFNNTQDIKDLIKAFEEKKYLIEILSNV
                     KNGDGIKVFIGSEHGMISGFSLVACSYGNNERLGTIGVFGLMRMDYSKIIPLVDYTAK
                     MVSNIVRDGGYYD"
     gene            1637..2524
                     /gene="grpE"
                     /locus_tag="AC159U_11430"
     CDS             1637..2524
                     /gene="grpE"
                     /locus_tag="AC159U_11430"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3337704.1"
                     /translation="MTDDDSKDKEDEQISINGNTESMDTDNLPDEDPNEKIKALQKNL
                     DDKNSSYDDLYERYLRIAADFDNYKKRMSKEKTDLVTYGNEELIKALLNVIDNLERAL
                     EHSGSEGSSESIIKGVDLVYRQFISCLEKFGLGRIDANEGTKFDPNYHQAVEHVETDD
                     LTPGLIVSEMIKGYLLKERLLRPSMVSVSKGVVNKPFKENKSKEVKREEEIQTQQSPD
                     SSDTQELELDMTDTEFNLDDLKIDNDFTPNTKNNSTEQGETLNNSDEEISIEVNIDSD
                     NDNKDDILELIEEDIKENN"
     gene            2537..4375
                     /gene="dnaK"
                     /locus_tag="AC159U_11435"
     CDS             2537..4375
                     /gene="dnaK"
                     /locus_tag="AC159U_11435"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3337705.1"
                     /translation="MPIVGIDLGTTNSCVSIIEGGSPVVIPNDEGSRITPSVVAFTDD
                     GSRFAGAVAKRQAVVNPRSTIFGVKRLVGRKINSPEVDKAKEFAPYEIISNENEDAWV
                     EVNDKQYSPQEISSIILMKMKNIAEEYLGYEVDEAVITVPAHFNDSQRQATKDAGRIA
                     GFNVRRIINEPTAAALAYGFDKKENQKVAVFDLGGGTFDISILEINNGVFEVKATSGD
                     TFLGGDDFDQKLINLVIEDFQNQHSIDLKTDKMALQRIREASEKAKHELSSLLETNIN
                     LPFVAVDTSGPQHLNIKINRDQFEQMVADLIDRLDEPCLQALQDAKLDASEIDEVVLV
                     GGMTRMPKVSDKVRQLFNKDPQTRINPDEVVAIGAAVQGGILEGQIDEVILLDVVPLS
                     LGVETRGSLCTKIIERNTTIPTKRSRIFTTALDNQDFVNIHVLQGEREMADDNISLAK
                     LILIGIPPAPRGIPQIEVSFEVDADGILNVSARDLGTGKKQAIQVETSGGLSEGQIKL
                     IIEEGKKHVEEDKMKKEFADIKNEAEGLLFSVDKTLTSYSDKAEEGFIDLVRDARDKM
                     KEALSSQDFDKIKTALETLKERSYAFAEIIYSKKENKTEYQQDTDS"
     gene            4408..5517
                     /gene="dnaJ"
                     /locus_tag="AC159U_11440"
     CDS             4408..5517
                     /gene="dnaJ"
                     /locus_tag="AC159U_11440"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3337706.1"
                     /translation="MSKRDYYEVLGVGRDASSEQIKRAFKKLAFEYHPDRNPDDKGNE
                     DKFKEINEAYQILSDENKRAQYDSFGHLSGEGFQSNQNFSNFGDLFGSLFEDVFTGGG
                     SRTKAQRGRDLKYDLELMFEEAVFGIDKDIEIPKHSTCSTCSGTGAQSGGQTTCGTCS
                     GSGSMNYSQGFLTINRTCANCQGSGYIVTKPCKSCEGNGLVFENKKVKVKVPAGVETG
                     SRLRMRGEGESGYNGGPSGDLFIELHVKEHEFFKRHDNNIILEVPISFVHAAMGTKIE
                     IPTIEGKEEFKFPAGTQPGQSFKIKGKGIADLRSGRKGDMYVVSQVEIPKKITSKQKE
                     LLKEFEKLSKAEDQPLVSKFISKFQNYIEKIVVFN"
     gene            complement(5514..5789)
                     /locus_tag="AC159U_11445"
     CDS             complement(5514..5789)
                     /locus_tag="AC159U_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3337707.1"
                     /translation="MPSVRIPTPLRRLTQEKDEVDINASNVAELIEQLESQFPGIKDR
                     LCDEKGEVRRFINLYVNNEDIRFLEGTETSLKSEDIVSIIPAIAGGY"
     gene            complement(5777..7045)
                     /gene="thrC"
                     /locus_tag="AC159U_11450"
     CDS             complement(5777..7045)
                     /gene="thrC"
                     /locus_tag="AC159U_11450"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3337708.1"
                     /translation="MSCVKGLICRECGEQYPKEAIHVCEYCFGPLEVEYDYKKISGII
                     SRDLIQKRDHNIWRYKELLPIDDEPTVGTSVGYTPLIKADNLASVLGVKELYIKNDAV
                     CYPTLSFKDRVVSVALSKAKEFGFETVSCASTGNLANAVSALSTSAGLKSFIFIPKNL
                     EQSKIVGTSIYGTNLVEINGHYDDVNRLCSEIAGSFGWGFVNINLRPYYAEGSKSFAF
                     EFMEQLGWKVPDNVIIPMASGSLLTKIWKAIHEFQKVGIIDDYSTKIFGAQAKGCSPI
                     STAVKNKWDTFKPQKPDTIAKSISIGNPADGYYAMKAIRDSGGWSEDVTDPQIIEGIK
                     LLAETEGIFTETAGGVTVAVTKKLIENNHIDKDESILISITGNGLKTQEPLIDEIVMP
                     HTINADIEEFKQLYEDISNNTKENIKKCLP"
BASE COUNT         2583 a         1081 c         1371 g         2146 t
ORIGIN      
        1 gagagattac aacgagtaca gacctcattc ggcacttgat aatatgacgc cggaggagta
       61 tgttaaacaa cacataaaaa agccggaaat ctctaattat cccggtacag cttttgggtg
      121 agggtcagaa gtaagaacaa aaatatcaga tcatattgtt aaattaatta atagagagct
      181 taatattaaa gaatttaata aaattaaaga taaggaaatt atagttacag caggtacacc
      241 tacaacatta gctgctattg atttagaatt agattattat gatgctgacc gagtaaatgg
      301 tcatagacta aagaaaaaga gtattttatc tatactcaat agactctgta ggctaagttc
      361 tgatcaaaga ttaaatatta agggtttatt taagggtagg gaagatctta ttatccctgg
      421 aacattaata ataatggaac ttttaacgat ttttgatgag aacaatatta tagtgagtga
      481 cggtggatta cttgaaggat taatatacag taaagcgcat aaattgtgta ataattcaat
      541 gcagttataa ttcattgata atatataagc aagaaattat attttcaatt gagtatatat
      601 atgaaccaaa ttacattaac taataaagat aagtctatat tgagtgcagt tgtagattat
      661 tatattagaa ccggaaatcc cgtaggctca aaaacacttt cagaatctta caacctaaaa
      721 tggagtacag ccactattag aaataccatg gctaaattca tggattgtgg atatttatcc
      781 cagccgcata tatctgcagg aagaatacca acagataagg cgtttgagct gtatattaac
      841 tctcttatgg atttaaagaa actatcacaa gataaaatag gatttattaa tatgcgttat
      901 aaaaatgtat tgggtactat ggatgattta ttatttgaaa ccagcagtgt actgtctgat
      961 atatctgagt ttgcagggct ggctacattg ccagattcta aatatctgga aattaaatca
     1021 gccaaacttg ttaaaataga taaaaagaaa atacttttaa ttattatctt caatggtgga
     1081 ataacagaaa agactcttat taatttaaaa aatgagataa cagaacaaac attaaagcac
     1141 cttagttatt acctaaacgg tatttcagaa catatgaccc ttgacgatct taagactacg
     1201 gttttatctg aactaaaatt taaaaaagac gaaatttata atgaaatagt acgtagcata
     1261 ctcaaactca ctgatcctac acaatctgat aaaagttctt ctgatattta tattaacgga
     1321 cgcgaatcta ttattgataa aattaatttt aataacaccc aggatatcaa ggaccttata
     1381 aaagcttttg aggaaaaaaa atatttaata gaaatattaa gtaatgtaaa aaacggtgac
     1441 ggaataaagg tttttattgg cagtgaacat ggtatgataa gtggttttag tcttgttgcc
     1501 tgttcatatg gtaataatga gagactaggt actattggag tttttggttt aatgagaatg
     1561 gattattcaa aaattatccc actagttgat tatacagcca agatggtgag taatattgtt
     1621 agagacggag gatattatga ctgatgatga tagtaaagat aaagaagatg aacaaatcag
     1681 cataaatggc aatactgaat ctatggatac agataatctg ccggatgaag accctaatga
     1741 aaaaataaaa gctcttcaga aaaatttaga tgataaaaat agttcttacg atgatcttta
     1801 tgaaaggtat ttacgcattg ctgcagattt tgataattac aaaaaaagaa tgtctaaaga
     1861 aaagacagat cttgtaactt atggaaatga agaacttata aaagcacttt taaacgtaat
     1921 agacaaccta gaaagggcac ttgagcattc cggctcagag ggatcttcag aatcaataat
     1981 taagggagtt gatttggttt acaggcaatt tataagctgc cttgaaaagt ttggtctagg
     2041 gagaattgat gctaatgagg gaacaaaatt tgatcctaat tatcatcagg ctgtagagca
     2101 tgtagaaact gacgatttaa ctccagggtt aattgtcagt gaaatgatta aaggctattt
     2161 acttaaagag cgtcttttaa gaccatcgat ggtatcagta tcaaaaggag tcgtaaataa
     2221 gccttttaaa gaaaataaat caaaagaagt aaaaagagaa gaagaaatac aaacacagca
     2281 aagccctgat tccagtgata ctcaagaatt agaattggat atgactgata ctgaatttaa
     2341 tcttgatgat ttaaaaattg ataatgattt tactccaaat actaaaaata acagtactga
     2401 acaaggtgaa actttaaata attccgatga agaaattagc attgaagtta atatagacag
     2461 tgacaatgat aacaaagatg atatattaga gttaattgaa gaagatataa aagaaaataa
     2521 ttaggagaac taataaatgc cgattgttgg tattgatctt ggaactacca attcatgtgt
     2581 ttcaataatt gaaggcggaa gccctgttgt tatacccaat gatgagggaa gcagaattac
     2641 tccctccgta gtcgctttta cagatgatgg aagcagattt gcaggagcag tagctaaaag
     2701 acaggctgtg gtaaacccaa gaagcacaat atttggtgtt aaaagattag taggcaggaa
     2761 aataaatagc cccgaagtcg ataaagccaa agaatttgca ccctatgaaa ttataagcaa
     2821 tgaaaatgaa gatgcctggg tagaggttaa tgataagcag tatagccctc aggaaatctc
     2881 atctatcata cttatgaaaa tgaagaatat tgctgaggaa taccttggat acgaagttga
     2941 cgaagctgta ataaccgtac cggcacattt taatgattct cagagacagg ctacaaaaga
     3001 tgccggcaga atagccggat ttaatgtaag aagaataata aacgagccta cggcagcagc
     3061 cttagcatat ggatttgata aaaaggaaaa ccaaaaagtt gcagtatttg atttgggtgg
     3121 cggcacattt gatatctcga ttctcgaaat aaacaacggg gtatttgagg taaaagctac
     3181 cagtggtgac acgtttttag gtggagatga ctttgatcag aagctaataa atttggttat
     3241 agaagatttt caaaatcagc attcaataga tttaaaaacg gacaaaatgg cgcttcaaag
     3301 aataagagaa gcttcagaaa aagccaagca cgaactatct tcacttcttg aaactaatat
     3361 caatctgcca tttgtagcag tggatacttc aggtcctcaa catctgaata ttaagattaa
     3421 tagagatcaa tttgagcaaa tggtggcaga tctcatagat agattggacg aaccttgtct
     3481 acaagcttta caagacgcta aactcgacgc atcagagata gacgaagttg ttttagttgg
     3541 tggtatgaca agaatgccca aagtttcaga taaagtacgt cagttattta acaaagatcc
     3601 acagaccaga ataaacccag atgaagttgt tgcaatagga gcagcagtgc agggtggtat
     3661 acttgaaggt caaatagatg aagtcatact cttggatgtt gtaccattat cacttggagt
     3721 cgaaacaaga ggcagccttt gtactaaaat tattgaacga aatacaacta ttcctacaaa
     3781 aagaagcaga atattcacta cagccctgga caatcaggat tttgtaaaca ttcatgttct
     3841 gcagggtgaa agagaaatgg ctgatgacaa tatttcatta gcaaaactta ttctcattgg
     3901 aataccccct gcaccaagag ggatacctca aattgaagta tcttttgaag tagatgcaga
     3961 tggaatactt aatgtatcag caagagatct tggaactggg aaaaaacagg caatacaggt
     4021 tgaaacttca ggtggactgt ctgaaggcca gattaaatta ataattgaag aaggcaaaaa
     4081 gcacgttgaa gaagataaga tgaaaaagga atttgccgat attaaaaatg aggcagaagg
     4141 cttgttgttc tctgtcgata agacgcttac atcttattct gataaagcag aagaaggttt
     4201 tatagattta gttagagacg caagagacaa gatgaaagaa gccctgagtt ctcaggattt
     4261 tgataaaata aagactgcat tggaaacact aaaggaacgt tcttatgcat ttgcagaaat
     4321 aatatattct aaaaaagaaa acaaaactga atatcagcag gataccgata gttaaaaaca
     4381 gaagtagcta ctttctaaat ataaattatg tctaaaagag attattatga agttttgggt
     4441 gttggtagag atgcatcctc tgaacaaata aaaagagctt ttaaaaaact tgcgtttgaa
     4501 tatcatcccg atagaaatcc agatgataaa ggtaacgaag ataagtttaa ggaaataaat
     4561 gaggcttatc aaatactcag cgatgaaaac aaacgcgcac aatatgacag ttttggtcat
     4621 ttatctgggg agggtttcca atcaaatcag aatttttcta attttggaga tctgtttgga
     4681 tcactttttg aagatgtatt tacaggtggt ggcagcagaa caaaggctca aagaggtaga
     4741 gacctgaaat atgatttaga gcttatgttt gaagaggctg tttttggtat agataaagat
     4801 atagaaatac caaaacacag tacttgctca acgtgcagcg gtacaggagc acaatctggt
     4861 ggtcaaacaa catgtggtac atgctcaggt tcaggttcaa tgaactattc acagggcttt
     4921 cttacaatta acaggacttg cgctaattgt cagggatcag gatatatagt tacgaaacca
     4981 tgtaagagct gtgaaggaaa cggtcttgtt ttcgaaaaca agaaagtaaa agttaaggtt
     5041 cccgcaggag ttgaaacagg gtcgagacta aggatgagag gtgagggtga gtcaggatac
     5101 aatggtggac ccagtggaga tttatttatt gaacttcatg taaaagaaca cgaatttttt
     5161 aaaagacatg ataacaatat cattttggaa gtaccaatta gctttgttca tgcagctatg
     5221 ggaacaaaaa ttgaaattcc caccattgag ggtaaagaag agtttaaatt tccggcagga
     5281 acacagcctg gacaatcatt taaaataaaa ggtaagggta ttgcagattt aagatctggt
     5341 cgtaagggcg atatgtatgt ggtatctcag gttgaaatac caaagaaaat aacttccaaa
     5401 caaaaggaac tgctaaaaga gtttgaaaaa ctctctaagg cagaagacca gcctctagtt
     5461 agtaaattta tatctaaatt ccagaattat atagaaaaaa tagtggtatt taactaatat
     5521 ccacctgcaa ttgccggaat aatagaaacg atatcttcag atttaagtga tgtttcggtg
     5581 ccttctaaaa atcttatatc ctcattatta acatataggt taataaacct tctaacttca
     5641 cctttttcat cacataatct atcttttatt cccgggaact gactttcgag ctgttctatt
     5701 agctctgcta cattggaagc attgatatct acttcgtcct tttcctgagt taatcttcta
     5761 agtggtgttg gtattcttac ggaaggcatt ttttaatgtt ctccttagtg ttatttgaga
     5821 tatcttcgta caactgctta aactcttcta tatctgcatt tattgtatgc ggcataacaa
     5881 tttcgtcaat aagcggttcc tgagttttaa gaccattacc tgtaattgat attaatattg
     5941 attcatcttt atctatatga ttattttcta taagcttttt agtaactgca actgttactc
     6001 ctcctgcagt ttcagtaaat ataccttcag tttctgcaag aagtttaata ccctctataa
     6061 tctgaggatc tgtaacatcc tctgaccagc ctccgctgtc tctgatagct ttcattgcat
     6121 aataaccatc agctggattg cctatagata ttgatttagc tatagtatct ggtttttgag
     6181 gtttgaatgt gtcccattta ttttttacag cagtactaat gggagaacag ccttttgcct
     6241 gagcaccaaa tatttttgta gaataatcat ctatgattcc aactttttga aattcgtgta
     6301 tggctttcca aattttagta agaagcgagc cgcttgccat tggaattatt acgttatccg
     6361 gtactttcca tccaagttgt tccataaatt caaaagcaaa agatttggaa ccctcggcat
     6421 agtatggtct taggttaata tttacaaatc cccagccaaa acttcccgct atttcactgc
     6481 ataatctgtt tacatcgtcg tagtggccgt ttatttcaac caagtttgta ccatatatag
     6541 aggtaccaac aatcttggat tgttctaggt tcttgggtat aaaaataaaa cttttaagtc
     6601 ctgcagaagt tgataatgca gatactgcat tagctagatt acctgtagaa gcgcatgaaa
     6661 ctgtttcaaa tccgaattct ttagcttttg atagtgcaac agataccact ctgtctttaa
     6721 atgacagggt agggtaacaa actgcatcat tttttatata taattcctta acccctagta
     6781 ctgaagccag attatcagct tttattagcg gagtatatcc tacagaagta ccaactgtgg
     6841 gttcatcgtc aattgggagt aactctttgt atctccagat gttatgatct cttttttgga
     6901 ttaagtctct tgatatgatt cctgagatct ttttatagtc atattcgact tcgagtggac
     6961 caaagcaata ttcacatacg tgaatagcct ctttgggata ttgctctcca cactctctgc
     7021 atataagtcc ttttacacaa ctcatttctt agctccaaaa aagaatgtaa taattactat
     7081 ttattataat gatgtcaaga atataatacc agaaataatt aagaattagg ggagattaaa
     7141 taaaattatg aatggaatta ttgaactact taagacactt a
//
LOCUS       DBNGSV010000321         1373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1028151, whole genome shotgun sequence.
ACCESSION   DBNGSV010000321 DBNGSV010000000
VERSION     DBNGSV010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1028151"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC159U_11455"
     CDS             <1..374
                     /locus_tag="AC159U_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337709.1"
                     /translation="HADEWNCPATAAEHFDSKLKALEGHCEDIGRNIKDIALSQQNVC
                     IIVDNKKDLADKLSFGKKRYGFFGDIEKFGIIGTPDNCIKKINENYEKGIKKYTIFFA
                     DIMNHDTLNYFAKEVMSEFRD"
     gene            complement(385..1224)
                     /locus_tag="AC159U_11460"
     CDS             complement(385..1224)
                     /locus_tag="AC159U_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3337710.1"
                     /translation="MPPRTTRKRTKTTAKNKPNNSFKNIIFEKKRKVAYVTINRPEVR
                     NALNSDTREELAIAIEDAWLDDKIGVIVLTGAGGKAFSAGGDLSWIPDPKKKVDARYM
                     HVHYRLATAIRNCGKPVIARVDGYCIGGGNELNMLCDLTIASDTSVFAQAGPLVGSAP
                     VWYGLQQLQHSVGDKKAREIVYLCNRYTAKEAEEMGWINKAVPKEDLDKEVGIWCKRL
                     LEMSPQSLRIAKFQINFNSDIAHSQVSHGLELARFFMKAPEMVEGASAFMEKRKPDFW
                     KVK"
BASE COUNT          388 a          292 c          238 g          455 t
ORIGIN      
        1 aacatgccga tgaatggaac tgccctgcta cggctgctga gcattttgat tctaaactta
       61 aggctctgga aggtcactgt gaggatattg gaagaaatat taaggatatt gcactctctc
      121 agcaaaacgt ctgtattata gtagacaata aaaaggatct tgcagacaaa ttgtcattcg
      181 gcaagaagag atatggattt tttggtgata tagaaaaatt tggaattata ggtacacccg
      241 ataattgcat taaaaaaatt aacgagaact atgaaaaggg aataaaaaaa tacactatat
      301 tctttgcaga catcatgaac cacgatactc taaactactt tgctaaagaa gtgatgtcag
      361 aatttagaga ttgatatagc tatattactt taccttccag aaatctggct ttctcttttc
      421 cataaatgcc gaggctccct ctaccatctc aggggccttc atgaaaaacc gtgcgagttc
      481 aagtccgtgg cttacctgag aatgcgctat gtctgagttg aaattaatct gaaattttgc
      541 tattcgcagt gactgagggc tcatctctaa aagcctctta caccatatac ctacttcttt
      601 atcgagatct tcttttggta ctgccttatt tatccacccc atttcttcag cctcttttgc
      661 agtataacgg ttacatagat aaacaatttc tcttgccttt ttatccccaa cagagtgctg
      721 cagttgctgt agaccgtacc ataccggagc actcccgacc agggggcccg cctgagcaaa
      781 tactgatgta tctgaggcta ttgttaaatc acatagcata ttaagttcat taccgccccc
      841 aatacagtaa ccgtcaaccc tggctataac cggcttacca cagtttctta tagcggttgc
      901 aagcctgtaa tgcacatgca tataccttgc atcaactttt ttctttggat caggtatcca
      961 tgagagatca ccgccggcag aaaatgcctt gcctccggct cctgtaagga ctataactcc
     1021 tattttatca tccagccatg catcctctat agctattgca agctcttccc ttgtatcaga
     1081 gtttagtgca tttctaactt caggcctgtt tattgttaca taggcaactt ttctcttttt
     1141 ttcaaatata atgttcttaa agctattatt tggtttgttt ttagctgtag ttttagttct
     1201 ttttctcgta gtcctaggcg gcatatacac cctccagatt tatagaattc ttaaataact
     1261 attaatagtg ttcaagattg tttttctaat tttgcattct cttcaatctt tttagcaaaa
     1321 tcctgggcaa aatgagcaat catatcggct aattctttat tctttgtaga ttt
//
LOCUS       DBNGSV010000322         6957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1720560, whole genome shotgun sequence.
ACCESSION   DBNGSV010000322 DBNGSV010000000
VERSION     DBNGSV010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1720560"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1901
                     /locus_tag="AC159U_11465"
     CDS             51..1901
                     /locus_tag="AC159U_11465"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337711.1"
                     /translation="MSSKKNIRELIPSLIHIFKYFSAYIGKQKTIIALSTLALFAQVG
                     FRLLEPWPIKYIFDYLLLNDLSKENTITGYFKNFDNNSVLIILCVSLIIFTALRSIAN
                     YYSTIGFAVVGNRVMVKIRERLFDHLQKLSLRFHDKSKSGDLTLRLISDVGILRDVTV
                     TAVLPFIGNILIITGILSVMLFMHWKLTLVSIMLLPFFWLRTKTLSRRLHNVSRDQRR
                     REGAMAATAAESINAIKTVQALFLHNKFLNIFSTQSQKGFKDSVKAKKIEANLGRSVD
                     IIVAASTAIVLWYGVRLVFLHEITPGDILVFVTYLKAAFKPIRDTVKYTGRLSRAVAS
                     GERVLDVLEVSPDIVDTPDSIEAPDFKGNIKFDKVSFSYSDKVDTIKNISFKINPGQF
                     IALVGPSGNGKSTILSLLMRLYDPNQGTVCVDGEDIRKYKIDSLRSQITVLLQDNILF
                     AESIKDNISGGLKYVCHEQIVNAATLANAHEFISTMPNGYDTIVGERGTTLSNGQRQR
                     IAIARAILRDTPILILDEPTASLDENNEAKVIQAIEHLRKNRTTILVTHKLKQALKAD
                     QIFYIKDGEILDCGNHNSMVVSCNDYKKQYYLQNPAENDLKNKIYAVPNR"
     gene            1882..4242
                     /locus_tag="AC159U_11470"
     CDS             1882..4242
                     /locus_tag="AC159U_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3337712.1"
                     /translation="MLSQIDKELTLRDNSLPGLRLLLDTEYIQNRINTHLPYLKIKKV
                     KNTYLNYKPSNKCIVLHNLISDSGSLLAYSTVYGLQKIDNERKRLENRISDSKHKRSQ
                     GVELTREFNNFDNGIITYFLPVDRRLKNIVKLYDTEYRNKLFSYKLPQNQDLIDSEIV
                     PINYKPEKRFVAKLSKNSKEASVIKLYGDSYYEHAAINSRLYKNNSAFFVPNLQKRIS
                     KLNCLVYEWVDGKNLNSLILNNNPQANIVENVGTALGELNNQKITKQLKFDRKYFIYK
                     LLSISQDVSYLLPNISVKINRIVNRVSELLLNIPDIYIPIHGDFNADQVIVGSRESTG
                     DSSLCLLDFDQSCLGHPAFDIGNFIAHYINQSYKISLNSSLVTEIRESIENGFFKTSK
                     IRIDADDINIFTVACLIRLLTDPFKYRYGDWDNSIYDLLEYIKQTTDKLKNKNTFYVS
                     SEDNYIEIDDSHSAASDSRMPYMKYALDPEYMQSLFDTHMLSNSQPKPKISSIEVIRY
                     KKERRCLLEYKFRNNRNLCLIGKTKARGSVKSNYKLNDALYNSGFDEKSDDFISIPKP
                     VFYNKNLNMWFQRKVDGDNYRNLLLSTNGLEYTKLIARSAHKIHKCNIDSGKTHTIDN
                     EIEILEDRLSKVSVSKPTLSGRINNTLLAAKNLASSIADPVLCSIHRDFYHDQLIING
                     NRIYLLDFDLYCKGDPAVDIGNFLAHLTELSIRTNGNADGYLDIENEIEKQYLSISDL
                     ICPDSIQAYKTLTLLRHIYISTLFVDRCIYTEQILNLCEQRLQDYL"
     gene            4297..6033
                     /locus_tag="AC159U_11475"
     CDS             4297..6033
                     /locus_tag="AC159U_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR3337713.1"
                     /translation="MDIPDYLRKIVFIFLILISNNHAFGIEQSNIINKIDHTQNSTSN
                     IYTIYLSGNTNYNVGKVPENKNEPYRVYIDFKDSKLGKQIPHTLDTSDSLVKRIRSAQ
                     FNTNTARVVFDLSERHDPKITRLSKPSRLEISFENNSDKNRKPKVYAASNDKNDSADN
                     IKASGISQTDFGEETLNNQFNGENGDEPFDPDAKPPLPIKIGDKISVGGKVNLKFDIR
                     NNSEDLDSNIGDTLSRFETQVSAAGLINPLPNIDIYAEGRFTDLQLIEDEFDVGEEKT
                     DVELRRAYIHWRDFTEQHFDLQVGRQRFKDDREWIYDDNLDAFRVYYKPGKFSAELSV
                     SSILIDLEDDRDKTVNYVLYSQYEYDKKEMFALFGVVRQDRDGIDDTYTFGASWRGRP
                     LNNRNKIWIDNAVFIGRNRDKEWKQGYGVDAGFSKSFKLPLRPNFTIASAFGSKDFRQ
                     TGLEDNNARFKGSTNFKYYGEVFDPELSNLFITTLGIGIKPHKKKSLDLVYHYFYQVE
                     KRDRLVSPDIEPDPTGESRDIGHEIDLILGASITEYVKVAFVGGLFFPGSAFEEDDLS
                     YFGEIELQFAIK"
     gene            6135..6479
                     /locus_tag="AC159U_11480"
     CDS             6135..6479
                     /locus_tag="AC159U_11480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3337714.1"
                     /translation="MSDKERREHPRILVDEKVTFSTNHPINDGYTYNLSPNGLSLLSD
                     VALPVDASICINIHLTKYDSENMLVDEVVTVEGKVVWVTEHPNQQAKMGIKFNRPNAE
                     LLKYYMKNSAEL"
     gene            6539..6898
                     /locus_tag="AC159U_11485"
     CDS             6539..6898
                     /locus_tag="AC159U_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3337715.1"
                     /translation="MSDIKIKRIYDKPSVEDGKRILVDRLWPRGLSKDKARVDHWEKD
                     IAPSHELRKWFNHTPDKWVEFKKRYFRELDSINLDHFLSIISNSKTTLMFSAKETKYN
                     NAAALKEYLENINKAHK"
BASE COUNT         2512 a         1156 c         1276 g         2013 t
ORIGIN      
        1 gaattattga tatagtaaaa aaattaaatt agcaggctaa taagcaataa atgtctagta
       61 agaaaaatat aagagagctt attccaagct taatacacat atttaaatat ttttctgcat
      121 atataggaaa gcagaaaact ataattgcat tatccactct ggcactgttt gctcaggtgg
      181 gtttcaggct tctcgaaccc tggcccataa aatatatatt cgattatctg ctactaaatg
      241 atttaagtaa agaaaatact ataaccggtt atttcaaaaa ctttgataac aactcagttc
      301 ttatcatact ttgtgtttcg cttataatat ttacagccct taggtctatt gccaattact
      361 acagcactat tggttttgcg gtagttggca acagggtaat ggtaaaaata agagagagac
      421 tttttgatca cctccaaaag ctctcactta gatttcatga caaaagcaaa agcggagacc
      481 tgactttaag actaataagc gacgttggaa tactaagaga tgtaacagtc accgccgtac
      541 tgccttttat tggaaatatc ctgattataa ccggaatcct gtcggttatg ctgtttatgc
      601 actggaagct cactcttgtt tctataatgc tgctcccttt tttttggtta agaacaaaaa
      661 ctcttagcag gcgcctgcac aatgtatcac gcgatcaaag aagaagagag ggagcaatgg
      721 ctgccactgc tgcagagtct attaacgcca ttaaaaccgt acaggccctc tttttgcaca
      781 ataagttttt aaacatattt tctacacaaa gccaaaaagg atttaaagac agtgttaagg
      841 caaaaaagat tgaagctaat cttggaaggt ctgtggatat catagttgct gcaagcaccg
      901 ctattgtgct ttggtatgga gtaagactgg tatttctgca tgaaattacc ccgggggaca
      961 tattggtctt tgtaacttat ctaaaagccg cattcaaacc cataagggat acagtgaaat
     1021 atacaggcag gctatcaaga gcggtagcat caggcgaaag agtactagat gttctggaag
     1081 ttagtcctga tattgtggat acccccgatt ccatagaagc tccggatttt aaggggaata
     1141 taaagtttga taaagttagt ttctcttatt cagataaggt tgatacgata aaaaacatat
     1201 catttaaaat caaccccgga cagtttatag ctcttgtagg accgtctgga aacggcaaat
     1261 ctacgatact tagtctgctt atgaggctgt atgatccaaa tcagggaact gtatgtgttg
     1321 atggtgaaga tattaggaaa tacaagattg attcgttacg ctcacagata actgttcttc
     1381 tacaggacaa tatattattt gctgaaagta ttaaggataa tatttctggc ggcttaaaat
     1441 atgtatgcca tgaacagata gttaacgcag ctacacttgc taacgctcat gaatttatta
     1501 gcactatgcc aaatggttat gacacaattg ttggcgagag gggtacaacg ctttcaaatg
     1561 ggcagagaca aagaattgca attgcaagag caatactcag agatactcct atactaatcc
     1621 tagatgagcc cacagcatca cttgatgaaa acaatgaagc aaaagtaata caggccatag
     1681 aacacctgag aaaaaacaga actacaattt tggtaacaca taaattaaag caggcattaa
     1741 aagctgatca gattttttat ataaaagacg gagaaatatt agattgtgga aatcataaca
     1801 gtatggttgt ttcatgtaat gattataaaa agcaatacta cctgcaaaat ccagcagaaa
     1861 atgatttaaa aaacaagata tatgctgtcc caaatagata aagaattaac cttaagagac
     1921 aactctcttc caggtttaag attgttactt gacactgaat acattcagaa cagaataaat
     1981 acgcatttac cttatctgaa aattaaaaag gtaaaaaaca cttacttaaa ctataaacct
     2041 tcaaacaaat gcatcgttct tcataatctt atctccgact ctggaagttt acttgcctat
     2101 tccacagtct atggtttaca aaaaattgat aatgaacgaa aaaggcttga aaatagaatt
     2161 tccgattcca aacacaaacg gtcacagggt gttgaattaa ctcgtgaatt taacaacttc
     2221 gataacggca taataaccta ttttcttcct gtagaccgga ggcttaaaaa tattgtaaag
     2281 ctctatgata cagagtatag aaataaatta ttcagttaca agctgcctca aaatcaagac
     2341 ctgattgatt cagaaatagt tccaataaac tataaacctg agaaacgatt tgttgctaaa
     2401 ctttccaaga atagcaagga agcttctgtt ataaagcttt atggagattc ttattatgaa
     2461 catgccgcta ttaactccag attatataaa aataattctg cattctttgt cccaaacctg
     2521 caaaaaagaa ttagcaaact gaattgcctg gtttatgagt gggttgacgg caaaaattta
     2581 aatagtctta tattaaataa taaccctcag gcaaatattg ttgaaaatgt gggtacagca
     2641 cttggagagc ttaacaatca gaaaataaca aagcagttaa aatttgacag gaaatatttt
     2701 atttacaaac tgctctcaat ttcacaggat gtatcgtatc ttcttcctaa catatcagta
     2761 aaaataaaca ggatagttaa cagagtatca gagctgcttt taaatatccc ggatatttac
     2821 atacctattc atggtgattt taatgcagat caggtaattg tgggcagcag ggaaagtact
     2881 ggagatagtt cattgtgctt attggatttt gatcagtcat gccttggcca cccggctttt
     2941 gatattggaa attttattgc acattatatt aatcagtcat ataaaataag cctaaattcg
     3001 agtttggtta cagagataag agaatctata gaaaacggtt tttttaaaac atctaaaatc
     3061 agaatagatg ccgatgatat aaatattttt acagttgcat gcctgataag acttcttacc
     3121 gatccgttta aatacagata tggagactgg gataatagta tttacgatct tctggaatat
     3181 ataaaacaga caaccgataa gcttaagaat aaaaatacat tttatgttag ttcagaagat
     3241 aattatatag agatagatga cagccattca gcagcaagcg atagcagaat gccttacatg
     3301 aaatacgctc ttgatcctga gtatatgcag tcattgtttg atacacatat gctctccaac
     3361 agtcaaccaa aaccaaaaat atcaagtata gaggttataa gatataaaaa agaaagaaga
     3421 tgcttgttgg aatataaatt tcgcaacaac aggaatctat gcttaattgg aaagactaag
     3481 gcaagagggt cagttaaaag taattataag ttaaatgatg cattatacaa ttctggcttc
     3541 gatgaaaagt ctgatgattt tatttctata cctaaacccg tattctataa caaaaacttg
     3601 aatatgtggt ttcaaagaaa ggtagatgga gataattaca gaaacctgct attaagcaca
     3661 aatgggcttg aatatacaaa gttaattgcc aggtccgcac ataaaattca caaatgcaat
     3721 attgatagcg gaaaaactca tacaatagat aacgagatag aaattctaga agacagactg
     3781 agtaaggtgt cggttagcaa acctacactt tcaggccgga taaacaatac tcttttagca
     3841 gctaagaatc tggcatcatc catagctgat ccggtactat gcagtattca tagagatttt
     3901 tatcatgacc agcttattat aaacggcaac agaatatatt tattagactt cgatctgtac
     3961 tgcaaagggg accccgctgt tgatataggt aatttccttg ctcatttaac agagctaagt
     4021 ataagaacca atgggaatgc ggacgggtat ctagatatag aaaatgaaat agaaaagcaa
     4081 tacttaagca tttcagatct tatctgtcca gactcaatac aagcctataa gactcttact
     4141 cttctaagac atatttatat aagcaccctt tttgtagaca gatgtattta tactgaacaa
     4201 attctcaacc tatgtgaaca aagactacaa gattatttat aaaacactaa cagtaaagta
     4261 cagggaggtt gataatttac tattaaaaaa aataaaatgg acatacccga ttatttaaga
     4321 aaaatagttt tcatattctt aatcctgata tctaacaacc acgctttcgg tattgagcaa
     4381 tcaaatatta ttaataagat agatcatacc cagaacagta cgagcaatat ttatactata
     4441 tatttatcag gcaacaccaa ttacaatgtt ggtaaagtac ccgaaaacaa aaatgagcca
     4501 tacagggttt atatcgattt taaagattca aaacttggta aacaaatacc tcatacatta
     4561 gacacatcag attcattagt aaagcgtata cgctcagcac agtttaatac caatacagca
     4621 agagtagtat ttgatctttc agagaggcat gatccgaaaa ttacgcgtct tagtaaaccc
     4681 agccgcctgg aaatatcgtt tgaaaataat agtgataaaa accgaaagcc taaagtgtat
     4741 gcagcaagca atgataaaaa tgattccgct gacaatatta aagcttcggg catttcacaa
     4801 actgattttg gagaagaaac acttaataat caattcaatg gcgagaatgg tgacgagcct
     4861 tttgaccctg atgcaaaacc cccgctccca ataaagattg gcgataaaat atcagtgggc
     4921 ggtaaagtta atctaaaatt tgatatcagg aataacagcg aagatcttga tagtaatatt
     4981 ggagatactc tatcaagatt cgaaactcag gtaagcgctg ctgggcttat caacccccta
     5041 ccaaacatcg atatttatgc tgaaggaaga tttacggacc tacaattaat tgaagatgaa
     5101 tttgatgtcg gagaggaaaa aacagatgtt gagcttcgca gagcatatat tcactggaga
     5161 gattttacag aacagcattt tgatttgcag gtaggaaggc agaggtttaa ggatgacagg
     5221 gagtggatat acgacgataa cctggatgca tttagagtgt attataagcc aggaaaattt
     5281 agtgccgagc tatctgtaag ttcgatacta atagatttag aggacgatcg ggataaaaca
     5341 gtaaattatg ttctttattc ccaatatgaa tatgataaaa aagaaatgtt tgcactgttt
     5401 ggtgtagtaa gacaggatag agacggaata gacgatacat atacctttgg cgcttcatgg
     5461 agaggcagac ctttaaacaa caggaataaa atctggattg ataatgctgt ttttattggt
     5521 agaaatagag acaaggaatg gaaacagggt tatggagttg acgcagggtt tagtaaatca
     5581 ttcaaacttc cgcttaggcc aaatttcacc attgcctcag catttggctc taaagacttc
     5641 aggcaaactg ggcttgaaga taataatgcg agatttaaag gatctactaa ctttaaatac
     5701 tatggcgagg tttttgaccc tgagctttct aatttattta taactacact tgggatagga
     5761 attaaacctc ataagaaaaa atcattagat cttgtttatc attattttta ccaggtcgag
     5821 aaaagagaca ggcttgtaag tcctgatatt gagcctgatc ccacgggtga aagcagagac
     5881 ataggacatg agattgattt aatcctcggc gcaagtatca cagaatatgt aaaggtagca
     5941 ttcgtaggcg gtctattttt ccccggcagt gcatttgaag aggatgatct atcatatttt
     6001 ggcgaaatag agcttcagtt tgcgattaaa taacaaacga cttacaaacc tgcatcaatt
     6061 tattaactaa acatatagaa ttattcacaa taaagtatta taataagtaa ccattttaga
     6121 agaatgagta cattatgtct gataaagaaa gaagagagca tccaagaatt ttagtagatg
     6181 aaaaagtcac attttcgacc aaccatccta taaatgacgg atacacctat aacctctctc
     6241 caaatggtct ttctcttctc tccgatgttg cccttccagt agacgcgtcc atatgtatta
     6301 atattcacct taccaaatat gattcggaaa acatgttagt ggacgaagtt gttacagtgg
     6361 aaggcaaagt agtatgggtt accgaacacc ctaatcagca ggccaagatg ggcattaagt
     6421 ttaacagacc aaatgcagaa ctattaaaat attatatgaa aaattctgca gaactataaa
     6481 ctttttatct atgtttttgt tcccctgata ttattacact aatttgatcc agcttgttat
     6541 gtccgacatc aaaatcaaga gaatttatga taaaccatcg gtagaggacg gcaaaagaat
     6601 ccttgtagac aggctatggc ccagaggatt atctaaagac aaggccaggg tggatcactg
     6661 ggaaaaagat atcgcacctt ctcatgaatt aagaaaatgg tttaatcaca ccccggataa
     6721 atgggtggaa tttaagaaaa gatattttcg tgagcttgat agtattaatc tggatcattt
     6781 cctcagtatt atatcaaatt caaagactac tcttatgttc agcgcaaagg aaacaaagta
     6841 taataatgca gcagcattaa aggaatattt agaaaatatt aataaagcac acaaatagtc
     6901 tgtaattttt atttttatta tgtaaaataa cgatccttat taattagtat ggtgggt
//
LOCUS       DBNGSV010000323         4940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_404769, whole genome shotgun sequence.
ACCESSION   DBNGSV010000323 DBNGSV010000000
VERSION     DBNGSV010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_404769"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="AC159U_11490"
     CDS             complement(<1..1015)
                     /locus_tag="AC159U_11490"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3337716.1"
                     /translation="MKIASREQVREIDRSAIEDLQIAGLILMENAGRSVAELIEKEYS
                     QAKNIAIFCGIGNNGGDGFVVARHLISKGFNTQTYICGDEKKYKGDALSNFKSLKNIN
                     AEIINLEEKLPLYLECDLIVDCIFGTGLDREVKGFYEKIIEYLNRRDTPILSVDIPSG
                     LDANSGLPHGCCIKADKTLTFALPKTGTVIHPGVDYCGDLYLSGITTPKELEDNINIE
                     LITASNIKPLLSKRNRDTHKGTYGHLFIIAGSPGKSGASVLSALGAQRIGAGLTTVGI
                     PKSLNPVIEEKLTEAMTEPLAETKNSTLGSGSLKQAVNILSDNKSALAIGPGISTSKQ
                     TESF"
     gene            complement(1144..2040)
                     /locus_tag="AC159U_11495"
     CDS             complement(1144..2040)
                     /locus_tag="AC159U_11495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3337717.1"
                     /translation="MRIILPYLLILFFITSCISNADSNGKYLFLITIDGLRPDAVTEQ
                     TTPNLAAFLKNSSYSLNAQTVYPSNTMPSHTSLFTGLDAKNHKKTLNDHREIKKLLKQ
                     GKYLQFDTVFTIGEKNGFSTTFICGKDKLKFLIRPSFQNTVLCHDIYDKKDNIISIIT
                     TTFSSTLKSKKNNINFIHYPEPDLSGHRHGWMTEKYLESLRIVDKEIEKLLGLIRTEW
                     AEKEYLLIITADHGGTEKTHGTRLKEHMTIPWIAYGSSVKKGYKIQKEVKIYDTVPTI
                     LNFLDINIPPNLDGKAVTEIFN"
     gene            complement(2106..2387)
                     /locus_tag="AC159U_11500"
     CDS             complement(2106..2387)
                     /locus_tag="AC159U_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337718.1"
                     /translation="MSKRTVKIDLFHCDYKDKDGQRCDMEGERSQIKECALCGTDLCS
                     RHYQILSVSNNNMRYLTYFFCEAHSEEFINTLISTFGDTSPVEGGGMAK"
     gene            2578..3546
                     /locus_tag="AC159U_11505"
     CDS             2578..3546
                     /locus_tag="AC159U_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3337719.1"
                     /translation="MIPMKDCVPGVAKFLYMKAARRPKLVNLEITKLCNAGCDFCDYW
                     QTKHEERLTDYGPVIEKINPLAVVITGGEPMLRKDLVDIVKQVKGSTFYVFTSMVTKG
                     DLLTVEKAHDLFNAGLDQIAVSLDFIGKTHDKYRGIDGLWEYLEDLLPKLGKEFKGKK
                     SVIINTIIMDDNLDQIVDLVHQAKQWNIGVSFSSYSVMKTNNESHYIDTNLQKVTEVV
                     DELIRLRKELKGTILTTEYYLREIPGYFKRGGVPDCTAGLSHIQVTPSGHLKRCSEMP
                     VSAHYSEYSPDLYQKTNCDACWFSCRGETQSPANVQRALEYLGVWV"
     gene            complement(3543..>4940)
                     /gene="cysS"
                     /locus_tag="AC159U_11510"
     CDS             complement(3543..>4940)
                     /gene="cysS"
                     /locus_tag="AC159U_11510"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3337720.1"
                     /translation="KKETLVPKDENIIRMYVCGPTVYDSAHLGHARAALTFDIIRRFL
                     KHLQYDVNYVRNVTDIDDKIIERANSLGIAATEVSERYTKEYQEDMAALGILPPDHEP
                     KVSDHIHDIINLIETLINKDVAYVSGNDVFFSIDKFIGYGKLSRRTPDDMIAGSRIDV
                     NENKENPLDFALWKGAKEGEPFWESPWGNGRPGWHIECSTMSMKYLGQDFEIHGGGKD
                     LIFPHHENEIAQSEAATDEQFAKYWIHNGLIQIDKEKMSKSIGNIINVREALKRWSKE
                     AIRLFFLSHHYRNPADFSQQNLDDAEASLQRLYLTLQRIKNIKTIKVNEDEELKKHVE
                     KFRESWESAMQDDFNTAGALGNLFDFCRVINKSIDSEGGSPTLDDAVSEIRLLGTTFG
                     ILEQDPDEYLQKEKLSKSESDISEEEINDLIEQRNSARAEKNWQRSDEIRDYLNSKGI
                     VIEDSSEGTIWRVKS"
BASE COUNT         1360 a          948 c          942 g         1690 t
ORIGIN      
        1 ggaaagattc agtttgcttt gatgtagaaa ttccgggtcc tattgcaagg gctgatttgt
       61 tatctgataa aatatttact gcctgcttta gacttccact tccaagtgtg ctgtttttag
      121 tttcagcaag gggttctgtc atggcttcgg tgagcttttc ttctataaca gggtttaagc
      181 ttttaggtat tccaactgta gtaagtcctg caccaatcct ctgagcccct agtgctgata
      241 gcactgaagc gccggatttc ccgggagagc ctgcaataat aaaaaggtgg ccataagttc
      301 ccttatgggt atccctgttc cttttactta aaagcggctt aatattgctg gctgtaataa
      361 gctctatgtt aatgttatct tctaattctt ttggagtcgt tatacccgat aggtataaat
      421 ctccgcagta atccactcct ggatggataa cagtgcctgt ttttggcagg gcaaaagtta
      481 gggttttatc tgctttaata caacatccat ggggtagtcc tgagttggca tctagccctg
      541 agggtatatc aactgatagt attggtgtgt ctcttctatt taaatattct attatttttt
      601 cgtaaaatcc ctttacttct ctatcaaggc ccgtaccgaa gatgcagtcc acaatgaggt
      661 cacattcaag atacagagga agtttttctt caagattgat aatctcggca ttaatatttt
      721 ttagagattt aaagtttgaa agagcatcac ccttatactt cttttcgtct ccacaaatat
      781 aggtttgagt gttaaaaccc tttgaaatta aatgtcgggc gactacaaat ccatcgcctc
      841 cgttgtttcc tataccacaa aatatggcga tgtttttagc ctgtgaatat tctttttcaa
      901 taagttcggc tacactcctg ccggcgtttt ccataagtat gagcccagca atctgaagat
      961 cttctattgc cgaccggtca atttctctaa cctgttctct ggatgctatt ttcatattta
     1021 tttaacaaaa tatgcaatga tttcattatg ttactactaa taccttacag aatatataaa
     1081 agttgttgat aatgcaatca tagatttatg attgcagtta gatatggcgt gtaacctatt
     1141 gtattaattg aatatttctg tgactgcttt gccgtccaga ttaggaggta tattaatatc
     1201 caggaagttt aagatcgtgg ggactgtgtc ataaatttta acttcttttt gtatcttata
     1261 acctttttta acagaacttc cataggcaat ccatggaatg gtcatatgct ctttgagtct
     1321 tgttccatgg gttttttctg tgcctccatg atccgctgtg ataatcaata aatattcttt
     1381 ttctgcccat tcagtcctta tcaaaccaag aagcttttca atttccttat caacaattct
     1441 taaggactct aaatatttct ccgtcatcca cccatgtcta tggccgctca gatcgggctc
     1501 agggtagtga ataaaattta tattattctt tttagacttt aatgttgaac taaaagttgt
     1561 agtaataatt gagataatat tatctttttt atcataaata tcgtgacata aaacagtgtt
     1621 ttggaagctt ggtctgatta gaaatttaag tttatccttg ccacatataa atgtagtact
     1681 gaaaccattt ttttcaccta tggtaaagac tgtatcaaac tgcagatatt ttccctgctt
     1741 gagaagcttt tttatctccc tgtggtcatt taaggtcttt ttgtggttct tggcgtccag
     1801 acctgtaaaa agtgaagtat gcgatggcat tgtgttgctt ggatatacag tttgggcgtt
     1861 tagagaatat gagctatttt ttagaaatgc ggctaaattt ggtgtggttt gctcagtaac
     1921 agcatccggt cttagtccgt ctatggtgat gagaaataga tattttccat tagaatcagc
     1981 attggatatg cacgaagtaa taaaaaataa tattaataga taaggtagta ttattcgcaa
     2041 agtaatgaat agattagccg acttatatta aaatggtatt tcgcgtaatt ataaaaatat
     2101 caatgctatt ttgccatgcc tccgccttct actggtgaag tatcaccaaa ggtggaaata
     2161 agagtattaa taaattcttc agagtgagcc tcacagaaaa agtaggttag atatctcatg
     2221 ttattattgg aaactgatag tatctgatag tgtctgctgc aaaggtccgt tccgcaaagt
     2281 gcacattctt taatctgcga tctttctcct tccatatcgc atctctgtcc atccttatct
     2341 ttataatcac agtgaaatag atctattttg acagttcttt tcgacattct tcactccttg
     2401 aaaaaattgc taaagttcta aaattttctg ttaaactaaa tacagttatt ttaataaccc
     2461 attaaactaa atttatatac tttagtcaag aacaatatat atggcttaga gtaattatat
     2521 taaaggagat tcatttttta cttgagtatt taggtaaatt atgtttatat ttcgcccatg
     2581 ataccaatga aagattgtgt gcctggtgta gcaaagtttt tgtacatgaa ggctgcgaga
     2641 aggccaaagc tagtaaacct tgaaattacc aagctctgta atgcagggtg tgacttttgt
     2701 gattactggc agacaaagca cgaggaaagg cttacagatt acggccctgt gatagagaaa
     2761 ataaatccac ttgcagtagt tattacgggt ggtgaaccta tgctgcgaaa ggatcttgtg
     2821 gatatagtaa aacaggtaaa aggttctacc ttttatgtat ttacctctat ggttacaaag
     2881 ggtgacctgc ttacggtgga aaaagcgcat gaccttttta atgccgggct tgatcagatc
     2941 gccgtatcac ttgattttat tggaaagact cacgacaagt acagaggcat agatggcctt
     3001 tgggaatacc ttgaagatct tcttccaaaa cttggtaaag aatttaaagg caagaagagt
     3061 gtcattatta acaccataat tatggatgat aatctggatc agatagttga tctcgtacat
     3121 caggcaaaac agtggaatat tggagtatcc ttcagctctt actctgtaat gaagactaat
     3181 aacgagtctc actatattga taccaatctg caaaaggtta cagaagtggt tgatgagctt
     3241 ataagactta gaaaagagct taaaggcact attcttacta cagagtacta tctaagagag
     3301 ataccgggtt attttaaaag gggaggggtg cctgactgca cagctggtct tagtcatata
     3361 caggttacac cgagcggtca tttaaagagg tgttcagaga tgccagtatc tgctcattat
     3421 tctgagtaca gtcctgatct ctatcaaaaa accaattgcg atgcatgctg gtttagctgt
     3481 aggggtgaga ctcaaagccc tgcaaacgta caacgagccc tcgaatacct tggggtctgg
     3541 gtttaactct taactctcca gattgttcct tcagaagagt cttcaatcac gatacccttt
     3601 gagtttaagt agtctctgat ttcatcactc ctctgccagt tcttttcagc ccttgcgctg
     3661 tttctttgct ctatcagatc gtttatctct tcttcagaaa tatcagactc cgatttagag
     3721 agtttttcct tttgcagata ttcatcgggg tcctgctcga gtattccaaa ggtagttccc
     3781 aataacctta tctctgaaac agcatcatcg agtgttggtg atccaccttc tgaatctata
     3841 gatttattta tcaccctgca aaaatcaaac aggtttccaa gtgcaccagc agtattaaaa
     3901 tcgtcctgca ttgcactttc ccatgactct ctaaactttt ctacatgttt ttttaactct
     3961 tcatcctcat taaccttaat ggttttaatg ttcttaattc tctgtagtgt tagatacaat
     4021 ctttgaagtg acgcctcggc gtcgtctagg ttttgctgcg aaaaatcggc agggtttctg
     4081 taatgatgtg ataaaaagaa aagtcttatg gcctctttgc tccatctctt aagcgcttct
     4141 cttacgttta tgatattgcc gatggatttg gacatctttt ccttatcaat ctgaatgagt
     4201 ccgttatgaa tccagtactt tgcaaactgc tcatctgtag cggcttctga ctgtgctatc
     4261 tcattttcat gatgaggaaa tataaggtcc ttgcctcctc catgaatctc aaagtcctgt
     4321 cccaggtatt tcatgctcat ggtagagcat tcaatgtgcc agccgggtct gccgtttccc
     4381 cacgggcttt cccaaaaagg ctcgccttcc tttgcaccct tccaaagagc aaaatcaagt
     4441 gggttttcct tgttttcgtt tacatcaatc ctgctgccag caatcatgtc atcaggcgtg
     4501 cgcctcgata gttttccata gcctataaac ttatctattg aaaagaagac atcatttcca
     4561 ctaacataag ctacgtcttt attaatcagt gtctctatta gattgataat gtcatggata
     4621 tggtcggata cctttggttc gtggtcaggt ggaagaattc ccagagcggc catatcctct
     4681 tgatattcct ttgtatatct ctctgatact tcagttgctg ctataccaag actatttgcc
     4741 ctttcaatta ttttgtcatc aatgtcggtt acgtttctta cgtagttaac gtcatattgt
     4801 aggtgtttta gaaaccttct gatgatatca aaagtcagtg ctgcgcgggc gtggccaagg
     4861 tgtgcggaat catacaccgt tgggccgcac acatacatcc gtataatgtt ttcatctttt
     4921 ggtacaaggg tttctttctt
//
LOCUS       DBNGSV010000324         5038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1166752, whole genome shotgun sequence.
ACCESSION   DBNGSV010000324 DBNGSV010000000
VERSION     DBNGSV010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1166752"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC159U_11515"
     CDS             complement(<1..371)
                     /locus_tag="AC159U_11515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3337721.1"
                     /translation="MEFKDKVVIVTGAGTGIGKEIATEFAKLGAKIAIAGRRMSKLGE
                     VKKIIEKNSGTCLTIQADVSKVSDTKNIVTQTKKVFGGIDILVNNAGVYIPNPISNVT
                     EKEYDKVMDINMKGTYFLTKHA"
     gene            535..2385
                     /locus_tag="AC159U_11520"
     CDS             535..2385
                     /locus_tag="AC159U_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3337722.1"
                     /translation="MDSSEGLLWMTVISIAAGIISQIGAAFLGIPSIVPLLIMGIIIG
                     PEVLGFLDPHMFGGGFEAIIKLCVAIILFEAGLNLDKDEIKRDQKVIFRLLSAGSLIT
                     LFLTAIFTKLILGLTWSLSLLFGSLVIVTGPTVIQPLLRRVKVGSKLRNILESEGIFI
                     DPIGAIIAIFVLEIVLEESSSLIYSFMLVFVRLGIGAIIGIVGGYIIGRFILRWSIEL
                     EEIIELMVLAAALSVYAISESVLHESGLMAAVACGAVLGHMEIPEEEPLKKFKGKLSI
                     FVISFLFILLAASLKFEYITSLGIGGLLVVFSIIFVVRPVQIFLVTWGSSLNLREKAF
                     LSYISPRGIVAASITSIFAIELNKRGLVGGDIIQGLVFLTIGISVLLQGVTANKIAGL
                     LNLIVEKKKVIIVGANSFGRMIGKILKQHGKEVGFIDTNESHAKIAEMEGFEVIEGSS
                     LDVDNLEKIGIAETGTLLSLTTSNKVNTLVCRLAKVDYGLKNVFPLLNQFKAIATKET
                     VAKLGLDAAFCKPLNLEDLISVTANLRYILNKVEIDESISGKKVEELDLPDNIIPIIL
                     LKGKDKVPHLCRNNLIINKGDELVVYVYGSMDYDLAEFGFFNKEVMKLYF"
     gene            2406..2627
                     /gene="xseB"
                     /locus_tag="AC159U_11525"
     CDS             2406..2627
                     /gene="xseB"
                     /locus_tag="AC159U_11525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3337723.1"
                     /translation="MKKFEKSIEELKDIIDKLESGNIPLEDSLSLFEKGVKILNSANK
                     KLSQMEKKVEILIKDSDGKIKREDFDTSE"
     gene            2633..3529
                     /locus_tag="AC159U_11530"
     CDS             2633..3529
                     /locus_tag="AC159U_11530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3337724.1"
                     /translation="MSFDLTSYLTSKKELIDKELLAFVNGLNADFATLKESILYSINA
                     GGKRLRPIICLASCEALVQDSSKAVPVACALEMIHTYSLIHDDLPCMDDDSFRRGKPT
                     NHKVFGESTAVLAGDALLTDAFHLLSKSIIESDVSADTGLKIIEDISRAAGSEGMIGG
                     QALDLYLEGKEDISLEIVEKMHGLKTGALLGCSVIVGARIGGADEEQLESFSKIGNCM
                     GLAYQIIDDVLDIEGGDDLGKDTGVDIKKNKVTYPLVIGIENAREKVEQLLSTALSEL
                     DKFGSSAQPLISITKYLGSRKN"
     gene            3510..4265
                     /locus_tag="AC159U_11535"
     CDS             3510..4265
                     /locus_tag="AC159U_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3337725.1"
                     /translation="MAAEKTKKKDRIDVVLHERGFVESRNAAKSLLMSGSVFVDGQKI
                     DKAGKIISLDSEITIKDARPKYAGRGGLKLEKALIEFHVDVRDKVCLDIGSSTGGFTD
                     CLLQHGALRVHAIDVGYGQLDWKLRHDPRVILKERLNARYLKQEDIGEPVDLITIDVS
                     FISITKVIEPALSILKSGGALIGLIKPQFEVGKGEVGKGGIVRDKEKHRAVVNKVENY
                     LKDMGLQDIKTIDSPILGAEGNKEFLVYATKPE"
     gene            complement(4272..4922)
                     /gene="fsa"
                     /locus_tag="AC159U_11540"
     CDS             complement(4272..4922)
                     /gene="fsa"
                     /locus_tag="AC159U_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR3337726.1"
                     /translation="MEFFLDTANLDEIRDCASYGILDGVTTNPSLVSKEGEQKGFKEL
                     VKEICEIVDGPVSAEVLSTDVERMISEGKELAEVDDRIVVKMPLTEDGIKATKILSDD
                     GVNVNVTLCFSPMQALIAAKAGAAYISPFVGRLDDISESGMQLIRDIVKIYDNYDFKT
                     KVLVASVRHPDHMVESAKAGAHVATLPYKVFKQLFKHPLTDIGLERFLADWGNVKS"
BASE COUNT         1520 a          893 c         1085 g         1540 t
ORIGIN      
        1 gcatgttttg taaggaaata tgtgcctttc atatttatat ccataacctt gtcatattct
       61 ttttcagtta cattactaat agggtttgga atatagactc cagcgttatt aaccagtata
      121 tcaatcccgc caaacacttt tttagtttga gtaactatat tcttggtatc gctaaccttg
      181 gacacatctg cctgaatggt taggcaagtg cccgaattct tttcaataat ctttttaacc
      241 tcaccgagct tgctcattct tcttccggct attgctattt ttgcgcccaa ttttgcaaat
      301 tcggtagcaa tttccttacc aattcctgtt cctgctcctg tcacgatcac gactttatcc
      361 ttgaattcca ttatgactcc ttttttcagt ttttagattg attgagtaat tatctaaaat
      421 tttttgttta cagcactata atatcacatt aaaattacat ttgggatttt ttgaatctgc
      481 ctcggttatc aatctttgta ggtctttaaa caattatact attggagtgt tgatttggat
      541 agtagtgaag gtttattatg gatgacagta atctcgattg cggctggaat tatatcgcaa
      601 atcggagccg cttttttggg aataccaagc atcgttccct tgctaatcat gggcatcatt
      661 ataggccccg aggttctggg ctttttagat cctcatatgt ttggtggtgg ttttgaagca
      721 ataataaagc tgtgcgttgc cattattctg tttgaagcag gccttaacct tgataaagac
      781 gaaattaaaa gagaccaaaa agtaatattt cgcctgttga gcgccggaag tctcatcaca
      841 ctttttttaa ccgcaatatt tactaaatta atattaggac ttacctggtc attatcactt
      901 ctttttggct cccttgtaat tgttacaggc cctacggtca tacaacccct gttaagaaga
      961 gttaaggtcg gttcaaaact aaggaatatt cttgagtctg aggggatttt tatagatccc
     1021 attggtgcta ttattgcaat ttttgtcctg gagattgttc ttgaggaatc ttcttcactg
     1081 atttacagct ttatgcttgt gttcgtaaga cttggaattg gagcgattat cggaattgtt
     1141 ggtggttaca taataggaag gttcatactg cgctggtcga ttgagcttga agaaataatt
     1201 gaacttatgg tactggcagc ggcgctttct gtttatgcaa tctctgaatc tgtgctacac
     1261 gaatcgggtc ttatggcagc cgtagcctgt ggtgcagtgc tcgggcacat ggaaattcca
     1321 gaagaggaac cgcttaagaa atttaagggt aagctgtcaa tttttgtaat atcatttctt
     1381 tttatactgc ttgctgcaag tctcaaattt gagtacataa cgtctcttgg tattggcggg
     1441 cttcttgtag tattttcaat catatttgtt gtaagaccgg tacagatatt tttggtaaca
     1501 tggggttcga gccttaatct tagagaaaaa gcatttttat cttacatatc tccccgaggg
     1561 attgttgcag catctataac atcgattttt gctatagagc ttaataagag ggggcttgtt
     1621 ggcggtgaca ttattcaggg ccttgtgttt ctaactatag gtatatcggt acttctacag
     1681 ggcgttactg caaacaagat tgcaggactt cttaatctca ttgttgaaaa aaagaaagtc
     1741 attattgttg gagccaattc atttggaaga atgattggga aaattttaaa acagcatggc
     1801 aaggaagttg gctttattga tactaacgaa agccatgcca agattgctga gatggaagga
     1861 tttgaagtca tcgaaggaag cagcctggat gttgataact tggaaaaaat tggaattgct
     1921 gaaacaggca cacttctttc gctcacaaca agtaataaag taaacacact tgtgtgcaga
     1981 cttgccaagg ttgattatgg gcttaaaaat gtgttcccac tactaaatca gtttaaggct
     2041 attgcgacta aggaaactgt tgcaaagctt ggacttgatg cggcattttg caaacctctg
     2101 aatttagaag accttatatc ggttacggca aatttaagat atatactgaa caaggttgaa
     2161 attgatgaat caatctcagg gaaaaaagtt gaagagcttg atcttcccga taacattatc
     2221 ccgattatat tgctgaaagg aaaggataag gttcctcacc tgtgcagaaa taatctaatt
     2281 ataaataaag gtgatgagct tgtggtatat gtctacggaa gcatggatta tgaccttgca
     2341 gaattcgggt tttttaataa agaggttatg aaactatatt tttaggtata aacaggacaa
     2401 aaatagtgaa aaaatttgaa aaatcgattg aagagctcaa agatataatt gataaactcg
     2461 aatccggaaa catcccgctt gaagactcct tgtcgctttt tgaaaaagga gtaaagattc
     2521 taaattcggc caataagaaa ctttcccaaa tggaaaagaa agttgagatt ttaatcaaag
     2581 actcagacgg caaaatcaaa agagaagatt ttgatacgag cgaataatca caatgtcatt
     2641 tgatctcact tcttatctca cttcaaaaaa agaattaatc gataaggaac tgcttgcatt
     2701 tgtaaacggt ctaaacgcag actttgcaac actcaaagaa tccatcttgt atagcataaa
     2761 tgccggaggc aagaggctaa gacccataat ctgtctggct tcctgtgaag cattggttca
     2821 agattcttcg aaagccgtcc cggtcgcatg tgcgcttgaa atgattcaca catactctct
     2881 catacatgat gaccttccgt gcatggatga tgattccttt aggagaggaa aacccacaaa
     2941 ccacaaggtg tttggagagt ccaccgcggt actcgcaggt gatgcacttc ttactgatgc
     3001 ctttcatttg ctgtcaaaaa gtattattga atcagatgta tctgcagata caggacttaa
     3061 aatcattgag gatattagca gagctgcagg ctcggagggg atgatcggcg ggcaggcgct
     3121 ggatttatat ctggagggaa aagaggatat atctttagaa atagttgaga agatgcatgg
     3181 actaaaaacc ggggcgttac taggatgctc agtaatagta ggagcaagga taggcggggc
     3241 tgacgaagaa caattggagt cttttagtaa aataggaaac tgtatggggc ttgcatatca
     3301 aataatagat gatgtactgg acatagaagg tggggatgat ctgggtaaag acacaggtgt
     3361 tgatattaaa aaaaataaag tgacctatcc tttggtgata ggtatagaaa atgcaaggga
     3421 aaaggtcgag caattactga gcacagcact atctgagctt gataaatttg gaagctccgc
     3481 tcagccgctt atatcaatca caaaatatct tggcagcaga aaaaactaag aaaaaagata
     3541 gaatagatgt tgttcttcat gaaaggggtt ttgtagaaag cagaaatgct gcaaagtcgc
     3601 ttctaatgtc cggctctgta tttgtagacg gccaaaagat agataaagca ggaaaaatta
     3661 tcagccttga ttcggaaatt accataaaag atgcccgccc aaaatatgca ggcaggggag
     3721 ggctaaagct tgaaaaagct ctaatcgaat ttcatgttga tgttagagat aaagtttgtc
     3781 tcgatattgg ttcatccaca ggtggattta ccgactgcct attgcaacac ggagctttga
     3841 gggttcatgc tatagatgta ggctacggac agcttgactg gaaactaaga catgatccaa
     3901 gggtaatatt aaaagagcgg ttaaatgcca ggtatctaaa acaagaagat attggtgaac
     3961 ctgtggattt aattacaata gacgtatctt tcatctccat aaccaaggtc atagaacctg
     4021 ctctctcgat attaaaaagt gggggggcgt tgattggtct catcaaacct cagtttgaag
     4081 tgggcaaagg tgaagtggga aagggtggca tagtccgcga taaggaaaaa caccgtgccg
     4141 tagtaaataa agtcgagaat tatttaaaag atatgggact gcaggatata aaaactattg
     4201 attcaccaat cctgggagca gaggggaata aagagttttt ggtttacgca accaagcctg
     4261 aataatatgc atcagctttt aacatttccc caatcggcta agaacctttc aagcccaata
     4321 tctgtgagcg ggtgtttaaa aagctgtttg aaaaccttgt agggcagtgt agccacatga
     4381 gctcctgctt ttgcagattc taccatatga tccgggtgcc ttacgcttgc cacaagaacc
     4441 ttggttttga aatcgtagtt atcataaatc tttacaatgt cccttataag ctgcattcct
     4501 gattctgaaa tatcatccag ccttcctaca aatgggctta tgtatgctgc acctgccttg
     4561 gctgcaatta gagcctgcat tggagagaag cagagggtaa catttacatt tactccgtca
     4621 tcagaaagaa tttttgtagc ttttatcccg tcttcagtaa ggggcatttt tacaactatc
     4681 ctgtcgtcaa cctcggctag ttcttttccc tcggagatca ttctttctac atcggtgctg
     4741 agcacttcgg cgctcactgg gccgtccact atctcgcata tttcttttac aagttctttg
     4801 aacccctttt gttctccttc tttagagaca agtgaaggat tagtagtaac cccgtccaga
     4861 atgccgtaac ttgcacagtc tcttatttcg tctaggtttg cagtgtctaa gaaaaattcc
     4921 atttatgacc tccggggtgt ttaagaatta taaaaaaata aaatatatat aatccctgag
     4981 attttacaaa atagtaaggt agtaataagt ttgggcatta gacatctggc ggagtaga
//
LOCUS       DBNGSV010000325         2429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_682976, whole genome shotgun sequence.
ACCESSION   DBNGSV010000325 DBNGSV010000000
VERSION     DBNGSV010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_682976"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1039)
                     /locus_tag="AC159U_11545"
     CDS             complement(194..1039)
                     /locus_tag="AC159U_11545"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3337727.1"
                     /translation="MSYPNSDILVSPAWLSEHLDDPNIVIVDCPWAADAYTKAHIPGA
                     VCRPGHCYLKSTDSHGNMSIFLQNESEFEKLCGELGIGADSTIVAYDEWGSIFATRLW
                     WMLKFYGHTNAKVLDGGWQGWVDAGYPVSYKKPEVSASSDFKVTAYPELLIELDELKQ
                     NYSNDNWQVLDVRSEDEYNGKAAHGNKRVGHVPGAIHLEWNRFLNNSTDSEAVRNFKS
                     AEQIQALLDGSGVDKNKIIATHCQAAVRATFGAFVLELMGYPPAKVYDGSMAEWANLD
                     DTPLE"
     gene            complement(1113..1898)
                     /locus_tag="AC159U_11550"
     CDS             complement(1113..1898)
                     /locus_tag="AC159U_11550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3337728.1"
                     /translation="MVFPKLAVSLMLVTGALAEIKIQELSYKADDTTLKGFLAYDDSI
                     KEKKPGVIVVHEWWGHNDYARARATKLAELGYVALAVDMYGDGKTADHPDNAKKFMTE
                     ALANQELFLKKFQAGLELLKSQPQTDAEKVAAIGYCFGGATVLGMARAGLDLDGVVSF
                     HGILATETPAQKGAVKAKVLAFHGDNDPFVPKEQADAFRKEMNDAEVDYTFVVYPGVQ
                     HSFTNPGATEVGTKYELPLVYDAEADASSWKQTQEFFKEIFAK"
     gene            1946..>2429
                     /locus_tag="AC159U_11555"
     CDS             1946..>2429
                     /locus_tag="AC159U_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3337729.1"
                     /translation="MTETSNKKSLTTLLLKYKYSVIAGLIALTVVDLCQLSVPIIIQR
                     VVDTLTEENSTYSDLNKYAVYILILAALMSLFRFGWRYFLMGASRKLEQSLRNEFFSH
                     LQSLDFDFFNKRKTGDLMAHTVNDIETIRMACGLGMIIAYDGIFLLIFIMIAMLYISP
                     E"
BASE COUNT          670 a          544 c          455 g          760 t
ORIGIN      
        1 aaagatacga aagaaaatat gataagaaat ccttttaata tgttttgtct gtccatcgaa
       61 ctttttgcct gtgttattta taaatttaga ctcagacagg ccgtgttaag attcttaata
      121 tttatacgaa tataagagct aagaagtttc aaccattaca aattatcgca tgcaaatgat
      181 cttttaggag atactactca agcggagtat catcgaggtt tgcccattct gccatcgagc
      241 cgtcatatac ctttgctgga ggatagccca taagctccag tacaaatgcg ccgaaagtgg
      301 cacgcacggc agcctggcag tgagttgcaa ttattttgtt tttatctacc cctgaaccat
      361 ctaaaagtgc ctgaatttgc tcggctgatt taaagtttct cacagcttca gaatcagtcg
      421 agttattcaa aaacctgttc cattcgagat gaattgcgcc cggcacatgc cctactcgtt
      481 tatttccatg tgcggcttta ccgttgtatt catcttcaga gcgcacgtca agaacctgcc
      541 aattatcatt cgaataattt tgcttaagct catcaagctc aataagtagc tcgggatatg
      601 ctgtcacttt aaaatccgag ctggccgaaa cttccggctt tttatatgaa acaggatagc
      661 cagcatctac ccagccctgc catccgccgt ctaaaacttt ggcatttgta tgtccataga
      721 atttaagcat ccaccagagc cttgttgcaa aaatagagcc ccattcgtca taggcaacaa
      781 ttgtagaatc tgctccaata ccaagctctc cacacagttt ttcaaactct gattcatttt
      841 ggagaaatat actcatattt ccgtgagaat ctgtagattt gagatagcag tgcccgggtc
      901 tgcatactgc accggggata tgggctttgg tataggcatc cgctgcccaa gggcagtcta
      961 caattacgat attaggatcg tcaagatgtt cagaaagcca cgcggggctt acaagtatat
     1021 cagagtttgg ataagacatt tttatctccc ccttgccctt taaatcagac tggctgcaat
     1081 aaacttacaa ccagtctcta ttaaactaat ttttacttag caaaaatctc tttaaaaaat
     1141 tcctgagtct gtttccatga acttgcatca gcctctgcat cataaacaag cggaagctca
     1201 tattttgtac caacttcagt tgctcctgga ttggtaaaac tgtgctgtac tcccggatat
     1261 actacaaatg tataatcaac ttcagcatcg ttcatctctt ttctgaatgc atcagcctgc
     1321 tcttttggaa caaacgggtc attatcgcca tgaaaagcca gaacttttgc cttaacagct
     1381 cctttttgtg ccggagtctc agttgctaag attccgtgaa agctaactac tccgtcaaga
     1441 tcaagccctg ctcttgccat accaagcaca gtagctccgc caaagcagta tcctattgca
     1501 gctacttttt cagcatctgt ctgcggctgg ctctttagaa gttcaagccc tgcctgaaat
     1561 ttctttaaaa acaattcctg atttgctagc gcttcggtca taaacttctt tgcattgtcg
     1621 gggtgatctg cggtttttcc gtcgccatac atatcaactg caagcgctac atacccaagt
     1681 tcggcaagct tggttgctct tgctcttgcg taatcattat gtccccacca ctcgtggact
     1741 acgattactc cgggtttttt ctcttttata gaatcgtcat aagccagaaa accttttagt
     1801 gtggtatcgt cggctttata actaagctcc tgaatcttta tctctgccag ggcgcctgta
     1861 actaacataa gtgatacagc aagtttcgga aaaaccactg aatagactgg tatacttaaa
     1921 tgcttataaa ttttatcctg gcaaaatgac tgaaacaagc aacaaaaaat ctcttacaac
     1981 tctgctttta aaatacaaat actctgtaat tgcaggcctt atagcactta ctgtggtaga
     2041 tctgtgtcag ttatcagtgc ctattattat tcagagagtg gttgatacgt taactgagga
     2101 gaattctacc tattctgatc ttaataaata tgctgtttat attttgatcc tggctgccct
     2161 catgtcactg tttcgttttg ggtggaggta ttttcttatg ggcgcatcga gaaagttaga
     2221 gcagtcgtta agaaatgagt tcttttccca ccttcaaagc cttgattttg atttttttaa
     2281 taaaagaaag acaggtgacc tcatggctca taccgtaaat gatattgaga ccatcagaat
     2341 ggcttgcggg cttgggatga taatagccta tgacggcata ttccttctta tattcataat
     2401 gattgcgatg ctctatattt cacctgagc
//
LOCUS       DBNGSV010000326        14578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_960003, whole genome shotgun sequence.
ACCESSION   DBNGSV010000326 DBNGSV010000000
VERSION     DBNGSV010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14578
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_960003"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC159U_11560"
     CDS             complement(<1..365)
                     /locus_tag="AC159U_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337730.1"
                     /translation="MPNTSILREIELLINSNHSIIFIETAEEERADALIKHIADAMNI
                     GFFIWSASKGLRRVDGEEVKGSVYQTTELSLALGHVESSGFAAVYHFQGLSDYFEDRI
                     LVTRLKDTAKTFTDKKGAI"
     gene            511..1497
                     /locus_tag="AC159U_11565"
     CDS             511..1497
                     /locus_tag="AC159U_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3337731.1"
                     /translation="MKAMVLERSRAALELRELDIQKPNPEQVLLKVKACAVCRTDLHI
                     VDGELAEPKLPLILGHEIVGEIIELGSNVGGFSTGDIIGVPWLGQTCNKCKFCESGKE
                     NLCDNAKFTGYQIDGGYAEYAVANHNYCFTIPKDYSAINAAPLLCAGLIGYRSYRMTG
                     SSKKIGLYGFGAAAHIIAQVAVFESKELYAFTKSGDTQAQKFALELGCEWAGDSNNPP
                     PDKLDSAIIFAPVGSLIPAALRNVVKGGTVICAGIHMSDIPSFPYDILWGEREIKSVA
                     NLTRKDGEEFFAIAPRVPVKTEVITYELEQANEALNDLRTGKINGAAVLVME"
     gene            1497..2186
                     /gene="aat"
                     /locus_tag="AC159U_11570"
     CDS             1497..2186
                     /gene="aat"
                     /locus_tag="AC159U_11570"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR3337732.1"
                     /translation="MRQNKIFLLSDDLIFPNPRLADSHGLLAVGGDLSTDRLLLAYEN
                     GIFPWYSEDQPILWFSPDPRLVLFPDKFIVSKSLQKTIKKNIFEVKFDTNFEEVITEC
                     SKIERKDQDGTWITQDMIDAYIELHNQGYAHCVETYQEGNLVGGLYGVSLGGAFFGES
                     MFHKMKDASKIALHYLVQKVKKWEFDFIDSQVVTEHMKSMGSQEITRNKYLELLGKTL
                     KKETRSGTWET"
     gene            complement(2386..3711)
                     /locus_tag="AC159U_11575"
     CDS             complement(2386..3711)
                     /locus_tag="AC159U_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR3337733.1"
                     /translation="MIEKIFKLGENNTNIKQEIVAGFTTFATLSYIIFVQPAVLSAAG
                     MDYGSALVATCLASAIGMLLMAFLTNYPIALAPAMGHNFYFTYVVCLTMGVIWQQALG
                     AVFIAGIIFIALSLTGLREKVMDIFPDALRNGIPVGIGLLIALIGLEWSGIVTSHPVT
                     YVTLGDLHSKYTLISIFGLIVIAFLLAIKFKGAILTGIIGCSVLALILGVVEFNGVVS
                     SPPSIEPSLLKLQIPDLFANLEFITVIIVFLFLDLFDTIGTLVGVGQLGGFMKNGKLP
                     KAKQALLSDAVATSTGALLGTSTVTSYIESSSGISAGGRTGLTNVVTAILMIAAIFFT
                     PLIQMVGAGIDVGGGKFLYPVIAPALIIVGSMMMTNVKIINWDDYTESIPAFLTIIIM
                     PLSFSITEGIAFGFISYSLLKIFSGKAKEVNWIIYLISFLFIARYIWLV"
     gene            3870..5249
                     /locus_tag="AC159U_11580"
     CDS             3870..5249
                     /locus_tag="AC159U_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HIR3337734.1"
                     /translation="MAKNYDSIIIGTGQAGPSLAERLSQTGRNIAVIERNLFGGTCVN
                     TGCIPTKTLVASAKVANMVSRAAEYGVNINGDINIDMKRVKARKDEVSGASNKGVEGW
                     LKNMKNVDIYEGHGRFVDNYTVQVNDEILEADNIFINVGGRANVPPIEGLNEVKFFTN
                     SDMMQVDFLPEHLIIIGGSYIGLEFAQMYRRFGSEVTVVEMSDRLISREDEEISENIK
                     EILENEGINFRLSAECITAGKNGKDIQVGVDCNKGDKEISGSHLLVATGRLPNTHDLG
                     IENTDIKLDKRGYIEVDDKLQTNVPGIWAIGDCNGRGAFTHTSYNDFEIVATNILDND
                     PRKVTDRILCYGLFIDPPLGRVGMTEDQARKSGRKLLVGKRNMSRVGRARERSETQGF
                     MKVIVDADTKEILGAAILGIEGDEAIHCILDVMYANAPYTTIQRAVHIHPTVSELIPT
                     VLGSLEPVN"
     gene            5268..6965
                     /locus_tag="AC159U_11585"
     CDS             5268..6965
                     /locus_tag="AC159U_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337735.1"
                     /translation="MKIKSYNLEKYHKAIDAQVSKFEEQSIIQNLFAHDHKIWSESPD
                     EINNRLGWLDSPAEMLESVDEIDSFVTECKEAGLKKVLLMGMGGSSLAPELFNEIFNR
                     NRKGLELRIADSTHPDKVAELRNYFDPKDTLYIVSTKSGGTVETISLMNFFYNQCCNV
                     LGTENTGSHFAAITDPGSKLEEMSDELGFRKCFLNDPNIGGRFSVLSYFGLVPAALVG
                     IDIKQLLLNILEFQNMLDTNTLKLDDPNNPVYLGTIAGTLAEQGLDKLTFIISEELIN
                     FGDWLEQLIAESSGKEGKGILPVIEKEIEDINSYSVDRLYVCISLNNDCSSTSKKIQD
                     AGLPLIGFDLKDKMELGTEFYRWEIATAIACAVLKVNPFDQPNVESTKIISRQFLSEY
                     KEKGEITEPEYIEVEDALRIVGSDISANLSDTISGLFSGNRNNKYISIQAYISQNTET
                     ISRLEEIKTALYKETGIPVTIGFGPRFLHSTGQLHKGDSGNGIFLQIVSEPKEDIEIP
                     DRAGNNKSSLSFGTLMKAQYLGDRSALLDAGRKVLTINIGTNITSGLNNILDCIDTK"
     gene            6965..7651
                     /gene="rpiA"
                     /locus_tag="AC159U_11590"
     CDS             6965..7651
                     /gene="rpiA"
                     /locus_tag="AC159U_11590"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR3337736.1"
                     /translation="MDVNELRNKAAIEAVKNVESGMVLGLGTGATANLALQELSRLIN
                     EGSLKEIVGIPSSNQTEKLALELGIPLITFEQKSEIDLNIDGADEVDLDLNLIKGGGG
                     ALLREKVIAQASNKNIIVCDQNKLSPKLGTKWALPVQIMEFAYRPVAKFVEDLGADVV
                     LRKSQSGDIFKTEQGNIILDCNFGQIDNLSNLNQALNNKAGIVEHGLFLGIADEVIVG
                     KSDSVETLTN"
     gene            7662..8972
                     /locus_tag="AC159U_11595"
     CDS             7662..8972
                     /locus_tag="AC159U_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR3337737.1"
                     /translation="MTIYNLISFGGLFVLLGFAYLLSSDKKNVNYRVIIWGVLIQLIF
                     GIFIFLIPAGTKLFLYLNDIVVQIMNSSAEGAKFLFGRLALPPGTEDEHGQSSLGFYL
                     AFQALPTIIFFSALMSIFYYYGIMQKLIKGFSYLFTKLMRISGAESLSAASNIFVGVE
                     SAFAIRPFLNQMTRSELCTVLTAGMATVSSNILALYIFILQSEFPTIASHLISASILS
                     APAAIVMSKILLPESGKPVTLGLQVNPSYEKESSIFESIINGANGGVKLIVGIAALLV
                     AVLGLVALLNLFLSMIGTPMNSLFGTEFTLSLQNIFGYIFYPFTLIIGIPLTDAFNVS
                     KLIGEKVILTEVVSYQDLAVAIKDQVITNPRSIVVTTYALCGFTHLASLSIFVGGVSA
                     IAPDRKLDIASVGFRALIAATLACLMTACVSGVFFTGSSILLQN"
     gene            9063..9524
                     /locus_tag="AC159U_11600"
     CDS             9063..9524
                     /locus_tag="AC159U_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3337738.1"
                     /translation="MKGNVNVNELSRYEIAECAQCTGFNLKKATRIIQNRFDQAFKPV
                     GLEGTQYTVLAHIFVHGPISLSKVAELMHVDRTTLARNLAPLERKDFIKIKPGIDRRS
                     KIISITDKGMEVLAKALPLWKKTQAEIKETLGIENWSSMLSNLENLVTEMK"
     gene            9578..10687
                     /locus_tag="AC159U_11605"
     CDS             9578..10687
                     /locus_tag="AC159U_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HIR3337739.1"
                     /translation="MSKPRILVTSAAGHTGSVVVKQLLEKGFPVRAFVRRHDARSEKL
                     RRAGAEIFAGDLFDMRDIRSALVSVQLAYYCPPFAPNLLHGSMLFPLAAEEAKLEVVA
                     LMSGWNPHPSHPSVITREHWVTNNIFQWMPSVDVVYVNPGLFAFTYFFGLPAVANFGR
                     LMLPYGEGLNAPPSNEDIASVVSSILQNPAPHIGKNYRPTGPKLISGHDVADILGQIL
                     DRKVTYQDVPTWMFTKAAKAQGFSNFEIAQIRHYAEEVRGGTFAVGAPTDHVEVVTGR
                     PAEDFETTARRYIQHPELVIPNFKIGGKIGAAWLMMKTMLTKAPYLDKWESERGYPLL
                     GSPVLAHDSEEWLQSAKNKNLALIENNQSILRAVV"
     gene            10741..11595
                     /locus_tag="AC159U_11610"
     CDS             10741..11595
                     /locus_tag="AC159U_11610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3337740.1"
                     /translation="MSKKRFEDKVVLITGASGGIGKASGTDFAREGAKLALVSRSKEK
                     LDKVAGHIKQYTNEVITIPTDVSNQDEVNSMVSQVIDRFGNIDYLFNNAGSSYVGGIE
                     KNDFAENVKRMFEVDYMDTVYTTKAVLTHMKKKGSGHIMNMSSVVGRKAFPKFGGYSS
                     IMHAITGFSAALRQELRDSGIKVSTIHPALTQTNLLDHVNPEDMPPPFKNMTPMPVEK
                     VSKAVLDGFHYNQTRIVVPFQPKLVLFWDAVSPAIGDYFVRLLSKRTFSRLIGMYRGS
                     LYQHDTAV"
     gene            11644..12327
                     /locus_tag="AC159U_11615"
     CDS             11644..12327
                     /locus_tag="AC159U_11615"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3337741.1"
                     /translation="MDLEILWASGSPYSWSVLLALEVKKLEYKSTLIELLKGEGRTQE
                     FLKMNPRGKVPVLRDEQTYISESLAILSYLDKKYSEPSLFGNTPREAADIMKIISDYN
                     SYIAPDLNHLISSAFWSKPPNQVGLEEAKQALFYELSNLEKIVSESEWLAGENISAAD
                     IIIFPFMQLLNRSEAKLQKEIGYTLMFSHTRFPKMYEWMRRFEALPGYEKTYPPHWEQ
                     DKGLNHYIS"
     gene            complement(12345..12851)
                     /locus_tag="AC159U_11620"
     CDS             complement(12345..12851)
                     /locus_tag="AC159U_11620"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3337742.1"
                     /translation="MFYVHNLVVLIHVLSAIVWVGGMLFIALVVIPALRNFQPQEKRT
                     EVISATATRFRKIGWIAIIILAITGLINVINRGITHEMIASGSLLSTHFGKVLAIKLI
                     LFVIMILLSLSHDFILGPRHLRLLQSSKSNPAKMSDLNKSKKLVSWLARVNVVISVLI
                     VACAVMLS"
     gene            complement(12904..13128)
                     /locus_tag="AC159U_11625"
     CDS             complement(12904..13128)
                     /locus_tag="AC159U_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337743.1"
                     /translation="MKVLTQSGTFDVSINSVSVEQDVVALNAQMGVWYGSIVLSDDDL
                     WYFTKIFLSPRVLVKVFRAAFRSIFKIGKN"
     gene            13510..13770
                     /locus_tag="AC159U_11630"
     CDS             13510..13770
                     /locus_tag="AC159U_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337744.1"
                     /translation="MKKLLTLTAVALSLGLSSASFAESNVFGVDTPLTKQEIRSNVQG
                     GKVQKGLNDLYLYSTDRVEIGYQEVTSESDNSNYIFGVKINV"
     gene            13977..14186
                     /locus_tag="AC159U_11635"
     CDS             13977..14186
                     /locus_tag="AC159U_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3337745.1"
                     /translation="MAEREVGTVKWFNSTKGFGFIEREGGKDVFVHYSALEGAGYRSL
                     EEGQKVEFTVVEGEKGPQAQDVTTA"
     gene            complement(14259..>14578)
                     /locus_tag="AC159U_11640"
     CDS             complement(14259..>14578)
                     /locus_tag="AC159U_11640"
                     /inference="COORDINATES: protein motif:HMM:NF018534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337746.1"
                     /translation="FVSRNTTQFVKKEEKKEKIRIQIPSNNLHIAYDPRIPEKSQLLE
                     FSIRGVEDNDEVLWNINDEKITGIGSKHLWTVKRGDYRLSAQVLRQGKQVAKTKQISF
                     LVK"
BASE COUNT         4820 a         2713 c         2946 g         4099 t
ORIGIN      
        1 attatggccc cttttttatc tgtaaaggtc ttggccgtat cctttagtct tgtaacgagt
       61 atcctgtctt caaaataatc agacaaaccc tgaaaatgat aaacagcggc aaaacctgaa
      121 ctttccacat gcccaagcgc cagggacagt tctgtcgttt gatataccga gccttttact
      181 tcttctccat ctaccctgcg cagccccttt gatgctgacc agataaaaaa ccctatgttc
      241 atcgcatctg caatatgctt aatcagggca tcagccctct cttcctctgc tgtttcaata
      301 aaaattattg aatgattaga gtttataaga agttctattt ctcttaagat gcttgtgttt
      361 ggcataattt cataagtcct gtaaaaaaca gactaaatca tatttgtaga gcacagctat
      421 ttcaagtcta atttaaaacc ttataatatt tatataagta ttaaaataat atttcaaata
      481 tattggatgc ataaaagtta gaatcaaaca atgaaagcaa tggtattaga aaggagcaga
      541 gccgctcttg aattaagaga gttggatatt caaaaaccta atccagagca ggtcttatta
      601 aaagtaaaag cctgcgcagt atgcagaact gacctgcata tagtagatgg tgaattagct
      661 gaaccgaaat tgcctctgat attagggcat gaaattgtgg gagaaataat agaacttgga
      721 agtaatgtag gtggatttag tactggagac atcattggtg tcccctggct aggacagaca
      781 tgtaacaaat gcaaattctg tgaatcagga aaggaaaacc tatgtgacaa tgcaaagttt
      841 acaggttacc agattgacgg aggctacgcg gaatatgctg tggctaacca taactactgt
      901 ttcactattc caaaagatta ctctgcaatt aatgcagcac cactcctatg tgcaggtctc
      961 attggatatc gttcatacag gatgactgga agttctaaga agattggtct ttatggattt
     1021 ggtgcggcag ctcatatcat tgcgcaggtt gcagtttttg agagtaagga gctctatgca
     1081 ttcacaaagt ctggagatac tcaagctcag aaatttgcat tagaactagg atgcgaatgg
     1141 gctggtgatt caaataatcc tccgcccgat aaactagatt ctgcaataat atttgcgcct
     1201 gtaggcagtc tgataccagc cgctttgaga aacgtggtta aaggtggaac tgtaatctgt
     1261 gcaggaattc atatgagtga tataccttct ttcccatatg atattctgtg gggggagaga
     1321 gagattaaat cggtagcaaa tctgacaaga aaagatggtg aagagttttt tgcaatagct
     1381 ccaagagttc ctgtaaaaac agaggtaatt acatacgagt tagaacaagc caatgaagca
     1441 ttgaacgatc ttagaactgg caaaataaac ggtgcagcag tacttgtaat ggaataatga
     1501 gacagaataa aatattttta ttatcagatg atctaatatt cccaaatcca agattagcgg
     1561 attctcatgg actgcttgct gtaggaggtg accttagcac tgataggttg ctgctagcct
     1621 atgaaaacgg gatattccca tggtattctg aggatcaacc gatattatgg ttttcacctg
     1681 atcccagact cgttctattt cctgataaat ttatcgtttc caaaagcctt caaaaaacta
     1741 tcaaaaaaaa tatctttgag gttaagtttg atacaaattt tgaagaggtg ataaccgaat
     1801 gctcaaaaat agaaagaaaa gatcaggacg ggacatggat aactcaagat atgattgatg
     1861 cttatataga acttcacaat cagggttatg cccattgtgt agaaacctat caagagggaa
     1921 atctcgttgg aggtttatac ggagtttctt taggcggagc attctttggc gaatcgatgt
     1981 ttcataaaat gaaagacgct tcaaagatag cacttcacta tctggttcaa aaagttaaga
     2041 agtgggagtt tgacttcata gattcacagg tcgttacaga gcatatgaaa agcatgggct
     2101 ctcaggaaat tacaagaaat aaatatttag agcttttagg taaaacttta aagaaagaaa
     2161 caagatcagg aacctgggaa acttaaaagt tatccatatt cacacttttt agtctcataa
     2221 ttaaacctgc cgcccgaatc gagacatcta tccgcattat aaaattcaga attgataaaa
     2281 agccatattg ataggcataa caaaactgct agactaacaa aaattaaatg aaatattttt
     2341 acatttttta ggttgtatga aactagtttc atcgatttaa ttttgttaaa ccaaccaaat
     2401 atatcttgca ataaaaagaa acgagatcag ataaattatc caattaactt ctttagcctt
     2461 cccagagaat atctttaata aagaataaga aataaatcca aaggcaatgc cctctgtgat
     2521 gctgaaagaa agtggcatga taataatagt taaaaatgcc gggatagatt ctgtatagtc
     2581 atcccagttt atgattttta catttgtcat catcatgctt cctactatga taagagctgg
     2641 cgctattaca ggatagagaa atttacctcc acccacatct atccctgctc cgaccatctg
     2701 aataagtgga gtgaaaaaga ttgctgcgat catgagaata gcagtaacaa catttgtaag
     2761 cccggtcctt ccgccggcgc ttatgcccga tgagctttct atgtagcttg taacagtaga
     2821 agtaccaaga agtgcacctg tgcttgtggc aactgcatct gaaagaagag cctgttttgc
     2881 cttaggaagc ttaccgtttt tcataaaccc gccaagctgc cctaccccaa ccagggttcc
     2941 tatagtgtca aacaaatcca gaaacaggaa aactattatc actgtgataa attccagatt
     3001 tgcaaacaag tccggtatct gaagcttaag caaggaaggc tctatcgatg gtgggcttga
     3061 aaccactccg ttaaactcaa caacccccag gatcagtgcc agcacagaac atccgataat
     3121 tccggttagt atcgcccctt tgaattttat ggccagtaga aatgctatta caatcaggcc
     3181 gaaaattgag attagggtgt attttgaatg caggtctccc aatgttacat atgtcacagg
     3241 atgtgaagtt acgattccag accactccag accgattaaa gcaattagaa gcccaattcc
     3301 aacgggtatt ccgttcctga gagcatccgg gaatatatcc atcacttttt ctctaagacc
     3361 agtgagcgat aatgcaataa atataattcc tgcaataaat acagctccca gagcttgctg
     3421 ccatattact cccattgtaa gacagacaac ataggtaaag taaaagttat gtcccatagc
     3481 aggggcaagg gcaatcggat agttcgtaag aaaagccata agcagcatcc ctattgcact
     3541 agcaagacaa gtggccacaa gtgcagagcc atagtccata cctgccgctg aaagaacggc
     3601 gggctggaca aatataatgt atgaaagtgt cgcaaaagta gtaaagccgg caacaatttc
     3661 ctgcttaata tttgtattat tctctcctaa cttaaaaatc ttttcaatca tatcctgagt
     3721 ccaatatagg gctaaataat atcataatat aagttggaga atcaaacagt atagaatcaa
     3781 attatatatt ctgtattact tagagtttat ttagtataat tgctccaggt gtttatatac
     3841 ccagaatgag gagaagatga ggagagatca tggcaaagaa ttatgattca ataataattg
     3901 gaaccggtca ggcaggccct tcacttgctg aaagactcag tcagacaggc aggaatatag
     3961 ctgtaataga aagaaacctg tttggaggca cctgtgtaaa taccggctgt atccccacaa
     4021 aaacccttgt tgcaagcgcc aaggttgcca atatggttag cagagcagct gaatatggag
     4081 ttaatataaa tggcgacata aatattgata tgaaacgagt taaagccaga aaggatgaag
     4141 tctcgggagc atcaaacaag ggagtagagg gctggctcaa gaacatgaaa aatgtggata
     4201 tatacgaagg acacggaaga tttgtggata attatacagt acaggtaaat gatgaaatat
     4261 tagaagcaga taacattttt attaatgttg gaggcagggc aaatgtacca cctatagaag
     4321 ggcttaatga agttaaattt ttcacaaatt cagatatgat gcaggtagat tttctgccgg
     4381 aacatttgat aataatcggt ggaagctata tcgggcttga gtttgcacaa atgtatagac
     4441 gatttggcag tgaagtaact gttgtcgaga tgtcagacag acttatatca cgagaggatg
     4501 aagaaatatc ggaaaatatt aaagagatcc tggaaaatga aggaataaat tttaggctat
     4561 cagctgaatg tataactgct ggcaagaatg gcaaggatat tcaagtgggt gttgattgca
     4621 ataaaggaga taaagaaatt tctggatcac acttattagt agctacggga aggcttccaa
     4681 atactcacga tcttggaata gaaaacactg atattaagtt ggataaaagg ggttatatag
     4741 aagttgatga taaactgcaa acaaacgtac ccggtatttg ggcaattggt gactgcaacg
     4801 gcaggggagc atttactcac acctcataca acgactttga gatagttgca accaacatcc
     4861 tcgataatga tccaagaaaa gtgacagaca ggattttatg ttacggactt tttatagacc
     4921 ctccgctggg tagagtagga atgacagaag atcaggcaag aaaatccggc aggaaactgc
     4981 ttgtcggcaa aagaaatatg agccgggtag gcagggcaag agaaagaagt gagacccagg
     5041 ggtttatgaa agttattgta gatgctgata caaaagagat tctcggtgcg gcaatcctgg
     5101 gtatagaagg cgatgaagcc atacactgta tattggatgt aatgtatgca aatgcgcctt
     5161 atacgaccat acaaagagct gtacatattc acccaacagt gtcagagctt attccaactg
     5221 tactgggcag tttagaacca gtaaattagt tatacaattg aaatcaaatg aaaataaaat
     5281 cttacaattt agaaaaatat cataaagcaa ttgatgctca ggtaagcaaa tttgaagagc
     5341 agagtatcat tcaaaatctt tttgcccatg accataaaat ctggagcgaa agcccggatg
     5401 aaataaacaa caggcttgga tggctggaca gcccagcaga gatgcttgag agtgtggatg
     5461 aaatagactc atttgttact gagtgtaaag aggccggatt aaaaaaagtg cttcttatgg
     5521 ggatgggcgg ctcaagtctt gcaccggaat tatttaatga aatattcaat agaaaccgaa
     5581 aaggattgga gcttagaatt gccgactcta ctcatccgga taaggttgca gaattaagaa
     5641 actactttga tccgaaagat acactttata tcgtctccac taaatcaggt ggcactgtcg
     5701 agacaatttc actcatgaac tttttctata accagtgctg taatgtttta ggaacagaaa
     5761 atacgggaag ccattttgca gcaataacag accccggcag taagctcgaa gagatgtcgg
     5821 atgaattagg atttagaaaa tgcttcctga atgatcctaa tataggcggc aggttttccg
     5881 tgttgtcata tttcgggctc gtacccgcag cgcttgtggg aattgatata aaacagcttc
     5941 ttcttaatat tctcgaattc cagaacatgc tggatacaaa cactctaaag ttagatgatc
     6001 ccaataatcc agtctatctt ggaacgattg ccggtacgct tgcagaacaa ggattggata
     6061 aacttacttt tataatatct gaagaactaa taaactttgg cgactggtta gagcagttaa
     6121 ttgcagagag cagtgggaaa gagggtaagg gcatactacc agtcatagaa aaagagattg
     6181 aagatattaa ttcatattct gtagacaggc tatatgtttg tattagtctt aataatgatt
     6241 gttcttccac ttccaaaaaa atccaggatg ccggattacc tctaattggc tttgatctta
     6301 aagacaagat ggagcttgga acagaattct acagatggga aattgctacg gctattgctt
     6361 gtgctgtcct aaaagtaaac ccattcgatc agccaaatgt ggagtcaact aaaataattt
     6421 cgagacagtt tctttctgag tataaagaaa aaggtgaaat tacagagcct gaatatattg
     6481 aagtcgagga cgcattgaga atagtgggga gcgatatttc ggctaatctc tcagatacca
     6541 tatctggttt attctccggc aatagaaata ataaatatat atccattcag gcgtatattt
     6601 ctcagaatac agaaacgata agtaggttag aagagatcaa gacagcatta tataaagaga
     6661 caggaattcc cgtaacgata gggtttgggc caaggttctt acactccaca ggacagcttc
     6721 ataagggcga cagcggaaat ggcatttttt tacaaattgt ttcagaaccg aaagaagata
     6781 ttgaaattcc tgacagggct ggcaataata aatcgtcact tagttttgga acactcatga
     6841 aggctcaata ccttggtgac agaagtgctc tattagatgc aggaagaaaa gttcttacaa
     6901 ttaatattgg cacgaatatt acatcaggtt taaacaacat attagattgt attgatacta
     6961 aataatggac gtaaatgaac ttagaaacaa agccgctata gaggctgtaa aaaatgtgga
     7021 atcaggcatg gtgcttggcc ttggaacagg tgcaaccgca aatctagccc tgcaggaact
     7081 ttccagatta ataaacgaag gttctctaaa agagatagtg ggcataccca gttctaacca
     7141 gactgaaaag ctggccttgg agcttggcat accccttatt acttttgagc aaaaaagtga
     7201 aatcgatttg aacatagacg gtgcagatga agttgacctc gatctgaatt taattaaagg
     7261 cggcggtgga gcgctgctta gagaaaaagt catagctcag gcaagcaata aaaacataat
     7321 agtctgtgat cagaataaac tgtctcctaa gcttggtacg aaatgggcac tccctgtaca
     7381 gataatggaa tttgcatata ggcccgttgc caaattcgta gaggacttgg gggcagatgt
     7441 ggttctaaga aaaagtcaga gcggagatat attcaaaacc gaacagggca acataattct
     7501 agactgcaat tttggacaaa ttgataatct aagcaattta aaccaggctc taaacaacaa
     7561 agcaggaatt gtagaacatg ggctatttct tggcatagca gatgaagtaa tcgttggtaa
     7621 atcagactcg gtagagacct taacaaacta gctcttctaa tatgactatc tataacctaa
     7681 tcagttttgg cggactgttt gtgcttcttg gttttgccta tttactctca agtgataaaa
     7741 agaatgttaa ttacagagta atcatctggg gagttttaat ccaactgatt tttggaattt
     7801 ttatattcct tatacccgcc ggaacaaagc tatttctcta tttaaacgac atagtggttc
     7861 agataatgaa ctcttctgcc gaaggagcta agttcctttt cggaaggctt gcccttcctc
     7921 ccggaactga ggatgaacac gggcagtctt cccttggttt ttacctggca tttcaggcac
     7981 tacctactat aatctttttc tcagccctta tgtccatttt ctattactac gggataatgc
     8041 aaaaactcat aaaaggcttt tcatatctat ttacgaagtt gatgcgcata tccggagcgg
     8101 aatctcttag tgcagccagc aacatatttg tgggtgtgga gtctgctttt gccattagac
     8161 cgtttttgaa tcagatgaca aggtcggagc tttgcactgt tcttactgca ggaatggcaa
     8221 cagtatcatc aaacattctt gcactttata tattcatttt acaaagtgaa tttccaacca
     8281 tcgcatcaca cctcatatcc gcatcaattc tttccgctcc cgctgcaata gtaatgtcca
     8341 agatacttct acccgaatcc ggtaaacctg tgacacttgg attacaggta aatccctcgt
     8401 atgaaaaaga gagctctatt ttcgaatcta taattaatgg agcaaatggc ggggtaaaac
     8461 tgatagtcgg aatagcagcg cttcttgtag ccgtgctcgg acttgtagct ttattaaatc
     8521 tatttttaag tatgatagga actccaatga attcattatt tggtactgag ttcacattat
     8581 cccttcaaaa tattttcgga tatatctttt atccatttac attgattatt ggaatacccc
     8641 taactgatgc attcaacgta tctaaattaa ttggggaaaa agttattctt actgaagtag
     8701 taagctatca ggatctggca gttgcaataa aagatcaggt tattactaac ccaaggtcca
     8761 tagtggttac cacttatgca ctctgcggat ttactcacct tgcctcactc tcaatttttg
     8821 taggtggcgt gtctgcgatt gcccctgata gaaaactgga cattgcttca gtaggtttca
     8881 gagcacttat tgcagccacc cttgcctgcc taatgacagc ctgtgtttca ggcgtgttct
     8941 ttactggctc atcaatacta cttcaaaatt aacatctgcc cattattctt tccaataaaa
     9001 aatttaattt ttttaaaaaa gcccttgact taaatacaat aggtgtatat actcctatta
     9061 ttatgaaagg aaatgttaat gtgaacgaat taagtagata cgaaatagcc gagtgtgcac
     9121 aatgcacggg ctttaatctg aaaaaagcaa caagaataat acagaaccgt tttgaccagg
     9181 catttaaacc cgtaggcctc gaagggacac agtatacggt tctggcacat atctttgtac
     9241 atggaccaat atccctttca aaggttgcag aattaatgca tgtagacaga acaactttag
     9301 cacgtaactt agcaccttta gagagaaaag actttatcaa aattaagccc ggtatcgaca
     9361 ggaggtcaaa aattataagc attactgata aaggtatgga agttttagca aaagccctgc
     9421 cactttggaa aaaaacgcag gcagaaatta aagaaacatt aggaatagaa aattggagtt
     9481 cgatgttatc aaatttagaa aatttagtga ctgaaatgaa ataaaaaaat ttgaatctat
     9541 atgtgcatat acacatatat aaaaggaggt tagtaaaatg agtaaaccaa gaatactagt
     9601 tacatcggct gcaggacaca caggatcggt tgtggtaaag caactgcttg aaaaaggatt
     9661 tccagtacgt gcatttgtac gcagacatga cgcacggtct gaaaagcttc gcagagccgg
     9721 tgcagaaata tttgcaggag acctcttcga tatgagagac attaggtcag cactagtgag
     9781 tgtccagctt gcttactact gccctccctt tgcacctaac cttcttcacg gttcaatgct
     9841 ctttccactg gccgccgaag aagcaaagtt agaggtcgta gcactaatga gcggctggaa
     9901 cccacaccca tctcacccat cagttataac acgcgagcac tgggtaacta ataacatatt
     9961 tcagtggatg cctagtgtag atgtagttta tgttaacccc ggacttttcg cattcacata
    10021 cttttttggc cttccggcag tggctaactt tggaagactc atgcttccat acggtgaggg
    10081 tttaaacgct ccaccatcaa atgaggatat cgcatctgta gttagttcta tactgcaaaa
    10141 cccggctcca catataggaa agaactacag acctacaggg ccaaaactta tttccggaca
    10201 cgatgttgct gatatattag gtcaaattct cgaccgaaaa gtaacttatc aggacgtacc
    10261 cacatggatg ttcacaaagg ctgctaaggc acagggattc tcaaatttcg agattgccca
    10321 gatccgccac tatgctgaag aagtaagagg cggcacgttt gcagtaggag cgccaaccga
    10381 tcatgtagaa gtagtaaccg gacgtccggc agaggacttt gagacgaccg ctcgccgata
    10441 tatacagcac cctgaacttg taattcctaa cttcaagatt ggaggaaaga taggtgcagc
    10501 ctggcttatg atgaaaacaa tgctgaccaa ggctccttat ttagacaaat gggaatccga
    10561 gagaggctat ccgttgttag gctcacccgt gcttgctcat gacagtgaag agtggctaca
    10621 aagcgcaaaa aataaaaatc tggctcttat agaaaacaat caatcaatac taagagccgt
    10681 cgtctaaaga atgaaaatca aattttcata aaataatcca atatatagga ggtaagtaaa
    10741 atgagtaaaa agagatttga ggacaaggta gttttgatta caggcgcatc aggtggtatt
    10801 ggtaaagcat ccggtacaga ttttgccagg gaaggagcaa agcttgctct ggtatcaaga
    10861 tcaaaagaaa agcttgataa ggttgccggg catataaagc aatatacgaa tgaagttatt
    10921 actataccca ctgatgtatc gaaccaggat gaagtaaaca gtatggtatc tcaggtcatc
    10981 gacagatttg gaaacatcga ctatctgttt aacaacgctg gcagctcata tgttggggga
    11041 atagaaaaaa acgattttgc agaaaatgta aaaagaatgt ttgaggttga ttatatggat
    11101 acggtctaca caacaaaagc agttttaacc catatgaaaa agaagggctc aggtcacatc
    11161 atgaatatgt catcagtcgt tggaagaaaa gcatttccta agtttggcgg ttattccagc
    11221 ataatgcatg caataacagg tttttctgca gcattaagac aagagctcag agacagtggg
    11281 ataaaagtat ctacaattca ccccgctctc acacagacta atctgcttga tcatgtaaac
    11341 ccagaagaca tgcctccgcc ttttaagaat atgaccccaa tgccggtaga aaaagtatca
    11401 aaagcagtgc ttgatgggtt tcattataac cagacgagaa ttgtagttcc attccagccc
    11461 aagcttgttc ttttctggga tgcggtttca cctgcaattg gagattattt tgtaaggctg
    11521 ttatcaaaac ggactttttc tcgactaata ggaatgtata gaggatctct gtatcagcat
    11581 gacacggcag tataaacata tataatgaca tttaaaaatt agctgtaaaa aaaggagatt
    11641 taaatggatc ttgaaatctt atgggcaagc ggaagtcctt attcatggag cgtgctgctt
    11701 gccctggagg taaaaaaact tgaatataaa tccacactca tagagctttt aaagggtgag
    11761 ggcagaacac aggaatttct taaaatgaac ccgcgaggca aagtaccggt cttaagagat
    11821 gagcagacat atatcagtga atctcttgcg atacttagtt atttagacaa gaagtattcc
    11881 gaaccttcat tgtttgggaa tactcctcga gaggctgcag atataatgaa aattattagt
    11941 gattacaata gttatattgc tccagactta aaccacctta tttcgtctgc attttggagc
    12001 aagcccccaa accaggttgg attagaggaa gctaaacagg ctctttttta tgaactgtca
    12061 aaccttgaga agatagtttc tgaatcagag tggcttgcag gtgaaaacat ttctgcagcc
    12121 gatattatca tttttccttt tatgcagctt cttaatagat ctgaggctaa actacaaaaa
    12181 gaaatcggat acacgttgat gttcagtcat accaggtttc caaaaatgta cgagtggatg
    12241 agaaggtttg aagctttacc tggctatgag aaaacatatc ctcctcactg ggaacaggat
    12301 aaagggttga atcattatat ctcataaata taattcaatt ttttttaact caacattact
    12361 gcacaagcta ctatcagcac actgattacc acgtttaccc tagcaagcca tgatacaagc
    12421 tttttactct tattaagatc agacatttta gcaggatttg atttggaaga ttgcagtaat
    12481 ctaagatgtc tgggccctaa aataaaatcg tgagacaaac ttagcaatat cataataaca
    12541 aacaaaatca gttttattgc aagcacctta ccgaagtggg tagataataa acttcccgaa
    12601 gctatcatct catgggtgat tccgcggttt attacattaa ttagcccggt gatggcaaga
    12661 attataatcg ctatccagcc gatctttcta aatctcgtgg ccgttgccga aatcacttcg
    12721 gtgcgttttt cttgcggctg gaagtttcgg agtgcgggaa tgaccacaag cgcaataaat
    12781 aacattccgc cgacccacac tattgcggac aatacatgaa tcaaaaccac taggttatga
    12841 acatagaaca tactttatca cttaacaaca atttatattt gaccgtatta ggtcataaaa
    12901 cagttaattt tttccaatct taaatattga acgaaaagca gccctaaaaa ctttaaccaa
    12961 tacacgcggg cttaaaaata ttttggtaaa gtaccataaa tcatcatcac ttaaaacgat
    13021 acttccatac cacacaccca tttgggcgtt gagtgcaacg acatcttgtt caacacttac
    13081 actgtttatt gaaacatcaa aagtaccgga ttgtgttaga actttcacga ttgcgcctcc
    13141 gaattttctt ataaacaatt aaaacactat gttataatag tttcggaaaa taatataatg
    13201 tatataccat aaaagaaaag gcgaggaatc tcgccttttg ggacggtcct tcagaaccaa
    13261 aggatccatc tctaatactt cctgtcgctc ccacccaact gcttccctaa cctccattaa
    13321 taatataatc attcctattc tttataccag atcaaaacta cattttattt ctttaaacaa
    13381 tatatttttt aataccatga ctagaatggc tccggatata acgaaattct tatgttaaat
    13441 aaataccccg aatcctttta tatagtcatt tgtctaaata tataaataca aattaaggag
    13501 gtaactgaga tgaaaaagtt actaacatta acagcagttg cattatcact tgggctaagc
    13561 tcggcaagct ttgcagaatc taatgttttt ggtgtggata cacctcttac aaaacaggag
    13621 attagaagca acgtgcaagg cggcaaagtt caaaaaggat tgaatgatct ttatttatac
    13681 tctactgata gagttgagat aggttatcag gaagttacaa gcgaatctga taattccaac
    13741 tatatctttg gagtaaaaat taatgtatga gacaagcaat atcctccatc ctccttttat
    13801 gataactggg agcaggtatc aacatctgct cctggttttt tccattttaa ggcgtgttga
    13861 cactgatatc aaaaagtgct aactttctat gtaatgtcag ttgctggatc atctttacta
    13921 aaattcattc aattactggc aaataatttt tcaatagtta aggagcattt tacattatgg
    13981 cagaacgcga agttgggacc gttaaatggt tcaattccac caaggggttt ggatttatag
    14041 aacgcgaagg tggcaaagat gtttttgtac actatagtgc acttgaagga gcaggctacc
    14101 gctctttgga agaaggacaa aaagtagagt ttacagtagt tgaaggtgaa aaaggcccac
    14161 aggcacaaga cgtaacaaca gcttagtaat ttcatcaagc tgtcttatat cttcactttt
    14221 tatcatcatt caaaattaga ttaacagtaa atcttcgcct attttaccaa aaagctgatt
    14281 tgttttgtct ttgcaacctg cttaccctgc cttaacacct gcgcactaag tctgtaatca
    14341 cctcttttca ctgtccataa atgcttactg ccaataccag ttattttctc atcattaatg
    14401 ttccataaca cctcatcgtt atcttccact ccacgaatag aaaactcgag caactgagac
    14461 ttttcaggga tgcgtgggtc atatgcaata tgaaggttat ttgatggaat ctgtatcctt
    14521 attttttcct ttttttcctc ttttttcaca aattgggtag tattacggct tacaaacc
//
LOCUS       DBNGSV010000327         3243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_752168, whole genome shotgun sequence.
ACCESSION   DBNGSV010000327 DBNGSV010000000
VERSION     DBNGSV010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_752168"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1343)
                     /locus_tag="AC159U_11645"
     CDS             complement(78..1343)
                     /locus_tag="AC159U_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HIR3337747.1"
                     /translation="MANDFNKEERVAFEQILEGFQDAEVMSKNVSKYSTDQTMMERTN
                     DTIWRPQPYIMNSFDGQDQTANFNDKSQLAVPASINIEKSVPWIMSATEMRDALQEGR
                     LGTGAKQKLASDINVAILDVASLQGSLVVPVTGAATGFDDVALVEAIMNEQGVMDYDR
                     ILALSTRDYNGMAGDLAGRNDISAKKSLAAYERAYVGQVASFDTWKLDYAKRITAAGG
                     GAITMSTLDGATNYYVPKAVDLTQAGQKNVDNRFQNITVSATAGVVAGDAFTVATLEA
                     VHQITKEATGQLKTFRVVEVVDGTTLKITPPMITDQVSTEPTVQYQNCTIGTKGAASA
                     ITWLNINATAINPFWHKDAIEILPGRYAVPENAGAAVMRGKTDSGLELVMSKQFDINT
                     YKTKYRLDTRFGVVNKAPEMSGILLFNQP"
     gene            complement(1348..2280)
                     /locus_tag="AC159U_11650"
     CDS             complement(1348..2280)
                     /locus_tag="AC159U_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337748.1"
                     /translation="MRQGRYRMIIEDDDLVIDEVDNTEEENLEISEDSVNVESENEPA
                     TIQDEVDEDEEEDRIVTIGDSEPEPEEESEGEEEEKQKDPVVLVKKLRSVAKGLEKKN
                     KALQRQLEEKAKSVEVEEPIELGEEPTLKACGYDDVKYKQEIRAYDDKKKKVEEQAAK
                     KAKVVEEQTKAWKSRQEKYVNLKKEHSFKDFEDVEATVSETLSQAQQSMIVAGADDAA
                     LLVYALGKNPKKLKELADINDLATFAFKVAKLESQLKVTDKKAPKPETRVKTGKSGGI
                     SGSSDKVLERLREDAAKSGDYTQVTAYKNKLRKG"
     gene            complement(2290..>3243)
                     /locus_tag="AC159U_11655"
     CDS             complement(2290..>3243)
                     /locus_tag="AC159U_11655"
                     /inference="COORDINATES: protein motif:HMM:NF027825.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIR3337749.1"
                     /translation="NPMPTGPVGYTKPPQIPQALAALMQLVDIDTKELLGSTGEADKM
                     LSHVSGKAHEMIQKRIDGQAFIYMSNFSKAVRRTGEIWLSMAKEVYVEKGRKMKTVDN
                     MGQMGSIELLKTGIGKSGEVTDKNDLTKATFDVAVDVGPSSASQREATVSTVMGMLAI
                     TQDPETAQVLQSMAMMNMEGDGISDARDFFRKKLVQMGVIKPTPEEAQQMAEAAKNAK
                     PDPNALALEAMAAEAQAGAKKKEAEVFETMAKTEKIKADTLNTLDEIDLKKLQESRAY
                     IEQVEKIQQQRQQMALQQQAALQQQQAQQPENIEQQTPNAS"
BASE COUNT          811 a          775 c          545 g         1112 t
ORIGIN      
        1 agctcaatct tatcgttctt agccaatgtt ttctccttag tgtcaatgta aaaaggggag
       61 ttattctccc ctttttatta tggttgatta aatagaagga tacctgacat ttcaggagct
      121 ttattaacaa ctccgaaacg agtatcaagt ctatatttag ttttataggt attaatatcg
      181 aactgcttac tcataacaag ctcaagaccg gaatctgttt tccctctcat aactgctgca
      241 cctgcatttt caggaacggc atatcttcca ggaagaatct cgatagcgtc tttgtgccag
      301 aatgggttaa tcgctgtagc attaatattt agccatgtga tagcagaagc agcaccttta
      361 gttccaattg tacagttctg atactggaca gttggttcag tggaaacctg atctgtaatc
      421 ataggaggag taatcttaag agttgttcca tcaactactt caacaacacg gaaagttttt
      481 aactgaccag ttgcttcttt agtaatctgg tgtacagctt caagagtagc tactgtgaaa
      541 gcatccccgg caacaacacc tgctgtagca gatacagtaa tattctggaa tctgttgtca
      601 acgtttttct gccctgcctg agtaagatca actgctttag gtacataata gtttgtagca
      661 ccatcaagag tactcattgt aatagcacca ccaccggctg ctgttattct cttagcatag
      721 tccagtttcc atgtgtcaaa agatgcaacc tgaccaacat atgctctttc ataagcagcg
      781 agagatttct tagcagaaat atcatttcta cctgcaagat cccctgccat accattatag
      841 tcacgagttg aaagtgcgag gattctatca taatccataa caccctgctc attcataatt
      901 gcttcaacaa gagcaacatc atcaaaacct gttgcagcac cagttacagg aactactaaa
      961 gaaccctgaa gagaagcaac atcgagaatt gcaacattga tatcagaagc aagtttctgt
     1021 tttgcacctg ttccaagtct accttcctga agtgcatctc tcatttcagt tgcagacata
     1081 atccaaggta cagacttttc aatgttaatt gatgcaggta cagccaactg agacttatca
     1141 ttaaagtttg cagtctggtc ctgcccatca aaagagttca tgatataagg ctgtggtcgc
     1201 catatcgtat cattggttct ttccatcatt gtttggtcag tgctatattt actgacgttt
     1261 ttagacatta cttcagcatc ttggaaacct tccaaaatct gctcaaatgc tacacgttct
     1321 tctttattaa aatcatttgc catttaacta tcctttacga agtttattct tatatgccgt
     1381 gacttgagta taatcaccgc tttttgctgc gtcctctctc agtcgttcaa gtactttatc
     1441 tgaacttccc gatatacccc cggactttcc tgttttcacc cttgtctctg gtttaggtgc
     1501 tttcttatct gttactttca attgggactc caatttagcc actttaaaag caaaagtcgc
     1561 aagatcatta atatctgcaa gctcttttag tttctttgga ttcttaccca aagcataaac
     1621 taacagtgct gcatcatcag ccccggctac aatcatactc tgctgtgcct gtgataaagt
     1681 ttccgataca gttgcctcaa catcttcaaa gtctttaaag ctatgttcct tcttaagatt
     1741 aacatacttc tcttgacgac ttttccatgc cttagtctgt tcttcaacaa ccttggcttt
     1801 tttagctgcc tgttcttcaa ccttcttttt cttgtcatca taagcacgaa tctcctgctt
     1861 atacttgaca tcatcatagc cacatgcttt cagtgtaggt tcttcaccaa gttcaatagg
     1921 ttcttcaacc tctactgact tagccttctc ctcaagctgt ctttgtaaag ccttgttctt
     1981 tttctcaaga cctttcgcaa cactcctaag tttcttaacc aaaaccactg gatctttctg
     2041 cttttcttcc tcttcacctt cggactcttc ctctggttca ggctccgaat cgcctatagt
     2101 aacgatgcga tcctcttctt cgtcttcatc cacttcgtcc tggatagtcg caggttcatt
     2161 ttcggattca acattaacag agtcctcgga aatctcaagg ttctcttctt cagtgttatc
     2221 aacttcgtca ataacaagat catcatcttc aataatcatt ctataccttc cttgtctcaa
     2281 atgcaagcat taacttgcat tcggtgtttg ctgctctata ttctcaggct gttgtgcctg
     2341 ttgctgctgt agtgctgcct gttgctgtaa tgccatttgc tgtctctgct gctgtatttt
     2401 ctctacctgt tcaatataag cccggctctc ctgaagtttc ttgagatcta tttcatcaag
     2461 cgtattcaag gtgtctgctt taatcttctc agtttttgcc atagtttcaa atacttcagc
     2521 ttctttcttc ttggctcctg cctgtgcttc tgctgccatt gcttcaagag ccagtgcatt
     2581 aggatcaggt ttagcattct tagctgcttc tgccatttgc tgtgcttctt ccggtgttgg
     2641 tttaataact cccatctgaa caagtttctt tctaaagaaa tcacgtgcat ctgagattcc
     2701 atcaccttcc atattcatca ttgccattga ttgaagtacc tgtgcagttt cagggtcctg
     2761 tgtaattgcc aacattccca ttactgtact gaccgttgcc tctctctgtg atgcagaaga
     2821 tggtccaaca tcaactgcta catcaaatgt agctttagtc aagtcattct tatctgtaac
     2881 ttcaccactt tttccaattc ctgttttaag aagctcgata gatcccattt gccccatgtt
     2941 gtcaacagtc ttcatttttc gacctttttc aacatagact tcttttgcca ttgaaagcca
     3001 gatttccccg gttcttctaa cagcctttga aaagttggac atgtatataa aagcctgtcc
     3061 atctattctt ttctggatca tctcgtgagc tttcccggaa acatgagaaa gcatcttatc
     3121 tgcttctcct gtacttccaa gtaattcttt agtgtcaata tctactaact gcataagggc
     3181 agctaaagcc tggggaatct gtggaggttt agtatatcca accggaccgg taggcatcgg
     3241 gtt
//
LOCUS       DBNGSV010000328         1295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_60147, whole genome shotgun sequence.
ACCESSION   DBNGSV010000328 DBNGSV010000000
VERSION     DBNGSV010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_60147"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..916
                     /locus_tag="AC159U_11660"
     CDS             356..916
                     /locus_tag="AC159U_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3337750.1"
                     /translation="MKDQKGFTLIELLVVAAIIGILLALAIPNLLKARISSNEANARK
                     AMQVLRDAEALYANQDLNNDGVFNYTDIIGNITTTNSLICPATPCEPLDTLIDSSFEN
                     AVATGATAQCLDSKAGYCIQYDSALSGSTDFDFGWQTSMQAFNITGRKDFSVYGDGTI
                     RCTLSTSSTNTAGTFQADRNSHGCDD"
     gene            complement(921..>1295)
                     /locus_tag="AC159U_11665"
     CDS             complement(921..>1295)
                     /locus_tag="AC159U_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3337751.1"
                     /translation="MTKDMNIYSLGNDVASSLGVNIKKMELKIYFATSLAAAAVISVT
                     GPIGFVGLFIPHILRMIIGVDNRVLLPCSFLLGASFLTITDTISRTIISPVEIPVGII
                     TAFIGGIFFLWLLIRSKKEVLV"
BASE COUNT          433 a          233 c          226 g          403 t
ORIGIN      
        1 tgtcttgttt tttccatatt ttgcgtgtga aaaaattcgt gatatttttc ccactgttaa
       61 ttgccatgaa taccgcttta tcattgctct ttatcatata tactactcct aacaacgtct
      121 aatgcccaaa tttattatca tattcaataa ggtatcgaag gcacattcct tatcaaaatt
      181 gtaatagata ttattaccaa agtcatatta atagaagaga aatcatattt gacaatctaa
      241 tacttatgca ttaatataca acgtaagtat tatatattgt aagatttgtg ctatagagac
      301 cataaatcca gtatatgtta tgataattga taagcataaa agggggttgg tagttatgaa
      361 agaccagaaa ggttttacat taatagaact attagttgtc gctgcaataa taggaatatt
      421 attagcttta gcaattccaa atcttcttaa agcaagaatt tcttctaacg aagctaatgc
      481 aagaaaagct atgcaagtac ttagggatgc tgaagcacta tatgctaatc aggatctaaa
      541 taatgatgga gtatttaact atactgatat tataggaaat attacaacaa ctaattcttt
      601 gatatgcccc gctacgcctt gcgaaccgct agatacgctt attgatagca gctttgaaaa
      661 tgctgtggca actggagcaa ctgctcaatg tctggattcc aaggccggat attgtattca
      721 gtatgattcc gctttatcag gctcaacaga ttttgatttt ggatggcaaa cctcaatgca
      781 ggcctttaac ataaccggaa gaaaagattt ttcagtgtat ggagacggta caattcgctg
      841 cacattatca acaagctcaa ccaatacagc aggcaccttc caggcagacc ggaattcaca
      901 tggctgtgat gattagtagt ttagactaga acctctttct tacttctaat caagagccac
      961 aagaaaaata ttcctcctat aaaagccgta attatcccaa cgggtatttc aactggagag
     1021 ataattgttc tcgatattgt atctgtaatt gttaggaatg atgcacccaa gagaaatgag
     1081 catgggagaa gaaccctatt atcaacccca attatcattc taagtatatg aggaatgaag
     1141 agccctacaa aacctattgg tccggtgact gatattacag ccgcagcagc aagagacgta
     1201 gcaaaataga ttttcaactc cattttttta atgtttactc caagtgaaga ggcaacgtcg
     1261 tttcccaggg aatatatatt catatccttt gtcat
//
LOCUS       DBNGSV010000329        15057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1651984, whole genome shotgun sequence.
ACCESSION   DBNGSV010000329 DBNGSV010000000
VERSION     DBNGSV010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15057
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1651984"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="AC159U_11670"
     CDS             <1..1248
                     /locus_tag="AC159U_11670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3337752.1"
                     /translation="LHELLYQIINPKTMSKHVLQLNDKISANSNSKGGPEFWGLTKID
                     SLNRLAGGIAHDFNNLLTGILGNISLIKTNLNLDDSYYEKFVEIENISIRAKELTEKL
                     SVFSSGGQPYRSVQSVYDLLEQSLESVLKGSQVTYDIKREDEINTVSADPEQIKQVFS
                     NIILNSKQAVLGTGQLEIHLKNTVINDKTIMPLTEGDYIEISIKDKGYGIASQDLPKV
                     FDPYFTTRPDSAGLGLSIARTIISTHDGHIQIDSEEGIQTTVNIYLPVIKEKEKPAEP
                     QTTPEIESDGKLLSGKRVLVMDDEEFIRKVAGNILEHLGCQVEFAREGGEAIEKYRSH
                     VESDSRIDFVILDLTVPEGLGAEDTIKRLKEIDPDVKAIISSGYSHDPVMSNYKDYGF
                     IAMIRKPYEIEDFIKSFCELVEI"
     gene            complement(1254..1934)
                     /locus_tag="AC159U_11675"
     CDS             complement(1254..1934)
                     /locus_tag="AC159U_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3337753.1"
                     /translation="MLKQKNINKFVVISVLAHFVLLSVLALIFRSEAKVAISSFFEVS
                     TVKVASKSSNTQNNTESRKPVTSRAKTSITKKPISIKNKSRTKKETNQIKASPTEVRI
                     EQTSEADNKIAKKIKSSSKENNYQQVNIESINEDAYPDYSANPKPPYPLIARRRGYEG
                     TVLLKVLVLEDGKVGKVDIEKSSAFRVLDLAAINAVNKWNFIPGKQNGVQFASWVKVP
                     VKFRLKDY"
     gene            complement(2138..3448)
                     /locus_tag="AC159U_11680"
     CDS             complement(2138..3448)
                     /locus_tag="AC159U_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337754.1"
                     /translation="MKRLLLLLIIFTIIGVNTSYSSENITLSSLNLNNQTTTNCTDHL
                     IEYYHLQIQKSPSDYYNYDKLGQLHIRKARETGDLSLYDKAEQFLKKSLELNSNNYNS
                     YIFLGQVASSRHDFNKTLKYAKKAMEIDNKEASAYGIMGDAYIELGQYKQAQRAYEKM
                     VSLKSSLYSLSRLSHIKDITGDTDGAILEMKKSIRYGFRHRLPKENMAWAEVILGSLY
                     FNKGDLKNAEVHYLKSLSHLNNYYLALEHLAEINAVKGDYEKAIKIYQVVLEINPSPD
                     FHAAISGVYDKMGNKEKAEEHLKIAKDKYENYLDSGYLGYLEHHAAFYADNEIKLDNA
                     LELAHKDLEMKQDVFTYDTLAWVYYQLGNFKQAIKYSKKSLEHGTKDAGIYFHAGMIN
                     YSAGNLSDAKKYFDLCLKTNPYFDMSSADEVRNLIRQIEQSNAV"
     gene            complement(3539..5200)
                     /locus_tag="AC159U_11685"
     CDS             complement(3539..5200)
                     /locus_tag="AC159U_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HIR3337755.1"
                     /translation="MRTFQATKSSFILILFFVLSLTMGLNINTYGANHRESPISAIDD
                     EADIPDVFAFVSYDPNDPKASDKVTMIVTYDPFLEPGNGPTYFPFDEEILYAIRIDND
                     HDAKEDIIFEFKFKNEIRLPNVFTAYVGALDGINAPANAPADLDGVPTVGNPVVPPAI
                     TALDGPGSEGLGLRQSYTVTMVKNGKRTILNNINGGPLFKVPTNAGPRTMPDYSSLVE
                     QGIYQLPRGIKSFAGTVDDPFWLDLGAAFDSFNFRSQGFVVQGVLTEEQEADDTKNFA
                     SDEFSGYNVNAIAIEVPIKLLTSDGAKHASTVPEATIGVWGTSSRPTFKLFRNPGQKP
                     VLSGSYTQIQRMGAPLINELIIGIGSKDKFSMSEPRDDAQFSDFFFDPVIARVLNSVY
                     EALYGPDILPIPDVPRTDLAPLVQYVGPVAAEGTDPGPIADLLRLNTGIAPTAPENQS
                     RLGFLTLLDEDETNDDPAGFPNGRRVVDDVIDIVSRAAVGVLNPEFNIFPHNRIGDGV
                     NVNDVQPYRTSFPYLNDAHSGRDSRHIDPGDLGCEGTPTGECPVD"
     gene            complement(5443..5709)
                     /locus_tag="AC159U_11690"
     CDS             complement(5443..5709)
                     /locus_tag="AC159U_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337756.1"
                     /translation="MKKLLTVAAIGLALSMPVVGSADTNIFGVDTPIEKQHVSDQIKG
                     GKVEQDHISFYLQNQDQKDVVIDQDAQARDNISSSDLSVFGVKI"
     gene            complement(5771..6454)
                     /locus_tag="AC159U_11695"
     CDS             complement(5771..6454)
                     /locus_tag="AC159U_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3337757.1"
                     /translation="MLTYNSLNVDKSNSISTPGTNSVQHLLNIFYKEQKHNYKEYTDE
                     HLTKLYIENKDDEAINEIAFRYIDKIYRLTLSITKNSVDAEDVLQNIFLTLLEKIHYY
                     RGDSKFSTWIYRVSINATYSFLRKKNRDKASMESDSNTEHGTTIDHEDHSTIPDETNI
                     NKEILDIVRSSIDSLPEKYRIVIELRDIEGLSNQEAADITDITLTAFKSRLLRARNML
                     FEKLSTILK"
     gene            complement(6631..6957)
                     /locus_tag="AC159U_11700"
     CDS             complement(6631..6957)
                     /locus_tag="AC159U_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3337758.1"
                     /translation="MYVRENEGSWVEVVKGVQVKNLYTDQDRQYSTVLVKMEPGAAFP
                     DHKHAEDEECYIIEGDLNMGGHKFTSGDYIRADADSTHHSIYSENGCTLLVMASAKNE
                     MIEHHV"
     gene            complement(6948..7217)
                     /locus_tag="AC159U_11705"
     CDS             complement(6948..7217)
                     /locus_tag="AC159U_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337759.1"
                     /translation="MSDNGKIEELAALKTLELLEDEDNMAIDSLSSEDTALLESELDT
                     LYKAKEALLYNFTPESPRAELKNSILSKIKKNPLIKLLTTKDFCM"
     gene            complement(7217..7816)
                     /locus_tag="AC159U_11710"
     CDS             complement(7217..7816)
                     /locus_tag="AC159U_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3337760.1"
                     /translation="MTQIQTHDTASIPRGREDLEGLLAAISTGDEAAFSSFYDQTSSM
                     VYGLAKKILFDVEDSEEVMMEVYMQVWEKASQYDSSKAGPMTWLLMITRTRSIDKLRA
                     GSKRNTLEISSEEEIVEFGLTPEDEKYVNERKNIVNKALSMLGDKQRKAIELAYFYGL
                     SQSEIANEMNEPLGTVKSWIRLGMNKLREQMEIFKEELN"
     gene            complement(7970..8383)
                     /locus_tag="AC159U_11715"
     CDS             complement(7970..8383)
                     /locus_tag="AC159U_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337761.1"
                     /translation="MIKITSALILVSILIFTGLYSATASEQTKNDMVENPQNYLKITD
                     WQFYVAARVAIIHHVEIENTASIDYKNIRVKVDYYSNYPKYNRYLSSTSGVLPITVPA
                     NSSDIYVKGGTTLGAGSMGYIAKNIRIIGVEPVLE"
     gene            8646..13253
                     /gene="gltB"
                     /locus_tag="AC159U_11720"
     CDS             8646..13253
                     /gene="gltB"
                     /locus_tag="AC159U_11720"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR3337762.1"
                     /translation="MSKVGYPKKQGLYDPQFEHDSCGMGFVVNIKGKKSHTIVRQAIE
                     VLVNLQHRGACGCEANTGDGAGILIQMPDKFIRKAALKENIDLPEYKNYGVAMIFMPS
                     DSKQRGKIEKVFEKIVEDEGQKFLGWRTVPTDNSDLGNTAKSCEPVIRQAFIGRNPKI
                     KEDIDFERILYVIRSVSDTKIRYAGMKGGESYYLCSISYKTIVYKGMLTTNQLDPYYA
                     DLSDTDMQTAIALVHSRFSTNTFPSWDRAHPYRYVIHNGEINTLRGNINWMNARQAML
                     QSDLYGDDITKIFPIINPEGSDSSAFGNCLEFLTLSGRPIEHAVMMMIPEPWSRHESM
                     SEDRKAFYKYHSTMMEPWDGPASIGFTDGEKVGAVLDRNGLRPSRYYVTKDNLVILSS
                     EVGVLEVPAENVKYKGRLQPGRMLLIDTKQGKIVEDEELKSTLESEKPYARWLSEYMV
                     NINDIDEPDKEPETSDYEHSELLIRQRVFGYTFEELRVLIGPMAQNAVEPVGAMGVDT
                     PIAVLSDKPKLLYSYFKQLFAQVTNPAIDAYREELITDTRAHLGAEKDLINPEPESCR
                     RIELDDPFLTNKALNKIKYIDSEHFKSQTFPILFDPKKGGKGLGKALDKVFKDVDKAI
                     SNGINIVILSDRGFNKKKCPIPALLAVAGLNHHLIKNGNRMKVSIVLESGEPREVHHF
                     GLLIGYGANAINPYMAYETLNGMIKQNLYLENIDFDKAEANYNKSLVKGVIKIMSKMG
                     ISTVQSYHGAQIFEALGLDQDFIDKYFTWTPSRINGADIDVIAEEAVKRHSNAYPDRE
                     IKEDTLDEGGIYQWRHDGERHMWNPRTVHKLQHATRSNSYELYKEFAELSNNEEKEHF
                     TLRGLLDFRTDNDPVPIDEVESVEEICKRFKTGAMSYGAISKEAHEGLAIAMNRIGAK
                     SNTGEGGEDTDRFTPDDNGDLRRSAIKQVASGRFGVTSEYLVNSDEIQIKIAQGAKPG
                     EGGQLPGRKVYPWIAKVRLSTPGVGLISPPPHHDIYSIEDLAELIYDLKNSNKYARIN
                     VKLVSEVGVGTVAAGVAKGHADVILISGTSGGTGASPQSSIQHAGLPWELGIAETHQT
                     LLLNNLRSRVTLETDGQMKTGRDIAIAALLGAEEFGLSTAPLIVLGCIMMRVCHLDTC
                     PVGVATQNPELRKKYTGDPGHVVNYMKFIAAEMREYMAKLGFRTINEMVGRTDRLEMT
                     KALDHWKAKGLDLSKVFTKPDVPQDFGTYKQMEQDHGIQDSLDETVLMDICKPTIEKG
                     EPVEAIVPIKNINRTVGTIIGSELTRRYGLDGLPENTLKLHFNGSAGQSFGAFVPKSM
                     TLILEGDSNDYIGKGLSGGKIIVYPPKKSTFKAEENIIIGNVAFYGATAGEAYIRGMA
                     GERFCVRNSGVHAVVESVGDHGCEYMTGGSVVILGSAGRNFAAGMSGGIAYIFDKDKD
                     LETKLNTEMVSLEAINDNDQNTLKDRIEQHLEHTGSAVAKEILNTWSESIKKFKKVVP
                     NDYKRMIAHIKAAEQKGLGGEDALMAAFEENKRDLARVSGN"
     gene            13288..14772
                     /locus_tag="AC159U_11725"
     CDS             13288..14772
                     /locus_tag="AC159U_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR3337763.1"
                     /translation="MGNPSGFMEHKRELGKDRSPKKRINDWNEFHGHLPENGLKEQGS
                     RCMDCGVPFCQTGQIISGMTSGCPINNLIPEWNDLVYRGQWKEALERLHKTNNFPEFT
                     GRVCPAPCEGACVLGINDPAVTIKSIEVAIIDKGFEEGWVVPEPPKIRTGKKVAVIGS
                     GPAGLACAAQLNKAGHLVTVFERHDRIGGLLMYGIPNPHLEKSVVQRRVDLMAEEGVI
                     FKTNTEIGKELPAEILTRDFDAVVICTGATKPRDLDIPGRELKGIHFAMEFLHANTKS
                     LLDSNLEDGNYISAKDKDVIVIGGGDTGTDCVATSMRHGCNSLFQFEILGKPPLQRAP
                     ENPWPQWPRVYELSYGQEEAKAIFGDDPRHYQVSTVKFVGDDKGNVKELHTVDIKWGK
                     NDKGHLTMFDIPGTEKVWPAQQVFLALGFLGPEQPLIEELNVDTGPRSNIRAEYGKYM
                     TNIEGVFAAGDARRGQSLIVWAINEGREAARECDRYLMGSTDLP"
     gene            complement(14807..>15057)
                     /locus_tag="AC159U_11730"
     CDS             complement(14807..>15057)
                     /locus_tag="AC159U_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337764.1"
                     /translation="SELVLLIIDHPERMRDVLKLLSDLAISTEFQDNIFDYYLLNGAY
                     EDLDIKDVQYYWEGANKKNIEQIVLDRCKSWIEEYNKN"
BASE COUNT         4611 a         2755 c         3113 g         4578 t
ORIGIN      
        1 ctccacgagc tgttatatca gataataaat ccaaaaacta tgagcaaaca tgtacttcaa
       61 ctcaatgaca aaatatcagc aaattctaac tctaaaggtg gacctgaatt ctggggcttg
      121 acaaaaattg actcattaaa caggcttgca gggggtatag cgcacgactt taataacctg
      181 cttacaggaa tacttggaaa tatttcgctg atcaaaacaa atttaaattt agatgactct
      241 tattatgaaa aatttgtaga gattgaaaat atatctataa gggcaaaaga actaacagaa
      301 aaactttcag tattttcatc aggcggacag ccctacaggt cagtacagtc cgtttatgat
      361 ctgctcgagc agtccctcga gtctgtactg aaaggaagcc aggtgactta tgatattaaa
      421 agagaggatg aaataaacac cgtaagtgct gaccctgaac aaataaagca ggtatttagt
      481 aatataatat taaattccaa acaagctgtg ttgggtacag gacagctgga aatccactta
      541 aaaaatactg ttataaatga taaaactatt atgcctctta cagagggtga ttatatagaa
      601 atcagcatta aagataaagg ctatggaatt gcatcacagg atttaccaaa ggtttttgac
      661 ccttatttta caaccaggcc ggatagtgca gggcttggtc tttctatagc tcgtaccatt
      721 ataagcaccc atgacggaca tatacaaata gattcagaag agggtataca aactacagtt
      781 aatatatatc tgccagtaat taaagaaaaa gagaaaccag cagagcctca aacaacccca
      841 gaaattgagt ctgatggcaa actgctttca ggtaaaaggg ttttggtgat ggatgatgag
      901 gaatttataa gaaaagtcgc cggcaatata ttggagcatc ttggatgcca ggtggagttt
      961 gcaagagaag gtggggaggc tatagaaaaa tacagaagcc atgtagaatc tgatagtagg
     1021 attgattttg tgattctgga tttaactgtt ccagagggcc tgggtgccga agacacaata
     1081 aaaaggctaa aagaaattga cccggatgtt aaagcaataa tatctagtgg ttattctcac
     1141 gatcctgtta tgtctaatta taaagattat ggttttattg caatgattag aaaaccctat
     1201 gagattgaag attttattaa atcattttgc gagcttgtag aaatctaaat cggctaatag
     1261 tcctttagcc ggaattttac tggtacctta acccagcttg caaattgaac cccattttgt
     1321 ttgccgggta taaaattcca cttgttaaca gcatttattg ctgctaaatc caatactcta
     1381 aatgctgagc ttttttctat atcgactttg cctacttttc cgtcttcaag taccagcact
     1441 tttaacaaca ccgtcccttc atatcccctt cttctggcaa ttagagggta aggaggtttt
     1501 ggattagcac tataatccgg gtatgcatct tcatttatac tctctatatt tacttgctga
     1561 tagttgttct ctttggaact actttttatt ttttttgcaa ttttattatc tgcttcgctt
     1621 gtctgctcaa ttcgtacttc tgtaggagat gcctttattt ggttagtttc tttctttgtc
     1681 ctggatttgt tcttgattga aattggtttt ttcgtaatgc tagttttagc tctactggta
     1741 accggtttcc tggattcagt gttattttgg gtattactgc tctttgatgc aacttttaca
     1801 gtgcttactt caaaaaagct ggatatagca acttttgctt cacttctaaa gattaaagct
     1861 aaaacagata gtaaaacaaa gtgtgctagc actgatatta caacaaattt attaatattt
     1921 ttttgtttaa gcatagatgc cgactggaat ttataaatat attaacctat tcttcgtctt
     1981 aataaaacct gtttggttga tcggattatc tatgcgtaag tattaaaaaa ctttgttaaa
     2041 agcgttacta ttataagagt gtatagtgag gctctaacaa agtagagcaa gtaaacttcc
     2101 atgatattta cctccaacgg gatttatgaa attgccgtta aactgcattt gattgttcta
     2161 tttgtctgat taggtttctt acctcatcgg ctgagctcat atcaaaatat ggatttgttt
     2221 taaggcaaag atcaaaatat tttttcgcat cagataaatt acctgcactg taatttatca
     2281 ttcctgcatg gaaataaatc ccggcatctt tagtgccgtg ctcaagcgat ttcttggagt
     2341 acttaatggc ctgtttgaaa ttgccaagtt gataataaac ccaggccaga gtgtcatatg
     2401 taaaaacatc ctgtttcatc tccaggtctt tgtgcgcaag ttccaatgca ttatcaagct
     2461 ttatttcatt atcagcataa aaagcggcat gatgttccag gtatcctaaa taacctgaat
     2521 ccagataatt ttcatattta tcctttgcta tttttaaatg ttcctctgct ttttctttat
     2581 tgcccatctt gtcataaact cctgaaatag cagcatgaaa atcagggctt gggtttatct
     2641 ctagcacgac ttgataaatc tttatagctt tttcataatc tcctttaaca gcatttatct
     2701 ctgctaagtg ttctagggcc aaataataat tgtttagatg tgatagggat ttcaggtaat
     2761 gaacctctgc gttttttaaa tcacctttat taaagtagag tgaacctagt attacctctg
     2821 cccatgccat gttttctttt ggcagtctgt gtctaaatcc gtatctaatc gatttcttca
     2881 tttccaaaat tgctccgtct gtgtctccgg ttatatcttt tatatgtgat aggcggctaa
     2941 gtgaatatag acttgatttt aggcttacca tcttttcata ggccctttga gcttgtttat
     3001 attgcccaag ctctatatat gcatcaccca taattccata tgctgatgct tctttattat
     3061 ctatctccat agctttttta gcgtatttaa gagtcttatt aaaatcgtgt cttgatgatg
     3121 caacctgtcc taggaatata taagaattat aattattgct gtttagttct aatgattttt
     3181 tgagaaactg ctcagcttta tcgtacagag aaaggtcacc ggtttccctt gcttttctga
     3241 tatgcaattg tcctaatttg tcataattat agtaatccga tggagatttc tgtatttgta
     3301 aatgatagta ttcaatcaaa tggtcagtac aatttgtagt ggtttggtta tttagattta
     3361 agctgcttag tgttatattt tctgaactat aggatgtatt taccccaatt atagtaaaga
     3421 taattagtag taacagtaat cttttcattc gtttcctcca agatatagat atttaaaatt
     3481 agtaattata gtcaggggac aggagataat actcctgtcc cgatttaaga taagctattt
     3541 aatcaacagg acattcaccc gtaggtgttc cttcacaacc aaggtcaccc ggatctatat
     3601 gtcttgaatc tcttccacta tgagcatcat ttagatatgg gaaggatgtt ctgtatggct
     3661 gaacatcatt tacgtttaca ccgtcaccaa ttctgttgtg tggaaaaata ttaaactccg
     3721 gattaagcac acctactgct gctcttgaaa caatatcgat cacatcatca acaactctgc
     3781 gtccgtttgg aaagcctgca gggtcgtcat tagtttcatc ttcgtcaagt agtgttaaaa
     3841 aaccaagcct gctctgattt tctggtgctg taggtgcgat accagtgttc agacgaagaa
     3901 gatcggctat tggtcccggg tcagtacctt cggctgctac tgggcctaca tactgtacga
     3961 gaggagcaag gtctgttctt ggaacatcgg gtattggaag tatatccggt ccgtatagtg
     4021 cttcatatac tgagttcaat acacgtgcaa taactgggtc aaaaaagaag tctgaaaact
     4081 gtgcgtcatc tcttggctcg ctcatgctga atttatcttt agagccaata cctattataa
     4141 gctcatttat taaaggagct cccattctct gtatctgtgt atagctgcct gatagaacag
     4201 gtttctggcc tggatttctg aaaagcttga atgttggtct tgaagaagtt ccccaaacac
     4261 ctattgtagc ttcagggaca gtagaagcgt gttttgctcc atcgcttgtt agaagtttaa
     4321 ttggaacctc tattgcgatt gcatttacgt tatagcctga aaactcatca gaagcaaaat
     4381 tcttagtatc atcagcttcc tgttcttctg tgagtactcc ctgaacaaca aagccctgcg
     4441 acctgaagtt gaatgaatca aaagctgcac caagatcgag ccagaatgga tcatcgactg
     4501 tacctgcaaa tgattttatg cctctgggta gttggtatat gccctgttca acaagagatg
     4561 agtagtctgg catagttcta ggaccagcgt ttgtaggtac tttaaataga ggtccgccat
     4621 taatgttgtt aagaatcgtt ctctttccat tttttaccat tgtgactgta taagactgtc
     4681 tcaatcccag tccctctgaa cccggaccgt caagagcagt aatagcagga ggtacgacgg
     4741 ggtttccgac tgttggaaca ccatcgaggt ctgccggggc atttgcggga gcgtttattc
     4801 catccagtgc gcccacataa gctgtgaaca catttggtaa cctgatttca tttttaaatt
     4861 tgaattcaaa aattatatct tctttagcat catggtcatt gtcaatcctt attgcataaa
     4921 gaatctcttc atcaaaaggg aaatacgtag ggccattgcc tggctctaga aacggatcat
     4981 aggttacaat catggtaacc ttatctgaag cctttggatc atttgggtca taactaacaa
     5041 atgcaaatac atctggtata tcagcttcat catcgattgc tgatataggt gattctctat
     5101 ggtttgcgcc ataggtgttt atatttagtc ccattgtgag ggataaaaca aagaaaagaa
     5161 tgagaataaa acttgatttt gttgcttgaa aagttctcat atctttacct cctttaataa
     5221 attttatata catatacgga ggtactaagc ttattggatg aataatttat aaaatatttt
     5281 atatatcata aaaattattg ttaatatagt catatgcggg gacagtaaga ttaataacat
     5341 ataaatatga agaggtaata atagaaaata aaaaagccct ttacagtgca gtaaagggct
     5401 tttcttttag gaggatgggg ttgggggttt agtttttatg atttatattt ttacaccaaa
     5461 tactgataga tcggagctgc tgatattatc tcttgcctga gcatcctgat ctattacaac
     5521 atccttttga tcctgattct gcagataaaa gcttatatgg tcttgttcta ccttgccacc
     5581 tttaatctga tcacttacat gctgcttttc tattggagtg tccactccaa atatattggt
     5641 atcagcagaa cctaccactg gcatacttag tgctaatcca attgctgcta ctgttaataa
     5701 ttttttcatt ttactgcctc caattatgta tttatctatt tagacatata actaataata
     5761 acgattcata ttattttaaa atagtagata atttttcaaa aagcatgttt ctggctctta
     5821 ggagtcttga tttaaatgcg gttaaagtga tatccgtaat gtctgctgct tcctggttgc
     5881 tgaggccttc aatatctctc agttctatga caatcctata tttctccggc aatgaatcaa
     5941 ttgagcttcg tacaatatca aggatttctt tattaatatt ggtttcatcg ggaatagtac
     6001 tatgatcctc atgatctatt gtagtgccat gctcagtatt actgtcactc tccatgcttg
     6061 ctttgtctct gttctttttt cttagaaacg aataggtagc attaatgctt accctgtaaa
     6121 tccacgttga gaatttggaa tcgcctctat aatagtgaat cttttccagt agcgtaagga
     6181 atatattttg caatacgtct tctgcatcca cgctgttctt tgtaatagat aaagtaagtc
     6241 tataaatttt atctatataa cgaaaggcaa tctcatttat tgcttcatca tctttattct
     6301 caatgtataa ttttgtgagg tgctcatcag tatattcttt gtagttatgc ttttgctctt
     6361 tatagaatat atttagtaga tgctgcacgc tattagtgcc gggtgttgat atgctgtttg
     6421 atttatcaac atttaatgaa ttataagtaa gcatgtttat atcctcctta gcttaacttt
     6481 atgtatttgt tatagatata cgcaggagga tatgtgattg gatgaactta tttgaaaata
     6541 gtctgattaa taaggatagt ttaatcttga gtagaaaaaa ttaggataaa ataaaactgg
     6601 ttgattataa aactctagga tgcaagcaga ttaaacgtgg tgctcaatca tctcgttttt
     6661 agccgaagcc ataactaaga gagtacagcc gttttctgaa tatattgagt gatgtgtact
     6721 atctgcatcg gctcttatat aatcacctga ggtaaactta tgccctccca tattcaggtc
     6781 gccttcgata atataacact cctcatcttc tgcatgctta tgatcgggaa aagctgcgcc
     6841 tggctccatc tttacaagca ctgtagaata ttgtctgtcc tggtctgtgt ataaattttt
     6901 tacttgtacg cctttaacaa cctctaccca ggagccttca ttttctctta catacaaaaa
     6961 tcctttgtcg tgagaagttt tatcagtggg ttttttttaa tttttgaaag tatactgttt
     7021 ttgagctcag ctcgcggact ttcaggagta aaattataga gtagcgcttc ttttgcttta
     7081 taaagcgtgt ctaattcaga ttccaataaa gcggtgtctt cagaagataa tgagtctatt
     7141 gccatattat cttcatcttc caacagctcc agtgttttaa gtgccgctaa ttcttcaatc
     7201 ttaccgttgt ctgacattaa ttaagttcct ctttaaatat ctccatctgt tctctgagtt
     7261 tgttcatgcc aagccttatc cagctcttta ccgttcccag aggctcattc atttcatttg
     7321 ctatctcact ttgactgagt ccataaaaat atgcaagctc aattgccttc ctttgcttgt
     7381 ctcctagcat tgaaagtgcc ttattaacaa tattttttct ctcatttaca tatttttcat
     7441 cttccggtgt taaaccaaac tcgacaatct cttcttctga gctgatttcc agtgtatttc
     7501 tctttgatcc tgctcttaac ttatctattg accttgttct ggtaatcata agaagccatg
     7561 tcatgggacc ggcttttgac gaatcatact gtgaagcttt ttcccatacc tgcatatata
     7621 cttccatcat tacttcctct gaatcttcaa catcaaacag tatttttttt gcaagaccat
     7681 aaaccatgct gcttgtctgg tcataaaaac tactaaacgc agcttcatcc cctgtggata
     7741 ttgctgccag taaaccttca aggtcttccc tacccctggg gatactagcc gtatcgtgtg
     7801 tttgaatctg tgtcatagca caattgcata aataattact gtacggatta acattatata
     7861 atattccgct gtatttggaa ataccatctg tcaatattga tacggcgtaa ctgttcacgt
     7921 acatatatac gtaaactatt accgttttgg atgaaaataa tgataaaatt tattccaata
     7981 caggctcaac accaataatt ctaatgtttt ttgcaatata tcccatcgat ccagcaccga
     8041 gtgtggtacc gcctttaaca taaatatcac tcgaatttgc aggtactgtt attggaagta
     8101 ctccgcttgt gctgcttaaa tatctattgt atttggggta gtttgaataa taatccacct
     8161 taaccctgat attcttataa tctatactag ccgtattttc tatttctaca tggtggataa
     8221 tagcaaccct tgcggcaaca tagaactgcc aatcggttat ttttaggtag ttttgagggt
     8281 tttctaccat atcgttcttt gtttgttcag aagctgtggc actgtataat cctgtaaaaa
     8341 ttagtataga tactaatatt aaagcagagg ttatcttgat cattataggg ccccccagag
     8401 gattcaatct agattcaata tagatattgt agcagaaaaa taatcaaatg ggggcgatta
     8461 tagtgttacg gagctaaaaa gtactttaaa tataagaaaa tatcagtata atctataggc
     8521 tcggctgaat actgccagta acggtgtttc tttaggattc atttcctggg tagaaaatgt
     8581 aaaaagtagt aataatttat ttactaaggt attcagaatt atattcttta taggtgattt
     8641 gttatatgag taaagtcgga tatccgaaaa aacagggttt atatgatcca cagtttgaac
     8701 atgactcatg cggaatgggt tttgttgtaa acatcaaggg caaaaaatct catacaattg
     8761 taagacaggc aattgaagtg ctggtaaatc tacagcacag gggagcgtgt ggctgtgaag
     8821 ctaacactgg tgatggagca ggaatattaa tccaaatgcc tgacaaattt atacgaaaag
     8881 cggctttaaa ggaaaacata gaccttccgg agtataaaaa ttatggcgta gctatgatat
     8941 ttatgccttc tgacagcaag caaagaggca aaattgaaaa agtatttgaa aaaatcgtag
     9001 aggatgaagg acagaaattc ttaggctgga gaactgttcc aactgacaac tctgatcttg
     9061 gaaatacagc aaaatcctgc gagccggtta taaggcaggc ttttatcgga agaaacccga
     9121 aaataaagga agatatagat tttgaaagaa tactatacgt aataagaagt gtatccgaca
     9181 ctaaaattag atatgcaggt atgaaaggcg gagagagcta ctatttatgc agcatctcct
     9241 ataaaacgat agtttataaa ggaatgctca ctacaaatca gctcgatccc tactatgcag
     9301 acttaagcga tacggatatg caaaccgcta ttgctcttgt gcattcaagg ttcagcacca
     9361 acacattccc aagctgggac agggctcatc catacaggta tgtaattcat aatggtgaaa
     9421 ttaataccct caggggaaat ataaactgga tgaatgcaag gcaggcaatg cttcagtctg
     9481 atttgtatgg cgatgacatt acaaagatat tccccataat taatcccgag ggcagcgact
     9541 catcggcatt tggaaattgc cttgagtttc ttacactttc aggaagacca attgaacatg
     9601 cggtaatgat gatgattccc gagccctggt caaggcatga aagtatgtcg gaagatagaa
     9661 aggcatttta taaatatcac agcacaatga tggagccatg ggacgggcct gcatcaatcg
     9721 ggtttacaga tggagagaaa gttggggcgg tgctcgatag aaacggtctc aggccatcaa
     9781 gatattacgt gacaaaagac aatcttgtta ttttgagctc cgaggttggg gttttagaag
     9841 tgccggctga aaatgtaaaa tataaaggac gccttcagcc cggaagaatg ctccttattg
     9901 ataccaaaca gggcaagatt gtagaagatg aagagctaaa gagtacactt gaatcagaga
     9961 agccttatgc tcggtggctc tccgagtata tggttaacat taatgacatc gatgaacctg
    10021 ataaagaacc agaaacatcg gattatgaac attctgaact ccttataagg cagagggttt
    10081 ttggatatac atttgaagag ctaagggtgc ttattggtcc aatggcacaa aatgcggttg
    10141 aacctgtagg ggccatgggg gtagacacac cgattgcagt attatcggat aaaccaaaac
    10201 tactttacag ctattttaaa cagctatttg cccaggttac aaaccctgca atagatgcat
    10261 acagagaaga acttattacc gatacaaggg cacatcttgg cgcagaaaaa gaccttataa
    10321 atcctgagcc tgaaagctgt agaagaattg agcttgatga tccctttttg acaaacaaag
    10381 cgctaaataa gataaagtac atagacagtg aacatttcaa gtctcagact tttccaatac
    10441 tgtttgatcc taaaaaaggc ggaaagggct tgggtaaggc gcttgataag gtgtttaaag
    10501 atgtagacaa agctatatcg aacggcatta acattgtgat attgtccgac aggggattta
    10561 ataaaaagaa atgccctatc cctgcacttt tagcagttgc tggtcttaac caccatttaa
    10621 tcaaaaacgg aaacaggatg aaagtttcca ttgtactcga atcaggtgag ccaagggaag
    10681 tacatcattt tggtctgctg atcggatacg gcgcaaatgc tattaatcca tatatggcat
    10741 acgaaacact aaacggcatg ataaagcaaa acctttatct cgaaaatata gattttgata
    10801 aagccgaggc taattacaac aagagtcttg taaaaggtgt tattaagata atgtccaaga
    10861 tgggcatttc tacggtacag agctatcacg gcgcccagat atttgaggcc cttgggcttg
    10921 accaggactt tatagataaa tatttcacat ggactccatc acgtataaac ggggcagaca
    10981 ttgatgtgat tgctgaggaa gctgtaaaga gacactcaaa tgcttatccg gatagagaaa
    11041 taaaagaaga tacacttgat gaaggtggca tctatcagtg gaggcacgat ggcgagcgtc
    11101 atatgtggaa ccccagaaca gttcacaaac tacagcatgc cacaagaagt aacagctatg
    11161 aactttataa agagtttgct gaactatcca ataatgaaga aaaggaacat tttacgctta
    11221 gaggattatt agattttaga accgataacg accccgttcc aatcgacgaa gttgagtcag
    11281 tcgaagagat atgcaagagg tttaaaacag gcgccatgtc atacggtgct atcagcaaag
    11341 aagctcacga agggcttgcg atagccatga atcgtattgg agcaaagagc aacacaggcg
    11401 agggcggaga ggatacagac aggtttaccc ccgacgacaa tggtgacctt agaagaagcg
    11461 caatcaagca ggtggcatcg ggtaggtttg gagtaacaag tgaatacctc gtaaattcag
    11521 acgagataca gataaagatt gcccagggag caaaaccagg cgaaggcggg cagcttcccg
    11581 gaagaaaagt ttacccatgg attgccaagg taagactatc aacccctggt gtagggttaa
    11641 tctcacctcc acctcatcac gacatatatt caatcgaaga tttggcagaa cttatctacg
    11701 atcttaaaaa ctcaaataaa tacgccagga ttaatgtaaa acttgtatca gaagtaggtg
    11761 ttggaaccgt tgctgcaggt gtggccaagg gccatgctga tgtgattctt ataagtggta
    11821 catctggtgg tacaggcgca tcacctcaga gcagtatcca gcacgcgggg cttccctggg
    11881 agcttggcat tgccgagact catcagacac ttcttcttaa taatcttaga agcagggtca
    11941 cgctcgaaac agacggacag atgaaaacag gaagggatat tgcgatagct gctcttcttg
    12001 gagcagagga atttggactc tctactgccc cactaatagt tcttggctgt attatgatga
    12061 gagtctgtca tctggatacc tgccccgtgg gagtagcaac tcagaatcct gaacttagaa
    12121 aaaaatatac aggcgacccc ggccatgtgg ttaactatat gaaatttatc gctgctgaaa
    12181 tgagagaata catggcaaag cttgggttta gaaccataaa cgagatggta ggtagaacag
    12241 ataggcttga gatgacaaag gctcttgatc actggaaagc caagggactc gatctgtcaa
    12301 aggtattcac taaacctgat gttccccagg attttggaac atacaagcag atggaacagg
    12361 atcatggtat tcaggactct ttggatgaaa cggttcttat ggacatatgt aaacccacaa
    12421 tagaaaaagg tgagcctgta gaagccattg ttccaataaa aaatattaac agaacagtag
    12481 gcacaatcat aggaagtgag cttacaagaa gatatggcct ggacggcctg ccggaaaaca
    12541 ctctcaagct gcattttaac ggctcagccg ggcagagctt tggtgcgttt gtgccaaaga
    12601 gtatgacatt aattctcgaa ggcgattcaa atgactatat aggtaaaggt ctttcaggcg
    12661 gcaaaattat cgtttatccg ccaaagaaat ccacatttaa ggccgaagaa aacattatta
    12721 ttggaaacgt cgcattttat ggagccactg cgggagaggc ttatattaga ggcatggcag
    12781 gggagaggtt ttgcgtaaga aacagcgggg tacatgcagt ggtagagtct gttggtgatc
    12841 atggatgcga atacatgaca ggcggaagcg tggtgattct tggatctgcc ggaaggaact
    12901 ttgcagccgg tatgtctggt ggaattgcgt atatatttga taaagataag gacttggaaa
    12961 caaagctaaa taccgagatg gtgtcgcttg aagcaataaa tgacaacgat cagaacactc
    13021 ttaaagacag aatagaacaa cacctggagc acacgggtag tgcagttgca aaggaaatat
    13081 tgaatacatg gtctgaaagt attaagaaat ttaaaaaagt cgtgccgaat gattataagc
    13141 gtatgatagc tcacattaaa gctgctgagc agaagggtct gggaggagag gatgcgctta
    13201 tggccgcatt tgaggaaaac aagagagacc ttgccagagt aagcgggaat taatttgata
    13261 tcaagatgat tttggaggtt ttgttgtatg ggtaatccct cgggatttat ggagcacaaa
    13321 agggagcttg gcaaagacag gtcgcccaaa aaaaggataa atgactggaa tgagtttcac
    13381 ggacatttgc ctgaaaacgg actaaaagaa caagggtcaa gatgtatgga ctgcggcgtg
    13441 cctttttgtc agactggtca aataataagt ggtatgacat cgggatgtcc gattaataat
    13501 ctaattcctg aatggaacga ccttgtatac aggggtcagt ggaaagaggc gctcgagagg
    13561 cttcataaga caaataattt tccagagttt actggcaggg tgtgccctgc tccatgcgag
    13621 ggagcatgtg tgcttgggat taacgatcct gctgtaacaa ttaaaagtat tgaagttgct
    13681 attatcgata aaggatttga agaagggtgg gttgtacctg aaccaccaaa gataagaacc
    13741 ggtaagaaag tagcggtaat aggctcaggg ccagcggggc ttgcctgcgc agctcagctt
    13801 aataaagccg gtcatctggt aactgtattt gagcgtcatg acaggattgg cggtctttta
    13861 atgtacggta ttccaaatcc tcatctcgaa aagtctgtag tacagagaag ggtggacctt
    13921 atggcagaag aaggggtgat ttttaagaca aacacagaga tcggaaaaga acttcctgcc
    13981 gaaatattaa cccgagattt cgatgcggtt gtaatctgta caggagcaac caagccaaga
    14041 gatttggata ttcccggaag agaactaaaa ggtattcact ttgccatgga gtttctgcat
    14101 gcaaacacca agagcctttt agacagtaac cttgaagatg gaaattacat atctgcaaaa
    14161 gataaagatg taattgtaat aggcggaggg gacacgggaa ctgactgtgt tgcaacttcc
    14221 atgaggcacg gatgcaatag cctctttcag tttgaaatac ttggaaagcc accgctgcag
    14281 agagcgcctg aaaatccatg gccgcagtgg ccccgtgtat atgagcttag ctacggtcag
    14341 gaagaggcaa aggcaatatt tggtgacgac ccgaggcatt atcaggtatc tacggttaag
    14401 ttcgttggcg atgataaagg taatgtaaaa gagctgcata cggttgatat taaatggggc
    14461 aagaacgaca aaggccatct gactatgttt gatattcccg gtactgaaaa ggtatggcca
    14521 gcgcagcaag tgtttttggc gctaggattc ttaggcccgg aacagccgct cattgaagag
    14581 cttaatgtgg ataccggccc aaggtcaaac ataagggcag agtatggtaa gtacatgacc
    14641 aatattgagg gagtatttgc cgcgggtgat gcaagaagag gtcagagcct tatagtctgg
    14701 gcaataaacg aaggcagaga agccgcaaga gaatgcgacc gttaccttat gggcagcact
    14761 gatttgccgt aagaattaga aggctatcaa tcatttttct gatctactaa tttttgttat
    14821 attcctcaat ccagctttta catctatcca acactatttg ttctatgttc ttcttatttg
    14881 ctccttccca ataatattga acatccttaa tatctaaatc ttcataagcc ccattaagaa
    14941 gataataatc aaaaatgtta tcctgaaatt ctgttgatat agctagatct gataataatt
    15001 ttaatacatc tctcatacgc tcgggatggt caataattaa tagaactagt tcacttg
//
LOCUS       DBNGSV010000330         4856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1652952, whole genome shotgun sequence.
ACCESSION   DBNGSV010000330 DBNGSV010000000
VERSION     DBNGSV010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1652952"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..378)
                     /locus_tag="AC159U_11735"
     CDS             complement(94..378)
                     /locus_tag="AC159U_11735"
                     /inference="COORDINATES: protein motif:HMM:NF037617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337765.1"
                     /translation="MSTKKQREEKLKQAKEILRVLGMPKTQYNDRSGWVLLALADIRP
                     EISWDKAKSPLLATVDIMQFIREYYKQNYKPNSRETIRRHDLAPYNSTRL"
     gene            complement(378..1868)
                     /locus_tag="AC159U_11740"
     CDS             complement(378..1868)
                     /locus_tag="AC159U_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HIR3337766.1"
                     /translation="MSNNNAVEQLSLFDLKSRSQSKELGQFFTESKTADYMASMLHSV
                     GNVELVKILDAGAGEGLLTQSAALHCLELGYSKVHAVLYEIDEDILPILQKNMDQLVR
                     IFIKKGGKLSYEIINEDFVLSRPDKNEPNFHISIINPPYFKYNSKHSIYSGATEDLFK
                     GNPNIYASFMAVVSSCLVSLGQMVAIVPRSFTNGLYFKGFRKYLQSQMSLEQAHIFQS
                     RNRLFKNMGVLQENIICKFVKSEQKPTIKISSSTCSEDLAESEAASYPQNLIIDRTNL
                     HNIIRIPESAADGHILRIVENWPSSFEKNNYYISTGPVVEFRTREFITTKAQKHSIPL
                     LRMHNVKAFRIEWTGTHRKDTHFILKEGHEKHTCENRVYVIVKRFSSKDEKRRLVAGI
                     NDPEQFKDHLIGLENHLNYIGVKGEDMNLPEAFGLAALFNSTFMDRYFRSISGNTQVN
                     ATEMRLLKLPRRDQIMTIGKEVQRVKSYEQSAVDEVVQSALNIGHI"
     gene            1963..2631
                     /locus_tag="AC159U_11745"
     CDS             1963..2631
                     /locus_tag="AC159U_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dph6-related ATP pyrophosphatase"
                     /protein_id="HIR3337767.1"
                     /translation="MPKQIAVSWSGGKDSTLALNEIVNNTDYEVRSLITTVTEGYDRI
                     SIHGVRTELLEQQADSIGIPLQKVYIPKNSTNEQYESALKSVLLKFKKEGINEIVFGD
                     IFLEDVKRYRDDLLATPDMKGIYPIWKKDSRELAERFIDLGFKAVTTCVDSQQLDKSF
                     VGREYNYEFIDDLPDTADPCGENGEFHTFVYDGPLLNNKINFDKGEIVFRDNRFYYCD
                     LLPD"
     gene            2672..4129
                     /locus_tag="AC159U_11750"
     CDS             2672..4129
                     /locus_tag="AC159U_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3337768.1"
                     /translation="MDKDKFISDLEGICGKEYTISEHSELVAFECDGLTGYRVKPFCV
                     VLPATTGEVSEVIKLCYDNDVPFVPRGSGTGLSGGALPVDDGIVISLTRMNNILDVDI
                     ENQRVVVEPGVINVQVTKAVEQWGYYYAPDPSSQVVCSIGGNVAENSGGVHCLKYGVT
                     SNHVLGIEAVLPTGEVVELGGKTLDSPGYDLLGLFVGSEGLLGIATKITLKIIRKPQT
                     IKTMFASFGNIAQAGASVSGIISKGIIPGGMEIMDNISIRAVESVVNSGYPVDAAGVL
                     IVELDGPDAEVEAQTPLVIEVLKENNAEVIKIAKDMAERDLFWKGRKSAFAAMGRMSP
                     EYYVQDGVIPRSKLAEVLAEIEQLSKKYGIRVANVFHAGDGNLHPLILYDTRIEGELE
                     KAELIAGDILEICVNAGGSITGEHGVGFDKKRFMDKMFSEDDLDTMNMIRCTFDHKNL
                     CNPGKVFPTPRTCVEPGMKKFTPHELHENGTAEMY"
     gene            4107..>4856
                     /locus_tag="AC159U_11755"
     CDS             4107..>4856
                     /locus_tag="AC159U_11755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3337769.1"
                     /translation="MVQLKCTDPRQVIMDKALKDIENVVGAKYLSTGEQALSSYRVDL
                     KTPVAVIYPGTDLEISEIIKICSTHKLSLTPYGNGTKISTGNTPNKLELVLSTSRLDK
                     IIDHGSDDLIATAEAGITLQEFQAVLKKKNQQVAVHPPNQKEGCTLGGMINTNDYGPS
                     RLRYGSVRENLLALKFVRADGKIVKGGAKVVKNVAGYDIPKLMTGSLGTLGIITEATF
                     RLYPQQPASNTIIANINNISQINEINNKILNS"
BASE COUNT         1527 a          916 c          985 g         1428 t
ORIGIN      
        1 tcttaaatat cttattatct gctctggtgt gtaacgcttt ctgggcattt cccttgcctc
       61 ctattcatag ggtttattac ctaaaattct aacttataat ctggtactat tatatggggc
      121 aaggtcatgg cggcggatag tttcacgact atttggtttg taattctgct tgtagtattc
      181 acgtatgaac tgcattatat ctactgttgc tagcagtggt gacttagctt tatcccaaga
      241 aatctctggt ctaatatctg ctaaagctaa taatacccat cctgatctat cgttatattg
      301 tgtttttggc ataccaagaa ctcttaatat ttccttagcc tgttttagct tctcctctcg
      361 ctgtttttta gtgctcatta gatgtgccct atattaagtg cggattgaac gacctcatcg
      421 actgctgatt gttcatagct tttcactctc tgtacttcct ttccaattgt cattatctga
      481 tctcttcttg gtaatttcaa tagtctcatt tctgtagcgt taacttgggt atttccagat
      541 atagatctaa aataacgatc cataaacgta gaattaaata acgcagctaa cccaaacgct
      601 tccggaagat tcatatcttc accttttact ccaatatagt ttaaatgatt ttctaatcct
      661 attaagtgat ctttgaattg ttcagggtca ttaattcctg cgacgaggcg acgtttctca
      721 tctttagaac taaagcgttt tacaattaca taaactctgt tttcacatgt gtgcttctcg
      781 tgtccttcct ttaaaatgaa atgtgtatct tttctgtgtg ttccagtcca ttctattcgg
      841 aacgctttaa cattatgcat tcgtaataaa ggaatgctat gtttttgagc cttagttgta
      901 ataaattctc tggtacgaaa ctcaacaacc gggccagtgg atatataata gttgtttttc
      961 tcaaagctgc ttggccaatt ttctactatc ctcaaaatat gaccatcagc ggcagactct
     1021 ggaatcctta taatgttatg tagattggtt cgatcaataa taaggttctg tggataactt
     1081 gctgcctccg attctgctaa gtcttctgag catgtgctcg aagatatttt tatagttggc
     1141 ttctgttcac tttttacgaa cttacaaata atgttctctt gcaaaactcc catatttttg
     1201 aaaagccgat tccttgattg aaaaatatgt gcttgttcta agctcatctg agactggaga
     1261 tatttacgga aacctttgaa atacaggcca ttagtaaaac tacgtggaac aatggctacc
     1321 atctgtccta gtgacaccag gcatgaagaa acaacagcca taaaagaagc atatatatta
     1381 ggattccctt taaacaaatc ctctgttgcc ccactatata tcgaatgttt ggaattatac
     1441 ttgaaataag gtggattgat tattgagata tgaaagttag gttcgttttt atctggtctc
     1501 gacaatacaa aatcctcatt aataatttca tatgaaagtt ttccaccctt tttgataaat
     1561 attctgacca attgatccat gtttttttgg agaattggca atatatcctc atctatctcg
     1621 taaagaaccg catgcacttt actataccct agctctaagc agtggagagc tgctgactgt
     1681 gtaagaagcc cttctcctgc accagcatca agaattttta ccaactctac gttaccaaca
     1741 gagtggagca ttgatgccat atagtcggct gttttagact ctgtaaagaa ctgtccaagc
     1801 tctttacttt gtgatctgga cttaagatca aataaactaa gttgttctac agcattatta
     1861 ttcgacacaa tatagatatc ctattaattt atatgttaaa aaagctttaa tagatttaat
     1921 ttattataat tctaatactt aatatattaa atacaattga ttatgccaaa acaaatagca
     1981 gtttcctgga gtggtggaaa ggacagtaca ttagctctta atgaaatcgt taataacaca
     2041 gattatgagg ttcgatcttt aataaccact gttacggaag gttatgacag gattagtatt
     2101 catggtgtga ggactgagct attggaacaa caggctgatt cgattggtat ccctttgcaa
     2161 aaggtttata ttccaaaaaa ctctacaaac gagcagtatg aatcggcact caaaagtgtt
     2221 ctacttaaat ttaaaaaaga gggaataaat gaaatagtgt ttggagacat atttttagaa
     2281 gatgttaaaa ggtataggga tgatctattg gcaactcctg atatgaaagg catctatccg
     2341 atatggaaaa aggattccag agagcttgct gaaagattca tagatcttgg cttcaaggct
     2401 gttacaacat gtgtagattc acaacagtta gacaagagtt ttgtgggaag agaatataat
     2461 tatgaattta tagatgacct tccagataca gcagacccct gtggagaaaa cggcgagttt
     2521 cacacctttg tctacgacgg cccattactc aacaacaaaa tcaacttcga taaaggtgaa
     2581 atagtattca gagacaacag gttttattat tgtgatcttt tgccagatta aaacattgaa
     2641 ttttcaccac tatataatat gatcttgtgc gatggataag gataaattca tttctgattt
     2701 agaggggata tgtgggaagg aatatacgat atctgagcac tccgagcttg ttgcgtttga
     2761 atgcgatggg ctcacgggat acagggtaaa acctttttgt gtggtcctgc ctgctactac
     2821 aggcgaagta tctgaagtta taaaactgtg ctacgataac gacgtgcctt ttgtaccaag
     2881 gggctcaggc accgggctat ctggcggcgc ccttcctgtt gatgacggaa tagtaatctc
     2941 acttacaagg atgaacaaca tcctggacgt tgatattgaa aatcagcgtg ttgtggtaga
     3001 accgggggtg ataaatgttc aggtcacaaa agccgtagaa caatggggat actactatgc
     3061 acccgaccct tcgagtcagg tagtgtgcag cataggtggt aatgtagccg aaaattcagg
     3121 cggtgttcac tgcctaaaat acggtgttac ttcaaatcat gtgctgggaa ttgaagccgt
     3181 acttcctaca ggagaagtag tagagcttgg tggaaagaca ttagactcac cgggttatga
     3241 tttacttgga ctatttgtag gctccgaggg tcttttaggt atcgctacta aaatcacgct
     3301 taagataata cgaaaaccac agacaataaa aacaatgttt gcttcttttg gaaatattgc
     3361 ccaagccgga gcatctgtat caggaatcat atccaaaggc ataatcccgg gtggcatgga
     3421 aattatggac aatattagta tacgggcagt agaaagcgtt gtaaactctg gctacccggt
     3481 tgatgctgca ggagtgttga ttgttgagct tgacggtcct gatgctgaag tcgaagccca
     3541 gacaccgctt gttatagaag tacttaagga aaataatgca gaggtaataa aaatagctaa
     3601 agacatggct gagagggacc tgttctggaa aggaagaaag agcgcctttg ccgctatggg
     3661 caggatgagc ccggaatatt atgtacagga cggtgtcata ccaagaagca aacttgcaga
     3721 ggttctggca gaaatagagc agcttagcaa aaaatatgga ataagagttg ctaatgtatt
     3781 ccacgcagga gacggaaatc tgcatccatt aatattgtat gatacaagaa tagaaggtga
     3841 gctggaaaaa gccgagctca ttgcaggaga catcttggaa atttgcgtaa acgccggggg
     3901 aagcataaca ggagagcatg gagtcggatt tgataagaaa agatttatgg acaagatgtt
     3961 ttccgaagac gaccttgata ccatgaatat gataagatgc acatttgacc ataaaaacct
     4021 gtgcaatccg ggaaaggtat ttcccacacc aagaacttgc gtagaacccg gaatgaaaaa
     4081 attcacccct catgagctgc acgaaaatgg tacagctgaa atgtactgat cccagacagg
     4141 taataatgga taaagcacta aaagacattg aaaacgtagt aggagctaaa tatctgtcca
     4201 caggagaaca ggctcttagc agctacagag ttgatctcaa gacaccggta gccgttatat
     4261 atccgggtac tgatctcgaa atatctgaaa taataaaaat ctgctctaca cataaactgt
     4321 cattaacccc atacggtaac ggcaccaaaa tcagcacagg aaatacccca aataaacttg
     4381 agctggtatt aagtacatcc aggctggata aaattatcga ccatggaagc gatgatctta
     4441 tagctactgc agaggctgga atcactctac aggaattcca agcagtacta aaaaagaaaa
     4501 accaacaggt agccgttcat ccacccaacc aaaaagaagg ctgcactctt ggcggtatga
     4561 ttaacacaaa cgattatggc ccatccagac taagatatgg ctcagtaaga gagaatttat
     4621 tagcgctcaa atttgtaagg gccgacggca agattgttaa aggcggggca aaggtagtta
     4681 agaatgtagc aggctatgat attcccaagc ttatgaccgg aagccttggt acacttggaa
     4741 ttattactga agccacattc aggctctacc cccagcagcc cgcatcaaac acaatcatag
     4801 caaatattaa taacatctcg caaatcaatg aaataaataa caaaatccta aactcc
//
LOCUS       DBNGSV010000331         5817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_130119, whole genome shotgun sequence.
ACCESSION   DBNGSV010000331 DBNGSV010000000
VERSION     DBNGSV010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_130119"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5800)
                     /locus_tag="AC159U_11760"
     CDS             complement(<1..5800)
                     /locus_tag="AC159U_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337770.1"
                     /translation="MYKKALIIKALIFEHLFEFDNSTDNLQKAIEHYTQALDCVSDQV
                     DSINVALIKNQIGVNYNILAETADKTENSKKSVKFHGEALTSISKETHLYEYSYIKMC
                     EGNSYRLLAEEEESQDNCLLAQRCYEASLGHFSADEYPREYAIVNNNIGSTFEFLAAI
                     TDDKNYLEKAIAAYEQTLKIYNIKDNPYEYAAVQNNLGNSFGARSEFEDPAENGSKAI
                     TAYTEALKVRTLSDYPKEYAMTQNNLGVVYDIISEHEDKEANSKSAIECYKESIKFYD
                     EESFAENYAMIQKNLGGAYRRLSEVSNAKDNSLLAKDAYEKALRFYTKDDFAEDYSLI
                     QKNISSLLTNVARYSKDKGELIKSIEQHNATLKELDQNTDSDEYAFVLNDICILKSAL
                     FDLEHTESNLKSLSESCNRALEVITPEDNSSEFASINKMLSDAFISYLETNSSEDYCL
                     DAIGFGKQALKVYKSKDYPADNAKLNENIGIAQSSLADFKDKDTNYKESVKSFKEALK
                     YYDANNHPIDNARINNNLGNVYKSLSEVEGTEKNLKNAIDSYDKALGAYTENDFPYEY
                     SKVKNNLAIAYSALGLLKNDSEILNKSIDALKKALSIRDKEQYPEEYAASCYNLGSTY
                     SKCYELSGETENLKQCIESYKDSLDLRKADKYPNEFAATSNSLGNAYRALSLVEDTAV
                     NLGLAKDCYTSCLEIYTEKDFPEQYAAVQNNLGVVYSSIGEINNDLSSYEKAIEHYGK
                     SLVIRDKKNYPKHYANINSGLGSNYFLLSQSKDTVDNLKKALECYTESMDYYTSKDFP
                     FDHYFLQHRLGNIHFELSEVENINENATKAQQYFINSLKYFSAKEYKAENTQINKNLG
                     EIYYLLAEEGDHKKNVASSIKHLEQAVNNITPEDDKDLYSSVQFLLGRSYEDYSNIEK
                     TEEYLQKARDSFSQAVELIDPENKPYEYSQISSTLGGIYTKLSAYGDKLANILKSIEL
                     YEQALSPNAIGRDDFEYGYISKDIGVSYTLLAKEEDKLENCKKAIESFNNALKVEDIK
                     NDPYQFGTVNKELASAYDTISQEEQKIENSRLAAEAYKKALQVFSYDDYPEDFGLINY
                     RLGRLLGTLSKAADKDSNLNEALQYLQSACEYYDEKSYPRQYADIQSEMGSCHRALAD
                     ISSEPQKTELINKSIDCLKNSLKIYDVKSHPADFASISNELGNNYLVLSDLHKDALSC
                     KNAIKAYEDSLNIRTSDNYPVQHALTSNNLGVAYISLSEIEDKIKNCEASIKAYSDSL
                     RVYTEDNYPLQFASINCNMGSAYSILAIEEQDVEQNLENSIKSYEQSLKIYNNDEFPH
                     EFATVQKGIADLYYQKYLLNQIEEDLTKAVQLYEQIVETKAFDNDSEEFAQLNKNLGS
                     TCLSLSEITKSDKSLLEKASGCFEIALKYYTQDDFENEYYEITKNIAEIGLKLYLESG
                     STELFDKASKQFENCIDYYNPTDFTEEHKNIQISLGRLLSSKYERKNDLDTLKLAIDH
                     FEAAADLINFKDSPSDFGFVNFELGKLHKYRAELEDNIDILNVSLIHINHALKFYHPN
                     EYKKEYEQLQDIISDIKPQLLELEEKDYGDSKEELTEALHYYNEMLQSLDKSKSEDRY
                     YSFKNKLATTFLSLYEKEDNKDHLQKALDNFKDVLENYSSITAEDKAILNTNIASAYY
                     LIGDMGKDKDTLEHAISHNKDALEYFVAGDYPFENSKIHDINGKIYLSLSESENFIDN
                     IISAIDSFDKSCVIDREEYPQEYKNIENNFNKLYDLLESKEDELTGEEEYCNKLITIY
                     EQLLNISFIKEYLPKYSETKKRIGHLYSVVADIGKEAENYDKSIDKYTEALEFFSAKD
                     NPQEYAYLNRSLAKAYSYSYIESAEQSKIDNAIDAYNKCIDYYEKNDYGREHAEVQTE
                     LGDLFVKLQEKSPGDENLMQALEYYKTAIEIYS"
BASE COUNT         1646 a         1075 c          953 g         2143 t
ORIGIN      
        1 ttgaatagat ttcaattgcg gttttataat attcaagggc ctgcatcagg ttttcatcac
       61 ccgggctttt ttcctgtaat tttacaaaca ggtcaccaag ctctgtctga acctctgcat
      121 gttcgcgccc atagtcgttt ttttcataat aatctatgca tttgttataa gcatctatgg
      181 cattatctat tttactttgt tctgcagact ctatatatga atagctataa gcctttgcaa
      241 gtgacctgtt taaataggca tattcctgag gattgtcctt tgcactaaaa aactctaaag
      301 cttcagtgta tttatctatg gatttatcgt agttttcagc ctcttttcct atatcagcaa
      361 ccactgagta caggtggcct atcctttttt tagtttcgga atatttcggt aaatattcct
      421 taatgaagct gatgttaaga agctgttcat aaatggtgat aagcttatta cagtattcct
      481 cttcacctgt aagttcgtct tcttttgatt caagcaggtc atacaattta ttaaagttat
      541 tttcaatgtt cttgtattcc tgtggatatt cttccctgtc aatcacacag gatttatcaa
      601 aactatcaat ggcgcttata atgttatcta taaaattctc agactcggat aagctcaaat
      661 atatctttcc gttaatatca tgaatctttg aattttcaaa tggataatcc ccagcaacaa
      721 aatattctag agcatcctta ttatgagaaa tagcgtgctc aagagtatct ttatccttac
      781 ccatatctcc aatgagataa tatgctgacg caatgtttgt attgagtatg gctttatctt
      841 cagcagtaat tgaactgtag ttttctaaaa catctttaaa attatccagg gctttttgaa
      901 gatggtcctt attatcttct ttttcataaa gtgaaagaaa tgtagttgcc agtttatttt
      961 taaaagagta ataacggtct tcagatttag atttatcaag ggactgcaac atttcattgt
     1021 aataatgcag agcttctgtc aattcttctt ttgaatcccc atagtctttt tcttcaagct
     1081 caaggagctg gggcttaata tctgagatga tatcctgcaa ctgctcatat tcttttttat
     1141 attcattagg atgataaaac ttgagggcat ggttaatgtg tatcagtgaa acatttagaa
     1201 tatcaatgtt atcttctagt tctgccctgt atttatgaag ttttccaagc tcaaaattaa
     1261 caaaaccaaa gtcgcttgga ctatccttaa aatttataag atccgctgct gcttcaaaat
     1321 gatctatggc aagttttagc gtatcaagat cgtttttcct ttcatatttt gaacttaaaa
     1381 gccttccaag acttatctga atatttttgt gttcttctgt aaaatcagtt ggattataat
     1441 aatcgatgca attctcaaat tgtttgcttg ccttgtcaaa caactcagta ctgccagatt
     1501 ctaaatacag ttttaatcca atctcggcga tgttcttcgt aatttcatag tattcattct
     1561 caaagtcatc ctgagtataa tactttagtg caatttcaaa acacccggaa gccttctcaa
     1621 gtaaagattt atcactctta gtgatttcgg atagggataa acaagtgctg ccaagatttt
     1681 tgtttagctg agcaaattct tcagaatcat tatcaaaagc tttagtttca acgatttgct
     1741 catacagctg cacagccttg gttaaatctt cttcaatctg gttcagcaga tacttctggt
     1801 aatataaatc tgctatgcct ttttgtacag ttgcaaattc atgagggaac tcatcattat
     1861 tataaatttt gaggctttgc tcgtaggact ttatagagtt ttccaggttt tgctctacat
     1921 cctgttcctc aattgcaagt atactgtacg ccgagcccat gttgcaatta attgaagcaa
     1981 actgaagtgg ataattatct tctgtataaa ctcttaaact gtcactatat gccttaattg
     2041 aagcctcaca atttttaatc ttatcttcaa tttctgataa actgatatag gctaccccaa
     2101 ggttgttact tgtaagtgca tgctgtacgg ggtagttatc agaagttctg atattaagtg
     2161 agtcctcata tgccttgatg gcgtttttgc agcttagggc atctttatgc aaatcagaca
     2221 ataccaaata attgtttcca agttcgttgc ttatacttgc aaaatctgca ggatgagatt
     2281 taacgtcata tatttttaaa ctgtttttaa gacaatctat agatttattt ataagctcag
     2341 ttttttgagg ctcagaactg atatcagcaa gagccctatg acatgaaccc atttcagact
     2401 gaatatctgc atattgccga ggatatgatt tctcatcata atattcacaa gcgctctgta
     2461 aatattgcaa agcttcattt aggtttgaat ctttatctgc agctttggaa agtgttccaa
     2521 gaagtcttcc aagtctataa ttaattaaac cgaaatcttc ggggtagtca tcataactaa
     2581 atacttgcaa tgcctttttg taggcctctg ctgcaagtct cgagttttca attttttgtt
     2641 cctcttgaga aatagtatcg tatgcagacg caagctcttt atttactgta ccaaactgat
     2701 atggatcatt ttttatatct tcaactttta gagcattgtt aaatgattct attgcttttt
     2761 tgcagttttc aagtttgtct tcttcttttg caagaagtgt atagcttacg cctatatcct
     2821 tagaaatata gccatattca aagtcatctc tgccaatagc atttggtgag agggcctgtt
     2881 catatagctc aatggatttt aaaatgtttg caagtttatc tccgtatgca gaaagtttgg
     2941 tataaatacc accaagcgtt gagcttattt ggctatactc atatggctta ttttccgggt
     3001 caatcagttc caccgcctga ctgaatgaat cccttgcctt ttgtaaatat tcctcagttt
     3061 tttctatatt ggagtagtct tcatatgacc tgcccaacaa aaactgtaca gatgaataaa
     3121 gatctttatc atcttctggg gtgatgttat taacagcctg ctctaagtgt ttaattgagc
     3181 ttgcaacatt ttttttatga tcaccctctt cagccaagag atagtaaatc tctccaagat
     3241 tcttgtttat ctgagtattt tcagccttat attccttggc actgaagtac ttgagtgaat
     3301 ttatgaagta ttgctgagct tttgtcgcat tttcgttgat gttttcaact tcagatagtt
     3361 caaagtgtat atttcccaat ctatgctgca ggaaataatg atcaaatgga aagtccttgg
     3421 atgtgtaata atccattgac tctgtataac actcaagagc cttttttaag ttgtccactg
     3481 tgtctttact ttgagacaga agaaagtagt ttgacccaag cccggaattt atatttgcat
     3541 aatgcttggg ataatttttc ttatccctta ttacaaggga cttaccgtag tgttcgattg
     3601 ctttttcata gcttgaaaga tcattattta tctccccgat tgatgaatag accactccca
     3661 aattattttg tacagcggca tattgttctg gaaaatcttt ttcagtataa atttcaaggc
     3721 agcttgtata acaatctttt gcaaggccaa ggttaacggc agtatcttct acaagtgaaa
     3781 gcgccctgta agcatttcca aggctatttg atgttgcagc aaactcgttt ggatatttat
     3841 ccgcttttct aaggtcaaga gaatccttat atgattctat acattgcttt aaattttcag
     3901 tttctccgct taattcataa cacttgctat atgtagaccc caaattgtag cagcttgcgg
     3961 cgtattcttc gggatattgt tccttatctc taatactaag agctttttta agtgcatcaa
     4021 tagatttatt aagaatttct gaatcatttt ttagcagacc aagagcagaa tatgcaatgg
     4081 ctaaattatt tttaactttg ctgtattcat atggaaaatc attttcagta taagcgccaa
     4141 gagctttatc atatgaatca atcgcattct taaggttttt ttcagtacct tcaacctcag
     4201 ataaagattt ataaacattt ccaagattat tattaatcct tgcattatct atagggtgat
     4261 tatttgcatc ataatacttc agtgcttctt taaatgattt aaccgattct ttataatttg
     4321 tatctttgtc cttaaaatca gcaaggctgc tttgggctat accaatattt tcatttagtt
     4381 ttgcattatc tgcgggataa tcttttgatt tataaacctt tagcgcctgc tttccaaaac
     4441 caatggcatc aaggcagtaa tcttcactac tatttgtttc gagataactt ataaatgcat
     4501 cagaaagcat tttgtttata gatgcaaatt cagagctatt atcttcaggt gtaataacct
     4561 caagcgcacg attgcagctt tctgataaac tttttaggtt tgattcagta tgctccagat
     4621 caaaaagtgc tgattttagg atacaaatat catttaaaac aaatgcatat tcatctgaat
     4681 ctgtattttg atcaagctct tttagagtag cattgtgctg ttcaattgat tttatcaact
     4741 cgcctttatc ttttgaatat cttgctacat ttgtgagaag agacgatatg tttttttgaa
     4801 ttaagctgta gtcttcagca aaatcatcct tggtgtagaa tctaagagct ttttcgtatg
     4861 cgtcttttgc aagcaggctg ttatcttttg cattgcttac ctcagatagc cttctgtatg
     4921 ctcccccaag gttcttttgg atcatcgcat agttttctgc aaatgactct tcatcataaa
     4981 acttaatcga ttccttataa cactctatag cacttttact gttagcctct ttatcttcat
     5041 gctctgaaat aatgtcataa acaacaccga gattgttctg ggtcattgca tactctttgg
     5101 gatagtcact aagagttctt accttaagcg cctctgtgta tgcagttatt gcttttgagc
     5161 cattttccgc aggatcttca aactcagaac gagccccgaa agaattacca aggttatttt
     5221 gaactgctgc atattcatag ggattatcct taatattata aatttttaag gtctgttcat
     5281 aggcagcaat tgctttttct agataatttt tatcatctgt aatagctgca agaaattcaa
     5341 atgtggaacc tatgttgttg tttacaattg catactccct tggatactcg tcggcagaaa
     5401 aatgtccaag agatgcctca taacaccgct gcgcaagcag acagttatcc tgtgattctt
     5461 cttcctctgc caaaagcctg taggagtttc cctcacacat tttaatatag gaatattcat
     5521 ataagtgggt ctctttggaa atagaagtta atgcttcacc atgaaacttg actgatttct
     5581 tggagttttc agtcttgtct gctgtctctg ccaggatgtt gtaattcaca ccaatttgat
     5641 tctttattag cgccacattt attgaatcaa cctgatcact tacacagtca agagcctgag
     5701 tatagtgctc aatagccttt tgcaggttgt cggtactatt atcaaattca aaaagatgct
     5761 caaagatcaa agctttaata ataagggctt ttttatacaa tcttacatcc gtatttt
//
LOCUS       DBNGSV010000332         4795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1030927, whole genome shotgun sequence.
ACCESSION   DBNGSV010000332 DBNGSV010000000
VERSION     DBNGSV010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1030927"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1065)
                     /locus_tag="AC159U_11765"
     CDS             complement(55..1065)
                     /locus_tag="AC159U_11765"
                     /inference="COORDINATES: protein motif:HMM:NF020455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HIR3337771.1"
                     /translation="MIFEWKRLKFTFITLPCLLIAFLAICVFCTEANAQGNYDDGLAF
                     ILVQAKLPRGAERNTNRPGKDYKNFNLEEPNPKLCMAACEKDAKCRAYTYVKPGVQGK
                     KARCWLKSGVPKPKKNKCCISGVKTIQETVVEKSTEVPDDNIPPGSYQQTCTDISVSG
                     TTLTAYCARISDTDMMYRSDLADINLCRGDISNNDGRLTCKKPTNIPRGSYMQTCENI
                     SVSETTLTAYCATKSDSRMTHRTQLSEIDLCLGDISNNDGYLTCEKTYYPPGSYQQTC
                     RVISVSGTTLTAYCAKKSGDWGMIYRTQLSEIDLCRGCRCHVIRGQFFRNSTGGTPPR
                     DW"
     gene            complement(1096..2166)
                     /locus_tag="AC159U_11770"
     CDS             complement(1096..2166)
                     /locus_tag="AC159U_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337772.1"
                     /translation="MSGTTLSAKCKTKSGWFRQKSTIEEFDRCTGDISNFDGYLTCNK
                     SGELPPGSYLMSCTNPSLSGTGLTAICDNKKGHTITSTLEQVDHCTGDISNNDGYLTC
                     EKSNAPAGSYLESCVNINISGTTLSAFCTTKKSRYGSYSYRKSTLEELDRCIGDISNL
                     EAKLTCQKSGNVPSGSYMRTCTNPSLSGNTLSAVCEYVMSSSGDRRTKETTLEQTDLC
                     VGDISNHDGYLTCEKSNAPPGSYLQSCVNINVSTTTLTARCRKETSYGHTWPKPTLRE
                     FDRCTGDISNFHGILTCQKSGNVPQGSYLQSCMHPSLSGTTLTAICETTSDTSKFSTL
                     NDFDRCTGDISNNNGNLTCQKN"
     gene            complement(2213..2647)
                     /locus_tag="AC159U_11775"
     CDS             complement(2213..2647)
                     /locus_tag="AC159U_11775"
                     /inference="COORDINATES: protein motif:HMM:NF025653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HIR3337773.1"
                     /translation="MSKINQPLNAKAFCVNICYLSVICLLVIVFGKSVQAHTQPNSEI
                     EPAHSLIVAKLPEGAEPNTNRPGKDYKNFDLEAPKPKLCMAACEKDPKCRAYTYVKPG
                     VQGEKARCWLKSAVPEPKKNKCCISGVKKNQRTQYRKKHRST"
     gene            complement(2972..3472)
                     /locus_tag="AC159U_11780"
     CDS             complement(2972..3472)
                     /locus_tag="AC159U_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3337774.1"
                     /translation="MPIYEFYCKKCNTMYQFFSRTINTETIPKCPSCKKPKLERMMSG
                     FATISESSDESESSNDIPDIDDATVQKAMSLIEKNLLNVDVDNPDPRQTTQLMKDLSS
                     MSGLEMGEGMQEALSRLEKGEDPEKIEQEMGNILKDEDQFFKKTSRAKKRSSRPRKDE
                     TLYDLE"
     gene            3592..3987
                     /locus_tag="AC159U_11785"
     CDS             3592..3987
                     /locus_tag="AC159U_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR3337775.1"
                     /translation="MKCPVCKVQLIVVERHKIELDYCISCKGFWFDRGELNLLSETLG
                     LDLIKELQTTQLNEYSEKPRDCPLCDNRMDKIKLGQNTNVVIDKCPQGQGMWFDGGEL
                     SRIVNQNREISGLGNKEMINFLGEFLVQG"
     gene            4049..4609
                     /locus_tag="AC159U_11790"
     CDS             4049..4609
                     /locus_tag="AC159U_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3337776.1"
                     /translation="MSSLLILLGIAAVVVVVLASMYNGLVNAKNSVKNAWSQIDVQLK
                     RRHDLIPNLVETVKGYVKHERETLEAVTKARQQAIDLSGASVAERAKIENFLTQTLKS
                     LFAVSEAYPDLKANENFLNLQEEITTTENRVSFSRQAYNDEVLNLNNKVEMFPTNIIA
                     GMFGFSQGEYFEIDDLAEKEVPKVQF"
BASE COUNT         1380 a          912 c          941 g         1562 t
ORIGIN      
        1 ggaattaaaa caggaactgc ttgagtacct ggtgtattac aatcaccaaa gacctcacca
       61 gtctctgggg ggagtaccac cagtagagtt cctaaaaaac tgtcctcgaa ttacgtgaca
      121 tctacaacca cggcagagat caatttcgga aagctgagtc ctatagatca taccccaatc
      181 accacttttt tttgcgcaat atgcagtcag agttgttccg gacacactaa tgactctaca
      241 cgtttgctgg taagagcctg gtggatagta tgttttttca caggtcagat aaccgtcatt
      301 gttgcttata tccccgagac agagatcaat ttcggaaagc tgagtcctgt gtgtcatacg
      361 ggagtcactt tttgttgcgc aatatgcagt cagagtcgtt tcggacacac taatattttc
      421 acaggtttgc atgtaggagc cccgtggaat gtttgttggt tttttacagg tcaatctacc
      481 atcattgttg ctaatatccc ctcgacagag attgatgtcg gcaagatcag acctgtacat
      541 catgtcagtg tcgcttattc ttgcgcaata tgcagtcaga gtcgttcctg atacactaat
      601 gtctgtgcac gtttgttggt aagagcccgg tggaatatta tcatccggca cttccgtact
      661 tttttccaca acagtttctt gaatggtttt aacgccggaa atacagcatt tatttttctt
      721 tggtttaggc acgcctgact taagccagca gcgggccttt ttcccctgta cccctggctt
      781 aacatatgta tatgccctgc actttgcatc cttctcacat gctgccatac aaagcttagg
      841 gtttggttct tccaggttga agtttttata atccttcccg ggcctgtttg tatttcgctc
      901 cgcgcccctg ggcagttttg cctgtaccag tataaacgcg agtccatcat cataattacc
      961 ctgagcgttt gcttctgtac aaaagacaca tattgctaag aacgcaatta atagacatgg
     1021 aagtgtaata aaagtaaatt tcagtctttt ccattcaaat atcatgatgt ctctcccccc
     1081 tcttttataa agccgtcaat tcttttgaca ggtcaggttg ccgttgttgt tgctaatatc
     1141 gcctgtacag cggtcaaaat cattgagagt tgaaaatttt gaagtatcac ttgtagtttc
     1201 acatatagca gtaagtgtag tgccggataa gctggggtgc atgcaggact gaaggtaaga
     1261 gccctgaggc acattgccgc ttttctggca tgtcaaaatc ccatgaaagt tacttatgtc
     1321 acctgtgcag cggtcgaact ccctaagagt tggtttgggc catgtgtgtc catatgacgt
     1381 ttctttcctg caacgagcag ttaaagtggt tgtggatacg tttatattca cgcaggattg
     1441 aagatacgaa cccggtggcg cgtttgattt ttcgcaggtc aggtaaccat cgtggttgct
     1501 tatgtcccca acacataggt cagtttgttc gagagtcgtt tctttcgttc gtctatcacc
     1561 tgacgatgac atgacatatt cacatacagc actaagtgta ttgcctgata agcttggatt
     1621 tgtgcaggtt ctcatgtatg aaccggaagg cacattacca cttttctggc atgtcaattt
     1681 agcttccaaa ttacttatat cacctatgca gcggtctaat tcctcaagag tagattttct
     1741 gtaactataa gatccatagc gacttttttt tgttgtacag aaagcagata aagtggttcc
     1801 ggatatgttt atattcacac aagactcgag gtaagatcca gcaggtgcat ttgatttttc
     1861 acaagtcagg taaccatcat tgttgcttat atctcctgta caatgatcaa cttgctcgag
     1921 agttgatgta atcgtatgac cttttttgtt atcacaaatt gcggtcaagc ctgtgcctga
     1981 taggcttgga ttagtgcagg acattaagta agagccggga ggtaattctc cgcttttgtt
     2041 acaagtcaag taaccatcaa agttactaat atcgcctgtg catcgatcaa attcctcgat
     2101 tgtagatttt tgtctaaacc agccactttt tgttttacat ttagcgctta aagttgtccc
     2161 ggacacctta atacctttac atgtttgctg gtaagagccc ttaggaatag catcatgtac
     2221 ttctgtgctt ttttctatac tgggttcttt ggtttttttt cacccctgaa atacagcatt
     2281 tattcttttt tggctcgggc acggctgact taagccagca gcgggccttt tcaccctgca
     2341 caccgggctt gacatacgta tatgctctgc actttggatc cttttcgcat gccgccatac
     2401 aaagcttcgg ttttggcgct tcaaggtcga agtttttata atccttcccg ggcctgtttg
     2461 tgtttggctc tgcgccctcg ggaagttttg ctactatcag gctgtgcgca ggttcaatct
     2521 cagagttagg ctgagtatgc gcttgcacac ttttcccaaa tactattacg agcaaacaga
     2581 ttacagacag atagcagatg tttacgcaaa aagcttttgc atttagtggt tggttaattt
     2641 tagacatttg tcccccctgt ttattcaata aattgtttga actaaataaa ctcaaagtct
     2701 cataattaac tgttgtgcaa gccttatacc actaattgat atctgtataa cctattgtaa
     2761 acattggcaa atatataaat aattcgactt tcagaagaga ttatattcgt ctagaattca
     2821 tacatctgac aataaggatt gaataaatta ttactacttc ggaaataagt tattgaaggg
     2881 gtttatctat ttaatcaata agtatttttc attgagaatt attggcaagc gaataaaaca
     2941 aattctgttt attgattagc tacgcgttaa atcactccaa atcatataga gtttcatctt
     3001 tccttggtct ggaacttctt ttttttgctc ttgaagtttt tttaaaaaac tgatcttcat
     3061 cttttaaaat gttacccatt tcctgttcta tcttttcagg atcttcacct ttttccagtc
     3121 tactaagggc ctcttgcata ccttctccca tctcaagtcc tgacatactc gataagtctt
     3181 tcataagctg tgttgtctgc cgtgggtcag ggttatcaac atcaacattt aataaattct
     3241 tttcaatcaa agacattgct ttttgcacag tagcatcgtc tatatcaggt atatcattag
     3301 aagattctga ttcatcgctg ctttctgaaa tagtggcaaa ccccgacatc attctctcaa
     3361 gctttggttt tttgcatgaa ggacatttgg ggatcgtttc ggtatttatt gtcctcgaaa
     3421 aaaactgata catggtgttg catttcttgc agtaaaattc ataaatgggc actgtgaact
     3481 cctccaatca ttctaaaata aataatttaa ctgttggttt tgttttatat atcaattatt
     3541 atatcaacat tatatatagc aattcaataa tgaacattaa attataattt catgaaatgt
     3601 ccagtttgca aagtacaatt aatagttgtc gaaaggcaca agatagagct tgattactgt
     3661 atttcatgta agggattctg gtttgataga ggagagctca atcttctatc tgaaacacta
     3721 gggcttgatc tgataaaaga gttacaaact acacagttaa atgaatatag tgaaaaacct
     3781 agagactgtc cactatgtga taatagaatg gataagataa aactcggaca aaatactaat
     3841 gtagttatag ataaatgtcc gcagggacaa ggtatgtggt ttgatggtgg agagcttagc
     3901 agaattgtta atcagaacag agaaatatct ggtttaggaa ataaagaaat gattaatttt
     3961 ttaggagaat ttctagtaca gggctgaaag aaaataactt aacttgaaat taccaaaaaa
     4021 tgattatatt aaacgttagg aggttaacat gagcagttta ttaattttac ttggaatagc
     4081 agcagtagta gtggttgtat tggcatcaat gtacaacggg cttgtaaatg caaaaaacag
     4141 tgtaaagaat gcatggtcac agatagatgt acagctaaag cggaggcatg acctgatacc
     4201 aaacctggta gagacagtaa agggttatgt taagcatgaa agagaaactc tagaagcagt
     4261 aacaaaagcc agacagcagg caatcgattt atcgggtgcg agtgttgcag aaagggcaaa
     4321 gattgaaaat tttcttacac aaaccctaaa atctctcttt gcagtcagtg aagcatatcc
     4381 tgacctaaag gccaatgaga attttcttaa ccttcaggaa gagatcacga caactgaaaa
     4441 cagggttagt ttttccagac aggcttataa tgatgaggta cttaatttaa ataataaagt
     4501 agaaatgttt cccacaaata ttattgcagg aatgtttgga ttttcacaag gagaatattt
     4561 tgaaattgat gatcttgccg aaaaagaagt gcccaaggta caattttaat ttgttgaacc
     4621 caacgtagat tgcaaattgt acttttgtat tgattatagc ttataaattt agggcattag
     4681 acatctggcg gagtagaatt cattaaaata ttaattattt tattcatcat ttcgtcagta
     4741 ctaagatgtc tgttattaaa cctaaactcc tgttccttta cataatatat cagat
//
LOCUS       DBNGSV010000333         1790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1932134, whole genome shotgun sequence.
ACCESSION   DBNGSV010000333 DBNGSV010000000
VERSION     DBNGSV010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1932134"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1685
                     /gene="groL"
                     /locus_tag="AC159U_11795"
     CDS             27..1685
                     /gene="groL"
                     /locus_tag="AC159U_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098521.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3337777.1"
                     /translation="MGAKEIKFNREAQDLILQGVNELANAVKSTLGPRGRNVLIEKSF
                     GAPVVTKDGVTVAKEIELENKFQNMGAQMVKEVASRTSDVAGDGTTTATVLAQSIYKE
                     GIKLVAAGHDPMKLKRGIDKAVETVVDSIQKLSKQVKGRTEIAQVATISANGDENIGN
                     IIADAMEKVGKDGVITVEEGRSLDTALDVVEGMQFDRGYLSPYFVTDAEKMIVDLDEP
                     LILLHDKKIASMKDLVPLLEESARTSKPLLIIAEDIEGEALATLVVNKIRGTLKVAAI
                     KAPGFGDRRKAMLEDIGVLTGGKVIAEEAGMKLENTTVADLGRAKKVVIDKDDTTIVS
                     GEGKKGDIEGRIRQIKAQIDESTGEYDREKLQERLAKLAGGVAVIRVGAATEAEMKEK
                     KARVEDALNATRAAVEEGVVPGGGVALVRAIKTADSFDTGDTEEQAGVNIIKRVLEEP
                     LREIAGNAGWDGSIVVEKVKESKGSNGFDAAKLEYCDMLAAGIVDPAKVTRSAIQNAA
                     SVAGLLLTTAALVAEKPAPEEPIGAPGGAGPMGGMHPGAMGGMM"
BASE COUNT          652 a          290 c          512 g          336 t
ORIGIN      
        1 aaaaaaatta ggaggcaata atcaacatgg gagcaaaaga gataaaattt aacagagaag
       61 cccaggacct gatactgcaa ggtgtaaatg aacttgcaaa cgcagtaaaa tccacccttg
      121 ggccaagagg cagaaacgtt ctgatagaga aatcattcgg agcacctgtg gtaacaaagg
      181 acggagtaac agtagcaaaa gagatagagc ttgagaacaa attccagaac atgggcgcac
      241 agatggtaaa ggaagtagca agcagaacaa gtgacgttgc aggagacgga accacaacag
      301 caacagtgct tgcacagtcc atatacaaag aaggaattaa acttgtagca gcaggccacg
      361 acccaatgaa actaaaaagg ggtatagata aagcagtaga aacagtagtg gactctatac
      421 agaaactaag caaacaggta aagggcagaa cagagatagc acaggttgct acaatctcgg
      481 caaacggtga tgagaacata ggaaacatca tagccgatgc aatggaaaaa gtaggaaagg
      541 acggagtaat cacagtagaa gaaggcagaa gccttgatac cgcacttgat gtggtagagg
      601 gtatgcagtt tgatagaggt tatttaagcc catacttcgt aacagatgca gagaagatga
      661 tagtggactt ggatgagccg ctgatacttc ttcatgacaa gaagatagca agcatgaagg
      721 atctggtacc actgctcgaa gaatcagcaa gaacatcaaa gccgctgctt attatagcag
      781 aggatattga gggtgaggct cttgctacat tagttgttaa caagataaga ggaacactaa
      841 aggtagcagc aataaaggcg ccagggtttg gagacagaag aaaggcaatg ctagaggata
      901 taggggtgct tacgggtgga aaggtaatag cagaagaagc gggcatgaaa cttgaaaaca
      961 ccacggtggc cgatcttgga agagcaaaga aggtagttat agacaaagat gatacaacaa
     1021 tcgtaagcgg agaaggaaag aagggtgaca tagagggaag aataagacag ataaaggctc
     1081 agatagatga atccaccgga gagtacgata gggaaaagct acaggaaaga ctagctaagc
     1141 ttgcaggtgg agtagcggtg ataagagtag gagctgcaac agaagctgag atgaaagaga
     1201 agaaggcaag agtagaggat gcgcttaatg caacaagggc agctgtagaa gagggggtag
     1261 tccccggggg aggagtagca cttgtaaggg caattaagac agcagattct tttgatacag
     1321 gagatactga agagcaggca ggagttaata tcataaaaag agtactggaa gagccactta
     1381 gagagatagc aggcaatgca ggctgggatg gttcaatagt ggtagagaag gtaaaggaat
     1441 ccaagggatc aaacgggttt gatgccgcaa agctagagta ctgtgatatg cttgcagctg
     1501 gtattgtgga tcctgcaaag gtaacaagat cagcaataca gaatgcagca agtgtggcag
     1561 ggctgctgct tacaactgca gcgcttgtag cagagaagcc ggcacctgag gagccaatag
     1621 gagcgccagg tggtgcagga cctatgggtg gaatgcatcc cggagcaatg ggcggcatga
     1681 tgtagtctct atgagacaga tataacactt gttaaacaag taagataatt tatttaaaat
     1741 caaacagggc gggttactat ttatttaagt agcccgcctt tttttatcta
//
LOCUS       DBNGSV010000334         1828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1308520, whole genome shotgun sequence.
ACCESSION   DBNGSV010000334 DBNGSV010000000
VERSION     DBNGSV010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1308520"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..941
                     /gene="smpB"
                     /locus_tag="AC159U_11800"
     CDS             486..941
                     /gene="smpB"
                     /locus_tag="AC159U_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3337778.1"
                     /translation="MQKTVCTNRTALRDYHIEEKIEAGIELKGSEVKSLRLSQASLKE
                     SFGIVRDGQIYLVNSYIAPYVEANNFNHEPKRERKLLLHRKEINKLIGKSQSKGYTLV
                     PLKIYFKNKFAKLELGLGKGKKNIDKREDIKRKDAQREMERAIKNKRYN"
     gene            1011..1367
                     /gene="ssrA"
                     /locus_tag="AC159U_11805"
     tmRNA           1011..1367
                     /gene="ssrA"
                     /locus_tag="AC159U_11805"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1420..>1828
                     /gene="rph"
                     /locus_tag="AC159U_11810"
     CDS             1420..>1828
                     /gene="rph"
                     /locus_tag="AC159U_11810"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR3337779.1"
                     /translation="MRSDGRKSDELRPIFIETDVMKYAEGSAIIKAGNTHVVCSATVE
                     DSVPHFLRDTGSGWVTAEYSMLPRSTHTRNKRDAAKGRINARAQEIQRLIGRALRSVV
                     DLDKLGEQSIILDCDVMQADGGTRTASITAAFVA"
BASE COUNT          651 a          272 c          369 g          536 t
ORIGIN      
        1 taaagcgaat gctatctgtt gttctgtaaa tcttgacttc ctcatggcaa aatacctcct
       61 gatttattgc attattttgc cagatttctc tacttttacc tggttcagtt tgcagggtga
      121 aggtcaataa gacatcatta tattttatta aaactagttt attatcaaac atattgatta
      181 ataaattatt agtctaatat tatataaata agaaactatt caaagtgttg ataaaacata
      241 tgtatctaat gtaattaatt aactttggag ggaaagttct aatttagaga attataaata
      301 ataataaacc ttcccaaccc cgggatgaaa tttatttact ctaaattaga acttaggcta
      361 taataatata tgataaatag ttaaagttca atattaggta aatataatca catgattaca
      421 ggatgttaca tatatatctt aaagtaaata atagtaaaat gttgataagt aaattataaa
      481 aaattatgca aaaaacagta tgtacaaata gaactgctct tagagattat catatagaag
      541 agaaaattga ggctggaatt gagctaaaag gttctgaggt taaatctctg aggcttagtc
      601 aggcaagtct taaggaaagt tttggaatag tacgggatgg gcagatttat cttgtaaaca
      661 gctatatagc tccttatgtt gaagcaaata actttaacca cgaacctaaa agagaaagaa
      721 aactattgtt gcatagaaaa gaaattaata agcttattgg aaaatcacaa agtaagggat
      781 atacgcttgt tccccttaaa atatatttta aaaataagtt tgcaaaatta gaactgggtc
      841 ttggtaaggg aaagaaaaac atagataaac gagaagatat aaagagaaaa gatgcccaga
      901 gggagatgga aagggcgatc aaaaataaaa ggtacaacta aatgatataa gatattgaaa
      961 caatatgata tgtgattata ttataaacaa gcgttaatat taaattatat gggggcgttt
     1021 tggcttcgac ggggaatggg atagcttgtg ctgcatgccg agcttcttct gctaactcgt
     1081 taaactggta gtaaaactat aagtgccgaa ccaatcaacg cactcgcagc ataaatgact
     1141 gcgacgtctc ttaaaccgcg cctgtcgggt ttaattcaga cgtcataaag acgggctggt
     1201 ctttaaggtt ttcttgtggc tggaaaagac gagatttctc gcaagatagt ggtgaaaata
     1261 ctcgcctttc ggtttatttc accgtgaaac tgaaagaagg gataagcatg tagaggcctt
     1321 gtgaggctct tcttcggacc tgggttcgat tcccagcgcc tccaccataa tttaatagta
     1381 attgttaata agtgtatctt ataaggagta aactatctaa tgcgatcaga cggcaggaaa
     1441 tcagatgaat tgagacctat atttattgaa acagatgtga tgaaatacgc agagggctct
     1501 gctatcataa aagctggaaa cactcatgtt gtctgttcgg caaccgtaga agactcagtc
     1561 ccgcattttt taagagatac tggttctggg tgggtaacag ccgagtactc tatgcttcca
     1621 agatctactc atacaagaaa taaaagagat gcagctaaag gaagaataaa cgcaagagcg
     1681 caggaaattc aaagactaat tggaagagcg ctcaggtctg ttgttgattt agataaactt
     1741 ggcgaacaat ctataattct tgactgtgat gtgatgcagg ctgatggagg cacacgcaca
     1801 gcatctatta ccgcagcttt tgttgcgc
//
LOCUS       DBNGSV010000335         4402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_269391, whole genome shotgun sequence.
ACCESSION   DBNGSV010000335 DBNGSV010000000
VERSION     DBNGSV010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_269391"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="serS"
                     /locus_tag="AC159U_11815"
     CDS             complement(<1..1214)
                     /gene="serS"
                     /locus_tag="AC159U_11815"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3337780.1"
                     /translation="MLDIKFIEDNFDLVITKTEQRNTKVDFDTLKELIDKRKTAIKEA
                     EQVEHKRNKGSKEVGSLMREGKKDEAKQLQADLKELSDTVKELNDKRTIIETDLRNIL
                     LQIPNITNEAVPVGKDESDNPVVKTWGEPTKFDYEIKDHVEIGKNLGILDLETAAKIT
                     GARFSLYKGLGARLERALINFMLDIHTKEHGYTEVLPPLMANSESFTGTGNLPKFEDD
                     LFKINDTDYYLIPTAEVPVTNIYRDEIISEEQLPIKYAAYTPCFRKEAGSYGKDVHGI
                     MRQHQFNKVELVKFTTPETSYDEHEALTANAAKILELLGLPYRIVVLCTGDIGFSSAK
                     TYDLEVWIPSENKYREISSCSNFEDFQARRAAIRYKPSGGGKTKLVHTLNGSGLAVGR
                     TVIAIMENFQNK"
     gene            complement(1219..2670)
                     /locus_tag="AC159U_11820"
     CDS             complement(1219..2670)
                     /locus_tag="AC159U_11820"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3337781.1"
                     /translation="MEHSRPIKTIKINDYLFEIPKTGRMLVPGRLYVNEKILKSIIED
                     RSIEQVVNVAHLPGIQKYSIAMPDIHLGYGFPIGGVAATDIETGVISPGGVGYDINCG
                     VRLMRTNLTEKDVRPSLRLLVEQLFKTVPCGPGIGGKIKLSDSDYNDFTDKGVKWAIE
                     RGWGIEQDLEMIEDRGYFSGSNLENISQKALERGRNQLGSLGSGNHFLEINVVDKIDN
                     LKVADAFGLSIGQVCVLIHCGSRGFGHQICSDYINKMLRYMEREQIKLPDKQLACAHI
                     NSKEGKEYLSAFAAAVNYAWINRQIIMHLTREAFMNVLNLSSADLGAALVYDVSHNIA
                     KIEEHKINGEMKKVCVHRKGATRSFPKNHPLIPDIYKDVGQPVMIPGDMGRASYVLVG
                     TENALEQTFGTCCHGAGRLLSRRRALKQGDPNKLLAELSKKGIEVIARKKRTIVEEMP
                     DAYKKVEDVCDVVHNAGIAKKVARLRPIGVIKG"
     gene            complement(2675..3094)
                     /locus_tag="AC159U_11825"
     CDS             complement(2675..3094)
                     /locus_tag="AC159U_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR3337782.1"
                     /translation="MNKSYEIIDHTADISVRVSGRDFNELLINSSLAMMDIICDLNTI
                     EAKNKYAVKSDGNSEEQLLVNWLSELLYMHEVKRLLFSDFEIKVNENKEVEGICKGEE
                     IDFSRHDLLNDIKAVTYSGLNVEHNNGLLSATITFDI"
     gene            3187..3906
                     /locus_tag="AC159U_11830"
     CDS             3187..3906
                     /locus_tag="AC159U_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3337783.1"
                     /translation="MANIGKGFEITYYGHSTFRIKSPKGKIIFIDPWITENPSCPENL
                     KDVDGVDIIAVTHGHFDHISDVRPLAEKYKPTVVSNWEICDWLGSKKVENCAPMNKGG
                     GQVVDGIKFFMIHAQHSSGIKDDDGSTIYGGEPAGFVIQLENGFRLYHAGDTNIFSDM
                     KLISEIYKPELVMLPIGDLFTMSPLEASYACRFLNPKYVIPMHYGTFPPLIGTPDQLK
                     ALTRDLTEMEIIALKPGEVLS"
     gene            complement(3903..4328)
                     /locus_tag="AC159U_11835"
     CDS             complement(3903..4328)
                     /locus_tag="AC159U_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3337784.1"
                     /translation="MARINIELPNDFIYCTEIKLRISDINYGGHLAHDSILSLTHEAR
                     VRFLQSLGYSEANIDGPGIILSDAALVYKSEAFYGQSLKIEIAVNEFSKYGCDFVYKL
                     SDSETLKEVARVKTGIVFYDYNKREISRVPQKFKSEFNS"
BASE COUNT         1158 a          920 c          788 g         1536 t
ORIGIN      
        1 tctttattct gaaagttttc catgattgct ataacagttc ttcctactgc cagacctgac
       61 ccgttcagag tgtgaacaag ttttgtcttg ccgcctccgg atggtttata tctaatggct
      121 gctcttcttg cctgaaagtc ttcaaagttg gagcaggatg agatttccct gtacttgttc
      181 tcgcttggaa tccagacttc aaggtcataa gtctttgcag aggaaaaacc tatgtcgcct
      241 gtgcatagga caactattct gtaaggaagt cctaatagct ccagtatctt tgcagcgttt
      301 gcagtaagcg cctcatgctc gtcataggat gtctcagggg tagtaaactt tacaagctca
      361 accttgttga actggtgctg tctcataatg ccatgaacat cctttccata agacccggct
      421 tcctttctaa aacaaggcgt ataagcagca tatttaatcg gaagctgctc ttcagatatg
      481 atttcatctc tgtaaatatt agttactggt acttctgctg tgggtatgag gtaataatca
      541 gtgtcattta ttttaaagag gtcatcttca aacttgggta gattgccggt gcctgtaaaa
      601 ctttcagagt ttgccataag aggtggaagt acctctgtat agccatgctc ttttgtatgt
      661 atatcaagca tgaaattaat tagtgctctt tccagtcttg ctccaagacc tttataaaga
      721 gaaaatcttg cgccagtgat ctttgctgcg gtttccagat cgaggattcc gagattcttt
      781 cctatctcca catggtcttt aatctcataa tcaaattttg ttggctcacc ccatgttttt
      841 acaaccgggt tatctgattc atctttacca actggtacgg cttcattcgt aatgtttggt
      901 atttgcagga gtatatttct cagatctgtt tcaataattg ttcttttatc atttaactct
      961 tttacagtat ctgacagctc ttttaaatca gcttgcagct gttttgcttc atcctttttt
     1021 ccttctctca tcaaactgcc aacttcttta gaccccttgt ttcgtttatg ttcaacctgt
     1081 tctgcctctt taattgcagt ttttctctta tctatcagtt cttttagagt atcaaagtct
     1141 acttttgtat ttctttgctc tgttttagtt attacaagat caaaattatc ttcaatgaat
     1201 tttatatcca gcatcttatt atccctttat aacacctatt ggtcttagtc tggctacttt
     1261 ttttgcaatc cctgcattat gaacaacgtc gcatacatct tcaacttttt tataagcatc
     1321 gggcatttct tcaacaattg ttcttttttt tctggctatg acttcaattc cttttttact
     1381 gagctctgca agcagcttat tcggatcgcc ctgcttaagc gctcttcttc tcgacagaag
     1441 ccttcctgct ccatggcagc aggttccaaa tgtttgctca agtgcgtttt cagttcctac
     1501 taaaacatat gatgctctac ccatatcacc cggtatcatg acaggctgtc caacgtcctt
     1561 gtaaatatca gggataagcg gatgattttt tggaaacgac cttgtagccc cctttctgtg
     1621 tacacatact tttttcattt caccattaat tttatgttct tctatttttg ctatgttgtg
     1681 gcttacgtca tagactagtg cagcacccag atctgcagag cttagattaa gcacattcat
     1741 aaaagcctct cttgtaaggt gcataattat ctgcctgttt atccatgcat aattcactgc
     1801 ggctgcaaag gcagagagat actcctttcc ttctttggaa ttaatgtgtg cgcaggcaag
     1861 ctgtttgtcg ggaagtttta tttgctctct ttccatatat ctcagcattt tattaatata
     1921 atcggagcat atctgatgcc caaaccccct tgagccgcag tgtatcagca cgcagacctg
     1981 cccgatggac aggccaaaag catcagcaac ctttaaattg tctatcttgt ccactacatt
     2041 aatctccaaa aagtggtttc ctgaaccaag cgagcctaac tgattccttc ctctttcaag
     2101 tgctttttgg cttatatttt ctaggtttga tcccgaaaaa tagcccctgt cttcaatcat
     2161 ctccaggtct tgttctattc cccatcctct ttctatcgcc catttcactc ctttatccgt
     2221 aaaatcgttg taatcactat cggataattt tattttaccc ccaatacccg ggccgcaggg
     2281 tacggttttg aatagttgct ctacaagaag tctaaggctt ggcctcacgt ctttctctgt
     2341 aagattggtt ctcattaaac gcacgccgca gttgatgtca tatcctaccc ctccgggtga
     2401 gatcactcca gtttcaatat ctgttgcggc aactcctcct attggaaatc cgtaacccag
     2461 atgaatgtcg ggcatggcta tggaatattt ttgtataccg gggaggtggg caacatttac
     2521 gacctgctcg attgatctgt cttcaataat agacttaagt attttctcgt ttacataaag
     2581 ccttccgggg acaagcatcc tgcctgtttt tggaatttca aaaagataat catttatctt
     2641 tattgttttt ataggtcgtg aatgttccat aaatttatat gtcaaatgtg atcgttgccg
     2701 ataataatcc gttattatgc tctacattta ggccactata tgttacggct tttatatcgt
     2761 ttaatagatc atgcctcgaa aaatcgatct cttcaccttt gcagataccc tcaacctctt
     2821 tattttcatt tactttaatt tcaaaatcag agaacaacag tcttttaact tcatgcatgt
     2881 atagcagttc tgaaagccag tttacaagga gttgttcttc tgagtttcca tctgatttaa
     2941 ccgcatattt attttttgct tcaatagtat tcaaatcgca aattatatcc atcattgcta
     3001 aagaactatt aattaataat tcgttaaagt ccctaccgga tactctaacg gatatatcgg
     3061 ctgtgtggtc aattatttca tagcttttat tcataacttt ataagaaaaa aataaattaa
     3121 ttattctgtc ttgcaaagtt ttatcatatt tgttaatttt acgaaatttt attctggagg
     3181 aaagaaatgg caaatatcgg taaaggcttt gagattacat attacggaca ttcaacattc
     3241 aggatcaaat cacccaaagg aaaaattatt ttcattgatc cctggataac agaaaacccc
     3301 tcatgccctg aaaaccttaa agatgttgat ggagtagata ttattgcagt tacccacggc
     3361 cattttgatc atataagtga tgttagacca cttgcagaga aatataaacc tactgttgtt
     3421 tcaaactggg agatttgtga ctggcttgga agtaagaaag ttgaaaactg tgcgccaatg
     3481 aataaagggg gagggcaggt agtagacgga ataaagtttt tcatgatcca tgctcagcat
     3541 tcaagcggca ttaaagacga tgacggttca actatatatg gtggagagcc tgctggcttt
     3601 gttattcaac ttgaaaatgg cttcaggctt tatcatgctg gagacactaa tatatttagt
     3661 gatatgaaat taatttccga gatatataaa cctgagcttg ttatgcttcc tattggagac
     3721 ttgtttacca tgtcaccgct tgaggcttct tatgcatgca ggtttttaaa tccaaaatat
     3781 gtgataccga tgcattatgg tacattcccg ccgcttatcg gcacacctga tcagctaaag
     3841 gctcttacaa gagaccttac agagatggag ataattgcac ttaaaccagg tgaggttttg
     3901 agctaggaat taaattcgga tttgaatttt tgaggcactc tgctgatttc tcttttattg
     3961 tagtcataaa atacaatgcc tgtctttact ctggctactt cctttagggt ctcagagtcg
     4021 gagagtttgt aaacaaaatc acacccgtat ttgctgaact cattaactgc aatctcaatc
     4081 tttaaggact gtccgtaaaa agcctctgat ttatatacaa gcgctgcgtc tgaaagaatg
     4141 atcccgggcc cgtcaatatt ggcttcggaa taacccaggc tttgcaagaa ccttacccgt
     4201 gcctcatgtg tcagcgaaag aattgagtca tgcgccaggt ggcctccata gtttatgtcg
     4261 cttattctaa gctttatttc agtgcagtat ataaagtcat tcggtagttc tatatttatt
     4321 cttgccataa tattcagtgt ttaggtttgt tgtaataata cggtaaaata gtttattggg
     4381 atcaacttat tatatacttt tt
//
LOCUS       DBNGSV010000336         4444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1446672, whole genome shotgun sequence.
ACCESSION   DBNGSV010000336 DBNGSV010000000
VERSION     DBNGSV010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1446672"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="AC159U_11840"
     CDS             <1..256
                     /locus_tag="AC159U_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3337785.1"
                     /translation="LGFTLNEIKQLIDLCKSNNAIGGDILKIVLKKINEIHSSLGELN
                     DNLHVLSVLASDCPVDVPALECPIILYLFESKIQKFSGGA"
     gene            257..502
                     /locus_tag="AC159U_11845"
     CDS             257..502
                     /locus_tag="AC159U_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337786.1"
                     /translation="MIFLADIAFALELTALILGTALLIFVSKENCSCKQFGKIIAYFT
                     IVASILAMLCTSYYSIRYWEEGAFDSTRVMMHKQMGN"
     gene            562..810
                     /locus_tag="AC159U_11850"
     CDS             562..810
                     /locus_tag="AC159U_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HIR3337787.1"
                     /translation="MEHSHDHYYKPEQQVRWWKRPSGMAAIFFFGAAGYFLVTEHWAH
                     IVPFLPWLIIILCPLIHLFHHGAHGSHGGHSSRDEEEK"
     gene            813..1481
                     /locus_tag="AC159U_11855"
     CDS             813..1481
                     /locus_tag="AC159U_11855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3337788.1"
                     /translation="MEHGMNNAYGLWSLVILNSLIFIIFAFSFFKPQTKRDWRSFSAF
                     SAFVVALFVEMYGFPLTIYLFSGWLTSKYPSVDFLSHENGHLLHTIFGFSGNPHFDPL
                     HIASFIFIGLGFLLLSSAWNVLYKAQREGKLAVTGLYARVRHPQYIGFVSIMFGFLLQ
                     WPTIITVIMFPILVVMYLRLALNEEQDCRERFGKAWDDYAATVPAFIPRFSSSQVSHE
                     GAKS"
     gene            1666..2049
                     /locus_tag="AC159U_11860"
     CDS             1666..2049
                     /locus_tag="AC159U_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337789.1"
                     /translation="MKSAKSLLTVFVFTLFVFTLAQGVSVSAAEQEEFIDLIGTAETP
                     EDHLKIADFYDAKALKMEKAANRHKSMGESYEKRSKPMMGMSKTCAKLSEQYMDSANS
                     YKEMAKHHRAMAGGTQDHDSHMHEH"
     gene            2148..3323
                     /locus_tag="AC159U_11865"
     CDS             2148..3323
                     /locus_tag="AC159U_11865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3337790.1"
                     /translation="MNLHSEIKNIDEPIKIVGAGPASLTAAINLANAGVEVEVYEKQK
                     DVGVRFNGDFQGIENWSTKIDALEFFRSIGLSINFLCKPFNEYEFYGPSLKSCKIKTS
                     RPLFYLVERGENEVSLDNGLKNQAIEAGVKFIWNKKIDKISGKMIVGTGPAAADVVAK
                     GIVFQTSHPDTCLGFLDNRLAQKGYAYLLVNQGRATFATVMFEEFKHINIYFDRAVDT
                     LNQVIKIDIQEQKEFGGYGNFTLNHTTERKSKVLYVGEAAGFQDALWGFGLRYAALSG
                     YLAAQSILHSKSYEQMCSEKIIPMMQTSFANRWLFAHLSNFGFEKALSRVSKTKDIIE
                     KLYKQYNPSAFKSLVFLVARQWYRSRVIDKQCLHEDCDCIWCRHCKEDYQYKEVLSN"
     gene            3404..>4444
                     /locus_tag="AC159U_11870"
     CDS             3404..>4444
                     /locus_tag="AC159U_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR3337791.1"
                     /translation="MVADFRKRFWVSLIITIPILILSPMIQTFLGLRESMQFTGDLYV
                     LFLFSSVVFFYGGYPFLKGIYDELRAWKPGMMTLIAIAITVAYLYSSAVVFGLSGKIF
                     FWELATLIDIMLLGHWIEMKSVMGASKALEELARLMPSDAHKVMQDGSVKDVPLSELN
                     SGDRVLVKPGEKIPADGEVIEGETSVNESMLTGESVPVSKKLSSKVIGGSVNGEGSIT
                     VEVQKTGKDSYLSQVVDLVRQAQESKSKTQDLANRAAVWLTIVALAGGAITIFVWLAI
                     AHKDFAFALERTVTVMVITCPHALGLAVPLVVAVSTAISASNGLLIRDRAAFERARNI
                     QAIIFDKTGTLTE"
BASE COUNT         1364 a          775 c         1014 g         1291 t
ORIGIN      
        1 gttgggtttt actctcaatg aaataaaaca gctaattgat ttgtgtaaat ctaataatgc
       61 aatagggggt gatattttga aaattgtgtt aaaaaaaata aatgaaattc actctagctt
      121 gggagaatta aatgataact tgcacgtact gagtgtttta gccagcgatt gccctgtaga
      181 tgttcccgca cttgagtgtc caataatttt gtatttattc gagtcaaaaa ttcaaaaatt
      241 tagtgggggt gcctaaatga tttttttagc cgacatagct tttgctttag aactaacagc
      301 cttaatttta ggaacggctt tattgatatt tgtaagcaaa gaaaattgtt cttgcaagca
      361 attcggaaag atcattgctt attttactat agtagcgtca atactggcaa tgctttgcac
      421 atcatattat tcaatacgtt actgggaaga aggtgccttt gattctacaa gggtaatgat
      481 gcataaacaa atgggaaatt aataagccaa gtagggtgtg gactagatga atgagtaatt
      541 tttcgaagga ggccataagt tatggaacat agtcatgatc attattacaa gcctgaacag
      601 caggtgagat ggtggaagag accaagcggc atggcagcga tctttttctt tggtgctgcg
      661 gggtattttc ttgtgacgga gcattgggcg cacatagtgc cgtttttgcc ctggttgatt
      721 attattttgt gtccgttaat ccatttgttt catcacggtg cacatggaag tcatggtggt
      781 catagtagcc gtgacgagga ggagaaatag tcatggaaca tggaatgaac aatgcatacg
      841 gattatggtc gctcgtaatc ttgaactcgc tgattttcat catatttgcc ttcagttttt
      901 tcaagccaca aaccaaacgg gactggcgaa gttttagtgc gttttcagct tttgttgtag
      961 cattgtttgt ggaaatgtat ggatttccgt taaccatcta cctgttctca ggatggctta
     1021 caagcaaata cccaagtgtg gacttcttat cacacgaaaa tgggcacctg ctgcacacga
     1081 ttttcggatt tagcggcaat ccacatttcg acccgctaca catcgccagt tttattttta
     1141 taggtcttgg ttttttactg ttgtcatcag catggaatgt gctttataag gcgcagcgtg
     1201 aaggaaaact cgcagtcacg ggtctttatg cgagagtgcg gcacccgcaa tatattgggt
     1261 ttgtatcgat catgtttggg tttctgctgc aatggccgac tatcataaca gtcatcatgt
     1321 ttccgattct ggtagttatg tatttgcggc tggcattaaa tgaagagcag gattgtagag
     1381 agcgattcgg taaggcttgg gacgattatg cagccacggt tccggctttt attccccgtt
     1441 tttcctcgtc tcaagtcagt catgaaggag cgaagtcatg aaacatgaac acaagtatta
     1501 caagtatgaa acgggaggta agtgtatgaa tattatatct taaattcagg acaggttatt
     1561 gaattcaaag gatcgatgag aagaagatta tatggattaa gtagaagata gaaaaaatta
     1621 gatagttaca aaaaaggaaa aaaataatat aaggaggtca ctttaatgaa aagtgcaaaa
     1681 tcattattaa cagtatttgt ctttacccta tttgttttta ctttggcgca aggagtttcg
     1741 gttagtgcag ctgagcaaga ggagtttatc gatttaatag gaactgcaga aactcccgag
     1801 gatcacctaa agatagctga tttctatgat gccaaggcgc tgaaaatgga aaaagcagca
     1861 aatagacaca aatccatggg cgaatcttac gaaaagagaa gtaaacctat gatgggaatg
     1921 tcgaagactt gtgcaaaatt gtctgaacaa tatatggact cagcaaattc gtataaagaa
     1981 atggcgaaac accatagagc aatggctgga ggtactcaag atcatgattc acacatgcat
     2041 gagcattaaa tattgctctt gttctggatc ctgcaaaaaa atattcagaa aaaaaacata
     2101 tgtaacaaca taagaacaaa ttaaagtaat aaatcaaaga gggatatttg aacttacatt
     2161 ctgagataaa aaatattgat gagcctatta aaattgttgg tgcaggtcct gccagcctca
     2221 ccgcggctat aaatcttgcc aacgctggtg ttgaagtaga agtgtatgaa aaacagaaag
     2281 atgttggcgt acgatttaat ggtgattttc agggaattga gaactggtcc acaaaaattg
     2341 atgcccttga attttttaga tcaataggtt tgtcaataaa tttcttgtgc aagcctttca
     2401 atgaatatga attctatggc ccgagtttga aaagttgcaa aataaaaaca tccaggccac
     2461 ttttttactt ggtagaacgc ggcgaaaatg aggtttcttt ggacaatgga ttaaaaaatc
     2521 aggcaattga agcgggcgta aaatttatat ggaataaaaa gattgataag atctctggaa
     2581 aaatgattgt tggcacaggc cctgcagcag ctgatgtagt tgcaaaagga attgtgtttc
     2641 aaacctctca tcccgacaca tgcctggggt ttttagataa cagacttgct cagaaaggct
     2701 atgcatatct tttagtcaat caaggcaggg ctacgtttgc aacagttatg tttgaggaat
     2761 tcaaacatat aaatatctat ttcgatcgtg ctgttgatac cttaaatcag gttataaaaa
     2821 ttgatatcca ggaacaaaaa gagtttggcg gatatggaaa ctttactcta aaccacacta
     2881 cagagagaaa gagtaaagtc ctttatgttg gtgaagcggc tggtttccaa gacgcattat
     2941 ggggatttgg tttaaggtat gccgcacttt caggatatct ggcagcgcaa agtatattgc
     3001 atagtaagtc ttatgagcaa atgtgcagtg aaaaaataat accgatgatg caaacatctt
     3061 ttgctaaccg ttggctcttt gcacatctaa gcaactttgg ttttgaaaaa gctctttcaa
     3121 gagtatctaa gactaaagat attatcgaaa aattatataa gcagtataac ccatcagcgt
     3181 tcaaaagctt ggtcttttta gttgcaagac aatggtaccg ctcaagagta attgataagc
     3241 aatgtctgca cgaagattgt gactgtatat ggtgcagaca ttgtaaggaa gattatcaat
     3301 ataaggaggt gttatcaaat tgagtcatca cgatcattct aatcatgaac attcgtcaca
     3361 tagacatcac gcaaaacatg accaccatga ccaccatgct catatggtag cagacttcag
     3421 gaaacggttc tgggtatctc tgatcattac aatacctata cttattctct ctccaatgat
     3481 tcaaacattt ttaggtttga gagaatccat gcagttcact ggagacctgt acgttctatt
     3541 cctgttttct tcagttgtgt tcttctatgg tgggtatcca tttcttaagg gcatatacga
     3601 tgaactgaga gcgtggaagc ccggtatgat gaccctgatc gccatcgcaa taaccgttgc
     3661 ctacctttac agcagcgctg ttgtttttgg attgtccggc aagatatttt tctgggaact
     3721 cgcaacactg atcgatatta tgctcctcgg gcattggatc gagatgaaat cggtaatggg
     3781 tgcctctaaa gcgctcgaag aattagcacg actaatgccg tctgatgcgc ataaagtcat
     3841 gcaggacgga agtgtcaagg atgtgccgtt gagtgaatta aattcagggg acagggttct
     3901 cgtcaaaccc ggcgagaaga ttcccgctga tggagaagta atagaaggtg agacgtccgt
     3961 caacgagtcc atgcttaccg gcgaatcagt tcctgtatca aagaaactga gtagtaaggt
     4021 tattggcgga tctgtgaatg gcgaaggctc tattacagtg gaagtccaaa aaaccgggaa
     4081 agactcttat ctatcacaag tggtagatct tgtcaggcag gcacaagaaa gcaaatcaaa
     4141 aacacaagat ttggccaacc gcgcagcagt ttggcttact atcgtcgcac tggcgggtgg
     4201 tgcaataacg atatttgtat ggcttgccat agcccataag gattttgcct ttgctcttga
     4261 gaggacagtg acagtgatgg tcattacctg ccctcatgca ctcggtttag ccgtgccgct
     4321 agtcgtggct gtatctactg ctatttctgc aagtaatggc ctgctgataa gagaccgggc
     4381 ggcgtttgag agagcaagga acatccaggc gattatcttt gataaaaccg ggacattaac
     4441 cgag
//
LOCUS       DBNGSV010000337         4870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_477539, whole genome shotgun sequence.
ACCESSION   DBNGSV010000337 DBNGSV010000000
VERSION     DBNGSV010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_477539"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC159U_11875"
     CDS             complement(<1..571)
                     /locus_tag="AC159U_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR3337792.1"
                     /translation="MKSISFVTLGCKVNQYDTAVLKNKLPKSKYTTVPFPQKADVYVI
                     DTCTVTHKADAEARHYINRAKRINPSGVVVVTGCYAQVSADEISQLKGVDYVVGNSDK
                     FSSLMKIVREGSVQDTPKVFISDIFKEKKRKFETPDIDFFPDRTRAFLKVQDGCNYAC
                     TFCIIPYARGRSRSLDIDDVLGRIRLLRES"
     gene            complement(571..1638)
                     /gene="mnmA"
                     /locus_tag="AC159U_11880"
     CDS             complement(571..1638)
                     /gene="mnmA"
                     /locus_tag="AC159U_11880"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3337793.1"
                     /translation="MTQRIMVAMSGGVDSATTAALLKSEGHEVIGVTMQLWDYGDAEG
                     GCCSVDEVRDARRVADQVGIPHYVVNYMDKFKEFILSDFINKYMDGKTPSPCVLCNEY
                     MKFDFLLKRSLELDADYLATGHYARIEKDDLGNYRLQKAVDSNKDQSYFLFTLKQKEL
                     SRVLFPLGDKTKEEVRSIAEGFGLKVAKKPESQNLCFITGSSYKDFLKDHTEASNTEG
                     DMVDTSGNIIGKHEGIFSYTIGQRRGLGYANGKPQYVVRIDTKNNKVVIGDEEDIYSK
                     TLIVNGLSWVCQVSDSEFEVNAKIRYRHKENIANLVINNKDEAIVNFHEPQRAITPGQ
                     AIVFYNDDKVIGGGWINEVLH"
     gene            complement(1721..2692)
                     /locus_tag="AC159U_11885"
     CDS             complement(1721..2692)
                     /locus_tag="AC159U_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HIR3337794.1"
                     /translation="MTEKRIKPIVVLSNCLEYKACRYNGELIPNSFVKQLEAFVIYKP
                     VCPEVEIGMGVPRDPIKIVLKNDKESLMQPSTGKDFSKPMSNFAKNYNASIEEVDGFI
                     LKSRSPSCGIKDVKLSDSMEKGPKIGNTTGFFAREVLNRFDGLAIEDEGRLTNYRIRE
                     HFLTKLYMLAEFRKLKKKKTIQALINYHTINKYILMSYNQTKMRELGKVIGSYDKSNI
                     DQIYESYERTLKTALKNPTRKSSNINMFMHVFGYFSKNLNSKEKSFFLESLQKYRNDK
                     LPVSSIITLLRSWVIKYEQEYLMEQTFFSPYPEELMNITDSGKGREL"
     gene            2765..3973
                     /locus_tag="AC159U_11890"
     CDS             2765..3973
                     /locus_tag="AC159U_11890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HIR3337795.1"
                     /translation="MDTLNISFRNRNNFELSARMDMPVGKPKAYAIFAHCFSCSKNIK
                     AAYHISRSLAGKDIAVLRFDFTGLGESEGDFSDTNFSSNVEDIIDAANYLSENYESPS
                     ILVGHSLGGTAITKAAVSIESSKAVVTIASPFDPSHVEKTITGGASDNNDQNYQITLG
                     GKSFNIKKQFIDNLKHSSIEDDLKRLNKALLVMHSPFDNVVSIDNASKIFLAAKHPKS
                     FISLDDADHLLSDQNDSVYVGKLISEWAGKYINLSGNKNTQAIDNKVVVQTGSGNLKT
                     DIFVGRHSMVADEPKSVGGTDLGPNPYDFLLASLGSCTTMTIKMYANRKGWKLDSAKV
                     IMDHKKIYDEDCGECETKTGKIDYIDRQLYLYGDLDEDQRNRLLEIADRCPVHRTLHS
                     EIVVKTKLLD"
     gene            4004..>4870
                     /gene="glnS"
                     /locus_tag="AC159U_11895"
     CDS             4004..>4870
                     /gene="glnS"
                     /locus_tag="AC159U_11895"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR3337796.1"
                     /translation="MNSENINKDFIREIVEQDIKDNKHGGNIVTRFPPEPNGYLHIGH
                     AKSICLNFGIAKEYGGICSLRFDDTNPAKEETEFVESIKEDVKWLGYDWQDRLYFTSD
                     YFDKLYEFALMLINDGKAYVDSLSMDEIREYRGTLIEPGKDSPYRDRSIEENLKLFKE
                     MKDGKFEEGEHVLRAKIDMASGNINMRDPVIYRIIKTPHHRTGSKFNIYPTYDFAHGQ
                     SDSIEGVTHSLCTLEFEDHRPLYDWFIKELDIFKSRQIEFARLNLSYTILSKRKLSEL
                     VKNKYVSGWDDPR"
BASE COUNT         1543 a          941 c          821 g         1565 t
ORIGIN      
        1 ctgattccct taaaagccta atacgaccta gcacatcatc tatatcaagg cttctcgatc
       61 tgccccttgc gtaagggata atacagaatg tacaagcata gttgcaccca tcctgcactt
      121 ttaagaatgc tcttgtccgg tcggggaaaa aatcaatatc aggtgtttca aactttcgct
      181 ttttctcttt aaatatatcg cttatgaaaa cctttggtgt gtcctgaaca gatccttctc
      241 ttactatttt cataagagat gaaaatttat cggaatttcc aactacatag tcaacaccct
      301 ttaactgact tatttcatct gcagaaacct gtgcatagca acctgtaact accactaccc
      361 cacttggatt tattctttta gccctgttga tatagtgccg ggcctctgca tctgctttat
      421 gggtaaccgt acaggtatca ataacataaa cgtcggcttt ttgaggaaat ggtacagtag
      481 tatatttact cttgggaagc ttgtttttaa gaaccgcagt atcgtactgg tttaccttac
      541 agccaagtgt tacaaaagag atgctcttca tcaatgaagc acctcattta tccatcctcc
      601 gcctataacc ttatcatcgt tataaaacac aatagcctgc ccgggtgtaa tagctctctg
      661 tggctcatgg aaattgacta ttgcttcatc tttattgtta ataaccaaat tagcaatatt
      721 ttccttatgc ctgtatctta tcttggcatt aacctcaaac tctgaatccg atacttggca
      781 tacccagcta agtccgttta caattaatgt cttgctataa atatcctctt catcaccgat
      841 tactacttta ttattctttg tatcaatcct aactacatac tgaggcttgc cattagcata
      901 accaagtcct ctcctctgac cgatcgtata tgagaatatt ccctcgtgct taccgattat
      961 atttccacta gtatctacca tatctccttc agtgttagat gcttcggtgt gatccttcaa
     1021 aaaatcctta tagctgcttc ctgtgatgaa gcacaggttt tgactctctg gtttcttggc
     1081 aacttttagt ccaaacccct ctgctatact acgaacctct tcttttgttt tatcaccaag
     1141 tgggaaaagt actctcgata gctccttttg ctttagtgta aaaagaaaat atgactgatc
     1201 cttattagaa tctactgcct tctgaagtct gtagtttcca agatcatcct tttctattct
     1261 tgcataatgt cctgtagcaa gatagtcggc atcaagctct aaagaacgct ttaagagaaa
     1321 atcaaatttc atgtattcat tacaaagtac gcagggtgac ggggttttgc cgtccatata
     1381 cttatttata aaatcagaaa ggataaattc cttaaattta tccatatagt ttacaacata
     1441 gtgaggaata cctacctgat ctgctactct tcttgcatca cgaacctcat ccactgagca
     1501 gcaccctccc tctgcatctc cataatccca gagctgcatt gtgactccta tgacctcatg
     1561 cccctcgctc tttaaaaggg ctgccgttgt ggcactatct actccaccac tcatagcaac
     1621 cattattctc tgtgtcataa ccggaggaaa tgttaccaga gattgtatta taaacaataa
     1681 ttactaacaa tattggtaag ttataaacca gaatttatta ttaaagttct ctgcccttcc
     1741 ctgaatccgt gatgttcatt agctcttcag gataaggact gaagaatgtt tgctccataa
     1801 gatattcctg ttcatatttt atgacccatg atctaagaag agtgattata ctgcttacag
     1861 gcaatttatc atttctatac ttttgcaaac tttccaaaaa gaaggatttc tcttttgaat
     1921 tcaggttttt tgaaaaataa ccgaatacgt gcataaacat gttaatattt gagctttttc
     1981 ttgtgggatt ttttagtgcc gtctttaatg tcctctcata actttcatat atttgatcta
     2041 tattgctctt atcataactt ccaataacct taccaagctc tctcattttg gtctggttgt
     2101 aagacataag aatatacttg tttatggtat gataatttat aagcgcctga atagtttttt
     2161 tcttctttag ttttctaaat tcagcaagca tatacagttt agtcagaaaa tgttccctta
     2221 tcctgtaatt cgtaagcctt ccttcatctt caatggcaag accatcaaat ctgttcaata
     2281 cttcccttgc aaaaaaacct gttgtattcc ctatcttggg acctttttcc atactatcag
     2341 ataacttcac atcctttata ccacaagatg gagacctgct ttttaatata aatccatcaa
     2401 cttcctcaat agaagcatta tagttttttg caaaattact catgggtttg gaaaagtctt
     2461 tccctgtaga gggctgcatg agactctcct tgtcattttt taaaactatc ttaatcggat
     2521 cgcgcgggac acccatacca atctctactt cgggacacac tggcttatat ataacaaatg
     2581 cttcaagctg ctttacaaag ctattaggaa taagctctcc gttatatctg caggctttat
     2641 actcaagaca gttgcttaaa actacaattg gcttaattct tttttcagtc atatttataa
     2701 tgatttattt aatttaatta tagcccctac aataatgctg taaaaataat ataaggacaa
     2761 ttttatggat actcttaata ttagttttag aaatagaaac aattttgaac tttcggcaag
     2821 aatggatatg ccggttggaa aaccaaaagc gtacgcaata tttgctcact gctttagttg
     2881 ttcaaaaaat attaaagccg cttatcatat aagccgctct ctggcaggaa aagatatcgc
     2941 agtattgaga tttgatttca caggacttgg tgagagtgag ggtgattttt ctgatacgaa
     3001 tttttcatct aatgtagaag acataataga tgctgctaat tatttatccg aaaactacga
     3061 gtcaccaagt attttagtag gtcactccct tgggggaaca gctatcacaa aggcagcagt
     3121 atctattgag agctcaaaag ccgtagtaac tatagcttcc cctttcgatc cttcacacgt
     3181 tgaaaaaact attacaggtg gtgcatctga taataacgat caaaattatc agataaccct
     3241 gggtggaaaa tcgtttaata tcaagaaaca atttattgat aatttaaaac actcaagcat
     3301 tgaagatgat ttaaaaagat taaataaagc acttcttgtt atgcattcac catttgataa
     3361 tgttgtgtcc attgataatg catcgaaaat atttcttgct gcaaaacacc caaaaagctt
     3421 tatatcactg gatgatgcag atcatttact atctgatcag aatgattccg tatatgttgg
     3481 aaaattaata tcagaatggg ccggtaaata tataaattta tcaggtaata aaaatactca
     3541 agcaatagat aataaagtag tcgttcaaac aggtagcggc aatctgaaaa ctgatatttt
     3601 cgtaggcaga cactctatgg tagcagatga accaaaatca gtaggaggaa ctgatctggg
     3661 tcctaaccct tatgactttc ttctggcttc tcttggttca tgtacaacaa tgacaattaa
     3721 gatgtatgcc aatagaaaag gctggaaact ggattctgca aaagtaatta tggatcacaa
     3781 gaaaatatat gacgaggact gtggagaatg tgaaaccaaa acagggaaaa tagactatat
     3841 cgacagacag ctctatctat atggtgatct tgatgaggat cagagaaaca gactcttgga
     3901 gatagctgac agatgtcctg tgcatagaac cttacattcc gaaatagttg ttaaaaccaa
     3961 attattagac taaaatccag ttcctcattt tatggagcaa ttgatgaatt ctgaaaatat
     4021 caataaagat tttataagag aaatagtaga gcaggatata aaagataata agcatggcgg
     4081 gaatattgtt acaagatttc cccctgaacc aaatggttat ctgcatatag gacatgccaa
     4141 atctatatgt ctcaactttg gtatcgctaa agaatacgga ggcatttgca gtctgagatt
     4201 cgacgataca aatcctgcaa aagaagagac tgagtttgta gaatctataa aagaagatgt
     4261 gaagtggctt ggttatgatt ggcaagatcg tctttatttt acatccgact attttgataa
     4321 gttatacgaa tttgcattaa tgctaattaa tgacggtaag gcttatgtgg acagtctttc
     4381 tatggatgaa ataagagaat acagaggaac tctcatagaa ccaggaaaag atagtcccta
     4441 tagagataga tctatagaag aaaatttgaa gctttttaaa gaaatgaaag atggtaagtt
     4501 cgaagaaggt gagcatgtat taagagccaa gattgatatg gcctctggca atataaatat
     4561 gcgtgacccc gtaatttaca ggataataaa gactcctcat cacagaacag gatcaaagtt
     4621 taatatttac ccaacatatg attttgctca tggtcagtct gactctattg aaggtgttac
     4681 ccattcacta tgcactctgg aattcgaaga ccacagacct ctttatgatt ggtttatcaa
     4741 agagcttgat attttcaaat ccaggcaaat agaatttgca agactaaatc ttagttacac
     4801 aatactaagt aaaagaaaat taagtgaact ggtaaaaaat aaatatgtat ctggctggga
     4861 cgatccccgg
//
LOCUS       DBNGSV010000338        10284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_824093, whole genome shotgun sequence.
ACCESSION   DBNGSV010000338 DBNGSV010000000
VERSION     DBNGSV010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_824093"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      33..124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            229..4977
                     /locus_tag="AC159U_11900"
     CDS             229..4977
                     /locus_tag="AC159U_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HIR3337797.1"
                     /translation="MCRLGGSLVLLKISLSVFLLISIFGDITSYSSEIIQNDIEDVDI
                     LSSTAVSGKANVLTLLDLSASMQTDFGGVGTGEWDGSDIIEACESFAGGTNTFDRRSF
                     ASHCAENVAGTSICGSQVCEIGVCDSQEEFDALVSCVIANQTDPSLLDSASIFTAVCQ
                     GPSASDCDRWWERVAAAASLEYGAISPNLTQCPSASSLTCQAAGDPDPGCDTTTDYAN
                     FKDCFDDLNSISPSQAQTCTGGILNCSGVAMSGSSRLDLAIDMFLEIFDADNSIESLS
                     CDDSTLNLFDGTSDTIDCMDYLETPFRDVSNIISGASNLPTSPAKNPIDELTSADSDF
                     INLRLRGMKFGGTDSSCTDSSAFEVETSGFSEGVDASGKQQRIRDLWTFYRDSQALGG
                     TPLALALGFDDMNDSTISASNLVVSDALNAFKNELETDSAVECRGQVAVVITDGEDSC
                     SGDCVSPNSIPSSCTGFTTTNANRRSAIQAVSNLRTYYARNPFTNNGQQFKKEIPVFV
                     VGLNIKNEKTQRMLHAMAMAGGTYAGDTLNGIDGGVIQHTGPDGNIIGDIDVASILPS
                     GSSYDVYKDIATAKNIDSNPSNAVLKQCTTPDNASNAKCQFAASTGDVNVFDDTFFTS
                     ATPFTPSDPLENFAYLANTPEELLAALKNIFQSAGSAFSGAVGSVPPVSTNAKSPDLR
                     GRIALTLNTPGSDHIWQGRLALYGLVDDPDNFGQQVIIRKPISTNSDLTDSDLVQSHK
                     IFTDAGVLKSAAKSFHWDVGKNLAELSPSSRNLKTVTSTDPTLITSSQNGILKYTSDL
                     ISFDKTIPHSAFGISDADVDTTTITSFCNTASTCSTDCSDQTSSACKSCVKDCLRDRV
                     VDFMRGHNEILPVNDPMGIKGFDIGSSCPDPSNPQPTDTYDTCSIKFSSYNSQPVFVG
                     SPPVLFSDIGFANFATSFRNRSAVMYVGSNAGFFEAIHAGNLVDFTDTTLTDSDKKNP
                     FTNIDETFDFYTAGQGREIFGFASPTFHTDSTAISSADTLLNNSSPDYRTGDFKSFVL
                     ENTEARSFYDSTPLIADIWIDGFKNGIKAKDAICDPDEAGADTDADLDGVIDPCGKEW
                     HTIAFIANKNGGGSVTALDITNPKCLNEACEHSLDGTVAITNLFADGTTYPSHLWTVF
                     DDDFGNTWSNPNVGKVRMKTENDDGNVTVDRWILFMGAGVDPADTDPTDGVSSGNAFH
                     AIDIASGKVVFKFHPTRDIPSELDMELENARMSNMTCDVAGIPGVFDINSDSYADLVY
                     VGDTCGRLWRFDVSMPLDHDSPSETVSDAGIKSDPFIEAADWTGDIVFCAAADESTCL
                     NTSMSTTQRQPIFFAPTLAFDSNGKKHLVFVTGNRRYPSVETQFGKLYNIIDEYIPAF
                     IAAGSPITTSIKTENDFDSEQIVDIVKQSGVSGQFVVNGDGISDPSNFGEFMIRFPDN
                     LDASDPASVIEDISGEKGIGTPLVINGLILFTTFAPGSSSGSTCGGELGKGRLFAVDF
                     QTGRSALAKVPGASDILKGTKIQKSAAAGVNTSEGIPSELRITRGSGGAPLVSISFTG
                     GPSTQGPKFLIWKLPQMPQETQMLYWEEVI"
     gene            4977..5804
                     /locus_tag="AC159U_11905"
     CDS             4977..5804
                     /locus_tag="AC159U_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337798.1"
                     /translation="MRILIKLSKYTIIYKFGALILLFNAYIFLVSQTAIPEDEQDNVV
                     LLVFDEDALDKGAALPDGSKPIEDLANNNPPNCGGGNVDVCINEDIKELNIRSFLFTR
                     GNDITDDSPAVQISLPTGQLGDEGIFKFFKDDDPQISDQNGAQFSKCEFITATGNASD
                     ENNLDKIVDVRPLTRQQIFDLINQQSYCTVALTSDASVTSPAENSGDLNGLPTTNLKG
                     ATRGITAFKVVNVVDHPDYIDDPTSSMLPIVTIELFPSNQIDKICERACVPRALSKH"
     gene            5820..6554
                     /locus_tag="AC159U_11910"
     CDS             5820..6554
                     /locus_tag="AC159U_11910"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3337799.1"
                     /translation="MKKKVHNKSGFTIIELLVAVLIISIGFLGLSQMGYLSLRQKHLA
                     ETGTDAANVIQFVTDRDLAEIKRLHLLNTKTYQDALTVKSLNLSYCDGDSEDPCAACP
                     CDPLNVLTTNTDPTPVGSADVITMCSVINIQDLDPDLIFNTDETTCRSNMNTQSADEN
                     EIMIMVREASTTVTNGTASTPSTVVIDMTYAVKSIQQFIETGLSTDLKDSLARDVFTV
                     TAHEENYSDDAVVLSGWTDVRIPHVP"
     gene            6544..7773
                     /locus_tag="AC159U_11915"
     CDS             6544..7773
                     /locus_tag="AC159U_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3337800.1"
                     /translation="MYHNNRYTSNTGGFTLLEILVSLAILSIIITGLFLLFMQTQRQF
                     NLTSQGIDVHQIGRSALDYIATEIRNAGARQGKTIAFRFFNGGGEDCNSNADSSKAGT
                     VNSPPDCLKIYSWDISRDQGSEGELNSIASSIETISFNPKLVLNAENWFQPKAAADLV
                     QSGDLVGIWARNARCDPTDNTGSSCSADPRGCTKCGAILKVDSVNTGLKQISFDDKNA
                     ILEQNFDVSGFTDLSVFVSDFYFKKIASVPSEMTLVKSREFTVNTTTNELLTKENDTG
                     PFVAIAGGPNQPGIIDLQFVFNLQDADGGRSRVGVKQDFDTREYFDFTIVDSANDVDL
                     RGREKDISTVEIYLLVRSRLKPQLLSGSGIPDKKIETIGDVAERDTSDPELGEGFIYK
                     IFSTTIYARNLIREEFG"
     gene            7846..8430
                     /locus_tag="AC159U_11920"
     CDS             7846..8430
                     /locus_tag="AC159U_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR3337801.1"
                     /translation="MNGQNTARINDAGQRGIAVFIALVMILVMSVMGVSLMFVSNTDF
                     QGMQALKRGQEAFLAAERCAEEGRFRVELNGFQNLLFQQQEGFKIVLGDGDSNKNINP
                     VCRTGNRRDDTAEEPYLAVTPDLKAKERPVENTSVPSGNVGGAVAIPLSFNVIGKYGT
                     DLDIVDSNPNINTGTELAIGVEVFMPAGVGSNIY"
     regulatory      8480..8578
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8610..>10284
                     /locus_tag="AC159U_11925"
     CDS             8610..>10284
                     /locus_tag="AC159U_11925"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR3337802.1"
                     /translation="MNTGNHLKAFCSNLYIYVSLFFLMSLFIFIQPSYSATITVNEMN
                     DAINGNGLCSLREAIINANNNNQSGSTACTAGQTGPGVVDVIAVPAGTITLSRFNASA
                     DDNANNGDLDITDEVRIIGQGTGPGATVIDGDCQLPYPWDTNNNTEPDCVNTEENLER
                     VFEVIENVGTSSDPVEFENLTIRGGFDDGNDDEAGGGGIYINHGTNVLIETAIIERNK
                     STARGGGIRNEGNTRINRTIIRENRSNNRGGGISNIGGPLDGQSTGEMLILFKCLISD
                     NYGVDNGGGISNEKDSNGSNNQCGTIEAYSSTIFWNVTDNQGAGIYQNGDCTDFEGHN
                     LTIVNNESEGIGGGISFADGNFVVSNTVIALNESTGSVQEDCSDAGTITATNSADSRS
                     GDGSNGSCFGSDAEDCTAAAGTGNKSNCDSTDLAFEGGANPTLADNGGPTPTLEILTG
                     SALIDMGDCTDTGIFTSPFDEDQTCCLRTNIGGSTCDIGAFEFDPTNTPIDDDSDRTQ
                     RETCAPTPTPTPTPPPPTPTPTPTPTPTPTPTPTATPTPTPTPTPPTPTA"
BASE COUNT         3220 a         1902 c         2306 g         2856 t
ORIGIN      
        1 tatacttgca ttgaattact tattaaatta atttctgata atggcacacc atttgaaaaa
       61 atgggccgca aaatcattgc ctaagggttt ggtcttttat tcctatggtt gaaagtttgc
      121 cggataatac tagggattga tagatattta ccaatcttaa atttgttgag attggaaatg
      181 tctataatta gtttaaggaa aaactctact gtaaaagagg ttagtattat gtgtcgactc
      241 ggaggcagct tagttctatt aaaaattagt ctttccgtat ttttattgat ctctatattt
      301 ggggacatca catcctactc ttctgaaata atacaaaatg atattgaaga tgttgacata
      361 ctttcttcta cagccgtaag cgggaaggca aatgttctca ccctactgga tttatccgct
      421 tccatgcaga ctgactttgg aggagtggga acaggagaat gggacggatc agatattatc
      481 gaagcatgcg agagctttgc gggcggtaca aatacatttg atagacgttc ttttgcatca
      541 cattgtgcag aaaatgtagc tgggacatcg atatgtggtt ctcaggtctg tgaaatcggt
      601 gtatgtgatt cacaggaaga gtttgatgcg ttagttagct gcgtaattgc gaatcagaca
      661 gatccttcat tgctagatag cgcttcgatt tttacagcgg tatgtcaggg accatcagcg
      721 tcagattgtg accgttggtg ggaaagggtt gcagctgctg catcacttga atatggtgca
      781 atatcaccaa atctgactca gtgcccttct gcttcaagcc tgacatgcca ggcagccggt
      841 gatcctgatc caggctgcga tacaactact gattatgcaa attttaagga ttgttttgac
      901 gatcttaact caataagtcc aagtcaggcc cagacctgta ccggaggtat acttaattgc
      961 agcggtgtcg caatgtctgg aagctctcgc ctcgatctgg caatcgatat gtttttggaa
     1021 atatttgatg ccgataactc tatagaatct ctgtcatgtg atgacagcac cttgaattta
     1081 ttcgacggta caagcgatac tatagactgt atggattatc tggaaactcc attcagagat
     1141 gttagcaata ttatttcagg tgcatcaaat ctgcccacat cccctgcaaa aaatcctata
     1201 gatgaactaa ccagcgcaga cagtgatttt atcaatctaa gacttagggg aatgaaattc
     1261 ggtggtacag atagcagttg taccgactcc tcggcatttg aagttgaaac aagcggtttt
     1321 tcggaaggag tagatgcttc tggcaaacag cagaggataa gagacctctg gactttttat
     1381 agagacagcc aggctcttgg tggaacccct cttgcccttg cactcggctt tgatgatatg
     1441 aatgactcca ctatttccgc aagcaacttg gtagtaagcg atgcgcttaa tgcatttaag
     1501 aatgaattag aaacagacag cgctgtggaa tgtaggggac aggtcgcagt tgtaataaca
     1561 gacggtgagg attcatgctc cggagattgc gtatcaccaa attctattcc atcatcctgc
     1621 acaggattta ctactaccaa tgctaacaga agaagtgcaa tacaggcggt aagtaatcta
     1681 agaacatact atgcaagaaa cccatttact aacaacggcc agcagtttaa aaaagaaatc
     1741 cctgtatttg tagtcggcct taatataaag aacgaaaaaa cacaaaggat gctccatgct
     1801 atggccatgg ctggaggtac ttatgcaggt gacacattaa atggaatcga tggtggtgta
     1861 attcagcata caggaccaga tggtaatata atcggtgata ttgatgttgc atcaatttta
     1921 ccatcaggta gttcgtacga tgtttacaaa gatattgcaa cagccaagaa tatagattct
     1981 aatccttcaa atgctgttct taaacagtgc acaacacctg acaatgcatc aaatgccaag
     2041 tgtcagtttg cagcgtccac cggcgatgta aatgtattcg atgatacctt ctttacaagt
     2101 gcaacaccat ttaccccctc agacccatta gagaattttg cttatctcgc aaacaccccc
     2161 gaagaacttc ttgccgccct aaaaaatata ttccagtctg caggaagtgc tttcagcggt
     2221 gcggtcggca gtgtccctcc cgtatctaca aatgcaaaat ctccagattt aagaggcagg
     2281 attgctctaa cactcaacac acccggatca gaccatatat ggcaggggag actagcgctc
     2341 tatggtttag ttgatgaccc tgataatttt ggtcagcagg taattataag aaaacctata
     2401 agcactaatt ccgatcttac tgattctgat ttagtccagt cgcataaaat ttttacagat
     2461 gcgggcgtgt tgaaatcggc tgctaagtcg tttcactggg atgtcggtaa aaaccttgca
     2521 gagcttagtc cgtcatcaag aaatctaaaa acagttacct ctactgatcc aacccttatt
     2581 acttcatcac aaaatggaat tttaaagtat acatctgatc ttataagttt tgataagaca
     2641 attccacatt cagcttttgg aataagtgat gctgatgtgg ataccactac aattacaagc
     2701 ttctgtaata ctgcaagtac ttgttcaact gattgctcag accagacatc ttcagcatgc
     2761 aaatcgtgtg ttaaggactg tttgagagat agggtagttg attttatgag aggccataat
     2821 gagatactac ccgtaaatga ccctatggga ataaaaggtt ttgatattgg aagcagctgt
     2881 ccggatcctt ctaacccaca gcccacagat acctatgata catgctcaat aaaatttagt
     2941 agttataact cgcagcctgt attcgtgggg tcgccgcctg tgttattttc cgatatagga
     3001 tttgcaaatt ttgctacaag tttcagaaat aggtcggctg taatgtacgt aggttcaaac
     3061 gcaggattct ttgaggctat acatgcgggc aaccttgttg attttacaga tactactttg
     3121 actgatagcg ataagaaaaa tccctttacc aatattgatg agacttttga tttctatact
     3181 gcaggacagg gaagagagat atttggtttt gcctcaccaa cattccatac agattcgact
     3241 gcgatatcat cagcggatac tttattgaat aattcttccc ctgattacag gacaggcgat
     3301 tttaagtcat tcgtattgga aaatactgag gcaagatctt tttacgattc tactccgcta
     3361 atagcggata tatggattga cggatttaaa aatggaataa aagccaagga cgcaatatgt
     3421 gatcctgatg aagcaggggc cgatacggat gctgatctgg atggagttat agacccatgc
     3481 gggaaagagt ggcacacgat tgcattcatt gctaacaaaa acggtggagg ttctgtaaca
     3541 gcacttgata tcacaaaccc aaaatgcctt aatgaagctt gtgaacattc actggatgga
     3601 accgttgcaa ttactaacct atttgcagat gggacaacat atccatcgca tctatggaca
     3661 gtatttgatg atgattttgg aaacacatgg tccaatccta atgtaggcaa ggtgaggatg
     3721 aagactgaaa atgatgatgg gaacgttacc gtagacaggt ggattctttt tatgggtgct
     3781 ggtgttgatc ctgctgatac tgatcctaca gatggagtga gttcaggaaa tgcttttcat
     3841 gcaattgata tagcaagcgg aaaagttgtg ttcaagttcc accctacgcg agatatacca
     3901 tccgaactag atatggaact ggaaaatgct agaatgagta atatgacctg tgatgttgca
     3961 ggcatacctg gtgtatttga tattaactcc gattcatatg cagatcttgt ttatgtgggt
     4021 gatacatgtg gaaggctctg gagatttgat gtgtccatgc ctcttgatca tgattcgcca
     4081 tcagaaacag tatctgatgc aggaattaag tctgatccgt ttattgaggc tgctgactgg
     4141 accggtgata ttgtgttttg tgcagctgcg gacgaatcaa cctgtttaaa cacttcaatg
     4201 tcaacaactc aaaggcaacc catattcttt gcacctacgc ttgcattcga ttcaaatggg
     4261 aaaaaacatc tggtgtttgt aactggcaac agaaggtatc caagcgtaga aacgcagttt
     4321 ggaaaacttt ataatatcat tgatgagtac ataccggcat ttattgctgc cggaagccct
     4381 ataacaacaa gtattaaaac agaaaatgac tttgatagtg agcagatagt tgatatagtg
     4441 aagcaaagcg gagtaagcgg gcagtttgtt gtaaatgggg acggtataag cgatccttca
     4501 aattttggag agtttatgat tagattcccg gacaaccttg atgcttctga tccggcgtca
     4561 gtaattgagg acatttctgg tgaaaagggt attggaactc cgcttgtcat taacggattg
     4621 atacttttta caacattcgc gccaggcagt tcttcaggca gtacatgtgg aggggagctt
     4681 ggaaagggca gattatttgc tgtagatttt cagaccggca ggtcagcctt agcaaaagtt
     4741 ccaggagcgt cagatatcct gaagggaact aaaatacaaa aatctgccgc agcaggtgta
     4801 aatacatcag aaggtattcc atccgagctt agaattacaa gaggctcagg cggcgcgcca
     4861 cttgtatcta tctcctttac cggtggacca agtactcagg ggccaaaatt tttaatctgg
     4921 aaactgccac aaatgcctca ggaaacacag atgctatatt gggaggaagt tatatagtga
     4981 gaatacttat aaaactaagt aaatatacta taatatataa atttggggca ctaattctat
     5041 tatttaacgc ttatatattt ttagtaagtc agacggccat ccctgaggac gaacaggata
     5101 atgtagtgct cctagtgttt gatgaggatg ctttggataa gggtgcagcg ctcccagacg
     5161 gctccaaacc tattgaagat cttgcaaata ataatccacc aaactgtggt ggtggcaatg
     5221 tagatgtgtg tatcaatgaa gacataaaag aattaaatat aagaagtttt ctatttacca
     5281 ggggcaacga cattactgat gacagccctg ctgttcaaat atctcttcct acaggtcaac
     5341 taggagatga agggatattt aaattcttca aagatgatga tccacaaata agcgatcaaa
     5401 acggagcgca gttttccaag tgtgagttta taaccgctac aggaaatgct tcagatgaga
     5461 acaatctgga taaaattgta gatgtaagac cacttacacg gcagcagatc tttgatctta
     5521 taaaccagca atcctattgt acagtagctc taacaagtga tgcgagtgtg acgagcccag
     5581 cagagaactc gggagattta aacggccttc caacaacaaa tttaaaaggt gctacaagag
     5641 gaatcacagc ttttaaagta gtgaatgttg ttgatcaccc tgattatatt gatgatccca
     5701 caagctcgat gcttccgatt gttacaatcg agctttttcc ttctaatcaa atagacaaaa
     5761 tttgcgaaag ggcttgtgtg cctagagcgc taagcaagca ctaacctttt atttaaatca
     5821 tgaaaaaaaa agtccataat aagtctggtt ttacaattat agaactgctg gtagcagttt
     5881 taattattag tattggtttt ttgggactat ctcaaatggg ctatttatcc cttagacaaa
     5941 aacatctcgc tgagacagga acggatgctg ccaatgtaat acagtttgtg actgatagag
     6001 atcttgctga gataaaacga cttcatcttc tcaacacaaa aacatatcag gatgcactta
     6061 ctgtaaaatc tcttaatctt tcatactgtg atggagattc ggaggatcct tgtgctgcat
     6121 gcccctgtga tcccctaaat gttctcacaa ctaataccga ccccacgcca gtgggttctg
     6181 cagatgtaat cacaatgtgc agtgtgatca acatccagga tttagaccct gatctaatct
     6241 tcaacactga cgagacaaca tgtagaagta atatgaatac tcaatcagcg gatgagaatg
     6301 aaattatgat tatggtgaga gaagcgagca ctacagtaac gaatggtacg gcaagcacgc
     6361 catccacagt cgttatagat atgacatatg cagtaaagag tattcaacag ttcattgaaa
     6421 ccggactatc gactgactta aaagattcac tggccagaga tgtttttacg gttacagcgc
     6481 atgaagaaaa ttattctgat gatgcagtag ttctgtccgg atggacggat gtgaggatac
     6541 cacatgtacc ataataatcg ttatacatcc aataccggtg gtttcacact tttagagatt
     6601 ttagtaagtt tagccatatt atctataata ataaccggtc tatttctttt attcatgcag
     6661 acacagcgac agtttaacct tacatcacaa ggtatagacg tccatcagat cggcagatca
     6721 gcacttgatt atatagctac agaaattaga aatgcgggtg caagacaggg taaaaccatt
     6781 gcttttcgat tctttaatgg aggaggcgaa gactgtaaca gtaatgctga cagctcaaaa
     6841 gcaggcactg ttaattcccc tccagattgt ctgaaaatat actcatggga tatatcaagg
     6901 gatcagggaa gcgaagggga actaaattcc atagcttcta gtatagagac aatctcattt
     6961 aatccaaagc tggtattaaa tgcagaaaac tggtttcagc caaaagccgc tgcagacttg
     7021 gtacagagtg gagatttggt tggtatttgg gctcgcaatg caaggtgtga tcctactgac
     7081 aatacaggat caagctgtag tgcagatccc aggggatgta ccaaatgcgg tgcaatactt
     7141 aaagtggata gtgtaaatac tggtttgaaa caaataagct ttgacgacaa aaacgcaatt
     7201 ctggagcaga attttgatgt aagtggtttt accgatttat ctgtatttgt aagtgatttt
     7261 tactttaaaa agatagcatc tgttccttct gagatgactt tggtaaaatc cagggaattt
     7321 acagttaata caactacaaa tgaactgtta acaaaagaaa atgatacggg gccttttgta
     7381 gctattgcag gcgggcccaa ccagccggga ataattgacc tgcagttcgt attcaatctt
     7441 caggatgcag acggcgggag gagcagagtt ggtgttaaac aggatttcga tacgagggag
     7501 tattttgatt ttacaattgt tgacagtgca aatgacgttg atttgagagg aagagaaaag
     7561 gatatatcaa ctgtggaaat ataccttttg gtacgctcca ggttaaagcc tcagctcctt
     7621 agtggttctg gcatccctga caaaaaaatt gaaactattg gagatgtagc agagagagat
     7681 accagcgacc cagaacttgg agaaggtttt atatataaaa tattcagtac aacaatatat
     7741 gcaagaaatc ttattagaga ggagtttggt tagcagaatc atgagataaa aattgcttag
     7801 taaaagaaca gccttaaatt atcttaattt gaaggagatt ttgagatgaa tggacaaaat
     7861 acagcaagaa taaatgatgc gggacaaaga ggcattgcgg tttttatagc tcttgtgatg
     7921 atattagtga tgtctgttat gggagtttca ctgatgtttg tttctaacac agatttccag
     7981 ggtatgcagg cacttaaaag aggacaagaa gcatttttag ccgctgaaag atgcgctgaa
     8041 gaggggaggt ttagggttga attaaatggt tttcaaaatc tgctgtttca gcaacaagaa
     8101 ggttttaaaa tagtgttagg agacggggat tcgaataaaa atattaatcc agtgtgtaga
     8161 acgggtaatc gaagagacga tacagcggaa gaaccatacc ttgctgtcac cccggatttg
     8221 aaggcaaagg agagacctgt ggaaaatact agtgtgcctt cagggaatgt gggtggcgcc
     8281 gtagctattc cgcttagttt taatgttata ggaaaatacg ggaccgatct cgacatagtt
     8341 gacagtaatc caaatattaa tacaggaaca gaacttgcaa ttggcgttga agtttttatg
     8401 cctgctggag taggaagcaa tatttactaa gaaagattga aaaaacaata taaagttagt
     8461 attattaacg tgaagtagtt acaagaaagg gcacactgtg tgaaagcaca ggccgcaaag
     8521 ccttattacc taagggcaaa attcaggttg tttagcctaa ggtcgtatgg ttaccgtgtt
     8581 tagattaaat ctaacttgga gtatggaaaa tgaatacagg caatcatctt aaagcatttt
     8641 gttctaatct ttacatatat gtttctctct tttttttgat gtcacttttc atttttattc
     8701 aaccatccta ttcagccaca atcacagtaa acgaaatgaa cgatgccata aatggtaatg
     8761 gcttgtgctc tctaagagaa gcaattatta atgcaaataa caataatcaa tccggttcta
     8821 cagcatgtac cgccgggcag acaggtcctg gcgttgtaga tgtaatagcg gtgccagccg
     8881 gaacaattac attatcaaga tttaatgctt cagcagacga taatgcaaac aatggtgatc
     8941 tggatataac agatgaagtt cggattatag gccagggaac cgggccggga gctactgtta
     9001 tagatggaga ctgccagctg ccatatccgt gggacacaaa taataataca gaaccagatt
     9061 gtgttaatac tgaagaaaac ctggaaaggg ttttcgaggt catagagaat gtaggcacct
     9121 cttcagaccc cgttgagttc gaaaatttaa caattagagg tgggtttgat gatggaaatg
     9181 atgatgaggc cggtggtggt ggtatatata taaaccatgg cacaaatgtt ttgattgaaa
     9241 ctgcaataat agaacgcaat aagagtacag cacgcggagg cggtataagg aacgaaggaa
     9301 acacgagaat aaacaggact attataagag agaacagatc aaataacagg ggaggaggaa
     9361 taagtaatat tggcggacct cttgacggtc aatccacggg tgaaatgcta atactcttta
     9421 aatgccttat ctcagataac tatggagtcg acaacggcgg tggtatctct aatgaaaaag
     9481 acagcaatgg tagtaacaat cagtgtggaa ccatagaagc ttatagtagt acgatttttt
     9541 ggaacgttac tgacaatcag ggtgcgggta tttatcaaaa tggtgattgt acggactttg
     9601 aaggtcataa cttgactatt gtaaacaacg aatctgaggg tattgggggt ggaataagtt
     9661 ttgctgatgg aaactttgta gtaagcaata ctgttattgc actcaacgaa tctacaggat
     9721 ctgttcagga agactgctca gatgctggta ctataacagc tacaaacagt gcagactcaa
     9781 ggtctggaga tgggagcaac ggaagctgtt ttggtagtga tgcagaggac tgtactgccg
     9841 ctgctggtac gggtaataag agtaattgtg attctacaga tcttgcattt gagggtggag
     9901 ctaatccaac cctcgctgac aatggcggac caacgccaac acttgaaata ttaaccggta
     9961 gtgcattgat agatatgggt gattgtacag acactggcat tttcacatca ccttttgatg
    10021 aagatcaaac ctgttgttta agaactaata taggtggaag tacatgtgat atcggagctt
    10081 ttgagttcga tccaactaat acaccaatag atgatgacag cgataggact caaagggaaa
    10141 cctgtgcgcc gactccgact cctacaccga caccaccacc gcctacacca actccgaccc
    10201 ctacaccaac acccacgcct acgccaacgc cgactgctac gccgactccg actcctacgc
    10261 cgacaccgcc aacacctaca gcaa
//
LOCUS       DBNGSV010000339         5260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1239574, whole genome shotgun sequence.
ACCESSION   DBNGSV010000339 DBNGSV010000000
VERSION     DBNGSV010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1239574"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..641
                     /locus_tag="AC159U_11930"
     CDS             276..641
                     /locus_tag="AC159U_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3337803.1"
                     /translation="MPIYEYECKKCGDIIEAIQGFNDAPLRKHKECGGKLERMISLSS
                     FHLKGSGWYDTEYGKKKVKRGTQSAEKSDRTDGEGKSDSDSKDSSSSADTSTASNDSG
                     SSTADSIKKDTAKAKNASK"
     gene            645..1436
                     /locus_tag="AC159U_11935"
     CDS             645..1436
                     /locus_tag="AC159U_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3337804.1"
                     /translation="MELGLKDKVAIVPASSKGLGRAVAMGLAAEGAKLTICSRNEDDI
                     NKTAKEIIDTTGSQVLALNADVSDQKELEYIVNKTTDTFGTIHILINNAGGPPFGYFD
                     QFDMEQWQKAIELNLFSTIRLTQLVTPYMIDNLWGRIINITSIAVKQPLDGLILSNTS
                     RAGVTGFAKTISNELAKHNILVNNVCPGRIFTDRIKSLADSRAKQEGKSFEQIIEAMQ
                     SDIPVGKIGTPEEFANMVVFLASEKASYITGNTIQIDGGLLKGIF"
     gene            1450..1974
                     /locus_tag="AC159U_11940"
     CDS             1450..1974
                     /locus_tag="AC159U_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3337805.1"
                     /translation="MINVIKYFKNIDPVIILILFTILFYLLTRIEILPIYKTDTISTT
                     KDYTIQIQKDYEYPVVYQFNNNELPKEFKYASFKKKLLRDGNKYLLNDNNIIIETSEI
                     NGKHKIALGIPININTAKLEELTALPSIGQVTANNIIDYRIKVNKITDLEDLINVDGI
                     GRDKINKIKELIEF"
     gene            complement(1971..4577)
                     /locus_tag="AC159U_11945"
     CDS             complement(1971..4577)
                     /locus_tag="AC159U_11945"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3337806.1"
                     /translation="MFRKLFKYFFYLSVITVIIGSVAAYLGYRYYTRDLPDFRDNISS
                     YKPKLINQFYTSEGYLLAEFYSENRRLIPFTDIPQHVIDAFVAVEDRRFYEHEGLDYQ
                     GIFAALVENIREGSIKRGGSTITQQVTKNIVLSPERSFSRKIKEAILAYRIEKNLSKQ
                     EILYLYLNHIYLGDGAYGVEAASKNYFGVSVKEISIAQAAVLAGLPKRPERYNPRKNL
                     QRALERQRTVLRILQSTQHITDEQYEQAKNEEIVLSAKKNINNNISPYFVEYVRKYLE
                     YSYGTASYLEGGYRVFTSMDTDLSLKGQWAVRKGILDLEKRRGRKEIIRSINDNSIET
                     YINNQGETEIENNKIYEAVVVNVNKLDGSSFSIASINIANNKGIIKYVVPKQIDKKIK
                     ELPFEYSKKYSPISGYDDIDILDRSLKKGDIINVKVIGKDEDRFIAKLYHKPLTQAAL
                     YSMDTNGFIRAMIGGYDFTQNQFNRSTQALRQPGSAFKPILYASALDKGYTASTMVYD
                     IPVVMDEWSPENYDETYLGPIILKHALAKSRNLASIRLILNLTPAYVVDYAKYFHFKS
                     KLNPYPSLALGGSEATLFEMVNAYNVFATNGRYIEPKFVYRIYDRNGKIIEDNTEGVF
                     FSKEQAYRADRDIKREKIIKGIAKNLGRDSHINTSDSFLKENNLTSKFYKEEITSYNA
                     PLDFRDLLLNSQNLPFINSNLGSQVISQESAFIMTHMLEAVVKEGTAKRAKPLLDKAP
                     IAGKTGTTNDFTDAWFVGFSPKVVTGVWVGTDNYKPLGKKESGSKAALPIWIDYMDAA
                     LNKYPGGEFEQPEGVKFIATPYGNLPFNTSTCTNQRCEDYITQGVGYRDNSIEEQFKS
                     DTLNEADFLMRH"
     gene            4697..4903
                     /locus_tag="AC159U_11950"
     CDS             4697..4903
                     /locus_tag="AC159U_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337807.1"
                     /translation="MSTENEIITLDSFVRSQTNQELKGLLLKLKNELRKEDTLWEDIK
                     DILIKVNEFDNKLLAVIIPLIISE"
     gene            4936..>5260
                     /locus_tag="AC159U_11955"
     CDS             4936..>5260
                     /locus_tag="AC159U_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3337808.1"
                     /translation="MNFSKSYSNLISLIGNTKIIRLNRVIPSGYAAVWAKLEQFNPSG
                     SVKDRICIKIIEEAEKSGLIKPGIHTIVEATSGNTGIGLAMICKIKGYKLILTMPDDV
                     SYETIQ"
BASE COUNT         1776 a          955 c          860 g         1669 t
ORIGIN      
        1 ctccacgagc tgttatatca gataataaat ccaaaaacta tgagcaaaca tgtacttcaa
       61 ctcaatgaca aaatatcagc aaattctaac tctaaaggtg gacctgaatt ctggggcttg
      121 acaaaaagca cggtcaaaga aatctaaata gtacaactac cctacaagtt accaccctcc
      181 cccataactc attaataatc caacttagta ttgaaactat cagactagtt attatattat
      241 aatcctttta ttaagtaacc aggagtatta cggaaatgcc tatttatgaa tatgaatgta
      301 aaaagtgcgg agacataatt gaagccattc agggctttaa tgatgctcca cttagaaaac
      361 acaaagagtg tggcggtaag cttgaaagga tgatatcact ttcatctttt cacttaaaag
      421 gaagcggttg gtatgatacc gaatacggca agaaaaaagt taaacgtgga acccagagtg
      481 ctgaaaaatc cgacaggacc gatggtgagg gtaaatccga ttctgattca aaagatagtt
      541 cttcttccgc tgatactagt acagcctcta atgatagcgg ttcatcaact gcagattcta
      601 tcaagaaaga cacagctaag gcaaagaatg cctctaaata gattatggaa cttggcctaa
      661 aagacaaagt agcaatagta ccggcatcaa gtaaagggct tggcagagca gttgctatgg
      721 gccttgctgc agagggagca aagttaacaa tctgttcaag aaatgaagat gacataaata
      781 aaactgcaaa agagattatc gatacaaccg gaagccaagt acttgccctt aatgcagatg
      841 tatcagacca aaaagagtta gaatacatag ttaataaaac aacagataca tttggaacca
      901 ttcatatact cataaataat gcaggcggtc ctccttttgg atacttcgat cagttcgata
      961 tggagcagtg gcaaaaggca atcgaactaa acctctttag cactatcagg ctcactcagc
     1021 ttgtaacacc ttacatgata gataatctgt ggggtcgtat cataaatatc acttccatag
     1081 cagtaaagca gccgcttgac ggactcatac tttctaatac atctcgtgcc ggagtaactg
     1141 gttttgcaaa aacaatatcc aatgaattag ctaaacataa tattcttgta aacaacgtat
     1201 gcccgggaag aatctttact gaccgtataa aaagcctcgc tgactctaga gcaaaacaag
     1261 aaggtaaatc atttgaacaa ataattgaag ccatgcaatc agatattccc gttggcaaaa
     1321 tcggcacacc cgaagaattt gccaatatgg tggtatttct agcctcggaa aaagcaagct
     1381 atataacagg aaacactata cagatagatg gtggtttact aaaaggaata ttttaactat
     1441 aaaattacga tgataaacgt catcaaatat tttaagaata ttgaccctgt aattattctt
     1501 atcttattta ctattctttt ttatcttctt acacgcatag aaatattacc aatatataaa
     1561 acagatacca tcagcacgac taaagattat actatccaaa ttcaaaaaga ttatgaatac
     1621 cctgtagtat atcaatttaa taataatgaa ttacctaaag aatttaaata tgcttcattt
     1681 aagaaaaagt tattacgtga tggtaataaa tacttattaa acgataataa tataatcata
     1741 gaaacatctg agataaacgg gaaacataaa atagcgctag gaattccaat caacatcaat
     1801 acggctaaat tagaagagct aaccgcccta ccctccattg gacaagttac tgcgaataat
     1861 attatagatt acagaatcaa agttaataag attacagatt tagaagattt gattaatgtt
     1921 gatggaattg gcagagataa aatcaacaag attaaagaac ttatagagtt ttaatgtctc
     1981 attaaaaagt ctgcctcatt tagagtgtca gacttaaact gctcttcaat gctattgtct
     2041 ctataaccta caccttgagt aatgtagtct tcacatcgtt ggtttgtgca tgttgaagta
     2101 ttaaaaggca ggttaccata tggagtagca ataaacttta caccttcagg ctgctcaaac
     2161 tcaccacccg gatatttatt aagagcagca tccatataat ctatccatat tggaagtgca
     2221 gctttagaac ccgattcttt tttgccaagt ggtttatagt tatctgttcc tacccataca
     2281 cccgttacaa ccttagggct aaatcccaca aaccatgcat cagtaaagtc atttgtggta
     2341 ccagttttgc cagctatagg agctttatcc aaaagaggct ttgctctttt cgctgtacct
     2401 tctttcacta cagcttctaa catatgcgtc attataaagg ctgattcttg tgatataacc
     2461 tggctgccaa ggtttgaatt aataaatgga agattttgtg aatttaaaag aagatctcta
     2521 aaatcaagag gagcattgta gcttgtgatc tcctctttat aaaattttga agtcagattg
     2581 ttttctttta gaaagctatc agatgtattg atatgagaat ctcttccaag attctttgca
     2641 atacccttta ttattttctc tcttttaata tccctatctg ctctgtatgc ctgttctttt
     2701 gaaaagaata ctccttcggt attatcttcg ataattttac cattcctgtc gtatatcctg
     2761 taaacaaact tgggttcaat gtatcttccg ttagtagcaa acacattata agcatttacc
     2821 atttcaaaaa gcgtggcttc agaaccacca agagcaagag atgggtatgg attgagtttg
     2881 gatttaaaat gaaaatactt tgcataatca actacataag ccggagtaag attgagtatt
     2941 aatcttatag aagccaggtt tctggatttg gcaagagcat gttttaaaat aataggacca
     3001 agataagttt catcataatt ttccggtgac cattcgtcca ttacaaccgg aatatcatag
     3061 accatcgttg atgctgtata acctttatcc agagccgaag cgtaaaggat tggcttaaac
     3121 gcagaaccgg gttgtctaag agcctgagtt gatctgttaa actgattttg cgtaaaatca
     3181 taccctccaa tcatggccct tataaaccca tttgtatcca tagaataaag tgcagcctga
     3241 gttagaggtt tatgatataa cttagcaata aatctatctt catctttacc aattaccttt
     3301 acatttatta tatctccttt ctttaatgat ctgtctagaa tatctatatc atcatagccg
     3361 cttattggtg agtatttttt tgaatactca aatggcagct cttttatttt cttatcaatt
     3421 tgcttaggaa caacatattt gataattccc ttattatttg cgatattaat tgaggcaata
     3481 ctaaaactag aaccatccaa tttgtttaca tttactacta ctgcttcata tattttatta
     3541 ttttctatct cagtttcacc ctgattattt atatatgttt caatactatt gtcatttata
     3601 gatcttatta tctcttttct gcctcttctt ttttcaagat cgagtattcc ttttcttact
     3661 gcccactgtc cttttaaact aagatccgta tccatagatg taaataccct gtatccacct
     3721 tcaagatatg atgctgtacc atatgaatac tcgagatatt ttctcacata ttcaacaaaa
     3781 tatggagata tattattatt tatattcttc tttgcactaa gcactatttc ttcattcttt
     3841 gcctgctcat actgttcatc tgtaatatgc tgtgtgcttt gcaatattct aagcactgtc
     3901 ctttgtcttt ctaaggctct ctgtaaattt tttcgtggat tgtaacgctc tggtcttttt
     3961 ggaagtccgg caagtactgc agcctgtgct atgctaattt cttttacaga tacaccgaaa
     4021 tagtttttgc tcgcagcttc aactccataa gcgccatcac caaggtagat atggttaaga
     4081 taaagatata atatttcctg ttttgataaa ttcttctcta tcctgtaggc aagtatcgcc
     4141 tcttttattt ttcttgagaa gcttctttct ggagaaagca ctatgttctt tgtaacctgt
     4201 tgggttatag tgctgccgcc tctttttatt gagccttctc tgatattttc aacaagtgct
     4261 gcaaaaatac cctggtaatc caggccttca tgttcataaa atctcctatc ctcaacagca
     4321 acaaatgcgt caataacatg ctgcggaata tcggtaaaag ggataagcct tctgttttct
     4381 gaataaaact cggcaagcag ataaccttct gatgtataaa attgatttat cagttttggc
     4441 ttatatgaag aaatgttatc tctgaagtcc ggaaggtcac gggtatagta tctgtagcca
     4501 aggtatgcag caacagagcc tattattact gttattacgg atagataaaa gaaatactta
     4561 aataattttc taaacattgt gtattatata caattattac taattttgag tatgataaca
     4621 agaaaatgat taaaaataat aaaactacag tataatttat gtaattatta ttatattaga
     4681 tataatagaa cctcctatga gcactgaaaa cgaaattata acactagaca gttttgtaag
     4741 atcacaaact aatcaggaac ttaagggcct tctccttaaa cttaaaaatg aattaagaaa
     4801 agaagataca ttatgggaag atattaaaga tatactaatt aaagtaaatg agtttgataa
     4861 taaattgtta gccgtgatta ttcctctcat tatatcagag taatacctgc cgtctttaaa
     4921 ttacaataca tacacatgaa cttctcaaaa agctattcta atcttataag tcttattggc
     4981 aacacaaaga taataagact taaccgggtt ataccttcag gatatgcagc tgtttgggca
     5041 aaacttgaac aatttaatcc aagtggaagc gtcaaggaca gaatatgcat taaaattatt
     5101 gaagaagctg aaaaaagtgg cttaataaag cctggcattc atacaattgt agaagcaact
     5161 agcggcaata cgggaatcgg gcttgccatg atatgcaaga taaaaggcta caaacttatt
     5221 ttaaccatgc ctgatgatgt aagttatgaa acaatacagg
//
LOCUS       DBNGSV010000340         1386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1309371, whole genome shotgun sequence.
ACCESSION   DBNGSV010000340 DBNGSV010000000
VERSION     DBNGSV010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1309371"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="AC159U_11960"
     CDS             <1..1047
                     /locus_tag="AC159U_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337809.1"
                     /translation="IEHLEEKEQERIDMLEELRLEMSNVSANPEQFKKLDLEKYVSLW
                     TQKVKPSAPIPKVINFMDCKPVEVEIILRVGFIQIPKGITCCIHAPGGAGKSILMQRV
                     IMASGIRACYISGEDSPGLMANLLQPYKRFYSNYNADFVHFDNQDIGGVLEFIEYSDY
                     ELYVVDPLSSFCTGDKWGRVETENGVASELLRIMNGITAKTRKTIIYTHHEAKSTNDI
                     RTAARGSSALVDNARMGISINRLSERYRKPHSAKKTKYQEQEEHTSNVKCFQRALTRE
                     LSKQPQSVFKNQDMIGVVEMVCHKNNYGSVGDMAQFILTANIGRPGLNVVPAGGLQIE
                     LREAISDSFMIQEY"
     gene            1127..>1386
                     /locus_tag="AC159U_11965"
     CDS             1127..>1386
                     /locus_tag="AC159U_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337810.1"
                     /translation="MTNIEIEKKLLEVGGIVWEKNNMKRIYLNIDSILLLSEISHTED
                     QIKKAKKQKAYYNLIDGEFYAENGIVTRNVLRAAFDVSINKI"
BASE COUNT          544 a          178 c          283 g          381 t
ORIGIN      
        1 attgaacact tagaggaaaa agagcaagaa cgaatagaca tgctagaaga gttaaggctc
       61 gaaatgtcta atgtttctgc taatcctgag cagtttaaaa agttagattt agagaagtat
      121 gtatctttgt ggactcaaaa agtaaaacct tcagcaccga tcccgaaagt aataaacttt
      181 atggattgta aacctgttga agtagagata attttaaggg ttggatttat acagatccct
      241 aaaggaataa catgttgtat tcatgctcca ggaggtgcag ggaaaagcat tttaatgcaa
      301 agggtaatta tggcaagcgg aatcagagct tgttatatat ccggggaaga tagtccgggg
      361 ttaatggcta atttattgca accgtacaaa agattctatt caaattataa tgctgacttt
      421 gtacactttg ataatcagga tattggtgga gtgttggagt ttatagaata ttcagattat
      481 gagctttatg ttgtcgatcc attatcaagt ttctgtacag gtgataagtg gggaagggta
      541 gaaacagaaa acggtgttgc atcagaacta ttaagaatta tgaatggtat tactgctaag
      601 actcggaaaa ctataatcta cactcaccat gaggcaaaaa gcacaaatga tataagaaca
      661 gcagcaaggg gatcaagcgc attagttgat aatgcacgaa tgggaatatc tataaacaga
      721 ttatcagaga gatatagaaa acctcattca gcaaagaaaa ctaaatatca agagcaagag
      781 gaacatacat caaatgtcaa atgtttccaa agggcattga caagagaatt aagcaagcag
      841 ccacaaagcg tatttaaaaa ccaggatatg attggagtgg tggaaatggt atgtcataaa
      901 aacaactatg gaagtgttgg agatatggca caatttatat taactgctaa cattggaaga
      961 cctggattaa atgttgttcc tgccggtgga ttgcaaatag aattaagaga agctatttca
     1021 gattcattta tgatacagga atattaaatc tccttgtaat ctacacatta acttcatatg
     1081 aaccacaaac agaagatcta tatttagtac agataggaga gacaagatga caaatataga
     1141 aatagaaaaa aaattattag aagttggtgg tattgtttgg gaaaaaaata acatgaaaag
     1201 aatatatcta aatattgatt cgatattgtt attatcagaa atatcacata ctgaagatca
     1261 aattaaaaaa gccaagaaac aaaaagcata ttacaattta atagatggag agttttatgc
     1321 agaaaacgga atagttacaa gaaatgttct aagagctgca tttgatgttt caataaataa
     1381 aatata
//
LOCUS       DBNGSV010000341         6637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1170849, whole genome shotgun sequence.
ACCESSION   DBNGSV010000341 DBNGSV010000000
VERSION     DBNGSV010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1170849"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC159U_11970"
     CDS             <1..187
                     /locus_tag="AC159U_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337811.1"
                     /translation="NQKRPGRRDLHFLNRDGMVACNPRDHEAAHRAEVEGIATENKEA
                     LTCKKCLVEIRRELVTK"
     gene            302..1126
                     /locus_tag="AC159U_11975"
     CDS             302..1126
                     /locus_tag="AC159U_11975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3337812.1"
                     /translation="MSIFAIIFTALSFLFGLIFLIDCIRYRKQINEFVKYGICLGLIL
                     VSIDIAIYYLISNYLSDIHIVILVLSEFLSLVQITIFTCMGMYICKQLNFKDIPFVRS
                     FFEKSYNINRNYIFQKRFLVYVFSVIYLSVAYSIILFSLTSPEMSQTLKHMSESQLEE
                     LPNLNKPSFLAALVVLEFAFAEEIIFRLGIQNYIQKMLNLIDSQYWISISLTSLFWSF
                     AHANVLDPEWVKIVQIFPIGLGLGYLFNKFGTEACILAHGFFNIIMMFLTPYLFTI"
     gene            1395..1937
                     /locus_tag="AC159U_11980"
     CDS             1395..1937
                     /locus_tag="AC159U_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337813.1"
                     /translation="MIWKKFKDKHISYSSLISESKKYFSNNIGIGLAIGFTGFIVALF
                     IIVIVGVFSKDIDNRPDYTDFSIIVPLYFYLLLTIFCYAIPYLYVKEMNYKNAFFNSP
                     KILINHIKQSFPIILLLFINLVISFSTSYDQSYYFSDIIIEIIIYYISFNIFIITCQI
                     LDEIDISDFENGSTQNRKII"
     gene            1906..2574
                     /locus_tag="AC159U_11985"
     CDS             1906..2574
                     /locus_tag="AC159U_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337814.1"
                     /translation="MAQHKTGKSFNQVQGVRANRYSGSKLTNWMDFNPAGSSLYKIYS
                     DHYNEFINNKNKISYFKQKTHPDFIMPLKISGIKKYLQNVPEKYYKDIKAIFLLSGSN
                     KQEKVFFSKLFCYGTYMANCIFIHPYPKNNMNLIFKNPPKPNILNDYRRVGALIEKDD
                     TKGTILKWTKESLKDFYYRDVLMHEIGHHYDKYVWVKSNKKAEGFAEWFATEYGFKFK
                     HLKD"
     gene            2594..3340
                     /locus_tag="AC159U_11990"
     CDS             2594..3340
                     /locus_tag="AC159U_11990"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006307 - DNA alkylation repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3337815.1"
                     /translation="MYKSLITKLTNQINQHSNPAEKSWFENYIKNSAPFRGVKMPVVR
                     KELHKWHKDYIDGEMDLDQQLDLAFALIEEKYTEDKIAGTMFMQEILIPKGIMKPKRN
                     IKRLSELFSNGHIYDWNVCDWFCMKVLGELIVKDEKYWARPIHMWHSAENLWQARASL
                     VSFERVSDNQDYFSMIEKSCKKLIKREERFAKTAVGWLLREISKQDEDFVKQFIEHHI
                     KHFTAETIRNALKYSSKDEKKHYLTLLKSV"
     gene            complement(3355..3666)
                     /locus_tag="AC159U_11995"
     CDS             complement(3355..3666)
                     /locus_tag="AC159U_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3337816.1"
                     /translation="MFVTMNRIFVNEGYTDGFEQRFKNRAHEVDKMKGFVRNVVLKPK
                     GPEQPYVVMTFWESEDDFNNWVKSDAFIKGHAKSGTLPKEAFSTESHLETFDVFLDTE
                     A"
     gene            3980..4339
                     /locus_tag="AC159U_12000"
     CDS             3980..4339
                     /locus_tag="AC159U_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337817.1"
                     /translation="MKKWLVAPLIIFILILAPNLMWLNWSTLFIKNTSSKQVNNITVE
                     ICSETFQVASKPHSSSLRLLPKCGDATLILKANAKLVCQYYVEGELYNVEADVGSGKC
                     KTDLPIISSLFLLKLIK"
     gene            4336..4635
                     /locus_tag="AC159U_12005"
     CDS             4336..4635
                     /locus_tag="AC159U_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337818.1"
                     /translation="MKKYALNGLFAGIILLTIAVFVVIYIEWGKFDGRCIKMGFPFLA
                     EAQEYDCTFTEYIKDDSRSIFWLWPYDPRLWVAIISILVIPVVAGSILGFRKKST"
     gene            4679..4912
                     /locus_tag="AC159U_12010"
     CDS             4679..4912
                     /locus_tag="AC159U_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3337819.1"
                     /translation="MPISKEQLFKEAMDLDDNARANLAGLLIESLDTEAENGIENAWL
                     KEIEQRMESLDSGKTETVSWDTVKERLYNNVNG"
     gene            4905..5201
                     /locus_tag="AC159U_12015"
     CDS             4905..5201
                     /locus_tag="AC159U_12015"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3337820.1"
                     /translation="MADYEVRFHPEAIKEAESSQHWYVERSQTAALGFTNEISSAIEK
                     ILKSPTRWPKYQKNTRRYILPRFPFSVIYRIKDNIIEVIAIAHHKRKPGFWRNR"
     gene            5243..5581
                     /locus_tag="AC159U_12020"
     CDS             5243..5581
                     /locus_tag="AC159U_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337821.1"
                     /translation="MNREEYNEEDFLRQYIFNYYPHLFTKVEELSFKRILANEKAIHS
                     SPKMANILKKSWGSENNPKVDDALREGIEKFKQAVFARLMQEHKDEIFINRCPECNKI
                     VRTPKAKQRP"
     gene            5787..6215
                     /gene="rplM"
                     /locus_tag="AC159U_12025"
     CDS             5787..6215
                     /gene="rplM"
                     /locus_tag="AC159U_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3337822.1"
                     /translation="MKTYSARNTDLEKTWFVVDAADKNVGRIATKIATTLRGKDKPQF
                     TPHSDTGDFVIVLNAEKVNFSGKKWQQKNYYTHSHYPGGLKSMNAETMLEKKPTEIIR
                     KAVWGMLPKNKWQKKLIARLKIYTGTEHPHEAQTPQPLEV"
     gene            6219..6614
                     /gene="rpsI"
                     /locus_tag="AC159U_12030"
     CDS             6219..6614
                     /gene="rpsI"
                     /locus_tag="AC159U_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3337823.1"
                     /translation="MAVTEFYGTGRRKTSTARVFLRPGDGTITVNNREVNTHFPRKTW
                     QLKVLKPLELTGSTNKYNILMYVKGGGLTGQADACAHGLSRALVKADEANNKKPLKAA
                     DLLTRDPRMVESKKYGKRKARRGQQFSKR"
BASE COUNT         2362 a         1069 c         1118 g         2088 t
ORIGIN      
        1 aaaccagaaa agacccggca gacgtgacct gcatttcttg aatagggacg ggatggtcgc
       61 atgcaaccct cgtgaccacg aagctgcgca tagagctgag gttgagggga ttgctactga
      121 gaataaagaa gctttgacat gcaagaagtg tctagttgaa attagaaggg aacttgtaac
      181 taaataattg aacagtattt cattttaaat actataatgt atgcttactg atatgccccc
      241 attcttatta gtattggcaa aaatcatttc aagacaaatc aatattaact aggagctcaa
      301 tttgagtata tttgctatta tcttcacagc attatctttt ttgtttggat tgatttttct
      361 tatagattgc atcagatata gaaaacaaat taatgaattt gtgaaatatg gaatttgcct
      421 agggcttatt ttagtatcaa ttgatatagc aatatattat ttaatatcaa actatttatc
      481 tgatattcac attgttatat tagtattatc tgaatttcta agtttagtac aaataacaat
      541 ctttacatgt atgggaatgt atatttgtaa acagctaaat ttcaaggaca ttccctttgt
      601 aagaagtttt tttgaaaaat catataacat taatagaaac tatatatttc aaaaacgttt
      661 ccttgtttat gtgttttcag taatttatct ttctgtagct tattcaataa ttttatttag
      721 tctgacttca cctgagatgt cacaaacatt aaagcatatg tcagaatcac agttagaaga
      781 attacccaac ttaaacaaac cctcttttct agcagctctt gtagtattag agtttgcatt
      841 tgcagaggag ataatattta gacttggtat tcagaactac attcaaaaaa tgttaaattt
      901 aatagattca caatattgga tatctattag cttaacatct cttttttggt catttgctca
      961 cgcaaacgtg cttgatcctg aatgggtaaa aattgttcaa atattcccga taggtctggg
     1021 ccttggatat ttatttaata aattcggaac tgaagcatgc atactagcac atggtttttt
     1081 caatataata atgatgtttc taactcctta tttatttaca atttgaacgt attgttgatt
     1141 aattttggaa ataatataga agaatttaaa agatatttct catgaaatac atctataaat
     1201 cacttgattt ttcacaacga aatttgggat tgcttggtat atttatactt ataaatatct
     1261 ttcctgcccc tctgagctta ctttataata atggtgaaga atcaaatata tcaaatatgt
     1321 tcttaatcat attaattata gtatctctac tattatttat attaaatatt tttttgatat
     1381 caggaactta ttctttgatc tggaaaaaat ttaaggataa acacataagt tattcatcat
     1441 taatatctga atccaaaaaa tatttttcta ataatattgg tattggtctt gcgataggtt
     1501 tcactggatt tatagtggca ttatttatta ttgttatagt tggagtattt agtaaagaca
     1561 tagataatag accggactac acggatttct ctattatagt acctttatat ttttatctgc
     1621 tgctcacaat attttgttat gcaattcctt atctttatgt aaaagaaatg aattataaaa
     1681 atgcattttt caattctcca aagatactta ttaatcatat taagcaatct tttccaataa
     1741 ttttacttct ttttataaat ctagtcatat ctttttcgac atcatatgac caatcttatt
     1801 atttctctga tatcatcata gaaataataa tctattacat aagttttaat atttttataa
     1861 tcacatgtca gatattggat gaaatagata tctcggattt tgaaaatggc tcaacacaaa
     1921 acaggaaaat catttaatca ggttcagggt gtcagagcca atagatacag tggctcgaag
     1981 cttacaaact ggatggattt taatccagcc gggagttcct tatataaaat ttattctgat
     2041 cattacaatg agttcataaa caataaaaac aagatttcgt attttaaaca aaaaacacat
     2101 cctgatttca taatgcctct aaaaatttca ggaattaaaa aatatctcca gaatgtacca
     2161 gagaaatatt acaaggatat aaaagctata tttttattat caggttcaaa caagcaggaa
     2221 aaagtatttt tcagtaaact attttgctat ggcacttaca tggcaaactg tatctttatc
     2281 catccctatc caaaaaacaa tatgaatctg atatttaaaa accccccaaa accaaacatt
     2341 cttaatgact ataggagagt cggagcgctg atagaaaaag acgacacaaa gggaactatt
     2401 ttaaaatgga caaaagaatc acttaaagat ttctattata gggatgtact gatgcatgag
     2461 atcgggcacc actatgataa atatgtctgg gttaaatcaa acaaaaaagc agaaggattt
     2521 gcagagtggt ttgctacaga atatggattt aagtttaagc atttgaaaga ttagtctgtt
     2581 aaaaatattt tttatgtaca aatcccttat caccaaactc acaaaccaaa taaaccaaca
     2641 ctccaaccct gctgaaaaat cctggtttga aaactatata aaaaacagcg ctccgtttag
     2701 aggcgttaag atgccagtag ttagaaaaga acttcataaa tggcataagg attacattga
     2761 tggcgaaatg gatttagacc agcagcttga tttagcattt gctcttatag aagaaaaata
     2821 taccgaggac aaaattgccg ggactatgtt catgcaggaa attttgattc caaaaggaat
     2881 catgaagcca aaaagaaata ttaaaagact ttcagaactt ttcagcaatg gacacatcta
     2941 tgactggaat gtgtgcgact ggttttgtat gaaggtgctg ggtgagctaa tcgtgaaaga
     3001 tgagaaatac tgggcaagac caattcatat gtggcacagt gctgagaacc tctggcaggc
     3061 ccgcgcgtcc ttagtttcat ttgaaagggt ttccgataat caggattact tcagcatgat
     3121 tgaaaaatcc tgtaagaagc taatcaaaag agaagaacgt ttcgcaaaga ctgctgttgg
     3181 ctggcttctt agagaaatat caaagcagga tgaagatttt gtaaagcagt tcatcgaaca
     3241 tcacataaaa cattttacag ctgagacaat cagaaatgca ctaaaatatt catcgaaaga
     3301 tgagaagaaa cattatttaa ccttgctgaa atctgtttaa tattccaacc ctattcatgc
     3361 ctcagtatcc aaaaacacat caaaagtctc cagatgactc tcggttgaaa aagcctcttt
     3421 tggcagtgtg ccgctttttg catgaccttt aataaaagca tcagatttta cccagttatt
     3481 aaaatcatcc tcggattccc agaaagtcat caccacatat ggctgctcag gccccttggg
     3541 tttcaagacc acattgcgaa caaacccttt catcttatcc acttcatgtg cccggttttt
     3601 aaacctctgc tcaaatccat cggtatatcc ttcattcaca aaaattctgt tcatggttac
     3661 aaacattttt ttctcctttt tgtaccctta cataaaatca aagaatctac atattaatta
     3721 aactatctaa aataattgga tatactatta tataaataag ggtattcatt atgtttggaa
     3781 taaacttaaa agtaatttta acatcaatat caatatatat gggtatttat gccggaagtt
     3841 tgattgctga atttattatt tacggctaga aatttcttct tccataatgt tttcataacg
     3901 tttattccct cttctccata taatatacaa tcagctttgt aagcttttct aaactattta
     3961 tagtaaacta tcaattacta tgaaaaagtg gttagtagca cctttaataa tattcatact
     4021 gatactagcc cccaacttga tgtggcttaa ctggtctact ttatttataa aaaacacgag
     4081 cagcaaacag gtaaataata taaccgtaga aatatgttct gagacattcc aggttgcttc
     4141 taaaccccat agctcctctc tgcgtctcct tccaaaatgc ggtgatgcca cgctcattct
     4201 aaaagcaaat gccaaacttg tgtgtcaata ttatgtggaa ggcgaactgt ataatgtaga
     4261 agcagacgtt ggcagtggta agtgtaaaac cgaccttccc attatttcat ccctattttt
     4321 attaaaattg attaaatgaa aaaatatgca ctaaatggat tgtttgcagg aataatatta
     4381 ttaactattg cagtatttgt agtgatttat atagaatggg gaaaatttga cggcagatgc
     4441 atcaaaatgg gctttccgtt ccttgcagag gcacaggaat acgactgtac gtttactgag
     4501 tacataaaag atgattccag aagcatcttt tggctatggc catatgatcc aaggctatgg
     4561 gtagcaatca tatcaatatt ggtaattccg gttgtagctg gaagtatttt aggatttagg
     4621 aaaaaatcga catagaaaat tgatatatat cagttgattt atatgttatt atttgtttat
     4681 gcctatctct aaagaacaac tattcaaaga ggctatggat ttagatgata atgctcgggc
     4741 aaaccttgca ggacttctta ttgagagcct ggataccgaa gctgaaaatg gcattgagaa
     4801 tgcgtggtta aaagagattg aacaacgaat ggaatctctc gattctggaa aaacggaaac
     4861 tgtttcttgg gatactgtca aagaaagatt gtacaataat gtgaatggct gattacgagg
     4921 tcaggtttca tccagaagca attaaagaag ctgaatcatc tcagcattgg tatgtagaaa
     4981 gaagtcaaac agcagcactt ggatttacta atgaaatttc ttcagctatt gaaaaaatat
     5041 taaagtctcc tactcgctgg ccaaaatatc aaaaaaatac tcgcagatat atacttccga
     5101 ggtttccctt tagtgtaatt tatcgcatta aggataatat aatcgaagta atagccattg
     5161 cacatcataa aagaaaaccg gggttttgga gaaatagata aatatattta acctttttaa
     5221 aatcaaacca aaattaataa caatgaatag agaagaatac aacgaagaag attttctaag
     5281 acagtatatt tttaattatt acccgcattt atttacaaaa gtagaagaac ttagttttaa
     5341 aagaatatta gcaaatgaaa aagctatcca ttctagcccg aagatggcaa atatattaaa
     5401 aaagagttgg ggctctgaaa acaatcccaa agtagatgat gcattaaggg agggtattga
     5461 aaaatttaaa caagccgtat ttgctagatt aatgcaagaa cacaaagacg aaatttttat
     5521 caatcgctgt cctgaatgta ataaaatagt aaggacacca aaagctaagc aacgtccttg
     5581 atgctttcat gattggcatt agccataata ttaaatatat tcaaatgatc agaagtgatg
     5641 actgacttga aaattttata gatatatatg ttattattat atcaaaattt tatcgtagat
     5701 attaattgga ttagtattta atgtaacttc ttgactaaaa aactatattt ggtattttta
     5761 cgggtctttt aggtagggat atatcaatga aaacatatag cgcaagaaat acagatttag
     5821 aaaaaacatg gtttgtggtg gatgccgcag acaaaaacgt tggtaggatt gcaacaaaga
     5881 ttgcaaccac tctcagaggt aaggataaac cgcagtttac tcctcattca gacactggag
     5941 attttgtgat agtgttaaat gctgaaaaag taaacttctc tggtaaaaaa tggcaacaga
     6001 agaattatta tactcactct cattatccag gtggtctgaa atcaatgaat gccgagacta
     6061 tgcttgagaa aaagccaaca gaaatcataa gaaaagcagt atggggtatg cttccaaaaa
     6121 ataaatggca gaaaaagctc attgccagat taaaaatata taccggcaca gagcatccgc
     6181 atgaagcgca gactccccag cctttggagg tttaaaacat ggcagtaaca gaattttacg
     6241 gtacaggaag aagaaaaaca tccacagcca gggtattctt aagaccgggc gacggcacaa
     6301 ttacagttaa caaccgtgaa gttaacactc attttccaag aaaaacctgg cagttaaagg
     6361 ttttaaaacc tcttgagtta acaggctcta caaataaata caacatatta atgtacgtaa
     6421 aaggcggcgg actaacagga caggccgatg cctgtgctca tggtctttca agagcgcttg
     6481 ttaaagccga tgaagcgaac aataaaaaac cacttaaagc agctgatctt ctcacacgtg
     6541 atcctcgtat ggtagaaagc aagaagtatg gtaagagaaa agccagacgc ggacaacagt
     6601 tctcaaagag gtaataacaa gatcgctact tctaaac
//
LOCUS       DBNGSV010000342         2261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1032941, whole genome shotgun sequence.
ACCESSION   DBNGSV010000342 DBNGSV010000000
VERSION     DBNGSV010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1032941"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..373
                     /locus_tag="AC159U_12035"
     CDS             140..373
                     /locus_tag="AC159U_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR3337824.1"
                     /translation="MKTVQMTLDEDLVNKVDKLVKKLDTTRSEFTRNALREAIRKYNI
                     SKLEDKHTKGYKNHPISKDEFEIWENEQVWGDE"
     gene            370..681
                     /locus_tag="AC159U_12040"
     CDS             370..681
                     /locus_tag="AC159U_12040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3337825.1"
                     /translation="MKKGEIRWYKFKPPDKKRPVLILTRNSIIDHLNEVTIAPITSTI
                     RDIPTEILLTKADGLKNDCAINFDHIQTVSKNKIGSVITNLSDAKQEQIKNAVMFAFD
                     L"
     gene            739..1116
                     /locus_tag="AC159U_12045"
     CDS             739..1116
                     /locus_tag="AC159U_12045"
                     /inference="COORDINATES: protein motif:HMM:NF044559.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6869 domain-containing protein"
                     /protein_id="HIR3337826.1"
                     /translation="MTELVIDKLANAYVNYYKSKKHSTKNPNWWAVEKFMHVGTEELS
                     AADCLSAILNILSKNPSDKVIAILAAGPLEDLIHECGEQVIDDIEKLARQNPKFRHLL
                     GGVWESSTPEIWSRVLACRSEVW"
     gene            1120..1656
                     /locus_tag="AC159U_12050"
     CDS             1120..1656
                     /locus_tag="AC159U_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337827.1"
                     /translation="MLISEFGENTTGTIDEAQSTFVKFTDSKSGYTFKIEYRDKQEYY
                     IKPGSFRGISIVNKHPLLEQHEYSYTNLYISSASKEYEIVDTKIEDAIYEHYNGWRGI
                     KNYCNISYGVEKILKQGSGLLYEGPPSGAEVVNNVLKEYNILYSSTSEGKPNGQKYKV
                     LFLGSNFVVARDFEVVLL"
     gene            1668..2060
                     /locus_tag="AC159U_12055"
     CDS             1668..2060
                     /locus_tag="AC159U_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337828.1"
                     /translation="MKQELIYVELKSGHSDNGPAWIGYKGSSKSGATIYFNGMAFKSL
                     KGTGIGANYYECETGDQYWISGVKKNGSDRHWAGNGDILIDKNAVKDYLDIRGLRALP
                     KTLVPIELKPSVPSLDFLENENMVKDFE"
     gene            2061..>2261
                     /locus_tag="AC159U_12060"
     CDS             2061..>2261
                     /locus_tag="AC159U_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337829.1"
                     /translation="MKPPIGKVLLFLSTDSGDPSDCISWAEEALHNGFDSPSLRILAG
                     LDKPLNSFEVNEYTTRALHELNI"
BASE COUNT          815 a          354 c          457 g          635 t
ORIGIN      
        1 ctgctcaccc gatgcagcct ggatcactgg ttcagttttc acaatggacg gcggggctat
       61 ggcggaatag gtatatacta gactttgcat cctatgtgta ttataatagc tatatatatc
      121 cacattagag gtgctttaca tgaaaacagt acaaatgact cttgatgaag acttagttaa
      181 taaagtagat aagttggtta aaaaacttga taccaccagg tccgaattca caagaaatgc
      241 tctgagagaa gctataagga aatataatat ttccaagctt gaagataaac atactaaggg
      301 ttataaaaat catcctatca gcaaagatga atttgagatt tgggaaaatg agcaggtatg
      361 gggcgacgag tgaaaaaagg tgaaataagg tggtacaaat tcaaaccacc agataaaaaa
      421 agacctgtat taattcttac tcgaaattcc attatagatc acctaaatga agttacaata
      481 gcacctatca catcaactat aagagacata ccaacagaaa tattgcttac taaagctgat
      541 ggccttaaaa atgactgtgc aataaatttt gaccacatcc aaacggtttc taaaaataag
      601 ataggatcag tcataacaaa cctttctgat gcaaaacaag aacaaatcaa aaatgccgta
      661 atgtttgctt ttgatttgta ggtacaataa aaagaataga cggtggggct atggcggagt
      721 gatgtaattt ttcagataat gacagaactt gtaattgata aactagccaa tgcatatgta
      781 aattactata agtctaaaaa acattccacc aagaacccta attggtgggc tgttgaaaag
      841 tttatgcatg ttggaactga agaactatcc gcagctgact gtctatcagc tatattgaat
      901 atattatcaa aaaatccatc cgataaagta attgcaatac tagcggcagg tcctctggaa
      961 gatctaattc acgagtgtgg ggagcaggtt atagatgata tagaaaaact tgccagacaa
     1021 aatccgaaat tcaggcatct tcttggaggt gtttgggaaa gcagcacacc tgagatatgg
     1081 tcaagagttt tagcctgtcg tagtgaagtg tggtgattta tgcttatttc agagtttgga
     1141 gaaaatacaa ctggtactat agatgaagca caatcaacat ttgttaaatt cacagattca
     1201 aagtctgggt acacgttcaa aatagaatac agagataaac aagagtacta tatcaaaccc
     1261 ggatcatttc gtggtatttc aatagtcaat aaacatcctt tacttgaaca gcatgaatac
     1321 tcttacacaa atctatatat ttccagtgca tctaaagagt acgaaattgt tgatactaaa
     1381 attgaggatg caatatatga acactacaat ggatggagag gaattaaaaa ctactgcaat
     1441 atatcatatg gagtggagaa gatattaaaa caaggatcag gtttattgta tgagggaccg
     1501 ccatcaggag cagaagttgt aaataatgta ttaaaagaat acaatatttt atacagctca
     1561 accagtgaag gtaagccgaa cggtcaaaaa tataaggttt tgtttttagg cagtaacttt
     1621 gtagttgctc gtgattttga agttgtttta ttataataat tgatcatatg aaacaagaat
     1681 taatatatgt tgaattaaaa tctggacata gtgacaacgg ccctgcatgg ataggatata
     1741 aaggttcatc aaaatcaggt gcaactattt attttaatgg aatggcattc aagagtctta
     1801 agggcactgg tattggcgcc aattattacg aatgcgagac tggtgatcaa tattggatat
     1861 ccggcgtgaa aaagaatggg agtgatcgcc actgggctgg caatggcgat atacttattg
     1921 ataagaatgc tgttaaagac tacctagata ttagaggtct tcgagcttta ccgaaaactt
     1981 tggttccaat tgaacttaag ccctccgtac caagcttaga ttttctagaa aatgaaaata
     2041 tggtaaaaga ttttgagtaa atgaaaccgc caatcggaaa agttttactt tttcttagca
     2101 ctgattcagg agatccgtca gattgtatta gctgggcaga ggaagcttta cataatggat
     2161 tcgacagccc aagcctgaga atattagcag gcttggataa gcctctaaat tcatttgaag
     2221 tcaatgaata tacaacacga gcactacatg agctgaatat t
//
LOCUS       DBNGSV010000343         5425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_895338, whole genome shotgun sequence.
ACCESSION   DBNGSV010000343 DBNGSV010000000
VERSION     DBNGSV010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_895338"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2589
                     /locus_tag="AC159U_12065"
     CDS             <1..2589
                     /locus_tag="AC159U_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3337830.1"
                     /translation="SQIEKSIGRQVGVNKIRLNILGGLGLGLSGLSIKDDPSFFAGNF
                     LETKNLVVNVKLLPLLSKEVQVKKIILNDPVIRIIKNKNGDFNFSTIGGKENPNQTES
                     EKESTVKKFNISLVDVSNGNIQYIDREQRSTIELDNIDLSLKNIGLDEKIDIDLALSL
                     LDKNQNIKIKGNIGPLGQQINTDSLPLSLDLQISSLNIKALKSSIPQLKKSLPPDINQ
                     IDKLSLKTRIDGDLSNMRLTDLELESHLFGSTQSNLFVNGSVGPVGAKVSEHDMFFKL
                     NMDIGPLNSQQLLSIDSLSTLVPADLKMNGPISFMADIEGNPSNITIKNGILEASSTD
                     IVYAESFNKPKGIDLTLKTDASLNKDNMKLENTNLKLDSLQLDIYGTYNTSSTNADIN
                     IKSNKADLQKLSSLLPDLSTHNISGFVEADAKIKGLTGQGKTPAINGFIKLTGVNAKP
                     EQLAKPINDLNSEIKFTGNSASLDKTDLVIGKSKISVISEAASISPISVSYVIASPKI
                     YMADVSPDNKTDESINDVTITGKVFEQVGTMIHRATIKSRSGKLSSLGYSNLNGKLSI
                     KDDVITLDQMAMNILSATINANGVYDLSNPKPAFDLKTQIKGLSITNLTQSLFRADNQ
                     HLRGNSDLNIDISGSGNTWDEIKPTLSGLTKISLTEGELVDFNIAEGVLSGITGVSGL
                     SGLVSSSLKSKYPQVFQSKSTIFYDLKSVLKIDDGKINFNDLILKATDYLVKGDGSIG
                     LDKGIDVNGQLKMSEKFSNDLIANADFIKYLRNDNNEILIPFDLKGLLPNVSAKPDLT
                     FVANKLKGAAIDRGRDELQKRVIDKIIPKTTSPDTESEGGDQQTKPKSIEEDLIQKGL
                     DKVLDF"
     gene            2795..3013
                     /locus_tag="AC159U_12070"
     CDS             2795..3013
                     /locus_tag="AC159U_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337831.1"
                     /translation="MKRKYARMMTRDIPNLGMQYMIDEIRNISDDEARLINKDIEHVQ
                     SIWVPIPDGLFDKWVDELYIGDKEALKQ"
     gene            3062..3658
                     /locus_tag="AC159U_12075"
     CDS             3062..3658
                     /locus_tag="AC159U_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HIR3337832.1"
                     /translation="MSDKERYQKAISLIDKANLQDPNTELLDGKEFPKELLYSNRMTS
                     WLDILDPEASDVLKIAARAQHIERWKIPRSKYPVDRDGYRMWRKELMDFHASRVSEIM
                     SHVGYDEDSILRVKSLIKKELFKTDPDSQMLEDVVCLVFLENYFSDFSKEHLNDPDKL
                     SRIITKTWNKMSENGRELALKLKIPAEAKSLISKALQG"
     gene            3728..4459
                     /locus_tag="AC159U_12080"
     CDS             3728..4459
                     /locus_tag="AC159U_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337833.1"
                     /translation="MSKVNELLDKVIEAHGGMDAWNRVDEINVTARTGGLLPLSKLKY
                     SSLSKVNFTVYPKTMMTVFNDFPELGSKGVFDKGTVYIENGSGEIEEERENPREQFNR
                     ISKKLYWDYLDTIYFTGYAIWNYLCTPFMFKTYDFELSIGQSIEDEGEILDALKVIYP
                     DNIHTHCKEQTLYFDSFGLLRRLDYTADVIGSWAHAAHYCFDYVKTDGISFPLSRKVY
                     MRKTDGTHAEFPIVIWIKLSQINIK"
     gene            4466..4714
                     /locus_tag="AC159U_12085"
     CDS             4466..4714
                     /locus_tag="AC159U_12085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3337834.1"
                     /translation="MGLEHLKSIPLLNDVYFKIWLKDAASKINRIFTHLGQEDKILDI
                     GCGPGSVSLLLKEEGYDVTALDIKDLTYTDRINPVLYN"
     gene            4721..5050
                     /locus_tag="AC159U_12090"
     CDS             4721..5050
                     /locus_tag="AC159U_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3337835.1"
                     /translation="MPFADKQFDISLLLRVLHHVSDPINLLKEAARVSKKIIIIEDVY
                     NSTIQQCLTYIVDSLVNFEISGHPHTNNTDIQWRDQFDKHGLKLSTASVDNYIYLFRQ
                     AVYVLNT"
     gene            5096..>5425
                     /locus_tag="AC159U_12095"
     CDS             5096..>5425
                     /locus_tag="AC159U_12095"
                     /inference="COORDINATES: protein motif:HMM:NF017321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337836.1"
                     /translation="MSSQYNKKKSEAPLFRPPKTSIPLAERVRPKKLSEVIGQDHLLG
                     KNGVISEMVKSGNLSPMIFWGPPGTGKTTISRLLADEINAGFVQINAISSGVKELREI
                     IKEAEDSF"
BASE COUNT         1935 a          860 c         1056 g         1574 t
ORIGIN      
        1 tctcagatag aaaagtctat aggaaggcag gttggtgtta ataagatacg gttaaatatc
       61 cttggaggtc tggggcttgg tctgtcaggg ctaagcatta aggacgatcc aagctttttt
      121 gcaggaaact ttttagagac taagaacctg gtagtaaatg taaaattact ccctttgtta
      181 tcgaaagaag tccaggttaa aaagatcata ttgaatgatc cggttataag aataatcaag
      241 aataaaaacg gagatttcaa tttttcaaca atcgggggta aagagaatcc caatcaaact
      301 gaaagtgaaa aagaaagcac agtaaagaaa tttaatattt ctcttgttga tgtatcaaac
      361 ggcaatatac agtatataga cagagagcag agatcgacta ttgaattaga taatatcgat
      421 ctttctttaa aaaatatcgg actcgatgaa aagatcgata ttgatctagc actttcattg
      481 cttgataaaa atcaaaatat taaaataaaa ggtaatattg gcccgttggg acagcagata
      541 aatacagatt ctttaccttt aagtcttgat ctgcaaataa gctcattaaa tataaaagcg
      601 ttaaaaagct ctatacctca gttaaaaaag tctcttcccc ctgatataaa tcaaattgat
      661 aagctaagtc tcaaaaccag aattgatggt gatctgtcaa atatgagact cactgacctt
      721 gaacttgaat cccatctatt tggttcaaca caatccaatc tctttgttaa tggaagtgtc
      781 ggacctgttg gggctaaagt ttctgagcat gatatgtttt tcaaacttaa catggatata
      841 ggacctctaa actctcagca gcttttgagc atagattcat taagcacatt agtgcctgct
      901 gatttaaaga tgaacgggcc tatttcattt atggccgata tagagggaaa tccttcaaat
      961 atcacgatca aaaacggtat tcttgaggca agcagtacag atattgttta tgcagagagt
     1021 tttaataaac caaaaggaat agatctaact ctaaaaactg atgcaagttt aaacaaagac
     1081 aatatgaaac ttgaaaatac aaatctaaaa ctggacagtc tccaattaga tatatacgga
     1141 acatacaata ctagtagtac aaatgcagat ataaacatta aatccaataa agcagacctg
     1201 caaaagctat caagtctcct gcctgacctt tctacacata acatatccgg ctttgtggag
     1261 gcagatgcaa agatcaaagg acttacggga cagggtaaga ccccggctat aaacggtttt
     1321 attaaactta caggtgtaaa tgcaaaacct gagcagcttg caaaaccaat aaatgatctt
     1381 aacagtgaga taaaatttac aggtaacagc gctagtttgg ataaaactga tctggtaata
     1441 ggaaaatcta aaatcagtgt tatatcagag gctgcaagca tttctccgat atcagtatcc
     1501 tatgtaatag cctcaccaaa aatttatatg gcagatgtaa gtcctgacaa taagactgat
     1561 gaatctataa atgatgtaac gattacagga aaagtgttcg aacaggtggg aacaatgatt
     1621 cacagggcca ccataaagtc ccgtagtggt aaattgtcga gccttggata cagcaatcta
     1681 aatggaaagt tgtcaataaa agatgatgta attacacttg atcagatggc aatgaacatt
     1741 ctaagtgcaa ccataaacgc aaacggagtt tatgatttat ctaatccaaa gccggcattt
     1801 gatttaaaaa cacagattaa ggggcttagc attacaaact taactcagtc actattcagg
     1861 gccgataacc aacatttaag aggtaactct gatttaaata ttgatatatc cggaagcggt
     1921 aatacatggg atgaaattaa gcccacattg agcggcctta cgaaaattag tcttacagaa
     1981 ggggaattgg tagattttaa tattgctgag ggtgtgctgt ctggtatcac gggagtaagc
     2041 ggtctttcgg ggcttgtaag cagttcatta aaatcaaaat acccgcaggt attccaaagc
     2101 aagagtacga tattctatga cttgaaatct gttcttaaaa ttgatgacgg aaagattaat
     2161 tttaatgacc tgatcttaaa agccactgat tatttggtaa agggtgacgg ctctatagga
     2221 ctggataagg ggattgatgt aaatggacag ttgaaaatgt ctgaaaaatt ttcaaatgat
     2281 ctaattgcta acgccgattt tattaaatat ctaagaaacg ataataatga gatccttata
     2341 ccttttgatc ttaaaggtct tctgccaaac gtaagcgcca aacctgatct gacatttgtg
     2401 gccaataagc taaagggtgc tgcaattgac aggggtaggg atgaacttca aaaaagagta
     2461 atagataaga taattcctaa aactaccagc ccggatactg aatctgaagg tggtgatcaa
     2521 caaactaaac caaagagcat cgaagaagac ctcatacaaa aggggttaga taaggtatta
     2581 gatttttaaa ttatattaaa tatcttaaat aactacttga actattagtc ataatatatt
     2641 ataattattg caaatataga gattttgggg aggatgatga aatattaaag gccgaaatct
     2701 ctatattaaa tacattagag caaatggcat gaatattgct taattaatat ttgaatgaag
     2761 gagagtattt attctcttgc cggaggatga tgaaatgaag agaaaatatg ctaggatgat
     2821 gacaagggat attcctaatc tgggaatgca gtatatgatt gatgaaataa ggaatatatc
     2881 tgatgacgaa gccaggctta tcaataaaga tatagaacat gttcagtcta tatgggttcc
     2941 tattcccgat ggattgttcg ataagtgggt ggatgagcta tatatcggag acaaagaagc
     3001 tctcaaacaa taaaatcata ttaagactta atatttaaag ttattatatt aaactttctt
     3061 gatgagtgat aaagaaagat atcaaaaagc tatatccctt atagataagg ccaatctgca
     3121 agatcctaac acagaacttc tagacggcaa agaatttcca aaagagctgt tatattccaa
     3181 cagaatgaca agctggctcg atatacttga cccggaggca tcggatgttc tcaaaatagc
     3241 agctcgtgca cagcatatag agcgttggaa gattccacga tcgaaatacc cggtggatag
     3301 agacgggtat agaatgtgga gaaaagagct tatggatttt catgcatcga gggtttcaga
     3361 gatcatgtct catgttggtt atgatgaaga ttcaatacta agagtaaaat cactgattaa
     3421 aaaagagtta tttaagactg atcccgactc acagatgctt gaagacgtag tatgccttgt
     3481 gttcttggaa aactactttt ctgatttttc aaaagaacat ctaaatgatc ctgataaact
     3541 aagcagaatt attactaaga cctggaataa gatgtctgaa aatggcagag aacttgctct
     3601 caagcttaag atacccgcag aagctaaatc tcttatatcc aaagctctcc aagggtaata
     3661 ttatttaagt tattttatct tttaattcga attgaacaat atcgctggta tattgtgatt
     3721 aagagctatg agtaaagtaa atgaattatt agataaagta atagaagccc acggcggtat
     3781 ggacgcatgg aacagggttg acgagataaa tgtaaccgca agaacaggcg gtcttcttcc
     3841 tctatccaaa cttaagtatt catcactgag taaagtgaat tttacggttt atcctaaaac
     3901 aatgatgact gtatttaatg attttcctga gcttggaagt aaaggagtat ttgataaagg
     3961 cacggtatat atagaaaatg gatctggtga gattgaagaa gagagagaaa atccaagaga
     4021 gcaatttaac cgaataagca agaagcttta ctgggattat cttgatacta tatattttac
     4081 aggttatgca atatggaact atttgtgcac tccttttatg tttaagacat atgattttga
     4141 attatctatt ggacaaagca tagaggatga aggagaaatc ctcgatgctc taaaagtaat
     4201 atatcctgac aatattcaca cccactgcaa agagcaaact ctttactttg acagtttcgg
     4261 acttcttaga agactcgatt acacagctga tgtaatagga agctgggctc atgctgcgca
     4321 ttactgtttt gattatgtaa aaacagacgg aatatctttt ccactttcaa gaaaagtata
     4381 tatgagaaaa actgacggta ctcatgctga atttccaatt gtaatctgga taaaattatc
     4441 acagattaat attaagtaat accctgtggg tttagaacac ttaaaatcca tccccctgtt
     4501 aaatgatgtt tattttaaaa tctggttaaa ggatgctgca tctaaaataa atcgaatctt
     4561 cacacattta ggacaagagg ataaaatttt ggatatagga tgcggccccg gtagtgtttc
     4621 actgttgtta aaagaagagg gttatgatgt tactgctctt gatattaagg atttgaccta
     4681 tacagataga ataaaccctg tgctttacaa ttgaaggact attccgtttg ctgataagca
     4741 atttgatatt tcattacttc ttagagtttt gcaccatgtt agcgatccca taaatttatt
     4801 aaaagaagcg gcaagggttt caaagaaaat tattattatt gaagatgttt ataacagtac
     4861 tattcagcaa tgtctaacct atatagtgga tagtctcgta aactttgaaa tatcaggcca
     4921 tcctcataca aataacactg atatacagtg gagggatcaa tttgataaac atggactgaa
     4981 attgtccact gcatctgttg ataattatat atatttattc aggcaggctg tttatgtgct
     5041 taatacataa ctaatcattt gatttattgt aagttacaaa ctagtcttaa tatatatgtc
     5101 ttctcaatac aataagaaaa aaagtgaagc gccgcttttt agacctccca aaacttctat
     5161 acctcttgcc gagagagtaa gacctaaaaa actttctgag gttattggcc aggatcacct
     5221 actcggaaaa aacggagtaa tctctgagat ggttaaaagc ggcaatctct cacccatgat
     5281 cttctggggt cctccgggta ctggaaagac tacaatctca agactgctgg ctgatgagat
     5341 aaacgccggt tttgtgcaga taaatgctat ttcttcagga gttaaagaac tacgagaaat
     5401 aataaaagag gccgaggact cattt
//
LOCUS       DBNGSV010000344         8787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1933985, whole genome shotgun sequence.
ACCESSION   DBNGSV010000344 DBNGSV010000000
VERSION     DBNGSV010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1933985"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1296)
                     /locus_tag="AC159U_12100"
     CDS             complement(88..1296)
                     /locus_tag="AC159U_12100"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3337837.1"
                     /translation="MTVKRKIVLAYSGGLDTSIILRWLIEQYDADVIAYVADVGQGED
                     LKAAKDKALKTGASKVYVEDLKKEFVKEFVFTSIKADAVYEGEYLLGTSLARPIIAKR
                     QIEIARKEKAFAVSHGATGKGNDQVRFELTFYSLDPKIKVIAPWREWDMKSRTDLINF
                     ARKHKIPVPVSKAKPYSCDRNIFHTSYEGGILEDPWTEPPESMFELTVSPEKAPDKAK
                     YLDIEFKTGEPVKIDGKRFKPVELLSELNKIAGKNGVGRVDIVENRYVGMKSRGVYET
                     PGGTLLLAAHRAVESLTLDREVMHLRDSLISKFSELVYYGYWYTPEMEMLRAAIDESQ
                     KNVNGTARLKIYKGNVIVVGRKSPNSLYDEELATFEEDTVYDQSDATGFIKLNALRLA
                     VRELSKKGKR"
     gene            complement(1346..1780)
                     /locus_tag="AC159U_12105"
     CDS             complement(1346..1780)
                     /locus_tag="AC159U_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337838.1"
                     /translation="MLTLMSWVESEDYWNINRLDNEGRQLDYYAYRFEVSGGSESQGV
                     SKVSVVVVELISAKIAVGYVLPDHIEIKSEFKIGFICQDKPTEDISFVCKLSEEVKKA
                     NYNGDDLEKLEYIGFSLEKFYENQGAKFYMQDLRGAPATAEK"
     gene            complement(1796..2467)
                     /gene="pgeF"
                     /locus_tag="AC159U_12110"
     CDS             complement(1796..2467)
                     /gene="pgeF"
                     /locus_tag="AC159U_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR3337839.1"
                     /translation="MSNKELNIEVSDKLSQFDNIIEYGFCNKSTQLGFDHNIVTLKQI
                     HSKQVHRIDHNTDYTNLQGDALITDVPGVRIGIYTADCVPVLLYDISKSVIGAVHAGW
                     RGTFSDIISETVTAMKSVYNSDPEDIISVIGPCISICCFEVDGDVAIKFIEKYEETDS
                     FIYPNGEKYMIDLAQINKQSLKHAGLSNIEVIDICTKCDLGYHSYRREGAGVGRQLSF
                     IGIKI"
     gene            complement(2460..3410)
                     /locus_tag="AC159U_12115"
     CDS             complement(2460..3410)
                     /locus_tag="AC159U_12115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3337840.1"
                     /translation="MGKQLSYDEIIIPKSFAGKRADTTISDLLPSITRVQIKRLIDEH
                     HILINELPFKPSRKLSGGETVRIEHVEPEEIDIVPQDIEIDFIYEDRDIVVINKPPGI
                     SVHPGAGIKSGTLVNALLYKCKNLSGIGGKIRPGIVHRLDKDTSGIMVVAKNNLSHNH
                     LVEQFKGRTIKKKYLALLIGNPKEQTGEITLPIGRHRTNRIKFSSDSNSTKTALTKWS
                     VVKRFKGACLCEAEPYTGRTHQIRVHFSEIGYPVLGDKLYGKKIQDAVINNISKNLGR
                     QALHAFSLSFEHPRDGKSLYFEAQIPHDMKEAIRELEGYV"
     gene            complement(3419..4543)
                     /locus_tag="AC159U_12120"
     CDS             complement(3419..4543)
                     /locus_tag="AC159U_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337841.1"
                     /translation="MEYNLESIPENLRRFIDPSLDRDTRIMAARGLVPIPPEDMALVL
                     YYLTFDKDDEVVNESKQSLFTLPYNTLERVLSNESVPDGLLDYFAKNTENDIHFEKII
                     INNATSDSTIQYLAENIHNQYLIELISDNHQRILRSHEIVYALSKNPAISRSNLDKVI
                     SFISLYLEKEVNIPQALQDSSEEPQQVDQSEAIVESVESSFLDEVEIPEEFLEEEGEE
                     GELDLEEEQSPKYQNLFFAIKSMTLPEKLKLCIMGNAEARRILVKEPNRVVALAALKN
                     PRMTDMEINIAAQSTSVSEEVLREISNNRDWTKHYDIKVSLVTNPKSPADVSMNFIRH
                     MRDKDLKLLSRSKNVPGVVSTAARRILMQKEESQKLKLQS"
     gene            5040..5786
                     /locus_tag="AC159U_12125"
     CDS             5040..5786
                     /locus_tag="AC159U_12125"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3337842.1"
                     /translation="MKITILGSGTCIPSINRGSSGYFFDTGKNKILFDCGNGITWKLE
                     RLGESYTDITHIFISHIHPDHTADLIPFLFANKYPYNRKRTRKLEIWGPPGFMDFYTA
                     INKIYNDWLKPEFLEVYEMEQAIYEFNDFTIINKPGNHSTDSLIYRIECGGKSVVYSA
                     DTDYCKNLAVLSKQTDLLIAECSMKDDKKVKGHMSPKDIIKIANEANPKNIVLTHLYP
                     ICDEVDIIGQIKPHIESDIIKAHDMMELNL"
     gene            complement(5811..6962)
                     /locus_tag="AC159U_12130"
     CDS             complement(5811..6962)
                     /locus_tag="AC159U_12130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3337843.1"
                     /translation="MKYKIQILKKIDAIIGTPAVHLSRLIRPRKSEFYSLDDLQSPVS
                     KNFDKLKILVIRPGGIGDAVLLFPALKILKDIYANCNIDILAEKRNQGIFENCEFINE
                     LILYDNDPIKALKKVFSNNYEIVIDTEQWHRLTSVVACLTRAPVRVGYGTNERRRMFT
                     NVVQYSHDDYESQSFLNLVSGISKREYEFDKNSPFVPVDDSRCSDLNKQLIEYSSKWD
                     WVAGIFCGATVSERIWGVDKFIKTTAKLLESNIGVVILGAKSELGDCIRIERSVGKDN
                     ILNLTNKTSLYQTAVVISKLNLFVSADTGLMHIAYGVGTPTVSLFGAGIEKKWGPKGD
                     DNININKNLPCSPCTSFGYTPSCPIGVKCLQDISVDEVVNSSKKLLYAN"
     gene            complement(6928..8688)
                     /locus_tag="AC159U_12135"
     CDS             complement(6928..8688)
                     /locus_tag="AC159U_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337844.1"
                     /translation="MTKYKRGIYYSLVLCVISFVLYLPSLDNKLVWDDSDAINKKHYK
                     YQGFINLLTPQQKSKKGNYYRPTVYLSYFIDQTLWGNKPFGYHLSNLIFNTVTVILLF
                     WFVYFFFSNFKPNYSSYNIAFISSLVFALHPMHVESVSWIAGRTDVLCTLFLLMGLLF
                     HLKSYKKVVYIPFAILAFYLSLLSKELAVVFIPLVLLTDLVTNQLKNKNNAVKYILYL
                     SIFILYMYLRSKSFVNITPISEKNVNVAASADILRYLDMFVVLVSSYYSYLYKLLFPI
                     EFNAFISEVTKDLYYLVPSFIVLTLVFLAFIYSLIKRVKLIYYVIFWILISLGPSILL
                     TLIKMTPTSLAERYLYLPSVAFAMGTGYLFYKYVFSRVSLRYSCVFITILVLFYALLN
                     INRQSVWYDDLSLWSTTTVASSNTCLPYNNYGVALKRNGRIDEAIRQYEYVVNNKNIK
                     CRDAVRALVYNNLGTAYIKIDQQSKGRETFKKAMELNEYYHSPHLNLGIDYLLTALET
                     KSISEFEYAKQYFNNSISIRPNSGISHHYLSIAYKETGDLKNARIHASRALELGIPPA
                     QKNDALRILDEIQNSDSKEN"
BASE COUNT         2768 a         1718 c         1403 g         2898 t
ORIGIN      
        1 aggcaatata ttccgggggc aattcataat caaattcgga tgttttcatt tagttagaaa
       61 caaactatag gccatgtctt ctgcagctta tcgttttccc ttctttgaaa gttccctaac
      121 tgcaagcctg agagcattta gtttgataaa tccggttgca tccgactgat cgtagactgt
      181 atcttcttca aaagttgcaa gctcttcatc ataaagagag ttaggtgatt ttctgccaac
      241 gactattaca ttgcctttat aaattttgag tcttgctgta ccatttacat ttttctgaga
      301 ctcatcgata gctgccctta acatttccat ttccggagta taccagtagc catagtaaac
      361 cagttcagaa aattttgaga ttagagaatc ccttaaatgc ataacctctc tatcaagcgt
      421 tagggattcg acggctctgt gtgctgccaa gagaagtgtc cctcctggtg tctcatatac
      481 tcctcttgac ttcattccca catatctatt ttctacaata tccactctcc ctactccgtt
      541 cttgccggca attttattta attcagaaag taattctaca ggcttaaatc ttttaccatc
      601 aatttttacg ggctcaccgg ttttaaattc aatgtccaga tatttagctt tatcgggagc
      661 tttctcaggc gagacagtta actcaaacat actctcagga ggctcagtcc atggatcttc
      721 caatattcca ccttcgtagc ttgtatgaaa gatgttcctg tcacagctat atggctttgc
      781 cttggaaact ggcacaggga tcttatgttt tcttgcaaaa ttaattaggt ctgtacggga
      841 tttcatatcc cattctctcc agggagctat cacttttatt ttagggtcaa gcgaataaaa
      901 ggtaagctca aaccgaacct gatcattgcc cttaccggta gcaccatgtg aaaccgcaaa
      961 agctttttct ttcctggcaa tctcgatctg cctctttgct ataatagggc gggcaagcga
     1021 ggttccaagc agatattctc cttcataaac agcatcggcc ttaatggaag taaatacaaa
     1081 ctctttaaca aactcttttt tcaggtcttc tacataaact tttgatgcac ctgtttttag
     1141 agctttatcc tttgcagcct taaggtcttc accctgacca acatcagcta cgtatgcaat
     1201 cacatcggca tcatattgtt caatgagcca tcttaggatt attgaggtat caagaccacc
     1261 cgaatatgct agaactattt ttcttttcac agttatatcc ttggattatc agtattagat
     1321 aaataaatta ttaaaagtaa aaatattatt tttcagctgt agcaggcgct cctcttaaat
     1381 cctgcatata aaatttggca ccctggtttt catagaactt ttcaagagaa aacccaatgt
     1441 attctaattt ttccaaatca tcgccgttat aattggcttt tttaacttct tcagaaagtt
     1501 tacaaacaaa agatatatct tctgtaggtt tatcctgaca aataaaacct attttaaatt
     1561 cagattttat ttcaatatga tctggaagta catagcccac agcaatcttt gcgcttatta
     1621 attcaaccac tactacactt actttactaa caccctgaga ttcactcccc cctgagactt
     1681 caaatctgta ggcgtaataa tcaagctgcc gtccttcatt atccagtctg ttaatattcc
     1741 agtaatcttc agattccacc cagctcatta atgttaacat ccatgttcct cctaattata
     1801 tcttaatacc tataaaacta agctgccttc ctacccctgc cccttccctt ctataagagt
     1861 gataacccaa atcacactta gtacaaatat caataacttc tatgttgctc agtccggcgt
     1921 gtttgaggct ttgtttattg atttgcgcca agtctatcat atatttttca ccgtttgggt
     1981 aaataaaact atcagtttct tcatattttt caatgaattt tatagcaaca tcaccatcaa
     2041 cctcaaaaca gcatatacta atgcacggcc caattactga aataatatct tcaggatcac
     2101 tgttatagac gcttttcata gcagtaaccg tctcagaaat tatatcgcta aatgtacctc
     2161 tccaccctgc atgtacagct ccaattactg atttggatat gtcatacaac aaaacaggaa
     2221 cacaatcggc agtataaata ccaatacgaa ctccagggac atcggtaata agcgcatcgc
     2281 cttgcaaatt ggtataatcg gtattgtgat caatccggtg cacttgctta ctatgaatct
     2341 gttttaaagt aactatatta tgatcaaatc ccagttgagt gcttttattg caaaaaccat
     2401 attcaataat attatcaaat tgagatagtt tgtctgaaac ctcaatattc aattccttat
     2461 tagacataac cctctaactc tcttatagcc tctttcatat catgaggtat ttgcgcttca
     2521 aaataaagag atttaccatc tcttggatgt tcaaatgata gtgaaaatgc atgaagagcc
     2581 tgcctgccca ggtttttaga aatattattt ataaccgcat cctgtatttt tttaccataa
     2641 agcttatcgc ccaatacagg atagcctatc tctgaaaaat gaacccttat ctgatgagtc
     2701 ctccctgtgt aaggttcagc ctcgcaaaga cacgccccct taaatctttt taccacagac
     2761 catttggtaa gcgcagtttt agtgctgttt gaatctgatg agaattttat cctgtttgtc
     2821 ctgtgccttc caattggaag cgttatttct ccggtttgct cttttggatt gccaattaga
     2881 agcgctaaat atttcttctt aatagttctg cccttaaact gctccaccag atggttatga
     2941 gacaaattat tcttggcaac caccattatt cctgaggtat ccttatcaag acggtgcaca
     3001 atccccggtc ttatcttccc tccaattccc gagaggtttt tgcatttata cagaagcgca
     3061 tttacaagcg tgccactttt tattccggct cccgggtgaa cactaatgcc cggtggtttg
     3121 ttaataacta ctatatctct atcttcgtat ataaaatcaa tttcaatatc ttgaggtact
     3181 atatctattt cttctggttc tacatgctct attcttacag tttcaccgcc agaaagcttt
     3241 ctggaaggtt taaaaggaag ttcattgatc aggatatgat gttcatcaat aaggcgcttt
     3301 atctgcactc tagtaatcga aggaagtaaa tcagaaatag tggtatcggc tcttttccct
     3361 gcaaaactct ttggaattat tatttcgtca tatgataatt gtttacccat taaaaaaact
     3421 atgattgcag cttaagtttt tgagattcct ctttttgcat caagattctt ctggcagcag
     3481 tactcacaac tccgggaaca tttttacttc tgcttaacag ttttagatct ttatctctca
     3541 tatgacgaat gaagttcata gaaacatctg ccggtgactt ggggtttgta acaagtgata
     3601 cctttatatc gtagtgttta gtccagtctc tgttattact tatttctctt agaacttcct
     3661 cgctaactga ggttgactgc gctgcaatat taatttccat atctgtcatt cttggatttt
     3721 taagcgctgc aagtgcaact accctgtttg gttctttaac aagtattctt cttgcttctg
     3781 cattacccat gatacataac tttagcttct ctggaagtgt catgcttttt attgcaaaaa
     3841 ataggttctg atattttggg gattgctctt cttcaagatc caattcgcct tcttcaccct
     3901 cttcttctaa aaattcttcc gggatttcta cttcatccag aaaagaactt tcaactgact
     3961 ccacaatggc ttcactttga tcaacttgct gaggctcttc agaactatcc tgtagagctt
     4021 gaggaatgtt aacctctttt tcaagatata gggatataaa cgaaatgacc ttatccagat
     4081 ttgatctgct gatagcaggg tttttactaa gggcatagac tatttcatga gagcggagga
     4141 ttctctgatg gttatcagaa atgagttcga taaggtattg attatgaatg ttttctgcaa
     4201 gatactgtat tgtagaatca gatgttgcat tatttatgat aattttttca aaatgaatat
     4261 cattttctgt atttttagca aaatagtcta ataatccatc aggtacagat tcattggaca
     4321 gtactctttc caaagtatta tacggaagag taaaaagaga ttgtttagat tcatttacta
     4381 cctcatcatc cttgtcaaac gtaagatagt atagcaccaa agccatatcc tcgggaggaa
     4441 tcggtacaag tcccctagcg gccatgattc tagtatcacg gtcgagtgaa ggatctataa
     4501 atctccttag gttttcaggt atggattcta aattgtattc cattttatta gaccgaaata
     4561 ttatattcta tatgaaaaat atatataatt aaacaatatt ttataataaa aatttctatt
     4621 tataattaat taaaaaagat gttattaaaa atgaataaac cgtaaccgga gcaaatatat
     4681 ctctccctta aaatattgga tttatatttt ccaaatacaa tcagtatttg agaaaaagta
     4741 tcgatcttcc tttattaaaa taaaattata gtgttccatt ttccgtctac acaattagca
     4801 tttgagggtt aatacttttt taattttata aaaaaataat aggagcctgg tagattagat
     4861 agcttatgtg acgggatata actaccatat cggcaatggg atttattgat agcatcaatc
     4921 tcatatcatc attcctgcat aaccatcata cataccaaaa tcagtagtca atgttataat
     4981 tggtctgaaa gcagatatat aaattatttt aagattcata aaatcaaatt caccatatta
     5041 tgaaaatcac aattcttggc tctggcactt gtatacccag cataaacaga ggatcatcag
     5101 gttatttttt tgataccggc aaaaataaaa tcttatttga ctgtggaaat ggtattacct
     5161 ggaaacttga acgtctggga gaaagctata cagacataac tcatatattt ataagccata
     5221 tacatcccga tcatactgca gatctgattc cgtttctttt tgccaataaa tatccgtata
     5281 atagaaaaag aacaagaaag ttagaaatct ggggcccgcc cggattcatg gatttctaca
     5341 ctgctattaa caagatatat aatgactggc taaaaccgga gtttttagaa gtttatgaaa
     5401 tggaacaggc tatatatgaa tttaatgact tcacaataat aaataaacct gggaatcatt
     5461 ccacagacag tttgatttac aggattgagt gtggtggaaa gtcagttgtt tattctgcag
     5521 ataccgatta ttgcaagaat ttagctgttt tatctaaaca aaccgacctg ctgattgcag
     5581 aatgttcaat gaaggatgac aagaaagtaa agggtcatat gagtccaaaa gatattatca
     5641 agattgccaa tgaggcaaat cccaaaaata ttgttttaac ccacctgtac ccaatatgtg
     5701 atgaagttga tatcattgga caaataaagc cccatattga atctgatata attaaagctc
     5761 atgacatgat ggaacttaat ctttaactct gaacgcaata tttacaagga ttaattagcg
     5821 taaagcagct ttttggaaga attcacaacc tcatccacgg atatatcttg taaacattta
     5881 acacctatag gacaacttgg tgtatagcca aaacttgtac agggactgca tggcaggttt
     5941 ttgttaatgt taatattatc atctcctttg ggtccccatt tcttttcaat acctgctcca
     6001 aatagtgaga ctgtgggggt acctacacca taagcgatat gcataagacc tgtatctgcc
     6061 gaaacaaaaa gatttagctt ggatattaca actgcggttt ggtatagaga cgttttattc
     6121 gtaaggttta gtatattatc cttgcccact gaccgctcta tccttatgca gtcacccagt
     6181 tcagatttgg ctcccagaat tactacccca atattgcttt ctaaaagttt tgctgtagtt
     6241 ttaatgaatt tatcaactcc ccatatcctc tcagaaaccg tagcgccaca gaatatccct
     6301 gcaacccagt cccatttact gctgtactca ataagttgct tgtttagatc agaacaccta
     6361 ctatcatcaa cggggacaaa tggactgttc ttatcaaatt catactctct tttgctaata
     6421 cctgatacaa gatttaaaaa actttgggat tcgtaatcat catgactata ctggactaca
     6481 tttgtaaaca tcctgcgtct ttcattcgta ccgtacccca cccttacagg cgctcttgtg
     6541 agacaggcaa ctacagatgt taacctgtgc cattgttcag tatctataac aatctcataa
     6601 ttgttactaa atactttttt taaagccttt atggggtcat tatcataaag aatcaattca
     6661 tttatgaatt cacaattctc aaaaataccc tgattccttt tttcggccag tatatctata
     6721 ttacagtttg cgtatatatc ttttagaatc ttaagggccg gaaataacaa aacggcatct
     6781 ccaataccac ccgggcggat tacaaggatt ttcaatttat cgaaattttt agataccgga
     6841 ctttgaagat catcaagaga ataaaactca ctttttctgg gtcttatcaa cctcgataaa
     6901 tgtactgctg gcgtgcctat tatagcatca attttcttta gaatctgaat tttgtatttc
     6961 atccaatatt ctcagggcat catttttttg tgcaggaggt ataccaagct caagcgctct
     7021 tgaggcatgg attcgggcat ttttcaagtc ccctgtttct ttataagcta tagaaagata
     7081 atgatgagaa attccgctat tcggccttat gctaatagaa ttattgaaat actgctttgc
     7141 atattcaaat tcagatatag atttagtctc taaagcagtg agcaggtagt ctatacccag
     7201 attcaaatgg ggagaatgat aatactcatt taactccata gcttttttaa atgtctctct
     7261 tcctttgctc tgttgatcaa tctttatata tgctgtaccc agattattat aaaccaaagc
     7321 cctcacagca tctctgcatt ttatattctt attgttcact acatattcat attgccttat
     7381 agcctcatca attctgccat ttctctttaa tgcgacacca tagttattat aaggcaagca
     7441 agtattactc gacgctacag tcgtagtact ccaaagagag aggtcatcat accatacaga
     7501 ctgtctattg atatttaaaa gcgcataaaa taaaaccagt atagttatga agacacaaga
     7561 atatctaagt gaaactctgc tgaaaacata cttataaaac agatatcctg ttcccattgc
     7621 aaaagctaca gagggtaaat acagatatct ttcagcaagg gatgttggag tcatctttat
     7681 taatgttagt aaaatggatg gtcccaaaga tataagtatc caaaagatca cataatagat
     7741 aagcttaact ctttttatca aggaatatat aaaagctaag aagaccaagg ttaatacgat
     7801 aaatgatgga acaaggtagt ataggtcttt tgtaacttct gagatgaaag cgttaaactc
     7861 aataggaaac aacaatttgt aaaggtaaga ataatatgag cttaccagta ctacaaacat
     7921 atccaggtat ctaaggatat cagctgacgc agcgacattt acattctttt ctgatatggg
     7981 cgtaatattt acaaagctct tactccgtaa atacatatat aaaataaaaa ttgataaata
     8041 taatatatat ttaactgcat tgttcttatt ttttagctga tttgttacca gatcggtcaa
     8101 gagaacaagg ggtataaaaa caacggcaag ttctttagat aacaaagata agtaaaacgc
     8161 cagaatcgca aaaggtatat atactacttt tttgtatgat tttaagtgaa acaatagacc
     8221 cattaataaa aaaagggtgc aaagcacatc agtcctgcca gcaatccaag agacagattc
     8281 tacatgcata gggtgcagcg caaacacaag tgaagatata aaagcaatat tataagaact
     8341 atagtttggt ttaaagttgc tgaagaaaaa atatacaaac cagaaaagca aaattactgt
     8401 aacggtattg aatataaggt tgctaagatg atacccaaaa ggtttattac cccacagagt
     8461 ctgatcaata aaataagata gatatactgt aggtctgtaa taattgcctt tttttgattt
     8521 ttgctgaggt gttagaagat ttataaaacc ctgatactta taatgctttt tattgatggc
     8581 atcagaatcg tcccatacaa gtttattatc aagagacgga agatacaaaa caaaagaaat
     8641 tacgcatagt accagactat aataaatccc tcttttgtat ttagtcatta tagaaataaa
     8701 aataacacat tagaaatctt taaatatata tcattcaaca atgcctgttg caggatcata
     8761 aacgaactcc ctgccatagg gattttc
//
LOCUS       DBNGSV010000345        31260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1448222, whole genome shotgun sequence.
ACCESSION   DBNGSV010000345 DBNGSV010000000
VERSION     DBNGSV010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 31260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31260
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1448222"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1336)
                     /locus_tag="AC159U_12140"
     CDS             complement(38..1336)
                     /locus_tag="AC159U_12140"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3337845.1"
                     /translation="MPNIVVIGTQWGDEGKGRIVDILSEQVNIVARYQGGNNAGHTII
                     VDGKTIVLHHIPSGILRKGKISIIGNGVVIDPKILLEEIDGLQKSGYEVSSENFKISD
                     RTHVIMPYHKLMDKASETQMGDNKIGTTGRGIGPVYEDKVARRGIKVSDLLYPEYFEQ
                     RLKSIIEQKNIYIEKVLEAKPLDFNEVFNEYIAYGQQLKPYITDTSLMLHDNIKRNKT
                     VLFEGAQGVMLDVDFGTYPYVTSSNAGAGGVCAGTGISPKSIDHIVGISKAYTTRVGE
                     GPFPTEITDDFGEVLRKAGNEYGATTGRPRRCGWFDAVAVSYSVRVNGINSIMLTKLD
                     VLSDLESIKICTGYKYKDQILTDFPSNLNVLTECIPVYEQLPGWKSDLSCISDPKDLP
                     QNLISYLNRIEDLLETEISLVSIGPSREEFIALKNGLIAN"
     gene            complement(1338..2585)
                     /gene="hisZ"
                     /locus_tag="AC159U_12145"
     CDS             complement(1338..2585)
                     /gene="hisZ"
                     /locus_tag="AC159U_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3337846.1"
                     /translation="MSKNLSLPQGVRDFTPERAEEIRKVEEFLLEEFARWGYRRIITP
                     LFEYLETISSGLGEDFEKKLMKFVDPSTGEVVALRPDITPQVGRLIASRLRDEIKPLR
                     LCYNERVIRFEEKGSGKEREVFQVGCELAGMQSAETDAEIIALSIKCLGKVGIKNLVL
                     DIGHNGLLESLLLQTGDARSYIEDALKKKDQEALEQAIKNKRINKSVKEMILMLPQMY
                     GGSEVLAKAKKYKLLKNYVQELENVLEVVEEYNLNCRVNVDLGELRGFNYYSGVTFEI
                     LSPNNFNPLIRGGRYDNLIKKYGYDIPSTGFAVDVEAVLSITKTHLENTPVHFVVIPK
                     KENLRRESIRLAEWLRSSGFKVVLDLKSDANLKKRRIQENSKSNSYGIILLETPHKIK
                     LIESRSGVSREFSDLEELLKGGI"
     gene            complement(2586..4175)
                     /gene="serA"
                     /locus_tag="AC159U_12150"
     CDS             complement(2586..4175)
                     /gene="serA"
                     /locus_tag="AC159U_12150"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3337847.1"
                     /translation="MKVLISDGLSQDGLDILESSGKIQVDVKKGIEKDELLKIIKDYD
                     ALIVRSATKATKEVIDAAEGKLRVIGRAGIGVDNIDIPYATKMGVVVMNTPESNAITT
                     AEHAIAIMFALARKIPQAHKSLTSGEWKRDKFKGTEIYGKTFGVIGLGNIGKLVAERA
                     LGLQMRVIAYDPFITEEAASKLGVELVELDDLIKTAEIITIHTPLTPETKDLINKDTF
                     DKMKDGVLLINCARGGIVNEQDLAAAIKEGKIGGAALDVYVNEPPAADHELFSIEDNV
                     VFTPHLGASTSEAQAKVGIAMAEQIQDFLLNDVVRNAVNMPSLSLDILKRMKPYLTLL
                     EKLGSLQGQLCKGGIEEISIEYRGDVSEFDVSPLTVAALKGFLTPIMDLVVSFVNAPV
                     IAKEKGIKIVESKFSDSEDYTSLVTIQVKTDEGKTRVSGTIFGREEPRIVGVNGFPID
                     VIPEGYLLINENADKPGFIGALCTLLGSKDVNIARLHLGRESIGGKAISFINIDSPVS
                     KEVEQEISKLPDHISVTQVKL"
     gene            4332..5072
                     /gene="tatC"
                     /locus_tag="AC159U_12155"
     CDS             4332..5072
                     /gene="tatC"
                     /locus_tag="AC159U_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3337848.1"
                     /translation="MSEEAKMPFLKHIEELRKRLIYAVIAILICFIPSYYFSDLIFDF
                     LMQPLIASLPEGSTMIFTRPAEGFTTYLKVAFFAAVFISVPALLYQAWQFVAPALYKN
                     EKKILLPFLFFGTFFFISGGAFCYYVASPPAFNFLLNEYSSEYVKAFPSIREALAFFM
                     ALILGFGFIFEFPLVSFILAKIGIVTSKMLSKNRRYALLISCVVAAILTPTTDALSMM
                     FMFIPLIVFYELGILVAWIFGKKAPPED"
     gene            5151..6860
                     /locus_tag="AC159U_12160"
     CDS             5151..6860
                     /locus_tag="AC159U_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3337849.1"
                     /translation="MPNAKKTYDLIHPITYKRKTEPFDVEWYKNEDKNNWVLPKVLKK
                     QAKELGKKPFLQFGYKKPISFFQTNQLANKVANGLSKLGIEKGDKVAVYMPNSDDYVM
                     TWFGILKMGAVMVPINTAYKMDFLQYIIDSSDSTVLFIAEEYLDRMKPIAKKIPQLKH
                     VIVWTRDGGEKFDKHGFNFRKMTSYAKFMKSAKTAEPKVEITFMDHARLMYTSGTTGR
                     SKGVVRPCAADYSSAYNYAQIMDVGPDDVCFTCLPLFHSNAMVMSVYPALIKGCKSVV
                     EEKYSASKFWNWIKDHEVTKFNIVGTMSYFMWNSPVVPEEKQHKAELVLGSPAPHDII
                     KEFMKRFNIKFMEGYGLTEIGQCTWMRPGEPFRVGSCGKEAPGYEIKIADPDTDEELP
                     RGQVGEIIVRPRTPNVMLHYYNKMYEKTVSDFRNFWFHTGDAGRMDKDDYIYFVDRVK
                     DYIRRRGENISSFELEKIVSSYPDIKESAAVAVKEEKGKYAEDEVMIVIVPEKGKKID
                     FIKLMKFLEPRMPYFMIPRFVRIDKSLPKTGTQRVQKNKLREKGITKDTWDREKAGYK
                     IKR"
     gene            complement(6866..7219)
                     /locus_tag="AC159U_12165"
     CDS             complement(6866..7219)
                     /locus_tag="AC159U_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3337850.1"
                     /translation="MSSETQKIIDHHLEAFEQGDVDGLMLDYTESSTLITPERILTNL
                     NEITALFNKFLADVVPPGCDFELYKKIINDDIAYLIWSAESENYKIPFGTDTFVIKDG
                     KIATQTVALVLESKN"
     gene            complement(7245..8252)
                     /locus_tag="AC159U_12170"
     CDS             complement(7245..8252)
                     /locus_tag="AC159U_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337851.1"
                     /translation="MSTLTIDNKLWIKNPLTDSIYFSFGWILVFLALLIFQNHLGLII
                     VITLLFNYVHRHLTMALVYGQKEEFDKRRQVYIYLPVIAVVATALSVYFKVFPYLLTL
                     SVLWTMYHSVAQKYGFTRIYSRKAGYGKGWVDKGILYSWFFYLFFAMLLYEQSTILKY
                     NAGRVILGHAAPYMGYIASLSYILIAVSIIFTLIYIYQEFINRKQISIPKNIFVISTL
                     SIYAIFFHSMIIGYVVFGFSHAIEYIAFVNIFVASKYKKVDDSKKLFKKISNKQWLYS
                     GAFSAVIVVLSLIGMKLDQNAFLIYIVGSSFLHFIYDGLIWKVRQPEVGRPLDIKYSS
                     A"
     gene            8459..9547
                     /locus_tag="AC159U_12175"
     CDS             8459..9547
                     /locus_tag="AC159U_12175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3337852.1"
                     /translation="MKLGFFTMPLHPPGRELHETLNDDLKQLIFLDKLGFSEAWIGEH
                     FTAGWENIAAPDLLIAKALSLTKNIILGTGVSCLPEHDPFVLAHRIAVLDHIAKGRFY
                     WGIGAGSFIGDFDAFGIDVKSGEQRTLTNESLDFILNLWNDPKPGDYQNSRWKFRVPE
                     PQQDVALGVHTKPFQKPHPPIGVAGITEKSGTLQIAGEKGWIPMSINFAPVATLKTHW
                     DSVQQGAENVGKTADRSMWRIARDVYIAETTEQAREEVKNGTLARDFTDYFFKMVPKI
                     RGNLNIFKTDKSMPDSDVTIDYMIDNLWIVGSPEDAVKQIARLHDEVGGFGTLLVMGH
                     EWKPEDKWQNSMRLLKEEVIPGLKKLGI"
     gene            9603..11693
                     /locus_tag="AC159U_12180"
     CDS             9603..11693
                     /locus_tag="AC159U_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR3337853.1"
                     /translation="MGHSKGRLFLRALKNKSNQVQQKLKSLGVPDYTIFVILSIVTGA
                     ASGSVAVFFHKFIDLIDYFLFDFIRSSVPALSSFTVILFPAIGMLCVYLLIRLAPKVS
                     KRKGVMEVIKAVATRGGHIPFRTTLFNFIAPSICIGSGCTLGPEGPAAQIGGGIASKL
                     GRIFGLSDTRRRMFTAAGAGAAIAAVFRTPMGGVFFALEVVLLNDFQAPIFSALILAS
                     VTASAISQMMLGDKPIFFFDNINTGPYSEFYLYAVCGILAGGLSVLFIKYAEYIKEVF
                     KQKNIQEIPKWKIMGGVGLLVGICGLFFPEILGVGYKAINGFLSDTITWEIALVLFVL
                     KFVLVPIVLYAGGFGGVFAPSLFMGASFGFVFSIFLNQAFGFNLDTTAFTLVSMGAFL
                     GAVNSIPISAILIIFEMTQDYSFILPLMLSVVSSTTIAQLYFKGSIYSKHLEEEGYHI
                     TQGHDTDILKSLRVSDVMRDDIVLIPENLTLPTLISKLGETPHSTFYTINPEGKLLGT
                     IYESELRPVLTEYENLREMIVAGDIAKPGIIVVRENDDLDFVLKLFGQENVDEFPVVS
                     SEDSSKVLGSVNYHDILAAYNRETLKHHLADGLVHDLKAIEKFTSVKVSEGYSISEQL
                     APKQFVGKTLAQLRLRNVYGVEVLMIRPHHHHFIEEEKKTEFIVPRHDYTIETGDILI
                     VFGNDESIGKLKEL"
     gene            complement(11724..13367)
                     /locus_tag="AC159U_12185"
     CDS             complement(11724..13367)
                     /locus_tag="AC159U_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR3337854.1"
                     /translation="MKLQEQISKRRTFGIISHPDAGKTTLTEKLLLFGGAIQTAGAVK
                     SNKIKKSATSDFMEIEKQRGISVATSVMGFEYDGYKINILDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVKGVEEQTRKLMEVCRMRHTPVIIFINKLDREGQDPFELLDEIEKELD
                     IHVRPLSWPIGMGKNFKGVFNIHENKLQLFESEKTKQSDSHVEITDIDSTKLDEQIGD
                     SNAAKLREDIELISGVYEDYDEGLYRDGYLAPVFFGSAINNFGVKELLDTFVRIANEP
                     QTRMADKRLVEPSENKFTGFIFKIHANLDPNHRDRVAFCRIVSGTLERNVYYHHTRIN
                     KKLRFSNPTSFMANDKNIVEQAFPGDVVGLYDTGNFKIGDTLTEGEVLQYKGIPSFSP
                     EIFRQLINRDPFKSKQLDKGIRQLTDEGVAQLFVREEGNVKIIGTVGELQFEVIKFRL
                     LHEYGAACEFQHLNFYKACWITSEDKKALEEFARRYSRLVTKDKDDRLVYMAENAWML
                     EDKQKTFPEIEFHFTSEFKTESSKPKTTDTDLTINRDVA"
     gene            13552..13740
                     /locus_tag="AC159U_12190"
     CDS             13552..13740
                     /locus_tag="AC159U_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337855.1"
                     /translation="MSTAKDEIKALLENLPEDCTFEDVQYHLYVVEKIQNGILRADQE
                     GTISQSEVERKFEKWTSK"
     gene            13725..14036
                     /locus_tag="AC159U_12195"
     CDS             13725..14036
                     /locus_tag="AC159U_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3337856.1"
                     /translation="MDKQVIWSPEAVDDLHSIVEFISKDSEYYSSAVTTKILTTTKQI
                     GNFPLSGRIVPELNNEKIRERLVYSYRVTYIIREESVLITAIIHGKRLIENIEDRIKD
                     T"
     gene            14155..14940
                     /locus_tag="AC159U_12200"
     CDS             14155..14940
                     /locus_tag="AC159U_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HIR3337857.1"
                     /translation="MNEITVSSWNELQTELFTESWNEKLGRFRSRYAFRGLSDREYKL
                     ESRLYRHGGDFKKLEKHLLRNFKKYAHRDVVEKDSIWNWLSVAQHYGLPTRLMDWTYS
                     PFIAMHFATANLDKFHTDGVIWALNYVHTHNLLPNKLRKKLDEEGANVFTVEMLFESI
                     KSISELEQLSKKPFVMFFEPPSIDDRIVNQFAFFSVSSHSNIVLNTWLEEKQDIWRRI
                     IIPARLKWEIRDKLDQANITERVLFPGLDGLSKWLARHYSPKV"
     gene            15069..15815
                     /locus_tag="AC159U_12205"
     CDS             15069..15815
                     /locus_tag="AC159U_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3337858.1"
                     /translation="MELNEKQLKMCSESKWDFSDLKALFLNCTLKKSPEVSHTQGLID
                     ICKAIMEKNKISVEVLRPIDFNLAFGVYPDMTEKGWDVDDWPQIYEKVKAAHILVITS
                     PIWLGEKSSVCTQTIERLYSSSGDLNESGQYAYYGRVGGCLITGNEDGAKHCSMNILY
                     SLQHLGYVIPPQADAGWLGEAGPGPSYLDEGSGGPENDFTNRNTTFMTWNLIHMARMI
                     KDAGGIPAHGNQRSEWDAGCRFDYPNPDYR"
     gene            15878..16255
                     /gene="crcB"
                     /locus_tag="AC159U_12210"
     CDS             15878..16255
                     /gene="crcB"
                     /locus_tag="AC159U_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR3337859.1"
                     /translation="MVNILLVGLGGFIGSVSRYMLGDLVHRLLDKPVFPYGTFVVNIF
                     GCLLIGILGGLSESKNLFDTQTRMFLFIGLLGGFTTFSSFGFETFNLAKQGAHFHALT
                     NVFLQIVLGLIAVSIGYIAAKNI"
     gene            16328..17119
                     /locus_tag="AC159U_12215"
     CDS             16328..17119
                     /locus_tag="AC159U_12215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3337860.1"
                     /translation="MYRNMQIALIISVMLFSISCAGKNMFTEGQWIDLTHEFSEQTIY
                     WPTSSTFKKEPVYEGHTEKGYYYSAYKFSTAEHGGTHIDSPIHFYEDRNTVDQIPIEQ
                     LIGPGVVVDVSKYALTNPDYQVMVTDILKWESANGQIPDGAILLLNTGYGKFWPDRVK
                     YMGTDQLGQDAVKDLHFPGLHPDTAKWLVKNRKINAVGLDTPSIDYGQSTHYGSHVIL
                     FEANIPAFENVANLDKLPATGATVFALPMKIKGGSGSPLRIIANF"
     gene            17181..17423
                     /locus_tag="AC159U_12220"
     CDS             17181..17423
                     /locus_tag="AC159U_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337861.1"
                     /translation="MSAEAMEHIGESQGMENHDHDMIHDLSLRLDALWRYDQYIANAK
                     GNRSLIAFWKKIKDQESSNIQSLKKHIAAHAKKGCF"
     gene            17528..17884
                     /locus_tag="AC159U_12225"
     CDS             17528..17884
                     /locus_tag="AC159U_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337862.1"
                     /translation="MNRKSLLLLSSLFFCIFTLSGCEMISGFLNPFSGSWKSGLFHFT
                     FNTDNSFKLEVGSGLSFKTEGTYKYDKEQITLSFSKDSNTTFTYVFNENKSELTLTPK
                     TEFKWFKTAVKFTKSN"
     gene            17955..18641
                     /locus_tag="AC159U_12230"
     CDS             17955..18641
                     /locus_tag="AC159U_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR3337863.1"
                     /translation="MSNHIHDKSFPGESKEYRSARNELLKAEIELRQRIEEVGRMKQA
                     LPLGGKLKKDYVFKELINNEEREIKLSELFKDNKKSLIIYSYMYGPKMENPCPACTSL
                     IDGFNGSAMHITDRVNLAVIAKSPIKRIVEFADSRNWKNIHLLSSENTSYNADYFAET
                     SEGNQIPACNVFTKTPDGIFHYYAAELLYAPLDGHPRHMDLMWPVWNLLDITPEGRGT
                     DWHPKLSYDK"
     gene            18907..19047
                     /locus_tag="AC159U_12235"
     CDS             18907..19047
                     /locus_tag="AC159U_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337864.1"
                     /translation="MADSNGKAFLKYIGKRKWRRVKSTKNSPEKNFLTYLGNRKWKKA
                     KS"
     gene            19304..20608
                     /locus_tag="AC159U_12240"
     CDS             19304..20608
                     /locus_tag="AC159U_12240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3337865.1"
                     /translation="MKNDLPDKQQNENLKDKDSRISELEKILEISKELTSSLRLDPLL
                     KKIVETASELTDSEGATIFLLDPKDGNLYFRATTAQSSNLLSEVPVPLDDSIAGQVHN
                     TKKPLIVDDVQSDPHFYKGIDQILGFKSNSLIGVPLIIKERSIGVLEGVNKKGQKEFT
                     QKDVDTLVVLASHAAIAIENARLYESVVNQAEILEQQVEERTATLKARNKELTAYDQT
                     VAHDLKNILTHIIIYAENLNEFSDVVNDEEFDSNLDNISNMSFKMNNIIEELLLLSGV
                     RNTEVQTNPVDMSKIITEAQKRLSLMIEEKNAEIKASQSLPSAMGYAPWIEEVWFNYL
                     SNALKYGGDPPVIEIGSKEEPDGNISFLIKDNGQGIPVDKQDDIFVRFTSSKKDFSMG
                     HGLGLSIVKRIVDKLGGEVGVQSEQGTGSTFFFKLPKAQSLN"
     gene            complement(20605..22059)
                     /locus_tag="AC159U_12245"
     CDS             complement(20605..22059)
                     /locus_tag="AC159U_12245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3337866.1"
                     /translation="MNKIKVKKTILITGASGYIGGRLLKTLQESNEYDIKCLARKPEY
                     LKSKVPNTVKIIKGDVLDIESLEKALVGIDTAFYLIHSMGSKDGFEDKDRQGAENFVK
                     AAENAGIRRIIYLGGLGDSSKVLSPHLKSRHETGQVLRSGKVQVIEFRASVVIGSGSL
                     SFEIIRALVEKLPVMTTPKWVYVDTQPIAISDLLNYLTKAITLKVKGNQIFEIGCKDV
                     VTYGELMQEYGRQRGLKRYIIPVPVLTPWLSSLWLGLVTPVYARIGRKIIDSLKNPTV
                     INDHLAKDHFDIETLTVSDAIKEALAEEEKQFYENRWSDSLSSGDTLRNWGGIKFGTR
                     IIDTRRKEVNASVKRAFLPIRRIGGQTGWYYADWLWRFRGFIDLLVGGVGVRRGRRDP
                     EDIAVGDVVDWWRVEDYEPNKLLRLFAEMKLPGRAWLQLEAKKIEGKTYIIQTAIFDP
                     MGLGGLLYWYALYPLHAIIFTGMLNKISKEASAS"
     gene            22226..22660
                     /locus_tag="AC159U_12250"
     CDS             22226..22660
                     /locus_tag="AC159U_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3337867.1"
                     /translation="MNTVSDMLQEKGTGLCTISPNATVYEGLQIMAEKDVGAVLVTQN
                     NKLLGIFTERDYARKVILQGKSSLNTAIKELMVKDVLIVKPSDSIDQCMAVMTERQIR
                     HLPVMQGDELLGLVSMRDVVNHIISHKNFKIKELEKYITGSY"
     gene            complement(22664..23491)
                     /locus_tag="AC159U_12255"
     CDS             complement(22664..23491)
                     /locus_tag="AC159U_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3337868.1"
                     /translation="MSQSKILNINNISITFNPDTLNEVKALWGVDLSIEQGSWVVVIG
                     TNGSGKSTLLNAVAGNVMTDSGSIELSGNDITRWPEHKRANLIGRVFQNPFSGTAPNM
                     TIAENLALASKRGQSRGLDMALKSEVFESFRQRVSTLNMGLEGRLDNIIGRLSGGQRQ
                     ALTLLMATWQRPELLLLDEHTAALDPKTANQVIKLTEELVSKENLTTLMVTHSMQQAV
                     ALGDRLIMMHGGRVIHDFRGAEKKRLRPKELYERFEDVRRREQLDETAAEMLKRLYI"
     gene            complement(23469..24407)
                     /locus_tag="AC159U_12260"
     CDS             complement(23469..24407)
                     /locus_tag="AC159U_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3337869.1"
                     /translation="MSLLIGALIIGLILSLLALGVYISFRIFDFPDITTDGSITTGAA
                     VTALLLVNGYSPLLATGFGFICGMLAGTVTGVLYTKFKINNLLSGILVMTSLYSVNLH
                     IMGKSNIPLINTKSLVTTVENIWHKLFGESAVVDVLGWDVSTSDLSVLFAMLILIAVV
                     GCVVYLFFKTNIGTAMRATGDNPQMITALGVNVENMTILGIALANGLIALAGSLLAQF
                     QGFADVQMGIGMIIWGLASIIIGQALVGTPSLGFTIAGAVIGSVLFRLLVAIALRVGV
                     NPNDLKLITAIFVFIALVLPSLISKFNKKNVTVKNT"
     gene            complement(24413..25651)
                     /locus_tag="AC159U_12265"
     CDS             complement(24413..25651)
                     /locus_tag="AC159U_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3337870.1"
                     /translation="MEIKRQHQEGFEILILDGRLDAYWADHLESELSKSIREGHYKIR
                     LDMAGVNYLSSAGIRILLQSYKKLNQINGQFSLVNISQNVQSVLELSGVAALLKPHKT
                     EDTTSITKGAETKRINTENGVFEILHEDENSSLSCEIKGSIEFIDHNNKKIINIPIQI
                     SGDCYALGIGAFGNGYDDCKNRYGEFAALAGAVSYLPTDGTNVADFHIASGDYLPEVN
                     ALYSITCKGEFGSMTRFDPAKEKEVIGFSELIEAGFNISGKDTIAIAAVAESAGIVGA
                     NLIKSPVADESGIDDIFSFPNIQNCISFTSEKSYIGSLVVIFGVASKEQSGDLGEFLR
                     PLGSTGLFGHFHAMIFSYEPFMKGKLDLKETIKNLFDRQSLQSVIHLLSDERDIVGAG
                     ESEFVRGGLWVSPIESVIKA"
     gene            complement(25651..27624)
                     /locus_tag="AC159U_12270"
     CDS             complement(25651..27624)
                     /locus_tag="AC159U_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3337871.1"
                     /translation="MKRLSLSIFLIVLASSVLLLSDLNQRKRTKSEIPKIAMLQLSSH
                     IILEDGIRGILDGLKEKGFIDGQNIDIQFYNAEGDIPTLNTIASEITSGKFDYALTVS
                     TPALQAVSNANKQGKVVHVFGLVTDPFASGIGLDRAKPEDHPEYIVGYGTQQPVEKLI
                     ETAMKLNPNIKSIGMLWNSAEANSVVCTIQARNYSKLVGVELLEATVENTSVVYETAA
                     SLVSRGVDALLVSGDNTVITALDNVVQAAKNGKIPVITNAPTFPNKGTLLDIGADYYE
                     VGKRQGYLAARVLNGEDPESIPIRNLAPEVVVINKQAIESLKDNWRIPEDILKSVDVV
                     IDENGKEIRKVNTVENDSEEKIRLTKRWNINVVEFIEVSDVEETRKGILEGLQHAGLK
                     EGRDYNIKIQNAQGDMPTVSLMIDNALSQGADMLMTLSTPTLQAAIQRAKNIPVVFTY
                     CASGVNAGAGRTNEDHLPNITGVYALPAYGEMMALFKKLMPHAKKIGTLFVPAESNSV
                     LHNKLLGVEAKKYGVELVSLPANTSTEVPDAALAMAASDIDAIIQIPGNLTAVAFTSI
                     AKAANQSKLPIFAFQTVQSAEGASIVLAKDYYDFGKEAAKLAVRVMRGENPKDMPFVG
                     LEETSLILNLDAAKISNLHIPESLNAGAKTVLR"
     gene            complement(27702..28739)
                     /locus_tag="AC159U_12275"
     CDS             complement(27702..28739)
                     /locus_tag="AC159U_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3337872.1"
                     /translation="MKRLYFSIFLILLASLVLLLSDLNQRKKNERTIPKVAILQFSSH
                     TFLDDGVEGIFDGLKESGFEDGINIHIKHFNSEGDLPTANAIASEITSGKYDYALTVS
                     TPALQAVANANKQGKVIQIFGVVTDPFIAGVGLKKNDPLDHPKHLVGYGSFLPVIKLI
                     KIAKELYPDLRSIGLAWNAAEANSEAYTILAREFTKQENLKLVEVIVDSSVAVNEAVN
                     SLVSRGVDMILVTGDNTVGLALDNVVDAASKGKIPVLTLIPTFPNKGTLLDLGADFYQ
                     VGKLQGYLLSSIINGKDPATIPIENVVPEVLIINKQVLRNLKDPWKFTDKLIQKADVI
                     IDEKGKEIRTK"
     gene            28998..29594
                     /locus_tag="AC159U_12280"
     CDS             28998..29594
                     /locus_tag="AC159U_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR3337873.1"
                     /translation="MSYSVEGQLLEVCNCNILCPCWVGGDPDEGYCEAIVGWHIKEGE
                     IEGVDVSGRTLAMLTHIPGNVLDGNWKVRLYVDQDTTDAQQEGILNVWGGKLGGAITD
                     LAGLVGEVVSVEKTNITFDVEGVNGTIKVGDVAQASLEAFKGGTGLETTLHDSIFSTI
                     PGKPAYVGKASSFKANAPGFDIDLQDHNAICGHFKFEA"
     gene            29637..30443
                     /locus_tag="AC159U_12285"
     CDS             29637..30443
                     /locus_tag="AC159U_12285"
                     /inference="COORDINATES: protein motif:HMM:NF021465.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR3337874.1"
                     /translation="MSIFYKASFSPRVERGILITVLSVLALLCWIVLWKGGDLAHVAH
                     NFGTQLHNHHSGHHSANSYSFMVIFIASWSVMTVAMMLPTTIPIVAFFHKFTENRADR
                     VLLLSLLLLGYVGTWTVFGVLIYFQIALIKWALASFSFLSDYMWTGPPLILIFAGLFQ
                     FTEIKYRCLDKCRSPLSFITERWRDTKEKWNAFMLGVDHGKFCIGCCWALMLLMFAVG
                     FGSLIWMLVLALVMGIEKNVKWGRKIGKPVGYILVVSGLFMLVYTYAKIY"
     gene            complement(30454..31173)
                     /locus_tag="AC159U_12290"
     CDS             complement(30454..31173)
                     /locus_tag="AC159U_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337875.1"
                     /translation="MNKISFLSVYAVLLSVVILTSGCAYGLRANRTYIKIDPQNISTP
                     KLANKVNFSISANVWKGEPKRLRRHLIPFFIEVQNNTDKNIIISEADFILLDENRNQF
                     NALSPERAANIVKSAEQKRFRIYPRISIGIGTSFHHDRFHYYGHHHHPFYRPYYYYDD
                     YPYPYYRRQDYEPDLEDIYAKALSTGVIRPGATLSGYIFFNKISRDTKELTLEIGYTE
                     KENNTAHKLDFHFDLIEVYYK"
BASE COUNT         9525 a         6157 c         5726 g         9852 t
ORIGIN      
        1 atgccaagca gatactgccg ccgtctactt tccattatta atttgcgatt agtccgtttt
       61 taagagcaat aaattcttct cttgatggac cgatagagac taaagagatt tctgtttcca
      121 gcagatcttc aatcctattc aaatatgata ttagattttg aggtaaatcc ttaggatcac
      181 ttatgcaaga taagtcagat ttccaaccgg gcaattgttc ataaaccggt atgcactctg
      241 taagtacatt taagttagat gggaaatctg ttaatatctg atctttatat ttatatccag
      301 tacagatttt gatgctctcc aggtctgaaa gtacatctag ttttgtaagc ataatactat
      361 ttataccatt taccctgaca gaataggaaa cagccacggc atcaaaccag ccgcatcttc
      421 ttggtcttcc tgttgtagca ccatattcat ttcctgcttt tcttaaaacc tcaccgaaat
      481 catcagtaat ttctgtagga aacggcccct caccaaccct tgttgtatat gccttgctta
      541 taccaactat gtgatcgatt gatttcgggc ttatgccggt acctgcacac accccaccag
      601 ctcctgcatt ggatgaagta acatatgggt aagtgccaaa atccacatcg agcattactc
      661 cctgagcgcc ttcaaacaat actgttttgt tacgtttgat gttatcatgc aacattaaag
      721 aagtgtctgt tatataaggc tttaactgct gaccataggc aatgtattca ttaaatactt
      781 cattaaaatc cagcggctta gcttctaaaa ctttttcaat atatatattc ttttgctcaa
      841 ttatgctttt taatctctgc tcaaaatact caggatataa taggtctgaa accttgatac
      901 ctcttcttgc aaccttatcc tcataaaccg gtccgatgcc ccttcccgtt gttccaattt
      961 tgttatcacc catttgcgtt tcactggctt tatccataag tttatgatag ggcattatca
     1021 catgggttcg gtcactgatt ttgaagtttt ctgacgatac ttcatagccg cttttttgca
     1081 ggccatcaat ttcttcaagc aatatttttg ggtcaatcac aaccccgttg ccaattattg
     1141 aaattttgcc ctttcttaat attccagacg gaatgtggtg aagaactatt gtcttgccat
     1201 ctactatgat ggtgtgccct gcgttattgc cgccctggta ccttgcgaca atgttaacct
     1261 gctcggatag aatatctaca atcctgcctt taccctcatc accccactgg gtaccaataa
     1321 ccactatatt gggcaaatca gattccccct tttaatagtt cttccaggtc agagaattct
     1381 ctgctaactc cactgcgaga ttcaataagt tttatcttat gaggggtttc aaggagtata
     1441 attccatatg agttggattt actgttttcc tggatacgtc gttttttaag attagcatct
     1501 gattttaagt ccagaacgac cttaaacccg cttgatctta gccactcggc aagccttatt
     1561 gattcccttc taagattttc ttttttagga attaccacaa aatgaacagg agtattttcc
     1621 agatgtgttt tagttatact taatacggct tccacgtcca ctgcaaaacc ggttgaagga
     1681 atatcataac catacttttt tatgaggtta tcgtaccttc cacctcttat tagaggattg
     1741 aaattatttg gtgatagaat ctcaaaagta actccggagt agtagttaaa ccccctcagc
     1801 tcacccaggt ctacatttac cctgcagtta agattatatt cctctactac ttccagcaca
     1861 ttttctaatt cctgcacata gtttttaagt agtttatatt ttttagcttt ggcaagtact
     1921 tcggaacctc catacatctg cggaagcata agtatcattt cttttactga tttatttatt
     1981 cttttatttt ttatcgcctg ttcaagagct tcctgatctt ttttcttgag tgcatcttct
     2041 atataacttc tagcatcacc agtctgcaag agcagcgatt ctaaaagacc gttatgtcct
     2101 atatctaaaa ccagattttt tatacctact tttccaaggc acttaattga aagtgctatg
     2161 atctcagcat cagtctctgc tgactgcatt ccggcaagtt cacagcctac ctgaaacact
     2221 tctctttctt tgccgcttcc tttttcctca aaccttataa ccctctcgtt ataacaaagc
     2281 ctcaaaggtt tgatttcatc tcttagtctt gaagctatta gccttcctac ctggggggtg
     2341 atatcaggtc tgagcgcaac tacttcccct gtcgaaggat ctacaaactt catcagtttc
     2401 ttttcaaaat cctccccaag tcctgatgaa atggtctcaa gatattcaaa aagaggggta
     2461 ataattcttc tgtatcccca ccttgcaaac tcttcgagca ggaattcttc aacttttctg
     2521 atttcctccg ccctttctgg agtaaaatct cgtactccct gagggaggct taagtttttt
     2581 gacatttata gtttcacctg tgtaactgag atatgatccg gcagcttact tatttcctgc
     2641 tcaacttctt tagaaaccgg actgtctata ttaataaaag atatggcttt gccgccaatg
     2701 ctttctcttc ctagatgaag ccttgcaata tttacatctt tactgcccaa aagagtacac
     2761 aatgcgccaa taaaaccggg tttatcggca ttttcattta taagcaggta accctctggt
     2821 attacatcaa ttgggaaacc gtttacccct acgattcttg gctcttctct gccaaatata
     2881 gtacccgaaa cccttgtttt cccctcatcg gttttaacct gtatggtgac aaggctagtg
     2941 taatcttctg aatctgaaaa ttttgactct acaatcttga ttcccttttc ttttgcaatt
     3001 acaggggcat taacaaaact taccaccaga tccataatag gggttaaaaa tcctttaagc
     3061 gctgctactg ttaacggtga tacatcaaat tcagacacat cgcctctgta ttcaatacta
     3121 atctcttcga tcccgccctt gcagagctgc ccctgaaggc ttccaagttt ttccaaaaga
     3181 gtaaggtaag gcttcattct ttttagaata tccagactga gcgagggcat attcacagca
     3241 tttctaacta catcatttag caggaaatcc tgaatctgct cggccattgc tatgccaact
     3301 tttgcctgag cctcggaggt agacgctccg agatgtggag tgaatactac attgtcttct
     3361 atcgaaaata gttcatgatc tgctgctggg ggttcattta cataaacatc cagagcagcg
     3421 ccgcctatct tgccctcttt aatagcagcc gccaggtctt gttcgttaac tatgcctcct
     3481 ctggcgcagt taataaggag caccccgtct ttcattttat caaatgtatc tttgttgatt
     3541 aaatccttgg tttctggagt tagcggagtg tggattgtta ttatttcagc ggttttaata
     3601 agatcgtcca gctcaacaag ctctactcca agcttcgatg cggcttcttc agttataaat
     3661 gggtcatagg ctatgactct catttgtaga ccaagagccc tttcagcaac aagttttcct
     3721 atattaccaa gacctattac cccgaaggtt ttgccataaa tttctgtacc cttaaattta
     3781 tctcttttcc actcacctga tgtaagggat ttatgagctt gaggtatttt tcttgcaaga
     3841 gcaaacatga tggcgatagc gtgttcagca gttgttatgg catttgactc aggagtattc
     3901 ataaccacaa cacccatctt tgtagcatag ggtatgtcga tgttatcaac accaattcct
     3961 gcccttccta taactcttag ctttccttct gcggcatcaa ttacttcctt ggtagctttt
     4021 gtcgcactcc ttactattaa tgcgtcgtaa tctttaatga tcttaagaag ttcatctttt
     4081 tctataccct tttttacgtc tacctgaatt tttccgcttg actccaatat atcaagcccg
     4141 tcttgtgata gtccatctga tattaatact ttcaattttt ttactccatg aatttaatag
     4201 ggatgaattt aacggcataa caatataagt aactacgtgt taatgtcaat gatgtcaggg
     4261 caagaaatct tatcaaaatt agctcatcaa attgttaatt aattcttatt tgttatgatt
     4321 ctattcccaa tatgagcgaa gaagcaaaaa tgccgttttt aaagcacatc gaagagctgc
     4381 gcaagagact gatctatgca gttatagcaa tcctgatatg ttttattcca agctactatt
     4441 tttctgacct tatatttgat tttttgatgc agccgctaat tgcaagcctt cctgagggaa
     4501 gcacaatgat attcacaaga cctgctgaag ggtttacaac ctatttgaag gttgcttttt
     4561 ttgctgcagt ttttatttca gtaccggctc ttttatacca ggcatggcag tttgtagccc
     4621 cagcgcttta taaaaatgaa aagaagattc ttctgccatt tctatttttt ggaacattct
     4681 tttttatatc cggtggagcc ttttgttact atgtagcttc accaccggca tttaatttct
     4741 tactcaacga atattcatcg gagtatgtaa aggcattccc atccataagg gaggcacttg
     4801 ctttttttat ggctttgata ctggggtttg gctttatatt cgagtttcct cttgtaagtt
     4861 ttattttggc caagatagga atcgtaacct caaaaatgct cagcaaaaac cgccgctatg
     4921 cgcttcttat aagctgtgtg gtagctgcca tactaactcc tactacagac gcgctgtcca
     4981 tgatgtttat gtttattccg cttatagttt tttacgaact tggaatatta gtagcctgga
     5041 tttttggaaa gaaggcacca ccagaggatt aatatttaaa aactaattga aaaatatcat
     5101 taaatattta atatcttaaa attaactaca aacatactga ggaacttaat atgcctaatg
     5161 caaaaaaaac atatgaccta atacacccga tcacttacaa aagaaagact gaaccatttg
     5221 atgttgagtg gtacaagaac gaggataaaa ataactgggt acttcccaaa gtactgaaaa
     5281 agcaggccaa ggaacttggc aaaaaacctt tccttcaatt tggctataaa aaaccaatca
     5341 gtttttttca gacaaaccag ctggcaaaca aagtggcaaa cggactgtct aaacttggta
     5401 tagaaaaagg cgataaggtc gcagtctaca tgccaaactc agatgattat gtaatgacat
     5461 ggtttggaat attaaagatg ggtgcggtaa tggtgcctat aaacacggcc tacaagatgg
     5521 attttcttca gtatataata gacagctctg attcaaccgt gctctttatt gccgaagaat
     5581 accttgatag aatgaaaccg attgcaaaga aaatacctca gttaaaacat gtaatagtct
     5641 ggacaagaga cgggggtgag aaatttgata aacatggttt taattttaga aaaatgactt
     5701 cctacgcaaa gtttatgaag tcagccaaaa ctgccgagcc aaaagtagaa atcacattca
     5761 tggatcatgc aaggctcatg tacacatcag gaaccaccgg aagatcaaag ggtgttgtaa
     5821 gaccatgcgc cgcagactac agcagcgctt ataactacgc tcagataatg gacgttggcc
     5881 cggatgatgt ttgttttacg tgccttcctc tcttccattc aaacgccatg gtaatgagcg
     5941 tttacccggc acttataaaa ggctgtaaat cggtagtaga agaaaaatac agtgccagca
     6001 aattttggaa ctggataaaa gatcacgagg ttacaaaatt caatattgtg ggaacaatgt
     6061 catattttat gtggaactca ccagtagttc ctgaagaaaa gcagcacaag gctgagcttg
     6121 ttctcggttc acctgcacct cacgatatta ttaaagagtt tatgaaaaga tttaacataa
     6181 agtttatgga aggttacggt cttaccgaaa ttggtcagtg tacatggatg agacccgggg
     6241 agccatttag ggttggctcg tgcggcaagg aagccccggg gtatgagatc aagattgcag
     6301 accccgatac agatgaagag ctgccaaggg gtcaggtagg agagatcata gtacgcccaa
     6361 gaactcccaa tgtgatgctt cattactata acaagatgta tgaaaaaaca gtttctgact
     6421 tcagaaactt ctggtttcat actggcgatg ccggcaggat ggacaaggac gactacatct
     6481 attttgtaga cagggttaag gattatataa gaagaagggg tgaaaatata agctcattcg
     6541 agcttgaaaa aatagtgagc tcttatcccg atattaaaga gtctgctgca gttgctgtta
     6601 aagaagaaaa aggtaaatat gcagaagatg aagtaatgat cgtgattgta ccggaaaaag
     6661 gtaaaaagat agattttatt aagcttatga aatttttaga accgagaatg ccatatttta
     6721 tgattccgag gtttgtgaga atagacaaat cactacccaa aaccggtaca cagagggtac
     6781 agaaaaacaa acttagagaa aaagggatta ccaaagatac atgggacagg gaaaaagcgg
     6841 gttataaaat taagcgataa gagttttagt tttttgattc aagcaccaaa gccactgttt
     6901 gtgtcgcgat cttgccgtct tttataacaa aagtatccgt accaaaaggg attttgtaat
     6961 tctcagactc cgcactccag attaaatagg caatatcatc atttataatt tttttataaa
     7021 gctcaaaatc acatccgggc ggtactacat ctgctagaaa tttgttaaat aaagcggtaa
     7081 tctcatttaa attagtcagt attctttcag gagttatgag tgtagagctc tctgtatagt
     7141 ccagcatcag gccatctaca tctccctgct caaaagcttc caaatgatga tctataattt
     7201 tttgtgtttc agaactcatt ggtatttctc cattaacaat atttttatgc agatgaatat
     7261 ttaatatcaa gcggccttcc tacttcgggc tgccttactt tccatatcag accgtcgtat
     7321 atgaaatgaa ggaaagaact gcccacaata tagattaaaa aggcgttttg atcgagcttc
     7381 ataccaataa gactgagcac tactattact gctgaaaaag ctccgctgta tagccactgc
     7441 ttgtttgaaa ttttcttaaa aagcttttta ctgtcgtcta cttttttata cttagatgca
     7501 acaaatatat ttacaaatgc tatgtattct atggcatgag aaaagccaaa cactacgtaa
     7561 cctataatca ttgaatggaa gaatattgca taaatactga gcgtggagat aacaaaaata
     7621 ttttttggta tgctaatttg ttttctgttt ataaattctt gataaatata aatgagagta
     7681 aatatgatac ttacagcaat cagtatataa gaaagagatg ctatataccc catatagggt
     7741 gcggcatgac caaggataac tcttccagcg ttgtatttta atattgtact ctgctcataa
     7801 agaagcattg caaaaaaaag ataaaaaaac caagagtata atataccctt gtctacccat
     7861 ccttttccgt aacctgcttt cctggaataa atccttgtaa atccgtactt ttgcgcaaca
     7921 gaatgataca ttgtccaaag aacagaaagg gtgagtaagt aaggaaatac tttaaaatat
     7981 acagatagtg cagtagctac aactgcaatt accggaagat agatatatac ctgtcttctc
     8041 ttatcaaact cctctttttg accatataca agtgccatag tgaggtgtct gtgtacataa
     8101 ttaaatagca gagttattac aattattaac cctaaatgat tttggaaaat aagaagggca
     8161 agaaaaacca aaatccagcc aaaactaaaa taaatagagt ctgtaagcgg gtttttaatc
     8221 caaagcttgt tgtcaatagt cagtgtagac atgttctttg tatcttgttc gttataagga
     8281 ctgatgagtc aaaaccacaa aagaatttca aaattactaa ggttattatc aattaaaata
     8341 tatacttttc aaataaaaat taataaagat attcaaaaca aatattaata tataaatagt
     8401 gtgatagaat taattaaatt acaaattaat agatttactg caatctgaat aattaacaat
     8461 gaaacttgga tttttcacta tgcctctcca ccccccggga agagagcttc atgaaactct
     8521 gaatgatgat cttaagcaac taatattctt agacaagctc ggtttttctg aagcctggat
     8581 aggagaacac tttacagccg gatgggaaaa cattgcagcc cccgacctgc ttatagcaaa
     8641 agccctatcc cttactaaaa atattatact gggcacagga gtaagctgcc ttccggagca
     8701 cgacccgttt gtacttgccc acaggattgc tgttttggat catattgcca agggacgttt
     8761 ttattggggt attggtgcag gaagtttcat tggagatttc gatgcatttg gaatagatgt
     8821 caaaagcggt gagcaaagaa ctcttaccaa tgaatcactg gatttcatac ttaatctgtg
     8881 gaatgacccc aaaccgggcg actatcaaaa cagcaggtgg aaattccgcg tacccgagcc
     8941 gcaacaagac gtagcacttg gtgtacatac taagcctttt caaaaaccac accccccgat
     9001 aggagttgca ggaataacag agaaatccgg aacactgcag attgcgggag aaaaaggctg
     9061 gattccaatg agtataaact ttgccccagt ggcaacactt aaaacacact gggattctgt
     9121 acagcaaggg gcagaaaatg tgggtaaaac tgcagaccgt tccatgtggc gtatagcaag
     9181 ggatgtttat attgcagaga ccactgagca ggctagggaa gaagtaaaaa acgggactct
     9241 ggcaagagac tttacagact atttcttcaa aatggtccct aaaatccgcg gcaatttaaa
     9301 tatatttaag acagataaat caatgccaga tagtgatgtg acaatcgatt atatgataga
     9361 caacctatgg attgtaggaa gccccgaaga tgctgtaaaa cagatagcca gactgcatga
     9421 tgaagtagga ggatttggaa cacttctagt tatgggtcat gaatggaaac cagaggacaa
     9481 atggcaaaat tccatgagac ttctaaaaga agaggtaatt cccggactga aaaaacttgg
     9541 catataataa catttcagat ctttgctttt attaatcgat ttatcagtta tttaattata
     9601 aaatgggcca ctcaaaagga agactttttt taagagcatt aaagaataaa agtaatcaag
     9661 ttcagcaaaa acttaagtct cttggagttc ccgattatac aatctttgta atcctatcta
     9721 ttgtaaccgg agcagcatct ggcagtgtgg ctgttttctt tcataaattt atcgatttaa
     9781 ttgactactt tttattcgat tttattagat caagcgtgcc tgcattatct tctttcaccg
     9841 taattctttt ccctgctata ggcatgctct gtgtctattt gcttataaga cttgctccca
     9901 aggtttcaaa aagaaagggg gtaatggaag tgattaaagc agttgccaca aggggcgggc
     9961 atatcccttt tagaactact ctgtttaact ttatagctcc ttctatatgt attggctctg
    10021 gctgtaccct tggtcccgag ggccctgctg cacaaatagg cggaggaatt gcaagtaaac
    10081 ttggaaggat tttcgggctc tccgatacaa gaagaaggat gtttaccgca gccggagcag
    10141 gggctgcaat tgctgcggta tttagaactc ccatgggcgg agtatttttt gcgcttgaag
    10201 tagttttgtt aaatgatttt caggctccta tattctctgc tctaatactt gcatcagtaa
    10261 ctgcaagtgc catttcacag atgatgcttg gtgacaagcc gatattcttt tttgacaata
    10321 tcaacacagg gccttacagc gaattctatt tatatgcggt ttgcggaatt ctagcaggcg
    10381 ggttatccgt attattcatc aaatatgctg aatatataaa agaagtattt aaacaaaaaa
    10441 atattcagga aattccaaaa tggaaaatca tgggtggagt tggccttttg gttggtattt
    10501 gcggattatt ctttccggaa attttaggcg tgggatataa ggcaattaat ggtttcctat
    10561 cagacaccat cacatgggaa attgcacttg tgctttttgt gctcaagttt gttctggttc
    10621 ctatcgtatt atatgcgggt ggttttggag gtgtttttgc tccttctctt tttatgggtg
    10681 catcatttgg ttttgtattc tctatattct taaatcaggc gtttggattt aatctggata
    10741 caacggcttt tacccttgta tctatgggtg catttttggg agctgtaaat tcaataccaa
    10801 tttctgcaat actaattatt tttgaaatga ctcaggacta ttcatttatt ctgccactaa
    10861 tgctttcagt ggtttcaagc acaacgattg ctcagcttta ctttaaaggc tctatttaca
    10921 gtaagcactt ggaagaagaa ggctaccaca tcacacaggg gcatgacacc gatattctta
    10981 aatcattaag agtttctgat gtgatgagag atgatatagt cctgatccca gagaacctga
    11041 cactgcccac acttataagc aaactgggag agaccccaca cagcaccttc tatacaataa
    11101 accctgaagg aaaactcctt ggtacaatat atgaaagtga actgcgtccg gttctcacag
    11161 agtatgaaaa cctaagagag atgatagttg ccggagatat agcaaagccg gggataatcg
    11221 tagtaagaga aaacgatgat ctggattttg tattaaaact cttcggtcag gaaaatgtgg
    11281 acgaattccc ggttgtttca tctgaggact cttctaaagt cctgggatct gttaattatc
    11341 atgatattct ggctgcttat aacagggaaa cacttaagca ccaccttgcc gacgggcttg
    11401 ttcatgacct gaaagcaata gagaaattta catcagtaaa agtatcagaa ggctattcaa
    11461 tctccgaaca acttgcacca aaacaatttg taggaaaaac acttgcacag ttaaggttaa
    11521 gaaatgtgta tggggtagag gttttaatga tacgcccaca tcaccatcac ttcattgaag
    11581 aagaaaagaa gactgaattc attgttccaa gacatgatta tactatcgaa acaggtgata
    11641 tactcatagt ttttggaaat gatgagtcta ttggcaaatt aaaagagctg taatatttta
    11701 atgccaaaat atttaatgat attctaagct acatctcggt tgatggttaa gtccgtatcc
    11761 gtagtttttg gtttactgct ctcggtttta aattccgagg tgaaatgaaa ctcaatttct
    11821 ggaaatgttt tttgtttatc ttctaacatc caggcgtttt ctgccatata aaccagtctg
    11881 tcgtctttat ctttggtcac aagccttgag tacctgcgtg caaactcttc aagcgccttt
    11941 ttgtcttccg atgtgatcca gcaggcttta taaaaattta gatgttgaaa ttcgcaagct
    12001 gcgccatatt cgtgtagcag cctgaatttt attacttcaa actggagttc tcccactgtg
    12061 cctatgattt ttacattacc ctcttcacgc acaaaaagct gagccactcc ttcatctgta
    12121 agctgtctta ttcctttatc aagctgtttg gacttaaaag gatctcggtt tataagctgt
    12181 ctgaaaatct caggggaaaa acttgggatt cccttatatt gcagtacttc tccctcggtg
    12241 agcgtatccc ctattttaaa attgcctgtg tcataaagtc caaccacatc cccggggaat
    12301 gcctgttcaa ctatattctt gtcatttgcc ataaaactgg tgggattaga aaagcgaagc
    12361 tttttattta ttcttgtgtg atggtaatac acattcctct ctaaagttcc tgaaactatg
    12421 cggcaaaaag caaccctgtc cctatggttg ggatctaggt tagcgtgtat cttgaatata
    12481 aaacctgtaa atttgttttc tgaaggttct acaagtcttt tatctgccat acgcgtctga
    12541 ggttcattag caattcttac aaatgtatcc agtagttcct ttaccccaaa gttattaatg
    12601 gcacttccga aaaacaccgg agctaaataa ccgtctctgt ataatccttc atcataatct
    12661 tcataaacac cgcttataag ctctatatct tctctaagct tagcagcatt ggaatctcct
    12721 atctgctcat caagcttagt actatctata tctgtaattt caacatggct gtcactttgt
    12781 ttagtttttt ctgattcaaa cagctggagt ttattttcat gaatattaaa aacacctttg
    12841 aaatttttac ccatacctat cggccaggaa agtggcctta catgaatatc gagctctttt
    12901 tcaatttcgt ctagcagctc aaaagggtct tgcccttccc tgtccagttt gtttataaaa
    12961 ataattacgg gtgtatgacg catcctgcac acttccataa gctttctggt ctgctcttcc
    13021 actcccttta cgctgtctat cacaagaatc acactgtcca cagctgtaag ggtacggtac
    13081 gtgtcttcgg caaagtcttt atgaccggga gtgtccagaa tatttatctt atagccatca
    13141 tattcaaaac ccatcacaga agttgccaca gaaattccgc gctgtttttc aatttccata
    13201 aagtccgatg tagcagactt ctttatcttg ttgctcttca ccgcccctgc tgtctgaata
    13261 gctccgccaa agagcagtaa tttctcagta agcgtagtct tcccggcatc agggtgactg
    13321 atgattccaa atgttcttct tttacttatc tgttcttgca gtttcatgat taatcttgtt
    13381 acaatgtaaa ttagcgcacc agtttaccgg attaaaataa ataagcgaca aattatatga
    13441 gtatataaat attcacattt tattaatata tattattttt tatcgtatta agctataatt
    13501 accattactt attatttaaa tttaactgaa taatttatag agataatact aatgagcact
    13561 gctaaagatg aaatcaaagc tcttttggaa aatttgcctg aggattgtac atttgaagat
    13621 gttcaatacc atttatatgt ggttgaaaaa attcaaaatg gaatattgag agcagatcaa
    13681 gaaggaacta tatctcagtc agaagtggaa aggaagtttg agaagtggac aagcaagtaa
    13741 tatggtcccc cgaagccgta gatgaccttc attcaatcgt agaatttatc tcaaaagatt
    13801 ctgaatatta ttcaagcgca gtcactacaa agattttaac cacaacaaaa caaattggca
    13861 atttcccact tagtggaaga atagtccctg aattaaataa tgagaagata agagaacgtc
    13921 tggtatatag ttatagagta acgtatataa ttagagaaga gtctgttctt attactgcaa
    13981 taattcatgg taaaagacta atagagaata ttgaagatag gattaaagac acttaaacaa
    14041 tcttatcagt atattcatta agttcattca gttattcgaa aagacttatt aagtgactaa
    14101 acaatcacat ataaattaat taaacaataa tcctcatata taataatatg aactatgaat
    14161 gagataacag tatcaagctg gaatgaactg caaaccgaac tttttaccga atcatggaac
    14221 gaaaagcttg gcagattccg ttccagatac gcatttaggg ggctttctga ccgtgaatat
    14281 aagcttgaat cacggctcta cagacacggc ggagatttta aaaagctgga aaaacacctt
    14341 ctcagaaatt ttaaaaaata tgcacataga gatgtagttg aaaaagactc tatctggaac
    14401 tggctttccg tagcccaaca ttatggtctt cctacaaggc ttatggactg gacatactca
    14461 ccatttatag ccatgcactt tgctactgca aatcttgata agtttcatac cgatggagtt
    14521 atatgggcgc taaactatgt acacacacat aaccttctcc caaataaact aagaaagaaa
    14581 cttgatgaag aaggtgccaa tgtttttact gttgaaatgc tgtttgaatc tattaagtcc
    14641 atctctgaac ttgaacaatt atccaaaaag ccttttgtaa tgttttttga gcccccttca
    14701 atcgatgaca gaattgtaaa ccagtttgca tttttttccg tatcctctca ttctaacatt
    14761 gtgctgaata catggctcga ggaaaaacag gatatctggc gtaggataat tatccccgct
    14821 aggctgaaat gggaaataag agataagcta gatcaggcta atattacaga acgtgtgctg
    14881 tttccgggac tagatggtct tagtaagtgg cttgccagac actatagccc aaaagtctag
    14941 aattgaatct ttatcacttt catcattctc taattaaatc atacttgcaa attctatgaa
    15001 tatttgctca aatatgaatt agattattta ctgctttatt ataatgttca taaacacagg
    15061 gggataatat ggaattaaat gaaaaacaat taaaaatgtg ctcggagagc aaatgggact
    15121 tttctgactt aaaagcttta tttcttaatt gtacactaaa aaaatcacct gaagtttctc
    15181 atactcaggg gcttatcgat atttgtaaag ccataatgga aaaaaataaa atctccgttg
    15241 aagtattaag gcccatagat ttcaatcttg cctttggtgt atatcccgat atgaccgaga
    15301 aaggctggga cgttgatgac tggccgcaga tttatgaaaa agtgaaagct gcacatattc
    15361 ttgtaataac aagccccatt tggcttggag aaaaatcttc agtatgcacg cagacaatcg
    15421 agagactata ttcatcctct ggcgacctta atgaaagtgg gcagtatgcc tattacggaa
    15481 gagtcggtgg atgcttaatt acaggtaatg aagatggcgc caagcactgc tcgatgaaca
    15541 tactttactc tctacagcat cttggatatg taatccctcc tcaggcagat gcaggctggc
    15601 ttggtgaagc aggccccggg ccttcatatc ttgatgaagg atcaggagga cctgaaaatg
    15661 attttacaaa caggaataca acatttatga cctggaatct aatacacatg gcacgtatga
    15721 taaaagatgc aggcggaatt cctgctcatg gtaatcagag atcggaatgg gatgccggct
    15781 gcaggtttga ttatccaaat ccggattaca ggtaaatcat attaattgaa tacatataag
    15841 tattaggtat cttttacgcc caataatcag gcatgttatg gtaaacattc tattagtagg
    15901 acttggcggg tttattggtt ctgtatcaag atatatgttg ggtgatcttg ttcacagact
    15961 tttggacaaa cctgtattcc catatggaac atttgttgtg aatatctttg gttgtcttct
    16021 tataggtatt ctcggaggac tttctgaaag caagaacctg ttcgatacgc aaactcgaat
    16081 gtttcttttt attgggcttc tgggcggatt cactactttc tcctcatttg gttttgaaac
    16141 ttttaacctt gccaaacagg gcgcacattt tcatgcccta acaaatgtat ttctacaaat
    16201 tgtcttagga ctcatagcag tatctattgg atatattgca gcaaaaaata tttaatttac
    16261 tctttatttt agtttttaaa gatatctctt ataattaatt ccatattatc ttatttagga
    16321 gaatactatg tatcgaaata tgcagattgc attaataatt tcagtaatgt tattttcaat
    16381 tagctgtgcc ggtaaaaaca tgttcacaga gggacaatgg attgatttaa cacatgagtt
    16441 ttcagagcaa actatttact ggccgacttc ttcgacattt aaaaaagaac cggtttatga
    16501 aggtcatacg gaaaagggtt attactactc tgcttacaag ttcagcactg ccgagcatgg
    16561 aggcacacac attgattcac caattcattt ttatgaagac agaaacacag ttgatcaaat
    16621 acctattgaa cagttaatcg gtcccggagt cgtagttgat gtatcaaagt atgcattaac
    16681 taaccctgat taccaggtga tggttacaga cattctaaaa tgggaaagtg ctaacgggca
    16741 aataccagat ggggcgatat tactattaaa tacaggctac gggaagtttt ggcccgacag
    16801 agttaaatat atgggaactg atcagttggg gcaggatgct gtaaaagatt tgcactttcc
    16861 gggtctgcat cctgacactg ccaagtggct tgttaagaat agaaagataa atgcagtcgg
    16921 acttgatacc cctagtatag attacggcca gtcaacccat tatggcagcc acgtaatttt
    16981 atttgaggca aatattccgg cttttgaaaa tgtagccaat ttggacaaac tgccggcaac
    17041 aggtgcaaca gtatttgctc tacctatgaa aataaaaggg ggcagtggca gcccgcttag
    17101 aattatagct aatttttaat gaccttatta caatttatat gttaaaataa actggatcac
    17161 agattataag gaggctttat atgagcgcag aagcaatgga acacattggc gagagccagg
    17221 gtatggaaaa ccatgatcat gatatgattc atgatttaag tctcagatta gatgctctat
    17281 ggagatatga tcagtatata gcaaatgcaa agggcaacag atctttaata gcattttgga
    17341 aaaaaataaa agatcaggaa agttctaata tccaaagcct taaaaaacac attgcagccc
    17401 atgcaaaaaa aggctgcttc taatttaata gactggtttt caattagatt attttacttc
    17461 aaattttaca attaatcctt aatatcggaa tatttatttc ggtttaagga atgctatatt
    17521 gataaatatg aatagaaaat cactgctctt actttcttca ttattttttt gcatatttac
    17581 cctgtccggc tgtgaaatga tttctggttt cttaaaccct ttttctggaa gctggaaatc
    17641 cggccttttt catttcacat tcaatacaga caactcattc aagcttgaag tgggaagtgg
    17701 attgtcattt aaaactgaag gaacatataa atatgacaaa gaacaaatta cattaagctt
    17761 ttcaaaagat agcaacacaa cattcaccta tgtgttcaat gaaaataaat cagaattaac
    17821 cctcactcct aaaacagaat tcaaatggtt taagaccgcg gttaaattta ctaagtctaa
    17881 ttaaaagctt taccaattta tttccaaatt atttgatata atgttgtata aatctttaac
    17941 ttttgaggtg aattatgtcc aatcacatac atgacaaaag ttttccaggc gaaagcaaag
    18001 aatatcgcag tgcaaggaac gagctattaa aagccgagat tgaacttagg cagagaatcg
    18061 aagaagtagg caggatgaaa caggcactac ctttgggtgg gaagcttaaa aaagactatg
    18121 ttttcaaaga actaattaat aatgaagaac gggaaataaa actctctgaa ttgtttaaag
    18181 acaataagaa atctcttatt atatatagct atatgtacgg gcccaaaatg gaaaatccat
    18241 gcccggcttg cacctcacta attgacggtt ttaacggttc cgctatgcat ataactgaca
    18301 gggtaaatct agctgtaatt gctaagtccc ctattaagag aatagtagag tttgcagatt
    18361 ctagaaattg gaaaaatata catctgcttt catctgaaaa tacgagctat aatgctgatt
    18421 attttgctga aacctccgag ggtaatcaga ttccagcctg taatgtattt acaaaaactc
    18481 ctgatgggat atttcactat tatgcagcag aactactata tgctccactc gatggacacc
    18541 caagacacat ggaccttatg tggcctgtat ggaatttgct tgatattacc cctgaaggac
    18601 ggggtacaga ctggcatccc aaattatctt atgacaaata gcatttaacc tgaattaaag
    18661 actggaattt aatttttaaa tattatacaa ttatatatag cttgttctgt attgaggaac
    18721 atatgatggg ataaataatc ataatctaga taattgtaat atttcttact taccaaagat
    18781 attttttaat tttattaatc tctgtaaatt ttattgtcca tatgggattt actctcggct
    18841 tgatcacaca tccttagaaa acaatgtctt atttgatata aacggttaat agtcaggagg
    18901 ggtattatgg cagattcaaa cgggaaggca tttttaaagt atataggaaa aagaaaatgg
    18961 agaagagtta aaagcacaaa gaattctcct gaaaaaaatt tccttactta tcttggaaat
    19021 agaaagtgga agaaggctaa atcttagttc aatacaaaaa ttcttttgat gtattcttac
    19081 aatcattgag tattgaatat actaaatatt gaaccttaat aggtacagtt ctatcttatt
    19141 taagtacagt attaagccct gcactatata aaaactaaaa aatctttaaa aatcatttca
    19201 attggatgta taattatatt gcttgttgga attattaatt cataattaca tatttagaaa
    19261 ttattttacc attatttgtt tagtagcatc aggatttata taaatgaaaa acgatttgcc
    19321 tgacaaacag caaaatgaaa acttaaaaga taaagattca cgcatatcgg aactggaaaa
    19381 gatattagag ataagtaaag agcttacttc ttctctaaga ctcgatccac tgcttaaaaa
    19441 aattgtagaa acagcgtccg aactcacaga tagtgaagga gctactatat tcctgctcga
    19501 tccaaaagac ggaaacctct acttcagggc aactactgcg caatcttcaa acttactttc
    19561 agaagttccg gtaccgcttg atgactcaat tgcaggacag gtacataaca caaaaaaacc
    19621 gcttattgta gatgatgttc agtccgaccc gcatttttat aagggtattg accagatact
    19681 tggatttaaa tcgaattcac ttataggggt gcctcttata ataaaagagc gcagtatagg
    19741 agtattggaa ggggttaata aaaaagggca aaaggaattt acgcaaaaag atgtcgatac
    19801 actcgtggta ctggcttcgc atgcagccat tgcaatcgaa aatgcacggt tatatgaatc
    19861 agtagtaaac caggctgaaa ttctcgaaca acaggttgaa gaaagaaccg ctacactcaa
    19921 ggcaagaaat aaagaactta ccgcttatga tcagacagtt gcacatgacc tcaaaaatat
    19981 acttactcac atcattatat atgctgaaaa cctaaatgaa ttcagcgatg tagtaaatga
    20041 tgaagaattc gattctaacc tggacaacat atccaatatg tcttttaaga tgaacaatat
    20101 tattgaagaa ttgctgctac tatcaggtgt tagaaataca gaggtacaaa caaatccagt
    20161 tgatatgtct aaaataatta ccgaagcaca gaagcggcta tcattgatga tagaagaaaa
    20221 aaatgccgag attaaagcct cacagagctt accatcagca atgggatatg ctccgtggat
    20281 agaagaagtc tggtttaatt atttgagcaa tgccctaaaa tacggcggcg atccacctgt
    20341 tatcgagata ggatcaaaag aagaaccaga cggaaatata tcatttttga taaaagacaa
    20401 tgggcagggt atacctgtag acaagcaaga tgatattttt gtaaggttta caagctcaaa
    20461 aaaagatttt tccatgggcc atggtctggg actatctatt gtaaaacgta ttgtcgataa
    20521 gcttggcggt gaggttggag tacaaagtga gcagggtaca ggaagcacat ttttctttaa
    20581 acttcccaaa gcccagtcac tgaactaact tgctgacgct tcttttgata ttttgtttaa
    20641 cattccggta aaaattatgg cgtggagtgg gtaaagagca taccagtaaa gtagccctcc
    20701 aagtcccatt ggatcaaaga tagccgtctg aatgatatag gtttttcctt caattttctt
    20761 tgcttcaagc tgaagccatg ctctacccgg aagtttcatc tctgcaaaaa gcctaagaag
    20821 cttatttggc tcataatctt caactctcca ccagtctact acatcgccta ctgctatgtc
    20881 ttccgggtct cttctgcccc ttctaacacc tactccgcca actaacaaat caataaaacc
    20941 cctaaacctc caaagccagt ccgcatagta ccatccggtt tggcctccaa ttcttctaat
    21001 cggcaagaat gctcttttta cagaagcgtt tacctctttt cttcgggtat caattattct
    21061 ggtaccaaat tttatcccac cccaatttct aagcgtatct cctgatgaaa gtgaatcaga
    21121 ccacctgttt tcataaaatt gtttttcttc ttctgccaga gcttctttta ttgcatcact
    21181 aactgtaagg gtctctatat caaaatgatc ttttgccaga tgatcattaa taactgtggg
    21241 gtttttaaga ctatcgatga tctttcttcc aatccttgca tatacaggcg taacaagacc
    21301 aagccacagg ctcgataacc atggagttag aaccggaacc ggaataatat aacgttttaa
    21361 gcctctttgc cttccgtatt cctgcataag ctctccatat gttactacat ctttacaccc
    21421 tatctcaaaa atctgattac ccttaacttt aagtgttatt gcctttgtta aataattcag
    21481 taaatctgat attgctatgg gttgagtatc cacatacacc cattttggcg tagtcataac
    21541 aggtagtttt tcaacaagtg ctcttataat ttcaaatgag agacttccgg atcctatcac
    21601 tacagacgcc ctgaactcga tcacctgcac tttcccactt ctaagaactt gccctgtttc
    21661 gtgtctgctt ttaagatgag gagataaaac cttactagaa tctcccaggc ctccaagata
    21721 tatgattctc cttatacctg cattttctgc agccttaaca aagttttctg caccttgccg
    21781 gtccttatcc tcaaatccat cctttgaacc catagagtga atcagataaa acgccgtatc
    21841 aatacctact aaggcctttt ccaatgactc tatatcaagg acatcgcctt ttattatttt
    21901 tacagtattg ggaactttgg atttcaggta ctccggtttt cttgccaggc acttaatatc
    21961 atattcgtta ctttcttgca gggttttaag taatctgcct cctatgtacc ccgatgcacc
    22021 cgtaattaga atagtctttt taacctttat tttgttcata atgtgtccag aacttctaat
    22081 ataataagta ataatacccc taaatcttaa tttcggttat tttaataagc ttttgtggat
    22141 atatatttta ttttagaaga aaatatatct tgttttctct gaacaacctg tattataatt
    22201 aaattaaatt tagagagttt aaattatgaa tactgtaagc gatatgctac aagagaaagg
    22261 aaccggactt tgcacgatct caccaaatgc cacagtatat gaaggccttc agataatggc
    22321 cgaaaaggac gttggcgcag tgttagtgac tcaaaacaac aaactactgg gtatttttac
    22381 tgaaagagat tacgcaagaa aagtaatact gcagggcaaa tcatccctaa acactgcaat
    22441 aaaggaatta atggtcaagg atgtattgat tgtaaaacct tcagattcaa tagaccagtg
    22501 tatggcagtc atgactgaaa ggcagataag acacctgcct gtaatgcagg gagatgaact
    22561 tcttggtctt gtttccatga gagatgtggt aaaccatatt atctcacata aaaactttaa
    22621 aattaaagag ctagagaagt atataaccgg ctcttattga tttttatata tacaatcttt
    22681 ttaacatttc agcagccgtt tcatcaagct gttcccttct tctcacatcc tcaaatcttt
    22741 catatagttc ttttggacga agccttttct tttctgctcc cctgaaatca tggatcactc
    22801 tgccgccatg catcataatt aacctgtctc caagagctac tgcctgctgc attgaatgag
    22861 taaccatgag tgtggttaga ttctctttac ttactaattc ttcagtaagt ttaataacct
    22921 gatttgcagt cttaggatca agtgccgcgg tgtgttcgtc aagaagaagc aattctggtc
    22981 tttgccaggt tgccataaga agggtaagtg cctgcctctg tccacccgaa agtcttccta
    23041 taatattatc aagcctgccc tcaagtccca tgttaagagt ggatactcgt tgtctgaaac
    23101 tctcaaatac ttcacttttt agtgccatat caagcccgcg actctggcca cgttttgaag
    23161 caagagcaag gttctcagca atagtcatat ttggagctgt accgctgaat gggttttgaa
    23221 atactctgcc gatcagattg gctcttttat gctccggcca ccttgttatg tcatttcctg
    23281 atagctcgat acttccagag tctgtcatca cattgcctgc aactgcattt aaaagagttg
    23341 atttacctga accatttgta cctataacaa cgacccacga tccttgttct atagaaaggt
    23401 ctacgcccca aagcgcctta acctcattta aggtatctgg gttaaacgtt atagatatat
    23461 tgtttatatt aagtattttt gactgtgaca ttttttttat taaactttga tatcagactt
    23521 ggaagaacta gggcaataaa tacaaatatt gccgtaatta atttaagatc attggggttt
    23581 acccccactc ttagtgcaat agctacaaga agcctaaata gtacagatcc gataacagcc
    23641 ccagctatgg taaaacccag gctgggcgtt cccacaaggg cctgtcctat tattatgctt
    23701 gcaagacccc atataatcat tccaattccc atctgaacgt ctgcaaatcc ctgaaactga
    23761 gcaagcagtg accccgcaag ggcaatcaaa ccgtttgcaa gggcaatacc tagtatagtc
    23821 atattttcta catttacccc cagtgctgtg atcatctgag gattatcccc cgtagcccgc
    23881 atagcagtgc caatatttgt tttaaaaaac agatatacca cacaacctac aacagcaatt
    23941 aaaattaaca tggcgaataa aacggataga tcgcttgtgc ttacatccca gccaagaaca
    24001 tccactaccg ctgactctcc aaacaattta tgccagatgt tttctactgt ggtaactagg
    24061 ctctttgtgt ttatgagcgg gatattactc ttacccataa tatgaaggtt caccgaatat
    24121 agtgaagtca tcactaaaat tcccgaaagc agattattta ttttgaattt tgtatatagt
    24181 acgcctgtta cggtgcctgc taacataccg cagataaaac caaaacctgt cgcaagaagg
    24241 ggtgagtacc cgtttacaag caataaggcc gttacagcgg cgcctgtagt tattgatccg
    24301 tctgtagtaa tatcggggaa atcaaaaata cgaaaactga tgtagactcc caatgcaagg
    24361 agtgacagaa ttaaaccaat tatcagtgcg ccaattaata aactcattta gtttatgcct
    24421 ttattacaga ctctatggga cttacccaga gtcctcccct aacaaattca gattcccctg
    24481 ctccaacgat atccctttca tctgagagca gatgaataac agattgaagg gactgcctat
    24541 caaataaatt cttgatagtt tcttttaaat ccagttttcc cttcataaac ggctcataag
    24601 aaaatatcat cgcatgaaaa tgaccaaata gacccgtgct tcctaacggt cttagaaact
    24661 ctcccaaatc gccagactgc tcttttgatg ctacaccaaa aataactaca agcgatccga
    24721 tataggattt ttcagatgta aatgaaatac agttttgtat gtttggaaac gaaaaaatgt
    24781 catcaattcc tgattcatct gctacaggtg atttaattag attggcacca actattccag
    24841 ccgattctgc tactgctgca atagcaatag tatcttttcc tgatatatta aatcccgctt
    24901 ctatcaattc actaaagcca ataacttctt tctcctttgc aggatcaaat cttgtcatac
    24961 tgccaaattc acctttgcac gtaatgctgt aaagagcatt tacctcgggc aaataatccc
    25021 ctgatgcaat atgaaaatca gcaacatttg taccatctgt tggaagataa gatacagctc
    25081 ctgcaagtgc agcaaactcg ccgtatctgt ttttacaatc atcataccca tttccaaatg
    25141 cccctatgcc aagagcataa caatctccag atatctgtat tgggatattt attatctttt
    25201 tattattatg atctataaat tcaatacttc ctttaatttc acagctcaga ctgctatttt
    25261 catcttcgtg taatatttca aatactccat tttcagtatt gattctttta gtttcagcac
    25321 cctttgtaat actagtagta tcttcagttt tatgtggctt aagaagtgct gccactcctg
    25381 agagctctaa tacagactgt acgttctgag agatattaac cagactaaac tgcccgttta
    25441 tctggtttaa ctttttgtag gattgcagaa ggattcttat gccggcagag ctaagatagt
    25501 taacacccgc catatcgagc cttattttat agtggccctc tcttatggat ttagacagct
    25561 cactttctag atgatcagcc caatacgcat cgagccttcc atctaaaatc agtatctcga
    25621 atccttcttg atgttgtctc ttaatttcca ttatcttaaa accgtctttg caccagcatt
    25681 aagtgactcg ggtatatgta gatttgatat tttggcagca tccaggttca aaatcaaact
    25741 ggtttcttca agtcctacaa aaggcatgtc ttttggattt tcccctctca tcacccttac
    25801 tgcaagcttt gccgcttctt ttccgaagtc atagtagtcc tttgcaagaa ctatcgatgc
    25861 cccctcagca gactgaactg tttggaaagc aaatatcggc agtttggatt gattagcagc
    25921 ctttgcaata cttgtaaatg ctaccgctgt gagatttccg ggaatttgaa taatggcatc
    25981 tatatcgctt gctgccattg ctagtgctgc atccggaact tcagtagaag tattagccgg
    26041 caaagaaaca agttcaactc cgtacttctt ggcctcaacc ccgagcagtt tattgtggag
    26101 aactgaatta gattcagcag ggacaaagag cgttcctatt ttttttgcat gcggcataag
    26161 ttttttaaaa agcgccatca tttcaccata ggcaggaagc gcataaacac ccgtaatatt
    26221 aggcagatga tcttcattgg tcctgcctgc acctgcatta accccgcttg cacaatatgt
    26281 aaacactacc ggaatattct ttgccctttg tatggcagcc tgtagtgtag gtgttgaaag
    26341 tgtcataagc atatctgctc cttgtgatag agcgttatcg atcattaaac taactgtagg
    26401 catatctccc tgtgcatttt ggatttttat attatagtct ctgccttctt taagacctgc
    26461 atgttgcagc ccttccaaga taccctttct tgtttcttct acatccgata cttctataaa
    26521 ttccactacg ttaatattcc atcttttggt aagtctaatt ttttcctctg agtcattttc
    26581 tacagtattt acctttctta tttcttttcc attttcatct atcactacat cgacactttt
    26641 tagaatatct tcggggattc tccagttatc ttttagactt tcaatagcct gtttattaat
    26701 gaccactacc tcgggagcca gatttcgaat tggaatagat tccggatctt caccgttcag
    26761 tacccttgct gcaaggtatc cctgtctctt accaacctca taatagtctg ctccgatatc
    26821 aagtaatgtt cctttattgg gaaaagtagg ggcattggta ataaccggga ttttcccgtt
    26881 ttttgctgcc tgcactacat tatcaagtgc tgtaatgact gtgttatcac ctgatacaag
    26941 aagcgcatct actcccctgg ataccagaga tgccgcagtc tcgtatacaa ccgatgtgtt
    27001 ttcaacagta gcttctaaaa gctcaacgcc cacgagcttt gaataattac gggcctgtat
    27061 tgtacaaact acagaattgg cctcagcaga gttccataac attccaatag attttatatt
    27121 tggatttaat ttcattgcag tttctataag tttttctaca ggctgctggg tgccgtatcc
    27181 cacaatatac tcggggtggt cttcgggttt tgctctatcc agccctatac ccgatgcaaa
    27241 cggatcagtt acaaggccaa atacatgaac cacctttccc tgcttgttgg cgtttgaaac
    27301 tgcctgcaga gcaggtgtgc tcactgttaa agcataatca aatttaccgc ttgttatttc
    27361 actggctatg gtatttagag tcggaatatc accttctgca ttataaaact gtatgtcgat
    27421 attctgacca tcaataaagc ccttttcttt tagcccgtcc aatatccccc ttataccatc
    27481 ttcaagtatg atatggcttg aaagctgcag cattgcgatt ttcggaatct cggattttgt
    27541 acgttttctt tgattaagat cagaaaggag caggactgaa cttgcaagaa ctatcaggaa
    27601 tatgcttaag gagagtcttt tcacttttat gttttatttc ataggttata taatccacag
    27661 attatatcag agcagatttt tgttatcaaa atagctgtaa actattttgt tctaatctct
    27721 tttccttttt catcaattat tacatcagct ttttgaatta atttatcggt aaatttccag
    27781 gggtctttca gattcctaag tacttgttta ttaattataa gtacttccgg gactacattt
    27841 tcaatgggaa tagtcgccgg atctttacca ttaataatgc ttgataacag atatccctgg
    27901 agttttccta cctgatagaa atctgctccc agatcaagaa gagtaccttt gttgggaaat
    27961 gtaggaatga gagttaaaac cgggattttc cccttacttg cagcatcaac tacattatcc
    28021 aaggctaatc ccactgtgtt gtctcctgtt actagtatca tgtcaacccc tctggaaacc
    28081 agagaattaa ctgcttcatt tactgccact gatgaatcta caatgacttc aacaagcttc
    28141 aaattttcct gcttcgtaaa ttctcttgct agaatggtat atgcctcgga gtttgcctca
    28201 gctgcattcc atgcaagccc tattgacctc aaatcaggat aaagctcctt tgcaatttta
    28261 attagtttaa ttacaggcag aaaagaaccg taccctacga ggtgcttcgg atgatcaagc
    28321 ggatcattct tctttagacc tacccctgct ataaaaggat cagtaactac gccgaatatt
    28381 tgaataactt tgccctgctt gtttgcattt gcaaccgcct gtagtgcagg cgtgctcact
    28441 gtgagcgcat agtcgtattt accacttgta atttcacttg ctatagcgtt tgcagtaggc
    28501 aggtctccct cggagttaaa atgtttgata tgaatattaa tcccgtcttc aaatccgctc
    28561 tctttaagcc cgtcaaatat tccctccact ccatcgtcca aaaaagtgtg tgatgaaaat
    28621 tgcaatatag cgactttcgg tatagtcctt tcattcttct ttctctgatt aaggtcagag
    28681 agaagcagga ccaaagaagc aagcaggatt aaaaatatac tgaagtatag tcttttcaat
    28741 atggtttatc catatatgga tagttgattt tttaatagat tatatcagaa atgcatgtta
    28801 tctacgacca tatatatctt atttattcac taagttcgat acagtataaa atcaagttcg
    28861 aattatgatt atttttggtt ttcaggactt ttgtatgaaa ttgcttgcca gggatattta
    28921 ttgcttgacc ttttataatt agaaggcgta caatttgcta tatgtagtaa attcagatta
    28981 cgaagggggt taaagtaatg tcttattcgg tagaaggaca gcttttagag gtatgtaact
    29041 gtaatatact atgcccatgc tgggtcgggg gtgatccgga tgaaggctac tgtgaggcaa
    29101 tagtaggctg gcatattaaa gaaggtgaaa ttgaaggagt cgatgtaagc gggcgtacgc
    29161 ttgccatgct tacccatatt cccggaaatg ttctggatgg aaactggaaa gtaagacttt
    29221 atgtggatca ggatactaca gatgctcagc aggagggaat actaaatgtc tggggcggca
    29281 aactaggcgg agcaattact gaccttgcag gtcttgtagg tgaagtagtg tctgtagaaa
    29341 aaacaaacat tacatttgat gtagaaggcg taaatggaac aataaaagta ggggatgttg
    29401 cacaagcctc attagaagca tttaagggag gcaccggatt agaaactact ctgcatgatt
    29461 ctatattcag cacaatacca ggcaaacctg cttatgtggg taaggcatca agctttaagg
    29521 ctaatgctcc gggttttgat attgatctgc aggaccataa cgcaatctgc ggacatttta
    29581 aatttgaggc atagtactat ataataaagc attccaatta gctttattgg gtttctatgt
    29641 cgatttttta taaagcatca ttctctccaa gggttgagcg aggaattctc ataactgtgc
    29701 tatccgtact tgcactctta tgctggattg ttttgtggaa aggtggtgat ctagcgcatg
    29761 tagcacacaa cttcggtact caactacaca atcatcactc gggccaccac tctgcaaact
    29821 catacagttt tatggtaatt ttcatagcca gctggtcggt tatgaccgtt gccatgatgc
    29881 tgcctacaac tatacccata gtagcctttt ttcataaatt tactgaaaac cgagcagaca
    29941 gagtcttact acttagtctt ttacttttgg gttatgtggg cacatggaca gtttttggag
    30001 tcttgatcta ttttcagata gctcttataa aatgggcgct tgcttcattt tctttcttat
    30061 cagactacat gtggactggt cctccactta tactcatttt tgcaggcctc tttcagttta
    30121 cagaaatcaa atatagatgc ctcgataaat gcagatcccc gcttagtttt attacggagc
    30181 gctggagaga tacaaaagag aagtggaatg cctttatgct cggagtagat cacggcaaat
    30241 tctgtatagg ctgctgctgg gcgcttatgc tccttatgtt tgccgttggg tttggaagcc
    30301 ttatatggat gctagtgctt gctcttgtta tgggtattga aaaaaatgtt aagtggggaa
    30361 gaaaaattgg aaagccagtg ggttacatac ttgtagtttc cggactcttt atgctggtat
    30421 atacttatgc caaaatctat taattatgcc gacttattta taataaactt ctataaggtc
    30481 aaaatgaaag tccagtttgt gtgcagtatt attttctttc tccgtatatc ctatttctaa
    30541 cgttagctct tttgtatccc gggaaatttt gttgaagaaa atatatcctg acaaagtcgc
    30601 cccgggtctt ataactccgg tacttaatgc ttttgcatat atatcttcta aatcaggctc
    30661 ataatcctgt cttctgtaat aaggataggg ataatcatca tagtaataat aaggcctgta
    30721 aaagggatga tggtggtgtc cataataatg aaacctgtcg tgatgaaatg aagtgcctat
    30781 gccaatggaa attcggggat atatcctaaa acgtttttgt tcggctgatt tcactatgtt
    30841 agcagccctc tcaggcgata gggcattaaa ttgatttctg ttttcatcta acaaaatgaa
    30901 gtcggcctct gagataataa tatttttatc tgtgttgttt tgaacttcga taaaaaatgg
    30961 aattaaatgc ctcctgagcc tctttggttc acctttccat acatttgcag aaatactaaa
    31021 attcacctta tttgcaagct ttggcgtaga gatgttctgt ggatcaatct tgatataagt
    31081 tctattagcc cttaaaccat atgcgcatcc acttgtgagg ataacaacac ttaaaagaac
    31141 agcataaact gagagaaagc taattttatt catcaattca ttcatattac aaatatatta
    31201 accgaataat tcaaataagg attcaaaaac taaaactttt tataacttgt ctcgtagtag
//
LOCUS       DBNGSV010000346         6465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1725563, whole genome shotgun sequence.
ACCESSION   DBNGSV010000346 DBNGSV010000000
VERSION     DBNGSV010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1725563"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC159U_12295"
     CDS             <1..540
                     /locus_tag="AC159U_12295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR3337876.1"
                     /translation="LATGTSDVSINFIDHNLASKAVGLDPSINMLLESKKKLKNKQLS
                     NKISIVRGIAEDLPFTEGLFDAVTISFGIRNTVDPQKSLNEMYRVLNKTGSIGILEFS
                     LPKNKIFGPVYMAYLNNFFPLIGSIFGASEEYRYLGDSISKFPNRDNFINLMKNAGFK
                     NCRYEELNIGTVIMYRGFK"
     gene            complement(542..988)
                     /locus_tag="AC159U_12300"
     CDS             complement(542..988)
                     /locus_tag="AC159U_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337877.1"
                     /translation="MVAATPDNLAHKETEIIYKNNQKVTSIPFNRGHCELALILKDAG
                     LKWRPTVGHYIWDPEKKILKKSPFNNRIYYVLNIEHFIKIFSSIDTVKKLFIWLPTYY
                     HSRLICEELGIEKSLIFEKILSANVKSTADELSVLYNLIITKLNSV"
     gene            complement(1159..1650)
                     /locus_tag="AC159U_12305"
     CDS             complement(1159..1650)
                     /locus_tag="AC159U_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006069199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR3337878.1"
                     /translation="MNNLKVLTVLIVTMSLLGMNALAGKYEKKDIVDTAVSAGSFKTL
                     VAALQAADLVDTLKGEGPFTVFAPTDEAFAKLPEGTLEDLLKPENKEKLVAILTYHVV
                     AGKVTSDQVVGIKEAQTVNGKSLAVDVKDGKVMVDNATVVGTDVMASNGVIHVIDTVV
                     IPK"
     gene            complement(1841..3163)
                     /locus_tag="AC159U_12310"
     CDS             complement(1841..3163)
                     /locus_tag="AC159U_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3337879.1"
                     /translation="MPNRAIFWFRRDLRIKDNIGLYHAVKESDEVIPVFVFEDKILKT
                     IKQDNQRFGFLVDALENLNKQLKKIGSYLHIARGDTRKELTKIIKKHNVSALYFNKAH
                     SLGSIEQEKEVEKKCEQLNVEVRKYSDSLIVPIEKVPVRKVFTPFYRLWQKEVEMCKP
                     LNIKKINSPKIKPKIISETIKDLKYCSNKNWPISFISQRAKEFDFCDYVNRRNFPYID
                     GSSKLSPYIRFGIISIRQLYNYVSTLGCPDDTYISELAWREFWYHIIHHFPECRDIEF
                     QQKRRGLSWDNNQKHLEAWKEGRTGYPIVDAGMRQLKEEGWMHGRVRMIVASFLTKDL
                     LIDWRYGDKHFYDYLVDYDENVDIGNWQWSASVGADPKPLRIFNPILQSQRFDPECIY
                     IKKYIPELKDEEVIKIHDPVKYKLNYYEPIVDHYEATHNTKDVYNKSI"
     gene            complement(3235..3744)
                     /locus_tag="AC159U_12315"
     CDS             complement(3235..3744)
                     /locus_tag="AC159U_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337880.1"
                     /translation="MKLHTDFTINGKHYPKGSEYNGKFIYAFFLFHMAMFGGSGFLMA
                     YNSNVELSFLYMHGGFAILIYTIFYFAIFGFDTVKWMFINAGLGLFGIYAEINWLLSL
                     FGKQASDFPRKVHVIPFLYYVLYTFLIRQAVLDFVGVRDDPERTKKTNIWYVIVSLVI
                     YSIIYIIQK"
     gene            complement(3760..4299)
                     /locus_tag="AC159U_12320"
     CDS             complement(3760..4299)
                     /locus_tag="AC159U_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337881.1"
                     /translation="MTYNNIKFDDSTPAELFVEMFWVFALISGFFIILGIGIIWSSRV
                     ELYEKILSEGVKGRELEIDFDSASKGRAGRPQYSSSYYNIQFKFKDNSGREYMDYASM
                     SVIKYQELAASFDHVHRDPGDDVRLLYLPDDPHKNWLYPYEEADYPQYYGGWGLIILS
                     IGFVIVGAIARYILKSRNM"
     gene            complement(4318..5439)
                     /locus_tag="AC159U_12325"
     CDS             complement(4318..5439)
                     /locus_tag="AC159U_12325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3337882.1"
                     /translation="MSKELIFGIIGGGPAGLATAICLAEKNYKVTLFEMNSFPADKVC
                     GEGIMPTGVKFLERHGVLGLINKSDRNEFYGVRYIPNNSKQIEGRFKPGHGLGIRRTV
                     LSEALYKKAKQYENVIIKENSELIDIYENDSLVGIEIKEGENKKKHEFDYLIGCDGIR
                     SKVRKLCDLDSNKYIENQRIGARMHFEIEPWSDLVDVYWKDYIEAYVTPVGNKLVQLA
                     FLWDNKFVRPQAGARMDERLKELFPELFQRVEGCNQVSKLKTVGPISAASKSVYKNRV
                     LLLGDAYVYLDGITGEGISIAFKEAECLAASLDSFSEAFFESYEKEVKRITSKYLSMT
                     KLALLLSYYPILRRITFPFISNKFFSRALEINMGREKWI"
     gene            complement(5485..5907)
                     /locus_tag="AC159U_12330"
     CDS             complement(5485..5907)
                     /locus_tag="AC159U_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337883.1"
                     /translation="MIRGIFLLAISLFALSLQASACEISGYNQGISSNFTYKNKQYKS
                     MLPLQKIVSSSDFNIESSKFPKDISEIINIGKTVVKKVDSELNWEVEGLGLYKYNYSN
                     CKYWYYQINYRANNQYIYINIGLNGETPELYRIDETPI"
     gene            complement(5995..>6465)
                     /locus_tag="AC159U_12335"
     CDS             complement(5995..>6465)
                     /locus_tag="AC159U_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR3337884.1"
                     /translation="IIDDVRNFLFGEPGHGGFDLASLNIQRGRDHGLPSYNDMREAVG
                     LQRVDSFSEITTDKDIQRRLSESYVTVDDIDLWIGGLAEDTYRNSQLGRLFTKLIVLQ
                     FKSLRDGDRFWFERTLSEEEIREIKNTKLSDIIIRNTDIKKGEIQKNVFKIKNR"
BASE COUNT         1981 a         1252 c          930 g         2302 t
ORIGIN      
        1 cttgcaaccg gcacatctga tgtttctatc aatttcatag atcataattt agcttcaaaa
       61 gctgttgggc ttgatccaag cattaatatg ctattagaat ctaaaaagaa gttaaagaac
      121 aaacaactaa gcaataaaat atcgattgtc agaggaatag cagaagatct accatttacc
      181 gaaggtttat ttgatgctgt tacaatatct tttggtataa gaaacactgt tgacccacag
      241 aaatcgttaa atgaaatgta cagggtacta aataaaacag gcagtatagg aattctggag
      301 ttttcacttc ctaaaaacaa gatttttggt cctgtataca tggcttatct caataacttt
      361 ttcccactaa taggttctat atttggtgca agtgaagaat acaggtactt aggtgactcg
      421 atttctaaat ttcctaatcg agacaatttt ataaacctta tgaaaaatgc aggatttaaa
      481 aactgtcgat atgaagagct taatatcggt accgtgatta tgtatagagg gtttaagtaa
      541 cttacacaga atttaatttg gttataataa gattgtataa aactgacagc tcatcggctg
      601 tagattttac atttgcagat aatatctttt caaatatcaa agatttctca attcctaact
      661 cctcacatat tagtctggaa tgataataag taggaagcca tataaataat tttttaacag
      721 tatcaatgct cgagaatatt ttaataaaat gttctatatt gagtacatag taaattctgt
      781 tattaaatgg tgatttctta agtatttttt tctcagggtc ccagatataa tgaccaaccg
      841 ttggcctcca cttaagcccg gcgtctttta gtatcaaagc cagttcgcag tgccctctat
      901 taaaaggtat agaagtcacc ttttggttgt tcttatatat tatttcagtt tctttatgag
      961 ctaaattgtc cggagttgcc gcgaccatgt atttttttat taatatttat tctattatta
     1021 tcttaagtag ttgcctatct gtatatattg tcaaattatt ctattgttaa tagtttagtt
     1081 aaatcatacg aattttttta aaaatattag agacattgat ataacaaagg ccggaatctc
     1141 accggccctt gtcttgcatt actttggtat gactactgta tctattacat gaataacgcc
     1201 atttgatgcc ataacatcgg ttccaacaac tgtagcatta tctaccatca cttttccatc
     1261 tttaacatct acagcaagtg atttaccatt cactgtttgt gcttctttga tacccactac
     1321 ttgatctgaa gtcacttttc ctgctaccac atggtaagta agaatagcta caagcttttc
     1381 tttattctca ggtttcagca gatcctccag tgtgccctct ggaagctttg caaatgcttc
     1441 atctgttggg gcaaacactg taaaagggcc ttcacccttt agtgtatcta ccaagtcagc
     1501 tgcttgtaag gctgcaacaa gggttttaaa agaccctgct gataccgctg tatcaacaat
     1561 atcttttttc tcatatttac ccgccaacgc attcatacct aaaagtgaca ttgtcactat
     1621 caatactgtg agaactttta aattgttcat tttactacct cctgtctatg ttatatctaa
     1681 ttaatgaccc atagtattag gttgtcaaac aaatttagta actgtctgta tatattgaac
     1741 tattgataat taaaatagta agcaatctag ggaagttaag agattattga aaaatattat
     1801 tatttatttg tcggaattta ttagacatat attagacata ctaaatcgat ttattataaa
     1861 catccttggt attatgagtt gcttcataat ggtcaacaat tggctcataa taattaagtt
     1921 tatatttgac cggatcatgg attttaataa cttcttcatc tttaagctca ggaatatatt
     1981 ttttgatata aatacattcg ggatcaaatc tctgggattg caaaatagga ttaaatatcc
     2041 taagaggttt agggtcagca cctactgatg cagaccactg ccaattacca atatctacat
     2101 tttcatcata atcaaccaaa tagtcataaa agtgtttgtc cccatatcgc cagtctataa
     2161 gtaaatcctt tgtaagaaat gatgctacta tcatccttac cctgccatgc atccatcctt
     2221 cttcttttaa ctgtctcatt cctgcatcga ctatcggata gccggttctg ccctctttcc
     2281 atgcctccag gtgtttttga ttattatccc aactaagacc ccttctcttc tgctgaaatt
     2341 caatatctct gcactctggg aaatgatgaa tgatgtgata ccagaattct cgccaggcaa
     2401 gttccgatat atatgtatca tctggacagc cgagagtact tacatagtta taaagctgtc
     2461 ttattgatat aatcccaaat cttatatagg gtgacagttt tgatgaaccg tctatatatg
     2521 gaaagtttct tctatttaca taatcacaga aatcaaattc ctttgccctt tgggaaataa
     2581 aactaattgg ccagttttta ttggagcaat attttaaatc ttttatagtt tctgaaatga
     2641 tttttggttt aattttagga gaatttattt tcttaatatt cagaggctta cacatctcaa
     2701 cttctttctg ccacagtcta tagaatggag taaatacttt tcttacaggg actttctcta
     2761 taggtacaat aaggctgtca ctgtattttc ttacctcgac atttaactgc tcacatttct
     2821 tctctacttc tttttcctgt tctatcgatc ccaggctgtg ggctttattg aaataaagag
     2881 cggagacatt gtgtttcttt atgatctttg taagctcttt tcttgtatcc cctcttgcta
     2941 tgtgcagata agatcctatt ttctttaact gcttattaag attttccaga gcatctacta
     3001 aaaatccaaa cctctggtta tcttgtttta ttgtcttaag aatcttatct tcaaatacaa
     3061 agacaggaat tacttcatca ctttctttaa ctgcatggta tagaccaata ttgtctttta
     3121 tacgaaggtc tcttctaaac cagaatattg ctctattggg catgtgtgaa tattttatta
     3181 ctaattcgat ggattgggat aagcctatcc cagtttcatt aaatttctac tatattattt
     3241 ctgtataata tatataatcg aataaatcac taaagaaaca atcacatacc atatatttgt
     3301 tttcttggtt ctttcaggat catctcttac cccaacaaaa tccaaaacag cttgtcttat
     3361 taaaaatgta tagagtacat aatataagaa ggggatgaca tgtaccttcc gtgggaaatc
     3421 actggcttgt ttgccgaata aagacagtaa ccaattaatt tcagcgtata taccaaacag
     3481 ccccaatccg gcatttataa acatccattt gactgtatca aacccgaata ttgcaaaata
     3541 aaatattgta taaattaata ttgcaaaacc gccatgcata tacagaaatg agagctcaac
     3601 attactgttg tatgccataa gaaatcctga tccgccgaac atggccatat ggaatagaaa
     3661 gaatgcatat ataaacttcc cattatattc acttcctttg ggataatgtt tgccgtttat
     3721 agtgaaatca gtatgtagtt tcatttgtta ttagctaact tacatatttc tacttttaag
     3781 tatgtatctt gcaatcgcac caactatcac aaaacctata cttaatatta tcaatcccca
     3841 tccaccataa tactgaggat aatccgcctc ttcgtacgga tataaccagt ttttatgagg
     3901 gtcatcaggg agatagagta gtcttacatc atcaccagga tctctatgca catgatcgaa
     3961 agatgcagct aattcctggt attttattac cgacatgctt gcataatcca tatattccct
     4021 gccgctatta tctttgaatt taaattgaat attataataa gaagaactgt attgcggtct
     4081 tcccgcccta cccttactag ctgaatcgaa atctatttct aattctctac cttttactcc
     4141 ttcggacaaa atcttttcat acaattcaac tctcgaactc cagattattc ctattccaag
     4201 aattataaaa aaacctgaaa ttaacgcaaa gacccaaaac atttccacga aaagttctgc
     4261 tggggttgaa tcatcaaatt tgatgttatt gtatgtcata aattcagcgc aatttactta
     4321 aatccatttc tctcttccca tattaatctc aagtgcacgt gaaaagaatt tgttcgatat
     4381 aaaaggaaat gtgattcttc tgagaatagg ataataagag agtagaagtg ccagttttgt
     4441 catactcaaa tacttacttg taatacgttt tacttctttt tcatagcttt caaagaaagc
     4501 ctcactaaaa ctatctaatg atgcagccag gcactcagcc tctttaaatg caattgatat
     4561 tccctctcca gttataccgt ccagatagac ataggcgtct ccaagcagta acactctgtt
     4621 tttataaaca cttttggatg ctgcagatat cggccccact gtttttagtt ttgaaacctg
     4681 attgcagccc tctaccctct gaaacagttc agggaataat tctttgagcc tttcatccat
     4741 ccttgcacca gcctgcggcc tgacaaactt gttatcccat aagaaggcta actgtactaa
     4801 tttatttcct actggtgtaa catatgcttc tatataatct ttccagtata cgtcaacaag
     4861 atcagaccag ggctcaatct caaaatgcat cctggctccg attctttgat tttcaatata
     4921 cttattacta tcaagatcgc ataatttcct aaccttagac cttataccgt cacaaccaat
     4981 taaataatca aattcatgct tctttttatt ttccccctct tttatttcta tcccaactag
     5041 actatcattc tcatatatat cgattagttc actattttct tttattatta cattttcata
     5101 ttgctttgct ttcttatata gagcctcact aagtactgtc cttcttattc caagcccatg
     5161 tccgggttta aatcttccct caatttgttt tgaattattc ggaatgtatc tgactccgta
     5221 gaattcatta cgatcggatt tgtttattag accaagaact ccatgccttt ccagaaattt
     5281 aacaccggtg ggcataatcc cctctccgca tactttatca gcagggaatg agttcatttc
     5341 aaagagggtt actttataat ttttttcagc aagacaaatt gcagttgcaa gcccggcagg
     5401 acctccacct ataattccaa atattaattc tttactcaca aaagaatgtt acataaataa
     5461 ttacatattt atataattta tttactagat aggtgtttcg tcaattctgt ataattcagg
     5521 tgtttcaccg tttaatccta tatttatata aatatattgg ttattagccc tgtagttaat
     5581 ctgataatac caatatttac agttgctgta gttatattta taaagtccga ggccttcgac
     5641 ttcccagttt agttcactat caaccttctt aacaacggtt ttaccaatat taattatttc
     5701 tgaaatatct tttggaaatt tagatgattc aatattaaaa tcagagctgc tgacaatttt
     5761 ttgtagtgga agcatggatt tatattgttt atttttatat gtaaagtttg atgaaattcc
     5821 ctggttatag cctgatattt cacaagccga tgcctgcaaa cttaacgcaa ataatgaaat
     5881 cgccagcaag aatattcctc taatcatatc tgtctcccat aatatatatt tactataata
     5941 atacgatata ttattaaaaa tgttttatta tttaacagtt tgaataacgg gcgattatct
     6001 gttttttatc ttaaacacat ttttttggat ttctcctttc tttatatctg tgtttctaat
     6061 gattatatca gagagtttgg tattttttat ttctcttatt tcctcttcac taagtgttct
     6121 ttcaaaccag aacctgtctc catctcttaa agacttaaac tgcaatacaa taagtttagt
     6181 aaataatctg cctaactgag aatttctgta agtatcctct gcaagacccc cgatccacag
     6241 gtctatatca tcaacagtaa cgtatgattc gcttaaacgc ctttgtatat ctttgtcagt
     6301 tgttatttcc gaaaaagaat ccactctttg tagtccgaca gcctctctca tatcattata
     6361 cgagggaagt ccatgatccc ttcccctttg tatgtttaat gaggctaaat caaatcctcc
     6421 atgtccaggc tctccaaaaa gaaaatttct tacatcatct atgat
//
LOCUS       DBNGSV010000347        10710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1378394, whole genome shotgun sequence.
ACCESSION   DBNGSV010000347 DBNGSV010000000
VERSION     DBNGSV010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1378394"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1566)
                     /locus_tag="AC159U_12340"
     CDS             complement(160..1566)
                     /locus_tag="AC159U_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3337885.1"
                     /translation="MKCERVEELLIDYLAGEIEPQENKIIQAHLQQCHNCSTKYEEFK
                     QIQSAFRSETLPEPSKDLLQNLSAKARQDLRHEHVPFWRKWFYSPILIPTLTTAIALM
                     IWINVGGDNTSEFGDFDDQPEYYSREVMAEKIPQQEIAGKAIVKGQGNFRAKDDLAGR
                     KQNAPASASNIAEGERRRDAAAPSLRGETPERQERSFKKVPSSIAGKNEPSAEQQKSA
                     EQARQKILDELENKKTVEYENQAIAKDSMNGVYNEQSKPQSPAAPADPNKKDDDGFSF
                     SLGSSKVMAKKVYRPSGDVELDSVGRDNKELADQDGHDYATSAKVSEQVRSAEKTEKL
                     KSIEEEDSRLIAVDTPKELADKDTLKQLGVALKQQRAGDCESAIATNEYNLKNSPGAP
                     ASVKEQTYLSLAQCYEQQNMFVKAIENYNYLQQVSPSQKPFATQKIEELNFKVQNMNV
                     PAVTEPSTTSETGVEVTK"
     gene            complement(1569..2204)
                     /locus_tag="AC159U_12345"
     CDS             complement(1569..2204)
                     /locus_tag="AC159U_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3337886.1"
                     /translation="MFGFAKKKELTDEQLMENFQNGDSAAFDQLLSKYGSKILYFIMK
                     KLNIPKPAAEDLMQEIFIKVIEKRDTFNTERKFSTWLYTVANNRCIDYMRTDKNKQTS
                     SLDKEITAEAGGATHLELVKSDSGDPEQYSYNKQIKEYVDEGVQELKEELKEVFMLRE
                     IEGLSLKEISDITGAPLNTVKTRLRTAYSNLREYLVSAGYFSQKQNLGSVK"
     gene            2397..3146
                     /locus_tag="AC159U_12350"
     CDS             2397..3146
                     /locus_tag="AC159U_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337887.1"
                     /translation="MRTVDVTNAYNLRRKYFSSEYTLTNKHTRGIITRSILNSDLSAR
                     TYEILAAYSTRFDDIKLIKLTKPQEKLNEINEWLLNVGENTGWISEIKTPSRLHSLKN
                     ELESLKSSELEPMELFGKFYPAYINIMLIALEDALIRIYIVEDTKSLIVQLHSMLNNL
                     CNLNPVGGLEPNFKALVDIYYNSLDTEEKNPHVQFIDAYAHTSFIWSQCAQFVTDYID
                     GLETGQAFSLESANKTLQQRMQKIASGYKTF"
     gene            3236..4348
                     /gene="ald"
                     /locus_tag="AC159U_12355"
     CDS             3236..4348
                     /gene="ald"
                     /locus_tag="AC159U_12355"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3337888.1"
                     /translation="MIVGILKEIKAEENRVSMTPGGVELLTGHGHTVLIEKNAGLGSG
                     FDNARYKEHGAKIVNTAIEIYKRADMVMHVKEPLPSEYKLIRKDQIVFTYLHLAASKE
                     LTSALMKTGSVDIAYETIQKDDGSLPLLTPMSEVAGRMSVLQGSKYLEMEHGGHGVLL
                     GGVPGVDPGTVLIIGGGTVGTHAAKTACGLGAKVYVLDLNLERLRYLSDIMPKNCFML
                     MSKPATLRRLIGIANVVVGAVLIPGAKAPKLVTRKMLKLMKKGAVLVDVAIDQGGCFE
                     TSKPTTHSNPIYNVDGIVHYCVANMPGALPMTSTLALTNATLPYALEIANKGWEKAMQ
                     ENNEIKLGANIINGKITYKGVADAFGLKHTPIDELI"
     gene            4360..4629
                     /locus_tag="AC159U_12360"
     CDS             4360..4629
                     /locus_tag="AC159U_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337889.1"
                     /translation="MSENTDLDEILDLIERRNKQNTEQYFIDLQSNEKAILSSASNIF
                     AAYIASGIVASGDEEKFIQKSVKEAIKIALRVEKLVSDAEEEHNI"
     gene            4834..5730
                     /locus_tag="AC159U_12365"
     CDS             4834..5730
                     /locus_tag="AC159U_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337890.1"
                     /translation="MPSSRKKDKYDKWRKEIEEERRQKIAGEAPEPEDALELENTRGT
                     NEPQEPIGVYNPKPQRRIREPSFGEELSSVFSYPFYGEGRSLMIAGTVFISIIYFLNT
                     SFGLFSLLFTFLKFFLVFYLSAFMFKIIVYSSSGEDELPDWPDIVDFFGDLLQPFFYL
                     ISTIVFSFLPTVIYYYFYREISYTNPIFLLTVIWGVFYLPMGLIAVAMFGTLSVLSPT
                     VILPSIKRITSDYFTACASLFIIIAVEIAGEHLIKKIDFPILTIPVYSFLTLYFLAVE
                     MRILGLVYYTNRHRLGWYTEDF"
     gene            complement(5784..6050)
                     /locus_tag="AC159U_12370"
     CDS             complement(5784..6050)
                     /locus_tag="AC159U_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337891.1"
                     /translation="MKFLWQHLTNLFSYLFMSSAFAGCICSFYLVIFYLKDMFGETTP
                     TEAKIAKQLFIILLFCIVIFPVSFYISHSAEKKSAEDDKYDYTK"
     gene            complement(6122..6691)
                     /gene="frr"
                     /locus_tag="AC159U_12375"
     CDS             complement(6122..6691)
                     /gene="frr"
                     /locus_tag="AC159U_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3337892.1"
                     /translation="MAELQIENDIEESILKMDETIDAFKKELSRMRTGRASASIVEHI
                     KADYYGTPTPLSQIANISVPEPRVIVIQPWDTGATSEIEKAIMQSDLGITPSSDGKLI
                     RIILPVLTEERRKELVKYVHKLSEDYRVSIRNSRKDINNKIKNSEKENEITVDDVKTF
                     MNQIQKVTDEHIAKIAELLQEKEKEILEI"
     gene            complement(6696..7412)
                     /gene="pyrH"
                     /locus_tag="AC159U_12380"
     CDS             complement(6696..7412)
                     /gene="pyrH"
                     /locus_tag="AC159U_12380"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3337893.1"
                     /translation="MPQPKYQKILLKLSGEALQGKEKFGICVDTLKAISKEIVEIQNL
                     GVQIGIVIGGGNIFRGVAGTATGMDRSAADYMGMLATVINSLALQDALESEGIVTRVQ
                     TALEIHQVAEPYIKRRAIRHLEKGRVVIFAAGTGNPFFTTDTAATLRAREIGADIILK
                     ATKVDGIYDKDPMVHKDAKKFKQLTYMDVLRKDLKVMDATAISLCREGKIPIIVFNMF
                     ETGNIYRIIMGEEIGSVVKE"
     gene            complement(7447..8043)
                     /gene="tsf"
                     /locus_tag="AC159U_12385"
     CDS             complement(7447..8043)
                     /gene="tsf"
                     /locus_tag="AC159U_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3337894.1"
                     /translation="MAEISAQMVKEIRDATGAGFVDCKNALTETDGNAEKAIEILRIK
                     GLAKASKKTGRATPEGTVTSYIHAGGKIGVLLEVNCETDFVARNDEFQEFTREVAMQI
                     AAANPTYVSEEDVPEEVLNKEREIMKAQVIEEGKPEKVADKIVDGKISKYLQENCLVN
                     QIYIRDSKKKISDLLSDLIAKVGENIQIRRFQRYQLGE"
     gene            complement(8258..8947)
                     /gene="rpsB"
                     /locus_tag="AC159U_12390"
                     /pseudo
     CDS             complement(8258..8947)
                     /gene="rpsB"
                     /locus_tag="AC159U_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            9683..10492
                     /locus_tag="AC159U_12395"
     CDS             9683..10492
                     /locus_tag="AC159U_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3337895.1"
                     /translation="MKIRVGYELIYDCPQPTPMILTLHIHYTRASDIIVTDHLITKPS
                     IPITAYRDSFGNWCSRIVAPKGEISLSADAVVNDTGKPDVVAASAHQNPVQALPEETL
                     IFLLGSRYCETDLLSEVAWDLFDKSPTGWERVLAICNFVHQHIAFGYEHARATKTAWE
                     AFNEQTGVCRDYTHLAITLCRCMNIPARYCTGYLSEIGVPPPYGPGDFASWFEAYLGS
                     NWYTFDARFNVPRIGRVLIARGRDAADVAISNTFGPTTLKSFKVWADEVSK"
BASE COUNT         2929 a         2349 c         2002 g         3430 t
ORIGIN      
        1 gagtgaaatg ggcttacaga aaccatagag gactggctaa acacaagaga attaattact
       61 tcaaatggta caccttttta aagcaaatct gactcaaatt agcacacatt ttttgcaatt
      121 atgcccagaa tgacagaaaa tgtggacgtc tagtattcct tatttcgtaa cctcaactcc
      181 agtctctgaa gtcgtagaag gctcagttac agcaggtaca ttcatgtttt gaaccttaaa
      241 gttaagctct tcaatctttt gcgttgcaaa cggtttttga gaaggcgaga cctgctgcag
      301 gtagttatag ttctctatgg cctttacaaa catattctgc tgctcatagc actgggcaag
      361 cgaaaggtag gtctgctctt ttacacttgc aggcgctccc ggactgttct ttagattata
      421 ctcatttgtt gcaattgcag attcacagtc acccgctctt tgctgtttta aggcaacacc
      481 aagctgcttt agcgtatctt tatcagcgag ctcttttggt gtatcaactg ctattagcct
      541 cgaatcttcc tcttcaattg attttaattt ttcagtcttc tcggccgacc gaacctgttc
      601 tgaaactttg gcacttgttg cataatcatg tccatcctga tctgcaagtt ctttattatc
      661 ccgtccaaca gagtctagtt ccacatctcc agatggcctg tacactttct ttgccattac
      721 cttactgctt cctagactaa aactgaaccc gtcgtcatcc tttttatttg gatcggcagg
      781 agccgcggga gattgtggtt ttgattgttc attataaaca ccattcatac tgtctttggc
      841 aatagcctga ttctcatact ccactgtttt tttattttct aattcatcca atattttctg
      901 tcttgcttgc tcagctgatt tttgttgctc tgcacttggt tcatttttac ctgcaatact
      961 tgaaggcact tttttaaatg atctctcctg tctttctggt gtttcacccc ttaatgacgg
     1021 agccgccgca tctcttcttc tctcaccctc tgctatatta gatgcagatg ctggagcatt
     1081 ttgtttcctg cctgcaagat cgtcttttgc cctgaaatta ccctggcctt ttacgatggc
     1141 ttttccggca atttcttgct gaggaatctt ttcagccatt acttctcttg aatagtactc
     1201 tggctgatca tcaaaatctc caaattccga ggtattatcc cctcccacat ttatccagat
     1261 cataagcgct attgcagttg taagggttgg aataagtatc ggagaataga accattttct
     1321 ccaaaatggg acatgttcat gccttaaatc ctgcctggct tttgcgctaa gattttgcaa
     1381 taaatccttt gatggctcgg gcagagtctc gctcctaaaa gcgctctgta tctgcttaaa
     1441 ctcttcatac ttggtagaac agttatgaca ttgctgcaga tgagcctgta ttatcttgtt
     1501 ttcctgaggc tctatctcgc ctgccagata atctataaga agctcttcta ccctttcaca
     1561 cttcatcgct atttaacact ccccagattt tgtttctgtg aaaaatatcc tgcgctaact
     1621 aaatattccc tcagatttga atacgccgtc ctaagcctcg tttttacagt atttagcggc
     1681 gcccctgtaa tatccgatat ttcttttaat gacagtcctt ctatttccct tagcataaac
     1741 acctctttca attcttcttt gagctcctgc acgccttcat ccacatactc ttttatctgt
     1801 ttattataac tatattgttc aggatcgccc gaatcacttt ttacaagctc caagtgagtg
     1861 gcgccgcctg cttcagccgt tatttcttta tcaagtgaac ttgtctgttt atttttatcc
     1921 gttctcatat aatcaatgca cctgttgttt gcaaccgtat aaagccatgt tgaaaatttt
     1981 ctctctgtat taaatgtatc cctcttttca attaccttta tgaatatctc ctgcatcagg
     2041 tcctctgctg cgggttttgg tatattgagc tttttcatga tgaagtacaa aatctttgag
     2101 ccatacttgc tcagaagctg atcgaacgca gcactatcgc cattttgaaa gttttccatc
     2161 aattgctcat ctgtaagctc ttttttcttt gcaaaaccaa acacagccgc gaccttgttt
     2221 ataagtttaa ttaattatac gacatttatt ctaaaaagtt ttgggaattg ataaaataat
     2281 tatgattaag aaagatgaat acaccattgt gcttaaatcc aaaaaattat ataatgataa
     2341 caagaccgga agtctaataa ttcccacgcc tatctgtttt ttatataaat agtaagatga
     2401 gaaccgtaga cgtaacaaat gcttacaacc tgagaagaaa gtactttagc tccgagtaca
     2461 cgcttacaaa caaacacaca cggggaataa ttacaagatc aatcctaaac tcagacctaa
     2521 gcgctcgcac ctatgagata cttgcagcat attctaccag gtttgatgat ataaaactta
     2581 taaaacttac aaagcctcaa gagaagctaa atgaaataaa tgaatggctg ttaaatgttg
     2641 gcgaaaatac agggtggata tcggagataa aaacccccag cagacttcac agtcttaaaa
     2701 atgagctcga atcattaaaa tcttccgaat tagagcccat ggagcttttt ggaaagtttt
     2761 atccggcata catcaatatt atgctcatag ccctagaaga tgcgcttata cgaatttata
     2821 tagtagaaga cacaaagtca ctgatagtgc aacttcactc catgttaaat aatctttgca
     2881 atttaaatcc cgtgggtgga ctggagccta acttcaaagc ccttgtagac atttactata
     2941 atagtctgga taccgaagag aaaaatccgc atgtgcagtt tatagacgcc tatgcccaca
     3001 catcatttat atggtctcaa tgtgctcagt ttgtcactga ctatatagac ggacttgaaa
     3061 caggacaggc cttctcgctt gaaagcgcca ataaaaccct gcagcaaaga atgcagaaga
     3121 ttgcaagcgg gtataagacc ttctagctca aataatattg ttttacttta ccggttattt
     3181 gaatcagtct taatataatt tactcttata tatatcactt attgcaggag aacacatgat
     3241 agtaggaata ctaaaagaga taaaggctga agagaacagg gtttcaatga cccccggggg
     3301 agtggaactt ttaacagggc acggccacac ggtgcttata gaaaaaaatg cggggcttgg
     3361 aagcggcttt gataatgctc ggtacaaaga gcatggtgct aagattgtaa acacggcaat
     3421 agagatttat aaacgagcag acatggtgat gcatgtaaaa gagcctctgc catctgagta
     3481 caaacttata agaaaagacc agattgtttt tacttacctt catcttgcag cttcaaaaga
     3541 actgacttct gcactaatga aaactggctc ggtagatata gcttatgaaa ctatacaaaa
     3601 agatgacggg tctttgcctc ttttaacccc catgagcgag gttgctggca ggatgtcagt
     3661 gcttcaggga tcaaaatacc tggagatgga gcacggcggg cacggagtgc ttcttggggg
     3721 agtgccaggt gttgaccccg gtactgttct tatcattggt ggaggcacag tgggtactca
     3781 cgccgcaaag acagcttgcg gccttggagc aaaggtttat gtactggatt taaacctgga
     3841 gcgtctcaga tacttaagtg acatcatgcc taagaactgt ttcatgctaa tgtccaaacc
     3901 cgctacactt agaagactta ttggaatagc gaatgtagtt gttggcgctg tactaatccc
     3961 cggagccaag gcccccaaac ttgttacccg taaaatgtta aagcttatga aaaagggagc
     4021 cgtgcttgta gacgttgcaa ttgatcaggg aggatgtttt gaaacatcaa aacccactac
     4081 tcactccaat ccgatctata acgtagacgg cattgtgcac tattgtgtag ccaatatgcc
     4141 gggggcactg cccatgacat ctactctggc tcttacaaat gcaactcttc cctatgcgct
     4201 tgaaattgca aataaaggct gggaaaaagc gatgcaagaa aataatgaaa taaaacttgg
     4261 tgcaaatatt ataaatggta aaattacata taaaggtgta gctgatgctt ttggtctcaa
     4321 acacactcca attgatgagt taatttaaca atacagctta tgagcgaaaa tacagacctt
     4381 gatgagattc tcgatcttat agaaaggcgc aacaaacaaa atacagagca gtattttatt
     4441 gacctgcaga gcaatgaaaa agctatattg tcatcggctt caaacatatt cgctgcatat
     4501 atagcatctg gcattgtcgc ttcaggggat gaagagaagt ttattcaaaa atcggtaaaa
     4561 gaggctatta aaattgcact aagagtagaa aaattagttt ctgatgctga agaagagcat
     4621 aatatctgac aaatacaatt tatatatttc cagccaccac caaggcattc catgcgcctt
     4681 gcccctatat atatgctatc agatttcatc aacaccccct gtccacaacg ccatatcaat
     4741 attgacaatc cagcaaccat gataacatat gagatatgcc ccctgtaata tgcagtctgc
     4801 taacaggcag caaattcacc aaaggagatc acattgccca gttccaggaa aaaagataaa
     4861 tatgataaat ggagaaaaga gattgaagaa gagcgcaggc aaaaaattgc tggtgaggct
     4921 ccggagccag aagacgctct tgaactagaa aatactcgag ggactaatga acctcaagag
     4981 cccataggag tctacaatcc aaaacctcaa aggcgcataa gagagccatc atttggtgaa
     5041 gaattatcta gcgtattctc ttatccattc tacggagaag gaagatcgct tatgattgca
     5101 ggcactgtat ttatttctat tatatatttc ctgaatacat catttggatt atttagtttg
     5161 ttattcactt ttttaaagtt tttcctggtt ttttatcttt cagcttttat gttcaagatc
     5221 atagtgtatt catcaagcgg tgaggatgag cttcctgatt ggccggatat cgtagatttt
     5281 tttggagacc tcctgcagcc tttcttctat ctgatatcca ccatagtttt tagttttttg
     5341 cctacggtaa tatattatta tttttacaga gaaatttcgt atactaatcc tatcttttta
     5401 ttaaccgtga tctggggagt gttttacctt ccaatgggcc tcattgcggt tgctatgttt
     5461 ggtacactat ctgtcctgag ccccacggtt attcttccat caataaaaag aattacttct
     5521 gattatttca ctgcatgcgc atccctcttt attataatcg ctgttgagat tgctggagag
     5581 catttaatca agaaaataga ctttcctata ttaaccatac ccgtatacag cttccttacg
     5641 ctttattttc ttgcagtgga aatgagaata ctggggctgg tttactatac aaacagacac
     5701 cgactaggtt ggtacactga agacttttga ttctaataaa tgttattgag actcaaacga
     5761 agaatgaagt tatttaaaaa actttacttc gtgtaatcat acttatcatc ctcagcgctt
     5821 ttcttttcgg cagaatgaga gatgtaaaaa ctcacaggga agatcactat acaaaatagc
     5881 agtataataa aaagctgttt tgcaattttt gcctctgtag gagtagtctc tccaaacata
     5941 tcctttagat aaaaaatcac aagatagaac gagcatatgc acccggcaaa ggccgatgac
     6001 ataaaaagat aagagaaaag attagttaga tgctgccata aaaatttcat ataggattat
     6061 tttaattgaa atatttaaag atgtatagtg ctgctacgaa atagatttag tctagctaaa
     6121 attagatttc cagaatctct ttttcttttt cctgcagcag ttcggcaatt ttggctatat
     6181 gttcgtctgt aaccttttgt atttgattca taaaggtttt tacatcatct acggttatct
     6241 cgttttcttt ttcagagttc tttattttat tatttatatc ctttctggaa tttcttatag
     6301 atactctata atcttcagac agcttgtgga cgtatttcac aagctccttt ctcctctctt
     6361 ctgtcagtac aggaagaata atgcggatta atttaccatc actcgatggt gttattccaa
     6421 gatcagactg cattatggcc ttttcaattt cactcgtagc acctgtatcc cagggctgta
     6481 tgactattac ccgaggttct ggcactgaaa tatttgcaat ctggctaagc ggggtaggtg
     6541 tgccgtaata atcagccttt atgtgctcta ctatggatgc cgacgccctt ccggttctca
     6601 ttcgggatag ctctttttta aatgcatcga tggtctcgtc catcttaagg attgattctt
     6661 caatatcatt ttcaatttgt agttcagcca taatatcact cctttactac tgatcctatt
     6721 tcttcaccca taattatcct ataaatattg cctgtttcaa acatgttaaa cacaattata
     6781 ggtattttac cctccctgca aagagatatt gccgtggcgt ccattacctt taagtccttt
     6841 ctgagtacat ccatataggt tagctgcttg aatttcttgg catccttatg caccatggga
     6901 tctttatcat atattccatc caccttagtg gctttaagga ttatatcagc acctatctcc
     6961 cttgctctta gagttgccgc cgtgtcggta gtgaaaaaag gatttccggt acccgctgca
     7021 aagatcacaa ccctgccctt ttcaaggtgc cttatggctc ttctttttat gtagggttct
     7081 gcgacctggt gaatttcgag tgcggtctgt actcttgtga ctatgccctc agattcaagt
     7141 gcatcctgca gagccaggga attgattaca gttgcaagca tacccatata atcggcagca
     7201 gacctgtcca tgccggttgc tgtgcctgct acccccctga atatgttgcc gccgccaata
     7261 acaatgccaa tctgcacacc caggttttga atctctacaa tttctttgga aattgccttt
     7321 agggtatcta cgcaaatacc aaatttttct ttaccctgaa gggcttcgcc acttagcttt
     7381 aaaagaattt tttgatattt tggttgcggc atgtattaag ctttcaaaat aatatttggc
     7441 cttaagctac tctccaagct gatatctttg aaaccttctt atttgtatgt tctcgcctac
     7501 ttttgcaatc aggtcgctta gcagatcaga tatctttttc ttggaatccc taatatatat
     7561 ctgatttacc agacagtttt cttgcagata ctttgatatt ttaccatcaa caattttatc
     7621 cgctactttt tcgggtttcc cttcctctat cacctgagcc ttcatgatct ctctttcctt
     7681 gtttaatacc tcttcaggca catcttcttc ggaaacataa gtagggtttg ctgcagcaat
     7741 ctgcatagca acttcccttg taaattcctg aaactcatcg tttcttgcaa caaaatccgt
     7801 ctcgcagttt acttcgagaa gtactccgat cttacctccg gcatgtatgt atgatgttac
     7861 cgtaccttcc ggggttgctc ttcctgtttt ctttgatgcc tttgcaaggc cctttattct
     7921 tagaatttca attgcttttt ctgcattacc atcagtttct gtaagtgcat ttttgcagtc
     7981 tacaaaccct gcacccgtag cgtctctaat ttcctttacc atttgtgctg aaatctcagc
     8041 catcttttga ctcctccttc tttgtttctt ctactgttgc ggctgcttct ccagccttac
     8101 tctcagatac cgctatagat tcagaagatt tggtagctcc ttctttttcc tcaccaaact
     8161 ctttaaatac aaataccttt ctttcaacca ccacaggtga ttcctcttcc ttgggtttaa
     8221 cgatctcacc ggcagctact ctctgatcat aaatgtgctt gccttcaatt gccgcatcgg
     8281 ccacttttga agcaaacaat ttaattgccc ttatggcgtc gtcgttactt ggaataatat
     8341 aatcagcatc ctgcgggtcg cagttcgtat caaccactgc aactattggg ataccaaggt
     8401 ttctggcttc cctcactgct atatactctt ttcttgtatc tataataaac atcgcatcag
     8461 gagccctctt catacccact attccattta acagatactc aagctttagt atctcccttt
     8521 ttaggctctt tgcttcattt ttgggaagaa gctccatctg cccttcttct tccatttttt
     8581 tgagttcgag caggtaatcc actctccctc ttatggtgtt aaaatttgta agtgttccgc
     8641 caagccatct ggagtttaca tagtgaagcc cggctctggt cgcttcttct tccacgatgc
     8701 cctgggcctg ctttttggtt cctaccataa ggatttcccc tccatcggca accacctgct
     8761 ttacgtatgc aaatgcgtct tttgaaagtg tgactgtctg ctgaaggtca atgatgtgaa
     8821 tgccgtttct gtttccaaaa atgtagcttt tcattcttgg attccagtgg ctaatctgat
     8881 ggccaaaatg aacaccggcc tctagaagct gtttcatgct aatgttttcg cttgcctcct
     8941 ggggcatagc tggcggtgcc gctgtggttg gcgcctcttg tgctgcaggt ggtgcttgtg
     9001 tcccgggtgc tccgggcact tgttgtactt cgtcgctcat aatatacgct ccttaaaaac
     9061 tctcctcaga atttgtttat ttataaaaat aaacccgaca taaataacat atcaggaggg
     9121 tttttcaagg gtacaaactt ttttgttaag gattatttcg tcatcctgaa cttgattcag
     9181 gatctcttat tagattctga aatgaattca gaatgacaca agggaaaata ccccaaccca
     9241 gagccaaatt ctcaaattgt catcttttta ctcgtacccc tgaaaatatg ggaatatgac
     9301 aatttgaacc agattcccca tgtaaatagt gtctgtcact aatttgtcac taatttgtcg
     9361 gttgcgatct ttgtgataat gattccaaaa tatactgctc cctattaaga acatggtaag
     9421 agattaagct agtaagtttt ctgaaattca ggaacgggag caatggctaa ccaaatataa
     9481 gattgacgat caatttgtat cgtatcagca ttttgtattt gactcataat attattcctt
     9541 gcaaaatgtc tgatattaat atgtaatctt aatgatattg tataatatta ttcaggtaat
     9601 taccatataa tcaccatgct ccatcaatga gttaacgtgc ttcttccact caatgttagg
     9661 gcactactga aggagactaa ttatgaagat acgagtcggc tacgaactaa tctacgactg
     9721 tccacagcca acacctatga tactgacgtt acacatccat tatacgcgtg cttccgatat
     9781 catcgttacc gatcatctca tcaccaaacc gtccatccca atcactgcct atcgtgattc
     9841 tttcggcaac tggtgcagca ggattgttgc accgaaaggc gagataagtc tttcagccga
     9901 tgcggtcgtg aacgatactg gtaagccgga tgtggtcgct gcctcggctc atcaaaatcc
     9961 ggtgcaagct cttcccgagg agacgctgat atttcttttg ggaagtcggt attgtgaaac
    10021 cgatctgttg tccgaggtgg cttgggacct attcgacaaa tcgcctaccg gttgggaacg
    10081 cgtcctggcc atctgcaatt tcgtccatca acatatcgcc tttggttacg agcatgcccg
    10141 cgccacgaag acagcctggg aagctttcaa tgagcaaacc ggtgtttgcc gtgattacac
    10201 gcatcttgcc atcacattat gtcgttgcat gaatattcct gcacgctact gcactggata
    10261 tttgagcgaa atcggcgtac cgccgccata cggtcctggg gatttcgctt cgtggtttga
    10321 agcatacctt ggaagtaatt ggtatacctt tgatgctcgg ttcaacgtcc cacgtattgg
    10381 ccgagtgctt attgcacgag gtcgcgatgc tgctgatgtc gcgatcagca acacgttcgg
    10441 ccccaccacg ctgaaaagtt ttaaggtctg ggcagatgag gtgagtaaat gaaataaacc
    10501 cgccataaac aacatatcag gggggggggt tcaaggggtt tgagtatata ggcactaaat
    10561 ttgtcatctc gaaccacaaa ctttattgtc aagataaagt ttggctgtat atgatcgaag
    10621 tctgtaattt gatagtttaa aaacaactgt ctgaattcaa attaggagag atctgctttt
    10681 aatcaaacgc attgagcctg gtcgaaatgc
//
LOCUS       DBNGSV010000348         7569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1310746, whole genome shotgun sequence.
ACCESSION   DBNGSV010000348 DBNGSV010000000
VERSION     DBNGSV010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1310746"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..543
                     /locus_tag="AC159U_12400"
     CDS             175..543
                     /locus_tag="AC159U_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337896.1"
                     /translation="MMNHSFPNQISTSGFTTDNPLFQLVSDSPQNNLMFFTHMNEDWT
                     NGCGNFCCICGMYELHMIAIDPRLQRSMCIGCYNMKTKLDSQIDKMLLPYRLTEDIFV
                     EKHAKPAPVMNAIPISRPTF"
     gene            complement(524..982)
                     /locus_tag="AC159U_12405"
     CDS             complement(524..982)
                     /locus_tag="AC159U_12405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3337897.1"
                     /translation="MYELPIIQTSAWDDREIQKFLTGSLIPMRLAVNDTAGFPLICSL
                     WYLFDNNKLYCATSIKSKICRLLQNNNRCAFEIAPNHPPYKGVRGQAKVNIAPDSGKL
                     ILKKLMTRYLGTTDSNLAKWLLSRERSEQRLIIEPQWISAWDYSARMSAD"
     gene            1128..1388
                     /locus_tag="AC159U_12410"
     CDS             1128..1388
                     /locus_tag="AC159U_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337898.1"
                     /translation="MYRQINEIDSYEYKANKVMNALNDSVNMNIDNLIKETNIDKTEI
                     IQVIENLEKSQYIELESYDHVHISKYGRKINNGEINVGYGPI"
     gene            complement(1510..1863)
                     /gene="rplT"
                     /locus_tag="AC159U_12415"
     CDS             complement(1510..1863)
                     /gene="rplT"
                     /locus_tag="AC159U_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3337899.1"
                     /translation="MRVRKAVASRKRRKRILKQAKGFWGRRSTNIRRAKETLMRAWAF
                     AYRDRKNKKRTFRRLWIVRINAAVRAHGLSYSKFINGLKKAGVELDRKVLADLAVRDA
                     NSFSKVVDTAKASLG"
     gene            complement(1893..2096)
                     /gene="rpmI"
                     /locus_tag="AC159U_12420"
     CDS             complement(1893..2096)
                     /gene="rpmI"
                     /locus_tag="AC159U_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3337900.1"
                     /translation="MPKHKMKSNRSAAKRFKTTGSGKIRRMAGGKSHLNVKKARKRKR
                     RLLSPDIIEGTRAIKIKSYVPYV"
     gene            complement(2083..2745)
                     /gene="infC"
                     /locus_tag="AC159U_12425"
     CDS             complement(2083..2745)
                     /gene="infC"
                     /locus_tag="AC159U_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3337901.1"
                     /translation="MARTPEIRVNRRIRARELRLVDADGEQLGILSLDEALNRAEESG
                     LDLVEVAPNANPPVCRIMDYGKYKYDLKKQTGAKKQKVHTLKEIKFRPNIGQHDLEVK
                     VNRIRDFLTEGHKTKVRIFFRGREITHPDRGRELAHGILETLGELATVESNPKLEGKN
                     LVMVLSPAKKKEQQNTDNKQKDNKEVSKQAENKENNEQADNKEENKQTENKERVEKDA
                     ET"
     gene            complement(2729..4675)
                     /gene="thrS"
                     /locus_tag="AC159U_12430"
     CDS             complement(2729..4675)
                     /gene="thrS"
                     /locus_tag="AC159U_12430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3337902.1"
                     /translation="MSEIQVKIDNKDTLTLNAGVSVSEIVAESGTKSDIIGAVIDERV
                     VDLQYEVREDSEIEFIKSGSDEALFFVRHTAAHVMAEAVQKLFPGTKVTIGPVINNGF
                     YYDFDYEKAFTEADLEKIEAEMNRIINEKKPLIRKSVPRSEAVKTFDGMGENFKVEII
                     KDLPEDEDITIYNQGDWFDLCRGPHAPDTGYIKAFKLLSVAGAYWRGIETNPMLQRIY
                     GTAFLNKKDLKTYLLKLEEAKKRDHRRLGIDLDLFSIQEEIGSGLVLWHPKGSKVRRI
                     IEDLWRAEHDRFDYDLLFTPHIAKLDLWKTSGHLDFYKENMYSSMEVDNSEYQIKPMT
                     CPFHIMVYKTKVRSYRDLPLRWAEIGTVYRYERSGVLHGLLRVRGFSQDDAHIFCRPD
                     QIEQEVMSVIDLTLLFLKIFGFDDYEVFISTRPEKYVGDEQNWEHATNSLKSALEKRD
                     IKYEIDSGGGAFYGPKIDLKIKDVLGRSWQCSTVQVDFNLPERFNISFVGEDNARHTP
                     IMIHRAIFGSFERFFGILIEHYGGAFPFWLSPVQVKIATISDEHIEYGEKVYNILKEN
                     NIRVEKDFRNEKLGLKVREAQLQKVPYLLVLGSKEAEEGLISPRKLGGTNIPSMTTEQ
                     FLTHIEMENSAYTWREVEANSENT"
     gene            complement(4689..4763)
                     /locus_tag="AC159U_12435"
     tRNA            complement(4689..4763)
                     /locus_tag="AC159U_12435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4729..4731),aa:Val,seq:gac)
     gene            4936..5820
                     /locus_tag="AC159U_12440"
     CDS             4936..5820
                     /locus_tag="AC159U_12440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3337903.1"
                     /translation="MVLNTEDFITGGPKPRKGKVRDIYELGDKLLIVSTDRISAFDVI
                     IPNGIPEKGKILNQISCFWFEFTKDIVKNHLISYDVDDFPHELHKYANVLDKRSMLVQ
                     KTNPLPVECIVRGYIAGSGWKEYQQTGSTSGIDLPKGLKESEKFSEPLFTPSTKAEIG
                     LHDENISYESVVDMIGGGLAKTLKDLSISIYTKAHDYARSKGIIIADTKFEFGQDLKT
                     GELILIDEVLTPDSSRFWPLDEYEPGRPQKSFDKQFVRDYLNTLNWDKTPPAPKLPDD
                     IVEKTKSRYEEVYEILTS"
     gene            5824..6015
                     /locus_tag="AC159U_12445"
     CDS             5824..6015
                     /locus_tag="AC159U_12445"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR3337904.1"
                     /translation="MLYRDNNLSPCKSICILDESKKMCKTCFRTITEIANWSRYSKDE
                     KLDVLQKIELRMKSANSEN"
     gene            6194..7147
                     /locus_tag="AC159U_12450"
     CDS             6194..7147
                     /locus_tag="AC159U_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3337905.1"
                     /translation="MQDFLHKDLIFEDINCLKQLCGEKNSNIKEIEKLFEIKLHTRGN
                     KITLDGETKNTDSAEKFITQIYSLITKGYHLEPGDIELASRIILSDKHALEDIFLDTI
                     CVSTRKRIIAPKSLAQKHYIDAIRKNDIVFGIGPAGTGKTYLVMALAVSAFLNKQFNR
                     IILTRPAVEAGEKLGFLPGDLTQKVDPYLRPLFDALYEMMDYDKASRLIEKGAIEVAP
                     LAFMRGRTLNDAFVILDEAQNTTSMQMKMFLTRLGFNSKAVITGDVTQIDLEKNETSG
                     LIHSENILKNIPNLDFVHFSNKDVVRHPIVKKIIEAYEKEV"
     gene            7186..>7569
                     /locus_tag="AC159U_12455"
     CDS             7186..>7569
                     /locus_tag="AC159U_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337906.1"
                     /translation="MRTIYITLPVLIIAISFLAGCTGKWQNPNDTIAAQELKIRNLLI
                     MPNIYDHAGVILEGKIWDLDFVEDKENPDKVYSMFKLADKDGNFVHVTSEGMTTLLQE
                     GEIVKVIGIHRVLYGLETRTISYLVD"
BASE COUNT         2345 a         1478 c         1332 g         2414 t
ORIGIN      
        1 gattattaat attttgggga aaaattctaa aaatattaag tgctaatacc cgggatagaa
       61 attaaatcat tttgagacaa tagaacttat ttcatattat acttgaatta tgcagtgtaa
      121 tatattataa ttactacaga attagatagt aacgaaatga ttttgaggag gtggatgatg
      181 aatcatagct ttccgaatca aattagtact tctggtttca ccacggataa cccgctgttt
      241 cagctggttt cagactcacc acagaacaat cttatgtttt tcactcatat gaatgaggac
      301 tggacaaatg ggtgcggcaa cttctgttgt atttgcggca tgtatgagct tcatatgatt
      361 gccattgatc ccagacttca gaggtcgatg tgtataggtt gttacaatat gaagaccaag
      421 ctggacagtc aaatagacaa gatgcttctg ccctacaggc taactgaaga catctttgtg
      481 gaaaaacatg caaagcctgc tccagtgatg aatgcgattc cgattagtcg gccgacattc
      541 tagctgaata gtcccaagca gaaatccatt gaggttctat aatcagcctt tgctcactcc
      601 gttctctgct caaaagccat tttgccagat tggaatcagt tgtcccaaga tatctggtca
      661 taagtttttt gagtattaat ttgccggagt caggagctat atttactttt gcctgacccc
      721 gtacaccttt atatggtgga tggtttggtg caatttcaaa agcacagcga ttattatttt
      781 gcagcaacct acatatcttg gattttattg aggttgcgca atataatttg ttgttatcaa
      841 acaagtacca cagtgaacag attaaaggaa aaccggcagt atcattaaca gccaacctca
      901 tagggatgag agagcctgta agaaatttct gtatttctct gtcatcccag gcactagttt
      961 gaattatagg tagttcatac atgtatatac tttacctccg tattatatga taggttaaag
     1021 tgtgtctagt atttgtcaaa tattatttac atattactag acaattactt gacatcagtg
     1081 aaatatattg cataatttat ataaattcaa accaaaggag atagacaatg tatagacaaa
     1141 taaatgaaat tgattcatac gaatataaag caaacaaggt tatgaacgca ctaaacgatt
     1201 cagtaaatat gaatattgat aatcttataa aagaaacaaa tattgataaa actgaaatta
     1261 ttcaagtaat agaaaattta gagaagtctc agtatataga acttgaaagt tacgatcatg
     1321 tacatatatc aaaatacgga agaaagatta ataacggtga gattaatgtg ggttatgggc
     1381 ctatatagta tttaaattca gcaatttaac aacagggcat tccccacccc tgttgttgag
     1441 ttacaaataa tcacaaaaat actgctattc aaatatgtgt gtaatgtgat aatccaaaat
     1501 taaataaaac taccccaaac tagccttagc agtatcaact actttactaa aactattagc
     1561 atctcttacg gcaagatcgg caaggacttt tctgtcaagc tcaactccgg cttttttgag
     1621 accatttatg aatttactgt aagaaagtcc atgagccctt actgcggcat ttattcttac
     1681 gatccaaagt cttctgaagg ttcttttctt attttttctg tctctgtaag caaaagccca
     1741 ggctctcatt aatgtttctt tagcccttct tatgttcgta cttcttctgc cccaaaaacc
     1801 cttggcctgc tttagtattc tttttcttct ttttctcgat gctactgcct ttctaactct
     1861 catcttattt aactccgatt tctaatttta ttttatacat agggaacgta actttttatc
     1921 ttaattgctc tagtaccctc aataatatca ggacttagca gtctcctctt tctctttctt
     1981 gcctttttaa catttaggtg actcttacca ccggccattc ttctaatctt accagagcct
     2041 gttgtcttaa acctcttggc agcacttctg tttgatttca ttttatgttt cggcatcttt
     2101 ttctaccctt tctttatttt ctgtttgttt attttcttct ttattatctg cttgttcatt
     2161 attttcttta ttttctgcct gtttacttac ttccttatta tctttttgtt tattatctgt
     2221 attctgctgt tctttctttt tggccggaga taataccata acaaggttct taccttcaag
     2281 ttttggattg gattctactg ttgcaagctc tccaagagtc tccagtatcc catgcgcgag
     2341 ttctctaccc ctatcgggat gtgtaatttc tcttcctcta aaaaatatcc tgacttttgt
     2401 tttatgaccc tctgtcaaaa agtctcttat tctatttact tttacctcta aatcatgctg
     2461 gcctatattg ggcctgaact ttatttcttt gagtgtatga actttttgct tctttgcccc
     2521 ggtctgcttt tttaaatcat acttatattt gccataatcc attatcctgc aaacaggtgg
     2581 attggcattt ggtgcaactt ctacaagatc aagccccgat tcttccgctc tatttaatgc
     2641 ttcatcaaga gataaaatac caagctgttc accatcggca tctacaagcc taagttccct
     2701 ggcccttatt cttctgttta ccctaatctc aggtgttctc gctattagct tctacctccc
     2761 gccaagtgta tgcgctgttt tccatttcta tatgtgttaa gaattgttct gttgtcattg
     2821 agggtatatt agtacctccg agttttctgg gtgagattaa accttcctct gcttctttgc
     2881 tgccaagcac taacaagtac ggtacttttt gaagctgagc ctcccttact ttcaaaccaa
     2941 gtttctcgtt tctaaaatcc ttttcaaccc ttatattatt ttctttaagt atattatata
     3001 ctttttcacc atattcaatg tgttcgtcac taatcgtagc aatcttcacc tgtaccggac
     3061 tgagccagaa aggaaaagcc ccaccataat gctcgatcag tattccaaaa aacctctcga
     3121 aagatccaaa tatagcccgg tgtatcatta tcggagtatg acgagcattg tcttctccca
     3181 caaaactgat attaaatctt tcaggaaggt taaaatctac ctgaactgtg gaacactgcc
     3241 aagacctacc taaaacatct ttaatcttaa ggtctatctt gggaccgtaa aaagccccgc
     3301 cgccgctgtc tatttcgtat ttaatgtctc ttttttcaag cgcactttta agtgagtttg
     3361 tagcatgctc ccaattctgc tcatcaccaa catatttttc tggtcttgtg gagataaata
     3421 cttcataatc gtcaaatccg aatattttca aaaacagcag agtaagatca ataacactca
     3481 taacttcctg ttcaatctga tcaggtctgc aaaatatatg ggcatcatcc tgagaaaatc
     3541 ctcttacacg cagaagcccg tgaagcacac cagatctttc atatctgtaa acagttccaa
     3601 tctctgccca tcttaagggg aggtcacggt agcttctcac ttttgtttta taaaccatta
     3661 tatgaaacgg gcatgtcatg ggtttaatct gatattcaga gttatctact tccatggatg
     3721 aatacatatt ctctttataa aaatccagat gcccgcttgt tttccataaa tcaagctttg
     3781 caatatgtgg agtaaataac aggtcataat caaacctgtc atgctccgct ctccacaggt
     3841 cttcaataat ccgcctgact tttgagccct tggggtgcca aagaactaac cctgagccta
     3901 tctcttcctg tatgctgaat agatcaagat caattccaag ccttctgtga tctctttttt
     3961 tagcttcttc gagctttagt aagtatgtct ttaaatcttt tttgtttaga aacgctgtac
     4021 cataaattct ttgcagcata gggtttgttt ctatgcctct ccagtaagca cctgccacac
     4081 taagtagttt aaatgctttt atgtatcctg tgtccggcgc atgggggcct ctgcaaagat
     4141 caaaccaatc cccctggttg tagatggtaa tatcttcatc ttcgggaagg tctttaataa
     4201 tctctacctt gaaattttca cccattccgt caaatgtttt tacagcttcg cttcttggaa
     4261 cactttttct tattaaaggc tttttctcat taataattcg attcatctcg gcttcaatct
     4321 tttccagatc agcctcggta aaagcttttt cataatcaaa gtcataataa aaaccgttat
     4381 taattactgg acctatagta acttttgtcc ccggaaatag tttttgcaca gcctctgcca
     4441 taacgtgagc agctgtatga cgcacaaaaa ataacgcctc gtcgcttcca cttttaataa
     4501 actctatttc cgagtcttct ctaacctcgt actgaaggtc aacaaccctc tcatctataa
     4561 cagcaccgat gatgtcagac tttgttccag actccgcaac tatttcgctc actgaaactc
     4621 cggcgtttaa tgtcagcgta tctttattat ctatttttac ctgtatttca ctcataatta
     4681 ttaagcagtg gtgagcacgg caggatttga acctgcgacc tcttgcatgt caagcaagcg
     4741 ctctacccct gagctacgcg ctcaaatata ttgatgaacg gcttattcta cttatatata
     4801 ggcttgattg tcaagacagc acagctattt taattaataa atataacaag aactaaaaca
     4861 ttttttgatt taattattat tttacgtagc ttaaatacaa catttgaggg caggtgagta
     4921 aaaattagca gcaatattgt tctaaatact gaagacttta taacaggcgg accaaagcca
     4981 agaaagggaa aggtaaggga tatatatgag cttggagata aactgcttat agtttctaca
     5041 gacagaatct cggcgtttga tgtgataatt ccaaacggta tcccggaaaa aggtaaaata
     5101 ttaaatcaga tttcatgctt ctggttcgaa tttaccaagg acatagtaaa aaaccattta
     5161 atatcatatg atgtagatga cttcccgcat gaattacata aatatgcaaa cgtcctggat
     5221 aaaagaagca tgctggtaca aaagacaaac cccctgccgg tggaatgtat agtgaggggt
     5281 tatattgcag gctcagggtg gaaagagtat cagcaaacgg gatcgacgtc tggcatcgat
     5341 cttccaaagg ggttaaaaga atccgagaaa ttttctgagc cgctttttac gccttctaca
     5401 aaagctgaga ttggtcttca tgatgaaaac atatcatacg aatctgttgt ggatatgatc
     5461 ggagggggtt tagcaaagac tctaaaagat ttaagcatta gcatatatac aaaagctcat
     5521 gattatgccc gaagcaaagg aataataatt gcagatacga agtttgagtt tggacaggat
     5581 ttaaaaactg gagagctaat attaattgat gaggttttaa cccccgattc atcaagattc
     5641 tggccactgg atgagtacga acccggaaga ccacaaaaga gttttgataa acagtttgta
     5701 agggattatc taaataccct caactgggat aaaacccctc ctgccccaaa actaccggat
     5761 gatatagtgg aaaagacaaa aagcagatac gaagaagttt atgagattct aacctcttaa
     5821 tttatgttat atagagacaa taacctgtca ccatgcaaga gcatctgcat acttgatgaa
     5881 agtaaaaaga tgtgcaagac ctgctttaga acaattacag agatagcaaa ctggagtaga
     5941 tacagtaagg atgagaagtt ggatgttcta caaaagattg aacttagaat gaaatcagca
     6001 aattcagaaa attagtatag taaatacaaa caatggttgt ttgactaaat tttgttctgt
     6061 ataattatta attaacaatg gggtacaaat attaaatcaa tttatttatt cataacactt
     6121 ttctataacg gtaatctaga tagatgcaaa tctaatgcca actttatgag cctaatactc
     6181 tcaacataac tctatgcagg attttctaca caaagacctc atatttgaag atattaactg
     6241 cctaaagcag ctctgtggtg agaaaaactc caatattaaa gagattgaaa aactattcga
     6301 gataaaactt cacacccgcg gaaacaagat cacactcgat ggagaaacaa aaaatactga
     6361 tagtgcagag aagtttatta ctcagatata tagcctcatt accaagggct accatctgga
     6421 gccgggcgat atagagcttg cctcgaggat tatactttca gataaacacg ccttggaaga
     6481 catattcctg gacactattt gtgtgtccac aagaaaaagg atcattgctc caaagagcct
     6541 ggctcaaaag cactatattg atgcaatcag aaaaaacgat attgtattcg gtatcggccc
     6601 cgcgggtacc ggaaagacat atctggttat ggctcttgca gtctcggctt ttttaaataa
     6661 acaatttaac aggatcattc ttacaagacc cgctgtggag gccggagaaa aactcgggtt
     6721 tctcccagga gatctcactc aaaaagtaga cccttaccta agaccgcttt ttgatgcgct
     6781 ttatgagatg atggactatg acaaagcatc aaggcttata gaaaaagggg ccatagaagt
     6841 agcgccactg gcttttatga gaggcaggac acttaacgat gcatttgtta ttttagatga
     6901 agcacagaac actacatcta tgcagatgaa gatgttcctt acaaggcttg gatttaactc
     6961 aaaagcggtt attacaggag acgttacgca gatagatttg gagaaaaacg agacctccgg
     7021 gcttattcat agtgagaata tactaaagaa tatacccaat ttagactttg ttcatttttc
     7081 gaataaagat gtggttagac acccaattgt aaaaaagatc attgaagctt acgaaaaaga
     7141 agtttaaact aaaggaaata aatacttata ggggtgtgag tggagatgag aacaatttat
     7201 ataacattac cagtattaat aattgcgata tcatttttag caggctgcac aggaaaatgg
     7261 caaaatccca atgatactat tgcagctcag gaattaaaga taagaaatct gctaataatg
     7321 ccaaatattt acgaccatgc tggagtaata ttagagggca agatctggga tcttgatttt
     7381 gtagaggata aagaaaaccc ggataaggtc tacagcatgt tcaaactagc agataaggat
     7441 ggaaacttcg ttcatgtaac atctgaaggc atgacaacac ttttacaaga aggtgagata
     7501 gttaaagtaa ttggtataca tagggtgtta tacggattgg aaacaagaac gatttcttat
     7561 cttgtagat
//
LOCUS       DBNGSV010000349         7481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_548854, whole genome shotgun sequence.
ACCESSION   DBNGSV010000349 DBNGSV010000000
VERSION     DBNGSV010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_548854"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC159U_12460"
     CDS             complement(<1..449)
                     /locus_tag="AC159U_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337907.1"
                     /translation="MDSSEIAQKVNLLERNIQKVVKGKKEVIRDVIGALLSGGHVLIE
                     DVPGVGKTTLAQSLAKSTNLSYNRIQFTSDLLPSDILGVSIFDQNKTEFKFSPGPIFS
                     SIVLADEINRATPKTQSALLQAMSESKVTVDGKTYKLVQPFIVIATQ"
     gene            complement(472..3315)
                     /gene="secA"
                     /locus_tag="AC159U_12465"
     CDS             complement(472..3315)
                     /gene="secA"
                     /locus_tag="AC159U_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3337908.1"
                     /translation="MKKIVGSQNTRQLKKMQSVVESCTEYEQQIRVLPVSELSKKSDD
                     LRAKIKETINSNLDTDEDEFYNKLEDTLAEILPEAFALVREASKRTLGMRHFDVQLMG
                     GISLHNGNISEMKTGEGKTLVASLPLYLNGLSGYGAHLITVNDYLARRDAMWMAPIYK
                     ILGLKVGVINHEISYLIDWEDEQKAQKAIDENLSVWPAEFADLEIPPDRNLDALTSFK
                     TKLTECSRQDAYRADITYGTNNEFGFDYLRDNMKFSLEDYVQKEHNFAIVDEVDSILI
                     DEARTPLIISGPSEDSTERYYQIDSVVKKLKKEVDFTIDEKTKQVTPTEEGSNKVENI
                     LGITNIYDPANLELLHHVIQGLRAHHMFQNDVDYIVKEGQVVIVDEFTGRLMPGRRWS
                     DGLHQAIEAKEGVQIENENQTLATITIQNYFRMYRKLSGMTGTADTEAFEFKNIYKLD
                     VSVIPTNKDMIRDDQNDVIYKTEKEKLDAIVNEIREINDAGRPILVGTTSIENSEKIS
                     KHLERSKVKYNVLNAKQHEKEAHIVAQAGRLKAITIATNMAGRGTDIILGGNPEFLAA
                     DIAKKTYNAEMSELEDDEYEQVMIEAKNICDAEKEKVLELGGLHIIGTERHESRRIDN
                     QLRGRAGRQGDPGSSRFYVALDDDLMRIFASEKITSVMDKLGWKEGEPIEASLISKSI
                     ENAQKKVESRNFDMRKHLLEYDDVLNTQRDVIYTKRKEILSGENIKEIFYEILDETID
                     SIVLENIPQKAKEDEVDYEELSETFKRLFNLDITTDELSEYGLENDKITEQLKTRIKD
                     LYEKKEEEFGEETLRQIEKFIILQTLDTLWKDHLLSMDHLREGIGLRGYAQKDPLREY
                     RKEGYETFTGLLERFNEEVCNKLFLVQPVSDVDIERLERRRRIEQERMVIGRGEEEEK
                     KKPMPVRREKKVGRNESCPCGSGKKYKKCCGKG"
     gene            complement(3406..6894)
                     /gene="smc"
                     /locus_tag="AC159U_12470"
     CDS             complement(3406..6894)
                     /gene="smc"
                     /locus_tag="AC159U_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3337909.1"
                     /translation="MKLLKLEFNGFKSFYDKTKIDFHSNINAIVGPNGCGKSNIIDAI
                     KWVLGEQNPRNLRAMTMEEVISNGGETHKPLGMAEVSLVVSSNGSGYEEINVKRRLFR
                     SGQSEYYINNVPCRLKDITELFVDTGVGARAYSIIGQGKVESIITSKPEGKREVIEEV
                     AGIGKYKLRRKETQNKIELTKNNLTVVRESQSEILRQIKSLKRQAHDAEKYKQLAQEI
                     EEIDNKVLAKKKIQLEQSRTQSQSQQNDIGNDISRQNEILADLQKNREQTLSVIDGIE
                     KESDILKNKYFEDTKKLQTIDNEIELVNREERTLYETIATIKSDLINSLKEIENIKNT
                     TAQKHSELKSTLIILQEKQNELSVKQELTDSYKNDLVTKRQEYKDHREGLLKSLDEYG
                     SVKTKVVSIEKDVKELESRIYRLKKEKEDIQIKSALSTADLRYNENELKNIVSNKTVV
                     RSKLEVLSKELCMLVDSKKEVKSKLDNLRDQYNQAKSDLTAIEKIQSNYEWLPSDIGN
                     FILEHKGREVVGLMADFISVSENYDKAVEAALGEKLTWTIINNLNEAYDLIDLLRDQE
                     AGKGTFVPLDIYNNKSIHDTETLDLQPLVDVIEVDGQIKSLARNLLNKVYITESLNSI
                     NNLEQKLNSGYSFVTLSGDYIDCSGFISGGNSSHGVFERKREMEQLFQTTTKLNQSID
                     ELETELGSIEEKIESLTSEKINHESTLRQLDIREAEISKDIINLKNICRNYSQNYDSI
                     VFEVGNYEAERGNKVLSLSDLSKYINELELKKTRFETGFKNLDAELKELEDKDQHISS
                     YVNQGKVEIAGLKEKINGIEQELSYYLLRQKELGIKIIIQQSDFKILMYKKAGIVKGI
                     IDFGKDKVLLKEKIDIDHKNLNTISERKKEIEQQLAAYDTKISQVSSDLQTLNKSSTE
                     LDLALNSIDMELKHLLSDKDLNSLDYSSEHLDFDTEEMENKLLGLKNRLERFGPVNLL
                     APEEYLKLEDRNKFLTDQVEDLENAMESLRKAINKLDKESKTRFKETYDLVSEKFNEI
                     FPQIFKGGEGKLELTDPDDLLNTGVEVYVRPKGKKFQSINLLSGGEKALSAITLILSA
                     CFIKPAPFLLLDEIDAPLDDNNTRSFLEVVKEISKKSQILLITHNKNTMHDVNYLIGI
                     TSQEPGTSKVVSVELH"
     gene            complement(6979..>7481)
                     /locus_tag="AC159U_12475"
     CDS             complement(6979..>7481)
                     /locus_tag="AC159U_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3337910.1"
                     /translation="KREEYDSVSELDDLKSEAQILYQAEVSFNEGAALLKQKKYALAE
                     EKFNNAIQINPDNILYKGIIYWGRFLNNKDDSVNTDECVKELEKLISLDPNVAENYFY
                     LGNIYKHIDEFKKAENNFSKAVENDPDYTEAKRELRVLQNRLSEKKEARKSKTEKRFW
                     SSLFKK"
BASE COUNT         1876 a         1550 c         1160 g         2895 t
ORIGIN      
        1 ttttgggtag caattacaat aaaaggctgt acgagtttat aagtttttcc atcaacggta
       61 acctttgatt cactcattgc ctgaagcaga gcactctgtg tttttggtgt tgcacggttt
      121 atttcatcag caagaactat gcttgaaaaa atcggacccg gggaaaattt aaattcagtt
      181 ttgttctgat caaaaataga aacgccaaga atatcggagg gaagcaggtc gcttgtaaac
      241 tgtattctgt tgtacgagag gtttgtagat ttggcaagtg actgggcaag ggttgtttta
      301 ccaacacccg gaacatcttc aataagcaca tgaccccctg ataaaagggc gcctatcaca
      361 tcccttatta cctctttttt accctttact actttttgaa tatttctttc taacagatta
      421 actttctgtg ctatttctga actgtccaca ttaaatcctt tatttcaatt ttcatccctt
      481 accacaacac ttcttatatt ttttgccgct gccgcacgga catgactcat ttcttcctac
      541 ctttttctct cttcttacag gcataggttt tttcttctct tcttcttcac ccctgccaat
      601 caccattctt tcctgctcta ttcttctcct gcgctcaagc ctttcaatat caacatcgct
      661 tacgggctgt accaagaaaa gtttgttgca tacctcttca ttaaaccttt ctaacaagcc
      721 agtaaaggtt tcatatccct ctttcctata ttcccgtaga ggatcttttt gcgcataacc
      781 cctaagtcct attccctctc tcagatgatc catacttaga aggtgatcct tccaaagtgt
      841 atccagagtc tgcaatataa taaatttttc aatctgtctt aatgtttctt cgccaaactc
      901 ctcttccttt ttttcataaa gatctttaat cctggttttc agttgttcag tgattttgtc
      961 attctcaaga ccatattcac taagttcatc tgtcgtaata tccagattga atagtctttt
     1021 gaatgtttct gaaagctctt cataatctac ttcatcctct tttgcttttt gaggtatatt
     1081 ttctaaaaca atcgaatcaa ttgtttcgtc taatatttca taaaatattt cctttatgtt
     1141 ttctccagaa agtatctcct ttctctttgt atatataaca tccctttgag tatttagaac
     1201 atcatcatac tcaagcagat gttttctcat atcaaagttt cttgattcaa cctttttttg
     1261 cgcattttct atagacttag aaattagtga cgcttcaatc ggctcacctt ctttccagcc
     1321 aagtttgtcc atcactgatg tgatcttttc agacgcaaag attctcataa ggtcatcgtc
     1381 cagtgcaacg taaaatctgg atgatcccgg atcaccctgc cttccggcac gccccctgag
     1441 ctggttgtct atccttcttg attcatgtcg ttcggtgcct ataatatgaa gtccgccaag
     1501 ctctaggact ttttcctttt ctgcgtcgca aatgttcttt gcctcgatca tcacttgttc
     1561 atactcatca tcttcaagct cgctcatttc tgcattgtat gtttttttag ctatgtctgc
     1621 tgcaagaaac tccgggtttc cgccgaggat aatatccgta cccctgcctg ccatgttggt
     1681 tgctatcgtg atagccttta atctgcctgc ctgagcaact atatgagctt ctttttcatg
     1741 ctgctttgcg tttaatacat tatacttaac cttactcctt tctagatgct tgctaatctt
     1801 ttcagaattt tctattgaag tcgttccaac taaaattggt cttcctgcat catttatttc
     1861 ccttatttca tttactatag catcgagttt ttccttttcg gttttataaa taacatcatt
     1921 ttgatcatca cggatcatat ctttatttgt tggaataacc gatacatcga gtttatatat
     1981 atttttaaac tcaaaggctt cagtatctgc ggttcctgtc ataccagaga gttttctgta
     2041 cattctgaag tagttttgaa tcgtgatagt ggcaagggtc tgattttcat tttctatctg
     2101 cacgccctct tttgcttcta ttgcctgatg aagtccgtcg ctccaccttc ttccgggcat
     2161 gagtcttcca gtgaattcgt ctactattac cacctgtcct tctttaacaa tataatccac
     2221 atcgttttga aacatgtgat gagctctaag accctgtatt acatggtgca gaagctccag
     2281 attagccggg tcgtaaatgt tggtaatacc cagaatattt tctactttgt tagaaccttc
     2341 ttcagtcggg gttacctgtt ttgttttttc gtcaattgtg aaatccactt cttttttaag
     2401 ctttttaact acactgtcaa tttgataata acgctctgtg gaatcttcag aaggaccgga
     2461 aatgattaga ggagttcttg cttcatcaat taaaatactg tctacctcat ctacaattgc
     2521 aaagttatgt tctttttgaa cataatcctc caaagagaat ttcatgttgt ctcttaggta
     2581 atcaaatcca aattcgttgt ttgtaccgta ggttatatct gccctgtatg cgtcctgcct
     2641 agagcactct gtaagctttg tcttaaacga tgtaagcgca tccaggtttc tgtcaggggg
     2701 aatttctaaa tctgcaaatt ctgcaggcca tacgcttaga ttttcatcta ttgccttttg
     2761 agctttttgt tcatcttccc agtctataag gtatgaaatt tcatggttta tcacacctac
     2821 tttaagaccc agaattttgt aaataggagc catccacatg gcatcccttc ttgcaaggta
     2881 atcgtttacg gtaattaaat gggctccata tcctgaaagg ccgtttaagt atagcggaag
     2941 ggaagcaaca agtgttttac cctcacctgt tttcatctct gatatatttc cgttatgaag
     3001 cgatattccg cccataagct gaacgtcaaa gtgcctcata ccaagagtac gtttggatgc
     3061 ttctcttaca agcgcaaagg cttcggggag tatctcggca agtgtatctt caagtttatt
     3121 ataaaactcg tcttcatcgg tatcgagatt actgttaata gtttctttta tcttagccct
     3181 taagtcatcc gattttttgg aaagttcaga taccggaagc actcttatct gctgttcgta
     3241 ttcggtgcaa ctttctacaa ctgactgcat tttcttaagc tggcgcgtgt tttgagagcc
     3301 aactattttt ttcattataa aagatatcat gtattcactg tcctccgctt ttgcgcagtt
     3361 caaagtataa gcacttcttt ttgcataaca agggctttta atattttaat gaagttccac
     3421 tgatactact tttgatgtcc caggttcctg agaagtaata cctatcaggt aattaacatc
     3481 gtgcatagtg tttttattat gggttattag caatatttgt gacttctttg atatttcttt
     3541 aactacttct aaaaagcttc tggtattatt gtcatctagt ggggcgtcta tttcatccag
     3601 cagcaaaaat ggtgcgggtt taataaagca ggcagataaa ataagagtta tcgccgatag
     3661 tgctttctct ccacctgaaa gtagatttat agattgaaat ttcttgcctt ttggccgcac
     3721 atatacttcc acaccggtgt ttaagaggtc atcaggatct gttagctcaa gttttccttc
     3781 acctccttta aatatctggg ggaatatttc attaaatttc tcactcacaa gatcatatgt
     3841 ttccttaaat cttgtttttg attctttgtc cagtttgtta atagcttttc tcagagattc
     3901 catagcgttt tccaggtctt caacctgatc tgtaagaaat ttgtttctgt cttctaactt
     3961 tagatactcc tctggggcta aaagatttac cgggccaaac ctctcaaggc ggttttttag
     4021 ccccagcagc ttgttttcca tctcttcagt atcaaaatcc aggtgttcag aagaatagtc
     4081 caagctgttc aggtccttgt cagataggag gtgttttaac tccatatcaa tgctgtttag
     4141 agctaaatcc agctctgtgg atgacttgtt taaggtttgc aggtctgagg atacttgtga
     4201 tatttttgta tcatacgctg caagttgttg ctctatctct ttttttcttt cggaaatagt
     4261 gttcaaattt ttatgatcta tatctatttt ttcttttagt agtactttat ccttaccaaa
     4321 atcaattatg cctttaacga taccggcctt tttatacatt agaattttaa agtctgattg
     4381 ctgaataata atctttattc caagctcctt ttgtctcaat aaatagtagc tgagctcctg
     4441 ttctatacca tttatttttt ccttaagacc ggcaatttcc accttgccct gatttacata
     4501 agaacttata tgctgatcct tatcttcaag ctccttaagc tcagcatcca agtttttaaa
     4561 accagtttca aaacgtgtct tttttaattc cagttcattt atatatttac tcaaatctga
     4621 tagagacagt actttattac ctctctcagc ttcataatta cccacttcaa atacaattga
     4681 gtcataattt tgagaataat ttctacatat atttttaagg tttataatat ctttacttat
     4741 ttctgcttct cttatatcca gctgtcttag tgtagattca tggtttattt tctctgaagt
     4801 gaggctttca atcttttcct ctattgatcc cagttcagtt tccagctcat ctattgattg
     4861 attaagcttc gtagttgttt gaaaaagctg ttccatttct ctttttcttt cgaaaacacc
     4921 atgggaagaa tttccacctg atataaaccc gctgcagtct atataatcac cacttaaggt
     4981 tacaaacgaa tatcctgagt ttagcttttg ctctaaatta ttaatactat ttagcgactc
     5041 agtgatataa accttattca gtaaatttct tgctaaactt tttatctgtc catctacctc
     5101 aatcacatct actaagggct gtaaatcgag ggtttcggta tcatgtatag atttattatt
     5161 ataaatatcg agaggaacaa atgtaccttt tcctgcttcc tggtctctta gtagatcaat
     5221 caagtcatac gcttcattta ggttgttaat aatagtccag gttagttttt ctccaagcgc
     5281 tgcttcaaca gctttgtcat aattttcaga aacagagata aaatctgcca taagccctac
     5341 cacttccctg cccttatgtt ccaatataaa gttgccgata tcgcttggta gccattcata
     5401 gtttgactgg attttttcta tagcagtaag atcagacttg gcctgattat actgatccct
     5461 caagttgtct aatttagatt taacctcttt tttactatca acgagcatac acagttcttt
     5521 tgagagaact tccagtttgc ttctgacaac agttttattt gatacaatgt ttttaagctc
     5581 attctcattg tatctaagat cggctgtgga tagtgctgat tttatctgta tatcttcttt
     5641 ttcttttttt agtctgtaaa tccttgattc cagttctttt acatcttttt ctatacttac
     5701 tactttggtt ttgacacttc catattcatc aagcgatttt aaaaggccct ctctatgatc
     5761 tttatattcc tgtcttttgg taacgagatc gtttttatat gaatcggtaa gctcctgttt
     5821 tacagagagt tcgttttgtt tttcctgtag tatgattaat gtagatttta gctcagagtg
     5881 tttttgagca gtagtatttt taatattctc aatttcctta aggctgttta tgaggtctga
     5941 ttttatggtg gcaattgtct catacagagt cctttcttct ctgtttacaa gctctatttc
     6001 attatctatt gtctgaagct tttttgtatc ctcaaaatac ttgtttttta gtatatcgga
     6061 ttccttttcg ataccatcaa ttacactcag ggtttgttcc ctgttttttt gcagatctgc
     6121 tagtatttca ttttgtctgg atatgtcgtt acctatatcg ttttgttgag attgagattg
     6181 agttctagat tgttcgagct ggattttctt ttttgctaag accttattat ctatttcttc
     6241 aatttcctgg gcaagttgtt tgtatttttc tgcatcatgc gcctgtcttt taaggctctt
     6301 tatctggcgt aaaatttctg attgtgattc tcttactacc gtcaaattgt ttttagtaag
     6361 ctctatttta ttctgtgttt cttttcttct aagcttatac tttccaatcc cggctacttc
     6421 ttcaatgact tctcttttgc cctctggttt ggaagtgata atactctcca cttttccctg
     6481 tccaataata gaataagccc tggcacctac accggtgtcc acaaaaagtt ctgttatgtc
     6541 ttttagcctg caaggcacgt tattgatata atattcactc tgccctgatc taaatagcct
     6601 tctttttaca tttatttctt cataacccga tccatttgac gagacgacta aagatacctc
     6661 agccattcca agcggcttat gggtttcccc tccgtttgat atgacttctt ccatggtcat
     6721 tgctctcaag tttcttggat tctgctcacc aagcacccat ttaatggcat ctataatatt
     6781 tgactttccg catccgttag gtcctacaat tgcatttata ttcgaatgaa aatctatttt
     6841 ggttttatca taaaatgatt taaagccatt aaattcaagt tttaaaagtt tcatctacaa
     6901 ctatccttta tttttaaaat attatatagg caaatctgat atgtactgta taggaactct
     6961 ctgttttaaa cagaacggtt actttttaaa taaactcgac caaaatcttt tttcagtctt
     7021 tgattttcga gcttcttttt tctctgataa tctattttgc agtaccctta attctctttt
     7081 tgcttcagtg taatcgggat cattttctac agcttttgaa aaattatttt ctgctttttt
     7141 aaattcgtca atatgtttat aaatgttgcc aagataaaaa taattttctg caacattcgg
     7201 gtccagagat attaactttt caagctcttt aacacattca tctgtattta cactgtcatc
     7261 tttattattt agaaacctac cccagtatat tatacctttg tataatatgt tatcagggtt
     7321 tatttgtatt gcattattga atttttcttc tgcaagcgca tatttttttt gttttaaaag
     7381 tgcagcacct tcattaaagg atacctctgc ctgatacagg atctgagcct ccgatttaag
     7441 atcatcaagt tcactaacag agtcatactc ttctcttttc t
//
LOCUS       DBNGSV010000350        16518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1310814, whole genome shotgun sequence.
ACCESSION   DBNGSV010000350 DBNGSV010000000
VERSION     DBNGSV010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1310814"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="AC159U_12480"
     CDS             complement(<1..1015)
                     /locus_tag="AC159U_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3337911.1"
                     /translation="MAVYKKKNRWYIDYYLPNGDRKREVVTIEGVDSAQINRQDAIKA
                     LSIRKAEMAQGKFDITQTKKPVSIKQLCDEYFHQHSKENKRSWKRDRTTIKHFLEYFG
                     DKNIGQLNSFVITGWRNERSKQVKKATVNRELDTIRNMFNKAVEWGYIDRSPYTGVEK
                     YKVNNTNLRILSKQEFALLVDAATKQFKPLLLLAVNTGMRPNEMLSLKWEDVNLDDGY
                     LLVRDSKNYESRYIPIHKDLVRVLNELKSTCTNEYVFNGRKSYRKQWDKAFKLSGIKP
                     CRFYDLRHTFASNLVMSGVDIATVKELMGHKDISMTMRYSHPSPEHKKQAINGLNFTS
                     MDTY"
     gene            complement(1017..1304)
                     /locus_tag="AC159U_12485"
     CDS             complement(1017..1304)
                     /locus_tag="AC159U_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337912.1"
                     /translation="MSKNDKSIFDLGVKPEDMKYFNLHESTTAIEYLNLFRDRAKKIA
                     HNYFLLKDIQGKKVKIADLIDNKNTISNLFLIFKVFNHYTRLRFSIIKGKN"
     gene            complement(1728..1814)
                     /locus_tag="AC159U_12490"
     CDS             complement(1728..1814)
                     /locus_tag="AC159U_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR3337913.1"
                     /translation="MVIINVVVTDVMASNGVIHVIDTVVIPK"
     gene            2226..2687
                     /locus_tag="AC159U_12495"
     CDS             2226..2687
                     /locus_tag="AC159U_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025185.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HIR3337914.1"
                     /translation="MIGNFIEIIFLLLNRSVPPIHNKPFLVKNITQFWSNGWNIWVRD
                     WLFEITPKSIRKTKYLNIVFPFLISGIWHEIIINIPLFIITKINIFGTMTLYFLLQSL
                     GVITDKKFFRYKNDHNFRYIFCLLVAIGPSPLFFNDQLLLVFFFDLVSARN"
     gene            complement(2684..3190)
                     /locus_tag="AC159U_12500"
     CDS             complement(2684..3190)
                     /locus_tag="AC159U_12500"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HIR3337915.1"
                     /translation="MNILKQYQDQNSKSLDEMFNDTTLLLVFLRHNGCIFCRKTLQDL
                     SKIHDELTVKQVELVLVHMTTDDSARKLFRKYGLENVSRISDPQKNLYNHFELKKGSL
                     GQLFGPKVWLRGVSSLFEGHFIGKLEGDGFQMPGVFLIKDREIVKSFVYKTAADKPNY
                     LELAKCEL"
     gene            3726..5213
                     /locus_tag="AC159U_12505"
     CDS             3726..5213
                     /locus_tag="AC159U_12505"
                     /inference="COORDINATES: protein motif:HMM:NF016158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3337916.1"
                     /translation="MNKDRLIVLPGNCLFHHHEDLVPCDKDLFFLAEDYGLCTKYNYH
                     KHKLILILAAMRSYRDELQKKYSVEYFELNCDTKNSSYQQKILNTLNTYEIKKVVIYD
                     NQSRYFIKNIEKICRAKQLELTIKPPPMFLTSKEEFYEYRSSYKRLLMNDFYIWQRKR
                     LNILVDETKRPKGGKWNFDSDNRKKLPRNIKIPQIPKLNKKKHVLEVSDLINRFFPNN
                     PGSTENFILPTTRKQSLIWLNDFLEYRLIEFGPYEDAISKEDNFIYHSLLAPLLNIGL
                     LLPTEVITSALDYCSSNKVPIQSIEGFIRQIIGWREFINGVYNTEDFSGNYFNHKNKL
                     NSKWYKGGTGVGPLDYVIDKVIKYGYCHHIERLMVLSNIMLLSEINPVEVYRWFMEMF
                     VDSYDWVMVPNVYGMGQFADGGKFATKPYISGSSYILRMSDFKRGEWCEIWDGLYWRF
                     IDKNKNLFSQNKRMSLMVRLLQNIEQDKKRNMIKKAESFIEEVVL"
     gene            complement(5296..9693)
                     /locus_tag="AC159U_12510"
     CDS             complement(5296..9693)
                     /locus_tag="AC159U_12510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3337917.1"
                     /translation="MTLDNFNVTTKNWFEKNFKSPTEAQRLSWPSIQKGKHTLISAPT
                     GSGKTLAAFYISIDKLIKQSINDQLSQETQILYVSPLKALSNDIHKNLQIPLEGIKKE
                     MLLLGLSEPDIKISVRTGDTTQNERRQMIASPPHILVTTPESLYLLLTSVKGREMLSK
                     VNTLIIDEIHALLGSKRGSHLSLSIERLESLVHYPIQRIGLSATQKPIERIAKYLVGN
                     SNIIKNKPQCSLIEVKHKRKFDLTVEVPNSPLTAVMSNKVWSEIYDKIVKLINNHNTT
                     LIFVNTRRLAERLALALSEKMEKEEVTSHHGSMSKEHRLKAEQNLKSGKLKVLIATAS
                     MELGIDIGSVDLVVQISSPKSISNFLQRVGRSRHYVGGIPNGVIFPLSRDDLVECIAL
                     IRSVQQGELDEIKMPEKPLDILAQQIIAELSSKEWNIDKLYMLIKNSYSYRNLCVQEY
                     QDIIEMLSKGYTPRRGRKTAHIHYDAINSRIKARKGARLIAITNGGAIPDMFDYEVVM
                     HPEGTIIGSVNEDFALESTPGDIFTLGTHSWQLLRVEGLKILVKDATGIPPTVPFWFG
                     EGAGRTIELSESISKLKNQISKLLDEDPLQTDSENMFSKIKVNKAVRWLQEEIGIDNS
                     AAEQAIIYLYNGMKGLGDIPSLDTIIIERFFDDAGDMHVVIHSQFGSRVNKAWGLSLR
                     KKFCRKFNFELQAAANDDSIILSLSSSHSFPLEEVFNYLNKKTIRDTLIQAMLDAPMF
                     DVRWRWNATIALAIQRNRAGKRVAPQLQRMQSEDLIAQVFPDQIACFENIDGQREIPD
                     HPLVAQTINDCLTEAMDIDMLENILLNIEKNNIDLISRDLREPSPFAQEIINARPYAF
                     LDDTEFFERRVNAVRTRSWLDPSEATELSNLDPESIKHVKNEAWPQAKSADELHYALS
                     IYGYITSTEIINNEWGHILELLRFDSRAFCIITTNCKKVDNDDKRIWIATERFAQFRT
                     IFPHIENTFEYNIPDFISNQNWSFEEALKEIIRGRLEALGPVCINRFIEETGITENNI
                     KSALAALESEGFVFRGHFTNKKGNEEWCERRLLQRIHIYSIDSLRESIKPVSVQDFMR
                     FLFFHHGVTENNEMQTGLSSLEKTLRQLEGYVSAAVSWERDIITTRIKDYDPEWLDIL
                     CTSGKFTWGRLEPPKTKGKGSPSPVKRTPISFMRRENLELWNLLSSVNISNFDYTSST
                     KLIMEYLDNNGASFFNEIIKNTSLLKSQVQQGLAELVAYGAVSCDNYSGLRALLTPGY
                     NSNRSNSRRRKKSPVYGIENAGRWTLMHKNNTNTEIEYDYKDLEKLALIFIDRWGVVF
                     RTILENESFAPPWRILVRVLRRLELKGVLRAGRFISNVSGEQFAYPETVVNLREIKNK
                     IMTGQLVSISAADPLNLIGSILPGDKVPNLIKNRILYRDGIPIAVLEGIETKFLKEFS
                     ENQKWELKNALIRRAFPPKLKAYLGRNYK"
     gene            complement(9916..10452)
                     /locus_tag="AC159U_12515"
     CDS             complement(9916..10452)
                     /locus_tag="AC159U_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR3337918.1"
                     /translation="MIIKILIIFIAISTNCFAENATSLNTVKRVDLKKYKGTWYEIAK
                     IPNRFQKKCVSDTTATYELYDDSRINVVNKCFDKNGKLLVAKGIAKIVDTNSNSKLKV
                     SFFSIFGWYLFWGDYWIIELGDDYDYSIIGHPDRKYGWILSRTPKLLKKKEEILSILE
                     DNGYNPDEFEFTKQDTKY"
     gene            complement(11150..11821)
                     /locus_tag="AC159U_12520"
     CDS             complement(11150..11821)
                     /locus_tag="AC159U_12520"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR3337919.1"
                     /translation="MARILKDAENVFMGVASNLPFFSIWLAKQLYNPDLTWLNIPGKI
                     NPSPNKPPKSTVDFEFIKGSSSSITLASIFDMSARGELDVAFLSGVQIDKFGNFNLSH
                     IPDGDRIKIQFTGGAGSALICATTKRVIFWRTKHDKRAFVEKCPINTATGNVYKAVSP
                     LSVFAKQDGVLQLESINPNSSFKEVSDNTGFPVYEAPYTEPPTEDELGLLRDFDKEGI
                     RNIEF"
     gene            complement(11879..12673)
                     /locus_tag="AC159U_12525"
     CDS             complement(11879..12673)
                     /locus_tag="AC159U_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR3337920.1"
                     /translation="MNKIKSLEESAKLVKDGDRLAIGGLLLQRTPSAFVRELARQGKK
                     NLKLMKTAANYDFDLLCLAGCTDEITAGFVSFEAEFGLAPNFRKSVESEQVKFNENSC
                     YTVISSLRAAAFGVPFMPIADLKESYLVDKFKSVDNPYADNGNNQILTVPAIKPDWAV
                     LHVQKADEHGNAIIYGPVFEDLIMAQAASKIILTTENIVEENEIHKELDLAVIPGFKV
                     QAVVEASGGAKPASCYPYYDYNADDVKNYLSIKDQSELVSYLENHI"
     gene            12842..13507
                     /locus_tag="AC159U_12530"
     CDS             12842..13507
                     /locus_tag="AC159U_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HIR3337921.1"
                     /translation="MSAKIEPLKIKDCDLLAIATGQRAQNLKELRNNLLTCHPGCIYN
                     HFWGVLVRPGFTDPEFNNDFAQWASHSIHDIKLAETLGVLDPTDFKYIEDLRSQLIEI
                     IEQRLDESEYLTWSHSDRQFNFVRAEIVVFDTKQTIKKPQHLPRVVSNFSVNSIFYHF
                     IDARRRSAQGEDDIRAWLLTIDGEYKGLCDKLAQLDPYFVTLTELRNHITQILKTYFK
                     KIN"
     gene            13507..14748
                     /locus_tag="AC159U_12535"
     CDS             13507..14748
                     /locus_tag="AC159U_12535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3337922.1"
                     /translation="MGTLLDSYAEVVGKNVIDHLRQLSEPLKTKRVIHVNSTRYGGGV
                     AEILHQLIPLMKELDIDTSWEVIEGNEKFYKCTKSFHNALQGDAVDIPEVLLKNFEKT
                     NRQNAKQLQSTLKDADIVFIHDPQPAPLLNFIPQRTGKWVWRCHIDASRPYRPVWKYL
                     RQFVSGYDASIFSIADFAQPLPHTQYLIPPSIDPLSDKNRELDDPQINAVAKKFKIDR
                     TRPIMLQVSRFDRFKDPVGVIKAYKHTKSFLPNVQLILAGGTADDDPEGAEVLAEVRQ
                     EAGNDKDIHILLLPPDAHIIINALQRIADIILQKSVKEGFGLTVTESLWKGTPVIGGD
                     TGGIRLQVIDHHTGFCVNSPEGAALRIRYLLHNPEKIKEMGSKAKEFVRENFLITRHL
                     QEHLTLMVKLLYNNSSDRIEI"
     gene            14824..16206
                     /gene="argH"
                     /locus_tag="AC159U_12540"
     CDS             14824..16206
                     /gene="argH"
                     /locus_tag="AC159U_12540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3337923.1"
                     /translation="MFKKPWGGRFKKQTDKLMEKFSASIDYDRNLYAEDIEGSIAHAK
                     MLSATGIIKKTEADKIIRGLGSIKKEIESGKFPFRDEFEDIHLNIEKRLIEKIGAVGG
                     KLHTARSRNDQVLTDEKLYIRKQISEIKSRLKKFVGNIVKLAEKNSDVVIPFYTHLQR
                     AQPVYLSHYLLSYFEMVNRDKQRLDDCLERLNVLPLGAGAGAGTSFPIDRKLVARLLD
                     FPEVSKNSIDTVSDRDFIIEFLSDSSILMMHLSRMCEDFIIWSSKEFDYIDLGDDFTT
                     GSSIMPQKKNPDACELVRGKIGRVYGNLFSLMTIMKGLPLSYNRDMQEDKEQLFDTVD
                     TVKSSVELISEMISGIKFKKDNIAGSLKGGFMTATDIADYLARKGMPFRDAHEVTGNI
                     VSYAEKKESELEDLTLDEYKKFSKLIDKDIFSCITIEGSANSRTSYGGTAKKNVDKML
                     REAKKEVAKW"
     gene            16200..>16518
                     /locus_tag="AC159U_12545"
     CDS             16200..>16518
                     /locus_tag="AC159U_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337924.1"
                     /translation="MVKKISILVLALLLALVVIEKVVLRKKNTPYEFWRFNGVYKLDD
                     DLIYSHKINSKRSWRFKDNDRTITEVVSINNLGLRDREFNKNEEFDKRILILGDSMTF
                     GHGV"
BASE COUNT         5233 a         2864 c         2829 g         5592 t
ORIGIN      
        1 aataggtgtc catagaagtg aaatttaggc catttatagc ctgtttttta tgttcaggtg
       61 atggatgtga atatctcatt gtcatactga tatctttatg ccccatgagt tctttaactg
      121 ttgctatatc tacgccagac attactaaat tacttgcgaa agtgtgcctg agatcataga
      181 acctgcaagg ctttattcct gataatttga aagctttatc ccattgtttt ctgtaagatt
      241 ttctaccatt gaacacatac tcatttgtgc atgtactttt taattcattt aacactctta
      301 ctaaatcttt atgaatagga atataacgac tttcataatt tttactatct ctaacaagaa
      361 gatagccatc atccaaatta acatcttccc actttaatga tagcatctca tttggtctca
      421 tacctgtatt tacagctaat aataacaatg gtttaaattg ttttgtggcg gcatccacaa
      481 gtagggcaaa ttcctgtttg cttaatatcc ttaagtttgt gttattgact ttgtatttct
      541 caacacccgt atatgggctt ctatcaatat aaccccattc aacagcttta ttgaacatgt
      601 tgcggatggt atcaagctct ctgttcaccg tagctttctt aacctgctta gagcgctcat
      661 ttctccagcc tgtaattaca aatgagttta actgccctat atttttatca ccgaagtatt
      721 caagaaaatg tttgattgtt gttctgtctc ttttccatga ccttttgttt tctttagaat
      781 gttggtgaaa gtattcatca cacaactgtt taatagaaac aggtttttta gtttgagtga
      841 tatcaaactt cccttgtgcc atttcagcct ttctaatgga caatgccttg attgcatcct
      901 gtctattaat ctgtgcagag tctacgcctt caatagttac aacttccctt ttcctatcac
      961 cattaggcaa ataataatct atataccaac gatttttctt tttatatact gccatattaa
     1021 tttttcccct ttattatgct aaaccttaat cttgtatagt ggttaaatac tttaaatatt
     1081 aaaaataaat tgcttatagt atttttatta tctattaaat cagcaatttt aacctttttg
     1141 ccttgtatat cttttaataa aaaataatta tgggctattt ttttcgctcg atctctaaac
     1201 aaatttaagt attcgattgc tgttgttgac tcatgtaaat tgaaatattt catgtcttcg
     1261 ggcttaactc ctaaatcaaa aatgctttta tcatttttag acatttgatt ttcttgagtt
     1321 taactttggc tcttttaaat ctataccttc ttgttgacct tcccccctat aatagtacca
     1381 gattacaagt tagaatttta ggtaataaac cctatgtata ggaggcaacg gaaatgccta
     1441 ggaagcgtta cacaccagag cagataataa gatatttaag acatgcagag gttcttagtt
     1501 cccaggagaa aagctattta tttccaatta attaggtgtg aatcaaatta tccacttgtt
     1561 gcaaattaga tgtatcttat aggactgcta tctgtagata gaccttatat tcggaatctg
     1621 aaaaattact taaagctggg atgtctataa tagtaaatgc ttatggtgat aatcccattc
     1681 aaacatattt gtttataaag gccggtactg gaccggcctt taacattcta ttttggaata
     1741 actactgtat caattacatg aataacacca tttgaggcca tcacatcagt aacgactaca
     1801 ttaataataa caatcggtaa gcattaaaat attatatata ttatttattt tagataagtt
     1861 tgggctatat ttgcattaag tagaaaatat taaatatgaa tagtttcaaa tttgttgcat
     1921 actttttaat catatatttt gtttgtggtg tactgattaa aacttgcttg ttatattcaa
     1981 caacatactc tacaagaaat atatatagat tcttatttct ttctcccttc tattcaacta
     2041 aaacttggag caaaattaaa acggtaaatg gtcctaatag attaggaaat ttgaaaaaat
     2101 ttatgtattt gctcactttg tttactttag ttttagtgtt ttataaaatc cttacgagca
     2161 atttatatga ttatcactac ttaattgtgc tgatatctat aatacctttt tatcttataa
     2221 cagagatgat tggtaatttt attgagataa tttttcttct acttaatcga agtgtgcctc
     2281 caatccacaa taaacctttt cttgtgaaaa atattacaca gttctggagc aatggttgga
     2341 atatatgggt aagggactgg ctatttgaga taacaccaaa gagtataaga aaaacaaaat
     2401 atttaaatat tgtttttcca tttctcattt caggtatatg gcatgagata attattaata
     2461 taccactttt catcatcact aagattaata tatttggaac tatgacacta tattttttat
     2521 tgcaatcctt gggtgttatt acagacaaaa agttctttag atataaaaat gatcacaact
     2581 tccgctatat attttgctta ttagtagcta tcgggccttc gccattgttt tttaacgatc
     2641 aattactcct agtattcttt ttcgatttgg tttcagctag aaattagagt tcacatttag
     2701 ctaattcaag gtaatttggc ttatccgcag cagtcttgta tacaaaactc ttcacgattt
     2761 ctctatcttt tattaaaaaa acacctggca tctgaaaacc atctccttcc aacttgccta
     2821 taaaatgtcc ttcaaataag gaacttacgc ctcttagcca tactttaggt ccgaataact
     2881 gtcccaaact tcctttctta agttcaaagt gattatatag attcttttga ggatcagaga
     2941 ttcgagatac attttccaag ccatattttc tgaacagttt cctagcagaa tcatcagttg
     3001 tcatatgcac tagaactaac tctacttgct tcactgttaa ttcgtcatgt attttagaca
     3061 gatcttgtag tgtctttctg caaaaaatac atccattatg tcttaaaaaa acaagcaata
     3121 acgtcgtatc attaaacatt tcatctaatg atttactatt ttgatcctgg tactgtttta
     3181 atatattcat gtaatggttt gaattacttc aattgatgtt cgaattaact aataaagtta
     3241 aaatataaat aacaataacg cttcttactg tccaacaaat agtacgaatc caattagtat
     3301 caaccaattt cttaaattga gtataatcaa atccattaga taaactccca tgatatttca
     3361 cctgcaacaa aaaagttgat atccatatca atagtactaa tacaaagcta actataaagt
     3421 aatttgtagg aattgaatct gggtgaaatt ttattaataa gccagaagag ataagttcga
     3481 ctaacattgg aaatattact ataaagctta tattcttaga gtgtaaatta tgaaattcgt
     3541 agaaattatt ccttgataca taagtaaata atggataatg aaccacttgt actacccata
     3601 tgacaccgag cataaaaata gtagatatca cgtttgtaag cactaaaatt gtctccataa
     3661 acgaaaaggt tagcctataa tgttttatag tcatgattac gcattatttt taactaaatt
     3721 ctaaaatgaa taaagataga ttaattgtat tacctgggaa ttgcttattc catcaccatg
     3781 aggatcttgt gccgtgtgat aaggatttat tcttcttagc tgaggattat gggctttgca
     3841 caaaatacaa ttatcataaa cataaattga tattaatcct tgctgctatg agatcgtaca
     3901 gggacgaact tcaaaaaaaa tatagcgtcg aatatttcga gttaaattgt gatactaaaa
     3961 atagtagtta ccaacaaaaa atattaaata ctttaaatac atatgaaata aaaaaagtag
     4021 taatttatga caatcaaagt cgttacttta taaaaaatat tgagaaaata tgtagagcga
     4081 aacaattaga attaaccata aagccccctc ccatgtttct tactagtaag gaagaatttt
     4141 atgaatacag gagttcttat aaaagacttc tcatgaatga cttttatata tggcagcgaa
     4201 aaagacttaa catattagtt gatgaaacta aaagaccaaa aggtggaaaa tggaacttcg
     4261 atagtgataa cagaaaaaaa cttcctagaa atattaaaat tccccaaata cctaaattaa
     4321 ataaaaagaa acatgtatta gaggtatcag atttaataaa tagatttttc cccaataatc
     4381 ctggtagtac agagaatttt attttaccaa ctacaagaaa acagtcttta atctggttga
     4441 atgatttctt ggagtatagg cttatagagt ttggacctta tgaagatgct atatccaaag
     4501 aagataattt tatttatcac tcattattag ctcctctttt aaacataggt ttgttgcttc
     4561 ccactgaagt tattacatcc gccttagatt attgtagttc taataaagtg ccaattcaaa
     4621 gtattgaagg ttttataaga cagataattg gctggagaga attcattaat ggtgtttata
     4681 acactgaaga ttttagtggg aattatttta atcacaaaaa caaactaaac agtaaatggt
     4741 ataaaggtgg cactggagtt ggtccattag attatgtgat tgataaagtc ataaaatatg
     4801 gatactgcca tcatatagaa aggttaatgg ttttgagtaa tatcatgctt ttatctgaaa
     4861 taaatcctgt tgaggtttat agatggttta tggagatgtt tgtagattca tatgattggg
     4921 taatggttcc caatgtgtat ggaatgggcc agtttgcaga tggaggaaaa ttcgcgacaa
     4981 aaccatatat atcgggttca tcttatatac tcagaatgag cgatttcaaa agaggtgaat
     5041 ggtgtgaaat atgggatgga ttatactgga gatttattga taaaaataag aacttatttt
     5101 cacaaaataa aaggatgtca ttaatggtaa gattgcttca gaatatagag caagataaaa
     5161 aacgaaatat gataaaaaaa gctgaatcat ttattgaaga agttgtttta taaataagta
     5221 gatatacaaa ctaattaatt aactttaaac acttatgcga tatttctgat gatattagta
     5281 atataactct aaatattatt tgtaatttct tcccaaataa gcctttagct taggtgggaa
     5341 tgctcttcgt atcagagcat ttttaagctc ccatttctga ttttcagaaa attcttttaa
     5401 aaattttgtt tcaatacctt ctaaaactgc aattggaatc ccgtccctat ataatatgcg
     5461 atttttaatg agattaggca ctttatctcc aggcaaaatt gagccgatta ggtttaatgg
     5521 atctgcagct gagatagaaa ctaattgccc tgtcattatt ttgttcttga tttcgcgtag
     5581 atttactaca gtctctggat aagcaaactg ctctccactg acatttgaaa taaaacgtcc
     5641 agctcttaaa acacctttaa gctctaatct cctgagcact cttaccaaaa ttctccatgg
     5701 tggagcaaat gattcatttt ccaatattgt cctgaaaaca acaccccaac gatcaataaa
     5761 tattaatgct agtttttcta aatctttata atcatattca atttcagtat tggtattatt
     5821 tttgtgcatt aaagtccaac gtccagcatt ttctattcca taaacagggg attttttcct
     5881 tcttctggaa ttacttctat tactgttata tccaggtgtg agtaatgctc tcaggccgga
     5941 ataattgtca caactaacag caccgtaggc tacaagctct gctaaacctt gttggacttg
     6001 gcttttaagt aaagaggtgt ttttaattat ttcattaaag aagctagcac cattattatc
     6061 caaatattcc attattaact ttgtagagct tgtataatca aaattggaaa tatttacgga
     6121 agaaagcaaa ttccataatt ctagattttc tcttctcata aatgatatgg gagttctctt
     6181 tacagggcta ggggatcctt ttccttttgt tttgggtggt tctaaccggc cccaagtaaa
     6241 ttttccactt gtacaaagta tatctagcca ttctggatca taatctttta ttcgtgtggt
     6301 tataatatct ctttcccaac ttactgcagc agatacataa ccttctagtt gtcttaatgt
     6361 tttttcaaga gatgatagtc ctgtttgcat ttcattgttt tcagttactc catgatgaaa
     6421 gaaaagaaaa cgcatgaaat cttgaacact tactggttta atactttctc ttaaagaatc
     6481 tattgaatat atatgtattc tttgtaagag tctcctctcg caccactctt cattaccttt
     6541 cttattggta aagtgtcctc taaaaacaaa gccttcactt tcaagagcag ccaaggcact
     6601 tttaatatta ttttccgtaa taccagtttc ttcaataaat ctgtttatgc agactggacc
     6661 caacgcttca agtctacctc ttataatctc ctttaaagct tcctcaaaac tccaattctg
     6721 atttgatatg aaatctggaa tattatattc aaaagtattt tctatatgtg ggaaaattgt
     6781 tctgaattga gcaaaacgtt cagttgcaat ccaaattctc ttatcatcat tatctacttt
     6841 tttacaattt gttgtaataa tacaaaatgc tcttgaatca aatctgagta attctagaat
     6901 atgtccccat tcattattta ttatttcagt agaagttatg tatccatata tgctcaaagc
     6961 ataatgaagc tcatcagctg attttgcttg tggccatgcc tcatttttga catgcttaat
     7021 agattctgga tcaagattac tcaactcggt tgcttcagaa ggatctaacc aacttctagt
     7081 tctaaccgca ttcacacgcc tctcaaaaaa ctcagtatcg tctaaaaatg catatggtct
     7141 agcatttatg atctcttgtg caaacggtga tggttcacga agatcccttg atataagatc
     7201 aatattattc ttctcaatat tcagcaatat gttttctagc atatcaatgt ccattgcttc
     7261 agttaaacag tcatttatag tctgtgctac cagtgggtga tcgggtattt ctctttgacc
     7321 gtcgatattt tcaaagcaag ctatttgatc tggaaatact tgtgctataa gatcttccga
     7381 ttgcattctt tgcagttgcg gagctacgcg tttgcccgct ctgtttcttt gtattgctaa
     7441 agcgatagtc gcattccatc gccagcgcac atcaaacatt ggtgcatcta acattgcctg
     7501 aatcaaagta tctctaatag ttttcttatt tagataatta aatacttctt caagcggaaa
     7561 gctgtgtgat gagcttaatg acagtattat actgtcatcg tttgctgcag cttgaagttc
     7621 gaaattgaat tttcgacaaa atttcttcct taaggacaag ccccatgctt tatttactct
     7681 gcttccaaat tgagaatgta ttacaacatg catatcacca gcatcatcaa aaaatctctc
     7741 gattattata gtatctaatg atggaatatc tcctaaccct ttcattccat tatataaata
     7801 tattatcgct tgttctgctg cagagttatc gatgccgatt tcttcctgta gccagcgcac
     7861 agctttattg acttttatct tactgaacat attttccgaa tctgtttgca atgggtcttc
     7921 atctagaagt ttagaaatct gattctttag ctttgaaata gattcgctaa gttctatagt
     7981 ccgtcctgca ccttcaccaa accagaacgg cactgtaggt ggtattccag tagcatcttt
     8041 tactaggatt ttcaagcctt caacacggag aagctgccaa gagtgtgttc caagagtaaa
     8101 tatatcacct ggtgttgatt ctaaggcaaa atcctcattt acacttccaa ttattgttcc
     8161 ttcggggtgc atgactacct cataatcaaa catatcgggt atagcaccgc cattcgtaat
     8221 tgcaattaat cttgcacctt ttcttgcttt gatcctcgaa tttattgcat catagtgaat
     8281 atgtgcagtt tttctacctc gtcgaggtgt atagcctttt gaaagcattt ctatgatatc
     8341 ttgatattcc tgtacacata aatttctata tgagtaagaa tttttaataa gcatgtacaa
     8401 cttatcgata ttccattctt ttgatgatag ctctgcgata atttgctgcg ctaatatgtc
     8461 aagcggcttc tcaggcattt taatctcatc taattctccc tgttgcacac ttcttataag
     8521 tgctatgcat tcgacaagat catctcttga aagtgggaaa atgactccat taggaatacc
     8581 tccaacatag tgacgacttc ttccaactct ctgtaagaaa tttgaaatgc tttttgggga
     8641 tgatatttga actactaaat cgaccgaacc tatatcgatt ccgagctcca tggatgctgt
     8701 agctattagc acttttaatt ttccggattt aagattttgt tcagctttta gtcgatgttc
     8761 tttgctcata ctgccatgat gagatgtaac ttcctctttt tccatttttt cactgagtgc
     8821 tagagcaagt ctttcagcta atcgcctagt attcacaaat atcaatgttg tattatggtt
     8881 attgattaat ttaacaatct tatcgtagat ctcactccag actttattgc tcataacagc
     8941 agttagtggt gaattaggaa cttctactgt taggtcaaat ttacgcttat gttttacttc
     9001 aatcaaacta cattggggtt tgtttttaat tatattagag ttcccgacca gatatttcgc
     9061 aatcctttct ataggctttt gagttgcaga aaggccaatc ctttgaatag ggtaatgaac
     9121 taatgattca agtctttcta ttgagagtga taaatgagaa ccacgcttac tgccaagaag
     9181 tgcatgtatc tcatcaataa ttagagtatt aacctttgag agcatttccc tgcctttcac
     9241 acttgtgaga agcaagtata aagattctgg tgttgtaact aaaatatgag gaggactagc
     9301 aatcatttgc ctcctttcat tctgtgtcgt gtctcccgta cgaaccgaaa ttttgatatc
     9361 tggttcgctt aatcctaaga gcaacatctc cttttttata ccttctagtg gtatttgtaa
     9421 atttttatgt atgtcattgc tcagggcttt taaaggggaa acatatagaa tttgtgtttc
     9481 ttgagaaagc tgatcgttta tgctctgttt tattaattta tctattgaaa tataaaaagc
     9541 tgccaaagtc tttccactac ccgtaggtgc cgaaataagt gtgtgtttgc ctttttgaat
     9601 tgatggccat gaaagccgct gagcttcagt cggactttta aaattttttt cgaaccagtt
     9661 tttagttgta acattaaaat tatctagagt catatattta gaattattat tcactcaaat
     9721 tagcgatatt aaagatttta aaattaagct agtcaaaatt ttactatatc ctaaacatag
     9781 gcaaattttg tctacattat ttagaaaata tttaagttag gttgatttag ataatgcaaa
     9841 agtttgacat gattataata taagtattgt gagaaaagaa tcggagacat agtttttata
     9901 ttttctgaag gaaaattaat atttcgtatc ttgttttgta aattcaaatt cgtctggatt
     9961 gtaaccatta tcctccaata ttgacaaaat ctcttccttt ttttttagta gttttggtgt
    10021 gcggcttaat atccacccat attttctgtc tggatggcct atgattgaat aatcgtaatc
    10081 atcgccaagt tctattatcc aataatcccc ccagaataag taccaaccga atatactaaa
    10141 aaaacttact ttgagttttg agtttgaatt cgtatctact attttggcaa taccttttgc
    10201 tactaaaagc ttaccatttt tatcaaagca tttattaact acattaattc tgctatcatc
    10261 atacaattca tatgttgcag tagtatcgga aacacatttt ttctgaaagc gatttgggat
    10321 tttagcaatt tcgtaccaag ttcctttgta cttttttaga tcaacccttt ttaccgtgtt
    10381 taaagaagta gcattttcgg caaaacaatt tgttgaaatg gctatgaata taattaatat
    10441 tttgataatc atattataaa ttataccctt tttagaaact tattatgagt actaatcgaa
    10501 agagattctt ggctacggaa ttggctaggg ttgactacac tttataactt ataattacac
    10561 ttaataacgc tcatagtatt taattatata gttgccaaaa tgcctgaaaa tatgggcatt
    10621 tctcagctta aaaaatctat ataaatttag cccaaattaa ttcagtagcg cacgaaaacc
    10681 cgcatccttg cggaattggg gcttcgaatc ccatgtcctc tgctttcctt gcattctggt
    10741 atgaacagaa actaacaaga tgaaattatg gattaatgaa agtattgtag atatagaata
    10801 aaaaccatac tcaacataca cacacaaatt aatacattgc ggataatttt acaagataaa
    10861 tatggggaca tatttgctaa gtaaattttg aactaggtgt ccaaataggt gtccatatag
    10921 tgctagtttg gagcattttg tagagtttgg tataacatca ctaattctgt aaaattataa
    10981 tgtttagaag tagttaggtg ttgtatgaat ttgtaagttt tactgatctg aaagcgtccc
    11041 gaacgcaacg ctctaccaaa ctgagctaca ccccgattta cgtacaattg caaacagatg
    11101 ttattgcgat attgatttaa atataatgct tttaagattc cttatccact caaaactcaa
    11161 tatttcttat tccttcttta tcaaaatctc ttagcagtcc tagttcgtct tctgttgggg
    11221 gttcagtgta tggagcttcg tagaccggaa agccagtatt atctgaaact tctttaaagc
    11281 tgctatttgg gttgatactt tctaactgca gtactccgtc ctgttttgca aaaactgaca
    11341 gtggtgacac tgctttgtag acattaccgg tggctgtgtt tatggggcat ttttcaacaa
    11401 atgctctttt gtcatgtttt gtgcgccaga atattaccct ctttgtcgtg gcacatatta
    11461 gcgcgcttcc tgcgccgcct gtaaactgta tttttattct atcaccgtct ggaatgtggc
    11521 ttaagttaaa gtttccaaac ttgtctatct gcactcctga tagaaaagcg acatcaagct
    11581 ctccccttgc agacatatca aaaatacttg caagcgtaat agatgatgag cttcctttta
    11641 taaactcaaa atccacagta gatttgggtg gtttgttggg ggagggattt attttgccag
    11701 gaatattaag ccaggtcaga tcagggttat atagctgttt tgcaagccag atagaaaaaa
    11761 aaggcaggtt agaggcaact cccataaaga cattctcggc atcttttaat attcttgcca
    11821 tagatacaat catatattca gccggcgata tttgaagggt aatattaata gtcatcaatt
    11881 atatatgatt ttccagatag ctaacaagct cagattggtc ttttatagaa aggtaattct
    11941 ttacatcatc tgcattataa tcatagtagg gataacatga agcgggtttt gcccctccag
    12001 aagcttctac tactgcttgc actttaaagc ccggtatgac agcaaggtcc aactctttat
    12061 gaatctcatt ttcttccaca atgttctcag tggttaatat aatcttactt gctgcctgag
    12121 ccattatcaa gtcttcaaac acagggccgt atataattgc gtttccatgc tcatcagctt
    12181 tttgtacatg cagcacagcc cagtctggtt ttattgccgg cactgttaat atttggttat
    12241 tgccgttatc tgcatatggg ttatcaactg atttaaattt atctacaaga tagctttcct
    12301 taaggtcagc aatcggcata aatggaacgc caaatgccgc ggccctgagg ctggatatta
    12361 cagtataaca agagttttcg ttaaacttga cctgctcact ctccactgat tttctaaaat
    12421 ttggagccag gccaaactct gcctcaaagc ttacaaaccc tgccgtgatt tcatctgtac
    12481 atccggcaag acacagtaga tcaaaatcat agtttgcagc ggttttcatg agttttaggt
    12541 tctttttccc ctgccttgca agctctctta caaatgcaga cggggtgcgc tgtagcagta
    12601 ggcctccaat ggcaagtctg tcaccatctt ttactaattt tgccgattct tccaagctct
    12661 taatcttgtt catgttattt atattcaata ctaaaaccca agtttaacag caattaatct
    12721 acatgtacat ctaaagggtt atgcacctaa tacgttaaat ctaaaaataa atattgatta
    12781 agatattaga ctcagtatta taagcaaaat ttatttttct cagggcttct ttgttatatt
    12841 aatgtctgca aaaattgaac ctttaaagat taaagactgt gaccttcttg ctattgctac
    12901 agggcaaaga gcgcagaatc taaaagagct tagaaataat cttcttacct gtcatcctgg
    12961 ttgtatttac aaccattttt ggggagtgct tgtaagaccc ggatttaccg atccggagtt
    13021 taataacgat tttgcccagt gggcaagcca cagtattcac gatataaaac tcgcagaaac
    13081 ccttggggtt ttggacccaa ctgattttaa gtatatagaa gacctgagat ctcagttaat
    13141 agagataatt gaacagaggc tcgacgagag tgaatacctc acatggtcac actctgacag
    13201 gcagtttaac ttcgtaaggg cagagattgt ggttttcgat accaagcaaa ctattaaaaa
    13261 accacagcat ttaccacgag tagtttcaaa tttctctgtg aacagcatat tttatcattt
    13321 tattgatgca agaaggaggt ctgcacaggg cgaagatgat attagagcct ggttgttaac
    13381 aatagacgga gagtataagg gcctatgcga taagcttgcc caacttgatc cttattttgt
    13441 cacactaaca gagcttagaa atcatattac tcaaatactt aaaacgtatt ttaagaagat
    13501 aaattaatgg gaacactact tgattcatat gcagaagttg ttggtaaaaa tgttattgat
    13561 cacttaaggc agctctcaga gccgcttaag accaagaggg ttattcatgt aaattctaca
    13621 agatatggtg gtggagttgc agaaattctt catcaactta taccacttat gaaagagctt
    13681 gatatagata caagctggga agtaattgag ggaaatgaaa aattctataa atgtactaaa
    13741 agttttcata atgcccttca gggagatgcc gtagatatcc cagaagtact tttaaaaaat
    13801 tttgaaaaga caaacaggca aaatgctaag cagttgcaga gcactttaaa agacgcggat
    13861 atagtattta tacatgatcc gcagccggca ccccttttga actttattcc acaacgcaca
    13921 ggcaagtggg tttggcgttg tcatatcgat gccagcaggc catatagacc ggtttggaaa
    13981 tatttaagac agtttgtatc gggttatgat gccagcatct tttctatagc ggattttgca
    14041 cagccccttc ctcatactca gtatctcatt ccaccaagca ttgatccgct tagtgataaa
    14101 aacagggaac ttgatgatcc tcagatcaat gcagttgcaa aaaaatttaa aattgaccgt
    14161 actagaccca taatgcttca ggtctcaagg tttgacaggt ttaaagaccc tgttggggtc
    14221 attaaagcct ataaacacac taagtcgttt ttgccaaatg tgcagcttat tcttgcagga
    14281 ggtacagcgg atgatgatcc cgaaggagca gaggttttgg cagaggttag gcaagaagcg
    14341 ggtaatgata aggatataca tatactactt ttaccgcctg atgcacatat aataataaat
    14401 gccctacaga ggattgcaga cataatactg caaaaatctg taaaggaagg ctttggcctt
    14461 acagttactg aatcgctttg gaaaggaact cctgtaattg gtggtgatac tggtggaatc
    14521 agactacagg taattgatca ccatacgggc ttttgtgtca acagccccga gggagcagca
    14581 cttaggataa gataccttct acacaaccct gaaaagatta aagaaatggg cagtaaagct
    14641 aaagagtttg tgagagagaa ctttctgatc acaagacacc tgcaggaaca tcttactttg
    14701 atggttaagc ttctctataa caactctagt gacagaatcg agatttaatt agagattagc
    14761 tctacctttc ttccattatt gtattaaaat ctattaatta gaaaaacagg agggctttat
    14821 aaattgttta aaaaaccctg gggcgggaga tttaaaaagc agaccgataa gcttatggag
    14881 aaattctctg cttctattga ttatgacaga aatttgtatg cagaagatat tgaggggagt
    14941 attgcccatg cgaagatgct atctgctacc gggataatta agaaaacaga agcagacaag
    15001 atcataaggg gtcttggctc aattaaaaaa gagatagaga gcggcaagtt tccattcagg
    15061 gatgaatttg aagatataca ccttaatata gaaaaaaggc ttattgaaaa aatcggagca
    15121 gttggcggca aacttcacac tgcaaggagt cgcaacgatc aggtgcttac agatgaaaaa
    15181 ctgtacatca gaaaacagat atcagagata aaaagccggc ttaaaaagtt tgtgggaaat
    15241 atagttaagc ttgccgagaa gaattcagat gttgttatac ctttctatac tcaccttcag
    15301 cgtgctcagc ctgtatacct gtctcattac ctacttagtt attttgaaat ggttaacagg
    15361 gataagcaaa ggcttgatga ctgcctagag aggctaaatg tgctgccgtt aggagcggga
    15421 gcgggagcag gcacttcttt tcccattgat agaaagctgg tggccaggct gctggatttt
    15481 ccagaagtat ccaagaacag tatagataca gttagcgaca gggattttat tatagaattt
    15541 ctgagtgaca gttctattct tatgatgcat ctatcccgga tgtgtgagga ttttattata
    15601 tggtcctcta aagaatttga ttacattgac cttggagatg attttactac aggttcaagc
    15661 attatgccgc aaaagaaaaa ccctgatgca tgcgaacttg taaggggaaa gataggaagg
    15721 gtgtatggaa acctgttttc acttatgaca ataatgaaag gacttccgct ttcttataac
    15781 agggacatgc aggaggacaa ggaacagtta tttgatacag tggatacggt gaagtcatca
    15841 gtcgagctta tatctgaaat gatttcaggt ataaaattta aaaaggacaa tatagcgggt
    15901 tctttaaaag gcggatttat gactgctacg gatattgcag attaccttgc aagaaagggt
    15961 atgccattta gggatgctca tgaggtcacc ggaaatatag tttcatatgc agagaaaaaa
    16021 gaatcagagc ttgaagacct gacattggat gaatataaga agttttccaa gcttattgat
    16081 aaagacatat tctcttgtat tacaatagag ggttcagcaa acagcaggac ttcatatggc
    16141 ggtactgcaa agaaaaatgt ggacaagatg ttaagggaag ctaaaaaaga agtagccaaa
    16201 tggtaaaaaa aatatctatt ctggttttag cccttctttt ggctttggtt gtaattgaaa
    16261 aagtcgtgct acgaaaaaag aatacccctt atgagttttg gcgcttcaat ggtgtgtata
    16321 agctggatga tgatcttata tacagccata aaataaattc caaaagaagc tggagattta
    16381 aagacaacga tagaactatc actgaagtgg tgagtataaa taatttagga cttagagatc
    16441 gtgaattcaa taagaatgag gagttcgata aaagaatatt gatactgggc gactcaatga
    16501 catttgggca cggcgtta
//
LOCUS       DBNGSV010000351         1914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_756636, whole genome shotgun sequence.
ACCESSION   DBNGSV010000351 DBNGSV010000000
VERSION     DBNGSV010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_756636"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="AC159U_12550"
     CDS             complement(<1..346)
                     /locus_tag="AC159U_12550"
                     /inference="COORDINATES: protein motif:HMM:NF040455.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase scaffold domain-containing
                     protein"
                     /protein_id="HIR3337925.1"
                     /translation="MTRQSLNYLFGATILFLWSAVIFSLNITCAGPTHTKGFSINEGI
                     EKSVEQLKKFKALEYKGQSISAVNVIPAFYERRNYSAAWDSKNINDLLYVINEIYKDG
                     LNPSDYHLDLISG"
     gene            complement(359..1084)
                     /locus_tag="AC159U_12555"
     CDS             complement(359..1084)
                     /locus_tag="AC159U_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HIR3337926.1"
                     /translation="MNINTLMLYGNKTKSIKHMYMQYFGNYEIEIDVDRLYSQIGLEP
                     ILDKDIFEYSLTGYTNLIKNNLIKKKSLLTIIDYTKPSTEKRLYVIDLYDKKLLFNSL
                     VAHGKNTGVKYPRYFSNEVGSLQSSLGFFITLGTYFGQNGYSLKLRGVDDDFNDKAED
                     RYIVMHGADYVSEGFIKEHGRIGRSWGCPAVPNEITSSLINIIKEGSPVFIYHNDTKY
                     LNSSKYLNTKTASITNRKRHSLL"
     gene            complement(1348..1728)
                     /locus_tag="AC159U_12560"
     CDS             complement(1348..1728)
                     /locus_tag="AC159U_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337927.1"
                     /translation="MRVLLTVLILLLFVQSVNAEGLKSEAETRAISDKIMQHFIKEDF
                     KTGLDLAKKHWPLPEVEIEGLGVTIKTQWATVRNRFGNPTGTEFVRQQKIGKSFIRYY
                     YLHKFENHALFWRFTFYKPIICGR"
BASE COUNT          628 a          341 c          283 g          662 t
ORIGIN      
        1 gtcctgatat caaatccaga tgataatcag atggatttaa gccgtcttta taaatttcat
       61 ttatgacata taacaggtca ttgatatttt tgctatccca ggctgcagag taattccttc
      121 tctcataaaa cgcgggtatt acatttacgg ctgatatgga ttgtcctttg tattctagcg
      181 ctttaaattt ctttaactgt tctacgctct tctcaatgcc ctcatttata gaaaaaccct
      241 tggtatgtgt cggacccgca caagttatat tcaatgaaaa aatgacagca gaccataaaa
      301 ataaaatagt tgctccgaat agatagttta aagattgcct tgtcatttgt atgacggttt
      361 agagaagtga atgtcttttt ctattagtta tggaggcggt tttagtattt aagtatttag
      421 agctattcaa atactttgta tcgttatgat atataaatac agggctaccc tctttaataa
      481 tattaattaa agacgatgtt atttcattag gtacagcagg acatccccag cttcttccaa
      541 ttcttccatg ttcttttata aatccctcag atacgtaatc tgccccatgc ataacaatat
      601 atctatcctc ggctttatca ttaaaatcat catccacgcc ccttagcttt aaagagtatc
      661 cattttggcc gaaataagtg cctagtgtaa taaaaaatcc caagctgctc tgtagtgacc
      721 caacctcatt agagaagtac cgagggtatt ttacaccggt attcttacca tgagcaacga
      781 gtgaattaaa tagcaatttc ttgtcatata agtctatcac ataaagcctt ttttcagtag
      841 aaggttttgt atagtctata attgttagta gtgatttctt cttaattaaa ttatttttta
      901 ttaggtttgt atagccggtc aatgaatatt cgaatatatc tttatccaaa ataggttcga
      961 gtcctatctg agaataaagt ctatcaacat caatttctat ctcataatta ccaaaatact
     1021 gcatgtacat atgctttata gatttggtct tattcccata taacatgagt gtattaatat
     1081 tcatataaaa tgaaatagaa ataagaatta ggaaagatat aaatagggtt gcaatcttaa
     1141 accagcgcat aacttttcag acacatcaag atatgcacta ttgatcaata gtcaatggtt
     1201 tttactatat ttttgaaaat acagacaaaa ctgaccatta ttaaaatcag ctccgagtct
     1261 taatatttca aatagattga atgttgaaca ttacagttat tgcttactca aacagaaaat
     1321 ctagatcgtc tttataagta ataccgttta tcttccacat attattggct tgtagaaggt
     1381 aaatctccaa aaaagtgcat gattctcaaa tttgtgtaga taataatatc taataaatga
     1441 ctttcctatt ttttgctgcc ttacaaactc tgtacccgta ggatttccaa acctgtttct
     1501 tacagttgcc cattgggttt taattgttac accaagacct tctatctcta cttcaggcag
     1561 tggccaatgt ttttttgcta aatcaagacc tgttttgaaa tcttctttta taaaatgctg
     1621 cattatctta tcagagatag ccctggtttc ggcttcactc ttaagcccct cagcatttac
     1681 agactgtaca aaaagtagta atattaaaac cgttagtaat actctcataa tcgtcctcct
     1741 tgcttgattt ataaacaact tgcaattcta taaaatattt caccgacaat aaatttagga
     1801 gctcgcatag gacagcaaag tatactaagc tgaaactatg caaaagaagt tacttaagaa
     1861 tacaaaacta tcaaatagta agataagcaa gctaatacaa atgtttgtat tgga
//
LOCUS       DBNGSV010000352         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1103426, whole genome shotgun sequence.
ACCESSION   DBNGSV010000352 DBNGSV010000000
VERSION     DBNGSV010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1103426"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2609)
                     /locus_tag="AC159U_12565"
     CDS             complement(<1..>2609)
                     /locus_tag="AC159U_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337928.1"
                     /translation="ITDGKDTCSGDVSTVTSGSPTYGYCSQGDTTTTGACDLVDVDGN
                     GSFDNNVDGEIAAGDIGTRNHPASVGNSNRRSAIQAVNTLRTHFVRTPAVTKNGGSAE
                     KEILSFVICLGADNITDIRTCNAMAVAGGTHTEGVIQHDSPAGQVGKVEPNTTVIASD
                     AGLKALATAKSLTSTPADAWMDGCRLGDEDEGSGVCTIDSNNLFDNAFFASGSTALDN
                     TTLGTSFAFFADNPTELKEALEAISFFIGGFGTTGTAPQAPASSTTVFLRDRIYRALL
                     KPNESEPFWQGRLALYGFVDDPDNPGGKVIIRKPDGDEDLNDGSAVDSLNIFNDDGTL
                     DKTIATSYFWEAGKKLAERDLSGSSNLDTNDFRDIFTIKFSPDPSDSEFDGSVVDTRN
                     MSIRYVGERVRFSEQISPTVVTTLSPEHFSISDADVDPDGDNAIPAACESSCDTTVVC
                     SDITTAGCKTCVKNCLRDRVVKFFSGETGITPIADNLGSPGVGTTCTDDGNSNNSEFG
                     CDCPDPGAGITGSDSQCSVRLGDMFHSAPVIVSSPNPFFFDIGFGRFVNKFLRRTSSL
                     YIGSNSGAIHAFHAGELVEGTVLPSKNPFTLEQESVPFFDEGTGREMFAFIPGYFMPD
                     TRSPELTDPTTPLSTYFNSESPAPSPLYDTPEYRFGDLKDTALYGKTQRSYVDGTFLI
                     ADVWIDGYKNGIKNDPNVCKSKDSQSDTSTDVDLNGVIDPCGKEWHTVMLAGNRNGGG
                     FYTALDVTGPKCSGSCSTDSNAIDNAASIKLGSTAEIADAPQYPRHLWTLYDKHFGNT
                     WSDPTIGRVRLKTDADGTPLITDRWIMFVGGGMDPENIFPNKDTDGDGTLDGADFDDN
                     VNYVGNGFYAVDIA"
BASE COUNT          690 a          645 c          527 g          747 t
ORIGIN      
        1 gctatatcaa cggcatagaa gccatttcca acataattaa cattatcatc aaaatcagcc
       61 ccgtctaacg taccatcacc gtcggtatct ttattcggga atatattttc ggggtccata
      121 cctcctccta caaacattat ccagcggtct gttataagag gcgtcccatc agcatctgtt
      181 ttaagcctta ctcgtcctat cgtaggatct gaccatgtat ttccaaagtg tttgtcatag
      241 agagtccaca gatgccttgg atactgggga gcatcggcaa tttcagctgt gctgcctaac
      301 tttatggaag cagcattatc tattgcgttt gagtcggtac tgcatgagcc tgagcactta
      361 ggtcctgtca catcaagtgc agtatagaaa cctccaccat ttctgtttcc ggcaagcata
      421 acagtgtgcc attccttgcc gcacggatct attactccgt tcaggtcaac atctgtagaa
      481 gtatcggact gagaatcctt acttttgcaa acgttcgggt cattctttat tccattttta
      541 tatccatcaa tccatacatc ggcaattaag aatgtgccgt caacataaga cctctgagtt
      601 ttaccatata gagcggtgtc ctttaagtct ccaaatctat actccggcgt gtcatataaa
      661 ggcgatggtg cagggctctc gctgttaaaa taagtactaa gcggtgtcgt aggatctgta
      721 agttcgggtg agcgggtatc tggcataaaa taaccaggga taaaggcaaa catctccctt
      781 cctgtaccct cgtcaaaaaa cggcaccgac tcctgctcca aggtaaaagg atttttagat
      841 ggcagaaccg tgccttcaac aagctcgccc gcatgaaaag cgtgaatagc gccactgttt
      901 gagccaatat agagtgaaga tgtcctccta aggaacttgt ttacaaatct tccaaatcca
      961 atatcgaaga aaaacggatt tggagatgaa actatcacag gggcagaatg aaacatatca
     1021 ccaagcctta cagaacattg tgaatcactg cctgtgatcc ccgcacccgg gtcaggacag
     1081 tcgcacccga attcactatt attggaatta ccatcatcag tacatgtggt tccgacacct
     1141 gggcttccca aattatcagc tataggggta atccctgttt ctccactgaa aaatttaact
     1201 acacggtcgc gaaggcagtt tttcacacaa gttttacagc cagctgtagt tatatccgaa
     1261 caaactaccg tagtgtcaca gctggattca caggcagctg gaatagcatt atctccatca
     1321 gggtcaacat ccgcatcgct tatagaaaaa tgttcaggac taagagttgt tacaactgtt
     1381 ggtgatatct gttctgaaaa tctcaccctc tcacccacgt accttatact catgtttctt
     1441 gtatcgacta cactaccgtc aaattccgaa tctgaagggt cgggtgaaaa tttaattgtg
     1501 aaaatatccc tgaaatcatt tgtatccaga ttacttgatc ccgacaaatc tctttcagca
     1561 agctttttgc cagcctccca gaaataagat gtagctatgg tcttatctaa cgtgccgtca
     1621 tcattaaaaa tattaagact gtccactgcc gagccgtcat ttaagtcttc atctccgtcg
     1681 ggttttctta tgataacctt gcctccggga ttgtccgggt catctacaaa cccgtaaagc
     1741 gcaagacgtc cctgccagaa aggctcggat tcattaggct ttaaaagtgc acgatatatc
     1801 ctgtccctta aaaatactgt tgtagaagat gcaggggcct gtggtgcagt accagtggtt
     1861 ccaaatcctc cgataaaaaa tgagatagct tccagagctt ccttaagttc tgttgggtta
     1921 tccgcaaaga acgcgaagct tgttccaagt gttgtattat ccagcgctgt tgagccagaa
     1981 gcaaaaaatg cattatcgaa cagattattg ctgtctattg tacatacacc gcttccttcg
     2041 tcttcatcac ccagtctgca gccgtccatc caagcatctg caggagtcga agttaagctt
     2101 tttgctgtcg caagcgcttt caatccggca tcgcttgcta ttactgttgt gttcggttct
     2161 actttaccaa cctgtccggc ggggctgtca tgttgtatta ctccttctgt atgggttccg
     2221 cccgctactg ccatggcatt acaggttctt atatcagtga tgttgtcggc tcctagacat
     2281 attacaaatg acaatatttc tttttctgcc gaaccgccat tttttgttac agcaggagtc
     2341 ctgacaaaat gggtccgcag tgtgtttacc gcctgtatgg cgctccttct gtttgagtta
     2401 ccaactgaag ccggatggtt tcgcgtaccg atatcacctg cagcaatctc tccatcaaca
     2461 ttattatcga aggacccgtt tccatcaaca tcaacaagat cacatgcccc cgtggtggtt
     2521 gtgtcaccct gagaacagta cccataagtc ggagaaccgc ttgtaactgt agatacgtcc
     2581 cccgagcatg tatcctttcc atctgttat
//
LOCUS       DBNGSV010000353        24768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1935073, whole genome shotgun sequence.
ACCESSION   DBNGSV010000353 DBNGSV010000000
VERSION     DBNGSV010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 24768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24768
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1935073"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC159U_12570"
     CDS             complement(<1..219)
                     /locus_tag="AC159U_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR3337929.1"
                     /translation="MKQSQILELIERIGNLLRAEQRIIGSAQQLQPVHIQVLEYLNRC
                     NKYSNTPAGVVKYFGITKGTVSQSINVLE"
     gene            323..811
                     /locus_tag="AC159U_12575"
     CDS             323..811
                     /locus_tag="AC159U_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3337930.1"
                     /translation="MKKSIVFGVFVLVVGAVGCMKTVGQSSQIAYPDGYRSWTHVKTM
                     VIEPGHPLENPFQGIHHIYGNEQAIEGYRSGTFQDGAVIVFDLLNYNQADKTIQESDR
                     KLIGIMQKDSAAYASTGGWGFEGFAGDSKTERLVADGGQSCFSCHQSQKESDYVFSKW
                     RD"
     gene            complement(867..2066)
                     /locus_tag="AC159U_12580"
     CDS             complement(867..2066)
                     /locus_tag="AC159U_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337931.1"
                     /translation="MKITKLYKLLLFSLLCMFISPKISNAQAIVVQFLGTSDVSVQMI
                     DTDGDGENDVESNCYENDLVDPTSGEVVGTGLDCLRDIVANEDGSVTLISNIFFDFDG
                     FGLISSEGTTTVQPLLTQSALDAGLTNGTGSFSDEDTIMFGTGVFEDATGNVRLSGLV
                     DLSSFPNEAFFNCVFEIAVDGDPAPNITGSGDEAIVVKFLGTDSGQDTMIDTDGDDVN
                     DVESTCFENDLVDPNTGNVVGHGIDCLRDITPVEDTNGLTLTSNIFFTFDSGGLNGLI
                     SSEGTTTVQPLVTDSALDAGLTHQTQSFRDNDTIKFGTGDFAGATGNARLSGLVDLTT
                     FPDDTFFNCMFIIKANRIVGVKSGSGCALASNNDYAQSGAINYLMMLLVPLLIIGIRK
                     RYFASNS"
     gene            complement(2145..2888)
                     /locus_tag="AC159U_12585"
     CDS             complement(2145..2888)
                     /locus_tag="AC159U_12585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3337932.1"
                     /translation="MKVLYLVFSLLLICISYSKPAVSELPDPGMEIDDRTALVVTDPQ
                     NDFLSPKGVTWGVVGESVTENKTVPNIESLLKIAKATNIPVFVSPHYYYPTDKGWMFE
                     GALEVLMHKINMFDRAGPLSLEGFEGSGADFLEPYKPYIEDGKTVVTSPHKVYGPEAN
                     DLVLQLRKRKIDKVILAGMSANLCVEAHMRELLEQGFEVTVVTDGTAAAKVPEGDGYK
                     AAMVNFRFMANDLWTTKEANEKMKSFAKK"
     gene            3071..5017
                     /locus_tag="AC159U_12590"
     CDS             3071..5017
                     /locus_tag="AC159U_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3337933.1"
                     /translation="MNVTKKNIIEYTDFFKSAIDSEPDGILIIDTDGNFLYYNEKFLD
                     VWKITSDKIESKKKSDIFEYFGTLVKHSEDYNEKFNELVNNRLKDTFEILEFKDGRIF
                     EKITTPQKLNDQVIGRVCIFKDITRTRKSEKTLQETLLKLSRKNRNEEIINNILKDIH
                     KTIDLKEVLRNASNSLFENLDSINGVEIYLAEQDNAVLSGYAGLPNWYLEQFEIIKYP
                     NGIVWNIISTGKAKYCEDLEKEIQASPEVLKLGTKSCLSLPIKQSGKTIGCINLHAME
                     MDAFSEDELRLLEIVSKEIEVSVNNAKQAQELKNALEELEILKDQLHHENIYLKEEIR
                     LNDNFEEIIGEDIELKKILNKVEIVAPTESAVLILGETGTGKELIARAIHNLSSRNTR
                     SFVKVNCGAIAPTLIESELFGHEKGAFTGAVKEKIGRFEIADGGTIFLDEISELPVDL
                     QVKLLRILQEGEYERVGSSNTQRVNVRVIAATNRNLELSIKQNKFRADLYYRLNVVPI
                     LVLPLRERKSDIPLLVNYFITQYSKKLGKNIRRISVKSHDKLLRYNWPGNIRELQNVL
                     ERNIVLSKGSTLHIEDQFIDNIELQDNVNLMRLENIERNHIVKILEKTNWKVHGEQGA
                     ASILDINPSTLRARMRKLGVTRPQ"
     gene            complement(5067..5669)
                     /locus_tag="AC159U_12595"
     CDS             complement(5067..5669)
                     /locus_tag="AC159U_12595"
                     /inference="COORDINATES: protein motif:HMM:NF017853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HIR3337934.1"
                     /translation="MSALLETLGQVLNSGAVGQISRQIGSDEGTTGNALSAALPVLLG
                     ALSRNATRGDGAEKLLGALSRDHDGGILDNLDVFLQNPESGSGSGILRHMLGGRQPRV
                     ESSLSRSTGLDAGSIGKLLVTLAPIIMGALGKSRRQNNMNSRDLSDMLNTERASFERS
                     QPQASNPLNQLLDVDGDGDVDMADLMKGGLSSVLGNMLGK"
     gene            5847..6470
                     /gene="amrA"
                     /locus_tag="AC159U_12600"
     CDS             5847..6470
                     /gene="amrA"
                     /locus_tag="AC159U_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR3337935.1"
                     /translation="MGNTSEFQKLLNEHLMQTAGVDTEKQLSDNDKSILLKIARNTLK
                     TFLETREIPEVVSDQASLLQPKATFVTLRKKDTNELRGCKGEIFPSKPLAEAVQNTAI
                     SSATSDPRFCEVSAGELPNIKIEISVLKPIKPIKPEEVVIGRHGLVISKGSRTGLLLP
                     HVPIVYNLDKETYLKEICFKAGLKENALEDENTDLYGFEAEVFEESV"
     gene            complement(6472..7362)
                     /locus_tag="AC159U_12605"
     CDS             complement(6472..7362)
                     /locus_tag="AC159U_12605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3337936.1"
                     /translation="MKIGIAVPNFGKYATKDNITDIALAAEEYGFDSIWVSDHIVIPE
                     SHNVFGNNFYDPVSTLSYLASITTTITLGTSILVLPYRNPIVTAKAVTTIDQLSEGRL
                     ILGVGAGWLEDEFEALGANYENRWSKTLEYIDILQSLWYCEKKDYKGKYFSFSNISFK
                     PRTYNNREIPIWLGGNSIKSINMAFDKCSGWHAVRLNADELMEKRKYIERKFEKTVTR
                     RETFSISTRTNLQITDNSTINANVDEPLRGNKGKIIKGLSNYKSAGADHMIIYILSSN
                     ISNFINTMKTFANVIRPQID"
     gene            7447..7977
                     /gene="cyaB"
                     /locus_tag="AC159U_12610"
     CDS             7447..7977
                     /gene="cyaB"
                     /locus_tag="AC159U_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR3337937.1"
                     /translation="MKSNIEIKARIQNLHEVQKRINEYYGSDYTVLNQKDIYYGCNTG
                     RLKLRIFSEDEAELISYRRDNIKGPKKSDYSIYKTNNPGQLNKALESSLGIIGVVEKR
                     RLVYITGQTRIHLDDVAGLGNYLELEVVLKSGQSADEGLVIAESIIKELEVKKEKLIE
                     GSYIDLLLNRNETQKI"
     gene            7958..8245
                     /locus_tag="AC159U_12615"
     CDS             7958..8245
                     /locus_tag="AC159U_12615"
                     /inference="COORDINATES: protein motif:HMM:NF021402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HIR3337938.1"
                     /translation="MKHRKSKIKRQHSLIKGLQDYLEKYISSLEYVTGVIPGEIKVSK
                     ATGENLHVKYKYETVSGAKLIAKSGTSIQEVFVITKDPQKIEKLFAELNPS"
     gene            complement(8238..11675)
                     /gene="dnaE"
                     /locus_tag="AC159U_12620"
     CDS             complement(8238..11675)
                     /gene="dnaE"
                     /locus_tag="AC159U_12620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3337939.1"
                     /translation="MSDQFIHLHLHSQYSLLDGAIKFNDLFTKAKEYEMDSIAITDHG
                     NLFGAYEFHQKAKEFGIKPIIGCEIYITPTLKVDHPSQARNHHLILLATNTAGYKNLS
                     NLVTKAYFDGFYRRPRIDHELLELHSEGIISLSGCLNGELCTSILEDDMNKAMAVASK
                     YKEMFGDRYYLEVQANDLQEQELVNEKIKQIGKSLKIPIVATNDCHFLNKHDSKPHDA
                     LLCIQTGSMLQDENRFKFKGEEYYFKSAEDMVSSLGKYEDEIYKTAEIAERCNFEFET
                     TGYKLPVFKIDNGKSLEEYLLELTKDNLNKTIRDKSLDGDQKKIYQERIDREILTINE
                     MGFAGYFLVVADFINYAKSKGIPVGPGRGSAAGSLVAYALGITEVDPIPYNLIFERFL
                     NPERVSMPDIDIDFCGEGRDEVIRYVTDKYGADKVAQIGTFGTMSAKAVVKDVGRVMG
                     IPYADVDRVSKLIPSFRGRVFSIEEAIKNVPELKDLISSSEDLTELIELAKPLENMVR
                     HSSTHAAGVVISNEPLSDYVPLYKGAKDEIVTQFDMNSIESLGYVKFDFLGLKTLTVI
                     NKAIQLIKQSDDPEAKNFNISNIPLDDPNVYKLLSRGFTRGIFQIESPGMKEVLSRLQ
                     PTDFEDIIALLALYRPGPLDSGMVDEFIQRKHGKKKVDYPLPDLQEILKDTYGLFVYQ
                     EQIMNTASIVAEYSLGEADLLRRAMGKKKPAEMKAQRKRFLEGSKNRSIAPKKAKEIF
                     DNMEKFAEYSFNKSHSTAYAMITYQTAYLKTYYAPEFMAALMTVEAGNTDKIIANIAE
                     CRDMGIEILPPDVNESLSGFTSVNGKIRFGLSAIKNIGESAIDSIIKEREENGRFSTI
                     FQFCKNMDSKKINKKSLESLVKSGAFDSINKNRGQLYESIEMLLNFIALNTKLNSQNQ
                     NSLFPLEDSISLPELADTENWDEKNILRCELEVLGFFVTNHPMEKYAHDLARLVKIKD
                     SESIKEIPDGKEASIAGVVRTLNVRNTKSGSGIFGNLVLEDLKGSVEAVVFNDLLRKS
                     LHILEDRTEPIILKGIVEHTEERTKLRSLDISSLKELKSMSTLNVQLEQQKANSEYYS
                     KLQDAFLKFPGDSKINLNIETEDGKAVIEVGEYRVSIDDPLIDHLETMLGKGTVSIS"
     gene            11846..13210
                     /gene="ftsY"
                     /locus_tag="AC159U_12625"
     CDS             11846..13210
                     /gene="ftsY"
                     /locus_tag="AC159U_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3337940.1"
                     /translation="MESIINNLSSTLNMNPQDLTFILICAVIFLVTLILAIFVKDEVP
                     EVSEPEEDSSQAAEDAVVAEQLQPADLEEIQGKVEQQTEVSEEVVSEPAPEFVAKTEP
                     RESEAAPKVEVKVEPVVPPQIPQEIVEEVTEEQPDEQIEEAPKESFFSRLRKGLSKTR
                     TGFIDKLDAIISRTEIDDEIWKDFEETLVMADLGVTTTMKLREDVEREIESNSITDPH
                     EIKNILKAKILAILKESEGGAVSPQTKPFVMMVAGVNGVGKTTTIGKLSNKFKNEDKK
                     VMVAAADTFRAAAVDQLEVWAKRVGTDFIRGQSGADPSAVAFDAVKAAVSRGADILII
                     DTAGRLHTKDNLMDELKKMRKVINKELEGAPHETILVLDATTGQNAIQQAKLFNEAID
                     LTGIALTKLDGTAKGGVIISIANELSIPVKYIGIGERLEDLREFNAEQFVEALFGDEE
                     HTIH"
     gene            13219..14334
                     /gene="ribD"
                     /locus_tag="AC159U_12630"
     CDS             13219..14334
                     /gene="ribD"
                     /locus_tag="AC159U_12630"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR3337941.1"
                     /translation="MSNDIDAKYMALAIKLAKKASGMTSPNPVVGSVIVKNGEIIGKG
                     YHKKAGLPHAEIEAINNARKQGKKIAGSTLYVTLEPCCHKNKKTPPCLDAVLEEKFSR
                     VVIGSLDRNPQVSGKSVKALKRKGVEVSVGVLEDQCLKINEAFFKHISKKIPFVTLKL
                     ASTLDGKIATFSGDSKWIGSEGQRKQGHILRSLSDAVLVGINTVKTDDPMLNVRLEKK
                     RVDKQPIAVVLDTNLEIPLSAKLLTERSGTMIFCSKNINKVKKMKRLKDLGFVVVKTG
                     LDKNGFVNTKEVLKKLGSRGIMSVLVEGGSKVASSFIRYGLVDKAIFFYAPKIVGADG
                     KSMIGELGINEIKDSFELSNVNISSIGNHVMVEGYLN"
     gene            14353..14997
                     /gene="nadD"
                     /locus_tag="AC159U_12635"
     CDS             14353..14997
                     /gene="nadD"
                     /locus_tag="AC159U_12635"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3337942.1"
                     /translation="MKQGLFGGTFNPIHSGHVKAAEQILEILDIDRICFIPSNIPPHK
                     DQKELATPEQRYEMVKLATHGNDRFSISDFEISSNKESYTIDTLKYFKDKLPEDDLYF
                     IVGHDIFNQIDIWKEYKKLFELSNFIVISRPGYFDDDEDLPLAIKDDFRYDKTETGNT
                     YYKHKNSNLIIKTQIEGIDISSSEIRELIKNNKPIHELVPPKVEQYIFDNNLYA"
     gene            14990..15373
                     /gene="rsfS"
                     /locus_tag="AC159U_12640"
     CDS             14990..15373
                     /gene="rsfS"
                     /locus_tag="AC159U_12640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3337943.1"
                     /translation="MPKNGSLTPKKKALALAEAAYDKKAENIVLLHMTKLSDFADYFV
                     ICSADSDRGVKTIVENIEKKCKELGSKPLGIEGLKECRWVLIDTGDTLVHVFHGPVRA
                     EFDIESLWNEAPRVKLPFEQEQVAS"
     gene            complement(15646..17442)
                     /gene="lepA"
                     /locus_tag="AC159U_12645"
     CDS             complement(15646..17442)
                     /gene="lepA"
                     /locus_tag="AC159U_12645"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3337944.1"
                     /translation="MDPKFIRNFCIIAHVDHGKSTLADRILEYTGTVEKRDMEEQFLD
                     KMDLEKERGITIKAQSVKINYKSEDGQLYEFNLIDTPGHVDFSYEVSRSLEACEGALL
                     VVDASQGVEAQTVANAYLAVDIGLELIPVINKIDLPSADPERVKREIEDIVGIDATDA
                     TLASAKTGIGIKEILESIIHLIPPPQDNRGKPLKALIFDSWFDSYQGVVMLVRVFEGA
                     INVGENIMFMSTGKSFDVKTLGVFSPTAIALKTLGTGEVGLVTASIKDIEEAKVGDTI
                     TSLENPTDKPIPGFREVQPMVFSGLYPIDTNDYEKLKDALLKLGLNDASLTYEPETSV
                     ALGFGFRCGFLGLLHMEIIKERLEREFDLNLITTAPTVVYRVIDKTGKMTEVENPSKL
                     PTPDKFEIIEEPYVKVTLHTPNQFLGAIFDLCRSKRGIQKNLSYKTEDRVLVEYELPL
                     SEIVYDFYDTLKSISKGYASMDYEPIGYRASDLIKMEILINGDPVDALSVITHKEKAY
                     ERGKALIEKLRSLIPRQLYDVAIQAAIGNRIISRESVKALRKDVTAKCYGGDVSRKRK
                     LLEKQKEGKKRMKQVGSIEIPQEAFYAVLKND"
     gene            complement(17496..18635)
                     /locus_tag="AC159U_12650"
     CDS             complement(17496..18635)
                     /locus_tag="AC159U_12650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3337945.1"
                     /translation="MKTYLFTPGPVSVPEEVLLQMAKPIIHHRTEEFESIFAEVREGL
                     KYVYETQKEVYILAASGTGAMEAAVANTLSRGDKVLVVNGGKFGERWGKISKAYGLEV
                     VEETVEWGYSVDPARIEEHLNNDKDIKAVMIQASETSTGVMHPTKEIGQITKKRDDVI
                     LIVDGITGVCVFPLPFDEYGIDVLVGGSQKAFMLPPGLSFAAMSDKAWGFYEKSDLPK
                     FYFDFKSCEKNALKNTTAFTPAVSLIIGLAQVLRNFRAEGLENIHNRHEKLALATREA
                     MKAIGLEGFVKGTPSTALNVTYAPEEIGAKKMISSLSSDFGITLAGGQDHAKGKIFRM
                     SHIGYIDRADTIGVISAIESVLKNLGHEFEFGAGTKVASEILRNM"
     gene            18769..19873
                     /gene="prfB"
                     /locus_tag="AC159U_12655"
     CDS             join(18769..18837,18839..19873)
                     /gene="prfB"
                     /locus_tag="AC159U_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148894222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3337946.1"
                     /translation="MFTENKDKIQDIKTRLKLLGDYLDLPAKKDRLYELDLEVSQADF
                     WDNKESAQKVLKEQSQIKTIVSDWSSLSEELEEAELLSELSAEENDETTGAEVQGKLS
                     ALYKRLESLEFKRILGEPEDPNNAIISINSGAGGTEAQDWTEILLRMYLRYCEMSGFD
                     VQMLDNQPGDDAGVKSCTFLAKGPYAYGYLKGESGVHRLVRISPFDSNKRRHTSFSSV
                     FVTPEIEDDIDIEIEEKDLRVDTYRASGAGGQHVNKTDSAVRITHIPTNIVVSCQNER
                     SQHQNRATCMKILKGRLYELEKQKQQEKLDNIESSKMEIGWGSQIRSYVFHPYRMIKD
                     HRTGFETGNVDPVMDGDLSEFIKNYLLHSSNNN"
     gene            19903..20766
                     /locus_tag="AC159U_12660"
     CDS             19903..20766
                     /locus_tag="AC159U_12660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3337947.1"
                     /translation="MKVGIVGKKDNSEVIKLAKLLIEKFGSQGIEVFLEDELAVLVDH
                     ANSTKAEKIQEKSDVVIVLGGDGTFLGVSRNLSGTDVPVVGINMGNLGFLTEVTREEA
                     LELVDIIAKGTYDIETRDMITARVLRDGKQFCKYDVLNDVVISRGVVSRVVDLSVETN
                     NKQITTVKADGLILSTPTGSTAYSLSAGGPIVFPTLPLMIITPICPHTLTNRPLVVSN
                     ERKIRVRSLSEETELHLTLDGQISFDLIPNDVVEVSKSEKTVKFIKSPFRDYFTILKT
                     KLMWGERYGTL"
     gene            20753..21121
                     /locus_tag="AC159U_12665"
     CDS             20753..21121
                     /locus_tag="AC159U_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337948.1"
                     /translation="MARSEDEQFLHDWVIRKIKEKNGRMYSEVNINPGDEQNYDLEGK
                     YPDVVMVNHGQVVGIIEVDTKETVDESCVDKWKALSELGAKLTLVVPKEEQKKARDLV
                     WKSGLVAKVDIKFFDVHIGV"
     gene            complement(21118..22044)
                     /gene="fmt"
                     /locus_tag="AC159U_12670"
     CDS             complement(21118..22044)
                     /gene="fmt"
                     /locus_tag="AC159U_12670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3337949.1"
                     /translation="MKIIFMGTPEFAVPSLKALTESEDEVIAVVCQPDKPVGRKRELH
                     KPLTKQFAESRNISVLQPAKIRNNEKFLNELKQLAPDLICVAAYGKILPKEVLDLPVH
                     GCINVHSSLLPKYRGAAPINWAIINGESRTGVTTMLMDEGMDTGDILLIEETNITADD
                     TSETLTKRLSEIGAELLIKTIDELKQEGLNPIKQSDSEATLAPILKKEHGEIDWSKSA
                     TEIRNQIRGLLPWPVAYTNLNGKMLKIFSADVSEKSGDPGVVLEASKSILRIACGSGS
                     LDLKELQLEGNKKMEIRSFLAGRKIESGMRLG"
     gene            complement(22062..23360)
                     /gene="rimO"
                     /locus_tag="AC159U_12675"
     CDS             complement(22062..23360)
                     /gene="rimO"
                     /locus_tag="AC159U_12675"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3337950.1"
                     /translation="MKISLTSLGCSKNLVDSELILGSLTNSGHEITTEDKADIIVINT
                     CGFIGDAKKESINTIIDLAEYKKTGSCKKLIVTGCLVERYSQQLKDEFPEVDHFWGTG
                     NLLKINEVLSDTIYDFNKTPFGTIYDPDSPRVMLTDSHTSYVKVSEGCSRTCSFCIIP
                     KMRGIMKSRSVDSIYNEISMLGQNGVKEINLIAQDMTSYGRDIGTSLYTLLKELITID
                     SIEWMRLHYCYPWGVTDELVDLLADDNNLLPYIDMPIQHINDRILKLMDRKTPKKRIL
                     SIVNKLKQSMPDLTLRTTFIVGFPSETEDEFEELLEFVEDTKFQRAGAFRYSHEEGTP
                     SFDIDGQIDEDIKEERLERLLNVQSDISLENNKALIGKTFGYFVEGIEDGNYFGRIST
                     QAPEVDGLTYITTDKKLKKGDFLQVRITEADIYNLHAELV"
     gene            23514..23777
                     /locus_tag="AC159U_12680"
     CDS             23514..23777
                     /locus_tag="AC159U_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337951.1"
                     /translation="MKKLLAVTAIALSMGLSSISFADTNVFGVNVPVKKNQVSDHIKG
                     SYVESDHISFYLNAKDSVDVNSTEHARRFSLEDYGQYVFGVKI"
     gene            complement(23845..>24768)
                     /locus_tag="AC159U_12685"
     CDS             complement(23845..>24768)
                     /locus_tag="AC159U_12685"
                     /inference="COORDINATES: protein motif:HMM:NF019344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3337952.1"
                     /translation="DLPPRPNSILIGPTGCGKTYILELLFGQILKIPYVIIDMTKFTE
                     SGYVGDDVLNILVQLVYASNENLDIAECGVVVLDEFDKLAGSYSNAHFAGQGTTKDVS
                     GYGVQRELLKILEGTDIQIPLDFGFSNRGPRAMMSTRDISFFAVGAFSGIKDMFREDG
                     MGFEQKLAQGADQKYDIAYKISDEQADDISQFHLFGFLPELIARFNRIIPFEPLGRDT
                     LKDILILKVENYKREFIQEGYTLNIDSSLADLVVDESIKRQTGARGLDVLLSKYIEDV
                     AFELFGKGNSGQINIKADRNGKVSHQIKKRA"
BASE COUNT         7573 a         4770 c         4807 g         7618 t
ORIGIN      
        1 ttcaagaaca tttatagatt gtgatactgt tccctttgta ataccaaaat atttaacgac
       61 tcctgcgggt gtgttgctgt atttattgca ccggtttagg tattcaagaa cctgaatgtg
      121 tacaggctga agctgttgtg cagagcctat tatcctctgc tctgctctca gtagatttcc
      181 gattctctcg attagttcca gaatttgcga ttgtttcata tctttataat agtatcgtgg
      241 caaaactatc ttgacaagta gttttatatt tgatatatag tatagagtcg ataccaatat
      301 aatgcttata aggaggcttt taatgaaaaa atctatcgtc tttggtgtat ttgttttagt
      361 cgttggagct gttgggtgta tgaaaaccgt tggacaaagc tcacagattg catatccgga
      421 tggttaccgg agctggacac atgtaaaaac aatggttata gagcctggac atccgcttga
      481 aaatccgttt cagggtatac accatatcta tggcaacgaa caagcaattg agggttatag
      541 atcaggcact tttcaggatg gtgctgttat tgtgtttgat cttttaaatt ataatcaggc
      601 cgataagaca attcaggaat cagatagaaa actaattggt attatgcaaa aagattcagc
      661 cgcatatgca tccacaggag gctggggatt cgaaggattt gcaggggaca gtaaaacaga
      721 gcgccttgtt gcagacggag ggcaaagctg ctttagctgc catcagtctc aaaaagaatc
      781 tgattatgta ttcagtaaat ggagagatta aaaacaagat agtattaaac ccacctcaaa
      841 taatattgag gtgggtttaa atcactctaa gaattacttg caaaatatct ttttctgata
      901 ccaattatca acaacggaac caataacatc attaaataat taatagcacc gctttgtgca
      961 taatcattat ttgatgccag agcacatccg cttccgcttt taacaccaac aatcctgttt
     1021 gccttaataa taaacatgca attgaaaaag gtatcgtctg gaaaagtagt taaatccaca
     1081 agtcccgata gtctggcgtt tccagtagcg cctgcaaaat caccagtccc aaatttgata
     1141 gtatcattat ctctaaagga ctgggtttga tgagttagac ccgcatccaa tgcagaatct
     1201 gtaaccaggg gctgcacagt ggttgtacct tctgaggata tcagaccatt tagccctcca
     1261 ctatcaaatg tgaagaatat gttgcttgta agagtaagac catttgtatc ttcaaccggt
     1321 gttatatctc ttagacagtc aattccatgc cctacaacat taccggtatt tgggtctaca
     1381 agatcatttt caaagcatgt agattccaca tcatttacat catcaccatc ggtgtcgatc
     1441 attgtatcct gtccggagtc tgttcccaga aatttgacta ctatagcctc atcaccgctt
     1501 cctgtgatat ttggggcagg atcgccatcc actgctattt caaaaacaca gttaaaaaat
     1561 gcttcatttg gaaaactgct aaggtcaaca aggccggata aacgtacatt acccgtagca
     1621 tcttcaaata cccctgtgcc aaacattatt gtatcctcat ctgaaaagga accggtgccg
     1681 tttgtaagcc ccgcatccag agcagattga gtcagtaatg gctgaaccgt ggttgtacct
     1741 tcagaagaaa taagcccgaa tccatcgaaa tcaaagaata tattgcttat aagagttaca
     1801 cttccatcct cattagccac tatatctctg aggcaatcaa gaccagtgcc aactacttct
     1861 ccgctggttg gatcaaccag gtcgttttca tagcagttag actccacgtc attttctccg
     1921 tcaccatcgg tgtctatcat ctgtacactt acgtctgatg ttccaagaaa ctggactaca
     1981 atggcttgtg cattagatat tttgggggag atgaacatac acagcaaaga aaataataat
     2041 agtttataaa gttttgtgat tttcatttat ataaaactct cctttaatat atatttacag
     2101 ctaaatcatt tccatagaga ataagcaaat atgtattgcc aggattattt cttagcaaag
     2161 cttttcattt tctcattggc ctcttttgtt gtccaaaggt catttgccat aaatctgaag
     2221 ttgaccatgg ctgccttata accatcaccc tcaggaacct ttgcagcagc tgtgccgtct
     2281 gttacaaccg taacttcaaa accctgttcg agaagttctc tcatatgagc ttccacacaa
     2341 aggtttgcag acatacctgc aagtattacc ttatcaatct ttctcttcct taactgcaat
     2401 acaaggtcat ttgcctcggg tccgtaaacc ttgtgcggac ttgttactac tgtttttcca
     2461 tcttcaatgt agggcttgta aggctcgaga aagtccgctc ccgaaccctc aaatccttcg
     2521 agagataaag gcccggctct gtcaaacata ttgattttat gcatcagaac ttcaagcgct
     2581 ccctcaaaca tccagccttt atccgtagga tagtagtaat gaggggatac aaaaaccgga
     2641 atattggttg ccttggcgat ttttagcagg ctttcaatat taggaactgt cttattttct
     2701 gtaacgcttt cccctactac accccatgta actcctttgg ggctaagaaa atcattttgt
     2761 ggatctgtta ctaccaaagc cgtcctatca tcaatttcca ttcccggatc aggcagctca
     2821 gaaactgctg gttttgaata agatatacat atgagtaata gagaaaatac taaatataat
     2881 actttcatcc tatttacctc cttaataaaa gttaatatac tttatgcatg gggtatgcca
     2941 tatctgcaaa cctgtcttaa gtcactgaat ttatggaata ttttggaaat cgttaggtat
     3001 gaagatgaat aggtattcaa aaatattagt caaatatgac agcagttgta aaatatgacc
     3061 attaaaaata atgaatgtta ccaaaaaaaa tataattgaa tacaccgact tttttaagtc
     3121 ggctatcgat agtgagccag atggaatttt gatcatagat actgatggta attttcttta
     3181 ttacaatgaa aagttcttag atgtctggaa aatcaccagc gataaaatag aaagtaagaa
     3241 aaaatcagat atttttgaat atttcggtac tcttgttaag cactcagaag attacaacga
     3301 gaaatttaat gaactcgtta ataacagatt gaaggatact tttgagatac tggagtttaa
     3361 agatggacga atttttgaaa aaatcacgac tcctcaaaag ttaaatgatc aggtaattgg
     3421 aagagtatgt atttttaagg atataaccag aaccagaaaa tctgagaaaa ctttacagga
     3481 aaccctctta aaattatcaa gaaaaaatag aaatgaagaa ataataaaca acattcttaa
     3541 ggatattcat aagactatag accttaaaga agtattgaga aatgcttcaa attccctctt
     3601 tgaaaattta gatagtataa acggtgtaga aatatatctt gcagaacagg ataatgcggt
     3661 actaagcggc tatgcaggtc ttccaaattg gtatttggag caatttgaaa ttatcaaata
     3721 tccaaatggc atagtctgga acattatttc caccggtaag gctaaatatt gtgaggatct
     3781 agaaaaagaa attcaggcaa gcccagaagt actgaagctc ggcactaaaa gctgtctgtc
     3841 actacctata aaacagagtg gtaagactat tggttgtata aatttacatg cgatggaaat
     3901 ggatgctttt agtgaagacg agttgagact gctcgaaata gtatctaagg aaattgaagt
     3961 atctgttaat aatgcaaaac aggcccagga attaaagaat gcattagaag agttggagat
     4021 attaaaagac caactgcatc atgaaaatat ttacctgaaa gaagagatta gattaaatga
     4081 caattttgaa gaaataatag gtgaagatat tgagcttaag aaaattctta ataaagttga
     4141 aatcgtagct ccgactgaat ctgctgtact tatattgggg gaaaccggaa ctggtaaaga
     4201 actaatagca agggcaattc ataacctaag ctcaagaaat accaggtcat ttgtaaaagt
     4261 taattgtgga gccatagctc ctacgcttat tgaaagtgag ttattcggac atgaaaaagg
     4321 cgcttttacg ggggctgtta aggaaaaaat tggcaggttt gagattgccg atggcgggac
     4381 catattttta gatgaaattt ctgaattacc tgtggacttg caggttaagc ttcttaggat
     4441 tcttcaggaa ggggaatatg agagagtagg cagcagtaat actcaaagag taaacgtgag
     4501 agtgatagct gccacaaaca gaaatctcga actttctatc aagcagaata aattcagagc
     4561 cgacctgtat tatagattaa atgttgtgcc tatacttgtg ctcccactaa gagaacgtaa
     4621 atccgatata cctcttttag ttaattattt cataacgcaa tactcgaaaa aattaggtaa
     4681 aaatatacgc cggatttctg ttaaatctca cgataagctt ttgagatata attggccggg
     4741 caacattaga gaactacaaa atgttttaga gagaaatatt gtactttcga agggaagtac
     4801 acttcatata gaagatcagt ttattgataa tatagaactg caggataacg tgaatttaat
     4861 gaggctagag aatatagaaa ggaatcatat tgttaaaatc cttgaaaaaa ccaattggaa
     4921 agtgcatggg gaacaaggtg ccgcttctat attggatata aatccgagta ctctcagagc
     4981 acgaatgaga aaactgggtg ttacaagacc ccagtaatct caatagtaaa cttctttaat
     5041 gaaatctcta tatattatag aaaatattac tttccaagca tattacccag cacactggat
     5101 agaccgcctt tcatgaggtc tgccatatct acgtctccgt caccgtctac atcaaggagc
     5161 tgattcagtg gatttgatgc ctggggctgg cttctttcaa agcttgctct ttctgtattt
     5221 agcatatcgc taaggtccct tgagttcatg ttattttgcc tgcgtgattt tcccaaggct
     5281 cccataataa tcggtgcaag tgtaacaagg agctttccta tagaacctgc atcaagacct
     5341 gttgatctgc ttaggctgga ctctactctt ggttgtctgc caccaagcat gtgtcttagg
     5401 ataccacttc ctgatccgga ttcaggattt tgaaggaata catcaaggtt atcgagaatt
     5461 ccgccatcat gatcgcgaga aagtgccccg agaagtttct ctgctccatc tcctctggtt
     5521 gcatttcttg ataacgcccc caataataca ggaagggcag cagacaacgc gttacctgtt
     5581 gttccttcat cagaacctat ctgtctgctg atctgaccta ctgctccact atttagtacc
     5641 tgtcctaaag tttctaataa tgctgacatt taatcccacc tcttatttta tttataccaa
     5701 gaatataata caatgtttaa gatgtgtaaa tgtatttttc gtacatagaa tgattgtaga
     5761 taattgaacg ttgtttcaaa aataatctgc cggactgtgt tatactttta ctatcataaa
     5821 agttttacat tttttcattt gagaatatgg gcaataccag tgaatttcaa aaattgctta
     5881 atgaacatct tatgcaaaca gcgggggttg atactgaaaa gcagctgagc gataacgata
     5941 aaagtatcct gttgaagatt gcaagaaaca cattgaagac ttttttggag acaagagaaa
     6001 tcccagaagt cgtcagcgat caagcatctt tactacagcc caaggcaaca tttgtgactc
     6061 ttagaaaaaa agacacaaat gagctaagag gctgtaaggg tgaaatattc ccctcaaaac
     6121 ctcttgcaga agctgtgcaa aacacggcaa tatcctcagc tacgagtgac ccacgttttt
     6181 gtgaagtatc ggccggggaa ctgccgaata ttaagattga gataagtgtt ttaaaaccta
     6241 taaagccaat aaaacctgaa gaagtagtta taggaagaca tggtcttgta atatccaagg
     6301 gcagcagaac gggactactc ctgccgcatg tgccgattgt gtataacctt gataaggaga
     6361 cttatcttaa ggaaatatgt tttaaagcag gtctcaaaga aaacgcattg gaggatgaaa
     6421 atactgattt atacggcttt gaagcagagg tatttgagga gtctgtataa attaatcgat
     6481 ttgtggtctt attacatttg cgaaagtttt catagtgttt ataaaattac taatgttact
     6541 gcttaatatg tatataatca tatggtctgc accggcagat ttataatttg ataaaccttt
     6601 tataattttt cccttgtttc ctctaagggg ttcatcgaca ttcgcattta ttgtactatt
     6661 atctgtaatt tgaagatttg ttcttgtaga aatactaaag gtttctcttc ttgttactgt
     6721 tttttcgaat tttctttcta tatattttct tttttccatt aattcatctg cattaagcct
     6781 gactgcatgc cagccggagc atttatcaaa tgccatatta atggatttaa tcgaattacc
     6841 gcccaaccaa attggaattt ctctgttgtt gtatgttctt ggcttaaaac taatattaga
     6901 aaaactaaag tattttcctt tgtagtcctt tttttcgcag taccatagcg attgtaatat
     6961 atctatatat tccagagtct tactccatct gttttcgtag tttgctccaa gtgcttcgaa
     7021 ctcatcttca agccagccag cacccacacc cagtattaat cttccttctg atagctgatc
     7081 aatcgtggta actgcttttg ctgtcacaat cggatttcga taggggagga caagtatgct
     7141 tgttccaaga gtaatagtag ttgttatgga tgctaaatat gaaagcgttg atacgggatc
     7201 ataaaagttg ttaccaaata cattgtgact ttccggaata acaatatggt cgcttaccca
     7261 aatagaatca aaaccatatt cttctgctgc aagggcaata tctgtaatat tgtctttagt
     7321 ggcatacttt ccaaaattgg gaaccgctat tcctattttc atgcatttga tagatattaa
     7381 ttaattgatt tatgatacta tttattgatt atcacatata cttcataaac gtaatttaat
     7441 aaaaaagtga aatcaaatat tgaaataaag gccaggatac aaaatctgca tgaagtacaa
     7501 aaacgtataa atgaatatta tggctctgat tatacagttt taaatcaaaa ggatatatat
     7561 tatgggtgta acaccggcag gcttaaacta aggatatttt ctgaggatga agccgagcta
     7621 atttcataca gaagggataa tatcaaaggg cccaaaaaat cagactactc tatttataaa
     7681 acgaataatc ccggacaatt aaataaggca ctcgaaagtt cactaggcat aataggagtt
     7741 gtggaaaaaa ggcgtttggt ttatattacg ggacagacac gaatacatct tgatgatgtt
     7801 gcggggcttg gaaattatct tgagcttgag gtcgtattaa aaagtggaca aagtgctgat
     7861 gaaggattgg ttatagcaga aagtataatt aaagaacttg aagttaaaaa agaaaagctt
     7921 atagaaggtt catatataga tttattatta aatagaaatg aaacacagaa aatctaaaat
     7981 aaaacgccaa cacagtctta taaaaggcct gcaggattat cttgaaaaat atatctcgag
     8041 ccttgagtat gttactggtg taattcccgg tgaaataaaa gtctcaaagg caacaggcga
     8101 aaacctgcat gtaaaataca aatatgaaac cgtaagcggt gctaagctta ttgcaaaaag
     8161 cggtacatca attcaggaag tatttgtgat tacaaaagat ccgcaaaaaa tagaaaagct
     8221 gtttgctgaa ctaaatccta gctgatagag actgtccctt tgcctagcat agtctctaga
     8281 tggtctatta acggatcatc tatactaacc ctatattccc caacttctat aacagctttg
     8341 ccatcttctg tttctatgtt taggttgatt tttgaatccc ctgggaattt taaaaatgca
     8401 tcctgcagct ttgagtagta ttcactgtta gccttttgct gttctaactg aacatttaaa
     8461 gtagacatac ttttgagctc ttttagtgag cttatgtcca gcgatcttag tttggttctt
     8521 tcttcagtgt gttcaactat accttttagt attatgggtt cagtcctgtc ctctaatata
     8581 tggagtgatt ttctaagcag atcattaaaa acaacagcct caaccgatcc cttaagatct
     8641 tctaaaacaa ggtttccaaa tatacccgaa ccgctctttg tgtttcttac atttagtgtt
     8701 cttaccactc ctgcaataga agcctcttta ccatccggga tttcctttat gctttcgctg
     8761 tcttttattt taacgagccg tgcaagatca tgtgcatatt tttccatagg atggtttgtt
     8821 acaaaaaaac caagtacttc aagctcgcac ctaagtatgt tcttttcatc ccagttctcc
     8881 gtatctgcaa gttctggaag agatatagag tcttctaacg ggaataagga attttgattt
     8941 tgtgaattta gttttgtgtt aagtgctatg aaatttaaga gcatttcaat agattcatac
     9001 agctgacccc tgttcttatt tatggagtcg aaggcgccgc tctttactaa actctctaaa
     9061 gattttttat tgatcttctt tgagtccata ttcttgcaga actgaaagat tgtagaaaac
     9121 ctaccattct cttctctctc tttaattatt gaatctatcg cactctctcc aatatttttt
     9181 attgctgaaa gtccaaacct gatcttgcca tttactgaag taaatccaga taaactttca
     9241 ttcacatccg gtggcagaat ttctataccc atatccctgc attcagcgat attagcaata
     9301 atcttatcgg tattgccggc ttcaactgtc ataagggctg ccatgaattc aggagcgtag
     9361 taggtcttca ggtaagctgt ctgataggtg atcattgcat aagccgtact gtggctctta
     9421 ttgaatgaat actcggcaaa cttttccata ttatcgaata tctcttttgc cttttttgga
     9481 gcaatactcc tgttcttgct tccttctaaa aatcttttcc tctgcgcttt catctcggca
     9541 ggctttttct ttcccatggc gcgccgtagt aaatcagctt ctccaaggct gtattctgca
     9601 acaatggatg ctgtgttcat tatctgttcc tgatacacaa aaaggccata agtgtctttt
     9661 agaatttcct gcagatcggg aagcgggtag tccacttttt tcttgccgtg ttttctttgt
     9721 ataaattcat ctaccatgcc gctatcaagg gggcctggtc tgtaaagtgc aagaagggcg
     9781 ataatatctt caaagtcagt aggctgcagc cttgaaagca cctctttcat tcccggtgat
     9841 tcaatctgga atatccctct cgtaaatcct ctggacagaa gtttgtaaac attaggatca
     9901 tctagtggaa tattcgaaat attaaagttc tttgcttcag gatcatcaga ctgttttata
     9961 agctgtattg ccttatttat gacagtaagt gttttgagcc caagaaaatc gaacttaaca
    10021 tacccaagag attcaatgga gttcatatca aactgggtta caatttcgtc ctttgctcct
    10081 ttatagagcg gtacataatc agatagaggc tcgttggata taacaacccc tgcagcatgg
    10141 gtcgaagagt gccttaccat attttccaga ggttttgcaa gctcaattaa ctcggtcaag
    10201 tcctctgagc tgctaataag gtccttaagc tccggtacat tctttattgc ttcttcaatg
    10261 gaaaatactc ttcctctaaa agacgggata agtttggaaa ctctgtctac atctgcatat
    10321 ggaattccca ttacccttcc cacatccttg accacggcct ttgcggacat agttccgaat
    10381 gttcctatct gagctacctt atccgctccg tatttatctg taacatatct tataacttca
    10441 tcccttcctt caccgcaaaa atcaatatca atatcaggca tgctcactct ctcggggttt
    10501 aaaaatctct cgaatatgag attgtatgga ataggatcaa cctcggtaat accaagcgca
    10561 taagctacca gacttccggc agcacttccc cttccgggac caactggtat gccctttgat
    10621 tttgcatagt taataaaatc agcgactacc agaaaatagc ccgcaaatcc catctcgttt
    10681 atggtcagaa tttccctgtc tattctttcc tgatatattt tcttttgatc accatccaga
    10741 ctcttatctc taatagtctt gttcagattg tcttttgtaa gctccagtaa gtattcttca
    10801 agcgacttgc cgttatcaat tttaaatacc ggaagcttgt atcctgtggt ctcaaattca
    10861 aaattgcacc tttctgctat ctcggctgtt ttatagattt catcctcata ttttccaaga
    10921 gaagaaacca tatcctctgc tgatttaaaa tagtattctt cccctttaaa cttaaacctg
    10981 ttttcatcct gcagcatcga tccggtctga atacaaagca gcgcatcgtg aggcttggag
    11041 tcgtgcttgt ttaaaaaatg gcaatcattt gtggccacta tgggtatttt tagagatttt
    11101 ccgatctgtt tgatcttctc atttaccaat tcctgctctt gcaggtcatt agcctgtact
    11161 tccagatagt atctgtcacc aaacatctct ttatatttag atgcaacggc catggctttg
    11221 ttcatatcat cttccagtat gcttgtgcag agctcaccat taagacagcc gctaagtgat
    11281 attattccct cactgtgcag ttccaaaagc tcatgatcta tcctgggtct tctgtaaaat
    11341 ccgtcaaaat atgcttttgt cactaggttt gatagatttt tatatcctgc tgtatttgtt
    11401 gcaagaagta tcaaatggtg atttctggcc tgagaagggt gatcaacttt cagggtagga
    11461 gttatataaa tttcacatcc aattatgggt tttataccaa actcttttgc cttttgatgg
    11521 aattcatagg cgccaaacag atttccatgg tctgtaatgg caatagagtc catttcatac
    11581 tcttttgctt tcgtaaacag atcattaaat ttaattgcgc cgtctaaaag cgagtactga
    11641 gaatgtaaat gtaagtgaat aaactggtct gacatataaa atctatatta tctgaatata
    11701 agtctaaaaa aagtaaaaaa ttattaataa atccaaattt tgaatcaagc ttattgatat
    11761 gagattttgt aatagtatat taaaggtcaa tatatgaatt tttactaata gagccagaaa
    11821 tctaaggtat gaaggagtag tcgatttgga atcaataatt aataacttat ccagcacttt
    11881 aaacatgaat cctcaggatt taactttcat tctaatatgt gctgtcatat ttttagtaac
    11941 tttaatttta gcaatatttg taaaggatga agtaccggag gtatctgaac cagaagaaga
    12001 cagttctcaa gctgctgaag atgctgttgt tgctgaacaa ttacaacctg ctgatttaga
    12061 agaaattcag ggtaaagtag agcaacaaac cgaagtgtcc gaagaggttg tttcggaacc
    12121 ggctccggaa tttgtagcta agaccgagcc aagggaatct gaagcagccc ccaaggtgga
    12181 agttaaggtt gagcccgtgg ttccacctca aatacctcaa gagatagtag aggaagttac
    12241 agaagaacag ccggatgaac agatagaaga agctcctaag gagagttttt tctccaggct
    12301 ccgaaaaggt ttatcaaaaa ctcgtacagg gtttattgat aaactcgatg caataatcag
    12361 cagaaccgag attgatgacg agatttggaa agattttgag gagacacttg ttatggctga
    12421 cctgggagtc acaactacaa tgaaattaag agaggatgtg gaaagagaaa tagagagtaa
    12481 ttccattact gatcctcatg aaataaaaaa tatactcaag gccaaaattc tagcgatact
    12541 aaaagaatcg gaggggggtg cagtaagccc tcaaacaaaa cctttcgtaa tgatggtggc
    12601 cggggtaaat ggagtaggaa agacgaccac tatcgggaag ctttcaaaca aattcaaaaa
    12661 tgaagataag aaggtcatgg ttgctgcggc agatactttt cgggcagctg ctgtagacca
    12721 gctcgaagta tgggcaaaga gggtaggcac agactttata aggggtcaaa gcggagcaga
    12781 cccatctgct gtagcttttg atgcggttaa ggcagcggtt tccagaggtg ctgatatact
    12841 aattatcgac acagccggaa ggcttcatac aaaagacaac ctcatggatg agcttaagaa
    12901 aatgagaaag gtaataaaca aagaacttga gggagcacct catgaaacga ttcttgtgct
    12961 ggatgctaca acaggtcaga atgccataca gcaggcaaag ttgttcaatg aagcaattga
    13021 ccttactggg atagcgctta cgaagctgga tggaaccgct aagggtggtg taataattag
    13081 tattgcaaac gagcttagta ttcctgtgaa atatattggg attggcgaga ggcttgaaga
    13141 ccttagagaa tttaatgctg agcaatttgt agaggcactt tttggtgacg aagaacatac
    13201 tattcactaa cttcatctat gagcaatgat attgatgcta aatatatggc tcttgccatt
    13261 aagcttgcca aaaaagccag tggaatgaca agcccaaacc cggttgtcgg ttcagtgatt
    13321 gtaaaaaacg gcgagatcat cggaaagggc tatcacaaaa aggccggact gcctcatgca
    13381 gagatcgaag ctatcaacaa tgccaggaaa cagggcaaaa aaattgctgg ctcgactctt
    13441 tatgtaaccc ttgaaccctg ctgtcacaaa aataaaaaaa caccgccgtg tttggatgca
    13501 gtacttgaag agaaattttc tagggttgtt attggttctt tagatagaaa ccctcaggtt
    13561 tcaggcaaga gcgttaaggc tcttaagaga aaaggggtag aggtctcagt cggagttcta
    13621 gaagatcaat gcttaaagat taatgaagca ttttttaaac atataagcaa aaagattcca
    13681 tttgttaccc tcaaactcgc atctactctg gatggcaaaa tagccacttt ttctggtgat
    13741 tccaaatgga taggaagtga agggcaaaga aaacaaggcc atattctaag atccctttca
    13801 gatgcagtat tggtgggcat caacaccgtc aaaactgacg accctatgct gaatgtaagg
    13861 cttgaaaaaa agagagtgga taaacagccg atagcagttg tcctcgatac caatcttgag
    13921 attccgcttt cggcaaaact tttaaccgag cgttctggaa caatgatctt ttgctctaag
    13981 aatattaata aggttaagaa aatgaaaaga cttaaagatt tggggtttgt agtagtaaaa
    14041 acaggccttg ataaaaacgg ttttgtaaat actaaagagg ttctaaaaaa gcttgggtca
    14101 agaggtatca tgagtgtttt ggttgaaggc ggtagcaagg tggcatcgag ctttataaga
    14161 tacggacttg tagataaagc catatttttc tatgcgccca agattgttgg ggcagatgga
    14221 aagagcatga ttggtgaact tggaataaat gaaataaaag actcatttga actctcaaat
    14281 gtaaatataa gttcaattgg taaccacgtt atggttgagg gttatttgaa ctgaaatgga
    14341 agagcatcta tcgtgaaaca aggacttttt ggcggcacat tcaatcccat tcactcgggc
    14401 catgtaaagg ctgctgagca aatcctcgaa attcttgata ttgatagaat atgttttatt
    14461 ccatcgaata ttccgccaca taaggatcaa aaagaactag ccacaccgga gcaaagatac
    14521 gagatggtta aacttgctac tcatggcaac gatagattta gtatctcaga cttcgaaatt
    14581 tcaagtaaca aagagagtta tactattgat accctcaaat attttaaaga caaattgccc
    14641 gaagatgatc tatattttat tgttggacac gatatcttta accagattga tatttggaag
    14701 gaatataaga aattatttga gttatcaaat tttatagtta tttccagacc gggctacttt
    14761 gatgatgatg aagatctgcc acttgccata aaagatgatt ttcgatatga taagacagaa
    14821 actggaaata catattacaa acacaaaaat tcaaatttga ttatcaaaac gcaaatagag
    14881 ggaatagata tttcatcttc ggagattaga gaactcatta aaaataataa accgatacat
    14941 gagcttgtcc cgcctaaagt agaacagtat atattcgaca acaaccttta tgcctaaaaa
    15001 cggatcccta acaccaaaga aaaaagctct tgccctagct gaagctgcct acgacaaaaa
    15061 agcagaaaat atagtcttgc tccatatgac aaagctatct gattttgcag attattttgt
    15121 aatatgcagc gcagattccg accgtggtgt aaagaccatt gtcgaaaata tcgaaaagaa
    15181 atgcaaggag cttggctcaa aacccctggg aatagaaggg ttaaaagagt gcaggtgggt
    15241 gcttatagat actggtgata ctcttgtcca tgtttttcat gggcctgtaa gagccgaatt
    15301 tgacatcgaa agcctgtgga acgaagcccc aagggtaaag cttccgtttg agcaggaaca
    15361 ggttgcgagc taaacaatta tagtgaaatt atgaaaagat ggttagtctc ggttcttatt
    15421 atattttcaa taatatatat ctgcacattt ttatcaatta agtacaaata tactcaagtc
    15481 tcttcagtac aatccaatta tattttactc gaagagagca agatttctca taaaattata
    15541 catattattt tttatccggc attgtggatt gattatcaaa ttacaggaca agaatacggc
    15601 ctcataacta ataaagacaa tccttactgg atatttgccg attaattaat cattcttaag
    15661 cactgcataa aaagcttcct gagggatttc tatgctgcct acctgcttca ttcttttctt
    15721 tccctctttt tgcttttcaa gaagttttct ctttctcgaa acatcaccgc cataacattt
    15781 tgccgtcaca tcttttctta gagccttaac ggattctctt gaaatgatcc tgtttccaat
    15841 tgctgcctga atagctacat catacagctg tcttggaatg agtgaccgga gtttctcaat
    15901 aagtgctttt cctctctcgt atgctttttc tttatgagtg ataacagaaa gcgcatccac
    15961 ggggtcaccg tttataagta tctccatctt aataagatca gaggcccggt agccgattgg
    16021 ttcatagtcc attgaagcgt agcccttgga gattgatttg agtgtatcat agaaatcata
    16081 aactatttca gagagcggaa gttcatattc caccaacact ctgtcctctg ttttatagct
    16141 caggtttttt tgaatccctc gcttgcttct gcaaagatca aatatagcgc caaggaactg
    16201 atttggcgta tgcagggtga cttttacata aggctcttct attatctcaa acttatccgg
    16261 tgtgggaagc ttgctcgggt tttccacttc agtcattttt cctgtcttgt ctattaccct
    16321 gtatactaca gtgggagcag ttgtgataag atttagatca aattctcttt ctaaacgctc
    16381 tttgataatt tccatatgaa gcaggccaag aaaaccacac ctgaagccaa aaccaagagc
    16441 aaccgaagtt tctggctcat aggtaagaga tgcgtcattt agcccaagct taagcagggc
    16501 atcttttaat ttctcgtaat catttgtgtc tattggataa agtccgctaa ataccatcgg
    16561 ctgcacttct ctaaacccgg gtatgggttt atctgttgga ttttccaaag atgttatagt
    16621 gtcaccaacc tttgcctctt ctatatcttt aatcgatgcg gttacaagac ccacctcgcc
    16681 cgttccaaga gtctttaaag ctatagcggt tggagaaaaa actccaagtg tttttacatc
    16741 aaaagattta cctgtagaca taaacattat attttcgcca acatttatgg ctccttcaaa
    16801 cacccgaacc agcatcacta caccctgata tgaatcgaac cagctgtcaa atataagcgc
    16861 cttaagaggt tttcccctat tgtcttgagg tggaggaata aggtgaataa tggattcgag
    16921 tatttccttt ataccgatac cggtttttgc acttgcaagt gtcgcatctg tagcatcaat
    16981 acctacaata tcttctattt cacgttttac cctttcggga tctgctgaag gaaggtcaat
    17041 cttatttatt acaggaatta actccaaacc aatatctaca gcaagataag catttgcaac
    17101 ggtttgggcc tctacaccct gtgaagcgtc cactaccagc agcgcccctt cacaggcttc
    17161 caggcttctg gatacttcat agctgaaatc cacgtgcccg ggggtatcaa taagattgaa
    17221 ttcgtataac tggccgtctt cagatttata attaatcttt acagactgtg ccttaattgt
    17281 aattcctctt tctttttcca gatccatttt gtcgaggaac tgttcttcca tgtccctctt
    17341 ctcaacggtt cccgtatact cgaggattct gtcggctaga gtagattttc cgtggtctac
    17401 atgtgcaatt atgcaaaaat tcctaataaa tttaggatcc attcacaacc tatataaaag
    17461 ttagagatgt acgaataatt atttttatga ataaactaca tgttacgcag gatttcactt
    17521 gctactttag tgcctgcacc aaactcgaac tcatgaccca gatttttaag aactgattct
    17581 atagcggaga taacaccaat ggtatcagct ctgtctatat aaccaatatg cgacatcctg
    17641 aaaatctttc cttttgcatg atcctggccg cctgcaagag ttattccaaa atcactgcta
    17701 agagagctta tcattttctt agcgccaatt tcttcaggag cataagtgac gttcagagct
    17761 gtgcttggtg tgccttttac aaagccttca agccctattg ctttcattgc ttcccttgtg
    17821 gcaagtgcta gtttctcatg cctgttatgg atattttcaa ggccttcagc cctaaagttc
    17881 ctaagcacct gagcaagtcc gattataagg cttaccgcag gggtaaaagc tgttgtgttt
    17941 tttaaagcat ttttctcaca gctcttaaaa tcaaaataaa actttggaag atccgatttt
    18001 tcataaaatc cccaggcctt atcactcatt gcagcaaatg acagtccggg aggtagcata
    18061 aaagcctttt gcgaaccccc tactaaaaca tctattccat attcatcaaa aggcagtggg
    18121 aaaacacaaa ctccggttat tccatcaact attaatatta catcgtctct ttttttggtg
    18181 atctgtccaa tttcctttgt cgggtgcata acacctgtgg aggtttcact ggcctgtatc
    18241 attacagcct tgatatcttt atcgttattt agatgttctt caatcctggc agggtctacg
    18301 ctgtaacccc attcaactgt ttcttcaact acttctagcc cgtaagcctt tgaaattttt
    18361 ccccatcttt ctccaaattt accgccgttt actaccagta ccttatcacc tctgctcaag
    18421 gtatttgcca ctgcggcttc cattgcacct gtgccagatg cagccagtat gtatacttct
    18481 ttttgagttt cataaacgta ttttaatcct tcgcgtacct ctgcaaaaat actttcaaat
    18541 tcttcagttc tgtgatgaat aataggtttt gccatttgca gcaaaacttc ttccggcaca
    18601 gacaccggac caggggtgaa aagataagtc ttcaatgtta atctccttaa ctaaaatttg
    18661 cgaaaaattt accctaatcc tgttataatt caaaccccgc gcagatataa atcaaacttg
    18721 ctattagata tggatcaatt tatattctaa acaaatttat atatagccat gtttacagaa
    18781 aacaaagaca aaattcagga tataaagaca agactaaagc tgctgggaga ctatctttga
    18841 cctgcctgca aaaaaagaca gactctatga actcgatctg gaagtaagcc aggcagattt
    18901 ttgggataat aaagaatctg cacaaaaggt gctgaaagaa cagtctcaga taaaaacaat
    18961 tgtaagtgac tggagctcct tatctgaaga actcgaagaa gccgaattgt tatctgagct
    19021 cagcgccgaa gaaaacgatg aaaccacggg ggccgaagta cagggcaagt tgtcagcctt
    19081 atacaaaagg cttgaatcac tcgagtttaa aagaattctg ggtgagcccg aagacccgaa
    19141 taatgctatt atttccataa attcaggagc agggggtaca gaggctcagg actggacgga
    19201 gatactcctg cggatgtacc tgcggtactg tgagatgagc ggttttgatg tgcagatgct
    19261 cgataaccag cctggtgatg atgccggggt aaagagctgt acgtttttgg caaagggtcc
    19321 ttacgcgtat ggatacttaa aaggagagag cggcgtgcac aggcttgtgc gcatttcacc
    19381 atttgattct aacaaaagac ggcacacttc gttttcgtcc gtatttgtta cacctgaaat
    19441 cgaagacgat atagatattg agatcgaaga aaaagacctg cgagtagata catacagggc
    19501 ctcaggagcc ggtgggcagc atgtaaacaa gacagattct gcagtaagaa taactcatat
    19561 tccaacaaat attgttgtta gctgtcagaa tgaaaggtct cagcaccaaa acagggctac
    19621 ttgtatgaag attttaaaag gcaggctcta tgagcttgaa aaacaaaagc agcaggaaaa
    19681 gctcgacaat attgaatcat caaaaatgga aatagggtgg ggctcccaga ttcgctcata
    19741 cgtgtttcat ccatacagga tgattaagga tcacaggacc ggttttgaaa caggaaatgt
    19801 cgatcccgtt atggacgggg accttagtga atttataaaa aattatttgt tgcactcttc
    19861 taataataat tagataatta aatcttatag gagagatttg aattgaaagt aggaatagtc
    19921 ggcaaaaaag ataatagcga agttattaaa cttgcaaaat tgcttataga aaaatttggt
    19981 tcacaaggga tcgaagtctt tttagaggac gaactggctg tattggtgga tcatgctaat
    20041 tcaactaaag ccgagaaaat tcaagagaaa tctgatgtgg tgatcgtact tggcggtgac
    20101 ggtacgttcc ttggggtttc cagaaaccta agcggcacag atgtgcctgt tgtaggcatt
    20161 aatatgggaa acctgggatt cctcactgag gttacaagag aagaggcgct tgagcttgtg
    20221 gatatcatcg caaagggtac ttatgatatt gaaacacgcg atatgataac agccagggtc
    20281 cttagagatg gaaagcagtt ctgcaaatat gatgtactta atgatgtggt tataagcagg
    20341 ggtgtagttt ccagagttgt tgacctttca gtagaaacta ataataagca gattactacc
    20401 gtaaaggccg atggtctgat actatcaact ccaacaggtt ctactgcata ttcactttct
    20461 gcaggagggc ccatcgtgtt tccaacccta ccacttatga tcataacacc aatttgtcct
    20521 catacgctta ctaacaggcc gcttgtagtg tctaatgaga ggaagattag agtcagatcg
    20581 ctgtctgaag aaacagagct gcatcttaca ctcgacgggc agattagttt tgatctgatt
    20641 cctaatgatg ttgttgaagt ctcaaagtca gaaaaaacag tcaaatttat taagtctcca
    20701 ttcagggatt attttacaat tcttaaaact aaattaatgt ggggtgaacg ttatggcacg
    20761 ctctgaagat gaacagttcc ttcatgactg ggttataaga aagataaaag agaaaaatgg
    20821 cagaatgtat agcgaggtca atatcaatcc gggtgatgag cagaattacg atttagaggg
    20881 taaatatccc gatgttgtga tggtaaatca tggtcaggtt gtgggcataa tagaggttga
    20941 tacaaaagaa acagtagacg agagctgtgt ggacaagtgg aaagctctat cggagcttgg
    21001 agcaaaactc acacttgtag ttcccaaaga agaacagaag aaggcacgcg atcttgtatg
    21061 gaaaagcgga ctcgttgcaa aagtggacat aaagtttttt gatgtacata taggtgtcta
    21121 accaagtctc attccacttt ctatttttct tccggcaaga aatgatctga tttccatttt
    21181 cttgtttccc tcaagctgca gctcttttaa gtcaagactt ccggatccgc atgcaattct
    21241 aagaatactt ttacttgctt ctaatactac tccgggatca ccgcttttct cggaaacatc
    21301 ggcgctaaat atttttagca tctttccgtt tagattcgta tatgctaccg gccatggcag
    21361 aagacctctt atctgatttc tgatttcagt ggctgatttt gaccagtcta tttccccgtg
    21421 ttcttttttg agtatgggtg cgagggtagc ttcactatca ctttgcttta tgggattgag
    21481 tccctcttgt tttaattcat ctattgtctt gattaataat tctgcgccaa tttcagataa
    21541 cctttttgta agggtttcag aggtatcgtc agcagtaatg ttagtttctt caataagcaa
    21601 tatgtcacct gtgtccatac cttcatccat tagcatggtt gtaacaccgg tcctggactc
    21661 accgtttatt attgcccaat ttattggtgc ggcaccacgg tattttggca gcaatgatga
    21721 atgaacatta atacatccat gcactggaag gtctaatact tcttttggaa gaatctttcc
    21781 ataggctgct acgcaaatta gatcgggcgc tagttgttta agctcattca gaaatttttc
    21841 attgtttcta atttttgcag gttgtagtac tgaaatattc cggctttcgg caaactgttt
    21901 tgttagaggc ttgtgtaatt ctctttttct gcctaccggc ttatcgggct ggcagacaac
    21961 agcaattact tcatcctctg actctgtgag tgccttgagt gatggtacgg caaattccgg
    22021 ggttcccatg aaaatgattt tcatcttctc ataccactga gttaaactag ttctgcgtgt
    22081 aaattgtata tatctgcttc ggtgattctg acttgcagga aatcaccttt tttaagtttt
    22141 ttgtccgtag ttatatacgt cagtccatca acctcgggtg cctgagtgct tattctgcca
    22201 aaataattgc cgtcctctat cccctcgaca aaataaccaa atgttttacc tataagcgct
    22261 ttattatttt caagcgatat atcggactga acattcagaa gtctttcgag cctctcttcc
    22321 ttaatgtctt catcgatctg accatcaata tcaaaagaag gcgtaccctc ttcatgcgaa
    22381 tacctaaagg caccggctct ttgaaacttc gtgtcctcaa caaattcaag cagctcttca
    22441 aattcatctt cagtctctga cggaaagcct acaataaatg tagttcttaa tgttagatcg
    22501 ggcatagact gctttaattt atttacaata gataaaattc tctttttagg ggtttttcta
    22561 tccatcagtt ttaatatcct gtcatttata tgctgtatgg gcatgtcaat atatggcaga
    22621 aggttattgt catctgctag cagatcaaca agctcatcag ttactcccca agggtagcaa
    22681 taatgcagcc tcatccattc tatagaatca attgttataa gttcttttaa aagggtgtaa
    22741 agacttgtgc cgatgtctct gccgtagctt gtcatgtcct gagcaattag atttatttct
    22801 tttactccgt tttgtccaag catagatatt tcattatata ttgaatcaac actcctgctt
    22861 ttcatgattc ccctcatctt gggaataatg caaaatgagc aggttcttga acagccttct
    22921 gatactttta cataacttgt gtgagaatca gtgagcatta cccttgggct gtcggggtca
    22981 tagattgttc caaaaggggt tttgttgaaa tcgtaaattg tatctgagag tacttcatta
    23041 atctttagca ggtttccggt accccagaaa tgatcaacct cagggaattc gtcttttagc
    23101 tgctgcgagt atctttctac taaacagcct gtgactatta gttttttgca tgagccggtt
    23161 ttcttatact ctgccagatc aatgatcgta tttatagatt ctttttttgc atcgcctatg
    23221 aatccgcatg tgttaattac aattatgtct gctttgtctt cagtagtgat ctcgtggcct
    23281 gagtttgtga ggcttccgag tataagctcg ctgtccacga ggtttttaga gcatccaagg
    23341 cttgtaagtg atattttcat aatattatta agcgcttaga taataccaga aggctcattg
    23401 ctaaccaatt aattatagca attatattat tttgaacgaa tattcaaaaa aggtgaatct
    23461 ttttatatag taatttgtct aaatatataa ataacaattt ggaggtaact aaaatgaaaa
    23521 agttactagc agttacagcg atagcattat caatggggct tagctcaata agctttgcag
    23581 acacaaatgt atttggcgta aatgtgcctg ttaaaaagaa tcaagtaagt gatcatataa
    23641 agggcagtta tgtagagtca gatcatatca gcttttatct caatgccaaa gacagcgtgg
    23701 atgtaaattc tactgaacat gcaagaagat ttagtttgga agattacggc cagtatgtat
    23761 ttggagtaaa gatttaatga ctgtaaataa acagggtatc aaatctcctt atataaacgg
    23821 agcacggggc aggaattacc tgccctacgc tcttttttta atctgatgtg aaacttttcc
    23881 gtttctatct gctttaatgt taatttgccc gctgtttcct ttgccaaaaa gctcaaaagc
    23941 aacgtcttca atatacttac taagcaaaac atcaagaccc cttgcgccgg tctgcctttt
    24001 tatcgattca tccacaacca ggtctgcaag gcttgaatct atattaagtg tatagccttc
    24061 ctgaataaac tctcttttat agttttcaac ttttagtatt agaatatcct ttagagtatc
    24121 cctgccaagg ggttcgaacg ggataattct attaaacctg gcaattagct ccggaaggaa
    24181 tccaaagagg tggaactgtg aaatatcatc ggcctgctcg tctgatattt tatatgcaat
    24241 gtcatatttt tgatctgcgc cctgagccag tttttgttca aatcccatac cgtcttctct
    24301 aaacatatcc ttaatccccg agaatgctcc aactgcaaaa aatgaaatat cccttgtgga
    24361 catcatagcc ctcgggccgc ggtttgaaaa accaaaatcg agcggtatct gaatatccgt
    24421 gccctccagg atttttagaa gttccctctg taccccatag ccggatacat cctttgtagt
    24481 gccctggcct gcaaagtgtg cattgctgta tgagcctgca agcttatcaa attcatcgag
    24541 cactaccact ccgcactccg ctatatccag gttttcattt gatgcataaa caagctgaac
    24601 cagtatattg agcacatcat cacccacgta gcccgactca gtaaactttg tcatgtctat
    24661 gatgacatat ggaattttaa gtatctgccc aaaaagcagt tcaagtatat atgttttgcc
    24721 gcagcctgtg gggcctatta gaatagagtt tggtctgggg gggagatc
//
LOCUS       DBNGSV010000354         2429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_618687, whole genome shotgun sequence.
ACCESSION   DBNGSV010000354 DBNGSV010000000
VERSION     DBNGSV010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_618687"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="AC159U_12690"
     CDS             <1..591
                     /locus_tag="AC159U_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337953.1"
                     /translation="THYPVPKTFLREGNILDYGYEDELLLVSDIIIPISYNGGNFKIT
                     TEANWVACKEVCIPGSENLELQYPVTNANNLAQNKLFNSWIAKKPHIINNDRLPYKYS
                     VNKEYNEEGKLKQVDITLNWDEEVKNVEIFPDVDKSVYMKDIIFNSNKNISTYSFSPV
                     IFNKNKKDLKELIIVISYDNIKGQRVGIETTLNLSS"
     gene            620..1192
                     /locus_tag="AC159U_12695"
     CDS             620..1192
                     /locus_tag="AC159U_12695"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3337954.1"
                     /translation="MRILLALLVFGLLFSVFSEVNALSIGDKAPDFSLQDQKGAMINM
                     ADYKDNVIVLEWINPDCPYVKRHYSEGTMKELAEKHGEKIVWLAVNSTYYMDSKANSD
                     WTNKYLIKYAILNDSSGNVGKSYGAKTTPHMFIVKDGVVVYNGAIDNDKWGKSEKRVN
                     YVDLGLGEVLKGKAITTSETKPYGCSVKYK"
     gene            1189..1584
                     /locus_tag="AC159U_12700"
     CDS             1189..1584
                     /locus_tag="AC159U_12700"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3337955.1"
                     /translation="MMNNLNDIGILVLRVSAGLGMLIAHGIPKIMKPEGFIGVLTDMG
                     FPLPKVSAYLAISAETIFPVMIVLGLLTRISSAIAAVNMFVAGIVFHLVMKGDPFSNY
                     EKALLYFLIFLILAITGSGKYTVEKLIKN"
     regulatory      1661..1761
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          871 a          338 c          430 g          790 t
ORIGIN      
        1 acacactacc cggttccaaa gactttcctt agagaaggta atatacttga ttatggttac
       61 gaagatgaac tgttattggt gtctgatata ataattccaa tcagttataa tggtggtaat
      121 tttaaaatta ctaccgaagc taattgggta gcatgtaaag aggtgtgtat accgggttca
      181 gagaaccttg aactacaata tccagttaca aatgctaata atctagccca aaataaactc
      241 tttaattcct ggattgctaa aaaaccacat ataataaata atgatcgact tccctataaa
      301 tactcagtta ataaagaata taatgaagaa ggtaaactta aacaggtgga tattacatta
      361 aactgggatg aagaagtgaa aaatgttgag atatttccag acgtagataa atctgtttat
      421 atgaaagata ttatatttaa tagtaataaa aatatttcaa cttacagctt ttcacccgtt
      481 atatttaata agaataaaaa agatttgaaa gaattaatta tcgtaatttc ttatgataat
      541 attaagggac aaagagtagg tattgaaacc acactaaatt taagtagtta attgtatatt
      601 aaaggggggt aataaagata tgcgtatatt actggcattg ttggtgtttg gattgttgtt
      661 tagtgtattc tctgaagtaa atgctttatc gataggtgat aaagcccctg atttttcgct
      721 gcaggatcaa aagggtgcaa tgataaacat ggcagattac aaggacaatg ttattgtgct
      781 cgagtggata aaccctgatt gtccttatgt taaaagacat tattctgaag gaacaatgaa
      841 agagcttgca gaaaagcatg gtgagaaaat tgtatggctt gctgtaaaca gcacttatta
      901 tatggatagt aaagcaaatt ctgactggac caataaatat ctgattaaat atgcaatatt
      961 aaatgacagt agcggaaatg taggtaaaag ctatggggca aagactacac cgcatatgtt
     1021 tatagtaaaa gacggagtag ttgtatacaa tggtgctata gataatgata aatggggtaa
     1081 aagtgaaaaa agggtaaact atgttgatct tggccttggg gaggttttaa aaggtaaggc
     1141 tattactacg agtgagacta aaccatacgg ttgttcggta aaatacaaat gatgaataat
     1201 ttaaatgata taggaatttt agtacttagg gttagcgctg gtcttggtat gttaattgcc
     1261 catggtattc ccaaaataat gaaacctgag gggtttattg gagtacttac tgatatggga
     1321 tttccactgc ctaaggtaag cgcttatctt gcaatctctg cagaaacaat atttccagtg
     1381 atgatagtac tagggctcct tacaaggatt agttctgcaa ttgctgcagt aaatatgttt
     1441 gtagccggta ttgtttttca tctagttatg aagggtgatc ctttctcaaa ctatgaaaag
     1501 gcactgcttt actttcttat ttttctgata cttgcaataa caggcagcgg aaaatataca
     1561 gtagagaaat taataaaaaa ttagcttaaa gttataaata atacctttgt attaaaattt
     1621 aagttgacaa gttaaaaaaa agtaatacaa tataaattaa gagacgaaag ggcaaaccat
     1681 ccgaaagggt gggacgcaaa gtttaaggcc tacagatagt tattagttaa aataattatc
     1741 aatggttgct tggctaccga aatgttgatt tatacctttc agtagtcccc ccttttactc
     1801 agaagtaatt gtattattaa ttgacaattg aatatatagc cattctctcc ttcattctaa
     1861 gttgtgagga tgagtgacct agaaataata aatttctaaa agccttcatc cttattcact
     1921 tgtcctcaca gcttaattaa taaaaacatc tacaatgcac taatttcaat atacttatat
     1981 atttgatttg gtttttataa attttacaat actatttcta agtttatcat ttgagcgtat
     2041 catgtaatcg gttaaatcaa actcaaacac atccatttca cttattatag aaaacttata
     2101 atcattttcg ttgtatctaa taaaatctct ccatagtaaa gtataatcag tatgtaaatt
     2161 tatttcattg ttgaaaaaat atttactgcc tcgtttgata cctaaatcat caaaaactat
     2221 tgtttcttta aatgcactat ataataggat gccagataat gagcatgata agatttttaa
     2281 caaattgtaa tcagaatata gtaatgagaa tatagagaat atagaaaaaa ctccccctaa
     2341 taatgaccct cacccaaaag ctgtaccggg ataattagag atttccggct tttttatgtg
     2401 ttgtttaaca tactcctccg gcgtcatat
//
LOCUS       DBNGSV010000355         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1379623, whole genome shotgun sequence.
ACCESSION   DBNGSV010000355 DBNGSV010000000
VERSION     DBNGSV010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1379623"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="AC159U_12705"
     CDS             <1..257
                     /locus_tag="AC159U_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR3337956.1"
                     /translation="LHNDYNIHMSPKKPIGDQPMEKSLTIRTTDDFLKALEEAKWQLR
                     MDKSMIIRKAVEEYISNHLPEKELKKINKYFKSSKKGGKK"
     gene            257..1306
                     /locus_tag="AC159U_12710"
     CDS             257..1306
                     /locus_tag="AC159U_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase"
                     /protein_id="HIR3337957.1"
                     /translation="MGVFKRLINSKKGKTPYWYIRYTVNKKPKWESIGKVGEVTKAVA
                     DAILAERKKQIRLGTYNLINTKIPTLNEIKDEYIQYKRNILKNRSWKKDESHIKRWSD
                     YLGNKLLSEITVKDIDNYKLQRQEEVKAVTVNRELQSLRHLFNLAKKWKKFFGENPVS
                     ESRLIKTESNRKRILTYEEEAILINHSSPHLKPIVQSALLTAMRLGELLTLRWKDVDL
                     KNNLITIRAEISKSKKQRKIPISKRLKEILKEQRYISGLSGMVFLTQDGKPYSPKNSS
                     ALKRTFTTARKKSDLMDLRFHDLRHTAATRMAENGASVVAVKEICGHANLNTTLQYFH
                     PDKSLTEAVEILANQ"
     gene            complement(1369..2112)
                     /locus_tag="AC159U_12715"
     CDS             complement(1369..2112)
                     /locus_tag="AC159U_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR3337958.1"
                     /translation="MKLFIQVCFTITLLMFLGINVQTASAGVPINTTERGWYDNLGDH
                     TPSNTNYIAGAENNEIFHDFFVFDLSSTQICAASLVLFNPNVGPGPFPGYISPDPTET
                     FSIYDVTTNIANLIAGTAGVAAYNDLGGGVSFGSTTVSAADNGQFVTVNLNNDGINAL
                     NAAAGGQIAIGGALTTQGQIFESELVFGSTNTDDPQDGNTFLNLEFCTTGPITIIPTM
                     GQWGIVIATILLGFFAVIRLRRVKDSELE"
BASE COUNT          941 a          390 c          409 g          671 t
ORIGIN      
        1 aattacataa tgattacaat atacacatgt caccgaaaaa acctattgga gatcagccga
       61 tggagaagtc attaaccatt agaacaacag atgacttttt aaaagcactg gaagaagcaa
      121 aatggcaact aagaatggat aaatcaatga ttataagaaa agcagttgag gaatatatta
      181 gtaatcattt gccagagaag gaactaaaga aaattaacaa gtattttaaa tcatctaaga
      241 aagggggtaa aaaataatgg gtgtgtttaa aagattaata aatagcaaga aaggtaaaac
      301 tccatattgg tatattcgtt atacagttaa caaaaagcct aaatgggaat ctataggaaa
      361 ggttggagaa gttacaaaag ctgtagcaga tgcgatttta gctgaaagaa aaaaacaaat
      421 taggttgggt acatataatt taataaatac caaaatccct accctaaatg aaattaaaga
      481 tgaatacatt caatataaaa gaaatatatt aaaaaacagg tcatggaaaa aagatgaatc
      541 acatataaaa agatggtctg attatttagg aaataaatta ttatcagaaa tcacagtaaa
      601 agacattgat aattacaaat tacaaagaca agaagaagta aaggcggtta ctgttaacag
      661 ggaattacaa tctctaagac acctttttaa tttagcaaag aaatggaaga aattctttgg
      721 agaaaacccg gtgtcagaat ccaggttaat taaaacagaa agcaatagaa aaaggattct
      781 aacttatgaa gaagaagcga tattaataaa tcatagttcc cctcatttaa agcctattgt
      841 tcagagtgct ttgcttactg ctatgaggct tggtgaacta ttaacactaa gatggaaaga
      901 tgtagattta aaaaataacc taattaccat tagagcggaa atttcaaaaa gtaaaaagca
      961 aagaaaaatt ccaattagta agagattgaa agaaatacta aaagaacaga gatatataag
     1021 cggtttatca ggaatggtat ttctaaccca agatggaaaa ccctacagtc ccaaaaattc
     1081 tagtgcttta aaaagaacct ttacaacagc tagaaagaaa agtgacttaa tggatttaag
     1141 gtttcacgat cttaggcata cggcggctac aaggatggca gaaaacggag cttctgtagt
     1201 agctgttaaa gaaatatgtg gacatgcgaa tcttaacact accttacagt attttcaccc
     1261 agacaagtct ttaactgaag ctgtagagat attagctaat caataaaaaa cccacaacca
     1321 aagggttagc tctgattatg ggttaattct aattaaattg ttattagatt attctaattc
     1381 cgaatcctta acccttctta acctgattac agcaaagaat ccaagaagta tagttgctat
     1441 aacaataccc cattgaccca ttgttggaat tatagttatt ggtccagtag tacagaactc
     1501 aagatttaga aatgtattac cgtcttgggg atcatcagta ttagtactgc caaaaacaag
     1561 ctcagactca aaaatctgac cttgggttgt gagagcccca ccaatggcta tttgtcctcc
     1621 agccgcagca ttgagagcat taattccatc attatttaaa ttaacagtta caaattgacc
     1681 attatcagcg gctgatacag ttgtagaccc aaaactcaca ccacctccaa gatcattata
     1741 agccgctaca cctgcagttc ctgcaataag attggcaata tttgtagtta catcgtaaat
     1801 actgaaagtt tcagtcgggt ctggactaat ataacccgga aacggtcccg gtcccacatt
     1861 agggttaaaa agaaccagac tggccgcaca tatttgagta gatgaaagat cgaaaacaaa
     1921 aaaatcatga aaaatttcat tgttctcggc acctgctata taattagtat tgcttggagt
     1981 gtgatcccct aagttatcat accaaccacg ctcagtagta ttaattggta caccggctga
     2041 tgctgtttgt acatttatgc ctaaaaacat taaaagcgta attgtaaaac atacttgtat
     2101 aaaaagtttc actattatat tcctccttct ctaactaaag ctatttactt gttcatatca
     2161 catatacact taaaatacaa gctatattac tcaagtaatt gcttaccgta atcttaagta
     2221 taatatttta ggggttaact cagtattgac actactctta aaattatagc ttctctactg
     2281 ccctgcactt atgctactta gacacatggc tgtagatcat ggggtttatt tatacaaata
     2341 atataataat ttacgtggaa ctgtagcaaa aactgtagca ataaattgaa tagaaatcag
     2401 aaaaagttat a
//
LOCUS       DBNGSV010000356         1971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_550292, whole genome shotgun sequence.
ACCESSION   DBNGSV010000356 DBNGSV010000000
VERSION     DBNGSV010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_550292"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..982
                     /locus_tag="AC159U_12720"
     CDS             269..982
                     /locus_tag="AC159U_12720"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3337959.1"
                     /translation="MKEQILEISLNGMVLDTKRLEKPEITIGRGSENDIVINNPAVSR
                     HHATIFTNVNTVVIVDSGSTNGTFVNNNRVMEKQLDVGDEIIIGKMIIRYKIKDVDHD
                     EDTVIDLAFQSEGEQESFPDGTFRVNEQDKQRILDKVKSDKISTIPTLIASNREFHIS
                     NNHFTIGKSTGNNLKVRGWFVKNLQANIVRLNPKTYKLISYGSFLAPTKVNGTSVSQK
                     ILDSGDVIEVGNVKVVYQH"
     gene            complement(1026..>1971)
                     /locus_tag="AC159U_12725"
     CDS             complement(1026..>1971)
                     /locus_tag="AC159U_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR3337960.1"
                     /translation="LDIFKSRQIEFARLNLSYTILSKRKLSELVKNKYVSGWDDPRMP
                     TISGLRRRGFTPDSIINFCDQIGVSKNNSVIDVSLLEYCVREDLNKKAKRAMAVLNPV
                     KLIIDNYPDDLEEELDAINNPEDESMGSRKVPFSKTLYIERDDFREESSKKFYRLSPG
                     KEVRLRYAYFVTCTDFKKDDKTGEITEVHCTYDPATKGGSSPDGRKVKGTIHWVSARH
                     CIEPEVRIYDRLFSVPDPDVAKDDNDYTDYLNTDSIQILKSCKLEPSLKEAKPFIYYQ
                     FERLGYFCVDPKEFENGNFIFNRTIGLRDSWAKIDKSK"
BASE COUNT          652 a          314 c          342 g          663 t
ORIGIN      
        1 taaagcgaat gctatctgtt gttctgtaaa tcttgacttc ctcatggcaa aatacctcct
       61 gatttattgc attattttgc cagatttctc tacttttacc tggttcagtt tgcagggtga
      121 aggtcagtta atataaaaaa tgtattgcat taatataata tatttgttag aatatgtgta
      181 aatctaaagt tttatataat gcaatttgat gtctaagact tagatagtat gtcaatgtag
      241 tataatatta aattttagga gcatgaatat gaaggaacaa atactagaaa tttcattaaa
      301 tggaatggtg ctcgatacca aaagactgga aaaacctgaa attacaatag gtaggggatc
      361 agagaatgat attgtaataa ataaccctgc agtatccaga catcatgcta ctatttttac
      421 taatgtaaat actgttgtga tagttgattc cggaagcacg aacggcacat ttgttaataa
      481 caacagagta atggaaaaac agctggatgt aggagatgaa attataatag ggaaaatgat
      541 aatcagatat aaaataaaag atgtggatca tgatgaagat acggttatag acctggcttt
      601 ccagagtgaa ggtgaacaag aatcatttcc tgatggaacg tttagagtta atgaacagga
      661 taagcaaaga atattagata aagttaaatc tgataaaatt agtacaatcc ctactcttat
      721 tgcaagtaat agagaatttc atattagtaa caatcacttt actataggaa agagcaccgg
      781 taataactta aaagtgagag gttggtttgt gaaaaatttg caagctaata ttgtcagatt
      841 gaatcctaaa acctataagc tgattagtta tggttctttc ctggctccta ccaaagttaa
      901 tggaacatct gtttctcaaa agatactgga tagcggagat gtaatcgaag taggaaatgt
      961 aaaggttgta taccaacact aatatatatc caatatcgaa gcagataagt attggatata
     1021 aatgattact ttgacttatc tatttttgcc cagctgtctc taagccctat ggttctatta
     1081 aaaataaaat taccattttc aaactcttta ggatcgacac aaaaatatcc aagcctttca
     1141 aactgataat atataaatgg cttggcttct tttaatgacg gttctaactt gcatgatttt
     1201 aatatttgta tcgagtcagt atttaaataa tctgtatagt cattgtcatc tttagctaca
     1261 tccggatcgg gtactgaaaa aagtctgtca tatattctga cttcaggttc aatacagtgc
     1321 cttgcagaaa cccagtgaat ggtccctttt acttttctgc catcaggtga tgagccgccc
     1381 tttgtggcag gatcgtaagt gcagtgaact tcggtaattt cgccagtttt atcatctttt
     1441 ttaaagtccg tacatgtaac aaaatatgca taacgaagtc ttacttcttt tccaggggat
     1501 agtctgtaaa actttttaga agattcttct ctaaaatcat ccctttctat ataaagtgtt
     1561 ttcgaaaatg gcactttcct gcttcccata gattcatctt ctggattatt aatagcatct
     1621 aattcttctt ctaaatcatc agggtagtta tcaattataa gctttacggg atttaatact
     1681 gccatagccc gcttagcctt tttgtttagg tcttctctaa cgcaatattc tagtagtgaa
     1741 acatcgataa cgctgttatt ttttgatacc ccaatctgat cacaaaaatt aataattgaa
     1801 tccggagtaa aacctcgtct tctaagaccg gaaatagttg gcatccgggg atcgtcccag
     1861 ccagatacat atttattttt taccagttca cttaattttc ttttacttag tattgtgtaa
     1921 ctaagattta gtcttgcaaa ttctatttgc ctggatttga aaatatcaag c
//
LOCUS       DBNGSV010000357         6325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1450190, whole genome shotgun sequence.
ACCESSION   DBNGSV010000357 DBNGSV010000000
VERSION     DBNGSV010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1450190"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..803)
                     /locus_tag="AC159U_12730"
     CDS             complement(204..803)
                     /locus_tag="AC159U_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HIR3337961.1"
                     /translation="MILLLNLTILVLLLSNTSFTASFGQDLINSAIERTKHEIRYDGS
                     YYSIDYPGGDVPENVGVCTDVIIRSYRKIGADLQKLVHEDMKANFSAYPSKKIWGLTR
                     PDKNIDHRRVPNLQTFFSRHGKVLKISNNPGDYKPGELVTWILPGNLPHVGIVTDKIS
                     STTGNPLIVHNIGSGPKLEDMLFDYKITGHYIYTHQNNN"
     gene            complement(925..1572)
                     /locus_tag="AC159U_12735"
     CDS             complement(925..1572)
                     /locus_tag="AC159U_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3337962.1"
                     /translation="MYIVELLKANEVEGCYHEKCPDLDELGIFHYEGKVSDRRLSQLL
                     IHTRKNVFFEANNRLFSMTKVLKQCFPDCRFIHLHRDPRDLFPSAFSKPIEITWNSGR
                     VRYNSVKLCGSPESSILERSCNYWTNYNQLILDDLEGEQYISLKFSDLIKGNIDSLEQ
                     FMNLKLSTQKIPPVNANKPVREEGKYEQFEKWNAEDQNMVINKCGPLMKTLGYEL"
     gene            complement(1846..2637)
                     /locus_tag="AC159U_12740"
     CDS             complement(1846..2637)
                     /locus_tag="AC159U_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3337963.1"
                     /translation="MAKSSELIVEHKDYVSIITINNPEKRNVVSPSILDGITNILAEL
                     EKADNTRCVVIRGQGNKAFSSGYDINAIGENDMIKNQDGSNYLPRCAKSIEDFPYPVI
                     AMINGHAFGAGLEIAVACDYRVCAEHAKLGMPPAKLGVLYSYAGIRKFLNLIGAGYTK
                     EMFLIGNPVDAIKAEKIGLVNSVKASGEIEEYTLNLAEEISQNAPMSMKTMKFMINKW
                     QGNQNLKNDQEESLIKMILEVQNSSDYKEGQKAFSEKRKPAFTGK"
     gene            2768..3274
                     /locus_tag="AC159U_12745"
     CDS             2768..3274
                     /locus_tag="AC159U_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337964.1"
                     /translation="MPLFEYECDTCMEKYDKDIDALASKLNKTNAEKIFKGNSGIVYI
                     EVTDGKRKKPKFTMGEKSDRRSLRRFKWTIDKANVLYMELEDFKFSELIYDKEEEKDL
                     KCPLCKKKKGVRRIFSTFKAIFDDKNKRAPRPGDDLKFHTDYKIMKDEERSSSWVGQD
                     HLNQYFQR"
     gene            3292..3774
                     /locus_tag="AC159U_12750"
     CDS             3292..3774
                     /locus_tag="AC159U_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3337965.1"
                     /translation="MPLYEFECIPCRDGIEKSLKQIQKKANNKILSDLVKKFDNIYRI
                     EVLDLEKQDLVAEYGKKNDDGSILDFYLDEGTKAVLFNSEEFRFTDLIYDESDEKKIK
                     CPVCNSKNYEKVFSSFAFTSDLSTDMPKPDMSGMPPEVAHRTMLTEYIEEKDRPKKNR
                     "
     gene            complement(3797..4459)
                     /gene="hisIE"
                     /locus_tag="AC159U_12755"
     CDS             complement(3797..4459)
                     /gene="hisIE"
                     /locus_tag="AC159U_12755"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3337966.1"
                     /translation="MSVDSLKFDDKGLIPIIVQNADDGQVLMFAWGKKETIEISLETK
                     KAHYWSRSRNKVWMKGEESGNTQKLLDAYYDCDKDVILYIVEQKGVACHTNEQSCFFT
                     KVDDTKQLSPSFKSSKSIKTIDDVYKVIEDRKANNKEGSYVSGLFEKGIDKILKKIGE
                     EAGEVIIGAKNADKDEIIYEVADLWFHSLIAMSYFDIKPQDIYIELDKRFGKTKESYG
                     RN"
     gene            4587..4796
                     /gene="rpoZ"
                     /locus_tag="AC159U_12760"
     CDS             4587..4796
                     /gene="rpoZ"
                     /locus_tag="AC159U_12760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3337967.1"
                     /translation="MARVTIEDCLEKVENRFALSITAMKRAKQLVKGAQALSTEKDNK
                     DVVTALREIAEEKVKVIYPEDPDRY"
     gene            4905..>6325
                     /locus_tag="AC159U_12765"
     CDS             4905..>6325
                     /locus_tag="AC159U_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3337968.1"
                     /translation="MRIGIDTGGTFTDFVAVDHGMIKVHKELSTPHDPSIAIINGIKK
                     LVDGNLEDVEIFHGTTVATNALLERKGSDVALVTTEGFEDIIEIGRQNRKELYNIFEK
                     QTDHLVKRTHRLGISERTDFKGKVVKPLSDTELLKLLRNIKKLKPEAVAISLINSYAG
                     AKNELIIEKALKELGIPISVSSKILSEFREYERTSTVVINAYLINKVLGYMTNLNEKL
                     GRSKISVLQSNGGLISTGQAGTEPVRILLSGPAGGVVGAFKLAENMGITKIMTYDMGG
                     TSTDVSLCDESIQFSNLNSVDNLPVNVAMIDLKTIGAGGGSIAYVDSGGALKVGPESA
                     GADPGPACYGKGTKPTVTDANVVLGRIDPKYFLGGEMSISKKRSIDALTRLSKKLKLD
                     VPTVAEGIVEVANANMEKALRVISLQRGHDPREFALFSFGGAGGLHACELACGLGISK
                     VIFPNNPGVLSAHGMLIADYFRD"
BASE COUNT         1964 a         1126 c         1248 g         1987 t
ORIGIN      
        1 aatatactta ttgctattac cggcaagata gataaaaagg tgtgtgctct aaaaaataac
       61 ttcatcagta catcctcctg gtatctggtc agattagacc aatatactat taatactttg
      121 gtttgtaatt gtaatgcgag tcgaaagaaa aaacaaggct tgatttttgg aatggagcaa
      181 ataagaatta ctctatattt tgatcagtta ttgttttgat gtgtatatat atagtggcct
      241 gtaattttgt aatcaaaaag catatcctcc agttttggcc cgcttccaat attgtgaacg
      301 attagggggt taccagttgt agaactgatc ttatctgtga ctatgccaac atggggcagg
      361 ttgcctggca gtatccaggt tacgagttcg ccaggtttgt agtcccccgg attgttggaa
      421 attttaagga ccttgccatg cctgctaaaa aaagtttgca ggtttggtac tcgtctgtga
      481 tcaatgtttt tgtcaggtct tgttaatccc caaattttct tagaagggta agcactaaaa
      541 ttggctttca tatcttcatg aactagcttt tgcagatcgg cgccgatttt acggtatgaa
      601 cgtataataa catctgtgca tactccgaca ttctccggta cgtcaccacc tggataatcg
      661 atggaatagt atgatccatc atagcgtatt tcatgttttg ttctttctat agccgaattt
      721 ataaggtctt gtccaaaact tgcggtaaaa gaagtgtttg agagaagcag tactaaaatg
      781 gtcaggttta gtagcaaaat catattttta ctaacttctg agttactgta gtgttacaca
      841 aaccggttat ttctccttca aatgtgagag gctctctcat aaaaatctct cttattttct
      901 aattggctgc taataagata aatatcacaa ctcataacca agagtcttca taagtggtcc
      961 gcatttattt ataaccatat tttgatcttc tgcattccat ttttcaaatt gttcatattt
     1021 accctcttct ctcacgggtt tatttgcatt aaccggcggg attttctgag ttgataattt
     1081 taggttcata aattgctcta atgagtcgat attgcctttg attaggtcag agaatttaag
     1141 agaaatatat tgttcgccct ctaagtcatc gagtattagc tggttataat tagtccagta
     1201 attacaagac cgctcgagta tggagctttc tggagatcca cacagtttaa cactgttata
     1261 gcgtacacgg ccgctgttcc aggtgatttc gattggcttt gaaaatgcag atggaaacag
     1321 gtccctgggg tctctgtgaa gatgtataaa ccggcagtcc gggaaacact gttttaaaac
     1381 ctttgtcatt gagaataggc gattatttgc ttcaaaaaaa acgttttttc ttgtatgtat
     1441 taataattgg ctgagtcttc tatcagatac tttgccttca taatgaaata ttcccagttc
     1501 atccaaatcg ggacatttct catggtaaca accttcaact tcgttagctt taaggagttc
     1561 tacgatgtac attgaaccac aactgccagc gttttggcag aagtagcgaa ggcctgaaac
     1621 tggctgtaaa ttaaccggta tgtagtaagg ccagaaatgc tggaagcttt ttgcttactt
     1681 gattaaatta aagatcgccg tgcgtaataa tgttcttaat tccataaaaa ttgatttatg
     1741 tatatgatcc gtaattttga agtggtctca ttataaacgt ttgtatagta atttaaaatt
     1801 cttatataaa tatcctcgtt aatgcttaaa taaagaagaa atagactatt tgcctgtaaa
     1861 agccggtttt cttttctctg agaatgcttt ctgtccttct ttataatccg agctgttttg
     1921 aacttcaagg atcattttta taaggctctc ttcctggtca ttctttaaat tctgatttcc
     1981 ctgccattta ttgatcatga atttcatggt tttcatagac attggtgcat tctgagatat
     2041 ttcttctgcc aggtttaatg tgtactcttc aatttcccca gaagctttaa cgctattaac
     2101 aagcccaatt ttctctgcct tgattgcatc caccgggttt cctattaaaa acatctcctt
     2161 tgtgtatcca gctccaatca ggtttagaaa ttttcttatc cccgcatagg aatataaaac
     2221 gcctagcttt gccggaggca tacccagttt tgcgtgttct gcacacactc tataatcaca
     2281 cgcaactgca atctcgagcc ctgcgccaaa agcatggccg tttatcatcg caatcacagg
     2341 gtaggggaaa tcttctatac tttttgcgca tcttggaaga tagttgctcc catcctgatt
     2401 tttaatcata tcattttcgc caatagcatt gatgtcataa ccggatgaaa atgccttgtt
     2461 gccctgtcct cttataacta cgcatctggt gttatccgct ttttccagtt cggcaagaat
     2521 gtttgttatt ccgtccagaa ttgatggaga gacaacgttt cttttttcag ggttattaat
     2581 tgtaattatc gaaacatagt ctttgtgttc aacaatcaat tcactgcttt ttgccaatat
     2641 tacagtcctc catctgggaa ttattaaaca atgatataat atattatgcg taaattaaca
     2701 ccttgactgg aataatatca tcattaggtt aaaataaaca gcttaaattc caggggagag
     2761 tataacaatg ccactttttg aatatgaatg cgatacatgc atggaaaaat atgacaagga
     2821 catagatgcc cttgcatcca aacttaataa aacgaatgca gagaaaatat tcaagggaaa
     2881 ttccggaatt gtgtatatag aagtcaccga cggcaagcgc aagaaaccca agtttaccat
     2941 gggtgagaaa agtgacagaa gaagccttag aagatttaaa tggactatag ataaagccaa
     3001 tgtgctttat atggagcttg aagactttaa attcagcgaa cttatctatg acaaggaaga
     3061 ggaaaaagat ttaaaatgtc cgctatgtaa gaagaaaaag ggagtgagaa gaatattttc
     3121 tacttttaaa gcaatatttg atgataaaaa caaacgtgct ccaagaccag gagatgattt
     3181 gaaatttcat acagactaca aaatcatgaa agatgaagaa cggtcaagca gctgggtagg
     3241 gcaggatcat ctaaatcaat attttcaaag ataaaaagga gagacaaaga tatgccgctt
     3301 tatgaatttg agtgtattcc atgtagagat ggaattgaga agtcattaaa acagatacag
     3361 aaaaaggcta ataacaagat tttatctgat cttgttaaga aatttgataa tatataccgt
     3421 atagaagtgc ttgaccttga aaaacaggac cttgttgcag aatatggcaa gaaaaacgat
     3481 gacggttcaa tattggactt ctatcttgat gagggcacaa aagcggtttt gttcaattcc
     3541 gaagagttcc gttttactga tcttatctat gatgagagtg atgaaaagaa aataaaatgt
     3601 cctgtttgta attcaaagaa ttatgaaaag gtattttcga gctttgcgtt tacaagcgat
     3661 ctgtccactg atatgccaaa gcccgatatg tcaggcatgc cgcctgaagt tgctcacaga
     3721 acaatgttaa cagagtatat tgaagaaaag gacagaccta aaaagaacag gtagctctta
     3781 tttgtatatg aagtagttag ttcctgccat aagattcttt ggttttacca aacctcttat
     3841 caagctctat gtagatatcc tgtggtttaa tatcaaaata agacatagca atcagagagt
     3901 ggaaccacag gtcggcaacc tcatagatta tctcatcctt atcggcgttt tttgccccga
     3961 ttatgacctc gccggcttct tcccctatct tttttagtat cttatcaatg cccttttcaa
     4021 aaagcccgct cacataggag ccttctttgt tattagcctt tctgtcctct ataactttat
     4081 atacgtcatc aatggtcttg atagatttag agcttttaaa gcttggggat agttgtttgg
     4141 tatcatcaac tttggtaaaa aagcagcttt gctcattagt gtgacaggct acaccctttt
     4201 gttctactat ataaaggatg acatccttat cgcaatcata gtatgcatcc aataatttct
     4261 gagtatttcc cgattcttca cccttcatcc atactttatt tcttgatctg ctccagtagt
     4321 gagctttttt ggtttccagg cttatttcaa tagtttcctt tttgccccag gcaaacatga
     4381 ggacctgtcc gtcgtctgca ttctgcacga taataggaat aaggccttta tcatcaaatt
     4441 ttagtgaatc tacgctcata attgtttatt atacagaaat taataagatt tttttaaata
     4501 ttaaaacaat caatacatta tagttgtata aatctgaata taaagtaaat taatcagtta
     4561 ataagaaatt acatcaggag tatctaatgg caagagtaac tattgaagat tgtttggaaa
     4621 aggttgaaaa cagatttgca ctatctatta ctgctatgaa aagagcaaag cagcttgtta
     4681 agggtgcgca ggcactatca actgaaaagg ataataaaga cgtagttaca gcacttaggg
     4741 aaattgctga agagaaagta aaagttattt accctgaaga ccccgatcgc tattgatcac
     4801 ggatgctatt gataattgac tgagaagttc ttatcgttaa ttaatatttc ccatttagta
     4861 tataccatac aagactatag ctaaaatata gttcatttcc ttaaatgaga attggaattg
     4921 acacaggcgg tacgttcact gattttgtag ccgtggatca cggcatgata aaagttcata
     4981 aggagctctc aactccacac gatccatcca ttgcgataat taacggcata aaaaaactgg
     5041 tggatggaaa cctcgaagat gttgaaatat ttcacggtac aactgttgct accaatgcac
     5101 tgcttgaaag aaagggctcg gatgttgctc ttgttaccac tgagggtttt gaagatatta
     5161 tagagattgg caggcagaac agaaaagaac tctacaatat ttttgaaaag caaacagatc
     5221 atcttgtaaa aagaactcac aggcttggga taagtgaaag gacggatttt aagggaaaag
     5281 tcgttaagcc tttaagcgat accgaacttt taaaactttt aagaaatatt aagaagttaa
     5341 aacccgaggc tgtagcaatt tcattaataa attcttatgc cggggcaaaa aatgaattga
     5401 taatagaaaa agccctaaaa gaattaggta ttcccataag cgtatcatct aaaattcttt
     5461 cagaattcag ggagtatgaa agaacatcca ctgttgttat aaatgcatat cttattaata
     5521 aagtcctggg gtatatgaca aatctaaatg agaagctagg gcgttccaag atatctgtgc
     5581 tgcagtctaa cgggggactt ataagcacag ggcaggctgg tacagagcct gtaaggatat
     5641 tgttgtcagg ccctgcaggt ggagtagtag gcgcatttaa actggctgag aacatgggga
     5701 taaccaaaat catgacttac gatatgggtg gcacgtcaac agatgtatct ctttgtgatg
     5761 agtccataca gttttccaat ctcaacagtg tagacaatct tccggtaaat gttgcaatga
     5821 ttgatcttaa gaccatagga gccggaggag gatctattgc ttatgttgat tcgggtggtg
     5881 ctctcaaggt aggtcctgag agtgcgggtg ctgatcccgg gcctgcctgt tatggaaaag
     5941 gaactaaacc aactgtgact gatgccaatg ttgtgcttgg aagaatagac cccaaatatt
     6001 tccttggcgg agaaatgagt atctcaaaaa agagatcaat agacgctctt acaagacttt
     6061 caaagaagct taaactcgat gtacctaccg tagcagaggg aatagtcgaa gttgcaaacg
     6121 ctaacatgga aaaagcactt agagtaatct cgctgcaaag aggacatgat ccaagagagt
     6181 ttgccctgtt ttcatttgga ggagcaggtg ggcttcatgc ctgtgagctt gcatgtgggc
     6241 ttggcatttc taaagtgata tttccaaata acccgggcgt gctttctgca catgggatgc
     6301 ttattgccga ttatttcaga gactt
//
LOCUS       DBNGSV010000358         3776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_411484, whole genome shotgun sequence.
ACCESSION   DBNGSV010000358 DBNGSV010000000
VERSION     DBNGSV010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_411484"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="AC159U_12770"
     CDS             <1..409
                     /locus_tag="AC159U_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337969.1"
                     /translation="NIRWRISPKQVELATDSSLIEEYLNKDYKIYSHKLKLVNRAKLS
                     NVKHYFYKDQLYKIQINKDLSSYENHIINQLIDKSGFAMNKNEIIETIKSGNKYKGKI
                     QNNVAMVYLSKDKSDKSQEVNLVLGYKPLLDEI"
     gene            complement(504..2405)
                     /locus_tag="AC159U_12775"
     CDS             complement(504..2405)
                     /locus_tag="AC159U_12775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR3337970.1"
                     /translation="MVVEDELIVADDIKTSLQKLGYDVVATVSSGEQSIAKAEQLKPD
                     LVLMDIILKKEMDGIEAADIIVKRFNIPVIFLTAHSDDATLKKAKVSEPYGYILKPFD
                     ERDLLTNIEIALYKHKMETRLKMSEQWFSTTLKSIGDAIIATDNKGNVKFMNGIAEDI
                     TGWTMEQSGGKNLNQIFKIINESTLEEIENPIEVVLRKGSAVSSNSHTLLISKNGNRV
                     PIDNSASPIKSENGDIQGVVMVFHDLTEKKKLQEKLMKKQKLESLGVLAGGIAHDFNN
                     ILTAVMGNISISMLSLNTDSVIFDNLSKAEKACKRASELSNQLITFSKGGSPLKKTIN
                     IAELLRDCVQLVLNTRDIKYTLDIKGESLKVQAQRSQLTQVISNILINSVESISTGGL
                     IKISLDTVDLDSDSEPAVKEKFIRISIEDNGTGIDKESIGKIFDPYFTTKNSGSGLGL
                     AIVNSVIEQHGGSIEVDSNPGGGTKFTIYLPASDQQLVTESPYSSKLMTGRGKILVMD
                     DEPQVRELTQNVLRQVGYEVQICSDGKEVIEKYVRAANSDSKFDLVILDLIVPQGMGG
                     IETVEKLLDFDPHVSVIAMTGYTTNNIKTEYLDYGFKGFTQKPYDIKELSELVFSLIS
                     AGQKEKQVR"
     gene            complement(2447..3202)
                     /locus_tag="AC159U_12780"
     CDS             complement(2447..3202)
                     /locus_tag="AC159U_12780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3337971.1"
                     /translation="MNIYNTTTINKNLWLSLALTPLMDNDNVVIGGLAVVEDITERKN
                     SEQNLRRSLDEKEILLKEIHHRVKNNLQIISSLLHLQSNNIKQTEEIGSDIFKESQNR
                     IRSMALIHEKLYSSENFGEVNFKDYLQTLSSSLIRSYRINTKVHIDAENIFLNIDKSV
                     TCGLIINELLTNSLKHAFDNSSEAQIHIKLIRQNAHHELIIRDNGTGLDENFDIENAN
                     TLGLKLVKRLTEQLEGSLDYKVDNGTEFRISFH"
     gene            complement(3189..>3776)
                     /locus_tag="AC159U_12785"
     CDS             complement(3189..>3776)
                     /locus_tag="AC159U_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3337972.1"
                     /translation="LKEDQLAVMKAYRGYPGWFIKKESEIPYPRGFTWKTIIDGKPIY
                     CPDIDKDNVIGPAGKQVGTKSYLSTPINDNNITVGTINVHSLQQDAFDEEDLKLLQIV
                     AGQIEIAINNAKQAEALRLSEQLYKALFDQAPVGVYIFDIDYKITNCNDRMVDIIGST
                     RDKIVGLDMKKLRDSVFMPSMEKALKGEICYHEYI"
BASE COUNT         1101 a          752 c          585 g         1338 t
ORIGIN      
        1 aaatatcagg tggagaattt cccccaaaca ggttgagctt gcaacagatt cttcattaat
       61 tgaagaatac cttaataagg attacaaaat ttattcacat aaactaaagc ttgtgaacag
      121 agcaaaactc tccaatgtga aacattactt ctataaagac cagctataca aaattcagat
      181 aaataaagac ctgtcatcct atgaaaatca cataatcaat caattaatcg ataaatccgg
      241 atttgctatg aacaaaaatg aaattattga aacaatcaaa agcggaaata aatataaagg
      301 taaaatccaa aacaatgtcg caatggtata tctaagcaaa gacaaatccg ataaatcgca
      361 agaagttaat ttggtgctgg gttataaacc gcttttggat gaaatttagg attttattca
      421 atagaaaata tcaagaaatt agtgcagttt tgagattaga acatttaaaa ctctatctta
      481 attgcagaat atcaagctaa taactaccta acttgttttt ctttttgacc tgcgcttatt
      541 agactaaata caagctcgct cagttccttt atatcgtatg gcttttgcgt aaatccctta
      601 aaaccataat ccaggtactc agtcttaata ttgtttgtag tatatccagt catggcaatt
      661 acacttacat gagggtcaaa atctaggagt ttttccacag tttctatccc acccataccc
      721 tgagggacaa tcagatcaag aatcaccagg tcaaatttac tatcgctgtt agctgctctt
      781 acatattttt ctataacttc tttaccatct gaacaaattt gtacttcgta tcctacctgt
      841 ctaagaacat tttgagtaag ttccctcacc tgcggttcat catccataac caatattttc
      901 ccccgaccgg tcattaactt ggagctataa ggactctcag ttactaactg ctgatctgag
      961 gctggaagat atatagtaaa tttggttccg cctcccggat ttgaatccac ttctatggac
     1021 ccgccgtgct gttctataac agaatttaca attgcaagtc caagcccgct tccactgttt
     1081 ttggttgtaa aatatggatc aaatattttg ccgatactct ctttatctat tcccgtacca
     1141 ttgtcctcaa tactgattct aataaatttc tcttttacag ccggctctga atctgaatcc
     1201 aggtctacag tgtccaaact gattttaatt aatccaccag tactaatgga ctcaacagaa
     1261 ttaataagaa tgttgcttat gacctgcgtt aattgacttc tttgtgcttg aactttcagt
     1321 gattcgccct taatatctag agtatattta atgtctcttg tatttaatac taactgcaca
     1381 caatctctta ataattcggc aatatttatg gttttcttta gtgggcttcc ccctttagag
     1441 aaagtaatga gctgatttga aagttcgctt gctctcttac atgctttttc ggcttttgac
     1501 agattgtcaa atattaccga gtcagtgtta agagataaca tagatattga aatgtttccc
     1561 attacggcag ttaatatatt attaaaatca tgagcaatcc cgcctgcaag tactcccagt
     1621 gattctagtt tttgtttttt cattaatttt tcttgtaact tctttttctc tgtaaggtca
     1681 tgaaaaacca tgacgacacc ctgaatatcg ccgttttctg atttaatagg ggatgcgctg
     1741 ttatcaattg gcaccctgtt tccattttta gagatcaaaa gtgtgtgact atttgaactt
     1801 acagctgatc cttttcttag aactacttct atgggatttt caatttcttc aagagtactt
     1861 tcgtttatga ttttaaatat ttgatttaga ttttttcctc ctgactgctc catggtccat
     1921 ccagtaatat cttctgcaat gccgttcata aatttaacat tccccttatt gtcggtagct
     1981 attattgcat caccaattga ttttagagtc gttgaaaacc actgttcact cattttgagc
     2041 ctggtttcca ttttatgttt atataacgct atttcgatgt tagtcagtaa atctctttca
     2101 tcaaacggct ttaatatata accatatggt tcacttactt ttgctttttt aagtgtagca
     2161 tcgtcagagt gtgctgtaag aaagataacc ggaatattaa aacgtttcac aattatatcg
     2221 gcagcttcta taccgtccat ttcctttttt agtattatat ccatcaacac aaggtcgggc
     2281 tttaattgct cagcctttgc aatcgattgc tccccggaac tcacggttgc aacaacatcg
     2341 tatcccagtt tttgaaggga ggttttaata tcatccgcta cgataagttc atcttcaacc
     2401 accattatct tcaagtatca gctgctcata gatttacccc tggttatcaa tggaaagata
     2461 tcctaaattc agtcccgtta tccactttat aatctaatga tccttcgagt tgttcggtta
     2521 acctctttac aagtttaagt cccagagtat ttgcattttc aatatcaaaa ttttcatcta
     2581 atcctgtacc attatccctt atgataagtt catggtgagc gttttgcctt ataagcttaa
     2641 tgtgtatttg agcttcagaa ctattatcaa atgcgtgctt tagtgaattt gttaatagtt
     2701 cattaataat aagtccgcac gtaacggatt tatctatatt taagaaaatg ttttcggcat
     2761 caatgtgcac ttttgtgttt atccgataag aacgaataag cgagctgcta agagtctgca
     2821 ggtagtcttt aaaattaacc tctccaaaat tttccgaact atagagcttt tcatgtataa
     2881 gcgccatgga tctaattctg ttttgacttt ccttaaatat atctgaacca atttcttcag
     2941 tttgcttaat attgttagac tgaagatgta ataaacttga tattatctgc aagttgtttt
     3001 taaccctgtg gtgtatttct tttaggagta tttccttttc atcaagagac cttctaaggt
     3061 tttgctcaga gtttttcctc tcagtaatat cctctactac tgcaagtccg cctattacaa
     3121 cattatcatt gtccataaga ggagttagcg ccagagacaa ccagagattc ttatttattg
     3181 tagttgtatt atatatattc atgataacaa atttcccctt ttagagcctt ttccatagaa
     3241 ggcatgaaca cagaatctct tagctttttc atatcgagtc caacgatctt gtcccgggtt
     3301 gatccgataa tatccaccat tctgtcatta caatttgtta ttttgtagtc tatatcaaat
     3361 atatatacac caacaggagc ctgatcaaaa agagctttat acagctgttc ggaaagcctc
     3421 agggcttcag cctgcttggc attgtttatg gctatttcaa tctggcctgc aacgatttgc
     3481 aggagcttta aatcctcttc atcaaatgca tcttgctgca aagaatgaac atttattgtt
     3541 ccgaccgtaa tattattatc atttataggc gtggataaat agctttttgt tcctacctgc
     3601 tttcctgcag gtccaattac attatcctta tcaatatcag gacagtatat gggttttcca
     3661 tctattatag ttttccatgt aaaacctctg gggtatggaa tttctgattc cttttttata
     3721 aaccaacccg gataacccct ataggctttc atcactgcaa gctgatcttc tttcag
//
LOCUS       DBNGSV010000359         4094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1312614, whole genome shotgun sequence.
ACCESSION   DBNGSV010000359 DBNGSV010000000
VERSION     DBNGSV010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1312614"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..278)
                     /locus_tag="AC159U_12790"
     tRNA            complement(206..278)
                     /locus_tag="AC159U_12790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Ala,seq:ggc)
     gene            374..1672
                     /locus_tag="AC159U_12795"
     CDS             374..1672
                     /locus_tag="AC159U_12795"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR3337973.1"
                     /translation="MNEVNVGIIGAGTVGCGVVSILLENNELIQKRSGVKLNIKKIAD
                     IDIKRKRPVKIPKRLFTTDSREVINDPNIPIIVELVGGTTIAKDFVLGAIKNKKHVVT
                     ANKALLAHHGAEIFSTAARNNVNVGFEASVGGAIPIIISTKESYVPNNIQSIYGIMNG
                     TCNYILSTMTEKSLDFDVVLKDAQEKGYAEADPSFDIDGIDAAHKLSILIMLSYGQFF
                     KFNKIHVEGIRNISKLDISYSGELGYVIKLLAIAKAKENGIEAGVYPALVKKGTQMAD
                     VSGAFNAIYMVGDALGPTMLYGMGAGMMPTGSAVVGDIVQIAKSIKNKADSTVPRFYE
                     DKKHSRLLPISDKIANYYLRFEVKDKPGVLGKITNVLGDNSISIESVIQKGNSPGGDV
                     QVVMMTHKAREHDLKNALSKINRFSFVNNKPSFIRIENLN"
     gene            1697..2788
                     /locus_tag="AC159U_12800"
     CDS             1697..2788
                     /locus_tag="AC159U_12800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3337974.1"
                     /translation="MKGKSPILENYYKDIFDNLDQALLIIDENLYVEEINDSAEEIFK
                     ISRNKARGNKTDYFLPAEIEQLALKALKDNCVVQGNDIECKLKGGNTITLHANSIPLQ
                     IGKRSSRNVILQIKDMSLNKMLIQKNIQEQTNSMFGNLILGLSHELKNPLSGIKGASQ
                     ILIGELETEENKKIANIIVKEIDRLTRMLDNFNQLELFSDEIFVHIDIHEILSDITIL
                     EEKCIKSKNISFIHDFDIAIPNISGDANSLKQAFINLIKNSIEAVNNKTGKISITTRW
                     NTVYRQRGEPGIYIDISDNGSGIPKDKMEKIFSPFFTTKKKGTGLGLFFSQQTINKHG
                     GHIHVKSDTEAGTTFTIFLPAAKKGGKSG"
     gene            2790..>4094
                     /locus_tag="AC159U_12805"
     CDS             2790..>4094
                     /locus_tag="AC159U_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3337975.1"
                     /translation="MLNKILVVDDEESIRWVLQTSLGKQGYNVTVAEDGNTALEFLSN
                     EEFSVVLLDLNLPDIDGFDVLKIINERKIRSSVIIITAQNSVNNAIQAMQLGAYDYFS
                     KPFDINEVNDLVSKGIENYKNSIDIDYSNKITSSDKQLSSYGIVGNSKAMTAIYKIIG
                     KVADKDITILVTGESGSGKELIARAIHQNSPRAKYNLVSVNIAAIPNELLESELFGYE
                     KGAFTGASLRKIGRFEEAHRGTLHLDEIGDMSADLQTKLLRVLEEKKLYRLGSEKPVD
                     VDARIIVSTNRNLDDEVNKGNFRKDLFYRLNAITIEVPPLRQRKDDINILIDHFQNKY
                     SKQLNVSKKVLSEKAKEIVNSYDWPGNVRELENTVKRILVLCPDKIISRESLLEAAPG
                     LLKDLSGKKGFDMAIENEVSNLINSYSGDLPNGIYENVINRVE"
BASE COUNT         1520 a          647 c          781 g         1146 t
ORIGIN      
        1 gaatcgtgaa gaattatgat ttagattaaa taattcatta ccttcaacaa taatctgctt
       61 aacttcatct ataaattctt tataactaaa ttttttatta aagaacataa ttattatatc
      121 aatattgaat atcaaccgta cccaaaaccg tacccagctc aattgaagga aatatggtag
      181 tgtcctaacc tactgtaatt attaatggag ccgatcggga tcgaaccgac gacctcatga
      241 atgccattca tgcgctctcc caactgagct acggccccta ctctgcttaa tttattctta
      301 tatttatttt atgtcaaata ttttgatgta taatcaaatt caataaaatt gggatgttac
      361 aaggagtttt ttaatgaatg aggttaatgt tggaataatt ggcgctggta cggtgggatg
      421 tggagttgtt tcgatcctgt tagagaataa tgagcttatt caaaaaagat ctggtgttaa
      481 attaaatatt aaaaaaattg ctgatattga tattaaaagg aaaagacctg taaagattcc
      541 aaaaagatta tttactaccg atagcaggga agttataaat gatccaaata ttcccataat
      601 agttgagctt gtgggcggaa caactattgc taaggacttt gtattaggtg ctattaaaaa
      661 taaaaaacac gtagttactg ccaacaaagc gctgcttgcc catcatggag ctgagatttt
      721 ttctacagct gcaagaaaca acgtaaatgt aggatttgaa gcaagcgtcg gtggagcaat
      781 ccccattatt atctcaacaa aagaaagcta cgtgccaaat aatattcaat caatctatgg
      841 aataatgaac ggtacctgca actatattct atctaccatg acagagaaaa gtcttgattt
      901 tgacgtagtg cttaaagatg ctcaggaaaa ggggtacgcg gaagctgacc cctcgtttga
      961 tattgacgga atagatgcag cacacaaact ctcaatacta attatgcttt catatggtca
     1021 gttttttaaa tttaacaaga ttcatgtaga gggaataaga aatatatcca agctcgatat
     1081 ttcatattca ggcgaacttg gttatgtaat taaacttctt gcaatcgcaa aagcaaaaga
     1141 aaacggtata gaagcaggag tgtatcccgc acttgtaaaa aagggaactc aaatggcaga
     1201 tgtatccgga gcatttaatg ctatatatat ggttggagat gcacttgggc ctaccatgct
     1261 ttatggtatg ggagccggga tgatgccaac cggaagtgct gttgtagggg atatagtaca
     1321 aattgcaaaa tcaattaaga ataaagcgga tagcactgta cccaggtttt atgaagataa
     1381 aaaacactca cgtctactgc caattagtga taaaattgca aattactacc ttagatttga
     1441 agtgaaggat aaacccggag tacttggcaa aataaccaat gtactaggtg ataatagtat
     1501 aagcatagaa tcggtaatcc aaaaaggaaa ttcacccgga ggagacgtac aggtagttat
     1561 gatgacacat aaagccaggg aacatgacct aaaaaacgct ctgagcaaaa ttaacaggtt
     1621 tagttttgtc aacaataaac ccagttttat caggattgaa aatttaaatt aatatttcca
     1681 ttatattcaa taatacatga aggggaaaag ccctattcta gaaaactatt ataaggatat
     1741 atttgataac cttgatcagg ccttgcttat aattgatgaa aacttatacg ttgaggaaat
     1801 aaatgacagc gccgaagaga ttttcaagat tagcagaaac aaggccaggg gtaataaaac
     1861 cgattatttt ctacctgcag aaattgaaca actagcgcta aaagcattaa aagataattg
     1921 cgtagtacag ggaaatgata ttgaatgcaa attaaaaggg ggtaatacca ttacactgca
     1981 tgcaaattca attccgcttc aaattggcaa aagatcttct agaaatgtca tcttgcagat
     2041 taaggacatg tctttaaata aaatgctcat tcaaaagaat atccaggaac aaacaaattc
     2101 gatgtttggc aaccttatct tgggattatc tcatgaatta aaaaatccgc taagtggcat
     2161 aaagggcgca tcacagatac tcataggtga gctggaaact gaagaaaata aaaaaatagc
     2221 aaatattatt gtaaaagaga tagacaggct tacaaggatg cttgataatt ttaatcagct
     2281 tgaactcttc tcggatgaaa tatttgtaca tatagatata catgaaattc tatcagacat
     2341 tacaattctt gaagaaaagt gtattaaatc taaaaacatt tcattcattc atgacttcga
     2401 tatagcaatt cccaatattt caggagatgc aaactcttta aaacaggctt ttataaatct
     2461 tattaaaaat tcaattgaag ctgtaaacaa taaaaccgga aaaatatcta ttaccacccg
     2521 gtggaacaca gtatacagac aaagaggtga acctggtatt tatatagata taagtgacaa
     2581 tggcagcggt attccaaaag acaaaatgga gaagattttt tcgccatttt ttactaccaa
     2641 aaaaaagggc acaggccttg gactcttttt ctctcaacaa acaataaata aacacggcgg
     2701 ccatattcat gtaaaatcag atacagaggc aggcacgaca tttactatat tcctgccagc
     2761 tgcaaaaaaa ggcggtaaga gtggctaaca tgttaaataa aattttagta gtcgatgatg
     2821 aagagagcat aagatgggta cttcaaacat cccttggtaa gcagggatat aacgtaactg
     2881 tagcggaaga tggaaatact gccttggaat tcttgtcaaa tgaggaattt tcagtagtac
     2941 ttttagacct taaccttccc gacatagatg gcttcgatgt tctaaaaata attaatgaaa
     3001 gaaaaattag atcatctgta ataataatta cagctcaaaa ctctgtaaac aatgctatac
     3061 aggctatgca gcttggcgct tatgattatt tttcaaaacc atttgatatc aatgaggtaa
     3121 atgatctagt aagtaagggg attgaaaatt ataaaaacag tatagatatt gattattcaa
     3181 ataaaattac cagctcagac aagcagcttt catcctatgg aatagtaggc aattcgaaag
     3241 caatgacagc aatctacaaa attattggca aggttgcaga taaggacatt actatacttg
     3301 ttaccggtga gagtggctcc ggcaaggaat taatcgcaag agctattcat caaaatagcc
     3361 caagagccaa atataatctg gtctctgtta atattgccgc tattccaaac gagttacttg
     3421 aaagtgagct ctttggttac gaaaaagggg catttacagg agcaagttta agaaagatcg
     3481 gaaggtttga agaagctcac aggggaacac tacacttaga cgaaattggt gacatgtctg
     3541 ccgatctgca gaccaaactg ctaagagtat tggaagagaa gaaactctat agacttggaa
     3601 gtgagaaacc agttgatgtt gacgctagaa ttatagtttc aacaaacaga aacctggatg
     3661 atgaagttaa taagggaaat tttagaaaag acctgtttta cagattaaac gcaatcacga
     3721 ttgaagttcc gcctttaaga cagcgtaagg atgacataaa cattttgata gatcattttc
     3781 aaaataaata ttcaaagcaa ttaaatgtta gcaaaaaagt attatcagaa aaagccaaag
     3841 aaattgttaa tagttatgac tggcctggca atgtaaggga gctggaaaat acagtaaaaa
     3901 gaatactggt tctttgccct gataaaatta ttagccgtga atcactttta gaagcagctc
     3961 cgggattatt aaaagatttg tcaggtaaaa aaggttttga tatggcaatt gaaaacgagg
     4021 tttcgaattt aataaattct tacagcggtg acttaccaaa cggtatctat gaaaatgtaa
     4081 tcaacagggt agaa
//
LOCUS       DBNGSV010000360         3038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_273382, whole genome shotgun sequence.
ACCESSION   DBNGSV010000360 DBNGSV010000000
VERSION     DBNGSV010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_273382"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /locus_tag="AC159U_12810"
     CDS             complement(<1..1893)
                     /locus_tag="AC159U_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3337976.1"
                     /translation="MYHNRKTLLPFICVFFSFLVVFVYKTHKDVKDIPDSFQQILSAS
                     SKLQITDKHGNPLNVTYQNRWNVHDVIELHDIPQFLKTAFIFSEDKRFYEHGGVDWLA
                     RTSTLYSNLKNLRAVRGASTITEQTVRMINTRPRTLWSRWIEGFEAAALERQFDKDEI
                     LEFYLNQVPYASNRRGIVQAARFYFDRDIDTLSNKEMLTLVVLVRAPTRMDLHNNPGD
                     SVQLSISALSERLYKKGVLSEIQRSSLQEQKFEIQKPAMDVYAPHFVQYALGQEELSY
                     NRYKRIVTTIDGSLQKKVQSLSDEHLVNLKAKNVLNSAVLVVNHLTGEVLSWVVGRSQ
                     NANAPGIFINAVTTPRQPGSALKPFLYALALEKGWSAATIIDDTPLSESVGHGLHSYN
                     NYSRSFYGPVTLRQALANSLNIPALKTLRYVGAEQYLRVLADLGFSELADHSNYYGDG
                     ISLGNGEVSLFHLVQAYSALANKGILRPLRVFHNSPAAQNSTRVFSEQVSSIIGTILS
                     DAEARALEFGQNSVLNFPVETAVKTGTSSDYRDAWAVGYNYKYTVGVWMGNLDQQPTE
                     GLTGSTGPTLLLRTIFSELTRNQQTKPLYLSPKLKRIDFCTNDRRLDPEDKNCVSQTE
                     WFVSRNT"
     gene            complement(1895..>3038)
                     /locus_tag="AC159U_12815"
     CDS             complement(1895..>3038)
                     /locus_tag="AC159U_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3337977.1"
                     /translation="VTLNDLERFRPHVQYMSLFGKSHYLQAALKTAQSEEIVDEVTKT
                     IISHSVQSAGKFSFNEELDDSYLRILASPLRANCSVLSSITKLEKSEKALELINDIPF
                     KLVRFITQTRGNRDHWENTQENVFCMNSLIDYSRLYEKTKPDFVANVSLDEQVMGSAK
                     FSDLRDDIKTVKRQITSDDPGKNKTVVINKTGEGRLYYSTRLRYAPLLENSSRVNAGI
                     DIRKEYSVERDGGWVLLDDPSKINRGELVRVDIFLSLPTARNFVVVDDPIPGGLEPVN
                     SDLATTSIDDADKGQFKASGGSWWFQYDDWYHYNVSRWSFYHKELRHDSAVFYSEYLP
                     AGNYLLSYTAQAIAEGNFTKMPIHAEEMYDPDVFGKGLPGLLEVAN"
BASE COUNT          865 a          694 c          561 g          918 t
ORIGIN      
        1 agtattacgg cttacaaacc actcggtttg agatacacaa ttcttatcct ccggatcaag
       61 tctcctatca tttgtacaaa aatcaattct ctttagtttc gggcttagat aaagaggttt
      121 tgtctgctgg ttcctggtaa gttctgaaaa aatcgttcta agtaaaagcg tgggaccggt
      181 tgatcctgta agcccctccg tgggttgctg atccagatta cccatccaca ctcctacggt
      241 atatttatag ttataaccta cagcccaggc gtcccggtag tcacttgaag ttccggtttt
      301 aacagcagtt tccaccggaa aattcaagac actgttttga ccaaattcga gcgccctggc
      361 ttcagcgtct gacaatatgg ttccaataat agatgacacc tgttctgaaa atactcttgt
      421 tgaattttgt gcagctgggc tgttatgaaa cactctaagc ggccttaata tacccttatt
      481 tgcaagtgcc gaataagcct gtacaaggtg aaacagtgat acctctccat taccaagaga
      541 tattccatca ccataataat ttgaatgatc cgcaagttca gaaaagccta aatctgctaa
      601 aacacgaagg tattgctcag ccccaacata ccgaagagtt tttagtgccg gaatattaag
      661 agagttggca agagcctgtc ttagggttac gggaccgtaa aatgatctgc tgtagttatt
      721 ataactatga agcccatgcc caacggattc tgacagagga gtatcatcta ttatagttgc
      781 agcactccac cctttttcca gtgctaaagc atacagaaaa ggttttagtg ccgagcctgg
      841 ctgccttggt gtggtaaccg catttataaa gattccgggt gcatttgcgt tctgacttct
      901 gccaacaacc catgataaaa cttcccccgt caaatgattt actaccagca ctgcactatt
      961 aagtacattt tttgccttaa gattaacaag atgctcatca gacaaggact gaactttttt
     1021 ttgtaaagaa ccatctatag ttgtaacaat tcttttatac ctgttatatg ataattcctc
     1081 ctgccccagt gcgtactgca caaaatgagg agcatataca tccattgccg gtttttgtat
     1141 ctcaaacttt tgctcttgca gactactcct ttgaatttca gacaatactc cttttttata
     1201 aagcctctcg ctaagagctg atatagataa ctgcacggaa tctccagggt tattatgaag
     1261 atccattcgg gttggcgccc ttaccaatac gacaagtgtg agcatctctt tattgctgag
     1321 cgtatcaatg tcacgatcaa aataaaacct tgcagcctgt actattcccc ttctgtttga
     1381 tgcataggga acctgattta aataaaactc caaaatttca tccttatcaa actgcctctc
     1441 caatgccgct gcctcaaacc cttcgatcca cctcgaccat aaagtgcggg gcctggtatt
     1501 tatcattcgc accgtctgct cggtaattgt gctggcccca cggactgccc ttaaattttt
     1561 taaattgcta tagagggtag atgtccttgc aagccagtca accccgccat gctcgtagaa
     1621 tcgcttatcc tctgaaaata taaatgccgt ttttagaaat tgcggaatat catgaagctc
     1681 tattacatca tgtacgttcc atcggttttg atatgtaaca ttaaggggat tgccgtgctt
     1741 atcggtaatc tgcagtttag acgaagcaga aagtatttgc tgaaaggagt caggaatatc
     1801 cttaacatct ttatgagttt tgtaaacaaa aactactaag aaagaaaaga aaacacatat
     1861 aaaagggagc aaagttttcc tattatgata cattttagtt tgcaacctcc agtaagcctg
     1921 gcagcccttt tccgaataca tccggatcat acatctcttc agcatgtatc ggcatctttg
     1981 taaaatttcc ttcagctatt gcctgagctg tatatgaaag gagatagttt cccgcaggca
     2041 ggtactcgga gtaaaataca gcagaatcgt gtcttagttc tttgtgataa aaactccagc
     2101 gtgacacatt ataatgatac cagtcatcat actgaaacca ccacgaacct cccgatgcct
     2161 taaactgtcc tttatcagca tcatcgattg atgtagtagc cagatcgctg tttacaggtt
     2221 ccagaccacc tgggattgga tcatctacaa ctacgaaatt tcttgcggta ggaagagaca
     2281 gaaatatatc taccctcaca agctcacctc tattaatctt tgacggatca tcgagcagta
     2341 cccagccgcc atcgcgctca acactatact cctttcgtat atcaatccct gcattaactc
     2401 tcgatgagtt ttccaaaaga ggtgcatagc gcagcctcgt ggagtagtag agcctgcctt
     2461 ctcctgtctt gtttatcaca acagttttat tcttacccgg gtcatctgag gtaatctgcc
     2521 tttttacagt tttgatatca tccctcaaat ctgaaaactt ggcactaccc attacctgct
     2581 catcgagtga tacatttgca acaaaatcag gcttagtttt ttcatatagc ctcgagtagt
     2641 caataagaga attcatgcag aacacatttt cctgagtatt ttcccagtga tctctattcc
     2701 ctcgggtttg agtaataaac cgcaccagtt tgaaaggaat atcatttata agctctaaag
     2761 ccttttcact tttctctaat ttagtaatgc tggatagaac tgaacagttt gctctaagcg
     2821 ggcttgcaag aattcgcaag taactgtcat ccagttcttc attaaaggaa aattttcccg
     2881 cagactggac cgaatgagat attattgttt tagtaacttc atctacaatt tcctctgatt
     2941 gcgcagtttt taaagcagcc tgcagataat gtgattttcc aaaaaggctc atatactgaa
     3001 catgcgggcg gaatctctct aaatcattta gggttacc
//
LOCUS       DBNGSV010000361         3808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1243064, whole genome shotgun sequence.
ACCESSION   DBNGSV010000361 DBNGSV010000000
VERSION     DBNGSV010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1243064"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1421)
                     /locus_tag="AC159U_12820"
                     /pseudo
     CDS             complement(168..1421)
                     /locus_tag="AC159U_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(1645..2400)
                     /locus_tag="AC159U_12825"
     CDS             complement(1645..2400)
                     /locus_tag="AC159U_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3337978.1"
                     /translation="MSGAEVQVSWVFAFLAGVVSFISPCVLPLIPGYISLVTKLSFDE
                     LTEKKASNTLKKVLIPSIFFVLGFSFLFVTLGASATFLGGFLQQNKFLLLKISGVVII
                     IFSLFVLELIKIPQLYGEKRLEIKDSNLNYLGPFLLGIAFGFGWTPCVGPILASILLY
                     ASTAEGASEGAILLFIYSLGLGLPFIITGLALTKALSAFSWIKRNYGIYKYVTGGALM
                     IIGILMVTNTLFYLNIYGQKILDSLGLDFWKSF"
     gene            complement(2397..2942)
                     /locus_tag="AC159U_12830"
     CDS             complement(2397..2942)
                     /locus_tag="AC159U_12830"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3337979.1"
                     /translation="MNRYFQITMLLCAFFIFACEQKVITSEETPQNTETQEKEELKTA
                     YNFKLENLDGSGTVELSDFKDKPVIVNFWASWCAPCREEMPFLQKTWEEHKDKGLVFI
                     GIDVIDDTASAKEFLDSFNINYLNLNDSKGKTSSKYSVIALPATFFINRKGKIVKQNY
                     GPFLGESGEKLFNKYLEEILN"
     gene            complement(3064..3450)
                     /locus_tag="AC159U_12835"
     CDS             complement(3064..3450)
                     /locus_tag="AC159U_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337980.1"
                     /translation="MRFFSIFSVFAIGAVMFISSATAGDMKMNESTDISVTGRVVDPA
                     CYIKAGMKGADHRVCAQACAKAGQAFGILDETNGVLYQVLATGMMKDPNEGLMDHAEE
                     IVTVEGKLFEKDGMKGIVPTKVKKGS"
     gene            complement(3563..>3808)
                     /locus_tag="AC159U_12840"
     CDS             complement(3563..>3808)
                     /locus_tag="AC159U_12840"
                     /inference="COORDINATES: protein motif:HMM:NF021365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3337981.1"
                     /translation="DTFNLLNNHDLVLGPATDGGYYLIGLCKISIELFNNIEWSSDKV
                     LKKTLNIANKLNFNYKLLDFRADIDTIADLNNHKYLF"
BASE COUNT         1189 a          732 c          594 g         1293 t
ORIGIN      
        1 attacagcag agtcgatact gcgctgcttg aagctaaaag aaggggtaaa ggtcagattg
       61 tatatgcttc aaaacttcct cacactgtag atgatatgta attccagagt gcaagaatta
      121 catatgactg ttgtttagtc atcttctaag agctgaatta ctttttgctc aagtctttgt
      181 ttgtatccgg acaaatcaaa ttcctttctt gcaactcctg attttattgc agcttctgct
      241 acagcataag actcgaatag aaggactctt gggtcaaatg gtttggggac tatgtatttg
      301 gggccaaatt caaaatggga tttgccatat gcctttgcaa cagattcatc ggcacccttt
      361 tttgcaagat cagcaagcgc atgagcagct gctactttca tttcttcatt aatggttttt
      421 gctctaacgt ctagagctcc tctgaagata aatggaaaac caagcacatt attaacctgg
      481 ttaggataat ccgatctacc tgttgcaatt ataatgtcct gtcttgcatc catagcatct
      541 tcataggtta tttcgggatc aggattagcc attgcaaaga ttattggatt gtcagccata
      601 gactttacca tttccttgct aactctgccg ccaacagaga gtccaacgaa tacatctgca
      661 cctttcattg catcttctaa ggtacgcaaa ttcgtctcta ccataaaacg ttctttttgt
      721 tcattaattc cttcttctct gcctttatat ataacacccc tgctatcaca caatatcatg
      781 ttttctcttc tggcgcccat ttgtataaaa aactcgccac aagcaatagc agaagctcca
      841 gctccattta tagtaattcg tacgtctgca atatctttat tgcctatttc acaggcattt
      901 atcagacctg cacctgatat tatagcggtg ccatgctgat catcgtggaa gacaggaata
      961 ttcattattt ttttaagttc ttcttcgata taaaaacatt ctggagcctt tatatcttct
     1021 agattaattc ccccaaatgt gggctcaagc agttttactg tttttataat ttcgtctggg
     1081 tcttcggtat ttagttctaa atcaaatgca tcaatatcag caaatttttt aaatagtacg
     1141 cttttacctt ccattacggg tttgccggca agagcaccta tattaccaag accaagaact
     1201 gccgtcccgt tactaataac agctacaaga tttcctctgt ttgtgtagtc ataaacaagg
     1261 tctggagtct gttctatctc tctgcatggc tctgcaacac cgggagaata tgccagtgat
     1321 aagtcccact gagtatcgca tggtttggtc gtatgtattc cggttttacc ttttgggtca
     1381 gaactatgat actgtagcgc tgcttccttc attttatcca tttactaata tatcccagaa
     1441 ttaatatatc ttttaatact gtattaaaat aaaagctata tagttacatg attaatgcat
     1501 tagtttcaat tatttttaat aaaaaaattc aacagctgaa atatttgttg ttcattagaa
     1561 taacatataa tagaattaaa acattagagg gagttaagac tataacttga agtaactaat
     1621 taaatattca ttatttttat tctattaaaa ggatttccaa aaatcgagtc caagagagtc
     1681 tagtatcttt tggccataaa tatttaggta aaaaagtgta tttgtgacca ttaagattcc
     1741 aataatcatc aaggcgcccc cagtaacata tttatatatt ccatagtttc tttttatcca
     1801 agaaaatgca gataatgcct ttgtaagagc aagacccgta atgataaaag gaagccctaa
     1861 acccaaggaa tatataaaca acaatatggc accctctgag gctccctctg cagtgcttgc
     1921 ataaagcaaa attgaagcaa gaataggacc tacgcacggt gtccatccaa aaccaaaggc
     1981 aatcccaaga agaaatggcc ctaagtagtt taaattacta tcttttattt ctaaacgctt
     2041 ttcaccgtaa agttgtggta ttttaattag ttccagcacg aaaagagaga atatgataat
     2101 tactacacct gatattttga gtagaaggaa cttattttgt tgtagaaaac cacctaaaaa
     2161 agttgctgaa gcgccaagtg tcacaaaaag aaaggaaaat ccaagtacaa aaaatatact
     2221 tggaattaat acttttttta gtgtattaga agcttttttc tcagtaagct cgtcaaacga
     2281 taatttagtt acaagtgata tgtatcctgg gattaatggt aaaacacatg gagagatgaa
     2341 tgatactact cctgccaaga aagcaaatac ccagcttacc tgtacttcgg ctccgctcaa
     2401 ttaagtattt cctctaaata tttgttaaat aatttttctc cgctctcacc taagaatgga
     2461 ccgtaatttt gttttacaat cttccctttt ctattaataa agaatgtcgc aggaagagca
     2521 attacactat acttgcttga tgttttacct ttgctgtcgt ttaagtttaa ataattaata
     2581 ttaaaagaat ctaaaaattc tttagcgctt gccgtatcat ctatcacatc tattccaata
     2641 aaaacaagcc ctttgtcctt atgttcttcc caggtttttt gtaaaaacgg catctcttct
     2701 ctgcatggtg cacaccatga agcccagaaa ttcactatca caggtttatc tttaaaatca
     2761 cttaactcaa cagtgcctga cccatccaaa ttttctaatt tgaaattata ggctgttttt
     2821 aattcttctt tctcttgtgt ttcagtattc tgaggggttt cttcagatgt gatcaccttt
     2881 tgttcacacg caaaaataaa aaaagcacat agaagcattg taatttggaa gtatctattc
     2941 atattttcat aaaattaatc agtatttttg aatttaaatc agtaaatatg ataagtataa
     3001 attattagat agcaagaaaa aggaaaagga agcatgattt ttcatgcttc cttaaaatat
     3061 agattaactt ccttttttta ccttggttgg aacgatacct ttcataccgt ctttttcgaa
     3121 aagtttacct tcaactgtta caatttcttc tgcatgatcc ataagtcctt cgttaggatc
     3181 tttcatcatt cctgttgcta aaacctggta gagaacgccg tttgtctcat ccagtattcc
     3241 aaaagcctgg cctgccttgg cacatgcctg agcacatact ctgtgatcag cgcctttcat
     3301 gcctgccttt atgtaacaag cagggtctac tactcttccg gtaacagaaa tgtctgtgga
     3361 ttcattcatt ttcatatcac ctgcagttgc tgatgatata aacattactg cacctattgc
     3421 aaaaacacta aatatactaa aaaatctcat atattttcac ctcctcatat agagttatta
     3481 cgtttttgat aattaatatt catattaact atcaatctaa taactaggat gcatataata
     3541 gaggaaggtt tcaggattgt atctaaaaca gatatttatg gttgtttaaa tctgcaattg
     3601 tatcaatatc tgctctgaaa tctagcaatt tgtagttaaa attgagctta tttgcaatgt
     3661 ttagagtttt tttaagtact ttatcactac tccattcaat attattaaat aattctatgc
     3721 tgattttaca aagtccaatt agataatatc caccatcagt agccggacct aatactaaat
     3781 cgtgattatt cagcaaatta aaagtgtc
//
LOCUS       DBNGSV010000362         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_897815, whole genome shotgun sequence.
ACCESSION   DBNGSV010000362 DBNGSV010000000
VERSION     DBNGSV010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_897815"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..988)
                     /gene="istB"
                     /locus_tag="AC159U_12845"
     CDS             complement(215..988)
                     /gene="istB"
                     /locus_tag="AC159U_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR3337982.1"
                     /translation="MSETLIMERIEANLSRLRMPRVREILLSYLKAAEEHEKSYLSFL
                     DELLEEEVAQKEQRRVETVLRVSGLPFIKTIDDFDFTFHPRLDRQRVLALFDLTFIKQ
                     KENVVFLGPPGVGKTHLAVALAVKACQAGLSIYFTTMGDLVGKLKKDHESNRSHRGRS
                     YYKSGLVVVDEVGYTPISREECNLFYHFISTRYEKTSTIITSNKAFSEWTELFHDPII
                     ITAILDRLLHHSVVINIKGNSYRLKGKLPMEQKEVKSDG"
     gene            complement(988..>2055)
                     /gene="istA"
                     /locus_tag="AC159U_12850"
     CDS             complement(988..>2055)
                     /gene="istA"
                     /locus_tag="AC159U_12850"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR3337983.1"
                     /translation="EEDGQYKGTWIYDHLQPLGYTGSYEMVKRKVRQLKEEMGRKAYI
                     RFETEPGRQAQVDFGEFKVQRDDGGVEKYYLFSMILGYSRKLYAELIAKCDLPNFLDC
                     HIRAFEYFGGVPQEILYDRMKNVFIRKVAGKAEFNKTLMGFAVHYGFKPEVAPSYAPW
                     VKGKVERPYHFIREGFWRGYSFINPKATNHDLLAWLQIKDERIHGTTHEKITVRFERE
                     KPHLGELPLYAFDTSYRAYRKVGKDCTVHFEGNRYVVPHRYVGKKVVLRVKEKKLRIL
                     ADEKLVVSYVTPEGKGHLVQDPRFYEDLKKDREMNRRKYSHGRISKGRAKQTISPTRM
                     LYEMDVDVRPLETYTRFVEGM"
BASE COUNT          503 a          565 c          376 g          611 t
ORIGIN      
        1 caactccggc gttaccctcc cgctaaacag ctcgcaactg ttattatcca gtcaagtacc
       61 ggactttgac ttagagtttg tagtatgtac tctgcaagca agtgagtgga atgcatattt
      121 tttgcagaat gtgtaacttt ttaaggataa agtagagcta ctgtcaagga aaatttaaaa
      181 gtgtaccaaa atgggtcatc gaaaagtgta ccactcaccc atctgattta acctcttttt
      241 gttccatggg tagttttcct tttaaccgat aactatttcc cttgatgttt atcaccacac
      301 tatggtggag cagtctgtcc agtatggcgg tgatgatgat cgggtcatga aaaagttctg
      361 tccactcaga aaatgccttg ttagaagtaa tgattgtgct ggtcttctca taccgggttg
      421 agataaaatg atagaacaga ttgcattcct cacgactgat gggtgtatag cctacctcat
      481 ccacaactac cagcccagat ttataataac tccttccccg atgacttcta tttgattcat
      541 gatccttctt cagtttacca acaagatctc ccatggtagt aaaatagata cttaaccccg
      601 cctggcatgc tttcaccgct aacgccaccg caagatgggt cttgcccaca ccgggaggtc
      661 ccagaaaaac cacattctct ttctgtttaa tgaatgtcag atcaaaaagt gcaagcaccc
      721 tctgccgatc cagccgggga tggaaggtga agtcaaaatc atcaatcgtt ttaataaagg
      781 gtagtcctga taccctcagc actgtttcta cccgcctctg ctccttctga gcaacttcct
      841 cttccaggag ttcatccagg aaacttaagt agctcttttc atgctcctct gctgccttca
      901 ggtaagataa cagtatctcc cgtactctcg gcattctcaa ccggcttaag tttgcctcta
      961 tccgctccat gatcagtgtc tcactcatca catcccctcc acaaacctgg tgtatgtttc
     1021 cagaggtctt acatcaacat ccatctcgta gagcattcgt gttgggctaa tggtctgctt
     1081 tgctcgcccc ttacttatcc ttccatggct gtatttacgt ctgttcattt cgcgatcctt
     1141 cttgagatct tcgtagaacc gtgggtcttg taccagatgc cctttccctt caggtgtcac
     1201 atagctcacg acgagctttt cgtctgcaag gattctcagt ttcttctcct tgacccgtag
     1261 caccaccttc tttcccacat aacggtgagg caccacatac cgattcccct caaagtgcac
     1321 ggtacagtcc tttcccacct tccggtatgc acggtaggat gtgtcaaatg catacaatgg
     1381 caattctccc agatggggct tctcacgttc aaaacgtacg gtgatcttct catgggtcgt
     1441 cccgtggata cgctcatcct ttatctgcaa ccatgctaac agatcgtgat tggttgcctt
     1501 ggggtttata aaactgtatc cccgccagaa tccctctctg ataaaatgat aaggcctttc
     1561 tactttcccc ttcacccatg gtgcatagga tggcgctacc tcaggcttaa aaccataatg
     1621 aacggcaaat cccatcaggg tcttattaaa ctctgccttc ccggctacct ttcttataaa
     1681 tacattcttc atccggtcat ataatatctc ctgcggtact cccccaaagt attcaaatgc
     1741 cctgatatga cagtcaagaa agtttggaag atcacacttc gctatcagct cggcatataa
     1801 cttcctcgaa taacccagaa tcattgagaa caggtagtac ttctccacac caccatcatc
     1861 tctttggacc ttaaactccc caaaatctac ctgtgcctgt ctgccgggct cagtttcaaa
     1921 ccggatgtat gccttcctac ccatctcttc cttcagctgc ctcaccttcc gctttaccat
     1981 ctcatagctg ccagtatatc ccagaggttg tagatgatca tatatccagg tccctttata
     2041 ctgcccgtct tcttc
//
LOCUS       DBNGSV010000363        11763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_481680, whole genome shotgun sequence.
ACCESSION   DBNGSV010000363 DBNGSV010000000
VERSION     DBNGSV010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_481680"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..657)
                     /locus_tag="AC159U_12855"
     CDS             complement(145..657)
                     /locus_tag="AC159U_12855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3337984.1"
                     /translation="MPKSLILYRHAKSDWGAESNSDHERPLNERGIDSAEIMGKVLAL
                     ANQVPELVITSTAVRAKETLILSVESGEWKCDVEEDSRIYHEDTSTVIEIIKSVSNKY
                     DDIMLVGHEPKWSMLASTFIGGGQINFPTACMAKIEFDINNWEFVDARQGILKWLLQP
                     SFFRKGKFDF"
     gene            754..996
                     /locus_tag="AC159U_12860"
     CDS             754..996
                     /locus_tag="AC159U_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337985.1"
                     /translation="MSVMSIRIDDKKRKALKMIAAIEGKNMSTIVGDLIDQYILNNKK
                     KIRNTLEQEQLIHLMELSETSFSEWDNKEDEIYDEL"
     gene            986..1318
                     /locus_tag="AC159U_12865"
     CDS             986..1318
                     /locus_tag="AC159U_12865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3337986.1"
                     /translation="MNYKKWDIVLVPFPFTDLKTTKKRPALVISPDNYNNGYDLVILF
                     ITSQMNSPKRLGDYYISEWKKWGLPKPSMIRMKFATIHKSIVIKTLGKLNNKDTSGLS
                     KNLIEFLT"
     gene            1331..2257
                     /locus_tag="AC159U_12870"
     CDS             1331..2257
                     /locus_tag="AC159U_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337987.1"
                     /translation="MMKPKKILLISLIVLLILWIGGNLGLYLFAKHFLIGEDRLELFN
                     NVPEIIEIKYTETDVEKIYFGDIEFILAPRGYDLVDIYPGVYIQEREVEKYSVHSTLM
                     FKYANERKNITIYFNNTDQKLVDDYIEFSNYTLNDFSLWNTLYNYNSFYKLAAKWILL
                     PSNNDLEKYPLYIVNSQYFKGFWKVSSGKKSSLLSFDFSTKENSYSIMLPGSDRDLIT
                     DNMKNIISSIKPFENKEEVTSELKSKYKNETAIFEKELALISLITIEGPTVEYLSDLK
                     ELLIEKSAREENIGDIQEQIDILKSKLDKVTN"
     gene            complement(2327..3013)
                     /locus_tag="AC159U_12875"
     CDS             complement(2327..3013)
                     /locus_tag="AC159U_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337988.1"
                     /translation="MFKKIILLILISYICIGVLYAADIESKFGSLNEVDGKPHDLAWS
                     YAEAFKLNINLPFSKKSYAVLKVLNDSNNIYFLFKFKTPKDIVGQSFFIRFNDKNGKF
                     IYDGQDVIGMNPVHKPNYLIDSVRTSQPPCSMPDGKYYEGKCGFEDTKVGGTRDGLGT
                     YSTDNNYSYYEFSHPLASKDKDVQLRPGQIFGIILDLRLIDKSGEYPESYGDTDFPES
                     GYLSVKLAAN"
     gene            complement(3039..3764)
                     /locus_tag="AC159U_12880"
     CDS             complement(3039..3764)
                     /locus_tag="AC159U_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337989.1"
                     /translation="MMINRKYFICIIFLLITTLLSGCVYYRLLKLKRQFANFEENFEL
                     EDKKGLTLVFKNPILYKDDIKWLLNNEPTNILDEEGDQNSWIYILTKKNPEGVKEEKN
                     YDIDIMMEFEDKKLKETTLPERFLENLSKEMLAKIFKSMGKSKVDKSEKKAGTTMEGK
                     KEGLPTMYDVLHTLGEPYEKKEDEEKTELKYFFTVKQPENGAELKPFDLIFDFDVSNK
                     DKVLRKSVVNVNGLKMVMNFVAE"
     gene            3894..4295
                     /locus_tag="AC159U_12885"
     CDS             3894..4295
                     /locus_tag="AC159U_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR3337990.1"
                     /translation="MFGKLLLLFITIPMIELFILVKIGTVIGFWPTVGIVVITGILGA
                     SLARLQGFLVFQQIQSELQSGQIPADKMVDGLLIFIGGVVLLTPGLLTDLAGFAMLIP
                     FTRNITKKYLKSKFEGHIKTKDETKYTVIDV"
     gene            4298..4660
                     /locus_tag="AC159U_12890"
     CDS             4298..4660
                     /locus_tag="AC159U_12890"
                     /inference="COORDINATES: protein motif:HMM:NF040085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HIR3337991.1"
                     /translation="MTHQEKVDHFVEKMKSNGVEKSETAPLLFRLIWKLGFQIPPPQF
                     INIQIIGLVWGLISGICLVLLSLVPAFGFAKLSLFKGILFGAFIGYFMALRHKDRYKK
                     YGLPGWDDYPKESNTSYT"
     gene            complement(4666..5883)
                     /gene="cofH"
                     /locus_tag="AC159U_12895"
     CDS             complement(4666..5883)
                     /gene="cofH"
                     /locus_tag="AC159U_12895"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HIR3337992.1"
                     /translation="MEEIKHVVEKIKDNREAGIDSLLSRVNGTTSKIIEKAYGGNDIT
                     VDEGEQLFLLEDPEQLAALCLAADDIRKEDVGDVITYVINRNINFTNICYTWCGFCNF
                     MAPEGDERAYFLTMDEIKDKVVEAWEHGATEVCMQGGMHPDIDGNYYVDLIKNVKKAV
                     PEMHTHAFSPFEIYYGAKTLDISEEDFIEMLKDVGHNTFPGTAAEVLDEEVRVKIAPR
                     KIPTEVWIRVVKKAHKAGMRTTSTIMYGHMDEPKHWARHLNVLREIQRETGGFTEFVP
                     LRFIPWNTRIFLNSKGKVRPGPTDLDQLKMYAISRLMLRGYINNIQVSWVKQGPEFAQ
                     FSLTAGANDFGGTLMEEQISRAAGANFGQYFPPEEFRRLTWEIDRIPAERTTTYGIRK
                     VFDKKDDSEAVAS"
     gene            complement(5886..7154)
                     /gene="cofG"
                     /locus_tag="AC159U_12900"
     CDS             complement(5886..7154)
                     /gene="cofG"
                     /locus_tag="AC159U_12900"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HIR3337993.1"
                     /translation="MSTTISELENLYSQQTLSAFFDISGLHNDFSKGASSRLQSICSK
                     AEDGQAISREDALYLIRLDYKDILRLLLSATEIRNKGKGNVLSYSKNVFIPVTRLCRD
                     RCGYCTFKIEPGEGELLIPPDEVLETARKGAQLGCTELLFVTGDKPELKYEQYRQALK
                     ELGFSTTAEYLIDLSLKGLKENIFPHTNLGLSTTEELTEFRKTNPSMGLMLENISDRL
                     LYNGEAHHGCPDKVPRLRMKTMELAGELNIPWTSGILVGIGETEEESIDSLFALKSLN
                     DEYGHIQEIIVQNFSPKVGIRMEDKPSPEFMEMIKLVAISRLIFGEKMNIQVPPNLNL
                     ETYPYFTLAGINDLGGVSPLTIDYVNPEAPWPQLELMEEMCEENGFQLRERLPVYPEF
                     INEKYIDNELLPMVNNFVDDTGFVPVQEAN"
     gene            complement(7309..8229)
                     /gene="cofD"
                     /locus_tag="AC159U_12905"
     CDS             complement(7309..8229)
                     /gene="cofD"
                     /locus_tag="AC159U_12905"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR3337994.1"
                     /translation="MITALGGGVGAAKFLSGLIRIGLNQDINIIVNTADDITIGGLRI
                     CPDIDTIVYTLSGIVDKNKKWGLKGDTFNCLEALSRFGIYRWFNLGDKDLATHLYRTH
                     LRSLGITYTEITSRIAKSFGLTDVNVLPMSDSDVETWISSDKGELHFQEYLVREKMLP
                     EVKSIYIKGIENAAATEQVIDSIESASTIILCPSNPIISIGPILRVGGVRDAIKKSKA
                     KKIAISPLVGGLPLKGPADRLMKAEGLEVSSTGIAKLYEDFLDEFVIDSTDKQEALRI
                     EKLGIKATIADTVMKNDQDSEYLAQKILGV"
     gene            8467..10068
                     /locus_tag="AC159U_12910"
     CDS             8467..10068
                     /locus_tag="AC159U_12910"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3337995.1"
                     /translation="MSLELIRSGNKWFTKAVLIVIAVVFIFGFGYSFVNFGAMGGGPS
                     GIAAEVNGEEISLFEYYRLRDSLLSSFGYTADIPDAALPFIRTRALNQLIDLKLISQK
                     ARDLGFKITDEELKQAITSNPAFQIDGDFVGFAAYKNFVEQNLKENVGEFERRYKEEL
                     LAQKFIDFINETAKLSDEELQNLYRIQNERIKLAYLKFSPDQYSDKVQVNKEEISKHY
                     SENKNKYMHPEMREIEYLVIKPSDFEKNIEISDDEVKTYYDSYKDKEFLDEEGNLKDF
                     DAVKDDIIAARMLEKKKIIQQEFIANLDEASKTKKLSEIGKEFAIEKTSKSTISETEQ
                     SQDSVPGAVRAKAFELKKGEKFFINALEDIWIVNLIDIKPESQKSLEEAHSEVVADIK
                     FLKGKNLAKKESKKTHKDLVGGKVKLSSLKDTYKSNYNETDLFTRLDNISEISSKDVL
                     LNAFLLDDKKPVYKEVYESDNQYILITLVEKKQIDLDEFEEKKEEIKNTMIQRKRNEI
                     YQDWILSMRKQANIKPNPNLFPEIG"
     gene            10083..>11763
                     /locus_tag="AC159U_12915"
     CDS             10083..>11763
                     /locus_tag="AC159U_12915"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3337996.1"
                     /translation="MGSSRALIVGSDKDYCYVIKEFLDSRGMICSLILDYNQGIDKLF
                     YENPDITVIELISSHKVSSNLIGKVRNSIELQIIDFTHDSNVEVDKESKPVFFLEETS
                     QLNTLLAFLNTYFLTNSGSEQTSAKSQEPVSNDIELFPYPILLSYLYKNSLSGILTIN
                     SNVNLKLYFEDGVAVYAEGGDVETALGRMLLESGRISDVKDYEKALNIATEKKQKLGE
                     VLVSMGVLSPHELNELLENQIQEKVIRGFLLKSGAFNFESGKSKISNIVKYDIDFPKV
                     LYQGTKRYGNQQFIKNIFFNSGEANNIKVIKDLSQKTGGLGLGPKELRIIQNLEQNTN
                     IENLLNKTTTTNDETLKILFLIYLLGYVVVENDDYRNIGRLFNQASDDPYAGSNEEEF
                     EDIIDLDEEIAESAKAESTKTEVKKELSEKDKEIINTVSKMELEMEIDSPTSTSTSNV
                     IPFKEQAKNPVEEKINIKGPEPPSKEKDNSGLVKSISDLYDRLDEEDHYMILGVDKDS
                     TKQQIRDSYYALVKKYHPDILAKLDDSTRDKADKIFSVITTAYQTLSDDEKR"
BASE COUNT         3927 a         2084 c         2002 g         3750 t
ORIGIN      
        1 atgcaatgaa ttgctgtaaa cacaatatcg ataaacaata tctaataact aaatcagttt
       61 tataaactaa aacaaacagc ttttccatcc cagaataaaa aataaaggcg taagggaggg
      121 ttaattattt attgtaatta ctcattaaaa atcgaatttc ccttttctga aaaacgaggg
      181 ctggaggagc cattttagga ttccttgacg ggcatcaaca aattcccagt tattaatatc
      241 aaattctatt ttggccatgc atgctgtggg gaaattgatc tgtcccccgc caatgaatgt
      301 actggcaagc attgaccact tgggctcgtg gccaacaagc ataatgtcat catacttatt
      361 agatactgat tttattatct caataacagt gcttgtatcc tcatgatata tcctgctatc
      421 ttcctcgaca tcacacttcc actccccaga ctctacagac aatatcagtg tttcttttgc
      481 cctcactgct gtggaagtaa taacaagctc gggcacctga tttgcaagag ccaagacctt
      541 acccataatc tcagcgctat caattcccct ttcattcaga ggcctctcat gatcggaatt
      601 agattccgcc ccccagtcag attttgcgtg tctgtataaa attagtgatt tgggcatgat
      661 taaatttaat tgtacaccta ttgagcatta ttgaaaaata taaatgacac ctttatacta
      721 tttgtaatat aacctacatg taggaggcac acaatgagtg taatgagtat tagaatcgat
      781 gataaaaaac gaaaggctct aaaaatgata gcagcgattg aaggaaagaa tatgagcacg
      841 attgttggtg atcttataga tcagtacata ctgaataata aaaagaaaat tagaaatacg
      901 ctagagcagg aacaactgat tcatttgatg gagctttccg aaacttcatt ctcagaatgg
      961 gataataaag aggatgagat ctacgatgaa ttataaaaag tgggatattg tcttggtccc
     1021 gtttcccttc acagacttaa aaaccacaaa aaagagacct gcacttgtaa tttctcctga
     1081 caactataac aatggttatg atctagtgat cctttttatc acaagccaaa tgaacagtcc
     1141 gaagcgactc ggagattact atatctctga atggaaaaag tggggcctgc ccaaaccatc
     1201 aatgataaga atgaaatttg caaccattca caaatcaata gtaattaaaa cactggggaa
     1261 attaaataat aaagacacaa gcggattaag caagaatttg attgaatttt tgacttaaac
     1321 tagcaatcct atgatgaaac ccaaaaagat attactgata agtttaatcg tccttctgat
     1381 tttatggatt ggtggtaatt tgggtttgta tctattcgct aaacatttcc tgatcggaga
     1441 agacaggtta gagcttttta acaatgtccc tgaaattatt gaaataaaat atacagaaac
     1501 agacgtagaa aaaatctact ttggtgatat agagtttata ttagcaccaa gaggttatga
     1561 tttggtagat atatatcctg gtgtttatat tcaggaacga gaagtagaaa aatattcagt
     1621 ccattcaacc ctaatgttta aatatgcaaa tgaaagaaaa aatattacaa tttattttaa
     1681 caatactgac caaaaactgg tagatgatta tatagagttt tcaaactaca cacttaacga
     1741 ttttagttta tggaatacac tatataatta taattcattc tataaacttg cagcaaaatg
     1801 gatattatta cccagtaaca acgatttgga aaaatatcca ctttatatag tgaattctca
     1861 atatttcaag gggttttgga aagttagttc aggaaaaaaa tcaagtttat tgagtttcga
     1921 ttttagtact aaggagaatt cctatagcat tatgctacct ggtagtgata gagatctaat
     1981 aacggataat atgaaaaaca taatttcttc tataaaacct ttcgaaaaca aagaagaagt
     2041 tactagtgag ttaaagtcta aatataaaaa cgaaacagct atctttgaaa aagagcttgc
     2101 tttgatttca ttaataacaa ttgagggccc aaccgtagaa tatctttcag atcttaaaga
     2161 actgttaatt gaaaaaagtg caagagaaga gaatattgga gatattcagg agcaaattga
     2221 tatattaaaa agtaaattgg acaaagttac taattaacat agaatttttc agttcttgtg
     2281 ccttaataat attagtgatt tacgcatttc taatttatca atataattaa ttcgctgcta
     2341 acttgactga caaataccct gattctggaa aatcagtatc tccatacgat tccgggtatt
     2401 cacctgactt atcaattaat cttagatcta atatgatacc gaatatttga ccaggtctta
     2461 attgaacatc tttatctttg cttgccagcg gatgagaaaa ttcatagtat gaatagttat
     2521 tatctgtact atatgtacct agtccatcgc gggtaccccc tactttagta tcttcgaatc
     2581 cgcatttgcc ttcataatat ttgccatctg gcattgaaca ggggggttgg cttgtcctca
     2641 cactatcaat taagtaattg ggtttatgaa cagggttcat acctataaca tcttgaccat
     2701 cataaataaa tttaccgttt ttatcattaa atctaataaa gaaactttgt cctacaatat
     2761 ctttaggagt tttgaatttg aacagaaaat agatgttgtt tgaatcattt aaaactttta
     2821 acactgcata tgatttcttg ctaaatggta aattgatatt aagtttaaat gcttctgcat
     2881 aactccatgc taaatcatga ggtttgccat ctacttcatt tagtgaacca aatttagatt
     2941 caatatctgc agcatacaag acacctatac aaatataact aattaatatc aataatatta
     3001 ttttcttaaa catagaaata attatctgta tcaattaact actcagcaac aaaattcata
     3061 accattttaa gtccgttaac attcacaaca gatttacgga gaactttatc tttattactt
     3121 acatcaaaat caaaaatcag atcgaaaggt ttaagctcag caccgttttc aggctgcttt
     3181 acagtgaaga aatatttaag ctcggttttt tcctcatctt cctttttttc ataaggctcg
     3241 ccaagtgtat ggaggacatc atacatagta ggcagacctt cttttttgcc ctccatagta
     3301 gtgccggctt ttttttccga cttatcaact tttgatttac ccatggattt aaatatcttc
     3361 gcaagcatct cttttgacag gttttctaga aatctttctg gtagtgtagt ttctttaagc
     3421 ttcttgtcct caaactccat catgatatca atgtcataat ttttctcttc ttttactcct
     3481 tctggatttt tctttgtaag tatataaatc cagctattct gatctccttc ttcatctaaa
     3541 atatttgtag gttcattgtt gagcagccac ttaatgtcat ctttataaag aataggattt
     3601 ttgaaaacca gagttaggcc ttttttgtct tcaagctcga agttttcttc aaaatttgca
     3661 aactggcgct ttagttttag gagtctgtag tagacacagc cggaaagcaa tgtggttata
     3721 agtaaaaaaa taatgcagat aaaatatttt ctattaatca tcataaaact cggttgtagg
     3781 aatttcgcaa tagaataacc tagtttggta attatttcat atacgctaca atcatattag
     3841 atttcagtga ttaccttgat taaatcagaa aaggtattac atattagatt ataatgtttg
     3901 gaaaacttct actattattt attacaattc cgatgataga gcttttcatc ctggtaaaga
     3961 ttggaactgt tataggattt tggcccactg ttggtattgt agttatcaca ggaattctgg
     4021 gagcttctct tgctaggctt caagggtttt tggtattcca gcagattcaa agtgaattac
     4081 agagcggaca gatacctgct gataaaatgg tagacggtct gctaatattt ataggcgggg
     4141 ttgttcttct tacccccggg cttctaactg atcttgctgg atttgcaatg cttatccctt
     4201 ttaccaggaa tattacaaaa aaatacctca aatcaaaatt cgaaggtcat atcaaaacaa
     4261 aagacgaaac caaatatacg gttattgatg tttagaaatg actcatcagg aaaaagtaga
     4321 tcattttgtc gagaaaatga aatccaatgg tgttgaaaaa tcagaaacag cgccactttt
     4381 atttagattg atatggaaac taggatttca aattcccccg ccccaattta ttaatattca
     4441 aattatagga ttagtctggg gattaatatc tggaatttgc cttgtattat tatcactagt
     4501 tccagcgttt ggatttgcta agttatcttt atttaaaggc atcctttttg gagctttcat
     4561 tggatatttt atggctctta ggcataaaga tcggtacaaa aaatatggcc ttcctgggtg
     4621 ggatgactat cctaaagaaa gtaatacttc atatacttga tgatactagc ttgctactgc
     4681 ctcagaatca tcttttttat cgaatacctt tctaattccg taagtagtcg tccgttctgc
     4741 tggtatcctg tcaatctccc aggtaagtct tctaaattcc tcaggtggga agtactgacc
     4801 aaaattggcg cctgcagctc ttgaaatctg ctcttccata agagtgccgc caaaatcatt
     4861 tgcaccggca gtgagtgaaa actgagcaaa ttccggtcct tgtttaaccc aggagacctg
     4921 aatgttgttt atataacctc ttagcataag cctagaaata gcatacatct ttagctgatc
     4981 cagatctgtt gggccagggc gtaccttacc tttagagtta agaaaaattc ttgtgttcca
     5041 tggtataaat cttaaaggca caaactcggt gaacccgcca gtctctcttt gtatctcgcg
     5101 cagtacattc aaatgccttg cccagtgttt aggctcgtcc atatggccat acataattgt
     5161 agaagtagtc ctcattccgg ctttgtgcgc ctttttaaca acccttatcc agacttcggt
     5221 tgggatcttg cgtggcgcaa ttttaaccct tacttcttca tccaatactt ctgctgcggt
     5281 tccgggaaaa gtgttatgtc caacatcttt taacatctca ataaaatctt cttcactgat
     5341 atctaaagtt tttgcgccgt aataaatctc aaatggtgaa aatgcatgag tatgcatttc
     5401 gggtactgct ttttttacat ttttaatgag gtcaacataa taattaccgt ctatgtcagg
     5461 atgcattcca ccctgcatac agacttccgt tgcgccgtgc tcccatgctt ctaccacttt
     5521 gtcctttatc tcatccattg tgagaaagta agccctttca tcaccctcag gagccatgaa
     5581 attgcaaaac ccgcaccatg tgtagcaaat attagtaaag tttatgtttc tgttaataac
     5641 ataagtgata acatcgccga catcctcttt tctgatatca tcagcagcaa ggcaaagtgc
     5701 tgctaactgc tcggggtctt caagtaaaaa gagctgctcg ccctcatcaa ctgtaatatc
     5761 atttccgccg taagcttttt caattatttt agaagtcgtt ccattaaccc tggaaagaag
     5821 actgtctata ccggcttctc tgttatcttt tattttctct actacatgct taatttcttc
     5881 cattactagt ttgcctcctg caccggaaca aaacctgtat catctacaaa attgttcacc
     5941 ataggtaata gttcattatc aatatatttt tcatttataa attccgggta gaccggaagt
     6001 ctttccctta actgaaatcc attttcttcg cacatttcct ccataagctc tagctgcggc
     6061 cagggagcct cggggtttac ataatctata gtaagagggg atactcctcc aagatcgtta
     6121 atccctgcaa gagtaaaata aggataggtc tccaaattaa gatttggagg tacctgaata
     6181 ttcatctttt caccaaatat tagtcttgaa atcgccacca gttttatcat ctccataaac
     6241 tctggtgatg gtttgtcttc cattcttatt ccaaccttgg gactaaaatt ctgaacaatt
     6301 atttcctgaa tatgcccgta ctcatcattt agactcttaa gagcaaaaag ggaatctatg
     6361 ctttcttctt cagtttctcc aataccaacc aaaatacctg aagtccaggg gatatttaac
     6421 tcacctgcaa gctccatggt tttcattcta agccttggta ctttatcggg acagccatga
     6481 tgagcttctc cattgtaaag cagcctgtca cttatgtttt caagcataag acccatactg
     6541 gggttggttt ttctaaattc agtcaactcc tcagttgtag acagtccaag atttgtatgt
     6601 ggaaatatat tctctttaag tccttttaag cttaaatcta tcagatattc ggcagtagta
     6661 ctaaatccaa gctcttttag ggcttgcctg tattgttcat atttaagctc aggtttgtca
     6721 cctgttacaa ataaaagttc agtacagcca agctgggctc cttttcttgc tgtttccagc
     6781 acttcatcgg gtgggataag tagctcgcct tcaccgggtt ctattttaaa agtacagtaa
     6841 ccacatctgt ctctgcaaag acgggttaca ggtataaata catttttgga ataggatagt
     6901 acgtttcctt ttcctttatt tcttatttca gtagcactaa gcagcaatct taatatgtct
     6961 ttgtagtcca gtctgataag ataaagagca tcttctctac ttattgcttg accatcctct
     7021 gcttttgaac aaatagactg tagtcttgag gatgcaccct tgctaaaatc attatgcaaa
     7081 cccgaaatat cgaaaaatgc cgatagagtc tgctgagaat acaaattttc cagttcgctt
     7141 atagttgtac tcataacaat gttttattaa ttattgttga acgttcatta aaataattat
     7201 aacagtaaat ataaaatttg ttttgattat tgttttttta tcttacccca aactatattc
     7261 acatatttaa aatgttattg tcaagtagga aatataatag aatactgtct aaacaccaag
     7321 gattttttgt gcaagatatt ctgaatcctg atcatttttc attacagtat ccgctatagt
     7381 agcctttata cccagttttt ctattctcag ggcctcttgt ttatcggtac tatcaataac
     7441 aaattcatct aaaaaatcct cgtacagctt agctatccca gtggacgaaa cctcaaggcc
     7501 ctctgctttc attaacctgt cggcaggacc ctttaggggt aatcctccta caagaggact
     7561 tatcgcaatt tttttagctt tggacttttt aattgcatct ctaacaccac ctactcttaa
     7621 tattggtcca atgcttatta tagggttact ggggcatagg attatagtgc tggcagactc
     7681 tatagaatca attacctgct cagttgctgc cgcattttct attcctttta tataaattga
     7741 tttaacctca ggaagcattt tttctctaac caaatactcc tgaaaatgta attcgccttt
     7801 atcgctgctt atccatgttt caacatcgct gtcactcata ggaagtacat taacatctgt
     7861 taagccaaaa gattttgcaa ttctagatgt aatttcagta taagtaattc caagcgatct
     7921 tagatgagtc ctatataaat gcgttgcaag gtctttatca ccaagattaa accagcggta
     7981 gattccaaac ctgctaaggg cctcaaggca gttaaaggta tctcccttaa gaccccattt
     8041 tttattctta tcaacaattc cggaaagcgt ataaacaatt gtatcaatat caggacatat
     8101 tctcagtcca ccaatggtta tgtcatctgc agtattaaca attatgttta tgtcttgatt
     8161 taatcctatc ctaataagac cagatagaaa ttttgcggca cctactccgc cacccagagc
     8221 tgttatcata aatttttcat ccctctgtat aatatgtacc catattatta tcttatactt
     8281 ttattaaaac caatatatta ttctaattct taacttaatt tatagttctt tatggcattt
     8341 tctttagttt tcatttttaa tctggcataa aactttagat tgtttattct taaatttgtt
     8401 atatattaaa gtgtttacgt aagctgttat tagacagtaa attattgtat atttaaaggg
     8461 agtattatga gtttagagct aataagatca ggtaataaat ggtttacaaa agctgtatta
     8521 atagtaatag ctgtagtatt tatatttggc tttggatatt cattcgtaaa ttttggagcc
     8581 atgggaggcg gacccagtgg tattgcggct gaggttaatg gagaagaaat ttcacttttt
     8641 gaatattaca gactaagaga cagcctgtta tcaagttttg gttatactgc cgacattccc
     8701 gatgcagcat taccatttat acgtacaaga gccttaaatc agttaataga tctaaagctt
     8761 atttctcaaa aagcgcgtga ccttggattt aaaatcacag atgaagaact taaacaagca
     8821 ataacatcaa accccgcttt tcagattgat ggagattttg tgggatttgc agcttacaaa
     8881 aattttgttg agcaaaatct aaaagaaaat gtaggagagt ttgaaagaag atataaagaa
     8941 gagttattgg ctcaaaaatt tattgatttc atcaatgaaa ctgcaaagtt atcagatgaa
     9001 gaactgcaaa atctttatag aattcaaaat gagagaataa aacttgcata tctaaagttt
     9061 tcacctgacc agtactccga caaagtgcag gttaacaaag aagagatttc aaaacattac
     9121 agtgaaaaca agaataaata catgcacccc gaaatgagag aaattgaata tttagtaatc
     9181 aaaccctctg actttgaaaa aaatatagaa atatccgatg atgaagttaa aacttattat
     9241 gattcttata aggataagga attcttggat gaagaaggaa atctaaaaga ctttgatgca
     9301 gttaaagatg acattatagc agctcgtatg cttgaaaaga aaaaaataat acagcaggaa
     9361 tttatcgcaa atctcgatga agcatcaaaa accaaaaagt taagtgaaat tggtaaagaa
     9421 tttgctatag aaaaaacctc taagtcaaca atttctgaga ctgaacaatc acaagattca
     9481 gttcccggtg cggtaagagc taaagccttt gagcttaaaa agggagaaaa gtttttcatt
     9541 aatgctttag aagatatatg gatagtaaac ttaatagata ttaagcccga gtctcaaaag
     9601 agccttgaag aagcacatag cgaagtagta gcagacatta aatttttaaa aggtaaaaat
     9661 cttgccaaaa aggaatcaaa aaagactcac aaagaccttg ttggtggcaa ggtaaaatta
     9721 tctagtttaa aagataccta taaaagcaat tacaacgaaa ctgatctgtt tactaggctt
     9781 gataatataa gtgaaataag ctctaaggat gtgttgctta atgcttttct tctagatgat
     9841 aaaaagccgg tgtataaaga ggtatatgag agtgataacc aatatatatt aattaccctt
     9901 gtggaaaaaa agcagataga tctggatgaa ttcgaagaga aaaaagaaga aattaaaaat
     9961 actatgatac aaagaaaaag aaacgaaatt tatcaggact ggattttatc tatgcggaaa
    10021 caagctaaca taaaacctaa tccaaacctg tttccagaga ttggataaaa ggagcaaaat
    10081 ttatgggctc ttcaagggct ttaatagttg gctctgataa agattattgc tacgtaataa
    10141 aggagttctt agattcccgt gggatgattt gttctcttat tcttgattac aatcagggaa
    10201 ttgacaagct tttctacgaa aatcccgata ttacagtaat tgagcttata tcatctcata
    10261 aagtaagttc aaacctcata ggaaaagtta gaaactccat agaattacaa atcatcgact
    10321 ttactcatga cagtaacgtc gaggttgata aggaatcaaa accggtattc ttcttagaag
    10381 aaactagtca gctcaatacg cttttagctt ttctcaatac ctattttctt acaaactcgg
    10441 gcagcgagca aacatctgcc aaatctcaag aacctgttag taacgacatt gaattatttc
    10501 catatccaat acttctttcc tatttataca aaaactcact ctcgggaata ctgacgataa
    10561 attcaaatgt aaacctcaag ctttactttg aagacggtgt agcagtatat gccgaaggtg
    10621 gagatgtaga aactgctctt ggaagaatgc ttcttgaaag tggaaggatt agtgatgtaa
    10681 aagactatga aaaagcactg aatatagcaa ccgagaaaaa acaaaagctt ggagaagttc
    10741 ttgtttcaat gggcgttctt tctccacatg aactaaatga actattagaa aatcagatac
    10801 aggaaaaagt aataagaggt tttctattaa aatcaggtgc atttaacttt gagagtggta
    10861 aaagtaaaat cagtaacatt gttaaatatg atattgattt tcccaaggtt ttgtatcagg
    10921 gtaccaagag gtatggaaac cagcagttta ttaaaaatat atttttcaat agtggagaag
    10981 ccaataatat taaggtcatc aaagatctca gccaaaaaac aggcggtctg ggtttgggcc
    11041 ctaaagagct tagaataatt cagaaccttg aacaaaacac taatatcgaa aatttactga
    11101 acaaaacaac tactactaat gatgaaacat taaaaatcct gtttttgatc tacctgctgg
    11161 gatatgtagt tgttgaaaat gatgattaca gaaatatagg cagattgttt aatcaggcat
    11221 cggacgaccc gtatgcaggt tcaaacgaag aagaatttga ggatataatt gatctggatg
    11281 aggaaattgc agagagtgcc aaggcagaat ctacaaaaac tgaggtaaag aaagagctat
    11341 ccgaaaaaga taaagaaata ataaatacag ttagtaaaat ggaactcgaa atggagatag
    11401 attctccaac atcaacgagt acatctaatg tcattccatt taaagaacaa gccaaaaacc
    11461 cggtggagga gaaaataaat attaaagggc ccgaaccacc gtccaaggaa aaagataact
    11521 ctggtctggt aaaaagcata agtgacttat atgacaggct cgatgaagaa gatcattata
    11581 tgatattagg ggtggataaa gattctacaa aacagcaaat aagggatagt tattatgcat
    11641 tggtgaagaa atatcatcct gatattcttg caaaactcga tgacagcacc agagacaaag
    11701 ccgataaaat attttctgtg ataacaacgg cctatcaaac actaagtgat gatgagaaaa
    11761 gag
//
LOCUS       DBNGSV010000364         7736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_620217, whole genome shotgun sequence.
ACCESSION   DBNGSV010000364 DBNGSV010000000
VERSION     DBNGSV010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_620217"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC159U_12920"
     CDS             complement(<1..192)
                     /locus_tag="AC159U_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3337997.1"
                     /translation="MEIKYQEYEEQQPKQEQSESYADSDKDNLKQLGIAGSAVAEMDE
                     DEFEFEQQPTTKELQKIEEE"
     gene            complement(360..968)
                     /locus_tag="AC159U_12925"
     CDS             complement(360..968)
                     /locus_tag="AC159U_12925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3337998.1"
                     /translation="MPHTLPELSYDYNALEPHIDAKTMEIHHTKHHQTYINNLNAALE
                     SHPDLQDKPVEDLIRDLGSVPEAIRVAVRNNGGGHLNHSMFWTVMGPGAGGQPSGDLA
                     SAIDSAFGSFSDFQDAFAKAGATRFGSGWAWLCVDGGKLVVTSTPNQDNPVTDGLTPI
                     LGLDVWEHAYYLNYQNRRPDYIKAWWNVVNWDQVSANLSAAK"
     gene            1204..1980
                     /locus_tag="AC159U_12930"
     CDS             1204..1980
                     /locus_tag="AC159U_12930"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3337999.1"
                     /translation="MKCTGCGFNTFDYLESCNKCGNVFESNFQFKKLYAKYSEPRKLK
                     RNNKSDGLDPAGINKTNINLNRHKIIIPDDSKYTPSVKVSDNLNLHAQPSKSGLQGNE
                     KEDIFEPDFKYDSSNIYVQASTESRIAASFIDFIILNILTLVCICCALLILGYKIDKS
                     LLLIKEHVILIYLVLLFLSTSYFVLMQGFGGRTIGKLLLSLKVIKKDGIEIGFIDSFI
                     RFIGYFISAVPVFLGFIWSYIDSNSQAWHDKIAGTVVIEK"
     gene            2162..3430
                     /locus_tag="AC159U_12935"
     CDS             2162..3430
                     /locus_tag="AC159U_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3338000.1"
                     /translation="MSVEHAGNFPADKFLLVLAIILIGSKLIGELTERIKQPPVLGEL
                     IAGVILGGSVLALVPSVAGDAGYQTFHLLAELGVCILLFEIGLETDLDELLKVGDTSA
                     IVAIVGIVAPFVLGFISIMGFQQYGLLTFDSPATGTLVAITVGATLTATSVGITARVL
                     SDMNKIHSPESKIVLGAAVIDDILGLVILAVVTGLIEASKQGLDTGISAVSVLTISAK
                     AFGFLIVSIIAGILVSGKIFNFFHKLKVRGILLLSAVGFALLYSYLAHKAGLAAIVGA
                     FAAGLVVAKTAQVKVIEERLKPVSDLVTPIFFVMVGAAVDVTVFNPFNSGNIPILIIA
                     MVLFAVAVAGKFVSGFVVFAKDIKKKVIGIGMIPRGEVGLIFAQVGLAFGVFDIKLFS
                     AVTAMVMLTTFVAPPLLKKSFGSDESENKS"
     gene            3459..3968
                     /locus_tag="AC159U_12940"
     CDS             3459..3968
                     /locus_tag="AC159U_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HIR3338001.1"
                     /translation="MNRMIATLYILLIILSTTANVFAASDDILSVDPKHLGILIVLFV
                     ASIVLLNKILFQPLLDLDEKREQLTSGTSSEAKDLKKKAQDTINEYTEKINEARLETQ
                     EQRNLIRKEAQSSAAEMMERARGEANSLLDEAKTKIDNEAKEIREKIKPEIELIAKDV
                     ASKLINKEI"
     gene            3971..4522
                     /locus_tag="AC159U_12945"
     CDS             3971..4522
                     /locus_tag="AC159U_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B family protein"
                     /protein_id="HIR3338002.1"
                     /translation="MVEIESLISLAVTMAGGGADAFNWQYVGKHAANLAILVGVLVYF
                     LKTPVKNFLMERRGQIASKIESSEKEITEAKALFDQYMAKMNNLEQEIKDLKDTIKNE
                     AELERQEIIKQAEVSAQKIREDAKETIKSETAKAKHQIQGEVVQLAMQLAENLIKSNL
                     NENDSKRIIEDFVSEVNETKWQQ"
     gene            4510..5052
                     /gene="atpH"
                     /locus_tag="AC159U_12950"
     CDS             4510..5052
                     /gene="atpH"
                     /locus_tag="AC159U_12950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR3338003.1"
                     /translation="MATVATLRDLISALIETSVQSFKLSTTTSDLEKFFSVIEQNEDL
                     KDIFASTVYELDEKLAILNDVNAQIGMDAYTVNFIKSVIEMDKVKGLVDSRELVLRRL
                     RKAAGIELAEFIFAKVPEETEVHGVQQAIEAQIGNKIEVAVTVDPEILGGVIVKVSNK
                     LFDNSIRTKLNKVKNMLTAA"
     gene            5083..6600
                     /gene="atpA"
                     /locus_tag="AC159U_12955"
     CDS             5083..6600
                     /gene="atpA"
                     /locus_tag="AC159U_12955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3338004.1"
                     /translation="MEDIKIEGIGEILKNRIKGYDQKIETAEVGTVISVGDGISRAYG
                     LDNTMAGELVEFQNGIMGLVLNLEEDNVGIAIFGEDTQIQEGDTVKRTGKIAEIPVGD
                     NVVGRVVDALGQPIDGKGEIQTTDTRQIEVKAPGVVYRQSVNEPLQTGIKAIDSMIPI
                     GRGQRELILGDRQTGKTAVAVDTIINQKGQDVYCIYVAVGQKQSTVAQVVDKLREHGA
                     MEYTTVVAATASDPAPYQFISPYTGCAIGEFFRDSGRHALVIYDDLTKHAWSYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLRDADGGGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQIYLESDLFYAGIRPAINVGLSVSRVGGSAQIKAMKNVAGTLRLELAQYRE
                     VEAFAQFASDLDKVTQAQLARGSRLVEALKQVQYAPLAVEKQVLMIFAVTNGYVDDYP
                     VETMGQYEKELYIHLEANHPEILTEIREKKVIDDDLKTKVISALDSLKDKLGELK"
     gene            6624..7520
                     /gene="atpG"
                     /locus_tag="AC159U_12960"
     CDS             6624..7520
                     /gene="atpG"
                     /locus_tag="AC159U_12960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR3338005.1"
                     /translation="MASLRQISNKISSVKGTRRIMSAMKLIAAVKLQKAQSLLTAYRP
                     YSDSYLDIAKGLANNSDEGLHPLLKNPEEKKNLHLVFMTSDRGLCGSFNSSLIRKVET
                     YLLTETQDYEKVKITFVGRKGRDHFKKSEIEKAGEHSGITERNYEEYTKGIADEIAGN
                     FMSGKSDEVILIYNYFQSALTQEMQMEQLLPIPRTAVEEGEEETEKQGEYIFEPNKNE
                     VLKHVLSNYVEVRVQRALLESVTSEHGSRMTAMENATRNADSVIRSLTLLFNKTRQAS
                     ITTELMDIVNGTEALRKGGGMD"
     gene            7520..>7736
                     /locus_tag="AC159U_12965"
     CDS             7520..>7736
                     /locus_tag="AC159U_12965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423393.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3338006.1"
                     /translation="MEATTQNTGKIIQVMGPVVDVEFKDSSLPAIYTALRVTNKLISE
                     EDWNLVLEVAQQLGSKRVRCISMDSTEG"
BASE COUNT         2521 a         1303 c         1660 g         2252 t
ORIGIN      
        1 ttcctcttct attttctgca gttcttttgt tgttggttgt tgttcgaact caaattcatc
       61 ttcgtccatc tcggctaccg cactgcctgc tatgcccagc tgctttaaat tgtctttgtc
      121 gctgtcagca taggattcag actgttcttg ctttggctgc tgctcttcat attcctgata
      181 ttttatttcc atgattactt cctgattaat aatatataat tataactcaa cttattatgt
      241 tttgagtgtt ttaagggctg caatattaaa caaattactg atcgggtagt atggtaaaaa
      301 tgatactctc aatagtttag gatgatcagt aacttaaagt cttgcaaaat aaatctatac
      361 tattttgctg cacttaagtt tgcagaaacc tgatcccagt ttactacatt ccaccatgcc
      421 ttgatgtagt ctggtcttct gttctgataa tttaaataat atgcatgttc ccaaacatcc
      481 aaacctaaaa tcggagttag tccatctgtc acggggttat cctggtttgg agttgatgta
      541 acaactaatt tgccgccatc aacacaaagc catgcccacc cgcttccaaa cctggtagca
      601 ccagccttgg caaaagcatc ctgaaaatca gagaaactgc caaaagcaga gtctatggct
      661 gatgctaaat caccagatgg ctgcccgcct gcacctgggc ccatgacagt ccagaacata
      721 ctgtggttta aatgacctcc accgttgttt cttacagcaa cccttatagc ttctggaaca
      781 ctgccaagat ctctaattaa atcttcaaca ggtttatcct gtagatcggg gtggctttct
      841 aaagctgcgt tcaggttatt gatatatgtc tgatggtgtt tggtatgatg aatctccatt
      901 gttttagcgt caatatgagg ctcaagcgca ttataatcgt aagataattc cggtaatgtg
      961 tgtggcattt aaatatgaat cctcctgtaa tagaattaaa tattatttca atttagtcat
     1021 ggctaagata gcctatattt ccactataac gtatacgttt tgagaatgca atatgttttg
     1081 taactgcttt aaaatattag ttttttacca ataaaaacgc cttaagttta ttactgatat
     1141 tgatttatgg cacttacgtt gtatcattaa tgtgttgctt taagtgtggg gtgcaattga
     1201 attatgaaat gtacaggttg tgggttcaat acatttgatt atctggaaag ttgtaataaa
     1261 tgtggaaatg tttttgagtc aaatttccag tttaaaaagc tttatgcgaa gtactctgag
     1321 ccaagaaaat taaagagaaa taacaaatct gatggtttgg acccggctgg gataaataag
     1381 acaaacatca atttgaaccg tcataaaata attattccgg atgattctaa atacactcca
     1441 tctgtaaaag tatccgataa cttaaattta catgctcagc catctaaatc cgggctacag
     1501 ggtaatgaga aagaggatat atttgagcct gactttaaat atgatagtag caatatttat
     1561 gtacaggcat ctacagaatc gaggattgcc gcttcattta tagattttat tatattaaat
     1621 attttgacac tagtttgtat ctgctgtgcc ttattgattt taggatacaa gattgataaa
     1681 agtttactgc tgataaaaga gcatgtaatt cttatttatc ttgtattgct gttcttaagt
     1741 acatcttatt ttgtgctaat gcagggattt ggaggcagga ctataggtaa attgttgctg
     1801 agccttaaag taataaaaaa agacggcata gaaataggtt ttatagattc atttattagg
     1861 tttatcggtt acttcatttc ggcagttcct gtgtttttag gatttatatg gtcttacata
     1921 gattcaaact cacaagcctg gcatgacaaa atagcaggta ctgtagtaat agaaaaatag
     1981 acatttttct aattatcatt acccttgtca tcattaaacg ctattttata tcagtttttc
     2041 cctaaaactc tttaaatttt gttcatttca tatcattgac acttaactcc ttacatgtta
     2101 gaaccttctg gtttttttaa aacttctggg tatttcctaa aaaatttaat attaaaaatt
     2161 tatgagtgtt gagcatgcag gcaattttcc tgcagataag tttcttttag ttttagcgat
     2221 aattcttatc ggttccaagc ttattggcga gcttaccgaa agaatcaaac agcctccggt
     2281 tctaggagag ctaatagcag gggtgattct tgggggtagc gtacttgcgc ttgtaccctc
     2341 ggttgccggt gatgccggtt atcagacttt tcatctttta gcagaacttg gtgtttgtat
     2401 actcctgttt gagataggtc ttgaaactga tcttgatgaa ttgttaaaag tgggtgatac
     2461 ttcagcaatt gtagccattg tgggaattgt agcgccattt gtgcttgggt ttattagtat
     2521 aatgggtttt cagcaatatg gccttctaac ttttgacagt cctgcaacag gtactctggt
     2581 tgcaattaca gtaggtgcca ccctaaccgc taccagtgtt ggtataacag caagagtttt
     2641 atcggatatg aacaagattc atagccctga gtcaaagata gtccttggtg ctgctgtaat
     2701 agacgatatc ttggggctag taatacttgc agttgttacg ggtcttattg aagcgtcgaa
     2761 gcagggatta gataccggga tatcagctgt aagcgtattg acaatatcgg caaaagcttt
     2821 tggttttctt attgtatcca taatagcagg aattcttgta tctggaaaaa tatttaattt
     2881 ctttcataaa ttgaaggtta gaggaatact tctattaagt gctgtaggat ttgctctgct
     2941 ttattcatac ctggcacata aagcggggct tgctgccatt gttggagctt ttgccgcagg
     3001 acttgtagtc gctaaaacag ctcaggtaaa ggtaatagaa gaaagattaa aaccggtttc
     3061 agaccttgta acgccgatat tctttgtgat ggtaggtgct gcagtagacg taaccgtttt
     3121 caatccgttt aattcaggta atatccctat ccttattatt gcaatggtat tgtttgctgt
     3181 cgcagttgct ggtaagtttg taagcggctt tgtagttttt gctaaggata taaagaaaaa
     3241 agttataggt atcggtatga ttccaagagg agaagtagga ttaatatttg ctcaggttgg
     3301 acttgctttt ggagttttcg atataaaact cttttcagcc gtgacagcta tggtaatgtt
     3361 aacaactttt gtagcaccac cgcttcttaa aaagtcattt ggaagcgatg aaagtgagaa
     3421 taaaagttga ttatttgtat ttatagtatg gagtaaaaat gaatagaatg atagctacat
     3481 tatatatatt gctaattata ctcagcacta cagcaaatgt atttgctgcc agtgatgaca
     3541 ttctgagtgt tgatccaaag catctgggaa ttttaatcgt tctttttgtt gcaagcatag
     3601 ttcttctaaa caaaatactt tttcagcccc ttttagacct tgatgaaaaa agggagcagc
     3661 ttacaagcgg aacaagctct gaggctaaag accttaagaa aaaagctcag gacactatta
     3721 atgaatatac tgaaaagata aatgaagcca gattggaaac gcaggaacag agaaacctaa
     3781 taagaaaaga agcccaaagc agtgcagcgg agatgatgga aagagcaaga ggcgaagcaa
     3841 actcactgct tgatgaagca aaaaccaaaa tagataacga agcaaaagaa attagagaaa
     3901 aaataaaacc tgagattgag cttatagcaa aagatgtggc ctcaaagctt attaataagg
     3961 agatatagag ttggttgaga ttgaaagttt aataagtctt gcagtaacaa tggcaggcgg
     4021 tggtgcagat gcatttaact ggcagtacgt tggcaagcat gcggctaacc tcgccatttt
     4081 agtgggtgtt ctcgtttatt ttcttaaaac gcctgtaaaa aactttctta tggaaagaag
     4141 agggcagatt gcaagcaaaa tagaaagctc tgaaaaagag attactgaag caaaggcact
     4201 tttcgatcag tacatggcaa agatgaacaa tcttgagcag gaaattaaag accttaaaga
     4261 tacaataaaa aatgaagccg aattggagag acaggaaata ataaagcagg ccgaggtatc
     4321 agcacaaaaa ataagagaag atgccaagga aaccattaaa tctgaaactg ctaaggcaaa
     4381 acatcaaatt cagggtgaag tggtgcagct tgcaatgcag cttgcagaaa accttatcaa
     4441 aagcaacctt aatgaaaatg acagtaaaag aattattgaa gactttgtaa gtgaggtaaa
     4501 cgaaacaaaa tggcaacagt agcaacccta agagatttaa tatcggcgct tattgagacc
     4561 tcggtccagt cattcaaact ctcaactacc acatctgacc ttgaaaaatt tttctcagtt
     4621 attgagcaaa atgaagactt aaaagatata ttcgcaagca ctgtttatga gcttgatgaa
     4681 aagttagcaa tcctaaacga tgtaaacgca caaattggta tggatgcata tacagtaaat
     4741 tttattaaat cagtaattga aatggataag gttaaggggc ttgttgattc aagagaactt
     4801 gttcttagaa gactcagaaa agccgcagga attgagcttg ctgagtttat atttgcaaaa
     4861 gtgccggaag agacagaagt acacggcgtt caacaggcta ttgaagctca gataggaaat
     4921 aaaattgagg ttgctgtcac ggttgatcct gaaattttgg gtggggtgat cgtgaaagta
     4981 tccaacaaac tatttgataa cagtataaga acaaaactaa ataaagttaa aaatatgctt
     5041 acggctgcat agcagttttg gaggataaaa ggagtcagat aaatggaaga tataaaaatc
     5101 gaagggattg gagaaatact taaaaaccga atcaagggtt acgatcagaa aatagagacc
     5161 gctgaggttg gaactgtaat ctcagtagga gatggtattt cgcgtgcata cggactcgac
     5221 aacacaatgg ccggagagct cgtagagttt caaaacggca ttatgggtct tgtactaaac
     5281 cttgaagaag ataacgtagg tattgcaata tttggtgaag acacacagat tcaggaaggc
     5341 gatacagtaa aaagaactgg aaagatcgcc gaaattccag ttggtgataa tgtagtagga
     5401 agggtagtag atgctcttgg tcagccaatt gatggtaaag gtgaaattca gacaacagat
     5461 accagacaaa ttgaggttaa ggctccaggc gttgtttaca gacagtcagt gaacgagccg
     5521 cttcagacag gtattaaagc aattgactcc atgattccaa taggtagggg acaaagagag
     5581 cttattctcg gtgacagaca aacgggaaag acagcagttg cagttgatac tattattaat
     5641 cagaaagggc aggacgttta ttgcatatat gtagcagtag gacagaagca gtctacagtt
     5701 gcccaggttg ttgacaagct aagagaacac ggtgcaatgg aatacacaac agttgttgct
     5761 gctactgcaa gtgaccccgc accttatcag ttcatctctc cttatacggg atgtgctatt
     5821 ggtgaatttt ttagagattc aggaagacac gcacttgtga tttatgatga tctaacaaaa
     5881 cacgcatggt catacagaca gatttcactt ttactcagaa gacctcctgg acgagaagct
     5941 tatccaggag acgtttttta tctacactca aggcttcttg aaagggctgc taaattaaga
     6001 gacgcagacg gcggcggttc acttacagcg cttccgatta ttgaaactca ggcaggtgac
     6061 gtatctgcct atattcctac aaacgtaatt tctattacag atggtcagat ttatcttgag
     6121 agtgacctgt tttatgccgg tatcagacct gctattaatg ttggtctatc ggtatcaagg
     6181 gtaggtggat cagctcagat caaggctatg aaaaacgtag caggaactct gaggcttgag
     6241 cttgctcagt atagagaagt ggaagctttt gctcagtttg catcagacct tgataaggtt
     6301 acccaggctc agcttgcccg tggtagccgt cttgtagaag cgctaaagca ggttcagtat
     6361 gcgccgctag ccgttgaaaa acaggtactt atgatatttg cagtaacaaa cggatacgtt
     6421 gatgactatc cagtggagac aatgggtcaa tatgaaaaag aactctatat tcacctagaa
     6481 gccaaccatc cagaaattct cacagaaata agagagaaaa aggtaataga tgatgatctt
     6541 aagaccaagg tgataagcgc actcgactcg ctaaaagata aacttggtga gctaaaataa
     6601 taaatattga gaacatataa caaatggcaa gtttaagaca aataagtaat aaaatatcga
     6661 gcgtaaaagg taccagaaga attatgagtg ccatgaagct cattgcagca gtaaaactgc
     6721 aaaaggcaca gtctcttctt acagcttaca gaccctactc ggattcttat ctggatattg
     6781 caaagggtct ggccaataac tctgatgaag gactgcaccc gctgttaaaa aaccctgaag
     6841 aaaagaaaaa tctgcatctt gtgttcatga cttctgaccg tggtctttgt ggtagtttta
     6901 acagcagttt aattagaaaa gtagaaacct atctgcttac agaaacccag gattatgaaa
     6961 aggttaaaat cacttttgtg gggcgaaagg gaagggatca ttttaagaaa agtgagattg
     7021 aaaaagccgg tgagcattcg ggtattacag aaagaaacta tgaagaatat accaaaggca
     7081 tagcagatga gattgccggt aattttatga gtggaaagtc tgatgaagtt atccttatct
     7141 ataattactt tcaatcagca ctcacccagg aaatgcagat ggaacagctt cttccaattc
     7201 caagaacagc agtagaagaa ggtgaagagg aaacagaaaa acagggtgaa tatatatttg
     7261 aaccaaataa aaatgaagtt ttaaaacatg tactatcaaa ctatgttgag gttcgtgtac
     7321 aaagggcact gcttgaaagt gtcacaagtg agcacggatc gagaatgact gctatggaaa
     7381 acgcaaccag aaatgcagac tcagtcatca ggagccttac actgcttttt aataaaacca
     7441 ggcaggcttc tattacgact gaacttatgg acattgtaaa cggtactgaa gctctaagaa
     7501 aaggaggagg tatggactaa tggaagccac cacacaaaac acaggaaaaa ttatacaggt
     7561 gatggggcct gtcgtagatg ttgaatttaa ggactcaagc cttcctgcaa tttatacagc
     7621 gctaagggtt acaaacaaac ttattagtga agaagactgg aacctggtat tggaagttgc
     7681 acagcagctt ggaagcaaac gcgtaagatg tatatctatg gactctactg aaggac
//
LOCUS       DBNGSV010000365         6890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1866491, whole genome shotgun sequence.
ACCESSION   DBNGSV010000365 DBNGSV010000000
VERSION     DBNGSV010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1866491"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="AC159U_12970"
     CDS             complement(<1..634)
                     /locus_tag="AC159U_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338007.1"
                     /translation="MNLKSLIRNSFGQLTKNKLRSTLTSIGIIIGVAAVILSVSITNS
                     AKILVETQFLSLGGKSLIINKGTRTKSGKVNEDFKIDFSIGDVQAIKNLNIIESASPV
                     SDDAQYVKFKDTSHLSAIVGASVDFFYVNNWFPIEGNLFTDKDVLNSENVCLIGKTVA
                     NRLFGFINPVGQRIKILNTNYEVLGVLSTIGQTSSGRDQDDAVIIPYTSYH"
     gene            complement(679..1347)
                     /locus_tag="AC159U_12975"
     CDS             complement(679..1347)
                     /locus_tag="AC159U_12975"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3338008.1"
                     /translation="MNKVIEIKKLYKDYLLGDVEVKVLRGIDLIVEQGDFLSIMGPSG
                     SGKSTLMNIIGCLDIPTSGQYLLDGTDISKLDSDSLAEIRNSRIGFIFQNFNLIPRTS
                     ALENVELPIIYSSNNQKEIRDRALQSLDKVGLSDRIDHTPAQLSGGQQQRVAIARAIV
                     NNPSIILADEPTGNLDSATSEDVLKILTDLNNSGKTIVMITHEDEVAAITKKTIRIRD
                     GQIV"
     gene            complement(1370..2530)
                     /locus_tag="AC159U_12980"
     CDS             complement(1370..2530)
                     /locus_tag="AC159U_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3338009.1"
                     /translation="MNIQNLAKNKYRLAAVVIAVIALFMLMGKDDDKPSFRTTRVQVG
                     DIKSYIQATGKIEPTRKAEISAEVTGIVNEVLVDYNSKVKKDQILATIDPTDFELKLS
                     EARANLAKANADFELSRNIYFSNKTLYNKNLISSEELKNSKVQYSSALASREQAQVNL
                     EQAKTDLANTKLQSPIEGSIIGKNIVEGQSVAKNKVGPPLFTVANNLDKMVVIASVNE
                     IDIGKVKEGNNCEFTTDAYPDQIYSGQITQIISEPITVNNLVSYDVLIEFENIDKKLK
                     PGMTASVEILVGSKNNVLIADRSALRFIPPSNEYIVNNAKYSQDDEILWVKARGGKLK
                     PIIITTGTKNDTHVEITGGEIKKDDEIVVLAVFGTKSTDSSGQISVPGVKRF"
     gene            complement(2527..3714)
                     /locus_tag="AC159U_12985"
     CDS             complement(2527..3714)
                     /locus_tag="AC159U_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3338010.1"
                     /translation="MNLDRSIKIAVENNLNIKVSDEDVNIMSARHKKSKSPLYPQIRF
                     RFILPFVERESGVFADQLLWDFWRTPSLVKASSASLRSSRHQRNATVNDVILNTKIAY
                     YNLLINQNIYETNRYEVEEFEKKLEQTENFVQLGRKSRLDLTKAKVDIGHAKLNLLNS
                     EKEFELSKANFMNLLALDGEFNFDLEDESDYEPYDWNLKELTKIALKNRPELKSLISD
                     ESSVRSSLKAAERDHYPKVVARAAYRFDGDGATGPDFIAGIGLNFPLFQGFAKVAEVD
                     ESRASLRKIMTEIEIFKRNTIMNIKRLYLDIKYAREKIGVTTDGLISAEQTMELTKEL
                     YKAGRKSMIDVLEAKSLYSRSKTDNLASIYNYKVALAKLEWATGEPLTNIDKDGDEIK
                     QTK"
     gene            3876..5036
                     /gene="hemW"
                     /locus_tag="AC159U_12990"
     CDS             3876..5036
                     /gene="hemW"
                     /locus_tag="AC159U_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3338011.1"
                     /translation="MNLALYIHIPYCITKCPYCDFNSYGVGADVPEDLYTDTLIKELF
                     IHKDLLAKFNISSIFFGGGTPSLFSAQNLEKILNTVSSLTKFTENTEITVEVNPKTAD
                     IDKFKSFRDIGINRLSIGIQSFSMRKLNFLGRLASPADNRQCIEQVISSGMENFNVDL
                     MYGTTAESYTEWEDDLLIATEYKPNHISAYCLTIENGTEFKKRFERGDLVLPKDDVLA
                     EMIDFTTGYLEDKGYNQYEISNYSKPGFECLHNKFYWQGKDYLGIGAGAHSHLRSDDS
                     SDWGKRWSNVRYPGRYIDLLRGGKSSVDSTESLTRIQAFEDDLMMGIRLMGGLDLDRL
                     NRRYNISFNLQDCDYLFSQSLITQSENSISLSKKGVLVSDYIISRLVDSAQF"
     gene            complement(5045..5497)
                     /locus_tag="AC159U_12995"
     CDS             complement(5045..5497)
                     /locus_tag="AC159U_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338012.1"
                     /translation="MNQKNSKKPRIFIDKEGNWFQDGLPVLHRWTYLYNNKLVDIDDE
                     GVYFIDEGRGRVYIEVEDTPFVIKNIDLRSDGLYLILNDETQEKLGSDTIYLNASNIP
                     YVKVKDGRFDARFCRAAYYEIMKMLETENEKYYLDTGSERIKVQRSTE"
     gene            complement(5564..6007)
                     /locus_tag="AC159U_13000"
     CDS             complement(5564..6007)
                     /locus_tag="AC159U_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338013.1"
                     /translation="MMKYILAITLTTLFILTTTSYSQKIYHWEKEGVDNYSNNPAEVP
                     YEQIKDNKISGKQTNESEAQAIAKLNETLDGPDKEKLRLILEAESKLEKVIAVKSKEL
                     ISLKKQKNVPAKKIVDLENYILKAEHNLKIIREEKTTFIKQNTNS"
     gene            6192..>6890
                     /gene="sucD"
                     /locus_tag="AC159U_13005"
     CDS             6192..>6890
                     /gene="sucD"
                     /locus_tag="AC159U_13005"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3338014.1"
                     /translation="MSILINSDTRLIVQGITGSAGLFHATQCRDYGTNVVGGVTPGKG
                     GTEVEGFTVFDTVDEAVRKVDANTSLIFVPAPFAMDAIIEAVDAGVELVICITEGVPT
                     LDMVKVKKYMEGKTARLIGPNCPGVITPGQAKAGIMPGYIHKPGRVGIISKSGTLTYE
                     AVWQLTELGIGQSTCVGIGGDPIIGSTFIDILTLFEKDKKTDAVIMIGEIGGNAEEEA
                     AAFIKDNFTKPVAAF"
BASE COUNT         1893 a         1283 c         1307 g         2407 t
ORIGIN      
        1 tatgatatga agtgtaggga ataattacag catcgtcctg atccctgccg ctgcttgtct
       61 gtccgattgt gcttagtaca cctaaaactt catagttggt gtttagtatc tttattctct
      121 gtccaaccgg atttataaat ccaaacagcc tgtttgcaac ggtctttcca ataagacata
      181 cattctcact gtttagtacg tctttatccg taaaaaggtt accctctatg gggaaccagt
      241 tgtttacata aaagaagtcc acggatgctc ccactattgc agagagatgg gaagtgtctt
      301 taaactttac atactgtgca tcatcggaaa caggcgaggc gctttcaata atatttagat
      361 ttttaattgc ctgtacgtca cctatactaa aatcaatctt aaaatcctca tttactttac
      421 cactttttgt cctcgtacct ttgtttatga ttaacgattt ccctccaagg ctcaaaaact
      481 gtgtttctac taaaattttt gcgctattgg ttattgatac ggatagtatt acggcggcta
      541 cgccaataat tattcctatc gaagtaagcg tagacctaag cttgtttttt gtaagctgcc
      601 caaaggagtt tctgattaag gattttagat tcatttagtt aactgttctc tttataaatg
      661 ttttgtatat gctgactatt atactatctg tccgtctctt attcttatcg tttttttggt
      721 tattgccgct acttcatcct catgggtaat catgactatt gtcttgccgc tattgttaag
      781 atcagttagg atttttaaga catcttcact cgtagcactg tcgaggtttc ctgttggctc
      841 gtcagcaaga ataattgagg gattgtttac tatggctctt gcgattgcta ccctttgctg
      901 ctgacctccg gaaagctgtg caggggtatg gtctatcctg tctgaaagac caaccttatc
      961 aagcgactgc agcgctctat cccttatttc tttttgatta ttagatgaat atataatagg
     1021 cagctcaaca ttttcaagag cgcttgtcct ggggattaga ttgaaattct ggaaaataaa
     1081 tcctatcctt gagtttctta tctctgcaag tgagtcactg tcaagctttg atatatcggt
     1141 tccatccaga aggtactgtc ctgaagtggg tatatcaaga cagcctataa tattcataag
     1201 tgtactcttt cccgagcctg atggacccat gatagacagg aaatcacctt gttctactat
     1261 aaggtctatt cccctaagca ctttaacctc tacgtctcct aagaggtaat ccttatagag
     1321 tttttttatt tcaattactt tattcaaggc agattttttt ttttaaagtc tagaatcttt
     1381 taacgccggg tacgcttatc tgtcccgagg agtcagttga tttggtgccg aaaacagcga
     1441 gtacaactat ttcatcatct ttttttattt ctccacctgt gatttctaca tgcgtatcgt
     1501 ttttggtgcc ggtagtgatt attataggct ttagtttgcc tcctctggct ttgacccata
     1561 gtatttcgtc atcctgggaa tatttggcat tatttactat gtactcgttt gatggtggaa
     1621 taaacctgag cgcagaacgg tctgctatta agacattatt tttgcttcca accagaattt
     1681 ctacgcttgc ggtcatgccg ggttttagtt ttttgtctat gttttcaaac tctatcaaaa
     1741 catcatagct tacaagattg ttaaccgtaa tgggctcgct tatgatttgc gtaatctgcc
     1801 ctgaatatat ttggtccggg taagcgtctg ttgtaaattc gcaattattg ccttctttga
     1861 ctttacctat atctatttca tttactgaag ctataaccac cattttatca agattattag
     1921 caactgtgaa aagaggcggg ccaaccttgt ttttagctac agactgacct tcaactatgt
     1981 tcttccctat aattgatccc tctattggag actgaagctt tgtgttggca aggtctgttt
     2041 ttgcctgctc gagatttacc tgtgcctgct cccttgatgc aagggcggag gagtattgaa
     2101 cctttgagtt tttaagttcc tccgaggata taaggttttt gttatatagg gttttatttg
     2161 aaaaatatat gttcctggac aactcaaaat cagcatttgc ctttgcaagg tttgctcttg
     2221 cttcacttag ctttaattca aaatcagtag ggtcaattgt tgcaagaatc tgatcttttt
     2281 ttactttaga attataatca acgaggacct catttactat gcctgtaacc tctgccgata
     2341 tttcggcttt tcttgtgggt tctattttcc ccgtggcctg aatatagctt tttatatcac
     2401 ctacttgtac tctcgtagtc ctaaagctgg gtttgtcatc gtcttttccc ataagcatga
     2461 aaagagcaat tactgctatt acaactgctg ccagtctgta tttatttttt gctagatttt
     2521 ggatattcat tttgtctgtt taatttcgtc tccatcttta tcaatgtttg tgagcggctc
     2581 gcctgttgcc cattcgagct ttgcaagcgc taccttataa ttatatatag atgctagatt
     2641 atcggtttta gatcttgaat ataatgattt agcttcaaga acgtctatca tggacttcct
     2701 tcctgcttta tataactcct tcgtaagctc cattgtctgc tctgctgata ttagtccatc
     2761 tgtcgttacc cctatttttt ctcttgcgta tttaatatcc aggtagagtc tttttatatt
     2821 cattatagtg ttgcgcttaa atatttctat ttcagtcatg atttttctaa gtgatgcccg
     2881 cgattcatcc acctcagcaa ctttggcaaa accctgaaac agtgggaaat taaggccgat
     2941 accagctata aaatcaggtc cagttgctcc gtcaccgtca aacctgtagg cagcccttgc
     3001 aacgactttg gggtaatgat ccctttctgc ggcttttagt gaagaacgaa ctgaagattc
     3061 atcgctaatg aggcttttta gttcaggcct gttttttagc gctattttag taagctcttt
     3121 taggttccag tcatatggct catagtctga ttcatcttct aaatcaaaat taaattctcc
     3181 atccaacgca agcaggttca tgaaatttgc ttttgataat tcaaactctt tttcagagtt
     3241 tagcaggttt aattttgcat gacctatatc aacttttgcc tttgtaagat caagtctgga
     3301 ctttcttcca agctgtacaa aattttctgt ctgttcgagt ttcttttcaa attcttcaac
     3361 ctcatacctg ttggtttcat atatgttctg attaatgaga agattgtaat acgcaatctt
     3421 ggtatttagt attacatcgt ttacagttgc atttctctgg tgtcttgaag accgaagtga
     3481 tgcactgctt gcttttacaa gactcggtgt acgccagaaa tcccagagta actgatcggc
     3541 aaatactcca gactctcttt caacaaacgg aagaataaac ctgaatctta tctgagggta
     3601 aaggggggat ttggattttt tgtgcctggc agacattatg ttcacgtctt catcagacac
     3661 tttgatattg agattgtttt ctacagcgat tttaatcgat ctatcaaggt tcaatatctg
     3721 agagccttgc tcaccaaaaa ctctaaaggt acatataaaa aatagtaaga atattgccga
     3781 atataatctg ctcatgtcat taagatagtt taaacaataa atatgactca aattgtttta
     3841 cttgcaacta aatctgtttc taatacaatc ttaatatgaa tttagcgctt tatatacata
     3901 taccgtattg tattaccaaa tgtccatact gtgattttaa ttcctatgga gtaggtgcag
     3961 atgtgcctga agacctttat acagatacac tcataaagga actgtttata cataaggacc
     4021 tccttgctaa atttaatatc agctctatat tttttggggg aggaacacct tcactctttt
     4081 cggcacaaaa tctcgagaaa atattaaaca ctgtctcatc gctgactaaa tttaccgaaa
     4141 atactgaaat caccgtagag gttaatccaa agactgcaga tattgataaa tttaagagtt
     4201 ttagggatat tggaataaac aggctgagta ttggtataca gtccttttca atgagaaaat
     4261 taaattttct gggcagactc gcaagcccag ctgataatag acagtgtata gagcaggtta
     4321 ttagctctgg aatggaaaat ttcaatgttg accttatgta tggcactacc gctgaatctt
     4381 atacagagtg ggaggatgat ctactgattg ctacagagta caagcccaat catatttctg
     4441 cttactgtct tacaatagaa aacggaactg aatttaaaaa gagatttgaa agaggcgatc
     4501 ttgtattgcc aaaagatgat gtgcttgcgg agatgataga ttttactaca ggttatttgg
     4561 aggataaggg ctataaccag tatgaaattt caaactactc aaaacctgga tttgagtgtt
     4621 tgcacaacaa gttttactgg cagggaaagg actatctggg aataggagca ggggctcatt
     4681 ctcatttgag aagcgatgat agctcggact ggggaaagag atggtcaaat gtgaggtatc
     4741 ccggcagata tattgactta cttcgcggtg gtaaatcttc agttgatagt accgagtcgc
     4801 ttacaagaat acaggcgttt gaggatgatc ttatgatggg tataaggctt atgggtgggc
     4861 ttgatctaga tagattgaac aggagatata atattagttt taatttgcag gactgtgatt
     4921 atctgttttc tcaaagtctg attacacaat ctgaaaatag tataagcctt agtaaaaagg
     4981 gagtacttgt ttctgattat ataatttcaa gactcgtgga cagcgctcag ttttagaaac
     5041 caccttattc tgtacttcgc tgcactttta ttctttcact gcctgtatca agataatatt
     5101 tttcattttc agtctcaagc attttcatga tttcataata agcggctctg caaaagcggg
     5161 catcaaacct gccatcctta acttttacat aagggatatt cgaggcgttt agataaatag
     5221 tgtccgatcc cagtttttcc tgagtctcat catttaatat cagataaagc ccatctgatc
     5281 tgagatctat attttttatg acaaacgggg tgtcctctac ctctatatat actctgccac
     5341 ggccttcatc tataaaataa acgccttcat cgtcgatatc cacaagcttg ttgttgtaaa
     5401 gataagtcca tctatgcagc accggcagtc catcctgaaa ccagttgcct tctttatcta
     5461 tgaatatccg tggtttttta gaattcttct gattcacctg ttagagaaac actgagtgtt
     5521 gttgatatat atttttaagt ttagagaatg cagaagaatt gacttaagaa tttgtattct
     5581 gcttaataaa tgtggttttc tcttctctaa tgatcttgag attatgctct gcttttaaaa
     5641 tatagttttc aagatcgaca atttttttag ctggaacatt tttttgcttt tttagactta
     5701 taagctcttt actcttaact gcgataacct tttcaagctt ggattcggct tctagaatta
     5761 atctgagttt ttctttgtcg ggaccatcca gtgtttcatt taatttagca attgcctgag
     5821 cttctgattc atttgtttgt tttccggata tcttattatc ttttatctgt tcataaggta
     5881 cttctgcagg attattagaa taattatcta ccccttcttt ttcccagtga tatatttttt
     5941 gagaataaga tgtggtagtt aatataaata aggttgtaag tgtaattgcc agtatatatt
     6001 tcatcatctc tttattataa aacgttgttt gtattttgcc aaatcgattt ataaaattat
     6061 ttatattaat taaactataa taaatcctga tttgtttcaa aatattcata atctcagtag
     6121 ttgaaagatt taagtcctat gttatcattc cagccgtttt aatataattt tcaccaataa
     6181 agaggagatt tatgagcatt cttattaata gtgatacaag acttatagtt cagggaatta
     6241 caggcagcgc agggcttttt catgcaacac agtgcagaga ctatggaact aatgtagtag
     6301 gcggtgtaac acccggcaag ggaggcaccg aggtcgaagg atttacggtg ttcgataccg
     6361 tggatgaagc cgtaaggaag gttgatgcca atacttctct aatatttgtt cctgccccat
     6421 ttgcaatgga cgctataatt gaggctgtag acgcaggagt tgagcttgta atctgtatta
     6481 cagagggtgt tcccactttg gatatggtaa aggtgaaaaa atatatggaa ggcaaaaccg
     6541 caagactcat aggtccaaat tgccctggag taatcactcc gggacaggca aaggcaggga
     6601 tcatgccggg atatattcat aagccgggca gggtaggcat tatctccaag agcggaacat
     6661 taacctatga ggctgtatgg cagcttacag agcttggaat tggtcagtcc acttgtgtgg
     6721 gcattggcgg agatcctatc ataggctcga cttttataga tatacttaca cttttcgaaa
     6781 aagataaaaa aacagatgct gtaattatga tcggtgagat tggcggaaat gctgaagaag
     6841 aggcagcagc atttattaaa gataatttca caaaaccagt agccgctttt
//
LOCUS       DBNGSV010000366         7018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_551403, whole genome shotgun sequence.
ACCESSION   DBNGSV010000366 DBNGSV010000000
VERSION     DBNGSV010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_551403"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1992
                     /locus_tag="AC159U_13010"
     CDS             100..1992
                     /locus_tag="AC159U_13010"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HIR3338015.1"
                     /translation="MSTKKTLIISTVALTIVTALVINTSAHIVKEQPWHPLPASYLRS
                     VFYLNLKPINWELVEKEYSSQAGSGYDYRSVYEILDQITKFSNKNYQAEIKEAISSKN
                     KEKVYQITTNALSNYIRFLLTKAQQEIDNPSKASQYISNAKELYRAFEKFIHKYDEQN
                     YVILGKAWLNMNTSLGSRGVLGAAEIKPDTATFTKSKKAIEDYLIANYEKSVRKNVLI
                     PVPLSVDKNDTNKSDFDWLPPGTNLNDQVPLPRLVLNFEERGFDEKDLFLVAYGDMLF
                     DSKEIFGSPANDLGIACSTCHNRSDINKSFFIPGISHKPGSIDVDGQFFNPLFNDHRN
                     DSIDIPSLRGIRFTAPYGRDGRFASLRDFVRNVIVNEFGGAEPTPLMLDALVTYMLEF
                     DWLPSQYLNPDGTLNENASKQARNGEKLFNKKFHSMGGRACSSCHIPSSNFIDGLRHD
                     IGSGNASSPNARDSFFDTPTLINIKYTAPYFHDGSLENLSDVVEWFDNKYKLKLNEKE
                     KSDLTAYLDEVGTGEEPFENFDYENTQFTLDWSELSTFLSTLNTLIPAEDKFHIALLL
                     NTVSKDLKFDAAGLKNLDQSSLVYELTDKLDNILDAVEKNDWPTSASLWLEYKELENK
                     YGPKFK"
     gene            2006..2893
                     /locus_tag="AC159U_13015"
     CDS             2006..2893
                     /locus_tag="AC159U_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3338016.1"
                     /translation="MKRQLKLSLIITLAAVILTGLNTYADLKENNNQDTLTLAFIPQE
                     NPEKLLGDIKQITKYLEKEIGVTVKGYVTQDHAAAVEALRSGQADVSFMGGLPYVLAH
                     NEIGAEVLLSEVYRGSPLYKGRIFVRRDSGINSLKNLKGKSIAFADPISESGYLYPLD
                     LFVKQGFLEQGEDANNFFKTVYFAGGYQQAIQALANGLVDAAGVSQFADLLLTAEQLA
                     KVKWIAESEDIPSHVVCSRKGLNPVIKENFKKAMLKLNSSEYSHLLKYVYSPDGYVEA
                     THKNYEHVEQMAKLYGYLK"
     gene            2923..3720
                     /locus_tag="AC159U_13020"
     CDS             2923..3720
                     /locus_tag="AC159U_13020"
                     /EC_number="7.3.2.2"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR3338017.1"
                     /translation="MEVYIGQDKNSVSRLYLQKPDVVIRKLAVRYPGENSDVLDIDNL
                     IIPKAQTVGIIGSSGAGKTTLLRTIGGFIEPYFGSVDVSDNKRGNIGFIFQNFNLIGR
                     STVFENVLFGRLGKTNRIKSLLGMFSQKDKDLAYEAIKEVNLLDKINQRTDTLSGGEM
                     QRTAIARVITQQAGVILADEPVSNLDPALSHEILDLLVETSTKHGATLIINLHQPALA
                     KRYVQRIIGLRKGSVVFDAESAELDNSHLNLIYETEFESSIMFDTKS"
     gene            3732..4517
                     /gene="phnE"
                     /locus_tag="AC159U_13025"
     CDS             3732..4517
                     /gene="phnE"
                     /locus_tag="AC159U_13025"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR3338018.1"
                     /translation="MNTKDSRSIKPAFTIQRIVTILVLVFLFFWSSKGADFQTSKLIE
                     GLPRMAEFFSRMVPPDMSVAQTVFKSTIETIQIALFGTFLSAIMSFFAGILAAENITP
                     AYINRPVKWLLAMLRGIPVILLALLFVSTVGLGPFPGVLAIALHSTGMLGKFYSEAME
                     NAKIGPIEALQATGANFAQKIRFGILTQVAPDLVRDTLFRFELNLRESLILGLVGAGG
                     IGFYILLYIRSFQYEKVATLTLVVLIAVVIIEQISISIRKSLR"
     gene            4610..5380
                     /locus_tag="AC159U_13030"
     CDS             4610..5380
                     /locus_tag="AC159U_13030"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3338019.1"
                     /translation="MKIKILGCSGARSTKSNPTCFLIDDKILIDAGSVTNNLEDSAIR
                     NIDHLLITHSHFDHIADLPFLLEKLFWIKESPFTIYSSSESWNTIKSHILNNSVWPNL
                     IEIAKSKNELFKWKEFSSLNSFKLSNYEVTPVRVNHIVPTDGLIIDNGTCSFAFTADT
                     YKTDQFWEQCKNQQNLKAIIAGVSFPNHMYETAEITRHLTPEFFHDEVKRLGRDDIKY
                     YISHIKPLFQDEVLKDIEKLPIQENIEILEDGKLIELD"
     gene            complement(5393..6253)
                     /locus_tag="AC159U_13035"
     CDS             complement(5393..6253)
                     /locus_tag="AC159U_13035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3338020.1"
                     /translation="MRFLILFLCVLIFSSCSAKKVLKAPERSLNRISAAVENSKGITP
                     DQEYYIGRAVAAKIVSTYSILEDEELTKYLNLVGNTVAENSDNPDIPAGYHFGVLDTK
                     DINAFACPGGVVFVTKGLIDTLESEDELAAVLAHEIAHINNRDGILSIEDARKSGVLS
                     AILLEPFRFAASLTPLHLSSFSGAFLSSVDSVTNTVSEKGYNKDQEFAADSDALKFLQ
                     RAAYDPSALINYQKKVLEHHNKVESEDLQTHPGLAERINILKSIAPASEQDDAKVELR
                     KKRFNKALKG"
     gene            complement(6358..6810)
                     /locus_tag="AC159U_13040"
     CDS             complement(6358..6810)
                     /locus_tag="AC159U_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338021.1"
                     /translation="MARFLLFLSVLLVGAGFSYAEQVKVLSSVNAIREECKFFSPVKA
                     KVNYNDELSILDKKGDWNWVSFLDVQGCIHNSAITERKVALTNLDTSSDDIVSYNEVS
                     LAGKGFFQELYTFTNPNAKFGTLEAIESFEVTEQQLAEFAQEGSLHLP"
BASE COUNT         2383 a         1287 c         1308 g         2040 t
ORIGIN      
        1 aatcgaaaat atagtaggcc agagggaatt tggaatacat ggggaaaaaa tccccgtatt
       61 aaattaattt aaggaaaatt aagtacaact aaaaaaaaga tgagcacaaa aaaaacatta
      121 attataagca cagtagcact aacaatagtt acagcacttg ttattaacac gagtgctcat
      181 attgttaaag aacagccgtg gcacccgctg ccggcttctt atttaagatc tgttttctat
      241 ctcaatctaa aacccattaa ctgggaactt gtagaaaaag agtacagctc tcaggcaggg
      301 tccggctacg attacagatc agtttatgaa atactcgacc agatcacaaa gtttagcaac
      361 aaaaactatc aggcagaaat taaagaagct ataagttcca aaaacaaaga aaaagtttat
      421 caaattacaa ctaatgctct ttccaattac attagatttt tactgacaaa agctcagcag
      481 gagattgata atccttcaaa ggcttctcag tacataagca atgcaaaaga gctatacaga
      541 gcgtttgaga agttcattca taaatatgac gagcaaaact atgtgattct tggtaaagcc
      601 tggctcaata tgaatacgag cctgggatcg agaggagtac ttggcgctgc cgagataaaa
      661 cccgatacag ccacatttac caaatctaag aaggctatag aggattacct tatagctaac
      721 tatgaaaagt cagtaagaaa gaacgtactc atacctgttc ctcttagtgt tgataaaaat
      781 gatacgaata aatcagattt tgactggctt cctcccggaa ccaacttaaa tgatcaggtt
      841 ccactgccaa gacttgtact taattttgaa gaacgaggat ttgatgaaaa agacctgttt
      901 ttggtagcct atggagatat gctttttgat tccaaagaaa ttttcggaag ccctgcaaac
      961 gatcttggca tcgcatgttc tacgtgtcat aacagaagcg acataaacaa aagttttttt
     1021 attcccggaa taagccacaa accgggctca atagacgtgg acgggcaatt ctttaaccct
     1081 ctctttaatg atcacaggaa tgactcaata gatattccaa gtcttagggg aataagattc
     1141 acagcaccct atggaaggga cggcaggttt gcaagcctta gagactttgt aagaaatgta
     1201 atagtgaacg aatttggagg ggccgagcct accccgctta tgctggatgc gcttgttacc
     1261 tatatgctcg agtttgactg gcttccatca cagtatctaa accctgacgg tactctcaat
     1321 gaaaatgctt ctaaacaggc cagaaatggt gaaaaattat ttaataagaa gtttcattca
     1381 atgggaggga gagcttgcag cagctgccac attccaagtt ctaactttat agacggtctc
     1441 aggcatgata taggctctgg taatgcatcc tcaccaaatg caagggacag ctttttcgat
     1501 actcccacgc ttataaatat taagtacact gcaccttatt ttcatgatgg gtcgcttgaa
     1561 aacctttcag atgtggtgga gtggtttgat aataaataca aactcaaatt aaatgaaaaa
     1621 gaaaaatccg atctcacggc atatcttgat gaggttggca caggggaaga accgtttgaa
     1681 aattttgatt atgaaaacac acagttcacc ctggactggt ctgagctttc tacattttta
     1741 agcacgctaa acacactcat acctgctgag gataaatttc atattgccct gctcttaaac
     1801 actgtatcaa aagacctgaa atttgatgca gcggggctta aaaatctgga tcagtcttca
     1861 cttgtgtatg aacttacaga caaactggac aatatattgg atgccgtaga aaaaaatgac
     1921 tggccgacat ctgcatcttt atggctcgaa tacaaagagc ttgaaaacaa gtatggacct
     1981 aaattcaaat agcaaagagg ataaaatgaa aagacaatta aaattaagtt taataataac
     2041 attagcggct gtgattttaa cagggctaaa tacttatgca gacctgaaag aaaacaacaa
     2101 tcaggataca ctgacccttg cattcatacc acaggaaaat cccgaaaagc tacttggtga
     2161 tattaaacaa attacaaaat atcttgaaaa agaaataggc gttactgtaa aaggatatgt
     2221 aacacaggat catgcagcag cggttgaggc gcttaggagc ggacaggccg atgtttcatt
     2281 tatgggtggt ctgccttatg tccttgctca caatgaaata ggtgcagagg tattgctctc
     2341 tgaagtgtac aggggaagcc ctctatataa aggaagaatt tttgtacgaa gggacagcgg
     2401 gattaacagc ttaaagaatc tcaagggtaa aagcatagcc tttgccgatc ccatatctga
     2461 atcaggatac ctgtatccgc tagatctatt tgttaaacag ggatttttag agcagggcga
     2521 agacgctaat aattttttta aaaccgtata ctttgctggc ggctatcagc aggcaataca
     2581 ggctttggca aacgggcttg tagacgctgc cggagtaagt cagtttgctg accttcttct
     2641 cactgcagaa cagcttgcaa aagtaaagtg gatagccgag tcagaggaca tcccttctca
     2701 tgtggtttgt tcaaggaaag gacttaatcc tgtaatcaaa gaaaatttca aaaaagcgat
     2761 gcttaagtta aacagttcag aatatagcca tttacttaaa tatgtttaca gcccggatgg
     2821 atatgtggaa gctacacata aaaattacga gcatgttgag cagatggcaa agttatatgg
     2881 ttatttaaaa taacaggaat cttaaaagcg aggataaatg aaatggaagt ttacattgga
     2941 caggataaaa actcagttag tcgtctgtac ttacaaaaac ctgatgtggt aataagaaaa
     3001 ttagccgtca gatacccggg tgaaaacagc gatgtactgg acattgataa tctcataatt
     3061 cccaaagcgc agacagttgg aataataggt tctagtggtg caggtaagac tacattactc
     3121 cgtactattg gtggttttat tgaaccctac tttggctcag tagatgtatc agataataaa
     3181 aggggcaaca ttgggtttat atttcaaaac tttaacctaa ttggaagaag tactgtattt
     3241 gaaaatgtac tgtttggcag gttagggaag acaaacagaa ttaagagttt actgggaatg
     3301 ttctctcaaa aagataaaga tcttgcatat gaagcaataa aagaagtaaa tttattagat
     3361 aagataaatc agagaaccga tacactaagt ggaggagaaa tgcagagaac cgcaattgca
     3421 agggtgatta cccagcaggc gggggtaata cttgcagacg aacctgtgag caatctagac
     3481 cctgcacttt ctcatgaaat tttagacctg cttgtagaaa caagcacaaa gcacggagca
     3541 actcttatta ttaatcttca ccagcccgca cttgcaaaaa gatatgtaca aagaattatc
     3601 ggccttagaa agggaagtgt agtttttgat gccgagtcgg cagagcttga taactcccat
     3661 cttaacttaa tttatgaaac agaattcgaa tcatctatta tgttcgatac taaaagttaa
     3721 ataacagtaa tgtgaacaca aaagactccc gctcgattaa gcctgcattt accatacaaa
     3781 gaattgtaac catacttgta cttgtatttc tgttcttctg gtcatcgaaa ggcgctgatt
     3841 ttcagacctc taagcttatt gaaggtttgc caaggatggc cgaatttttt tcccgcatgg
     3901 ttcctcctga tatgagcgta gctcagactg tattcaaatc tactatcgag actatacaga
     3961 tagcgctgtt tggaacattc ttaagtgcaa taatgtcttt ctttgctggt attctcgcag
     4021 cagaaaatat tactccagca tatataaaca gaccggtaaa atggcttctt gcaatgttaa
     4081 gaggcatacc ggtaatcctt cttgcgcttt tatttgtaag cactgtagga ctgggacctt
     4141 ttccaggggt acttgcaatt gcgcttcatt ccactggtat gctcggcaaa ttttattctg
     4201 aagctatgga aaatgccaag ataggaccta ttgaagcact tcaggcaaca ggggctaact
     4261 ttgcacagaa aattagattt ggtattttaa ctcaggtagc tccagatctt gtacgggata
     4321 ctctatttag gtttgagcta aatctcaggg aatctcttat tctgggcctt gtaggtgcag
     4381 gaggtattgg gttttatata ttattgtata tccgttcatt tcagtacgaa aaagtggcta
     4441 cacttacact tgtagttctc attgctgtag ttattataga gcaaataagt atatctataa
     4501 gaaaaagttt aagataggcc atcttgaatt tattactcaa agaatataat atatagattc
     4561 attactatca tataacttca acttatctta ataaacatat tattctgtta tgaaaatcaa
     4621 gatattgggt tgttctggtg caagaagcac caaaagtaat cctacatgtt ttttaattga
     4681 tgataagatt ttaatcgatg ctggatccgt aacaaataat cttgaagact cagctatcag
     4741 aaacatcgat catcttctaa ttactcactc acactttgat catatagctg acctgccttt
     4801 tttacttgaa aagcttttct ggatcaaaga gagtccattt actatctact catcctcgga
     4861 gagctggaat acaataaaat cacatatact taataacagc gtatggccaa acctgataga
     4921 aatagcaaag agtaaaaatg aactatttaa atggaaggaa ttttcaagct taaattcatt
     4981 taaactatca aattacgaag ttacacccgt tcgcgtaaac catatagtac caacagatgg
     5041 gttgatcatt gataacggga cgtgctcctt tgcttttacg gcagatacat ataaaacaga
     5101 ccaattctgg gaacaatgca aaaatcagca aaacttaaaa gcaattatag ccggagttag
     5161 ttttcccaac catatgtatg aaacagctga aatcacaaga catctaaccc cagaattttt
     5221 ccatgatgaa gttaaaagac tcggccggga tgatattaaa tattatatct cacatataaa
     5281 acctctgttt caagatgaag ttcttaaaga tatagagaag cttcctatac aggaaaacat
     5341 tgaaatatta gaagatggaa aactgataga attagactag aaggttagag tatcagcctt
     5401 ttaaagcttt attaaatctt ttcttcctga gctcaacttt tgcatcatcc tgctctgaag
     5461 caggagcaat agatttaagt atgtttattc tttcagcaag tccggggtgt gtctgcaaat
     5521 cttctgattc tactttattg tgatgctcta gtaccttttt ttgataatta atcaaagctg
     5581 acggatcata tgctgccctc tgtaaaaact ttaaagcatc actgtctgca gcaaattcct
     5641 gatccttatt atatcctttt tcagatactg tatttgttac tgaatctact gaagataaga
     5701 acgcaccgga aaaactagag agatgaagag gtgtaagact tgctgcaaat ctaaatggtt
     5761 ctaataaaat tgcagaaagt acacctgatt ttctagcatc ttctatagac aatataccat
     5821 cacgattatt gatatgcgca atttcgtgag ccagaaccgc tgcaagctca tcctctgatt
     5881 caagtgtatc aatcaggcct tttgttacaa acactactcc acctggacat gcaaaagcat
     5941 taatgtcttt tgtatctaaa acaccaaaat ggtatccagc agggatatca ggattatcag
     6001 aattttcggc aacggtattt cctaccagat tgagatattt tgttaattct tcatcttcga
     6061 ggatagaata agtagatact attttagctg caactgccct gccaatataa tattcctgat
     6121 caggtgttat acctttagaa ttttctacag cagccgaaat acggtttaaa gaacgttccg
     6181 gagcttttaa aaccttcttt gctgaacagc tggaaaaaat taatacacaa agaaataata
     6241 tcaaaaatct cataattatc ttgttaatac cccaaatgca ttaatataaa aaacccctaa
     6301 gtctgtaatt ttacagaata tacttgagtc ttctgatttt actctaaaaa agaagaatta
     6361 aggtaagtgt aaacttcctt cctgagcaaa ttctgcaagc tgctgctctg ttacttcgaa
     6421 gctctcaata gcttccaatg taccaaattt ggcattggga ttagtaaaag tatatagttc
     6481 ctgaaaaaaa cctttaccag ctagtgatac ctcgttgtaa gatacaatat catctgatga
     6541 agtatcgaga tttgtaagag ctacttttct ctcagtgata gcagaattat gaatacagcc
     6601 ttgtacatct aaaaaactca cccagttcca atcacctttt ttatccagaa tgctaagttc
     6661 atcattgtaa ttcacctttg ccttcacagg tgaaaagaat ttacattctt ctcttatagc
     6721 atttacactt gatagaacct taacttgttc tgcatatgaa aaaccagccc caaccagtaa
     6781 aacagataaa aatagtaaaa atctggccat ttttccctcc taaaaccttt tttaccctac
     6841 tctcttaatt tttataatta ttatatctaa ttttaaagac ttatgcctac ccttgtaaat
     6901 gctcagtaat taagtaacca aattagtata tattaataac taaggaggtg cttaatgaaa
     6961 taccatgaga tagtggtagt aaagagatgg ataccaatac tcaaagaata cgagagaa
//
LOCUS       DBNGSV010000367         8059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_898407, whole genome shotgun sequence.
ACCESSION   DBNGSV010000367 DBNGSV010000000
VERSION     DBNGSV010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_898407"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="AC159U_13045"
     CDS             <1..119
                     /locus_tag="AC159U_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR3338022.1"
                     /translation="ELKQELLEYLVYYNHQRPHQSLGGVPPVEFLKNCPRIT"
     gene            346..1029
                     /locus_tag="AC159U_13050"
     CDS             346..1029
                     /locus_tag="AC159U_13050"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3338023.1"
                     /translation="MKTIKYIAGALIAILLIITTVVLFNTLKSVPTYESTKKIDIKSI
                     DHQGAVNRFAKSLMFKTIANYDDFPKSEYKRFHRFLENSFPLVHSTLKKEIVGGYSLL
                     YTWKGTDSSKKPILFMGHQDVVPIAPGTLGLWTHPPFDGKAVDGFIWGRGAIDNKSGV
                     MGIFEAVETLISDGFTPKQTIYLSFGHDEEIGGNEGAAKTVELLKSRGVKLDYVLDEG
                     GIITNGMFP"
     gene            1042..1812
                     /locus_tag="AC159U_13055"
     CDS             1042..1812
                     /locus_tag="AC159U_13055"
                     /inference="COORDINATES: protein motif:HMM:NF019307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3338024.1"
                     /translation="MAVIGIAEKGYISIELIVEGKGGHSSMPPKQTTVGILSNAITKL
                     ESNPFKPRLDGAAKKMFKKLTPHMPFYERMGLANLWIFECFALNRITKSQSANAMVRT
                     TTAPTMLQGSNKENVLAAKAKAVVNFRVLPGDTTETVIKRVTKVIDDKRVIINPGTLQ
                     INPSPISDINTSFYKVLEKSIRQHTEGNVITAPYLVVGATDSRHFIPLTKNIYKFIGI
                     TLSSSDLKGFHGTNEKLSADEYIRAVKIYYQIIKNINK"
     gene            complement(1817..2467)
                     /locus_tag="AC159U_13060"
     CDS             complement(1817..2467)
                     /locus_tag="AC159U_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338025.1"
                     /translation="MIKKYVWLQLALLFLFGTSIAYSIPKSAENIANAYGIEEFNKVI
                     KISYTFNVHINDKDIQRKWIWYPKSGHVIYVNENIKYRTNNVTDDLEDTDHKFINDKY
                     WLLFPFHLLWDENVEFTEADGKQSSPINNESLNKLEVKYVNDAGYTPNDIYELYYDDD
                     FIIKEWVFRKGGSVDNKKAMTWEQNKSYSGITISENHVGANDFRLWFTDVTVETEK"
     gene            2796..3386
                     /locus_tag="AC159U_13065"
     CDS             2796..3386
                     /locus_tag="AC159U_13065"
                     /inference="COORDINATES: protein motif:HMM:NF047071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3338026.1"
                     /translation="MTTKEKLIDSAIQLLGTRSYNSVGVQELCEHAEVKKGSFYHFFP
                     SKRDLTIVALDTIWENFKTHMLLPVLESDLEPLEKMKTLIDNGYKHLVDSKSCTGCIS
                     GCNIGNLAVELSTQDEEIRLKIASIFDEWTRLIEVLIQEAISKGQISCKVNVRASAQS
                     LLAFVEGALLVCKTYNDPEIMKTMCDNAFNMLMVRK"
     gene            3443..4132
                     /locus_tag="AC159U_13070"
     CDS             3443..4132
                     /locus_tag="AC159U_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3338027.1"
                     /translation="MLRQPIFIDNKVIPILSGNIEYFGEELNYKDLKDEQLASLYVKN
                     RDDNAYNEIVNRYGEKIFRLAMRITKNPESAEEVLQNVFVKLIEKLGTFREESKLATW
                     IYTVSSNEAFMYLRGKNKNSSKEISVEEFNNNDSGSYYEGLQLKYDGFGPDESVNNME
                     QQEILDKAIGELPEEYRVVFQLRDVEGLSNQQTADILGLSLPAVKSRILRARNQLKKR
                     LAEYFPEYKDS"
     gene            complement(4158..4475)
                     /gene="grxD"
                     /locus_tag="AC159U_13075"
     CDS             complement(4158..4475)
                     /gene="grxD"
                     /locus_tag="AC159U_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR3338028.1"
                     /translation="MSDIQQSIKKQIDDNKVILFMKGNKQAPQCGFSAQVVQILNSYD
                     VPYETVDVLLDPEIRQGIKDFSNWPTIPQLYVNGEFIGGCDICTEMFQNGELETVVKS
                     AAE"
     gene            complement(4497..4736)
                     /locus_tag="AC159U_13080"
     CDS             complement(4497..4736)
                     /locus_tag="AC159U_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR3338029.1"
                     /translation="MDPEKVKELIENGMNAEHVAVEGDGAHFEAVIVSDLFEGKTLIQ
                     RHQVVYRALGDAMKEDIHALSMKTYTPQQWQDMRN"
     gene            complement(4758..5165)
                     /locus_tag="AC159U_13085"
     CDS             complement(4758..5165)
                     /locus_tag="AC159U_13085"
                     /inference="COORDINATES: protein motif:HMM:NF022479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR3338030.1"
                     /translation="MDSAELWLTPLLLVPGVALLVMSTSSRFAQVHSEIHDLASHKHD
                     HDIYKLAMNLDKRAVIFRNALVSLYLSITFLAIASLVGGIAVIWLGDLFYFVMGLSCL
                     GIVCLVFAAIELIRESMITLEVIKEHCHQIEKE"
     gene            5341..6156
                     /locus_tag="AC159U_13090"
     CDS             5341..6156
                     /locus_tag="AC159U_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3338031.1"
                     /translation="MNKLILALLLLPVLFSACSKEEEPKYTEKDLQSQHHFAKKEAAK
                     KALVKRPKSTRNPVAVVDGIEIYAEDLENGNIDLTVKNEVLYQVGVEKGFDKVVEPKV
                     TKYERNLVLKMIKDELRRGIEPKVTDQETVDYYNNHKHEYTLVEVEELITATRDLAEE
                     VRKEALKGTDFKQIVQLNTTEQGATVAFNQTSNVVRVFNEAVDLGQVSQVINSGSQFI
                     VQKVINKSEPGFDDLKDTIAIFLIKEKADNEYESIIEKLKEEKNVEILAEAEK"
     gene            6306..7349
                     /gene="argC"
                     /locus_tag="AC159U_13095"
     CDS             6306..7349
                     /gene="argC"
                     /locus_tag="AC159U_13095"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3338032.1"
                     /translation="MAKKINVAILGASGYTGGELLRFLVNHPPVEITHLTADRSVGKN
                     LSDVLPTFRDILDIKLHPLQLDRIPKNIDFAFTALPHGTSGNVVKHLYEKGIKVVDLG
                     ADFRISHNVYKKWYGEHPCPELIDKAVYGLPELHRAKIKRAKIIANPGCYPTSSTLPL
                     APLLKNNLIKQNTIIIDSKSGVSGAGRSAALDYNFCEVNEGLKAYKIGEHRHMPEITE
                     VLSKYSHKRVLLNFTPHLIPMDRGILSTIYVDLNKKMETTDLLDVYKKFYRIEKFVRI
                     MPEGVYPSTHQVRGSNFCDIGCKVIPAQNKAILVSVIDNLVKGASGQAVQNMNLMMGY
                     PEDTGINLVPVFP"
     gene            complement(7346..>8059)
                     /locus_tag="AC159U_13100"
     CDS             complement(7346..>8059)
                     /locus_tag="AC159U_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3338033.1"
                     /translation="IYANNETGVIFPLDQISEITKEQGVLLHTDAVQAVGKIEINLKE
                     LPVDLLSLSSHKLYGPKGAGALYMRKGVDLSPLIHGGGQERGKRSGTENVPAIAGFGK
                     AAELIIDDIEAENNRLSALRDKLHKKITNSIDGVSLNGHLENRLSNTLNLNFDNTEGE
                     SLVMNLDIEGVAVSTGSACSEGNVDPSHVLLAMGLTKEQAISSLRFSFGRFSKESDID
                     LVLDKLPAIVERIRYINKT"
BASE COUNT         2702 a         1473 c         1519 g         2365 t
ORIGIN      
        1 aggaattaaa acaggaactg cttgagtacc tggtgtatta caatcaccaa agacctcacc
       61 agtctctggg gggagtacca ccagtagagt tcctaaaaaa ctgtcctcga attacgtgac
      121 atctacagca atgacaggga gggacccagc atccttcagg gacgggttaa aaacgttaac
      181 aacacattaa ggacttacga actttttcta ttccaaaaag cccgttctca cgatgacgga
      241 gaggggaggt acattaaagt tactgttatt aaattattga taatctatga tattaaagtt
      301 aaatctaatg caaactaatt tctacaagtt acgactaaca ataatatgaa aacaataaaa
      361 tatattgcag gagctttgat agcaatactg ctcatcatta ctacagttgt tttgtttaac
      421 actcttaaat cagtaccaac ctatgagagc acaaaaaaaa ttgatattaa aagcatagac
      481 catcagggag cggtaaacag gtttgcaaag tctctaatgt ttaaaacaat tgcaaattat
      541 gacgacttcc caaaatcaga gtacaagaga tttcacagat ttttggaaaa ctcgttcccc
      601 cttgttcatt ccacactaaa aaaagaaatt gtgggaggct acagcctgct ttacacatgg
      661 aagggaacag acagtagtaa aaagccgatc ctttttatgg gtcatcagga tgtggtaccg
      721 atagcaccgg gaactctcgg tctttggaca cacccgccat ttgacggaaa agcagtagac
      781 ggctttatat ggggcagggg tgccatagac aataagtccg gtgtgatggg tatttttgaa
      841 gcagtagaaa cacttatttc tgacggcttt actccgaagc agacaattta tctctcgttc
      901 ggacatgatg aggaaatagg cggtaatgaa ggtgcggcaa aaaccgtcga gcttctgaaa
      961 tcaagagggg taaagctgga ttatgtattg gatgagggag gcattatcac aaatggaatg
     1021 tttccttgaa ttgatgatcc ggtggcagtt atcggcatag cagagaaagg ctatattagt
     1081 attgagctca tagtcgaggg caagggcggc cattcatcca tgcccccaaa acaaaccaca
     1141 gtcggcatac ttagcaacgc cataactaaa ctggaaagta acccctttaa gcccagacta
     1201 gacggcgccg caaagaaaat gtttaaaaaa ttaacacccc atatgccttt ttatgaaagg
     1261 atggggctgg caaatttgtg gatctttgag tgttttgctc ttaaccgtat aacaaaatca
     1321 cagagcgcaa acgccatggt aaggaccacc actgctccca caatgctgca aggcagtaac
     1381 aaggaaaatg tgcttgccgc aaaggcaaag gcggttgtga atttcagggt ccttcccggt
     1441 gacactactg aaacagtaat aaaaagggtt acaaaagtga tagacgataa aagggtaata
     1501 attaaccctg gtactttaca aattaacccc tcacctatat cagatattaa tacatcattt
     1561 tataaagtgc tagaaaaatc tatccgtcag catacagaag gaaacgtaat cacagcacct
     1621 tatcttgtag taggtgcaac agactcaagg cattttatcc cgcttacaaa aaacatatat
     1681 aagtttattg gaattactct ttcttcctcc gacctcaaag gatttcacgg cacaaatgaa
     1741 aaactatctg cagacgaata tataagggca gtaaaaatct attatcagat tattaaaaac
     1801 atcaacaaat aaaacactac ttttctgttt caactgtaac atcggtaaac caaagcctga
     1861 agtcatttgc accaacatgg ttttctgaaa ttgttattcc gctgtagctt ttgttctgct
     1921 cccatgtcat ggcttttttg ttatccaccg agccgccttt tctaaatacc cattccttaa
     1981 ttatgaagtc gtcatcataa tacagctcat atatatcatt tggagtgtaa ccggcgtcgt
     2041 ttacatattt aacttccaat ttgttaagac tttcattatt tattggagag ctctgcttgc
     2101 cgtctgcttc agtaaactct acgttttcat cccagagaag atggaacgga aaaagcagcc
     2161 agtatttatc gtttataaac ttgtgatctg tatcttcaag atcgtctgtc acattgtttg
     2221 ttctatattt aatattctca ttaacatata tcacatgacc agactttgga taccatatcc
     2281 actttctctg tatgtccttg tcgttgatgt gaacattaaa agtgtatgag atttttatta
     2341 ctttattaaa ctcttcgatc ccgtaagcgt ttgctatatt ctctgcggat ttgggtattg
     2401 aataagcaat tgatgtaccg aataagaata atagtgctaa ttgtaaccaa acgtattttt
     2461 ttatcatttt aaaactcctt gcagccagtt agaaataaga gtaatatcca attttttaca
     2521 catcctgtca aacctcgaat tcacagttgt gttgtatagt aaaagatgca taaactaaat
     2581 tagtaataga ataatttttt cttgtctcta atctcatatg aattaataat tttatactag
     2641 tgagaattta cagaaactaa aatattttat aaatttctaa aaaacctctt gacatgaaat
     2701 aacaaatgct ttattattgt actgactggt caactagaca cttaagataa attggccatc
     2761 agataaaaac aggaaataaa aacagaagaa acaaaatgac gacaaaagaa aaattaatag
     2821 acagcgcaat tcagttactt ggaaccagaa gctataactc tgttggtgta caggagctgt
     2881 gtgagcatgc agaggttaaa aaaggaagtt tctatcattt tttcccatca aagcgtgacc
     2941 tcacaattgt agcgctggat actatttggg aaaactttaa aacccacatg ttacttccgg
     3001 tactcgaaag tgacctagaa ccactggaga aaatgaaaac ccttattgat aatggatata
     3061 agcaccttgt ggattcaaag tcttgcactg gctgtatttc aggttgcaat ataggaaacc
     3121 tggctgttga actcagcact caggatgaag aaattagatt gaaaatagca tcaatatttg
     3181 atgaatggac aaggttaata gaagtactta tacaggaggc aatatccaaa ggacagataa
     3241 gttgtaaagt aaatgtcagg gcttcggctc agtctctact ggcttttgta gaaggtgcac
     3301 tgcttgtgtg taaaacatac aacgatccag agattatgaa aaccatgtgc gacaatgcat
     3361 ttaatatgtt aatggtaaga aaataaaaaa atttacgcaa atatttactg actggtcaac
     3421 tagccggtaa ggagtgtagc aaatgttaag acaaccaatt ttcatagata acaaggtgat
     3481 tccaatacta agcggcaata ttgaatattt tggtgaagaa cttaattata aagaccttaa
     3541 ggacgaacag cttgcatctt tgtatgttaa aaatagagat gacaatgcgt ataatgaaat
     3601 tgtaaacaga tatggtgaga agattttcag gcttgcaatg agaattacta aaaaccccga
     3661 aagtgccgaa gaagtacttc aaaatgtgtt tgttaagcta atagaaaagc ttggcacatt
     3721 cagagaagaa tcaaagcttg ctacatggat ttacacagta agctcaaacg aagcgttcat
     3781 gtaccttaga ggaaagaaca aaaacagctc aaaggaaata agcgtagaag agtttaataa
     3841 taatgacagc ggctcatact atgaaggttt gcaattaaaa tatgatggtt ttggcccgga
     3901 tgaatcggta aataacatgg agcagcaaga aattttagat aaagcaatag gtgaactgcc
     3961 cgaagaatat agagtagtat ttcagctaag agatgttgaa ggcctgtcta accagcagac
     4021 tgcagatatt ctaggattat ctcttccagc tgtaaaatca aggatattaa gggcgaggaa
     4081 tcagcttaaa aagaggctag ccgaatattt ccctgagtat aaggatagtt agataatatt
     4141 ataaaacaga ttgtttctta ctcagcagca gattttacta ctgtttccag ctcgccattt
     4201 tgaaacatct ctgtgcagat atcacagccg ccaataaatt caccgtttac atagagctga
     4261 gggattgttg gccagttgga aaaatctttt attccctgcc tgatttcagg atcgagcaaa
     4321 acatcgacag tctcataagg aacgtcatat gaatttaata tttgtaccac ctgtgcagag
     4381 aagccgcact gaggggcttg tttgttgcct ttcataaaaa gaatgacttt attatcatct
     4441 atctgctttt taatagattg ttgaatatct gacatattat tactcctatc ttaaatttaa
     4501 tttctcatat cctgccactg ctgaggggta taggttttca tcgaaagagc atggatatcc
     4561 tctttcattg catcaccaag agctctataa actacctgat gtctttgtat tagtgtttta
     4621 ccctcaaata ggtcagagac aattaccgct tcaaaatgtg ctccgtcacc ttctactgca
     4681 acatgctctg cattcatgcc gttttcaata agttctttta ctttttcagg atccatgaaa
     4741 gcaattataa attgttttta ctccttttca atctgatgac aatgttcttt tataacttcc
     4801 agtgtaatca tcgattctct tattagctca attgccgcaa aaaccaggca tacaatgcca
     4861 agacagctaa gacccattac aaaataaaat aaatctccca accatataac tgcaatacca
     4921 cctaccaggc ttgctattgc tagaaatgta atgcttaaat acaggcttac aagtgcattt
     4981 ctaaaaataa cggctcgttt atccagattc atggccagtt tataaatatc atgatcatgt
     5041 ttatgacttg caagatcatg tatttcagaa tgaacctgtg caaatctgct tgacgtagac
     5101 attacaagca gtgccacacc cggaacaaga agtagtggag ttaaccataa ttctgcgcta
     5161 tccataacat aattagacta tcaaaaatta atagtagatt ccaataaaat ttgatatata
     5221 attaacagtg gtgttgggag gggttttgaa atgaattaaa cacagtgcta aacctttctt
     5281 gactaatata tatttaaagg gaaaatagaa aaaattttta aatttgagga gatttttaaa
     5341 atgaacaaac taattttagc acttttatta ttaccagttt tattctctgc atgctctaag
     5401 gaagaagagc ctaaatacac agaaaaagac ctacagagcc aacatcattt tgctaaaaaa
     5461 gaagctgcaa aaaaagcatt agtaaaaaga ccaaaatcga caagaaatcc tgttgcagtg
     5521 gttgacggca tagagattta tgctgaagac ctggaaaatg gaaatattga tctgactgta
     5581 aaaaatgagg ttctctatca ggttggagtg gaaaaagggt ttgataaggt tgtagaacca
     5641 aaagttacca aatatgaaag aaaccttgtt cttaaaatga tcaaggacga gcttagaaga
     5701 ggcattgaac caaaagttac cgatcaggaa actgttgatt actacaacaa tcacaagcat
     5761 gaatatactc ttgttgaagt agaagaactt ataacggcaa ccagagactt ggccgaagag
     5821 gtaagaaaag aagctcttaa aggtacagat tttaaacaga tcgttcagtt aaacacgaca
     5881 gaacaaggag caacagtagc attcaaccag acttctaatg tagtaagagt gtttaatgaa
     5941 gcagttgatc ttggtcaggt aagtcaggtt attaactcag gcagtcagtt catagtgcaa
     6001 aaggttataa ataagtcaga gcccggtttt gatgacttaa aagacaccat cgcaattttt
     6061 cttataaaag aaaaagccga taatgaatat gaatcaataa ttgaaaagct aaaagaagaa
     6121 aagaatgtag agattttggc tgaagctgaa aaataggctt cagaataatt tctgtatttt
     6181 ttataaatat acttttatag ctgtattcat cttacacttt tcattctatt tacatactgc
     6241 ggatttatta tttgttaaac taagcagttg aaaaccgtcc atttacctga tagcttgatc
     6301 gtataatggc taagaaaatc aatgtagcaa tattgggagc aagcggatat acaggtggtg
     6361 aactgctcag gtttttagta aatcatcccc ctgtcgagat aactcatctc acagctgaca
     6421 ggagtgtagg aaaaaatctt tcagatgtgc tccctacttt tagggacatt ttagacataa
     6481 aacttcatcc tctgcagctt gatagaattc ctaaaaatat agattttgct tttacggctt
     6541 taccgcacgg aacttcggga aacgttgtaa agcatctata tgaaaaagga ataaaagttg
     6601 tcgaccttgg ggcggatttc agaattagcc ataatgttta taaaaaatgg tacggtgagc
     6661 acccttgccc tgagcttatt gataaagcag tgtatggtct accggagctt catagggcaa
     6721 agatcaagag agctaaaata atagcaaacc cgggatgtta ccctacatca tccacccttc
     6781 ctctagctcc tcttcttaag aacaatttaa taaaacagaa cactattatc attgattcca
     6841 aatcaggggt ttcaggagcc ggtcgttcag ctgctcttga ttataatttt tgtgaagtaa
     6901 acgaaggtct taaagcatac aagatcggag agcacaggca tatgcctgaa attactgaag
     6961 tgctatcaaa gtattctcat aaaagagtct tattaaattt cactccacac cttatcccta
     7021 tggacagggg aatactgtct acaatatatg tagaccttaa taagaaaatg gaaacaactg
     7081 acctgcttga tgtttataag aaattttacc gcatagaaaa gtttgtaagg ataatgccag
     7141 aaggcgtcta tccttccaca catcaggtgc gcggttctaa tttctgtgat atagggtgta
     7201 aggttatccc tgctcagaat aaggcaatct tagtttcagt tatcgataac cttgtaaaag
     7261 gcgcttctgg ccaggcagtg cagaatatga atctgatgat gggataccct gaagatacag
     7321 gaattaacct agtaccggtt tttccttaag ttttgttaat atatcttatg cgttctacaa
     7381 ttgcgggaag tttatctaat acaagatcaa tatcgctctc ttttgaaaac ctgccgaagc
     7441 tgaaacgtag ggatgaaatg gcttgttctt ttgtaagccc cattgcaagg agcacatgag
     7501 atgggtctac atttccctca gagcatgctg aacctgtgga aaccgctact ccttcaatgt
     7561 cgagattcat aaccagtgat tcaccttctg tgttatcaaa gtttaggtta agagtattcg
     7621 aaagtctgtt ttctaagtgg ccgtttaagc tcactccatc gatactattt gttatttttt
     7681 tatgtagttt atccctaagt gcagaaagtc tgttattttc tgcttctata tcatctataa
     7741 taagctctgc agcttttcca aatccggcaa ttgcgggaac attctctgtc ccggagcgct
     7801 tacctctttc ctgaccaccg ccgtgtatga gtggactcag atctacccct tttctcatgt
     7861 acaatgctcc ggctccctta ggtccgtaaa gcttatgtga ggataaagat aacaaatcaa
     7921 ctggtaattc tttcagattt atctctatct ttcccactgc ctgtacagca tctgtgtgaa
     7981 gtaaaactcc ctgttctttt gtaatctctg aaatctgatc tagtggaaag atcactccgg
     8041 tttcattatt tgcatatat
//
LOCUS       DBNGSV010000368         4482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1866777, whole genome shotgun sequence.
ACCESSION   DBNGSV010000368 DBNGSV010000000
VERSION     DBNGSV010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1866777"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /gene="pdxH"
                     /locus_tag="AC159U_13105"
     CDS             complement(<1..585)
                     /gene="pdxH"
                     /locus_tag="AC159U_13105"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3338034.1"
                     /translation="MSVKDFNKQYIYSELLESQIDPDPIKQFEMWFKESADKGIKYPN
                     AFALSTSTLDGKPNSRFVLLKGFDKSGFVFYTNTESVKGEEINDNPNASMVFWWDMVE
                     RQVRINGTINLVSDEEADEYFKSRPRGSQLGAWASKQSTVIENRDSIDQSYAQVEKKY
                     EGKDIPRPPYWHGYRLRPDSIEFWQGRPDRLQDRL"
     gene            complement(612..1502)
                     /locus_tag="AC159U_13110"
     CDS             complement(612..1502)
                     /locus_tag="AC159U_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3338035.1"
                     /translation="MNIAITGATGLIGQNLSQFLERNGHKVSKLSRSKTNVKDFVHFD
                     YKSKEIELDKLEHVDVIVHLAGESIAGYWTKHKKEEIKTSRVEGTRFLVNSILKLQNK
                     PKHFICASAIGAYGNRDDEIVDEKSDYGNGFLADISKDWENEASVLESHNIRVVNLRI
                     GLVLSNLGGALKSMLLPFKMGVGGKLGSGQQYMSWVMLEDVVGAIDFIINNKDINGPV
                     NIVAPKPVTNEQFTKELGKALGRPTILNVPAFVLNTLVPDMAKEIILSSTRAVPQKLL
                     DKGYQFQYTDIDSAFDYLLN"
     gene            complement(1535..2671)
                     /locus_tag="AC159U_13115"
     CDS             complement(1535..2671)
                     /locus_tag="AC159U_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3338036.1"
                     /translation="MCLNNRESSNLLDKSKMHSENILNFEKTELQSIENVIMQTHEDK
                     ITTKQDIKAVKKNKQSKKSIIQNTSYQNLYFDELSNLTLLNTEQEKELSSNIIECKNK
                     ILELNKAKNNIEQSREQNKKELKRIAIALQKYQIKELEYKQKFISSNLRLVVSFAKRY
                     TGNGMSLLDMIQEGNLGLIRAVEKYDYRKGVKFSTYAAWWIIQNITRALIEKSKLIKI
                     PHYLIEKRAKIFKLRDRILEETGTEPMVEDIAEQLEINPDGVREILYSNSTVSSLDAP
                     VSLNESITHKDFLEDKGAISQEDVVNSMENIKILSKCIKLLDERERNVLTMRFGLKNS
                     KVLTLDEIGKRYSLTRERIRQIEKTAISKIKNCSDGKIYFVQNN"
     gene            2840..3781
                     /locus_tag="AC159U_13120"
     CDS             2840..3781
                     /locus_tag="AC159U_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3338037.1"
                     /translation="MTEKMFGISIVSKKTGLSAHAIRAWQKRYSAVTPIRTQTNRRLY
                     TQSHIARLANLKTLCDSGHTISNIANLTDLELMELIEQSPKSESRSKDIIYTNNEATN
                     YVKDSIDSILNYNSSKLQQIFTKAHIELGNIGFINKLLVPLMEQIGELWREGNLRIAH
                     EHLTSNLIRTFIGNLINSSTAYGSSQTIVVTTPFGQIHELGSILVACTAATEGWNVKY
                     LGANLPSEEIAAAAVANNANVVALSVVYPNFDSSVLEELKRLTTLLPDNITIFIGGRA
                     APNYKPFLLNENIRFIDSLMELRKNLESLICIDIATN"
     gene            3883..>4482
                     /locus_tag="AC159U_13125"
     CDS             3883..>4482
                     /locus_tag="AC159U_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR3338038.1"
                     /translation="MGNQDIAISHGLTEQEYKKIVKFIGREPNYTEIGIMSAMWSEHC
                     SYKSSRIHLKRLPTTGDKVIQGPGENAGIVDIGDGLCAVFKMESHNHPSFIEPYQGAA
                     TGVGGILRDIFTMGARPIALLDSLRFGNPRLSKTKYLVEGVVSGIAGYGNCIGIPTIG
                     GEIYFNSCYDGNPLVNVFALGITKTTEIFRGYATGVGNPV"
BASE COUNT         1403 a          849 c          702 g         1528 t
ORIGIN      
        1 aagtctgtct tgaagtctgt cgggcctgcc ctgccagaac tcaattgagt cgggcctgag
       61 cctgtatcca tgccaatacg ggggtctggg aatatcttta ccttcgtatt tcttctctac
      121 ttgagcataa gattgatcta tagaatccct attctctatt acagtgcttt gtttagaagc
      181 ccaggcacca agctgactgc ctctgggtct tgatttgaaa tattcatcag cctcttcatc
      241 gcttacaaga ttaatagttc catttattcg tacctgtctt tccaccatat cccaccaaaa
      301 taccatagat gcgtttgggt tatcatttat ttcttctccc ttaacactct cagtattggt
      361 ataaaagaca aaaccacttt tatcaaaccc tttaaggagc acgaatcttg agttaggctt
      421 gccatcaagc gtagaggtag agagggcaaa agcattagga tattttatac ctttatcagc
      481 tgattcttta aaccacatct caaactgctt tataggatcc ggatctattt gagattctaa
      541 caattcggaa tatatatatt gtttattgaa atcttttaca ctcatcagca gatcaatata
      601 gattgaaata tctaatttaa caaataatca aatgcactat caatatctgt atattgaaat
      661 tgataacctt tatctagcag tttttgaggc actgcccttg tactgctaag gattatttct
      721 tttgccatat cgggaaccag ggtatttaga acaaatgcgg gaacgttaag tattgttggc
      781 cttccgaggg cctttcccaa ctctttagta aactgctcat tagtaaccgg tttaggagct
      841 acaatattta caggaccatt aatatcctta ttgttgataa taaaatcaat agcgcctaca
      901 acgtcttcaa gcataaccca gctcatgtac tgctgaccgg aaccaagttt accacccaca
      961 cccattttaa aaggtaaaag catagacttg agtgctccgc ccagattaga caacaccaga
     1021 cctatacgta aatttaccac acgtatgttg tgactttcta aaaccgaggc ttcattttcc
     1081 caatcttttg atatatcagc caagaatcca ttaccgtaat cacttttctc atcaactatc
     1141 tcatcatcac ggttaccata tgcgccaatt gcagaagcac agataaaatg ctttggttta
     1201 ttttgcagtt tcaaaatact attaactaaa aaccttgtgc cttctaccct gcttgtttta
     1261 atttcttctt ttttatgttt agtccaatac ccagcaatac tctccccagc taaatgaaca
     1321 ataacatcta catgctcaag tttatccaat tcaatttctt tggatttgta atcaaaatga
     1381 acaaagtcct taacattagt ttttgatcta ctgagcttag agaccttgtg tccatttcgt
     1441 tcaagaaatt ggcttaagtt ttgcccaata agtccggttg caccggtgat tgcaatattc
     1501 atatcaaata atctacatta aaaatatcaa ttacttaatt attctgcaca aaataaattt
     1561 taccatcaga acaatttttt attttagaga tagctgtttt ttcaatctgt ctaattctct
     1621 cacgtgtaag gctatacctt ttcccgatct catcgagcgt aagtactttt gagtttttaa
     1681 gaccaaatct catcgttaaa acatttcttt ctctttcatc cagaagcttg atacatttgc
     1741 tgagaatctt tatgttctcc atgctgttaa ccacatcttc ctgactaatt gctcctttat
     1801 cctctaaaaa atctttgtgt gtaatcgatt catttagaga cactggggcg tcaagcgaac
     1861 taacagtaga attagagtat aatatttccc ttactccatc agggttaatt tcaagctgct
     1921 cggcaatgtc ttctaccata ggctcggttc cggtttcttc aagtatccta tctctaagtt
     1981 taaatatttt agcccttttt tcaataagat aatgtggaat tttaatcaac tttgattttt
     2041 caattaaggc tcttgtaata ttttgtatta tccaccaagc cgcataggta gaaaatttaa
     2101 caccttttct ataatcatat ttctctacag cccttattaa accaagattt ccttcctgaa
     2161 tcatatctaa caaagacatt ccatttcctg tatatctctt tgcaaaagaa acaacaagtc
     2221 ttagattaga actaataaac ttttgcttgt actcaagctc ttttatctga tacttttgca
     2281 gtgcaatagc tattcttttt aattctttct tattttgctc cctggattgc tctatattat
     2341 tttttgcctt gtttaattct aaaatcttgt ttttacactc tataatattt gaagatagtt
     2401 ctttttcctg ctcagtattt aataaagtta gattagaaag ttcatcaaaa tataagttct
     2461 gatagctagt attctgaatt attgattttt ttgattgttt atttttttta acagctttta
     2521 tatcttgttt agttgttatt ttatcttcat gcgtttgcat gatgacgttt tcgatagatt
     2581 gcaattccgt tttctcaaaa tttaatatat tttcactgtg cattttactt ttatctaata
     2641 gatttgaaga ttccctatta tttagacaca ttgttttcca cctcgcttgt agatatattg
     2701 tctatattat taagcaatgt ctagcatatg tcaataataa ttttgattat ttgattaatt
     2761 gcagtgttat aatctaaaca atgatcgatt tatcttatat atgcttgaca aatattagac
     2821 aacttgatac gattcaacta tgacagagaa aatgtttggg ataagtattg tatctaaaaa
     2881 gaccggacta tcagctcacg cgattagggc atggcaaaag agatattcag cagttacacc
     2941 aattagaaca caaactaaca ggcggctata tacacaaagc catattgcaa gattagctaa
     3001 tctaaaaact ctttgtgatt caggtcatac tataagcaac atagctaatt taacggattt
     3061 agagctaatg gaactaatag aacaatctcc aaaaagcgaa tccagatcaa aagatattat
     3121 ctacacaaat aatgaagcta ctaactatgt taaagacagt atcgatagca tactaaatta
     3181 taattcttca aaactacagc aaatattcac taaagctcat attgaattag gaaatattgg
     3241 gtttataaat aaattacttg taccactcat ggaacaaatt ggtgagcttt ggcgcgaagg
     3301 caacctaaga atagcacacg aacaccttac atccaattta ataagaacct tcattggaaa
     3361 tctcattaac agctctactg cttatggcag ctcacagacc atagtagtca ctaccccgtt
     3421 tggacagata catgaactgg gatcaatact ggttgcctgc accgcagcca ctgaaggatg
     3481 gaatgtaaag tatctgggcg ccaaccttcc aagtgaagaa atagcagctg cagcagtagc
     3541 taataatgct aatgttgttg ctcttagcgt tgtatatccc aattttgatt caagtgtatt
     3601 ggaagagctt aaaagactaa ctactcttct tccagataat atcacaatat ttatcggagg
     3661 tagagctgcg cctaattata aacctttttt attaaatgaa aatataagat ttatagactc
     3721 actaatggag ttgaggaaaa atctggaatc tttgatatgt atagatatag ctactaatta
     3781 actcagattc caattatata tattttaaat atttactttt taatataaaa catttatttt
     3841 tatccttttt ttaattcacc tcatctaaat aatatatctt ttatgggaaa tcaggatatt
     3901 gctataagcc acgggcttac tgagcaggaa tataaaaaaa ttgttaaatt cataggtaga
     3961 gaacccaatt atacagaaat tggaataatg tctgccatgt ggtctgagca ctgctcttat
     4021 aaaagctcaa ggattcatct aaaaagactg cctactacag gggataaggt aattcaggga
     4081 ccaggagaaa atgcaggcat tgtagatata ggcgatggac tatgcgcagt atttaagatg
     4141 gaaagccata atcacccttc atttattgaa ccttatcagg gagctgccac tggtgttgga
     4201 ggaatactta gagacatttt tacaatgggt gctagaccca tagcactttt agactctctt
     4261 cgattcggta atccccgact ttcaaagaca aaatatcttg ttgaaggagt tgtctcagga
     4321 attgcgggtt atggaaactg catcggaatt ccaaccattg gaggagaaat ttattttaac
     4381 agctgctatg atggaaatcc tcttgttaat gtatttgcac ttggaattac aaaaacaact
     4441 gagatattca ggggatatgc cacaggtgta ggaaatccgg tg
//
LOCUS       DBNGSV010000369         3835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_135900, whole genome shotgun sequence.
ACCESSION   DBNGSV010000369 DBNGSV010000000
VERSION     DBNGSV010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_135900"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..642
                     /locus_tag="AC159U_13130"
     CDS             274..642
                     /locus_tag="AC159U_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338039.1"
                     /translation="MVTRCLLTVLLAAGLFIFADTGTAEAGAAISGDSQVLSLSAIRN
                     NGQTPIQGAEFPPDEWKLQRLSDHAAVYTCTDRSGCSFDIIFSQFPDNSGRSARNFSR
                     NAKVSVSQHSIIIPFECEDP"
     gene            666..992
                     /locus_tag="AC159U_13135"
     CDS             666..992
                     /locus_tag="AC159U_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338040.1"
                     /translation="MIKKLILILMLSVMGYAGLSSNANAGSTIFPKVISLTSIGGAFF
                     DHSDGWTIDIVNSNSVQYECGEETCSFNVSNRNFNNSAFYGCPEGAVVAISQNAIHIP
                     KECGDD"
     gene            1010..1357
                     /locus_tag="AC159U_13140"
     CDS             1010..1357
                     /locus_tag="AC159U_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338041.1"
                     /translation="MYKKIILSLFIGFFALTFAPSLSYGGGGFSPDTPADSQALSLVD
                     IAGATFPNGQWELERINSDSAKYVCISNNNCQFDVFTLGFTTSPRYCDTGAVVYVAQN
                     ALIIPFECSTSEE"
     gene            complement(1738..2568)
                     /locus_tag="AC159U_13145"
     CDS             complement(1738..2568)
                     /locus_tag="AC159U_13145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3338042.1"
                     /translation="MTEGKQVSEHYSHGNLLEAIEDALSKLGKSPKNVTIHDLAPVDE
                     FHIGGRQATERFLKQLEFPQKGHILDVGCGLGGAARFVADMFDHLVSGIDLTKEYIDT
                     GNALCAWVGLDNHITLHEGSALSMPFESETFDGAYMMHVGMNIKDKEGLFKEVYRVLK
                     PGAVFGVYDVMRDSAGELAYPVPWATDSSTSHLGTPSEYSQALGEMGFEVLEINNRID
                     FAMEFFRQLKEKTEDAESTPPLGLHTLMQKTTAEKVKNMIDNIANGIIAPVEIVAKKV
                     "
     gene            3094..3258
                     /locus_tag="AC159U_13150"
     CDS             3094..3258
                     /locus_tag="AC159U_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338043.1"
                     /translation="MSAVLSNNLNDAQSTSLSRTGGRIADIDESSWTLMGGDTFRVGG
                     KVEKELPRLE"
     gene            3265..3732
                     /locus_tag="AC159U_13155"
     CDS             3265..3732
                     /locus_tag="AC159U_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016991.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR3338044.1"
                     /translation="MRSADYLAYYTNPHARGIIKALVQLTIGSGANLILDPSLDEKQK
                     QAHTKIWENFCMENNWRKKQLEIARRTFRDGESILRFLNESTLRFHFIEPDWIDSDNP
                     DISHSIEIETSDISRGRNYRIQNPHTDSEEIIDPAEIQHIKINVDENEKKRPL"
BASE COUNT         1159 a          795 c          806 g         1075 t
ORIGIN      
        1 agtgaaatgg gcttacagaa accatagagg actggctaaa cacaagagaa ttaattactt
       61 caaatggtac acctttttaa agcaaatctg actcaaatta gcacacattt tttgcaatta
      121 tgcccgaaat gacacaacta ttatgtcatg ctcgagtact tcggcttcgc ctcggaataa
      181 cagggcagtt tataacttaa ttatttaaat aaattaaatt aaaaaacatg gcagagtaaa
      241 attagcttat taaatcatag gagataagga gatatggtaa caagatgttt actaacagtg
      301 cttttggctg cgggactttt tattttcgct gacacaggca cggcagaagc aggggctgcg
      361 atatcgggcg attcacaggt gctttcgcta tctgctataa gaaacaatgg gcagaccccg
      421 atacagggcg cggagtttcc gcccgatgaa tggaagctgc agagactgag cgaccatgcg
      481 gctgtgtata cttgtacaga cagaagcggg tgcagctttg acattatttt cagccagttt
      541 cccgacaaca gcggcaggag cgcacgcaac ttcagcagga atgccaaggt gtccgtttca
      601 cagcactcaa taatcattcc ttttgaatgc gaggatccat aatctttaag gaggataata
      661 taacaatgat aaaaaaatta atactaatat tgatgttaag cgttatgggc tatgcaggtc
      721 ttagctcgaa tgccaatgca ggtagtacga tatttcccaa ggtgatatca ctcacatcca
      781 tcggcggagc gttttttgat catagtgatg gctggaccat agatatcgtt aactctaatt
      841 cagtgcagta tgaatgcggc gaagaaacat gtagttttaa tgtttccaac agaaacttta
      901 acaactccgc tttctatggc tgccctgagg gtgcagtggt ggcaatatct caaaacgcaa
      961 ttcacattcc aaaagagtgt ggagatgact aaaggaggaa aatataaaaa tgtataaaaa
     1021 aataatatta agcttgttta tagggttttt cgcccttact tttgctcctt ctctgtcata
     1081 cgggggcggt ggcttcagtc cagacacgcc tgctgattct caggcgctct cgcttgtaga
     1141 tattgccggg gccacttttc caaacggaca gtgggagctt gaacgcataa attcagactc
     1201 ggcaaagtat gtatgcataa gcaacaacaa ctgccagttt gatgttttta ctctcggttt
     1261 taccacaagc ccgcgctatt gtgatacagg cgcagtggtc tatgtagcac aaaacgcgct
     1321 tattattccg tttgagtgtt ctaccagtga agaataacag attggaaaat cgaaaataat
     1381 taattttaaa ccctgctctt gagatttaga tttgggagcg ggggattttt atttctgacg
     1441 tgtacctcaa ctaaaaacca ctatgtactt ttccttgatg aataaacggg ttttttcgat
     1501 tagaaaaaat tacggtcggt caggatagga atctagtacc tgatagtttg cacaagagta
     1561 gatgaaacca aaatacaaaa atcaccgact gaataagaat tgctaaagat taatcgttta
     1621 tcctaaaccg ctgtaatctt cccacacccc cgacacacaa agaagggtcg aagtgtgagg
     1681 tttgttacga tttcgactgt tctcaatgcg tttgataaat actaaccaaa gaggctgtca
     1741 caccttctta gcaacaatct caactggggc gattattcca tttgcgatat tgtctatcat
     1801 attctttacc ttctcggctg tagtcttttg cataagggta tgaagaccaa ggggcggagt
     1861 gctttcggca tcttccgttt tttcttttaa ctgacggaaa aattccatgg caaaatctat
     1921 acggttattt atctcaagca cctcaaaccc catttcaccc agagcctgtg agtactcaga
     1981 aggggtgcct aggtggcttg tggagctgtc cgtagcccac ggtacggggt aggcaagctc
     2041 tcccgcagag tcgcgcatca catcatacac cccaaacacg gccccaggct ttagcaccct
     2101 gtacacctct ttaaaaagtc cttccttatc ttttatgttc attcccacat gcatcatgta
     2161 cgcgccgtca aacgtctcgc tctcaaaggg catggagagg gcgcttccct catgaagggt
     2221 tatgtggtta tcaagcccca cccacgcgca gagcgcattt cctgtgtcta tgtattcttt
     2281 tgtaaggtct atacccgaga caagatggtc aaacatatca gccacaaacc tcgccgctcc
     2341 gccaagaccg cagcctacgt ccagtatgtg ccctttttgc ggaaattcga gctgttttaa
     2401 aaacctctca gtagcctgcc ttccgcctat atggaattca tccacgggtg caaggtcatg
     2461 tattgtcaca tttttgggtg acttaccgag cttggaaaga gcgtcctcga tggcctcgag
     2521 caggtttcca tgggaataat gttctgatac ttgtttgcct tctgtcatgc tgtggtctcc
     2581 ttaacactgc tattattttt ttatataatt ataattatac attcaattag tgcagttaat
     2641 tgcattaatt tttttgatgt aatggtcgga taaaatatgc aagattcttt cctaaaaacc
     2701 acgtttttac aaaaaaacgt aaattttttg aaatattttt gtaaaatcta caaaatttct
     2761 aaaattcgaa aaccgatgta actctctgta ataatacaag atatggtgta attagtaaaa
     2821 tcgtccacaa aatgtgcttg acaaatatgc tggatttggt aaattgatac tcacgcgaaa
     2881 ttgtatctaa agcaatctcg cataggtctt ttaattcggt gtccggtaag gtggaaatta
     2941 ccacaccgta tttaaaaccc tcacaaaaaa gctcctggca gtggaaagtc tttcaggggc
     3001 ttttttatgg ctaatttccc caaataatct caaaacctga aatcacttta acgaaaatat
     3061 aaacataata gaaaaacttt ttatggaagg actgtgtccg cggttctaag caacaattta
     3121 aatgacgctc agagtacatc cctttcccga acaggcggca ggattgccga tattgatgag
     3181 tcttcttgga ctcttatggg tggagataca ttcagggtag gcggcaaggt ggaaaaggag
     3241 cttcctaggc tcgagtaaaa ggagatcaga tccgcggatt atcttgcata ctacacaaac
     3301 ccgcatgcaa gggggataat aaaagccctt gttcagctta caataggctc tggggcgaat
     3361 ttaatattag acccgtctct tgatgaaaag cagaaacagg ctcataccaa aatatgggaa
     3421 aacttctgta tggagaacaa ctggagaaaa aaacagcttg agattgcccg ccggacattc
     3481 agggacggtg agagtattct gcgcttttta aatgaatcca ctctccgttt ccattttata
     3541 gagcctgact ggatagattc tgataatcca gacatttctc acagcattga gatagagaca
     3601 agcgatatat cgaggggcag aaactacaga atacaaaacc ctcatacaga ttctgaagag
     3661 atcatagacc ccgccgagat acagcatata aagattaatg tggatgaaaa tgaaaaaaag
     3721 aggccgctat aagcttgagc ctgttcttta tgacctaaaa gaacttcagg acttcagaaa
     3781 ataccgtgtg atgctcaaca agatatgctc ttctgtagtg atagtgcata aaaaa
//
LOCUS       DBNGSV010000370         3073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_898771, whole genome shotgun sequence.
ACCESSION   DBNGSV010000370 DBNGSV010000000
VERSION     DBNGSV010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_898771"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /gene="gatB"
                     /locus_tag="AC159U_13160"
     CDS             complement(<1..1345)
                     /gene="gatB"
                     /locus_tag="AC159U_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3338045.1"
                     /translation="MNYEAVIGLEVHAQLSTDTKIFCSCSTKFGAEPNSQVCPICLGM
                     PGVLPVLNKKALEYATMAAIATNCTVHNVSRFDRKNYFYPDLPKGYQITQYEQPLCTG
                     GQLDIDLDGETKKIRINRIHMEEDAGKLIHEDSEHYSFVDLNRTGVPLIEIVSEPDMS
                     SGDEAVAYMKKLRSILTFIGVCDGNMEEGSLRCDANISVRPVGSDKLGTKAEIKNVNS
                     FRFVQKAIEYEIERQISVLESGGQVIQETRLFDSNKNVTFSMRSKEEAHDYRYFPDPD
                     LLPVVLEDKEIETIQGSLPELPDKRYDRFIKDYKLPDASALVLTSTKALADYFEDALE
                     FCSDTKIVSNWIINEVLREIGDEEEIVNFKITPKELSKLLGLIHKGSISGKIAKDVFS
                     QMIDTGQSAEQIVKEKGLEQISDEGELESLIEAIVNKHPDEVKRFKDGDQKLLGFF"
     gene            complement(1512..>3073)
                     /locus_tag="AC159U_13165"
     CDS             complement(1512..>3073)
                     /locus_tag="AC159U_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR3338046.1"
                     /translation="LLGLAPKTAILVKEDGTEEEIPLEHVGPGNRLRVRPGEKVPVDG
                     IIIKGATSVDESMITGEPIPAEKNPGDKVIGATVNGTGSLIIEAERVGAETLLAQIVH
                     MVSEAQRSRAPIQSLADKVSGYFVPIVVLISIITFIVWSIFGPSPAMVYAIINAVAVL
                     IIACPCALGLATPMSIMVATGKGATLGVLFKNAEAIEKLREVDTIVVDKTGTLTLGKP
                     KLVSVRTANTFTDKELLKYAASIEKGSEHPLATAIVEGADERGVGVEEAGQFESITGK
                     GVKGMVSGRRVALGNQKLMDDLGINLEGLLDEAKKLRSEGQTVMFISIDNKPAGFVGV
                     ADPIKKTTPRAVSSFHDEGIRVVMLTGDEQVTAKSVADKLGIDEVISGVLPDQKAQKI
                     SELQSEGHIVAMAGDGINDAPALAKAEVGIAMGTGTDVAMESAGVTLVKGDLNGIVRA
                     IKLSRYTMRNIKENLFFAFIYNALGVPIAAGVLYPFFGILLSPIIAAAAMSFSSVSVI
                     ANALRLKKAKI"
BASE COUNT          822 a          741 c          516 g          994 t
ORIGIN      
        1 caaaaaatcc aagcagtttt tgatcaccat ctttaaaacg ttttacctca tctggatgtt
       61 tatttacaat agcttcaatg agactttcaa gttctccctc atctgatatc tgctctaacc
      121 ctttttcttt tacaatttgt tctgcagact gtcctgtatc aatcatctga gaaaatacat
      181 cttttgcaat ttttccgcta atgctccctt tatgaatgag tccaaggagt tttgatagtt
      241 ctttgggtgt aattttaaaa ttaactattt cttcctcatc acctatttca cgcagaactt
      301 cgttaataat ccagttggat acaatttttg tatctgaaca aaactcaagg gcatcttcaa
      361 aataatctgc aagcgctttg gttgaagtta acaccagtgc acttgcatcc ggaagtttat
      421 aatcttttat gaacctatcg tatcttttgt caggaagctc aggcagagaa ccttgaatag
      481 tctctatctc tttatcttct aatacaaccg gcagcagatc agggtctgga aagtacctgt
      541 aatcatgagc ctcttctttg cttctcattg aaaatgttac atttttattt gaatcaaaaa
      601 gccttgtctc ctgtattacc tgcccgcctg actctagtac ggatatttgt ctttctatct
      661 catactcaat agctttttga acaaacctga atgagtttac attctttatt tcagccttag
      721 tacccagttt atccgagcca acaggtctta ctgaaatatt ggcatcgcac ctcaggcttc
      781 cctcttccat attaccatca catacaccta taaatgttag tatggaacgg agttttttca
      841 tatatgcaac agcctcatct ccgcttgaca tatcaggctc actaactatt tctataagag
      901 gcacaccagt tctattgaga tcaacaaagc tatagtgctc agagtcttca tgtattaact
      961 tgcccgcatc ttcttccata tgaatacgat ttattctaat tttcttggtt tccccatcaa
     1021 gatcaatatc aagctgcccg cctgtgcaaa gcggctgttc atattgagta atctgataac
     1081 cctttggaag atcgggataa aagtagttct tacggtcaaa ccttgataca ttatgtactg
     1141 tgcagtttgt agctatcgct gccatcgttg catactcaag cgctttttta ttcagaactg
     1201 gcagtacccc gggcatcccg agacaaatag gacagacctg cgagtttggc tctgctccaa
     1261 atttagtaga acatgaacag aatatttttg tatccgtcga taactgagca tgtacttcaa
     1321 gaccaatcac agcctcataa ttcattttaa taaaacctct ctgttttctt ttaaagttag
     1381 taatttataa ggattttttg cagaaaatca aatgacaaat ttcttataat tctaatcgac
     1441 ttaaggtata agtggttatt ttttttattt ttaaatattc taggtctatt agatcaacaa
     1501 aagtttatta attaaatttt tgctttttta agtctcaatg cattagctat aaccgataca
     1561 gagctaaagc tcatagcagc cgcggctata atagggctta gaagaattcc aaagaaggga
     1621 taaagaacac cagctgcaat cggtactccg agtgcgttat aaataaatgc aaagaaaagg
     1681 ttctccttta tatttctcat cgtgtatctg ctgagtttga ttgcccgcac tattccatta
     1741 aggtcaccct ttacaagcgt gacccctgcg ctttccatcg ctacatctgt gcccgtgccc
     1801 atagctatac ccacctctgc ctttgcaaga gcaggagcat cgttaatccc gtcccctgcc
     1861 atagccacaa tatgaccttc actttgcaat tcacttattt tttgggcctt ctggtctggc
     1921 aaaacacccg aaattacttc gtctatgcca agtttatccg ctacagactt tgccgtaact
     1981 tgctcatcac ctgtaagcat aacgaccctt atcccctcat catgaaaact ggaaaccgcc
     2041 cggggcgtag tcttttttat aggatcagca acgcctacaa accctgcagg cttattatct
     2101 attgaaataa acataacagt ctggccttct gaccttagtt tttttgcctc atctagcaat
     2161 ccttctaagt taatacccag atcatccatt aatttttgat tccctagagc aaccctgcgg
     2221 ccagatacca taccttttac tccctttcca gtaatagatt caaactgacc ggcctcctct
     2281 acccctactc cccgctcatc cgccccctct acaattgccg tggcaagcgg gtgttcgctt
     2341 cctttttcta tagaggctgc atatttcagt agttctttgt cggtaaaggt atttgctgtc
     2401 cttacagata ccagtttagg ctttcctaag gtgagagttc cggttttatc gacaacaatc
     2461 gtatcgactt cccttagctt ttctatagcc tctgcatttt tgaataaaac acccagtgtt
     2521 gcaccttttc ccgttgcaac catgattgac attggtgttg ccagacccaa agcacacgga
     2581 caggctatta tcaagacagc aaccgcattt attattgcat agaccattgc tggggaaggg
     2641 ccaaaaatag accagacaat aaaggtaatt atagatatta gaacaactat tgggacaaaa
     2701 tacccggaca ccttatcggc aaggctctga ataggtgcac ggctcctctg ggcttctgat
     2761 accatatgga ctatctgagc aagcagggtt tccgcaccta ccctctcagc ttcaataatc
     2821 aaagaacccg tgccatttac tgtagcccct atcactttat ctccaggatt tttttctgca
     2881 ggtataggct ctcctgtaat catcgattcg tctactgagg tagcaccttt tattatgatt
     2941 ccgtctacgg gcactttctc tccgggtcgc actcttagcc tgttaccagg cccaacgtgc
     3001 tccagtggaa tctcttcttc tgttccatcc tctttgacca ggattgctgt tttaggagcc
     3061 agacccaaga gtg
//
LOCUS       DBNGSV010000371         8012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_899011, whole genome shotgun sequence.
ACCESSION   DBNGSV010000371 DBNGSV010000000
VERSION     DBNGSV010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_899011"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..454)
                     /locus_tag="AC159U_13170"
     CDS             complement(56..454)
                     /locus_tag="AC159U_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3338047.1"
                     /translation="MSNDASSTDFLEVTLPDGNIIKAEIAMDKAQGLQNRDYLCPKCG
                     MIFIFDTEGIYSFWMKDTLLDLSLIWINKKREVVHIERNAKPCKYKTDPRKECQVYMP
                     NINAKYVLEILPGASDSINIGSVLKFIPPG"
     gene            complement(534..872)
                     /locus_tag="AC159U_13175"
     CDS             complement(534..872)
                     /locus_tag="AC159U_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338048.1"
                     /translation="MKKFQILVDDEIIYDGTFSEVPDHYRENLVSCLEEWSDVLGKKS
                     INELIYSSFYWYKQQKMYCFNCCEFIEQATECADCGHQLQNRFVHDRNTDVDRILDCI
                     GMITSVEVIK"
     gene            complement(936..3458)
                     /locus_tag="AC159U_13180"
     CDS             complement(936..3458)
                     /locus_tag="AC159U_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3338049.1"
                     /translation="MNYLNRIGERLNKQIVYFIAFVCFIVWSFCFVLALKWVNQPFTG
                     FLFYNNLVVSEVVIDLGDDKTDDKPQIITDRIIAINGERVNSPGEIYQIVNRYNVGTP
                     LDYSFIRGDKIFEYTIPVSVFNIGHFISIFGIVYFSGLAFFVVGISVYFLRPRLTTSK
                     IFLLFSVCTGLWLVSYFDSQSTFIFDKLSTLGFFLAPTLAIYLATVFPKDRPLNLRHY
                     LLLTPLFIVSVILFVISISPVGSYELWLTLSKLMLGYALIGSLVVSVSNLRTYFGSAV
                     TLEKQRSQIVVIGSVIGLLIPILIMILSYAMGMKVSFGILALILTVFPISLAYSILKH
                     KLFDVNVIIRKALVYGLLTGALGGIIAILIISFNLFLAKQGGWKNPAFFIILSAFLAV
                     ALNPLQNKIQALIDLVFFRKKYDYRKTIEDISFAMTSILNPEDIYQKIVETIENTIFA
                     KDINLLFYDEQQTLYRPYSSLEDAEEEDYILSEQNILIELVRQQKSEIFLEDIYSEDK
                     YKDNCEELEQIFGKFNSSLIMPMFVKDKLIGLMTLGEKKSELTYTSEDLELLRILANQ
                     SSVAIENALAFRLVEDYASRLEDTNIELSETQAQLIQSEKMSAIGQLSAGIAHEIRNP
                     LNIIEGARYYLANLLSESEKSESVNEYLSYIKQEVERTNNLITNLLKFSKSEAPRFEL
                     IDINTTLENTMTLLKKQLSDASVNGINVFEENLPSILGDPNQLWQVFVNIIMNAIQAM
                     PEGGDLIIKTYSNGNNSICVSFKDTGVGMEKDVVKKIFDPFFTKKESGTGLGLSITYS
                     IVESHSGKIRVDSEIGVGTEFQIEIPIGKIEVNDKVTGNYTT"
     gene            complement(3669..4739)
                     /gene="aroC"
                     /locus_tag="AC159U_13185"
     CDS             complement(3669..4739)
                     /gene="aroC"
                     /locus_tag="AC159U_13185"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3338050.1"
                     /translation="MAANSFGNHFRITTWGESHGTALGVVLDGCPSGIEISESEIQFE
                     LDRRRPGQSKITTQRKEPDKVEILSGVFEGKTLGTPIAMIVRNTDAISKSYDDIKDTY
                     RPGHADFTYDEKYGFRDHRGGGRSSNRETIGRVAAAAIAKKILSLHGVKTSGYVKQIG
                     NIICDVTDLDEIENNPVRCADPAKVQPMIDLIESVRKEGDSIGGIVEVISQGVPSGLG
                     TPVFHKIEADLAAGIMSIGGIRGFEVGLGFKVAEMKGSESNDLLYQKEDGSLGFKTNN
                     AGGLLGGITNGEDLLVRIAIKPTSSISKIQNTVDKYGKPKELKVKGRHDPCLCPRAVP
                     ILEAMVNLVLADHLLISRLARI"
     gene            complement(4749..5732)
                     /locus_tag="AC159U_13190"
     CDS             complement(4749..5732)
                     /locus_tag="AC159U_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338051.1"
                     /translation="MAVLVTGATGFLGSHIARKLVQNGEEVKILVRKSSKTANIDDIE
                     AERVYGDVTDLDSLKSAIKGCDKLFHTAGFVSFRKKDHQKMQDINVGGAVNAMKAALD
                     AGVSKVVYTSSVAAVGVDPAGGEANEETKFTLESENIPYLNTKYHGEVESLKFVEQGL
                     PLVIVNPSVVVGPGDIYLSSTAFIQWYCKKKFPGYMDGTMNIVDVEDVAEGHLLAAEK
                     GKVGERYILGNTNLTINELFDMLERLTGISRPKMKIPYTFALVSGYLVERILGIGFPN
                     YSTMDVDSVKLSKYKWFVDSSKAEKELGYKKSDIEQSIKKTVDWFKENGYL"
     gene            complement(5788..7047)
                     /locus_tag="AC159U_13195"
     CDS             complement(5788..7047)
                     /locus_tag="AC159U_13195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3338052.1"
                     /translation="MKLKIEKLIYGGYGLARTDEKTVFVTGGVPGEEVKVQITEEKKS
                     YSIAEISAIIEPSEYRVEPVCEYFGKCGGCQWQHIDYGYQIKSKEAILKESLEKIAGI
                     TDLQIEPIVASPNQYKYRVRATIHVDTVKGKHVIGFKEEKSHVLVPIDHCEICSPVIS
                     QLVKKISKFLVKYQSKTLPIDMIYILDSQSIPALSLSPKKDSSHDEVIKLYYYLKERI
                     QGVNISVDNSDENTANLEVSRLEFITSASVFAQANPQINELLIKTLIDWADLNKDHNV
                     LDLYCGYGNFSLHLAKKAKFVLSIDNNKKAIKYAIKNAKKNHIRNCHFEDWNVGKYLQ
                     RHKPTEKQELIVLDPPRNGAKEIIKSIIEISPNKILYVSCNPTTLARDLKELTSAGYK
                     LRKVQPFDMFAQTFHIESLSLLQKELE"
     gene            complement(7044..8009)
                     /gene="queA"
                     /locus_tag="AC159U_13200"
     CDS             complement(7044..8009)
                     /gene="queA"
                     /locus_tag="AC159U_13200"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR3338053.1"
                     /translation="MAYYPSEQKESCKLEIVNRSENKLYHKQFSNIVDYLDCGDVLVL
                     NNTMVIPARICGASESRDDIEILLLQKKDSNIWSCLMKKPKQGQQINIDDDLSAVVRK
                     ESSGEILLEFNSEIDSYIDKKGYMPLPPYIERKPDKSDRDNYQTVYAKHKGSVAAPTA
                     GLHFTDALLQSISDKGVAIEYVTLHVGYGTFKPVKSENVEDHSMHSEYYEIGEKACNS
                     ISLAKQRGKKVVAVGTTSLRTLESSVNEDGEINPQRGTTELFIYPGYEFKVVDSLITN
                     FHMPRSTLFMLVCAFAGKELIINAYEQAKKNRYRFLSYGDAMIIL"
BASE COUNT         2463 a         1667 c         1236 g         2646 t
ORIGIN      
        1 attatttgga ataattcaac aatcatacta ccaagtcatt aatttcaatt tattattaac
       61 caggagggat aaattttagc actgaaccaa tattaatact atcactggca ccgggcaaga
      121 tttctaaaac atatttagca ttgatatttg gcatatagac ctgacactcc tttcggggat
      181 ctgttttgta tttacaaggt ttagcatttc tttcaatatg aacaacttct cgttttttat
      241 ttatccatat taaagataga tcaagcaaag tatctttcat ccaaaacgaa tagatacctt
      301 cagtatcaaa aataaatatc attccgcatt taggacagag ataatccctg ttttgcagcc
      361 cctgagcctt atccattgca atttcggctt ttattatatt tccatcagga agtgtaacct
      421 caagaaagtc agtagaactt gcatcgttgg acacacagca caacaaacat attgtaatta
      481 acaataacgt cctcagcatc atataatcta ataaacggat acaagagata ttttcattta
      541 ataacttcta cacttgttat cataccaata cagtccagaa ttctatcaac atcggtattt
      601 ctgtcatgga caaacctgtt ttgtagttgg tgaccgcaat cagcacattc agttgcctgt
      661 tcaataaatt cgcagcaatt aaaacaatac atcttctgtt gcttatacca ataaaacgag
      721 gaataaatca attcattaat agattttttg cctaaaacat ctgaccattc ctccagacag
      781 ctcaccaggt tctctctata atgatcggga acttcgctaa atgtcccgtc ataaataatt
      841 tcatcatcta ctagtatttg gaattttttc attatggact tcttaattta acgcatatcc
      901 aatcttttca aaatcataga attaaacact ttatttcagg ttgtataatt gccggtaact
      961 ttatcattca cttctatttt tccaatagga atttcaattt gaaattcagt acctactcca
     1021 atttcactat ctactcttat tttaccacta tgactttcaa caatactata ggtaatagaa
     1081 agacctagcc cggtaccgga ttcctttttc gtaaaaaatg gatcgaaaat tttctttact
     1141 acatcttttt ccatacctac accggtatct ttaaaactaa cgcaaataga attgttaccg
     1201 ttgctatagg ttttgataat cagatctcca ccctcaggca tggcctgtat ggcattcata
     1261 ataatattca caaacacctg ccataactga ttgggatcac caagtattga tggaaggttc
     1321 tcttcaaaca cattaatgcc atttacagat gcatccgaga gctgtttttt tagcaatgtc
     1381 attgtgtttt ctagcgttgt attaatatct ataagctcaa aacgaggggc ttcagatttt
     1441 gaaaatttga gtaaatttgt gattaaattg tttgttcttt caacttcctg cttaatatag
     1501 ctaagatatt cgttaactga ttcagatttt tcgctttctg ataataggtt tgcaaggtaa
     1561 taccttgcac cttcaataat attgagtgga tttcgtattt catgtgctat gcctgcagat
     1621 aactgcccta tagcagacat tttctcagac tgaatcaact gggcctgggt ctcacttagc
     1681 tcaatattcg tatcttctag tctgctggca taatcttcga caagcctaaa ggcaagagca
     1741 ttttctatag caacagagct ctgattagct aatatacgca gaagctccaa atcttcagaa
     1801 gtatacgtta gctccgattt cttttcacca agcgtcataa gcccaatgag cttatcctta
     1861 acaaacattg gcattattaa cgatgaatta aacttaccaa atatttgctc caactcctca
     1921 caattatctt tatatttatc ttccgaataa atatcttcta agaatatttc gcttttttgc
     1981 tgcctaacta attcaatcaa tatgttttgt tcactgagta tatagtcttc ttcctctgcg
     2041 tcttcaagac tagaatatgg cctgtaaagg gtttgctgct cgtcataaaa aagcaggttt
     2101 atatccttag caaaaatagt gttttcaatt gtttcaacaa ttttttgata tatgtcctca
     2161 ggattcaaaa ttgaggtcat ggcaaaacta atgtcttcaa ttgtttttcg gtaatcatat
     2221 tttttcctaa aaaatactaa atctattaat gcctgaatct tattctgtag aggatttagg
     2281 gctacggcca aaaatgcgct taatattata aaaaatgcag gatttttcca acctccctgc
     2341 ttagcaagga acagattgaa acttattatt agaattgcta ttattccacc aagagcccct
     2401 gtaagaagtc cataaacaag ggcttttcta attattacgt ttacatcaaa aagcttatgt
     2461 tttagtatgg agtaggcaag agatatagga aaaacagtta gtatcaaagc aagaatgcca
     2521 aaagatactt tcatacccat ggcataactc aaaatcataa ttaaaattgg gataagcaaa
     2581 cctataacag aaccgatcac gacaatctgt gatctttgtt tttccagagt aactgcagag
     2641 ccgaaataag ttcttagatt tgaaaccgat accacaagag aaccgattag agcataaccc
     2701 agcattagtt tgctgagtgt tagccataat tcatagctgc ccaccggtga aatacttatg
     2761 acaaacaata taacagacac aataaaaagc ggggtcaaca agagataatg gcgaagatta
     2821 agtggcctgt cctttgggaa aaccgtagca agataaattg caagagttgg cgccaaaaag
     2881 aatcccaaag tagaaagttt atcaaaaata aaagtggatt gtgagtcaaa ataacttaca
     2941 agccaaagac cggtacagac tgagaacaac aagaatattt tacttgttgt aagtctgggt
     3001 cttaaaaaat ataccgaaat accaactacg aaaaaagcta atcctgaaaa atatacaata
     3061 ccaaaaatag atataaaatg cccgatatta aagacggaaa ccgggattgt atactcgaat
     3121 attttatctc cacgaatgaa actgtaatca aggggtgtgc caacattata tcgattaaca
     3181 atttggtaaa tttcaccggg tgaatttacc ctttcaccgt taattgctat tatcctatct
     3241 gtaataatct gcggtttgtc atcagtttta tcatctccga ggtcaattac tacctcagaa
     3301 actacaaggt tattatagaa taagaagcct gtaaaaggct ggtttaccca ttttagagct
     3361 aaaacaaaac aaaaagacca tacgatgaaa catacaaatg caataaaata tacaatttgc
     3421 ttgtttagtc tctctcctat tctatttaag taattcacaa tcctgcaaat ctcccctaca
     3481 cttcttaata gaactaaact attattatat tacaaaatta tgtattttat aagtcttagt
     3541 taaacaactt attctataaa tttatggaac ttacttaaaa aagttaaacc ttaaggtaaa
     3601 aagagtatta gacagagctg actcagcaaa aacaaatata ttaatattta gcagtatttt
     3661 aagaatttct agattctggc aagtctggaa atcaatagat gatcagccaa aaccagatta
     3721 accatagctt ccaatatagg aacagctctt ggacaaaggc acggatcatg cctgcctttc
     3781 acttttagct ctttaggttt gccgtacttg tccacagtat tttgaatctt tgaaattgat
     3841 gaagttggtt taatagcaat gcgcacaagt agatcttcac catttgtaat tcccccaagc
     3901 aggcctccgg cattgttggt tttaaaaccc agcgagccat cttctttttg atataataaa
     3961 tcattggact cagaaccttt catctctgcc actttaaagc caagccccac ctcaaaacct
     4021 ctgatcccac cgatgctcat aataccggct gcaagatctg cctcaatttt atggaataca
     4081 ggtgtaccca gaccactggg cactccctgg gatataacct caactatccc gcctattgag
     4141 tcaccctctt ttctaaccga ttctatcaaa tctatcatgg gctggacttt tgcagggtct
     4201 gcacatctga ccggattgtt ttctatctca tcaagatctg taacatcaca gataatattg
     4261 ccaatttgct ttacataacc cgatgttttt accccgtgaa gcgacaatat tttcttggca
     4321 attgcagctg ctgcaactct tcctatagtt tcacgattgg atgacctgcc gccgcctctg
     4381 tgatctctaa aaccatactt ctcatcatag gtaaaatcag catgaccagg gcggtatgta
     4441 tcttttatat catcatagga cttggaaatt gcatccgtat ttcttactat cattgcaatt
     4501 ggggttccaa gtgttttgcc ttcaaacaca ccggagagaa tttctacttt atcaggttct
     4561 ttcctttgag ttgtgatctt actctgaccg ggccttcttc tgtccagttc aaactgtatt
     4621 tctgactccg agatttcaat tccagaagga caaccgtcca gtactacacc aagggcagta
     4681 ccatgcgatt cgccccacgt agttattcta aaatgatttc caaaactgtt tgcagccata
     4741 gttagtattt agaggtaacc gttttcttta aaccagtcaa cggtcttttt tattgattgt
     4801 tctatatcag attttttata acctagctct ttttcagcct ttgagctgtc tacaaaccac
     4861 ttatatttcg atagtttcac tgaatccaca tccatagtag aataattagg aaatccaatc
     4921 cccagaattc tttctacaag atatccggat acgagagcaa aagtatacgg tattttcatt
     4981 ttaggtctcg atatgccggt aagcctctca agcatatcaa aaagttcatt aattgtaaga
     5041 ttggtatttc caagtatata acgctctccg acctttcctt tttctgctgc gagcaaatga
     5101 ccctcagcca cgtcttctac atcaactata ttcatcgtcc cgtccatata tcccggaaac
     5161 ttctttttgc agtaccactg aataaaagct gtgctagaga gataaatatc ccccgggcca
     5221 actacaacag atgggttaac aataacaagc ggaaggccct gctcaacaaa ttttagtgat
     5281 tcgacctctc catgatattt agtatttaaa taaggaatat tttctgattc aagggtgaat
     5341 ttagtttctt cattagcttc acctccagcc gggtctactc ctactgctgc aacactactc
     5401 gtatatacta ctttactaac cccggcatca agcgctgcct tcatcgcatt tacggcacca
     5461 cctacgttaa tatcctgcat tttctgatga tccttttttc taaaggaaac aaatccggcg
     5521 gtatggaaaa gtttgtcaca acccttaata gcgctcttta gagaatcaag atctgtcaca
     5581 tctccatata cacgctcggc ttctatgtcg tcaatattgg cagtcttgct ggattttcga
     5641 accagtattt ttacttcttc accattttgt acgagttttc ttgcaatatg agatcccaaa
     5701 aaccctgttg cacctgtaac gagtactgcc ataacttcct ccaattaaat caaataaata
     5761 tttttggatt atatcagtat gaccgcttta ttccaactct ttttgtagaa gtgaaagtga
     5821 ttctatatga aatgtttgag caaacatatc aaaaggctga acctttctta atttatatcc
     5881 ggcactggtt aattctttta ggtctctggc aagagtagta ggattgcagg atacatacaa
     5941 tattttattg ggacttatct caatgataga cttaattatt tccttagccc cgttccttgg
     6001 tggatcaaga acaattaatt cctgtttttc ggtaggttta tgtctttgaa gatactttcc
     6061 tacattccag tcttcaaaat gacagtttct aatatggttt ttctttgcat ttttgatagc
     6121 atacttaata gcctttttat tattatcaat gctaagtaca aactttgctt tttttgcaag
     6181 atgaagtgaa aagtttccat aaccgcagta cagatccaac acattgtgat ctttattcaa
     6241 atctgcccaa tctattaaag tcttaattag aagctcattt atctgtggat ttgcctgtgc
     6301 aaagaccgat gcacttgtta tgaattctag tctcgaaacc tcaagattag cagtattttc
     6361 atcactgtta tcaaccgaaa tatttactcc ctggattcgc tccttaagat agtaatacag
     6421 tttaattacc tcatcatgcg aactgtcctt tttgggtgaa agcgataaag caggtataga
     6481 ttgggaatct agaatatata tcatatcaat aggcagagtt tttgactgat attttacaag
     6541 gaactttgat attttcttaa caagctgaga gataacaggt gaacatatct cacaatgatc
     6601 aattggaaca agtacatgtg atttttcctc tttgaatccg atcacatgtt ttccctttac
     6661 agtatcaacg tgtatcgtcg ccctgaccct atacttatac tgattgggcg aagctacgat
     6721 aggttctatc tgtaaatccg taataccggc aatcttttct aatgattctt ttaaaatagc
     6781 ctctttggac tttatctgat atccatagtc aatatgctgc cactgacatc caccacactt
     6841 accgaaatat tcacatactg gctctaccct atactcagaa ggctcgataa ttgcagagat
     6901 ctctgcaatg ctgtagctct ttttctcctc agttatttgg actttgactt cttcaccagg
     6961 tactccgcct gttacaaaga cagtcttttc atcagtacgt gctaacccgt acccgccata
     7021 tatcagtttt tctattttta gtttcataat attatcattg catccccata acttaaaaat
     7081 ctgtatctgt tttttttcgc ttgttcgtat gcattgataa taagctcttt tccagcaaaa
     7141 gcacatacca gcataaaaag tgttgaacgg ggcatatgga aattagtaat caatgaatct
     7201 acgaccttga attcatagcc cggataaatg aataactcgg ttgtgcctct ttgaggatta
     7261 atctctccat cctcatttac gcttgattca agcgtcctta aagaagtagt accaaccgct
     7321 acaacttttt tgcccctttg ttttgcaaga cttatcgaat tgcaggcttt ctctcctatt
     7381 tcatagtatt cactatgcat actatgatct tctacatttt ccgatttaac aggtttaaaa
     7441 gtgccgtagc caacatgcag tgttacatac tctatagcta ctcccttatc acttatactt
     7501 tgcaacagtg catctgtaaa gtgcagacca gcggtaggcg ccgctaccga ccctttatgt
     7561 ttagcataca cggtctgata attatctctg tctgacttat ctggttttct ttcaatataa
     7621 ggaggaagcg gcatataccc cttcttatca atatagctgt ctatttctga attaaattcc
     7681 agcaatattt ctccagaact ttctttacgg actacagcac ttagatcatc atcaatatta
     7741 atctgctgcc cctgctttgg ttttttcata agacaggacc aaatattact gtccttcttt
     7801 tgtaaaagca gaatttctat atcatctcta ctctcagatg caccgcagat ccttgcagga
     7861 ataaccattg tgttatttaa gacaagcaca tcgccacaat caagataatc aacaatatta
     7921 gagaactgct tatgatatag cttattttca gacctgttta ctatctctaa tttgcatgat
     7981 tctttttgtt cagacggata ataggcaata ta
//
LOCUS       DBNGSV010000372        18286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1451978, whole genome shotgun sequence.
ACCESSION   DBNGSV010000372 DBNGSV010000000
VERSION     DBNGSV010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18286
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1451978"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1342
                     /gene="trmFO"
                     /locus_tag="AC159U_13205"
     CDS             23..1342
                     /gene="trmFO"
                     /locus_tag="AC159U_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR3338054.1"
                     /translation="MNANNKIVVVGAGLAGTEAAYQIAKRNIEVDLYEMRPHKKSPAH
                     NTDKFAELVCSNSLKSNYLENASGILKEEMRRLDSLIIKSADKTSVPAGKALAVDRTR
                     FADHITSVLEQNPYINIKRREFDKIPVTIGTPVIIASGPLTSKDLSNELSKITCTDYL
                     YFFDAISPIIEAESIDFDKAFFASRYDKGEDEQGDYLNCPLDRDEYYRLIDDIISAEN
                     IEVKEFDDVKYFESCLPVEVIAGRGKDSLRFGPMKAKGLTDPKTSKRPYAVLQLRKEN
                     YAGSMYNMVGFQTRLKYSEQKRVFSNISGLENAEFLRYGSMHRNTYINSPKLLQPTLQ
                     LKNHPNIFLAGQIVGVEGYVESAAMGLIAGINAVKLTQGCEPKIPCEQTSIGSLLKYI
                     TDSGLKEFQPMNINFGLYPEVEKKYTKTEKRRYIAERALSKITEYIN"
     gene            1353..2243
                     /gene="glyQ"
                     /locus_tag="AC159U_13210"
     CDS             1353..2243
                     /gene="glyQ"
                     /locus_tag="AC159U_13210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3338055.1"
                     /translation="MTFQKLLLELQNYWSKKNCIIYQPYDLEKGAGTFHPATFLRCLG
                     PEPWQVAYVEPSRRPTDGRYGDNPNRLQHYYQFQVIIKPSPDNIQDLYLDSLKHLGIN
                     PKEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQAGGIDLDPISVEITYG
                     TERIAMYIQEVENVYDVEWTKGISYGEIHHRDEVEFSTYNFESSDYKMLTEQFGQFEK
                     ECENAAKNELSLPAYDYCLKCSHTFNLLDARGVIGVSERANYIGRVRNLAKMCAETYL
                     AQREKMGFPLLKNNPWTQNK"
     gene            2243..4366
                     /gene="glyS"
                     /locus_tag="AC159U_13215"
     CDS             2243..4366
                     /gene="glyS"
                     /locus_tag="AC159U_13215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3338056.1"
                     /translation="MDKDLILEIGTEEIPAGFLESSTARLKEIITKVFSDLNLGFSDI
                     AVYYTPRRITLRITGLPEKQEDITEHHTGPPKTIAYDNDGNPTKAAIGFAKKFGLDVN
                     KLKLVENDKGQFLGFTKNIKGKPTAKILKEQLPDIILKIPFPKSMRWRTSKISFARPI
                     RWILCLFNNKPLKFSIYNITSSNKSYGHRFLSPKSFKVETWNSYISQLENSYVVLDQH
                     SRAEIIAREVRIKAEELDGRVEEDTDLLQTVTNIVEYPVALTGTFEDEYLEVPPEVLV
                     SVMKYHQKYFPVYKKENPSISEGSVKSISDIRKINNLLPYFIFISGIKVDNPQVVIKG
                     NERVIRARFNDARFFLSEDTKQHLEKYIDKLKTVTYLSDVGSYYDKKTRLEKLSEYVS
                     NLPVNIDEQDIKRAAELCKADLVTQMVFEFPELQGTMGKYYALISGESPEVATAIEEH
                     YMPLTRDGELPSTGLGMFLSIIEKLDNVCSCFYAGLIPTGSADPYALRRQAIGIIRII
                     IDNKINLNINNLINSWIENCGLEIPLESKDKLRGQILDFFTERFRNFLLEEHHFDFDV
                     IDAALSVKFENILDSINVVQTITELKEKQDFESVTTAFKRVVNITKNTQQGTIDTSLF
                     LHDVEHQLYKQFRTTENEVTKDLESGDYQSVLAKIVQLRIPVDNFFDSVMVMDKNLQI
                     KNNRLSLLNEIKNLFFKVADFSKLN"
     gene            4486..5445
                     /locus_tag="AC159U_13220"
     CDS             4486..5445
                     /locus_tag="AC159U_13220"
                     /inference="COORDINATES: protein motif:HMM:NF040105.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3338057.1"
                     /translation="MITDNPYVALKEEMDNLVVGHEDVKMSLLLGIISREHIYIEGPP
                     GTAKTMLSEIISSAAQLQFFFYQLHRDTRLSEIIGDLVISKENTEEGEEIIKQKIVKG
                     GILTAEICLLDDISRAPGESLNVLLRILNERKFFNEDIPLLTAIATSNPTADEYYNEP
                     LDPANLDRFILQINAKGLSYGRKWDEISEVIKLYSKDSDKREVPERVTRKMLDTYYKK
                     LNSVKIPVEVQNLLKSFVMILIDKYGLNETNSLISDRTFFVKSLKILKSHALLHDRKS
                     CTPEDLRALRYMTAFRVPEEIHAMIDQIIDEVINQKKKPKKSK"
     gene            complement(5579..5731)
                     /locus_tag="AC159U_13225"
     CDS             complement(5579..5731)
                     /locus_tag="AC159U_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338058.1"
                     /translation="MTSGERTSSSSSFFSPLSAGLPSGSLLVFSSGGGINPGSCSILS
                     LSSFSA"
     gene            5826..6608
                     /locus_tag="AC159U_13230"
     CDS             5826..6608
                     /locus_tag="AC159U_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3338059.1"
                     /translation="MKVLEGNFQRSLALQAVHPGGSPRKWKRMTTFDEMEDIDPFEAV
                     FWSLYTSPSLPRVHRREKEVMGGEIAILRDVSTSMMGVYSEWSSSVVKGVIQLARSKN
                     MRVGYIEFNHKSFKHEKNGNFFTRDYNWMIESASNTDCSGNTNYEDALKDALDEFKGK
                     GLRNKHILFITDGIPTSGDCEVIKERERAKKLGVCIHSIFIGAKNFPRILEKISEETQ
                     GAQFIASKRADGIIRIERKDKNFTMPVIEKAHVDPFARAFKS"
     gene            complement(6605..7615)
                     /gene="mltG"
                     /locus_tag="AC159U_13235"
     CDS             complement(6605..7615)
                     /gene="mltG"
                     /locus_tag="AC159U_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3338060.1"
                     /translation="MSLRSIFLTLLILLVLCSAGALYLFNYYTEQFTFNSTAKVVEIQ
                     KGESVTKISKKLYDKKVIFNDKLFVLYVKINNLESGLKAGEYEFDKELTIKNVTDKLI
                     KGDVKLRKITIPEGLTLKEIAQIFERNKLFSASKFMALLDDKSFQEELLGNDIGSFEG
                     YLFPETYHYGKDVTAKEFIKIMVQMHNKVFSELKPHYSGENNLSDYEILKLASIIEKE
                     SGAGSELTTISSVFHNRLRIGMKLDSDPTIIYGMGDRYDGNIRKRDIEQYTEYNTYHI
                     PGLPPTPISNPGKAALHAAMNPDETQFLYFVSMGNGTHYFSKSYKDHQRAVYNYQIRK
                     RN"
     gene            complement(7638..8771)
                     /gene="proB"
                     /locus_tag="AC159U_13240"
     CDS             complement(7638..8771)
                     /gene="proB"
                     /locus_tag="AC159U_13240"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3338061.1"
                     /translation="MSDTERKEYLKKVHRVVIKIGSSVLTRENGELNENIFDLLASQI
                     SHIKSAGLEVIVVSSGAIAAGMKKLNIEQKPEDIVMKQAIAACGQSTLMGIYEQSFSK
                     YSQISAQVLLTHAGFSDRKRFITARKTIFKLLKMGIIPVINENDAVANEEIMVGDNDN
                     LAALVTSLIEGDLLILLTNIDGLYSADPTENKDAKLISLIKEVNGDIEKYAGKTHGKT
                     TTGGMVTKIEAVKSAAAFGVPTIIANGNDPSNISAIFSGDNIGTFFLPAMEKLTGRKH
                     WIAFAHKPLGKIFVDDGAKNAITSSGKSLLPSGITHVEGDFGIGELVVCVDSSGSEIA
                     RGLCSYSAADIKKLAGQKTSNIKKILGYKYGDEIIHRDDLVII"
     gene            8885..8961
                     /locus_tag="AC159U_13245"
     tRNA            8885..8961
                     /locus_tag="AC159U_13245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8919..8921,aa:Gly,seq:gcc)
     gene            complement(8963..9568)
                     /locus_tag="AC159U_13250"
     CDS             complement(8963..9568)
                     /locus_tag="AC159U_13250"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3338062.1"
                     /translation="MIKLYDHPLSGNCYKVRLLLGHLGLDYERETIDIFKGEHKTDTY
                     TELNPNQKIPTLDDDGFILWESNAILLYLANKHSPNSYISSDPNKYGLITQWLIFGKT
                     SVDPNLAIARFYKKFLGEGNYNKDELQKLHTAGGQMLSTLDQHLSRNEFLAGDYSVAD
                     MACFPYILVSPEGGFDLSKYSNVEDWIKRVESQDGFTKMED"
     gene            complement(9570..10064)
                     /locus_tag="AC159U_13255"
     CDS             complement(9570..10064)
                     /locus_tag="AC159U_13255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3338063.1"
                     /translation="MIREIENIFTDFSDYGCFACDPSNKLGLRMKFFADDETGEVFTK
                     MNMEKQFEGFPGILHGGIQCALMDEVAFWAMFDKLKKIGLTANIELSYKRKVETLKDI
                     EIRGKVGEINNRRVVVDVNILDEKGSVTASSKVTYLMPNRNTLYKLMCKEKFTEKFQQ
                     YIQD"
     gene            complement(10151..10597)
                     /locus_tag="AC159U_13260"
     CDS             complement(10151..10597)
                     /locus_tag="AC159U_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035091 - phosphatidylinositol binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PX domain-containing protein"
                     /protein_id="HIR3338064.1"
                     /translation="MRTIIDEVEIGGATESFVPAIITTSMSEAFEGLIGMDFMGNYSI
                     SIDNVNNYIVLQENPGNSDLPAGHSENWWRRSFAEFRAFQQEWKQVSELLNEMERKSR
                     INLNMTDSEVKDLKNLALWQHQESTKLLQRLEKYAQQNDVPQNWRN"
     gene            complement(10607..11050)
                     /locus_tag="AC159U_13265"
     CDS             complement(10607..11050)
                     /locus_tag="AC159U_13265"
                     /inference="COORDINATES: protein motif:HMM:NF025034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3338065.1"
                     /translation="MPLALLLLANPVFAQNIYEWTDENGVKHYSNTEAGVPEQYRKES
                     NSIKFGDNHNIGNSPQNDDFSNTDELINTFVGGGEVYKIPFTPFEGAARRIIIPVTIN
                     NDITAQLALDTGSPGMIISQSLAKKLGLYENNSGLLLSVASGIGG"
     gene            complement(11197..13632)
                     /gene="gyrA"
                     /locus_tag="AC159U_13270"
     CDS             complement(11197..13632)
                     /gene="gyrA"
                     /locus_tag="AC159U_13270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3338066.1"
                     /translation="MPGSSQIVPINIEAEMKESYISYSMSVIIGRALPDVRDGLKPVH
                     RRILFGMHEAGNAYNRPYKKSARIVGDVMGKYHPHGDSAIYDTLVRMAQDFSMRYQLI
                     DGQGNFGSIDDDPPAAMRYTEVRLRKITSEMLDDLDKETVDFVENYDGSSREPVVLPS
                     KIPNLLLNGSSGIAVGMSTNIPPHNLGELVSGVIAQIKNPDITVTELMTHIPGPDFPT
                     HGFISGRGAIRDAYETGRGIIKIRARAFIERQKKGDREVIIITELPYQVNKKRLIERI
                     VDLVKDEKIKGISDIRDESDRDGIRVVVELKRGEISEVILNQLYKHTPMDTSFGIIML
                     ALVRNQPKVLPLKDVISNFIDHRKEVVTRRTQYELRKAEERLHILEGLKIALDNIDEV
                     IALIRKSGSPADAKAGLVQKLSLTEIQAQAILDMRLQRLTALERDKILEERKELIEVV
                     KKLKEILAKEQLVLDIIIKELEEIKDKYADPRRTEIVDDIGDMSIEDLIADEDMVVTI
                     THDGYIKTTPLSIYRSQRRGGKGKTGMSTKDTDFVEDLFIASKHNYVLFFSNLGRCYW
                     LKVHEIPESGPTTRGKALVNLLNLAENEQVAAVLPVIEFKDDQYIIMATRGGTVKKTK
                     LMAYSKPRVGGIIALNIKDGDELIGARLTSGESEILLSTRNGQAIRFKEGDVRDMGRT
                     ATGVIGIRLGKDDVVVSLEVIENQNAQVLTVTEMGYGKRTQVTEYRLTGRGGKGVITL
                     KTNDKTGRVVGAFQVTDDMQIMIITTHGGKVIRMDASEISVYGRGTQGLRLIGLNEDE
                     RVAAVAKVIEREQ"
     gene            complement(13749..13821)
                     /locus_tag="AC159U_13275"
     tRNA            complement(13749..13821)
                     /locus_tag="AC159U_13275"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13786..13788),aa:Phe,seq:gaa)
     gene            13941..14861
                     /locus_tag="AC159U_13280"
     CDS             13941..14861
                     /locus_tag="AC159U_13280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR3338067.1"
                     /translation="MMQIGLTLPIRNDIDAKTNIEIGVEAEKLGFDSVWVSDHVVVPT
                     NHIGSFSEIFHDPFVLLSALAGVTKKISLGTSLIILPYRNPIVVAKMISTLDQLSDGR
                     VIFGVGTGWMKEEFDTLAVPFKERGKRSNEYIEIIKNLWTEENPEFDGEFFSYSDIKF
                     EPKSVQKPHPPILVGGNSEKAWQRAVLHGSGWQPTWYSPEKMEEEQEKLKEFASENDK
                     DLDNFFFTVRNRIALKHEKLSKDYTKELGENKPAYLFSGSVDSILADMEKFRSLGIEH
                     ILLDPVVDDLDDYSKVMEIISDEIIPAIKP"
     gene            complement(14868..15284)
                     /locus_tag="AC159U_13285"
     CDS             complement(14868..15284)
                     /locus_tag="AC159U_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338068.1"
                     /translation="MGLSPQKLTGLIQETKRAAAALDKVGDYAKLMKKELNDLPGESK
                     KSVNSISRAVGRIRKNIDELTNNINGKLNNMELYDEDIEEAANKLLLFHSSVDEVLNW
                     AETQLQNHKKNSYWGKYWKGVYEVVSKRKEQQKGQQ"
     gene            complement(15308..16111)
                     /locus_tag="AC159U_13290"
     CDS             complement(15308..16111)
                     /locus_tag="AC159U_13290"
                     /inference="COORDINATES: protein motif:HMM:NF018963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HIR3338069.1"
                     /translation="MKIKIICIFTAVLCSSVFFASTIESEEKAKIDYGSFTEPKLQQI
                     VQASSFFKDEGEKIDYISSKFLGTAYKDHTLTGDINTPEIFTINLKGVDCFTYLDYVE
                     GLAISKSFNQFGDSVKKIRYQNSKVDFKKRNHFFSDWPVYNKERIKDVTKDIGGDKAK
                     STKKFLNKKKDGSLYLPGIAVVEREIAYIPSESIDDDILNKMNTGDYAGIYTDLDGLD
                     VSHTGIIIKKEGKTFLRHASSKDEYMKVVDEDLIGYFSTKAGIIIYRAN"
     gene            complement(16144..17514)
                     /locus_tag="AC159U_13295"
     CDS             complement(16144..17514)
                     /locus_tag="AC159U_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3338070.1"
                     /translation="MITVLIVDDEPGVRESMSLILKMEGYSCDTVRDAVHALKLLGSG
                     KKYDFIISDIKMPDMDGLEFLAELKKQQVDSIIIMISAYGTIESSIKAIKFGASDYIN
                     KPVNIDELILRMKMSEERKKLKRENVALKKELRKEVGFEDIVCKDAKMKGIIELASKV
                     SDYKTTVLITGESGTGKELVARSIHANSSRKDKPFVAVNCAAIPENLLESELFGYEVG
                     AFSGAAKSKPGLFEQADGGTLFLDEIGEFPIALQPKLLRTLQDEEIRRLGGTKTNKID
                     VRILAATSRDLTEQIEKSEFRADLFYRLHVMPIEIPPLRQRQDDIAVLVDYFIEKYNE
                     KLGSNVKGVTKKVMDKLISFPWPGNVRELENLIEKTMILTQSDIIDEVEIVQRDEQDD
                     PKLWIGSLKLDEAMNKLEKLYIEKALSQTGGNRTKAAETLGISRRGLLYKIKEYGLGS
                     EDEKED"
     gene            complement(17521..>18286)
                     /locus_tag="AC159U_13300"
     CDS             complement(17521..>18286)
                     /locus_tag="AC159U_13300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3338071.1"
                     /translation="HQQIEESKGKLEENITALQRSNEVIKSTQKELIASEKFASLGKL
                     SAGIAHEIGNPLSAITGYVDVLNKSTDLSSEDRKRFLDNIKNEVSRIDRIIKTLLEYA
                     RAKEFEIKKVNLNDIIADSVKVLQTQGIFNDIDLQINLDRNLKMVDADPHQLSQVIIN
                     LLLNAVDATEQKGQIIITSQNEADAVNMSIKDNGMGISEENIDKIFDPFFTTKEPGKG
                     TGLGLSVSARIVQHFGGQLKVQSKQGEGSVFSIRFR"
BASE COUNT         5578 a         3718 c         3330 g         5660 t
ORIGIN      
        1 ttactaatat tatttaaatt taatgaatgc caacaataaa atagtggttg taggagccgg
       61 tcttgcagga actgaggctg catatcagat tgcaaaaaga aatattgaag tagacctata
      121 tgaaatgcgt cctcataaaa agagtcccgc gcacaataca gataagtttg cagagcttgt
      181 atgcagtaac tcccttaagt ccaactatct tgaaaacgca agcgggattt taaaagaaga
      241 aatgagaaga ctcgattcgc ttatcattaa atcagcagat aaaacaagtg tgccggcagg
      301 aaaagctctt gcagtcgaca gaactagatt cgcagaccac ataaccagtg ttttagaaca
      361 aaatccttat ataaatatta aacgcaggga atttgataaa ataccagtaa ccatcgggac
      421 acctgtaatc attgcctccg gccccctaac atctaaagac ctatcaaatg aattaagtaa
      481 aatcacctgc acagactatc tttatttttt tgatgcaatc tcacctataa tagaagctga
      541 aagcattgat tttgacaaag cgttttttgc atcaagatat gataaaggtg aagatgagca
      601 gggggattac ctaaactgtc cgcttgatag agacgagtat tacagactta ttgatgatat
      661 aatttcggca gaaaatattg aagtaaaaga gtttgatgat gtgaaatatt ttgaaagctg
      721 ccttcctgta gaagttattg ccggcagggg caaagattct ttgaggtttg ggcctatgaa
      781 ggcaaaaggc ctaacagacc caaaaacatc caaacgccca tatgctgtac tgcagcttag
      841 aaaagaaaat tatgcaggtt ctatgtacaa catggtaggt tttcaaacga ggcttaaata
      901 ctcagagcaa aaaagggtat tctcaaacat tagcgggctt gaaaatgccg agtttctaag
      961 atacggaagc atgcacagaa atacttatat aaactctcca aaacttctcc agcccacgct
     1021 tcagctaaaa aatcacccaa acatttttct tgcgggtcag attgtggggg ttgaggggta
     1081 tgtagaatcg gcagccatgg gattaatagc gggtataaat gccgttaagc tcactcaggg
     1141 ttgtgaaccg aaaatacctt gtgagcaaac ttccatcggg tcgttactaa aatatattac
     1201 agactcaggc ctaaaagaat ttcagcctat gaatataaat ttcggtcttt accctgaagt
     1261 agaaaaaaaa tatactaaaa ctgaaaaaag aaggtatatt gctgaacggg ctttatctaa
     1321 aataactgaa tatataaact aatttttaca agatgacatt tcaaaagctt ttattagaat
     1381 tgcaaaacta ctggtctaag aaaaactgca taatttacca gccctatgac cttgaaaaag
     1441 gagccggaac ttttcatccc gcaacttttt taagatgcct tgggccagaa ccctggcagg
     1501 tagcttatgt agagccatcg agaagaccca cagacggaag gtatggagat aatccaaaca
     1561 gactccagca ctactatcag tttcaggtaa taatcaaacc ttctccggat aatatccagg
     1621 atttatatct ggacagtcta aagcacttgg gaattaaccc aaaggagcat gacataagat
     1681 ttgtggagga tgactgggaa tctcccaccc ttggcgcctg gggacttgga tgggaagtat
     1741 ggcttgatgg aatggaaatt actcagttta cctatttcca gcaggcgggc ggtatagacc
     1801 tcgatccaat ctcggtagag attacctatg gaacagagag aattgccatg tacattcagg
     1861 aagttgaaaa tgtatatgat gttgaatgga caaagggaat atcatacgga gagattcacc
     1921 acagagatga agtcgagttt tctacatata attttgaaag ctcggattac aaaatgctta
     1981 ctgagcagtt cggccagttt gaaaaggaat gtgaaaatgc agcaaagaat gaactttctt
     2041 tacctgctta tgattactgc cttaaatgct cccatacttt caaccttctc gacgcaagag
     2101 gagtgatagg ggtttcagag cgtgcaaact atattggaag agtgagaaac cttgccaaga
     2161 tgtgtgcaga gacctatctg gcccaaagag aaaaaatggg gtttccactt ttaaagaata
     2221 atccctggac acagaacaaa taatggacaa agacttgata ctagaaatcg gaaccgaaga
     2281 aatacctgct ggttttttag aaagttccac tgcaagacta aaagaaataa tcacaaaggt
     2341 tttttcggac ttaaacctgg gatttagtga tattgcagtt tactacacac cccggcgaat
     2401 tacacttagg attacgggcc tgcccgaaaa gcaagaagat ataacagaac accatacagg
     2461 acctccaaag accatagcat atgataatga tggaaacccg acaaaggcag caatcggttt
     2521 tgcaaagaaa ttcggtcttg atgttaataa attaaagctt gttgaaaatg acaagggaca
     2581 gtttttggga ttcaccaaga acattaaggg taaacctact gccaagatac taaaagaaca
     2641 actaccagac ataatattaa aaataccctt tccaaaatcc atgaggtggc gtacgagcaa
     2701 aatatcgttt gcaaggccga taagatggat tttatgcctt tttaataata agccattaaa
     2761 attcagcatt tataacataa caagttcaaa caagtcttat ggccacaggt tcctaagtcc
     2821 aaaaagtttt aaggttgaga catggaatag ctacatttcc cagcttgaaa attcatatgt
     2881 tgtactcgat cagcacagcc gcgcagaaat aattgccagg gaagtcagga ttaaggcaga
     2941 ggaattagat ggaagagtag aagaagatac agacctgcta caaactgtta caaacatagt
     3001 agaatatccc gtggcgctta caggaacctt tgaagatgaa tacttagaag ttccacccga
     3061 ggttttagtg agtgtgatga aatatcacca aaaatatttc cctgtttata aaaaagaaaa
     3121 tccatccata agtgaaggtt cggtaaaatc tatttcagat atcaggaaaa taaataacct
     3181 gctgccttat ttcatattca tatccggaat aaaagttgat aacccccaag tggtgataaa
     3241 aggaaatgaa agggtaataa gggcaaggtt taatgatgcc agattttttc tttctgaaga
     3301 cacaaaacaa caccttgaaa aatatattga taaactaaaa actgtcacat atctgtccga
     3361 tgtcggcagt tactatgaca agaaaacaag attagaaaaa ctatcagaat acgtctctaa
     3421 tctacccgtc aatattgatg agcaggatat caaaagggca gccgagcttt gcaaggcaga
     3481 ccttgtaaca cagatggttt tcgaattccc ggagcttcag ggaacgatgg gaaaatacta
     3541 tgctctaatc tcaggagaat ccccagaagt agcaactgct atcgaagaac attatatgcc
     3601 tttaacaagg gacggagagc ttccatccac agggcttgga atgtttttaa gcataataga
     3661 aaagttagat aacgtatgca gctgttttta tgcaggactc attcccacag gaagcgcaga
     3721 tccatatgct ttaagaagac aggcaatcgg gattataaga ataatcattg ataataaaat
     3781 taatcttaat ataaacaatt taataaatag ctggatagaa aactgtggtc tggaaatacc
     3841 cctggaaagc aaagataagc tacggggcca aatccttgat ttctttacag aaagatttag
     3901 aaacttcctg ttagaagaac atcattttga ctttgatgta attgatgccg cattatcagt
     3961 aaagtttgaa aacatactgg attccatcaa tgttgtacaa accattacag aattaaaaga
     4021 aaaacaggac tttgaatcgg taaccacagc ttttaagaga gtagtaaata ttaccaaaaa
     4081 cactcagcag ggaactatag acacatcgct atttcttcac gatgtagaac accagttgta
     4141 taaacaattt agaaccacag aaaatgaagt cacaaaagat ttagagtccg gcgattatca
     4201 aagcgtactt gcaaagatag tccagctgag aattcctgta gataattttt tcgacagtgt
     4261 gatggttatg gataaaaacc tgcaaataaa aaataatagg ctatccttgt tgaatgaaat
     4321 caaaaatctg ttttttaagg ttgctgattt ctccaaattg aattaattgc ttgtataagt
     4381 tttattaatt ttggattaaa ttatattttt gttgctaaga aatatgtatt tatgtaatat
     4441 ctaagactaa tttagttaaa tacattcagg aggcactcct acacaatgat tactgacaat
     4501 ccttatgtag ctttaaaaga agagatggac aaccttgtgg tcgggcatga agatgtaaag
     4561 atgtccttac ttcttggaat aatatcaaga gaacatattt atatcgaggg tcctccggga
     4621 acagccaaga ccatgctctc agaaattatt tcaagcgctg cccagctaca gtttttcttt
     4681 tatcagcttc atagagatac caggctatcc gaaataatag gagatttggt aatatcaaaa
     4741 gaaaacactg aagaaggcga agaaataatt aaacaaaaaa ttgtaaaggg cggcatatta
     4801 accgctgaaa tctgtcttct agatgatata tcaagagcac cgggagaatc actgaatgta
     4861 ctgcttcgta tattaaatga aagaaaattt ttcaatgagg atattcccct tctaacagca
     4921 atagctacaa gtaaccctac agccgatgaa tattacaatg aacccctcga tcctgctaac
     4981 cttgacagat ttatcctgca gataaacgca aaggggcttt catacggaag aaaatgggat
     5041 gaaatatctg aagtaattaa gctatattca aaagactcag acaagcgcga ggtgcccgaa
     5101 agagtcacaa gaaaaatgct ggatacgtat tataagaaac taaactctgt aaagattcct
     5161 gtagaggtac agaatctgct taagagtttt gtaatgatat taattgacaa atacggttta
     5221 aacgaaacca attcactcat atctgacagg acttttttcg taaaatcctt aaagatttta
     5281 aaatcacatg ctctgctcca tgatagaaaa agctgcaccc ctgaagacct gagagccctt
     5341 agatatatga ctgctttcag agttcctgaa gaaattcatg caatgatcga ccagattatt
     5401 gatgaggtga ttaatcaaaa aaaaaagccg aagaagagca aatagctaat actaatcagg
     5461 aagcaaactt tggaaatgac gactctagag atgctgaaga agacagagaa cagattgctc
     5521 cagatgaagc agaggagcat acagacgatc agcttcatga agccaaaaag accttttttc
     5581 aggcgctaaa agaacttaag gacaatatag agcaggaccc cggattaatc cctccacctg
     5641 aagaaaaaac caagagcgac cccgatggca gaccagccga cagtggtgag aaaaatgaag
     5701 acgatgaaga agtacgctca cccgaggtca tttatcctag caagtcagag tccccgggag
     5761 atgaagatga atactgggta ggtggcaaga aaaacctaag tactgccgat aacataaaaa
     5821 tgataatgaa ggttttagaa ggaaactttc aaagaagctt agctctacag gctgtccatc
     5881 cgggtggttc tccaagaaag tggaagagaa tgaccacatt tgatgaaatg gaagatatcg
     5941 atccatttga ggcagtattt tggtcattat atacctcacc cagtcttccc agggttcata
     6001 gaagagaaaa agaggttatg ggcggcgaga tagcaatcct aagagacgta agcacctcaa
     6061 tgatgggagt gtatagtgaa tggtcatcat cagttgtaaa gggagtaatt cagcttgcaa
     6121 ggtcaaaaaa catgagggtt ggttatattg aattcaacca taaatcattc aagcatgaaa
     6181 aaaacggcaa tttctttacc agggactaca actggatgat tgaaagcgct tcaaatacag
     6241 actgctctgg aaacacaaac tacgaagatg cacttaagga tgctcttgat gaatttaaag
     6301 gtaaggggct tagaaacaaa catattcttt tcataacaga cggcatacct acatcagggg
     6361 actgtgaagt aataaaagaa agagaaaggg caaaaaaact tggcgtatgt attcactcta
     6421 tatttatcgg cgcaaaaaac tttccaagaa ttctggaaaa aatttcggag gaaacccagg
     6481 gtgcacagtt tatagcgtca aagagagccg atggaattat aagaatcgag agaaaggaca
     6541 agaactttac aatgcctgta atcgaaaagg cacacgtaga tccgtttgca agggctttta
     6601 aaagctagtt tctctttctg atctgataat tgtagactgc tctttgatgg tctttatagc
     6661 tcttggaaaa atagtgggtt ccatttccca ttgatacaaa gtataaaaat tgggtttcat
     6721 ccgggttcat tgccgcatgc agggctgctt ttcccggatt ggatattgga gtaggtggaa
     6781 ggccgggtat gtgataggtg ttatattctg tatattgctc gatatctctt tttcttatat
     6841 taccgtcata tctgtctccc atgccgtata tgatggtggg atcactatca agcttcatcc
     6901 caatcctcag cctgttatga aataccgacg atatagttgt aagttcactg ccggcacccg
     6961 attctttttc aattatcgag gcaagtttta agatttcata atcgcttaga ttattttctc
     7021 cagagtaatg aggctttagt tcagaaaaga ctttattgtg catttgcacc atgatcttta
     7081 taaactcttt agcagttacg tcctttccat agtgataggt ttcaggaaat aaatatccct
     7141 caaaagagcc gatatcattt cccagcagct cttcttggaa gctcttgtca tctaataaag
     7201 ccataaactt gcttgctgaa aataatttat tcctctcaaa tatttgagct atttctttta
     7261 aagtaagccc ctcgggtata gtaatcttcc gaagcttcac atctcctttt ataagcttgt
     7321 cggttacatt tttaatcgta agttctttat caaactcata ttctccggct tttaaacctg
     7381 attccagatt gtttatcttt acatatagaa caaagagttt gtcattgaaa attacttttt
     7441 tatcataaag cttcttggat attttggtaa cactttcccc cttttgaatc tcaacaactt
     7501 tagcggttga attaaatgta aattgttctg tgtaataatt gaagagatat aatgcccctg
     7561 ctgagcacaa tactaataga attaataaag taagaaatat agaccttaat gacatatata
     7621 atttattact aattaatcta aattattacc agatcgtccc tgtgaataat ttcatcaccg
     7681 tatttataac ctagtatttt tttaatattg gaagtttttt gtcccgcaag tttttttatg
     7741 tctgcagcac tataagagca tagaccccgg gcaatttcac tgccgctgct atcaacacat
     7801 actacaagct caccaatacc aaaatcacct tctacatgcg ttattccgga aggaagcagg
     7861 ctctttccag atgaggtaat agcatttttt gccccatcat ctacaaagat tttaccaagg
     7921 ggcttatggg caaaagcaat ccagtgcttt cttcctgtaa gcttttccat agcaggaagg
     7981 aaaaatgtac caatattatc accactaaat attgccgaaa tatttgatgg atcattgcca
     8041 tttgcaatga tagtcggtac tccaaatgca gcagcgcttt ttacagcttc aatcttagta
     8101 accataccac ccgtagtggt cttgccatgg gttttacctg catatttttc tatatcacca
     8161 tttacttctt ttataaggct tatcagtttt gcatccttat tttcagtagg atcagcgctg
     8221 taaagcccgt caatattggt tagtaaaatc agcagatcac cttcaataag gcttgttaca
     8281 agtgcagcaa gattgtcatt atcaccaacc ataatctctt cgtttgctac tgcatcgttt
     8341 tcatttatta ccggtataat ccccattttg agaagcttaa aaatggtttt ccttgctgta
     8401 ataaaccttt ttctatcaga aaacccggcg tgagtaagga gcacctgtgc tgaaatctgg
     8461 ctgtattttg aaaaagattg ttcataaatc cccattaacg tgctctgacc gcatgctgct
     8521 atggcctgtt tcatcacaat atcctcaggc ttttgctcaa tattgagttt tttcatccct
     8581 gcggctattg cccctgatga aacaacgatt acctctagcc cggcagattt aatatgggaa
     8641 atttgtgaag ccagtaaatc aaatatgttt tcattcaact caccgttttc acgagtcagc
     8701 acgctgctgc ctattttaat tactaccctg tgaacctttt ttaagtactc ttttctctct
     8761 gtatcgctca ttctatttag ctttatttct taggttagaa tttgttatta ttaattaaac
     8821 agattatgta aaataatcca atcttgacaa gacaattcag tctggctatc tttaacactc
     8881 ctaagcggga atagctcagt tggctagagc gtctgcttgc caagcagaag gtcgcgggtt
     8941 cgaatcccgt ttcccgctcc atttaatctt ccatttttgt aaatccatct tgtgattcta
     9001 ctcttttaat ccaatcttcc acattgctat atttactaag atcaaagccg ccttcgggag
     9061 ataccaatat gtatggaaaa caagccatat cggctactga gtaatctcct gcaagaaatt
     9121 cattccggct taaatgctga tccagtgttg agagcatctg ccctccggct gtatggagct
     9181 tttgaagttc gtctttgtta taattgccct ctcccaaaaa ttttttataa aaccttgcaa
     9241 tagcaagatt tggatcaact gaagttttac caaaaatgag ccactgagtt ataagaccat
     9301 atttattagg atcagaagat atatagctgt ttggagagtg cttgtttgca agatagagca
     9361 aaattgcatt tgattcccag aggataaatc cgtcatcatc cagtgtagga attttctgat
     9421 tagggtttag ctctgtataa gtatcagtct tatgctcgcc cttaaatatg tctattgttt
     9481 ctctttcata atcgagcccc agatgaccaa gaagaagtct aactttatag caattacctg
     9541 aaagcggatg gtcgtataat tttatcatct tagtcctgta tatactgctg gaatttttcg
     9601 gtaaattttt ctttacacat gagtttataa agagtatttc tgttgggcat taaataagta
     9661 actttgctgg acgctgttac actacccttt tcatccaaaa tatttacgtc tactaccact
     9721 ctcctgttgt tgatttcccc gactttaccc cttatttcaa tatcttttaa tgtttctacc
     9781 ttgcgtttgt aactaagttc gatatttgct gtaagtccaa ttttttttaa tttatcaaac
     9841 attgcccaga aagccacctc gtccatcagc gcacattgta tgcccccatg caatatcccc
     9901 gggaacccct caaactgctt ttccatattc atctttgtaa atacctcacc cgtttcatca
     9961 tcggcaaaga atttcattct caaccccagt ttatttgacg gatcacaggc aaaacagcca
    10021 taatcagaaa agtctgtaaa tatattttct atttctctta tcattgtata tcaaaatatt
    10081 tttgttacag gatttttggt acaggataat aactgaaaat cggtcttttt aaatttagac
    10141 tcaaagaaat ttaatttcgc cagttctgcg gcacgtcatt ttgctgggcg tatttttcca
    10201 gtctttgaag cagttttgta gattcctgat gctgccataa tgcaaggttt ttaagatctt
    10261 ttacttcact atctgtcata tttaagttta ttctcgattt tctttccatt tcatttaaaa
    10321 gctcgcttac ttgtttccac tcctgctgaa aagctctgaa ctctgcaaac gatctcctcc
    10381 accaattctc tgaatgtccg gcgggaaggt cagaatttcc cgggttttcc tgaagaacaa
    10441 tatagttgtt aacattatct atgcttattg agtagttgcc cataaagtcc ataccgataa
    10501 gcccctcaaa ggcttcagac attgatgtag taattattgc aggcacaaag ctctctgttg
    10561 ccccgccaat ttctacttca tctattattg tcctcatcac atttacctac cccccaatac
    10621 cacttgccac gctaagaaga agaccactat tattttcata aagccccagc ttttttgcaa
    10681 gactttgcga aatgatcatc cccggggagc cagtatcgag tgcaagctgg gccgtaatat
    10741 cattgttaat agttaccgga attattattc ttcttgctgc gccttcaaaa ggcgtaaatg
    10801 gaattttgta aacttcgccc ccgcccacaa aagtatttat cagttcatct gtgtttgaaa
    10861 aatcatcatt ttgaggggag ttacctatat tatggttatc gccaaatttt attgagtttg
    10921 attccttgcg gtattgttca ggtacaccgg cctcggtgtt ggaatagtgt tttaccccgt
    10981 tttcatctgt ccattcatag atattctgag caaaaacagg gtttgctaat agaagtaatg
    11041 caagaggcaa aagtaagaat aaattacccg taaatattct aaaaaggctg ttataaaaaa
    11101 atccccacat ggaataaaag tataatagca agaagcaaag attgccacaa aaacttatta
    11161 ttttggcttt aacaagttag aaatgcagta aataaattac tgctcgcgtt caataacctt
    11221 ggccacagca gcaactcttt catcttcgtt aagacctatg agccttagac cctgagttcc
    11281 tctgccgtat acgcttattt cagatgcatc cattctaatg acttttccgc cgtgtgtggt
    11341 aattatcatg atctgcatgt catctgtaac ctgaaaggcg cccactaccc tgccagtctt
    11401 atcatttgtc tttagtgtta ttactccctt accgcctctt cctgtaagcc tatattctgt
    11461 aacctgtgtt ctcttaccgt atcccatctc tgttactgtg agcacctgtg cattttggtt
    11521 ttctattact tcgaggctca ctacaacatc gtccttacca agccttatgc ctataactcc
    11581 tgttgcggtt ctgcccatgt ctcttacatc accctctttg aacctgatgg cctgaccatt
    11641 tcttgttgaa agcagtatct cactctcacc gctggtaagc cttgcgccta taagctcatc
    11701 gccatctttt atattcaggg ctattatgcc acccactctt ggctttgagt aagccatgag
    11761 cttagtcttt ttaacagtac cgcctcttgt tgccattatg atatattgat cgtctttaaa
    11821 ctctattaca ggcagtacgg ctgcgacttg ttcgttctct gccaaattga gcaggttcac
    11881 aagcgctttt cctcttgtag taggaccgga ttcaggtatc tcgtgtactt ttagccagta
    11941 acaacgtcca aggttgctaa aaaacagcac atagttgtgc ttggatgcta taaacaggtc
    12001 ttcaacaaaa tcggtgtcct ttgtagacat tccggtctta cccttcccac ctcttctctg
    12061 gcttctgtag atgctaagag gagtggtctt gatataaccg tcatgagtta tagttacaac
    12121 catatcctca tcggctatga ggtcttcaat ggacatatcg ccaatgtcat ccacaatctc
    12181 agttcttctg ggatctgcat acttgtcctt aatttcttca agctctttta tgatgatatc
    12241 gagcacaagc tgttcttttg ccagtatctc ttttagtttc tttacgactt caataagctc
    12301 ttttctttct tctagaattt tatctctttc taaagctgta agcctctgca gtctcatatc
    12361 cagtatggcc tgggcctgaa tctctgtaag ggaaagtttt tgtacaaggc ctgcctttgc
    12421 atcagccggg cttccggatt tgcggattag cgcaattact tcatcaatat tatcgagcgc
    12481 aatctttaag ccttccagta tatgaagtct ttcttcggct tttctaagct catactgggt
    12541 tcttcttgtg acaacctctt ttctatgatc aataaaatta cttattacat cttttagagg
    12601 aagcaccttt ggctgatttc ttacaagtgc cagcatgatg attccaaaag atgtgtccat
    12661 cggggtgtgc ttgtagagct ggtttagaat tacttcggat atctcacccc tcttaagctc
    12721 aacgactacc cttatgccgt ctctgtcaga ctcatccctt atatcggaaa tgccttttat
    12781 cttttcgtct tttacaaggt caactatacg ttcaatgagc ctcttcttgt taacctgata
    12841 gggaagctct gtaataataa ttacttctct gtcgcccttt ttctgccttt caataaatgc
    12901 acgggcacga attttaataa ttccacgccc tgtttcataa gcgtctctta ttgcacccct
    12961 tccggaaata aaaccatggg ttggaaaatc aggtcctggt atgtgggtca taagctcggt
    13021 taccgttata tcaggatttt ttatctgggc tataacacca cttacaagct cgcctaaatt
    13081 gtggggagga atgttggtag acataccgac tgcaataccg gatgaaccgt ttagaagaag
    13141 atttggtatt ttcgagggta ggactacggg ttctctggaa ctgccgtcat agttttcaac
    13201 aaaatctacg gtttctttat cgagatcatc gagcatttca gaagtgatct ttctgagtct
    13261 cacctctgtg taacgcattg ctgctggggg atcatcatca atcgagccga aatttccctg
    13321 accgtctatt aactgataac gcatcgaaaa atcctgcgcc attcttacta atgtgtcata
    13381 gattgcagag tcgccatggg gatgatattt acccataacg tctcccacga ttcttgcaga
    13441 cttcttgtaa ggcctgttat aggcatttcc ggcttcgtgc atgccaaaga ggattcttct
    13501 gtgtaccggt ttcagcccgt cccttacatc tggtagcgct cttccgatga ttacactcat
    13561 cgaatagctg atgtaagatt ccttcatctc agcttcaata ttaattggta ctatttggga
    13621 cgaaccgggc atagaaacct ccaaaaatta aataacttaa aacagaatat atgacactgt
    13681 atatagtgtc aaaaagagtt ttacattcaa tatattgtat ctgaaagtat taaaaagtca
    13741 ttaaaatatg cccaggggcg gaatcgaacc accgacacgt ggattttcag tccactgctc
    13801 taccgactga gctacctggg cactaaaaac taatatttca ttcaaagatc aattcgaaaa
    13861 tgaaggctta ttctacccta gtcataaagc actcgcaagg attaactttg ctaatttaaa
    13921 taaaaggata tacttaagaa atgatgcaga tagggcttac acttccaatt agaaacgata
    13981 tagacgcaaa gaccaatatt gagatcgggg tggaagctga gaagctgggg tttgactctg
    14041 tctgggtaag cgatcatgtt gttgttccaa caaaccacat aggaagtttt agtgagatat
    14101 tccacgatcc gtttgtgctg ctttctgcac ttgcaggagt aaccaagaaa atcagtctcg
    14161 gtacaagcct tatcatactt ccatatagaa accccatcgt ggttgccaag atgatttcca
    14221 cattggatca actctcagat ggcagggtaa ttttcggagt tggaacaggc tggatgaaag
    14281 aagagtttga tacccttgcg gttccattta aagagagggg aaagaggtct aatgaatata
    14341 ttgaaattat caaaaatttg tggactgagg aaaatcctga attcgatgga gaatttttta
    14401 gttatagtga tataaaattt gaacccaaat cagttcaaaa gccccaccct ccaatactcg
    14461 tgggtggcaa cagtgaaaaa gcatggcaaa gggcagtgct tcatggatca ggctggcagc
    14521 ccacctggta cagcccggaa aagatggaag aggaacagga aaaactaaaa gaatttgctt
    14581 ccgaaaacga taaagatctg gacaactttt tctttaccgt aagaaacagg attgctttaa
    14641 aacatgaaaa actatctaaa gattatacta aagagcttgg agaaaataaa ccagcatatc
    14701 tcttttctgg ttcggttgat tctattttgg ctgacatgga gaagttcaga agcttaggaa
    14761 tagaacacat actactcgac ccggtagtag atgatctgga tgactattct aaagttatgg
    14821 agataatatc agatgagatt atccctgcta ttaaacccta atattattta ttgctgaccc
    14881 ttttgctgtt cttttctttt tgatacaact tcataaacac ccttccaata ctttccccag
    14941 tatgagtttt tcttgtgatt ttgcagctga gtctctgccc agttcagcac ttcatctacg
    15001 cttgaatgaa agagaaggag tttatttgca gcctcttcaa tatcttcatc atatagctcc
    15061 atattgttca gtttaccgtt aatattgtta gtaagctcat caatgttctt gcgaattctc
    15121 cctaccgctc tgcttattga attaactgat ttcttggatt ccccaggaag atcgtttaat
    15181 tcttttttca ttaactttgc ataatccccg actttatcca aagcagcagc agctcttttt
    15241 gtttcctgaa tcagtcctgt aagtttttgc gggctaagtc ccattgttca cctcactata
    15301 ataataatta attagctcta tagatgataa tacctgcttt agtagaaaaa tacccaatca
    15361 aatcctcatc aactactttc atatactcat ctttagaaga tgcatgtcgt aagaaagttt
    15421 taccttcctt tttgataatg ataccggtat gtgatacatc cagtccatca aggtctgtat
    15481 aaatcccggc atagtctcct gtattcattt tatttagaat gtcatcatca atcgactctg
    15541 atggtatgta tgcaatttcc cgctcaacca cagcaattcc aggcaaatat aatgacccgt
    15601 ctttcttttt gtttagaaac ttcttggtag acttagcttt atccccacct atatcttttg
    15661 ttacatcctt aatcctttct ttattgtaaa caggccagtc actgaagaag tgattacgtt
    15721 ttttaaaatc aactttggag ttttgatatc tgattttttt aacactatct ccaaattggt
    15781 taaagctttt ggatatagct aaaccttcta cataatcaag atatgtaaaa cagtccaccc
    15841 cttttaggtt tattgtaaat atttcaggtg tgttaatatc cccggtaagc gtatggtctt
    15901 tataggctgt acccagaaat ttcgaagata tatagtcaat cttctctcct tcatctttaa
    15961 agaaagaaga agcttgcacg atctgctgta gttttggttc tgtaaaactg ccgtaatcta
    16021 ttttagcttt ttcttcagac tcaatggtac tagcaaagaa tacagaagaa caaagaacag
    16081 cggtgaagat acatataatt ttaattttca tgttattagg ttaacaaata atattatgta
    16141 tgctcagtct tctttttcat cctcactacc taatccatac tcttttatct tatagagcag
    16201 ccctcttctg cttatgccca atgtttcagc ggctttagtt ctgttgcccc cagtctgaga
    16261 cagagctttt tcaatataca gcttctcaag cttattcatc gcttcatcaa gcttaagtga
    16321 tcctatccaa agctttgggt catcctgctc gtctctttgt acaatttcga cttcatcaat
    16381 aatatcactc tgggtgagga tcatggtttt ttctatcagg ttttcaagct cccttacatt
    16441 ccctggccag gggaaagaaa taagcttatc catcaccttt tttgtaacac cttttacatt
    16501 tgatcccagt ttttcattat acttttctat aaaataatcc actaacactg caatatcgtc
    16561 ttgtctttgc ctaagcggcg gaatttctat gggcatcacg tggagtctgt aaaaaaggtc
    16621 tgccctaaat tcactctttt ctatctgctc agttaaatcc ctcgatgttg cagccagaat
    16681 tcttacatca atcttgtttg ttttagtccc ccctagcctt ctgatctcct catcctgcag
    16741 ggtccttaaa agctttggct gtaaagctat gggaaattct ccaatctcat ccaagaacag
    16801 cgtgccgccg tcggcttgtt caaaaagccc gggtttggat tttgcagcac cgctaaatgc
    16861 accgacttca tatccaaaca gctcactctc aagtaagttt tcgggaatcg ctgcacagtt
    16921 tacggcaaca aacggtttat ccttccttga gctgttagca tgaattgatc ttgcaacaag
    16981 ctccttacca gtcccactct ctcctgttat aagcactgta gttttataat cacttacctt
    17041 cgatgcaagc tctatgatcc ctttcatttt agcgtcttta caaacgatat cttcaaatcc
    17101 aacctctttt ctgagctctt ttttaagtgc tacattttcc cttttgagct tcttcctttc
    17161 ttcagacatt ttcatgcgca atatgagctc atcgatatta acgggcttat ttatatagtc
    17221 cgatgcgcca aacttgattg cttttataga gctttcaata gtaccgtatg ctgaaatcat
    17281 aataataatt gaatcaactt gttgtttttt taattcggcc agaaactcaa gcccgtccat
    17341 atcgggcatt ttaatatcag atatgataaa gtcgtatttt ttgccggagc caagaagttt
    17401 aagagcatga actgcatccc gtacagtatc acaactgtag ccctccatct ttaatattaa
    17461 agacatggac tctcttactc caggctcgtc atctacaatt aatactgtga tcataatctt
    17521 ttatctaaac cttatactaa aaacagatcc ttctccctgc ttactttgca cttttaactg
    17581 cccaccaaaa tgctgcacta tccgagctga aaccgacagg ccaagtcccg ttccttttcc
    17641 aggctctttt gtagtaaaaa agggatcaaa tattttatct atattttctt cggaaattcc
    17701 cataccgtta tcttttatac tcatatttac cgcatcagcc tcattttgag atgtgataat
    17761 aatttgtcct ttctgctctg tggcatcaac cgcatttagc aataggttta ttataacctg
    17821 agagagttga tggggatcag catctaccat cttcagattc cggtcaagat ttatctgaag
    17881 gtctatatca ttgaatatcc cctgagtctg cagaacttta accgaatcag ctattatatc
    17941 atttaaattt actttcttta tctcaaattc ttttgccctt gcatattcca gaagcgtttt
    18001 aattattcta tcaatcctgg agacttcatt tttgatatta tcaagaaacc gttttctatc
    18061 ttcagagctc aaatcggtgg atttatttag tacatccaca taacccgtga ttgcgcttaa
    18121 aggattgcct atttcatgtg ctatacccgc agagagctta ccaagtgagg caaacttttc
    18181 agacgctatt aactcctttt gtgtactctt tataacctca ttagatcttt gcagtgcagt
    18241 aatattttcc tcgagcttgc ccttactttc ttcaatttgc tggtgc
//
LOCUS       DBNGSV010000373         7109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1659405, whole genome shotgun sequence.
ACCESSION   DBNGSV010000373 DBNGSV010000000
VERSION     DBNGSV010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1659405"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1954)
                     /locus_tag="AC159U_13305"
     CDS             complement(146..1954)
                     /locus_tag="AC159U_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338072.1"
                     /translation="MKSKYISLLFLTLAILTFYTNSSRAQYVWFDDIKEYKQLDQKKS
                     DGLSKSIYQAIKSRSKLTLDSIDLKKHDYPNIVFISASDSITPAHVGYGSANNLINAL
                     NNALDNLYNTVPKDFDLKWLKVDIVKDYNRIDNFSFRKPITIDHTLYGIAFNQASGIA
                     LIPEEVNSAVIITNGVINKYVLASYLHGKDHRYLDYSSLDYWGNNTLYSFSTDSYFYD
                     GNIGYKLFRGHRADLELDTETLHTSARLAGKYLLNAIQPDGSYIYRYHPEADLSANDY
                     NILRHAGSTYSLLELFEHSGNESVLEKAKLAIDYLKTFTRECRLEGTPYLCVIDEQPG
                     HLVKLGGNGLASAVLAKYIQLTEDKSELEYLNGVNSYILNAIKEDGSFYPHIVEFFTG
                     NVSESTSDYYPGEAILGLLLSYELTNDKKLLDGAIKASDYLIKVRDKGNTVDNINHDH
                     WLVYALSRLYKYKPDELYLNHIFKITDAIVKSQNNDSKISDINGSYYDIPRSTPAATR
                     SEALFTAYNIAKETGNNKKADIYLSTLKKTVLFQLYNQYMPESSMYMADPQQTIGGFR
                     GGYYNSEIQNDYIQHNLSSILGLLKIMEQDKQKSRN"
     gene            2070..2447
                     /locus_tag="AC159U_13310"
     CDS             2070..2447
                     /locus_tag="AC159U_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338073.1"
                     /translation="MLTLLLSLGIRSLITDDNRYGWGTFSKQANYVIKYSYIKKDGST
                     HEYKPGNELIGLANQIRHIGSTRYSTGFIKHSVENYLKYLYRNKNDEEIKTFRAELYY
                     VINKNFNHRFSKEQATLIELSYP"
     gene            2460..3632
                     /locus_tag="AC159U_13315"
     CDS             2460..3632
                     /locus_tag="AC159U_13315"
                     /inference="COORDINATES: protein motif:HMM:NF016946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HIR3338074.1"
                     /translation="MNNNCLLSAWNKYWFEEKSRESFCLIRIFFGLVFLVKLTFFIPL
                     LREGVVNIIIPKFTLYDTGLFHIEGFRNAVPGFEWLPVPSFTQYQNIEIIIIILAVLF
                     TIGLFTRYVGPVLAIIYTYLFLISQHNYHHHVFLIVIVLYILGFSNCYMHYSMDSYLG
                     LNKEKDGNIKYLPIRLLQVLISIVYLFSFLHKCNHGWLTGELTYLYYQQGTVSGYLVN
                     LIDSIFSVGFLSDYKIHFWKLLGPLTVITEGFLAFAIWIPRLRRFAIFVGLIFHSMIF
                     MFINVETFSFQMWVLYMAFIHTESKQNKVYYNGNNVCDLRFIRICKLLDWFKRIELIN
                     SNNKNIYAVGLDNKQMTGVVSSYLFVFSLFPITFIPAYIVNIFYSVFNRRKMVNVK"
     gene            3622..4917
                     /locus_tag="AC159U_13320"
     CDS             3622..4917
                     /locus_tag="AC159U_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338075.1"
                     /translation="MLNKVIIKFAHIILIITVFSSIGSVAQQQNSQYKNLSIEEYKLW
                     MDDYFNKIPWPEFEFDKMLTKDVLKKSYKLGNQYIINNQRPEGNFNYEFDFVSGEVPE
                     TDNQVRQAGAIWALALMLGYEQDDDTKKALDKGLEYFFKHTIEGARKDTLVISYPGDV
                     VSYTNTVGLISLAIIDYFILEKEGKIKLSTDYKDKLNKYLDGYLRHLKSIRLPSKHFS
                     NYYDINSKKSSDTFDPYADGETILAFIKAAKYLGHKELIPVIEESAIIMAKHYTYDQW
                     VEDYDSPKTKGFFQWSCMAFWEYQDVGWKDSEFYRKYVVSLAWWMINVHKTLYRQANT
                     GYVYEGVIPALLIARDMKRKNISTNLEYAIDTALYKLTTWQVGGPIKAENFFLLNNPP
                     KNINAIGGIMNFKNNPLLRIDVTQHQMHAAIMALRHVYK"
     gene            complement(4918..5754)
                     /locus_tag="AC159U_13325"
     CDS             complement(4918..5754)
                     /locus_tag="AC159U_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HIR3338076.1"
                     /translation="MENYKSLITEKFNKFLFSEINLFYVGIFRILLAIIILIVFFYVN
                     ASSQILYTNFYKYFEIYDTYIITNAYLCLAAIPVILFGLGIKPRLFGFISVVLLFPLI
                     FQYGLHISRQMLLATLLFFSFIKSDQVLSIKSILNKNINITSQPIWPVRLIQIQLSVL
                     YLINAAFKSTPEFISGEVLKGLSIMLPNFKVDLSSGFLDLGFITIPVAMLGIATVVSE
                     YYLVVGFWVKKLRIFTAIFGIVFHIILKQVVDIGFLDYVAVFLYLAFLFPWDSKYINK
                     NI"
     gene            complement(5726..6022)
                     /locus_tag="AC159U_13330"
     CDS             complement(5726..6022)
                     /locus_tag="AC159U_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338077.1"
                     /translation="MADIKLLRINPDNTVVNEEIGNIPKLFGYGDYKENIFWRSLENS
                     INNNVELIYSSRNYEPGTELVFIINYSYNSTKEDKTKEYRYKVATNGKLQKFDN"
     gene            6196..6672
                     /locus_tag="AC159U_13335"
     CDS             6196..6672
                     /locus_tag="AC159U_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338078.1"
                     /translation="MKTILFSVFVVLCMIIVSFSTSEAGILSPVNLGEGDGCSALSDP
                     DIDCSDLDISVLGPQDFCGTMGRKGIVVRSNGSNDTDSDNESDGIEECICDAIDDNPN
                     ININPGDGSICDSSIDAERDQFNGTLSLGSGIQGQKYEFCYTFTSFSSSICSQQNE"
BASE COUNT         2362 a         1061 c         1214 g         2472 t
ORIGIN      
        1 agaaaacact ttgttttgta tctaaaagag atggaattta gatttaataa cagaaatgta
       61 gatttagtta ataaaataaa ggagattctg cattacttat agctctgttc tatgcgagct
      121 cctaaaagaa ttaaaaaagc cggacttagt tccggctttt ttgtttgtcc tgttccatta
      181 tcttaagtag tcctaaaatg cttgagagat tgtgctgtat ataatcattt tgtatttccg
      241 agttataata accaccccta aaacctccta ttgtttgctg agggtcagcc atatacattg
      301 acgattcggg catatactga ttgtataatt ggaaaagaac agttttttta agtgtactga
      361 gataaatatc tgctttttta ttgttaccag tttccttggc gatattataa gcagtaaata
      421 gagcctctga tcttgttgct gctggtgtag accttggaat atcataataa ctaccattta
      481 tatctgagat tttagaatca ttattttgag attttacaat tgcatcagtt attttgaata
      541 tgtgatttag atataattca tctggtttgt acttatataa cctgcttagt gcataaacaa
      601 gccagtgatc gtgatttata ttatccacag tattaccctt gtctctaacc ttaattaaat
      661 aatccgaagc cttgatagca ccgtccagaa gttttttgtc attagttaat tcataactta
      721 ataatagacc aagtatggcc tctccaggat agtaatccga tgtgctttct gatacatttc
      781 ctgtaaagaa ttcaacgata tgagggtaga agctgccgtc ttctttaata gcattgagaa
      841 tatagctgtt tacgccgttt agatattcga gctcggattt atcttcagta agctggatat
      901 attttgctaa tacagctgaa gcaagtccat ttccccccaa ttttacaaga tggccaggct
      961 gttcatcaat tacacaaaga taaggtgtac cttctaatct gcactctctt gtaaatgttt
     1021 ttaaataatc aatagcgagt tttgcttttt caagtactga ttcgttgccg gagtgttcaa
     1081 atagttctag tagcgaatat gtagaacccg catgccgtaa aatattgtag tcgttagctg
     1141 agagatcagc ctcggggtga tatctgtata tatagcttcc atcaggctga attgcattta
     1201 gcaggtactt gcccgcaagt cttgctgatg tatgaagtgt ctctgtatct aattctaaat
     1261 ctgctcgatg tcctctaaaa agcttgtagc caatattccc atcataaaaa taactgtcag
     1321 tagaaaagct ataaagagtg ttatttcccc agtaatccaa actgctatag tcaagatatc
     1381 tgtggtcttt tccatgaaga taacttgcaa ggacatactt atttatgacc ccgtttgtta
     1441 taatcactgc agagttaact tcctctggaa ttagcgctat gcctgaggct tgattaaatg
     1501 ctattccata taaagtatga tctattgtta ttggttttct aaagctaaaa ttatcaatcc
     1561 tattataatc tttaactata tcaactttga gccattttaa atcaaagtct ttcggcacag
     1621 tattatatag attatcaagt gcgttattta gagcatttat aagattattt gcagaaccat
     1681 atcctacatg agcaggagta attgaatcac tggcggatat aaatacgata ttcggataat
     1741 catgtttttt aagatcaata ctgtccagag ttagctttga tcttgatttt atggcctgat
     1801 agattgattt ggatagtccg tcggattttt tctgatcaag ctgcttatat tctttaatgt
     1861 catcgaacca tacatattgc gcacgagacg aatttgtata aaaggttaat atcgctagag
     1921 ttagaaatag taatgatata tatttagatt tcatacttgc tccttgtaaa attaaatctg
     1981 tttaatatat acaaatatac gagatgaaca aaaactcttg ccaaaaatac agtgatataa
     2041 aatggtattg gaatatacca attgtaatta tgttaacgct attattatct cttggaatta
     2101 gatcactaat aacagatgat aatagatatg gatggggaac tttttcaaaa caagctaatt
     2161 atgtaataaa gtatagttat attaagaaag acggttcaac tcatgaatat aaacctggta
     2221 atgaactcat aggcttagca aatcaaatca gacacattgg ttctacaaga tattccactg
     2281 gttttataaa acattcagtt gaaaattatt tgaaatatct atacaggaat aaaaatgatg
     2341 aagagataaa gacttttaga gcagagttgt actatgttat taacaagaac tttaatcaca
     2401 ggttttccaa ggaacaggca actcttatag aattatcata tccgtaatat ttgaacccaa
     2461 tgaataataa ttgcctatta tctgcatgga ataaatattg gtttgaagaa aaatcaagag
     2521 agagtttttg tcttataaga atatttttcg gattggtatt tcttgtaaaa ttaacatttt
     2581 ttattccact gcttagagaa ggagtggtaa atattattat tccaaaattt acgctttatg
     2641 ataccggatt atttcatatt gagggattca gaaatgcggt acccggtttt gagtggcttc
     2701 ctgtaccttc gtttacgcag tatcaaaata ttgaaataat tataatcatt ctggcagtac
     2761 tatttactat tgggctattt acaagatatg ttgggccagt attagcgatt atttatacct
     2821 atttgttttt aatcagccag cataactacc accaccatgt attccttatt gtgatagttt
     2881 tatacattct tgggttttca aattgttata tgcactacag tatggattct tatctgggat
     2941 tgaataaaga aaaggatggt aatataaaat atctgccgat aagactactt caggtcctaa
     3001 tcagcattgt atatctgttc agtttcctgc ataagtgcaa tcatggctgg ttaaccggtg
     3061 agcttactta tttatattat cagcagggta ctgttagcgg ttatttggtt aatttaatag
     3121 attctatctt ttcggtcgga tttctttcag attataaaat acacttttgg aaattactag
     3181 gccctttaac tgttattact gaagggtttc ttgcatttgc tatatggatt ccaagactca
     3241 gaagatttgc tatctttgtg ggtctgatat ttcactctat gattttcatg tttataaatg
     3301 tagaaacatt cagctttcag atgtgggtat tgtatatggc gtttatacat acggagtcaa
     3361 aacaaaataa ggtttattat aatggaaata atgtttgtga tttaagattt ataagaattt
     3421 gtaagttgtt ggattggttt aaaagaatag agttgataaa cagtaacaat aaaaatattt
     3481 atgcagtggg attggataat aaacaaatga caggagttgt tagttcatat ttatttgttt
     3541 ttagtctgtt tcctataaca ttcatacctg cttatatagt aaatattttc tattcagtat
     3601 ttaataggag aaaaatggtt aatgttaaat aaagtaatta taaagtttgc tcatattatt
     3661 ctaatcataa cagtattttc atctatcggt tctgttgcac agcaacaaaa tagtcaatat
     3721 aagaatctca gcattgaaga atataaactt tggatggatg actattttaa taaaatacca
     3781 tggcctgaat ttgagtttga taaaatgctt acaaaagatg tacttaagaa atcatacaag
     3841 cttggcaatc aatacataat caacaatcaa agacccgagg gtaactttaa ctatgaattt
     3901 gattttgttt ccggcgaggt gccagaaaca gataatcagg tacggcaggc tggggctatc
     3961 tgggctcttg ctttgatgct tggatatgaa caggatgatg atacaaaaaa ggcgcttgat
     4021 aaaggtctgg aatatttctt taagcatact atagaagggg cgagaaaaga tacactggtt
     4081 atatcttatc ccggggatgt agtatcgtac acaaatacag ttggtcttat atcacttgct
     4141 ataattgatt actttatttt agaaaaagaa ggaaaaataa aactaagcac agattataaa
     4201 gataaactta ataaatatct tgacggttat ttaaggcatc ttaagtcaat acgcttacct
     4261 agcaaacatt tctcaaacta ttatgatatt aacagtaaaa aaagttcaga cacattcgat
     4321 ccatacgcag acggcgaaac tattcttgcc tttataaagg ctgcaaaata tctcggtcat
     4381 aaagaactga ttccagttat agaagaatct gcaatcatca tggccaaaca ttatacatat
     4441 gatcagtggg tcgaggatta tgattcaccc aaaacaaagg ggttttttca gtggagttgt
     4501 atggcatttt gggaatatca ggatgtaggg tggaaagatt cggaatttta tagaaaatat
     4561 gtagtatcgc ttgcatggtg gatgatcaat gttcataaaa ctttatacag gcaggcaaat
     4621 acgggttatg tgtatgaagg ggtgataccg gcgctgctta ttgcaagaga tatgaaaaga
     4681 aaaaatattt ctactaacct ggaatatgct atagatactg ctttatataa gcttacaacc
     4741 tggcaggttg gcgggcctat taaagctgaa aacttttttc tcctgaataa tcctccaaag
     4801 aacattaatg ccattggcgg cattatgaat tttaagaata acccactact aagaatagat
     4861 gtaacccagc atcagatgca tgctgctatt atggcattaa gacacgtcta taaatagtta
     4921 gatgttttta ttgatatatt tactatccca tgggaatagg aaagctaaat aaaggaatac
     4981 tgctacataa tccagaaaac caatatccac tacctgcttc aatattatat gaaatactat
     5041 tccaaatatt gctgtaaata ttcttaactt tttaacccaa aatcctacta ctagatagta
     5101 ttctgaaaca acagtagcta taccaagcat agctaccgga atagttataa aacctagatc
     5161 taaaaatcca cttgatagat cgaccttgaa attaggaagc attattgata aacctttaag
     5221 tacttcaccg ctgataaatt ctggagtaga tttaaatgct gcatttatta agtataaaac
     5281 tgatagctgt atttgtatta gtcttacggg ccatataggt tgagaagtaa tatttatatt
     5341 tttatttaga atgcttttga tacttagaac ctgatcgctt tttataaaag aaaagaataa
     5401 aagcgttgca agtaacattt gtctgcttat atgtaatcca tactgaaaaa ttaatgggaa
     5461 gagtaaaaca actgagataa aaccgaataa tctgggttta atacccaggc caaaaagaat
     5521 aacagggatt gctgccaggc atagatatgc atttgtaatg atataggtat catagatttc
     5581 aaaatatttg tagaaatttg tgtagagtat ctgtgaggag gcatttacat aaaaaaagac
     5641 tataagaatt attattgcaa gaagtattct gaatattcct acataaaaaa ggttaatctc
     5701 actaaataga aatttgttaa atttttcagt tatcaaactt ttgtaatttt ccattagttg
     5761 ctactttgta tctgtactct tttgttttat cttcttttgt agagttatat gaatagttga
     5821 taataaaaac taactctgtt cccggctcat aatttctgga gctataaatt aattccacat
     5881 tgttgtttat agagttttca agacttctcc agaaaatatt ttctttgtaa tctccataac
     5941 caaacagttt aggaatattg ccgatttctt catttactac agtattgtcc ggatttattc
     6001 taagaagttt tatatcagcc acatatacat catggtatac agctagcctg taagagccta
     6061 atattataag aataaggaat gtgataaaga ttattttatc taatctttga gaaattattg
     6121 aaatattgcg cattaatttg atttattaat actaaagtat aaaactactt aattacataa
     6181 tagaggagat ggaatatgaa aacaatatta ttttctgtat ttgtagtact ttgcatgatt
     6241 atagtttcct tttctacctc agaagcaggc attctttcac cagttaatct tggagaagga
     6301 gatggttgct ctgcactttc agatccagat attgactgtt ctgatcttga tatatctgtt
     6361 ttaggtcctc aggatttttg tggcactatg ggcaggaaag gtattgtagt tagatccaac
     6421 ggatcaaatg atacagattc agataatgaa tcagatggta tagaagaatg tatatgtgat
     6481 gcaattgatg ataatccaaa tatcaatatt aaccctggtg acggaagtat ttgtgactct
     6541 tcaatagatg ctgaaagaga ccagtttaat ggcactttga gcttaggttc gggaatacag
     6601 ggccagaaat atgagttttg ttacacattt acaagttttt caagttctat ttgttctcag
     6661 cagaacgaat gaaaattaaa tatataaagg aaaggcaagt tcattacctt tcctttataa
     6721 aatgacctga tatatccaac aaaaataaat aatcagaata ctagttattt aagattatct
     6781 atattcagct tctttctagc ttattcctat ctgttcgata tttactgggt agtatcgtct
     6841 tttcctgata taaaggcatg ggagtatgaa tacatactcg acacagttgt tttcatatcg
     6901 attgtaatag ctttattaaa accttcttca ctaccaagaa tattagtagc tttactattt
     6961 atatttcttg atattataga tcggctgcct ctttcaccaa accatattgt attccttgca
     7021 tttataatac tctcattctt tatatgtcta ttagtatcat atattaggtc taaaaaagga
     7081 tttagtattc attcatttat aaacaattt
//
LOCUS       DBNGSV010000374        10206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1382237, whole genome shotgun sequence.
ACCESSION   DBNGSV010000374 DBNGSV010000000
VERSION     DBNGSV010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1382237"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /gene="asnB"
                     /locus_tag="AC159U_13340"
     CDS             <1..1776
                     /gene="asnB"
                     /locus_tag="AC159U_13340"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3338079.1"
                     /translation="DVSSLGRQPMSNDKGTVWVTYNGELYNFKEIRQELIQQGYSFKS
                     NSDTEVLIKSYEQWGIDCIHKFIGMFAIALWDSREKKLFLIRDRAGVKPLYYYHNNGL
                     LLFASELKALHEHPKFPKEIDYRVLPTYLKYGFISNPRTIFKDTNCVRPGHYVCFEDH
                     TINEVKYWDILDHYLKDKLTHSEYEITEQLEEMLIDSFKYRMVSDVPVGVFLSGGVDS
                     SIVTALLQKNMSSKLKTFSIGFYEDKYNEACHAKRIADYLGTDHTEYYVSGKEALDVV
                     DRLAEIYDEPFGDNSGIPTYLVSKLARQDVTVALSADGGDEMFFGYNRYNKAVYYYDH
                     INRYPSAIKNLIQKTISGISPDSVEYLYGHLKFLLPEVRGVFDKYSSFQAMIEQGVKR
                     NLKNIHKITHAKWRDNELDELIIDPYSIPFTDEVFDESFCRLSEDDYMNQMLAADFKT
                     YMCDDILTKVDRASMSVSLESREPLLDHRLLEFAARIPSHLKHKDGQSKYILKKVLEK
                     YIPRDLWDRPKRGFTSPVLKWLTTDLKPLVQHYLGEEKIKQDGIFCPKTTKSWVDKLY
                     NGKSVKAERIWYMLMFQIWKERWMH"
     gene            complement(1801..3006)
                     /locus_tag="AC159U_13345"
     CDS             complement(1801..3006)
                     /locus_tag="AC159U_13345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3338080.1"
                     /translation="MKILIYTHEYPPFLGGLATTSYKLIRGLSKKDDLEIFILAPSYG
                     AGDESVDKEHGEKIVRVPILRSKIAKFIPILYYLISSIFFYISLKKIKPDLVLFITEE
                     AEATGGFVSYFCDFTSIARVAGSGITTCFLGNKPFKKLLKYPIARLYKISKKIIAVSN
                     YSKDLLLQVGVDGEKITVINNGISEQLISKSTDNKKLNKIKKTYSIKTDEKILVTIAR
                     ILPRKGQDNVIRALKLAEDELGDFRYLIVGTGRYETQFKELARSLGLEDKVIFTGGVD
                     HDEIIHCLDLCDLFIHANRFWNNKVEGLPNAVLEASARAKPVIVGNDSGSIESVIDGK
                     TGFLVDSESPEQIAQAILKILSDPGLAQTMGKNGKQMIVQRFTEEEMIENYYNLFKSL
                     YNSDDNPVA"
     gene            3113..3355
                     /locus_tag="AC159U_13350"
     CDS             3113..3355
                     /locus_tag="AC159U_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338081.1"
                     /translation="MSDIQTSLLKSMCTGLGEGNSNIEALASKIKEDFPEKDKAELKG
                     EILDELKGMVSSGQLQIITTGWEFGNEFFYICSKKL"
     gene            3401..4279
                     /gene="xerC"
                     /locus_tag="AC159U_13355"
     CDS             3401..4279
                     /gene="xerC"
                     /locus_tag="AC159U_13355"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR3338082.1"
                     /translation="MTGYINQFKNYLDSERNYSAHTLRAYGKDLDDFARFLQSKNIAS
                     DLNEINDSHIRMYMGLLNTKNSKSTIERKLASLRAFFDYLQKEKIIKSNPAKLIPTPK
                     KEKKLPVFLSVDEMFRLIDQSVYKGVRGIRDKAMLELMYSSGIRVGELVNLKLDSLNI
                     DKNELKLLGKGSKQRIVPVGKKAIESLLEYLQKRELLKPKTDNVFINNRGDKISERSI
                     GRIVKKFAQAASLSKNISPHALRHSFATHLLGSGADLRAIQDMLGHSSLSVTQKYTHT
                     SIERIMEVYDKTHPRS"
     gene            4307..4840
                     /gene="hslV"
                     /locus_tag="AC159U_13360"
     CDS             4307..4840
                     /gene="hslV"
                     /locus_tag="AC159U_13360"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR3338083.1"
                     /translation="MKDLRSTTIVCVKKGEKIAIAGDGQVTLGATALKHTAKKLRRIY
                     NDSVVVGFAGSTADAITLFDKFEAKLQEFRGNLRRSAVELGKDWRTDRILRRLEALLI
                     VADKESMFLISGSGDIIEPDDDVIAIGSGGPFAQAAAKALMRHSDLEIKEIVKQSLLI
                     ASEICIYTNDNIVIEEL"
     gene            4845..6188
                     /gene="hslU"
                     /locus_tag="AC159U_13365"
     CDS             4845..6188
                     /gene="hslU"
                     /locus_tag="AC159U_13365"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR3338084.1"
                     /translation="MDKAENNLITLTPRETVTELDKFIVGQKEAKKAVSIALRNRWRR
                     QRVSAEMRDEIAPKNILMIGPTGVGKTEIARRLARLSQAPFLKVEASKFTEVGYVGRD
                     VESMIRDLADIAVKMITEEEKKNVISKAKELAEERILDILLPKTGDAEPEAESQSETR
                     QKMRKMLREGKLDDRSIEIEVTAKNFPLQIFSQSGIEEMDVNISDMLGNLMPKKSKRK
                     KVKVPDALEALVQEEAQKLVDMDKVVKQAVERVEQSGIVFVDEIDKICGREASSGPDV
                     SREGVQRDLLPIIEGCTVNTKHGLVKTDHILFVGAGAFHVSKPSDLIPELQGRFPIRV
                     ELDALSKDDFVRILTEPKNALIKQYKELLKTENISLEFTDDAIIRLAELAEEVNETTE
                     NIGARRLHTILEKILEEISFDAPDIHDTEFRVDSEYIKNKISDIVKDRDLSRYIL"
     gene            6216..7097
                     /gene="argB"
                     /locus_tag="AC159U_13370"
     CDS             6216..7097
                     /gene="argB"
                     /locus_tag="AC159U_13370"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3338085.1"
                     /translation="MNELINKAKTLSEALPYIREFYGKTIVIKYGGSINSHELNSFAR
                     DVVLMKYVGMNPVVIHGGGPQIGDYLSKLGIDTEFIAGLRVTTDIVMEVAQMVLVGKV
                     NQDIITAINLLGGTAVGVSGKEGKLITAEKLDINKFAKENGIKIPAKADVGHVGEVVY
                     INPRLIETLDNSGYIPVIAPIGFDEQGNSYNINADHVAGKVAGALNAEKFIILTNVPG
                     ILNKKKELISTLHEKEVKKLIRSNVISKGMIPKVICAINALNEGVKKVHMIDGTKEHS
                     LLLEVFTDKGIGTQILI"
     gene            7094..8299
                     /locus_tag="AC159U_13375"
     CDS             7094..8299
                     /locus_tag="AC159U_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3338086.1"
                     /translation="MKSSDVIAALDSYGFKNYGRYPIALQKAKGCWVWDFEGNKYLDF
                     TTGIAVNNLGHNHPKVKKAISSQLNKIVHSSNLFYTEEQGKLARLLVENSFADRVFFC
                     NSGAEANEAAIKLARKWGNENGGRYKIVSTKGGFHGRSYGSLSITGHKKYRQGFNPMV
                     PGVKFVDYGDFSQLQKAAGDKKVCAVILEPIQGENGVIIPPNGYLKKVRKLCNQNNIL
                     FILDEIQVGVGRTGSMFAYEQLGFKPDLMTLAKALGGGLACGALLAKNKHAKYLTPGS
                     HGSTMGGNPLAMSVGYSVISTIMDDGLLDNVQTLGSYFISKLKEVQSHNSNIIKEVRG
                     SGFIIGIELANNKYASEVVKKLYKRGLLTIITEQKNIRILPPLIAGKKEIDIALGIIR
                     TVLEEVSNA"
     gene            8292..9200
                     /gene="argF"
                     /locus_tag="AC159U_13380"
     CDS             8292..9200
                     /gene="argF"
                     /locus_tag="AC159U_13380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3338087.1"
                     /translation="MPRHFLSIFDLRKEEFELLFERTKELKSSRISRDFPQSLINKSV
                     GLIFEKQSTRTRISFEVAINELGGDVIFMSSSDLQLNRGESIEDTAKVLTSYLSGIII
                     RTFDQSMIEEFAEASDIPVINALTDLEHPCQIVSDLYTLKENGVDINNMKFVYLGDGN
                     NITNSLIGAACILGFELVISTPKGYEPDKDMLRIADTYDPRIELIRDPVQAVKDADVV
                     YTDVWVSMGQEDQKYKKLEMFKDYQLNSNLLSNAKNSTLIMHCLPAHRGQEITDEIIH
                     DKNSVIYQQAENKLHAGKAILEMFIA"
     gene            complement(9190..>10206)
                     /locus_tag="AC159U_13385"
     CDS             complement(9190..>10206)
                     /locus_tag="AC159U_13385"
                     /inference="COORDINATES: protein motif:HMM:NF020048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3338088.1"
                     /translation="YYRNGSVMDKSEFFFENSFGDKDQILEEFIYRYYSDRNNYPKEI
                     CIGIELENSSFITQWLSERSGRTVKLINPKRGKKVDLINLAKENARACYIKNYHEQSN
                     FDKLFENLAKKLNLKNLPRTIECYDISNIQGTNPVASSVRFEDGKAQKKRYKKYRIKT
                     VSGPDDFAMMNEVISRRLQRKDEKGWDLPDLILIDGGIGQLNSALSAAKHNDSLEEVD
                     IVAIAKSRTRDEADKIVIPNRSNAVTFKKDSKEILYLMRIRDEAHRFAVEYHKNLRKK
                     GFIKSAFESIPMVGAKRKKILQKKYRTINNLKKVSLDELLSIEGFNTKIAKSVMDYVA
                     EAKQ"
BASE COUNT         3272 a         1816 c         2160 g         2958 t
ORIGIN      
        1 gatgtttctt ctcttggcag acagcccatg tctaatgata aaggcacagt atgggttaca
       61 tacaacggag agctgtataa ctttaaggaa ataaggcagg agcttatcca gcagggctac
      121 agttttaaga gcaattccga taccgaggtt cttataaagt cctatgaaca gtggggaatt
      181 gactgtatac acaagtttat aggaatgttt gcaattgccc tgtgggactc aagagaaaag
      241 aaactctttc tcattcgtga cagagccgga gtaaaacccc tttattacta tcataacaac
      301 ggtctgttac tatttgcttc agaactaaaa gcgcttcatg aacatcctaa atttccaaaa
      361 gaaatagatt atcgtgttct gccgacatat ttaaaatatg ggtttatatc aaacccaagg
      421 actattttta aggatactaa ctgcgtaaga ccaggtcatt atgtttgttt tgaagatcat
      481 acaattaatg aagttaaata ctgggatatt ttagatcact atttaaagga taagcttaca
      541 cactccgagt atgaaattac agaacagctt gaagagatgc ttatcgactc attcaaatac
      601 aggatggtat cggatgttcc ggtgggtgtg tttttaagcg gtggggtaga ttcgagcata
      661 gtaactgccc ttcttcaaaa aaacatgagt tctaagctaa aaaccttctc aatagggttt
      721 tatgaagata aatacaatga agcttgtcat gcaaagagga ttgcagatta tcttggcaca
      781 gaccacacgg agtactatgt gtccggtaag gaagcactgg atgtagtaga caggcttgca
      841 gaaatatatg acgagccgtt tggagacaat tctggtatcc ctacatatct tgtatcaaag
      901 cttgcaaggc aggatgtcac ggtggccctt tctgcagatg gcggtgacga gatgtttttt
      961 ggttataaca gatacaacaa ggcagtgtac tattatgacc atataaaccg ttacccctct
     1021 gcgatcaaga atctgataca aaaaacaata tccggcataa gtccagatag cgtggagtat
     1081 ctatacggcc accttaaatt tttgctgcca gaagttagag gagtgtttga taaatactca
     1141 agttttcagg cgatgataga gcagggagta aaaagaaatc tcaaaaatat acataaaata
     1201 actcatgcca aatggagaga taatgagtta gatgaactta taatcgaccc atattccatt
     1261 ccttttacag atgaagtgtt tgatgaaagc ttttgccgct taagcgaaga tgattatatg
     1321 aatcagatgc ttgctgcgga ttttaaaacc tatatgtgtg atgacatact cacaaaagtg
     1381 gacagggctt ctatgagcgt gagtcttgag agcagagagc cgcttcttga ccacaggctg
     1441 ctcgaatttg cagcccgtat accgtcacat ctaaaacaca aggatggaca gtctaaatat
     1501 attttaaaaa aagtgctgga aaaatatatt cctcgcgacc tgtgggacag gccaaaaaga
     1561 ggttttactt cccctgtgct taaatggctt acaactgatc ttaagcctct tgtacaacat
     1621 tatctgggtg aagaaaaaat aaagcaggat ggcatattct gtcctaaaac aacaaagtcc
     1681 tgggtggata aactatataa tggtaaaagt gtaaaggcag aaaggatctg gtatatgctg
     1741 atgtttcaaa tatggaaaga aaggtggatg cactaatact ctccagggtc tccagggggc
     1801 ttaagctaca gggttgtcat ctgagttata taaggactta aagagattat agtaattttc
     1861 aatcatttct tcttcagtaa atctctgtac aatcatctgt tttccatttt tgcccattgt
     1921 ttgtgcaagt ccgggatcag aaagaatttt taaaatagcc tgcgctattt gttcgggact
     1981 ctctgaatct accaagaatc cggttttacc gtctataaca gattcaatag agccgctgtc
     2041 atttccaact ataacgggtt ttgccctcgc gcttgcttca agcaccgcgt ttggaagtcc
     2101 ctcgaccttg ttgttccaaa acctgttggc atgaataaag aggtcgcaaa ggtccagaca
     2161 gtgtatgatt tcgtcgtgat cgacgcctcc ggtgaaaata actttatctt ccagccccaa
     2221 tgacctggca agttctttaa actgagtttc atacctgcct gtgcctacta ttagatatct
     2281 aaagtcacca agctcatcct ctgccaattt aagcgccctt attacattgt cctgcccttt
     2341 tctaggtaga attcttgcaa tcgtaaccaa tattttttca tcagttttta ttgaatacgt
     2401 tttttttatt ttattaagtt ttttattatc tgtagattta gaaattagtt gttcacttat
     2461 tccattgttt attactgtta ttttctctcc atctactcca acttgtaaaa gcaggtcctt
     2521 tgaataattg cttacggcta taattttttt agatatttta tataacctgg caattggata
     2581 ttttagtaat tttttaaatg gtttattgcc tagaaaacag gttgttattc cagaacctgc
     2641 tacccttgca atagaagtga aatcacaaaa atagcttaca aagccaccgg ttgcttctgc
     2701 ttcttccgtt ataaacaaga cgaggtctgg ttttattttt ttcaaagaaa tataaaaaaa
     2761 gatactcgat attaaatagt ataaaatagg aataaatttt gcgatctttg aacgtaatat
     2821 tggaactctt actattttct caccatgctc tttatcaaca ctttcatctc cggctccata
     2881 ggaaggtgct aatataaata tttccagatc atctttcttg gacagacctc ttataagttt
     2941 gtaactggtg gttgcaagcc cgccaagaaa cggcgggtat tcgtgagtat agattagaat
     3001 cttcattgct taaggcctgt tggatagaaa taatccaata tatttgttat agtattctgt
     3061 tttttaatgg atttactata tttggataaa aatatattag ttaggagatt ttatgtcaga
     3121 tattcaaact agtttattaa agtcaatgtg tacaggactt ggagagggca actctaatat
     3181 tgaagctctt gcttcgaaga taaaagaaga ttttcctgaa aaagacaagg ctgaactaaa
     3241 aggagaaata ctcgatgagc ttaaagggat ggtctcatca ggacagcttc aaattattac
     3301 aacaggctgg gaatttggca atgaattctt ctatatttgc tccaaaaaac tctaagccct
     3361 tgaacaacta agcataatgc ttattgtata acctttaaaa atgacaggct atattaatca
     3421 atttaagaat tatttggact ctgaacgaaa ttactctgcc cataccttaa gagcctatgg
     3481 taaggacctg gacgactttg cccggttttt gcagtctaag aatatagcat ctgatctaaa
     3541 tgaaattaat gattcacata ttcgcatgta tatgggactt ctaaacacga aaaactctaa
     3601 atctaccatt gaaaggaagc ttgcctcact tcgtgctttc tttgattatc tgcaaaaaga
     3661 aaaaattatt aagtcaaatc cggcaaagct cattcctaca ccaaaaaaag agaagaaact
     3721 cccggtattt ttatcagtcg atgaaatgtt caggctcata gaccagagtg tttataaagg
     3781 agtgcggggc ataagggata aagccatgct cgaactcatg tattcaagcg gtattcgcgt
     3841 tggtgagctt gtaaatttaa aactagatag tttgaatatt gataaaaatg aactcaagct
     3901 tcttggtaaa ggcagcaagc agcgaatcgt tcccgtagga aaaaaggcta tcgaatcgct
     3961 tttggaatat ttgcagaaaa gagagctgtt aaaaccaaag acagataatg tttttataaa
     4021 taacagaggt gacaaaatat ccgaaagaag cataggacgg attgttaaaa agtttgctca
     4081 ggctgcatca ctcagcaaga atatcagccc ccatgcgctc aggcactcat ttgccacgca
     4141 tcttcttgga agcggcgctg atctaagagc aatacaggat atgctcgggc actcaagcct
     4201 ttctgttaca caaaaatata cacacacttc catagaacgt atcatggaag tttacgataa
     4261 aacccatccc agatcataga agttttaagt taagaggatt ttatatatga aagatttaag
     4321 aagcactaca attgtttgtg ttaagaaagg agaaaaaata gccattgcag gagacggaca
     4381 ggttacgctc ggggcaactg ctctaaagca cacggcaaag aagcttagaa gaatttacaa
     4441 tgactcggtt gtcgtgggat ttgccggatc cacggctgat gcaattactc tttttgataa
     4501 atttgaggca aagcttcagg aattcagggg aaacctcaga agatcggcag tggagcttgg
     4561 aaaggactgg agaacggaca gaatcctgcg caggctcgaa gcgcttttaa ttgtagcaga
     4621 taaagaatcg atgtttttaa tatcgggaag cggggatatt atagagcctg atgacgatgt
     4681 gattgccata ggttccggag gtccttttgc acaggctgct gcaaaggctc ttatgagaca
     4741 ttctgatctg gagattaagg agatagtaaa acagtcgcta ttaattgctt cagaaatatg
     4801 tatttataca aacgataata tagttataga ggagttatag attagtggat aaagctgaaa
     4861 ataatttaat cactcttaca ccaagagaaa ccgtaacaga gctggataag tttatagttg
     4921 gtcaaaaaga ggccaaaaaa gcagtctcaa ttgcccttag gaacaggtgg agaaggcaga
     4981 gggtgtcagc tgagatgaga gatgagattg cgccaaaaaa cattcttatg attggtccaa
     5041 caggagttgg taagactgaa attgcaagaa ggcttgcaag gctttcacag gcgccgtttt
     5101 taaaagtgga ggcttcaaag tttacagagg ttggctatgt aggaagagat gtggagtcca
     5161 tgataaggga ccttgcggat attgctgtaa aaatgataac cgaagaagaa aagaaaaatg
     5221 tgatcagcaa agctaaggaa cttgcagaag aaaggatcct tgacatattg cttccaaaga
     5281 caggcgatgc tgagcctgag gccgaatcac agagcgagac cagacaaaag atgagaaaga
     5341 tgttaaggga agggaagctt gacgacaggt caatcgagat tgaagtcact gccaaaaact
     5401 ttccgcttca gattttctca cagtcgggca ttgaagagat ggatgtaaat atttcggata
     5461 tgctgggaaa tcttatgccc aaaaagtcca agagaaaaaa ggtcaaagtt cccgatgcat
     5521 tagaagcatt ggttcaggaa gaggcccaga agcttgtaga tatggataag gttgtaaagc
     5581 aggccgtgga aagggtggag cagtcgggaa tagtctttgt ggacgaaata gataagatat
     5641 gcgggaggga agcttcgtcc ggccctgatg tttcacgcga aggagtacag agggatctgc
     5701 ttcctataat tgagggctgt actgtaaaca ccaaacatgg gctagtaaaa accgaccaca
     5761 ttctttttgt tggagcaggg gcatttcatg tctctaagcc gtctgacctc ataccggagc
     5821 ttcagggaag gtttccaatc agggtagagc ttgatgcact atctaaagat gattttgtaa
     5881 gaatcttaac agagcctaaa aatgcactca taaaacagta caaggagctt ttaaagactg
     5941 aaaacatttc tcttgaattt accgatgatg cgataataag gcttgccgag ctggcagaag
     6001 aggtaaacga gaccacagag aatataggtg ccagaaggct tcatacaata ctggaaaaga
     6061 ttcttgaaga aatatcattt gatgcccctg atattcatga tacagaattt cgggtagatt
     6121 cagagtatat taaaaacaaa atatcagaca tagttaagga cagggatttg agccgctata
     6181 tactctaggc ttttgaaatt tctcttacta gataaatgaa tgaattaata aacaaggcaa
     6241 agacactaag cgaggctctt ccgtatataa gggagtttta cggaaagacc attgttataa
     6301 agtacggtgg aagcataaac tctcatgaac ttaacagttt tgctcgtgat gttgtgctaa
     6361 tgaaatatgt agggatgaat cctgtagtta ttcacggcgg cggcccgcag attggagatt
     6421 acctatcaaa gcttggcatt gatactgaat ttattgcagg tctaagggtt actacggata
     6481 ttgtaatgga agtggcccaa atggtgctgg taggaaaggt aaatcaggat atcatcacag
     6541 ctattaacct tcttggcggt acggcagtgg gagtatccgg caaggaaggc aaactgataa
     6601 cagccgagaa actcgatata aataagtttg caaaagaaaa cggcataaag attccggcta
     6661 aagctgatgt aggtcatgtg ggagaggtcg tatatataaa tccgaggcta atagaaacac
     6721 tcgataattc aggctatata ccggtgatag cgccaatcgg gtttgatgaa cagggcaatt
     6781 cttacaacat aaatgccgac catgtagcag gtaaggtagc tggtgcgctt aatgctgaaa
     6841 aattcataat ccttacaaat gttcctggaa tactgaacaa gaaaaaagag cttatatcca
     6901 cactacatga aaaggaagta aaaaagctta tacgcagcaa tgtaatttca aaaggaatga
     6961 tccccaaggt catttgtgcc ataaacgcac taaacgaagg agtaaagaag gttcatatga
     7021 tagatggtac caaggaacac tcgctgctct tggaagtgtt tacagataaa gggattggaa
     7081 cacagatact gatatgaaat cttctgatgt aatagctgct ctggacagct atggatttaa
     7141 aaattacgga agatacccca tagcactgca aaaagccaaa ggatgctggg tctgggattt
     7201 cgagggcaat aaatatctgg actttactac gggtattgca gtcaataacc ttggtcataa
     7261 tcatccgaaa gttaaaaaag caatatcttc tcagttaaat aagatcgttc acagctcaaa
     7321 cctcttttat accgaagaac agggaaaact tgcaaggctt ctcgttgaaa attcatttgc
     7381 agaccgggtg tttttttgca acagcggagc cgaagcaaac gaagccgcga taaaacttgc
     7441 aaggaaatgg ggaaatgaaa acgggggcag gtataaaatc gtatcaacta aaggcggatt
     7501 tcatggaagg tcatacgggt cgcttagcat aacgggacac aagaagtaca ggcagggctt
     7561 taacccgatg gtgcctggtg tgaaatttgt agattatggt gatttctccc agcttcaaaa
     7621 agcagccggt gacaaaaaag tatgcgccgt aatactcgaa cccatacagg gtgaaaatgg
     7681 agtaataatt ccaccaaatg ggtatttgaa aaaagtccga aaactatgta atcaaaacaa
     7741 tatcttattc atattagacg agattcaggt tggtgttgga agaaccggaa gcatgtttgc
     7801 ctatgaacag cttggtttta agccagacct tatgacgctt gcaaaagcgc ttggaggcgg
     7861 gcttgcctgc ggggcgctgc ttgcaaaaaa caagcatgca aaatatctga ccccgggctc
     7921 tcatggttct acaatgggag gaaacccact tgccatgagc gttggttata gcgttataag
     7981 caccataatg gatgatggtc tgcttgataa tgtgcaaaca ctgggctctt acttcatttc
     8041 aaaactaaaa gaggtacaat cacataacag caatatcata aaagaagtga gaggcagcgg
     8101 gtttattatt ggcatagagc ttgcaaataa taaatatgca tcagaggttg ttaaaaaatt
     8161 atataaaagg gggcttctca caatcataac tgaacaaaaa aatataagaa ttctgccccc
     8221 gctaattgca ggtaaaaaag agatagatat tgcacttggt ataattagaa ctgtattgga
     8281 agaggtttca aatgcctaga cattttttaa gtatatttga cctgagaaaa gaagagtttg
     8341 aattattgtt tgagcgtaca aaagagctta agtcttcaag aatatccagg gattttccgc
     8401 aaagtcttat aaataaatct gttggcctga tttttgaaaa acagtccacg cgtacaagaa
     8461 tatcatttga ggttgctata aacgagcttg gaggagatgt catatttatg agctcatctg
     8521 atctgcagct aaaccgtggt gaatctatag aagacacggc aaaagtactg acgtcttatt
     8581 taagcggcat tattattaga acctttgatc agtcaatgat tgaagaattt gccgaggctt
     8641 cagacattcc ggttattaat gcgcttaccg atctggagca tccctgtcag attgtatctg
     8701 atctatatac tcttaaagaa aatggtgttg atataaataa tatgaagttt gtttatttgg
     8761 gtgatggaaa taatataaca aactccctta ttggtgctgc ctgtatatta ggatttgaac
     8821 ttgtaatttc tacccctaaa ggatatgagc ctgataagga tatgcttcgt attgcagaca
     8881 cttatgatcc caggatcgaa ttaattcgag atcctgtgca agccgtaaaa gatgctgatg
     8941 tggtctacac cgatgtgtgg gtcagtatgg gtcaggaaga tcaaaaatac aagaaactgg
     9001 aaatgtttaa agattatcag ctaaacagca atctattatc aaacgcaaaa aacagcacgc
     9061 ttattatgca ctgccttcct gctcacaggg gccaggagat tactgatgag ataatacatg
     9121 ataagaattc cgtaatttat cagcaggcag aaaataaact tcatgccgga aaggcaattc
     9181 tcgagatgtt tattgcttag cctccgctac ataatccatt acagactttg ctatttttgt
     9241 attaaagccc tcaatcgata aaagctcgtc taaacttacc ttcttaaggt tattaatcgt
     9301 cctgtatttt ttctgaagta tcttctttct ttttgcgcct accatcggga ttgactcaaa
     9361 tgccgattta ataaatcctt ttttgcgtaa atttttatga tattcaactg caaacctgtg
     9421 agcctcatcc cttattctca taagatataa aatttctttt gagtcctttt taaatgtcac
     9481 tgcattggat ctatttggaa ttactatttt atctgcttca tctcttgttc tggatttggc
     9541 tatagcaaca atatcaacct cttccaaaga gtcgttatgt ttggctgcac taagggctga
     9601 atttagctgt ccaatgcctc catcgattaa tatgaggtcc ggcaggtccc agcctttttc
     9661 gtcttttctt tgcagtcttc tgcttataac ctcgttcatc atggcaaaat catcgggacc
     9721 cgatacagtt tttatacggt attttttata tcttttcttc tgtgccttgc cgtcttcaaa
     9781 tctaactgac gatgccacag ggtttgtacc ctgaatgttt gagatgtcgt agcactcgat
     9841 ggttcttgga aggtttttca gatttaattt ttttgcaagg ttttcaaaga gtttatcaaa
     9901 gttgctctgt tcatgatagt tctttatata gcaggctctg gcattttcct ttgcaagatt
     9961 tatcagatca acttttttcc ctcttttggg atttatgagt ttaacagtcc taccacttct
    10021 ttctgaaagc cactgtgtaa taaaactact gttctcaagc tcgattccta tgcatatttc
    10081 cttcggatag ttattcctgt ctgagtaata tctgtatata aattcttcga gtatctggtc
    10141 tttatctcca aaactgtttt caaagaaaaa ttctgattta tccataaccg aaccgtttct
    10201 gtaata
//
LOCUS       DBNGSV010000375         2590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_691615, whole genome shotgun sequence.
ACCESSION   DBNGSV010000375 DBNGSV010000000
VERSION     DBNGSV010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_691615"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..587
                     /locus_tag="AC159U_13390"
     CDS             39..587
                     /locus_tag="AC159U_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338089.1"
                     /translation="MIKKVNEKDLMKDSLGRYRTSSLFIETPVKGNEAFWTMQDQDRE
                     VKGKVYPSLKQIYMTYYHIPYSEYEFAIDVFGSWDHWDRLSNESNKIIISMITAWRLE
                     MEIKIRANSLKDILRTSGSSDSAGLAAAKYIAERGWEKKAGRPTKAAIEKETKAQAGI
                     NKAINDDASRIGMEGETSTKLH"
     gene            599..937
                     /locus_tag="AC159U_13395"
     CDS             599..937
                     /locus_tag="AC159U_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338090.1"
                     /translation="MSFANQDKYRLPVKYAYLDATGAGNTAIVAAVTGKKIRVLAVAA
                     HALTAVTIHFESSTTQISADVAAGATGGYVRPMGQLPYGWFETAAGEALNVNLSAAVA
                     TGIDLVYIEV"
     gene            939..1205
                     /locus_tag="AC159U_13400"
     CDS             939..1205
                     /locus_tag="AC159U_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338091.1"
                     /translation="MGFWKWLTLQNDTPLSQRVQEPLPPLKDDRVLRCSTRLLEQYRD
                     GKTYTSADRRMIVAYQRVLKNYGYPAPVNAKEAKLLIKSLEKLQ"
     gene            1376..1534
                     /locus_tag="AC159U_13405"
     CDS             1376..1534
                     /locus_tag="AC159U_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338092.1"
                     /translation="MGQGSIKYDPNPLIEVLVFKDGTQKQRSIEYFPDIQIGDIWLGH
                     WNGKELNG"
     gene            1527..2087
                     /locus_tag="AC159U_13410"
     CDS             1527..2087
                     /locus_tag="AC159U_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338093.1"
                     /translation="MAKTYFLSADAATHSLGGQFTQLLDTAADTLTTASILATNGGGT
                     QSGYYEEAQDTGVTRTSNGTFGVEVNISSATANTQMSMQLHRVNSGGTIQNSSGHTAY
                     QATTSTGLLNFSHVLTTELGTFATTDRLVVELRVQNNAPHGADKGPTHDQETINAEVT
                     TPFTDPVGGVSIPVIMNYLKQQQRTI"
     gene            2087..>2590
                     /locus_tag="AC159U_13415"
     CDS             2087..>2590
                     /locus_tag="AC159U_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338094.1"
                     /translation="MFLRQSTSQVIRFGPCLDIGDGVTEETALTLAQADMRLSKDGGA
                     FAQKNAAGNATHDSDGWYSTTLDATDTATVGELILNVHQPANMLPVWQRYWVIEEAIY
                     DLLYGAAADGLVTLAAVTHTGAVIPTVTTNTDMRGTDSAALASVCTETRLSELDAATG
                     GKMANQVD"
BASE COUNT          874 a          435 c          573 g          708 t
ORIGIN      
        1 gcgaaacata tcaaatcaga aatcgaagat agaatttaat gattaagaaa gtaaatgaaa
       61 aggatttaat gaaagactca ttgggaagat atcgaacatc ttcccttttt attgagactc
      121 ccgttaaggg caatgaagca ttctggacta tgcaggatca ggatagggaa gtaaagggaa
      181 aagtatatcc ctcattaaaa caaatttata tgacttatta ccatattcct tattctgaat
      241 atgagtttgc tatagatgta tttggtagct gggatcattg ggataggcta tctaatgaaa
      301 gcaacaagat tataatttct atgattactg cttggcggtt agagatggag attaaaataa
      361 gagccaattc tcttaaagat atacttagaa cttcaggaag tagcgattct gcaggtttag
      421 cagcagctaa atacatagca gaaaggggtt gggagaagaa agcaggaaga cctaccaaag
      481 cagcaataga aaaagaaact aaagcacaag caggaattaa caaagccatt aatgatgatg
      541 catcaagaat cggcatggaa ggtgagactt ctaccaaatt acactaagag gatatagaat
      601 gagcttcgca aatcaagata aatatagact acctgttaaa tatgcatact tagatgcaac
      661 aggagcaggt aacacagcta ttgtagctgc agttacagga aagaaaataa gggtactagc
      721 cgtagcagca cacgcactaa cggcagtaac tattcacttt gaatcttcta ctacacaaat
      781 ttcggcagat gtagctgcag gagcaactgg tggttatgtt cgtccaatgg gacaattgcc
      841 atacggatgg tttgaaactg cagccggaga agcccttaat gttaatttaa gcgcagcggt
      901 agcaactggt attgatttag tttatataga ggtctgatat gggattttgg aagtggctta
      961 cacttcagaa tgatactcca ttaagccaga gagtacaaga accccttcct cctttaaagg
     1021 atgatagggt tcttcgctgt tctacaagat tattagaaca atatagagac ggaaaaacat
     1081 atacctcggc agatagacgt atgattgtag cctatcaaag agtattaaag aattatggat
     1141 atcctgcacc tgtaaatgct aaagaagcta aacttcttat taaatctttg gagaaattac
     1201 aataattgag tttattgtag agtatacaga caaaaccgtt tattctaata tggaggaacg
     1261 gcatagttta tgtcagtcag ggatacaaag cattaaagca ggtattaagg gttcttatgt
     1321 aacattcagc ggagctagta tttattggtg ttataatgag aatgactatt atgttatggg
     1381 acaaggatca ataaagtatg accctaatcc attaatagag gtgcttgtat ttaaagatgg
     1441 tactcaaaag caacgctcaa tagaatattt ccctgatata caaataggag atatatggtt
     1501 aggacattgg aatggtaagg aactaaatgg ctaaaactta ctttttaagt gctgatgccg
     1561 ctacccatag tttaggtggg caattcacac agttattaga taccgctgct gatactttaa
     1621 ctacggcatc tattcttgcg actaatggtg gtggtactca gtccggttac tatgaggaag
     1681 cgcaagatac cggagtaacc agaaccagta atggcacatt cggcgttgaa gtaaatatat
     1741 caagtgctac tgctaatact caaatgagca tgcagcttca tcgggttaac agtggcggaa
     1801 ctatacaaaa tagttcagga catacggctt atcaggcaac tacaagtaca ggattattaa
     1861 atttcagcca tgtacttaca acagaacttg gaacttttgc tacaactgat cggttggtcg
     1921 ttgagttacg ggttcagaac aatgctcctc atggtgctga taaagggccg acacacgatc
     1981 aggaaactat taatgcagaa gttacaaccc cgtttactga tccggtagga ggtgtaagta
     2041 tccctgtaat tatgaactat ttaaagcaac aacaaaggac tatttaatgt ttttaagaca
     2101 aagtacatca caggttatta gatttggccc ttgtctggat ataggcgatg gggttacaga
     2161 agaaactgct ctaactttag cacaagccga catgcgtctc tctaaagatg gaggcgcatt
     2221 tgctcaaaaa aatgctgctg gcaacgccac tcacgatagt gatgggtggt attcaactac
     2281 tttagatgct actgatactg ctacagtagg tgaacttatc cttaatgtac atcaaccagc
     2341 caatatgctt ccagtatggc agcgttattg ggttatagaa gaagctattt atgatttatt
     2401 gtatggagct gctgctgacg gattggttac attggctgct gtaacgcata caggggcagt
     2461 aattccaaca gtaactacaa acacagatat gcgtggcact gatagtgctg cattggctag
     2521 tgtatgtaca gaaacaaggt taagcgaatt agatgccgct acaggtggta aaatggctaa
     2581 ccaagtggat
//
LOCUS       DBNGSV010000376         2158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1382687, whole genome shotgun sequence.
ACCESSION   DBNGSV010000376 DBNGSV010000000
VERSION     DBNGSV010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1382687"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="AC159U_13420"
     CDS             complement(<1..681)
                     /locus_tag="AC159U_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338095.1"
                     /translation="MNDKRQVGLSLEILFTILFITAVAMLLISLIAFNVMERFALSGK
                     TEGLKSIIETFENVYYSSGDVPSGIIFLENTLETGAWGVIADGTRRTAFSTTNNRKQI
                     VVTDPQILDVMRTGETVVELKGFNLPPIKFHEALKIASPIKSANSKGVILIYQPLSSL
                     RSNILSGQQQVSIWIVLFLIIIASLGFYVLSNRIIKPVKELIFATEKIAEGRFPENMD
                     MGNVRELNQ"
     gene            complement(713..1552)
                     /locus_tag="AC159U_13425"
     CDS             complement(713..1552)
                     /locus_tag="AC159U_13425"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3338096.1"
                     /translation="MDPNTILLIFSFIFGAAVGSFLNVCIYRLPNDISLVFPSSHCTS
                     CKKPIPFYHNIPIISYLVLGGRCYNCKEKYSWDYPLVEFLAGLLSLLLFIKYGPTINF
                     VFLFIFVMSLIVITFIDLEHMIIPNVISYPGVVVGLIYSALITDYDSLRFLLDNLHYN
                     FLYILYVLDEVRIFSSVFGAFIGAGSLLLIGFAYKNVRKMDGLGLGDVKLMAMVGAFL
                     GWRSIIFVALISSLIGTIIGIGIMLRNKGDLKYAIPFGPFISFAATLYCFCGDLSLDY
                     FID"
     gene            complement(1581..>2158)
                     /locus_tag="AC159U_13430"
     CDS             complement(1581..>2158)
                     /locus_tag="AC159U_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3338097.1"
                     /translation="ISVKGSEVLSKFVGESEQAVRELFKKAKQVSPCILFFDEIDALA
                     PCRLENSLNRVSERIVSQLLTEMDGIEELRGVFIVAATNRPDMIDPALLRSGRFDAFI
                     QIPVPNDKALYEILKVHTKGKPLAKNIKLREICKELKGFSGADVELLCSRASLISISE
                     YIKNRNGELKIKQSHFDSALEYVKKKVKAVS"
BASE COUNT          668 a          450 c          403 g          637 t
ORIGIN      
        1 ctggttcagc tctcttacat ttcccatatc catattctcg ggaaacctgc cctctgcgat
       61 tttttctgta gcaaatatga gttctttcac gggttttatt atcctgttag aaagaacata
      121 gaaacccaaa gacgcaataa tgatcaaaaa aagcactatc catatggaaa cctgctgctg
      181 gccgcttaaa atattgctcc ttagagaaga aagcggctga tatataagga tcaccccttt
      241 tgagtttgcg ctttttatgg gtgaggcgat tttgagtgct tcatgaaact ttatgggcgg
      301 aagattaaaa cccttaagct caacaactgt ttcaccggtt ctcattacat ccagtatttg
      361 agggtctgta actacgattt gttttcgatt gttagtagtg gaaaatgccg ttcttctcgt
      421 tccatccgcg atcactcccc atgcgcctgt ttcgagggta ttctctaaaa atattattcc
      481 tgatggaaca tcacctgagc tgtagtatac attttcaaag gtctctatta tagatttaag
      541 tccctcggtt tttccactga gagcaaacct ctccatcaca ttaaaagcta tgagggaaat
      601 aagcagcatt gcaacggcgg ttataaaaag tatggtaaat aaaatctcta aacttaagcc
      661 cacctgcctt ttatcattca tagaagtaga taattaataa agcgattgtg atttaatcta
      721 tgaagtaatc taaactcaaa tcaccgcaaa aacaatacag cgttgccgca aatgagataa
      781 atgggccaaa gggaatagca tatttcagat cacctttatt gcgaagcatt attcctattc
      841 cgattatagt tccaatgagt gagcttatta gggcaacaaa aattatgctt ctccagccaa
      901 gaaacgctcc caccattgcc ataagtttaa catctccaag ccccagtccg tccatctttc
      961 ttacattttt gtatgcaaac ccgattagca gtaatgagcc ggcgccaata aatgcgccaa
     1021 atacagagct gaatatcctt acttcatcaa gcacatacag gatataaaga aaattgtaat
     1081 gaagattgtc caagagaaac cttagcgagt cataatcggt aataagagca ctgtatatga
     1141 gaccgacaac tacaccggga tagcttatta catttggaat aatcatgtgt tcaaggtcta
     1201 taaaggtaat tacaataagt gacatcacaa atataaacag aaacacgaag tttatagtag
     1261 gtccgtattt aataaatagc agaagtgata acaatccggc aagaaattct acaagcgggt
     1321 agtcccaact gtatttttcc ttacagttat aacacctgcc acccagcaca aggtagctaa
     1381 taatgggaat gttgtgataa aacggaatgg gttttttgca tgaagtgcag tgcgacgacg
     1441 gaaaaaccag tgaaatatca tttggcagcc ggtatatgca aacattaagg aaacttccca
     1501 cggctgcacc aaatataaaa gaaaatatca gaagtattgt gtttggatcc actcaaagtt
     1561 cccggaatgt gacatgaata tcaggatacg gcctttacct ttttctttac atactcaagt
     1621 gccgaatcaa aatgagactg ctttattttg agctcgccgt ttctgttttt aatatattcg
     1681 cttattgata tgagagaggc tctgctgcat agaagctcta catctgcgcc tgaaaatcct
     1741 ttaagctctt tacagatctc ccgcagcttg atgtttttgg ccaggggctt gcctttagta
     1801 tgaactttta gtatttcata aagtgctttg tcgttcggta caggaatttg aataaatgca
     1861 tcaaacctgc ctgacctcaa aagtgcggga tcaatcatat cgggtctgtt tgtggctgca
     1921 acgataaata ctcctctaag ctcttcaata ccgtccattt ctgtaagcag ctgacttact
     1981 attctttcag aaaccctgtt taagctgttt tccagcctgc atggggcaag cgcatctatc
     2041 tcgtcaaaga aaagaatgca gggtgaaacc tgttttgctt ttttaaaaag ctctcttaca
     2101 gcctgctctg actcgcctac aaatttagac aagacttcag agcctttaac tgaaatga
//
LOCUS       DBNGSV010000377         2298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1730168, whole genome shotgun sequence.
ACCESSION   DBNGSV010000377 DBNGSV010000000
VERSION     DBNGSV010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1730168"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1236
                     /locus_tag="AC159U_13435"
     CDS             256..1236
                     /locus_tag="AC159U_13435"
                     /inference="COORDINATES: protein motif:HMM:NF027559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HIR3338098.1"
                     /translation="MKNYQISILTAVFIFISFSLSNNSYSTDIAGSKDHPAVSRYQGS
                     VIKKYDYRQFDEYNLLLGKVVHAVGEPDNKKITNGKKLEGEITRITYELAEDRSSLEV
                     MKNYEIELEKSGAEILFSCSNKGCGGRTFNHTVVPYDTTFGDNYSDQRYLAAKLKGSR
                     SEFYISLYVINNKSGGGRTKNFIYTQLDIIEVVAMQKGMVTIDADAMQKAILESGSVS
                     IYGIYCDFDKTTIKPESEQSLSEIAILLTNNPSLNVFIVGHTDNEGSFDYNINLSQKR
                     AEAVVSKLITSYGIYGSRITPKGVGMLAPKASNSSEDRRSKNRRVEFVEK"
     gene            complement(1250..2161)
                     /locus_tag="AC159U_13440"
     CDS             complement(1250..2161)
                     /locus_tag="AC159U_13440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3338099.1"
                     /translation="MNTRNSLNITVVITTKNRGDTICCALESIFVIDYKDFNVIVVDQ
                     STDNSTAKAVSSYLDNPLFTYIKSDSVGSSAGRNIAIENSQTEFVAITDDDCEVSENW
                     LENMIEAFKADEKIGIVYGKVEPGRTDDPGFITTYIEDSSYISRSIYDAKSLRGLSAN
                     MGIRKSVWQMLNGFDPMLGAGVAFNSCEETDLNIRTLLNGYYIHYSPSVSVKHLGYRE
                     SSQVQKLIEDYAYGNGALFAKHIKSGHISIIFVLLRQISLWAFGKSWVMTYTNSGTYK
                     LGRMRAFLRGLIRGFSTPVDKSKEHYK"
BASE COUNT          789 a          399 c          390 g          720 t
ORIGIN      
        1 aatctgatat attatgtaaa ggaacaggag tttaggttta ataacagaca tcttagtact
       61 gacgaaatga tgaataaaat aattaatatt ttaatgaatt ctactccgcc agatgtctaa
      121 tgccctagat taatagtata aaaaaattaa tttcttatta ttagtattat tagatatttt
      181 taatgaacta aagatgcatc aaatttaacc cagcatatta acagttttgt tttaatttaa
      241 gtgggaggac aagtgatgaa aaactatcaa ataagcatac tgacagctgt atttatattc
      301 atatcatttt ctttaagcaa caactcatat tccacagata ttgcaggaag caaagaccac
      361 cctgcagtgt cacgatatca gggatccgta atcaagaaat atgattacag gcagtttgat
      421 gaatataatc tactccttgg aaaagtagtt catgcagtcg gtgagccgga caataagaag
      481 ataactaacg gtaagaaact tgaaggggaa ataacacgta taacttatga gcttgcagaa
      541 gacagatctt cactggaggt catgaaaaac tatgaaattg agctagaaaa gagcggagca
      601 gagatacttt tttcatgcag caacaaaggg tgcggaggaa gaacattcaa ccatacggtt
      661 gtaccatatg atacaacatt cggagataac tactctgacc agagatatct tgctgctaag
      721 ctaaaaggat cgaggagtga attttatatt tctctttatg tgattaataa caaatccggc
      781 ggtggacgta ctaagaattt tatttataca caattggata taattgaagt tgtagctatg
      841 caaaaaggta tggtcacaat tgatgcagat gcgatgcaaa aagccatact ggaatcggga
      901 agtgtatcta tttatggaat ctattgtgat tttgataaaa ctactattaa acctgaatcc
      961 gaacaatcac tttcagaaat cgcaatccta ctcacaaata atccttctct taatgtattt
     1021 attgtaggcc acacagataa tgagggaagc tttgactaca atataaatct ttctcaaaaa
     1081 cgagcagagg ctgtggtttc caagcttata acaagttatg gaatatatgg ttcaagaata
     1141 accccaaagg gagtaggtat gcttgctcca aaagccagta attcatctga agaccggagg
     1201 tctaaaaaca ggagagtgga gtttgtggag aagtaaaaat ttatgtgggt tatttgtaat
     1261 gttccttgct tttatctaca ggggtactga agcctctaat aagacctctt aaaaacgcac
     1321 gcatccgccc taatttatag gttccagaat ttgtgtatgt cataacccag ctttttccaa
     1381 atgcccataa acttatctgt ctaagtagta caaagataat ggaaatatga ccagacttga
     1441 tatgctttgc aaacagcgct ccgtttccat aagcatagtc ttcaataagt ttttgtacct
     1501 gactactctc tctgtatcca agatgtttta cagacactga agggctgtaa tgtatataat
     1561 atccatttaa taatgttctt atatttaaat cagtctcttc acaggaatta aaagctacac
     1621 ccgcaccaag catcggatca aaaccgttta gcatttgcca aacactcttt cttataccca
     1681 tatttgcaga aagtcctcta aggcttttag catcgtaaat gcttctagat atatatgaac
     1741 tatcttctat gtaagtagtt ataaatccag gatcatctgt tctgccaggc tctactttgc
     1801 cataaactat tcctattttc tcatctgctt taaatgcctc aatcatattc tcaagccagt
     1861 tttctgaaac ttcacagtcg tcatctgtaa ttgcaacaaa ctcagtctgt gagttttcta
     1921 tagctatatt tctacctgca gaacttccaa cagaatctga tttgatatag gtaaaaagag
     1981 gattatctaa atatgatgaa acagcttttg cagtgctatt gtcggtactt tgatcaacaa
     2041 ctattacatt aaaatcttta taatcaatta caaagataga ttccaatgcg caacaaattg
     2101 tatcccctct gtttttagtg gttattacta ctgtgatatt aagagagttt ctcgtattca
     2161 aatttacaaa aattcacgtt tattattacg aattgagatt attttattat atagccatga
     2221 tattaatgtt ccaaaaatag atcccgcagc aaagccatag acaaatccga taatacttcc
     2281 gaaaaaggtt actttata
//
LOCUS       DBNGSV010000378         9313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1038133, whole genome shotgun sequence.
ACCESSION   DBNGSV010000378 DBNGSV010000000
VERSION     DBNGSV010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1038133"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC159U_13445"
     CDS             <1..440
                     /locus_tag="AC159U_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338100.1"
                     /translation="TADYSFEVTYDDKGYFAFVLPPGKYYFLQFDYWGIIPGRGLQSA
                     EAFRTYMSLPGFREIKRPYLITIEILPNSVSYIGTIKHRVNRYSRRDYRFWIEIVDEY
                     EDTNKWFTNSRFSTDKDLVKNIATVTYIISDKLPSPPNSITSE"
     gene            complement(724..797)
                     /locus_tag="AC159U_13450"
     tRNA            complement(724..797)
                     /locus_tag="AC159U_13450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Arg,seq:cct)
     gene            complement(931..1233)
                     /locus_tag="AC159U_13455"
     CDS             complement(931..1233)
                     /locus_tag="AC159U_13455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3338101.1"
                     /translation="MKNTEIIYDQRTSERFPLITKIVILLNSGENIIASTFNVSDNGV
                     GMNSNLDIPVGTKIRARIYLEKETLGVNGQIKWSSKNPDSSFDYGIQLNESIFYKD"
     gene            complement(1337..1468)
                     /locus_tag="AC159U_13460"
     CDS             complement(1337..1468)
                     /locus_tag="AC159U_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3338102.1"
                     /translation="MSHGVDLVTVKEFLGHSSISTTMIYSHLSTQHKINSIEKLPWS"
     gene            complement(1770..2039)
                     /locus_tag="AC159U_13465"
     CDS             complement(1770..2039)
                     /locus_tag="AC159U_13465"
                     /inference="COORDINATES: protein motif:HMM:NF028182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HIR3338103.1"
                     /translation="MFDYVDESNIDCKSRYARYLSGLLSSHELLMIFYSSLHGAERGK
                     FKPLIEEYAIFENLDLSELVNPYNHVPLFDKSAYGSQEVGQYFNK"
     gene            complement(2237..2503)
                     /locus_tag="AC159U_13470"
     CDS             complement(2237..2503)
                     /locus_tag="AC159U_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338104.1"
                     /translation="MGAVWRLCGRVNPILSFLALVAVLIAIVLQNKELQYSREELRRS
                     SQALEEQSESFRVQNFENTFFQMVRLHHDIVGGIDLSELDLNKS"
     gene            complement(2470..2709)
                     /locus_tag="AC159U_13475"
     CDS             complement(2470..2709)
                     /locus_tag="AC159U_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338105.1"
                     /translation="MNDDKKNNTDDVKDVIKKEPSKTRYSDTIVWVLIGVAALAVISF
                     ISALYYYDCHVTQGIEGLSGVKREEWGQFGDYVGG"
     gene            complement(2970..3635)
                     /locus_tag="AC159U_13480"
     CDS             complement(2970..3635)
                     /locus_tag="AC159U_13480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3338106.1"
                     /translation="MCGRFTLFDNAEKIEDEFEVEIQPDLCAKSYNIAPSQDCLVILT
                     DEDRRVCTQMRWGLVPFWAKDSKIGYKMINARAETVDEKPSYKKPLKDRRCLVITNGF
                     YEWKKEGAKTKTPYFIRLKNKRPFTFAGLWDRWDKEGEQMRTFTIITTDANEMMAQIH
                     DRMPVILSGEGRYKWLDPDIKDAAELKQLLVPYPSEEMEAWPISTIVNSPRNNGTECI
                     EPV"
     gene            complement(3702..4828)
                     /locus_tag="AC159U_13485"
                     /pseudo
     CDS             complement(3702..4828)
                     /locus_tag="AC159U_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612266.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5185..6003)
                     /locus_tag="AC159U_13490"
     CDS             complement(5185..6003)
                     /locus_tag="AC159U_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338107.1"
                     /translation="MEQFKFACTSLAVFALLSVSCLAQQPESEAEQAESKTQQPVLQD
                     DKTLSEKKYDLFKTANFIHINSQQVEKWHEELPDSDNPNLADKIIETEVAIERRKEHF
                     KELTNIDYTTHEDFFSWWEENADYLEWIISLNKLAVNEEAKSEGTVLNHFKYVFELEG
                     WQYWSFMAQNRVKESRQSGEFIKAYVHNKGSVRVKKSQLEDPLQKERGYKDAADILIN
                     KKLITAEEHLPLIELLTKFTGEKFEDYSAWVFWWDTNKNRLKLNEEGTRLIVNQ"
     gene            6438..6662
                     /locus_tag="AC159U_13495"
     CDS             6438..6662
                     /locus_tag="AC159U_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338108.1"
                     /translation="MEAQGCDGDSIFIGVGETCEPNPCLGEVPAPDPDPTVIVSTMGQ
                     WGIIFASIVLGAIGIVAIIREKNMGRYMDE"
     gene            6761..6949
                     /locus_tag="AC159U_13500"
     CDS             6761..6949
                     /locus_tag="AC159U_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338109.1"
                     /translation="MAKALNWYSWYRQEMEVPQRLLQYLMILAEDDCILNYLDSNKAR
                     SRDEFMACTLYLSRWVFN"
     gene            7798..8127
                     /locus_tag="AC159U_13505"
     CDS             7798..8127
                     /locus_tag="AC159U_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338110.1"
                     /translation="MDNEINNKNNSKQVKIVGVAAGVLILILLAVSVFQRMKINELNS
                     LYDEKIEKIRDKGQKEVSRVYYKMVALRYDYKKLEESVNKYKENLRSLDPETVKSLEL
                     LLRLLRV"
     gene            8172..8753
                     /locus_tag="AC159U_13510"
     CDS             8172..8753
                     /locus_tag="AC159U_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338111.1"
                     /translation="MDPQVAIKEMQKQMEELEQTNKKLDELLAWNAVGASISDPDKMF
                     GVIRIEDLQQRAHRLELKSIALADGEEKKTFDLKNGEKKKVEEGWYVYEFMLESKADT
                     RIGALEINCKTENEAEILDVEFKTEANARNMEQGYVFENAKWNRELPKRTGWTARIGL
                     MSPHDIKLNVITSTDPGDIECRYSVEQRQGAGS"
     gene            8775..9095
                     /locus_tag="AC159U_13515"
     CDS             8775..9095
                     /locus_tag="AC159U_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338112.1"
                     /translation="MEYIYVLYSEILICFIVIAVSVYLLKRSGKSKGYLITFTTVLVI
                     GLGILLYLEVAEKRANEDLQKATEQLQNESEKYTEEVQKLQEENRKLKEQIKEEKQSE
                     NLDR"
BASE COUNT         2792 a         1895 c         1751 g         2875 t
ORIGIN      
        1 caactgctga ttattcattt gaggtaactt acgatgataa gggctatttt gcatttgttt
       61 taccaccagg aaagtactat tttcttcaat ttgattattg ggggattata ccaggcaggg
      121 gcttgcaaag tgcggaggct tttaggacat atatgagtct tcctggcttc agagagatta
      181 agcgtcccta cctaataacc attgaaattc ttcctaacag tgtttcatat atcggcacta
      241 taaaacatag agttaataga tattctagaa gggactatcg cttttggatc gaaatagttg
      301 atgaatatga agacactaat aaatggttca caaattcaag attcagcact gacaaagacc
      361 tagttaaaaa catagcaacg gtcacttata taatttctga taaactgcca agccccccaa
      421 atagtatcac ttcagagtaa taaattcatc atattttagt ttaatgaata ggagtaactg
      481 tatgagtgtc gctaaacgga atattcctta gcaaggaacc aaaattgtag acacctattt
      541 ggacaccaga cctgaagaag atttaaaaaa agtgatggaa gtaattgatt tacaaaccaa
      601 tgaaacattt ccattggaga taataatttg attcatggac aattttgcaa ttaaactgta
      661 gcaaaaactg tagcaataaa tgaatgtaag attttagagc gttgtaaccc cctgaaatta
      721 ttgcgtcccc gactggattc gaaccagtgg cctcaggatt aggaatcctg cgctctatcc
      781 tactgagcta cggggactat ccaataactt cagtgagtta caagatacca tattccaatt
      841 cccttgcaat ttctagctat attctaccca actagagtca gcttaagcta aatccctcat
      901 gatccagcta ttaaaaggct ggataattta tcaatcttta tagaatatag attcgtttaa
      961 ttggatacca taatcaaatg aactgtctgg atttttagat gaccatttaa tttgaccatt
     1021 tacccctagg gtttcttttt caagatatat acgtgctctt attttagttc caacaggtat
     1081 atccaaatta gagttcattc ctacaccatt atcagatacg ttgaaagtgc ttgctatgat
     1141 attctcccca gaattaagta atataacaat ctttgtaatt aaaggaaatc tttctgaagt
     1201 tctttgatca taaattattt cagtattctt catactatcc tcccgtaact atcgacaatc
     1261 attatacttt atttaatcaa tacagtaaac aggataatct attttatgat atagtgctct
     1321 attcaatgca tttatttcaa ctccaaggca atttttctat actgtttatt ttatgttgtg
     1381 tagataaatg tgagtagatc atagttgttg atatagagct atgaccgaga aattctttca
     1441 ctgtaacaag atcaactcca tgagaaatgg ctgggtcatt ttgatattaa gaacactatg
     1501 gtgtatctat agattctgag tcaggacacg agagactttt atgaggctct ggagttttga
     1561 taatatattt aacaattcat taatcccaat ataaacaaga aaataataaa ttgtagaatc
     1621 ccgtgattaa gacgatgaga attcaaatct tcgtattcgt ttagattact aaaaatatgc
     1681 tttataacga tatataataa taggtaagat ctagaagttg aaaatcaaca tatagaaata
     1741 aactttaaga tatcagaaag atatgaattc tatttattaa aatactgtcc gacttcctgg
     1801 ctaccgtagg ccgatttgtc gaacaggggc acatgattat atgggttaac aagctcgctt
     1861 aagtctaggt tttcaaatat tgcatactct tcaattagcg gtttaaactt accccgctcc
     1921 gcaccatgaa gtgagctgta aaaaatcata agaagctcat gcgatgacag taggccgctt
     1981 aggtagcggg catacctgct cttgcagtca atattactct cgtctacata atcgaatatg
     2041 ttatatagag gtttctgaaa tactggccca cgtcgtgctc ctgctcgaac ataaacgcca
     2101 aaaacgcatc ctctattatc tgcagcgtct gctgctcggt gaggttagct cccgtgcccg
     2161 aatcatcttc aattttcctg aatacctgat atttttcgca aaaagctgtt tcgtaaaaat
     2221 atttaaaaca gtcccttcac gatttattta aatctaattc actaagatca atcccaccca
     2281 ctatgtcatg atgcaggcgc accatctgaa agaatgtgtt ctcgaagttc tgcaccctga
     2341 aagactcgct ctgctcctca agcgcctgtg atgagcgcct gagttcttca cgagaatact
     2401 gaagctcctt gttctgaagt actatggcta tgagcacggc tacaagcgcc agaaaactga
     2461 gtatggggtt tacccgccca cataatcgcc aaactgcccc cattcctcgc gctttactcc
     2521 gctaagcccc tcaatgccct gcgttacatg acaatcataa taatacagcg ccgagataaa
     2581 cgagattacc gccaacgcag ccactcctat gagcacccat actatcgtat cgctgtatct
     2641 ggttttactt ggctcctttt taatcacgtc tttaacatca tcagtattat tttttttatc
     2701 atcattcata gcgttttatt ttaatactcc cctcactttc aatcaagagg gttttgtaac
     2761 cttatgtgat cagaggttct ttttcaggtt attaactgcg agattgacac gtcgaaactt
     2821 ttaaaaaaac tttaaggtct tacacacttc accttaacat taaagtcagt tctcgcaatg
     2881 acagttgtgg atagtaatag ccatacaata ctattaataa ctaaaaaaaa cagcagattt
     2941 ctcatataca ttcgagctga catgcccatc taaacaggct caatacactc cgtcccattg
     3001 ttccttggag aattgacgat ggtggaaata ggccaggctt ccatctcttc tgatggataa
     3061 ggcacaagaa gctgttttag ttcagcagca tcctttatat caggatcaag ccatttatac
     3121 ctgccctcac cgcttaatat cacaggcatc ctgtcatgaa tctgcgccat catctcgttt
     3181 gcatcagtgg tgatgatagt aaatgtccgc atctgctccc cctctttatc ccacctgtcc
     3241 caaagccctg caaatgtaaa tggtcttttg tttttaaggc ggatgaaata cggagttttc
     3301 gttttagccc cctctttttt ccactcataa aacccgttgg taatcactag acaccttctg
     3361 tccttaagcg gctttttgta gcttggcttc tcatccaccg tctctgccct tgcgtttatc
     3421 atcttatagc cgattttact gtcctttgcc caaaacggca ctaaccccca gcgcatctgt
     3481 gtgcacaccc ttctgtcctc atctgtaaga atcacaagac agtcctgact gggtgcaatg
     3541 ttgtagctct ttgcacaaag atcgggctga atctctacct caaattcatc ttcaatcttt
     3601 tctgcgttat caaataatgt aaaccgtcca cacataaaat taattgacct taccccttat
     3661 aatagtacca gataataagt tagaatatca ggtactgccc ctcacccaaa agctgtacca
     3721 ggataactag agatttccgg cttttttatg tgttgtttaa catactcctc cggcgtcata
     3781 ttatcaagtg ccgaatgagg tctgtactct ttgtaatctc tcctccaggt ttctatcttt
     3841 tcccttgcat catcaagtga aagaaaccaa tgagtgttca aacactcatc cctaaaactt
     3901 ccattgaatg actctatata ggcattatca gtgggttttc ccggtcttga gaagtcaagc
     3961 accaccccat tctcatatgc ccacttatcc aatgctttgg atataaactc actgccgtta
     4021 tctacttgta tcctctcagg aacccttccc ataaaacgtt ttaaagcttc cataacttct
     4081 accaccttgt ctgcttttat cgagtgatcg gcatgtattg caagacattc acgactaaaa
     4141 ttatctacta tagttaatgc ccttatccgt ctgccattga acagactatc acacacgaaa
     4201 tccatagacc atgtctgatc tattttatat acaacaggcc tcatttcccg gcaggcagca
     4261 gatgcccttc tcttaggacg tttcctcctt aaattaagcc cgtcttcaca gtatattctg
     4321 tatactttct tatgattaac atgccagccc tctcgcctga gcaatatgtg gatgcgtctg
     4381 taaccgtacc gtactcttgc ctctgcaatc tcaacaatac gctttctaat tgctcgatca
     4441 tcccttacag aacggtagta ataagtactc cttcccatct ttattactct gcatgctctt
     4501 ctcatcccca cacggtaaac cctatccaca tactcagcta agtttctctt aacggctggc
     4561 tttagagctt ttttgctatt acatcctgaa gcatatgctt atccaggctt aaatcagcta
     4621 ctagtttctt aagctgtctg ctctcctcct caagctgctt cagtcacctt agctcactca
     4681 ctcccaggcc actgtacttt ttcttccagt tgtagtatgt ggcctctgct actcccattt
     4741 tcctacatat ctccgatact cttgtccctg tctcggcctg ctttaaagca aaggctatct
     4801 gttgttctgt atatcttgac ttcctcatgg caaaatacct cctgatttat tgcattattt
     4861 tgccagattt ctctacttat ggctggtgca gttttcaggg agaaggtcat aaaatgcaac
     4921 tataaaaaaa gagccagacc acttcgggaa gcagcctggc tcagtatcat gtaaatcctg
     4981 ggaggattta ttacatgaag tttatattat atcacatttt taaattaaaa tcctctgttt
     5041 ttctcaattc tcaacccact gctcaaagtc ttcttaaggt cctttttgct ctctataaaa
     5101 gttttgtatt cattatgggt ctaggcttat tataagcggg cttgataaat acaagcccga
     5161 atatttagag tctgcactga acaattattg attaactata agcctcgttc cctcttcgtt
     5221 cagtttcagc ctgtttttgt tcgtatccca ccagaacacc caggcgctgt agtcttcgaa
     5281 cttttcgcct gtgaatttgg ttaaaagctc tattaaaggc agatgttctt cggcggttat
     5341 aagcttttta ttaattagaa tgtcggcagc gtccttgtat cccctttctt tttgcagagg
     5401 gtcttcaagt tgtgatttct ttaccctcac agagcccttg ttatgcacat atgcttttat
     5461 aaactcgccg gattgccggg actcctttac cctgttttga gccataaaag accagtactg
     5521 ccagccctct aattcaaaca catatttaaa atgatttaaa actgtgccct cgctctttgc
     5581 ctcctcattt acggcaagct tgttgaggct tatgatccat tccaagtaat cagcattttc
     5641 ttcccaccag ctaaagaaat cttcgtgggt tgtgtagtca atatttgtaa gctccttaaa
     5701 gtgctctttg cgcctttcta tagccacttc agtttcgatt attttatctg ctaggttcgg
     5761 attgtccgag tccggcagtt cctcatgcca cttttccact tgttgagaat ttatatgtat
     5821 aaagtttgcc gttttaaaga gatcatattt cttttcactg agtgtcttat catcctgaag
     5881 caccggctgc tgtgttttac tctctgcctg ttctgcttca gattcgggct gttgggcaag
     5941 gcatgaaaca ctcagcagtg caaacaccgc aaggcttgtg caggcaaatt taaattgttc
     6001 cattttagag ttccttataa catctgctat aaaatgtatt atatcttccg gtatataatt
     6061 tttcgtatca acatattacc aaatggatat ttaatgtcat gttagatttg tattagtgaa
     6121 gaatctgcac ttctacaatc ttgatgtgaa acgggagagg ctttcattgt tccgttttct
     6181 tattaaaaga atcgttgtca aataaaacac aatttagacc ccatttatac ttctatagta
     6241 tctcaactgt aattataaat ggattgcaca taatactgaa aataatataa tcggataatt
     6301 attatttagg agatctgaga tgagaaaatt aatgttgtaa atatttgtgt tataggcttt
     6361 gaggtttact gccgtgaatg attccggagc aggcacgagg actcccctgt atagatggtg
     6421 gagaggtatg cgagttcatg gaagcgcaag gctgcgatgg agattccata tttatcggtg
     6481 ttggagagac atgtgagcca aatccgtgtc ttggtgaagt gccagctccc gacccagatc
     6541 caacggtaat tgtttcaaca atgggacagt gggggatcat ctttgccagt attgttctcg
     6601 gagcgattgg aatagtagcc ataattcgtg agaagaatat gggcaggtat atggacgagt
     6661 aacactttaa tataataata attaaaacta tgccccgctt tcggaattat ctgtcagcgg
     6721 ggtttttttg tgtcaaaact attatgtcat atcgggagta atggcaaaag cgcttaactg
     6781 gtactcgtgg tacaggcagg aaatggaggt gccgcaaagg ctgctgcagt atttgatgat
     6841 ccttgcagag gacgactgta ttcttaatta cctcgattct aacaaggccc gctcgcgcga
     6901 tgagtttatg gcgtgcacat tatatctatc taggtgggtt ttcaattgaa gacataaagg
     6961 ggtctgtttg ttttaagaaa aagggagtga cattaataca gatagataaa tggatagaag
     7021 ggaacaaaat atatcccggt atggagtaca ttctcctgca aaatttttgg gagaagaatc
     7081 ggttttactg tgagttttgc gacgggaagc ggtatgacct ttccggtcgc attacccctt
     7141 accccccctc atggcagtcc ttatacttgc agggagaggg gtagggttca gctttgaacc
     7201 caatgttctt ttcctggatg aaaagaacca aaaatcaccg gcttaataat aattgctaaa
     7261 aattaattat ttatcctaaa ccgtacttgc caaattagtt tttcagagga ttggtattca
     7321 catatattaa ttactttcaa tatgggccaa acatttcctt aacaaaaatg tttgttcacc
     7381 cctctgcccc acaccgcttc ttaacagcta aacaataaat cttttaaacg caattcttat
     7441 aatgccggaa ctgcaaaagc aaaagataaa ataaaaacaa acttcctaca actgttatct
     7501 cgaatgtata tgagagatct tttctaagtt attatcccat tgtaggaaga ataagatttc
     7561 tcgctctgcc cgaaatgaca caccatttga aagaatacct tatacataat gatattatta
     7621 cactttttta ttatggatga tgctatcaga ttaaataata ttatttttct tataaatact
     7681 cttgtgatag tgctggctct gggacccccg tccggacaaa aaaaataaat agcagtgtct
     7741 tggctgtgct aaatttaaag ttataaaaaa atctgaatac tgttgtataa taggtccatg
     7801 gataatgaaa taaataataa aaataacagt aaacaggtaa agattgttgg cgtggcagcg
     7861 ggggttttaa tactcatact attggcggta agcgtgtttc agcgcatgaa aattaatgaa
     7921 ctaaacagtc tgtatgatga gaaaatagaa aaaataagag acaagggcca aaaggaagtg
     7981 tcgagggtat attacaagat ggtggccctg cgctatgatt acaaaaagct tgaagaaagt
     8041 gtgaataagt ataaggaaaa cctgcgtagt ctagatcctg agactgtaaa gagcctagag
     8101 ctgctgctgc ggttactgag agtataaagc cagcagaact agggcagatt gatccgaaac
     8161 agtttgagaa tatggacccg caggtagcga taaaagagat gcaaaagcag atggaagagc
     8221 ttgagcaaac gaacaaaaag cttgatgaac tgcttgcatg gaatgcagtc ggggccagta
     8281 tttccgatcc cgataaaatg tttggagtga tacgaataga agacctgcag caaagagcgc
     8341 acaggcttga actaaaatct atcgccttag cagatggtga agaaaaaaag acatttgatc
     8401 ttaaaaatgg tgagaagaaa aaagtcgaag agggctggta tgtatatgag tttatgcttg
     8461 agtctaaggc cgatacgaga ataggcgcgc ttgagataaa ctgtaaaacc gaaaatgagg
     8521 cagagatttt ggatgtggaa tttaaaactg aggcaaatgc caggaacatg gagcaggggt
     8581 atgtgtttga aaatgcaaag tggaaccgcg agcttccaaa aagaacaggc tggactgcaa
     8641 gaataggcct tatgagcccg catgatatta agttaaatgt aatcactagt actgacccag
     8701 gagatataga gtgcagatat tctgtggagc aaaggcaagg ggcggggtca taggccggtt
     8761 agtattaagc agtcatggaa tatatatacg ttttatattc tgagatttta atatgcttta
     8821 ttgtgatcgc cgtaagcgtt tatctgctaa aaagatcggg caagagcaaa gggtacctta
     8881 taacctttac cacagtcctt gttataggac ttggcattct gctgtatttg gaagttgctg
     8941 aaaaaagggc aaacgaagat ctgcaaaagg ccaccgagca attgcaaaat gagagtgaga
     9001 aatacaccga ggaagttcag aagctgcagg aggagaacag gaagttaaaa gagcagataa
     9061 aagaggaaaa gcagtctgag aacttggata gataaacaca gtttatttta tatgtcatct
     9121 cgaacgcatg tgagaaatcc ttttatgtga ctgtcccaaa gcagaagaaa aagatttctc
     9181 acctccggtt cgaaatgggc ataattgcaa aaaatgtgtg ctgaaaatgg ggttttagcc
     9241 tttatttact atattataat gagaaaaaag ttaaacaggt ttaactatga acaaaaaact
     9301 gtcccctttg cag
//
LOCUS       DBNGSV010000379         9220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_414202, whole genome shotgun sequence.
ACCESSION   DBNGSV010000379 DBNGSV010000000
VERSION     DBNGSV010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_414202"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..176
                     /locus_tag="AC159U_13520"
     CDS             27..176
                     /locus_tag="AC159U_13520"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3338113.1"
                     /translation="MLGAMVGGYISTQLGFGSFSGFNLGSIVIAVGGAMLLLILYRVI
                     KGKKI"
     gene            complement(193..546)
                     /locus_tag="AC159U_13525"
     CDS             complement(193..546)
                     /locus_tag="AC159U_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338114.1"
                     /translation="MPTDQKIDYIEFPASDLNAVQEFYEKTFGWKFTDYGPEYRAFTD
                     GKLDGGFYKSDKHSVTENGAALIVLYASDLEETQEKIISNGGKIVKETFSFPGGRRFH
                     FSDPNGNEIAVWSDK"
     gene            complement(571..1653)
                     /locus_tag="AC159U_13530"
     CDS             complement(571..1653)
                     /locus_tag="AC159U_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HIR3338115.1"
                     /translation="MRSGLAQLPLHGGKAPHWLFSRMTRLAREITCHIVDEFGPREML
                     GRLSDPYWFQAFGCVLGFDWHSSGVTTTTCGAVKEGIKELESDLGFYACGGKGATSRK
                     TPSEINTKCDKLAKEPDDLVYASKMSAKVDNSALQDGFTLYHHCFFFTDDGWTVVQQG
                     FDESKRTARRYHWISSELDSFTVEPHSAICCDKTKPTLNLVANESKDNTSIMTELANL
                     PPDKLQKDLQKLPNLVMPDRHPVTIEDVNSRYLKKIFEKTYESAPKDFESLLGIEGVG
                     PKTLRALSLTAEIIYEAKVSTKDPARFSFAHGGKDGTPYPVDINTYEKTIEVLSNALD
                     KSRVDRSEKIKALKRLGRFSGGYATA"
     gene            1726..3057
                     /locus_tag="AC159U_13535"
     CDS             1726..3057
                     /locus_tag="AC159U_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3338116.1"
                     /translation="MTNEINKKNIESLVPCIKVKDVKAAMEYYICVLGFSPRYYIEGK
                     PESGGVSINELSVDFISGEGKNSDTSLNFLVNDVDELFEFYKSRNAQIIFKPQDSGGM
                     RLFGIRDLDGYRLFFYRWTKSDPHRERRKTKVEKEIPERFKDSLMNSATSAVKSGDTE
                     VLKKLLSIEPSLVQWTTPASYLQNKQLTLMHVLIDYRSGRFNEHSADVAKLLIASGAQ
                     VDALDGVTSGNTPLQMLSSITGNHSYMLDIGTVLLEAGANPKDAISNDWSMSAFACAL
                     LNGQTRLAELMFPKVQRQGFGWIAGLGNLELVKSFFATRDSDEYKKYSIHWAQEETDP
                     EFDLSKNKSSLIAAYLVSCINGKTEVAEFLLEKGVDVNVLVTGTDFAGIGGTGLHWAA
                     YHGHFDTVKFLVSNGADIYAEDDAYALTPAGWAGRNDHYEIADYLLELRCS"
     gene            3132..4223
                     /gene="amrS"
                     /locus_tag="AC159U_13540"
     CDS             3132..4223
                     /gene="amrS"
                     /locus_tag="AC159U_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR3338117.1"
                     /translation="MKERSFIDTYTIKGDWWHTLEDGRIQCDLCPRNCRLKDGQQGFC
                     YIRESQNGEMVLTSYGRASGFCIDPIEKKPLNHFYPGSSVLSFGTAGCNLGCRFCQNW
                     DISKAREDDRLTSPASPEKIAKAAKEHGCKSIAFTYNDPVIFAEYAIDSSLAAHELDI
                     KTVAVTAGYITEEARGDFYKNIDAVNVDLKAFTQEFYGKICFAELQPVLDTLIWLKTE
                     TKVWIEVTTLLIPDHNDSNEEITKLSEWFLQNLSPDVPLHFTAFHPDFKMMDVPATPP
                     ETLSRARNIAKEIGINHVYTGNVYDTEGQSTYCHKCNNILIERNWYELGKWNLDKTGC
                     CNNCGEKLSGHFDKNPGDWGSKRLPVFIQ"
     gene            4220..5044
                     /gene="amrB"
                     /locus_tag="AC159U_13545"
     CDS             4220..5044
                     /gene="amrB"
                     /locus_tag="AC159U_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR3338118.1"
                     /translation="MKDIRPYLEQRWYPRDPDELSISIENYLNTAERDQIESKIYGII
                     VPHAGHIYSGQVAAYAFNCIKGVKPETVVIVSPSHYIGGADIIVSPHTSYSTPLGNID
                     IDTHLLDDILKRLYKEFDITILKYPEDPEHSIEVELPFLQVIYEDFKLIPIMIRNQDE
                     KVSKALGHVLADVLKDKISIIIASSDLSHYYNQKTANKMDREIINRIESLDPESVLDA
                     EEQGIGQACGIGAIATVLWASKEMGAKKAQILNYATSGDVSGDYDKVVGYVSAALY"
     gene            complement(5082..5804)
                     /locus_tag="AC159U_13550"
     CDS             complement(5082..5804)
                     /locus_tag="AC159U_13550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3338119.1"
                     /translation="MNDKKEERSEDDLQLNQLIVKSIVWMVLSFFILGGLLFGFAGTF
                     NWERGWIFIFTFFICIVVNLVILLKVNPEVIEERSRFHRDAKKWDMILMSIGSIFILG
                     TLVVAGLDEKNKWSELSGVVWLYLGVFLFVIGNLYILWAMAVNKWFSKLVRIQSERAH
                     RVVTEGPYSHVRHPGYVGWAVMWIAIPLILGSLWAFVPAVLSVVLIVIRTSLEDKTLQ
                     SELPGYMEYVSKVKYRLIPKLW"
     gene            6012..6491
                     /locus_tag="AC159U_13555"
     CDS             6012..6491
                     /locus_tag="AC159U_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3338120.1"
                     /translation="MAHILIYIINKTNSRGFIKMSISSFCNKEVICVEMGTSIEQVGN
                     LMEEKNIGCVVVIDSGKPCGLVTDRDILIRVINRGLSPAETYVDDVMSELVMTLDEDM
                     GLHEALEKVKGMSVRRFPVTDKSGNLKGSVTLDDIIYLLGKEMYDVASIIKSESAEL"
     gene            6607..7791
                     /locus_tag="AC159U_13560"
     CDS             6607..7791
                     /locus_tag="AC159U_13560"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3338121.1"
                     /translation="MQNGIVVRPLEQLLPHEQEIEIVERKGIGHPDTICDRAAEQVSI
                     ALCRLYLSEFGKIMHHNVDKALLVGGSTNPVYRGGKVLKPIEIIIAGRATMEKNGKVL
                     PVEEVAREAILNYLSRTIRNLNVENSIELKIKIRPGSSELTELFSRFGPEQVPFSNDT
                     SIGTGFYPFDETEEVVYSIEKFLNSHKIKKKYPFIGEDIKVMGVREHDNIKITLAVAT
                     VDKYINDLNDYIDKIKQIRELIKEQDWFKNSYEIYINTADSYEQESIYLTVTGTSGEH
                     GDDGQVGRGNRANGLITPYRPMTLEAVAGKNPINHVGKLYNLFANDLCKTIVDNGYAE
                     EAYVSLVSQIGKPLNEPQIVDIKVKEQKVEKPIIESTAIDMLGEMPQMWKKIIDGMYE
                     IA"
     gene            7839..8906
                     /locus_tag="AC159U_13565"
     CDS             7839..8906
                     /locus_tag="AC159U_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR3338122.1"
                     /translation="MEHEHTSDHACCHTPGDKKSIDYLFWGSLIIVVILCSIYFLFYS
                     VVENVPWLNTLSSSVLELLKTIWWGILLGALSVGLLSKIPREFIISILGNGGTLKGIL
                     RATLGGVFLDLCSHGILMVGAKLYERGASIGQVMSFLVASPWNSFSLTFILIALIGLK
                     WTLSFIVLSMIIAVISGYIFDTLVNKQTLPANPNRIEISKDFNFLSEAKKSFNNTKFD
                     SKLFLEIIKNGILESRMVVRWILFGVLLASAIRAFVPTEHFVHYFGPTLLGLGLTLVA
                     ATIIEVCSEGSTPIAADLLTRAGSPGNSFAFLMAGVATDYTELAVIKQTTQSWKITFL
                     LPILTIPQVFVVAYFLNSSVQ"
     gene            complement(8900..>9220)
                     /locus_tag="AC159U_13570"
     CDS             complement(8900..>9220)
                     /locus_tag="AC159U_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338123.1"
                     /translation="LELVILHDGRSEGGDKKSFLGTKDKSLIALTENSTKQTGINTDN
                     SVSHIAFEVGDIEKAKTNLESKRVKFIEIKKNSKGDPVAYHFLDPDGFELEIYNDTEK
                     VAPY"
BASE COUNT         2964 a         1770 c         1880 g         2606 t
ORIGIN      
        1 gttttatagc aactactata attggtattc tgggtgcaat ggtaggagga tatataagta
       61 cgcagcttgg ctttggcagc ttcagtggtt ttaaccttgg aagcattgta atcgctgttg
      121 gcggagcgat gttacttcta attctttaca gagttataaa gggtaagaaa atctagtatt
      181 acagagagca gttcacttat ctgaccaaac cgcaatctca tttccattgg ggtctgaaaa
      241 atgaaagcgc ctgccgccgg gaaatgagaa cgtttcctta actattttgc ctccgttaga
      301 tatgatcttt tcctgtgttt cttcaagatc agaagcatat aggactataa gtgcggcccc
      361 gttttcagtt acggagtgtt tatcagattt ataaaacccg ccgtctaatt tcccatcagt
      421 aaaagccctg tactcaggcc cgtaatcagt aaacttccaa ccaaacgtct tttcataaaa
      481 ctcctgcacc gcatttaagt cactagcagg aaattcaata taatctattt tctggtctgt
      541 gggcatgata tttttatata gatggtaaca ttaagctgta gcatatccac cgctaaacct
      601 acccaatctt tttaatgcct ttatcttttc actcctatca actcttgatt tatccagagc
      661 gttacttaaa acctcaatcg ttttttcgta tgtatttata tcaacgggat aaggcgtgcc
      721 gtcttttccg ccgtgggcaa aggagaatct ggcagggtct ttagtgctga ctttggcttc
      781 atagattatt tcggcagtaa gcgaaagggc tcttagggtc ttaggcccga ctccttcaat
      841 accaagcaga ctctcaaaat cctttggcgc tgattcataa gtcttttcaa aaatcttttt
      901 aagatatctg gagtttacat cctctatagt cactggatgt ctgtcaggca tgactaagtt
      961 aggaagtttc tgtaagtctt tttgcaattt atcaggcgga agatttgcaa gctcagtcat
     1021 gatgctagta ttatccttac tctcattagc aacgagattt aatgtaggct tggtcttatc
     1081 acaacaaatt gcactgtgcg gttctacggt aaaactgtcc agttcagaac ttatccaatg
     1141 ataccgcctt gcagtcctct tagactcatc aaaaccctgc tgtactacag tccacccgtc
     1201 atcagtaaag aaaaaacagt ggtgatacag agtaaacccg tcctgaagcg cagagttgtc
     1261 taccttagca gacatcttag atgcatagac aagatcatcc ggttccttgg ctagtttatc
     1321 gcactttgta tttatctctg agggagtctt tctggaagtc gcacccttac cgccacatgc
     1381 atagaaaccc agatcgcttt cgagttcctt tataccttct ttaaccgccc cgcatgttgt
     1441 ggttgtcact ccgctagaat gccagtcaaa cccgagtaca cacccaaacg cctgaaacca
     1501 gtatggatcg gacagtctac caagcatctc tctcgggccg aattcatcga caatatgaca
     1561 tgtaatctcc cttgccagtc ttgtcatccg gctgaaaagc cagtgcggcg ctttgccccc
     1621 gtgtaagggc agttgtgcaa gccctgatct cataagcaaa ttaaattgat ctcaaattta
     1681 ataacagtta tgattttaca ttattgattt tgagttgccc aaaaaatgac caacgaaata
     1741 aataaaaaaa atatcgaaag tctggttcca tgcataaagg taaaagacgt aaaagcagca
     1801 atggaatact atatctgcgt gcttggtttc agcccacgct actatatcga aggaaaacct
     1861 gaaagcggcg gagtgagcat aaatgaacta tcagtggatt tcatttcagg cgaggggaag
     1921 aactcagaca catctctcaa ttttctggta aacgatgtag atgaactgtt tgagttttat
     1981 aaaagcagaa acgcccagat aatttttaag ccgcaggact caggcggaat gcggctgttt
     2041 ggtattcgtg accttgacgg atacagacta tttttttacc ggtggacaaa gagcgacccg
     2101 catagggaga gaagaaaaac aaaagtagaa aaggaaattc cggaaaggtt taaggattcc
     2161 cttatgaatt cggcaaccag tgcagttaag agtggggata cagaagtgct aaaaaaactt
     2221 ctaagtatag aaccttctct tgtacagtgg acaactccag cttcatattt acaaaataag
     2281 cagcttacac ttatgcatgt gctaatagat tacaggagtg gaagattcaa cgaacattct
     2341 gccgatgttg caaaactgct tatcgcctcc ggtgcacaag tagatgcgct tgacggagta
     2401 acttccggca atactcctct tcagatgcta agcagcatta caggaaatca ttcttatatg
     2461 ctggatatag gaacagttct cctagaagcc ggggcaaacc caaaagatgc aatctcaaac
     2521 gactggagca tgagcgcatt tgcgtgtgcg ttgttaaatg gccaaaccag gctagctgag
     2581 ctaatgtttc caaaagtgca gaggcagggt tttggctgga ttgcagggct tggaaatctc
     2641 gaacttgtaa agtcattttt tgcaactaga gactcggatg aatacaagaa atactccata
     2701 cattgggctc aagaagaaac cgatccagaa tttgatctca gcaaaaacaa atcatcactc
     2761 atagcagcct atcttgtttc gtgcataaac ggcaaaacag aagttgccga atttctgtta
     2821 gaaaaaggag ttgatgtcaa tgtacttgtg acgggaactg actttgcagg aatcggcggc
     2881 acaggtcttc actgggctgc ctatcatggt cattttgata ccgttaaatt tctggtctca
     2941 aacggagcag acatatatgc agaggacgac gcatatgctc ttactcccgc aggatgggct
     3001 ggaagaaacg accattacga gattgccgac tatctgcttg agttaagatg ctcttgaatt
     3061 tccctaaatg gtacacacat taatataaga acatatatta aaagctgatc aggggagtta
     3121 taattaccat tatgaaggaa agaagcttta tagacactta tacaataaag ggcgattggt
     3181 ggcatacact ggaagacggt aggattcagt gtgacctctg cccaagaaac tgcaggctca
     3241 aagacggcca gcagggtttt tgctacataa gagaatcaca gaatggcgag atggtattaa
     3301 ccagttatgg cagagcatct ggtttctgca tagacccgat tgagaaaaag ccacttaatc
     3361 atttctatcc cggctcgagc gtactttcat ttggtacagc cggatgcaac cttggatgca
     3421 gattctgtca aaactgggat atatccaaag caagagaaga tgacagactc acctcaccgg
     3481 catcacctga aaaaattgca aaagccgcaa aagaacatgg atgcaagagt atcgcattca
     3541 catataatga ccccgttata tttgcagaat acgccataga ttcatcttta gctgcgcatg
     3601 agctggatat aaaaaccgtt gcagtaaccg caggatatat aaccgaagaa gcgaggggag
     3661 atttttacaa aaatattgat gctgtaaatg tagacctcaa ggcatttacc caggagtttt
     3721 acggtaaaat ttgttttgcc gagttgcagc cggtgctgga tacattgatc tggcttaaaa
     3781 cagaaactaa agtgtggata gaagtaacaa cgcttctgat cccggatcat aacgattcga
     3841 atgaagagat aacaaagctc tctgaatggt ttttacaaaa tctcagcccc gatgttcccc
     3901 tgcattttac tgcgttccat ccggatttta agatgatgga cgtgccggca acacctcctg
     3961 aaacactaag cagggcaagg aacattgcaa aggaaattgg tataaatcat gtatatacag
     4021 gcaatgtgta tgatacagaa ggtcagtcaa catactgtca taagtgcaac aatattttga
     4081 ttgagagaaa ctggtatgag cttggaaagt ggaacctgga taaaacagga tgctgcaaca
     4141 attgtggaga gaagctttca ggccattttg ataaaaatcc cggagactgg ggatcgaaac
     4201 gacttccggt gtttatacaa tgaaagacat tcgtccatat ttagagcaaa gatggtatcc
     4261 cagagatcct gatgaactca gtatcagtat agaaaactat cttaatactg cagagagaga
     4321 ccaaatagaa tccaaaatat acggcattat tgttcctcat gcaggacaca tatactccgg
     4381 tcaggtggct gcctatgcgt ttaattgcat aaaaggcgtg aagcctgaaa ctgttgtaat
     4441 tgtatctcct tcacattaca taggcggtgc tgacattatc gtttcccccc atacatcata
     4501 tagcactcca cttggaaata tagatataga cacacactta ctggatgata ttctaaaacg
     4561 tctatataaa gagtttgata taacaatatt aaaataccct gaggaccccg agcattctat
     4621 tgaagttgaa ctaccattcc tgcaagtgat ctatgaagat ttcaaattaa taccgattat
     4681 gataagaaat caggatgaga aagtatctaa ggctctggga cacgtacttg ctgatgtact
     4741 aaaagataaa atttcaataa taatagcaag ctccgatcta tcacattact acaatcaaaa
     4801 aacagctaac aagatggaca gagaaataat aaataggata gaatccttag accctgagtc
     4861 agttctggat gcagaagagc agggcattgg acaggcatgc ggcattggag ccattgccac
     4921 tgtgttgtgg gcttctaaag agatgggggc aaagaaagca cagattctta actacgcaac
     4981 atctggtgat gtttcaggtg actatgataa agtggtcgga tatgtttctg cagcactgta
     5041 ttaaacagta aaatacaacc aattagtgat tacaaataag ctcaccataa tttaggtata
     5101 agtctgtatt taactttcga tacatattcc atatagcccg gaagctcaga ctgaagcgtt
     5161 ttatcttcca aagacgtacg aattacaatc aaaacaactg ataaaactgc agggacaaaa
     5221 gcccacagcg atccaagaat tagcgggatc gcaatccaca taaccgccca tcctacataa
     5281 cccggatgac gcacatgtga atatggccct tctgtaacta ccctatgcgc tctttctgac
     5341 tgtatacgca caagtttgga aaaccattta tttacagcca tagcccagag aatatacaaa
     5401 tttcctatca caaataagaa tactcccagg taaagccaca ctactcctga aagctcagac
     5461 catttgtttt tttcatcaag acctgcaact actaatgttc ccaaaataaa gatcgatcct
     5521 atagacatca agatcatatc ccacttcttt gcatccctgt gaaatctgga acgctcttct
     5581 atgacttccg gatttacttt caaaagaatt acgagattta caacaataca gataaaaaaa
     5641 gtaaatatga aaatccagcc cctttcccaa ttaaaagtgc ccgcaaatcc aaaaagtaga
     5701 cctccaagga taaaaaaaga tagaaccatc caaactatgc tttttacaat caattgattt
     5761 aattgaagat catcttccga tctttcttct tttttatcat tcataacttt attccaacaa
     5821 agtaatttac agtacaatag tttctaatca ttaaaataag gctttattgt tagctattca
     5881 agttattatc ttgattttat tatgactggt ttgtaaaaca tattttattg tatgacaatt
     5941 tgacacaata cagttgtagt tttgatcgaa cacataagcc ccttaaatca ttaaataact
     6001 tatgagtcat attggcacac attttgatat atataataaa taagactaac agcagggggt
     6061 ttataaaaat gagtataagt tcattttgta ataaagaggt tatatgtgta gaaatgggaa
     6121 ccagtattga gcaagtcgga aacttgatgg aagaaaaaaa tattggttgc gtggtagtta
     6181 ttgatagtgg aaaaccttgc gggcttgtga cagacaggga tatactcata cgggtaatta
     6241 atagaggact cagtccggca gaaacatacg ttgacgatgt aatgagtgag ctagttatga
     6301 ctttagatga agatatgggc cttcatgaag cacttgaaaa agttaaggga atgagtgtaa
     6361 gaagatttcc tgtaacagat aagagcggga atttgaaagg aagtgtgact ctcgatgata
     6421 taatttatct tctgggaaaa gaaatgtatg atgttgcaag tataattaag agcgagagcg
     6481 ccgaacttta aacaaattat agctggttgt gtaaaaggga tagttattat taatataggg
     6541 aaagatattt ctaatttcta actaaaacaa aaatcctatt gctagagcta taactaaaac
     6601 agaacaatgc aaaacggaat agttgtaaga cctcttgagc aactgctgcc ccatgagcag
     6661 gaaattgaga tagtagaaag aaagggaata ggccatccgg atacaatctg tgacagggct
     6721 gcagaacagg tatctattgc cctttgcaga ctgtacttat cagaatttgg caaaattatg
     6781 catcataacg tagataaggc tcttcttgta ggaggatcaa ccaatcctgt atacagagga
     6841 ggaaaggttc taaaacccat agaaataata atcgctggca gagctactat ggaaaaaaac
     6901 gggaaggttc tgcctgtgga agaagttgcc agggaggcaa tattaaatta tttaagccgg
     6961 acaataagaa atttgaatgt tgaaaacagc attgagctaa aaataaagat aagacccgga
     7021 agttctgagc taaccgagct tttcagcagg ttcggtcctg agcaggttcc attttccaac
     7081 gatacttcta ttggcacggg gttttatcca ttcgatgaga ctgaggaggt tgtttacagc
     7141 atcgaaaaat ttctaaacag ccataaaata aagaaaaaat acccttttat tggagaagat
     7201 ataaaggtta tgggggtaag ggagcatgat aatataaaaa taacccttgc agtagccact
     7261 gtagacaagt acataaacga cctaaacgat tatatagata aaataaaaca aataagagaa
     7321 ttaattaaag aacaggattg gtttaaaaat agttatgaaa tttatataaa cacggcagat
     7381 agttatgaac aagagagtat ttatttaaca gtaacaggca cgagtggaga acatggtgat
     7441 gacggccagg ttggtagggg aaacagggca aatgggctta ttactccata cagacctatg
     7501 acccttgagg cggttgccgg taaaaatccc ataaatcatg tgggcaaact ttataacctg
     7561 tttgcaaacg acttatgcaa aacaatagtg gataacggct atgccgagga ggcttatgta
     7621 agtctcgtat ctcaaatagg taagccacta aacgaacctc aaatagttga tattaaggtt
     7681 aaagaacaga aagttgaaaa acccataatt gaaagcactg ccatagatat gctgggtgag
     7741 atgccgcaga tgtggaaaaa aattattgat ggcatgtatg aaatagcata aaagttatat
     7801 ttaattaact tttatttcat acaacatata atataagtat ggaacatgaa cacacaagcg
     7861 atcacgcctg ctgccatact ccgggtgaca agaaaagtat agattatctt ttctggggtt
     7921 ctctaataat agtagtgatc ctctgttcta tatattttct cttttattct gtagtagaaa
     7981 atgtaccgtg gttaaataca ctcagcagtt cagtactgga gctgttaaaa accatttggt
     8041 ggggaatttt acttggtgcg ctttctgtag ggcttctatc aaaaattccc cgcgagttta
     8101 ttatatccat actgggaaac ggaggaacac tcaaaggaat tcttagggct actcttggtg
     8161 gagtattttt agatctttgc agccatggga ttttaatggt gggagcaaaa ctctacgagc
     8221 gtggtgcaag catcgggcag gtgatgtcat ttcttgttgc aagcccctgg aactcttttt
     8281 ccctgacatt tattttaata gcactcattg gtcttaaatg gactctgtcc tttattgtac
     8341 tatccatgat aattgctgtt attagcggat atatctttga tactctggtg aataaacaaa
     8401 cccttcccgc aaacccaaac cgtatcgaaa tttctaaaga ttttaatttt ctgtctgaag
     8461 caaaaaagag ttttaataat acaaagtttg attcaaaact atttctagaa ataatcaaaa
     8521 acggcatact ggaatcaaga atggttgtga ggtggatact gtttggtgtg ctgcttgcat
     8581 ctgccattag agcatttgta ccaactgagc actttgtgca ttattttggc ccgacattat
     8641 taggactcgg attgacttta gtagctgcaa ctataattga ggtttgctcg gagggttcca
     8701 caccaatagc cgctgatctt cttaccaggg caggctcacc cggaaacagc tttgcgtttc
     8761 ttatggcagg tgttgctacc gattacaccg aattagcggt tattaaacag actactcaat
     8821 cgtggaagat tacatttctg cttccaatac taactattcc acaagtattt gtagtggcat
     8881 attttctaaa cagttcagtt cagtaaggcg ccactttttc tgtatcgtta tatatttcta
     8941 attcaaaccc atcagggtct aaaaagtgat aggccaccgg atcacctttg gagtttttct
     9001 tgatctctat aaatttaacg cgcttggatt ccagatttgt ttttgccttt tctatatcac
     9061 caacctcaaa tgcaatatgt gaaactgagt tatctgtatt tattccagtt tgcttagtag
     9121 aattctctgt cagtgctata agcgatttat cttttgtgcc taaaaatgat ttcttgtccc
     9181 cgccttcgct tctcccgtca tgcaggatta caagctccaa
//
LOCUS       DBNGSV010000380         6923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_622673, whole genome shotgun sequence.
ACCESSION   DBNGSV010000380 DBNGSV010000000
VERSION     DBNGSV010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_622673"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..660
                     /locus_tag="AC159U_13575"
     CDS             124..660
                     /locus_tag="AC159U_13575"
                     /EC_number="3.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF020965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HIR3338124.1"
                     /translation="MNKYIFILLVSVIFIYSPDPANSEDIEYRTVGLRLGTSNYNERD
                     FRSLEIDTNIGLPLTYKIYSSPNLYIKPKLNFSAGGLKQKKDTGYFFTLSGGLALFAI
                     DNKLVFDLAGGGALVTEDDIGEHNFGGPFQFAAHGGLAYKLTEKFLVGYRFYHLSDAG
                     IFDGRGLNRHLLELAFYF"
     gene            complement(665..1882)
                     /locus_tag="AC159U_13580"
     CDS             complement(665..1882)
                     /locus_tag="AC159U_13580"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3338125.1"
                     /translation="MSIKLNDIQQARHIIKDRVLNTPCSVSLALSKLTGTETILKFEN
                     KQFTGSFKDRGALVKLLSLDDKTKKQGVIAMSAGNHAQAVAYHSKSLDIPATIVMPIN
                     TPNVKVRKTQSFGAEVVLHGKDLFEAGQYTKKLMSEKNLTLIHPYNDEKIISGQGTIA
                     LEMLEDYPDLDVLVVPIGGGGLISGNAIAAKAIKQDIKIIGVQTENYPSMYKAVKAEE
                     AVFQSSSIADGIAVKTPGSLTLEIIRDLVDEIVLVSEIEIEEAVLTLLEIEKSVVEGA
                     GAVGLAAMLQDKEKFSDKKTGLILSGGNIDLLVLSSIIQRGLARTHRLARLKIIIPDV
                     PGSLSDVTELLGNSNANIIEVRHQRAFTNLSLKSAEVEFVIQTLGTDHLEEIIKTIKD
                     ADYDVHLEDIEVF"
     gene            1969..2328
                     /locus_tag="AC159U_13585"
     CDS             1969..2328
                     /locus_tag="AC159U_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338126.1"
                     /translation="MAEDFDTEIQNRVTAIFSDIVKPLTDELISDFNGVDGIQAELVS
                     DIPVIMGIKQYKSIMFKLPTGVEHVICVYWVDGGSEIVAENIRMVTLNRSFDIFDLDV
                     DELKKTIKVLAGLGKHV"
     gene            2347..3144
                     /locus_tag="AC159U_13590"
     CDS             2347..3144
                     /locus_tag="AC159U_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3338127.1"
                     /translation="MLTNFQWLSLITILLVTFAGGFYPLFRQQKEQQSIGVSFGQAFT
                     AGVFLALSLTLMLPSSLHLLGKAFPKVDYPIATVIVIGAFLFLLGLEHFTRHIHKKSN
                     SNVDEPAPAGIPIIMTVMIAIPSFFLGTALGVSETSAALFIWIAIMAHKSSAAFALAL
                     KMVRSTLKKHQTYIIFSLFALSTPVGIVVGQDIHQYLSESTMIIVKGTILALAAGTFL
                     YMSTLHEHEDAPMIKSCKNKRGFVIMLSGFVITAIVRLLIGAAHHMK"
     gene            3227..3730
                     /locus_tag="AC159U_13595"
     CDS             3227..3730
                     /locus_tag="AC159U_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338128.1"
                     /translation="MIKSKTSISIILLLLFALLAGYSTQVIAGSAGPYASGHGNLVEE
                     GDLRTFSFHARELKNSKVIGSLVLKNRSVGVRVKAKLDCLEINGNQATMSGIITQIMG
                     EGLDSLLGDEVWFRVEDNGEGFRNTGDRMTLLIVEVDEPPDPDECTEDLGLGLMNIRA
                     GNIQVNP"
     gene            3908..5305
                     /gene="gad"
                     /locus_tag="AC159U_13600"
     CDS             3908..5305
                     /gene="gad"
                     /locus_tag="AC159U_13600"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - GABA shunt [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HIR3338129.1"
                     /translation="MQKQKKLGDSKQSPVLTPLYTRDPLTSNIPKYEIPKGEMLPDTA
                     YNLIKDELMLDGNARLNLATFVTTWMEPEVTKLLTETFEKNMIDKDEYPQTAEIEMRC
                     VNIISRLWNSPENEIATGTSTIGSSEASMLGGMALKWKWREKMRAAGKPTDKPNMVMG
                     INVQICWDKFCTFWDIEPRFVPMEKGRYIMDAEQAAALCDENTIGVVAIMGSTFEGSY
                     EPVKEINDALDKLQKEKGLDIPIHVDGASGGFVAPFLQPDLEWDFRVPRVQSINTSGH
                     KYGLVYPGIGWIIWRTEEALPEELIFRVNYLGGDMPTFAINFSRPGNYVVAQYYNFLR
                     LGFEGYRRIHQASQDVALYLSAEIAKLGPFDLLSDGSDIPVFAFKLKDKVLKSGNYTV
                     FDLSDRLRQRGWQVPAYTFPKNCDNIAALRVVVKEGFSRDLADMLLEDLRRHIEWFDA
                     NPSHKPTECRTCFGH"
     gene            5371..6348
                     /locus_tag="AC159U_13605"
     CDS             5371..6348
                     /locus_tag="AC159U_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3338130.1"
                     /translation="MVYLTRLKKDAPEKGTGIRFAIYQAERPVGTIEAVNENILMLEE
                     AVKEAKQFGAHLISFPELYLQGYTMTPDVIKELAETVDGLSITRVRAIAEENEIGIIC
                     PYAEKDESSGKTLYYDSIAVIDSDGTLLHNYRKTHLFGQAERNNYSFGYCDTNEDPYI
                     VCEINDFPIGVLNCYEAEFFELSRILALKGAKLIAIPTAADYYYNLPDGKRTTVPYPD
                     ISTNLIPAHSLENHCFISYCNRSGSETVGSGTWQYRGNSIICGPHGDTLIAARTEDTL
                     MVADIIPGDYCATHPEGAYLKNRRPELYKQLVEMEGAFEGGYKYKTPPE"
     gene            6436..>6923
                     /locus_tag="AC159U_13610"
     CDS             6436..>6923
                     /locus_tag="AC159U_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338131.1"
                     /translation="MSNQSKMTPHLKKNIFGGVVGNILEWYDFAVFGFFAPIIGSQFF
                     PSDNPMAALIKTFGVFAAGYLMRPIGGIIFGHFGDKLGRKRALQLSVMMMAIPTTLIA
                     FLPTHAQIGLLAPILLVLIRLIQGVSVGGELVGSISFITEIAPKNRRGFFGSFSVCSA
                     VGG"
BASE COUNT         2037 a         1346 c         1470 g         2070 t
ORIGIN      
        1 catgaatacc gctttatcat tgctctttat catatatact actcctaaca acgtctaatg
       61 ccctaatcta ttataatatt actacttaag cttcaacaat aatattataa gcaattaaac
      121 tccatgaata aatatatatt catattatta gttagtgtga ttttcattta ttcaccagat
      181 cctgcaaaca gtgaggatat tgagtataga acagtcggac tcagactagg aacctcaaac
      241 tataatgaga gggattttag atcccttgaa atcgatacaa atattgggct tccacttaca
      301 tataaaatct atagcagtcc taatctttat attaaaccaa aactcaattt ttctgcaggc
      361 ggccttaaac aaaagaagga cactggatac ttttttactc tttcaggtgg gcttgctctt
      421 tttgccatag ataacaagct tgtttttgat cttgcaggtg gaggagcgct tgttaccgaa
      481 gacgatatag gtgagcacaa ttttggagga ccttttcagt ttgcagcaca tggggggctg
      541 gcatataaac ttacagagaa atttctggta ggataccgat tttaccacct gtccgatgcc
      601 ggaatattcg atggcagggg gcttaacagg catcttttag aactggcttt ttatttttaa
      661 taactcaaaa tacttctata tcttccaggt gaacatcata gtcagcatcc ttgatagtct
      721 tgattatttc ttctaaatga tccgttccca gtgtctgtat tacaaattca acctcggcag
      781 atttaagtga tagattcgta aaggctcttt ggtgtctaac ttcaataata ttagcattgg
      841 agtttccaag aagctctgta acgtcggata aagaacccgg aacatcggga ataataattt
      901 ttagccttgc aagcctgtga gttcttgcaa gccccctctg tatgattgat gagagcacaa
      961 gaaggtctat attgccacct gaaagaatga gccccgtctt tttatcgcta aacttttcct
     1021 tatcctgcaa cattgctgca agtcctacag cccctgctcc ttctacaacg cttttttcta
     1081 tctctaaaag agttagtaca gcttcctcaa tctcgatttc acttacaagc actatttcat
     1141 caaccaaatc tctaatgatt tcgagtgtaa ggcttccggg agtttttacg gcaataccat
     1201 ctgcaatact acttgattgg aatacagctt cttctgcctt caccgcttta tacatagaag
     1261 gatagttttc agtctgcact cctattattt ttatatcttg ttttattgcc tttgcggcaa
     1321 ttgcattacc cgaaattaga ccgccacccc cgataggtac tacaagcaca tccaaatccg
     1381 gataatcctc aagcatttca agcgctatag tgccctgccc tgaaataatc ttctcatcat
     1441 tgtagggatg aataagtgtt aagttttttt ctgacattaa tttttttgta tactgccctg
     1501 cttcaaacaa atcttttcca tgcagaacaa cttcagcgcc aaaagactgt gtttttctaa
     1561 cttttacatt tggagtattt atgggcatca caattgttgc cggtatatcc aatgacttac
     1621 tatgataagc tactgcctga gcgtggtttc cggcagacat tgctatcaca ccctgcttct
     1681 tagtcttgtc atccagagaa agaagtttga ctagtgcccc tctatccttg aatgaccctg
     1741 taaactgctt gttttcgaat ttaagtatag tctcagtgcc cgtgagtttt gagagtgcaa
     1801 gcgaaacaga gcagggggta ttaagaaccc tgtctttaat gatatgtctt gcttgctgta
     1861 tgtcatttag ttttatgctc atttgatatt atgaattaat aaagatttta tttaacaaat
     1921 attatatagt tatattaata taatattttt aaactacgaa taattaatat ggcagaagac
     1981 tttgatactg agatacaaaa cagggttacg gcaatattca gtgatattgt gaagccttta
     2041 acagatgagc tcatttctga ttttaatgga gtggatggca tacaagcaga attggtatca
     2101 gatattcctg tcataatggg tataaaacaa tataagtcca taatgtttaa acttccgacc
     2161 ggagtggagc atgtgatctg cgtttactgg gttgatggcg gaagcgaaat tgtagcagaa
     2221 aatattagga tggttacact caacagatcg tttgatatct ttgatcttga tgtggatgag
     2281 cttaagaaga ccattaaagt gcttgcaggt cttggtaagc atgtatgaat attttgataa
     2341 aaaacgatgc tgactaactt ccagtggtta tctcttatta ctattttact ggttactttt
     2401 gcaggtgggt tttatccact tttccggcag caaaaggagc agcagtcaat tggtgtttcc
     2461 tttggacagg cttttacagc aggggttttt ttggccctgt cacttactct tatgcttccc
     2521 tcatcgcttc atttgttagg taaggcattc cctaaagtgg attatcctat agctacagta
     2581 attgtgatag gtgcattttt gttcttactt ggtctggaac attttacaag acacattcac
     2641 aaaaagagca acagtaatgt agacgagcca gcgcctgcag gtattcctat aattatgaca
     2701 gtgatgattg ccattccatc attctttctt ggaacagcac taggcgtaag tgaaacatct
     2761 gctgcattat ttatatggat agccataatg gctcataaat catcggctgc atttgctctt
     2821 gctctaaaga tggttcggag cacactcaaa aagcatcaga cttatattat ttttagtcta
     2881 tttgcactct cgacacctgt tgggattgtt gttggtcagg atatacatca atatctttca
     2941 gaaagcacca tgataatagt taaggggacg attctggctc ttgccgctgg cactttttta
     3001 tacatgagta cactgcatga acatgaagat gcacctatga ttaaaagctg taaaaataaa
     3061 agagggttcg ttataatgct cagcggtttt gttattaccg ctattgttcg tctgctcatc
     3121 ggtgcagcac atcatatgaa ataactagta atatctatca ttgacattcc cggcaatttt
     3181 ttatataaga aaagtaagta ataaatatag tggggtgagg tgtctaatga taaaaagtaa
     3241 aaccagcata tctatcatct tattgttgtt atttgctctc ttggcaggtt atagcaccca
     3301 ggtaatagcc ggcagcgccg gtccatatgc aagcggtcat ggcaacctgg tagaagaagg
     3361 tgatctcaga acgttttctt ttcatgcaag ggaattaaaa aacagcaagg ttattggaag
     3421 tcttgtatta aaaaaccgta gtgttggagt ccgggtcaaa gcaaaactgg actgccttga
     3481 gattaatgga aaccaggcaa caatgagtgg aattattacg cagataatgg gagaaggcct
     3541 cgacagtctt cttggcgacg aggtttggtt tcgagtagaa gacaatggag aggggtttag
     3601 aaacaccggt gatcgcatga ctcttttaat agtggaagta gatgagcctc ccgatccaga
     3661 tgaatgtacg gaggatctgg gattgggttt aatgaatatc cgtgcaggaa atattcaggt
     3721 aaatccttaa actaatttaa ataatctgac tactggatta ggtacatata tatccgttaa
     3781 tttcacaaat tattaagatt aaaatattta tttatccggt tatacagagg agggttatat
     3841 ttttatctca caggtgacca tagttacaac gtctctacca tagcaaataa aagacaggag
     3901 aacactaatg caaaaacaga aaaaactcgg cgattctaaa caaagtcccg ttttaacacc
     3961 gctttatact cgggaccctc tcacatcaaa tataccaaaa tacgaaatac ccaagggcga
     4021 aatgctgccc gacacagcat ataacctaat taaggacgag cttatgctag acggtaatgc
     4081 aaggctcaac ctagcaactt ttgttaccac atggatggag ccggaagtca ctaaacttct
     4141 caccgaaaca tttgagaaga atatgatcga caaggacgaa tatccccaga cagccgagat
     4201 agagatgcgc tgcgttaata taatttcgag actttggaac tcacctgaaa atgagattgc
     4261 aaccggcact tcgaccatag gttccagcga ggcttctatg cttgggggta tggctcttaa
     4321 gtggaaatgg agagagaaga tgagagcagc cggaaaacct acggacaaac cgaatatggt
     4381 aatgggtata aacgttcaga tatgctggga taaattttgt accttctggg acatcgagcc
     4441 gcggtttgta cccatggaaa aaggccggta tataatggac gccgagcagg cggcagctct
     4501 ttgcgatgag aacacgattg gtgtggtcgc tattatggga agtacttttg aagggagcta
     4561 tgagcctgta aaggaaataa acgacgcatt ggacaagctg caaaaggaaa aaggtcttga
     4621 tatccccatc catgttgacg gagcaagcgg cggttttgtt gccccgtttc tacagccaga
     4681 tctggagtgg gatttccgcg tgccccgtgt gcagtcaatt aatacctcag ggcacaagta
     4741 cggacttgtc tatccgggta taggatggat catttggcgt actgaagagg cacttcctga
     4801 agaactaata tttcgggtta actacctagg tggcgacatg ccgacttttg ccatcaactt
     4861 ctcacgtccc ggaaactacg tagtagcaca gtattacaac ttcctgcgtt taggatttga
     4921 gggctacaga cgcatccatc aggcaagcca ggacgttgct ctttatctct cggcagagat
     4981 agccaagctt gggccttttg atttgctaag cgacggtagc gatattcctg tttttgcatt
     5041 taaacttaag gataaagtgt taaagagcgg caattatacc gtgtttgatc tatctgatcg
     5101 tttgcggcaa cgaggttggc aggttccggc gtatactttt ccaaaaaact gtgataatat
     5161 agctgcccta cgtgttgtag taaaagaagg gttcagtcgc gaccttgccg atatgctgct
     5221 tgaagacctt aggcgtcaca tcgaatggtt cgatgcgaat cccagtcata aacccacaga
     5281 atgcaggact tgttttgggc attaattgtt ctaaagctgg taaaactttg ccctatcctg
     5341 aaaaatgaat agatagaagg aggagtaaat atggtctatt taactaggtt aaaaaaagat
     5401 gctcctgaaa agggaacggg cattcgcttt gccatatacc aggctgagag accagtaggt
     5461 accatagagg cagttaatga aaacatcctt atgctggaag aagctgttaa agaggctaaa
     5521 caatttggtg ctcatctcat aagctttccc gaattatatc tgcaaggcta cactatgact
     5581 cccgatgtga taaaggagct tgccgaaacc gttgacggcc tgtcgattac gcgagtaaga
     5641 gcgatcgcag aagaaaatga aattggcatc atttgccctt atgcagaaaa agatgaaagc
     5701 tctggaaaaa ccctctatta tgattcaatt gctgtgatag attctgatgg gacgctgcta
     5761 cacaattacc gaaagacgca tctgttcgga caagccgaga gaaataatta ttcatttgga
     5821 tattgtgata caaatgagga tccttatata gtctgtgaga taaatgattt tccaattggt
     5881 gtgctgaact gttacgaagc cgagtttttt gagctatcaa gaatactagc cttaaaaggt
     5941 gctaaactca tcgctatacc tacagctgcc gattattatt acaaccttcc agatggaaag
     6001 cgcacgaccg ttccttatcc tgatatttca acaaacctta ttccggctca ctcgttagaa
     6061 aatcactgct ttatctctta ctgtaacaga tctggatcag aaacggttgg atcgggtact
     6121 tggcagtaca gaggaaactc tattatttgt gggcctcacg gcgatacttt aatagccgca
     6181 aggactgaag atacgctcat ggtagcagat attatccccg gcgactactg tgctacacat
     6241 cctgaggggg catatctcaa gaaccgcaga ccggagcttt ataaacaatt ggttgagatg
     6301 gaaggtgcgt ttgaaggcgg ttataagtac aagacgccgc cggaatgatc tattataatg
     6361 gatcaaggtt caaagatgaa aagttattga ttcaaatttc tattaagcct ataatgaaaa
     6421 ttattggagg taattatgag caatcaatca aaaatgacac ctcatcttaa gaaaaatata
     6481 ttcgggggag ttgtcggaaa catccttgaa tggtacgact ttgcggtgtt tgggtttttt
     6541 gctcctataa taggctcgca attcttccct tctgataatc cgatggcagc actcataaaa
     6601 acttttgggg tttttgccgc aggttatctg atgcgcccaa taggaggaat aatctttgga
     6661 cactttggag acaagcttgg cagaaagcgg gccctgcagc tctctgtgat gatgatggca
     6721 attccaacta cactcatagc ttttcttccc acacatgcgc agattgggct tttggcacca
     6781 atattactcg tattgatacg acttattcag ggagtatctg taggtggaga acttgtaggt
     6841 tcaatttctt tcataaccga gatcgcccct aaaaacagac gcggtttttt tggcagtttt
     6901 tctgtatgca gcgcagtcgg agg
//
LOCUS       DBNGSV010000381         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_276450, whole genome shotgun sequence.
ACCESSION   DBNGSV010000381 DBNGSV010000000
VERSION     DBNGSV010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_276450"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="AC159U_13615"
     CDS             complement(<1..532)
                     /locus_tag="AC159U_13615"
                     /inference="COORDINATES: protein motif:HMM:NF039593.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3338132.1"
                     /translation="MKFKNNKAVVGLILIIITGLALFGSKIPVVNKIPLFTQKHEHEY
                     MPVMDEDGAIEYWTCAMHPSVRLDEPGQCPICGMDLTSVEKPIKHKSSDMDNASSQNM
                     HDHSGHGTGVMPKKQEDGQVSSEFIVSPQRQQMIGVKTEIVSIRSMNRTIRTVGKVEL
                     DETKIEHIHTKVSGWID"
     gene            complement(534..1817)
                     /locus_tag="AC159U_13620"
     CDS             complement(534..1817)
                     /locus_tag="AC159U_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3338133.1"
                     /translation="MYFYLILTLFLFINSAVLEAKESFSLDSAIRQALDNNPEIKAAQ
                     AMSLASAKRPTQVSSLPNPTIGLRFKNVSFSEITLGEDPRTDIQAYFIQDIPFPTKLS
                     SKRKIAEQVSEAQKWKAESVTRRVIAELKKEYYNWYLISKSLEITKRNKELLEQFVKT
                     AEIKYEVGKGIQQDVIKAQVELSSFIHTVEHLSMKKEITEARIKKILNLSQHSDIGSP
                     EKIEKTDIDITLENLSEMTKNQSPQLLSELELVEGSKEQLQLAKKQYLPDFVIQGTYF
                     NRDGGTDNFEDLWQVGLGLKVPLYFWRKEKPAIQEAHLNLQASKDRYDDSTSEILFEI
                     KDKYISASTADRLMGLYKDGIIPQSQISLESAISGYQVGDVDFLTLLNNLITLFNFEI
                     EYYKQLAEHEISVAMLEQISGVDFNKTESGNQGRN"
BASE COUNT          521 a          412 c          332 g          659 t
ORIGIN      
        1 tatcgatcca cccgcttact tttgtgtgta tgtgttctat tttagtttca tcaagctcaa
       61 ccttaccaac tgttcgaatc gtcctgttca tagaccgaat agagactatc tctgtcttaa
      121 caccaatcat ctgctgcctt tgtgggctta ctataaactc tgaacttact tgtccatctt
      181 cttgtttttt gggcatcaca cctgtgccat gaccactatg gtcatgcata ttctgtgatg
      241 atgcgttatc catatcgctg ctcttatgtt ttattggttt ttcaacagat gttagatcca
      301 tcccgcatat agggcactga cccggttcat caagcctcac agacggatgc atggcgcatg
      361 tccagtactc tatagcccca tcctcatcca ttaccggcat atattcatgc tcgtgttttt
      421 gtgtaaataa tgggatttta tttacaacag gtatttttga tccaaacaag gctaatcctg
      481 taataataat taagattaac cctactacgg ctttattgtt tttaaatttc atctcaattt
      541 ctcccctgat taccagactc tgttttatta aaatctactc ccgatatctg ctcaagcatt
      601 gctacagata tctcatgctc ggcaagctgt ttgtagtact caatctcaaa attaaatagg
      661 gttatgagat tattaagtag tgtaagaaaa tccacatcac ccacctgata gccggaaatt
      721 gcagactcaa gggaaatctg tgactgaggt atgattccat ctttatacaa gcccatgagc
      781 ctgtctgcag tactagcaga aatatattta tccttgatct caaacaaaat ctctgacgta
      841 ctatcatcat atctatcctt actagcctga aggtttaaat gagcctcttg tattgcaggc
      901 ttttcttttc tccagaaata aagcggcact ttcaggccaa gccccacctg ccagaggtct
      961 tcgaaattat cagttcctcc gtctctgtta aaataagtgc cctgaatcac aaaatccgga
     1021 agatactgct tttttgcaag ctgcagctgt tcttttgaac cctcaacaag ctcgagttct
     1081 gaaagtaatt gtggggactg attcttggtc atttcgctta gattttccag tgtgatgtca
     1141 atatccgttt tctctatttt ttcaggacta ccgatatcag aatgctggga gagatttaat
     1201 atcttcttta ttcttgcctc ggtgatctct tttttcatac tcaggtgttc tactgtatga
     1261 atgaacgaag agagttccac ttgtgcttta ataacgtcct gctgtatacc ctttccgact
     1321 tcgtatttta tctctgctgt ttttacaaat tgctccagaa gttccttatt tcgttttgta
     1381 atttcgagtg atttactaat cagataccag ttgtagtact cttttttgag ctctgctata
     1441 actcttcttg taacagactc tgctttccat ttttgggcct ctgatacctg ctcggcaatt
     1501 tttcttttgg aagagagttt ggttggaaat ggtatatcct gaataaaata tgcctgaata
     1561 tcagttcttg gatcttcacc aagcgtaatc tctgagaaac ttacattttt aaacctgagt
     1621 ccaattgttg gatttggaag tgaagacacc tgagtaggtc tcttagcaga agctaaagac
     1681 attgcttgtg cagcctttat ttcaggatta ttgtccaaag cctgtctgat tgcgctatcg
     1741 agactaaaac tctcctttgc ctcaagcacg gcagaattta taaataaaaa cagtgttaaa
     1801 ataagataaa aatacatatt tccctccacg gttaagaatt agagttccta aatgaaccca
     1861 agtggataaa acttccctgt aaacgggaaa aaataacgga gagaaatatt tctagattaa
     1921 ataa
//
LOCUS       DBNGSV010000382        15973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_414540, whole genome shotgun sequence.
ACCESSION   DBNGSV010000382 DBNGSV010000000
VERSION     DBNGSV010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15973
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_414540"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..503
                     /locus_tag="AC159U_13625"
     CDS             96..503
                     /locus_tag="AC159U_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338134.1"
                     /translation="MTDENKRSLVQVNKFQKLIIVPIIIFCAIAGIMVYLTLEYNIRI
                     SINPKEATILSIDALQFKEAVPYMIISIAAMLICIVGWTYYITNRIVGPHNRIIKELD
                     DLIDGSKEGPITVRKDDKMFSELVSRINKLAGL"
     gene            657..1550
                     /locus_tag="AC159U_13630"
     CDS             657..1550
                     /locus_tag="AC159U_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3338135.1"
                     /translation="MKFASIDIGSNAIRLLLTKVFENGSGPVFKKDSLVRMPIRLGKD
                     VFTTKKVSKEKIDNLVNTMIAFKYLIDAYKPIDYMACATSAMREAENGSEIVKLIKKK
                     SGIEVEIIDGKREAEIIYENHIAERLNPKENYLYIDVGGGSTELTLISNTEIINSQSF
                     DIGTVRILEDKVTKKHWQEMQRWLEQNTSSYKSISGIGSGGNINKMFKMALKGGDDPL
                     TYKNIKNICKELKTYTYEQRITKLGLRPDRADVIIPAAKIYISVMKWAGIKKLYVPQI
                     GLSDGIIHVLYEEQKRKKLII"
     gene            1516..3069
                     /locus_tag="AC159U_13635"
     CDS             1516..3069
                     /locus_tag="AC159U_13635"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR3338136.1"
                     /translation="MRNRKEKNLLFEIPEDIDEKSIIAELSNIYQVVKDSYKTNRQIY
                     LDTFDWRLYNQNLILIKESSNYLLKNKNTLNDVYSHETDSAHNPKFWWDFNDSLLKEE
                     LKKIIDLRALVQIISIRKTIQSYKLLNKDDKTVVILYFETIKPVGRNGALQTTNLLIC
                     KPLRGYEKDLKQIKQPIVNLGLTLSKTSYYELLLSKSAIVPADYSSKLNICLTPDMSC
                     RDALLTILTDLLGTIKKNEEGIKMDIDTEFLHDFRVAVRRTRSALSLIKGVFDKEQLR
                     IHRSNITKLGTATNALRDLDVYLLKENQYKKLIPRAQHKGLELIFDKLKEQRKSELVK
                     LKRFLNSKHYKKIISDWENFLNTGGQINDETENAGTNVLSTAKRIINKKHKEILKAGI
                     IINDTSPDEQLHSLRIECKKLRYMIEFFSSLFPKNEINQFIKQLKKLQDNLGDFNDLY
                     VQQQSLKEYSEKFDSHNKDITISIGGLIAVLYRNQNETRKQFNSSFEQFASKENTSLF
                     NKMFRDKKS"
     gene            3073..3810
                     /locus_tag="AC159U_13640"
     CDS             3073..3810
                     /locus_tag="AC159U_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3338137.1"
                     /translation="MIISLYSMKGGVGKTSAAVNLAYLSAKEGFNTLLCDLDPQGAST
                     YYFRIKASKDFNTKKLLKGGKNLDGNIKATDYENLDLLPSKLSLRNMDLELDSHKNSK
                     TRFNSIVKELSKEYEIIFMDCPPGITLLSENIFNSSDMILVPLIPTTLSIRTYEKLIK
                     FFKKEKLDRSKIYAFFSMVEIRKSMHRDTITKMSQEIKNLLYSQIPYRSNVEKMGIYR
                     KPVNDFEPFSPAAFAYSTLWNEIKEVE"
     gene            3912..6587
                     /locus_tag="AC159U_13645"
     CDS             3912..6587
                     /locus_tag="AC159U_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3338138.1"
                     /translation="MNSLDQTIRNLPFLKRLGKEKVNKLLEQAQIVEFKKDQVISKQF
                     EHSHSFYFLLDGEVNFSIRVENNTDVFSVGKSDEKFTPIGWSGFRVPGRYATTVTCET
                     HCTLLKWSFDDLMTLFEEDPLIGKKFLCFVLQKAVHLLNQVWIELTKFNNSDWYVDFG
                     QETESPDEQEDISVQSPIELLRQSPFFEIFSEQDLYKLSEIAEKKYYYGGDRVFTQGE
                     QASGIDLLVSGRTAMCFSPEATTKPLDDDQVEDSASLRLVQKTGYVVGWAGAFDGFVN
                     DCTAVATRNAVIYHFKKTSLDKLFKDNSVLALNFVKRLLWLLSNQLRNARARLISQHY
                     ELEITSIRNLMEQNAAQLSVTSALHKVAHLLTHTYTIGDAFNLLFQVEKDGSTLEKGL
                     ARLCLDILGKVYKEHLFFEGLKKVYQFVSEAPDNMTTKGVRVQSSRKYVELFKQIPHV
                     VEGWENLPEKAGNIFISNHLVNDPYNTLPNNFQITLDSHFISSMILYKKYGDPGVRVV
                     RVPRSEEFGHQNYYNKLGHINVYTKESDSSEESLRIQKEQNKLFYKTATEYIKSGQNL
                     MLSPEGTSYRTEDSPGPFKPGAFRLAASIDPEPFIVPIVVANFDKRLNRNVFSLIIKE
                     PFKLSDLVDKPQENRDGLAQFLDELQNKYKGDVQEAIALANKTASTKINLKLFERVKE
                     DSRVYSKTNMTIDELMFEGNVKLLERRRVGTKENPVVFYGSSSLRLWETIEQDFPGHD
                     ILNLAFGGSTIDYCSYYFDRLIKPSNIKSFVFYAGDNDIGHGKSATQVFDSFLILYNK
                     FRESFTDTKFTFVSIKPSIERAKFINRIQKANKLIKEFLEREPNTFYLNVHDQMLNGD
                     KLAKPELYTEDELHMSEKGYELWKEVFLENADEIF"
     gene            complement(6584..7114)
                     /locus_tag="AC159U_13650"
     CDS             complement(6584..7114)
                     /locus_tag="AC159U_13650"
                     /inference="COORDINATES: protein motif:HMM:NF053499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbD-related protein"
                     /protein_id="HIR3338139.1"
                     /translation="MSTLKYELKIIFKTTIVVIIILLLFLTFGGKAHKYYDYNHARYE
                     GLFERGWVPEYIPESSKRIEIVNGASYSAACVRFYIPASHSDDFVRILNQKGYKQVND
                     SYEEPPYFGLLYNRCAFDVDDVKNSSLEIYNLKTETLSTGNYVIGRHDTYFALDKYSG
                     VVYYWLVPRSSSSDNL"
     gene            complement(7125..7739)
                     /locus_tag="AC159U_13655"
     CDS             complement(7125..7739)
                     /locus_tag="AC159U_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR3338140.1"
                     /translation="MAEDSSYKVPSKTVTVEQVIKNSKFITSVTRVTDKQKAKEFIDT
                     VSKKYSDASHNCYAFVAGNPDSTTDIGFGDDGEVSGTAGKPILSVLQHKKIGEIVVVV
                     SRYFGGTKLGTGGLVRAYTSSVQDALDKLPLEDCVALKSITLKFDYQYENTVRQVLNK
                     FSLEIADSIYTDNVDMKLEVPEDNLNQLETELQNSTSGKVNILY"
     gene            complement(7755..8108)
                     /locus_tag="AC159U_13660"
     CDS             complement(7755..8108)
                     /locus_tag="AC159U_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3338141.1"
                     /translation="MGSDEIDLEYYRDKLAHIKSELLKEGEAGQEATKTVELDQSKVG
                     RLSRMDAMQSQAIAVESQRRREIRIQRIDAALKRIDEDDFGYCTHCGLEIDPKRLEFD
                     PSALLCIDCARSGEG"
     gene            complement(8118..8321)
                     /locus_tag="AC159U_13665"
     CDS             complement(8118..8321)
                     /locus_tag="AC159U_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase family protein"
                     /protein_id="HIR3338142.1"
                     /translation="MPYVNIKITREGATKEQKAKLIEGATNLLKEVLGKNPATTVVVI
                     DEVDTDNWGIAGEQVTERRKKGN"
     gene            complement(8379..9209)
                     /locus_tag="AC159U_13670"
     CDS             complement(8379..9209)
                     /locus_tag="AC159U_13670"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR3338143.1"
                     /translation="MKKIIDLENPYKGINLKDYHIEKRRLQLELLNIQQDIVNKGKRM
                     CITFDGRDAAGKGSTIIRFTEKLMPKYYRIVELGIPTPKESKRWFHRYEKQLPKKGEI
                     VFFDRSWYNRALIEPTMGYCSQKQYEYFMGKVLDWEHKLIKDGLKLVKFYLSVDSEQQ
                     LIRFQERLADPLKFWKFSENDLYARKKWEKYTNYKNQMFQHTSSNKSPWITVNSNSKI
                     EARLNCMLYVVRLYGDKKFVPLTGVDVSNQYSVEVDGVLFEALNSLQYETLKQLKGRK
                     "
     gene            9350..10459
                     /locus_tag="AC159U_13675"
     CDS             9350..10459
                     /locus_tag="AC159U_13675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3338144.1"
                     /translation="MKEYDLIIVGGSFSGLSCAKSASARGLQTVLFDKKRAPDASTQS
                     TGIFVKEIADQIDLPSVLTRKIHGVRLYSPNLNSIDLVSPGYYFLATNTTQVLKWLAD
                     QAADTGAELLYGQNITDAYIKGNKIVLPNQQVTTSYLVGADGVRSRVAKIFGLGQNTQ
                     VLLGIEYEVEGLTNLDGDFLHVFLDSELAPGYIAWIVPGVHFSQVGLASYYPKKPDIK
                     QFYQIVENKFGGKNTKIVSRRGGFIPCGGTVSPLSKNNVCLLGDAAGMVSPMTAGGIH
                     PAIEVGRLLGIAISDYLQDKGFAPEKEIAKHIPSYLFKNGLRRFFNFLTPPNFLLNIL
                     IDNPLFRKSAQIIFFHHRGLLSKKAWAEILGKEKK"
     gene            complement(10537..11061)
                     /locus_tag="AC159U_13680"
     CDS             complement(10537..11061)
                     /locus_tag="AC159U_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3338145.1"
                     /translation="MAKIPYVEKEQAAENLQPVYDGLQQKFGVVPNVIKAMANAPEML
                     SGFMPFLGAALGQTQVSNDLKELAILTTSKLNGCSYCTAHHTAAGKRAGLTDEKIAAS
                     ADATSAALDDKEKAIVQYTTELAQNVAASEESLAQMRNHFNDGEIAELTMVAGTFHVL
                     TRFADTFKVDLEHG"
     gene            complement(11119..11697)
                     /locus_tag="AC159U_13685"
     CDS             complement(11119..11697)
                     /locus_tag="AC159U_13685"
                     /inference="COORDINATES: protein motif:HMM:NF028235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3338146.1"
                     /translation="MGRHYEFNKDETLSKAMRVFWQKGYKATSMKDLVDEMGIQPGSI
                     YNTFGDKHSLFLESIKHYGEVVTANTIKILNEGESPTENIKKFFNEMIQRPYDKRCIG
                     CFVVNSVVELAPHDDQTADIVNGILKNIESAFYECLLKAQQLGEISKESNVKALSSFF
                     ASCTHGLLVTGKSDASGEQMKDIVNVMLSTLK"
     gene            complement(11876..12388)
                     /locus_tag="AC159U_13690"
     CDS             complement(11876..12388)
                     /locus_tag="AC159U_13690"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3338147.1"
                     /translation="MKRLSLSVFLLVLALSVSAFAAHHYKGKHYKWWENEEIATKVGL
                     SEKQIADLNAIHESYAGKKAELWAEIKTIKKELYDLMGDPKATDEAITAKHNEKIAKK
                     TEKMQLKLEMKLKMRALLSDEQIVTLSGIMKEKWEQKWDGKDCGHKECGDKECSYKDK
                     KEDCPYKDKK"
     gene            12523..13428
                     /locus_tag="AC159U_13695"
     CDS             12523..13428
                     /locus_tag="AC159U_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3338148.1"
                     /translation="MENKQKFIAAAVQASPVFLNKTKTIAKVNKLIAEATKNGAELVV
                     FPEAFIPTYPFWPKDIGTPEGRKLIIDAYTDLHKNSVEVPSADTEKIGKAAKKAGVYV
                     VIGVNEKEGGTLYNTILYFGKDGSLLGKHRKFMSIDSEKCIWANGTAKDVTVVNTDIG
                     KVGGLFCYEHHMTLAKYAMFQKGEQIHVGLWGGHGFVKNTMDFASKQYAFEGQTFVII
                     SSMTITEDQIPDSFPLKKQTMWDYPGGSGIINPRGEYIAGPVYGKEEIVYGEIDTDLI
                     LRAKAVIDGTGHFSRPDIFQLEVKE"
     gene            13533..14159
                     /locus_tag="AC159U_13700"
     CDS             13533..14159
                     /locus_tag="AC159U_13700"
                     /inference="COORDINATES: protein motif:HMM:NF022763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HIR3338149.1"
                     /translation="MHIVKLLLFLNIIIGSNLVFAQDIPQAEFAKCSLTKNDIQRLSC
                     FDNLSKKYELNKPASKIVNKGNWAVQTDISPIDDSTNVYLAVPGDQAFKGWLKSYNPQ
                     LMLRCKENVTELLIHTGMAPDVEYGHRGATTTLRYDKEKAYKLRMSKSTDGEALFFPN
                     PISNIKKMMNHSTLLFQFTPYNASPTMTTFKISGLSEAAKPLREHCGW"
     gene            14283..14849
                     /locus_tag="AC159U_13705"
     CDS             14283..14849
                     /locus_tag="AC159U_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338150.1"
                     /translation="MKLKIVSLMFLYLSLYSIYLPTYAAETTTYGNWTVTKKVDEFTG
                     DKSLEAKIRSINIITGWLSQKGKAYISIHCGKLNYLTFISIGIEGGVGFNNSFMDFKV
                     DDNDGVQTFSWNGDIGDDIIFSAVGKGAIFSESSKTPIMFEQMKTGKKIKIRINPFKT
                     ERQVVEFNLNGSDKALTEVETFCTELNK"
     gene            complement(14880..15407)
                     /locus_tag="AC159U_13710"
     CDS             complement(14880..15407)
                     /locus_tag="AC159U_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3338151.1"
                     /translation="MKYVVAIIMGLFSGFLIYMGSAMLFSDVGSSTGPSGLFIAITFL
                     GGWIITSYFILKGAKSLSKVFKRGFLIGAAEWFAFIPIGFIVGGKAASSLETQSSAEA
                     AGAAIGGGIFAMLTGGLSIIMAVCCLIGFFIARHMGKEMKAEENDATKKCPECAENVK
                     MDAIKCRFCGADLSK"
BASE COUNT         5306 a         2990 c         2741 g         4936 t
ORIGIN      
        1 ccaaaattga acaacaacca tacaattcta aaaaaatgct tgaataataa acagtacatt
       61 ctataatctt ttgaaaaaga gtcataggta atacaatgac agacgaaaat aaaagaagtt
      121 tagttcaagt aaacaaattc caaaaactta tcatagttcc tataataata ttctgtgcta
      181 ttgcaggaat catggtctac cttactctgg aatacaacat acgaatcagt attaacccaa
      241 aagaagcaac tatcctcagc attgatgctt tacagtttaa agaggccgtt ccatatatga
      301 taatttctat tgcagctatg cttatatgca tcgttggctg gacttattat attactaata
      361 gaattgtagg cccccataac agaatcataa aggagcttga tgatctgata gatggcagca
      421 aagaaggtcc tatcactgta agaaaggatg ataaaatgtt ttctgagctt gttagcagaa
      481 tcaacaaact tgcaggcctg taaacaagat tctgtgaaat ttcatctatt gatattaata
      541 gtgaattttt taagcttata caacaagtta atattgtctt agttttttga cttttctttt
      601 aaaaatttaa attatttaga acttcatatc ccatattttt tctaaaatca atttaaatga
      661 aatttgcatc aattgatatt ggctccaacg ccataagact cctattaaca aaagtttttg
      721 aaaatggcag tggcccggtc tttaaaaaag actcgcttgt ccgcatgcct attcgtctcg
      781 gaaaagatgt gtttacaaca aaaaaggttt caaaagaaaa aatagataat ctggtaaaca
      841 ctatgattgc atttaagtat ctcattgatg catacaaacc aattgattat atggcctgtg
      901 ctacttctgc aatgagagaa gctgaaaatg ggtcagaaat agtaaagtta ataaagaaaa
      961 aatcgggaat tgaagttgaa attattgatg gtaaaagaga agctgaaata atttatgaaa
     1021 atcatatcgc agaacgatta aatccaaaag agaattactt gtatattgat gtaggtggtg
     1081 gaagtacaga gcttacttta atatctaata cagaaataat aaattctcag tcgttcgata
     1141 tcggtactgt tcgtatctta gaagataaag tcacaaaaaa acactggcag gaaatgcagc
     1201 ggtggctcga gcaaaatact tcttcataca aatcaatttc aggcataggc tccggtggaa
     1261 atattaataa aatgttcaag atggctctaa aaggaggaga tgatcccctc acatataaaa
     1321 acattaaaaa tatctgcaaa gaactaaaaa catatacata tgaacaaaga atcaccaagc
     1381 ttgggctaag acccgaccgt gcagatgtga taattccggc agctaaaatt tatatctctg
     1441 taatgaagtg ggctggcata aaaaaacttt atgttcctca gataggatta tctgatggta
     1501 tcatacatgt gttatatgag gaacagaaaa gaaaaaaact tattatttga aatcccggaa
     1561 gacatagatg aaaaatctat tattgctgag ttatcaaata tataccaggt tgttaaagac
     1621 tcatacaaaa cgaataggca aatctatctg gatacattcg actggcggct ttacaatcaa
     1681 aacctaatac tcataaaaga atccagcaac tacttactaa aaaataaaaa cacattaaat
     1741 gatgtttact ctcatgaaac agattccgct cacaacccca aattctggtg ggattttaat
     1801 gattcactac ttaaagaaga gcttaagaag attatagatt taagggcact cgtacaaata
     1861 atatcaatac gtaaaaccat acaatcatat aagttactta ataaagatga taagacagta
     1921 gtaattcttt actttgaaac aataaaacca gtcggaagaa atggcgcttt acaaacgaca
     1981 aaccttttaa tttgcaaacc acttagaggg tatgaaaaag accttaaaca gataaagcaa
     2041 ccaattgtta atcttggatt aacactttct aaaacttctt attatgagtt acttttgagc
     2101 aagtcagcga ttgtacctgc tgattactca tctaaactga atatctgttt aacccctgat
     2161 atgagctgta gggacgcact attaacaatt cttaccgatc tactaggcac gattaagaaa
     2221 aatgaagaag gtattaaaat ggatatagat acagagttcc tgcatgattt tagagtagcc
     2281 gtaagaagaa ccagatctgc tctctcgctc ataaaagggg tttttgataa agagcaactt
     2341 agaatccata gatcaaacat tactaaactt ggaacagcta caaatgctct aagagacctg
     2401 gatgtttacc ttttaaaaga aaatcaatat aaaaaattga tacccagggc acagcataaa
     2461 ggccttgaat taatttttga taagttaaaa gaacagagaa aatcagagct tgtaaaatta
     2521 aaaagatttt taaactcaaa acactataaa aagataattt ctgattggga gaatttttta
     2581 aacaccggcg gtcaaattaa tgatgaaaca gaaaacgcag gcacaaatgt attgtccaca
     2641 gcaaagagaa taattaataa gaaacacaaa gaaatactga aggcaggaat aataattaat
     2701 gacacgtcac ctgatgagca gcttcacagc cttagaattg aatgtaagaa actgcgctac
     2761 atgatagagt tcttctcttc attatttcca aaaaatgaaa taaaccagtt tataaaacag
     2821 ctcaaaaaac ttcaggataa tcttggtgat ttcaacgacc tgtatgtgca gcagcaaagc
     2881 cttaaggagt attcagagaa atttgattcg cataacaaag atattacaat ttccattggt
     2941 ggtttgatcg ctgtattata tcgaaatcaa aatgagacca gaaagcagtt caatagttcg
     3001 tttgaacagt ttgcatcaaa agaaaacacg agtttgttca ataaaatgtt tagagacaaa
     3061 aagagctgac aaatgattat ttcactttat agcatgaaag gtggagtcgg caaaacatct
     3121 gctgctgtaa atcttgcata tttatcagca aaagaaggtt tcaatacact tttatgtgat
     3181 ctggaccctc agggcgcatc cacctattac ttcagaataa aagcatccaa agactttaat
     3241 acaaaaaaat tattaaaggg cggcaaaaat ctggatggaa acatcaaggc aacagattat
     3301 gaaaatctcg atctgcttcc atctaaactc tcccttagaa atatggattt agagcttgat
     3361 agccacaaaa attccaagac aagatttaac agtatagtaa aagagcttag taaagaatat
     3421 gagattatat ttatggactg tccaccgggt attacacttc tttccgaaaa catattcaat
     3481 tcatcagata tgatattagt gccgcttatc cccactaccc tgtctattcg gacttatgaa
     3541 aagctgatta aatttttcaa aaaagaaaaa ctggacagat cgaaaatcta cgcttttttc
     3601 tccatggtag agataagaaa atctatgcac agagatacaa taaccaaaat gtctcaggag
     3661 ataaaaaact tactgtacag ccaaattcca tacaggtcta atgtagagaa aatgggaatt
     3721 tacagaaaac ccgtaaacga ttttgaacca ttttctccgg cagcgtttgc ctactccact
     3781 ctttggaatg aaatcaaaga ggtcgaatag tttttcttta tatccggcat tccagaaccc
     3841 aaaccttcta aacacttttt atttcccaaa taataattga attttcccaa tactaattaa
     3901 tatttagaaa tatgaattcg cttgaccaaa ctattcgaaa cttgcctttc cttaaaagac
     3961 tggggaagga aaaagtaaat aaacttttag agcaggctca aattgtagaa tttaaaaaag
     4021 atcaggttat ttcaaagcaa tttgagcata gccattcttt ttatttcctt ctagatggtg
     4081 aagtgaattt cagtataagg gtagaaaaca acaccgatgt attttcagta gggaaatcag
     4141 atgaaaagtt cactccaatc ggctggtcgg gttttcgtgt tccgggccgt tatgcgacta
     4201 ccgttacttg tgagacccac tgcactttac tcaagtggtc ctttgatgat ttaatgacac
     4261 tgtttgaaga ggatcccctt attgggaaga aattcttatg ctttgtactc cagaaggcag
     4321 ttcacctatt aaatcaagta tggatagagc ttaccaaatt taataattca gactggtatg
     4381 tagattttgg ccaggaaaca gaatcacctg atgaacaaga ggatatatct gtacagtctc
     4441 caatcgaact tctaagacag tccccgttct ttgaaatatt ttctgaacaa gatctatata
     4501 agttatcaga gatagctgaa aagaaatatt attatggtgg cgacagagtt tttacacagg
     4561 gtgaacaagc ctcgggtatc gatctgctcg tctcgggtag aaccgccatg tgtttttctc
     4621 ctgaagctac aaccaaacca ttagatgacg accaggtaga agattctgct tcgctaagac
     4681 ttgttcaaaa aaccggatat gttgtaggtt gggccggtgc gtttgacggc tttgtaaatg
     4741 actgcacagc ggttgctaca agaaatgccg ttatttatca tttcaaaaaa acttctctgg
     4801 ataaactttt taaagacaat tctgttcttg cattaaattt tgttaagaga ctactttggt
     4861 tattatcaaa ccagttgaga aatgccagag caaggcttat ttcccagcac tacgagcttg
     4921 aaattacatc tatacgcaac cttatggagc aaaacgcggc acagctgagt gttacttctg
     4981 cactgcataa ggttgctcac ctgctaaccc atacctacac tattggggat gcattcaatc
     5041 ttttattcca ggtagaaaaa gatggtagta cacttgaaaa aggtcttgca agattatgcc
     5101 tcgatatatt gggaaaagta tataaggaac atctcttttt cgaggggcta aaaaaggttt
     5161 atcagtttgt ctctgaagca cctgacaaca tgacaaccaa gggcgtaagg gtacagtcat
     5221 ccaggaaata tgtagagcta ttcaagcaaa ttccccatgt agtagagggt tgggaaaatc
     5281 ttcccgaaaa agcaggaaac atttttattt ctaaccattt agtaaatgat ccatataaca
     5341 cacttccaaa caactttcag ataacactcg attcacactt cataagctcc atgattttat
     5401 acaaaaaata tggtgatccg ggtgtaagag tcgtcagggt tccaagatca gaagaatttg
     5461 gccatcagaa ttattataac aaacttggcc atataaatgt ctatactaag gagtctgaca
     5521 gttcagaaga atcgcttaga atacaaaaag aacaaaataa gctattttac aaaactgcta
     5581 cagaatatat taaatccgga caaaacctga tgcttagccc cgaaggtaca agctacagga
     5641 cagaggactc acccggaccg tttaagcccg gtgctttcag acttgctgca tcaattgatc
     5701 cggaaccttt tattgtacca atagtcgtag ctaattttga caagagacta aatcgcaatg
     5761 ttttttcatt gatcatcaaa gagccgttta aattatcaga cttagttgat aaacctcagg
     5821 aaaatagaga tggtttagct caatttctgg atgaactgca aaataaatac aaaggcgatg
     5881 tacaagaagc tatagcactt gcaaataaaa cagcttcaac caagattaat ttaaaactct
     5941 ttgaaagagt gaaggaagac tctagagtat atagtaaaac caatatgact atagatgaat
     6001 taatgtttga aggcaatgta aaattactgg aaagaagaag agtaggtaca aaggaaaacc
     6061 cggttgtttt ttatggaagc tcctctcttc gactttggga gaccattgaa caggatttcc
     6121 ccgggcatga tatattgaat cttgcatttg gcggatcaac cattgattac tgctcatatt
     6181 atttcgatag gctaataaaa ccaagcaata ttaaatcatt cgtattctac gcaggagata
     6241 atgatatagg gcatggaaaa tctgctactc aggtgtttga ttcttttctt attttatata
     6301 acaagttcag agaatcattc accgacacta aatttacctt tgtttcaatc aaacccagta
     6361 ttgagagagc aaaatttata aacagaatac aaaaggcaaa caagcttata aaagagttct
     6421 tggaaagaga gccaaacaca ttttatctaa atgttcatga tcagatgcta aacggagaca
     6481 aacttgcaaa gcctgagctc tatactgaag acgagcttca tatgagtgaa aagggttatg
     6541 agctatggaa ggaagttttt ctagagaatg cggatgagat attttaaagg ttatctgaac
     6601 tacttgatct gggaactaac cagtaatata caacaccaga atatttatct aatgcaaaat
     6661 atgtatcgtg tcttcctatc acatagttac ccgtagatag agtctctgtt tttaaattat
     6721 aaatttctaa actagaattt ttcacatcat ctacatcaaa agcacatcta ttatataaaa
     6781 gtccaaaata aggtggttct tcatatgagt catttacttg cttataccct ttttggttaa
     6841 gtatcctcac aaaatcatct gaatgagatg ctggtatata aaatcttacg caagctgcac
     6901 tatacgaagc accgtttaca atttctattc tttttgaact ctctggtata tattcaggta
     6961 cccaacccct ttcaaataac ccctcgtatc ttgcatggtt ataatcgtaa tacttatgag
     7021 ctttgcctcc aaacgtcaaa aatagcaata atataattat tactacaata gtagttttaa
     7081 aaattatctt taattcatac tttaaagtac tcatatcaat aactttaata taaaatattc
     7141 actttcccac tcgtactatt ctgtaattcc gtttccaact ggtttagatt atcttcggga
     7201 acttctaatt tcatatctac attatcagtg taaattgaat cagcaatttc taagctgaat
     7261 ttatttagca cctgccgcac tgtattttca tactggtaat caaatttgag tgtaatggat
     7321 ttcaatgcta cacagtcttc aagcggaagt ttatcaagag cgtcttgtac agagcttgta
     7381 tatgcgcgga caagtcctcc tgtccccagc ttagtgccac cgaagtacct gctaactaca
     7441 actactatct ctccaatctt tttgtgctgc agcacactta gtatgggttt gcctgctgta
     7501 cccgatactt ctccatcatc tccaaatcca atatccgtgg tactatctgg atttcctgcc
     7561 acaaaagcat agcagttatg ggaagcgtct gaatacttct tgctcacagt atcaataaat
     7621 tcttttgctt tttgtttatc tgttactctt gtaacagaag ttataaactt actattttta
     7681 ataacctgtt ctacggtaac agttttggat ggaactttgt aagaagaatc ttctgccata
     7741 ataaaaaatg ttaattaacc ctcaccagac ctggcgcagt cgatgcaaag taaggctgag
     7801 gggtcaaact caagccgctt tggatcaatc tcaaggccgc agtgagtgca gtatccaaaa
     7861 tcgtcttcat ctattctttt aagggcagca tcaattcgtt ggattctaat ttcccttctt
     7921 ctctgtgact caaccgctat tgcctgagac tgcatcgcat ccattctgga taagcggcca
     7981 accttggatt gatcgagctc aacggttttt gtagcttctt gccctgcttc accttctttc
     8041 aatagctcgg attttatatg agctaacttg tccctgtaat attccagatc gatctcgtcg
     8101 ctgcccatgt ttgtaaccta attccccttc tttcttcgtt ctgtcacctg ctctccggct
     8161 atgccccagt tatcggtgtc cacttcatct attacaacga ccgtggtggc tgggttttta
     8221 ccaagtactt cttttagtaa attggtcgca ccctctataa gctttgcttt ctgttccttt
     8281 gttgcacctt ctcttgtgat cttgatattt acatacggca ttattactcc tttggaacgg
     8341 gtaatttttc actaatatac ataattgata tcatacagct attttcttcc cttaagctgc
     8401 tttaaggttt catactgtaa agagttcagt gcttcaaaaa gtacaccgtc cacttcgacg
     8461 ctatattgat tggatacatc aaccccagta agtggtacaa actttttgtc tccataaagc
     8521 cgtacaacat agagcataca atttagacgc gcctcgatct tggaatttga atttactgtt
     8581 atccatggtg atttatttga tgaagtatgc tgaaacatct gatttttata gttggtgtac
     8641 ttctcccact tttttcttgc ataaagatca ttttctgaaa atttccaaaa tttgagggga
     8701 tctgccaacc gttcctgaaa ccggataagc tgttgttcac tatcgactga taaataaaat
     8761 ttaaccagtt taagaccatc ttttatgagt ttatgctccc aatccaaaac cttacccata
     8821 aaatattcat actgcttttg tgagcagtaa cccatagtag gctctataag tgcgcggtta
     8881 taccacgagc ggtcaaaaaa tacaatttcc ccttttttag gaagctgttt ttcatatcta
     8941 tgaaaccacc tcttcgattc tttgggagtt ggaatgccca gttctactat cctgtagtat
     9001 ttgggcataa gtttttcagt aaatcggatt atggtagagc cttttccggc ggcatccctg
     9061 ccgtcaaagg taatacacat tctcttacct ttatttacta tatcctgctg tatatttaaa
     9121 agctctaact gtaggcggcg cttttcaata tggtaatctt ttagatttat acctttatac
     9181 ggattttcaa gatcaattat ttttttcatg ataaatcaaa cgatagagtc tgtgattaaa
     9241 gttattataa caaggaattg attaagatat tatgaaaata tcatccataa aatcactttt
     9301 cattataata tgacttatcc tgtaaacaaa attaataaac aaaccgaata tgaaagagta
     9361 tgatctcata atagtcggcg gcagtttttc aggtctttca tgcgccaaat cggcatctgc
     9421 aagaggactc caaaccgtac tattcgataa gaaaagagca ccggatgcat caacacagag
     9481 caccggaatt tttgtaaagg aaattgcgga tcaaattgac ttaccttctg tcctaacaag
     9541 aaaaatacat ggagtcaggc tttatagccc taacctgaat tctatagatt tggtttcccc
     9601 cggatattac tttttggcta caaacacaac tcaggttcta aaatggcttg cagaccaggc
     9661 tgcagacacg ggagcggaac tgctttatgg ccaaaacatc acagatgcat acataaaggg
     9721 caataaaata gtattaccta atcagcaggt aactacttca tatcttgtcg gtgctgacgg
     9781 cgtaagatca agagtggcta aaattttcgg cctcggacaa aacacacaag ttctgctcgg
     9841 aatagagtat gaagtagaag gtttaactaa tttagacgga gattttctcc atgtatttct
     9901 ggattctgag cttgccccgg gatatatagc atggatagtc ccgggagttc acttttcaca
     9961 agttggtctt gcatcttatt atcccaaaaa accggatata aaacagtttt atcaaatagt
    10021 tgaaaacaaa ttcggcggta aaaacactaa gatagtaagc agacgcggag gatttatacc
    10081 atgcgggggt actgtaagcc cactttcaaa aaataatgtc tgcctgcttg gagatgctgc
    10141 agggatggta tcaccaatga cagcaggcgg gattcatcct gcaattgaag taggaagact
    10201 tctgggaata gccataagcg actatttgca ggacaaaggc tttgctcctg aaaaagagat
    10261 agcaaaacat atcccatctt atcttttcaa aaatggtctt agaagattct tcaattttct
    10321 cactccacca aacttcctgc ttaatatttt gatagacaat ccccttttca gaaaatcggc
    10381 ccaaatcata ttcttccatc atcgtggatt attatccaaa aaagcgtggg ctgaaatact
    10441 tggcaaagag aaaaagtagt taataagtaa aaataatgat accctgccgg atttacagca
    10501 gggtatcatc acaatcttta agaattttac taaaaattat ccgtgttcca gatcaacttt
    10561 aaatgtgtct gcaaatcttg taagcacatg aaaagtgccc gctaccattg tgagttctgc
    10621 aatttcgccg tcattgaaat gatttctcat ttgagcaagt gattcttcgc tggctgcgac
    10681 attctgtgca agctccgttg tgtattgaac aatagccttt tctttatcat cgagtgcagc
    10741 acttgtagca tcagctgaag ctgcaatttt ttcatctgta agtccggctc tctttcccgc
    10801 tgctgtatgg tgagcggtac aataggaaca accatttaat ttactcgtag ttaatatggc
    10861 aagctcctta agatcgttgc taacctgtgt ctggccgagt gctgcaccta aaaaaggcat
    10921 gaaacccgag agcatttcgg gcgcatttgc cattgccttt attacgtttg gaactacacc
    10981 aaacttttgc tgaagcccgt cataaacggg ttgaaggttt tcagctgcct gttctttttc
    11041 tacatatggt attttagcca ttattggcct ccttgttttt catttgttga ataaacgttc
    11101 aacaatatac aaattttttt attttagtgt agataacata acattcacta tatctttcat
    11161 ctgctctccg cttgcgtccg actttcctgt aaccagaagc ccgtgcgtgc agcttgcaaa
    11221 gaatgatgaa agcgctttaa cattggattc tttagaaatt tcgcccagct gctgagcctt
    11281 tagcagacat tcataaaaag ctgattctat attttttagt atcccgttta ctatatctgc
    11341 ggtctgatca tcatgtggtg cgagttctac taccgaattt acaacaaaac acccgataca
    11401 cctcttatca tatgggcgct ggatcatttc gttaaaaaac ttttttatat tttctgtggg
    11461 agactctcct tcattcaaaa tcttaatagt atttgccgta acgacttcgc cgtagtgctt
    11521 aatagactca agaaacaggc tatgcttatc tccaaaggtg ttatagatgc ttccgggctg
    11581 tatgcccatc tcgtctacaa ggtctttcat acttgtagct ttatagccct tttgccaaaa
    11641 gactctcatg gcctttgata aggtctcgtc tttgttgaat tcgtaatgtc ttcccatgct
    11701 ggttatatta taacaaattt tgaacgcttg ttcaagaata actttattct agaatttata
    11761 ttttttattc agggattact tattgaaatt attgatttaa tcagtcttgt tataaaactt
    11821 aagaaaagaa atcagggaaa gaactaaaat ctttccctga ttagtgatca attatttact
    11881 tcttatcttt atatgggcag tcttccttct tgtctttata gctgcattct ttgtcaccgc
    11941 actctttatg cccgcaatct ttaccgtccc atttttgctc ccatttttct ttcatgatgc
    12001 cggaaagggt tacaatctgc tcatctgaaa gaagcgctct cattttaagc ttcatctcaa
    12061 gcttaagctg catcttctct gtcttcttgg ctattttttc attatgtttt gccgttatag
    12121 cttcgtctgt agcctttgga tcacccatga gatcgtaaag ctcttttttg attgtcttta
    12181 tttcggccca taattctgcc tttttacccg cataactttc atgtattgca ttcaaatcag
    12241 caatctgttt ttccgaaagt cctacttttg ttgcaatctc ctcattttcc caccacttgt
    12301 agtgcttacc tttataatgg tgtgctgcaa atgccgatac agatagtgct aaaacaagaa
    12361 gaaaaaccga taatgataat cttttcatct aaaaacctcc gtaaatatgt gctgttttct
    12421 tataaactac taattctaac cctttgtttc aataaataaa ttcattttag agttaaaata
    12481 atatagaata ttattactca aatttttact tatactacta aaatggaaaa taaacaaaaa
    12541 ttcatagccg cagccgtgca ggcatcaccc gtatttctta ataagacaaa aactattgcc
    12601 aaggtaaata aattaatagc cgaggctacc aaaaacggtg cagaactagt cgtattcccc
    12661 gaagctttca tacccaccta tcccttctgg ccaaaagaca tcggcacgcc ggagggaagg
    12721 aaacttatta ttgatgcata caccgatctt cataaaaact ccgttgaagt cccaagcgca
    12781 gatactgaga agatcggcaa agctgcaaag aaagccgggg tatatgtagt aatcggcgta
    12841 aatgaaaaag agggcgggac tttatacaat acgattttat actttggaaa agacggctct
    12901 ttgctcggaa agcacaggaa gtttatgtcc atagattctg aaaagtgcat ctgggcaaac
    12961 ggtacggcaa aagacgtgac cgtggtaaat acagacatcg gcaaagtagg aggacttttc
    13021 tgttatgagc accatatgac ccttgcaaag tatgcaatgt ttcaaaaggg cgagcagatt
    13081 catgtgggcc tatggggagg gcacgggttt gtaaaaaaca caatggactt tgcaagcaaa
    13141 cagtatgcat tcgagggaca gacatttgtt atcatttcat ccatgacaat tacagaagac
    13201 caaatccccg attcctttcc cctaaaaaaa cagaccatgt gggactaccc gggcggaagt
    13261 ggaattatta atccgcgtgg ggaatacatc gcaggcccag tgtatggtaa agaagaaatc
    13321 gtctacggcg aaattgatac cgaccttatc ctgagagcca aggccgtaat agacggcacc
    13381 ggccacttct caagaccaga tatttttcag ttggaagtta aggagtgata agtttattct
    13441 tttttttaac ctaacccgaa gtgccccctc ttatttactt ttcgaacaaa aggtagtaaa
    13501 ctattaacca aaataaatta ctaaagatta ctatgcatat tgttaaatta ttactatttc
    13561 tgaatataat tattgggagt aacttagttt ttgcacagga cataccacaa gcagaattcg
    13621 ctaaatgttc attaactaaa aatgatatac aaagattaag ttgttttgat aacctttcaa
    13681 aaaaatatga attaaataaa cctgcatcca aaatagttaa caagggaaat tgggctgtac
    13741 aaactgatat ttcccctatt gatgattcta ctaatgttta ccttgctgta cctggcgatc
    13801 aagcatttaa aggttggtta aagagttata atcctcaact gatgttaaga tgtaaggaaa
    13861 atgtaactga attgttaatt catacaggta tggctccaga tgttgaatat ggccatagag
    13921 gtgctacaac tactttgaga tatgacaaag aaaaagcata taagctaaga atgtctaaat
    13981 caacagatgg cgaagcatta tttttcccaa atcctataag caatataaaa aaaatgatga
    14041 atcatagtac tctattattt caatttacac catacaatgc tagtcctact atgacaacat
    14101 ttaaaatttc tggtttgagt gaagcagcaa aaccactaag agaacattgc ggatggtagt
    14161 gctcataact aaaattaaat atattattta atatgttaaa gtcatgatac atagcttttt
    14221 catatagata tcaattaatt aattaactaa tattttagct cttaaatata actattatgt
    14281 acatgaagtt aaaaatagtt tcattaatgt ttttatacct ttcattatat tccatatatt
    14341 taccaacata tgcagctgaa actactactt acggcaattg gacagtcaca aaaaaagtag
    14401 atgaatttac cggcgataaa tcactagagg ctaagataag gagtataaat ataataactg
    14461 gttggctttc tcaaaagggt aaagcttata tttctattca ttgtggcaaa ttaaactatt
    14521 taacatttat ctcaatagga atagaaggtg gtgtcggatt taataattca tttatggact
    14581 ttaaagttga tgataatgat ggagtacaaa cattttcatg gaatggagat ataggagatg
    14641 atataatttt ttctgcggta ggcaaaggag caattttctc tgaatcttct aaaacaccga
    14701 taatgtttga gcaaatgaag acaggtaaaa aaattaaaat acgtatcaat cccttcaaaa
    14761 cagaaagaca agttgtcgaa ttcaatttaa atggttctga taaagcacta acagaagtag
    14821 aaacattttg cacagaattg aataaatagt taacaaaaga tatagcgtca taactactat
    14881 tatttgctta aatctgcacc gcaaaatcta cacttaattg catccatctt tacattctca
    14941 gcgcattctg gacatttttt ggtagcatcg ttttcttcag ctttcatctc tttgcccata
    15001 tgacgagcta taaaaaatcc aattaaacaa caaacagcca taataataga taagcctcct
    15061 gttaacattg caaatattcc accaccaata gctgcaccag ctgcctcagc actactctgt
    15121 gtttccaatg acgaagcggc ctttccccca acaataaaac ctattggtat aaatgcaaac
    15181 cattcagctg caccaatcaa aaaccctctt ttaaatacct tagaaaggct tttagctcct
    15241 ttaagtataa agtaagatgt tataatccat cctcctaaga aagttatagc aataaatagt
    15301 ccagacggtc ctgtagaaga accaacatca gaaaatagca tggcagaacc catgtaaatc
    15361 aaaaaccctg aaaacaatcc cattatgata gctacaacat atttcattct ctgtccctcc
    15421 tataataagg atagctatat tatataagat tctgactagc tagttcaagc actttttaaa
    15481 ttccaattga atttgtgata gttgatgtgt tatcatttcc attaaaaaca ctcagatgat
    15541 taaaaaacga aagatctcta ttttgctagc cttcatttgc ctattactag ggtcagtaat
    15601 atatatacta tttcgcccca tgagtttaat gttgttcaac tggattgaat tattacgctt
    15661 tacagattca ctcatcatta tgcgtacttg gattactggt atacatatcc tattaccagc
    15721 atggattgtc tattcactac cttttgccct ttgggtgtgt agttacttat ttttcattga
    15781 gtatatatgg gataagccga tgtctatatc acgcaacata tggttttggt gcttacctat
    15841 tatagctctg gctgtcgaaa tttcacaaag tatatcaata ttgccaggaa tatacgattc
    15901 tactgattta ttattacttt tagttgtgat aattattgga attgtattaa cattctacaa
    15961 tagaatctat gat
//
LOCUS       DBNGSV010000383         3508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1660908, whole genome shotgun sequence.
ACCESSION   DBNGSV010000383 DBNGSV010000000
VERSION     DBNGSV010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1660908"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1501
                     /locus_tag="AC159U_13715"
     CDS             68..1501
                     /locus_tag="AC159U_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR3338152.1"
                     /translation="MNFSNYKTEDFYDEYFEKPGSPRNGAADLIKRIDSLSDGELLKR
                     QKGAEAALFNMGVTFTVYKKEAGIERIFPFDIIPRIIEHSEWAQIETGLKQRIEALNL
                     FIDDIYHDKEIIKDKIIPEELITSSKSYKKQCEGINPPKGIWCHITGTDIVRDKDGSF
                     YVLEDNLRCPSGVSYVLENRLVLKRVLPKLFADINIRPVDDYHYRLLDTLQNLVPDSI
                     SNPSVVVLTPGIYNSAYYEHSFLAQQMGVELVQGTDLVVSDGYVCMKTTKGLEKVDVI
                     YRRIDDDFIDPNAFNPDSLLGVPGLFEVYKNGRVSLANAPGTGIADDKTIYAYVPKII
                     KYYLDQDPILSNVPTYVCWDEKEKQHVMDNIEKLVVKPANESGGYGIVVGPHSTKKQL
                     EKAKEQINSDPRNFIAQPTLSLSRVPVIVDDHFEGRHVDLRPYILYGKDIYVMPGGLT
                     RVALKKGSLIVNSSQGGGSKDTWVLGK"
     gene            1505..2452
                     /locus_tag="AC159U_13720"
     CDS             1505..2452
                     /locus_tag="AC159U_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR3338153.1"
                     /translation="MLSRVANSLYWMCRYIERAENIARFVDVNLNLLLDLPYQETEQW
                     DPLVIITGDNEIFKQKYGEPNRDNVIQFLTFDTTYDNSIISNLQKARENARSVREIIS
                     TEMWEQINKYYFFVNDASSQSEIPENLYEFYNKTKLESHLFTGIMDTTMSHGEGWHFG
                     RIGRLLERADKTSRILDVKYFILLPSVDMIGGPIDYIQWAALLKSASALEMYRKRYRR
                     ILPDNVADFLIMDDDFPRSLRYCLINSQNSLRDITGSSHGTYQNSAERYLGKLSSELD
                     YSQIDEIIKMGLHEFLDKFQYDLNIVGNSIHKTFFEVEI"
     gene            2449..3192
                     /locus_tag="AC159U_13725"
     CDS             2449..3192
                     /locus_tag="AC159U_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HIR3338154.1"
                     /translation="MTFCVGMKVEDGLVGIADTRITSGTQYITARKLTVHTHGRHSLF
                     IMTSGLRSVRDKALTYFSEILEDENESYDKLYKAVNAFAKQVRRAYEEDKDALEESNL
                     NFNLYSIIGGQFEKDKEHKLYMLYPQANWVEVGKGNPYYIIGESSFGTPILDRALKYN
                     SPMEKALKAGYLAFDATKTSANNVDFPLDIVLYKKDSYKFIEHRYEREDLDSVSKWWQ
                     ESLIKLVKNMPNEWTDIPISILNEMSEGE"
     gene            3189..>3508
                     /locus_tag="AC159U_13730"
     CDS             3189..>3508
                     /locus_tag="AC159U_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HIR3338155.1"
                     /translation="MKSYRVEHTTTYTYSREVFFEPFTLRLKPKCNSFQKLHEYGLSI
                     IPEPQGINETLDLEDNNIITAWFEGTHKKLVIISNCIVEPLNINPFSFLITDDSFYTL
                     PVNNS"
BASE COUNT         1267 a          588 c          677 g          976 t
ORIGIN      
        1 atgtcattgt gaatttgtat aagttggtta caatgagcat tataatttct atttttatta
       61 aagatatatg aatttttcaa actataaaac agaagatttt tacgacgaat attttgagaa
      121 accaggcagt ccccgaaatg gagctgccga tcttattaaa aggattgatt cactatcaga
      181 tggggagcta ctaaaaagac aaaaaggagc cgaagcagct ctttttaaca tgggagttac
      241 ttttacagta tacaaaaaag aagccgggat agagagaata ttccccttcg atatcattcc
      301 cagaataatt gaacacagtg aatgggcaca aattgaaaca ggtctaaaac agcgaataga
      361 agccttaaat ttatttatag atgatattta tcatgacaaa gaaataatta aagataaaat
      421 cattcccgaa gaattaataa catcgagcaa atcgtataag aaacaatgcg aaggaataaa
      481 cccaccaaaa ggcatatggt gtcatataac aggcaccgat attgtaagag ataaagacgg
      541 tagtttttat gtattggaag ataacttgcg ctgtccatcc ggcgtgtctt atgtactgga
      601 aaaccggctt gtattaaaac gagtactgcc aaaacttttc gctgatataa atattagacc
      661 cgtcgatgac taccattaca ggctactgga tactttgcaa aatcttgtac ccgattctat
      721 ctctaaccca agcgtagtgg ttcttacacc gggaatatac aactcagcat attatgaaca
      781 ttcgttttta gcacagcaaa tgggtgtaga actggttcag ggtacagacc ttgtagtatc
      841 tgatggatat gtatgtatga aaactacaaa agggcttgaa aaagtcgatg taatttatag
      901 acgaattgat gatgacttta tagacccaaa tgcctttaat cccgattcct tgttaggagt
      961 cccgggtcta ttcgaagtat ataaaaacgg tcgtgtatct cttgcaaacg caccgggaac
     1021 agggatagcg gatgataaaa ccatatatgc ttatgttccc aagattatca aatattattt
     1081 agatcaagac cctatcttgt ccaatgtacc cacatatgta tgctgggatg aaaaagagaa
     1141 acaacacgta atggacaaca tagaaaagct tgtagtaaaa ccagcaaatg aatccggagg
     1201 gtacggaata gtagttggtc cgcattctac gaaaaaacag ctggagaaag caaaagagca
     1261 aattaactct gacccgcgca atttcatagc acaacccaca ttatcacttt caagagtgcc
     1321 tgttattgta gatgatcatt ttgagggaag acacgtagat ttaagaccat atatactata
     1381 tggaaaagat atttatgtta tgcccggagg acttacgcgt gttgcactta aaaaaggttc
     1441 actaatagta aattcttcac aaggtggagg aagtaaagac acctgggtgc tcggtaaata
     1501 atatatgctt agtagagttg caaattcact gtactggatg tgcagatata tagaaagggc
     1561 agagaacatt gcaagatttg tagatgtaaa ccttaacctg ctgcttgacc tgccttatca
     1621 ggaaactgaa cagtgggatc ctcttgtaat tattacaggc gataatgaaa tatttaaaca
     1681 aaaatatggt gaaccaaata gagataatgt tatccagttt ttaacttttg ataccacgta
     1741 cgacaattca atcatatcca atctccaaaa agcaagagaa aatgcaagat ctgtaagaga
     1801 gataatctct acagagatgt gggagcaaat taataaatat tacttttttg ttaatgacgc
     1861 ctccagtcaa tctgaaatac ctgaaaacct gtatgaattt tataataaaa ctaaacttga
     1921 aagtcatctt tttactggaa ttatggacac aacaatgtct catggtgaag gctggcattt
     1981 tggcaggata ggcaggctac tggaacgtgc tgataaaacc tcgagaattt tggatgttaa
     2041 atattttatt ctgcttccaa gcgtggacat gattggagga cctatagatt atattcagtg
     2101 ggcagcacta ctgaaatcag caagtgcact tgagatgtat agaaaaagat acagacgaat
     2161 ccttcctgat aatgtagcag actttcttat tatggatgat gattttccaa gatcactaag
     2221 atattgcctt ataaattccc aaaattcact ccgtgacatt accggcagct cacatggaac
     2281 ttatcaaaac agcgccgaga gatacctggg caaactctca tcagaactcg actattcaca
     2341 gatagatgaa attatcaaga tgggacttca tgagttttta gataagtttc agtatgattt
     2401 aaacatagtc ggaaactcca ttcacaaaac ttttttcgag gttgaaatat gacattttgc
     2461 gttggaatga aagtagaaga cgggcttgtg ggaattgccg atactcggat aacttcaggt
     2521 acacaatata tcactgcaag aaaactgacg gttcacacac atggaagaca ttccctattc
     2581 ataatgacat caggactcag gtcagtaagg gataaagctc taacttactt cagtgaaata
     2641 ttagaggatg aaaatgaaag ttatgataaa ctatacaaag cagtaaatgc atttgcaaag
     2701 caggtaagaa gagcatacga agaagataaa gatgcgcttg aagaaagtaa tttaaatttt
     2761 aatttgtatt caataatcgg cggtcagttt gaaaaagata aagagcacaa gctgtatatg
     2821 ctatatccac aggcaaactg ggtagaggtt ggaaaaggta atccctacta tattataggt
     2881 gaatcaagct ttggaacacc aatactggac agggctctca aatataactc acctatggaa
     2941 aaagcgctta aagccggata ccttgctttt gatgctacca aaactagtgc taataatgtg
     3001 gattttccct tagatatagt tctgtataaa aaagacagct ataaattcat agaacacagg
     3061 tacgaaagag aagatctgga tagcgtttcc aaatggtggc aggaaagtct gattaaactt
     3121 gtaaaaaata tgccaaatga atggacagat attcccatta gtatacttaa tgaaatgtca
     3181 gagggtgaat gaaatcatat agagtagaac atacaactac atacacatac tctagagaag
     3241 tattttttga gccatttacg ctaaggttaa aaccaaaatg taatagtttt caaaaactac
     3301 atgaatatgg gttatcaatc atcccagagc ctcagggaat aaatgaaaca ctcgaccttg
     3361 aagacaataa tataataaca gcgtggtttg aaggcaccca taaaaaatta gttatcataa
     3421 gtaattgcat tgtagaacct ctaaatatta atccatttag ttttttaatt acagacgact
     3481 ctttttatac attacccgtt aacaattc
//
LOCUS       DBNGSV010000384         6028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1592414, whole genome shotgun sequence.
ACCESSION   DBNGSV010000384 DBNGSV010000000
VERSION     DBNGSV010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1592414"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1240)
                     /locus_tag="AC159U_13735"
     CDS             complement(80..1240)
                     /locus_tag="AC159U_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338156.1"
                     /translation="MHRTKISVVVVSIFCLSLIFTACGGTSDSELNNSVVSGKVEGIL
                     ISKEVKKQNRTILSSILDKLSFLKELNAQSAGVLVNVFKDGVLVQSTVTNSDGNFIVS
                     TSGLVTFEFRFDGQSIFTDVIIPENNVITYNVTLDFNNDLVLLNQLDIGIQCEGGVTQ
                     INRDIHINGQGGDCIKAENDCNLIINSNMITLEQCSNCIKTEDESSVSINTNEVLCDS
                     EEDGIDANDDSDVDINGDIVEIISDENGVDADDNSSVDIEGTDCIIEGAQGDISTDEG
                     AEVNTDCEVLNDDPSDSPSDSPSDSPSDSPSDSPSDSPSDGPSDSPSDSPSDSPSDSP
                     SDSPSDGPSDSPSDSPSDSPSDSPSDSPSDGPSDSPSDGPSDNPSDGPTPPP"
     gene            complement(1307..2011)
                     /locus_tag="AC159U_13740"
     CDS             complement(1307..2011)
                     /locus_tag="AC159U_13740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3338157.1"
                     /translation="MSLNINSISVVLPAFNEEENIGYTIQNCVDYMQVNNYDYEVIVV
                     NDGSSDNTKDIVQKAKTDNSNVVLVDHDINKGYGAALRSGFESAQKDYVFLMDSDRQF
                     NIEDIEKLFLYLDEQTVVVGYRQNRADPVIRKINAYLFHIYIKLMFGLNLRDIDCAFK
                     VFPTQAYQQIKPIISDGALFSTEFIIRLLNKGYGIKEIAVNHYPRLKGSQSGANISVI
                     LKMFAESWNLRKILNS"
     gene            complement(2031..2759)
                     /gene="rlmB"
                     /locus_tag="AC159U_13745"
     CDS             complement(2031..2759)
                     /gene="rlmB"
                     /locus_tag="AC159U_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3338158.1"
                     /translation="MIIYGKNSVKEFLFNTPSEITEVFVFKGSKQSFYSDIEKLCKKS
                     RLKLTALNESEISKLCKTTKHQGIAANIKEFEYTDLDDYLRGSDGSNSSNKLVVVLDH
                     IEDPHNLGAIIRSVNVLGGDAVIISKDRSCGITPAVVKASSGAVKYVPVIQEVNLSRV
                     LDKLKKSDFWVIGADQGADKNVYEQDFSDRNIALVMGSEGRGLSTNIKKQSDYLVKIP
                     QAGQITSLNVSVAAGILIYELTKK"
     gene            complement(2756..4105)
                     /locus_tag="AC159U_13750"
     CDS             complement(2756..4105)
                     /locus_tag="AC159U_13750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3338159.1"
                     /translation="MVLIKRVFVFLIIAAVVTGVIYFLNYIEWYKPQVQVGLDSGFIG
                     TKPIKVNITDKGRGLKSTVAKLQFETGSVTLVQKNYTDAPKSDSFDLTLDKQDLTEGP
                     AKLMIEATDGSYLNFLHGNKTVFEKEVVVDTQPPSIETISSQDYINHGGSGFVVYKVS
                     SDTKSSGVLVGDIYFPGYSGHFADQNVYVCFFAFPYNIDKDSDVVITAYDKAGNVAKN
                     PFYYNVKPVTYRKSNINISEKFILNKMQSLIKGPKPSDLKQIFLKVNSELRKENDSTI
                     ANVSKETEPKILWNGNFHQLSRSQVEANFADERTYVLNEEPIDKQYHLGYDLAVTKRY
                     PIEAANAGVISYAGSLGIYGKTVIIDHGMGITSLYGHMSSLNVKVGDVVKRKQIIGKT
                     GTTGLAAGDHLHYGVYIQGHAVRPVEWWDPKWIKDNVVTKLLDAEQEFGLKKPEAEN"
     gene            4213..4794
                     /locus_tag="AC159U_13755"
     CDS             4213..4794
                     /locus_tag="AC159U_13755"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3338160.1"
                     /translation="MDFQKFNPDIHNCVEVAKLVAKSTDYHNLTYSSSSNLMDSLSKN
                     IADNQLNFENIHILTKQSMYLGIIIFSYKKKSNSLFNDLKFLLTLKLNQFFQRLYIFL
                     IENEIKMTDHDVYLDLIYIEPDFRNKGIGTKSINYLIELVKLQSKKQILLHVDRANLS
                     ARSFYEKLGFKYMMNNSGESKNNYLAMSYNIKA"
     gene            4794..5723
                     /locus_tag="AC159U_13760"
     CDS             4794..5723
                     /locus_tag="AC159U_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3338161.1"
                     /translation="MRKEFKNEFSWSFSRDNTFNECRRKYFYNYYGSWGGWKRDSIDE
                     VTRSLYVLKNLVNMKIWKGDAVHKEISRVLKSFETTDSIGSFENSVKRTTRIMRDEFR
                     FSKAKEYWTTDKSLRKVNALFEHEYELDVSDEEWIDNYNDVIKCIKNFYSSEIVEELK
                     SIGCENLITVDSITPTSFDYNGEIVYVNLDLAYKVGDEVKIVDWKTGTGESNPLQFVV
                     YVFHTNETLGTPLDKLSVIEYNLQENKQYVHKFSEQEITEAKDYIDNSMKSMKSCLYD
                     PEENMADISEFPRTDEDWRCNNCNFKKICFDLP"
     gene            5745..>6028
                     /locus_tag="AC159U_13765"
     CDS             5745..>6028
                     /locus_tag="AC159U_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3338162.1"
                     /translation="MFKDFKEQQIKTTEVTINIKHAGSGKPLLLLHGYPQTHVMWHKV
                     APKLTKDFHVICADLRGYGDSSKPENSEDNYTYSKRAMAGDMIEVMKALG"
BASE COUNT         1918 a         1136 c         1002 g         1972 t
ORIGIN      
        1 aaaggggaga taattattat caattatctc ccctgtgttt aattaattgt tagttatata
       61 ttgttgttat gcttattacc taaggaggag gagtcggacc atcagagggg ttgtcactcg
      121 ggccatccga aggactgtcg ctggggccat ccgaaggact gtcgctgggg ctatctgatg
      181 ggctgtcgct ggggctatct gatggactgt cacttgggcc gtccgaagga ctgtcgcttg
      241 ggctatctga aggactgtca ctcgggctat ctgatgggct gtcactcggg ccatccgaag
      301 gactgtcgct ggggctatct gatggactgt cgctggggct atctgatggg ctgtcgctag
      361 ggctgtctga agggtcatca tttagcactt cacagtctgt atttacttcg gcaccctcat
      421 cggttgatat atcaccctga gctccctcta taatacaatc agtcccctct atgtctactg
      481 aagagttatc atctgcgtct acaccgtttt catcactgat aatttctact atatctccat
      541 ttatatccac atcggagtca tcatttgcat caatcccatc ctcttctgag tcgcataata
      601 cctcattagt attaatagat actgaagatt catcttcagt cttaatacaa tttgagcatt
      661 gctctagagt aatcatgtta gaattgatga ttaaattaca gtcattttca gcttttatac
      721 aatcgccacc ttgcccgttt atatgtatat cacgatttat ctgtgtaact ccaccttcac
      781 actgtatacc aatatctaac tgattgagaa gcaccaggtc attattaaaa tcaagcgtaa
      841 cattatatgt gattacatta ttttcgggaa taatcacatc tgtaaatatg ctctgtccgt
      901 cgaatctgaa ttcaaaagtt acaagccccg aagtagaaac tatgaaatta ccatcactgt
      961 ttgtaacagt tgattgtact aaaactccgt ctttgaaaac atttacaagt acaccagcag
     1021 attgagcatt taattccttt aaaaatgaga gtttatctaa tatagaggat aatattgttc
     1081 tattttgttt ctttacctct tttgaaatca atattccctc aacttttcca ctaacaacac
     1141 tgttattcag ttcagagtca cttgttcctc cacaggctgt aaatattaaa gataagcaaa
     1201 atatcgatac aacaactaca gatattttag ttctatgcat tactaaggtc accatcctac
     1261 tatagtatta aataaattat atgagatgct aataagaagt caactctcac gaatttagaa
     1321 tttttctcaa attccaggac tcagcaaaca tttttaaaat tacactaata tttgctcccg
     1381 actgactgcc ttttaatctt ggataatgat ttacggcaat ttctttaata ccataaccct
     1441 tattaagtag tcttataata aactctgtac taaaaagagc accatctgag attattggtt
     1501 ttatttgttg atatgcctga gtgggaaaca ccttgaatgc gcaatctata tctcttagat
     1561 taagaccgaa cataagctta atatatatat gaaataaata tgcattaatc tttctaatta
     1621 caggatctgc cctgttttgc ctgtatccta caacaactgt ttgttcatcc agataaagaa
     1681 agagtttttc tatatcctca atattaaact gtctgtcact gtccattaaa aaaacataat
     1741 ctttttgtgc ggattcaaac ccacttctaa gcgcagctcc ataaccctta tttatatcgt
     1801 gatccacgag cactacattg ctgttatcag tttttgcttt ttgaacaata tctttagtat
     1861 tgtcggatga accgtcattt acaactataa cttcataatc ataattatta acctgcatat
     1921 aatctacaca gttttgtatt gtgtagccga tattttcttc ttcattaaaa gcaggaagta
     1981 caacagaaat agaattaata tttaaagaca tagaatataa tgaagatgtt ttacttcttt
     2041 gttagttcat agatcaatat ccctgcggca accgacacat ttaaagaagt aatttgccct
     2101 gcctgaggaa tttttaccag ataatcgctc tgttttttta tattagtact cagacccctg
     2161 ccctctgaac ccatgaccaa tgctatgttt ctatctgaaa agtcttgttc gtaaacattt
     2221 ttatcggcac cctgatcagc tcctatcacc caaaaatcag acttctttaa tttatcaagc
     2281 acccttgaaa gattaacctc ctgaattaca ggtacatatt tcacagcccc cgatgatgcc
     2341 tttacaacag caggagtaat cccgcatgac ctgtcttttg aaataatcac agcatctcca
     2401 cccagcacat ttactgacct tattattgca ccaagattat gggggtcttc aatatgatct
     2461 aacactacta caagtttatt cgatgaatta ctaccatccg atcctctaag ataatcatcc
     2521 aaatcggtgt actcaaattc cttaatattt gctgctatac cctgatgctt tgtagtttta
     2581 cagagctttg atatttctga ttcatttaag gcagttaact ttaacctgct ctttttacat
     2641 agcttttcta tatcagaata aaaagattgt tttgagcctt taaatacgaa aacctctgta
     2701 atctctgatg gggtattaaa taaaaactct ttgactgaat tctttccgta aataatcaat
     2761 tttcagcttc tggcttcttt agtccaaatt cctgctcagc atcgagcagt ttagttacga
     2821 cattgtcctt aatccactta ggatcccacc attccacggg ccttacagca tggccctgaa
     2881 tatatactcc atagtgcaga tgatcacctg cggcaagacc agtagtgcct gtttttccta
     2941 tgatctgttt tcttttgaca acatccccta ccttcacatt caatgaactc atatgaccat
     3001 aaagacttgt aatccccatc ccatgatcga tgattacggt ctttccataa ataccaagac
     3061 tacccgcata acttataact ccggcatttg cagcttctat tgggtatctc tttgtaacag
     3121 caaggtcata accaagatga tactgtttat ctataggttc ttcgtttagt acatatgtcc
     3181 tttcatcagc aaaattggcc tccacctgag acctcgagag ctgatgaaaa ttcccattcc
     3241 atagtatctt tggttcagtt tctttagata catttgctat tgttgaatcg ttttccttgc
     3301 gaagttcact gttaaccttt aagaatattt gttttaagtc agatggtttt ggtcccttga
     3361 taagagactg cattttgttg agaataaatt tttcgctaat attaatgttg cttttcctgt
     3421 atgtcacagg ttttacattg tagtaaaaag ggttcttggc gacattaccg gctttatcgt
     3481 atgcagttat tacgacatca gagtctttat caatgttgta tggaaatgca aagaaacata
     3541 catatacatt ttgatcagca aaatgtccgc tataaccggg gaaatatata tcacctacca
     3601 atactcccga agatttcgta tcagaagata ctttgtaaac tacaaacccg cttccaccgt
     3661 ggttaatata gtcctgagag ctaatcgttt ctatcgatgg cggctgtgtg tccactacta
     3721 cttctttttc aaaaactgtt ttattgccat gcaaaaagtt tagatacgag ccatctgtag
     3781 cttcaatcat aagttttgca ggtccttctg taaggtcttg tttatcgagg gtaagatcaa
     3841 atgaatcact tttaggagca tcagtataat ttttttgtac taatgtgaca gaccctgttt
     3901 caaattgaag tttagcaaca gttgatttaa gccctctgcc tttatcggtt atattaactt
     3961 taattggttt agtgccaata aaccctgaat caagacctac ctgcacctgg ggtttatacc
     4021 attcaatgta atttaagaaa taaattactc ctgttacaac cgctgctatt attaagaata
     4081 caaaaactct ttttattaat accatagcct cccctttatt atttaaagct attattaatt
     4141 gataaattat tttatattaa taattctaat ttataatcaa aaaattatta attttaacta
     4201 aaaatcatta aggtggattt tcaaaaattc aatccagaca ttcataactg tgtagaagtt
     4261 gcaaaacttg tagctaaaag cactgattat cacaatttaa cttattcatc atccagcaat
     4321 cttatggata gcctaagtaa aaatatagca gataatcagt taaactttga aaatatacat
     4381 attctaacta aacaatccat gtatctggga attataatat tttcatacaa aaaaaaatca
     4441 aatagtttat ttaatgattt aaaatttctt ttaacactta agctcaatca gttttttcaa
     4501 agattgtata tattcttaat tgaaaatgaa attaagatga ccgaccatga tgtatacctc
     4561 gatctgatat acatagaacc cgatttcaga aacaaaggga taggaacaaa atctataaat
     4621 tacttaattg aactggttaa attacaaagc aaaaagcaga tacttcttca tgtagataga
     4681 gcaaatctct ctgcaagaag tttttatgaa aaacttggtt ttaaatatat gatgaataac
     4741 tcaggtgaat caaagaacaa ttatctggca atgagctata atattaaagc ataatgagaa
     4801 aagagtttaa aaatgaattc agctggtcat tttcaagaga taacaccttc aatgagtgca
     4861 ggagaaagta cttttataac tattatggct cctggggcgg atggaaaagg gattcgatag
     4921 acgaggttac cagatctcta tatgttctaa aaaacctcgt aaatatgaag atctggaaag
     4981 gagatgccgt acataaagaa atttcgaggg tactgaagag ttttgagact acagacagta
     5041 ttggaagttt tgaaaactcg gtaaagagaa caaccaggat tatgagagat gaattccggt
     5101 tctctaaagc aaaggaatat tggactaccg ataagagcct tagaaaagta aacgctcttt
     5161 ttgagcatga gtatgagctt gatgttagtg atgaagagtg gatagacaat tataacgatg
     5221 ttattaagtg cattaagaat ttttacagct cggagattgt tgaagaacta aaaagtatcg
     5281 gatgcgaaaa cctcattact gtagatagca ttacccctac ctcatttgat tacaacggtg
     5341 agattgttta tgtaaacctt gatcttgcct ataaagttgg agatgaagta aagattgttg
     5401 actggaaaac aggtaccggt gagagtaacc cacttcagtt tgtagtatac gtgtttcaca
     5461 caaatgaaac ccttggcact cctcttgata agctatccgt aatagaatat aacctacagg
     5521 aaaataaaca gtatgttcat aaattcagtg agcaggagat aactgaggca aaggattata
     5581 tagacaatag catgaagagt atgaaaagct gcctttatga tcctgaagaa aatatggctg
     5641 atattagtga atttcctaga acggatgaag actggagatg caataactgt aattttaaaa
     5701 aaatatgttt cgatcttcca taaaacgttt taaaattaat ctttatgttt aaagatttca
     5761 aagagcagca aataaaaact acagaagtta ctattaacat taaacatgct ggcagcggca
     5821 agccgcttct ccttttgcat ggatatcctc agactcatgt gatgtggcat aaagtagccc
     5881 ctaagcttac aaaagatttt catgtgatat gtgcagacct aagaggctat ggtgacagtt
     5941 ctaaaccaga aaattcggag gataactata catactccaa acgcgcgatg gctggtgata
     6001 tgatagaggt aatgaaagct cttggtta
//
LOCUS       DBNGSV010000385         1888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1661588, whole genome shotgun sequence.
ACCESSION   DBNGSV010000385 DBNGSV010000000
VERSION     DBNGSV010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1661588"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..738)
                     /locus_tag="AC159U_13770"
     CDS             complement(124..738)
                     /locus_tag="AC159U_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338163.1"
                     /translation="MGFWEDITGVTAANTAAAAQTAGTEASIEEQRRQFDVMQGLASP
                     YVEAGANALQGQQALSGALGPEAQQAAIASIQAGPGFQSALQQGETSLLQNAAATGGV
                     RGGNTQAALAQFSPQLLNQAISQQYGQLGGLAGMGQASAMGLGQSAMQMGQNVGAGMS
                     QIGNIGAQQAIGGYNRQTGFIGDLFGGLMSLGGLGVKAKGAGIF"
     gene            complement(928..>1888)
                     /locus_tag="AC159U_13775"
     CDS             complement(928..>1888)
                     /locus_tag="AC159U_13775"
                     /inference="COORDINATES: protein motif:HMM:NF022584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019073 - viral DNA genome packaging
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="packaged DNA stabilization protein"
                     /protein_id="HIR3338164.1"
                     /translation="IIVTELADPTLILATKYGSSEIDPDPVVSLLKLRNEIYAVNRYT
                     IEVFNNIGGTGFPFQRVNGAQIQRGAVGTHAAVVYEDTIAFLGSGRNEAPAIYLGVNG
                     QTAKISTREIDEILIGFTEDQLSEVVLETLNDKSQAFLWVRLPDRTLVYDMTASKVVG
                     EPVWYIMSSGVTEYTQYRARDVVFCYDKWQVGDTESNKIGTLDNTISTHYGDTTIWEF
                     GTTVVYNESRGALFKKLELVALTGRNQGFEDSVITTSYSVDGRTWSQERPINAGKPGD
                     RLKRLVWWRQGSMRNTRTQRFRGDSNAYLAVSRLEVDLEPLGV"
BASE COUNT          501 a          464 c          310 g          613 t
ORIGIN      
        1 cttcttgatt ctttctatta acctcttgat caatcagacc accaccaagt tgggctatac
       61 cttgaccaat attagttaat agtgtctgtg gctgttgtga aaattgatat attaaatcac
      121 tcattagaat atacctgcgc ctttagcttt tacacctaat ccaccaagac tcattaagcc
      181 tccgaagaga tccccaataa accctgtttg tctgttatat ccacctattg cttgttgtgc
      241 accaatattt cctatttggg acattcctgc accaacattc tgacccatct gcattgcaga
      301 ttgtccaagt cccattgctg aagcctgacc catacctgcc agtccaccta attgtccgta
      361 ctgttggcta attgcctgat ttagtagttg tggtgagaat tgagccaatg cagcctgagt
      421 atttccacct ctgacaccgc ctgttgcagc agcgttttgt aaaagtgaag tctctccctg
      481 ttgcaatgct gattgaaatc ccggacctgc ctgaattgaa gctattgctg cctgttgtgc
      541 ttccggtcca agtgcaccgg aaagagcctg ttgaccctgt aatgcattgg ctcctgcttc
      601 cacataagga gaggcgagtc cctgcattac atcaaactgt cttcgctgtt cttctatact
      661 tgcttctgtt cctgctgttt gagcagcagc agccgtattc gcagccgtaa cacctgttat
      721 atcttcccaa aaacccataa taacctctct ttattcagta tagtgtataa tttagtatac
      781 atcaaggatt ctctaatgca tacacacgga gagctaagtc atttacgata gcaattaaga
      841 gttccaattg ttttattgtt tcatgatctt cagaaacctt tgctaattga tttcttgtca
      901 atctgacttt atcatctgtt acagccatta aacccccaat ggttcaagat caacttctaa
      961 cctggaaact gccagataag cattagaatc tccacggaac ctttgtgttc tggtattccg
     1021 catacttccc tgtctccacc atacaagtct ttttaacctg tctccgggct tcccggcatt
     1081 tattggtctt tcctgactcc atgttctacc atcaactgaa taagaagtag taatcaccga
     1141 atcttcaaac ccctgattcc tgcctgttaa agcgactaat tcaagtttct taaacaacgc
     1201 tccacggctc tcgttatata caactgttgt tccaaactcc catatcgtag tatctccata
     1261 atgagtggaa atagtattgt caagagttcc aattttattg ctttcagtgt caccaacctg
     1321 ccatttatca tagcaaaata caacatctct tgctctatat tgggtatatt ctgttacacc
     1381 acttgacata atataccata caggctcacc aaccactttt gaagcagtca tatcatagac
     1441 caatgttcta tctggaagcc taacccataa aaaagcctgg cttttatcat taagtgtttc
     1501 aaggacaact tcagataatt ggtcttcagt aaaccctatt aaaatctcat caatttctcg
     1561 tgtactgatt tttgcagtct gcccatttac tccaagataa atagccgggg cttcatttct
     1621 tccgcttccc aggaatgcaa tcgtatcttc atagacaact gctgcatgag ttcctacagc
     1681 acctctttga atctgtgcac cgtttactct ctggaaagga aacccagtac cacctatatt
     1741 attaaacact tcaatggtgt acctgtttac tgcatagatt tcatttctta atttaagaag
     1801 gctgacaact ggatcggggt ctatttctga agacccatat ttagttgcaa gtattaaagt
     1861 tgggtctgct aactctgtta caattatg
//
LOCUS       DBNGSV010000386         1008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1661619, whole genome shotgun sequence.
ACCESSION   DBNGSV010000386 DBNGSV010000000
VERSION     DBNGSV010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1661619"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1008
                     /locus_tag="AC159U_13780"
     CDS             <1..>1008
                     /locus_tag="AC159U_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338165.1"
                     /translation="GLLLKNTYLRDLSYTDIPYNTASVLTGWLADYDNGDEYGFTLIK
                     WKEWNNPGQVDIDEFTPFLFIRTLLDKIFDSIGYTIQSDFFDTDMFKKLIFVIPPLQR
                     YPEEFSLDYINIKAENDVPVVGPVTSNPYILPTQTQTPAIGANPYDTGTGIYTVPFTG
                     YYEISVRYSLSNIIGNASFSIAVLINGISFIALPNPIIFGNIITSDSSYSGTTQVLYL
                     NAGDQITLAVFEGTTVSYNSYIEFSVIGEADATFGNLLAWRYMVKDWKAIDFIMGLQH
                     MFNLRFEPNIQEKIIRIEPADAYLYRERATLTTATETLNTGFYNNTLIDSTPKIDLNK
                     DA"
BASE COUNT          308 a          194 c          199 g          307 t
ORIGIN      
        1 ggcctcctgt taaaaaatac ctatctgaga gatttatcct atacagatat cccttataat
       61 actgcctcgg ttttaactgg atggctcgct gattatgata acggagatga gtatggattt
      121 actctgatta agtggaaaga gtggaataat ccaggacagg tggacatcga tgagttcact
      181 ccttttctgt ttatccggac gctgctcgat aagatattcg actcaatagg atacactata
      241 caatcagatt tttttgatac tgatatgttt aaaaaactga tatttgtcat ccctcctctg
      301 cagcgttatc ctgaggagtt tagccttgac tatatcaata taaaagcaga gaacgatgtc
      361 ccagtggtgg gccctgttac atcaaatccg tacattttac cgactcagac tcagactccg
      421 gccatcggtg caaatccata tgatacaggc actggcattt atacagtacc ctttacagga
      481 tactatgaga tatctgtccg atattctttg agtaatataa taggcaatgc gagtttttca
      541 atcgctgttt taataaatgg catatcgttt atagctttac ctaatccgat tatttttggg
      601 aacataataa catcagattc gagttattca ggtactactc aggttttata tcttaatgca
      661 ggagaccaga tcacgctcgc agtttttgag ggtactactg tcagctataa cagttatatc
      721 gagttttctg taatagggga agctgatgcc actttcggaa acttgctagc ttggaggtat
      781 atggttaaag actggaaagc aatcgatttt ataatgggat tgcagcatat gttcaaccta
      841 agatttgagc caaacataca ggagaaaatc atcaggatag agcctgctga tgcgtacctc
      901 tatcgggagc gtgcaacttt aacaaccgct acagaaacac taaatacagg attttataat
      961 aatacgctca ttgatagcac tccaaaaata gacctcaata aagatgct
//
LOCUS       DBNGSV010000387         4458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_1800705, whole genome shotgun sequence.
ACCESSION   DBNGSV010000387 DBNGSV010000000
VERSION     DBNGSV010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_1800705"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..2384
                     /locus_tag="AC159U_13785"
     CDS             51..2384
                     /locus_tag="AC159U_13785"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3338166.1"
                     /translation="MADNSSFVLRYTNWVIRWRWAVLILSLLLAILLGTGVKRLGLAT
                     DYRVFFSDDNPDLLAFDALENIYTKNDNVLILIKPENGDVFNKDTIKAIYEITEEAWQ
                     TPYSTRVDSITNFQHTWADGDNLIVEDLIEEKNFTEKDIARAKKVAFSEPFIYKRLLS
                     EDGKTAGINVRIQQPGKSPAEVPEIAAFARDLRDKYRALYPNQDIRLVGVSMMNNAFA
                     ETPIKDFSFVMPLMFGVFTFALIIFLRSFLASGATLILVVISALTALGIAGFFGTFLD
                     PTSSSTPNIILTLAVADSIHILVSFFEARRSGKNRYDAIIESIDINAKPVFLTSITTA
                     IGFLSLNFSDSPPFRLLGNLTALGVMIAWFYSMTFLPAFLSVLPIKAPKRSELTYSFI
                     DKLSDFVISRPKQILLLLGSFVIVLILLIPTMNINDRYHEYFSKKLDIRNSMEFSVKN
                     LSGVYITSFSLESGDTNGVSDPEYLSKVSAFTDWLRARPEIAHVNSFSDTMKRLNKNM
                     NGDDPAFYKLPENRELGAQYLLLYEMSLPYGLDVNDQINIDKSSLRLDVTYADANLNT
                     IVQNSKEANKWINENGTESMKENRGAGTTLMFGNITRRNLDAMLIGTFVGFGLIAIIL
                     MIALRSVKVGLISLIPNILPSAMAFGVWALLIGEVGFAISVVASISIGIIVDDTVHFL
                     TKYHRARTELGHDTPDAIRYAFHTVGTAILGTSFIIAAGFAMLGMSTFRVTSYMGLLT
                     SLAIVSALITDFLLLPALLLILDKDKKKDKGTPFATN"
     gene            2419..3210
                     /locus_tag="AC159U_13790"
     CDS             2419..3210
                     /locus_tag="AC159U_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR3338167.1"
                     /translation="MKFKILMLILSILIPLSSVYSQTSEEKGKQIAIDADKADQGWGD
                     NKSVSKMVLRNKQGQESVRKLRRLALEINEPGLGDKSITVFDEPRDIEGTALLSHTKI
                     LKPDDQWLYLPAVKRVKRISSKNKSGPFVGSEFAYEDLLSQEVDKYNYKWIKDEKCGE
                     LECFVVERYPLYENSGYTKQVVWWDKQEYRPQKIDFYDRKDSLLKTLTYTKYNQYLDK
                     YWRADLFVMKNHQNGKSTDIVFESWEFRLGQDHSQFTASRLKRAK"
     gene            3200..4423
                     /locus_tag="AC159U_13795"
     CDS             3200..4423
                     /locus_tag="AC159U_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338168.1"
                     /translation="MPNNFSYHFLTLFLLVSYFLSGKQIYAGQWDIRGETGIELRIFP
                     DDPEFNSQDDTYFSPSFMIEPEILYETEKGNDLFEFVPFFRFDLDDDNRTHFDIRELN
                     WFHQGSNWSLLVGADIVYWGVTESRHLVNIINQNDQVEDIDEEDKLGQPMINFTYDSD
                     YGTFEFFYLPFFRERTFPDDDARLRGPFIIDDNNEGYESDAKEFHPDFALRWIHTLGD
                     FDIGAAFFRGTSREPRLIPRANQNEKIILDPFYDIIDQQSLDIQWTRDAWLLKFEGIT
                     RGGHGNRFVATVAGFEYTLFQIFGSAADLGLLSEYLYDGRDSGAPPTVFDNDIFGGFR
                     LSLNDIQDTEFLAGIVTDTENAELFTFVETSRRFGQNWVAEIEARFFFNSDTNSIATG
                     ISNDGFFTFTISRFF"
BASE COUNT         1442 a          792 c          899 g         1325 t
ORIGIN      
        1 aaagggactc ataattatta gcgttatttg taaaacggag agtaaattaa atggcggata
       61 atagcagctt tgtactaaga tatacaaatt gggttattag atggcgatgg gctgtattga
      121 tattaagttt actgcttgcg attttgcttg gaactggggt aaagcgtcta ggcctggcta
      181 ccgattacag agtttttttc agtgatgata accccgacct tctggcattt gatgcactag
      241 agaatatata cactaaaaat gataacgtct taattctaat taaacccgaa aatggtgatg
      301 tatttaataa agatacgata aaagctatat atgaaattac tgaagaagcc tggcaaacgc
      361 cctactccac aagggtagat tcaattacca attttcaaca tacatgggct gatggcgata
      421 atttaatagt tgaagacctt atagaagaaa agaattttac tgaaaaagat attgccaggg
      481 caaaaaaagt agcattctct gaaccattta tatacaaaag attactttca gaagacggta
      541 aaaccgcggg cataaatgta cgcatacaac aacccgggaa atcccctgca gaagtgcccg
      601 agattgcggc ttttgcaagg gatctaaggg ataaatatag agctttatac cccaaccagg
      661 acattaggct agtgggcgta tctatgatga acaacgcctt tgccgaaact cctattaagg
      721 acttctcatt tgtaatgccc cttatgtttg gagtttttac ttttgcgctt ataatatttc
      781 taaggtcttt tctggcttca ggagctacac ttattctggt agtaatttct gctcttactg
      841 cccttggaat agcggggttt tttggcacat ttctagatcc cacaagttca tcaacaccta
      901 acatcatact aactcttgca gtagcagatt ctatccatat cctggtttcg ttctttgaag
      961 cacgaagaag cggtaaaaac agatatgacg ctattattga aagcatagat attaacgcaa
     1021 aaccagtatt tcttacaagc attacaactg caattggttt tttaagcctt aattttagcg
     1081 attctcctcc attccgcctt cttggaaacc tcacagcact tggagttatg attgcctggt
     1141 tttattcgat gacatttctt cctgcatttc tttcggttct tccgatcaaa gcccctaaac
     1201 gctcagagct tacatacagt tttattgata aacttagtga ttttgtaatc tcgagaccaa
     1261 aacaaatact gctattattg ggatcttttg taatagtctt aatattgcta atacctacaa
     1321 tgaatattaa tgacaggtac catgaatact tcagtaaaaa actcgatata agaaactcaa
     1381 tggagttttc agtaaaaaac ctaagtgggg tttatataac cagtttttct cttgaatcag
     1441 gtgatacaaa cggagtaagt gatcccgagt atctttcaaa agttagcgca tttacagatt
     1501 ggttaagggc caggcccgag attgcacacg taaattcatt tagtgatacg atgaaaaggc
     1561 taaacaagaa catgaatggc gatgatccgg ctttctacaa actaccggag aacagagagt
     1621 tgggcgctca gtacttgctg ctctatgaaa tgtcacttcc atacggcctg gatgttaacg
     1681 atcaaataaa tatcgataaa agttcgctca ggctggacgt aacctatgca gatgctaacc
     1741 ttaatactat cgtacaaaac agcaaagaag cgaataagtg gataaatgaa aatggaacag
     1801 aaagcatgaa ggaaaacagg ggtgcaggta ctacgcttat gtttgggaat attaccagaa
     1861 ggaacctcga tgcaatgctt ataggaacat ttgtgggatt tggtcttata gcaataatcc
     1921 ttatgattgc acttagaagc gtaaaagtgg gtcttataag ccttatacca aacatacttc
     1981 cttctgctat ggcttttggt gtatgggcgc ttcttatcgg cgaagtggga tttgctatat
     2041 cagtagttgc cagtatatca ataggtatta ttgtagacga tacagttcac ttccttacaa
     2101 agtatcacag ggcaagaaca gaacttggcc atgatactcc cgatgccata aggtatgctt
     2161 ttcatactgt aggaacagca atattaggga catcatttat tattgcggca gggtttgcca
     2221 tgcttggcat gagtacattc agggtcactt cttatatggg gctcttaact tctcttgcca
     2281 tagtatcagc attaattaca gacttcttac ttttaccagc actattactg atattagata
     2341 aagacaagaa aaaagataaa ggcactccat ttgcaaccaa ttaattaaat tatagttatt
     2401 ttataaaagg agaatcaaat gaaatttaaa atattaatgc taattttatc aatcttaatc
     2461 cccctttctt cggtttactc tcaaacatca gaagaaaagg gaaagcagat tgcaatagat
     2521 gctgataaag ctgatcaggg ctggggtgat aataaatcag tatcaaagat ggtgcttaga
     2581 aataaacaag gacaggagag tgtaagaaag cttaggagac tagcgcttga aattaacgag
     2641 cccggccttg gtgataagag cattacagta tttgatgaac caagagatat tgagggcaca
     2701 gcccttctgt ctcatacaaa aattttaaaa ccagatgatc agtggcttta tcttcctgct
     2761 gtaaaaaggg ttaagagaat ttcttcaaag aataaatccg gtccttttgt aggaagtgaa
     2821 tttgcttatg aagacctcct ctcacaagag gtagataaat acaattacaa gtggattaaa
     2881 gatgaaaaat gcggtgagct cgaatgtttt gtagtagaaa ggtatccact ctatgaaaat
     2941 tcaggatata ccaaacaggt tgtatggtgg gacaagcagg aatacagacc acagaagatt
     3001 gatttttatg acaggaagga ttcacttcta aagacactta catatactaa atataatcaa
     3061 tatttagata aatattggcg tgcagacttg tttgtcatga aaaaccatca aaatggtaaa
     3121 agcacagata tagtatttga aagctgggaa tttagacttg gacaagatca tagccagttt
     3181 accgcatcaa gactaaaacg tgccaaataa tttttcgtac cactttctaa cattattttt
     3241 attagtttcg tatttcctgt ctggcaaaca aatttatgcc gggcagtggg acattagggg
     3301 tgaaacaggc atagaactca gaatatttcc ggatgatcct gagtttaaca gccaggatga
     3361 tacatacttt tctccctcat ttatgatcga gcctgagata ttgtatgaaa ccgaaaaagg
     3421 aaatgacctt tttgaatttg tacccttttt cagatttgat ctggatgatg acaacaggac
     3481 tcactttgat ataagagagc taaactggtt ccatcaggga agcaattggt cgttactagt
     3541 cggtgcagat attgtttact ggggtgttac tgaatcaaga catcttgtta atataataaa
     3601 tcaaaacgat caggtcgaag acatagacga agaagacaaa cttgggcagc ctatgattaa
     3661 cttcacttat gacagtgatt acggaacatt tgaatttttc tatcttccgt tttttagaga
     3721 gcgcactttc cccgatgatg acgcaagact tagaggcccg tttataattg atgacaataa
     3781 cgagggatat gaatcggacg ccaaggagtt ccatcccgac tttgcactca gatggataca
     3841 taccctgggt gactttgata tcggagctgc attttttaga ggtacaagca gagagccaag
     3901 actaatccca agagccaatc aaaatgaaaa gataatacta gacccgtttt acgacattat
     3961 agatcagcag agccttgata ttcagtggac aagggatgcg tggctgctta aatttgaagg
     4021 aataacaaga ggcggtcatg gcaacagatt cgtagctacc gtagcaggat tcgaatatac
     4081 tctttttcaa atatttggaa gtgctgctga ccttggatta ctttcagaat atctatatga
     4141 cggcagagat tccggcgcac ctcctaccgt ttttgataac gacatattcg gtggtttcag
     4201 gctttcacta aacgatattc aggatacaga gtttttagca ggtatcgtca cagacacaga
     4261 aaatgctgaa ctatttactt ttgttgaaac atccagaagg tttggtcaga attgggttgc
     4321 agagattgaa gcgcgttttt tctttaactc tgatacaaac agcatagcaa ccggtattag
     4381 caatgacggt ttttttactt ttacaataag caggttcttc tagtttattt aacaattatt
     4441 ttgcgctaat aatattat
//
LOCUS       DBNGSV010000388        13510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate MAG05330
            SRR25158437_k127_415449, whole genome shotgun sequence.
ACCESSION   DBNGSV010000388 DBNGSV010000000
VERSION     DBNGSV010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414738
            Sequence Read Archive: SRR25158437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Pseudomonadati; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:23:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,724
            Genes (coding)                    :: 2,707
            CDSs (with protein)               :: 2,707
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 12 of 17
            Pseudo Genes (internal stop)      :: 2 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158437_k127_415449"
                     /isolate="MAG05330"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.23 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3388
                     /locus_tag="AC159U_13800"
     CDS             <1..3388
                     /locus_tag="AC159U_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3338169.1"
                     /translation="YSKGGFEQDLGTVVKKCESACSDYLDVLDTMLKEELLDPVAESL
                     KTLKNKYEQFLELQINKLPDTNFGIAHLKIGQIFMQLSNMESEDDDLQACIKSFEKAI
                     SILDSKTNKDQYSLTLKNLSDLYLRISEESRNEKDINKCINYSKEYQSLFPGSYAETN
                     YNEVSQNLNTATKLLDDVNKGYEALEEKLSIKDTKEEKQDKPKEQADSKSEEAEKVEE
                     PIQEDQGEEEKQAEPQSITEDSVETDEEIEELDEIEILDVEHIIDEDEQIVDEEDIQQ
                     PIQFSESAKDEPKIENVEDEIKNYENKLRTTSKKDNPEDYADSLYILGNAYLQLAKND
                     NDLDAYSKAAEAFRESLSYYTKTVDKQRYGLIKKSLGDIFTKYAEVEDKVKNLREGIK
                     LLEESYEILKESGTKDSVKDNLLILARAHFSISEQEGQNDDYKTSLGYSTKALTLMDD
                     SEKLSSAQTSRLIADTYRAINEQSSLTSNFKNSVEHYKKTLIYYTKTDFPDDYANIYD
                     SMGSCYKVLAELEDSSKNLAESINCYNEILGVYDLEKSPQQYAATENNLGIIYRALSE
                     VENKAENCKKAIDSYEKTLKVYNIGDYPQQYGAIQNNLGAAYRSLSEVENKAPNCKKA
                     INSFREALKVRTIDEYPAYHASTQNNLAATYLTLAEAEDSKANNKNAINAYEDSLKVF
                     TFKEFPLEHGIIQKNIGDIYFDNSAGDDKKDIKKALSCYESAIISYDKDAYAEEYSEL
                     SNKVGEIYLTFTEKDERIKNLNEAIKYFEGAHSCLSKDDHKYEYIVTCKNLSVGYLEL
                     GEEQDDVEKYKKAREFGEEALSHINDKDSKEFGETTKCLGSVYYDIGAETGDLESYHK
                     CLESFNSSLKYFTKEDHEEDYATIKDNLATCYKTLSEYENEAANCNKAIECYMDVLKV
                     YSSEESPIQYAATQNNLGVTYRTLAEYENQAENCKKAIDSYEQTLKVYDFDNFPLQYG
                     AIKNNLGAAYKTLADVENKVGNSNKAIDCYNEALKVRTFDAFPMHFAATQNNLGVAYR
                     TLAEEEGRAENSKNAIRAYESALEVYTYRNFPVQFASVQNNIGMTYRTLAEIEDKLGS
                     CQKGIKAFEEALSVFTADEHPDTYELVKANLDDITKFEMS"
     gene            complement(3413..4669)
                     /gene="hisS"
                     /locus_tag="AC159U_13805"
     CDS             complement(3413..4669)
                     /gene="hisS"
                     /locus_tag="AC159U_13805"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3338170.1"
                     /translation="MKTQTIRGFKDTLPENIKRWHFIEDTAKQILELYGFSEIRPPLL
                     EFTEVFTRGIGNTTDIVEKEMYTFEDRDGSSITLRPEGTASVVRSYIENSMHLKSPIN
                     KLYYLGPMFRHERPQKGRYRSFSQIGAELFGSYDPQYDAEAIIMLWEIFEKSSIGSAL
                     QIELSSLGDDKCRPHYKEEITKVLKAKENELCDDCKRRLSTNPLRILDCKKESCSKAT
                     ADLPSILDFLCEECDIHFKRVRELLDRMEVEYVINPKIVRGLDYYNRTVFEVTTDALG
                     SQNAVAAGGRYDGLVKQLGGQDTPAIGFATGIERMILLHEKLHPAGFNKRTDIYIAYL
                     DNKYEDFAMKTAFDLRQKGLTVEVDYNDRSLKSKLKRANKLEAKYTVIVGEEEFERGK
                     VKIRDMQSSSEEEVDLTAIQKFEFNQ"
     gene            4790..5914
                     /locus_tag="AC159U_13810"
     CDS             4790..5914
                     /locus_tag="AC159U_13810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3338171.1"
                     /translation="MKIIYVGSPPLLTRGASAIHMFKMCQAFGQLGHDITLFLPPYDN
                     KVNIYSYYGVEKNFDIKTILSTKNFLCQIIHGLSCIIHFAVNKPEYDIVITRNIIFTF
                     FSTVFLRIPTIYDAHHPLVNRAAEFMFNTFKDSRYLVRFMTNSGGLADYYIDKGLPTA
                     KLKVLHNGVELDKYKRGKTIKQARKLTKLPEDKQVVLYSGNIYKGRGIEQLIEASESF
                     KQALFYVLGGEQQDIDRYEKLLTDKKVNNFKFLGFVDQDKVKDYLYSADVLVMPYGKS
                     MTIGPGTQAAEFTSPIKLFEYMASKRVIVATMINSVMEILEDGKNAILVEPDDTKSLA
                     KGIRKALGNTENSAKLSGQAFSDVQNYSWQQRAQRMIENV"
     gene            5993..7498
                     /locus_tag="AC159U_13815"
     CDS             5993..7498
                     /locus_tag="AC159U_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HIR3338172.1"
                     /translation="MGRKWFLILLAAIMALGSFSSPNLALHAEELSEQDSHIESDHES
                     QTDTDNQTEYDDKYPSEPSNKDFLKDTAYMYGGLWAGRFFYVRNKNSRIFDTSFSKWI
                     DHITEWPETDDGDEFFTNFVTHPYIGSMYYLYYRERGHSRLKSAFGSVVQSTLFEYTI
                     EGLVETPSLPDLIFTPLVGTPLGIIFEETSKWLSGRDNAAVRSLAYVVNPMKIIINDR
                     KFGFVNPLSGTFGFHGTFEPKENKEHALRLAYSTFLESPIPVGRVMTATEVVNVRQNF
                     GGEFIMYYIRADLPNDSNTAAVYFRLSQAGINSININGNDPVSDGFELANLLLGAKQL
                     IYNSSNKALSMGFEFIVPTAYKDNVDRLQTLINFQRDLPYYLESAFTTSPYVSGAVWN
                     EHLALQANVGTDIIINSDKFEDDNVEWRIKYAASLAAELPIEYDPTFHIDFLGIYIPT
                     ADSIKNNDLFFAPGFRIGKKYDAGFSMQFPLTGPTDDVAKFSFVFDLQARF"
     gene            complement(7511..8089)
                     /locus_tag="AC159U_13820"
     CDS             complement(7511..8089)
                     /locus_tag="AC159U_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR3338173.1"
                     /translation="MMKNTAIGLSIIIVAVTVMACARAQGHSPAQKRQYTQDMKSDTL
                     QDLYRRKPETRSLVQNAAGYGVFSNINTQLLWFGAGNGYGVVVDNRTGKKTYMSMGEA
                     GVGIGVGLKDFREVIIFNNRAKLNNFVTSGWNVGTAQASAEAKYESDGASAAGEANLD
                     ADVVVYQMTEKGISLRVNFGASKYWVNDELNY"
     gene            complement(8154..9092)
                     /locus_tag="AC159U_13825"
     CDS             complement(8154..9092)
                     /locus_tag="AC159U_13825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3338174.1"
                     /translation="MSRLATISPVWGCSVDDLRALAKIAEDGGFEGIFSPEVPPWSAI
                     SNAQVFAEATSKIKVGTWIANIYMRQPVMAAATALTIQEISGGRMILGLGVSHKPVND
                     RYEIDMGDPMESMRDFVTAVKSFADGSNPALTLKRELPKLPVYTAGLTKGAATVAGEV
                     ADGLMPYMATPEYISTLKGYVADGAKSAGRDPSEIDITNGIPSFVSDDLDAAIKSAKR
                     GLSGYARFPFYQRLIRNIGFGDVVDQIQGGANPADVFSDELVEAFALVGPADKCKARL
                     QDFYNAGVDLAIIVPGPVGKQSNVEVMENTVKAYAG"
     gene            complement(9120..10016)
                     /locus_tag="AC159U_13830"
     CDS             complement(9120..10016)
                     /locus_tag="AC159U_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3338175.1"
                     /translation="MIEYIQPIFRPPSEGKSFLLQATIGCSHNNCTYCAMYRKDEQKF
                     RIRPMYEIIAIIDEASSSGLIEDRVFIADGNALVLAQKKLVEIMQYLGEKCPQLTRIR
                     MYANVGDILRKGVDNLKELKELGLDMVYIGFESGDDVVLERIKKGASHVQTVQASKIL
                     KEAGIENSAMVLLGMGGKERSEIHARETGRLLTGCDPEYVGALSLQLRKGAPLYKEWD
                     EGNFELPDKFQMIRELKIIVENTELTGGYFFANHISNYLPIKAQFPRDKEKVLEEINK
                     VLKTEDESLLRPDFYRDVINQY"
     gene            10157..10657
                     /locus_tag="AC159U_13835"
     CDS             10157..10657
                     /locus_tag="AC159U_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338176.1"
                     /translation="MSAVFVLFGEKKQVEQEISNLSDKVNDLKEINDNENENEKENYI
                     QSTSRELPSKPDNLTRPKPPDYQRTVNKDRKLVARGYIMLDENNLCDITNKEPEVQII
                     PYHDSSGTLNNNSEYVDCVLNIYDLENGYHTLAITVDHLSDLTTKKQLYIRQDLISYI
                     DIQLKY"
     gene            11424..12710
                     /gene="hflX"
                     /locus_tag="AC159U_13840"
     CDS             11424..12710
                     /gene="hflX"
                     /locus_tag="AC159U_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3338177.1"
                     /translation="MPGRFCIAHVLPPNPQKIQEQMWNRESYNDLGRVDTDFEQFVLD
                     LETELERKYVDLGGKQNRHGIVLVGVSTTGRVDAETSLSELELLAQSANKIILDKFIQ
                     TSKKIDPRYLVGKGKLKEITLRARHIGADTLVFNKELTPAQVRAINKETDLEIVDRTQ
                     IILEIFAARATTSEGKLQVQLAQAQYELPRLVGKGVEFSQLGGGIGTRGPGEKKLEEQ
                     RRTLRKQIDILEKKIDELSLRRSHTRKQRMSHGISTVTFIGYTNAGKSTLFNSLTKGH
                     VLAENKLFSTLNPTTRKLMLPSGRQILITDTVGFISELPAELVNAFRATLEELGESSV
                     LIHVADISDPMLDEKIESVEKILQDTGYNTIPRINILNKSDKVTKEIAENISKRYGAI
                     AVSAKQKDTAGFYELLETKLSELDNGQIVVSKEIAL"
     gene            complement(12957..>13510)
                     /locus_tag="AC159U_13845"
     CDS             complement(12957..>13510)
                     /locus_tag="AC159U_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3338178.1"
                     /translation="VEFLDNKPMMATLRPNVFKAEESGGAGTVVNATVGLSADDIKTK
                     IISVDRVESTRPELTEADRIVSGGRGMGNGDNFSIIYDLADSMGAAAGASRAAVDSGF
                     VPYEMQIGQTGKVVSPTLYVAVGISGAIQHFAGMGSSKVIVAVNKDPDAPIFQKCDYG
                     ICGDLFEVTPALTEEFKKLLSQQ"
BASE COUNT         4335 a         2613 c         2626 g         3936 t
ORIGIN      
        1 ctattcaaaa ggcggattcg aacaagacct gggcacagta gtgaaaaagt gtgagagtgc
       61 ttgcagtgat tatttagatg ttttagacac aatgttgaaa gaagaattac ttgatccagt
      121 tgctgaaagt ctaaagacac taaaaaacaa atatgagcaa tttttagaat tgcaaattaa
      181 caaactgcct gatacgaatt ttggaattgc acatttaaaa attgggcaga tattcatgca
      241 gctttccaat atggaaagtg aggatgatga tttgcaagcc tgcataaaat cttttgaaaa
      301 agcaataagt attctggatt ccaagacaaa taaggatcag tattcattaa ctcttaaaaa
      361 tctaagtgat ctatatctga ggatttccga agagtccaga aatgaaaaag atataaataa
      421 atgtataaat tattcaaagg aataccagag cctttttccg ggaagttatg cagagaccaa
      481 ttacaatgaa gtatctcaga atttaaatac agctactaaa cttctggatg atgttaacaa
      541 gggttatgag gcgctcgaag aaaaacttag catcaaagac actaaagaag aaaaacaaga
      601 taaacctaaa gaacaagcgg atagtaaaag cgaagaggca gaaaaggttg aagaacctat
      661 acaagaggat caaggagagg aagaaaagca ggctgaacca caaagtatta ccgaagacag
      721 tgttgagaca gatgaagaaa ttgaagagct agatgaaata gaaatactcg atgttgaaca
      781 tataattgat gaagatgaac agattgttga tgaagaggat attcaacaac ccatacaatt
      841 tagcgagtct gccaaggatg agccgaaaat agaaaatgta gaagatgaga ttaaaaatta
      901 tgaaaacaag ctaagaacca ctagtaaaaa agacaacccc gaagattatg cagacagcct
      961 ttatatactc ggaaatgctt atctgcagtt agctaaaaat gataatgacc tggatgcata
     1021 ttccaaggca gcagaggcgt ttagagaatc attatcgtat tatacaaaga cagttgataa
     1081 gcagcgttac gggcttataa aaaaatctct gggcgacata tttacaaagt atgccgaggt
     1141 agaagataaa gtaaaaaacc ttagagaagg catcaagctt ctcgaagaat cgtatgaaat
     1201 actaaaagag tcgggtacaa aagattcagt aaaagataat ctattaattc ttgcaagggc
     1261 acatttttca atttcggaac aagaagggca gaacgatgat tataagacct cgcttggcta
     1321 ttctactaaa gcgcttactc ttatggatga tagcgaaaaa ctgtccagtg cgcagacaag
     1381 cagacttata gctgatactt atagagcaat taatgaacaa tcttctctca cttccaattt
     1441 taaaaattcg gttgagcact acaaaaaaac attaatatac tatactaaaa cagattttcc
     1501 tgatgattat gcaaacatat acgacagcat gggttcttgt tataaagtac tggcggaatt
     1561 agaggacagc agtaaaaacc ttgccgaatc aattaactgc tataatgaaa tcctgggcgt
     1621 ttatgatctg gagaaatctc cacagcagta tgcggctaca gagaataacc ttggcataat
     1681 ttaccgggcg ctttccgaag ttgaaaataa agctgaaaac tgcaaaaaag caatcgactc
     1741 ttatgaaaag acgctaaaag tttataatat aggtgactac ccacagcagt atggtgcaat
     1801 acaaaacaat ctgggtgctg catacagaag cctttctgaa gtagagaaca aggctccaaa
     1861 ttgtaaaaaa gccattaatt cttttagaga ggctcttaag gtaagaacaa ttgacgaata
     1921 tcctgcctac catgcatcta ctcaaaacaa tctcgccgct acttacctaa ctcttgcaga
     1981 ggccgaagat tccaaggcaa ataacaagaa tgctattaat gcatatgagg actcccttaa
     2041 ggtttttaca tttaaagaat ttccactcga gcacggtatt atccaaaaga acatcggaga
     2101 tatttatttt gataactctg ccggtgatga taaaaaggat atcaaaaagg cgcttagctg
     2161 ttatgagagt gcgattatta gctatgataa ggatgcatat gccgaggaat atagcgagct
     2221 tagcaacaag gtaggagaaa tttatcttac ctttaccgaa aaagatgaaa ggataaaaaa
     2281 tctaaacgaa gccataaaat attttgaagg agctcatagc tgtctaagta aggatgatca
     2341 taaatatgag tatatagtta catgtaaaaa cctgagtgta ggttatcttg agcttggtga
     2401 agagcaagat gatgtagaaa aatataagaa ggcaagggag tttggagaag aggcgctttc
     2461 gcatataaat gataaagatt ctaaagagtt tggtgagacc actaaatgtc tgggtagtgt
     2521 ttactatgat ataggggcag aaacgggtga ccttgagagc tatcataaat gcctggaatc
     2581 atttaactca agccttaaat attttacaaa agaagatcat gaagaggact atgcaactat
     2641 aaaagacaat ctagctacat gctataagac tctttccgaa tatgaaaatg aagcagctaa
     2701 ctgcaacaag gcaatagaat gttatatgga tgtactaaaa gtttacagct cggaagaaag
     2761 cccaatacaa tatgcggcta cacaaaataa tctcggggta acatacagaa cgcttgcaga
     2821 atacgaaaat caggctgaga attgcaaaaa ggccatcgat tcatatgaac aaactctaaa
     2881 agtgtatgac tttgataact tcccgcttca gtatggagca ataaaaaata acctgggggc
     2941 agcttacaaa acactagctg atgttgaaaa taaagtcgga aacagcaata aagccatcga
     3001 ctgttataat gaggctttga aagttagaac ctttgatgca ttccctatgc attttgccgc
     3061 tactcaaaac aatcttgggg ttgcctacag gactcttgca gaggaagagg gcagggcgga
     3121 aaactcaaag aacgctatta gggcctatga gagcgcgctt gaggtctata cctacagaaa
     3181 cttcccggta cagtttgcat ctgtacagaa caacatcggt atgacataca ggactcttgc
     3241 cgagattgaa gataagcttg gcagctgcca aaaaggaatc aaggcatttg aagaagcgct
     3301 tagcgtattc actgcggacg aacaccccga tacctatgag cttgtaaaag ctaatttgga
     3361 tgatatcaca aagtttgaga tgagttaagc tttatatgat ttgtaaatca atctattgat
     3421 tgaattcaaa tttctgaatc gcagttaaat ccacctcttc ttcactgctc gactgcatat
     3481 ctcttatttt tactttgcct cgctcaaact cctcttcccc aacaatgaca gtatattttg
     3541 cctcaagctt gttcgctctt ttaagcttgc tcttaaggct cctgtcattg tagtccactt
     3601 ctactgttaa acctttttgc ctgagatcaa aagcagtctt catggcaaag tcttcgtact
     3661 tattatcaag ataggcaata tatatatctg tccttttatt aaaacctgcc ggatgcagtt
     3721 tttcatgcag cagtatcatc ctttctatac cggtagcaaa accaatggca ggggtgtcct
     3781 gtcctccaag ctgttttaca agcccgtcgt atctgccgcc ggcagcgacg gcgttttgtg
     3841 atccaagtgc atcggttgtc acttcaaaca cggttctgtt atagtaatca agccctctca
     3901 ctattttagg attaatcaca tattcgactt ccatcctatc aaggagctct cttactcttt
     3961 taaaatggat gtcacactcc tcacacagaa aatcaagaat cgaaggtaga tcggctgtag
     4021 ctttactgca agactctttc ttgcagtcaa gaattctaag cgggttagtg cttagtctcc
     4081 tcttgcagtc atcacagagc tcattttctt ttgccttaag tacttttgta atttcctctt
     4141 tataatgcgg tctgcacttg tcatccccaa gactgctgag ctcaatttgc aatgcagagc
     4201 ctatgctgct tttttcaaaa atttcccata gcattattat tgcctcagca tcatactgag
     4261 ggtcataaga gccaaataac tcagcaccaa tctggctgaa gcttctataa cggccttttt
     4321 gcggcctttc atgcctgaac ataggcccaa gataatatag tttattgata ggagacttta
     4381 gatgcatcga gttttctata taagaacgca ctacgcttgc cgtgccttct ggccgcagcg
     4441 taatggaaga gccgtcgcgg tcttcaaatg tgtacatctc tttttcgact atgtctgttg
     4501 tattccctat tcctcttgta aatacctcgg taaattccaa taaaggaggc ctgatttctg
     4561 aaaagccata tagctcgaga atttgctttg ccgtgtcttc gataaaatgc catcttttaa
     4621 tgttctccgg cagagtatct ttaaaacctc ttatcgtttg tgttttcata ttttatataa
     4681 tccctgctgt cttgagtatg aataaatcag ctataaacta attctatttt gtaataaata
     4741 caatataaag gttaaattat atcttttaaa ttaaacaggg actataaaat tgaaaataat
     4801 ttatgtagga agtcctccac ttctcaccag aggtgcaagc gcaattcata tgtttaagat
     4861 gtgccaggct tttggccaat tgggacatga tattactctg ttccttccac cttatgacaa
     4921 caaggtcaat atttattcct attatggagt agagaaaaac tttgatatta aaaccatttt
     4981 atctacaaaa aattttttat gtcagataat ccatggtctc tcatgtatta tccattttgc
     5041 tgtaaacaag ccagaatacg atattgtgat taccagaaac ataatattta catttttttc
     5101 tacagtgttc ttaagaatcc cgacaatcta tgatgctcac catccccttg taaacagggc
     5161 tgccgaattt atgtttaata cattcaaaga ttctcggtac cttgtgcggt ttatgactaa
     5221 ttcaggcggc ctggcagatt actatattga taaggggctt ccaactgcaa aactgaaggt
     5281 tcttcacaat ggcgtagagc ttgataagta taaaaggggt aaaactatta aacaggcaag
     5341 gaagttaact aaattgccgg aagataaaca agtagttctt tactcaggca atatttataa
     5401 gggcagagga attgagcaat taatcgaggc ttctgagagt tttaaacaag cgctatttta
     5461 tgttttaggc ggtgagcagc aggatattga tcgctatgag aaacttttaa cagacaaaaa
     5521 ggttaataac tttaagtttc ttgggtttgt ggatcaggac aaagtgaaag attatctata
     5581 ctcggcagat gtattggtaa tgccatatgg aaaaagtatg acaataggac ctggtacaca
     5641 agcagcggaa tttacttcgc caataaagct ttttgaatat atggcatcaa agagagttat
     5701 tgtagcaacc atgataaaca gtgtaatgga aatattggag gatggaaaaa atgcaatatt
     5761 ggttgaaccg gatgatacaa aatctcttgc aaaggggatt agaaaagctc tgggtaacac
     5821 tgaaaattct gctaaacttt cgggacaggc tttttcagat gttcaaaatt attcatggca
     5881 gcaaagggca cagaggatga tagaaaatgt ataatttcca tttttgtaaa cttctaggta
     5941 tcttttctgg aatttttaat atcttataca tattgtaata atatatttgg ctatgggaag
     6001 aaagtggttt ttaatattac tggcagcaat tatggcgctt ggcagcttta gctcaccaaa
     6061 tttagcgcta catgcagaag aactttctga gcaggattca cacatagaat cagaccatga
     6121 gtctcaaact gacactgata atcagactga gtatgacgat aaatatccaa gtgagccttc
     6181 aaacaaggat ttcttaaaag acactgcata tatgtatgga ggtctatggg ccggaaggtt
     6241 cttctatgta agaaacaaaa atagcaggat ttttgataca tcattttcaa agtggataga
     6301 tcatatcact gaatggccgg aaactgatga tggcgatgag ttctttacaa attttgttac
     6361 tcacccttat atcggttcta tgtattatct atactacaga gaacgcgggc actcaagact
     6421 aaaatctgcg tttggctctg tagtacaaag cacgcttttc gaatatacta ttgagggtct
     6481 tgtagagact ccatcgctcc ctgatcttat ttttacccct cttgttggaa cacccttggg
     6541 aataatcttc gaagaaacct ctaagtggtt gtctggcagg gataatgcag cagtaagaag
     6601 ccttgcttat gtagtaaacc caatgaaaat tataataaat gacagaaagt tcggatttgt
     6661 aaatccgctt tcgggaacat ttggctttca tggtaccttt gagccaaagg aaaacaaaga
     6721 acatgctcta agacttgcgt actccacatt tcttgaatca ccaattccgg ttggaagagt
     6781 aatgaccgca actgaggtag taaacgtaag acagaacttt ggcggcgagt ttataatgta
     6841 ttacattagg gcagatcttc caaatgattc aaacactgct gcagtatatt ttcgtctttc
     6901 ccaggcaggg attaatagta ttaatattaa tggaaacgac ccggtcagtg atgggtttga
     6961 gcttgcaaac ctgcttttgg gagctaagca attaatttat aatagtagca acaaagccct
     7021 ttccatgggg tttgagttta tagttcctac agcttataag gacaatgtag acaggctcca
     7081 aacacttata aattttcagc gtgatctgcc atattatctc gaaagcgcat ttactacaag
     7141 cccatatgtt tcaggtgctg tttggaatga gcatttagct ttacaggcaa atgtagggac
     7201 tgatatcatt ataaattccg ataagtttga agatgataac gtagaatgga gaattaaata
     7261 tgcagcctcc ctagctgctg agcttccaat tgagtacgac cccactttcc atatagactt
     7321 cctgggtatt tatatcccta ctgctgattc tataaagaac aatgatctat tctttgcccc
     7381 cggatttaga ataggtaaga agtatgatgc cggatttagt atgcagtttc cgcttacagg
     7441 gcctacagat gatgttgcca aatttagctt tgtctttgat ctgcaggcaa gattctaaat
     7501 actttctata tcagtaattt agctcgtcat ttacccagta ctttgatgca ccaaagttta
     7561 ctctaagcga tatgcctttt tctgtcatct ggtatacaac cacatctgcg tccagattgg
     7621 cctctcctgc agcggaagct ccgtcacttt catacttggc ctcagcactt gcctgagccg
     7681 ttccaacatt ccagcctgag gtaacaaagt tattaagctt tgctctgtta ttaaaaatta
     7741 tgacttccct aaaatcctta agacctacgc caatgccgac ccctgcctca cccatactca
     7801 tatatgtttt ctttcctgtt ctgttatcaa ctaccacacc atagccgttt cctgcaccaa
     7861 accataatag ctgagtatta atattactga acactccgta gccagctgcg ttttgaacca
     7921 ggcttcttgt ctcgggtttt ctcctgtaaa gatcctggag agtatcgctt ttcatatcct
     7981 gtgtatattg tcttttctga gcaggactat gaccctgagc cctggcacat gccataacag
     8041 ttacagctac aataataatt gataatccaa ttgcagtatt tttcatcatt attccccctg
     8101 ttaattaata gacgggctta aaaagcccgt ctaaaattat atgctttatt tttttaacct
     8161 gcgtatgctt taaccgtgtt ttccataacc tctacattgc tctgttttcc aacagggcct
     8221 ggaacaataa tagcaagatc aacccctgca ttatagaagt cctgtagtct ggctttgcat
     8281 ttatcggccg ggccaacaag tgcaaatgct tcaaccagtt cgtcagaaaa tacatcggca
     8341 gggtttgctc caccctgaat ttgatcaacc acatcgccaa acccaatgtt tctgattagc
     8401 ctctgataga atggaaatct tgcatagcca gaaagccctc tcttcgcaga ttttatagca
     8461 gcatcgaggt catctgaaac aaaagacgga ataccatttg ttatatcaat ttcagatgga
     8521 tctctacctg cactttttgc accatctgcg acatagcctt ttaaggtgga aatatactcc
     8581 ggggtcgcca tatatggcat aagcccgtcg gcaacttcac ccgctactgt ggctgctcct
     8641 tttgtaagac ctgcagtata taccggaagc tttggcagct ctctttttag agtaagcgcg
     8701 gggttacttc catcagcaaa agacttaaca gctgttacaa aatctctcat cgattccatg
     8761 ggatcgccca tatcgatttc gtagcggtcg tttacgggtt tgtggcttac tccaagccca
     8821 agtatcatcc ttccgccaga gatttcctga atagtgagtg ctgttgcagc agccataaca
     8881 ggctgacgca tatatatatt ggcaatccac gtgccgactt ttattttaga tgtagcctct
     8941 gcaaaaacct gcgcgtttga tattgcactc catggcggaa cctcgggtga gaatattcct
     9001 tcaaaacccc cgtcttcagc tatttttgct agcgccctaa ggtcatctac gctgcatccc
     9061 caaactggtg aaattgttgc taatctagac atatttgtgc tccttttaaa taattgattt
     9121 taatattgat ttattacatc ccggtaaaaa tcaggacgca gtaatgattc gtcttcggtt
     9181 ttaagtactt tatttatctc ttctaaaact ttttctttat cccttggaaa ctgtgccttg
     9241 atcggtaggt aattggaaat atggtttgca aaaaaatagc caccggtaag ttctgtattt
     9301 tccacaataa tcttaagctc acgtatcatc tgaaatttat caggtagctc aaaattgccc
     9361 tcatcccact ctttgtataa gggtgctcct tttctaagct gcaaagaaag tgcacctaca
     9421 tattccggat cacaaccggt aagcagcctg ccggtttctc tggcatgtat ttcactcctt
     9481 tccttgccgc ccattccaag aagtaccata gccgagtttt ctatacccgc ttcttttaga
     9541 atcttagatg cctgtacggt ttgcacatga ctagcaccct tttttattct ctcaagcacc
     9601 acatcgtcgc cgctttcaaa gcctatatac accatatcta gcccaagctc cttaagctct
     9661 ttaagattat ctaccccctt cctgagtata tctcctacat tggcgtacat tctaattctg
     9721 gtaagctgtg gacatttttc tccaagatac tgcattattt ctacaagctt tttttgagca
     9781 agaacaagag cattaccatc tgctataaaa accctgtctt caattaggcc ggacgagctg
     9841 gcttcgtcta taatggctat aatctcatac attggacgga tacgaaattt ctgctcgtcc
     9901 tttctgtaca ttgcacagta agtacagttg ttatgggaac atccaatggt tgcctgaagc
     9961 agaaaactct taccctcgct gggtggtctg aatattggct ggatatattc tatcacccct
    10021 acaaaataac aaaattaggg ggttcttgca agaagaatta atctattaaa tctgctttgt
    10081 ttatagcaaa aatattacta taataaatta gatgtctcag gacctaaaaa taataacagt
    10141 tctattcatt ttaataatgt ctgcagtctt tgttcttttc ggtgaaaaga agcaggtgga
    10201 acaagaaatc tccaatttgt cagacaaagt aaatgatcta aaagagatta atgataacga
    10261 aaatgagaat gagaaggaga attatattca gagtacttcc agagaattac cttctaaacc
    10321 tgataactta accagaccta aaccacccga ttatcaaaga actgtgaata aggacaggaa
    10381 acttgttgca aggggttata taatgttaga tgaaaataac ctgtgtgata ttacaaacaa
    10441 agagcctgaa gtgcaaataa tcccttacca tgacagtagc ggaacattaa ataacaattc
    10501 tgaatatgta gactgtgtat taaatatata tgatcttgaa aatggttatc atactcttgc
    10561 cataactgtt gaccatttga gtgatttaac tacaaaaaag caattatata taaggcagga
    10621 cctaataagc tatatagata ttcagcttaa gtattaatta atcttataca gtcgcagcac
    10681 agtttagtaa tttttatata ggtagcgtgc aattctttac aaaacacaat tattatttat
    10741 aattcgtacc gttatggaag aacatgaatt tataggattt atatatatac ttggtataat
    10801 tggttttatt ggattaatcc tcgttttagt ttctctaacc cacgtagtcc tgtaggttat
    10861 ttaagctttg agtaatactt cattgttaat accttaatac cacagtaata gcacccggaa
    10921 ttttatctat ttcagcacag ttttattcaa ttcatactga caatcgcatt ccagcctgtt
    10981 tgaatttaat gttttatgac ttatatttaa ctatcatcta acatcagggg ttgtaagata
    11041 tagaaaaaat actaggcaat ttaaaaggac taaaaaagag tgaccttaag aatctctcaa
    11101 gacttgcaaa gagaaaagtc cctccaaaag aaattataac cctcgagctt ggaagaacgc
    11161 ttgctgcagt atctggggaa cttaaaagaa acatcaccct cataataagc agaagtggta
    11221 aaatcgagca tatatatctg gggcttccag aagatataga ttttacatac attctcggcc
    11281 ggtcaagaga atccaactac aggctaaggg gttacagagc catatattca agccttaagc
    11341 acgatgagct tactgcaaac gacctttcca ctttattaac tgagaggctt gatttaattg
    11401 gtcttctaag agtaaatgac ggcatgccgg gcagattttg tatagctcat gtccttcctc
    11461 caaatcccca aaaaatacag gagcagatgt ggaatagaga gagttataac gatctgggac
    11521 gggttgatac ggactttgag cagtttgtgc ttgatctgga aactgagctt gaaagaaaat
    11581 atgtcgatct tggaggaaaa cagaaccgtc acgggattgt gcttgtgggg gtttcaacta
    11641 caggcagggt ggatgccgaa acatcactta gtgagctgga gcttttggca cagtctgcaa
    11701 ataaaatcat cttagataaa tttatacaga cttcaaaaaa gatagacccc agatatctgg
    11761 tgggaaaggg aaagctaaaa gagattacac tgcgggcacg gcacattgga gccgacactc
    11821 tcgtatttaa taaagagctt accccggccc aggtgcgggc aataaataaa gagactgatc
    11881 tggaaattgt agaccgcact cagatcatac ttgaaatctt tgcagcaagg gctactacaa
    11941 gtgaaggaaa gctccaggta cagcttgccc aggcacagta tgagcttcca aggcttgtgg
    12001 gaaaaggagt agaattttct cagcttggag gtggtattgg tacaaggggg cctggagaaa
    12061 agaaactgga agagcagaga agaacgctta gaaaacagat tgatattctg gaaaaaaaga
    12121 ttgatgagct ttctttgcgc agaagtcata caagaaagca gagaatgtcg cacggcattt
    12181 ctactgtaac cttcatcgga tacaccaatg ccggcaagtc aacgcttttt aattcactta
    12241 caaaagggca tgtgcttgca gagaacaaac tgttttcaac gctaaatcct actacaagaa
    12301 aacttatgct tccatcggga aggcagatac tcataacaga caccgtagga tttataagtg
    12361 agcttcctgc tgaacttgta aatgcattca gggcaacgct tgaagagctt ggggagtcat
    12421 ctgtgcttat ccatgttgca gatataagcg atcccatgct ggatgaaaaa atagagtcgg
    12481 ttgagaaaat actgcaggat acaggatata acaccattcc caggataaat atcttaaaca
    12541 agtcagacaa agtaactaaa gaaatcgcag aaaacataag caaacgctac ggagcaatcg
    12601 cagtttcagc caagcaaaaa gatactgcgg ggttttatga gcttttggaa actaagttat
    12661 ctgaactaga taatgggcaa attgttgtta gtaaagagat tgcactctaa gaaattccat
    12721 catgtgatca tgactaatac atacttcgac tgtgctcagt gtgttgtgta ttcttttcaa
    12781 taattatttt atctcgaacg aatgtgagag atcttttttt acatcactta accaaagcag
    12841 agtaaaaaga atttctcata tgcattcgaa atgaattaac aaaagagtac aaaaaaaggc
    12901 gagctaccca aaaagataac ccgccttttt atccttaaag tttttttagt taataattat
    12961 tgttgtgaaa gaagtttttt gaactcttcg gtaagagcag gggtaacttc aaaaagatca
    13021 ccgcagatgc cgtagtcgca tttctggaaa attggagcat cggggtcttt gtttactgct
    13081 acaataacct ttgatgaacc catgccagca aaatgctgaa tagcacctga aattcctact
    13141 gctacataaa gagtaggtga tacaaccttt ccggtctgac ctatctgcat ctcataaggt
    13201 acaaaccctg agtctactgc tgcacgtgaa gcaccggctg cagcacccat ggagtctgca
    13261 agatcataga taatagaaaa gttatcaccg ttgcccatcc ctcttccgcc agaaacgatt
    13321 ctgtctgctt ctgtaagctc cggtcttgtg gattctactc tgtctactga gataatcttg
    13381 gttttaatat cgtctgcgct aagacccact gtagcgttta ctaccgtacc tgcgccacct
    13441 gattcctctg ccttaaacac gtttggtctt agagttgcca tcattggttt gttgtctaaa
    13501 aactctactg
//
